LOCUS       DAOPRQ010000001        69786 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_286_length_69786_cov_11.863432, whole genome shotgun sequence.
ACCESSION   DAOPRQ010000001 DAOPRQ010000000
VERSION     DAOPRQ010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 69786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 69786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69786
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_286_length_69786_cov_11.863432"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /locus_tag="PKJ61_00005"
     CDS             <1..622
                     /locus_tag="PKJ61_00005"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HOC12247.1"
                     /translation="ARVQSLARFRSDFEESLATGWAGFAGRLIERAAPRPAGVPTAYV
                     MQPFEVRLDYLGRPWCSVKFELGHDEIGDADEPEYQLASDLAALLTEVGLEPPKPVAV
                     MRADHQIAQKLHAASEAGSERARDLVDLQLLDRGEKLDLAQVASTCTRLFDYRQQQQW
                     PPIIVVGDQWDTLYVEAADGLDVLATVDEAVLWANEFIRRIAAART"
     gene            complement(723..798)
                     /locus_tag="PKJ61_00010"
     tRNA            complement(723..798)
                     /locus_tag="PKJ61_00010"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(763..765),aa:His,seq:gtg)
     gene            complement(1157..1786)
                     /gene="orn"
                     /locus_tag="PKJ61_00015"
     CDS             complement(1157..1786)
                     /gene="orn"
                     /locus_tag="PKJ61_00015"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017934743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-exoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HOC12248.1"
                     /translation="MNEHLVWIDCEMTGLDLKADALIEVAALVTDSELNVLGEGITVV
                     IKPSDTALDQMGDFVRTMHANSGLLPLLADGLEMAEAEERVLAYIREHVPESRRAPLA
                     GNTIGTDRAFLARDMPTLEGHVHYRNVDVSSLKELARRWYPRTYYNTPAKNGNHRALA
                     DICESIEELRYYRETLFVPTPGMPTDALREIAAKHQGSLTADLATATGS"
     gene            1864..3138
                     /locus_tag="PKJ61_00020"
     CDS             1864..3138
                     /locus_tag="PKJ61_00020"
                     /inference="COORDINATES: protein motif:HMM:NF012457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HOC12249.1"
                     /translation="MPGFSMAGCELGVASAATQIEGGQLDTNWHRWAASGGAVDGSSP
                     ARAADHWNRVSEDTSLLAEMGIGHYRLGIEWVRVEPSPGEFDPAAIGHYREELSGLRA
                     AGVRPLVTLHHFNLPGWLADAGGWLAPEATEAFLRFVRRMVTDLGDIVDEWIPINEPN
                     VYAVNSYLFGLWPPDEHSYRRTIRVLEILATAHIAAYELIHRLQPEAKVGTATHLRVF
                     APANPLNPWHQLAAHINDHLFQRAPLRAMSTGRFPAPLRRPSWVRPGRYYDFQGINYY
                     TRSTVSGLANGVASNVPVNDLGWEVFPSGLGEVAAWVHTAYPAPIYVTENGTADAADA
                     FRSRYLYDHLAVVAASGLPIRRYYHWCFTDNWEWAEGESARFGLVALDFESQQRMIRA
                     SGRFYADVIANRGVTTAAYQRWVAGQEYPTNG"
     gene            complement(3176..3718)
                     /locus_tag="PKJ61_00025"
     CDS             complement(3176..3718)
                     /locus_tag="PKJ61_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011758042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HOC12250.1"
                     /translation="MESISVVRDAFGRVHELVPAVLNGLSRDDLRWRPDPSANPIGWL
                     VWHLLRVEDDHLAPLAGFDQVWFQGWQERFDLPYAADAGGFGQSPEEVGQFDVPGADL
                     LLGYAKAVAVQTEQVLDALARDDLDRVVDTRWNPPVTLGVRLVSVVLDTAQHIGQAAY
                     VKGLRERLVGHDSGWQGYPE"
     gene            3778..3850
                     /locus_tag="PKJ61_00030"
     tRNA            3778..3850
                     /locus_tag="PKJ61_00030"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3811..3813,aa:Arg,seq:tct)
     gene            3892..5082
                     /locus_tag="PKJ61_00035"
     CDS             3892..5082
                     /locus_tag="PKJ61_00035"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOC12251.1"
                     /translation="MTASVDDRRTLAARNATMLVFAVNGFAFASWMSRVPDVREALAL
                     TPGELGLLLLSISAGALCGLPLAGRVTHRIGAQATVRVGMGLMVSGLVLAAIAAEAHA
                     GMYWVAPGLFLVGLGSGVWDVAQNVEGTIVERSLGRAIMPWFHAGFSGGTVLGALVGA
                     GMTALHVPVLVHLVTVAVLSALALWWGTLSFLPAFGESEDAPTSPARGKSAWLEPRTL
                     LIGLMVLAAAFAEGTANDWMAVAFVDGHNLDNAMGVVALAVFLTFMTAGRIFGTGLLD
                     RYGRVPVLRGLFAASIIACALVVFGDTWLAFIGVAIWGVGASLGFPVGMSAAADDPAR
                     AAVRISVVATIGYTAFLAGPPLLGFLGDHFGILRALLVVGAVSILAMLVVPAAKPLPP
                     NPES"
     gene            5135..6607
                     /locus_tag="PKJ61_00040"
     CDS             5135..6607
                     /locus_tag="PKJ61_00040"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012924074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HOC12252.1"
                     /translation="MIESREVELLAGVPEGLFIGGRWRSASDGGTFEVADPATGSPVK
                     RVASATVADGLAALDAACDAFPAWAKTPSRERAELLRRAFDLLQERKEEFALLMTIEM
                     GKPLAEARGEVAYGGEFLRWFSEEAARIQGRYGPNPEGTGRMIVSQHPVGPCYLITPW
                     NFPLAMATRKVAPALAAGCTVVVKPAALTPLTTLYFAKLLADAGLPDGVVNVVTTKAS
                     GPVSAAVLADPRLRKLSFTGSTPVGKELLKQAADGVLRTSMELGGNAPFLVFEDADLD
                     KAVDGAILAKFRNIGQACTAANRFIVHRSIAAEFAARVTARVREFRTGRGTEEGITIG
                     PLIDADAVAKVDGLVRDAVARGASVQTGGAPIDGPGTFYAATVLTEVPPDSSIVREEI
                     FGPVLAIVTFADEDEAVRLANDTGYGLISYVFTESNACGQRMIERLETGMMALNVGVV
                     SNAAAPFGGWKLSGIGREGGAEGIHEYLETKYTLTPNPFG"
     gene            6736..7209
                     /locus_tag="PKJ61_00045"
     CDS             6736..7209
                     /locus_tag="PKJ61_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HOC12253.1"
                     /translation="MSLQEFKAKTITGQDQALAAYLGQVILVVNTASKCGFTPQFAGL
                     EKLYADLRADGLVVLGFPCDQFANQELGSDAETAEFCQLNYGVTFPMFAKVEVNGRNA
                     HPLFAWLRSEQGGVLGDAIKWNFTKFLIDREGRVVRRYPPTVDPAAIEADVRALL"
     gene            7535..8386
                     /gene="ppk2"
                     /locus_tag="PKJ61_00050"
     CDS             7535..8386
                     /gene="ppk2"
                     /locus_tag="PKJ61_00050"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HOC12254.1"
                     /translation="MESEKPKKLPKDVYEAELLRLQAELVNMQEWVRTTGARVVVIFE
                     GRDAAGKGGAIKRVTEYLNPRIAQVVALPAPTERQRTEWYFQRYVQHLPSAGQIKLFD
                     RSWYNRAGVERVMGYCTPEEHRRFLRQCPIFERMLIEDGIILRKYWFSVSDREQEKRF
                     KSRLTDPMRQWKLSPTDLESLTRWEDYSRAKDEMFVHTDIPEARWNVVESEDKKRARL
                     NMIADLLNAIPYERVHNDQLKLPKRPPSTGYRRTDRGLQLEVPDWAATLIDKEPPPKY
                     VDPEDED"
     gene            complement(8543..11062)
                     /locus_tag="PKJ61_00055"
     CDS             complement(8543..11062)
                     /locus_tag="PKJ61_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016668326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3516 domain-containing protein"
                     /protein_id="HOC12255.1"
                     /translation="MRLTDLIPEAGPTDPDALYSAFIDAAAADGITLYPHQDEAIVDI
                     LTGANTIITTPTGSGKTLIATAAHFAALAGGGRSYYTAPIKALVSEKFFALCDIFGAE
                     RVGMVTGDAAVNAEAPVICCTAEILANIALREGRAADVAQVVIDEFHFIADRDRGWAW
                     QVGLVELPQAQFVIMSATLGDVSQLARDLSARTGRGTSVISDAVRPVPLTYSWSLRPL
                     QETVELLLRDAQAPVYIVHATQAAAVERAQALLSSGIIDRSHRRELVEAMQGFRFGSG
                     FGKTLAKLLNSGIAVHHAGMLPRYRRLVETLAQRGLLSIICGTDTLGVGINVPIRTVL
                     FASLSKFDGRRQRLLRAREFHQIAGRAGRPGFDTVGYVAAQAPDHVIENAVALAKAGN
                     DPKKQRKVQRKKAPEGFVNFTEDTFTKLIAAPPEPLVARLRISHALLLNLLQRDQDTG
                     VAVWSLICATTSDPGTRRALVRRAVAIGRSLLAAGVVVRLPEPTPGGRRHQLAVDLQH
                     DFALNQPLSAFALAALELLDLESPSYALDAVSVIEATLEDPRAVLVQQQFKARGEAVA
                     ELKADGVEFAERQELLEEVTWPRPLAELLEHAYEVFLGHHPWLAETPVSPKSVVRDMF
                     ERAMTFTEYVAFYRVQRSEGLLLRYLSDAFRALRQTVPEAARTEELEDLIEWLGEVTR
                     LTDSSLLDEWEQLTTLSGARPLEVAPPPQRQVTGNLRAFRVLVRNAMFRRVELAARDD
                     VDGLLELDSADPDRAGGFRDWDDALGDYYDEHDEIITDADARGPAMLIIEDAGRTWQV
                     RQLIADPEGNHDWSITATIDLDACDEAGELIVHVTGFSRLG"
     assembly_gap    11234..11333
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(11470..12105)
                     /locus_tag="PKJ61_00060"
     CDS             complement(11470..12105)
                     /locus_tag="PKJ61_00060"
                     /inference="COORDINATES: protein motif:HMM:NF036520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOC12256.1"
                     /translation="MANELPWERLPLRRVPTQKRSRDKVSRALVAADALVREEGPEAI
                     NLPRVAERAGVSVGALYQYLPDRESIVGALVWRYHARLEALLEGAVASARREPPSGDP
                     VEYALESVVEIYREEEAARPLRSFGSSPELQAQRQAHRARMASKVAELMQAAGTRPED
                     RDTTSIMAQVAFVAADAVIQDTFNYPEDERPERLRELGRLLHSYLKLIPRA"
     assembly_gap    12415..12514
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            12806..15343
                     /locus_tag="PKJ61_00065"
     CDS             12806..15343
                     /locus_tag="PKJ61_00065"
                     /inference="COORDINATES: protein motif:HMM:NF012906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HOC12257.1"
                     /translation="MNLADAEWTVRAEPSSDPFDEVPSDLRAALSDGIPATVPGCVHT
                     DLMAAGLLRDPYLGTAEGEQHWIGRQTWTYTGRFDLEEDDPLLEADYCDLVCDGLDTV
                     ADVYCNGHNLGHSENMHRRNLFDAKSALVAGANVIEIVFAPALEHAERMAALVGERIH
                     VERDPYNLIRKMACNFGWDWGPRLITAGIWKDIELRGWDTAKVLDVSVQATASQADDG
                     TWIGHLEVNAQLVGKIRFAALTVKLTGNDLEATVGVNAEDWTRATLDITGIQPWWPHS
                     LGDQPLYDLEILLHNDVAVLDRVHRRTGFRGTALDTTADADGTGAKFALVINGTEVFV
                     RGANWIPDDCFPTRVTPQRYRERIQQAKDANIDLLRIWGGGIYETKAFYDACDELGVM
                     VWQDFTLACAAYPEEEPFASEFEAEARDNIMRLSAHPSLVIWNGNNENIWGRDEWDWA
                     NQPGGDLTWGAGYYYEMFPRILAELDPSRPYWHGSPSSGSPEIRPNDPNFGCIHIWDV
                     WNERDYTGYDDYSPRFVSEFGYQGPATYATWARTLDVADRGATTSAMLVHQKAAGGNG
                     KLNAGIAGHLPQPGESVADFDEWLYLMQLQQANAVRYGIDRWRSLRGRCHGSIVWQLN
                     DCWPVTSWAALDLGTNAAGVPVARRKPLWYALRSAYADRLLTFQFEEGQWRLAVVNDG
                     TDAWLVDGTVQLRRLTGEIVWEKRFSREVPARARVDVTVTDPVPLESGLALVATADGA
                     ERCVRLLLPDVNADLPTPEFEARAVATADGAAITVTAKSFVRALCLFPDRVDEDAWAD
                     SALIDLFPGESHQFNVRGTFTASELARLTDAPVLRALDPHPTPSEAP"
     gene            15458..17281
                     /locus_tag="PKJ61_00070"
     CDS             15458..17281
                     /locus_tag="PKJ61_00070"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOC12258.1"
                     /translation="MSESIFEKPSRRSFLIAGGVVAGGIALAACSSGGAASPTSGGAT
                     SGSAAPAEKLGIGNNGKFGAGRKGNTADALLIAGFQWGPPATFNPLSPTAAWPAAANV
                     MQIVYESLLRWNIATGEIMPGLAKEYKVDGTTSITLTMQDGVTFSDGSPCTAKDVVMS
                     YELGKKNTGITVASFWQLADSIEATDDKTVVVKVNQKTKNVGNVLRMLTETYILPSAV
                     FSGIADDKLPTEVMDKPIGTGPFTLEKADQTQVVLTRNDKYWGQTFYGGLPVMKQIIH
                     PIFKSNEDGNIKFQAGELDVMQQFVSQIWKMWEGGKPVGTYLKEKPYYVPGSMPMLLM
                     NTTKKGLDNPAVRKAIASAIDYASIAETAMSGYSAEVKASLILPEGAESKFFNEADAT
                     ANGWKYDPAAAEKILTDAGAKKGSDGIYEIDGTKLGPYKLITPTGWTDWNASCEIIAK
                     SLKAIGIDCSTNFPQQAETTQAIQGGTFDMAVWYVSGVNPATPWARFKDIMSQAEMQP
                     LGKTTFANYGRWKNDEVEALLTEASQAVDDAAKKAAYDKLDALYRKEVPACPIMYRPD
                     EFYEYNATNFFNWPDEKNSYAPPMFRGAGNTWMYKIQKIAG"
     gene            17313..18320
                     /locus_tag="PKJ61_00075"
     CDS             17313..18320
                     /locus_tag="PKJ61_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020576071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOC12259.1"
                     /translation="MGLRRYLANKTVWYLGALLVAVVLNFLLPRVIPGNPVDVIVSRM
                     GQGGQSSEAQARVYEQFMREFGLDQPLWSQFVTYVTSAFQGNLGASFSFYPTPVAELI
                     AQSLPYTLVLQLPAIILGWIIGNVLGAVAAYKGGSWDKSVFTTSLLFSSMPYYCLSII
                     LLYTLAVVLGWFPPGGAYSLGMSPEWSWEFFSDALQFWWLPFISLLVINIGGQAVGMR
                     SMAIYELGSDYVNYSRSLGIGDRKMTTYIFNNAMLPQITGLALSIGTMVGGALITELV
                     FSYPGMGSLLFNAIASNDYPVVQAVTLIITVAVLVANYAVEILYGFIDPRIRASKSGE
                     K"
     gene            18322..19353
                     /locus_tag="PKJ61_00080"
     CDS             18322..19353
                     /locus_tag="PKJ61_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020576072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOC12260.1"
                     /translation="MTNIQSSAVAAETEGPLLPPEPSTPKAKAKPAWRSLNFGPRLWV
                     SAGLIVIIALLAFVGPLFYPFAPDEKVGSLYDGPSAEHILGTENFGYDVLAVLMASTR
                     NSLINGLLAGTLAVVIGVFIGILAGYIGGWLEELLMGMTNIVLAIPSIVILILISISL
                     QSTDIPFLKGIVGLALVIGITSWPWTARAVRAQASSVATREHIDVAKLSGARTGGILI
                     FDVLPYLASYVVMAWVLQLASAILAESTLSMLGLGPSGTNSLGMQLHWALAFQAVATG
                     AWWAFLPPTIVLTLISFALLYLQSSLDEVFNPRLRRGKKKHLAQAAARRAEAAKQLAE
                     QNQRAEVTA"
     gene            19350..20378
                     /locus_tag="PKJ61_00085"
     CDS             19350..20378
                     /locus_tag="PKJ61_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOC12261.1"
                     /translation="MSGILMRASNVTAVYAADTGKDVPAVDNVTLEVPDGQIVGLAGE
                     SGCGKSTLGNALAMIANPPLYVVAGKLEISDTEIDLSTLAKGANEVHRGHRGATVSLL
                     PQGAMNAISPTLRIRDLVVDVMRAHKRDTTKAEALDRATDRLKMLEMPTRVLDSYSHQ
                     LSGGMKQRMVTVISTLLNPQLLIADEPTSALDVSSQKALIEMLLAMVEAKIMRGVIFI
                     THDLPVLSMVTDRLAIMYAGKIVEDGPTEELVNNARHPYTSALLSSVLDPTEETRTKH
                     VLGIPGSPPNLGNPPSGCRFHPRCKFAMPACKLEEPAFLHEGEHEVACWWAMEHLNET
                     VPMEVNQA"
     gene            20375..21214
                     /locus_tag="PKJ61_00090"
     CDS             20375..21214
                     /locus_tag="PKJ61_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020576074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOC12262.1"
                     /translation="MSDPKSLDPGAIPPAGSPVLAASHVTKQFRVSGGHITAVDDVSV
                     EFHAGKVLAVVGESGSGKSTLARMLLRLMPVTSGTITYLGKDVTNIRGAELRAYWRDV
                     QAVFQDPFASFNQFFTVGHMLRRGMGAAGLSAETLDELIETCLGYVGLKPADAVHKYP
                     HQLSGGQRQRVMIARALMMNPKVLLADEATSMLDASLRVSALNVLQHLRENLGLTVLF
                     ITHDIGQACYLADEVVVMEHGLVVEQGTTDAVIFDPKNAYTKRLLADVPDLKGSLKSR
                     KLD"
     gene            21273..22649
                     /locus_tag="PKJ61_00095"
     CDS             21273..22649
                     /locus_tag="PKJ61_00095"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015070526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HOC12263.1"
                     /translation="MDIEFPSSFRFGSATSAYQIEGAATADGKEESIWDVFCRVPGAI
                     FNGEDGLVAVDHYHRYREDIALMRELNLGTYRFSVSWPRVLDAEGRVNAKGMDFYSRV
                     VDELLANDITPWLTLYHWDLPASLPGGWTNRNTAKRFVDYAQAVHAHLADRVRIWTTL
                     NEPWCSSFLSYAGGHHAPGQTEPVAAVRAAHHLLLAHGWTIQALRAADPDAELGITLN
                     FTPAIAASDESADVDIARRLNGTNNRYFTDPIFTGEYPADVLTDLASIWPDDLVHDGD
                     LAAIATPIDVLGVNYYTTNLVAAPAAGAEPPAGASPNITAPEAVVVDRGLPKTAMGWD
                     IDPSGLRDLLLWLHGTYTAAAGVSLVITENGAAFDDVADADGFVDDTADRLAYLKGHL
                     TAVHEAMEAGADVRGYLAWSLLDNFEWAFGYAKRFGIVRVDEDQRRTPKASARWFAEV
                     ARLGGFDT"
     gene            22892..23515
                     /locus_tag="PKJ61_00100"
     CDS             22892..23515
                     /locus_tag="PKJ61_00100"
                     /inference="COORDINATES: protein motif:HMM:NF017309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein LpqH"
                     /protein_id="HOC12264.1"
                     /translation="MSRPSFALLLALGLSSCAVVPPAGISPSGPASQPVPSVSPSTPL
                     GTPTPSATDVSPSATPSVSGSPTTTPSAPATALPAKGKGKLVLYQENLVSDQMTGTCQ
                     RKDGKPTLHLSDNATDFFESVEVKVVLTEAADAAVSVDMDFGADSESITHTLTAKGNG
                     QGGTSAKLSRTGETFKVTGTGTMTDDGKVSANAVPFAITVTCASVDW"
     gene            complement(23606..25780)
                     /locus_tag="PKJ61_00105"
     CDS             complement(23606..25780)
                     /locus_tag="PKJ61_00105"
                     /inference="COORDINATES: protein motif:HMM:NF013954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HOC12265.1"
                     /translation="MAISVAMSIGLALVDPATASAISANAKKTFVTSLATSAQAAQRQ
                     YGVPAAVLMAEAIVGSNWGTSAAAKQARNYFNTECRATMTASQFAKLAVAQVGKPYVL
                     GAEAAISQNNPAKFDCSELVEWLYGRSGNRITDLAASQYNVTKPVSIDAPQVGDLVFL
                     RNNPARSNGIGHVAVVTNQLDTGEWEIVEARGRADGVVKTTLSYWKQRKYFAGLRRYP
                     GFALADENGVTKGATANAYQYGCLAITVGGTTMRYSNYSSTSSAFADRARIITTGAKY
                     EAARGVLDNWDALVDEVAKADRPDTAAAYAQSLRDVINSYKLADYNVVPFTVVLGSGS
                     GTAKVTALKYLLRQSRVSVKVSGAYDAATKAAVKKFQKARKLESDGEAGPNTLTTLAP
                     QLKSGAKGDQVKALHALLQDFGYRTNSGSTFGSTTTAAVKAFQAAVGLSPSGVATSNT
                     WAKLFMAVDPAPVPTISGSAVVDGILTADPGVWAASATLSYQWFRDATAIPGANAATY
                     QVQAADIRSALTVAVTGSLAPFTPISRRSAATAVVVPGTFSAAPAPVISGTPAVGQEV
                     TAVTDGWAPAEVAFTYQWYRGKSTIKGATERTYVLQASDVGAKLTVKVVGSRAGYTSV
                     TQVSAELAGVAKGEFAAPEPKLSGKAKVGKKVTVSPGTWAAAKVSFKYQWYRGDSRIK
                     GATKSTYKITKSDKGKVLTVSVIGSATGFNSAEKRAATAKVS"
     gene            26006..27004
                     /locus_tag="PKJ61_00110"
     CDS             26006..27004
                     /locus_tag="PKJ61_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOC12266.1"
                     /translation="MSQPIRIGILGAARIAERALVRPAAAIGAEVVAVAARDLGRAQR
                     YASEHGIARAYGSYAELLADPEVEVVYNPLPNSQHAPMNQAVLRAGKHVLTEKPFASN
                     AVEAREVAAEPRDGLVLFEGFHHRYHPMYLRLLQVIGDGTIGELTELRVNMKFDCTDP
                     TDIRWSWPLSGGAQMDLGCYTTHVARDVAAVLGGGARPVSAVAGFREGIDPRVDASLL
                     VRAELPNGASAVLDADLGAAADMSITATGTRGTAVLPNFIAADADDRLILNVAGAETV
                     EHHGPTTTYTYQLQALTAAIRDGADFPTGADDAVANMEFVDECYRLAGLPPRESAQ"
     gene            complement(27087..27875)
                     /gene="cysE"
                     /locus_tag="PKJ61_00115"
     CDS             complement(27087..27875)
                     /gene="cysE"
                     /locus_tag="PKJ61_00115"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016454956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HOC12267.1"
                     /translation="MNPLRDEVWNRVRTEATADSLAEPALASFLHTTVLCHDRLEDAL
                     SYILASKLGSETISSLSLRELIIGAFDDDPSIGAAVREDLRAVLSRDPACRGYSVPLL
                     YFKGFHALQSYRVAHYYWTHGRSALALYLQSRISEVFAVDIHPGARIGKGIMFDHATS
                     VVIGETAVVEDDFSMLHEVTLGGTGKVGGDRHPKIGKGVMIGAGAKVLGNIRVGEGAK
                     IGAGSVVLEDVAPYTTVAGVPARPVSYPIHGLPALDEISDSIEP"
     gene            complement(27885..28316)
                     /locus_tag="PKJ61_00120"
     CDS             complement(27885..28316)
                     /locus_tag="PKJ61_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020579190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOC12268.1"
                     /translation="MTGLLVAAIAALVALGFGLYRKAIDGRVQVVGSPAAPTLDASRL
                     ATGLGSEATFVQFSAEVCAPCRATARVLAEVTAGLSGVEHIELDAATRLDLVNELGIT
                     RTPTVLLLDGTGAVRNRIVGAPRKTEVLAALEVLTESSDKR"
     gene            complement(28313..28762)
                     /locus_tag="PKJ61_00125"
     CDS             complement(28313..28762)
                     /locus_tag="PKJ61_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007078815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4395 domain-containing protein"
                     /protein_id="HOC12269.1"
                     /translation="MASQIDPRGPRFGATVTSVVLTLALVAGPKWGLALLVLQAFAFG
                     LGALLGLRYQPYGWVFRRFVRPRLGAPEELEDERPPRFAQSVGLGFALVGILGALFSS
                     DLVFFIAVGFALVAALLNAVFNFCLGCELYLLGRRLFARPAAASSKS"
     gene            29163..29636
                     /locus_tag="PKJ61_00130"
     CDS             29163..29636
                     /locus_tag="PKJ61_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC12270.1"
                     /translation="MSSVRQWLARPGLGSRAFAALATIAALWPVLSSLPRVSLVACFD
                     AAHPLYSLVPAEFGELHCLSAPAPVVAWTLMVGATLMVQLLFLPLVVTAGVVGGRALQ
                     RLARSARRQLTLVLVELGEIVVPQRRPAPVRVRAAYADVHHARVNPHRGPPSCLS"
     gene            29759..30538
                     /locus_tag="PKJ61_00135"
     CDS             29759..30538
                     /locus_tag="PKJ61_00135"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOC12271.1"
                     /translation="MSNRKNPQEQSNARTALRQQQAAQAKGEQRMKLATRIAWITGLL
                     VIALMIGVTVWSVTNSRTVTATPAPGGVVTPRNAAEGGGILVGDTAAPVAVTVYADFM
                     CPYCGQFEQVNGADLAAAVDAGSVKLEMHPMSFLDQQSGGTKFSTRAANAYATVANHD
                     AAIALDFMKILYDNQPEEGSTGLTDTQLADFAKQAGAPADVAASFTKQTFVPWVQQVT
                     ENAFASGIESTPTVKVNGVIQTENLLVPGALKAAIDKAANG"
     gene            30531..31151
                     /locus_tag="PKJ61_00140"
     CDS             30531..31151
                     /locus_tag="PKJ61_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HOC12272.1"
                     /translation="MADGPLRRLSTLRQSDGWIYTTMLVSALISLTASFVLSVDAIAL
                     AENPNAALACNINAVLSCGTVGVSWQASLFVFPNAFLGLIAEPVVITIAVASLGGVRF
                     PRWFMCSAQVVYTLGLVFAYWLFFQAYFVIGALCPWCLLVTLSTTLVFTSLTHVNIRD
                     GNLYLPPRANRAMQRFVRADLDALVVAIWLMAVVMLVMVKYGPMLV"
     gene            31179..31958
                     /locus_tag="PKJ61_00145"
     CDS             31179..31958
                     /locus_tag="PKJ61_00145"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HOC12273.1"
                     /translation="MSKQPKPSASSRRAALREQHAHEAANANRKRLLVRVGAVVAVVA
                     ALVAGLAVGAYVNNRPDDSASGPVAIGATGPANATPDGAVRIGKADAPVTVSVFTDFM
                     CPYCKTFDEANAEALSALLQSGKAKVDVYPLTFLDNQSQGSRYSTRAANAFATIANTS
                     PDQAWSFYQLLFAHQPAEGTTGLSDVQLAQLGEQAGASKEVVASFGKLAYRSWTDEVT
                     RQWFPKRVQGTPTIKLNGVVFDGDPYTAGPLAEAIQKVLGG"
     gene            32133..32759
                     /locus_tag="PKJ61_00150"
     CDS             32133..32759
                     /locus_tag="PKJ61_00150"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HOC12274.1"
                     /translation="MALASGILLAWGIWLPWLRGVRTLSVVAVLLALGVLGLSSFLAV
                     HGSVDTADDDADAVVVLGAAVHGRELSRTLVGRLDTALAYHQRNPRAVIVVSGGQGPQ
                     EEVPEAVAMRDYLVSRGVTADAVVLEDRSTSTEENFAFSKVLLDQRFDDDYRVVFVTD
                     EFHVYRAGCTAERAGLAAQHVSSRTPWYFWPANYLRETVAVVMDAFGG"
     gene            complement(33060..33455)
                     /locus_tag="PKJ61_00155"
     CDS             complement(33060..33455)
                     /locus_tag="PKJ61_00155"
                     /inference="COORDINATES: protein motif:HMM:NF024788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLD nuclease N-terminal domain-containing
                     protein"
                     /protein_id="HOC12275.1"
                     /translation="MIRVLPFAVELVLVIFCVLDCLRADESRIRSLPRWAWLLIMILI
                     PIVGCVAWLFAGRPTGSAPAQPPRPPSSGLYEQPPRQRVIAPDDDPEFLRSLKRSAEH
                     ERLLKQWEDDLKRREDELRKNQGQDPEQL"
     gene            complement(33569..34285)
                     /locus_tag="PKJ61_00160"
     CDS             complement(33569..34285)
                     /locus_tag="PKJ61_00160"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HOC12276.1"
                     /translation="MPRSMRATIIQPDPDVPADRFGRWLSGNRVLVRAIPLWQLGVPD
                     ADSLGDGLIVLGGRMSAHDGAQHAWMEPLKRLLREAVEAEIPTLAICLGHQLLAEAFG
                     GEVAVNHPGGGEHGAVEVDWHLAAADDPLLDRLAANGRSVVAASHHDAVTRLPEGSVS
                     LAASRDYPNQAFRVGSALGVQFHPEASPELMGRWAALDGGDARTMRRSMQTHDSEVAR
                     TGRLLAQAFTGHLRAQQLAA"
     gene            complement(34378..35700)
                     /locus_tag="PKJ61_00165"
     CDS             complement(34378..35700)
                     /locus_tag="PKJ61_00165"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOC12277.1"
                     /translation="MAVRQRPAPKSADGKSPDHTYPWSSVLFRLAIAENRLDRARLLL
                     LCAFALSGLLVFSWLARIPSVRDALDLSATELGGYLLVGSVGALITVFSASALLTRFG
                     SGRVFATGSIVMSAGFALMGIGPTLDSRPLFLAGIVINGIGGALINLPMNVESARLEQ
                     AYGRTIIPHFHAAFSAGAVAGSLLGAVASSVGLTVWLQFTIAAVGVGCARLAMLEHGM
                     ILPEARLTGPARDRGPSALGVWLQPRTLLIGAVAFAAALSEGAANNWLALAFVDGFGT
                     AESSGGLVLGAFIGSMTLVRAFGTGMIDRLGRTLSLQISGVLAFAGLAIFGLAPGPAT
                     AVAGVCLWGVGAALCFPIAVAAASDEPHVAAARVSVVASLGSVAAMTAAPLIGFAAAY
                     LGGARHALLVVLVALVVSLAVSRHVSPVATIEIADPMADFERTPVPVA"
     gene            35856..36455
                     /locus_tag="PKJ61_00170"
     CDS             35856..36455
                     /locus_tag="PKJ61_00170"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: protein motif:HMM:NF003768.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HOC12278.1"
                     /translation="MTLTAEAASISADSIFADMHTAYRFTSEPVNDEELARIYELAKF
                     TPTALNSQPLRITFVRTPEAKARLLPLLNPTNRPKSESAPVVAILAADTDFHEHLPVL
                     NPQDAGVRESLAENAERRAVMATNNAWLQAGGFILAVRAAGLDAGPMGGLNAAAVDAE
                     FFPGTALRTLLVVNIGHVAPDGNFPRNPRLGFDQAVTLA"
     gene            complement(36554..37240)
                     /locus_tag="PKJ61_00175"
     CDS             complement(36554..37240)
                     /locus_tag="PKJ61_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HOC12279.1"
                     /translation="MNLIAELWARATARSAQLEPTITLIIAGNALAAVLLAWPVVRLL
                     VTICHEGGHALVALLTGRQLRGIRVHSDTSGLTLTRGRPTGFGMTATLLAGYPASSVL
                     GLGAAWLVASGYAAGVLWLSVAGLAVMLLSIRNFYGALVVALTATLLALASWFIPGDF
                     VAWLAGLLTWTLLLAGPRPVIELLGNRSPNSDAAQLARLTHVPRAFWTLGWLALSIAA
                     LLVGGRWLLA"
     gene            complement(37371..38396)
                     /locus_tag="PKJ61_00180"
     CDS             complement(37371..38396)
                     /locus_tag="PKJ61_00180"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOC12280.1"
                     /translation="MGSRSFDKLRAGQARPADQRSERPIADLSEAATSPAIAVSGLTK
                     RFGDFTAVDNIDFEVPRGQVFGFLGPNGSGKTTTIRMLTGTLRSSSGNALVLGEDVIR
                     RPERVQPRIGYMSQKFSLFEDMTVDENLRFYAGVYDLSPQQFTAQRDYVLKMADLVGR
                     EDELTRNLSVGWRQRLALGAATIHEPELLFLDEPTSGVDPVARRQFWDLLYDLASRGV
                     TLFVTTHYMDEAAHCNRLAFIYRGRIIASGSPAELRTEHMDQRIFDVSTRDADPALLW
                     LEQSPEIAEAYLSGASLHAVVPSGSRLSAVELGEQLAVAGFAGATVAEVEPTIEDVFV
                     NLVSRQR"
     gene            complement(38410..39531)
                     /locus_tag="PKJ61_00185"
     CDS             complement(38410..39531)
                     /locus_tag="PKJ61_00185"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOC12281.1"
                     /translation="MSIKRIRLLIWKEFIQLRRDPMLIRLLLVMPVVQLIMFGYVVAV
                     DVHNLTTAVVDLDRSTTSQKLEAAFTGSGYFTVTQHPASEDDLRPLLDRGTVKVALVI
                     PEGTHDRLTRGETAPIGIIVDGSDSQISAIGSGYASQIVAQFNSARRDELGVTVAAPG
                     VDARIRVMFNPTLDPVNTMIPGLMAAIMMISLMVIMSQAVVKERESGTLEQMFVTPIK
                     ATEYLIGKLTPYAALATAQLLLVAAGGIFWFRVPFNGDFWVVLVGLLLFMFTCLGLGL
                     LISLVSRTRHQAQQAVMFLMIPTMVLSGFIFPIQSMPAVIQPLTNLIPLTFALEVLRG
                     AFVKGSSFEALAVPLLALAGFGVVIFSAAVIATRRRIAE"
     gene            complement(39528..40673)
                     /locus_tag="PKJ61_00190"
     CDS             complement(39528..40673)
                     /locus_tag="PKJ61_00190"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOC12282.1"
                     /translation="MKAALVRIGAIVRKEFRHLGRDSRTLLAVVITPVIQLLLFAYAI
                     SFDVEHVPTIVIDFDQTTQSREYASLYSSSPFFNVVQHGSSMSDVDAAFDANSARVAI
                     VIPSGFAKSLAAGEQAQVAVLIDGSEPNAAKVSQAYSVALNQLYSQELTRDWADAQGL
                     DLSSLGMLEPRVRTWYNPERRSSDFLIPGLMIVIIAIVTVQQTAVTLVRERDLGTQEQ
                     LEISPMRRVELMVGKLLPWTLIAFLDVALITGLGRTIFEVPLRGNFWTLAVGAALFVF
                     ASLGMGLLISAIAPSLDTANIIAMLVAFLPSFLLSGFAFALDQIPTWLAWISYAFPAR
                     YMVTISRGVFLKGAGFTEVWPELALLSGYALVMIVISSVLYGRRSSR"
     gene            complement(40670..41671)
                     /locus_tag="PKJ61_00195"
     CDS             complement(40670..41671)
                     /locus_tag="PKJ61_00195"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOC12283.1"
                     /translation="MSTTGPPADLGVIDDTLAVRIRGLRRTFGETVAIADIDLDVPAG
                     AVFGLLGPDGAGKSTLIRMLATVLTPDAGDAEVFGHSVTREAALVTPRIGYMSQRFSM
                     YPDLSVSENLNFFATIRGVSRADRRTRSKALLESMGLAEFTDRQAQFLSGGMKQKLML
                     ATTLMHEPDLLLLDEPTTGVDPVSRREFWRILAGLHRAGKSVVVATPYMDEAERCTQV
                     AFIDKGAIGESGTPEEIKQRVPGQLVEVVAAEPRAALAALQDVPGVASPHLFGDVVRV
                     LWSDAVAPVQPLAAALAEAAIADVAVRPIPMDMESVFAFLADTRAPAAAGSLEGGES"
     gene            complement(41671..42462)
                     /locus_tag="PKJ61_00200"
     CDS             complement(41671..42462)
                     /locus_tag="PKJ61_00200"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOC12284.1"
                     /translation="MAAHTRKHPPIPVIVVVLLALIGGGIWWWWSSTQPAASAEAQLS
                     GAVEANTYQVASALSGRVIEVKVAEGDRVAKDDELVRLDDSAFKLQLEQAKQGVVAAK
                     AAVTNAKDDDDATKADVKAAEARLAQAEAAVKLAQVQLGYTVVTAPRAGTVTSLTTNA
                     GQNAAPGKTLLTLVDPADLFVRVFVPETQIGRVLIGQAATVTTDSSADSYSGTVSFIA
                     SESEFTPNNVQTKEQRVKLVYEVRVRLNDASGTLKAGMPVDVAFA"
     gene            complement(42465..43706)
                     /locus_tag="PKJ61_00205"
     CDS             complement(42465..43706)
                     /locus_tag="PKJ61_00205"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HOC12285.1"
                     /translation="MKRFAAVLVGTLLVAGCAPAPAELVVAGRVKDRLETVAVPTLAM
                     PAVNLDAGFESSGQRTADASRTASTYALGSFIQLADVLVAEGDTVRVGQTLATLDASG
                     LEAQVQVAKADAKVTAAQVDLLAAAIDKTDDKAEEVADKTADVKKAINTLTDTKAKLV
                     KARKQLKKQRPLVAKQLKQAQELLANYPPVPVPGIPSREELQQGIAKLKAALKKMDAG
                     LKKINTNLPKLEKGLRKAKDGLEKLADAADKITDARAGLVDLKELAEIAADTMQVPIE
                     LAQVQFKLAVLTAPANGVVVSVAASGDRLAPGATAVAIRPATPSKVTAWLSPVQLGQV
                     CVGDEASVAGDWGNAATPATLTRIAPAAEYPPTSVPTEEVHLTRAVEVEFTATAQLPA
                     GVPVEITINGCRFAADHEEGR"
     gene            complement(43703..44320)
                     /locus_tag="PKJ61_00210"
     CDS             complement(43703..44320)
                     /locus_tag="PKJ61_00210"
                     /inference="COORDINATES: protein motif:HMM:NF025605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOC12286.1"
                     /translation="MTTALSPRRAAKRAQISAAARKLFLAQGFAATSMDAVTAEAAVS
                     KQTLYAYFPTKIDLLGDVILNGISQLVMDPPDLDRMRTLEDLRRNLVEFSVRLTANLL
                     QPESIALVRLVLGEVFRVPELRSGFREALPGQVLDRTEKLLRYSAELDLIRLDDPELS
                     ARMYVGSMMTYIALDGFLSVEPSGPPPRAELEKLVDVFLTTVTRS"
     gene            44389..45804
                     /locus_tag="PKJ61_00215"
     CDS             44389..45804
                     /locus_tag="PKJ61_00215"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HOC12287.1"
                     /translation="MTNPVAELARRRNDLPGPVDHSLPLPKRLLKSGWTWAIVALTLA
                     YTAALFSQYFTITADVPVTGGVIPGINSAAIRRAAWLALPTLAFWTVVFIWADRYRPQ
                     RLAVWFLTLGWGGSIATFVSLHINTWAAQQMAVTGNGDPATSARAAIFIAPFVEEAAK
                     ASVLFLIAVIARYRLVSKVSVIVLAGLSAAGFAFTENIIYYARAIVYSSENIGVGDAD
                     AAVNQIVWLRGFWTCFGHPLFTMMTGIGVAVALRTRSKVVRVLAPVIGYGAAALLHMI
                     FNSQASLNDQNGQMFVYAFVALPMVLSAVIYVVRQVFKQGRVLALRLDDYVRLGWLPA
                     SDPMVFAKLRTRLWALLVAITRGWRTFTATLHLQRTLTELAYLRDGQIRGVYDEAGNV
                     RARELVETAQSLRGIAIADPRGLRLNLPRIRRPRAVGYVPPAYPGPSGIGGNWPARPG
                     AAPLGSPGYSAVDPKWGPPPG"
     gene            45814..47715
                     /locus_tag="PKJ61_00220"
     CDS             45814..47715
                     /locus_tag="PKJ61_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
                     /protein_id="HOC12288.1"
                     /translation="MSAGRLAASLVRLRDALAGVRLPLELPGSADALARARGYVAQLD
                     DYVLPRLDRLDAPLLAVIGGSTGAGKSTLVNSLLGRVVTAPGVIRPTTRSPVLVHHPD
                     DAEWFASDKILPGLARSTRTASDARTLQVVAEPTLPRGLAILDAPDVDSVVAENRALA
                     AQLLAAADLWLFVTTAARYADAVPWDYLRTAAERHAVVAVVLDRVPPAAMGEVPAHLG
                     QMMGQRGLGSSPLFAVPETDVDATGLLPNTAVAPIRDWLGELAADAVSRNRVVLQTLD
                     GAIGALLAGAPGIAAAAEAQAASLDTLRSDAEKSFAEAVRTISVQTADGTLLRGEVLA
                     RWHDFVGTGEFFRAVEQKIGWLRDRLVAAIKGEPKQALDMKAAVESGMEVLIREEGEA
                     AARRVESAWQAHPAGRELLAATKEDLSRASRDYPLAAANAVRSWQGDVLELVSREGAA
                     KKSAARFWALGVNGVGVALMVLVFSQTGGLIGAEVGVAGGTAVLAQRVLEAIFGEDAV
                     RRLAKQAKTDLDARVEAVLAAELSRYLAAVDGVDAAPDQAEQLLAAVAEVDATRAADG
                     RLTLPGELALSDQPVSPARLVERAQTPSLLDRSTTDQPVEHVALPNPSATDQDIAITA
                     EVLASEDEQ"
     gene            47712..49313
                     /locus_tag="PKJ61_00225"
     CDS             47712..49313
                     /locus_tag="PKJ61_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HOC12289.1"
                     /translation="MSAADDHLARRVRALGEAVDAASGRSDAQLVAKAKAIVARAGER
                     VALSGSYTVVAFAGATGSGKSSLFNAITETELARVAVRRPTTSKPLAVSWGTELPHEL
                     LDWLDVTKRHLVPSGPSEFSDLVLLDLPDHDSTELGHRLTVDRLVELVDALIWVVDPQ
                     KYADAALHDGYLKRLAPYADVMMVVLNQADRLPPAELDRCLSDLRRLLDSEGLRATPI
                     MATSALRGTGVAELRSALARTVANKQAMTKRLAADVSVTAGELAAELGASAEPDINNR
                     VRRQLVSVLGDAAGVPVVVDGVRRAWRKRGAAATGWPFVTWIARLKPDPLRKLRLDLP
                     KGEHSPTEVNRTSLPKATSVQRARVDQALRELVDVASDGLPRGWMLAVKGAVQGNEEK
                     LLDDLDRAIAGADLTVRRGTWWWALFGVLQWLLIAAVVAGAAWLFSGPVLASMGLPPL
                     PTVLWYSIPAGTWLLVGGVLGGILLAVLGRAFVEMGANSHARTAQRSLDEAVADVVAS
                     EVLVPVESELARYATARKALRTAMG"
     gene            49431..49904
                     /locus_tag="PKJ61_00230"
     CDS             49431..49904
                     /locus_tag="PKJ61_00230"
                     /inference="COORDINATES: protein motif:HMM:NF012651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOC12290.1"
                     /translation="MDAVIWMTGYVGSEVEYRQVREDLVFASFRLACTPRLRRGGEWS
                     DGETTWIGVTCSRALAENVRDSIAKGDPAVVVGKLRTSRWKDDQGVSRERLVLEALAV
                     GHDLNRGVTEFTKNVRPPVEEVQVNLGEMVMATELQEEVWSAEADSQADGVAAVA"
     gene            50010..50705
                     /locus_tag="PKJ61_00235"
     CDS             50010..50705
                     /locus_tag="PKJ61_00235"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOC12291.1"
                     /translation="MGRKGSYAKGIAKREEILTSALEVIAREGYGHASLRELSDAVGL
                     TQAGVLHYFDSKEDLFVEVLRRSDQLAWARVGPPQGAETAERFLANLAGNTKEPGLTQ
                     LYVQLSAIAAAGEGPARTYLASRNRDFHRWVAESLRLEREADPSFASGLEPLLATRVI
                     MAVSDGLQNQWLMDPSFDLAAIVGHVIELLVRGGAVVDEARDVPEDHSRALVVPAVAR
                     LTAPRPRPNTIPA"
     gene            complement(50794..52476)
                     /gene="ettA"
                     /locus_tag="PKJ61_00240"
     CDS             complement(50794..52476)
                     /gene="ettA"
                     /locus_tag="PKJ61_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HOC12292.1"
                     /translation="MPEFVFTMHNVRKKLGEKLVLDNVTLSFFEGAKIGVVGPNGAGK
                     STMLKLMAGLIHADNGDAMLGKGKTVGILQQEPPLTEGKTVLENIHEGVAETKGMLDR
                     FNEISLAMAEPDADFDALLAEMGELQTELDHRNAWDLDSQLDQAMDALQCPPPDAIVD
                     VLSGGERRRVALCKLLLQQPDLLLLDEPTNHLDAESVNWLEGHLKTYPGAVLAVTHDR
                     YFLDNVAEWICEIDRGQLHPYEGNYSTYLETKKARLQIEGKKDAKRAKILERELEWVR
                     SNPKARQAKSRARLARYEELAAEADRNRAVDLAEINIPPGPRLGSDVLEAKKLTKGFG
                     DRVLIEDLSFTLPRAGIVGIIGPNGVGKTTLFKMLIGEEQPDSGELKVGSTVKFSYVD
                     QGRSGIDAGKNVWEVVSDGLDHIKVASFEMPSRAYVASFGFKGPDQQKLAGVLSGGER
                     NRLNLALTLKMGGNVLLLDEPTNDLDVETLQSLEDALLEFPGCAVVISHDRWFLDRVA
                     THILAWEGTDEDDAVWFWYEGNFADYEENKVARLGVEAARPHRTAHRKLTRG"
     gene            complement(52544..55339)
                     /locus_tag="PKJ61_00245"
     CDS             complement(52544..55339)
                     /locus_tag="PKJ61_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015071001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HOC12293.1"
                     /translation="MPDAAGFTEQVSALAGAGWPVDSRTLTRALYSSDASLYRVPPAA
                     VSRPASVPELVDVVDAARAAGLPVTTRGAGTSIAGNAVGTGLILDSTRLSRIAHLDPI
                     TKAAIVEPGVIPARLTAAAARHGLRFGPDPSTSDRCTIGGMIGNNACGPRALGYGRTA
                     DNVLGLDVITGTGERLSLASGTVAHANSPALSALHELVQANLGIIRTQFGRFPRQVSG
                     YSLEHLLPENGFDVGRFLVGSEGTLAVVTGARLRLVEDLPHRVLVVLGYPDMALAADA
                     TPVVLEFRPTAVEGFDRRILDVVGRLRGSSAVPTLAAGDGWLFVELTDADADRLAERA
                     GKLVAAAGALAAQLIFDPDEAARLWRIRADGAGLASVGLARPAHSGWEDAAVPPAVLG
                     AYLRDFDRLLAAHDLHGLPYGHFGEGCVHVRIDFNLAGATGVTGYRAFVEEAAELAAG
                     YGGSCSGEHGDGRARSELLPKMYSTEALALFAAVKQIFDPDNLLNPGVLVDPRPMDAD
                     LRLPAAAGSRLSGLDRTFVADVHRCTGVGKCLADTGGVMCPSYQATGREQDSTRGRAH
                     ALQEMVNGTLVTGGWRAPEVHEALELCLSCKACATECPTGTDVAAYKSRVLAEAYRHR
                     LRPRSHYALGRLPRWGRLVTAVPGLAAVVNAALAAPVLGTAAKWLAGVDSRRLLPKFA
                     DRRARSRPDPLVSGRRAVVWVDSFTDTLDATRLDAMLAVLVGAGFAPTILRRTACCGL
                     PWITTGQRAEAARLLSHAVELLHPIVAAGTPIVGLDPSCVAVWRSDAADLVVDVRLAE
                     VAAGIHTLAELLTTLPEWSPPDLHGTVVVAQPHCHHSSVLGWDADAALLARTGAQVVT
                     VSGCCGLAGNFGVEIGHYETSAAVAQNHLLPAVAAAGPDAVLLADGFSCRLQLADLAG
                     RRALTLAELLTLPAAHR"
     gene            complement(55344..55802)
                     /locus_tag="PKJ61_00250"
     CDS             complement(55344..55802)
                     /locus_tag="PKJ61_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013160716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOC12294.1"
                     /translation="MTTQLQPRWLSTEQQQIWRTYLNGVAAISETLEAELRPFGLDLG
                     EYEILVHLSESPDHALRMSDLAERVRQSRSRLTHTISRMEAKGIVSRTACPSDRRGVI
                     SVLTDAGHQLLVAAAPHHVESVRRALVDPVDPADFAALGRAMQAVLDVTD"
     gene            56077..57909
                     /locus_tag="PKJ61_00255"
     CDS             56077..57909
                     /locus_tag="PKJ61_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HOC12295.1"
                     /translation="MIDVDAIVSAAPPTVGAMFRWRVAKTPGKEAFRYPDAKDNWVSI
                     DWAETGRQVFDLAAGLLSLGLQLEERVAIVANTRIEWILADFAINAAGGATTTVYPNT
                     QGADFAHIVTDSGSVILVAENAEQVAKLESAGDAAAQLRHIILMDGEGDGERTLGWSQ
                     LAERGRQLLASDPGVVDRAIESATHDGLATLIYTSGTTGMPKGVELSHRCWVYQAFVL
                     DAMEIIADTAVQYLWLPLSHVFGKDLLTIQLAIGFSSAVDGRVDHIVSGLGEVRPTFM
                     CGAPRIFEKVRAAVMLASPRGGIKGKIARWAFAVGRASREYRLADKPLPPAMRLAYTV
                     ADHLVFSKLKDRVGGNIDFFISGSAKLNAQVQAWFYSAGLVVAEGYGLTETGAVSCVN
                     LPLPPRFGTVGPALPGTQIRIADDGEVLIKGPAVMRGYHNDPERTAEVLVDGWFHTGD
                     IGKLDADGFLSITDRKKDLMKTSGGKYVAPAKVEGVIAATIPYVSQVVAVGDGRKYIS
                     ALITMDRDNLAKWAARKGLTELSYEELTQSDELRRTIERFIHSANAKLERWETVKRFA
                     ILPAEFSVDDGGVTPNMKIRRKVVTEQYADVVASLYEDDAVDEG"
     gene            57906..>58267
                     /locus_tag="PKJ61_00260"
     CDS             57906..>58267
                     /locus_tag="PKJ61_00260"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HOC12296.1"
                     /translation="MSSFRSRVPLRWVDLDAQGHVNNAVIADYLQEARVEWLLAGPNA
                     HLLGSATMVVSHQVEYLGPVLFSLAPVEVVLRVGKVGASRFSLGYSVQQDGREVARAN
                     SVLCLFDYAAGRPRRMTSA"
     assembly_gap    58268..58367
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <58368..58602
                     /locus_tag="PKJ61_00265"
     CDS             <58368..58602
                     /locus_tag="PKJ61_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC12297.1"
                     /translation="YLGQIAHRGEPYLVRTGVCRVGRTSVTIAAQVEDPRAVGVLART
                     LTVLVAGDEHGRPVPLPASMHAAAERWPAEPAP"
     gene            58602..59150
                     /locus_tag="PKJ61_00270"
     CDS             58602..59150
                     /locus_tag="PKJ61_00270"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOC12298.1"
                     /translation="MVGGQRVEVAFRTPRRDELAIREQWLADPGFMAYNAGWAVDFPG
                     YHPDTGCVDFPPDAWDGWYDYWIGRGERDYWFVQDSSGQLVGHAHYRVEPAADGRRVA
                     HLGASVHPDHRGRGLGYATFAELVRRIEAAGVADVARNELDAGRVAAIRIHRALGFRP
                     GSVSDELDPARPVTVWELPLQS"
     gene            59313..59933
                     /locus_tag="PKJ61_00275"
     CDS             59313..59933
                     /locus_tag="PKJ61_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012923207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC12299.1"
                     /translation="MRARPGRFGRRLSQLLVGLVLYGVSMAMLVRSGLGVDPWDVFHQ
                     GLANHVPLSFGQVTIAVGALVLLLWIPLRQRPGLGTLLNVIVIGLAADAGLALIPIAE
                     SWLAGTGLMLGGVAANGLAGALYVGARLGPGPRDGLWVALTQRTGHSIRLVRTVLEVT
                     VVAVGWLLGGVVGIGTVVYALAIGPLVQLFMPWVEVRLDEPERNDG"
     gene            complement(60098..61093)
                     /locus_tag="PKJ61_00280"
     CDS             complement(60098..61093)
                     /locus_tag="PKJ61_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020574287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOC12300.1"
                     /translation="MKRQRASVKDVAALAGVSVGTVSNVMNRPESVTEKTRTKVEQAM
                     AELNFHRNASARQLRAGVSRTVGAIVMDLGNPYYTELARGIEDRLAQDDHTLILCSSD
                     EDPEREGRFLRLLAEQGVRGVLVTPMSTTKERLAPLRQLGIASVLMDASSRDHAAVGV
                     DHVSGGRQAIGHLLAQGHRRIAYLAGRDELQQTRHRLKGAVQGIQNAGLEPAKVLQVI
                     HLSNLNAADGEEGMGRILDDGAKPTAVFCMNDIVALGAMRAIRARGLRIPDDLAVVGY
                     DDLYFAAELMTPLTSVRQPMRQLGWAAADLLLTEHPHSLFEPELVLRASSDFKRG"
     gene            complement(61193..62461)
                     /locus_tag="PKJ61_00285"
     CDS             complement(61193..62461)
                     /locus_tag="PKJ61_00285"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016667904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HOC12301.1"
                     /translation="MSLLITGATLADGTRGDLYVADGRLADPASAPAGVERIDADGLL
                     ALPGLVDLHTHLRQPGREDAETVASGTAAAARGGYTCVHAMANTNPVTDTAEAAEYIH
                     SLGGSDGHCEVRVVGAVTKGLAGEELAELGLMHASAAGVTVFSDDGRCVDDSLLMRRA
                     LSYVKAFDGVVAQHSQDPRLAGPGACCHESEWSGRLGLPGWPAQAESVIVARDVQLAE
                     LTDSRVHVCHVSTAESVDVVRWAKKRGIKVTAEATPHHLLLDTPLVAGYDTTFKVNPP
                     LRTSEHLAAVREALADGTIDIVGTDHAPHARQDKDHPFDVALPGMLGLEQALAVVIDV
                     MVNSGRMDWPTLVERMSHAPARIGKVAGQGRPLAVGEPANLVLVDPSARATVDRDASR
                     SRARNNPYHGLDLPDPVVATIWNGRVTYQR"
     gene            complement(62458..63408)
                     /locus_tag="PKJ61_00290"
     CDS             complement(62458..63408)
                     /locus_tag="PKJ61_00290"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020390674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HOC12302.1"
                     /translation="MRHLLSAADLTLAEATSILDVAAEMADVQRRPVKKLPTLRGRTI
                     VNIFFEDSTRTRTSFEIAGKWLSADTINVSAKGSSVSKGESLRDTMLTLDAMGVDCFV
                     IRHASSGAAAQVASWVRAQVVNAGDGQHEHPTQALLDAYAMRAHFRKIGSTDTSGGFA
                     GKKIAIVGDLLHSRVVRSNVLLQRTLGGEVTLVAPPTLLPTGVETWGCPVTSDFDEAL
                     ADADIVMMLRVQRERMAGGFFPTEREYAEAYGLTEERLAALKPGAAICHPGPMNRGVE
                     ISSAAADAANSLVLEQVAAGVAVRMSVLYHLLGSNEGAMK"
     gene            complement(63405..63974)
                     /gene="pyrR"
                     /locus_tag="PKJ61_00295"
     CDS             complement(63405..63974)
                     /gene="pyrR"
                     /locus_tag="PKJ61_00295"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008356786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HOC12303.1"
                     /translation="MAEATNQPAEASRVVADAPELSRSLTRMAHQILEANQGCEDLVL
                     LGIPTRGVHLAKRLAKLIEAIEDRPVRLGVLDITMYRDDLRANPTRRVGRTSIPGRID
                     DTVVVLVDDVLYSGRTVSAAIDALRDLGRPRAVRLAVLVDRGHRELPIAADHVGRAMQ
                     TAADERVQVLLDELDGRDEVVISRPGGTR"
     gene            64150..64320
                     /locus_tag="PKJ61_00300"
     CDS             64150..64320
                     /locus_tag="PKJ61_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC12304.1"
                     /translation="MPLPALVPLETLPGWPAVQEPTAIELLTLTLFIPLAITAVVMLL
                     TMARGWKSPDRG"
     gene            complement(64458..67091)
                     /gene="pepN"
                     /locus_tag="PKJ61_00305"
     CDS             complement(64458..67091)
                     /gene="pepN"
                     /locus_tag="PKJ61_00305"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002516958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HOC12305.1"
                     /translation="MYPANITRAEAAERSNQIAAHSYDITIDLSGRVPDAEPFDPTAT
                     FVSTTTARFSTTGGDAYLNLIADRVLAATLDGAPLSVEAFVDHKVWFAASAGEHEVTV
                     SALCRYSRTGEGLHRFVDPVDDRVYCYSQFETADARRAFACFEQPDLKATFAFTVIAP
                     ADWTVISNSAAVEPVIEGQEGLDQQNGEGLDQPSALARWTFAPTPPISTYITAIIAGP
                     FHTVTDSYAGREKDIPLSVSCRQSLVPYLDAERILSTTKAGFAVFEEHFGMPYPFADY
                     AQIFVPEFNAGAMENAGCVTIRDEYLYRSRVTNASYEARDNTILHELAHMWFGDLVTM
                     TWWDDLWLNESFAEWASHFCQAEIRAKGSAGDNPWATFANSRKTWAYRQDQLPSTHPV
                     AADMVDLAAVELNFDGITYAKGASVLRQLVAFVGQEAFLAGVRAYFAEHAWGNTQLSD
                     LLAALTTASGRDLSHFAAEWLQTSGVNTLRAEFETDADGNFSRFAVRQTAVPQWPTLR
                     HHRLAIGLFAEADGKLARTHRVEVDIAGELTEIPELIGQARPDLILLNDDDLTYAKIR
                     LDERSLATLVSGIGRLDDALARALCWGAAWDMCRDGEFRASDYVSLVLSGAGVEDDLT
                     AVQALMGQSRTAIDYYAPRDTRAELNDRLIAGLARLIKDAEPGSDHQLAFARALIQAA
                     DSDAVADLLKAWLNGDEAPAGLAIDTDLRWLIVTNLARLGVFGEAEISAEAERDATAA
                     GAEKAAGARAAQPLAEAKTEAWRLITADPDVANETHYQISQNFWKFGQDEVLRPYVQR
                     YLEVAEAISDGRDGWGERSSAIRQHVLGLLFPSPLADRPTLDGIDQWRANTVLTDAVA
                     RQFAEKRDDAERALFCQAAGA"
     gene            complement(67180..67368)
                     /locus_tag="PKJ61_00310"
     CDS             complement(67180..67368)
                     /locus_tag="PKJ61_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC12306.1"
                     /translation="MTTPARHPEPDRGFGRDGVSGLVDPARAMRARDVSRPTAADLAA
                     AEQTAEALLAAPGQRSPH"
     gene            67416..68228
                     /locus_tag="PKJ61_00315"
     CDS             67416..68228
                     /locus_tag="PKJ61_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014167025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HOC12307.1"
                     /translation="MPEGHVIHRLAGELNAAFAGRGVRVTSPQGRFAGAAELLDGRPL
                     RGAEAFGKNLFLVFGEDAVVQIHLGLIGKLRWVGPGVVTNPDTVRLRIETEADAVELR
                     GPQTCALIGSVEMDAAVAALGPDPLRPDADPDRGWQRVHRSARSIASLLMDQRVAAGV
                     GNIYRAEVLFRARLHPETPGNELSPRKWRELWADLVELMPFGVRDGRIDTVRDEHSPE
                     ATGREPRVDRHGGEVYVYRRAGLPCLVCGRAVSVREFEGRNLYWCGKCQRRR"
     gene            68210..69658
                     /locus_tag="PKJ61_00320"
     CDS             68210..69658
                     /locus_tag="PKJ61_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC12308.1"
                     /translation="MPTAAVMAWGEAEVRLAADGTSARLFANGDLVVEVGWVDAVGAD
                     PELELADEVEHTLQIPGGRLLQRQSVADAWRSRWVLLPASDVALTATPPRVRVRPGPE
                     YTLWSWGSGVTALLAVSPAHAGGPVLGFRLEQGYLEEAGDPEWSSATAEYLVAPRGSE
                     LSSGQRLVTSLRANWFDHLDDLAAKLPPWLNDTQLDFEEEWWGDLADFGVTGPDDVQI
                     AYAEGMVVIAGEGGRRAIDVQTPRGLSRVPVEWVPSVQDVLHAVAKTKLSLSRPLSAA
                     EAFCVQLAADQHLLRLTDAHEDLLDQVDWAAEDSVIGAAFALLRGRKLGEAALVSDAL
                     RLLARLPVGVGYGRTVMAGWLASISLGLDARERCLELLGRSAVGRTASLESSLLHYRN
                     AEFGAAELDGVANRLGGTLPGEAPLLTWTEQAELVGLLELCPPEWPGAARYSLVAEKA
                     RGQVLCGYYDERITDAEPLAMLLLSPELRAGG"
BASE COUNT        11112 a        22911 c        24060 g        11403 t
ORIGIN      
        1 tgccagggtg cagtcactcg cccgctttcg cagcgacttc gaggagtcat tggccacagg
       61 ctgggccggc ttcgctgggc ggctgatcga gagggcggcg ccacgtcccg ccggcgtacc
      121 caccgcctat gtcatgcaac ccttcgaggt ccgactggac tatctaggac gcccgtggtg
      181 ctcggtgaag ttcgagctcg ggcatgacga gatcggtgac gccgacgagc cggagtacca
      241 gctcgcgtcc gacctcgcgg ccctcctcac cgaggtcgga cttgagccac ccaagccggt
      301 agcggtgatg cgagccgacc atcagatcgc acagaagcta cacgccgcct cagaagcagg
      361 cagcgagcgg gcccgggacc ttgtcgacct ccaactcctc gatcgcggcg agaaactgga
      421 cctggcccag gtcgcctcaa cctgtacgcg cttgttcgac tatcgacagc agcagcaatg
      481 gcccccgatc atcgtcgtcg gagaccagtg ggacacgctc tacgtcgaag ccgcggacgg
      541 tctggacgta ctcgctacgg tcgacgaagc cgtcctctgg gcaaacgagt tcatccgccg
      601 catcgccgca gcgcgcacgt agcgttttcc atgagcactg cgtccggcgc aggccttgtt
      661 gcacgtatgt tgcatgaaga aacccgccct gactatttga cctagtcaga acgggtttca
      721 actggggtga gtaacgggac tcgaacccgc gaccacctgg accacaacca ggagctctac
      781 caactgagct atacccacca tgtcaccaac gtgcccgtta gggcctgctc attctagcca
      841 catgcccagt cgaggtcgaa atccgcgagt gggtgggacg attcgcgtcc gatcgagggc
      901 aaaatcgctg aggtcggcga gtcgggctcg gacgcgttct gtcccgatcc cggcctgcgc
      961 cgggatgact tggagcgggt cgggacgctg ggtgcggcgg ggatgacttg gagcgtggtc
     1021 gggacgcggg gtgtggccgg ccgaccggtg tggcgggatg actgggatgt gggcgggctg
     1081 acgtcagctg tgggtggcgc ggatccggag ccaggctcag ctgccagtct tagtggcgcg
     1141 ggatccgtcg cagagctcag cttccggtcg cggtggccag atcggcggtg agcgaaccct
     1201 ggtgcttggc cgcgatctcg cgcaacgcgt cggtcggcat cccgggcgtg ggcacgaaaa
     1261 gggtctcgcg gtagtaccgc agctcctcga tcgattcgca gatgtcggcc agcgctcggt
     1321 gattgccgtt cttcgccggc gtgttgtagt aggtgcgcgg ataccagcgc cgagcgagct
     1381 ctttcaagct ggatacatcc acattccggt agtgcacgtg cccctccaga gtaggcatgt
     1441 cgcgggccaa gaacgctcgg tcggtaccga tcgtgttgcc cgccagcggc gcgcggcgcg
     1501 actcgggcac gtgctcgcgg atgtacgcca gcacccgctc ttccgcctcg gccatctcca
     1561 gcccatccgc cagcagcggc agcagcccgg agttcgcgtg catcgtgcgc acgaaatcgc
     1621 ccatctggtc cagcgcggtg tcgctcggct tgatcaccac agtgatgccc tcaccaagca
     1681 cattcagttc ggaatcggtg accaacgcgg ccacctcgat caacgcgtcg gccttcagat
     1741 cgaggccggt catctcgcag tcgatccaaa ccaagtgttc attcacccgt tcactctatt
     1801 gcctcaccgg tcggggcttc actcgcgata acgtccaatc catcaccgac gttggaggaa
     1861 cgaatgcccg ggttctccat ggccggctgc gaactgggcg tggccagcgc agcgacccag
     1921 atcgaaggcg gtcaactcga caccaactgg catcgctggg ccgcgtccgg aggcgccgtc
     1981 gacgggtcgt ccccggcccg cgccgccgac cactggaacc gggtctccga agacacctcg
     2041 ttgctcgccg aaatgggcat cggccactat cggctgggca tcgagtgggt tcgcgtcgaa
     2101 ccgagtcccg gggagttcga cccggcggcg atcgggcact accgcgagga attgtccggg
     2161 ttgcgggctg ccggcgtccg accgctggtc accctgcacc acttcaacct ccccggctgg
     2221 ctggcggacg ccggtggctg gctcgctccg gaagcgaccg aggccttcct tcgcttcgtt
     2281 cgccgcatgg tcaccgacct cggcgacatc gtggacgagt ggatcccgat caacgagccg
     2341 aacgtctacg ccgtcaacag ctacctgttc ggtctctggc cgcccgacga gcattcctat
     2401 cgccgcacca ttcgcgtgct ggagatcctc gctacggccc acatcgccgc ctacgaactg
     2461 atccaccggc ttcagcccga ggccaaggtg ggcaccgcca cgcaccttcg ggtgttcgcg
     2521 ccggccaatc cgctgaaccc gtggcaccaa ctggccgccc acatcaacga ccacctcttc
     2581 caacgggcac cgctgcgcgc catgagcacc ggacgcttcc ccgccccgct tcgccgtccg
     2641 tcctgggtac ggcccggtcg ctattacgac ttccaaggca tcaactacta cacgcgcagt
     2701 acggtcagcg ggctcgccaa cggagtggcg tcgaacgtgc cggtcaacga cctcggctgg
     2761 gaggtcttcc cttccggact gggcgaggtg gccgcctggg tgcacaccgc ctacccggcg
     2821 ccgatctacg tgaccgagaa cggcaccgcc gacgccgcgg acgccttccg ctcccgctac
     2881 ctctacgacc acttggcggt cgtcgcagcg tccggactgc cgatccggcg ctattaccac
     2941 tggtgcttca ccgacaactg ggaatgggcc gagggcgagt ccgcccgctt cgggctggtg
     3001 gcgctcgact tcgagtcgca gcagcgcatg atccgcgcgt ccggacgctt ctacgccgac
     3061 gtcatcgcca accgtggggt gaccacggcc gcctaccagc gctgggtcgc cggccaggag
     3121 tacccgacga acggctgagc cgggtctcga cggcggtcga tggtctggcc aggggctact
     3181 cggggtagcc ctgccagccg gaatcgtggc cgacgagccg ctcgcgcagc cccttcacat
     3241 aggcggcctg gccaatgtgc tgagcggtgt ccagcactac tgagaccaag cggacgccga
     3301 gcgtcaccgg cgggttccag cgcgtgtcga cgacccggtc gaggtcgtcc ctggccaatg
     3361 cgtccaggac ctgttcggtc tggaccgcga ccgctttcgc gtagccgagc agcaggtcgg
     3421 ctcccggcac gtcgaactgg ccaacctctt caggtgactg cccgaagccg cccgcatcgg
     3481 ccgcgtaggg caaatcgaac cgctcctgcc agccctggaa ccagacctgg tcgaagccgg
     3541 ccagcggcgc gaggtggtca tcctccactc gcagcaggtg ccagaccagc cagccgatcg
     3601 ggttggcgga cgggtccgga cgccagcgca ggtcgtcccg gctcagacca ttcaggacgg
     3661 cagggacgag ttcgtggacg cgtccgaacg catcgcgtac gacactgatt gactccatgc
     3721 cgcccatcct gccaagtgca gccgacaacc ctcggcggga taccatcgtg acaaccggcc
     3781 cccgtagctc agtggataga gcagcggcct tctaatccga cggtcacagg ttcgagtcct
     3841 gtcgggggcg cccactcagt ccgacccctg cccacctgcc tggaagcccc gatgaccgca
     3901 tccgttgacg accggcgcac gctggctgcc cgcaacgcca ccatgctggt ctttgcagtc
     3961 aacggcttcg cgttcgcgtc ctggatgtcg cgggtgccgg atgtgcgtga agcgctggcc
     4021 ctcaccccag gtgagctcgg actcctgctg ctttccatct cggccggcgc gttgtgcggc
     4081 ctgccgctgg cgggccgggt cacccaccgc atcggtgccc aggcgaccgt ccgggtcggg
     4141 atggggctga tggtgtccgg cctggtgctg gccgcgatcg cggccgaagc gcatgccggc
     4201 atgtactggg tggctccggg gctgttcctg gtgggccttg gcagcggcgt ctgggacgtg
     4261 gcgcagaatg tcgagggcac catcgtcgag cgctcgctgg gccgggcgat catgccctgg
     4321 ttccatgcgg gcttcagcgg cgggacggtg ctcggcgcgt tggtcggcgc cggcatgacc
     4381 gcgctgcacg tgccggtgct ggtccacctg gtcacggtcg cggtgctctc cgcgctcgcg
     4441 ctctggtggg gcacactcag cttcctgccg gccttcggcg agtccgagga cgccccaact
     4501 tcgcctgccc ggggaaagtc ggcctggctg gagccgcgca ccctgctgat cggcctaatg
     4561 gtgctcgctg ccgccttcgc cgagggcact gcgaacgact ggatggccgt ggccttcgtg
     4621 gacggtcaca atctggacaa cgcgatgggc gtggttgcgc tcgcggtgtt cctcaccttc
     4681 atgaccgccg gacggatctt cggcaccggc ctgctggacc gctatggccg cgtcccggta
     4741 ctgcgggggc tgttcgccgc gtccatcatc gcgtgtgcgc tggtcgtctt cggcgatact
     4801 tggctggcgt tcatcggcgt cgccatctgg ggtgtcgggg ccagcctggg cttcccggtc
     4861 ggcatgtcgg cagcagcgga cgatcccgct cgggctgccg tccggatcag cgtggtggcc
     4921 acgatcggct acaccgcctt cctcgccggg ccgccgctgc tgggcttcct cggcgaccac
     4981 ttcggcatcc tgcgtgcgct gctggtcgtg ggggcggtgt cgatcctggc catgctcgtg
     5041 gtgccggcgg ccaagccgct gccgccgaac ccggagagct agccggcagt cgcaagtaga
     5101 gtcgacgaca tcacttcggt ggaggagtcg ccagatgatc gagagccgtg aagtcgagtt
     5161 gctggccggc gtgcctgagg gcctgttcat cggcggacgg tggcggtcag cgtccgacgg
     5221 cggcaccttc gaggtggcgg acccggccac cggatcaccc gtgaagcgcg tagccagcgc
     5281 gacggttgcc gacgggttgg cggctctgga cgcggcgtgc gacgcgtttc cggcgtgggc
     5341 gaagacgcca tcacgggagc gcgcggagct gttgcgcagg gccttcgacc tgttgcagga
     5401 gcgcaaggag gagttcgcgc tgctgatgac gatcgagatg ggcaagccac ttgccgaggc
     5461 ccgcggtgag gtggcctatg gcggcgaatt cctgcgctgg ttcagcgagg aggcagcccg
     5521 aatccagggg cgctacgggc ccaatcccga aggcaccgga cggatgatcg tctctcagca
     5581 tccggtcggg ccgtgctacc tgatcacccc gtggaacttc cccttggcga tggccacccg
     5641 caaggtggcg ccggctttgg ctgccggctg cactgtggtg gtgaagccgg ccgcgctgac
     5701 gcccctgacc acgctctact tcgcgaaact gctcgctgat gcgggattgc cggacggtgt
     5761 ggtgaacgtg gtgaccacga aggcgtccgg accagtctcg gcggcggtcc ttgccgatcc
     5821 gcggctgcgc aagctgtcct tcaccggttc gaccccggta ggtaaggaat tgctcaagca
     5881 ggccgctgat ggcgtcctgc gtacatcgat ggagctgggt ggtaacgcgc ctttcctggt
     5941 cttcgaggac gcagacctgg acaaggctgt ggacggagcg atcctggcga agtttcgcaa
     6001 catcgggcag gcctgcacgg cggccaatcg gttcattgtg caccggagca tcgcggcgga
     6061 gttcgccgct cgagtgactg cgcgcgtgcg cgagttccgc accggacgcg gcaccgagga
     6121 aggcatcacg atcggcccgc tgatcgatgc ggacgccgtg gccaaggtgg acggactggt
     6181 ccgcgatgcc gtggcccgcg gagcttcggt gcagaccggc ggagcgccga tcgacggtcc
     6241 gggaaccttc tatgcagcga cggtgctcac cgaggtgccg cccgacagct cgatcgtgcg
     6301 ggaagagatc ttcggtccag tgcttgccat cgtcactttc gcggacgagg acgaagccgt
     6361 ccggctggcc aatgacaccg gctacgggct gatctcctac gtcttcaccg agagcaacgc
     6421 gtgcgggcaa cggatgatcg agcgcctgga gaccgggatg atggcgctca acgtcggtgt
     6481 ggtctcgaac gccgcggctc cgttcggcgg ctggaagctt tccggcatcg gacgggaggg
     6541 cggcgcggag ggcatccacg agtacctgga gaccaagtac acgctcacgc ccaacccctt
     6601 cggttagggc gaaacgaagc cacgcagagt caggtcgctg gaacccctgc gcaacctttg
     6661 acaagccgca cgggcggcct gcgtccacga cgtaggctgc cacaacctgg aatgcgcgcc
     6721 gcgaggagaa cccggatgag tctgcaggag tttaaggcca agaccatcac gggccaggac
     6781 caggccctgg ccgcgtacct gggtcaggtc atcctggtcg tgaatacggc atccaagtgt
     6841 gggttcaccc cgcagttcgc cgggctggag aagctctacg ccgacctgcg cgcggacggc
     6901 ctggtcgtgc tcggcttccc ttgcgaccag ttcgccaatc aggaactggg cagtgatgcc
     6961 gagaccgcag agttctgtca gctcaactac ggggtgacct ttccgatgtt cgcgaaggtc
     7021 gaggtcaacg gtaggaacgc gcacccgttg ttcgcctggc tgcgcagcga gcagggcggg
     7081 gtgctgggcg atgcgatcaa gtggaacttc accaagttcc tgatcgaccg tgagggccgt
     7141 gtggtgcgac ggtacccgcc cacggtagac cccgccgcca ttgaggccga cgtccgggcg
     7201 ctgctgtagc ccgagagccg ggaccaggtt taccccgact gatcccggca gcgcgggtcc
     7261 agcgtcgtga gctcgggcaa agtcggggga cggttccttt ctgcgtcgca acttgccgct
     7321 ggctagggca ttctgcagtt cggggacggt tcctttccgc atcaaaggtc ggccgcacgt
     7381 tcggacgcga accgcactct gccccgcaaa gcaactgcct gatctccggc accgacctgc
     7441 caactgcgac cgcgtgctca gtgatgtact ggaacccgcg agaagcttca actaatccga
     7501 tgccgatcgg ctctttaccc ggcacgatag gcgcatggaa tccgagaagc ccaagaaact
     7561 gcccaaggac gtgtacgagg ctgaactgct acggctgcag gcggaactcg tgaacatgca
     7621 ggaatgggta cgcaccaccg gagccagggt ggtggtcatc ttcgaaggtc gcgacgccgc
     7681 cggcaaaggc ggtgcgatca aacgggtcac cgagtacctc aaccctcgga tcgcccaggt
     7741 ggtggcccta cccgcgccca ccgaacggca gcgcaccgag tggtacttcc agcgctacgt
     7801 gcagcacctg ccctcggccg gccagatcaa gctcttcgat cgctcctggt acaaccgggc
     7861 cggtgtcgag cgggtgatgg gctattgcac gccggaggag catcggcgct tcctgcggca
     7921 atgcccgatc ttcgagcgga tgctgatcga ggacggcatc atcctgcgca agtactggtt
     7981 ctcggtctct gaccgggagc aggagaaacg attcaagtcc cggctgaccg atccgatgcg
     8041 gcagtggaag ctgtcgccga ccgacctgga atcgctgacc cgctgggagg actactcccg
     8101 ggccaaggac gagatgttcg tgcacaccga cattccggag gcgcgctgga acgtggtcga
     8161 atcggaggac aagaagcggg cgcggctcaa catgatcgcc gacctgctca acgccatccc
     8221 gtacgaacgc gtgcacaacg accagctcaa gctgcccaag cgtccgccgt cgacgggcta
     8281 ccggcggacg gatcgcggcc tgcagctcga ggtgcccgac tgggccgcca ccctgatcga
     8341 caaggaaccc ccaccgaagt acgtcgaccc ggaggacgag gactgaccgg tgcgcggcga
     8401 cgggatcgcc ggttcaggct gaatcgaact ttcacgccgg cgatccggac cgacgccggg
     8461 gaatattccc tggcgtcggt cagtttcgcc ggcgcgaaca acagcggtac gacccgaggt
     8521 ctggtcaggg ccagtgcata gctcagccca accggctgaa accggtcacg tgcacgatca
     8581 gctcaccggc ctcgtcgcac gcgtccaggt cgatggtggc ggtgatcgac cagtcgtggt
     8641 tgccttcggg gtcggcgatg agctggcgta cctgccaggt gcgtccggcg tcctcgatga
     8701 tcagcatcgc cgggccacgg gcgtccgcgt cggtgatgat ctcgtcgtgc tcgtcgtagt
     8761 agtcgccgag tgcgtcgtcc cagtcgcgga agccgccggc ccggtcggga tcggctgagt
     8821 cgagctcgag caggccgtcc acatcgtcgc gagcggcgag ctccacccgc cggaacatcg
     8881 cattgcgcac cagcacgcgg aacgcccgca ggttgccggt cacttggcgc tgcggtgggg
     8941 gagcgacctc cagcgggcgc gcgccggaca gcgtggtgag ctgttcccac tcgtccagta
     9001 gcgaggagtc agtgagccgg gtgacctcgc cgagccactc gatcaggtcc tcgagttcct
     9061 cggtgcgcgc cgcctccggg acggtctggc gcagcgcccg gaaggcgtcc gacagatagc
     9121 gcaacaacaa cccctcgctg cgctgcaccc ggtagaacgc gacgtattcg gtgaacgtca
     9181 tggcgcgctc gaacatgtcc cgcaccaccg acttcggcga caccggggtc tcggccagcc
     9241 acggatggtg gccgaggaag acctcgtagg cgtgctccag cagttcagcc agcggacggg
     9301 gccaagtgac ttcttcgagc agttcctgcc gctcggcgaa ctccacgccg tcggccttca
     9361 gctctgcgac cgcctcgccg cgcgccttga actgctgctg cacgagcacc gcgcgtgggt
     9421 cttcgagcgt tgcctcgatc acgctgaccg cgtccagcgc gtagctgggt gattccaggt
     9481 cgagcagttc cagcgccgcc agcgcgaagg cggacagcgg ctggttgagc gcgaaatcgt
     9541 gctgcaggtc gacagcgagt tggtgccgtc ggccaccggg cgtcggctcg ggcaaccgaa
     9601 ccaccacccc tgcggccagc agcgagcgcc cgatggcgac cgcgcgccgg acgagggcgc
     9661 gccgcgtccc cggatcactg gtggtggcgc agatcagtga ccacaccgcc accccggtgt
     9721 cctggtcgcg ctgcagcagg ttcagcagta gggcgtggct gattcgcagc cgggcgacca
     9781 gcggctcggg aggggcggcg atcagcttgg tgaaggtgtc ctcggtgaag ttcacgaagc
     9841 cctcgggtgc cttcttgcgc tgcaccttgc gttgcttctt cggatcgttc ccggctttcg
     9901 ccagcgcaac tgcgttttcg atgacatgat ccggcgcctg cgccgcgacg tagccaacgg
     9961 tgtcgaagcc ggggcgcccg gcgcgtccgg cgatctgatg gaactcgcgg gcgcgcagca
    10021 gccgctgccg ccggccgtcg aacttcgaaa gggacgcgaa gagcaccgtc cggatcggta
    10081 cgttgatccc gaccccgagg gtgtcggtgc cgcagatgat gctcagcagg ccacgctggg
    10141 ccagcgtctc cacaagtcgc cggtagcgcg gcagcatgcc ggcgtgatgg acggcgatgc
    10201 ccgagttcag caacttggcc agcgtcttgc cgaagcccga gccgaagcgg aaaccctgca
    10261 tggcctcgac cagttcgcgg cgatgtgagc ggtcgatgat gcccgaactg agcagggcct
    10321 gggcgcgctc gacagctgca gcctgggtgg catgcacgat gtagaccggc gcctgggcgt
    10381 cccgcaacag cagttcgacc gtctcctgca gcgggcgtaa cgaccacgaa taggtgagcg
    10441 gcaccggacg gacggcgtcc gagatcacgc tggtgccgcg tccggttcgt gcagagaggt
    10501 cgcgagcgag ttggctgacg tcgcccagtg tggccgacat gatcacgaac tgagcctgag
    10561 ggagttcgac cagcccgacc tgccacgccc acccgcggtc acggtcggcg atgaagtgga
    10621 actcgtcgat caccacctgc gctacgtcgg cggcccggcc ctcacgcagg gcgatgttcg
    10681 cgaggatctc ggccgtgcag cagatgaccg gggcttcggc gttcaccgcc gcgtccccgg
    10741 tgaccatgcc gacccgctcg gcgccgaaga tgtcgcagag cgcgaagaac ttctcgctga
    10801 ccagagcctt gatcggtgcg gtgtagtagc tgcgcccgcc gccggccaac gcggcgaaat
    10861 gcgcggcggt ggcgatcagt gtcttgcctg aaccggttgg tgtggtgatg atcgtgttcg
    10921 cgccggtgag gatgtcgacg atcgcctcgt cctgatgcgg gtacaacgtg atgccgtccg
    10981 ccgccgcagc gtcgatgaag gccgagtaca gggcgtcggg gtcggtgggc cccgcttcgg
    11041 ggatcaggtc ggttagtcgc atgacgctcc atcgtcccat gcgaggacgg gcgcgtcggc
    11101 cgacggcgct cgccggctga aactcagcaa ttcttggccc tccggacgcg ccggacaccg
    11161 ctgtcgaaag gggtgcggag gtctagattc agcaacggta atcagtcgcc gtttccgatc
    11221 gtgatgtcgg gggnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnaaacggt
    11341 ggcactgact tggtggggat tccgaccttg cagccgacca tgcggtgacc tgggattacg
    11401 ggggcagtcc ccggtcaccg tggcatttgg cccgaatccg gcacagcccg cggcgttggg
    11461 cgggtgacgt caggcgcgcg gtatcaactt caggtacgag tgcagtagcc gacccagttc
    11521 gcgcagccgc tccggacgct catcctccgg gtagttgaag gtgtcctgga tcaccgcgtc
    11581 cgcggcgacg aacgccacct gggccatgat gctggtggtg tcgcggtcct cggggcgggt
    11641 gccggcagcc tgcatcagtt cggccacctt cgacgccatc cgagcccggt gtgcctgacg
    11701 ctgtgcctgc agttcagggg aactgccgaa cgaacgcagc ggacgcgcag cctcctcctc
    11761 gcggtagatc tccaccaccg attcgagtgc gtactcgacc ggatccccgg acggcggctc
    11821 ccgccgggca gaagccacgg ccccttccag caacgcctcc aggcgggcgt ggtagcgcca
    11881 taccaacgcg ccgacgatgg attcacgatc ggggaggtac tgatagagcg cgccgacact
    11941 cactccggcg cgttcggcga cccgaggcag gttgatcgcc tccggaccct cctcgcggac
    12001 cagggcgtcc gctgcaacca gggctcggga aaccttgtcc cgggagcgct tctgagtggg
    12061 tacgcgacgc agcggcagcc gctcccaggg gagttcattc gccatgccca ccacctctag
    12121 ccaaacctga ttgtggtttc tttcattgac cggtgccggc gccttggaag cgccatcatc
    12181 gtcggtcctg cccgaatccg cagccgctgc cgcgggtcga cctgccgcac caggtcgctt
    12241 catcggttca atctgaactt tagtgcccgg gcggccggcc tgggcgccgt ggcggcggtc
    12301 ggcgatccca ccctgaatgt ggtaaccatc gtagatctcg gcggacgaat cgttcatggc
    12361 gacctcctac ccacttcggg gctcggcgtt gcatccgggc cccccttcca gcgannnnnn
    12421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnagcgag gagtggacgg ggagtttgca
    12541 gccggctggt ttggccggtg cggaccgtcc agtgcctact caaaggtggt ttgccggttg
    12601 ttgacgcggt gacaggtctc ggcaaggtaa cgatttgcgc tcgtcgtgag aaatcaggtt
    12661 tcaagacgat gagagcgctt tctttctgtg ctagcttgcg agttgttggc ttcgaagagg
    12721 cggtcagcgc cagctcggca acgactggta ccgccccacc aggcacatgt cgaccggtcg
    12781 aggaggatca gtggagcagg ttcggatgaa tctggccgat gctgagtgga cggtgcgtgc
    12841 cgagccttcg tcagatccct tcgatgaggt tcccagcgat ctcagggcgg cgctcagcga
    12901 tggaatcccg gccaccgtgc caggttgcgt ccacaccgat ctgatggctg ccggactgct
    12961 ccgcgacccg tacctgggca ccgctgaggg cgagcagcac tggatcggcc gacagacctg
    13021 gacctacacc ggacgcttcg acctcgaaga ggacgacccg ctgctcgaag ccgactactg
    13081 cgacctggtc tgcgatggcc tcgacacggt cgccgacgtg tactgcaacg ggcacaacct
    13141 cggccatagc gagaacatgc atcgccgcaa cctgttcgac gccaagtcgg cgctggtggc
    13201 aggtgcgaac gtgatcgaga tcgtcttcgc gccggccctt gagcacgccg aacgcatggc
    13261 cgccctggtg ggggagcgca tccacgtcga acgcgacccg tacaacctga tccgcaagat
    13321 ggcctgcaac ttcggctggg actggggccc acggctgatc accgccggca tctggaagga
    13381 catcgagctg cgcggctggg acaccgcgaa ggtgctcgac gtcagcgtcc aggccacggc
    13441 cagccaggcc gacgacggca cctggatcgg ccaccttgag gtcaacgcgc agctcgtcgg
    13501 caagatccgc ttcgccgcgc tcaccgtgaa gctcaccggc aacgaccttg aagccacggt
    13561 cggtgtgaac gccgaggact ggacgcgggc caccctcgac atcaccggca tccagccctg
    13621 gtggccgcac agcctgggcg accaaccgct ctacgacctc gagatcctgc tgcacaacga
    13681 cgtggcggtc ctcgatcgcg tgcatcgtcg caccggcttt cgcggcaccg cgctggacac
    13741 caccgcggac gcggacggaa ccggcgccaa gttcgccctg gtgatcaacg gcactgaggt
    13801 cttcgtccgc ggtgccaact ggattccgga cgactgcttc ccgacccggg tcaccccgca
    13861 gcgctaccgc gagcgcattc agcaggccaa ggacgccaac atcgacctgc tgcggatctg
    13921 gggcggcggc atctacgaga ccaaggcctt ctacgacgcc tgcgacgaac tcggtgtgat
    13981 ggtgtggcag gacttcaccc tcgcctgcgc ggcgtacccg gaggaagagc ccttcgccag
    14041 cgagttcgag gccgaggcgc gcgacaacat catgcggctc tccgcgcacc cgtccctggt
    14101 gatctggaac ggcaacaacg agaacatctg gggcagggac gagtgggact gggccaacca
    14161 gcccggtggc gacctcacct ggggtgcggg ctactactac gagatgttcc cgcgcatcct
    14221 ggccgagctc gaccccagcc gcccgtactg gcacggcagc ccctcgtccg gttcgccgga
    14281 gatccggccg aacgacccga acttcggctg catccacatc tgggacgtct ggaacgagcg
    14341 cgactacacc ggctacgacg actactcgcc gcgcttcgtc tccgagttcg gctatcaggg
    14401 gccggccacc tacgccacct gggcgcgcac ccttgatgtg gccgaccgtg gcgctacgac
    14461 cagcgcgatg ctggtccacc agaaggccgc cggtggcaac ggcaaactca acgcaggaat
    14521 tgccgggcac ctgccgcagc cgggcgaaag cgtcgctgac ttcgacgaat ggctctacct
    14581 gatgcagctg cagcaggcca acgcggtgcg ctacggcatc gaccgttggc gctcgctgcg
    14641 cggccgctgt cacggctcga tcgtgtggca actcaacgac tgctggccgg tcacctcctg
    14701 ggcagccctg gatctcggca ccaatgccgc cggcgttccg gtggcccgcc gcaagccgct
    14761 gtggtacgcg ctgcgttcgg cgtacgccga ccggctgctc accttccagt tcgaggaggg
    14821 ccagtggcgg cttgccgtcg tcaacgacgg cacggacgcg tggctggtcg atggcaccgt
    14881 ccagctccgt cggctgaccg gtgagatcgt ctgggagaag cggttcagcc gcgaggtgcc
    14941 ggcacgggcg cgtgtcgacg tgaccgtcac cgaccccgtt ccgctcgaat ccggcctcgc
    15001 gctggtcgcc accgcggacg gcgccgagcg ctgcgtccgc ctgctgctgc cggacgtcaa
    15061 tgccgacctg ccgacgccgg agttcgaggc ccgggccgtg gccaccgcgg acggagccgc
    15121 gatcaccgtg accgcgaagt ccttcgtccg cgcactctgt ctgttccccg accgggttga
    15181 tgaggacgcc tgggccgaca gcgcgctgat cgacctcttc ccaggtgaat cgcaccagtt
    15241 caacgtccgc gggaccttca ccgcgagcga gctggcccgg ctcaccgacg cccccgtcct
    15301 gcgagcactc gatccccacc cgaccccgag cgaggcccca tgagggtttc ctgaccgtcc
    15361 cggccgtaag gccggcagcc agcactaacg agcaagtccc attccgaaca tccacccgaa
    15421 accggcccaa tggagggcca gataactgga ggaacaaatg tcagaatcca tcttcgagaa
    15481 gccgtcgcgg cggtcgttcc tgatcgccgg tggcgtcgtt gccggtggga tcgccctcgc
    15541 ggcgtgctcg tccggcggcg cggccagccc gaccagcggt ggtgccacca gcggcagcgc
    15601 ggctccggca gagaaactcg gcatcggcaa caacggtaag ttcggcgctg gtcgcaaggg
    15661 caacaccgct gatgccctgc tgatcgccgg cttccagtgg ggccccccgg ccaccttcaa
    15721 cccgctgtcg ccgaccgctg cctggccggc agccgccaac gtcatgcaga tcgtctacga
    15781 gagcctgctg cgctggaaca tcgcgaccgg cgagatcatg ccgggcctgg cgaaggagta
    15841 caaggtcgac ggcaccacca gcatcaccct gaccatgcag gacggcgtca ccttctccga
    15901 cggctcgccc tgcaccgcca aggacgtcgt catgtcctac gagctgggca agaagaacac
    15961 cggtatcacc gtcgcctcct tctggcagct ggccgactcg attgaggcca ccgacgacaa
    16021 gaccgtcgtg gtgaaggtca accagaagac caagaacgtg ggcaacgtgc tgcggatgct
    16081 caccgagacc tacatcctgc cgtcggcggt cttctccggc atcgcggatg acaagctgcc
    16141 caccgaggtc atggacaagc cgatcggtac cggcccgttc accctcgaga aggccgacca
    16201 gacccaggtc gtgctgaccc ggaacgacaa gtactggggc cagaccttct acggcggcct
    16261 gccggtgatg aagcagatca tccacccgat cttcaagtcc aatgaggacg gcaacatcaa
    16321 gttccaggcc ggcgagctgg acgtcatgca gcagttcgtg tcccagatct ggaagatgtg
    16381 ggagggcggc aagccggtcg gcacctacct gaaggagaag ccgtactacg tgccgggttc
    16441 gatgccgatg ctgctgatga acaccaccaa gaagggcctg gacaacccgg ccgtccgcaa
    16501 ggcgatcgct tcggccatcg actacgcgtc catcgctgag acggccatgt cgggctactc
    16561 cgcagaggtg aaggcttcgc tgatcctgcc cgagggcgcg gagagcaagt tcttcaacga
    16621 agcggacgcg accgccaacg gctggaagta cgacccggcc gctgcggaga agatcctcac
    16681 cgacgccggc gccaagaagg gctcggacgg catctacgag atcgatggca ccaagctcgg
    16741 cccgtacaag ctgatcaccc cgaccggctg gaccgactgg aacgcgtcgt gtgagatcat
    16801 cgcgaagagc ctgaaggcca tcggcatcga ctgctcgacc aacttcccgc agcaggccga
    16861 gaccacccag gccatccagg gcggcacctt cgacatggcg gtctggtacg tctccggcgt
    16921 gaacccggcc accccgtggg cgcggttcaa ggacatcatg agccaggccg aaatgcagcc
    16981 gctgggcaag accaccttcg ccaactacgg tcgctggaag aacgacgagg tcgaggcttt
    17041 gttgaccgag gcttctcagg ctgtggacga cgccgcgaag aaggccgcct acgacaagct
    17101 ggacgcgctc taccgcaagg aggtgccagc ctgcccgatc atgtaccgtc cggacgagtt
    17161 ctacgagtac aacgcgacca acttcttcaa ctggccggat gagaagaact cctacgcgcc
    17221 gccgatgttc cgtggcgcag gcaacacctg gatgtacaag atccagaaga tcgccggctg
    17281 agtcagcgac gatctagaga agttggactg ccatgggtct acggcgctat ctcgccaata
    17341 agaccgtgtg gtatctgggt gcactcttgg tagcagtggt tttgaacttc ctgctaccaa
    17401 gggtgatccc gggtaacccg gtcgacgtga tcgtctccag aatgggccag ggcggccaat
    17461 cctcggaggc acaggcacgc gtgtacgaac agttcatgcg tgagttcgga cttgaccagc
    17521 cgttatggag tcagttcgtg acctatgtga ccagcgcctt ccaaggcaac ctgggcgcgt
    17581 cgttctcgtt ctatccgaca ccggtggccg agctgatcgc ccagtcgctg ccttacacct
    17641 tggtactcca acttccagcc atcattctgg gttggatcat cggcaacgtc ctcggggccg
    17701 tcgccgccta caagggcggc tcctgggaca agtcggtgtt caccacctcc ttgctgttct
    17761 cctcgatgcc ctactactgc ctttcgatca tcctgctcta cacgctggcc gtggtgctgg
    17821 ggtggttccc gccgggcggc gcctactcac tgggcatgag tccggagtgg agctgggagt
    17881 tcttctccga cgcgctgcag ttctggtggc tgccgttcat ctcgctgctg gtgatcaaca
    17941 ttggtggcca ggcggtcggc atgcgttcga tggccatcta cgaactcggc tccgactacg
    18001 tcaactactc ccgcagcctg ggcatcggcg atcgcaagat gaccacctac atcttcaaca
    18061 acgcgatgtt gccgcagatc accggtctcg ccctgagcat cggcacgatg gtcggcggcg
    18121 ctctgatcac cgaactggtg ttcagctacc cgggcatggg ctcactgctg ttcaacgcga
    18181 tcgcctccaa cgactacccg gtcgtgcagg cggtcacgct gatcatcacg gtggccgtgc
    18241 tcgtggccaa ctacgcggtc gagatcctct acggcttcat cgacccccgc atccgcgctt
    18301 cgaagtccgg ggagaagtga catgaccaac atccagagca gcgcggtcgc tgccgagacc
    18361 gaaggtcccc tcctcccgcc ggagccgtcc acaccaaagg ccaaggccaa gccagcctgg
    18421 cgcagcctga acttcgggcc caggctgtgg gtctcggccg ggctgatcgt gatcatcgcc
    18481 ctgctggcct tcgtcggccc gctcttctac ccgttcgcac cggacgagaa ggtcggctcg
    18541 ctctacgacg gaccatcggc cgagcacatt ctcggcacgg aaaacttcgg ctacgacgtg
    18601 ctcgccgtgc tcatggccag cacccgcaac tccctgatca acggcttgct cgcgggcacg
    18661 ctggctgtgg tgatcggcgt gttcatcggc atcctggccg gctacatcgg tggctggctg
    18721 gaggagctgc tgatgggcat gaccaacatc gtgctggcga tcccgtccat cgtgatcctg
    18781 atcctgatct cgatctcgct gcagagcact gacattccgt tcctgaaggg catcgtcggg
    18841 ttggccttgg tgatcggcat cacgtcctgg ccctggacgg cccgagccgt cagagcccag
    18901 gcgagttcgg ttgccactcg agaacacatc gacgtggcca agctgtcggg cgcccgcact
    18961 ggcggcatcc tgatcttcga tgtgctgccc tacctggcca gctacgtggt gatggcctgg
    19021 gtgctccagt tggccagcgc catcctggcc gagtcgacgc tgtcgatgct cggtctcggt
    19081 ccgtccggca ccaactcgct cggcatgcag ctgcactggg cgctggcgtt ccaggccgtg
    19141 gccaccggcg cgtggtgggc gttcctgccg cccacgatcg tgctgaccct gatcagcttc
    19201 gcgctgctct acctgcagtc gtcgctggac gaggtgttca acccgaggct gcgccgcggc
    19261 aagaagaagc acctggccca ggccgcagcg cgtcgcgccg aagcagccaa gcagctcgcc
    19321 gagcagaacc agcgcgcgga ggtgaccgca tgagcggcat cttgatgcgg gccagcaacg
    19381 tgaccgccgt ctacgcagcc gacaccggca aggacgtccc ggccgtcgac aacgtcaccc
    19441 tcgaggtgcc ggacgggcag atcgtcggcc tcgccggcga atccggctgt ggcaagtcca
    19501 ccctcggcaa tgccttggcc atgatcgcga atccgccgct gtacgtggtg gccggcaagc
    19561 tcgagatcag cgacaccgag atcgacctca gcacgctggc caagggtgcc aacgaggtgc
    19621 atcgcggcca ccgcggggcg acggtttcgt tgctgccaca aggcgcgatg aacgcgatca
    19681 gcccgaccct gcgaatccgc gacctggtcg tggacgtgat gcgggcgcac aagcgcgaca
    19741 ccaccaaggc cgaggctctg gaccgggcca ccgaccggct gaagatgctg gagatgccca
    19801 cccgggtgct cgactcctac tctcaccagc tctccggcgg catgaagcag cgcatggtca
    19861 ccgtgatctc caccctgctc aacccgcagt tgctgatcgc ggacgaacca acctccgctc
    19921 tcgacgtgtc cagccagaag gcgctgatcg agatgctgct ggcgatggtc gaggcgaaga
    19981 tcatgcgcgg cgtgatcttc atcacccacg acctgccggt gctcagcatg gtcaccgacc
    20041 ggctggcgat catgtacgcg ggcaagatcg tcgaggacgg tcccaccgag gaactggtca
    20101 acaacgcgcg gcacccgtac acgtccgcgc tgctgtcgtc ggttctcgac ccaaccgagg
    20161 aaacccgcac caagcacgtg ctgggcatcc ccggttcgcc gccgaacctc ggcaacccgc
    20221 cgtcgggctg ccgcttccac ccgcgctgca agttcgcgat gccggcctgc aagctggaag
    20281 agccggcgtt cctgcacgag ggtgagcatg aggtggcctg ctggtgggcc atggagcact
    20341 tgaacgagac cgtcccgatg gaggtgaacc aggcatgagc gatccgaagt cgctggaccc
    20401 gggggccatt ccgcccgcag gctcgcccgt cctggcagcc agtcacgtga ccaaacagtt
    20461 ccgggtctcg ggtggccaca tcactgcggt cgacgacgtc agcgtcgagt tccacgccgg
    20521 caaagtgctg gccgtggtgg gggagtccgg ctcgggcaag tcgaccctcg cccggatgct
    20581 gcttcggctg atgccggtaa ccagcggcac catcacatac ctcggcaagg atgtgaccaa
    20641 catccggggc gctgaactgc gggcctactg gcgcgacgtg caggcggtct tccaggaccc
    20701 gttcgcatcg ttcaaccagt tcttcaccgt cggtcacatg ctgcggcggg ggatgggtgc
    20761 cgccgggttg tcggcagaga cgctcgacga gttgatcgag acctgcctcg gctacgtcgg
    20821 cctcaagccg gcggacgcgg tgcacaagta tccgcaccag ctctccggcg gtcagcgcca
    20881 gcgggtgatg atcgcccggg cactgatgat gaacccgaag gtgctactgg ctgacgaggc
    20941 cacctcgatg ctggacgcgt ccctgcgcgt gagcgcgctg aacgtgctgc agcacctgcg
    21001 cgaaaacctc ggcctgaccg tgctgttcat cacccacgac atcggtcagg cctgctacct
    21061 ggccgacgaa gtcgtggtaa tggagcacgg cctggtcgtc gagcagggca ccaccgacgc
    21121 ggtgatcttc gacccgaaga acgcctacac caagcgcctg ctggccgacg tcccagacct
    21181 gaagggcagc cttaagagcc gcaagctcga ctgagtaacg cattccccgg ctggacgggc
    21241 tcagacccgt ccagccgcag aaaggaccac ccatggacat cgaattcccc agttccttcc
    21301 gcttcggctc ggccacttcg gcgtaccaga tcgaaggagc ggctaccgcg gacggtaagg
    21361 aggagtcgat ctgggacgtg ttctgccggg tgccgggcgc gatcttcaac ggcgaggacg
    21421 ggctggtggc ggtggaccac taccaccgct accgcgagga catcgcgctg atgcgcgaac
    21481 tcaacctcgg cacttaccgc ttctcggtgt cctggccgcg ggtgctggac gccgagggcc
    21541 gcgtcaacgc caagggcatg gacttctact cccgagtggt cgatgaactg cttgccaacg
    21601 acatcacacc ctggctcacc ctctaccact gggatctgcc ggcctcgctg cccggtggct
    21661 ggaccaaccg gaacaccgcc aagcgcttcg tcgactacgc ccaggcggtg cacgcgcacc
    21721 tggctgaccg ggtacggatc tggacgaccc tgaacgagcc gtggtgctcg tcgttcctca
    21781 gctacgccgg tgggcaccac gccccaggcc agacggagcc ggtggccgcc gtcagggccg
    21841 cgcaccacct gctgctggcg cacggctgga cgatccaggc cctgcgggct gccgacccgg
    21901 acgctgaact gggcatcacc ttgaacttca ctccggccat cgccgcgtcc gatgaatcag
    21961 cggacgtgga catcgcgcgg cggctgaacg gcaccaacaa ccgctacttc accgatccga
    22021 tcttcaccgg cgagtacccg gccgacgtgc tcacggacct ggcttcgatc tggccggacg
    22081 acctggtgca cgacggcgat ctggcagcta tcgccacccc gatcgacgtg ctcggcgtga
    22141 actactacac gaccaacctg gtggctgccc ccgcggccgg tgccgagccg ccggccggcg
    22201 ccagcccgaa catcactgcg ccggaggcgg tcgtggtcga tcgcgggctg ccgaagaccg
    22261 cgatgggctg ggacatcgat ccgagcggcc tgcgcgacct gctgctctgg ctgcacggca
    22321 cctacactgc ggccgccggc gtgtcgctgg tgatcaccga gaacggggcg gcgttcgacg
    22381 acgtcgcgga cgcggacggg tttgtcgacg acaccgccga ccgcctcgcc tacttgaagg
    22441 gccatctgac tgccgtgcac gaggcgatgg aagccggcgc cgatgtgcgc ggctacctcg
    22501 cctggtcgct gctggacaac ttcgaatggg ctttcggcta cgccaagcgc ttcggcatcg
    22561 tgcgcgtgga cgaggaccag cgccggactc cgaaggccag cgcccgctgg ttcgcggaag
    22621 tggcccgcct cggcggcttc gacacctgag gaaccgctgc tcaacgccgg tcaacgctgg
    22681 ctgagcctga cgaagcccgt agtcaccgtg cggatggacg acccaacgcc cggctcaggg
    22741 atcgtcaatc agcgtttacc caaggctgag cgcccgttcg cgggcagggt gcccgagtag
    22801 ggtggcggcc atgcgcgcac caatcgctct gcagccgacc agccaccggt caactcccgg
    22861 ccggtctcgc tcgaatctcg gctggtcgag cttgtcgaga ccatcgttcg cgctgctgct
    22921 cgcactgggc ctgagcagct gtgcggtggt gccgccggcc ggcatcagtc cgtccggccc
    22981 cgccagccag ccggtgccca gcgtctcgcc gagcacgccg ctgggcacgc ccacgccctc
    23041 cgccaccgac gtgtccccgt ctgcgacacc gagcgtgtcc ggctcgccca caaccacgcc
    23101 cagcgcacca gcgacggcac tgccggccaa ggggaagggc aagctggtgc tgtaccagga
    23161 gaacctggtc tccgaccaga tgacgggcac gtgccaacgc aaggacggca agccgacact
    23221 gcacttgtcg gacaacgcca ccgacttctt cgagtccgtc gaggtgaagg tcgtcctcac
    23281 cgaagccgcc gatgccgcgg tctcggtgga catggacttc ggcgccgact cggagtcgat
    23341 cacccacacg ctcaccgcaa aaggcaacgg ccagggcggt acctcggcga agttgtcgcg
    23401 aacgggggag acgttcaagg tgaccggcac cggcaccatg accgatgacg gcaaggtgtc
    23461 ggccaatgcc gtgcccttcg cgatcaccgt gacctgcgcc agcgtcgact ggtagccaga
    23521 ccaacccgtc cgaccgattc gtcggtggcc gtccactctg cccgggggga ggggatgggc
    23581 cggctggctg tgtggttggt ggcgatcagc tcacctttgc ggtggcggcg cgcttctccg
    23641 ctgagttgaa accggtcgcg gagccgatca cggagaccgt gagcaccttg cccttgtcag
    23701 acttggtgat cttgtaggtg gacttggtcg cgcccttgat cctggagtcg ccgcggtacc
    23761 actggtactt gaagctgacc ttggccgcgg cccacgtgcc gggggatacg gtgaccttct
    23821 tgcccacctt cgccttgccg gacagcttcg gctcgggggc cgcgaactca cccttggcga
    23881 caccggcgag ttcggcggag acctgggtca ccgaggtgta gcccgcgcgg ctgccgacca
    23941 ccttgaccgt cagcttggca cccacgtcgc tggcctgcag gacgtacgtg cgctcggtcg
    24001 cacccttgat cgtggacttg ccgcggtacc actggtaggt gaaggccacc tcggcgggcg
    24061 cccagccatc cgtgaccgcg gtcacttcct ggccgaccgc gggagtgccg ctgatcaccg
    24121 gcgccggtgc ggcgctgaag gttccaggca cgacgactgc ggttgccgcg gacctgcggc
    24181 tgatcggggt gaacggagcg aggctgccag tgaccgcgac ggtgagcgcg ctacggatgt
    24241 ccgctgcttg cacctggtag gtcgctgcgt tggcgccggg aatggcggtg gcgtcgcgga
    24301 accactggta gctcagcgtc gcggacgcag cccagacgcc cgggtcagcg gtgaggatgc
    24361 cgtcaacgac cgcactgccg ctgatcgtcg gcaccggggc cggatcgacg gccatgaaga
    24421 gcttggccca ggtgttcgaa gtggccacgc ccgagggcga cagtccgacc gccgcttgga
    24481 aagccttcac tgccgcggtg gtcgtgctgc cgaaggtcga gccggagttg gtgcggtagc
    24541 cgaagtcctg gagcagggcg tgcagagctt tgacctgatc gcccttcgca ccggacttga
    24601 gctgaggagc gagtgtggtc agcgtgttcg ggccggcctc gccgtcgctt tcgagcttcc
    24661 tggccttctg gaacttcttc accgcagcct tggtcgcggc atcgtaggcg ccggagacct
    24721 tgacgctgac cctcgactgg cggaggaggt acttcagcgc ggtcaccttc gcggttccgc
    24781 tgccggagcc cagcaccacg gtgaacggca ccacgttgta gtcggcaagc ttgtagctgt
    24841 tgatcacgtc gcgcaggctc tgtgcgtagg ccgcggctgt gtccggacgg tcagccttgg
    24901 cgacttcgtc caccaacgcg tcccagttgt ccagaacgcc gcgggcggcc tcgtacttgg
    24961 cgccggtggt gatgatcctg gcccggtcgg cgaatgcgct gctggtggag gaatagttgc
    25021 tgtagcgcat cgtggttcca cccacggtga tcgccaggca gccgtactgg taggcgttcg
    25081 cggtggcgcc cttggtgact ccgttctcgt cggcgagggc gaagccgggg tagcggcgga
    25141 ggccggcgaa atacttgcgc tgcttccagt agctgagggt ggtcttgacc acgccatcgg
    25201 cccggccgcg ggcctcgacg atctcccact cacccgtgtc gagctggttg gtgaccaccg
    25261 cgacgtgacc gatgccattg gatcgggccg ggttgttgcg caggaacacc aggtcgccca
    25321 cctgcggggc gtcgatcgat accggcttgg tcacgttgta ctgcgaagcc gccaggtcgg
    25381 tgatgcggtt gccggagcgg ccgtagagcc actccaccag ttcggagcag tcgaacttgg
    25441 ccgggttgtt ctggctgatc gcggcttcgg ctcccagcac gtagggcttg ccgacctgcg
    25501 ccaccgccag cttcgcgaac tggctcgcgg tcatggtggc acggcactcg gtgttgaagt
    25561 agttccttgc ctgcttggcg gctgcagacg tgccccagtt cgagccgacg atcgcttcgg
    25621 ccatcagcac cgcggccggc acaccgtact ggcgctgcgc ggcctgggcc gaggtggcca
    25681 ggctggtgac gaaggtcttc ttggcgttgg ccgagatcgc ggacgcggtg gctggatcga
    25741 ccagggcgag cccgatcgac atggctaccg aaatggccac ggctaggagt ctcgaaacgg
    25801 gcttcacaag gggtttccta tccagcgact gtccagccgg acaagaccca ccttgagggt
    25861 tgagttagca gggccaactc tcaactaggc gctgactagg ggatttacag gcggcggcac
    25921 ctcaagtgca tgttcagggt tcgaggttga gggttgtggg ctgatacgtt cgcggcatca
    25981 cctggcggag ctcggagggg cgcacatgtc gcagccaatt cggatcggaa tcctcggtgc
    26041 ggcccggatc gcggagcggg cgctggtgcg tccggctgcg gctatcggcg ccgaggtggt
    26101 ggcggtcgca gcgcgcgatc tcggccgggc gcagcggtac gcgtccgagc acggcatcgc
    26161 ccgcgcctac ggtagctatg ccgaactgct ggccgaccct gaggtcgaag tggtctacaa
    26221 cccgcttccc aatagccagc atgcacccat gaaccaagcc gtgctgcgcg ccggcaagca
    26281 cgtgctcacc gagaagccgt tcgccagcaa cgcggtcgag gctcgcgagg tggccgccga
    26341 acctcgggac gggctggtgc tcttcgaggg cttccaccac cgctaccacc cgatgtacct
    26401 tcggctgctc caggtgatcg gcgatgggac gatcggcgag ctgaccgagt tgcgggtcaa
    26461 catgaagttc gactgcaccg acccgacgga catccgctgg tcgtggccgc tctccggcgg
    26521 ggcgcagatg gacctgggct gctacaccac gcacgtggcc cgtgacgtgg ccgcggttct
    26581 tggcggcggt gcgcggccgg tcagtgcggt agcgggcttc cgggaaggaa tcgacccgcg
    26641 ggtggacgct tcgctgttgg tgcgcgctga actgccaaac ggtgcgtccg ccgtgctgga
    26701 cgccgacctc ggtgctgccg ccgacatgtc gatcaccgcg accggcaccc ggggtacggc
    26761 ggtgctgccc aacttcatcg ccgccgatgc cgacgacagg ttgatcctga acgtggccgg
    26821 tgccgagacc gtcgagcacc acggcccgac cacgacgtac acctaccagc tgcaggcctt
    26881 gaccgcggcc atccgggatg gcgcggactt cccgaccggg gcggacgacg ccgtcgccaa
    26941 catggagttc gtcgacgagt gctatcgact ggccgggctg ccgccgcgcg agtccgcgca
    27001 gtgacggtct ctgcggcggt ccgagggtct ggaaacctcc ctgagggctg gtgaagaagg
    27061 accttcgcca agctcaggga ggtggctcag ggttcgatcg agtcggagat ctcgtccagg
    27121 gccggcaggc cgtggatcgg gtagctgacc ggacgggccg gcacgccggc gaccgtggtg
    27181 tagggcgcca cgtcctccag gaccactgaa ccggcgccga tcttggcgcc ctcaccgacc
    27241 cggatgttgc ccagcacctt ggcgcccgcg ccgatcatca cgcccttgcc gatcttggga
    27301 tggcgatccc caccgacctt cccggtgccg ccgagggtga cctcgtgcag catggagaaa
    27361 tcgtcctcga ccacggcggt ctcgccgatc accaccgagg tggcgtggtc gaacatgatg
    27421 cccttgccga tccgggcgcc ggggtggatg tcgacggcga agacctcgga gatccggctc
    27481 tgcaggtaga gcgcgagcgc ggaccgcccg tgcgtccagt agtagtgcgc cacccggtag
    27541 gactgcagcg cgtggaagcc cttgaagtag agcagcggga cggagtaccc gcggcaggca
    27601 gggtcacggc tgagcacagc tcgcaggtct tcgcgcacgg ccgcgccgat gctggggtcg
    27661 tcgtcgaagg cgccgatgat cagttcccgc aggctcagcg aggagatcgt ctcgctgccc
    27721 agcttgctgg ccaggatgta gctgagcgcg tcctccagcc gatcgtggca gagcacggtg
    27781 gtgtgcagga agctcgccag cgccggctcg gccaacgagt cggcggtggc ctcggtccgg
    27841 acccggttcc agacctcatc gcgcaggggg ttcatcggct acctctacct cttgtcactg
    27901 ctttcggtca gcacttccag cgccgccagc acctcagttt ttcgcggggc gccgacaatt
    27961 cgattgcgga ccgcgccggt gccatcgagc aacaacacgg tgggtgtccg ggtgattccc
    28021 aattcgttga ccagatcgag ccgggtggcg gcgtccagct cgatgtgctc gactccggaa
    28081 aggccggccg tgacttcggc gagcacgcgg gcggtcgctc ggcagggtgc gcacacctca
    28141 gcggagaact gcacgaacgt ggcctccgag ccgaggccgg tggccagtcg cgaagcgtcc
    28201 agagtcggtg ctgccggcga gccgaccacc tgcacccggc cgtcgatggc tttccggtac
    28261 aggccgaagc cgagcgcgac cagagcggcg atcgccgcga cgagcaaccc ggtcatgact
    28321 tactggacgc ggcagccggc cgcgcgaaca ggcggcgtcc gagcagataa agctcgcagc
    28381 ctaggcagaa gttgaacaca gcgttcaaga gcgcggccac cagcgcgaat ccgactgcga
    28441 tgaagaagac gaggtcgctg ctgaacaggg caccgagaat gccgaccagg gcgaacccca
    28501 ggccgactga ctgggcgaaa cgcggcgggc gctcgtcctc aagctcttcg ggtgcgccga
    28561 gcctcggccg tacgaagcgg cgaaacaccc agccgtacgg ttggtagcga agtccgagca
    28621 gtgcgcccag cccgaaagcg aaagcctgca ggaccagtag ggcgaggccc cacttcgggc
    28681 cggcgaccag ggcaagggtg agcactacgg aggtgacggt cgcgccgaag cgtggaccgc
    28741 gcggatcgat ctgtgaagcc atggaaagga ccttccggcg aagggagtct gtggggtgtg
    28801 gcggctcagg cggcgatgcc gaaagagctc gtgcagaagc ggtggaacgg gtgggacgcg
    28861 atcagccgcg acaacaacac cgacacatct cgatgcgcgc aacgctcagc gaagtggcga
    28921 gccggcgatc ggtgtgcatg cgaaaaggct agcacgcgtc cgttttgcgc ccccggcctc
    28981 aactgagtgg acggcttggc ggcgtccgga cgcgcaccgg tccaccttgt tggtggggcg
    29041 ggatgccgcg ggtcgcggtc gagtcagagt ggacgggttg gtggcgtctg agggaggaac
    29101 ggtccacctt ggttggcgca gcgccggcgg ggcactggct tgcggtcagg tacgctgaag
    29161 cggtgagttc cgtgcggcag tggctggcgc gacccggcct ggggtcgcgc gctttcgctg
    29221 cgctggccac gatcgcagcg ctctggccgg tgctgtccag cctcccgcgg gtctcgctgg
    29281 tcgcatgttt tgacgccgcg catccgctgt actcactggt gcctgccgag ttcggcgagt
    29341 tgcactgcct cagcgctcca gccccggtgg tggcctggac gttgatggtc ggggccacgc
    29401 tgatggtgca gctgctgttc ttgccgctgg tcgtgaccgc cggtgtggtc ggcggccgtg
    29461 cgctgcagcg gttggcgcgg tcagccaggc gtcagctcac gctggtgctg gtcgaactcg
    29521 gcgagatcgt ggttccgcag cgccgaccgg ctccagtccg cgtccgcgcc gcctacgccg
    29581 acgtgcacca cgcacgcgtg aatccgcacc gcggaccccc gtcctgcctt tcgtgaacct
    29641 gtgacccgag cgtgccacca ggcacggctc actagtgcgc ctgagggcgc ccggcggcct
    29701 tgatccgccc accttgaaac gtcacttggg ccatccgggc ccggaatcga aagcacagat
    29761 gtcaaaccgc aagaaccccc aggaacagtc caacgcccga accgcgctcc gccagcaaca
    29821 ggccgcacag gccaagggcg agcagcgtat gaagctggcc acccgcatcg cctggatcac
    29881 cggcctactg gtgatcgcct tgatgatcgg cgtgaccgtg tggtcggtca ccaactccag
    29941 aacggtcacg gcgacccccg ctccgggcgg cgtggtcacc ccgcgcaacg ctgccgaggg
    30001 cggcggcatc ctcgtcggtg acacagccgc tccggtcgcc gtcacggtgt acgccgactt
    30061 catgtgcccg tactgcgggc agttcgagca ggtgaacggt gcggatctgg ccgcggccgt
    30121 cgacgccggc agcgtcaagc tggagatgca cccgatgtcg ttcctcgacc agcagtcggg
    30181 aggcaccaag ttctccaccc gggcagccaa cgcctacgcc acagtggcca atcacgacgc
    30241 tgcgatcgcg ctcgacttca tgaagatcct gtatgacaac cagccggagg agggctcgac
    30301 cggtctgacc gacacccagc tggccgactt cgccaagcag gccggtgcgc ccgccgacgt
    30361 ggccgccagc ttcaccaagc agaccttcgt gccgtgggtg caacaggtga ccgagaacgc
    30421 gttcgcgtcc ggcatcgaga gcacgccgac ggtcaaggtc aacggagtca tccagaccga
    30481 gaacctgctg gtgcccggcg cgctgaaggc agcgatcgac aaggccgcca atggctgatg
    30541 ggccactgcg ccggctgagc acactgcgcc aatcggacgg gtggatctac accaccatgc
    30601 tggtctctgc gctgatcagc ctgaccgctt cgttcgtgct ctcggtggac gcgatcgcgc
    30661 tcgccgagaa cccgaacgcc gcgctcgcct gcaacatcaa cgccgtgctg agctgcggca
    30721 cggtcggggt ctcgtggcag gccagcctct tcgtgttccc gaacgccttc ctcgggctca
    30781 tcgctgagcc ggtggtgatc acgatcgcgg tggcgagcct cgggggcgtg cgtttcccgc
    30841 gctggttcat gtgctcagcc caggtggtct acaccctcgg cctggtcttc gcgtactggc
    30901 tgttcttcca ggcctacttc gtgatcggtg cgctgtgccc gtggtgcctg ctggtgacgt
    30961 tgtccaccac cttggtcttc acctcgctca cccacgtgaa cattcgggac ggcaacctgt
    31021 acctcccgcc gcgggcgaac cgggcgatgc agcgcttcgt gcgggcggat ctggacgcgc
    31081 tggtggtggc gatctggctg atggcggtcg tgatgctggt gatggtgaaa tatggtccga
    31141 tgctggtctg agcgtcggtg tgctgagaag gagtagcggt gtcgaaacag cccaagccca
    31201 gcgcgtcctc gcgccgggcc gccctgcgtg agcaacacgc gcacgaagcc gccaacgcga
    31261 atcgcaaacg gctgctggtg cgggtcgggg cggtcgtagc cgtggtggcc gcgctggtgg
    31321 cgggactcgc ggtgggagcg tacgtcaaca accggcccga cgactcggcg tccggtccgg
    31381 tggcgatcgg cgcgaccggc ccggcgaacg cgacgccgga cggggccgtc cgcatcggca
    31441 aggcggacgc gcccgtgacg gtctcggtct tcaccgactt catgtgtccc tactgcaaga
    31501 ccttcgatga ggcgaacgcg gaggcgctgt ccgcactgct gcagtcaggc aaggccaagg
    31561 tggacgtcta tccgttgacg ttccttgaca accagtccca gggcagccgg tactccaccc
    31621 gggcggccaa cgccttcgcg acgatcgcca acaccagccc cgaccaggcc tggagcttct
    31681 accagttgct cttcgcccac cagcccgccg agggcaccac cgggttgagc gatgtccagt
    31741 tggcgcagct gggtgaacag gccggtgcgt cgaaggaggt ggtcgcgtcc ttcggcaagc
    31801 ttgcctatcg aagctggacc gacgaagtca cccggcagtg gttccccaag cgggtgcaag
    31861 gaacgccgac catcaagctc aacggggtgg tgttcgacgg cgatccgtac accgccggcc
    31921 cgctggccga ggcgatccag aaggtgctcg gcgggtagac cgtcggggtt ggcggtgccc
    31981 tagcctttgg gcgtgacgtc agcagaatcc gggggcgccg cggccgggga gtcgcggcgg
    32041 cgaggatcct gctgctggtc gtcggtgtcg cactggcggc gaacgcgatc tggctggcgg
    32101 gcaccgccaa cctgacgctc ggcaccgcac tggtggcgct ggcctcgggc atcctgctcg
    32161 cgtggggaat ctggctgccc tggctgcgcg gcgtccggac gctgagcgtg gtagccgtgc
    32221 tgctggcgct cggcgtcctc ggactctcga gcttccttgc cgtccacggt tcggtcgaca
    32281 ccgccgacga tgatgcggac gcggtggtcg tgctcggcgc cgccgtccat ggccgcgaac
    32341 tgtcgcgcac cctggtcgga cgactggaca cggcgctggc ctaccaccag cgcaatccgc
    32401 gtgcagtgat cgtcgtctcg ggtgggcagg gaccgcagga ggaagttccg gaagcggtgg
    32461 cgatgcgcga ttacctggtg tcccgtgggg taaccgcgga cgccgttgtg ctggaggatc
    32521 gctcgacctc gaccgaggag aacttcgcct tctcaaaggt gttgctcgat cagcgcttcg
    32581 acgacgacta ccgggtggtg ttcgtcaccg acgagttcca cgtctaccgc gccggatgca
    32641 ccgccgagcg agccgggctg gcagcacagc acgtcagcag tcgcaccccg tggtacttct
    32701 ggccggccaa ctacctgcgc gaaaccgtcg cggtcgtcat ggacgcgttc ggcggctgag
    32761 ctcgtgggcc ttcctagcca cgcgcctgcg tcacccggcg cgggccggga tcggtccggt
    32821 gccgcacttg agatcgaagc accgacgtcc ttgaggtgcg ccagcaccag gcggacggcc
    32881 gcgcgggcca ggcgctctgg gcgtcccagg gcgacgatcg aacgcactga tcggacgtcg
    32941 gtcagcggga tcgtgaccag atcgggacgc gtccggtcat cggtgcccgg cggccgaagc
    33001 ctgcccgtca gcccgccgct gacgcggagg tcgagacggc gctcgccacc acctgacgac
    33061 tacagctgtt ccggatcctg gccctggttc ttgcggagtt cgtcctcgcg tcgcttgagg
    33121 tcgtcctccc actgcttgag cagccgctca tgctccgcgc tgcgcttgag tgaacggagg
    33181 aactccgggt cgtcgtccgg ggcgatcacg cgctgccgcg gcggctgttc gtagaggccg
    33241 gaggagggtg gccggggcgg ttgagcaggc gcggatccag tgggtcggcc cgcgaacagc
    33301 caggcgacgc agcccacgat gggaatcagg atcatgatca gcagccaggc ccagcgcggc
    33361 aggctgcgga tccggctctc gtcggcacgc aggcaatcga gcacgcagaa gatcaccaac
    33421 accaactcga cggcgaacgg cagtactcga atcattccaa ctcctggcta gtgcctcaac
    33481 gatatgtcat ggcaccagtc ccgaaatgcc gccggcttgc cccgccggtg cgctccgttt
    33541 tggcgccgcg accggtgggg tacgggggtt aggccgcgag ttgctgtgcc cgtaggtgcc
    33601 cggtgaacgc ttgggcgagc agccgtccgg tgcgggccac ctcgctgtcg tgggtctgca
    33661 tggagcggcg catggtgcgg gcatcgccgc catcgagagc cgcccagcgt cccatgagct
    33721 cgggcgatgc ctcgggatgg aactggacgc cgagcgccga tccgacccgg aacgcctggt
    33781 tcgggtagtc ccggctagcg gccagcgaca ctgagccttc cgggaggcgg gtgaccgcgt
    33841 cgtggtgcga agctgcgacc acggacctgc cgttcgccgc gagccggtcc agcagcgggt
    33901 cgtccgctgc ggccagatgc caatccactt cgaccgcgcc atgttcgccg ccaccagggt
    33961 ggttcaccgc cacttcgcca ccgaaggcct cggccagcag ttggtggccg aggcagatcg
    34021 ccagggtggg gatctcggcc tcgaccgctt cccggagcag gcgtttcagc ggctccatcc
    34081 aggcatgttg ggcgccatcg tgggcgctca tccgcccgcc cagcacgatc agcccgtcgc
    34141 cgagtgaatc cgcgtccggc acccccagct gccacagcgg aatggcgcgg acgaggacgc
    34201 ggttgccgct cagccaacgt ccgaaccggt cggccgggac gtctggatcg ggctggatga
    34261 tggtggcgcg cattgacctt ggcatgcgcc gcaggctatc cggcgattct gacggcgaag
    34321 ttacgagtct ggctggctgg gggagcggct cagcaatggt gcgcagcgtt ccgccggcta
    34381 cgcgaccggg acgggcgtcc gctcgaaatc cgccatcggg tcggcgatct caatcgtggc
    34441 gaccgggctg acgtggcgtg agaccgccag gctcaccacc agggcgacca gcaccaccag
    34501 caatgcgtgc cgggcgccgc cgaggtacgc ggcagcgaag ccgatcagcg gtgcggcggt
    34561 catcgcggcc accgagccca gcgaggcgac caccgagacc cgggccgcgg caacgtgcgg
    34621 ctcgtcggac gcggcggcta ccgcgatcgg gaagcagagc gcagccccga cgccccatag
    34681 gcagaccccg gccacggcgg tcgcgggccc gggggcgagg ccgaagatgg ccaacccggc
    34741 gaaggcgagg acgccggaga tctgcaggct gagcgtgcgg cccagccggt cgatcatgcc
    34801 ggtgccgaag gcgcgcacca gggtcatgga gccgatgaac gcgccgagta ccagcccgcc
    34861 ggacgactcg gccgtgccga acccgtccac gaacgcgagc gccagccagt tgttggccgc
    34921 gccctcgctg agcgcggcag caaaggcaac cgctccgatc agcagggtgc ggggttgtag
    34981 ccagacgccg agagccgacg gcccgcgatc cctggccggg ccggtcaagc gtgcctcggg
    35041 caggatcatg ccgtgctcga gcattgccaa tctcgcgcaa ccgactccca cagcggcgat
    35101 ggtgaactgc agccagaccg tcaggcccac cgacgatgcg acggcgccga gcagcgagcc
    35161 ggcgactgcg ccggcggaga aggcggcgtg gaagtggggg atgatcgtcc gcccgtaagc
    35221 ctgttcaagg cgtgccgact cgacgttcat cggcaggttg atcagcgctc caccgatgcc
    35281 gttgatcacg atgccggcaa ggaagagcgg acgggagtcg agcgtcggcc cgatgcccat
    35341 cagtgcgaaa ccggcggaca tcacgatcga cccggtggcg aacacccgtc cgctgccgaa
    35401 gcgggtgagc agggctgagg cggagaagac ggtgatcagt gcgccgacgg agccgaccag
    35461 gaggtagccg ccaagctcgg tggcggacag gtcgagtgca tcccggacac tagggatgcg
    35521 cgccaaccac gagaagacca gcagtccgct cagcgcaaac gcgcagagca gcagcaggcg
    35581 ggcacgatca agacggtttt cggcgatggc gagccggaac aagacagaac tccagggata
    35641 ggtgtgatca ggcgacttcc catccgcgga cttcggagca gggcgttggc ggacagccaa
    35701 caacgggcca gcaaggctgg ccgcggcttt ggggacgttt caatcccagc acttaaagcc
    35761 gccccggtca aaccggcgcg gcaactcatg gacgacaggt ttttggcgtg tgcggagatc
    35821 agatatagtt gaggcatcaa ctagctggag tactgatgac cctgactgct gaagctgcga
    35881 gcatctccgc cgacagcatc ttcgccgata tgcacaccgc ctaccggttc acctccgaac
    35941 cggtcaatga tgaggagctt gcccgcatct acgagctggc caagttcacc ccgaccgcgc
    36001 tgaacagcca gccgctgcga atcaccttcg tccgcacccc ggaggccaag gcacggctgc
    36061 tgccgctgtt gaacccgacc aaccggccca agtcggagtc tgcgccggtg gtggcgatcc
    36121 tcgccgctga caccgacttc cacgagcacc tgccggtgct caacccgcag gatgctggcg
    36181 ttcgggaatc gctcgctgag aacgccgaac gccgtgcggt gatggccacc aacaacgcct
    36241 ggctgcaggc tggcggcttc atcctcgcgg tgcgtgcggc cgggctggac gccggtccga
    36301 tgggcggcct caatgcagcc gcggtcgacg ccgagttctt ccccgggacg gcgttgcgca
    36361 ccctgctcgt ggtgaacatc ggccacgtgg ccccggacgg caacttcccg cgcaacccgc
    36421 ggctgggctt cgaccaggcg gtcacgctgg cttagccgct ggtgaccaat gctggctgag
    36481 cctgacgaag ccggctgtct gctgtgcgcg ggcttcggct ggtcgagtct gtcgagatcc
    36541 ccgccgacct gactcaggcc agcagccagc ggccgcccac caagagcgcg gcaatgctga
    36601 gcgcaagcca gccgagcgtc cagaacgccc gcggtacgtg ggtcaaccgg gccagctggg
    36661 ccgcgtcgga gttcgggctg cgattgccga gcagttcgat caccggacgc gggccggcga
    36721 ggaggagcgt ccaggtgagc aggccggcca gccaggccac gaagtcccca gggatgaacc
    36781 aggaggccag cgccaacagg gtggcggtaa gggccaccac cagggcgccg tagaagttgc
    36841 ggatcgacag cagcatcacc gccagaccgg cgaccgacag ccacagcacg cccgcggcgt
    36901 aaccggaagc gaccaaccag gctgcgccca agccgagcac cgaactcgcc ggatagccgg
    36961 cgagcagggt ggccgtcatg ccgaaaccgg tcggccgacc gcgggtcagg gtcagcccgg
    37021 aggtgtcgga gtgcacccga atcccgcgca gttggcgtcc ggtgagcagc gcgaccagcg
    37081 cgtgcccgcc ttcgtggcag atcgtgacca gtaggcgcac caccggccag gccagcagca
    37141 ccgccgcgag cgcatttccg gcaatgatca gcgtgatcgt gggctcgagc tgagcgctcc
    37201 tcgcggtcgc ccgcgcccac agttccgcga tcaggttcac ggacccagtc tgcaccgtgt
    37261 cgttgattcg cagctcgaag gtggggttgg gcgcgaaagg ccgacagttg cggcatcgac
    37321 caccgagatc gcgacatgcg gccgctggcg ggctgagggc tggatgtgag tcagcgctgc
    37381 cgggacacca gattgacgaa gacgtcctcg atcgtgggct cgacttcggc caccgtcgcg
    37441 cccgcgaagc cggcgaccgc gagttgttcg ccgagttcga cagcgctcag tcgtgacccg
    37501 gacgggacga cggcgtgcag ggaggcgccg gagaggtagg cctcggcgat ttcgggggac
    37561 tgctccagcc acagcagggc cgggtcggcg tcccgggtgg agacgtcgaa gatgcgctgg
    37621 tccatgtgct cggtgcgcag ctcagcgggg ctgccggacg cgatgatccg gccccggtag
    37681 atgaaggcca gtcgattgca gtgcgcggct tcgtccatgt agtgggtggt gacgaagagg
    37741 gtcaccccgc ggctggccag gtcgtagagc aggtcccaga attgacgtct ggctacggga
    37801 tccaccccgg acgtcggctc gtcgaggaag agcagttctg gctcgtggat cgtggctgca
    37861 cccagtgcca ggcgctgtcg ccagccgacc gagaggttgc gggtgagctc gtcctcgcgt
    37921 ccgaccaggt cggccatttt cagcacatag tcgcgctgag cggtgaactg ctgcgggctg
    37981 aggtcgtaga cgccggcata gaagcgcagg ttctcgtcga cggtcatgtc ctcgaagagg
    38041 gagaacttct gggacatgta gccgatccgc ggctggacgc gctccgggcg gcggatgacg
    38101 tcctcgccca gcaccaacgc gttgcccgaa ctcgaccgca gcgtgccggt gagcatccgg
    38161 atggtcgtcg tcttccccga gccgttcggg ccgaggaagc cgaaaacctg gccgcggggc
    38221 acctcgaagt cgatgttgtc gaccgcggtg aagtcgccga acctcttggt gaggccgctg
    38281 acggcgatcg cggggctggt ggcggcttcg ctgaggtcgg cgatcggccg ctctgagcgc
    38341 tgatctgctg gtcgcgcttg gcctgccctg agcttgtcaa aggatcgaga ccccatcatc
    38401 gccccttcct cactctgcga tcctgcggcg ggtggcgatc acggctgcgg agaagatgac
    38461 cacgccgaaa cctgccagcg cgagcaatgg caccgccagg gcttcgaaac tgctcccctt
    38521 gacgaaggcc ccgcgcagca cttccagggc gaacgtgagc gggatcaggt tggtcagcgg
    38581 ctggatgacc gcgggcatcg actggatcgg gaaaatgaag cccgagagga ccatcgtggg
    38641 gatcatcagg aacatcaccg cctgctgcgc ctggtgccgg gtgcgcgaga ccagggagat
    38701 cagcaaccca agcccgaggc aggtgaacat gaacagcagc aggccgacca gcactaccca
    38761 gaagtcaccg ttgaacggca cccggaacca gaagatgccg ccggcagcga ccagcagcaa
    38821 ctgcgcggtc gccagcgccg cgtacggcgt cagctttccg atcaggtact cggtggcctt
    38881 gatcggcgtc acgaacatct gttccagcgt gcccgactcc cgttccttca ccacggcctg
    38941 gctcatgatc accatcaacg aaatcatcat gatcgcagcc atcaatccgg ggatcatggt
    39001 gttgaccggg tcgagtgtcg ggttgaacat cacccggatg cgcgcgtcca cgcctggcgc
    39061 ggccaccgtc actccaagct cgtcgcggcg ggcggagttg aactgcgcca cgatctgcga
    39121 cgcgtaccca ctgccgatcg cggagatctg ggagtccgag ccgtccacga tgatgccgat
    39181 cggtgcggtc tctcccctgg tcagccggtc gtgcgtgccc tcggggatca ccagcgccac
    39241 cttcaccgtg cctcgatcga gcagcgggcg cagatcgtcc tcgctggccg ggtgctgggt
    39301 caccgtgaag taacccgagc cggtgaatgc cgcctccagt ttctgcgagg tggtcgagcg
    39361 gtcgaggtcg acgacagcgg tggtcaggtt gtgcacgtcc accgcgacta cgtagccgaa
    39421 catgatcagc tgcaccacgg gcatgaccag cagcagccgg atcagcatcg ggtctcggcg
    39481 cagctggatg aattccttcc agatcagcag gcgaatgcgc ttgatgctca tcgtgacgac
    39541 ctccgtccgt agagaacgga cgagatcacg atcatgacca gggcatagcc gctcagcagc
    39601 gccaactcgg gccagacctc ggtgaagccg gcccccttga ggaacacccc gcgggagatg
    39661 gtcaccatgt accgcgcggg gaaggcgtag ctgatccacg ccaaccaggt cgggatctga
    39721 tcgagtgcga aggcgaaccc ggacagcagg aaggacggca ggaaggcgac cagcatcgcg
    39781 atgatgttgg cggtgtcgag cgacggcgcg atggccgaga tcaacaggcc catgccaagg
    39841 gatgcgaaca cgaacaacgc cgcgccgacc gccagcgtcc agaagttgcc gcgtagtggt
    39901 acctcgaaga tggttcggcc gagaccggtg atcagggcga cgtcgaggaa cgcgatcagc
    39961 gtccacggca ggagtttgcc gaccatcagc tcgacccggc gcatgggact gatctcgagt
    40021 tgctcctgag tgccgaggtc gcgctcgcgg acgagggtga ctgcggtctg ctggaccgtc
    40081 acgatggcga tgatcacgat catcaggccg ggtatcagga agtccgagga gcggcgttcg
    40141 gggttgtacc aggtgcggac gcggggttcg agcatgccga gactggagag gtccaggccc
    40201 tgggcgtccg cccagtcgcg ggtcagttct tggctgtaga gctggttgag tgccaccgag
    40261 tacgcctggg acaccttggc cgcgttgggc tcgctgccat cgatgaggac ggcgacctga
    40321 gcctgttcac cggccgcgag gctcttggcg aagccggacg ggatgacgat cgccacccgg
    40381 gcgctgttgg catcgaaagc ggcgtcgacg tcagacattg aactaccgtg ttggacgacg
    40441 ttgaagaagg gcgacgaact gtacaagctg gcgtactcgc ggctctgcgt ggtctggtcg
    40501 aaatcgatca cgatggtggg cacatgctcg acgtcgaagc tgatcgcgta ggcgaacagc
    40561 agcagctgga ttaccggcgt gatcaccacc gcgagcaacg tacgggaatc gcggccgagg
    40621 tggcggaact ctttccggac gatcgcaccg attcgaacca acgcggcttt catgattcgc
    40681 caccctcaag gctcccggca gcagctggcg cgcgggtatc ggccaggaag gcgaacacgc
    40741 tctccatgtc catcgggatc ggccgcacgg ccacgtccgc gatagctgct tcggcgaggg
    40801 ccgcggccag cggctgcacc ggcgcgactg cgtccgacca cagcacacgc acgacatcgc
    40861 cgaacaggtg gggcgaagcg accccgggaa cgtcctgaag cgcggccagc gcggctctcg
    40921 gttcggcggc gacgacctcg accaactgcc ccggaacgcg ctgcttgatc tcttcgggcg
    40981 tcccggactc gccgatcgcg cccttgtcga tgaaggccac ctgcgtgcag cgttcggcct
    41041 cgtccatgta gggggtggcc acgacgaccg acttgccggc ccggtggagc ccggcgagga
    41101 tccgccagaa ctcgcggcgg gagaccgggt cgacgccggt ggtcggctcg tcgagcagca
    41161 gcaggtcggg ttcgtgcatc agcgtggtgg ccagcatcag cttctgcttc atgccaccgg
    41221 acaggaactg agcctgccga tcggtgaact cggccaggcc catgctctcg agcagcgcct
    41281 tcgagcgcgt ccggcggtcg gcgcgactca cgccgcgaat ggtggcgaag aagttgaggt
    41341 tctccgaaac tgagagatcc gggtacatcg agaagcgttg actcatgtag ccgatgcgcg
    41401 gtgtgaccag agcggcttcc cgggtcactg agtgcccgaa gacttcggca tccccggcgt
    41461 ccggggtgag cacggtggcc agcatccgga tcagggtcga cttgcccgcc ccgtccgggc
    41521 cgagcaggcc gaagaccgcg ccggcgggaa cgtcgaggtc gatgtcggcg atggccacgg
    41581 tctcaccgaa ggtgcgccgt agcccacgga tgcggacggc gagagtgtca tcgatcacgc
    41641 ccagatccgc cggtggacca gtggtactca tcaggcgaag gccacatcca ccggcatgcc
    41701 ggccttgagc gtgccggagg cgtcgttcag gcgtacgcgc acttcgtaga ccagcttgac
    41761 ccgctgttcc ttggtctgga cgttgttcgg cgtgaactcg gactcactgg cgatgaagct
    41821 caccgtgccc gagtagctgt cggcgctgga atcggtggtt accgtcgcag cttgaccaat
    41881 caagactctg ccgatctggg tctccggtac gaacacgcgg acgaagaggt cggctggatc
    41941 gaccagggtg agcagagtct tgccaggcgc ggcgttctgt ccggcgttgg tcgtcagaga
    42001 ggtgaccgtg ccggcgcggg gcgcggtgac cacggtgtag cccagttgga cctgggccag
    42061 cttcactgcg gcctcggcct gggccagtcg tgcctcggct gccttgacgt ctgccttggt
    42121 ggcatcgtcg tcgtcctttg cgttcgtcac cgcggccttg gcggccacca cgccctgctt
    42181 ggcctgctcc aattgcagct tgaacgccga gtcgtccaga cgcacgagct cgtcgtcctt
    42241 ggccacccgg tcgccctcgg caaccttgac ttcgatcacg cgtccggaca gcgcggaggc
    42301 gacctggtag gtgttggcct cgaccgcacc gctgagttgc gcctcggcac tcgcggcggg
    42361 ctgggtcgag ctccaccacc accagatgcc gccgccgatc agggcgagga ggacgactac
    42421 gatcaccgga atcggcgggt gcttgcgggt gtgtgcggcc atggctatct gccttcttcg
    42481 tggtcggctg cgaatcggca gccgttgatg gtgatctcga ccggcacgcc ggccggcaac
    42541 tgggcggtgg cggtgaactc cacctcgacc gccctggtca ggtggacctc ctcggtgggc
    42601 accgaggtcg gcgggtattc ggctgcggga gcgatccggg tgagcgtggc tggggtggcg
    42661 gcgttgcccc agtccccggc gacgctggct tcgtcgccca cgcagacctg gccgagctgg
    42721 acgggggaga gccaggcggt caccttgctt ggcgtggccg gacggatggc caccgcggtc
    42781 gcccccggcg ccagccggtc cccgctggcg gcaacgctca ccacgacccc gttcgcaggt
    42841 gcggtgagca cggcgagctt gaactgcacc tgcgcgagtt cgatcggcac ctgcatggtg
    42901 tcggcggcga tctcggcgag ttccttgagg tcgaccaggc cggcgcgggc atcggtgatc
    42961 ttgtcggctg catcagccag cttctcgagt ccgtctttgg ccttgcgtag ccccttttcg
    43021 agcttgggca ggttggtgtt gatcttctta aggcccgcgt ccatcttctt cagcgcggcc
    43081 ttcagcttgg cgatgccctg ctgcaactcc tcgcggctgg gtatgcctgg caccgggacg
    43141 ggcgggtagt tggccagcag ttcctgggcc tgcttgagtt gcttggccac tagtggccgc
    43201 tgcttcttca gctgcttgcg cgccttcacc aacttggcct tggtgtcggt gagggtgttg
    43261 atggccttct tcacatcggc cgtcttgtcg gcgacctctt cggccttgtc gtccgtcttg
    43321 tcgatggccg cggccagcag gtcgacttgg gcggcggtga ccttcgcgtc cgctttcgcg
    43381 acctgcacct gtgcttccaa cccggacgca tccagcgtgg ccagggtttg gccgactcgc
    43441 acggtgtcgc cctcggccac caggacgtcc gcgagctgta tgaacgagcc gagcgcgtag
    43501 gtggacgcgg tgcggctggc gtccgcggtg cgctggccgg acgattcgaa gccggcatca
    43561 aggttgaccg cgggcatcgc cagggtcggt accgcgacgg tctccaggcg atccttgacc
    43621 ctgccggcga ccaccagctc agcgggtgcc ggggcacagc cggcgaccag gagggtgccg
    43681 accagtacgg ctgcgaagcg cttcatgacc gcgtcacagt ggtcaggaag acatcgacca
    43741 gtttctccag ctcggctcgc ggcggcggcc cgctcggttc cacgctgagg aagccgtcca
    43801 gcgcgatgta ggtcatcatc gagccgacgt acatccgcgc ggacagctca ggatcgtcca
    43861 ggcggatcag gtccaactcg gcgctgtacc ggagcagctt ctcggtgcgg tcgagcacct
    43921 ggccgggcag cgcctcccgg aacccgctgc gtagctcggg cacgcggaag acctcgccca
    43981 gcacgagccg caccagggcg atcgactccg gctgtagcaa gtttgcggtg agccggacgg
    44041 agaactcgac caggttgcgc cgcaggtctt ccagggtgcg catccggtcc aggtctggtg
    44101 ggtccatcac cagctggctg atgccgttga ggatcacatc gccgagcagg tcgatcttgg
    44161 ttgggaagta ggcgtagagg gtctgcttgc tgaccgcggc ctcggcggtg accgcgtcca
    44221 tcgacgtggc tgcgaacccc tgggcgagga acagcttccg cgctgcggca ctgatctgtg
    44281 cgcgtttggc tgctctgcgc gggctcaacg cagtggtcat gaatcaaact gtaccgttca
    44341 gtttgattcg cgcaagtccc gtcgtcggcc ccttgtacgc tgggcgccat gacgaatccg
    44401 gtggccgagt tggcgcgacg gcgcaacgac ctacccggac ccgtcgatca ctcgctgcca
    44461 ctgccgaaac ggctgctcaa gtcgggctgg acctgggcga tcgtcgcgct caccctcgcc
    44521 tacaccgccg cgctgttctc gcagtacttc acgatcaccg cggacgtgcc ggtgaccggg
    44581 ggtgtgatcc caggcatcaa ttccgcggcc atccgtcgtg cagcctggct ggcgctgccg
    44641 acgctggcgt tctggacggt ggtcttcatc tgggccgacc ggtaccgtcc gcagcgcctc
    44701 gcggtctggt tcctgacgct cggctggggc gggagcatcg ccaccttcgt ctcgctgcac
    44761 atcaatacct gggccgccca gcagatggcg gtcaccggca acggcgaccc agccaccagc
    44821 gcccgcgcgg cgatcttcat cgcaccgttc gtggaggagg ccgcgaaggc gtccgtgctc
    44881 ttcctgatcg cggtgatcgc ccggtaccgg ctggtctcca aggtgtcggt gatcgtgctc
    44941 gcgggcttgt cagctgccgg tttcgcgttc accgagaaca tcatctacta cgccagggcg
    45001 atcgtttact ccagcgagaa catcggggtt ggcgacgcgg acgcggcggt caatcagatc
    45061 gtctggctgc gcggcttctg gacctgcttc gggcatccgc tgttcacgat gatgaccggc
    45121 atcggcgtgg cggtggcgct gcgcacccgc agcaaggtgg tgcgcgtgct cgccccggtg
    45181 atcggttacg gtgcggccgc cctgctgcac atgatcttca actcccaggc cagcctgaac
    45241 gaccagaacg gccagatgtt cgtgtacgcc ttcgtggcgc tgccgatggt gttgtccgca
    45301 gtgatctacg tggttcggca ggtgttcaag cagggccggg tgctcgccct gcgcttggac
    45361 gactacgtcc ggctcggttg gctgcccgcg tccgacccga tggttttcgc gaagctccgg
    45421 acgcggctgt gggccctgct ggtggcgatc acccgcggtt ggcgcacgtt caccgccacc
    45481 ctgcacttgc agcggacgct gaccgagctc gcctacctgc gcgacgggca gatcaggggc
    45541 gtttacgacg aggccggcaa cgtccgcgcc cgtgagctgg tggagactgc gcagagcctt
    45601 cggggcatcg ccatcgccga cccgagggga ctgaggctca acctgccacg catccgccgt
    45661 ccgcgggcgg tcgggtacgt gccgcccgcg taccccggtc cgtcaggaat cgggggcaac
    45721 tggccagcgc ggccgggtgc cgccccgcta ggatcgcctg ggtactcggc tgtggacccg
    45781 aagtggggtc cgccacctgg ttaggaggcg ggtatgagcg ctggacgctt ggctgcgtcc
    45841 ctcgtccgtc ttcgcgatgc cctcgccggc gtccgattgc cgctggagtt gcccggctcc
    45901 gcggacgcgc tggctcgtgc ccggggttat gtggcccaac tcgacgacta cgtgctgcct
    45961 cggctggacc ggctggacgc cccgctgctg gccgtgatcg gcggctcgac gggcgctggc
    46021 aagtccacgc tggtcaactc actgctcggc cgggtggtga ccgcgccggg cgtgatccgg
    46081 ccaaccaccc gctcgccggt actggtgcac caccccgacg atgcggagtg gttcgcgtcc
    46141 gacaagatcc tgcctgggct ggcccgcagc accaggaccg ccagcgacgc ccgcacgctg
    46201 caggtggtcg ccgaacccac cctgccgcgc gggctggcga tcctggacgc ccccgacgtc
    46261 gactcggtgg ttgccgaaaa tcgagcgctg gcggcccagc tcctggctgc cgccgacctc
    46321 tggctgtttg tgaccaccgc cgcccggtac gccgacgcgg tgccctggga ctacctgcgc
    46381 accgcggccg agcgccatgc cgtggtggcg gtggtgctcg accgcgtgcc gccggcggcg
    46441 atgggcgagg tgccggcgca cctcgggcag atgatgggcc agcgcgggct cggctcatcg
    46501 ccgttgttcg cggtgccgga gaccgacgtc gacgccaccg gactgctgcc gaacaccgcg
    46561 gtggcgccga tccgggactg gctgggcgag ctggccgcgg acgcggtgag tcgcaaccgg
    46621 gtggtgctgc agaccctgga cggtgcgatc ggagcgctcc tcgctggtgc gccgggaatc
    46681 gctgcggccg ctgaggcgca ggccgccagc ctggacacgc ttcgcagtga cgccgagaag
    46741 tcgttcgccg aagccgtccg gactatctcg gtgcagaccg cggacggcac cctgctgcgc
    46801 ggcgaagtgc tggcgcgctg gcacgacttc gtgggcacgg gggagttctt ccgtgctgtc
    46861 gagcagaaga ttggctggct gcgcgaccgc ctggtggctg cgatcaaggg cgagcccaag
    46921 caggctctcg acatgaaggc ggccgtcgaa tcgggcatgg aagtgctgat ccgcgaggag
    46981 ggcgaagctg ccgcgcggcg ggtcgagtcg gcctggcagg cgcatccggc cgggcgggag
    47041 ttgctggccg cgaccaagga ggacctcagt cgtgcctccc gggattatcc gctggccgcg
    47101 gctaacgccg tccgctcctg gcaaggcgat gtgcttgagt tggtgagtcg tgagggcgca
    47161 gcgaagaagt ccgcggcacg gttctgggcg ctcggcgtca acggggtcgg ggtggcgctg
    47221 atggttctgg tcttctccca gaccggtggg ctgatcggcg ccgaggtcgg ggtggccggc
    47281 ggcactgcgg tgttggccca gcgggtgctg gaagcgatct tcggtgagga cgcggtccgc
    47341 cggttggcca agcaggccaa aaccgatctg gacgctcggg tcgaggccgt gctggccgct
    47401 gagttgagcc gctatctcgc cgcggtggac ggggtggacg ccgcccccga ccaggccgag
    47461 cagttgctgg ccgcggtggc tgaggtggac gcgacccgcg cagccgacgg ccggctgacc
    47521 ttgccggggg agctggccct gtcggatcag ccggtctccc cagctcggct ggtcgagcgt
    47581 gcgcagaccc cgtcgttgtt ggatcggtcg actacagatc agccggtcga gcatgtggcc
    47641 ctgccgaacc cgtccgccac cgatcaggac attgcgatca ccgctgaagt cctcgccagt
    47701 gaggacgaac agtgagcgcg gccgacgatc atctcgccag acgggtccga gcgctcggcg
    47761 aggccgtcga tgcggcatcc ggacgcagcg acgcccaact ggtggccaag gcgaaggcca
    47821 tcgtcgccag ggcgggcgag cgggttgcgc tgagcggcag ctacacggtc gtggctttcg
    47881 ccggagcgac cggttcgggt aagtccagcc tcttcaacgc gatcaccgag actgaactgg
    47941 ctcgggtcgc ggtgcggcgg ccgaccacgt cgaagcccct ggcggtcagc tggggtaccg
    48001 agctgccgca cgagttgctg gactggctcg acgtgaccaa gcggcacctg gtgccgtccg
    48061 gcccgtcgga gttctccgac ctggtgctgt tggacctgcc cgaccacgac tcgaccgaac
    48121 tgggacaccg gctgacggta gaccggttgg ttgagctggt ggatgccctg atctgggtgg
    48181 tcgaccccca gaagtacgcg gacgcggccc tgcacgacgg ctatctcaaa cggctcgcgc
    48241 cgtacgcgga cgtgatgatg gtggtgctca accaggccga ccggctgccg ccggctgaac
    48301 tcgaccgttg cctgagcgac ctgcgacggc tgctggacag cgagggcctt cgggccacgc
    48361 cgatcatggc aacctcggcg ctgcggggca ccggcgtcgc ggagttgcgg tcggctttgg
    48421 cccgaacggt cgccaacaag caggccatga ccaagcggct ggctgccgat gtgagcgtca
    48481 cggccggcga gctggccgcg gaactcggcg cgtccgctga gccggacatc aacaaccggg
    48541 tgcgtcggca actggtgagc gtgctgggtg acgccgccgg cgtcccggtg gtggtggacg
    48601 gggtgcgtcg ggcctggcgc aagcgtgggg cggcggccac cggctggccc ttcgtcacct
    48661 ggattgcccg gctgaagccg gatccgctgc ggaagttgcg gctcgacctg ccgaagggcg
    48721 agcacagtcc cacggaggtg aatcggactt ccctgccgaa ggctacttcg gtgcagcgcg
    48781 cgcgggtcga ccaggcgttg cgcgagctgg tcgatgtggc atcggacggg ctcccgcgtg
    48841 gctggatgct cgcggtcaag ggggccgtac agggcaacga ggaaaagctg ctggacgacc
    48901 tggatcgggc catcgccggc gccgacctga cggtgcgcag gggcacctgg tggtgggccc
    48961 tgttcggggt gctgcagtgg ctgctcatcg ctgcggtggt cgcgggagca gcgtggctgt
    49021 tctccggtcc agtgctggcc agtatgggac taccgccgtt gccgacggtg ctgtggtact
    49081 cgattcctgc gggcacctgg ctgctggtcg ggggagtgct cggcggcatc ctgttggccg
    49141 tattggggcg ggcgttcgtc gagatgggcg ccaacagcca cgcccggacg gctcaacgca
    49201 gtctggacga ggcggtggcg gacgtggtgg ccagcgaggt gctggtcccg gtcgagtccg
    49261 agttggcccg ctacgcgact gcgcgcaaag cgctgcgcac cgcaatgggc tgagcccgtc
    49321 gactcttctg cccagttatc cacaggccca gattcatcca cagtcgacca aactcgctga
    49381 cgccgaaacc ggcctcctgc cgatagtgaa ggcagagagg aaggaagaac atggatgcag
    49441 tgatctggat gaccggctat gtcggcagtg aggtggagta ccggcaggtg cgggaagacc
    49501 tggtcttcgc cagcttccgg ctggcctgca cgccgcgact gcggcgcggc ggcgaatggt
    49561 ctgatggtga gacgacctgg atcggcgtca cgtgcagccg tgcgctggcg gagaacgttc
    49621 gcgacagtat cgccaagggc gatcccgcag tcgtggtggg caagctgcgc actagccgct
    49681 ggaaggacga ccagggggtc tcgcgggagc ggctggtgct tgaggccctc gcggtcgggc
    49741 acgacctcaa ccggggagtc acggagttca ccaagaacgt ccgaccgccg gtcgaagagg
    49801 ttcaggtgaa cctcggtgaa atggtgatgg ccaccgaact ccaggaggag gtctggtcgg
    49861 ctgaggccga ctcgcaagcg gatggtgtag ccgccgtggc ctgagactcg taccgattct
    49921 caccgtcgat tgcgctcgat caaccaatct ggatctcaac atgctcccgg tcaagtcagt
    49981 tcgatcggtt cggccctagg atcgttcgca tgggacggaa gggttcttac gcaaagggaa
    50041 tcgccaagcg cgaagagatc ctgacttccg ccctggaggt catcgcacgg gagggctacg
    50101 gccacgcgtc cctgcgcgaa ctgtccgatg cggttggtct cacccaggcc ggagtgctgc
    50161 actacttcga ctccaaggaa gacctgttcg tcgaggtgct tcggcgcagc gatcagctcg
    50221 cgtgggcccg ggtcgggcct ccgcaggggg ccgagactgc cgaacggttc ctggcgaacc
    50281 ttgcgggcaa caccaaggaa cccgggctga cccagctgta tgtgcagctt tccgcgatcg
    50341 cggcggcagg tgaggggccg gcgcgaacct acttggcgtc cagaaacagg gacttccacc
    50401 gctgggtcgc ggaatcgtta cggctcgaac gggaggctga cccgagcttc gcctcaggcc
    50461 tcgagccgct gctcgccact cgggtcatca tggctgtctc cgacggactg cagaaccagt
    50521 ggctgatgga cccgtccttc gacctggccg cgatcgtcgg tcacgtgatt gagctactgg
    50581 ttcgcggcgg agcggttgtg gacgaagccc gagacgtgcc ggaagaccat tcccgagcct
    50641 tggttgtccc ggctgtggcg cggctgaccg caccacgccc gcggccaaac accattccgg
    50701 cgtagagctg atctcgaagt gccgggcgcg cggcccagca gaccggtcga ttgaactgcc
    50761 gccggagacc gtttcgcgga tcccctgacg agttcagccg cgggtgagct tgcggtgcgc
    50821 ggtgcggtgc ggacgggcag cttcgactcc gagtcgggcg accttgttct cctcgtagtc
    50881 ggcgaagttg ccctcgtacc agaaccacac cgcgtcgtcc tcgtcggtgc cctcccacgc
    50941 gaggatgtgg gtcgccacgc ggtcgaggaa ccagcggtcg tgggagatca ccacggcgca
    51001 gccgggaaac tccagcagcg cgtcttcgag tgactgcagg gtttcgacgt cgaggtcgtt
    51061 ggtgggctcg tccagcagca gcacgttgcc gcccatcttc agggtgagtg cgaggttcag
    51121 tcggttgcgc tcaccgccgg agagcacccc agccagcttc tgctggtcgg gacccttgaa
    51181 cccgaaggac gcgacgtagg ctcgcgacgg catctcgaag ctcgcgacct tgatgtggtc
    51241 gagcccgtca gagacgacct cccagacgtt cttgcccgcg tcgatgccgg agcgaccctg
    51301 gtcgacgtag gagaacttga cggtgctgcc caccttgagt tcgccgctgt cgggctgctc
    51361 ttcgccgatg agcatcttga agagggtggt cttgcccacg ccgttggggc cgatgatgcc
    51421 gacgatgccg gcacgcggca gggtgaagct gaggtcctcg atcaacaccc ggtcgccgaa
    51481 gcccttggtg agcttcttcg cctcgagcac gtcggatccc agtcgcgggc ccggcgggat
    51541 gttgatctcg gccaggtcga cggcgcggtt gcggtcggcc tcggcggcga gctcctcgta
    51601 gcgagccagg cgggcacggc tcttcgcctg gcgggccttg gggttcgagc gcacccattc
    51661 cagttcgcgc tcaaggatct tggcgcgctt ggcgtccttc ttgccctcga tctgcaggcg
    51721 ggccttcttg gtctccaggt aggtggagta gttgccctcg taggggtgca gctggccgcg
    51781 gtcgatctcg cagatccact cggccacgtt gtccaggaag taccggtcgt gggtgaccgc
    51841 aagcaccgcg cccgggtagg tcttcaggtg cccctccagc cagttgacgc tctcggcgtc
    51901 caggtggttg gtcggctcgt cgagcagcag caggtcgggc tgctgcagca gcagcttgca
    51961 gagcgccacg cggcgccgct cgccacccga caacacgtcg acgatcgcgt ccggcggcgg
    52021 gcactgcagg gcgtccattg cctgatcaag ctgggagtcc agatcccacg cgttgcggtg
    52081 gtcgagttcg gtctggagtt cgcccatctc ggcgagtagt gcgtcgaagt cggcgtccgg
    52141 ctcggccatg gccagcgaga tctcgttgaa ccggtccagc atgcccttgg tctcggcgac
    52201 cccttcgtgg atgttctcca gcacggtctt gccttcggtg agcggcggct cctgctgcag
    52261 gatgccgacg gtcttgccct tgccgagcat cgcatcgccg ttgtcggcgt ggatcaggcc
    52321 ggccatcagc ttgagcatcg tcgacttgcc ggcaccgttc gggccgacca cgccgatctt
    52381 ggcgccctcg aagaacgaca gcgtgacgtt gtcgagcacc agcttctcgc ccagcttctt
    52441 gcggacgtta tgcatcgtga acacgaattc gggcatggcg atcaatcctt gcagagctgg
    52501 gtgtggagcg gcgtccagta tgccagcgtc cggaggcttt gggttaccgg tgggctgccg
    52561 gcagggtgag tagttcagcg agggtgagcg cgcgacggcc ggccaggtcg gcgagttgaa
    52621 gccggcagga gaagccgtcg gccagcagca ccgcgtccgg cccggcggcg gccaccgcgg
    52681 gcagcaggtg gttctgcgcg acggcggcgg acgtctcgta gtgcccgatc tccaccccga
    52741 agttgccggc gagcccgcag cagcccgaga ccgtgacaac ctgtgctcca gtgcgcgcga
    52801 gcagcgcggc gtcagcatcc cagccgagga cggacgagtg gtggcagtgc ggctgggcaa
    52861 cgaccaccgt gccgtggaga tcgggcggtg accattccgg gagggtggtg agcagttcgg
    52921 cgagcgtgtg gatgcccgcg gccacttcgg ccagccgaac gtcgaccacg agatcggccg
    52981 cgtcggaccg ccagaccgcg acgcaggacg ggtcgaggcc cacgatcggc gtgccagcgg
    53041 ccacgatcgg atgcagcaac tccaccgcat ggctcaacag ccgtgccgcc tcggcgcgct
    53101 ggccggtggt gatccaaggc agtccgcagc acgccgtccg ccgcaggatc gtcggcgcga
    53161 acccggcacc caccagcacg gccagcatgg cgtcgaggcg cgtcgcgtcc agggtgtcgg
    53221 tgaaggagtc cacccagacc actgcgcgcc gtccgctcac caatgggtct ggacgcgaac
    53281 gcgcgcgccg gtcggcgaac ttcggcagca gccgccgcga atccactccg gccagccact
    53341 tcgcagccgt tccaagcacc ggtgcggcga gagcggcgtt gacgaccgcc gccagacccg
    53401 gcaccgccgt gaccagccgg ccccagcgtg gcagccgtcc caacgcgtag tggctgcgcg
    53461 gacgcaggcg gtgccgataa gcctcggcca acacccgcga cttgtacgcg gctacgtcag
    53521 tgccggtggg gcactcggtg gcacaggcct tgcaggacag gcacagttcc agggcctcgt
    53581 gaacctccgg cgcgcgccag ccgccggtca ccagggtccc gttgaccatc tcctgcagcg
    53641 catgcgccct gcctcgggtc gagtcctgct cgcgtccggt cgcctggtag gacgggcaca
    53701 tcacgccgcc ggtgtccgct aggcacttgc ccaccccggt gcagcgatgg acgtcggcga
    53761 cgaacgtccg gtcgagcccg gacagtcgcg agcccgccgc ggcgggtagg cgcaggtcgg
    53821 cgtccattgg gcggggatcc accaggacgc cggggttgag caggttgtcc gggtcgaaga
    53881 tctgcttcac cgctgcgaac agcgccagcg cctcagtgga gtacatcttc ggcagcagtt
    53941 ccgagcgcgc gcggccgtcc ccatgctcgc cggaacacga tccaccgtag ccagcggcca
    54001 gttcggcggc ttcttccaca aaagcgcggt agcccgtcac accggtggcg ccggcgaggt
    54061 tgaagtcgat ccgtacgtgc acacagccct cgccgaagtg cccgtacggc aggccgtgaa
    54121 ggtcgtgtgc ggccagtagc cggtcgaagt cgcgcaggta ggcgccgagc acggccggtg
    54181 gcacggcggc gtcctcccag ccggaatggg ccggacgggc caaccccacg ctggccaacc
    54241 cggcaccgtc ggcccggata cgccacagcc tggctgcttc gtccgggtcg aaaatcagct
    54301 gggcggccag cgcaccggct gcggccacga gcttgccggc ccgctcggca agccgatcgg
    54361 cgtcggcgtc ggtgagttcg acgaagagcc agccgtcacc ggcggcaagg gtgggcaccg
    54421 ccgacgaccc gcgcaggcgt ccgaccacgt cgaggatgcg ccggtcgaag ccctccaccg
    54481 cggtgggccg gaactccagc acgacgggtg tggcgtcggc ggcgagcgcc atgtcggggt
    54541 agccgaggac caccaggacc cggtggggga ggtcttcgac gaggcgcagc cgggcgccgg
    54601 tgaccaccgc gagggtgccc tctgagccga cgaggaatcg cccgacatcg aacccgttct
    54661 cgggcagcag gtgctccagg ctgtaaccgg acacctgccg gggaaagcgg ccgaactggg
    54721 tgcggatgat cccgaggttg gcctgcacga gttcatgcag ggcggagagc gcgggggagt
    54781 tggcgtgcgc aaccgtgccg gacgcgaggc tgagccgctc gccggtgccg gtgatgacgt
    54841 ccagccccag cacattgtcg gccgtgcggc cgtacccgag cgcgcgcggg ccgcacgcgt
    54901 tgttgccgat catcccgccg atggtgcagc gatccgaggt ggacgggtcg gggccgaaac
    54961 gcaggccgtg ccgggccgcc gctgcggtca ggcgtgccgg gatcacgccc ggctccacga
    55021 tggccgcctt ggtgatcggg tccaggtggg cgatccggga cagccgggtg ctgtcgagga
    55081 tcaggcctgt gccgacggcg ttgccggcga tcgaggtgcc ggcgccacgg gtggttacag
    55141 gtagccccgc cgcgcgggcc gcgtccacta catcgacgag ttccggcacg ctggccgggc
    55201 gggacactgc ggcgggcggc acccgataga gcgatgcgtc agatgaatag agcgcccggg
    55261 tcagcgtccg cgagtcgacg ggccagccgg cgccggccag cgcagagacc tgttcggtga
    55321 accctgcggc gtccggcacc cggtcagtct gtcacgtcca ggacggcctg catggcccgc
    55381 cccagtgctg cgaagtcggc cgggtcgacg gggtctacca gcgcgcgccg aacggactcg
    55441 acatggtgcg gagccgccgc caccagcagc tgatggcccg cgtcagtgag taccgaaatc
    55501 accccgcgcc gatcggacgg acacgcggtg cggctgacga tgcccttggc ctccatacgc
    55561 gagatggtgt gggtgagccg ggagcgcgat tgccgcaccc gctcggccag gtcggacatg
    55621 cgcaacgcgt ggtccggaga ctccgacagg tgcacgagga tctcgtactc gcccagatcc
    55681 aggccgaatg gacgcaactc cgcctccagc gtctcgctga tcgcagccac accgttcagg
    55741 taggtgcgcc agatctgctg ctgctcggtg ctcagccatc gaggttggag ttgcgtggtc
    55801 atgggcgcca ctctatcaat gaagtttacg attcaacaat ccgctcaggt gcttcgagtc
    55861 agcggcggcc ccgtccagct tcccgccagc gaaactgaca cttgccgggg aacattcccc
    55921 ggcaaacgtc agtttcgctg gcgcgaatcg cgggagagcg tggaggctgc ttgcggcggg
    55981 tgggacgggg actgtggcgc ggcgaggtcc ttccagcaag gcgcaggcca ccctagactg
    56041 cacagacgat ccaacggtgg atgtgaggga gcaaagatga tcgacgtcga tgccatagtc
    56101 agcgcggcgc cacccacggt tggcgcgatg ttccgctggc gtgttgccaa gacgcccggc
    56161 aaggaggcct tccgctaccc ggacgcgaag gacaactggg tctctatcga ctgggccgag
    56221 accggacggc aggtcttcga cctcgccgcc gggctgctct ccctagggct ccagctcgag
    56281 gagcgggtcg cgatcgtcgc caacaccagg atcgagtgga tcctcgccga cttcgcgatc
    56341 aacgccgccg gcggcgcaac cacgaccgtc tacccgaata cccagggcgc cgatttcgca
    56401 cacatcgtca ccgactccgg atcggtgatc ctggtcgccg agaacgccga gcaggtggcg
    56461 aagctcgagt ccgccggcga tgctgccgcc caactgcggc acatcatcct gatggacggc
    56521 gaaggggacg gtgagcgcac gctcggctgg agccagcttg ccgagcgtgg ccgccagttg
    56581 ctcgcgtccg atcccggtgt ggtcgaccgg gcaatcgagt ccgccaccca cgacgggctg
    56641 gccaccttga tctacacctc gggcaccacc ggcatgccga agggtgtcga gctcagccat
    56701 cgttgctggg tttaccaggc gttcgtgctg gacgcgatgg agatcatcgc cgacaccgcc
    56761 gtccagtacc tgtggctgcc gctgtcccac gtcttcggca aggatctgct cacgatccag
    56821 ctcgcgatcg gcttcagcag cgcggtggac ggccgggtcg accacatcgt cagcggcctc
    56881 ggcgaggtga ggccaacctt catgtgcggt gcgccgcgca tcttcgaaaa ggtccgtgcc
    56941 gcggtgatgc tggcctcgcc gcgcggcggc atcaagggca agatcgcacg ctgggcgttc
    57001 gcggtcggcc gcgcgtcccg cgagtaccgc ctggccgaca agccgctacc accggccatg
    57061 cgattggctt ataccgtcgc cgaccacctc gtcttcagca agctgaagga tcgcgttggt
    57121 ggcaacatcg acttcttcat ctccggttcg gccaagctga acgcgcaggt gcaggcgtgg
    57181 ttctactccg ccggcctggt ggtcgccgag ggctacgggc tcaccgagac cggtgcggtc
    57241 agctgcgtga acctgccgct gccgccgcgg ttcgggacgg tcggtccggc gctgcccggc
    57301 acccagatcc ggatcgcgga cgacggcgag gtgctgatca agggcccggc ggtcatgcgc
    57361 ggctaccaca acgacccgga gcgcaccgcc gaagtgctgg tcgatggctg gttccacacc
    57421 ggcgatatcg gcaagctgga cgcggatggc ttcctctcga tcactgatcg caagaaggac
    57481 ctgatgaaga cctccggcgg caagtacgtc gcgccggcga aggtcgaggg tgtgatcgcg
    57541 gccaccattc cgtacgtttc gcaggtggtc gcggttgggg acggccgcaa gtacatctcg
    57601 gcgttgatca cgatggatcg cgacaacctt gccaagtggg ctgctcgcaa ggggctgacc
    57661 gaactgtcgt acgaggagct gacccagtcc gacgagctgc gtcgcaccat cgagcggttc
    57721 atccactcgg caaacgccaa gctggagcgc tgggagaccg tgaagcgctt cgccatcctg
    57781 ccggcagaat ttagcgtcga cgacggcggc gtcaccccga acatgaagat ccgccggaag
    57841 gtcgtcaccg agcagtacgc agacgtggtc gcgtccctct atgaagatga cgcggtcgac
    57901 gaggggtgag ttcgttccgc agccgcgtcc cgctgcgttg ggtggacctt gacgcgcagg
    57961 gccacgtcaa taacgcagtg atcgccgact acctgcagga ggcgcgggtc gagtggctgc
    58021 tggccggccc gaatgcacac ctgctgggct cggccaccat ggtggtctcc catcaggtgg
    58081 agtacctcgg gccggtgctg ttcagcctgg cgccggtaga ggtggtgctg cgggtcggca
    58141 aggtcggggc gtccaggttc agcctgggct actcagtgca gcaggacgga cgcgaggtgg
    58201 cccgagccaa ctcggtgctc tgcctgttcg actacgcggc cggccggccg cggcggatga
    58261 ccagcgcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    58321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncta cctcggccag
    58381 atcgcgcatc gtggcgaacc gtacctggta cgcacggggg tctgtcgagt cgggcggacg
    58441 tcggtgacga tcgctgcgca ggtcgaggac ccgcgcgcag tcggggtgct cgcccggacg
    58501 ctcacggtgc tggtcgccgg tgacgagcac ggtcgtccgg tgccactgcc cgcgtccatg
    58561 cacgcggctg ccgagcgctg gccggccgag ccggccccct gatggttggt gggcagcggg
    58621 tcgaggtcgc tttccgcacc ccgcgccgag acgagctggc catccgcgag cagtggctgg
    58681 cggatcccgg gttcatggcc tacaacgctg ggtgggccgt ggactttccc ggctaccacc
    58741 ccgacaccgg ctgcgtcgac ttcccgcccg atgcctggga cggctggtac gactactgga
    58801 tcggccgcgg cgagcgggac tactggttcg ttcaggacag ctcggggcag ctggtcgggc
    58861 acgcccacta ccgggtggaa ccggcggcgg acggacggcg ggtggcccac ctcggcgcgt
    58921 ccgtgcaccc ggaccaccgc ggacgcgggc tcgggtacgc cactttcgcc gaactcgtcc
    58981 ggcggatcga agcggccggc gtcgcggacg tggcccgcaa cgaactggat gccgggcggg
    59041 tcgccgccat tcggattcac cgagcgctcg gcttccgtcc aggctcggtc agcgatgagc
    59101 tcgacccggc ccgcccggtc accgtctggg agctcccgct ccaatcctga gcgcgccgcg
    59161 ccttcttggg cgcaggctgt ggatccgtcc accgtccgcg gtgcgctgag ccacagtcag
    59221 ggccggtgaa cgtggctgag cctgtcggag catggcccag tgggtgggcg tccggttggt
    59281 tgagcagcag tgactacgcg aaggttgagc ctatgcgcgc acgtcctggt cggttcggcc
    59341 gccggctcag ccaactgctg gtcggtctgg tgctgtacgg cgtctcgatg gcgatgttgg
    59401 tgcgcagcgg gctgggcgtc gacccatggg acgtcttcca ccagggcctg gccaaccatg
    59461 tgccgctcag tttcgggcag gtgaccatcg cggtgggcgc gttggtgctg ctgctgtgga
    59521 ttccacttcg gcagcggccc ggactcggca ccctgctgaa cgtgatcgtg atcgggctgg
    59581 ccgcggacgc cggcctggcg ctgatcccga tcgccgagag ctggcttgcg ggcacggggc
    59641 tgatgctcgg cggggtggcc gccaacggtc tcgccggcgc gctctacgtc ggtgcccggc
    59701 tcggccctgg gccgcgggac gggctctggg tggccctcac ccagcggacg gggcacagca
    59761 tccggctggt gcgcaccgtc ctggaagtga ccgtcgtggc cgtcggctgg ctgctcggcg
    59821 gcgtggtcgg catcggcacg gtcgtctacg cgctggcgat cggaccgctg gtgcagttgt
    59881 tcatgccctg ggttgaggtc cgcctcgatg agcccgaacg caacgatggc tgagcttgtc
    59941 gaagggtcgt ccattcgctt gtggctacgg gcttcgtcaa gctcagccag cgttggtccg
    60001 caatgatcag cggttcccta accgatcggc ctggtcgact tgtcgagacc cttcggcacc
    60061 cggatgggtt ttcgtcaggc tcagggatcg ttgacgctca gccgcgcttg aagtccgagg
    60121 aggcgcgcag caccagctcg ggttcgaaca ggctgtgtgg atgctcggtg agcagcaggt
    60181 cggctgcagc ccagcccagc tgacgcatcg gctggcgcac tgaggtgagt ggagtcatca
    60241 gttcggcggc gaagtagagg tcgtcgtagc cgaccactgc aagatcgtcc gggatccgca
    60301 ggccacgcgc acggatcgcg cgcatggcgc ccagcgcgac gatgtcgttc atgcagaaca
    60361 cagcggtcgg cttcgcgccg tcgtcgagga tgcggcccat gccctcctcg ccgtccgctg
    60421 cgttgaggtt ggacaggtgg atgacctgaa gcaccttcgc cggctccagg ccggcgttct
    60481 ggatcccctg caccgcgccc ttcagtcgat gccgggtctg ctgcagctca tcgcgtccgg
    60541 ccaggtacgc gatccggcgg tgaccctgcg cgagcagatg gccgatcgcc tgccgaccac
    60601 cggagacgtg gtcaacaccg acggcggcat ggtcgcggct gctcgcgtcc atcaacacgg
    60661 acgcgatccc gagctggcgc agtggggcca gccgttcctt ggtggtggac atcggcgtga
    60721 ccaggacgcc gcgcacgccc tgctcggcca gcagcctgag gaaccggccc tcacgttcgg
    60781 gatcctcgtc ggaggagcaa aggatcaggg tgtggtcatc ctgggccagc cgatcctcga
    60841 tgccgcgggc caactcggtg tagtaggggt tgccgaggtc catcacgatc gcgccaaccg
    60901 tccgcgaaac gccggcccgt agctgtcgtg cggaggcatt gcggtggaag ttcagctccg
    60961 ccatggcctg ttcgaccttg gtgcgggtct tctcggtgac cgactctggc ctgttcatca
    61021 cattcgacac ggtgccaacc gagactccgg cgagtgcggc gacgtccttg acgctggcgc
    61081 gctggcgctt catctgggcc tcctgccgtc ggtccactga gcatatccgt cagcagtcac
    61141 gccacacgct ggaatcgcgg gaatgtgtga atcgattcat gcaggcgctc ggtcagcgct
    61201 gataggtcac ccgaccgttc cagatcgtcg ccaccaccgg gtcgggcaga tcgaggccgt
    61261 ggtaggggtt gttccgagcc cgtgagcggg aagcgtcgcg gtcaaccgtg gcacgcgcgg
    61321 acgggtccac gagcacgagg ttcgccggct cacccactgc gagcgggcgc ccctggccgg
    61381 caaccttgcc gattcgtgcc ggcgcgtgcg acatccgttc gaccagggtc ggccagtcca
    61441 tgcgtccgct gttgaccatg acgtcgatga ccacggcgag cgcctgctcg agcccgagca
    61501 tgcccggcag cgcgacgtcg aagggatgat ccttgtcctg gcgtgcgtgt ggagcgtggt
    61561 cggtgccgac gatgtcgatc gtgccgtccg cgagcgcctc gcgcacggcc gccaggtgct
    61621 cggacgtgcg cagcggcggg ttcaccttga aggtggtgtc gtagccggcc accagtggag
    61681 tgtccagcag cagatggtgc ggggtggcct cagccgtcac cttgatgccg cgtttcttgg
    61741 cccagcggac cacgtccacg ctctcggcgg tggacacatg gcagacatgg acgcgtgagt
    61801 cggtcagttc ggccaactgc acatcgcggg ccacgatcac actctcggcc tgggccggcc
    61861 agccgggcag gcccagccgt cccgaccatt cggactcgtg gcagcaagcg cccggaccgg
    61921 ccagccgtgg atcctgggag tgctgcgcga ctacgccgtc gaacgccttc acatagctga
    61981 gcgcccggcg catcaacaac gaatcgtcca cgcagcggcc gtcgtcggag aacacggtca
    62041 ccccggcagc ggacgcgtgc atcaagccga gttcggcgag ttcctcgccg gccagccctt
    62101 tggtgaccgc gccgaccacg cgcacttcgc agtgtccatc gcttccgccg agggagtgga
    62161 tgtactcggc ggcctcagcg gtgtcggtga ccgggttggt gttggccatc gcgtgcacgc
    62221 aggtgtagcc gccgcgggcc gcagcagccg tgccggaggc gacggtctcg gcgtcctcgc
    62281 gtccgggctg gcgcaggtgg gtatgcaggt cgaccaggcc gggcagggcc agcagcccgt
    62341 ccgcgtcgat ccgctcgacc ccggccggcg cggacgcagg gtcggccagc cgcccgtccg
    62401 ccacgtacag gtcgccgcgg gtgccgtcgg cgagggttgc accggtgatc agcaggctca
    62461 cttcatcgct ccttcgttcg agccgagcag gtggtacaac acgctcatcc ggacggccac
    62521 gccggccgcc acctgttcga gcacgagcga gttggctgcg tcggcggctg cgctggagat
    62581 ctccacgccg cggttcatcg ggccgggatg gcagatcgcc gcgcccggct tgagcgcggc
    62641 cagacgctcc tcggtgaggc cgtaagcctc ggcgtattcg cgttcggtcg ggaagaaacc
    62701 gccggccatc cgttcacgct gcacccgcag catcatcacg atgtcggcgt ccgcgagcgc
    62761 ctcgtcgaag tcgctcgtga cggggcaacc ccaggtctcc actccggtcg gcagcagcgt
    62821 gggcggggcg accagggtca cctcgccgcc cagcgtccgc tgcagcagca cgttgctgcg
    62881 caccacccgg gagtgcagca ggtcgccgac gatcgcgatc ttcttgcccg cgaagccgcc
    62941 gctggtgtcg gtggagccga tcttgcggaa gtgcgcacgc atcgcgtacg cgtccaggag
    63001 cgcctgggtc gggtgctcgt gctgcccgtc gccggcgttg accacctgtg cgcgcaccca
    63061 ggacgcgacc tgggcggccg cacccgagga ggcgtgtcgg atcacgaagc aatccacgcc
    63121 catggcgtcg agggtgagca tcgtgtcccg cagcgattcg cccttggaga ccgacgaccc
    63181 cttcgccgag acgttgatgg tgtcggcgct gagccatttg ccggcgatct cgaaggaggt
    63241 gcgggtgcga gtcgagtctt cgaagaagat gttcacgatc gtgcggccgc gcagcgttgg
    63301 cagcttcttc accggacgac gctgcacgtc ggccatctcg gcggccacgt cgaggatcga
    63361 ggtggcctcg gccagggtca ggtcggcggc gctgagcaga tgcctcatcg ggtgcctcct
    63421 gggcgggaga tgaccacctc gtcgcgcccg tccaactcgt ccagcagtac ctggacgcgt
    63481 tcgtcggctg cggtctgcat cgcgcgcccg acgtggtcgg cggcgatcgg cagttcccga
    63541 tggccgcggt cgaccagcac agccagccgg accgcgcgcg ggcgaccgag gtcgcgtagg
    63601 gcgtcgatgg ccgcggacac cgtccgtccg gagtagagaa catcgtccac cagcaccact
    63661 acggtgtcat cgatccggcc cgggatggac gtccggccca ctctgcgggt ggggttggcg
    63721 cgaaggtcgt cgcggtacat ggtgatgtcg agcacgccga gccggacggg acggtcctcg
    63781 atcgcttcga tcagcttcgc caaacgcttc gccaggtgga cgccgcgggt ggggatgccc
    63841 aacagcacga gatcctcgca gccttggttg gcttcgagga tctggtgggc cattcgcgtc
    63901 agcgaacggg aaagctccgg ggcatcggca accactcggg aagcctcagc tggttgattg
    63961 gtcgcttcag ccacgcaagc cgctcctcag gtctcgaggc gttccgccga agcggacggg
    64021 ttctcgaacg gccaatctac cgattcctgg cgaccccggg ccgggcgtcc catcgaaagc
    64081 ctctgtcggc aggtggctcg cggcgggcag gaggtaccca acgaaaccgg cttcggctag
    64141 ctttggtgca tgccgctacc cgcactcgtt ccgctggaga cgctccccgg ttggcctgcc
    64201 gtgcaggagc cgaccgcgat tgaactactc accttaaccc tgttcattcc gctggcaatc
    64261 actgccgtgg tgatgctgct gacgatggcc cgcggctgga agtcgccgga tcgcggctga
    64321 cgagatcccg gcctgtgccg ggatgacgta gctgcctgcg ccggtcgcac tcatcaagct
    64381 ggacagttac gacccgtgac ggggcgcaac cgtccagctt gcgaggggag cgcgggcgat
    64441 gcgcggggta ggccgactca ggcgccggcg gcctgacaga acagcgcccg ctcggcgtca
    64501 tcgcgcttct cggcgaactg cctggccacg gcgtcagtta gcacggtgtt ggcccgccac
    64561 tggtcgatcc cgtccagggt cggacggtcg gccagcgggc tggggaacaa cagccccagc
    64621 acatgctgcc gaatcgcgga actgcgctcg ccccagccgt cacgtccatc agaaatcgcc
    64681 tccgcgacct ccaggtagcg ctgcacgtag ggccgcagca cctcgtcctg gccgaacttc
    64741 cagaagttct ggctgatctg gtagtgggtc tcgttggcca cgtcgggatc ggctgtgatc
    64801 agccgccaag cctcggtctt cgcctcggcc agcggttgcg ctgcacgtgc gccggcagcc
    64861 ttctcggcgc cggctgcggt cgcgtcgcgc tcggcttcag cgctgatctc ggcctcgccg
    64921 aacacgccga gccgggcgag gttggtcacg atcagccagc gcaggtcggt gtcgatcgcg
    64981 agccccgccg gtgcttcgtc cccgttcagc cacgccttga gcaggtcggc gacagcatcg
    65041 gagtccgcag cctggatcag ggctctggcg aacgccagct ggtgatccga acctggctcg
    65101 gcatccttga tcagccgggc caggcctgcg atcagacggt cgttcagctc ggcgcgagtg
    65161 tcccgcggtg cgtagtagtc gatcgcggtg cgcgactggc ccatcagcgc ctggacggcg
    65221 gtgaggtcgt cctcgacgcc ggcgccgctg agcaccagcg aaacgtaatc ggacgcgcgg
    65281 aactcaccat cgcgacacat gtcccaggcc gcaccccagc aaagggcgcg agccagtgcg
    65341 tcgtccagtc tgccgatgcc gctgaccagg gtggccaatg agcgctcgtc gagccggatc
    65401 ttggcgtagg tgaggtcgtc gtcgttcagc aggatcaggt cgggccgcgc ctgcccgatc
    65461 aactccggga tctcggtcaa ttcaccggcg atgtccacct cgacgcgatg ggtgcgggcc
    65521 agttttccgt ccgcctcggc aaagaggccg atcgccagcc ggtggtggcg cagggtcggc
    65581 cactgcggca ccgcggtctg ccggacggcg aagcggctga agttcccgtc cgcgtcggtt
    65641 tcgaactcgg cgcgcagcgt gttcacccct gaggtctgca gccactccgc ggcgaagtgg
    65701 gacaggtcgc gtccggacgc ggtggtgagt gcggccagca ggtcgctcag ctgggtgttg
    65761 ccccaggcgt gttcggcgaa gtaggcccga acgcccgcca gaaacgcttc ctgtccgacg
    65821 aacgcgacca gttggcgcag caccgacgcg cccttggcgt aggtgatgcc atcgaagttc
    65881 aactcgaccg cggccaggtc aaccatgtcg gcagccacgg gatgggtgga cggcaattgg
    65941 tcctgacggt aggcccaggt cttgcggctg ttggcgaagg tggcccacgg gttgtcgccg
    66001 gcgctgccct tggcgcggat ctcggcttgg cagaagtgcg aggcccattc ggcgaaagac
    66061 tcgttcaacc acaggtcatc ccaccaggtc atggtgacca ggtcgccgaa ccacatgtgc
    66121 gcgagctcat gcaggatggt gttgtctcgg gcctcatagg acgcgttggt cacccgcgag
    66181 cggtagaggt actcgtcgcg aatcgtgacg cagccggcgt tctccatggc gccggcgttg
    66241 aactcgggca cgaagatctg cgcgtagtcg gcgaacgggt acggcatccc gaagtgctcc
    66301 tcgaacaccg cgaagccggc tttggtggtg ctcaggatcc gctcggcgtc gaggtagggc
    66361 accagcgact gccgacagct caccgacagc gggatgtcct tctccctgcc cgcgtaggaa
    66421 tcggtgacag tgtggaacgg gccggcgatg atcgcggtga tgtaggtgga gatcggggga
    66481 gtcggcgcga acgtccagcg ggcaagggcg gacggctggt ccaacccctc cccgttctgc
    66541 tggtcgagcc cctcctgacc ttcgatcacc ggttccaccg cggcggagtt ggagatcacc
    66601 gtccagtcgg caggtgcgat cacggtgaac gcaaaggtgg ctttcaggtc gggctgctcg
    66661 aagcaggcga aggcgcggcg ggcgtccgcg gtttcgaact ggctgtagca gtagacgcgg
    66721 tcgtccacgg ggtcgacgaa gcggtgcagg ccttcgccgg tgcggctgta tcggcagagc
    66781 gcggacacgg tcacttcatg ctcgccggcg ctggcggcga accagacctt gtggtccacg
    66841 aacgcctcga cactgagtgg tgcgccgtcc agggtggccg ccagcacgcg gtcggcgatc
    66901 aggttgaggt aggcatcccc gccggtggtg ctgaagcgcg cggtcgtagt ggagacgaac
    66961 gtcgccgtcg ggtcgaacgg ttcggcgtcg ggcacgcgcc cggagaggtc gatggtgatg
    67021 tcgtagctgt gtgcggcgat ctggttggaa cgctcggcgg cttcggcgcg cgtgatgttt
    67081 gcggggtaca tctcacctca ctgcattggg gtggaagggc cggtcccggg tgcgggacgg
    67141 cttgtcggct gtagttcagc gcacaacata ctgcaacggt caatgcggcg agcgctggcc
    67201 gggtgcggcc agaagcgctt cggcggtctg ctcggccgca gcaaggtccg cagcggttgg
    67261 acgcgaaacg tcccgggccc gcattgcgcg agcgggatcg acgaggccgg agacgccgtc
    67321 gcggccgaaa ccccggtcgg gttcggggtg gcgcgccggt gtcgtcatgt tggcccaatg
    67381 tagactccgt tgccctgccg caccagggag tcgccgtgcc agaaggtcat gtgatccacc
    67441 gcctagccgg tgagctgaac gcggccttcg ccggacgcgg ggtacgggta accagcccgc
    67501 agggacggtt cgccggcgcg gctgagctgc tggacggccg tccgctgcgc ggtgctgagg
    67561 cgttcggcaa gaacctcttc ttggtgttcg gtgaggatgc ggtggtgcag atccacctcg
    67621 gactgatcgg caagctgcgc tgggtcgggc ccggggtggt gaccaatcca gacacggtcc
    67681 ggctgcgcat tgagaccgag gcggacgcgg tggagttgcg tggcccgcag acctgcgcgc
    67741 tgatcggatc ggtcgagatg gacgccgcgg tggccgccct gggtcccgat ccgctgcgcc
    67801 ccgacgccga cccggatcgc ggttggcagc gggtgcatcg ctcggcgcgc agcatcgcgt
    67861 ccctgttgat ggaccaacgg gtcgccgcag gggtgggcaa catctatcgg gccgaggtgc
    67921 tgttccgggc gcggttgcat ccggagacgc ccggcaacga actgtcgccg cggaagtggc
    67981 gtgaactttg ggccgacctg gtggaactga tgccgttcgg ggtgcgcgac gggcgcatcg
    68041 acacggtgcg cgacgaacac tccccggagg ccaccggacg cgaaccgcgg gtcgatcggc
    68101 acggtggcga ggtgtacgtc tatcggcgcg ccgggcttcc gtgcctggtt tgtgggcgcg
    68161 ccgtcagcgt ccgggagttc gagggccgca atctgtactg gtgcggaaaa tgccaacggc
    68221 ggcggtgatg gcttggggcg aagctgaggt tcggctcgct gcggatggga cgagcgcgag
    68281 gctgttcgcg aacggtgact tggtggtcga ggtgggttgg gtggacgcgg tcggcgccga
    68341 tcccgagctg gagcttgccg acgaggtcga gcacaccctg cagatccccg gcgggcggct
    68401 gttgcagcgg cagagcgttg cggacgcgtg gcgttcgcgc tgggtgctgc tgccggccag
    68461 cgatgtggcg ttgacggcga cgccgccgcg cgtccgggtg cgaccggggc cggagtacac
    68521 gctgtggagt tggggcagcg gggtgaccgc gctgctggcg gtgagcccgg cgcatgccgg
    68581 cggaccggtg cttgggttcc ggctggagca ggggtacctg gaggaggccg gcgatccgga
    68641 gtggagcagt gcgaccgccg agtacctggt ggcgccgcgg gggagcgagc tgagttctgg
    68701 gcagcggctg gtgacgtcgt tgcgggcgaa ctggttcgac cacttggacg atctggccgc
    68761 gaagctccca ccgtggctga acgacaccca gctcgacttc gaagaggagt ggtggggcga
    68821 cctggctgac ttcggcgtga ccgggccgga cgacgtccag atcgcgtacg ccgagggcat
    68881 ggtggtgatc gccggagagg gcggacggcg cgcgatcgac gtccagacgc cgcgcggctt
    68941 gagccgggtg ccagtggagt gggtgccgag cgtgcaggac gtgttgcatg ccgtcgcgaa
    69001 gacgaagctg agcctcagcc gtccgctgag cgcggcagag gcgttctgcg tgcagctcgc
    69061 ggctgaccag cacctgttgc ggctgaccga cgcgcacgaa gacctgctcg accaggtgga
    69121 ctgggccgcg gaggattcgg tgatcggagc tgcgtttgcg ctgctgcgcg gtcggaagct
    69181 cggcgaggct gcgttggtgt cggacgcgct gcggctgctg gctcggctgc cggttggggt
    69241 cggctacggc cggacggtga tggctggctg gctcgcgtcc atttcgttgg ggctggacgc
    69301 gcgggagcgc tgcttggagc tgctgggacg cagcgcggtc ggtcggacgg cttcgctgga
    69361 gagttcgctg ctgcactatc ggaacgccga gttcggggca gccgaactgg acggggtggc
    69421 gaaccgtctg ggcgggacgc ttcccggcga ggcgccgctg ctgacctgga ccgaacaggc
    69481 cgaactggtt ggcctcctgg aactctgtcc gccggagtgg cccggcgcgg cgcgatattc
    69541 gctggtggcc gagaaggccc gcggacaggt gctgtgcggc tattacgacg agcgcatcac
    69601 cgacgccgag cccctggcga tgctgttgct cagccccgaa ctgcgtgccg gcggctgagc
    69661 gacgtccctg agccccgttc caccgtcatc ccggcggagg ccgggatctc tcagcgcgat
    69721 cggggatctg tgctgaggcc tgtgggttgg tcgggtcatt cgactggtcg agctagagag
    69781 cggacg
//
LOCUS       DAOPRQ010000002        58179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_402_length_58179_cov_11.910330, whole genome shotgun sequence.
ACCESSION   DAOPRQ010000002 DAOPRQ010000000
VERSION     DAOPRQ010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 58179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 58179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58179
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_402_length_58179_cov_11.910330"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..780
                     /locus_tag="PKJ61_00325"
     CDS             <1..780
                     /locus_tag="PKJ61_00325"
                     /inference="COORDINATES: protein motif:HMM:NF017984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HOC12309.1"
                     /translation="ARQVEHDEQRTPGSWWNWSSVKSALEWLFYIGELSSAGRNTQFE
                     RRYDLPERVLPASVLATSTPSEEEARLILARRAAAALGVFTQKWLAEYFYTDRVGTIA
                     ALESLLESGEVEPVDVCGWSAPAYLWTQAARPRKLSVDALVAPFDTLVFDRDRLLDTF
                     DVHYRIGLYTPKADRVHGYYVYLFVMDDVIAARVDLKADRKAGRLLVQSAWLEDGAAA
                     AETGARLAAKLREMADWLGLTDVVVGETGTLAPTLKTAGRA"
     gene            complement(795..>1220)
                     /locus_tag="PKJ61_00330"
     CDS             complement(795..>1220)
                     /locus_tag="PKJ61_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020389467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC12310.1"
                     /translation="AIARALSMDPEMMLFDEPTSALDPELVGDVLSVMRDLAAGGMTM
                     MVVTHEMAFAREVADRVLFMDGGVVVEEGPPEEVIGNPKEERTRTFLARVLDPTHVRA
                     GAEDAAYAKRHAEDNGDPEGRGKRLPASHRPPSHRPNSH"
     assembly_gap    1221..1320
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1460..2290)
                     /locus_tag="PKJ61_00335"
     CDS             complement(1460..2290)
                     /locus_tag="PKJ61_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020389466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HOC12311.1"
                     /translation="MSTTVAPTTIPRKKLSPRKRAQRFRIAQYVILALVLLAVALLAD
                     GRQLQQVFFRPDMIAKTLGPNLLWALVNTILYTIGAFVFGLLLGTILALMKLSQIGPY
                     RWLATAYTEFFRGVPAIIVLLAFNLLPIAFPGLHLPWEPYGTVWTALGMVSAAYMSET
                     IRAGIQAVPKGQMEAARSLGMPAGMAMQRIILPQAFRIITPPLTNELVLLTKDSSLVY
                     VLGLTVSNFELTKYGRDLANSNGNLTPLIVAGLCYLIITLPLTYLVRRMEAQQRKATR
                     "
     gene            complement(2379..3200)
                     /locus_tag="PKJ61_00340"
     CDS             complement(2379..3200)
                     /locus_tag="PKJ61_00340"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOC12312.1"
                     /translation="MKRLMGLAAGAAALTLALTGCANYSQPATSAPTGGSAAAPALAS
                     AGKLTVCTHLAYKPFQFKDDAGKIVGFDVDLMDLVAKKLGVTQDIVDIDFGQITSGAV
                     FTAKKCDAGAAAITITDERNAAAPFSDPYFAATQALLVKTDSGIMGLEDLKGKILAVQ
                     TDTTGQKYAEDHKAEYGYEVKVYDDMPTAANAVLAGNAAANINDNGVVFDFAKDNPTT
                     TVVKEFETGEHYGFNTAKDNPAMLTAINDALKAAKADGTYNTIYKKWFGVDAPTE"
     gene            3399..6191
                     /gene="secA"
                     /locus_tag="PKJ61_00345"
     CDS             3399..6191
                     /gene="secA"
                     /locus_tag="PKJ61_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015070717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOC12313.1"
                     /translation="MDKMLHMGEGRQLKKLKVVADQVNSIEADFEAMSDEELRGQTAD
                     FRQRLENGESIDSIMPEAFATVREATKRVLGKRHFDVQLQGGAALHWCNIAEMKTGEG
                     KTLVGVLPSYLNALSGKGVHVVTVNDYLAKFQSEQMGRIHHFLGLKVGAILSQMTPAE
                     RREAYACDITYGTNNEFGFDYLRDNMALSLDECVQRGHHYAIVDEVDSILVDEARTPL
                     IISGPAEDNSSWYPEFAKLVRSLHRDEHYEVDEKKRTVSVLEPGITAVEDRLGIENLY
                     ESVNTPLISYLNNAIKAKELFKRDKDYVIMEGEVLIVDEHTGRTLIGRRYNEGLHQAL
                     EAKEGVEIKDEYQTLATITLQNYFRMYTKLAGMTGTAKTEESEFQKIYGLGVLPIETN
                     KPMIRVDQADLIYRTEEAKFAAIVTDVVPRHEAGQPILLGTASVAKSEVLSRLLKQAG
                     VKHEVLNAKNHEREAAIVAMAGRKGAVTVATNMAGRGTDIILGGNPEFLADAVLQGKG
                     LDPVEHADEYEAAWPETLKAIEQQVADEHDEVVALGGLYVVGSERHESRRIDNQLRGR
                     SGRQGDPGESRFYLSLGDDLMRLFKSDIIDWVLQTLKVPDDVPIENARVSKSVQQAQE
                     QVEAQNFEMRKNVLKYDDVMNRQRHAIYGDRRRVLEGADVEQQLRGTVDTVVEQYVRS
                     LTEGFVEDWDFDALWTQLHTLYPVTLSQADYEGRTDLTVDELVADFKADAQAAYDARE
                     AALGEDTMREFERQVLLTVLDRKWREHLYEMDYLREGIGLRAMAQRDPLVEYQREGGD
                     MFNTMMEAFMEEVVGYLYNLQVQVEPAPQVGLVAGADGVPITAEDLVPAASAPERKSS
                     MPAESSLTKRPALKAKGLESKAPRAMAYSAPTETGEVAKTGSAITDDPYAGVGRNAPC
                     PCGSGKKFKVCHGRTA"
     gene            6352..7764
                     /locus_tag="PKJ61_00350"
     CDS             6352..7764
                     /locus_tag="PKJ61_00350"
                     /inference="COORDINATES: protein motif:HMM:NF025670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC12314.1"
                     /translation="MSSARISVGLAVALAVLGCTGPVELVVAQEAALPQAPTTTAAAF
                     PDFDGDGTADLVYGVGTTRSYVALSYGSGRSQTITRTDAGGTDPASDDNTTGFGQGLL
                     ARNLNGDAYADLVVVDASQGAGSALYLVFGSADGLRPAAARRYPVPSGMYGFHGSPAL
                     LESPGRLLVVSTGVPGIKGGAVLAYQLGADGLPTGAPKLLSQQNLPGTDENGDAFGTS
                     IAASGNLLLIGIPGEDLDSVQRAGAVLVLKYGGGFKFTGTTITQRTAGVTGKAERWDG
                     FGRSVAIAGGYAAVGVPGEDLGKRDAGAVHVFKVTKGELKHVARVSQDSKWVSGKGET
                     GDMFGYSVAIGRTCDGYPGLLVGAPHEAIGSIAAAGSAWLIPIERHPGDCPVQLYEGG
                     RLGGTAREMASVGAAVSMLRSGKVGADTVVLTSPGMSEEGVYGRVITQATPFSGNAAT
                     VAKDLILNENSTLVLSPPAG"
     gene            complement(7745..8251)
                     /locus_tag="PKJ61_00355"
     CDS             complement(7745..8251)
                     /locus_tag="PKJ61_00355"
                     /inference="COORDINATES: protein motif:HMM:NF039460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rv3235 family protein"
                     /protein_id="HOC12315.1"
                     /translation="MHPSQAKCHAAPISRPPAIPLLVPAEYLGDTQPTLPWLDATEVV
                     QADSPVEPQLRQFASALMAAVVEVLSGQRSPLQLERWLDPELLALIEHLRGARLGSGL
                     RLQSIRVQAPNARVLEVSAHLRQGSASRAAALRVALRRDQWVGTHLSIALRPNVVHGA
                     GWISPLAG"
     gene            complement(8362..9663)
                     /locus_tag="PKJ61_00360"
     CDS             complement(8362..9663)
                     /locus_tag="PKJ61_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020390302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC12316.1"
                     /translation="MTLLLPLHGLGSRQDLPLPFPAVVGGAVVVLLVSFGVLAFAWRT
                     SRWSGHPGGRALPRLSRFIDSKWTRGLVAALGLFAFAWVGMALWFGQDRVTNPVFGFT
                     YVWVWVGLVPLSLLLGPVWRTLNPLRTLHRGMVHLGLPADGFRPLSASLWQRLGQYPA
                     ALALIGFGWLELVQPDNNTLAVLQGWWLAWVIGCLGGAVVFGERWLAASDPFEVYATT
                     VARASWWQRPLLVNPLRNLSTSTAPHGTWAVVTALLGITAFDGFTGTTWWVRTTQTSA
                     LPGTFWGTLGLLGMVALVGVTLALGARGLGRLTGRTFDRIAASVIPIVIGYAVAHYLS
                     LLVLEGQRTLINLSDPLGLGWNTFGTAELGINLFWLETPAITALIQLIAIVGGHVIGV
                     LVAHEVSLTLLPPKRRTSGQLAMLLVMIGYTCGGLVLLFSP"
     gene            complement(9660..10007)
                     /locus_tag="PKJ61_00365"
     CDS             complement(9660..10007)
                     /locus_tag="PKJ61_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC12317.1"
                     /translation="MKRLLVTWLALVLLSGCAAGASSTSAPPPPKQVTITLADGKAEP
                     IAEKVELVKGQHLVLTITSDRADAVHVHGFDVEIEVKAGETVTRDLVMDKVGRFEVES
                     HEPVFTLLQLQIQ"
     gene            10069..11328
                     /locus_tag="PKJ61_00370"
     CDS             10069..11328
                     /locus_tag="PKJ61_00370"
                     /inference="COORDINATES: protein motif:HMM:NF014998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0045017 - glycerolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HOC12318.1"
                     /translation="MSDPVSVTEAALLARDQESQPWQQAFVAMLSGPLTREQLIDRVT
                     ERIGYAPRFRRLVTGWPLPTWVDDVGFSVGGHVREATLAPGERLESWLSAKLQSGLDR
                     AHSPWEATLVHGIAPGTTALVVRTHAALVDGYDNVHLLQELFDEYAEDITDVDDAWQP
                     RAAEAFGVADVLSGLNDPLQALKSAATGLVGMVENAVRTASATARPQHLAGVEVDLDA
                     LAAVRERFGCTTHDVILALATAGVRGWMVEHNRELVDQVALVPLAVAEPAVLESAIGC
                     RVAPSWVALPIGVADAADRLRTLATLTRARIDTGVSVPARDLIDLAGFAPPTLHSVAS
                     GTVAAGRPHSVMVTNVPGPATRRYLGRARLSRVYAVSATTDSQDVSVSIVSYAGRVSF
                     GAVAVEPLGHWAADIDAELTALRGEAR"
     gene            11325..11792
                     /locus_tag="PKJ61_00375"
     CDS             11325..11792
                     /locus_tag="PKJ61_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC12319.1"
                     /translation="MTLVFVPVTVAELTDWATTGSLPGRRAAFATTPGLVAAFEVSDP
                     EEAEHIALLAASVAGLAASGTRLVAVAEASTTAGADSGADFGEVCVTDLPYRTVQSLF
                     ADEAAAEGLSEAAAAVRGADLETAWDHPAVVKVLRTAELLWHGAGEWSSLSTG"
     gene            11829..12629
                     /locus_tag="PKJ61_00380"
     CDS             11829..12629
                     /locus_tag="PKJ61_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HOC12320.1"
                     /translation="MPRSIWKGAVSFGLVTIPVKLYGATQEKDISFRQVHAADNGRIR
                     YKRVCEVCGEEVPYAEIAKGYEAADGRMAVLTDEDFADLPAAQGKAVEVVQFVDVDEI
                     DPTYFDRTYFLEPEKSGNKPYILLRQALAESGKAAVVKVALRSRESLALIRPVGEVLR
                     LHTMIWPDELRDDDFAAPTEEVKVNDAEVGMARMFIEQLSGKWDPDQFTDDYREALEK
                     VIEAKLEGVELPVEAEGTPSGAQVVDLVEALRASVEAAKARRAQAQAS"
     gene            12678..14312
                     /gene="pruA"
                     /locus_tag="PKJ61_00385"
     CDS             12678..14312
                     /gene="pruA"
                     /locus_tag="PKJ61_00385"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020390297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HOC12321.1"
                     /translation="MDAITFVPKPVNEPVLCYAPGSPERAGLKQTLATIATEPVDVFP
                     VIGGGWTPRADAATLEVRAPSDHSTLLARVRQASAADASAAIAAAMAARTAWSATSFD
                     ERAAVFLRAAELLAGPHRDLLNAATMLGQGKTAQQAEIDSACELIDFLRFNVAFAREL
                     YTNQPISSPGVWNRMDYRPLDGFVYAITPFNFTAIAGNLPTAPALMGNTVIWKPALTQ
                     TFAAQFWMQLLTEAGLPEGVINMLPGHGAEVSDAALNHHDLAGIHFTGSTRVFQGLWT
                     QVGANIANYGAYPRLVGETGGKDFVVAHPSADPAAVIAALVRGAFEYSGQKCSAASRA
                     YLPQSLWDTIKDELIAQVEALTVGDVRDFANFTSAVIDERAFDKLSGAIERARSSADA
                     EIVAGGTYDRSEGWYVRPTLITSDNPHQDIFTTEYFGPILGIHVYPDAKYDEVLQLVD
                     DTSTYALTGAIFATDRTAVAAASTALRHAAGNFYVNDKPTGAVVGQQPFGGSRASGTN
                     DKAGSLWNLMRWVSPRSIKENLLSPTTISYPHMLPE"
     gene            14320..15714
                     /locus_tag="PKJ61_00390"
     CDS             14320..15714
                     /locus_tag="PKJ61_00390"
                     /inference="COORDINATES: protein motif:HMM:NF005914.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOC12322.1"
                     /translation="MNQLLDPEIIEALRSEVHDALPRAVAELTDLVAIPSISFPAFDQ
                     SQLVRSAEAIRALATATGVFDEVAVHIAAIPGTSKHGQPAILARRAPAPGRKHVLLYA
                     HHDVQPPGAATLWDSPPFVATERNGRLYGRGAADDKGGVIAHLAALRALRQVLGDDLG
                     GLGVTLFIEGEEEYGSQSFGQFLSDHRDAMAADLIVVADSDNWNAQTPGITVSLRGNV
                     RFTLKVRTLAHALHSGMFGGAVPDAMLATIRLLDSLWDADGAVCVEGLATRDAATPAH
                     TEAALRESAELLPQTQPIGRGELLARIWNQPAITVTGIDYATVDEASNTLHPEVSVVL
                     SARVAPGQDPHEAYAAIQAHLLARAPFGAQLEFSDLDAGLGFLAAEGWATELGRAALA
                     AAFGAEPVDIGVGGSIPFISELAAEFPEAAILVTGVLDPTASPHSPNESLHLGTFEHA
                     ILAEALLLAGVAKA"
     gene            15837..16523
                     /locus_tag="PKJ61_00395"
     CDS             15837..16523
                     /locus_tag="PKJ61_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007077361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC12323.1"
                     /translation="MTLAIDPTREALLQRLRAQAEAPARSRAERCLPVPDAFAKLLPD
                     AGLKQGATYCLGVAGSLLTALLSEPSQAGSWCGVVGMPEFGVEAAQAAGIALDRLVLV
                     PDPGDQWLAVTAALAEVLPVIAVRPGGRVREGDAARLTARLRDRGGVLLVHGDWPRAE
                     ARLQLAEPRWAGLGIGHGCLTSREVVATATSKRFAGVRSVRLRLPDPQGRLALAAPVA
                     PVPVRLKAAG"
     gene            16604..18112
                     /locus_tag="PKJ61_00400"
     CDS             16604..18112
                     /locus_tag="PKJ61_00400"
                     /inference="COORDINATES: protein motif:HMM:NF013015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HOC12324.1"
                     /translation="MLWFPAWSVRAWSLAEGQPIDVPLATVAANRITACSAAAVAEGV
                     VIGQRRREAQSRCPTLQVIASDEARDVREFEPVVACIEELSPGVQVLRPGQCVVRARG
                     LANYLGSEAEAAETLLEAVVTRLGIPDGRVGIADGRFAAAQAARAGNPVRIVPAGESA
                     GFLAPLPISRLADEGLSTLLPRLGVHTLGDFAALSADSVRDRFGTQGLRLHTFASGLD
                     PSTVQPRTPPPELTRRLHFEPALNLVEQVAFSARATAEDFIAGLNAVGLVCTELRVEL
                     VGERDELVARNWLAAGVFDAGAVIDRVRWQLQAAAGRQLGSGVVNLLLEPIVVDAQGF
                     HMPGLFGSGPDERVHHVLSRVQGMLGHTGVLTAEVGGGRWLAERQVLVPWSDRPQSEA
                     PRQRPWPGRVPDPLPATVFSETRPVRLLGGSGRPVALNSRGAVTDPPVALEVEGGRRT
                     VIGWAGPWPIDERPWDADRHRVANRFQVVDGSGTAWLLLLDERGWWAEARYD"
     gene            18112..21510
                     /locus_tag="PKJ61_00405"
     CDS             18112..21510
                     /locus_tag="PKJ61_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020577745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HOC12325.1"
                     /translation="MGFNNPPMTWRTLERTLSAPGRTDAGQVEVPTSHKRGHYVPPPI
                     VRPDGGVPYAELHAHSSFSFLDGASGPAQLAEEAERLGLHALAVTDHDGFYGIVRFAE
                     AAESMQLKTVFGAELSLEQSAARTGQADPESTHLLVLAAGEEGYHRLASAITAAQLAG
                     GEKGRPYYQLDELAERAEGHWAVLTGCRKGVVRQALVDDGPAAAAAELDELVHLFGAE
                     QVFVELTDHGRPLDSTHNDVLAALAADRGLPTIATGNVHFATPAQHRLAQAVAAVRAN
                     RSLDELDGWLPAAGTAFLRSGDEMAARFVRYPGAVARTVSLADALAFPLRRAKPALPK
                     QDVPEGHTPMSWLRHLVWTAVPVRYPKLTEPQRERIERELAVIEAKDFPGYFLIVHDI
                     VAEARRLGILCQGRGSAANSAVCYLLNITAVDSIAYNLPFERFLSALRDEEPDIDVDF
                     DSDRREEIIQWVFDRYGRERAAQVCNVIQYRPKNAIRDISRALGYSPGQGDAFARQVE
                     RWGWLTTAEATNRELPEPVFELAQQLMGAPRHLGIHSGGMVLTERPVGEVVPIEHARM
                     EKRTVIQWDKDDAAWMGLVKFDLLGLGMLAAIQHCFNLIRDATGEAWELATIPKEEPG
                     VYDMLCRADSIGVFQVESRAQMGLLPRLQPRRFYDLVIQIALIRPGPIQGGAVHPFVR
                     RKLGREPVTYPHPLLEPVLARTLGVPVFQEQLMQMAMAVGECSGEDADLLRRAMGSKR
                     GVERIESLKAKLYAGMARNGLTGKAADQIYAQIQAFANFGFAESHSLSFALLVYASSW
                     IKLHYPAAFLAGLLRAQPMGFYSASTLTSDARRHGVRVLRPDLARSGVDAGLELSVAR
                     PVEPVETPSTGLTSCLNPEQPPVGEFDPTAPDESVSHRRDTNFAVRLGLAEVRGIGKD
                     VATRIVTEREVHGAYTSMPDLVRRTGLAVEQLEALAAADTFASLGLTRRQALWLAGSA
                     ALDQPDSLPGTLVETQPPLFGEPTPTEVLADDLWATGVSTTYHPIGQLRAELSARGVL
                     STAELATAEPGRRVQVGGLVTHRQRPGTASGITFVSIEDEFGLLNVVCSVGVWGRYRR
                     VARSARALVVRGKLERSPEGVVNLLADQLEDLPVLVPHKSRDFH"
     gene            complement(21575..21949)
                     /locus_tag="PKJ61_00410"
     CDS             complement(21575..21949)
                     /locus_tag="PKJ61_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004811985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOC12326.1"
                     /translation="MIEHLSLEDVLFLVDELGVGPVRDIGLLDSAVHRPGATVFGNEA
                     YPELDDKAAVLLESLVRNRALADGNRRLGWLAIVVFYGLNDIALDAPDDAAYDLVIAV
                     ASGAASYPEVAAQLAEWKRRTA"
     gene            complement(21946..22149)
                     /locus_tag="PKJ61_00415"
     CDS             complement(21946..22149)
                     /locus_tag="PKJ61_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HOC12327.1"
                     /translation="MAMTLRLTAEDERVLALLAESEGVSRQEATVRAIHEAAARRLRR
                     DRVAESSAAARDRYADLLDRLGK"
     gene            complement(22186..22548)
                     /locus_tag="PKJ61_00420"
     CDS             complement(22186..22548)
                     /locus_tag="PKJ61_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008356776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HOC12328.1"
                     /translation="MTHRVFRMSFASVYPLYVDKVERKGRTVKELDQVISWLTGYDAA
                     ELLQVIDTQVDLETFFAEAPAFNPNAALITGVICGYRVEQIEDPLMQKIRYLDKLVDE
                     LAKGKKMASILRGSEVNA"
     gene            complement(22593..24041)
                     /locus_tag="PKJ61_00425"
     CDS             complement(22593..24041)
                     /locus_tag="PKJ61_00425"
                     /inference="COORDINATES: protein motif:HMM:NF009709.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HOC12329.1"
                     /translation="MAKVSFYSGQQLPLEMHKVRIIQKLTLLPIEERGRAIAAAGYNT
                     FLLQNADVFLDMLTDSGVNAMSDQQQAAMLIADDAYAGSATYTRLYNKLVELFGMEYF
                     LPAHQGRAAEHILSQSLVRPGTIVPMNYHFTTSKAHVTAVGGEVLELITEEGLQVTSD
                     ELFKGNFDVPALRALIADKGPERIAYVRIEAGTNLIGGQPLSLANIRETTNVCREHGI
                     LSVLDASLLADNLHFNKTREPECSEMTIAEITRAIADCFDIIYFSARKLGFGRGGGIC
                     IRDEALYKQMRGLVPMYEGFLTYGGMSVREMEAITVGLDETMDEDMINQGPQFIEHMV
                     AELDARGIPVITPAGGLGAHLDAGRFLPHVRQQEYPAGALASALYLASGVRGMERGTL
                     SEQRDADGVEPLANMELVRLALPRRVFTLSQVNYAIDRIAWLYDHRDLIGGLRFIEEP
                     EILRFFYGRLEPIGSWVDDLVDAFRRDLGDSL"
     gene            complement(24137..24607)
                     /locus_tag="PKJ61_00430"
     CDS             complement(24137..24607)
                     /locus_tag="PKJ61_00430"
                     /inference="COORDINATES: protein motif:HMM:NF022161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2505 domain-containing protein"
                     /protein_id="HOC12330.1"
                     /translation="MEIHATNDFAATVDDVFAMLTDRVFLRAVCLASEPLDHEVSVDG
                     LRTRTRRVMPTPSVVGRLTGPTMAVIDEIAWDAPPVSGATAGTAHITVEGLPAELVGK
                     VRLVGGGKGAVLTYTGELTVRVPVLGSSLARQAAPLLLEALEIQQRVGDEFLAR"
     gene            24666..29372
                     /locus_tag="PKJ61_00435"
     CDS             24666..29372
                     /locus_tag="PKJ61_00435"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF016944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HOC12331.1"
                     /translation="MSTLVPGAQTGLRESLLTVADGDADLTALLRSYLAPVPEPDLVG
                     IEPDALAGLARAHLDMGRIRRPGEHLVTTFTPTEWDSHGSLVALLVSDDQRFLVDTVT
                     LELTRQEWSLRRLYHPQPRIRRDADGNLLGVGSGDVESWMALELYPPLGHSAEDLQPE
                     LESGLRTALATVTTTVADWQPMLARCREAVAELWADGSHRPTVSDGVDLLDWLAADHF
                     VFLGYARYVPAGDGLVPVAGTGLGLLRGVSEIDRLNVPRPGEDDPVVLMRDLRRSPVY
                     RRAYLTHLAVRLFGPDGALRTEHRFLGLLGASAYTEAVASIPVLAAKSQRLLADCGFE
                     PSSYGWNATRQVIATYPREELFEARVEELAPIVAAVAALRESRQTRVFLRRSRYGGFT
                     TALVYLPRDRYNTTTRLALQRILLDELGGLELEYNTLVSESVLVRLVFVVRMGPDAPT
                     HPDVDRIAMLAASATRSWGDDFTELAATLPSEQRGVEFGEVYEADHPPSVAMAHLHLA
                     NELTETDDLRVTIAEPPDPEDDPADLRLTVLSLRPMTLTEVMPHLSALGAQTVDERPY
                     EWQLRDRRVLVYSFGLRLGEGEQWDAGTRSRFTAAFVASWTGRCEADQLNQLVVPAGL
                     DWRQVSWLRALSRYLQQATLPFSQPYLATALTTYPRIAAALVKAFETKFDPAAFDAVD
                     ADAREAALAGLQQNLSEQLEQVASLDHDRILRAFWAVIRAGVRTNAFTDSATLALKLL
                     PAELDLLPQPRPEFEVYVYSPRVHGVHLRFGSVARGGLRWSDRREDFRTEVLGLVKAQ
                     SVKNTVIVPAGAKGGFVPQSLPTGRAEQLAEGRACYRLFIEALLSVTDNIVDGAVVPP
                     VGVVRYDGDDPYLVVAADKGTATFSDLANEVSVSRGFWLGDAFASGGTSGYDHKAMGI
                     TARGAWESVKHHFAELGLDPDTDPFTVVGIGDMAGDVFGNGMLLSRQLKLVAAFNHLH
                     IFIDPDPKPAASFVERDRLFRLPGSTWADYDPALISAGGGVFRRDLKSVPISRPMQVA
                     LGLSESVTKLTPNELISAVLRAPVDLLFNGGVGTFVKGSAETHTSAGDKANDGVRVNG
                     AELRARCVSEGGNLGFTQLGRVEYAENGGRINTDFIDNSAGVDTSDHEVNIKILLASE
                     LAAGRLTEPDRDMLLASMTDEVAGLVLAHNRDQNLALANAMFRRFDLAGQHEAWMTTL
                     EQYGLDRRLEALPSTHELNARLAAGRALTSPEFSVLLSYTKIALYRWVLASDLPEDPY
                     LADRLVQYFPQALRERYEAVMPQHRLAREIIATVAVNRFVDSQGITAYHRLSTETGAG
                     VADIMRAQLASRSIFNAGLDEVRIRRLVGLSADVGTELRVVLLRMVERATRWLLNHRH
                     GSLDITATVAEYGAEIAQLRPELLGLLSGRTAASSQAAEQRWLEAGLPEELARNLATA
                     GHAHTLLNVVDVAHRLDCDLLRAAQVHYRVAEALGIELLSSGVDVLPRQVRWDAMARA
                     ALRDELLTAHTDLTAAVLTHADAETDAATAVQTWLDAHPGVAPRVAMIRQVSDGTPDV
                     ARMNVGLSQLRAMLGG"
     gene            29369..30340
                     /locus_tag="PKJ61_00440"
     CDS             29369..30340
                     /locus_tag="PKJ61_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC12332.1"
                     /translation="MSPADPAGTRAEAVVDEWCAARLGSAPITAFFAARSMSSVRGVR
                     LADDRQVALKVRVASKRVLACAAGHRLAAAAGIDCPDLLAGPEWLPGLGWASAEAWRP
                     EGALRPSGDAAIAYADLLRRLVGALGDLDPGEFDPPPPWAHYDHGEPGRVWPAATSAG
                     WDPESPVVPDWVRRVAAAARERLLAEDHGAATLPAVVGHSDLNGLNVRWLDGPIVHDW
                     DSLAARPECVLAGIVAVNHVEGPNAGAIAAVESTEACLRHYQHDRQFSAVELELAWAA
                     GVWVAAYNAAFEYLHGRPGEVVRQLKADAAERLRRAGCSDCREPSHD"
     gene            complement(30432..31760)
                     /locus_tag="PKJ61_00445"
     CDS             complement(30432..31760)
                     /locus_tag="PKJ61_00445"
                     /inference="COORDINATES: protein motif:HMM:NF014741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HOC12333.1"
                     /translation="MEERTGLLPVGEALTQIRSVLDRVDPDRKALAPQVRLVAATVAR
                     SLTGRLDALASLLLAEADDAGASERTTGTPMSTWMAIDQNLTKREAAGALHRANQLAA
                     HPVLGEAAVSGRVSTGQVRAINTVLDGLTAQLDEQQQARAEQLLVGLADTLDSDALIK
                     SAPQVLAKVAPADANELLERKLQREAEAAHHNRSLRFWRQAGSVRFEGSLPRLAGEQF
                     ITLLDAHTEALRRTAVEARDPLFANATPEQRRADAFTHLLNHAATAKPKPGVGSAKVI
                     VKLDYNQLRNGAAGAGVVGDGNQLSAGELRQVCCDADLVPVVLRGGSEVLDVGRSRRL
                     VTPAMRAALIARDGGCVFPGCTAPPSACEAHHVVPWYLGGVTALSNLVLLCHHHHGLV
                     EPAKYYRRDQWQVRINQADRLPEFAPPSRHPQAGAWLRHTRHRHPERTAA"
     gene            complement(31875..32522)
                     /locus_tag="PKJ61_00450"
     CDS             complement(31875..32522)
                     /locus_tag="PKJ61_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HOC12334.1"
                     /translation="MTDPASDSGEPEAESAAGAPPHTAIEDLFGLLSGTFVASLGLFL
                     LHSVQAVTGGTAGLSLLLSYATGWPFGVLYLLTNLPFLALAVHRKGWNFALRTLVSVA
                     LVSAFASLHPLALPTLRIDPVYATLVGNLLAGVGMLMLFRHHSSLGGLNTLALILQEQ
                     FGWRAGYVQLAFDALIIASALTVVPVGNVLLSAAGAVVLNLVLALNHRPGRYLGH"
     gene            complement(32519..33586)
                     /gene="hisC"
                     /locus_tag="PKJ61_00455"
     CDS             complement(32519..33586)
                     /gene="hisC"
                     /locus_tag="PKJ61_00455"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008354646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOC12335.1"
                     /translation="MSEFPKSRPAVAALPAYIAGRKASGPDVAALASNESHFHPLPKV
                     LDAIATAADRVNRYPDMASTSLRDRIAVDAGITADAVALGPGSVGVLAQLVAAVCDPG
                     DEVVFAWRSFEAYPILCQIAGAVPIKVPLTTDESHDLPAMAAAITDRTKLVLLCSPNN
                     PTGTAISEGELVDFLERVPSHVLVVLDEAYREFVGESAPDVVQLIGSQYQNLCLLRTF
                     SKAYGLAGLRVGYAIASVEIATGLRKTQLPFSVSNVAEAAAVAALDSADEVGERVTTV
                     IAERERLLRSIRELGWVTPDSAANFVWLRCDEAERERLVAAFDAAGILVRGYPGDGVR
                     VTIADAVSNDRVLAVLAGFPR"
     gene            33707..34753
                     /locus_tag="PKJ61_00460"
     CDS             33707..34753
                     /locus_tag="PKJ61_00460"
                     /inference="COORDINATES: protein motif:HMM:NF021599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2332 domain-containing protein"
                     /protein_id="HOC12336.1"
                     /translation="MLADLMRDGIRVFGRRAPLYARLAAGFAGQPDVLAILAEAPAPQ
                     RIPVTLFAAIHDLLLADPEQELAQWYPNLTATPRTDDPLPAALAFCAEHRGDLVELVR
                     TRTPQTNEIGRCALLLVGLDRVATEVGALAQLDVGASAGLNLLADHYAYDYSGTRVGS
                     GRPLLSCEVRAAASDPLTGSAPARLPQITSRLGLDRNPIDLDDPEQVRWLEACVWPDQ
                     TERFERLRAAVEVAERLRPLVVRGDAVAGLAGTLEKLGEGHPVITSSWVLSYLEPERQ
                     QDFVAELNLLGSQQDLSWVSLEEPSAATDLGWPDALAGSSLSVLRLVRWRDGARVEEF
                     LAECHPHGWWLRWL"
     gene            complement(34865..35590)
                     /locus_tag="PKJ61_00465"
     CDS             complement(34865..35590)
                     /locus_tag="PKJ61_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HOC12337.1"
                     /translation="MDNSSGVGVLDKAAMVLTALEQGPTTLAGLVTATGLARPTAHRL
                     AVALEHHRLVSRDLQGRFVLGPRLTELASAAGEDRLLATAGPVLTRLRDITGESAQLF
                     RRQGDFRVCSAAAERPSGLRDTIPVGTQLTMAAGSAAQVLLAWEDPERIQRGLLNSSF
                     SAVALATVRRRGWAQSVAEREAGVASVSAPVRSPSGKVIAAVSVSGPIERLSRQPGRL
                     HAPAVIAAAERLSEVLRRNGGEG"
     gene            35667..37064
                     /gene="leuC"
                     /locus_tag="PKJ61_00470"
     CDS             35667..37064
                     /gene="leuC"
                     /locus_tag="PKJ61_00470"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HOC12338.1"
                     /translation="MGKTLSDKVWDNHVVRTAAGEPDLLYIDLHLVHEVTSPQAFDGL
                     RLAGRLVRRTELTMATEDHNTPTLNILAPIADPVSKLQVDTLRQNTDEFGVRRHSLGD
                     KDQGVVHIIGPQLGLTQPGLTVVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQTLMQ
                     ARPKTMAVNINGELPDGVTAKDVVLALIAKVGTGGGQGYVVEYRGTTIENLSMEGRMT
                     ICNMSIEWGAKAGMMAPDETTFTYLKGRPHAPDGAEWDAAVEYWRTLFTDADAVFDRE
                     VDIDATQLTPFVTWGTNPGQGVPLGAAVPSPEDFTDAVDKSAAERALAYMDLKAGTPM
                     RDIRVDTVFLGSCTNGRIEDLRLAASVLKGRKIADGLRMLVVPGSARVRLQAEAEGLD
                     KVFLDFGAEWRAAGCSMCLGMNPDQLAPGERSASTSNRNFEGRQGKGGRTHLVSPPVA
                     AATAIRGTLSSPADL"
     gene            37076..37681
                     /gene="leuD"
                     /locus_tag="PKJ61_00475"
     CDS             37076..37681
                     /gene="leuD"
                     /locus_tag="PKJ61_00475"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HOC12339.1"
                     /translation="MDKFSSHTGIAVPLRRSNVDTDQIIPAVYLKRVTRTGFEDGLFA
                     AWRNDPEFVLNDAAYAKGSILVAGPDFGTGSSREHAVWALQNYGFAVVVGSRFGDIFR
                     SNSGKAGLVIATVGQDVVEKLWEIAESEPGIEFTVDLRHRTIEAADNVFEFTIDDYTR
                     HRLLEGLDDIGLTLTHEDEIAAYEARRPSWLPKTLPVKTAG"
     gene            37878..38192
                     /locus_tag="PKJ61_00480"
     CDS             37878..38192
                     /locus_tag="PKJ61_00480"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HOC12340.1"
                     /translation="MVIEHAQISVIPGREDEFAANFDQALAVIRQAKGFRWAELHRFI
                     ERESVFLLLVGWESFEDHMTGFRESELFAQWRAVIGPFFAEPPVVEHFAAVTAGVGPV
                     AG"
     gene            38194..39297
                     /locus_tag="PKJ61_00485"
     CDS             38194..39297
                     /locus_tag="PKJ61_00485"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HOC12341.1"
                     /translation="MAAARTQVALVFGGVSAEHSISCLTAGSVARAIDTERFEVIGVG
                     ITRAGDWVRIAHAELVGYEVVDGQLPEVASDRPAAVLLAGGEVATRDGDRLLDRRGVD
                     VAFALLHGPYGEDGTIQGMFEMLGMRYVGAGVAASAIGMDKDLMKRAMAAAGLPVGPW
                     VAITPSQWRDQREASLARINDLTYPLYVKPARGGSSLGIVRVESADELVAAIEVAQGF
                     DPKVVVEQGFVGVREIEVSVLQDLDGPPRVSLPGEILMHTPDAFYDFEAKYLPEEQVT
                     LQVPADLPDDLRTAVQELAARTFSAMDSEGLARVDLFVDGSGKPFVNELNTMPGFTRH
                     SMFPMMWAATGVEYPELIATLVELALRRPLGLR"
     gene            39294..40244
                     /locus_tag="PKJ61_00490"
     CDS             39294..40244
                     /locus_tag="PKJ61_00490"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012922397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HOC12342.1"
                     /translation="MTIGGLGEFALIEQLTSGLTASGAVRLGVGDDCAVLRLAGDLAV
                     STDTMVEHVHFRRDWSSAHDVGRKAVAACVADAEAMGAVPLGVVISLAAPAELPVEWA
                     AEFSAGVQAECANAGAQLVGGDTTSAPLIVITATVLADLGGLPPVTRAGARPGQVVAV
                     AGRMGWAAAGLAVLARGFRSPRAVVVAHRVPEPPYGQGRVAAEAGASAMIDVSDGLLA
                     DLGHVARASGVTIELDTSTLEISEPQQAVAAAIGGGDPLVFQLTGGDDHALAAIFEAG
                     SVPTDWRVIGRVREGDPQVLVDGRTWAGPTRGWQHFAPLP"
     gene            40436..41857
                     /locus_tag="PKJ61_00495"
     CDS             40436..41857
                     /locus_tag="PKJ61_00495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOC12343.1"
                     /translation="MTKDLTVGPPTRMILWFTLPLLVGNLFQQLYAVVDAIVVGRLLG
                     VEALASAGASGSLHFLLFGFAIGTASGLAIPIARAFGAGDLAGMRRAVTTGVVISGLV
                     TVVISLVGVFASRTLLTLMGTPPELLDDAVSFLTVFFSGTVATVAFNYLAAVVRALGD
                     SRTPLYFLILACLLNVGLVVLFIGGLHFGVGGAAAATVLAQFVSVVLCLVLIVRRMPV
                     LRLHADDWRLSRSQLAESSRLGLTMGFQMSVIAVGAVILQSGINGLGATAVAAFTAAM
                     RVDQVAVAPLASFGVAIATYVAQNRGGREWHRIRVGVFRTGLVAVGVSLLLGVVIISF
                     GTAIVGLFVGDSAPEVVAMAHRYLVINAGLYAILAVLFVLRNALQGMGSATVPTVAGF
                     MELLLRSLAGLVLVAEFGFLGACLAAPLAWIGALVPLAVAWLIERRRLIDREAGIAPA
                     REPIPDWPELDDVLTPAGALAAD"
     gene            complement(41909..42070)
                     /locus_tag="PKJ61_00500"
     CDS             complement(41909..42070)
                     /locus_tag="PKJ61_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC12344.1"
                     /translation="MNRTKPDGCGCLLAGLRVLLDLLPLLRLEALGREVALLRLREGE
                     DARVAMVEG"
     gene            42069..44342
                     /locus_tag="PKJ61_00505"
     CDS             42069..44342
                     /locus_tag="PKJ61_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HOC12345.1"
                     /translation="MSGLGRGIAAGWRGLATMFGSAARGIGPKVVLEPDQRHDGTALF
                     LLLTGLLVAAEFWVGLPAVLGDWVHAGTTTLVGTFAYAVPVLCWAVAWRTWRHPERHG
                     TAGRQAVGWTAGLFGILGLVHIAKGLPRGSQPALVREAGGFLGFISSSLTVDLLTVWL
                     AVPLLVIVTTFGVLVVLGVPMSELKSRFNAARERLKRPERPEIAYGAAHRAFDTPLVD
                     EPELDDDEVLTEPISVSAAAPKPRLKPVQPPLEAPVISSGPLRAEQPPISSDIAYVLP
                     DAGLLKAGSVPKARTQASDAVVNSLTEVLRQFEIDATVTGYTRGPTVTRYEVELGQAV
                     KVEKVTALGKNIAYAVASADVRILSPIPGKSAIGIEIPNADKEIVSLGDVLRSNRARN
                     DHHPMTVALGKDVEGGYVVANMAKMPHLLVAGATGSGKSSFVNSMITSIMMRATPDEV
                     RMLLVDPKRVELTHYEGIPHLVTPIITSPKKAAEALQWVVREMDQRYDDLAAFGFRHV
                     DDFNKAVRANQVKLPPGSERVLAPYPYLLVIVDELADLMMVAPRDVEDSIVRITQLAR
                     AAGIHLVLATQRPSTDVVTGLIKANVPSRLAFATSSMTDSRVILDTPGAEKLVGQGDG
                     LFLPMGQSKPLRVQGAWVTEAEIRAVVKMVTEQMAPQYREDVAAPAESSKAVAEDIGD
                     DLELVLEAAQLVVNLQLGSTSMLQRKLRVGFAKAGRLMDILETRGVVGPSEGSKPREV
                     LVKPDDLDEVLAALRAG"
     gene            44448..45833
                     /gene="rimO"
                     /locus_tag="PKJ61_00510"
     CDS             44448..45833
                     /gene="rimO"
                     /locus_tag="PKJ61_00510"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015582831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HOC12346.1"
                     /translation="MTSTPVHLISLGCARNEVDSEELAGRLAAGGFSLVDEADDATAI
                     VVNTCGFIDAAKKDSIDTLLAAAERKPDGVRAVVAVGCLAERYGAELARELPEADAVL
                     GFDDYPDIAERLRWILDGGRPEAHEPRDRRKLLPVTPVARPARAVDVAVPGHGWIPRF
                     RLAKGASAPLKIASGCDRRCAFCAIPMFRGSYLSRPVAEVVTEAQWLVENGVREAVLV
                     SENSSAYGKDIGSNLEALLTELDGVSGLDWIRVSYLQPAEVRPSLLEAMQGLESVVPY
                     FDLPFQHASATVLRRMRRFGDPDAFADLLARIRADRPDAGIRSNVIVGFPGETEDDVA
                     ILLDFLASARLDAVGVFGYSDEEGTEGAGLSGKLPEDVIAERVDRVASLVDEVSAERA
                     SERIGERVQVLVESAIGSSGWLGRTAQQGPEADGSITLSASVGQLTPGTLVWSSVTAT
                     DGVDLVGEVVA"
     gene            45834..46445
                     /gene="pgsA"
                     /locus_tag="PKJ61_00515"
     CDS             45834..46445
                     /gene="pgsA"
                     /locus_tag="PKJ61_00515"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015070239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HOC12347.1"
                     /translation="MTERSADARTSIPAWLPNALTVVRLILVPVFVVLLFLGPASFAW
                     RMAATVAFGLAILTDYFDGNLARKHNLVSDFGKIWDPIADKALTGAAFVSLSLLNELP
                     WWVTVIILVREWGITWMRVAMLKYGVMAAAAGGKLKTLLQAIALLMFLPYPIGQTMAI
                     APFWGWLAWLVMGAAFVLTAVTGVMYVFDALRLKAESTAGSKR"
     gene            46442..46927
                     /locus_tag="PKJ61_00520"
     CDS             46442..46927
                     /locus_tag="PKJ61_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017935194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HOC12348.1"
                     /translation="MSPNPSASGVVAALTARGQTLATAESLTGGLIGAAITSVPGASA
                     IYLGGVISYATTMKAVLAGVPGAVLRTHGPVAAETAVALAVGVRERTGADWALAATGV
                     AGPDPQDGHAPGEVWLGLAAPDGAAIGRRLDCAGAREDVRAATVIAALELLLHELQLE
                     A"
     gene            47034..47339
                     /locus_tag="PKJ61_00525"
     CDS             47034..47339
                     /locus_tag="PKJ61_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008357297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOC12349.1"
                     /translation="MIQEVRERPLLMRELLGESLRELRTSENLTLREVSGRARVSLGY
                     LSEIERGQKEASSELLNAICGALDLPLSTLLRRVSDKIDAYAGVTQLPLRSPRVAAA"
     gene            47339..47533
                     /locus_tag="PKJ61_00530"
     CDS             47339..47533
                     /locus_tag="PKJ61_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3046 domain-containing protein"
                     /protein_id="HOC12350.1"
                     /translation="MRETALRARLEKHLGYPYVLAWSETVALAGVGHRTVTQALAAGV
                     PCGQIWRAAWEALELTEHDR"
     gene            47727..48770
                     /gene="recA"
                     /locus_tag="PKJ61_00535"
     CDS             47727..48770
                     /gene="recA"
                     /locus_tag="PKJ61_00535"
                     /inference="COORDINATES: protein motif:HMM:TIGR02012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOC12351.1"
                     /translation="MAVADREKALAAALAQIEKQHGKGSVMRLGDETRQPIEVIPTGS
                     IALDVALGIGGLPRGRVVEIYGPESSGKTTVALHVIANAQAAGGICAFIDAEHALDPD
                     YAAKLGVDTDALLVSQPDNGEQALEIADTLVRSGALALIVIDSVAALTPRAEIEGEMG
                     DSHVGLQARLMSQALRKMTGALSGAGTTAIFINQLREKIGVMFGSPETTTGGRALKFY
                     SSVRLDVRRIETLKDGSEMVGNRTRVKVVKNKVAPPFKQAEFDILYGQGISREGSLID
                     LGVDNGIIRKAGAWFTYDADQLGQGKENARNYLKANPEVAAEIEQKILSKLGIGANGV
                     PDGIDPVTGEVEF"
     gene            48773..49315
                     /locus_tag="PKJ61_00540"
     CDS             48773..49315
                     /locus_tag="PKJ61_00540"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HOC12352.1"
                     /translation="MTDPDGRFDEPDDEADPVGVAREIALRQLTVRARTRVELARALL
                     RRKVPTEVAEQVLDRLEEVGLVDDAVFARDWLAAGERRHKSRRALAVELGEKGVAREL
                     IDEAVAELDGEHDYLVARGYAEKKAAGLAKVPPDAQYRRLAAALARRGFNASVVAQVT
                     REVLAEVRTEDGLHNDWDGA"
     gene            49490..51016
                     /gene="rny"
                     /locus_tag="PKJ61_00545"
     CDS             49490..51016
                     /gene="rny"
                     /locus_tag="PKJ61_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020576496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HOC12353.1"
                     /translation="MELLLGIVLGVLVLLVVILAFVLVMLVKGQPAPRRGAPNKRGPR
                     QSARLRSPQPLVGTLVPTQVPAEAEAELRSRRQAADRREELLAQREDRLNRFLSELHT
                     RETHLDEIGAALDRREAALDDAESEQVEALAKVAGLSPVQARNDLLTQVEKSSRLHAA
                     QLAREIESEAKRNADAVARRIIVTSLQRLAAEQTTESVVAAVPIPGDDMKGRIIGREG
                     RNIRAFEQITGVNVLIDDTPESVLLSCFDPVRRETARLTLTELVADGRIHPARIEEVH
                     ERSQRRVEELCIRAAEDALIEMGITDLDAGLLPYLGALQYRTSYGQNVLRHLIECGHI
                     AALLAGELGLDVDTCRRAAFLHDIGKALTHEAEGSHAKVGADLARRHGEHPDIVHAIE
                     AHHNEVEPRTVEAVLTQAADAISGSRPGARRESLEIYVKRLQRLEDIAKEHEGVDKVF
                     AMQAGREIRVMVTPELVNDAQSLVIARDIAKRVEEELTYPGNIKVTVVRESRAVALAK
                     "
     gene            51145..52599
                     /gene="miaB"
                     /locus_tag="PKJ61_00550"
     CDS             51145..52599
                     /gene="miaB"
                     /locus_tag="PKJ61_00550"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017935826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HOC12354.1"
                     /translation="MRTFEVVTYGCQMNVHDSERIAGVLLDAGYQPAEAGSQADVVVF
                     NTCAVRENADNRLYGNLGHLAPVKQAHAGMQIAVGGCMAQKDRHTVVQRAPWVDVVFG
                     TNNLGSLPVLLERARIESESQVEILESLERFPSTLPTRRDSAYAAWVSISVGCNNTCT
                     FCIVPALRGKETDRRPGDVLAEIKALVAEGVQEITLLGQNVNSYGVEFGDRGAFAKLL
                     RACGEIDGLERVRFTSPHPAAFTDDVIAAMAETPNVMPSLHMPLQSGSDAVLKTMRRS
                     YRSTKFLGILDRVRAAIPHAAITTDIIVGFPGETDADFEATMEVVRASRFSAAFTFQY
                     SIRPGTPAATMPDQVPKAVVQERYERLVDLVGELAWAENSKLVGQRVEVLFADGEGRK
                     DAATHRMSGRARDNRLVHVAVPDDPALRPRPGDLAEVEISYAAPHHLNADGGLLSLRR
                     TRGGDAWAARQAPPEAEAAPGRTVGLGLPKLVRA"
     gene            complement(52691..52855)
                     /locus_tag="PKJ61_00555"
     CDS             complement(52691..52855)
                     /locus_tag="PKJ61_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HOC12355.1"
                     /translation="MDIVEQIKDAVADHAAEIKEGVEQVGDFIDEKTGGQYAAQVDQA
                     QEFVKDQLGN"
     gene            52959..53909
                     /gene="miaA"
                     /locus_tag="PKJ61_00560"
     CDS             52959..53909
                     /gene="miaA"
                     /locus_tag="PKJ61_00560"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOC12356.1"
                     /translation="MFHVAIPAVALNGPTASGKTAVAVEVARLLADAGLAAEVVNADS
                     MLVYRGMDIGTAKPTVAERGGVPHHLVDILDVTETASVADFQRLARAAIADCRQRGVV
                     PLLAGGSALYVHAILDAFEFPGTDPAIRSRLEAELDKLGSPALHARLTELNPEAAAGI
                     LPGNARRIVRALEVIELTGTFTSVLPEWTYALDGVLQFGLEVPREVLDERIAARVDQM
                     WADGLVAEVEKLVDRGLRSGRTASRALGYRQVLAFLDGELTESEAREATITGTRRFAR
                     KQLGWFRRDPRIVWLPAGPQSAITIASVVTDKVLGGPQEA"
     gene            53909..54718
                     /gene="dapF"
                     /locus_tag="PKJ61_00565"
     CDS             53909..54718
                     /gene="dapF"
                     /locus_tag="PKJ61_00565"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002527359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HOC12357.1"
                     /translation="MDIEFAKGHGTRNDFVLFADPDNLYDLTAEQVRYLCDRRAGIGG
                     DGVLRAVRTNQVSQWAGSRADWFMDYRNADGSIAEMCGNGLRVFLRFLAEEDLIDPSG
                     VTAVATRAGLRSGWLVDDGRIAVSMGAVSIGDQVTISHGGRTWPATAADVGNPHAVVE
                     VASAAELDGLDLSLAPTWSPADAFPNGVNTEFVLREGPGRLRLRVFERGVGETDSCGT
                     GVVASAAVLGGAQNCAVQVLGGRLEVRLGDPDAVLIGPAAVVARGRIDLPS"
     gene            54731..56119
                     /gene="hflX"
                     /locus_tag="PKJ61_00570"
     CDS             54731..56119
                     /gene="hflX"
                     /locus_tag="PKJ61_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007077541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOC12358.1"
                     /translation="MTEEESLIESSVDLQARHSLRRVAGMSTQLADITEVEYRELQLE
                     RVVLVSVWTSGTEADADYAMAELKLLAETAGSQVLEGLVQRRSHPDPATFIGRGKVDE
                     VREVVLATGADTVICDGELEPAQLRNLEDRVGVKVIDRTALILDIFATHAKSAEGKAQ
                     VELAQLQYLRQRLRGWGGNLSRQAGGRAAGGAGIGGRGPGETKIETDRRRIKTRISIL
                     RKRLRELDAVRDTKRSQRTRNQVPSVAIVGYTNAGKSSLLNRLTGAGVLVEDALFATL
                     DPTTRRTQTTDGRVYTLTDTVGFIRHLPHDLVEAFRSTLEESVNADLLLHVIDGSDPD
                     PFGQVTAVRTVLAEIGAANVAEQLVINKIDTAEPNELLALRGRFPDAVFVSARTGAGI
                     ADLLARVEERLPRPAIEVHALVPYDRGDLINKIHLTGEFVSSEHTENGTAVVARVNAD
                     LAGELARYAADG"
     gene            56112..58079
                     /locus_tag="PKJ61_00575"
     CDS             56112..58079
                     /locus_tag="PKJ61_00575"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002527357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HOC12359.1"
                     /translation="MAEAVPIGQLAEQILEAAIAGIGGAARPGQGRMATEVARTLDTG
                     EHLLVQAGTGTGKSLGYLAPALAALVQQREARVVVATATLALQAQLAVKDIPAAVAAC
                     AEVTGQQVSHAVLKGRSNYACLLRVRDGQGAEQDALLPEPGEATGVGAEVVELRRWAE
                     TEAEHGSLADRDDAPAHTPLAWAQVSVPVRECVGAQRCPYGTECFVEKSRERARASQL
                     VVTNHALLAVDALHGGTALPEYDGVIIDEAHELVSRVTGAASVELSPQQVERVAKRCL
                     AWVGDEVGLEFLDAADALQLGLDADAVGRVLDPSAPVLEALRVVRIAARHAVSALSGG
                     SDEEPERRQAQGATQEVFDIAERMAKLEEHDVVWISESDRFGRQVNCAPLSVAGLLRS
                     QVFDNHPSVLTSATLKVGGDFKAVAASLGLGGEGGSWRGEDVGSPFDYRSQGILYVAA
                     DLAPPGRDGISAETLAEIAELLWAAEGRTLGLFASQRAAEAAARYCREHVPDRLVLCQ
                     GDAHLARLTAEFISDPEASLFGTLSLWQGIDVPGDTCRLVIIDKIPFPRPDDPLMQAR
                     QQAVADAGGNGFMAVAATHAGLLLAQGSGRLIRTLTDRGVVAILDSRLVKARYGSFLR
                     ASMPDFWMTTDREIAIGSLRRLAEAVESPNQ"
BASE COUNT         9176 a        19202 c        20402 g         9299 t
ORIGIN      
        1 gcgcggcagg tggagcacga cgagcagcgc acgccgggtt cgtggtggaa ctggtcgtcg
       61 gtgaagtccg cgctggaatg gctgttctac atcggtgagc tctccagcgc cggtcgcaac
      121 acccagtttg agcggcgcta cgaccttccc gagcgggtgc tgccggcctc ggtgttggcc
      181 acgtccacgc cgagcgagga agaagcccgg ctcatcctgg ctcggcgcgc ggcggccgca
      241 cttggcgtct tcacccagaa gtggctggcc gagtacttct acaccgatcg agtcggcacc
      301 atcgcggcgc tcgagtcgct gctcgagtca ggcgaggtcg agccggtgga cgtgtgtggc
      361 tggtcggcgc cggcctacct gtggacgcag gccgcgcgtc cgcgcaagct cagcgtggac
      421 gcgttggtgg cacccttcga cacgctggtg ttcgaccggg atcggctgct ggacaccttc
      481 gacgtgcact accggatcgg gctgtacacc ccgaaggccg accgggtgca cggctattac
      541 gtctacctgt tcgtgatgga cgacgtcatc gccgcccgcg tcgacctcaa ggcagaccgc
      601 aaagccggac gcctgctggt gcagtcggcc tggttggagg acggcgccgc tgcggccgag
      661 acgggcgcga ggctcgccgc caagctgcgc gagatggctg actggctcgg tctcaccgat
      721 gtggtggtcg gtgagaccgg aacgctggcg ccaaccctga agaccgctgg ccgagcctga
      781 cgaagcccga tggctcagtg gctgttgggc cggtggctcg gcggacggtg actcgccggc
      841 aggcgcttcc cacgaccttc gggatcgccg ttgtcctcgg cgtgccgctt ggcgtaggcc
      901 gcatcctcgg caccagcgcg cacgtgggtc gggtcgagca cccgggccag gaaggtccgc
      961 gtccgctctt ccttggggtt gccgatgacc tcctcgggcg gaccctcctc gacaaccacg
     1021 ccgccgtcca tgaacagcac ccgatcggcc acttcgcgag cgaacgccat ctcgtgggtg
     1081 accaccatca tggtcatgcc accagcagcg aggtcgcgca tgaccgacag cacatcgccg
     1141 accaactccg ggtcgagggc ggaggtgggc tcgtcgaaga gcatcatctc cgggtccatg
     1201 ctcagggccc gggcgatcgc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 atgcagacca cttctcctcg atggatggtg gtggtgatgc ccttgagcac ctcgaggtca
     1381 ccgaaggact tgtgcaggtc gtagacctgg atctcgtcgt tgcgatctcc ggtgggtaca
     1441 accgctgggg tgagggtggt catcgggtgg ccttccgctg ctgggcttcc atgcgccgca
     1501 ccaggtaggt caacggcaag gtgatgatca gatagcacag gccggccacg atgagcggcg
     1561 tgaggttgcc gttgctgttg gcgaggtcgc gtccgtactt ggtcaactcg aagttggaga
     1621 cagtgagccc gagcacgtag acgagcgaag agtccttggt gagcagcacc agttcgttgg
     1681 tcagtggggg tgtgatgatc cggaaagcct gcggcaggat gattcgctgc atcgccattc
     1741 cggcgggcat gcccaaggat cgcgcagcct ccatctggcc cttgggaacc gcttggatgc
     1801 cggcccgaat ggtctccgac atgtaggcgg ccgagaccat gccgagcgcg gtccagaccg
     1861 tgccataagg ctcccagggc aggtgcaggc cggggaaagc gatcggcagc aggttgaagg
     1921 cgagcaggac gatgatcgcc ggaaccccgc ggaagaactc ggtgtaggcc gtggccagcc
     1981 aacggtaggg gccgatctgg gacagcttca tcagcgccag gatcgtgccg aggaggagac
     2041 cgaagacgaa ggcgccaatg gtgtacagga tcgtgttgac caatgcccac agcaggttgg
     2101 ggccaagggt ctttgcgatc atgtccggcc ggaagaagac ctgctgaagc tgccggccat
     2161 cggcgagcag ggcgaccgca aggagcacca aggcgaggat cacgtattgc gcaattcgga
     2221 agcgctgcgc ccgcttgcgc ggtgagagct tctttcgcgg gatggtggtt ggagcgacgg
     2281 tcgtactcat gcggcctcct caggtttggc cttggctgga cgccgtccgt ccgaccgggg
     2341 cgtttcggca cccggccgga cggacaacgg aagtggggtc actcggtcgg cgcgtcgact
     2401 ccaaaccact tcttgtagat ggtgttgtag gtgccgtcgg ccttggccgc cttgagcgca
     2461 tcgttgatcg cggtgagcat ggcgggattg tccttggcgg tgttgaaacc gtagtgctca
     2521 ccggtctcga actccttcac cacggtggtg gtggggttgt ccttcgcgaa gtcgaagacc
     2581 acgccattgt cgttgatatt ggctgcggca ttaccggcca gcacggcgtt ggcggcggtg
     2641 ggcatgtcgt cgtagacctt cacctcgtag ccgtactcgg ccttgtggtc ctcggcgtac
     2701 ttctggccgg tggtatcggt ctggacggcg aggatcttgc ccttcaggtc ctcaaggccc
     2761 atgatgccgg aatcggtctt gaccaggagt gcctgggtgg cggcgaagta gggatccgag
     2821 aacggcgcgg ccgcattgcg ctcgtcggta atcgtgatcg ccgcagcacc ggcatcgcac
     2881 ttcttggccg taaacacggc tcccgaagtg atctggccga agtcgatgtc gacgatgtcc
     2941 tgggtcaccc cgagcttctt ggcgacgagg tccatgagat cgacgtcaaa gcccacgatc
     3001 ttgcctgcgt cgtccttgaa ctggaacggc ttgtaggcca gatgggtgca gacggtgagc
     3061 tttccagcgc tggctaaagc cggggcggcg gccgaaccac cggtcggggc cgatgtggcg
     3121 ggctgggagt agttggcaca gccggtgagc gccagggtca gggcggccgc gccagcggcg
     3181 aggcccatga gtcgcttcat ctaggtcgtt cctttcaacg gtgactgggc gaatcctgcc
     3241 actgctttgt ttcagtttga agaaggatcc gcggttcaga acaacccgac tgccgccgat
     3301 gaccgtggaa ttggcgtcta gcaatagaat gggcgggtgc gcccgtcggg tcgcgccagg
     3361 tgaaacgaac gaggaaagca cggtcggacg tgggtctact ggacaagatg ttgcacatgg
     3421 gcgaaggccg ccagctcaag aagctgaagg tggtcgccga ccaggtcaac tcgatcgaag
     3481 ccgacttcga agccatgtcg gacgaagagc tccgcggcca gaccgccgac ttcaggcagc
     3541 ggctggagaa cggcgagtcc atcgattcga tcatgccgga ggccttcgcc accgtccggg
     3601 aggcgaccaa gcgggtgctc ggcaagcggc atttcgacgt ccagcttcag ggcggcgccg
     3661 cgctgcactg gtgcaacatc gccgagatga agaccggtga aggcaagacc ctggtcggcg
     3721 tgctgccgtc ctacctgaac gctttgagcg gcaagggcgt ccatgtggtc acggtgaacg
     3781 actacctggc gaagtttcag tccgagcaga tgggccgcat ccatcacttc ctcggcctga
     3841 aggtcggcgc gatcctgtcc cagatgaccc ccgccgagcg tcgcgaggcc tacgcctgcg
     3901 acatcaccta cggcaccaac aacgagttcg gcttcgacta cctgcgcgac aacatggccc
     3961 tcagcctcga cgaatgcgtc cagcgcggcc accactacgc catcgtcgac gaggtcgact
     4021 ccatcctggt cgacgaggcc cggaccccgc tgatcatctc cggtccggcc gaggacaact
     4081 cgtcctggta ccccgagttc gccaagctcg tccgctcgct gcatcgcgac gagcactacg
     4141 aggtggacga gaagaagcgg acggtctccg tcctcgagcc cggcatcacc gcggtcgagg
     4201 accggctcgg catcgagaac ctttacgagt cggtgaacac gccgctgatc tcctacctga
     4261 acaacgcgat caaggccaag gaactgttca agcgcgacaa ggactacgtg atcatggaag
     4321 gcgaggtgct gatcgtcgat gagcacaccg gtcgcaccct gatcggtcgc cgctacaacg
     4381 agggtctgca ccaggcgttg gaggccaagg aaggcgtcga gatcaaggac gagtaccaga
     4441 cgctcgccac gatcacgctg cagaactact tccgcatgta caccaagctc gccggcatga
     4501 ccggtacggc caagaccgag gaatcggagt tccagaagat ctacggcctg ggtgtgctgc
     4561 cgatcgagac caacaagccg atgattcgcg tcgatcaggc cgacctgatc tatcgcaccg
     4621 aggaggccaa gttcgccgcc atcgtcacgg acgtggtgcc ccggcatgaa gccggccagc
     4681 cgatcctgct gggcaccgcg agcgtggcca agtccgaggt gctctcccgg ctgctgaagc
     4741 aggctggggt gaagcacgag gtgctgaacg ccaagaacca cgaacgtgag gccgcgatcg
     4801 tggccatggc cggacgcaag ggcgcggtca ccgtcgccac caacatggcc ggtcgtggta
     4861 ccgacatcat tctcggcggc aaccccgagt tcctggccga tgcggtgctg cagggcaagg
     4921 gcctcgaccc ggtcgagcac gccgacgagt acgaggccgc ctggcccgag accctgaagg
     4981 cgatcgagca gcaggtggcc gacgagcacg acgaggtcgt ggccctgggc ggcctgtatg
     5041 tggtcggctc ggagcgccac gaatcacggc gcatcgacaa ccagctgcgt ggtcgatccg
     5101 ggcgtcaggg cgacccgggc gagagccggt tctacctgtc gctgggcgac gacctgatgc
     5161 ggttgttcaa gtccgacatc atcgactggg tgctgcagac cctgaaggtg cccgacgacg
     5221 tgccgatcga gaacgcccgg gtgagcaagt cggtgcagca ggcccaggag caggtcgagg
     5281 cgcagaactt cgagatgcgt aagaacgtac tgaagtacga cgacgtgatg aaccgccagc
     5341 ggcacgccat ctacggcgac cgtcgccgcg tcctcgaagg tgcggacgtc gagcagcagc
     5401 tgcgcggcac ggtggacacc gtggtcgagc agtacgtccg ctcgctgacc gagggcttcg
     5461 tcgaggactg ggacttcgac gcgctctgga cgcagctgca caccctctac ccggtcacgc
     5521 tgagtcaggc cgattacgag ggtcgcaccg acctgaccgt ggacgaactg gtcgccgact
     5581 tcaaggctga cgcgcaagcc gcctacgacg cccgtgaggc cgccctcggc gaagacacca
     5641 tgcgcgagtt cgagcggcag gtgctgctca ccgtcctcga ccgcaagtgg cgcgagcacc
     5701 tctacgagat ggactacctc cgcgaaggca tcggcctgcg tgcgatggcg cagcgcgacc
     5761 cgctggtcga gtaccagcgc gagggcggcg acatgttcaa cacgatgatg gaagccttca
     5821 tggaggaggt cgtgggctac ctctacaacc tccaggtgca ggtcgagccg gctccgcagg
     5881 tgggtctggt ggctggcgcg gacggcgtcc cgatcaccgc ggaggacctg gtgccggcgg
     5941 cgagcgcgcc ggagcggaag tcgtccatgc cggcggagtc gagcctgacc aagcgtccgg
     6001 cgctgaaggc caaggggctg gaatccaagg cgccgcgggc aatggcctac tcggcgccga
     6061 ccgagaccgg tgaggtggcc aagaccgggt cggcgatcac ggatgacccg tacgccgggg
     6121 ttggccggaa cgctccctgc ccgtgtggct cgggcaagaa gttcaaggtc tgccacggcc
     6181 gcacggcctg agccttacgc ctgttccacc gtcatcccgg cgctgagccc tgcgcctgtt
     6241 ccaccgtcat cccggcggag gccgggatct cagcacgatc ggtggagctg agcccttccc
     6301 ggcctaggct ggagttggct ctgagcaggg aggctcgcga tgcgtgtcgg catgtcttcg
     6361 gcgaggattt cggtggggct ggcggtcgcg ctggccgtgc ttgggtgcac cggaccggtc
     6421 gaactagtgg tggctcagga agcggccctg ccccaggcgc cgacgacgac ggcagcggcc
     6481 ttccccgact tcgatggcga cggcacggct gatctcgtct acggcgtggg cacgaccagg
     6541 agctacgtgg ccctgtctta cggctccgga cgctcccaga ccatcacccg cacggacgcg
     6601 ggtggcaccg atcccgcgtc cgacgacaac accaccggtt tcggccaggg cctgttggcc
     6661 cgcaacctga acggtgacgc ctacgccgac ctggtcgtgg tcgacgcctc gcaaggggcg
     6721 ggttctgcgc tctacctggt cttcggttcg gcggacgggc tgcggcccgc agcggccagg
     6781 cgttacccgg tgccgtcggg gatgtacggc ttccacggct ctccggctct gctggagtct
     6841 ccgggccggc tgctggtggt gtcgacaggc gtgccaggga tcaagggcgg agcggtgctc
     6901 gcctaccagc tgggtgcgga cggtctgccc actggcgctc cgaagctgct gagccagcag
     6961 aaccttcccg gcaccgacga gaacggcgac gccttcggca cgtcgatcgc tgcgtccggg
     7021 aatctgctgc tgatcggcat tcccggtgag gacctggaca gcgtgcagcg cgccggcgcg
     7081 gtgctggtgc tgaagtacgg cggcgggttc aagttcaccg gcacaaccat cacgcagagg
     7141 accgcgggcg tcaccggaaa ggccgagcgt tgggacggct tcggccgctc ggtcgcgatc
     7201 gccggtggtt atgctgcggt cggtgtcccg ggtgaggatc tgggcaagcg ggacgcgggt
     7261 gcggtgcacg tgttcaaagt gaccaaaggc gagctgaagc acgtcgctcg ggtcagtcag
     7321 gacagcaaat gggtgtcggg caagggcgag accggcgaca tgttcggcta ttcggtggcg
     7381 atcggccgca cctgcgatgg gtaccccggg ctgctggtgg gggcgccgca cgaggcgatc
     7441 ggaagcatcg cggcggctgg ttcggcgtgg ttgatcccga tcgagcggca tcccggagac
     7501 tgcccggtgc agctgtacga gggcggtcgc cttggtggta ccgcccgcga gatggcgagc
     7561 gtcggcgccg cggtgtcgat gttgcgcagt ggcaaggtcg gtgcggatac ggtcgtgctg
     7621 acttcccccg gcatgtctga agagggcgtg tacggccggg tgatcactca ggccacaccg
     7681 ttctctggca atgcggcgac ggtcgccaag gacctgatcc tgaacgagaa cagcaccctg
     7741 gtactcagcc cgccagcggg ctgatccagc cggctccgtg cacgacgttc ggacgcaggg
     7801 cgatcgatag gtgtgtgccg acccactggt cccgccgcaa cgcgacgcgt agcgcggcgg
     7861 ctcgggaggc tgagccttgg cgcaggtggg ccgatacctc cagcacccgg gcgttgggcg
     7921 cctggacgcg gatcgactgc aggcggagcc cgctgcccag ccgggcaccc cgaaggtgtt
     7981 cgatcagcgc gagtagctcc ggatcgagcc agcgttcgag ttggagtggg gagcgctgcc
     8041 cggacagcac ttcgacgacg gctgccatca gcgcggacgc gaactgccgc agctgtggtt
     8101 ccacgggcga atctgcctgg acgacctcgg tggcgtcgag ccagggcagc gtgggctggg
     8161 tgtcgccgag gtattcggcg ggcaccagca gcgggatcgc cggcggacgc gagatgggcg
     8221 cggcgtggca tttcgcttgg ctgggatgca tcggcagccc ctctcgattc gacgctgcca
     8281 ccacgatcgc ggttggcgtc cgccgaattc cagggtgcac gaggcaatct gtggataact
     8341 cctggtcatc cacaacccgg atcagggtga gaacagcagc accaggccac cgcaggtgta
     8401 gccgatcatc accagcagca tcgcgagctg gccggacgtc cgacgcttgg gcggcagcag
     8461 ggtcaggctc acctcatgcg ccaccaggac gccgatcacg tggccgccga cgatggcgat
     8521 cagttggatc agcgccgtga tcgccggggt ttccaaccag aacaggttga tgcccagctc
     8581 ggctgtgccg aaggtgttcc agcccaggcc gagcggatcg gagaggttga tcagcgtccg
     8641 ctgcccttcc agcacgagca ggctcaggta gtgcgcgacg gcatagccga tcacgatcgg
     8701 gatcacgctg gcggcgatgc ggtcgaaggt gcgtccggtc agccgaccca ggccgcgtgc
     8761 gcccagggca agggtgacgc cgaccagggc gaccatgccg agcaggccga gcgtccccca
     8821 gaaggtgccg ggcaacgccg aggtctgcgt ggtgcgcacc caccaggtgg tgccggtgaa
     8881 gccgtcgaac gcggtgatgc cgagcagtgc cgtgaccacc gcccaggtgc cgtgcggagc
     8941 cgtggaggtg ctgaggttgc gcagcgggtt caccagcaag ggccgctgcc accacgacgc
     9001 cctggccacg gtcgttgcgt acacctcgaa cgggtcggac gcggccagcc accgttcacc
     9061 gaacaccacg gccccgccga ggcagccaat cacccaggcc agccaccacc cctgcagtac
     9121 cgcgagggtg ttgttgtccg gctggacgag ttccagccag ccgaagccga tcagggccag
     9181 cgccgccgga tactggccca gccgctgcca cagcgaagcg ctgagcggcc ggaaaccgtc
     9241 ggccggcaag ccaagatgca ccatgccgcg gtgcagggtg cgcaacgggt tcagcgtccg
     9301 ccagaccggt ccgagcagca gtgaaagcgg caccagtccc acccaaaccc agacgtaggt
     9361 gaagccgaag accgggttgg ttacccggtc ctggccgaac cagagcgcca tccccaccca
     9421 ggcgaacgcg aacaggccga gggcggcgac caggccccgc gtccacttgg aatcgatgaa
     9481 ccgggagagc cggggaagcg cgcgtccgcc ggggtgcccc gaccaccggc tcgtccgcca
     9541 agcgaaggcc agcaccccga aggacaccag caggacgacc accgcgcccc cgacgacggc
     9601 agggaacggc agcggcaggt cctgccgcga gccgaggccg tgcaggggga gcagcagggt
     9661 cactggatct gtagttgcag gagtgtgaag accggctcgt gcgactccac ctcgaagcgg
     9721 ccgaccttgt ccatgaccag atccctggtc accgtctccc cggccttgac ctcgatctcg
     9781 acgtcgaaac catggacgtg caccgcatcg gcgcgatcag aggtgatcgt gagcaccaga
     9841 tgctgcccct tcaccagttc gaccttttcg gcgatcggct cagccttgcc gtccgcgagc
     9901 gtgatggtca cctgcttggg tggcggtggg gcgctggtcg aggacgcgcc ggcggcacag
     9961 ccgctgagca gcacgagcgc cagccaggtc acaagcagtc gtttcattcg tcctcctgcg
    10021 ccgattctgt caccaacttc gcaggcttct gctgagtagg gtggaagcgt gagtgatccg
    10081 gtgtcggtga ccgaggcggc gctactggcg cgcgaccagg agtcccagcc atggcagcag
    10141 gctttcgtgg cgatgttgag cgggccgctg acccgggaac agctgatcga tcgggtcacc
    10201 gagcgcatcg gttacgcgcc gcggttccgc aggctcgtta ccggctggcc gctgcccacc
    10261 tgggttgacg acgtcggctt cagcgtgggc ggccacgtcc gggaggccac cctcgccccc
    10321 ggggagcgac ttgagagctg gctcagcgcc aagttgcagt cgggcctcga ccgtgcccat
    10381 tcgccctggg aggccaccct ggtgcacggc atcgcgcccg gcaccacagc cctggtggtg
    10441 cggacgcacg ccgccttggt cgacgggtac gacaacgtcc atctgctgca ggagctgttc
    10501 gacgagtatg ccgaagacat cactgacgtg gatgatgcct ggcagccgcg tgcggccgag
    10561 gcgttcgggg tcgcggatgt gctgagtggc ctgaacgacc cgctgcaggc gctgaagagt
    10621 gcggccaccg ggctggtcgg catggtcgag aacgccgtcc ggacggcttc ggcgaccgcc
    10681 cgtccacagc atttggccgg tgtcgaggtc gatctggacg cgctggccgc cgtccgtgaa
    10741 cgcttcggtt gcacaaccca tgacgtgatc ctcgcgctgg ctacggccgg cgtccgcggt
    10801 tggatggtcg agcacaaccg ggaactggtc gaccaggtcg cgctggttcc gctggctgtg
    10861 gccgagcccg cggtgctgga gagtgcgatc ggctgccggg tggcaccgtc ctgggtcgcg
    10921 ctgccgatcg gagtggcgga cgcggccgac cggctgcgca cgctggccac cctcacccgc
    10981 gcgcgcatcg acaccggcgt gagcgtgccc gcccgtgacc tgatcgacct cgccgggttc
    11041 gcgccaccca ccctgcacag cgtcgcctcg ggaacggtgg ccgcggggcg gccacactcg
    11101 gtgatggtga cgaacgttcc cgggccggcg accaggcggt atctcggccg ggcgaggttg
    11161 agccgggtct atgcggtctc ggcgaccacg gactcccagg acgtgtcggt ttcgatcgtc
    11221 agctacgccg gacgggtctc gttcggcgcg gtcgcggtcg agccgctcgg ccactgggct
    11281 gccgacatcg acgcggaact gaccgcgctg cgaggggagg cgagatgacg ctggtcttcg
    11341 tgccggtgac ggttgcggag ctcactgatt gggccaccac cggatcactg ccaggccgac
    11401 gggcggcttt cgcaaccacc cccggactgg tcgcagcctt cgaggtcagc gaccccgaag
    11461 aagccgaaca catcgcactc ctagcggcga gtgtggccgg gttggcggcg tccggtactc
    11521 gactggtcgc ggtagcggag gcgtccacta ccgccggggc tgacagcggg gcggacttcg
    11581 gcgaagtctg tgtgaccgac ctgccgtacc gcacggtgca gagcctgttc gccgacgaag
    11641 ccgcggccga gggcctatcg gaagccgcgg cggccgtccg cggagctgac ctggagaccg
    11701 cgtgggacca ccccgcggtg gttaaagtgc tccggactgc tgagttgctc tggcatggcg
    11761 ccggggaatg gtccagccta agtactggct gaaagaactg gttgagccac gaggcagagg
    11821 gaggcagcat gcccaggtcg atctggaagg gcgcggtgtc cttcgggctg gtgaccatcc
    11881 cggtgaagct ctacggagcg acccaggaga aggacatcag cttccggcag gtgcatgccg
    11941 ccgacaacgg acgcattcgg tacaagcggg tctgcgaggt ctgtggcgag gaggtgccgt
    12001 acgcggagat cgccaagggc tacgaagccg cggacggacg gatggccgtg ctcaccgatg
    12061 aggacttcgc cgacctgccg gcagcgcagg gcaaggcggt ggaggtggtg cagttcgtcg
    12121 acgtggacga gatcgacccg acctacttcg accggacgta cttcctggag ccggagaagt
    12181 cgggcaacaa gccctacatc ctgctacggc aggcgctggc cgagagcggc aaggccgcgg
    12241 tggtgaaggt ggcgttgcgg tcgcgggagt cgttggcgct gatccgtccg gtgggcgagg
    12301 tgctgcggct gcacaccatg atctggccgg acgaacttcg tgacgacgac ttcgccgcac
    12361 ccaccgaaga ggtgaaggtg aacgacgccg aggtcggcat ggcccggatg ttcatcgagc
    12421 agttgagcgg gaagtgggat cccgaccagt tcaccgacga ctaccgcgaa gccctggaga
    12481 aggtcatcga agccaagctg gagggcgtgg aactgccggt cgaggctgag ggcactccca
    12541 gtggcgccca ggtggtcgat ctggtcgagg cgctacgggc ctcggtcgaa gcggccaagg
    12601 cgcggcgggc gcaggcccag gccagctagc tccacaacgg ttccccgagc ttgtcgaggg
    12661 ggctatagag tcggctggtg gacgccatca cctttgtacc caagcccgtc aacgagccag
    12721 ttctttgcta cgcaccgggc tcgcccgagc gcgccggctt gaagcagacc ctcgccacga
    12781 tcgcgaccga gccggtcgac gtctttccgg tgatcggtgg cggctggacg ccgcgcgctg
    12841 acgcagccac gctcgaggtg cgcgcgccgt ccgatcattc gaccttgttg gcgcgggtgc
    12901 gccaggcctc ggccgcggac gcgtcggcag ccatcgctgc cgccatggcc gcgcggacgg
    12961 cctggtcggc gacttccttc gacgagcgtg ccgcggtctt cctgcgggcc gccgagctgc
    13021 tggccggacc tcatcgcgac ctgctgaacg cggccacgat gctcggccag ggcaagaccg
    13081 cccagcaggc cgagatcgat tcggcctgtg agctgatcga cttcctgcgg ttcaacgtgg
    13141 cgttcgcccg cgagctgtac accaaccagc cgatcagctc gccgggcgtc tggaaccgca
    13201 tggactaccg gccgctcgac ggcttcgtct acgccatcac cccgttcaac ttcaccgcga
    13261 tcgccggcaa cctgcccacc gcgccggcgc tgatgggcaa cacggtgatc tggaagccag
    13321 cgctcaccca gaccttcgcc gcgcagttct ggatgcagct gctcaccgag gcggggctgc
    13381 ccgaaggcgt gatcaacatg ctgcccggcc acggggccga ggtctccgat gccgccttga
    13441 accaccacga tctggcgggc atccacttca ccggctcgac ccgggtcttc cagggcctgt
    13501 ggacgcaggt cggcgcgaac atcgccaact acggcgccta cccgcgcctg gtcggggaga
    13561 ccggtggcaa ggacttcgtg gtcgcccacc cgtcggccga tccggctgcg gtgatcgcag
    13621 cgctggtgcg cggtgccttc gagtactccg ggcagaagtg ctcggcagcc tcccgcgcct
    13681 acctgccgca gagcctttgg gacacgatca aggatgagtt gatcgcccag gtggaggcgc
    13741 tgacggtggg cgacgtccgc gacttcgcga acttcacctc cgcggtgatc gacgaacggg
    13801 ccttcgacaa gctgtccggt gcgatcgagc gggcccgcag ctcggcggac gctgagatcg
    13861 tcgccggtgg cacctacgac cgcagcgagg gctggtacgt gcgacccacc ctgatcacgt
    13921 ccgacaaccc gcaccaggac attttcacca ccgagtactt cggcccgatc ctcggcatcc
    13981 acgtctaccc ggacgcgaag tacgatgaag tacttcagct ggtcgacgac acgtccacct
    14041 atgccctcac cggcgcgatc ttcgccaccg atcggacggc ggtggcagcg gcgtcgacag
    14101 ccctgcgaca cgccgccggc aacttctacg taaacgacaa gccgaccggc gcggtcgtcg
    14161 gtcagcagcc cttcggtggc tcgcgcgcgt ccgggacgaa cgacaaggcg ggctcgctgt
    14221 ggaacctgat gcgctgggtc agcccgcgtt cgatcaagga gaacctgctg tcgccgacca
    14281 cgatcagcta cccgcacatg ctccccgagt gagccggaag tgaaccagct gcttgatccg
    14341 gagatcatcg aggccctgcg cagcgaggtg cacgatgcgc tgccgcgggc ggtggcggag
    14401 ttgaccgacc tggtcgcgat cccgtccatc tccttccccg cgttcgacca gtcgcaactc
    14461 gtccgcagcg cggaagcgat ccgggctctg gctactgcca ccggggtgtt cgacgaggtc
    14521 gcggtgcata tcgcggcgat cccaggaacg tccaagcatg gccagccggc catcctggct
    14581 cggcgggcac ccgctcccgg gcgcaagcat gtgctgctct acgcccacca cgacgtgcag
    14641 ccgccgggtg cggcgacgct gtgggactcg ccgccgttcg ttgctaccga acggaacggt
    14701 cgcctctacg gccgtggcgc cgccgacgac aagggcggcg tgatcgccca tctggctgcg
    14761 ttgcgcgcgt tgcggcaggt gcttggcgat gacctcggcg ggctcggggt gacgctgttc
    14821 atcgagggcg aggaggagta cggctcgcag tcgttcgggc agttcctctc cgaccaccgg
    14881 gacgcgatgg ccgccgacct gatcgtggtg gccgactccg acaactggaa cgcgcagacc
    14941 ccgggcatca cggtctcgct tcgcgggaac gtccgattca ccttgaaggt gcggacgctg
    15001 gcgcacgcgc tgcactcggg catgttcggt ggggcggtgc cggacgcgat gctggccacg
    15061 atccggctgc tcgacagcct gtgggacgcc gatggcgcgg tctgcgtcga gggtctggcc
    15121 acccgcgacg cggcgacgcc ggcccacacc gaagcagcat tgcgggaatc ggccgaactg
    15181 ctgccccaaa cccagccgat cggcagggga gagctgctgg cccgtatctg gaaccagccg
    15241 gcgatcaccg taaccggtat cgactacgca accgtcgacg aagcgtccaa caccctgcac
    15301 ccggaggtct cggtggtact gagcgcgcga gtcgcgcccg gtcaggaccc gcacgaggcg
    15361 tatgcggcga tccaggcgca cctgctggcc agggcgccgt tcggtgcgca actggagttc
    15421 agcgacctcg acgccggcct gggcttcctg gctgccgaag gctgggcaac cgaactcggc
    15481 cgggcagcgc tggccgcagc cttcggcgcc gagccggtcg acatcggtgt gggcggctcg
    15541 atcccgttca tctccgaact tgccgccgaa ttccccgaag ccgcgattct ggtcactggt
    15601 gtgcttgacc cgaccgcgag cccgcacagc ccgaacgaat ccctgcatct gggcactttc
    15661 gaacacgcga tcctcgccga ggcgctgctg ctggccgggg tcgcgaaggc ctgagccttg
    15721 tgccaccgtc atccaggcgt ttgccgggat ctctccgagc tagtggtgaa tctgggctag
    15781 atgagggcca attccggcac cttgacaaaa tagaacaagc gttcgacgat ggctgaatga
    15841 cgctggccat tgacccgact cgcgaggcgc tcctgcagcg tctgcgggcg caggcggagg
    15901 cgccggcgcg cagccgggcg gagcgttgcc ttccggtgcc ggacgctttc gccaaactcc
    15961 tgccggacgc tgggctcaaa cagggggcca cctactgcct cggggtggcc ggatcgctgc
    16021 tgaccgcatt gctgtccgag ccttcgcagg cgggttcatg gtgcggtgtg gtcggtatgc
    16081 ccgagttcgg ggtggaggct gcccaggccg cggggatcgc gctcgaccgg ctggtgctgg
    16141 tgccggatcc gggcgatcag tggctggccg tgaccgcggc tctggccgag gtgctgccgg
    16201 tgatcgccgt ccggcccggt ggccgcgtcc gcgaaggcga cgccgcccgg ctgaccgccc
    16261 ggctgcgtga ccggggcggc gtcctgctgg tgcacggcga ctggccgcgg gccgaggctc
    16321 ggctgcagct ggcggagccg cgctgggccg ggttggggat tggccacggc tgcctcacca
    16381 gccgggaggt ggtggccacc gccacgtcca aacggttcgc aggggtgcgc agcgtgcggc
    16441 tccggttacc ggatccccag ggtcggctgg cccttgcggc gccagtcgcc cccgttccgg
    16501 tcaggctgaa agcggccggc tgatgactcc caggtcagct gatcgacctc ggccgggcca
    16561 gtcccagccg atcctgagcg gtgcgcagcc ggcccgttgc ctggtgctct ggttcccggc
    16621 ctggtcggtg cgtgcgtggt cactggcaga ggggcagccg atcgatgtgc cgctggccac
    16681 ggtcgcggcg aaccggatca ccgcttgttc ggctgcggcg gtggccgaag gcgtagtgat
    16741 cggacaacgt cgccgcgagg cccaatcgcg ttgcccgacg ctgcaggtga tcgcgtccga
    16801 cgaggccagg gacgtccgcg agttcgagcc ggtggtggca tgcatcgagg agctttcgcc
    16861 gggggtgcag gtgctgcgtc cggggcagtg cgtcgtccgc gcccgcgggc tggccaacta
    16921 cctgggcagc gaggccgagg ccgccgagac cctgctggaa gccgtggtga ccaggctcgg
    16981 catcccggac ggacgggtcg gcatagccga cggcaggttc gccgccgccc aggccgctcg
    17041 cgccggcaat ccggtgcgga tcgtcccagc cggagaatcg gccggcttcc tggcgccgct
    17101 gccgatcagc cggctcgccg atgagggcct gtccaccctg ttgccgcgac tcggcgtcca
    17161 taccctgggc gacttcgccg cactgtccgc ggattcggtg cgcgaccgct tcggcaccca
    17221 ggggctgcgg ctgcacacct tcgcgtccgg cctcgatccc agcaccgtcc agccccgcac
    17281 gccacccccg gaactaaccc ggcggctcca cttcgagccg gccctgaacc tggtcgaaca
    17341 ggtcgcgttc agcgctcggg ccaccgccga ggatttcatc gccgggctga acgcggtcgg
    17401 gctggtgtgt accgagctcc gggtggagct ggtcggcgag cgggatgaac tggtggctcg
    17461 gaactggctg gccgccgggg tgttcgacgc cggcgcggtg atcgaccggg tgcgttggca
    17521 gttgcaggcc gcagccggac gccagctcgg cagtggcgtg gtgaacctgt tgctcgagcc
    17581 gatcgtggtg gatgcgcagg gcttccacat gcccgggctg ttcgggtccg ggccggacga
    17641 gcgggtgcac cacgtgcttt ccagggtgca gggcatgttg gggcacaccg gagtgctcac
    17701 cgcagaggtc ggcggtggcc gctggttggc cgaacgccag gtgctggtgc cctggagcga
    17761 ccggccgcag tcggaagcgc ccaggcagcg gccctggccg ggtcgggtgc ccgacccgct
    17821 accggccacg gtcttcagcg agacccgtcc ggtccggcta ctgggcggca gcggacgccc
    17881 ggtcgcactg aacagcaggg gagcggtgac cgacccgccg gtcgcgcttg aggtcgaggg
    17941 cggccgccgg acggtgatcg gctgggccgg tccctggccc atcgacgagc ggccctggga
    18001 cgccgaccgg catcgggtgg ccaaccggtt ccaggtggtc gacggctccg ggacggcctg
    18061 gttgttgctg ctggacgagc gtggctggtg ggctgaggcc aggtacgact gatggggttc
    18121 aacaacccgc cgatgacctg gcgaacgctg gaacggacgc tcagtgcccc gggtaggacc
    18181 gatgccggac aggtcgaggt gccgacttcg cacaagcgtg gccattatgt gccgccgccg
    18241 atcgtccgcc ccgacggcgg cgtgccgtat gccgaactgc acgcccactc ttcgttctcc
    18301 ttcctcgacg gggcgtctgg gccggcgcag ttggccgagg aggccgaacg gttggggctg
    18361 cacgcgcttg cggtcaccga ccacgacggg ttctacggca tcgtccgctt cgccgaggct
    18421 gcggagagca tgcagctgaa gacggtgttc ggggccgaac tctcattgga gcaatcggcg
    18481 gcgcgcaccg ggcaggccga tccggagagc acccacctgc tggtgctggc cgccggcgag
    18541 gagggctacc accggctggc gtccgcgatc accgcagcgc aactggccgg cggggagaaa
    18601 ggccggccgt actaccagtt ggacgagttg gccgaacggg ccgagggcca ctgggcggtg
    18661 ctgaccggct gccgcaaggg tgtcgtccgc caggcgctgg tcgacgacgg cccggctgcc
    18721 gccgccgcgg aactggacga actggtgcac ctgttcggcg cagagcaggt gttcgtcgag
    18781 ttgaccgacc acggccgtcc gctggattcg acgcacaacg acgtactcgc cgcgctggcc
    18841 gccgatcggg ggctgccgac gatcgccacc ggcaacgtcc acttcgccac gccggcgcag
    18901 caccggctgg cccaggcggt ggcagcggtg cgggccaatc gcagcctgga cgaactcgac
    18961 ggctggctgc cggccgccgg aacggcgttc ctgcgctcgg gcgacgagat ggccgcccgg
    19021 ttcgtccgct atcccggcgc ggtcgcgcgc accgtcagtc tggccgacgc gctggccttc
    19081 ccgctgcgcc gggccaagcc ggcactgccc aagcaggacg tgccggaagg acacacgccg
    19141 atgagctggc tgcggcacct ggtctggacg gcggtgccgg tgcgctaccc gaagctgacc
    19201 gagccgcagc gggagcggat cgaacgggaa ctggctgtga tcgaggccaa ggactttccc
    19261 ggctacttcc tgatcgtcca cgacatcgtc gccgaagccc ggcggctggg catcctctgc
    19321 cagggacggg gctcggcggc gaattccgcg gtgtgctacc tgctgaacat caccgcggtg
    19381 gattcgatcg cctacaacct accgttcgaa cgcttcctct ccgcgctgcg ggacgaggag
    19441 ccggacatcg acgtcgactt cgactccgat cggcgcgagg agatcatcca gtgggtcttc
    19501 gatcgctacg gtcgcgagcg ggccgcgcag gtctgcaatg tgatccagta ccggccgaag
    19561 aacgcgatcc gcgacatcag cagggcactg ggctacagcc ccgggcaggg cgacgccttc
    19621 gcccgccagg tggagcgctg gggctggctg acaaccgcgg aagcaaccaa tcgcgaactg
    19681 ccggagccgg tgttcgagct cgcccagcag ctgatggggg cacctaggca cctgggcatc
    19741 cattccggag ggatggtgct caccgagcgt ccggtgggtg aggtggtgcc gatcgagcac
    19801 gcccgcatgg aaaagcggac ggtgatccag tgggacaagg acgatgccgc ctggatgggc
    19861 ctggtgaaat tcgacctgct cgggctgggc atgctggccg ccattcagca ctgcttcaac
    19921 ctgatccgcg acgcgaccgg tgaggcctgg gagctggcga ccattcccaa ggaggagccg
    19981 ggcgtctacg acatgctctg ccgggccgat tcgatcgggg tgttccaggt ggagtcgcgc
    20041 gcgcagatgg gcctgctgcc caggctgcag cccaggcggt tctacgacct ggtgattcag
    20101 atcgcgctga tccggccagg gccgatccag ggcggggcgg tgcatccgtt cgtccgtcgg
    20161 aagctgggcc gcgagccggt cacctaccct cacccgctgc tcgaaccggt gttggcccgc
    20221 acgctggggg tgccggtctt ccaggagcag ctgatgcaga tggcgatggc ggtaggggag
    20281 tgctccggtg aggatgccga cctgctgcgc cgggcgatgg gttccaagcg cggggtggag
    20341 cggatcgagt cgctgaaggc caagctctat gcgggaatgg cccgcaacgg gctgaccggc
    20401 aaggccgccg accaaatcta cgcgcagatt caggccttcg cgaacttcgg tttcgccgag
    20461 tcgcattcgc tcagtttcgc cctgctggtc tatgcgtcca gctggatcaa gctgcactac
    20521 ccggcggcat tcctcgccgg gttgctgcgc gcccagccga tgggtttcta ttccgcgtcc
    20581 accctgacct cggacgcgcg ccgccacggc gtccgggtgc tgcgtcccga ccttgcccgc
    20641 tccggcgtgg acgccggcct ggaactctcc gtcgctcggc cggttgagcc tgtcgagacc
    20701 ccgtccactg gcctaaccag ctgcctgaac ccggagcagc caccggtcgg cgagttcgac
    20761 cccacagcgc cggacgagtc cgtatcccat cgccgggaca ccaatttcgc ggtgcggctc
    20821 ggcctggccg aggtacgggg aatcggcaag gacgtggcca cccggatcgt cactgaaagg
    20881 gaggtgcacg gtgcctacac ctcgatgccg gacctggtcc gacgcaccgg gttggcggtc
    20941 gagcaattgg aggcgctggc cgcggccgat acgttcgcca gcctgggcct gacccggcgg
    21001 caggcgttgt ggctggctgg tagtgccgcg ctggaccagc ccgattcgct acccgggacg
    21061 ctggtggaga cccaaccacc gctgttcggc gaaccgaccc cgaccgaggt gctggccgac
    21121 gacctgtggg cgaccggggt gagtacgacc taccacccga tcgggcaatt gcgcgctgaa
    21181 ctgagcgcgc gcggggtgct cagcactgcc gagctggcca cagctgagcc gggacgccgg
    21241 gtgcaggtgg gagggctggt cacgcaccgg cagcggccgg gaacggcgtc cggcatcacc
    21301 ttcgtcagca tcgaggacga gttcggtctg ctcaacgtgg tctgttccgt cggcgtctgg
    21361 ggtcgctacc gccgggtggc gcgctcggcc cgcgcgctgg tggttcgcgg caaactggag
    21421 cgctcacccg aaggcgtggt caacctgctg gccgaccagc tggaagacct gcccgtcctg
    21481 gtgccgcaca aatcccgcga cttccactga tcgggcatcg aaatgagggc tacgcgggcg
    21541 gaatcggcgc tgataacccg cattttgata gtcgctacgc cgtgcgccgc ttccattcgg
    21601 cgagttgggc agccacttcg gggtagctcg cggcaccgct ggccacagcg atcaccagat
    21661 cgtaggcggc atcgtccggg gcgtccagcg caatgtcgtt gaggccgtag aacacgacga
    21721 tcgcgagcca gccgagccgc ctgttcccat ccgccagtgc gcgattccgg accagggact
    21781 caagcagcac cgcagcctta tcgtccaact cgggataagc ctcgttgccg aagaccgtag
    21841 ccccgggtcg atggactgcg gagtcgagca agccgatgtc gcggacgggg ccgacgccca
    21901 gttcgtcgac caggaacagc acgtcctcca gggagaggtg ctcgatcact tgcccagacg
    21961 atcgagcagg tcggcgtagc ggtcccgagc agcggcggac gactcagcaa cgcgatcgcg
    22021 gcgcagccgt cgcgccgctg cctcgtggat cgcgcgcacg gttgcttcct gccggctcac
    22081 accctcggac tcggcgagca gggcaagcac tcgttcgtct tcagcagtga ggcgtagggt
    22141 catggccatg gcgcaatgat accaaagtgg tatcaagccc cttcgctagg cgttgacctc
    22201 actgccgcgc aggatcgagg ccatcttctt gcccttggcg agctcgtcga ccagcttgtc
    22261 gaggtatcgg atcttttgca tcagcgggtc ttcgatctgc tcgacccggt agccgcagat
    22321 caccccggtg atcagtgcgg cattcggatt gaaggccggt gcctcggcga agaaggtctc
    22381 caggtcgacc tgggtgtcga tgacctgcaa cagctcggcc gcgtcgtacc cggtgagcca
    22441 gctgatcacc tgatcgagtt ccttgaccgt gcggcccttg cgctccacct tgtcgacgta
    22501 gagcgggtag acgctggcga agctcatccg gaacacccga tgtgtcatgc cgggacgata
    22561 acccgtcgca ccggcccgcc gcgaccgtcc gcctacaggc tgtcgcccag atcgcgccgg
    22621 aaagcgtcca ccaggtcgtc cacccagctg ccgatcggtt cgagccggcc gtagaagaag
    22681 cgcaggatct ccggctcctc gatgaaccga aggccaccga tcaggtcccg atggtcgtag
    22741 agccacgcga tccggtcgat cgcgtagttc acctggctga gcgtgaagac ccggcgcggc
    22801 agggcgagcc gcaccagctc catgttcgcc aacggctcca ccccgtccgc gtcccgctgc
    22861 tcgctgagcg tgccccgctc catgccacgc acgccagacg caaggtagag cgcggacgcg
    22921 agcgcaccgg ccgggtactc ctgctgccga acgtgcggca ggaaccggcc ggcgtccagg
    22981 tgggcgccca ggccgccggc gggcgtgatc accggaatgc cgcgcgcgtc cagttcggcc
    23041 accatgtgct cgatgaactg gggaccctgg ttgatcatgt cctcgtccat ggtctcgtcg
    23101 agaccgacgg tgatcgcttc catctcccgc accgacatgc cgccgtaggt gaggaagccc
    23161 tcgtacatcg gcaccaggcc gcgcatctgc ttgtagagcg cctcgtcgcg aatgcagatg
    23221 ccgccgccgc ggccgaagcc cagcttgcga gccgagaaat agatgatgtc gaagcagtcg
    23281 gcgatcgccc gggtgatctc ggcgatagtc atttcgctgc actcgggctc gcgggtcttg
    23341 ttgaagtgca ggttgtcggc cagcagggac gcgtccagca cgctgaggat gccgtgctct
    23401 cggcagacat tggtggtctc gcggatgttc gccaggctca gcggctgccc gccgatcagg
    23461 ttggtgccgg cctcgatacg gacgtaggcg atccgctccg gacccttgtc ggcgatcagc
    23521 gcacgcagtg ccggcacatc gaagttgccc ttgaacagtt cgtcggaggt gacctgcaaa
    23581 ccctcctcgg tgatcagctc cagcacctca ccgccgaccg cagtgacgtg cgccttcgac
    23641 gtggtgaagt ggtagttcat cggcacgatg gtgcccggcc tgaccagcga ctggctgagg
    23701 atgtgttcgg ccgcccgacc ctggtgcgcc ggcaggaagt actccatgcc gaagagttcg
    23761 accagcttgt tgtacagccg ggtgtaggtg gctgagccgg cgtaggcgtc gtccgcgatc
    23821 agcatcgcgg cctgctgctg gtcgctcatg gcattcacac cagagtcggt gagcatgtcc
    23881 aagaagacgt cggcgttctg tagcaggaag gtgttgtagc cggcagcggc tatcgcccgt
    23941 ccgcgctcct cgatcggcag cagagtgagc ttctggatga tcctcacctt gtgcatctcc
    24001 agtgggagtt gctggccgct gtaaaaactc accttcgcca ttgaaagtcc tcaactgtcg
    24061 gtattcgctc ggacccaaag gctattggca ggcgacgata cccttccgcg gtgtctgcgg
    24121 agtgaatcgg acgcggtcag cgggcgagga actcgtcgcc gacccgctgc tggatctcca
    24181 gtgcctccag cagcagcggt gcggcctggc gggcaaggct ggagccgagc accggcaccc
    24241 gcacggtgag ctcgccggtg taggtgagca ccgctccctt accgccgccg accagccgca
    24301 ccttgccgac aagctcggct ggcaggccct caacggtgat gtgcgcggtg ccggcggtgg
    24361 cgccagaaac aggcggtgcg tcccacgcga tctcatcgat cacagccatc gtcggcccgg
    24421 tcagccggcc gaccaccgag ggggtcggca tcacccgccg ggtccgggtg cgcaggccgt
    24481 ccacgctcac ctcgtggtcg agcggttcgc tggccagaca gaccgcccgc aggaagaccc
    24541 ggtcggtcag catggcgaag acgtcgtcga cggtagcggc gaagtcgttg gtggcgtgga
    24601 tctccatgtc gcacatgttg cctggatagg gtggacggca ccaagtcgct gcaaaggagc
    24661 aggttttgtc cacactggtt ccgggtgccc aaaccggcct tcgggagtcg ttgttgacgg
    24721 tcgctgacgg ggacgcggat ctcacggccc tgctgcgctc atatctcgcc ccggtgcccg
    24781 aacccgacct cgtcggtatc gagccggacg cactggccgg actggcccgg gcgcacctgg
    24841 acatgggccg gatccgccgg ccgggggagc acctggtcac caccttcacc cctacggaat
    24901 gggattccca cggctcgctg gtggcgctgc tggtgagtga cgaccaacgc ttcctggtcg
    24961 acaccgtgac actcgaactg acccggcagg agtggagcct gcgtcggctg taccatccgc
    25021 agcctcgaat ccgccgggac gcggacggca acctgctggg cgtaggtagc ggtgatgtcg
    25081 agtcctggat ggccctggag ctctacccgc cgctcgggca ttccgccgag gatctgcagc
    25141 cggagttgga gagcgggttg aggacggcgc tcgccacggt gaccacgacg gtcgccgact
    25201 ggcagccgat gctggcccgc tgtcgcgaag ccgtcgccga actatgggcc gacggcagcc
    25261 accgtccaac ggtgagcgat ggcgtggacc tgctcgactg gctggccgcc gaccacttcg
    25321 tctttctcgg ctacgcccgt tacgtgcccg cgggcgacgg gttggtgccg gtggcgggca
    25381 ccggactcgg cttgctgcga ggcgtcagcg agatcgaccg actgaacgtg ccccggccgg
    25441 gtgaggacga tccggtggtg ctgatgcgcg acctgcgccg ctcaccggtc tatcgccgcg
    25501 cctacctgac ccacctcgcg gtgcggctgt tcgggccgga cggcgcgcta cgcaccgaac
    25561 accgcttcct cggcctgttg ggagcctcgg cctacaccga agccgtggcg agtattccgg
    25621 tactggccgc gaagagccag cggctgctcg ccgactgcgg cttcgaaccc tcctcctacg
    25681 gctggaacgc cacccgccag gtgatcgcca cctatccacg cgaggaactg ttcgaagccc
    25741 gtgtggagga actcgcgccg atcgtggcgg cagtggcagc ccttcgcgaa agccggcaga
    25801 cccgggtgtt ccttcgccgc agtcggtacg gcggcttcac caccgcgctg gtctatctgc
    25861 cacgtgaccg ctacaacacc accacccggc tcgcgctgca gcggatcctc ctggacgagt
    25921 tgggcggact cgagctggaa tacaacaccc tggtcagcga gtccgtgctg gtgcggctgg
    25981 tcttcgtggt gcggatgggt ccggacgcgc ccacgcatcc ggacgttgat cggatcgcca
    26041 tgctggctgc gtccgccacc cgcagttggg gggacgactt caccgaactg gccgccactc
    26101 tgccgagcga gcagcggggg gtcgagttcg gcgaggtcta cgaggccgac cacccgccga
    26161 gcgtggcgat ggctcacctc cacctggcca acgaactcac cgagaccgat gacctgcggg
    26221 tcacgatcgc cgagccaccc gaccctgagg acgacccagc cgaccttcgg ctgaccgtgc
    26281 tcagcctgcg tccgatgacc ctcaccgagg tcatgccgca tctcagcgcg ttgggcgcgc
    26341 agaccgtgga cgagcggccg tatgagtggc aactgcgcga ccgccgggtg ctggtctact
    26401 ccttcggcct tcggctgggc gagggcgaac agtgggacgc gggcacgcgc agccggttca
    26461 ccgcggcctt cgtcgcctcc tggaccggac gctgcgaggc cgaccagctg aaccagctgg
    26521 tggtgcccgc cgggttggac tggcgccagg tcagctggct gcgggcgctg tcccgctacc
    26581 tgcagcaggc cacgctgccg ttcagccagc cgtacctggc gaccgcgctg accacctacc
    26641 cccgcatcgc cgcggcgttg gtcaaggcgt tcgagaccaa gttcgacccg gccgcgttcg
    26701 acgctgtcga tgccgatgcc cgcgaagctg cgttggccgg gttgcagcag aacctttcgg
    26761 aacagctgga gcaggtcgcg agcctcgacc atgaccgcat cctgcgggcg ttctgggcgg
    26821 tcatccgggc cggcgtgcgc accaacgcct tcaccgattc ggcgacgctg gcgctcaaac
    26881 tgctgccggc cgaactggac ctgttgccgc agccgcgtcc agagttcgag gtgtatgtct
    26941 attcgccgcg cgtgcacggc gtccacctgc gcttcggctc ggtcgcccgc ggcgggctgc
    27001 gctggtcgga ccggcgggag gacttccgca ccgaggtgct gggcctggtg aaggcccagt
    27061 cggtgaagaa cacggtgatc gtgccggccg gagccaaggg aggctttgtg ccgcagtcgc
    27121 tgccaaccgg gcgcgccgag cagctggccg aggggcgcgc ctgctaccgg ttgttcatcg
    27181 aagcgctgct cagcgtcacc gacaacatcg tcgacggcgc tgtggtgccg ccggtgggcg
    27241 tggtccgcta tgacggtgat gacccgtacc tggtggtggc cgccgacaag gggacggcca
    27301 cgttctccga tctggccaac gaggtgtcgg tttcgcgcgg cttctggctg ggcgacgcgt
    27361 tcgcgtccgg cggtacgagt gggtacgacc acaaggcgat gggcatcacg gcccgcggcg
    27421 cctgggagtc ggtcaagcat catttcgccg aactcgggct cgatccggac accgacccgt
    27481 tcacggtggt cggcatcggc gacatggccg gggacgtgtt cggcaacggc atgctgctgt
    27541 cgcggcagct gaagctggtg gccgcgttca accacctaca catcttcatc gatcccgatc
    27601 ccaagccggc cgcgtcgttc gtcgagcggg atcggttgtt ccggctgcct ggctcgacct
    27661 gggccgacta cgatccggcg ttgatctcgg ccggcggcgg ggttttccgt cgcgacctga
    27721 agtcggtgcc gatctcgcgc ccgatgcagg tggcgctcgg gctgagcgag tcggtgacga
    27781 agctcacgcc gaacgaactg atcagcgcgg tgctgcgagc gccggtggac ctgctgttca
    27841 acggtggtgt cggcacgttc gtgaagggca gcgccgagac gcacacctcg gcgggggaca
    27901 aggccaacga cggcgtccgg gtgaatggcg ccgagttgcg cgcccgctgt gtcagcgagg
    27961 gcgggaatct cggtttcacc cagctcggac gcgtcgagta cgccgagaac ggtggccgga
    28021 tcaacaccga cttcatcgac aactccgccg gcgtggacac ctccgatcac gaggtgaaca
    28081 tcaagatcct cctcgccagc gaactggccg ccggccggct caccgagcct gatcgggaca
    28141 tgctgctcgc gtccatgacc gacgaggtgg ccgggctggt gctcgcgcac aaccgcgacc
    28201 agaaccttgc tctcgccaac gcgatgttcc gtcggttcga ccttgccggc cagcatgagg
    28261 cgtggatgac cacgctcgag cagtacgggc tggatcgcag gctggaggcg ctgccgtcca
    28321 cgcatgaact gaatgcccgg ctcgccgcgg gccgagcgct gaccagcccg gagttctcgg
    28381 tgctgctgtc ctacaccaag atcgcgttgt accgctgggt gctcgcgtcc gacctgcctg
    28441 aggatccgta cctggcggac cggctggtgc agtacttccc gcaggcgctg cgcgagcggt
    28501 acgaagccgt catgccacag catcggctgg cacgcgagat catcgcgacg gttgccgtga
    28561 accgcttcgt ggatagccag ggcatcactg cctaccaccg gctctcgacc gagaccgggg
    28621 ccggcgtggc cgacatcatg cgggcgcagc tggcgtcccg gtccatcttc aacgcgggtc
    28681 tggacgaggt gcggatccgc cggttggtgg ggctgtccgc cgacgtcggc accgagttgc
    28741 gggtggtgct gctgcggatg gtcgagcggg ccacccgctg gctgctgaac caccgacacg
    28801 gttcgctcga catcaccgcg accgtggcgg aatacggcgc ggagatcgca cagctccgcc
    28861 ccgagctgct cggcctgctc agcggacgga cggccgcgtc cagccaggcg gcggagcagc
    28921 gctggctcga ggccgggctg ccggaggagt tggcgcggaa tctggccacg gctgggcacg
    28981 cccacaccct gctcaacgtc gtggacgtgg cccatcggct ggactgcgac ctgttgcgcg
    29041 cggcgcaggt ccactatcgg gtggctgaag cgctgggcat cgaactgctc agttctgggg
    29101 tcgacgtgct gccacgccag gtgcgctggg atgcgatggc gcgtgccgcg ttgcgggacg
    29161 agttgctcac cgcgcacacc gacctgaccg cagcggtgct gacccatgcc gatgcagaga
    29221 cggacgcagc cacagcggtg cagacgtggc tggacgcgca tccaggcgtg gcgccgcgag
    29281 tggcgatgat ccggcaggtc agcgacggca cgcccgacgt ggcgaggatg aacgtggggc
    29341 tcagccagct gcgcgcgatg ctgggcggat gagtccagcc gatcccgcgg ggacgcgcgc
    29401 ggaagccgtg gtggacgagt ggtgcgcggc ccggctgggt tcagccccga ttacggcctt
    29461 cttcgctgcg cggtcgatgt cgtcggtgcg tggcgtccgg ctggctgacg acaggcaggt
    29521 ggcgctgaag gtgcgggtcg cctcgaagcg ggtgctggcg tgcgcggcgg ggcaccggct
    29581 ggctgctgcg gcggggatcg actgcccgga cctgctggcc gggccggaat ggctgcccgg
    29641 tctgggctgg gcgtccgcgg aggcgtggcg gcctgaaggt gcgttgcggc cgtccggtga
    29701 cgcggcgatc gcctatgccg atctactgcg acggctggtg ggggccttgg gcgatctcga
    29761 cccgggggag ttcgacccgc cgccgccctg ggcgcactac gaccacggcg agcccggacg
    29821 ggtctggccc gcggcgacgt cggcaggttg ggatccggag tcgccggtgg taccggactg
    29881 ggtgcggcgg gtggccgcgg cagctcgtga gcgcctgctg gccgaggacc acggcgcagc
    29941 gacattgccc gctgtggtcg ggcattcgga tctgaacgga ttgaacgtcc ggtggctgga
    30001 cgggccgatc gtgcacgact gggactccct ggcggcacgg ccggagtgcg tcctggccgg
    30061 catcgtggca gtgaaccatg tggagggtcc gaacgcggga gccatcgccg ccgtcgagtc
    30121 gaccgaagcc tgcctgcggc actaccagca cgaccgccaa ttcagcgcag ttgagctgga
    30181 actggcctgg gccgccggcg tgtgggtggc cgcctacaac gcagcgttcg agtacctgca
    30241 cggccggccg ggcgaggtcg tccggcagct gaaggcggac gccgccgagc gcctgcgccg
    30301 agccggctgc tcggactgcc gcgagccttc tcacgattga tcgagctaga cgagccacga
    30361 gcggctcagc cgactcgcgg tgggtcgcgg gcaggcttcg cggccggcct aggtgctgcg
    30421 ggccggtcgg gtcaggctgc tgttcgctcc gggtgccggt gccgggtgtg tctcaaccat
    30481 gcacctgctt gtgggtgtcg agatggtggg gcgaactccg gtagccgatc agcctggttg
    30541 atccggactt gccattggtc gcgccggtag tacttggctg gttcgaccag accgtgatgg
    30601 tggtggcaca gcaggaccag gttcgacagc gcggtcacac cgcccaaata ccagggcacc
    30661 acatgatggg cttcacaggc tgagggtggc gcggtgcagc cggggaacac acagccacca
    30721 tcgcgggcaa tcagggcggc ccgcattgcc ggggtcacca gccggcggct acggcccaca
    30781 tccagcacct cactgccgcc gcgtaggacg acggggacga gatcagcatc acagcacacc
    30841 tggcgcagtt caccagccga caactggttg ccgtcaccga ccacaccggc accagccgca
    30901 ccgttgcgca actggttgta gtcgagtttc acgatcacct tcgccgaacc cacaccgggt
    30961 ttgggtttcg cggtcgccgc atggttcagc aggtgcgtga acgcgtcggc tcggcgttgt
    31021 tcgggagtcg cgttcgcgaa cagggggtcg cgggcttcga ctgcggtgcg gcgtagggct
    31081 tcggtgtggg cgtcgagcag ggtgatgaac tgctcacccg cgagtctggg tagggagcct
    31141 tcgaaccgca ctgacccggc ttgcctccag aatcgtagcg accggttgtg gtgggcggct
    31201 tcagcttcgc gctggagctt cctttccaac aactcgttcg catcggcggg tgcgaccttg
    31261 gccaacacct ggggtgccga cttgatcagc gcgtcggaat ccagggtgtc ggccaggccg
    31321 accagcaact gctcggcccg cgcctgctgc tgctcgtcca actgcgcagt caatccgtcg
    31381 aggacggtgt tgatcgcccg cacctggcca gtactcaccc gtccggacac cgcagcctcg
    31441 cccaacaccg ggtgcgcagc caactgattg gctcgatgca gcgcaccggc agcctcgcgc
    31501 ttggtcaggt tctgatcgat cgccatccac gtcgacatcg gcgtcccagt cgtccgctcc
    31561 gaagcaccgg cgtcatcggc ttcagcaagt aggagtgacg caagagcgtc cagccgtccg
    31621 gtcaagctcc tagcaacggt cgctgccacc aggcggacct gcggcgccag cgccttacgg
    31681 tcgggatcca cccgatccag gacagagcgg atctgcgtca gtgcctcacc caccggcagc
    31741 aaacctgtcc gctcttccat gcatcaagcc aacaagccgc ctctgacgtt tttcgtccag
    31801 aaccgcctca aaccgacgac ggccccggat tctcagcaaa ctttcagaaa taagcgcctg
    31861 aagcggtccc gcgatcagtg gcccagatag cgacccggac ggtgattcag ggccagcacc
    31921 aggttcagca ccactgcgcc ggcggccgag agcagtacat tgcccaccgg caccacggtg
    31981 agcgcggacg cgatgatcag tgcatcgaac gccaactgca cgtagcccgc gcgccagccg
    32041 aattgctcct gcagaatcag ggcgagcgtg ttcaacccgc caaggctgga gtggtggcgg
    32101 aacaacatca gcatcccaac gccggcgagc aggttgccca ccagcgtcgc gtaaaccgga
    32161 tcgatccgca gcgttggcag ggcaagcgga tgcagcgagg cgaatgccga taccagcgcc
    32221 accgagacca gtgtgcgcag cgcgaagttc cagcccttgc ggtgtaccgc caacgccagg
    32281 aacggcagat tggtcaacag gtacagcacc ccgaacggcc aacccgtggc gtagctcagc
    32341 agcaggctca ggccagcggt accaccggtc accgcctgca cgctgtgcaa caggaacaac
    32401 ccgagcgatg ccacgaaagt tccgctgagc agcccgaaca aatcctcaat cgccgtgtgc
    32461 ggcggcgcgc ccgccgccga ttcggcttcc ggctcgcctg agtcgctggc gggatcggtc
    32521 accgggggaa acccgccaga accgcgagca ctcgatcgtt gctaaccgcg tccgcaatcg
    32581 tgacgcggac gccgtcgccc ggataaccgc gcaccaggat gccggcggca tcgaacgccg
    32641 cgaccagccg ttcccgctcg gcttcgtcgc agcgcagcca gacgaagttc gcggcactat
    32701 cgggggtaac ccagccgagc tcccgaatgc ttctcaggag gcgttcccgc tcggcgatga
    32761 ccgtggtcac acgctcgccc acctcgtccg ctgaatcgag tgcggcgaca gcggcagctt
    32821 cggccacgtt gctaaccgag aacgggagct gcgtcttgcg caggccggtc gcgatctcta
    32881 cgctcgcgat cgcgtagccg acgcgcagtc cggccaggcc gtaggccttc gagaaggtcc
    32941 gcagcaggca caggttctgg tactgcgagc cgatcagctg gacgacgtcc ggggcgcttt
    33001 cgccgacgaa ctcgcgatac gcctcgtcga gcaccaccag gacgtggctg ggcacgcgct
    33061 ccaggaagtc gaccagttcg ccttcgctga tcgcagtgcc ggtcgggttg ttcggcgagc
    33121 agagcagcac cagcttggtg cggtcggtga tcgccgcagc catggccggc agatcgtggc
    33181 tctcatccgt cgtcagcggc accttgatcg gcacggcacc ggcgatctga cagagaatcg
    33241 gatacgcctc gaacgagcgc caggcgaaga ccacctcgtc gcccggatcg cagaccgcag
    33301 cgaccagctg cgcgagcacc ccgacactgc cagggccgag agcgaccgca tcggcggtga
    33361 tgccggcgtc gacggcgatc cgatcgcgca gggaggtgct cgccatgtcg gggtagcggt
    33421 tcactctgtc ggcggcggtg gcgatcgcgt ccagcacctt cggcagcggg tggaagtggc
    33481 tctcgttgga cgcgagcgcg gcaacgtcgg ggccggaggc cttccggccg gcgatgtagg
    33541 cgggcagcgc ggcgaccgcc ggacggctct tcgggaactc ggacacgcag gcccctttcg
    33601 tactcaggtg aatcctgagc gctccggccc gggcatgcaa ccggacgggt ccagcgtccg
    33661 atccttgggc tggcggggcg ctggccgcag gggcaggatg ctcggcatgc tggcggacct
    33721 catgcgggac ggaattcgcg tcttcggacg gcgtgcgccg ctgtacgcga gactggccgc
    33781 cggtttcgcc ggccagccgg acgtgctcgc gatcctcgcc gaagcaccgg ccccgcagcg
    33841 gatcccggtc acgttgttcg ccgccatcca cgatctgctg ttggccgatc cggagcagga
    33901 actggcgcag tggtacccga acctgaccgc gacgccgcgc acggacgacc cgctgccggc
    33961 ggcgctcgcc ttctgtgccg agcatcgcgg cgacctggtc gagctggtgc gtacccgcac
    34021 cccgcagacc aatgagatcg gacgctgtgc gttgctgctg gtcggactgg atcgtgtcgc
    34081 caccgaggtg ggtgcgctgg cccagctgga cgtcggggca agcgccgggc tgaatctgct
    34141 ggccgatcac tacgcctacg actactcagg gactcgagtg ggctctgggc ggcccctgct
    34201 cagctgcgaa gtccgtgcag cggcatcaga ccccttgaca gggagcgctc cggctcgact
    34261 cccgcagatc acctcgcggc tggggctgga ccggaacccc attgatctcg acgaccctga
    34321 acaggtgcgc tggttggagg cttgcgtctg gcccgaccag actgagcgct tcgagcgcct
    34381 gcgtgcggcc gtcgaggtgg ccgagcggtt gcgtccgctg gtggtcaggg gcgatgcggt
    34441 cgcaggtctc gccggcactc tcgagaagct gggcgagggt catccggtga tcacgtcgtc
    34501 ctgggtgctg agctacctcg aaccggaacg ccagcaggac ttcgtggctg aactcaacct
    34561 tctgggcagc cagcaggacc tgtcctgggt cagcctggag gagccgagcg cggccactga
    34621 cctcgggtgg ccggacgcgc tggctggcag ctcactgtcg gtcttgcggc tggtgcgctg
    34681 gcgggatggc gctcgtgtcg aggagttcct ggccgagtgc catccgcacg gttggtggct
    34741 gcgctggctg tagggagcaa ctgatcagcg acggacaatg ctggctgaga ctgtcgaagc
    34801 ccctggtcac agatcgtgcg gacgaccctt cgtcaagctc aggaatcgtc aagctcaggg
    34861 atcgttagcc ttcgccgccg ttgcggcgca acacctcgga cagccgctcg gcggccgcga
    34921 tcacggccgg ggcgtgcagg cgtccgggct ggcgggagag ccgctcgatc gggccggaga
    34981 cactcaccgc ggcgatcacc ttgccactgg gggagcggac cggtgccgac accgacgcca
    35041 cgccggcctc gcgctcggcc accgactggg cccagccgcg ccgccggacg gtggccagcg
    35101 cgaccgcgga gaacgacgag ttcagcagtc cacgctggat gcgctccgga tcctcccagg
    35161 cgagcagcac ctgagcagcg gaaccggcgg ccatcgtcag ctgggtgccc accggaatgg
    35221 tgtcccgcaa cccggacgga cgctcggccg cggccgagca gactcggaaa tcaccctgcc
    35281 gccggaacag ctgtgccgat tcgccggtga tgtcgcgcag cctggtgagc accgggccgg
    35341 cagtggcgag cagccggtcc tcaccggcag cggacgcgag ttcggtgagc cgcgggccga
    35401 gcacaaagcg tccctgcaga tcacggctga ccaggcggtg gtgttcgagc gcgaccgcga
    35461 gacggtgcgc ggtgggccgg gccaagccgg tggcggtgac cagtccggct agggtggtcg
    35521 ggccctgctc cagtgcggtg agcaccatcg cggccttgtc cagaacgccg acgccacttg
    35581 agttgtccat aggataagat tgccgtctcg catcatgata tgcaagcact acgctggaaa
    35641 tgagcgaaca gagcaatgga ggtccgatgg gtaagaccct tagtgacaag gtatgggaca
    35701 accatgtcgt gcgcacggca gccggtgagc cggacctgct gtacatcgac ctgcacctgg
    35761 tgcatgaggt gaccagtccg caggccttcg acggccttcg gcttgccggc cgcttggtgc
    35821 ggcgcaccga gctgacgatg gccacggagg accacaacac tccgacgctc aacatcctcg
    35881 cgccgatcgc ggacccggtc tccaagctcc aggtcgacac gctgcgccag aacaccgacg
    35941 agttcggcgt ccgccggcat tcgctgggcg acaaagacca gggtgtagtg cacatcatcg
    36001 ggccgcagct cggtctgacc cagcccggcc tgaccgtggt ctgcggcgac tcgcatacct
    36061 ccacccacgg cgcgttcggc gccctggcgt tcggcatcgg cacgtccgag gttgagcacg
    36121 tgctggccac ccagaccctg atgcaggccc ggcccaagac catggcggtt aacattaacg
    36181 gcgaactgcc cgacggcgtc accgccaagg acgtggtgct cgcgctgatc gccaaggtgg
    36241 gcaccggcgg cggccagggc tacgtggtcg agtaccgcgg cacgaccatc gaaaacctgt
    36301 cgatggaagg tcgcatgacc atctgcaaca tgagcatcga gtggggtgcg aaggcgggca
    36361 tgatggcccc cgacgagacc accttcacct acctgaaggg ccgtccgcac gctcccgacg
    36421 gcgccgaatg ggacgctgcc gttgagtact ggcggacgtt gttcaccgac gcggacgcgg
    36481 tgttcgaccg cgaggtggac atcgacgcca cccagctcac tccgttcgtc acctggggca
    36541 ccaacccggg ccagggcgtc ccgctgggcg ccgcggtgcc gtcgccggag gatttcaccg
    36601 atgcggtgga caagtcggct gctgagcggg cgctggccta catggacctg aaggccggga
    36661 cgccgatgcg cgacatccgc gtggacacgg tcttcctcgg ctcctgcacc aacggccgga
    36721 ttgaggacct gcgcctggcc gcgtccgtgt tgaaggggcg caagatcgcc gacgggctgc
    36781 gcatgctggt cgttcccggg tcagcccggg tgcggctgca ggccgaagcg gaaggtctgg
    36841 acaaggtctt cctcgacttc ggcgccgagt ggcgcgcggc cggttgctcg atgtgccttg
    36901 gcatgaaccc cgaccaactg gccccgggtg agcgcagtgc gtccacctcg aaccgcaact
    36961 tcgaaggccg ccagggcaag ggcggacgca cccacctggt ctcgccgcca gtggctgcgg
    37021 ccaccgctat ccgcggcacc ctgagctcac cggccgacct gtaaggacat ctgaaatgga
    37081 caagttcagc tcacataccg gtattgccgt accgctgcgg cgcagcaacg tcgacaccga
    37141 ccagatcatc ccggccgtct acctcaagcg ggtcacccgc accggcttcg aggacgggct
    37201 gttcgccgct tggcgcaacg atcccgagtt cgtgctcaac gacgccgcct acgccaaggg
    37261 ctcgatcctg gtggccggtc ccgacttcgg caccggctcg tctcgtgaac acgcggtgtg
    37321 ggcactgcag aactacggct tcgccgtcgt ggtcggctcc cgcttcggtg acatcttccg
    37381 cagcaactcc ggcaaggccg gcctggtgat cgcgaccgtt ggccaggacg tggtcgaaaa
    37441 gctctgggag attgccgaga gcgagccggg catcgagttc accgtcgacc tgcgccatcg
    37501 caccatcgag gccgccgaca atgtcttcga gttcaccatc gacgactaca cccggcaccg
    37561 cctgctcgag ggcctggacg acatcggcct gaccctcacc cacgaggacg agatcgcagc
    37621 ctacgaggcc cgccgtccgt cctggctccc gaagaccctg ccggtcaaga ccgcaggctg
    37681 agccaggcgc cgagagaatc ggggacggtt cctttttcgt tgactcgggc gctcccgcgg
    37741 ttcgagccag cgaaactgac tgacgccagg gaatgttctc cggcttgagt cagtttcgct
    37801 ggcgtttgaa gggggatcgg ggcggtcatg cgcaccgatc gcggccgata agctgacccg
    37861 cggatattgg aggtgtcatg gtcatcgagc atgcccagat cagcgtgatc ccgggccgcg
    37921 aggacgagtt cgcggccaac ttcgaccagg cgttggcggt gatccgccag gccaagggat
    37981 tccgttgggc cgagctgcat cgcttcatcg agcgcgaatc ggtcttcctg ctgctggtgg
    38041 gctgggaatc cttcgaggac cacatgaccg gcttccgcga gagtgaactg ttcgcgcagt
    38101 ggcgcgcggt gatcggccca ttcttcgctg agccgccggt cgtggagcac ttcgctgcgg
    38161 tgaccgcagg tgtcggcccg gtggccggct gacgtggctg cggcacgcac ccaggttgca
    38221 ctggtcttcg gcggagtcag cgccgagcat tccatcagtt gcctgacggc aggcagcgtc
    38281 gcgcgagcca tcgacaccga gcgcttcgag gttatcggcg tgggcatcac ccgcgctggt
    38341 gactgggtac gcatcgccca cgccgagctg gtgggctacg aggtggtgga cggacaactg
    38401 ccagaggtcg cgtccgaccg tccggctgcg gtcttgctgg ccgggggaga ggtggctacc
    38461 cgcgacggcg accgcctgct cgaccgccgg ggcgtggacg tggccttcgc gctgctgcac
    38521 gggccctacg gcgaggacgg caccatccag ggcatgttcg aaatgctggg gatgcgctac
    38581 gtcggcgcgg gggtggccgc cagcgcgatc ggtatggaca aggacctgat gaagcgggcc
    38641 atggcagcag ctgggctgcc cgtggggccg tgggttgcga tcacgccgtc gcagtggcgc
    38701 gaccagcgcg aagccagcct ggcgcggatc aacgatttga cctatccgct ctacgtgaag
    38761 cctgcccgtg gcgggtcgag cctggggatc gtccgggtcg aatcggcgga cgagttggtg
    38821 gctgcgatcg aagtggcgca gggtttcgac ccgaaagtcg tagtcgaaca gggtttcgtg
    38881 ggcgtccgcg agatcgaggt gagcgtgctg caggacctgg acggtccgcc gcgggtctcg
    38941 ctgcccggcg agatcctgat gcacacgcca gacgcgttct acgacttcga ggccaagtac
    39001 ctgcccgagg agcaggtcac cctgcaggtg ccggccgatc tgcccgatga cctgcggacg
    39061 gccgtgcagg aacttgcggc acgcaccttc tccgcgatgg actccgaagg gctcgcccgg
    39121 gtcgacctgt tcgtggacgg gtccggcaag ccgttcgtga acgagctgaa caccatgccc
    39181 gggttcacca ggcactcgat gttccccatg atgtgggctg cgaccggcgt ggagtacccg
    39241 gaactgatcg ccaccttggt ggagttggcg ctgaggcgtc cgctgggcct gcgatgacca
    39301 tcggcgggct cggcgagttc gctctgatcg agcaactcac gtccgggctg accgcgtccg
    39361 gcgcggtgcg gctgggcgtc ggagatgact gcgcggtgct gcgcctggcc ggtgacctgg
    39421 ccgtttcgac cgacaccatg gtcgagcacg tgcatttccg gcgtgactgg tccagcgccc
    39481 acgacgtcgg ccggaaggcg gtcgcggcgt gtgttgcgga cgcggaggcg atgggcgcgg
    39541 ttccgttggg tgtcgtgatc agcctcgcgg cgccggccga actgccggtg gagtgggccg
    39601 ccgagttcag cgccggagta caggcggaat gtgccaacgc aggtgcccag ctggtcggcg
    39661 gcgacaccac ttcggcccca ctgatcgtga tcaccgcaac cgtgctggcc gaccttggcg
    39721 gactgccgcc ggtgacccgg gctggcgcac ggcccggtca ggtggtggcg gtggctgggc
    39781 ggatgggctg ggccgcggct ggcctcgccg tgctggcccg gggcttccgc tcgccacggg
    39841 cggtcgtggt ggcccatcgg gtgcccgagc ccccgtacgg gcaggggcgg gtcgccgccg
    39901 aggcgggtgc gtccgcgatg atcgacgtct cggacgggct gctggccgac ctcggtcacg
    39961 tcgcccgcgc gtcgggggtc acgatcgagc tggacacctc gaccctggag atcagcgaac
    40021 cccaacaggc cgtggccgcc gcgatcggcg gtggcgaccc gctggtcttc cagctcaccg
    40081 gcggcgacga ccacgccctg gctgcaatct tcgaagccgg ttcggtgccc acggactggc
    40141 gcgtgatcgg acgcgtccgc gaaggcgacc cgcaggtgct cgttgatggc aggacctggg
    40201 cagggccgac caggggctgg cagcatttcg cgccgctccc ttagcccagg acgcagctgc
    40261 gtcagccaac catttcgggg gctaccgccc ggacattgtc cgagcgccca acaatcatgc
    40321 ggtaacgttt caatcggttc cgtcgaacgc cgtccgcatc tgcccgcggc gccggaccaa
    40381 gccaatggac caccgtccag acctcccttc ggcaataccc gcgaaagccc gctgcatgac
    40441 caaagacctg accgtcggac cacccacccg gatgatcctt tggttcaccc tgcccctgct
    40501 cgtcggcaac ctcttccagc agctctatgc cgtcgtcgat gcgatcgtcg tcggccgcct
    40561 gctcggggtc gaagcgctgg cgtcggccgg cgcgtccgga agcctgcact tcctgctctt
    40621 cggcttcgcc atcggcaccg cctccggcct ggcgatcccg atcgcccgcg cgttcggcgc
    40681 tggtgacctc gccgggatgc gccgggccgt gaccaccggc gtggtgatca gcgggctggt
    40741 caccgtggtc atctcgctgg tcggagtgtt cgcgtccagg acgctgctca ccttgatggg
    40801 cacaccgcct gaactgctcg acgatgcggt cagcttcctc accgtgttct tcagcggcac
    40861 cgtggcgacg gtcgccttca actatctcgc cgcggtcgtc cgcgcgctgg gggacagccg
    40921 caccccgctc tatttcctga tcctggcctg cctgctcaac gtgggcctgg tggtgctctt
    40981 catcggtgga ctccacttcg gggtgggtgg cgcggccgcc gcgaccgtgc tcgcccagtt
    41041 cgtctcggta gtgctctgcc tggtgttgat cgtccgcagg atgccggtcc tgcggctgca
    41101 cgcggacgac tggcggctct cgcgcagcca actggccgag tcgtcgcggc tcggcttgac
    41161 catgggcttc cagatgtcgg tgatcgcggt cggcgcggtg atcctccaat ccggcatcaa
    41221 cggcctcggc gcaacggcgg tggccgcctt caccgcagcc atgcgggtgg accaggtcgc
    41281 agtcgccccg ctggcttcgt tcggggttgc catcgctacc tacgtcgccc agaaccgggg
    41341 tggacgcgag tggcaccgaa tccgggtggg cgtgttccgc acaggtctgg tcgccgtggg
    41401 cgtgtcgttg ctgctcggcg tcgtgatcat cagcttcggt accgcgatcg tcgggctctt
    41461 cgtcggtgac agcgcccccg aggtggtagc gatggcgcac cgttacctgg tcatcaacgc
    41521 cgggctgtac gcgatcctcg cagtgctgtt cgtgctgcgg aatgcgctgc agggcatggg
    41581 ttcggccacc gtgccgaccg tggccggctt catggaactc ctcctgcgca gcctcgctgg
    41641 tctggtgctg gtcgccgagt tcggcttcct cggcgcctgc ctcgccgctc cgctggcatg
    41701 gatcggtgcg ctggtgccgc tggcggtggc atggctgatc gagcggcgca ggctgatcga
    41761 ccgtgaggcc ggaatcgctc cggcgcgcga accgatcccg gattggccgg aactggacga
    41821 cgtgctcacg cccgccggtg cacttgccgc tgactgaggg cgccgtggtt ccgacggcgt
    41881 gggaactcgt cgtccgggca tcgatgacct agccttcgac catggcgacc cgcgcgtctt
    41941 ccccttcgcg gagccgcagc agcgcgacct cgcgcccaag cgcctctagc cggagcaacg
    42001 gaagtagatc cagcagcacc cgcaaaccgg cgagcaagca gccgcagccg tccggcttcg
    42061 tccggttcat gtccggcctc ggccggggca tcgctgcggg ctggcgcgga ctggcgacaa
    42121 tgttcggcag tgccgcccgc ggcatcggac cgaaggtggt gctcgaaccc gaccagcggc
    42181 acgacggcac cgcgctgttc ctgctgctca ccggactcct ggtcgccgcg gagttctggg
    42241 tcgggctgcc cgccgtcctc ggcgactggg tgcacgccgg cacgaccacg ctggtgggaa
    42301 cgtttgccta cgcggtcccg gtgctctgct gggcggtcgc ctggcgcacc tggcggcacc
    42361 ccgagcgcca cggaacggcc ggacgtcagg ctgtcggctg gacagcaggc ctgttcggaa
    42421 tcctcggtct ggtccacatc gccaaagggc tgccccgcgg atcgcagcct gcgctggtgc
    42481 gggaagccgg cggtttcctc gggttcatct cgtcctcgct gaccgtcgat ctactcacgg
    42541 tctggctcgc ggttccgctc ctggtcattg tcaccacgtt cggcgtcctg gtcgtgctcg
    42601 gcgtcccgat gagcgagttg aagagccggt tcaacgcggc gcgcgagcgc ctgaagcgtc
    42661 ccgagcggcc cgaaatcgcc tacggtgccg cccaccgtgc cttcgacacc ccgctggtgg
    42721 acgagccgga actcgatgac gatgaggtgc tgaccgagcc gatcagcgtg tccgcggccg
    42781 cacccaagcc gagactcaag cccgtccagc cgccgctgga agcgccggtg atctcgtcgg
    42841 ggccgctgcg cgccgagcaa cccccgatca gttccgacat cgcctacgta ctgccggacg
    42901 cgggcctgct caaggccgga tcggtaccga aggcacgcac ccaggccagc gacgccgtgg
    42961 tgaacagcct caccgaggtg ctgcgacagt tcgagatcga cgccacggtc accggctaca
    43021 cccgtggccc tacggtcacc cgctacgagg tcgaactggg ccaggcggtg aaggtggaga
    43081 aggtcaccgc gctgggaaag aacatcgcct atgccgtggc ttcggcggat gtgcgcatcc
    43141 tctcgccgat cccgggcaag tcggcgatcg gcatcgagat tccgaacgcc gacaaggaga
    43201 tcgtctccct cggcgacgtg ctgcgctcca accgcgcccg caacgaccac cacccgatga
    43261 ccgtcgcgct cggcaaggac gtcgagggtg gctacgtggt ggcgaacatg gccaagatgc
    43321 cgcacctgct ggtggccggc gcgaccggtt cgggcaagtc gagcttcgtg aactcgatga
    43381 tcacctcgat catgatgcgc gccacgccgg acgaggtacg catgctgctg gtcgatccca
    43441 agcgggtgga gctgacccac tacgagggca ttccccacct ggtcacgccg atcatcacca
    43501 gcccgaagaa ggccgctgag gcgctgcagt gggtggtccg ggagatggac cagcgctacg
    43561 acgacctggc cgccttcggg ttccggcatg tagacgactt caacaaggcg gtccgggcca
    43621 accaggtgaa gctgcccccg ggctcggagc gcgtgcttgc gccctacccg tacctgctgg
    43681 tgatcgtgga cgagctcgcc gacctgatga tggtcgcccc ccgcgacgtc gaggattcga
    43741 tcgtccggat cacgcagctg gcgcgagccg ccggcatcca tctggtgctg gccacgcaac
    43801 ggccgtccac cgatgtggtg acgggactga tcaaggccaa tgtgccttcc cggctggcgt
    43861 tcgcgacctc gtccatgacc gactcccggg tcatcctgga cactccgggt gcggagaagc
    43921 tggtcgggca gggcgacggg ctgttcctgc cgatggggca gtcgaagccg ctgcgcgtcc
    43981 agggcgcgtg ggtcaccgaa gccgagatcc gcgcggtggt gaagatggtc accgagcaga
    44041 tggcgccgca gtaccgcgag gacgtggccg cgccggccga gtcgagcaag gccgtggccg
    44101 aggacatcgg cgacgacctg gaactcgtgc tcgaagccgc tcagctggtg gtgaaccttc
    44161 agctcggctc gacctcgatg ctgcagcgga agctgcgggt cggtttcgcc aaggccggac
    44221 gcctgatgga catcctggag accaggggag tggtcggccc gtccgaaggc tcgaagcccc
    44281 gcgaggtcct ggtcaagccc gacgacctgg acgaagtact ggccgcacta cgggccggct
    44341 gaacccgaga ctgttcagtg cggaggattg tgcaccgtgt tcggtggaca atcctccgca
    44401 ctgaatcgca tgagcagcgc gattctgggt gaggtagcgt ggccggggtg acttcgacgc
    44461 cggtacacct gatcagcctg ggttgcgcgc gcaacgaggt cgactccgag gagttggccg
    44521 ggcgcctcgc ggccggcggc ttcagtcttg tggacgaggc cgacgatgcc accgccatcg
    44581 tggtgaacac ctgcggtttc atcgacgcgg ccaagaagga ctcgatcgac acgctgctgg
    44641 ccgccgccga gcgtaagccg gacggggtgc gcgctgtggt cgcggtgggc tgcctggccg
    44701 agcgttacgg ggccgaactg gcccgtgagc tgcccgaagc ggacgcggtg ctcggcttcg
    44761 acgactatcc ggacattgcc gaaaggctcc gctggatctt ggacggcggt cgccccgagg
    44821 cccacgagcc ccgtgaccgg cgcaagttgc tgccggtgac gccggtggct cgcccggcgc
    44881 gcgcggtcga tgtggccgtg cccgggcatg gctggatccc gcggttccgg ctggccaagg
    44941 gggcttccgc gccgttgaag atcgcgtcgg gctgcgaccg gcggtgcgcg ttctgcgcga
    45001 tcccgatgtt ccgcgggtcc tacctgtcgc gcccggtggc cgaagtcgtc accgaagccc
    45061 agtggctggt cgagaacggc gtccgcgaag ccgtactggt cagcgagaac tccagcgcct
    45121 acggcaagga catcggcagc aacctggagg ccctgctgac cgagctcgac ggtgtgtctg
    45181 gtctggactg gatccgcgtc tcctacctgc agcccgctga ggtgcgtccg tccctgctcg
    45241 aggcgatgca gggcctggag tcggtggtgc cctacttcga cctgcccttc cagcacgctt
    45301 ccgcgaccgt gctgcggcgg atgcgccggt tcggcgaccc ggacgccttc gctgacctgc
    45361 tcgcccggat ccgcgccgac cggccggacg ccgggatccg cagcaacgtg atcgttggct
    45421 ttcccggcga gaccgaggac gacgtggcga tcctgctcga cttcctcgcg tccgctcggc
    45481 tggatgcggt gggggtcttc ggctattccg acgaggaagg caccgagggc gctgggctgt
    45541 ccggaaagct acccgaggac gtcatcgccg agcgcgtgga ccgagtggcg agcctggtcg
    45601 atgaagtgtc cgccgagcgt gcgtccgagc gcatcggcga gcgggtgcag gtgctcgtgg
    45661 agtccgcgat cggctcgtcc ggctggctcg gccggaccgc ccagcaaggc ccggaggccg
    45721 acgggagtat caccctttca gccagtgtcg ggcagctcac tcccggaacg ctggtgtgga
    45781 gtagtgtcac ggcgacggat ggggtggatc tcgtcggtga ggtggtggcc tgaatgactg
    45841 agcgttccgc ggacgcgcgc acgtccattc cggcctggct gcccaacgcg ctgaccgtgg
    45901 tgcgcctgat cctggtgccg gtgttcgtcg tgctgctctt ccttggcccg gccagtttcg
    45961 cctggcggat ggctgcgacg gtcgccttcg gcctcgccat cctgaccgac tacttcgatg
    46021 gcaacctggc ccgcaagcac aacctggtca gcgacttcgg caagatctgg gacccgatcg
    46081 ccgacaaggc gctcactggc gcagccttcg tttcgctgag cctgctcaac gagctgccct
    46141 ggtgggtgac cgtgatcatc ctggtacgtg agtggggcat cacctggatg cgggtggcga
    46201 tgctcaagta cggcgtgatg gcagccgcgg ccggcgggaa actgaagacc ctgctacagg
    46261 ccatcgccct gctgatgttc ctgccctacc cgatcggcca gaccatggcg atcgcaccgt
    46321 tctggggctg gctggcctgg ctggtgatgg gtgccgcgtt cgtcctgacc gcggtgaccg
    46381 gtgtgatgta cgtcttcgac gcgctccggc tgaaggcgga gtcgaccgcc ggttcgaagc
    46441 gatgagcccg aacccgagcg cttccggcgt ggtggcggcc ctgacagcac gtggccagac
    46501 gctggccacg gccgagtcgc tgaccggcgg gctgatcggg gccgcgatca cttcggtgcc
    46561 cggcgcctct gcgatctatt tgggtggcgt gatcagctat gccaccacga tgaaggccgt
    46621 tctcgcgggt gtgccgggcg cggtgctgcg gacgcatggt ccggtggccg cggagaccgc
    46681 ggttgcgttg gcggttggtg tccgggagcg gacgggagcc gactgggctc tggcggccac
    46741 cggtgtggcc ggccccgacc ctcaggacgg acatgcgccc ggcgaggtgt ggctgggcct
    46801 ggcggcgccg gacggggcag caatcgggcg gcggttggac tgcgccggtg cgcgcgagga
    46861 cgtccgcgcg gcgacggtga tcgctgccct cgaactgctc ctgcacgaac tgcagctgga
    46921 agcctgagtg gaccagccgc ttcgcgatcg cgcagcaccg ggaacacatc tgctgggcgt
    46981 ggcgttgtgt caccatcaag tcagagtcgg tggggacggg tagagtcgaa gtcatgatcc
    47041 aggaagtgcg cgagcgtccg ctgttgatgc gggaattgct cggcgagtcg ctgcgtgagc
    47101 tgcgcacctc cgagaatctg acactccgcg aggtgtccgg ccgcgcccgg gtcagcctcg
    47161 gctacctctc tgagatcgaa cgcggccaga aggaagcgtc gtcggagttg ctgaacgcca
    47221 tctgcggcgc gctcgacctg ccgctgtcca cactgctgcg tcgcgtctcc gacaagatcg
    47281 acgcctacgc cggggtcacc cagctgccgc tgcgcagccc gcgcgtcgca gccgcctgat
    47341 gcgcgaaacc gcgctgcggg cccgcttgga gaaacatctg ggctacccgt atgtcctggc
    47401 ctggtcggag acggttgcgc tcgccggcgt ggggcatcgg acggtgacgc aggcgctcgc
    47461 cgccggcgtg ccgtgcgggc agatctggcg agccgcctgg gaagccctgg agctcaccga
    47521 acacgaccgc tgacccgatc acggcggagt tgaagccgtc cgcgtgtcgc gtgcatttcg
    47581 aacacctgtt cgcctaggct gtggatcgtg ggtcgggctt ctccgagctt tccacagccg
    47641 ggatgcgcgc ctgaccgaat gtcagaggcg atgcctatgg tgttgctgac gaggtgttgg
    47701 caggaccaaa gacggaagga actcagatgg cggtggcaga ccgggagaag gcgctggcgg
    47761 ctgcgttggc ccagatcgag aagcagcatg gcaagggctc ggtcatgcgg ctcggtgacg
    47821 agaccaggca gccgatcgag gtgatcccga ccgggtcgat cgcccttgac gtggccctgg
    47881 ggatcggcgg gctgccgcgc ggccgggtgg tggagatcta cggcccggag tccagcggca
    47941 agacgaccgt ggccctgcac gtgatcgcga atgcgcaggc tgccggcggc atctgcgcct
    48001 tcatcgacgc tgagcacgcg ctcgaccccg actacgcggc caagctgggt gtcgacaccg
    48061 acgccctgct ggtcagccag cccgacaacg gtgagcaggc gctggagatc gccgacacgc
    48121 tggtgcgttc cggtgcgctc gcgctgatcg tgatcgactc ggtcgcggcg ctgaccccgc
    48181 gggccgagat cgagggtgag atgggcgaca gccacgtcgg cctgcaggcc cggctgatga
    48241 gccaggcgct gcgcaagatg accggggcgc tctcgggtgc cggcaccacc gcgatcttca
    48301 tcaaccagtt gcgcgagaag atcggcgtga tgttcggctc gcccgagacc accaccggcg
    48361 gccgggcgct gaagttctac tcctcggtgc ggcttgacgt gcgacgcatc gagaccctga
    48421 aggacgggtc ggagatggtc ggcaaccgca cccgggtgaa ggtcgtgaag aacaaggtcg
    48481 ccccgccgtt caagcaggcc gagttcgaca tcctctacgg ccagggcatc agccgcgaag
    48541 gcagcctgat cgacctgggc gttgacaatg gcatcatccg gaaggccggc gcctggttca
    48601 cctacgacgc ggaccaactc ggccagggca aggagaacgc ccgcaactat ctcaaggcca
    48661 acccggaggt ggccgccgag atcgagcaga agatcctgag caagctcggc atcggcgcca
    48721 acggtgtgcc tgacgggatc gacccggtca ccggcgaggt ggagttctag ctttgaccga
    48781 tccggacggc cggttcgacg agccggacga cgaagcggat ccggttggcg tggcccggga
    48841 gattgcgctt cgccagttga ccgtccgtgc gcgcactcgg gtcgaactcg ccagggcgct
    48901 tttacgtcgc aaggtgccga ccgaggtcgc cgagcaggtg ctcgaccgcc tggaagaggt
    48961 cggcctggtt gacgacgcag tcttcgcgcg cgactggctg gctgcggggg aacgccggca
    49021 caagagccga agagcgctgg cggtcgagct gggcgagaag ggcgtggcgc gcgagctgat
    49081 cgacgaggcg gtggccgaac tcgacggtga acacgactat ctggtcgctc gcggctacgc
    49141 ggagaagaag gctgccgggc tcgccaaggt gccaccggac gcacagtacc gacggttggc
    49201 tgcggcgctg gcgcggcgtg ggttcaacgc gtcggtcgtc gcacaggtca cccgcgaagt
    49261 cctggccgaa gtgcgcaccg aggacggctt gcacaatgat tgggacgggg cttagcctgc
    49321 ccttgggtac gcgtgtgaac ccaacctctg agcgcttgac cattaccggg gactgaaccc
    49381 catcgatacc tgcctggctc ggccgggctg atttgccgtc ggcacttggt gctgcgttga
    49441 ccgcttgggt tggctagtgc gcgtccgcac ccgctcaagg aggacgccga tggaactgtt
    49501 gctgggaatc gtccttggcg tactcgtcct cctggtggtg atcctcgcct tcgtgctggt
    49561 gatgctggtg aagggccaac cggcgccgcg tcggggcgcg ccgaacaagc gcggcccccg
    49621 ccaatccgcc cggctacgca gcccgcagcc gctggttggg acgctcgtgc cgacgcaggt
    49681 cccggctgag gccgaggccg aactccgctc gcgccggcag gctgccgatc ggcgggagga
    49741 acttctcgcg cagcgcgagg accggctgaa tcgcttcctg tccgagctgc acacccgcga
    49801 gacccatctg gacgagatcg gggctgcgct cgatcgccgc gaagcggcac tcgacgatgc
    49861 cgagagcgag caggtggaag ccctggcgaa ggtggccggg ctctcacccg ttcaggcccg
    49921 caacgatcta ctcacccagg tcgagaagag ctcgagactg cacgcggccc agttggcgcg
    49981 tgagatcgag tccgaagcaa aacggaacgc ggatgccgtg gctcgccgga tcatcgtgac
    50041 cagcctccag cggctcgccg ctgagcagac caccgaatcc gtggtcgccg ccgtgccgat
    50101 tcccggcgac gacatgaagg gtcgcatcat cggccgtgag ggccgcaaca tccgcgcgtt
    50161 cgagcagatc accggcgtca acgtcctcat cgacgacacc cccgagagcg tgctgctgtc
    50221 ctgttttgac ccggtccgcc gcgagaccgc gcgactcacg cttaccgaac tggtcgccga
    50281 cggacgcatc cacccggccc gcatcgaaga ggtccacgag cgcagccagc gccgggtgga
    50341 ggagttgtgc atccgggctg cggaggacgc gctcatcgag atgggcatca ccgaccttga
    50401 cgccggcctg ctgccgtacc tgggtgccct gcagtaccgc acgtcctacg gccagaacgt
    50461 gctgcgtcac ctgatcgagt gtgggcacat cgcagctctc ctggccggcg aactggggct
    50521 cgacgtcgac acctgccggc gtgccgcgtt cctgcacgac atcggcaagg cgctcaccca
    50581 tgaagctgag ggctcgcacg ccaaggtggg tgccgacctg gctcgccgcc acggcgagca
    50641 tcctgacatc gtccatgcca tcgaggcgca ccacaacgag gtcgagccgc gcacagtgga
    50701 ggcagtcctc acccaggcgg cggacgcgat cagcggcagc cgtccgggag ctcgccggga
    50761 gagcctggag atctacgtga agcggctcca gcgattggag gacatcgcca aggagcacga
    50821 gggcgtcgac aaggtgttcg ccatgcaggc cggcagggag atccgggtga tggtcacccc
    50881 agaactggtc aacgacgccc aatcgctggt gatcgcacgt gacatcgcca agcgcgtcga
    50941 ggaggaactc acctacccgg gcaacatcaa ggtgaccgtc gtccgcgagt cgcgggccgt
    51001 ggcgctcgcc aagtagcccc gtcccggacc tgtccggcag ccccgcatcc gcctaccacc
    51061 tggccgcccg gcgccgcgcc tcgatggctc agcctcgctc catgccgaac aacaatcagc
    51121 gttccttggg taaagtagct cgtcatgcgc acgttcgaag tggtcaccta tggctgccag
    51181 atgaacgtcc acgactccga acggatcgcg ggcgtacttc tggacgcggg ctaccagccc
    51241 gctgaggctg gatcccaggc cgacgtggtg gtgttcaaca cctgcgcggt tcgggagaac
    51301 gccgacaacc ggctctacgg gaacctcggt catctcgcgc cggtgaagca ggcgcacgcc
    51361 ggcatgcaga tcgcggtcgg cggctgcatg gcccagaagg accggcacac ggtcgtgcag
    51421 cgcgcgccct gggtggacgt cgttttcggc accaacaacc ttggctcgct gccggtgctg
    51481 ctggagcggg cgcggatcga atccgagtcc caggtggaga tcctggagtc gcttgagcgg
    51541 ttcccgtcca ccttgcccac ccggcgcgac tccgcctatg ccgcgtgggt gtcgatcagc
    51601 gttggctgca acaacacctg caccttctgc atcgtgcctg cgctgcgcgg caaggagacc
    51661 gatcggcgtc cgggcgacgt gctggccgag atcaaggcgc tcgtggccga aggcgtgcag
    51721 gagatcaccc tgctcggcca gaacgtgaac agctacggcg tcgagttcgg cgatcggggc
    51781 gcgttcgcca agctgctacg ggcctgtggc gagatcgacg gcctggaacg cgtccggttc
    51841 acctcgccgc accctgcggc cttcaccgac gacgtgatcg cggccatggc cgagaccccg
    51901 aacgtgatgc cgtcgctgca catgccgctg cagtcgggct cggacgcggt gctgaagacg
    51961 atgcgccgct cctaccgcag cacgaagttc ctcggcatcc tcgaccgggt acgggcggcc
    52021 attccgcacg ctgcgatcac caccgacatc atcgtcggct tccccggcga gaccgatgcc
    52081 gacttcgagg cgaccatgga ggtcgtccgc gcgtcgcggt tctcggcggc gttcaccttc
    52141 cagtattcga tccggccggg gacgccggcc gcgacgatgc cagaccaggt acccaaggcc
    52201 gtggttcagg agcggtacga gcggctggtc gacctggtcg gcgagctggc ctgggccgag
    52261 aactcaaagc tcgtcgggca acgggtggag gtgctgttcg ccgacggcga gggccgcaag
    52321 gacgccgcca cccaccgcat gtccgggcgg gcccgcgaca accgcctggt gcacgtggcg
    52381 gtgccggatg atccggcgct gcggccgcgt ccgggagacc tcgccgaggt agagatcagc
    52441 tacgccgcgc cgcaccacct gaacgccgat ggcggcctgc tgagcctgcg gcgcacccgc
    52501 ggcggcgatg cctgggccgc acgtcaggcg cctcccgagg ctgaagctgc gcccgggcgg
    52561 acggtcggcc tggggctgcc gaagctggtc cgggcataga ccgaggggcc gaccagcttg
    52621 gctcgacccc tcgggtttcg tcgttgtgcg ggaagtctgt cccgctccag atccagtgga
    52681 tccgtatcgc tcagttaccg agttggtcct tgacgaactc ctgagcctgg tccacctgtg
    52741 ccgcgtactg tccgccggtc ttctcatcaa tgaagtcgcc aacctgctcg acgccttcct
    52801 tgatctcagc tgcgtggtcg gccacggcat ccttgatctg ttcgacgatg tccatgtgac
    52861 ctcctttctg tcccggcgcc aggcctatcc cggctgcatc atccattgtg tgccgcaaag
    52921 gacgcgttgc caagggaaat caccgaccgg ttgagacaat gttccacgtg gcgattccgg
    52981 cagtggcact gaatgggccg acggccagcg gcaagaccgc ggtggcggtc gaggtggcgc
    53041 gccttctggc ggacgcaggg ctggcggcag aggtggtgaa cgccgattcg atgctggtct
    53101 atcgcggaat ggacatcggc accgcgaagc ccacggtggc agagcgcggc ggcgttccac
    53161 atcacctggt cgacatcctc gacgtcaccg agaccgcgag cgtcgcggac ttccagcgcc
    53221 ttgccagagc agcgatcgcc gactgcaggc agcgcggcgt ggtgccgctg ctggccggcg
    53281 gttcggcgct ttatgtgcac gcgatcctgg acgccttcga gtttcccggg acggatccgg
    53341 ccattcggtc gcggctggaa gccgagctgg acaagttggg ttcccccgcc ctgcatgccc
    53401 ggctgactga gttgaacccc gaagccgctg ccgggatcct gcccggcaac gcccgacgga
    53461 tcgtccgggc gctcgaggtg atcgagctga ccggcacgtt cacgtccgtg ctgccggaat
    53521 ggacgtacgc actcgacggc gtgctgcagt tcggtctgga ggtgccacgt gaggtgctgg
    53581 acgagcggat cgccgcacga gtggaccaga tgtgggccga cggcctggtt gcggaggtcg
    53641 agaaactcgt ggaccggggc cttcgcagcg ggcggacggc gtcccgcgcg ctcggctacc
    53701 gccaggtgtt ggcgttcctg gacggtgagc tcaccgagtc cgaggcccgg gaggcaacga
    53761 tcacaggaac ccgcagattc gcccgcaagc agttgggctg gttccggcgg gatccgcgga
    53821 tcgtctggtt gccggccggg ccgcagagcg cgatcacgat cgcgtcggtg gtaacggata
    53881 aggtgcttgg cggaccacag gaggcgtgat ggacatcgag ttcgccaagg ggcacggcac
    53941 ccgcaacgac ttcgtgctgt tcgccgaccc cgacaacctc tacgacctga ctgccgagca
    54001 ggtgcgctac ctgtgtgacc gccgtgccgg gattggcggc gacggggtgc tccgcgcagt
    54061 ccggaccaat caggtgtcgc agtgggccgg cagccgggcg gactggttca tggactaccg
    54121 caacgccgac ggctcgatcg cggagatgtg cggcaacggg ctgcgggtct tcctgcgatt
    54181 tctcgccgag gaggatctga tcgatccgtc cggggtgacc gcggtggcga ccagagccgg
    54241 cctgcgcagt gggtggctgg tggacgacgg ccggatcgca gtcagcatgg gcgcagtgag
    54301 catcggtgac caggtgacga tcagccacgg cggacgcacg tggccggcga cggccgctga
    54361 tgtgggcaat ccgcacgccg tggtcgaggt ggcctccgcc gcggaactcg acgggctgga
    54421 tctcagcctc gcgcccactt ggtcgcctgc ggacgctttc ccgaacgggg tgaacaccga
    54481 gttcgtcctt cgcgaaggcc ctggccggct tcggctgcgg gtgttcgagc gcggggttgg
    54541 cgagacggac tcctgcggca ccggcgtggt cgcgtccgct gcggtactcg gcggtgcgca
    54601 gaactgcgcc gtgcaggtgc tgggcggacg cctggaggtg cgactgggcg atccggacgc
    54661 ggtcctgatc ggccccgccg cggtcgtggc gcgcggtcgg atcgacctgc cgagctaagt
    54721 tggatggacg atgactgaag aagaatcgct gatcgaatcc tccgttgacc tgcaggctcg
    54781 gcactcgctg cgccgggtgg ccggcatgtc cacccagctg gccgacatca ccgaggtgga
    54841 gtaccgcgag ctccagttgg agcgggtcgt gctggtcagc gtctggacca gcggcaccga
    54901 agctgacgcc gactacgcca tggccgagct gaagttgctg gcggagaccg cgggttcgca
    54961 ggttctcgag gggctggtcc agcggcgcag ccatcccgac ccggcgacct tcatcggccg
    55021 cggcaaggtc gacgaggtac gcgaggtcgt gctggcgacc ggcgccgaca ccgtgatctg
    55081 cgacggcgaa ctcgagccgg cccagctgcg caaccttgag gaccgggtcg gcgtgaaggt
    55141 cattgatcgc accgctctga tccttgacat cttcgccacc cacgccaaga gcgcagaggg
    55201 caaggcgcag gtcgagttgg ctcagttgca gtacctgcgg cagcggctgc gtggttgggg
    55261 cggcaatctc tcccgccagg ccggtggtcg cgcagcggga ggtgccggaa tcggtggtcg
    55321 cggtccgggc gagaccaaga tcgagaccga ccgtcggcgg atcaagaccc gcatctcgat
    55381 cctgcgcaag cggttgcgag agctggacgc cgtccgtgac accaagcgtt cccagcgcac
    55441 ccgcaaccag gtgcccagcg tggcgatcgt cggctacacg aacgcaggca agagttcact
    55501 gctcaaccgg ctcacgggcg ccggtgtgct ggtcgaggac gcgctgttcg ccaccctcga
    55561 cccgaccact cgccgcaccc agaccacgga cggccgggtg tacacgctca ccgacacagt
    55621 cgggttcatc cggcacctgc cacacgacct ggtggaggcg ttccgttcca cgctggagga
    55681 gtcggttaac gccgacctgc tgctgcacgt catcgacggc tccgatccgg acccgttcgg
    55741 ccaagtcacc gccgtgcgca ccgtgctggc cgagatcggt gcggcgaatg tcgccgagca
    55801 attggtgatc aacaagatcg acaccgccga gccgaacgaa ctgctggcgc tccggggtcg
    55861 cttcccggac gcggtgttcg tgtcggcccg caccggggca ggcatcgccg acctgctggc
    55921 tcgcgtggag gagcggctgc cgcgaccggc gatcgaggtg cacgcgttgg tgccgtacga
    55981 ccggggtgac ctgatcaaca agatccacct gaccggtgag ttcgtctcca gtgagcacac
    56041 cgagaacggc accgcggtgg tcgcgagggt gaacgccgac ctcgccggcg agctggcccg
    56101 ctacgccgcc gatggctgag gcagttccca tcggtcaact cgccgaacag atcctcgagg
    56161 ccgcgatcgc ggggataggc ggtgcggcgc gtcccggtca ggggaggatg gcgaccgagg
    56221 tggcccggac ccttgacacc ggcgaacacc tgttggtgca ggccggcacg ggcaccggca
    56281 agtcgctcgg ctacctcgca ccggcactcg cagcgctcgt gcaacagcgc gaggcgcggg
    56341 tggtcgtggc gactgccacc ctggcactgc aggcccagct ggccgtcaag gacataccgg
    56401 cggcggtcgc cgcgtgcgcc gaggtcaccg gccagcaggt cagccacgcc gtgctgaagg
    56461 gccgcagcaa ctacgcctgc ctgctgcgcg tccgcgacgg ccagggagca gagcaggacg
    56521 cgctgctgcc tgagcccggt gaggccactg gtgtcggcgc cgaggtggtt gaactgcgcc
    56581 gctgggccga gaccgaggcc gagcacggca gcctggccga ccgcgacgac gccccggccc
    56641 acacgccgtt ggcctgggcg caggtatcag tgccggtgcg tgagtgcgtt ggcgcacagc
    56701 gctgccctta cggcacggag tgcttcgtgg agaagtccag ggagcgtgcc cgggcgagcc
    56761 agttggtggt gacgaaccac gcactgctcg cagtcgacgc gctccacggc ggtaccgcgc
    56821 tgcccgagta cgacggcgta atcatcgatg aggcccacga actggtgtcc cgcgtgaccg
    56881 gtgcggcctc ggtggagctg agcccgcagc aggttgaacg cgtggccaaa cgctgtctgg
    56941 cctgggtggg cgacgaagtc ggcctggagt tccttgatgc ggcggacgcg ctgcagcttg
    57001 gtctcgacgc ggacgcggtc ggccgggtgc tggacccgtc cgcgcccgtg ctggaggcgc
    57061 tccgggtggt gcggatcgcg gccaggcacg cagtctccgc cctcagcggg ggcagcgacg
    57121 aggagccaga gcggcgtcag gcacagggtg cgacgcagga ggtcttcgac atcgcggaac
    57181 gcatggccaa gctggaggag cacgacgtcg tctggatttc cgagtcggac cgcttcggcc
    57241 gacaggtgaa ctgcgctccg ctttcggtgg ccggtctgct gcgcagccag gtgttcgaca
    57301 accacccgag cgtcctgacc tcagccacac tgaaggtcgg cggcgacttc aaagcggtgg
    57361 cggcgtcgct cgggcttggc ggcgagggtg gttcgtggcg cggtgaggac gtcggctcgc
    57421 ccttcgacta ccggagccag gggattctct acgtcgcggc tgacctcgcg ccgcccggcc
    57481 gcgacggcat cagcgcggag acgctcgccg agatcgccga gctgctctgg gctgccgagg
    57541 gccggacgct cggcctgttc gcgtcccagc gggctgcaga ggctgcggcc cgctactgcc
    57601 gcgaacacgt gcccgaccgg ctggtgctct gccaggggga cgcgcacctg gcccggctca
    57661 ccgcggagtt catcagcgac ccggaagcct cccttttcgg caccctctcg ctgtggcagg
    57721 ggatcgacgt gccgggcgac acctgtcggc tggtgatcat cgacaagatc ccgttcccgc
    57781 gcccggacga cccgctgatg caggcgcgtc agcaggcagt ggcggacgcc ggcggcaacg
    57841 gcttcatggc ggtggccgct acccatgccg gtctgctgct cgcgcaaggt tccgggcggc
    57901 tgatccgcac gctcaccgat cgtggtgtgg tggcgatcct cgactcccgc ctggtcaaag
    57961 cgcgctacgg cagcttcctg cgcgcgtcga tgccggactt ctggatgacc accgatcgcg
    58021 agatcgcgat tggatcgctt cgccgcctgg ccgaggccgt cgaaagcccg aatcagtagc
    58081 cgccggaccc agctggcacc accagccccg tcccgaaaca acccacacaa agtggacagt
    58141 ttggtgccgt ctgcgcgaga aacggtccac tttgttggc
//
LOCUS       DAOPRQ010000003        53944 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_458_length_53944_cov_12.036018, whole genome shotgun sequence.
ACCESSION   DAOPRQ010000003 DAOPRQ010000000
VERSION     DAOPRQ010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 53944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 53944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53944
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_458_length_53944_cov_12.036018"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(12..85)
                     /locus_tag="PKJ61_00580"
     tRNA            complement(12..85)
                     /locus_tag="PKJ61_00580"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(51..53),aa:Gly,seq:ccc)
     gene            583..1260
                     /locus_tag="PKJ61_00585"
     CDS             583..1260
                     /locus_tag="PKJ61_00585"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOC12360.1"
                     /translation="MSKEERAVTIVELVTQTMSVLNDGEKGELAVCHGLAKATASDAA
                     VLIGTDFTRRVRVHAVWPNHRAAAPLAELVVAAHGHPAGDHPVLGEVLTLTFGAPNES
                     HPHSNARTIVLSRRARFTPEARMFLTEAGPALQLLLPHVGDACLAAEQSRAAADAAAA
                     LNLTSREVEVLQLLSEGLLARTIAARLGLSPRTVHKHLSNVYTKLGVHDRLVAVSFAR
                     DRGLISA"
     gene            complement(1291..1686)
                     /locus_tag="PKJ61_00590"
     CDS             complement(1291..1686)
                     /locus_tag="PKJ61_00590"
                     /inference="COORDINATES: protein motif:HMM:NF015953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HOC12361.1"
                     /translation="MIRMLIRGGIYFGLAVVGLLLAALIPGVVLRPGGFLVAVLVFTL
                     AQSLITPLAMRLSRKAGQALIGGMGLISTGLALLVASLFEGGITFDGGALPWLGATLV
                     VWLVTAIGGWLLPLWLLKDKVAQAKARRK"
     gene            complement(1708..2538)
                     /locus_tag="PKJ61_00595"
     CDS             complement(1708..2538)
                     /locus_tag="PKJ61_00595"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HOC12362.1"
                     /translation="MLRRVVFPMLATLAVIGLVVWVIGTLPRWWPGLLPAPTASSTTA
                     AAEATKDVSGFAKAKVLRVVDGDTLDVRLDGAETKLRLLNIDAPETAHDGDPAQCLAK
                     AAKAKLAELAPNGATVRIENYGHDRFGRTLVGLYSERGELLNAQLVGAGLAAPFVVNG
                     QVNLLRPVRAAQAEAAAAGSGLHEPKGCTVPGRVNAAEAAVAELPASATGPDAVAAAK
                     AAKAVQGDLQVLASDLTGDARYVPVDALTKDEVSALSVRVSAASKAVAQVLARLTAKR
                     "
     gene            2650..3273
                     /locus_tag="PKJ61_00600"
     CDS             2650..3273
                     /locus_tag="PKJ61_00600"
                     /inference="COORDINATES: protein motif:HMM:NF021443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1707 domain-containing protein"
                     /protein_id="HOC12363.1"
                     /translation="MANPIEPSDVGPAVGGHIAVSDADRRHVITLLNSAQAEGRLTPL
                     ERDERIAAAEAAEIFDDLVPLTRDLVSPTGPAARPVVNYDEVHASGAADQIVAIFSGA
                     TRKNAWRVRRNTSIMAVFGGVELDLTRATFEAHELSFNVFCLFGGVEVTVPPGTDVDS
                     QVIAVFGGTEVGRLTPPDPALPKIVVKGFCGFGGVQVRNPKAHKDRN"
     gene            3298..3783
                     /locus_tag="PKJ61_00605"
     CDS             3298..3783
                     /locus_tag="PKJ61_00605"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HOC12364.1"
                     /translation="MTDLFTPTATWQRLPERCATARRVSAAVSNLIATAVVALPLGFF
                     FDWRWAAGLAVAGLAWTLWRVIRAGRWVRNFGYAERAEDLLITQGLWFKRLTAIPYGR
                     MLSVNVESGPLDRAWGLATVELVTASVQSGAQIPGLAQADAAALRDRLIAAGESQALP
                     L"
     gene            3930..>5294
                     /locus_tag="PKJ61_00610"
     CDS             3930..>5294
                     /locus_tag="PKJ61_00610"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HOC12365.1"
                     /translation="MSERPHPLTPFVRGWVLILAIILGVGRDLLQHQPDEGPLPPLEW
                     IASGIGAAVLIAVGIGFVSWRFTRFVIDEDELRVDTGAVFKRSVRVAFDKVQSIDVVQ
                     PFAARLVGLAELQIDVGSQERVRLRYLKLTRAYALRDYLLARARGNRVAATEHVQASS
                     VLADLTEHDEVLVQVPPQTLLLAAVTSHEFFSIIGGGLVSAAVVTYLGWGWGALAILI
                     PVASGLFSFVSRRIIGQFNYTLSRREHGLRVSRGLTSLTSQSLPPRRVQAAQITQSPI
                     WRKLGLYRLDIAVIGWGGISRDENDTGVSTILLPAGSIDQVRAALSAIWPRTNFETIA
                     LQQPPKRARWLHPFAVPFMRWGHDDQLTVTRHGWLVRTWHLVPHARTQSVRITQGPLS
                     RRLRLAELEFHTAGGTVQARARGLDADSVRDLQAELLARVHHLPELDLSEPTPQPEPS
                     ESLGD"
     assembly_gap    5295..5394
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5395..5947)
                     /locus_tag="PKJ61_00615"
     CDS             complement(<5395..5947)
                     /locus_tag="PKJ61_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007078030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOC12366.1"
                     /translation="MTSWLVTGGAGYIGAHVTRALQQRGLGPVVLDNLSSGLTAFVPD
                     GVPFVEADILDTATVTAALRDHGCTGVIHVAGYKYAGVSVQRPLHTYAQNVTGTASVL
                     EAMAAVGVRNLVFSSSAAVYGTPTTELVTEDSPNHPESPYGESKLIGEWLINDVARAD
                     DGFRGVSLRYFNVVGSATDEVYDA"
     gene            6035..6799
                     /locus_tag="PKJ61_00620"
     CDS             6035..6799
                     /locus_tag="PKJ61_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016666290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOC12367.1"
                     /translation="MQNRAVLGIDIGGSGIKGALVDLAAGDFASERVRIGTPAESTPA
                     NVADVVAEIVEQFADQMGDGPIGITIPAVVTHGVTRSAANIDKSWIDAPAEQIFEERL
                     GRDVVLVNDADAAGVAEVVWGAAKGNDGLVIMTTLGTGIGTAIIHRGVLLPNTELGHL
                     EIDGHDAESQAAASVRENLKLSFDEYVPRLQRYYERLEALFWPDLIVVGGGISKNADK
                     FLPKLKLKTPIVPAVLRNKAGIVGAAWLAEQKAESH"
     gene            complement(6907..8541)
                     /gene="argS"
                     /locus_tag="PKJ61_00625"
     CDS             complement(6907..8541)
                     /gene="argS"
                     /locus_tag="PKJ61_00625"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016666318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOC12368.1"
                     /translation="MLSLPTLLSERVAAVAGIDPELRPATRPQFGHYQTNVALRLANA
                     DGRPPREVAASLVEKIQLDDLCEPLEVAGPGFINLRIRADVLAAVASQVLTEPAAGIT
                     PAVQPKTVVIDYSSPNVAKQMHVGHLRSTIIGDCFTRVLRAQGHTVIPQNHIGDWGRQ
                     FGMLVEEILDEGLDVSTLDLVGAEELYQRANKHLKESEEFADRARARVVLLQSGDVET
                     RRIWQQLIDVSLAGFNATYQRMNVLLTNADLAGESIYNADLPVVAADLESRGIAVVDD
                     GALVVFVDGFPAPAIIRNAQGGFGYSCTDLAAIRHRVGDLGADRMIYVVGAPQAFHFE
                     QVFAVARIAGYLPETTSAEFVGFGQVLGADGKKFSTREGTAVTLNSLLDAAEKEAAPP
                     IALAAIKYADLSSGLQKDYTFDAERMVATTGDTGPYLQYAHARVNQILRKAEAEGLTY
                     AGVTVLTEPAEHQVALLLSRFGEVVAAVGETLQPHRLCGYLYELAGALSSFYEQCPVL
                     KSEGDVRASRLALCAATKKVLASGLDLLGIQAPERM"
     gene            complement(8731..9366)
                     /locus_tag="PKJ61_00630"
     CDS             complement(8731..9366)
                     /locus_tag="PKJ61_00630"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElyC/SanA/YdcF family protein"
                     /protein_id="HOC12369.1"
                     /translation="MVWLRSGGGTRCQLAFGLGVAAVPLAAVLGLGATVHLITRSRIV
                     SLERAPHTPVALVLGAEVFADGTPSAFLRARLDLAAALYRRGLVERLLLSGDGRSRFY
                     DETGGMRHYLEGLGIEPDALLVDPAGLDTYASCRRARDVYGVRRMIVVSQRYHLPRAL
                     AICRAIGVDAWGVGDETARTSSRTWAYGVRRELAANLKLVWDLLTRNSGSG"
     gene            9554..11071
                     /locus_tag="PKJ61_00635"
     CDS             9554..11071
                     /locus_tag="PKJ61_00635"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HOC12370.1"
                     /translation="MRKRAGRLGRVLAGFAVLSLVATLLTLLQSPRATAEPVLPSAPD
                     TVTVSQRSTMVIASWPVVAGADGYTVELDTMPTFSAPRRVVTADTMSVVTGLTPDTGY
                     YLRIASWAEDPSTFGSWSSLYPFSTGPQEFAKLPPVVKTDSLTSTSITAEWTDVDKDL
                     SYEVALGKQPDDLTATKQVKDQTATFDELEKVTKYYVSVRAVDEAGAPVTAWSDPVDQ
                     KTAKELPLRVGSYNILCENCSGGKATWASRRGPLVDAIQEQDLDVLGVQEASIGSIPG
                     GGAQYMDLINRLGKPYKLTEYGRKISPDVRIVYNSERVELVKQGTIALPRGDSARFLN
                     WAVFEQKSSGKRFFFTNTHLEPKDGKKYWNTRNRQAKAVVAATKKLSGDLPVVSVGDY
                     ASTKWDDPSNGPYDIMQSAGYKDPLGNAARSRNSAPGEFVEKRINTSYASLNMYKRQA
                     RNFAGDVNGSNTDYIFVSTMRVPEYEVVVKVNSAGQFVGVIPSDHNLIRATVLLP"
     gene            11111..13261
                     /locus_tag="PKJ61_00640"
     CDS             11111..13261
                     /locus_tag="PKJ61_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC12371.1"
                     /translation="MAKLVDTPRPPAAVGLSTAQGFRREGFGSARWPGYGRDPAGHFQ
                     EFQLMLPKSSVTVRLRAALVGLALVLGLVVANPAPAEAAPKAPSGLTRASYSNNAVRL
                     TWKAVKGAAAYQVKYASNSKLKKASYVTVAAPVAEIGGLKASKNYYFKVKALKADGKA
                     LTKYSKTKKIKTSSTKAYTVLSPSGLALKANYGDQVNLAWTAQGSTNRYRVNWSTSKS
                     MKKAKAVYVTGPNAAIGGLSRSKNYYFTVQVSSAGTKAISPATTVLKVKTRKTVTAAS
                     PMATVSRVEPTAFTVSWKPARTAPAYEVVYSATNWADSSRVVAAGTSVKVQGVFAKST
                     YQVKVRALDSANKPVGGFSTVASVTTPSGEESLRLASYNVRCFDCKPKSQSGTPIVDL
                     PWTQRRSLVAQNIGEANPDVIALQEAQQAELGDGSKLNQYQDLLLAMNEQLGGVWKLT
                     NDREYNCVKSTTPTACEPKDQGASKGVRIAYRIDRLAILRENGVDRTGSMLLPTAPAD
                     GSGASLKASERYMVWAFMQQLTTKKTFLMVDVHLESRDDTTSSSAGDAGCGDTANPIC
                     GKLRVEQAQAIVAKIAQLNTDGVPVVVAGDFSSSRSEASKLFPSDVFTASGLVNPLGG
                     IEKYYDPDAVPANTRINIVYNSVNQLERTPRRYGSDPAKNNTYNGAYNDYILTSPMAV
                     YDFEQLVHLNGDGTYADQQFASDHNLIRMTVRLP"
     gene            complement(13421..15922)
                     /locus_tag="PKJ61_00645"
     CDS             complement(13421..15922)
                     /locus_tag="PKJ61_00645"
                     /inference="COORDINATES: protein motif:HMM:NF016357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HOC12372.1"
                     /translation="MAPKYNRLETLIRRTRRSRFAFLYTGYLSGRKAYRAVVNRFTGK
                     QVGWVAWMQSGRWVGTTSFELVGWAYERGTGSNDVVPTVTVTCTNDRTKQKIRAKATQ
                     RYDAAANIRARLMPVDYGNYAFVATLDLASALAGEIGDSWTVRVTVDDTERKLTGTLK
                     NRAKTAAGAFLVARTAGDRQLEPVWTGKETGLKFLLRQPPVVAETASFDGERVELVVL
                     APEFEVARAALVEADKAPLAVESLGAGRYRLSATLATPTAVANVAEPDLEKNNPEDLG
                     ASLAVHDWLVQVWDSAGNKATVASGLDERDAEWTPGQARYAYTGPGSALLIRDTAAML
                     LVDDVELVDEPLSLRYTGHALGRLEGAKLVLTGPRQDLEVELTIDGDRFTAVAPLLIS
                     AWGGPKLPPQTGAYVLRGRTAAGAWLRVGATSALIQRTPLRTMGELIKLRVGVASGRR
                     VTCLFSAPLQDQLVGDYQQARLKAEYHARTFEPQEAVYFESFNGRAATCNTYALDREV
                     ARRFPELKRYWSVLDYSVAVPEGAIPVLKGTLQWWDVRSTCRYIITNEWIRQKFKHQP
                     FQVVLQTWHGSMFKRIGLDRPNFSKDEQTFLAYERVKWDVLLSQNAHSTAIFRSAYAW
                     DGPIWEEGYPRNDDMSTKSGEPIRELLGIRPDQKAVLYAPTWRDDAEETLVDFLDLPD
                     MARKLGDDYVILLRGHSRTLRTDGNVRVPGVIDVTSYPNVTDLFLAADMMITDYSSVM
                     FDYSVTGRPMIFFVPDLEQYRDQTRGVYFDLEETAPGPVLFTQDAVVAAIRNAESDAP
                     GYAEKYVAWREKFNAHDDGHSAERVIERLFALPKK"
     gene            complement(15972..17309)
                     /locus_tag="PKJ61_00650"
     CDS             complement(15972..17309)
                     /locus_tag="PKJ61_00650"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOC12373.1"
                     /translation="MRVITYGTFDLFHEGHRRLLERAKALGDYLIVGVTTENYDDSRG
                     KLNVRQSLMDRIRNVQDSGLADEIIIEEYEGQKVQDIQKHGVDIFAIGSDWLGKFDYL
                     GDYCKVVYLDRTKGVSSTQLRNESAGVIRLGVVGSGRIAARFVAEARYVSGVSVEAVY
                     SRRFESAEAFAEKLELEWANETYADLLKHVDAVYVATPHATHYDYARQAIEAGVHVLC
                     EKPMTLTKAATEELFELAAQHEVVLLEAVKTAFAPGFQRMVAVARSGSIGQVRSVDAT
                     FTKLVKSGRELEGEDGGSISELATYPLLAVVKLLGTDYTKVTTHSLKPAGSPVEVFSR
                     IDVDFPGGIASARTGIGVKAEGELIVAGSNGYVYVPAPWWLTEYFETRFEDQSRNKKY
                     YYKFDGDGLRYELAEFAFMIRNGTPESFKLRAAESIAIADIIEQARSQAETFE"
     gene            17397..19052
                     /locus_tag="PKJ61_00655"
     CDS             17397..19052
                     /locus_tag="PKJ61_00655"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOC12374.1"
                     /translation="MKLPGPLNRITGRGADAAPATGRTDRRPDARPLDPAPSLQPFPA
                     ARYLYAVNAIPRNYAGRSSSIITKARVFAEQAGVRSTLVTFLHSAELADIEHDLRERG
                     VLGDHLDFVCLHDYYPDESSYTGEPVEHPIDDPELNWLKEPTQPVYRYFDSTGTYRRY
                     RRYDHAGRLIVQDHFNPNRGRTLREEFRPNGTLRRRVYMDLAHNLPRQEIHYRADQTP
                     AFTVWWVVDADAQESVVEKVITFDEAGRPTGMADSMDAINHLCLDRLIGDEQAFVMVE
                     DRHVDRYLLSYQRPHVRMLYVLHNAHIKEPYDDPNAIRPSFRPILTARHRVDAIVFLT
                     ATQRAEAEAKYGRADSFRVLPHSVAAPELTPGVAREPNLVVMMARLESKQKQVNHAIE
                     AFAQVVAQLPQARLEIYGRGPEQAALQTLINDLGVAKQVKLMGFTKEPHLVYQRGALC
                     ILTSRFEGAPLTVQEAMSYGCPVVSYDLRYGPADVITDHVDGLLVPYGDRKAMAATVV
                     AALRDPELLARMSAAAPKRAAEFGEAPFAARWSALFNEFAPKG"
     gene            complement(19057..19740)
                     /locus_tag="PKJ61_00660"
     CDS             complement(19057..19740)
                     /locus_tag="PKJ61_00660"
                     /inference="COORDINATES: protein motif:HMM:NF016149.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopC"
                     /protein_id="HOC12375.1"
                     /translation="MKSARWLAVMVSALLMVGFIGHGSAWAADDFLDSSDPAPQQELK
                     RVPGWVTLVFKADSDPSLATIVVLDESGANVTTGPLIVEGTNVTTQLRSKLPKGTYTV
                     IYRTVDEFGDKRGGSFQFAYGKGSWTPLESEVWQGEDQQPTILDDVEDPSATPAPTPT
                     PSETPTPTPIATPSPAATPTVSASPAPVGPVDLGDSTAWILGGGAVALALAGGAGLLA
                     WRRRQREQG"
     gene            19935..21350
                     /locus_tag="PKJ61_00665"
     CDS             19935..21350
                     /locus_tag="PKJ61_00665"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HOC12376.1"
                     /translation="MFGAVSLVAALATTPAAAIDWTINPDTQEAAAATNASADASSCP
                     TLTTVPGGVEAWFNTDDMEYRGLGDTNNAPSATWSFAHKVAQIICGAKQGANIYVGMY
                     FIRAIGTAERPETDTDVIWNAMKYVIANRGVKIGFVMDGGGITSSSSKAQIKKKLAEI
                     GAGIYWCYNGCLNTNTAKKFPYAINHEKFLAISDTIWAGDSAQHPAVYSSSGNFARSQ
                     VRNYWQEATLVYDDVRLYNFTVDRYNAMKVCTGSSTGGSNCKSGKFPGAKQSGIVTAA
                     GSLKKLRGIWTDSYFRRYTDAGRGTTISFSPQPETVLDYYVEQFNDVDCTVDNKIRVA
                     MYRLTDTRAVRFIDTVKTLKRQGCDVRILLSQTGGAQTISKQVSKQIKAAGLTEQINC
                     TRIPIHTKMILIGPATNNNGRILFGTANMSTSAIRYSEEHVITMDSRRADDAMAADIR
                     QVYGVYQAGWNELNQGSRTCK"
     gene            21347..22879
                     /locus_tag="PKJ61_00670"
     CDS             21347..22879
                     /locus_tag="PKJ61_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HOC12377.1"
                     /translation="MNTNRTHRHSPWFALLAAFVVVLGLLVPAANADAAKYPYSAPPE
                     LSAVNSAKTSVELFWRTVTGAPGYRVRAVGGGQTLYQNTGSDGTTVFKGLAPSTSYTF
                     NVAVLQVTDGKQLSAWSKAKATRATTAATMLEPPTDLKMTKQTPNTLTLSWTPPAGFD
                     PAAHAIRVDYAEDQAMTTGNGSQFFSGNSGTLNGLSTNTNFFVRATVVGITDHAQVGD
                     RSTAVLAKTLSPIGWIHGTVTGAPDAAFANYSVAAYSVASGDVNQQVNLTKKGSSWEY
                     TLQVRPGGYYVQVVNVGPANYTSLWAKSGADGALIQSQGTQVSVAMGGTTEAPAVTVG
                     KGTVLKGQIDCPGQGTKDSCSIDVSALLDGQVVAANRSDSSGKYEIAGLPAATYTVRT
                     SHAEDRFVAAKETVAVAGDSVTYNHTMAKRDFLTTYKVKFVKKGKVLKWSSRAYLAAV
                     LPTERADNNDFQWYRNGSPISGATGSSWRISGGGKIKLCVRYGRFGFHTASWACSSAK
                     KV"
     gene            22898..24571
                     /locus_tag="PKJ61_00675"
     CDS             22898..24571
                     /locus_tag="PKJ61_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC12378.1"
                     /translation="MLKHPRRWVRRVLGRSKTDAQPAVLRLEFSTPEQFAAFVSDPRA
                     RRKYRALELRVSPWYAIRPDWTGRLGPLKGVRSLRTDFDQAGRVASAGLVLAEPIAGY
                     ELVRAAFGLFYPVRPTTGWGGRRVAVDAGAPVADCWQPGGGLRPLNPRKQKKQFTDFE
                     VLVDGEGEFWLGEHPAPPVLIERTPGSQILIDPKVHRPLERRQPSPSTVSATARVRDG
                     RLLIDSNGSLLVDADASAGLTATNLRQLGLVTDIDASGVGGGLVAGRLVAQLAAYGAV
                     VHDAAPGLELSPELARLVESPFARMGLLDHMNRSLAQVRAVMTGHTRAFTEGELPSVS
                     VILSCQRPNLLVRILEQLAAQDHPNVEVVVGCHGFPAPARESFPEPVLGVLGPVLEFG
                     SDMLFGDVLAGLSAAASGDFITKVDDDDWYGPHHLTDLLAAWTYSEAHLVGKKLALVH
                     FEEDDSLVIRRFFLEGYRWQVAGGAAIIAKHDLTAVGGWRSQRRAVDRGLWTRMEEAG
                     ALTYACSGPGYVHTRHAGPHTWAVNDQHFRESYAEETLTGIPPAALGVR"
     gene            complement(24575..26479)
                     /locus_tag="PKJ61_00680"
     CDS             complement(24575..26479)
                     /locus_tag="PKJ61_00680"
                     /inference="COORDINATES: protein motif:HMM:NF016357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HOC12379.1"
                     /translation="MSDEEQVESESEDPKAADAAQVVRMPQSFEFARAVLGTPQMPAW
                     LGWLDRLLSGYYLAVTGAIWGIVVACIIGGWVVAGIIFLALEVLTDWLASRSCVRETL
                     LMRRLGVGANVRQLIRGVLLVALVGRGGDGWLAAAVAAAVLLIQMVHLTLRAGSAWLS
                     GRQPALAYRTNGEPQPAASVAFAREYRASAWQNPLVVVLELLVASWTLVAHVLGWQLW
                     LNVGFVLVLVVVLGVLGVRHGQRLRVLGSDASVARVLNQVRAELDAIAPEAVVYMSAD
                     AGQSLYILNQWVPAIEALHQKSFIMVREASHLQPIAPTPVPVVYAPKTRHVEELIRPT
                     TMVAYYLANAGKNVHLLREASIRHVFLNHGDSDKSTSANPVSRVYDGVWVAGQAAIDR
                     YEAAGIHMPDSQYAIIGRPQVEQLRVGPIAEDHRRTILYAPTFEGYYEESNYSSLERM
                     GPQMIRRILAELPDVRIIFKPHPASGVQRPGMAQARVEIVELLAAANAGHLYVGPEAS
                     ITLYECFDAADALISDISSVVTDYLYTERPLITSNPRELDLATYYNTFPTQRASYILE
                     HDLSNLSQILADALADDSLRADRLKMKKYVLGDLPAGPLAAFVAEGDRITAEAKLHAA
                     SIQNEFRVKS"
     gene            complement(26552..27346)
                     /locus_tag="PKJ61_00685"
     CDS             complement(26552..27346)
                     /locus_tag="PKJ61_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020576876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOC12380.1"
                     /translation="MTEPLTPLRPVITPDSVAADGRRPVVIVDDLHVKYRVYSSGKAI
                     KRNTRGVLKAPSRGIRVVHALKGVSFVAYENESIGVIGSNGSGKSTLMRAVTGLTPPE
                     SGAVYAASRPNLLGVGAALIPDLSGDRNIMLGGLALGLTRKEVEELHDPIVNFAELSE
                     FIDLPMRTYSSGMQARLKFAIATIKHHEILIVDEALSVGDRRFRQRSENRIREIRDNA
                     GTVFLVSHSMTSIRDTCSRTIWLNKGDLIMDGPTDEVVTAYEESRR"
     gene            complement(27339..28187)
                     /locus_tag="PKJ61_00690"
     CDS             complement(27339..28187)
                     /locus_tag="PKJ61_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007076965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOC12381.1"
                     /translation="MSLAALAEDHGLHRVGARPPFFAYLKQAWQRRDFITAMAGYRLR
                     ADLEGNRLGILWLLLQPVLNAVIYGIIFGVLQSSATRGEDYAARVVIGVFLWQFFAKS
                     LTQGAKSITGNQSLVQSLAFPRLTLPVAEVIEQFLSLLPSMGLLVVLLPFMGHPPTWS
                     WLGMIPLLFLFTLFNTGVALIAARLTVHVQDLSQLLPYLSRILFYTSGVLFDVSKIFK
                     AHPAVMQLYDFYPLYQVLQMARSSLMGGFSYDPMYWLYFSIWSVVTFVVGLLFFWAAE
                     ERYGRD"
     gene            complement(28409..30085)
                     /gene="pgi"
                     /locus_tag="PKJ61_00695"
     CDS             complement(28409..30085)
                     /gene="pgi"
                     /locus_tag="PKJ61_00695"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015069394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HOC12382.1"
                     /translation="MSAPVDATTTAAWAKLTELAADLKPDLPGWFAEDPGRAARFSLT
                     AADLFVDLSKNLITDEIRSALVELGQEVGLETKRDAMFAGERINVTENRSVLHTALRR
                     PRTDSLVVDGTDVVAQVHEVLDQVYAFADKVRTGEWRGVTGSQIKTVVNIGIGGSDLG
                     PVMVYEALQPYVQGGLECRFVSNIDPSDVAEKVKDLNPATTLFIVASKTFTTLETLTN
                     ARMARDWFLAKLLEAGAVDGSDEQNRNAISKHFVAVSTALDKVAAFGIDPKNAFGFWD
                     WVGGRYSVDSAVGTALVVAIGKENFADFLAGFNAIDRHFVETPLASNVPALMGLLNVW
                     YVNFIKAGSHAVLPYAQYLHRFAAYLQQLTMESNGKGVRFDGSAVTTETGEIFWGEPG
                     TNGQHAFYQLIHQGTQLIPADFIAVATPAHPTKDGDADVHELFLANFFAQTKALAFGK
                     TADEVRAEGTAEAIVPARVFPGNRPTTSIMAPALTPSVVGQLIALYEHITFVQGVVWG
                     INSFDQWGVELGKQLALQIAPAVAGDADALAAQDPSTQNLVGYYLAHRAR"
     gene            complement(30142..32004)
                     /locus_tag="PKJ61_00700"
     CDS             complement(30142..32004)
                     /locus_tag="PKJ61_00700"
                     /inference="COORDINATES: protein motif:HMM:NF016357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HOC12383.1"
                     /translation="MNIGGVLGNLGRAPVPETEEVARAAWPGRVPVRVSVMAAQAFLP
                     VVALCTIVLLLALWLRLPWLGLVLWTLGALVEWLASGTDSPVVRLLDLVGLRAPLRAL
                     LRSLPAAGLLFLTTPNGVVAGSAYVTVVLLAQLAWAVQPVLATWVSRSAPPLRYSAER
                     KRTRPLSVQAEQGGDSSFSVHEADAFTAHARVYARAVGTPFALAAIEFVALAKAVALG
                     YGMMPVWLGLSIAGALALVAIGYLLWTGLAARSVRAQQPGWGEQVLAELGAIDPRFVV
                     YVSLAARQSKYIVNQWLPALDALAEPGFLLVREASQLLPIGATRLPVIYAPSQRDVER
                     VTLAGVRAAFYLAYGERNGQLLREPRLKHVMLLHGDSDKATSANGMARAFDEIWVAGP
                     MAVERYRAAGVDLPAERFRFIGRPQAAGLPSGPTGHETPVVLYAPTFEGYYDQTAHSS
                     LDRMGVELIRRLVARGDVRVWFRPHPSSGVSRPSMLAAIDQINTLLRSAPGGHRVVAD
                     QSLTLPECLAEADLLITDVSSVATEFLYTGRPILTCDPAGLGGSAFAATYPTSVGSYV
                     LEPDLADLDGLLADAFGADPKRPARAELKARVLGDPPGGPQAAFAANVARVCAD"
     gene            complement(32027..32377)
                     /locus_tag="PKJ61_00705"
     CDS             complement(32027..32377)
                     /locus_tag="PKJ61_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC12384.1"
                     /translation="MRAFLVMVLTVACMVGLSTLVTPALAAVPTGVAAMPQPEATKTK
                     KAKKKPKATPTPTPVATATPSPSPRVRTAAELEGDRWVQIALVAGGGLLGSVLAFFGI
                     GALMRSGRRRRAGN"
     gene            32547..33512
                     /locus_tag="PKJ61_00710"
     CDS             32547..33512
                     /locus_tag="PKJ61_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC12385.1"
                     /translation="MYQPRRIADPIEPALGVPASGAANFDSPTGGPRRALPASTDHWY
                     RRGSVALAVVTTAATAGAVILSNTSTTAAALGGPVLVEQPVLAAAIPAAPMAKLVAEE
                     LAAPKLVGSAKQADTAMLSLTKLYSSGSLNVRAKANSKSKLLGKVTTAATVKATTEID
                     GDYRKVEFEDGYGWVLADELTDNSPDGVFIDDGTVPEGTTMSACSRGSGVEGKLRKDT
                     VHIYRSVCALFPAVNSYGGWRAGGRQFHKNGRALDIMLTPQKESALGKQIAAYLIKHA
                     KDFNIDHIIFEQKIWTPSTPRWRHMADRGGITANHYDHVHVAIRG"
     gene            complement(33564..34169)
                     /locus_tag="PKJ61_00715"
     CDS             complement(33564..34169)
                     /locus_tag="PKJ61_00715"
                     /inference="COORDINATES: protein motif:HMM:NF013263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC12386.1"
                     /translation="MASKMPGLGAHSLIVSLPDFDLPDLVPAAEVLWQEGFRVWSVVF
                     ERLDDFPELQRRFGRRAKLGVHGVSEPEQLRTAAAAGAAFVAAPFLRPQLVDAVPGLP
                     VILGGMTPSELLAGLDAGAAAVQLLPTDAFGTGFARSLPPMLPPGTLIANGRMERYQA
                     DIWMDAGALGVWPQDLLSPELVLAESLDGLRSKLQKWRLGD"
     gene            34267..34734
                     /locus_tag="PKJ61_00720"
     CDS             34267..34734
                     /locus_tag="PKJ61_00720"
                     /inference="COORDINATES: protein motif:HMM:NF013340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HOC12387.1"
                     /translation="MEFTSENIAAGSSIDLRYAEPGVGGDNVSPQLTWSAGPDGTQSY
                     ALTCFDPDAPTGSGWWHWVVTDIPAEVTSLAEGASLPAGARTWTNDYGYPGWGGPWPP
                     PGPEHHYVFTIFAVGVPRLDVPDDASSAVARLTLSFNVLESGSFTAVFANPAS"
     gene            34747..35640
                     /locus_tag="PKJ61_00725"
     CDS             34747..35640
                     /locus_tag="PKJ61_00725"
                     /inference="COORDINATES: protein motif:HMM:NF024621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4031 domain-containing protein"
                     /protein_id="HOC12388.1"
                     /translation="MAVLIDPPRWPAHGTRFAHLVSDATLDELFEFVDAAQVPPRAFD
                     HDHYDVPERRYADLVSSGAEPVDEKTLLRRLKDSGLRVRPRDRTPRTAQVLPALERAW
                     TELLPEQVSLGAELLERWQEPNRHYHDVRHLGQALSALAELDSRQSRTARLALWFHDA
                     VYTGSAGADEDASAELAVSSLAKVGLPTATVDEVARLVRLTSTHTTDDGDDRGALVLD
                     ADLSVLGTAPGRYHVYSRDVRLEYPQFSDAEFASGRLRVLDRLLNLDTLYRTPVAQQR
                     WLASARSNLASEHRRWARLAA"
     gene            35924..36769
                     /locus_tag="PKJ61_00730"
     CDS             35924..36769
                     /locus_tag="PKJ61_00730"
                     /inference="COORDINATES: protein motif:HMM:NF014600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HOC12389.1"
                     /translation="MSGVSKALLIGAVASALALGGCAVAPPEASLAPSSTAASSTNPP
                     AATPGTPTSAPSATSATPSESASPATAAAKPSAAAKPSARAKPAPAKPAPDQRKGKQL
                     NTPYEVDGIVVVSRQHRVSAAYKPKNPTGPYGLTKETAAALKKMTKAAKADGVRIVVR
                     SGYRSYATQKGVYQRALKNYPSVASAKRYNAPPGASEHQTGLSVDLWDGVTWSLPMAN
                     TATGKWLHKHVHEYGFVLRYPNGKEKITGYAYEPWHFRYIGVADAKKFGKGNTLTLEE
                     YLGLA"
     gene            36833..39082
                     /locus_tag="PKJ61_00735"
     CDS             36833..39082
                     /locus_tag="PKJ61_00735"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002534437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOC12390.1"
                     /translation="MTANLPDTAGTEKIGAGKRIGILTSGGDAQGMNAAVRAVVRTAK
                     TLGARVYAIYEGYQGMVDGGTGISELAWEDVGNILGKGGTIIGTFRSKDFREYSGRLR
                     AAANLLEHGIDRLIVIGGDGSLSGLDQFRAEWTQLLADLVASGEITKETADAHPSLMF
                     AGLVGSIDNDLVGTDMTIGADTALHRIVDAIDALSSTAASHQRSFVVEVMGRHCGYLG
                     LMSAIAGGCDYVLIPEQPPEDGWEERMCHSLRRGRDGGRRDSMVVVAEGACDRHGQPI
                     TSEYVRKVIEEHLQEDARVTILGHVQRGGAPSAYDRWASTWLGYEAVREVLSAEPGTT
                     GPVIGFRGNRVTRVPLVEAVANTRKVPELITSGDYAGAMAGRGGSFTEAAQIFAELVE
                     PSRVDAPEESKRIAIIHAGGLAPGMNAAARDAVRLGISRGHTMLGVRNGFPGLRDGDI
                     GEIEWGDVENWLTDGGAELGLRRTVPGLDELYRISRSIEDHRIDALLLIGGWNGYEAA
                     ELMHRERDRFPACGLPIVCVPASIDNNLPGSELSIGADTALNVITEAIDRIKMSGSAA
                     KRAFVVETMGRWCGYLALMGGLSGGAERVYLHEEGISLDDLSSDLDWLRNSFASGRKL
                     FLAVRNENANALYTTDFLARLLEQEGRGLYDVRQNVLGHIQQGGCPSPFDRLLAVRLV
                     SRALDVISAEFTAGTRNSWYLGLVQSKVTSFPMTHMMDEMDKRYNRPKEQWWLALRPV
                     LKAVADAGT"
     gene            complement(39117..40169)
                     /locus_tag="PKJ61_00740"
     CDS             complement(39117..40169)
                     /locus_tag="PKJ61_00740"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008355368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HOC12391.1"
                     /translation="MPEATAYAIDGPTGRFARTTITRREPGPTEVLFDIVYAGICHSD
                     IHTARGEWGRVNYPLVPGHELAGVVREVGSEVTKFAPGDRVGVGCFVDSCGECEQCRA
                     GDEQFCDGPGGAIWTYNGTGRDGLPTAGGYSTCITVEQDYICRIPDSIGLQQAAPLLC
                     AGITLYSPLKRWGAGPGKRVAIVGMGGLGHMGVQIAAAMGAEVAVISQTRSKESDGRR
                     FGATEYYAVSEPGTLRSLRKQFDLIVSTVSADQDLNGLINCLKTGGALVDVGLPEHPV
                     ELNLGGLVGQRRVLAGSQIGGIAETQEMLDFCAEHGLAAQVEVITGADINAAYDRVVA
                     SDVRYRFVIDTATFAG"
     gene            complement(40328..40693)
                     /locus_tag="PKJ61_00745"
     CDS             complement(40328..40693)
                     /locus_tag="PKJ61_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002533767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HOC12392.1"
                     /translation="MIPLLTALAGGLGAVVRFLVDRQIAARLRVGMPLGTVLINVTGS
                     LLLGVITGWWMSHSGDPGLRAVLGTGFLGGYTTFSTASVEAARLALDGRGWTTLVHAG
                     GMLVLSLLTALLGVYLGSL"
     gene            complement(40690..41163)
                     /locus_tag="PKJ61_00750"
     CDS             complement(40690..41163)
                     /locus_tag="PKJ61_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015069383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HOC12393.1"
                     /translation="MTPSSSRPHTPSSSRRRPGSPDWRWLLLILLGGTLGTAVRAWLE
                     STWPAQPGAWPWTTFWINIGGSLMLGLLLEGLAISGSDAGWRRGVRLGVGTGVMGGFT
                     TYSTFAVESVERLGGGQWLVGAAYALVSVVFGILTAIAGLRLGRTVFSRLLGEAA"
     gene            complement(41160..41720)
                     /locus_tag="PKJ61_00755"
     CDS             complement(41160..41720)
                     /locus_tag="PKJ61_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015069382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOC12394.1"
                     /translation="MSTKPPGRPFTESAPQPAVDRIVPATAHALVVGVVPGQSPIVAV
                     TAATWATAVGGTLHFAYVDPERVLDKEYADGSVRHSALSPDSDDDSWLERQEQLRAQI
                     TSALTGHEVHWELHYLAGRADRAVTHLARALDASAIVVGARRPVPGERLREFLAASLG
                     AQLTRHQHRPVLAVPVEVVDWKHQVQ"
     regulatory      complement(41753..41823)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(41905..43215)
                     /locus_tag="PKJ61_00760"
     CDS             complement(41905..43215)
                     /locus_tag="PKJ61_00760"
                     /inference="COORDINATES: protein motif:HMM:NF012475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HOC12395.1"
                     /translation="MTPPAGFLGDPNGLIHWQGSHHVFYQWNPVATAFGRMHWGHAVS
                     SDLLRWEHRPVALVPGATDALDGTGCWSGGAALTAHGEVELFYTAVSDGAQKQRPRVA
                     RTTDPGLDSLVPGALVATPPDPAIHDFRDPFVWWHEGQRHLILAAVLPDGQPSLLVYT
                     ERESSPGADWEYAFALGRDALPGVPGRLWECPSVIKLDGRWVAMLSVIEGEGAAERLS
                     VWAVTLDREPGQQARPLWVGRLDAGPRYYAALPWQHADGRWLQLGWLRTQDDPDRGVS
                     TWVGAMSCPRELTLVGDRVVLRPAAELDDLVRTPLGRGHLGSDRAIALGDPGLRRDDD
                     GLRAGNSPTQLEIVAITPSTGTLILEGPEAELMIPIGELVEPESSGEVRIFIDAGLIE
                     AFGPLGARSDTSSRIADLVSVSPSDGAGISEVNRLSLADNLPSR"
     gene            complement(43365..43526)
                     /locus_tag="PKJ61_00765"
     CDS             complement(43365..43526)
                     /locus_tag="PKJ61_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HOC12396.1"
                     /translation="MTPGQDTCAVCGMPIEDGVDFTSVNGDQTYRFCSQACQDTLADN
                     PGPYANSQS"
     gene            43654..>43842
                     /locus_tag="PKJ61_00770"
     CDS             43654..>43842
                     /locus_tag="PKJ61_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC12397.1"
                     /translation="MSDSVNDYGLGAPNTQQDATPEPTRQYEEPNPLVHGLGDQDLPD
                     DDHSEAGRRHRSHQEHGLG"
     assembly_gap    43843..43942
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(44202..45971)
                     /gene="treZ"
                     /locus_tag="PKJ61_00775"
     CDS             complement(44202..45971)
                     /gene="treZ"
                     /locus_tag="PKJ61_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HOC12398.1"
                     /translation="MPEVRRPGERGRFDVWAPRAERVRLLAGERTVAMAKAADGWWHP
                     VEPEPLGRIDYGYLVDDRKAALPDPRSRWQPHGVHGLSRTFDATGFTWHDQAWTGRQL
                     AGGVIYELHVGTFTPEGTLDAAISRLPHLVELGIDFVELMPVNAFNGEHGWGYDGVCW
                     FAVHEPYGGPDAYRRFVDAAHGLGLAVIQDLVYNHFGPSGNYLPAYGPYLTDQDSPWG
                     SVVNLDGEGSAEVRNYILDNARYWFTDMHVDALRLDAVHALVDSSPVHLLEDLAVVTA
                     AASAHLRRPLTLIAESDQNDPGLVTPRQSGGLGIDAQWSDDFHHGLHVALTGEAEGYY
                     AEFTSLGALAAACERGFVRDRVAVPGGGSEGPPQLDARTVPGWRLVICYSNHDQIGNR
                     AAGDRLAASLDDDQLACAALLTLTTANTPMLFQGEEWAASSPFPFFSSHPEPELAEAI
                     RSGRMAELARMNWDLETMLNPQEPQTFAAAKLAWSELADGRHALLLDVHRDLIALRRE
                     LPELSDPAMTGTHCAYDDVQRWFVMRRGGVVVAVNFGSEEVSIELSGRHQLRWASPSG
                     ARVADGGVVLPPHAGAVLLPVQS"
     assembly_gap    45993..46092
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<46093..48203)
                     /gene="treY"
                     /locus_tag="PKJ61_00780"
     CDS             complement(<46093..48203)
                     /gene="treY"
                     /locus_tag="PKJ61_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017933055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HOC12399.1"
                     /translation="MRPVPASTYRLQITEDFDLVAAARTLSYLHELGVDWVYLSPLLA
                     SESGSEHGYDVADHRAIDPARGRSSGLAALASEAKRLGMGVLVDIVPNHVGIAQPWEN
                     EWWWQVLTNGPDSPYAGAFDIDWAAGGGRLRLPVVGDDDLCADGRIDHLQVLGGELHY
                     HDQRFPLAPGTAHGADEDPNAVHARQHYELVSWREADRSLNYRRFFAVNTLVAIRVED
                     PEWLHRSHAEIQRWFDEGLVDGLRVDHPDGLRDPGKYLDDLAALTGGAYVLVEKILGL
                     HAAGEGGSVRLEELPPTWATAGTTGYDAMAIIDRVLIDPAGEVQLTTLENRLRGRDVD
                     WAELVHQGKLAVAEGILNSEVRRIAREVLAVIDPSGWQPDPDALADAVAELLADFGVY
                     RSYLPEGRDHLERAFEYARLHRPDLGPVFALLHPVLADGASGPAQRFQQTSGMVMAKG
                     VEDGAFYRWSRLTSLNEVGGDPSIFSIGTDDFHLLMNQRQAVWPNAMTAGTTHDTKRG
                     EDVRARISVLSEVPDLWADALSQLLELVPLPDPGFGNLLWQAIVGAWPAERERLIGYA
                     EKAMREAGDHTSWTAPDHAYEAVVLEAVDATRDSAKVRTVLQGVVDAISAAGWSNALS
                     AKLLDLTVPGVPDLYQGSELWELSLVDPDNRRPVDFAARVALLAKVRTGARPPLPSGL
                     DGAAVKLLLCHQALTLRRERP"
     gene            complement(48334..48708)
                     /locus_tag="PKJ61_00785"
     CDS             complement(48334..48708)
                     /locus_tag="PKJ61_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC12400.1"
                     /translation="MSQIRLTSRPGPPRPLFVKDTDSLGEQLQSAFPETRVVKTLNTL
                     NADLMAHPEELADGEPTVFVAGNDADAKRVVIALLAQLRHRDVIDLGDITAARGVEMW
                     LPLWLRLMGALGTAKFSLRIVR"
     assembly_gap    48850..48949
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<48950..49534)
                     /locus_tag="PKJ61_00790"
     CDS             complement(<48950..49534)
                     /locus_tag="PKJ61_00790"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOC12401.1"
                     /translation="MSESEPIGAAHARVLVGVDGSADGLRAVKYAMREANASGSDLWL
                     VNVVDDQAMVTGLWDLLSTIDVLRQAGADKLKVAADQLEADGFPSDRVISEVLIGRPG
                     DVLADLSDKARLLVVGRRSISGLERMFVGSTSVSVVGRANCPVIVISAAATPQQTGGL
                     GTIALAVSSWPLHRSALDWALQEAALRNARLRVVH"
     gene            49649..50401
                     /locus_tag="PKJ61_00795"
     CDS             49649..50401
                     /locus_tag="PKJ61_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HOC12402.1"
                     /translation="MSEKQSREAYILDLLAKDGSLSVAALSRDLGVSEVTIRTNLRDL
                     EARGLLLRTHGGAQASSFQTVLERQRHHMAEKERIAAVAASLVEDGDSLMVEAGTTTA
                     LLLKSLLGRQGLQVVTNSTLTIPAARLNPGLRLIVTGGLFHHETESFTGSGALATIRD
                     YNVRLAFIGTDGFTAERGLTTQFAEGADAIRAMHSRAEETWLLADSSKYGRAGFVNVL
                     PLHDVAGVITDAGIGEESVSALRELGVRVLIA"
     gene            50702..51469
                     /locus_tag="PKJ61_00800"
     CDS             50702..51469
                     /locus_tag="PKJ61_00800"
                     /inference="COORDINATES: protein motif:HMM:NF004203.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOC12403.1"
                     /translation="MDFTGKVALVTGAGSGIGEACAVRLAELGAEVAVVGHSLDSAAA
                     VVEQIRTRGGAAEPFAAEVSDADAVERLFEQVGRRWNKLHVVVANAGINGVWAPLEEL
                     SVEEWSRTIEVNLTGTFLTVKYALPLLKVVVTASVNGTRMFSNSGASAYATSKAGQVA
                     FAKMAAVELARHRVRVNVICPGAIETEIDDNTEQRNTDGLHVPVEYPHGQIPLTGSRP
                     GSAAQVAELICFLGSDAADHVTGTEVWIDGAQSLLAG"
     gene            complement(51603..52295)
                     /locus_tag="PKJ61_00805"
     CDS             complement(51603..52295)
                     /locus_tag="PKJ61_00805"
                     /inference="COORDINATES: protein motif:HMM:NF023697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3618 domain-containing protein"
                     /protein_id="HOC12404.1"
                     /translation="MTSNPSEIRAQIEHTRSSLSDNVDALADQANPAHIAHRQVSKVK
                     AAGGRFLDRLLGTAEDVKDAALGGVQHAVEGVHDAASGVAGRASEVGDSVAGVPRAAR
                     QQTQGNPLAAGVVAFGIGLLIAAAFPPSRKEKELAQAVKEQAQPLTDQLAAAGQEVAQ
                     NLSEPVQQAVDSLKESATGAVQHVQEQGASAVAEVKEVAGEAASDVAGTAKSAADDVK
                     REARSAVEGNAG"
     gene            complement(52292..52732)
                     /locus_tag="PKJ61_00810"
     CDS             complement(52292..52732)
                     /locus_tag="PKJ61_00810"
                     /inference="COORDINATES: protein motif:HMM:NF018980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HOC12405.1"
                     /translation="MSGPLDPVGQPAAVPAVVPEERSIGELLGDVSRDLSTLMRQEVE
                     LAKAELRESASQAGRGAGLLTGAGVGAFLSVLFLSIALWWGLGYLIGNAWSAVVVAVV
                     WGIVAAILAASGRTELTRVRGLPKTAETVSKIPNALKGNEEDNR"
     gene            complement(52729..53427)
                     /locus_tag="PKJ61_00815"
     CDS             complement(52729..53427)
                     /locus_tag="PKJ61_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC12406.1"
                     /translation="MKLNEGIQTGSVGVPDYNRQPGVAESAQHLAETGAEQAGVVKDT
                     VLADAAEVAGVAKDELAKLASDARTQVQGLWSQASGQLREQAGNGKQQLGDLLHSLAA
                     ELGQMASKSDDGGPLTALAKQAAARGGELSHWLQESEPADVLVEIKRFARRRPFVFLA
                     GAAVAGMVVGRLSRGLMAADESRPGQPSVARASGYSELGSSAGVRAVPTTTRVTEPQL
                     AVEPDLGAPGGVLR"
     gene            53609..>53944
                     /locus_tag="PKJ61_00820"
     CDS             53609..>53944
                     /locus_tag="PKJ61_00820"
                     /EC_number="5.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase family protein"
                     /protein_id="HOC12407.1"
                     /translation="MALTQIVLIRHGESLGNVAAAAANAAGLEAIEAGARDPDVALSP
                     AGLEQATALRRGLSLLLSDGVSTAIWSSPYRRAVQTAEVALAAGVARPAIRIDERLRD
                     RELGVLDLLT"
BASE COUNT         9121 a        18657 c        17590 g         8176 t
ORIGIN      
        1 cgctttccca ttggagcgga tgacgggaat cgaacccgca ctgtcagctt gggaagctga
       61 tgttctgcca ttgaactaca tccgcgcgca ccggctcacg gcgcagccag cagtctagcc
      121 gcacctgggc ggcgcccgca caacccccgg ccctgcccaa caacggacgc cgcccagcag
      181 ccagtcccgg gacaattcgc gtccgatcga gcgcgacacg cgcggtggcg caggatcgtc
      241 acccattcga cgtcatcggt cccatcaggc gacgcttcca ccgcgtacgt ctcgagtccg
      301 ctgagcatgc cggttcgcct ccgcggtgat tcgactcgtc ggcgctccag ctttcgcctc
      361 gaatggggag ggaaagccca cgacccacct gaacctcagg cctcggagcc agaccaccaa
      421 accaccccgc gcaccccgca caccgagcgg ccggagccct ttcagcgccc gccgacgagc
      481 ggcgtccgtc cgacctcggg cgattcccta cttatccaga cccttcctct gccagactgc
      541 tttcgacagg atcatcgttt ccgggttgca gaaagtgggc gagtgagcaa ggaagagcgg
      601 gccgtcacta ttgtcgaact ggttacccag acaatgtcgg tgctcaacga cggcgaaaag
      661 ggcgagctgg cggtttgcca cggcctggcc aaggccaccg cgtcggacgc agccgtactc
      721 atcggaaccg acttcacccg ccgggtgcga gtgcacgcgg tttggcccaa tcatcgcgct
      781 gccgcccctc tggccgaact cgtcgtggcc gcgcacggcc atccggccgg cgaccatccg
      841 gtgctcggcg aggtgctcac cttgaccttc ggggcaccga acgagtccca tccgcattcg
      901 aatgcccgca caatcgtgct ctcccgccgc gctcgcttca cccccgaggc ccgcatgttc
      961 ctcaccgagg ccggcccggc gctgcagttg ctgctccccc acgtcgggga cgcgtgcctc
     1021 gcggctgaac agtcccgcgc agcagcggac gccgccgcag ccctcaacct cacctctcgt
     1081 gaggtcgagg tgctccaact cctctccgaa ggactactcg cccgcaccat cgcggcccgc
     1141 ctcggactct cgccgcgaac cgtgcacaag cacctcagca acgtctacac caagctcggc
     1201 gtgcacgatc gcctggtcgc ggtcagcttc gctcgtgatc gcggactgat cagcgcctag
     1261 ccccgacggc cccgtccggc caaccgagca ctacttgcgg cgtgccttcg cctgcgcgac
     1321 cttgtccttg agcagccaca gcggcagcag ccagccaccg atcgccgtca ccagccacac
     1381 caccagggtc gcgcccagcc agggcagcgc gccgccgtcg aaggtgattc cgccctcgaa
     1441 caggctggcc accagcagcg ccagcccggt ggagatcagt cccatgccgc cgatcagcgc
     1501 ctgaccagcc tttcgcgaca gccgcatcgc cagcggcgtg atcaggcttt gggcgagggt
     1561 gaacaccagc accgccacca ggaaaccacc cggccgcagc accacaccgg ggatcaacgc
     1621 ggccagcagc aggccgacca ctgccagccc gaagtagatg ccgccccgaa tcagcattcg
     1681 aatcatgccg gggaatctac tcccctttca gcgtttcgca gtcagcctcg ccagcacctg
     1741 ggccaccgcc ttgctcgcag cgctgacccg gacgctcagc gcggacactt cgtccttggt
     1801 gagcgcgtcg accgggacat agcgcgcatc gccggtgaga tcggacgcca gcacctgcag
     1861 atcaccctgg acggccttgg cagccttcgc cgcagcgacc gcgtccgggc cggttgccga
     1921 cgccggcagc tcggccaccg cagcctcagc agcgttcacc cttccgggca cggtgcagcc
     1981 cttcggctca tgcaggccgg acccagccgc ggcagcctcg gcctgagccg cccgaaccgg
     2041 ccgcagcagg ttcacctgcc cgttcaccac gaagggcgca gccagaccag cccccaccaa
     2101 ctgcgcgttc agcaactccc cccgctcgct gtacaggccg accagcgtgc ggccgaagcg
     2161 atcgtgaccg tagttctcga tccgcaccgt ggctccattc ggcgcgagtt cggccagctt
     2221 ggctttcgcc gccttcgcca ggcactgcgc cggatcaccg tcgtgagccg tctcgggcgc
     2281 gtcgatgttc agcaggcgca gctttgtctc agcaccgtcc agccggacgt ccagggtgtc
     2341 gccgtcaacc acgcgcagca ccttcgcctt ggcaaacccg gaaacatcct tggtggcctc
     2401 ggcggcagcc gtggtcgaac tcgccgtggg agcgggcagc agccccggcc accaccgcgg
     2461 cagcgtgccg atgacccaga cgacgaggcc gatgacggcc agcgtcgcga gcatcgggaa
     2521 caccactcgc cgcaacacga tctgcacgct atcaagaccc actgacacgc tcagggtgag
     2581 cccgcacctc tcccccaaac cgcccccgcc aagttccgtt ccccggcctg gttggcctaa
     2641 cttcgaacca tggccaatcc cattgaaccc agtgacgtcg gtccggccgt cggtggccac
     2701 atcgccgtat cggacgccga ccgtcgccac gtgatcaccc tgctcaactc ggcccaggcc
     2761 gagggccggc tcaccccact cgaacgcgac gagcggatcg ccgccgcgga agcggccgag
     2821 atcttcgacg acctggtgcc gctcacccgc gatctggtca gcccgaccgg cccggcagcg
     2881 cgtccggtgg tgaactacga tgaagtacac gcatcgggag ccgccgacca gatcgtcgcg
     2941 atcttctccg gcgcaacccg caagaacgcg tggcgcgtcc gccgcaacac ctcgatcatg
     3001 gccgtcttcg gcggcgtgga gctcgacctc acccgcgcca cctttgaggc gcatgagctc
     3061 agcttcaacg tgttctgcct cttcggtggc gtcgaggtca ccgtcccgcc gggcaccgac
     3121 gtcgacagcc aggtcatcgc cgtcttcggc ggcaccgaag tcggccgcct gaccccgccg
     3181 gacccggcac tgccgaagat cgtggtcaag ggcttttgcg gcttcggtgg cgtccaggtg
     3241 cgcaacccga aggcccacaa ggaccgcaac tgacccggac tggtgttgac ttcaaccatg
     3301 accgatctgt tcaccccgac ggccacttgg cagcggctgc ccgaacgctg cgcgaccgcg
     3361 cggcgggtaa gcgccgctgt ctccaacctg atcgcaaccg cggtggtcgc tctcccgctg
     3421 ggcttcttct tcgactggcg ctgggccgcc ggcctggctg tcgccgggct tgcctggacg
     3481 ctgtggcgag tcatccgggc cggacgttgg gtgcgcaact tcgggtacgc cgaacgcgcc
     3541 gaggacctgc tgatcaccca gggcctgtgg ttcaagcggc tcaccgcgat cccgtacggc
     3601 cgaatgctct cggtgaacgt cgaatccggc ccgctcgatc gcgcctgggg attggcgacc
     3661 gtcgaactcg tcaccgctag cgtccaatcc ggcgcccaga ttcccggcct cgcccaagcg
     3721 gacgccgccg cgctgcgcga ccggctgatc gccgccggcg agagccaggc cctgccgcta
     3781 tgacgttgcc gccggagccg ggaccgctgc cgccggcgcc accgccaccg cttccacccc
     3841 agctgcaacc gccagaaccg ctgccgcagt acgcgccggc agtcggcccg gatcaaccca
     3901 aggcccagga cccgccggca tcgacgaagg tgtccgagcg accgcacccg ctgacgccct
     3961 tcgtccgcgg ctgggtgctg atcctggcga tcatcttggg cgtcggcagg gacctgctgc
     4021 agcaccagcc ggacgaaggt ccgctaccac cgcttgaatg gatcgcgtcc ggcatcggcg
     4081 cggccgtcct gatcgcggtg ggcatcggct tcgtctcgtg gcggttcacc cgcttcgtga
     4141 tcgacgagga cgaactgcgt gtagacaccg gtgcggtgtt caagcgctcg gtccgggtcg
     4201 cattcgacaa ggtgcagtcc atcgacgtcg tgcagccgtt cgccgccagg ctggtcggtc
     4261 tggccgaact gcagatcgat gtcggtagtc aggagcgcgt gcggctgcgt tacctgaagc
     4321 tgaccagggc ctatgcgctg cgcgactatc tgctggccag ggcccgcggc aaccgggtgg
     4381 ccgcaaccga acacgtccag gcgagctcag tgctcgcaga cctgaccgag catgacgagg
     4441 tgctggtgca ggtaccgccg cagacgctgc tgctggccgc ggtcacctcc cacgagttct
     4501 tcagcatcat cggcggcggc ctggtgagcg cggccgtggt gacctacctt ggctggggct
     4561 ggggcgcgct ggccattctg attcccgtag ccagcggcct gttcagcttc gtatcccggc
     4621 ggatcatcgg gcagttcaac tacaccctct cccgccgcga gcacgggctg cgggtgtctc
     4681 gcggcctcac cagcctgacc agccaatcgc tgccgccgcg gcgcgtccag gccgcccaga
     4741 tcacccaatc accgatctgg cgcaagctcg ggctctaccg gttggacatc gcggtgatcg
     4801 gctggggcgg catcagccgc gacgagaacg acaccggggt gtccacgatc ctgctgcccg
     4861 cgggcagcat cgaccaggtt cgggcggccc tgagtgcgat ctggccgcgg acgaacttcg
     4921 agaccatcgc gttgcagcag ccgcccaagc gggcacgctg gctgcatccg ttcgccgtgc
     4981 cgttcatgcg ctggggccac gacgaccaac tcacggtcac ccggcacggc tggctagtgc
     5041 gcacctggca tctcgtgccg cacgcgcgga cgcagtccgt ccggatcacc caaggtccgc
     5101 tgagccgccg cctgcgcctg gccgaactcg agttccacac cgccggtggc acggtgcagg
     5161 cgcgggcgcg cggactggac gccgattcgg tgagagactt gcaggccgag ttgctggctc
     5221 gcgtccacca cctgccggag ttggacctaa gcgaaccgac cccgcagcct gagccgtcgg
     5281 agtcgctagg agatnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntggcgt
     5401 cgtagacctc gtccgtggcc gagccgacca cgttgaagta gcgcaggctc accccgcgga
     5461 agccatcgtc ggcgcgggcc acatcgttga tcagccactc cccgatcagc ttggattccc
     5521 cgtagggcga ttcgggatgg ttcggcgaat cctcggtgac cagctcggtg gtcggcgtgc
     5581 cgtacaccgc agcggacgag gagaacacca ggttgcgcac cccgactgcg gccatcgctt
     5641 cgaggacgct tgccgtgccg gtcacgttct gcgcgtaggt gtgcagcgga cgctggaccg
     5701 acactccggc gtacttgtag ccggccacat gaatgacacc agtgcagccg tggtcgcgca
     5761 gcgccgcggt aaccgtggcc gtatcgagga tgtccgcttc gacgaacgga accccgtccg
     5821 gcacgaacgc cgtgaggccc gaggagaggt tgtccagcac gaccgggccg agtccgcgct
     5881 gctgcagcgc cctggtcacg tgtgctccga tatagccggc gccgccggtc actagccaac
     5941 tggtcacgat tgctgagtct agggcgtcag tgtggaggat cttgcaccgt gttcggtgca
     6001 agatcctcca cactgacgcc ctaatctgtt ccccatgcag aacagagctg tcctgggaat
     6061 cgatatcggc ggatcgggca tcaagggcgc cctcgtcgac ctggccgccg gcgatttcgc
     6121 cagcgaacgc gtccgcattg gcacaccggc ggagtcgacg ccggcgaatg tggccgatgt
     6181 ggtcgccgag atcgtcgagc agttcgccga ccagatgggc gacggaccga tcggcatcac
     6241 cattccggcg gtggtcaccc acggcgtgac ccgatcggcg gcgaacatcg acaagtcctg
     6301 gatcgacgcg cccgcggagc agatcttcga ggagcggctc ggacgcgacg tcgtgctcgt
     6361 caacgatgcg gacgccgccg gcgtggccga ggtggtctgg ggtgccgcga agggcaacga
     6421 cggcctggtc atcatgacca cgctgggcac cggtatcggc accgcgatca tccaccgtgg
     6481 cgtgctgctg ccgaacaccg agctcgggca tctggagatc gatggccatg acgccgagtc
     6541 gcaggcggcc gcgtccgtgc gggagaacct gaagctcagc ttcgacgagt acgtgccgcg
     6601 actgcagcgc tactacgagc ggctggaggc attgttctgg cccgacctga tcgtggtcgg
     6661 cggcggcatc tcgaagaacg cggacaagtt cctgcccaag ctgaagctga agaccccgat
     6721 cgtgcccgcg gtgctacgca acaaggcggg catcgtcggc gccgcctggc tggccgagca
     6781 gaaggccgag tcccactgaa ctaaaagggg acggttcctt ttccgttcac cttgtccaca
     6841 agagcgcgaa gatctggcgc gaagctgtgg ataacttgaa cgaaaaaaga accgtcccct
     6901 ttacgttcac atgcgctcgg gcgcctgaat gccgagcagg tccagaccgg acgcgaggac
     6961 tttcttggtc gcggcgcaga gcgccaagcg ggacgcgcgg acgtcaccct cactcttcag
     7021 caccgggcac tgttcgtaga acgagctgag cgcgccggcc agttcgtaca ggtagccgca
     7081 cagccggtgc ggctgcaggg tctccccgac cgcggccacg acctcgccga atcggctcag
     7141 tagcagcgca acctggtgct cggccggctc agtcagcacc gtgacacccg cataggtgag
     7201 cccctcggcc tcggccttgc gcaggatctg gttcacccgt gcgtgcgcgt actgcaggta
     7261 cgggccggta tcgccggtgg tggccaccat ccgctcggcg tcaaaggtgt agtccttctg
     7321 caggccgctg gacaggtccg cgtacttgat cgcggccagc gcgatcggcg gtgcggcttc
     7381 cttctcggcc gcgtccagca gcgagttgag cgtcaccgcc gtcccctccc gcgtggagaa
     7441 cttcttcccg tccgcgccca gtacctggcc gaagccgacg aactcggcgg acgtggtctc
     7501 gggcaggtag ccggcgatcc gcgcgaccgc gaagacctgc tcgaagtgga aggcctgcgg
     7561 cgcccccacc acgtagatca tccggtcggc gcccaggtca cccacccgat ggcggatcgc
     7621 ggcgaggtcg gtgcagctgt agccgaagcc tccctgggcg ttgcggatga tcgccggggc
     7681 cgggaacccg tccacgaaga cgaccagcgc gccgtcgtcc accaccgcga tgccgcgtga
     7741 ctccaggtcg gccgcgacga ccggcaggtc ggcgttgtag atcgactcgc cggccagatc
     7801 cgcgttggtg agcaggacgt tcatccgctg ataggtggcg ttgaagccgg ccagcgacac
     7861 gtcgatcagc tgctgccaga tccggcgggt ctccacgtct cccgactgca gcagcaccac
     7921 ccgagcccgc gcgcgatcgg cgaattcctc ggattccttc agatgcttgt tcgcccgctg
     7981 gtagagctcc tcggcgccga cgaggtcaag cgtggacacg tccaggccct cgtccaggat
     8041 ctcctctacc agcatcccga actggcgtcc ccagtcgccg atgtggttct gcggaatgac
     8101 cgtgtggccc tgcgcccgca acacccgggt gaagcagtcg ccgatgatgg tcgagcgcag
     8161 atgccccacg tgcatctgct tggcgacatt cggcgacgag tagtcgatca ccacggtctt
     8221 cggctgtaca gccggggtga ttccggctgc cggctcggtg agcacctggg acgcaaccgc
     8281 tgcgagcacg tccgcacgga tgcgcagatt gatgaatcct ggcccagcca cttccagcgg
     8341 ctcgcaaagg tcgtcgagct gaatcttctc gaccagggac gccgccactt cgcgcggcgg
     8401 tcggccgtcg gcgttcgcca gccggagcgc cacgttggtc tggtagtggc cgaactgcgg
     8461 acgcgtcgcg ggtcgcagct ccggatcgat tccggcgacg gcggcaacac gttcactgag
     8521 cagggtcggc aaggacagca tgtgggacat ttttgcacag cggtgcgtag cctccgcgac
     8581 gctcccgctc cccctgttgc ggtccatgag cggtacccaa ccagccgcta cgacgcggac
     8641 ggtccgcccc ggtgtcgcga tctcgcggcg atcgcatcgg ctacgccggg ttccgccctc
     8701 ggctagcaca cgagcttcga gatcgcgaca ctaaccgctg cccgaattcc tggtgagcag
     8761 atcccagacc agcttcaggt tggcggccag ttcgcggcgg acgccgtacg cccaagtgcg
     8821 ggaactcgtg cgggcggtct catcgccgac gccccaggcg tccacaccga tggctcggca
     8881 gatcgccaac gctcgcggca ggtggtagcg ctggctcacc acgatcatcc ggcgcacgcc
     8941 atacacatcc cgggcgcgcc ggcaggaggc gtaggtgtcc agcccggccg ggtcgaccag
     9001 cagcgcgtcc ggttcgatgc ccaagccctc aaggtagtgc cgcatgccgc cggtctcgtc
     9061 gtagaagcgc gaccgcccgt cgccggagag cagcagccgc tcaaccagtc cccgccggta
     9121 caacgcagcg gccaggtcga ggcgcgcgcg cagaaaggcg gacggggttc cgtccgcgaa
     9181 gacctcggcg cccagcacca gcgcaaccgg ggtgtgcggc gcgcgctcca ggctgacgat
     9241 ccggcttcgg gtgatcaggt ggacggttgc gccgagccca agaacggcgg cgagcgggac
     9301 ggctgcgaca ccgagcccga acgccaattg gcagcgagta cccccaccgc tgcgcaacca
     9361 gaccatccag ccagcctaga cgcgcgcttc cagatcggcc gagaaagcct aagaccccaa
     9421 gtcagcgagc ctatgtccgg aagcggagaa ggtgcagccc gacccggcca atccgcggac
     9481 ggacatggta aattcggtgt ctaattccag gtccttgggc aggtcatgct cgtccatcat
     9541 gtttgggggt gcggtgcgca agcgagcggg tcggctggga cgcgtccttg ctggattcgc
     9601 cgtgctttcg ctggtcgcga ccctgctcac cctgctgcag tcgccgcgcg cgaccgccga
     9661 gccggtgctg ccgtccgccc ccgacacggt gacggtcagc cagcgatcca cgatggtgat
     9721 cgcgtcgtgg cccgtggtgg caggcgcgga cgggtacacg gtcgaactcg acaccatgcc
     9781 gactttctcc gcaccccgac gcgtcgtgac cgccgacacc atgtcggtgg tcaccgggct
     9841 cacccccgac accggctact acctgcgcat cgccagctgg gcggaagatc ccagcacgtt
     9901 cggtagttgg agcagcctct acccgttcag caccggcccg caggagttcg ccaaactacc
     9961 gcccgtggtg aagaccgaca gcctcacgtc cacgtccatc accgccgagt ggacggacgt
    10021 cgataaagac ctgagctacg aagtggccct tggcaaacag ccggacgacc tgaccgcgac
    10081 gaagcaggtc aaggaccaga cggcgacgtt cgacgagttg gagaaggtca ccaagtacta
    10141 cgtcagcgtc cgcgcggtgg acgaggccgg cgcgccggtg accgcctggt ccgatccggt
    10201 ggaccagaag accgcgaagg aactaccgct gcgggtgggc agctacaaca tcctttgcga
    10261 gaactgttcc ggcggtaagg cgacctgggc cagtcggcgc ggaccgctgg tggacgcgat
    10321 ccaggagcag gacctggatg tgctgggcgt acaggaggcg tccatcggca gcattcccgg
    10381 cggcggcgcg caatacatgg acctgatcaa ccgcctcggc aagccgtaca agctcaccga
    10441 gtacgggcgc aagatttctc ccgacgtccg gattgtctac aacagcgagc gcgtcgaact
    10501 ggtcaagcag ggcactatcg cgctcccacg cggcgactcg gcacggttcc tcaactgggc
    10561 cgtcttcgag cagaagtcga gcggcaagcg gttcttcttc acgaacacgc accttgagcc
    10621 gaaggacggc aagaagtact ggaacacccg taatcggcaa gccaaagcag tcgtggccgc
    10681 gacgaaaaag ctctccggcg acctgccggt ggtgtccgtc ggcgactatg cctccaccaa
    10741 gtgggacgac cccagcaacg gcccgtacga catcatgcag tccgccggct acaaggaccc
    10801 gctcggcaat gccgcccgct cccgcaacag cgcgcccggt gagttcgtgg agaagcggat
    10861 caacacgtcc tacgcgagcc tgaacatgta caagcgccag gcccgcaact tcgccggtga
    10921 cgtgaacggt tcgaacaccg actacatctt cgtctccacc atgcgggttc ctgagtacga
    10981 agtcgttgtg aaggtgaatt cggccggcca gttcgtcggc gtgatcccgt ccgatcacaa
    11041 cctgatccgc gccaccgtgc tgctgcccta ggtccggtca ggttggttca gccaagcttc
    11101 ccgtgaacgg gtggccaagc ttgtcgatac ccctcgcccg ccagctgcgg tgggcttaag
    11161 caccgctcaa gggttccgcc gggaaggttt cggttcagcg cgctggccgg gttatggtcg
    11221 agatccagca ggccactttc aggagtttca gctgatgctg cccaaatcgt ccgtcaccgt
    11281 ccgtttgcgt gctgccctgg taggcctggc gctggtactc ggtctcgtcg tggccaatcc
    11341 cgcgccggcg gaagccgcac cgaaagcacc gtccggactg acccgagcaa gctactcgaa
    11401 caacgccgtc cggctgacct ggaaggcggt caagggggcg gccgcgtacc aggtgaagta
    11461 cgcgagcaac tccaagctca agaaggcgtc gtacgtcacc gtggccgcgc cggtggcgga
    11521 gatcggcggg ctgaaggcca gcaagaacta ttacttcaag gtgaaggcgc tgaaggctga
    11581 cgggaaggcg ctcaccaagt actccaagac caagaagatc aagaccagct cgacgaaggc
    11641 atacacggtg ctgagcccat cggggctggc actcaaggcc aactacggcg atcaggtgaa
    11701 cctcgcatgg acggcacaag gatccacgaa ccggtaccgc gtcaactggt caacgagcaa
    11761 gtcgatgaag aaggccaagg ccgtctacgt gaccggcccg aacgcggcca tcggcggcct
    11821 cagccggtcc aagaactact acttcacagt tcaagtgagc agcgccggca ccaaggcgat
    11881 cagcccagcg accacggtgc tgaaggtgaa aaccaggaag accgtaaccg cggcgtcgcc
    11941 catggccacc gtctcccgag tcgagccaac agccttcacg gtctcatgga agcccgcccg
    12001 caccgcccct gcctacgaag tcgtatacag cgcgaccaac tgggcggaca gttcaagggt
    12061 cgtcgcggcc ggcacctcgg tgaaggtcca aggcgtattc gccaaaagca cctatcaggt
    12121 gaaggtccga gcgctcgact ccgccaacaa gcccgtcggc gggttctcca cggtggcctc
    12181 tgtcaccaca ccatccggcg aggaaagcct tcgcctggcc agctacaacg tgcgctgttt
    12241 tgactgcaaa ccaaagtcgc aaagcggcac cccgattgtc gacctgccct ggacgcagcg
    12301 ccggagtctg gtggcccaaa acatcggtga agccaacccc gacgtgatcg ctcttcaaga
    12361 ggcgcagcag gcggaactgg gcgatggcag caagctgaat cagtaccagg acctcctgct
    12421 tgccatgaac gaacagctgg gcggggtctg gaaactgact aatgaccgcg aatacaactg
    12481 cgtcaagagc accactccga ccgcctgcga acccaaggat cagggcgcgt ccaagggggt
    12541 gcggatcgcc tatcgaatcg atcggctggc aatcctgcgg gaaaacggtg tggaccgcac
    12601 cggctcgatg ctcctgccga cagcgcccgc ggacggcagc ggggcgagct tgaaggccag
    12661 cgagcggtac atggtgtggg cattcatgca acaactcacc acgaagaaga cgttcctgat
    12721 ggtggacgtc cacctcgagt cacgtgacga cacaaccagt tccagcgcgg gcgatgccgg
    12781 ttgcggtgac acggcgaatc cgatctgcgg gaaacttcgc gttgagcagg cacaggcaat
    12841 cgtcgcgaag atcgcccagc tgaacactga tggcgtcccg gtagtcgtcg ctggtgactt
    12901 ctcgtcaagc cgctcggagg cgagcaagct gttcccttcc gatgtgttca cggccagcgg
    12961 ccttgtgaac ccgctcggcg gcattgagaa gtactacgac ccggacgcgg tcccggccaa
    13021 cacgcgaatc aacatcgttt acaacagcgt caatcagctc gagcggacgc ctcgccgcta
    13081 cggcagtgac ccggcgaaga acaacactta caacggggcc tacaacgact acatcctcac
    13141 cagtccgatg gccgtttacg acttcgagca actggttcac ctgaatggcg acggcaccta
    13201 cgccgaccag cagttcgcct cggaccacaa cctgatccga atgacagtcc gtctccccta
    13261 gccgttctcg cgagcccaaa gtcggggacg gttccttttt gcaccacaag tggcccctga
    13321 ctagcgcttg gtgcgttatg gaaccgtccc ccgactgcac aaggcctagt cagaggtaag
    13381 ttgcgatgca gaaaggaacc gtccccggcg aggggaagcg ctacttcttg gggagggcga
    13441 acaagcgctc gatcacccgc tcggcggagt ggccgtcgtc gtgggcgttg aacttctcgc
    13501 gccaggcgac gtacttctcg gcgtagccgg gggcgtccga ttcagcgttg cggatcgccg
    13561 cgacgaccgc gtcctgggtg aacagcaccg ggccgggcgc ggtctcctcc aagtcgaaat
    13621 acaccccgcg ggtctggtcg cggtactgct ccaggtcggg cacgaagaag atcatcggcc
    13681 gcccggtcac cgagtagtcg aacatcaccg aggagtagtc ggtgatcatc atgtccgccg
    13741 ccaggaacag gtcagtgacg ttggggtagc tggtcacgtc aatcacgccg ggcacccgga
    13801 cgttgccgtc ggtgcgtagc gtccgggaat gcccacgcag gagaatgacg tagtcatcgc
    13861 ccagtttgcg ggccatgtca ggcaggtcga ggaagtccac cagggtctcc tcggcatcat
    13921 cccgccaggt cggcgcataa agcaccgcct tctggtccgg tcggattccc agcagctccc
    13981 gaatcggctc acccgacttc gtcgacatgt cgtcgttgcg agggtagccc tcctcccaga
    14041 tcggcccgtc ccaggcgtac gccgagcgga agatcgccgt cgagtgcgcg ttctgcgaca
    14101 acaggacgtc ccacttcacc cgttcgtagg ccaggaacgt ctgctcgtcc ttgctgaagt
    14161 tcggccgatc cagcccgatc cgcttgaaca tcgacccgtg ccaggtctgc agcaccacct
    14221 ggaacggctg gtgcttgaac ttctgccgga tccactcgtt ggtgatgatg tagcggcacg
    14281 tcgaccgcac gtcccaccac tgcagggtgc ccttgagtac gggaatcgcg ccctcgggca
    14341 cggccactga atagtccagc acgctccagt agcgcttcag ctcggggaac cgccgggcca
    14401 cttcgcggtc gagtgcgtag gtgttgcacg tcgccgcccg gccgttgaag ctctcgaagt
    14461 acaccgcttc ctgcggctcg aacgtgcgcg cgtggtactc ggccttcaac cgggcctgct
    14521 ggtagtcgcc gaccagctgg tcctgcaacg gcgcgctgaa cagacaggtc acccggcgtc
    14581 cggacgccac ccccacccgc agcttgatca actcacccat ggtgcgcagc ggcgtccgct
    14641 ggatcagcgc cgacgtggcc ccgacccgca gccaggcccc cgcagcggtg cgtccgcgca
    14701 gcacgtacgc gccggtctgc ggcggcagct tcggcccgcc ccaggcgctg atcagcagtg
    14761 gcgcgaccgc ggtgaaccgg tcaccgtcga tggtcagctc cacttcgagg tcctgccgcg
    14821 gaccggtcag caccagcttc gcgccctcca gccgaccgag ggcatgcccg gtgtagcgaa
    14881 ggctaagcgg ctcgtccacc agttcgacgt cgtccaccag cagcatggcc gcggtgtcgc
    14941 ggatcaacag cgcgctgccc ggcccggtgt aggcgtagcg cgcctggccg ggcgtccact
    15001 cggcgtcccg ctcgtccaag ccgctggcca cggttgcctt attgccagcc gagtcccaca
    15061 cctgcaccag ccagtcgtgc accgccagcg aagcacccag atcctccggg ttgttcttct
    15121 ccagatcggg ctcggcaacg ttggcgactg cggtcggcgt ggccagggtc gcgctcagcc
    15181 ggtagcgacc agcacccagc gactcgacgg ccagcggcgc cttgtctgct tcgaccagcg
    15241 cagcccgcgc cacctcgaac tcgggcgcca gcaccaccaa ttccacccgc tcgccgtcga
    15301 aacttgccgt ctcggcgacc accggcggct gacgcagcag gaacttcagc cccgtctcct
    15361 tgcctgtcca caccggctcc agctgccgat cacccgctgt gcgtgccacc aggaacgcac
    15421 cggctgcggt cttggcccgg ttcttcaggg tgccggtgag cttgcgctcg gtgtcgtcaa
    15481 cggtcacccg gacggtccag ctgtcgccga tctcaccggc caacgcggac gcgaggtcga
    15541 gcgtagccac gaacgcgtag ttgccgtagt cgaccggcat cagccgggcc cggatgttcg
    15601 ccgccgcgtc gtaacgctgc gtggccttgg cccggatctt ctgcttggtg cggtcattgg
    15661 tgcaggtcac cgtgaccgtc ggcaccacgt cgttgctgcc cgtcccacgc tcgtaggccc
    15721 aaccgaccag ctcgaaggag gtggtgccga cccagcgtcc agactgcatc cacgccaccc
    15781 agccgacctg cttgccggtg aagcggttga cgaccgcgcg atacgccttc cgcccggaaa
    15841 gatagccggt gtacaggaac gcgaaccgcg acctccgggt gcgccggatc agggtttcca
    15901 gtcggttgta cttgggggcc acagttcgcc taactacttg cctgagtcca gtttgcgggg
    15961 caaacggacg ctcactcgaa ggtctcggcc tggctgcgcg cctgctcaat gatgtccgcg
    16021 atcgcgatcg actcggccgc gcggagtttg aacgactccg gcgtgccgtt gcggatcatg
    16081 aacgcgaact cggccagttc gtagcgaagg ccgtcgccgt cgaacttgta gtagtacttc
    16141 ttgttcctcg actggtcctc gaagcgggtc tcgaagtact ccgtcagcca ccacggcgcc
    16201 ggcacataca cgtagccgtt cgaccccgcg acgatcagct cgccctcggc cttcaccccg
    16261 atcccggtac gagcggacgc gatcccgccc gggaagtcca cgtcaatccg gctgaacacc
    16321 tcgaccggcg agccggccgg cttcagcgaa tgcgtggtta ccttggtgta gtcggtgccc
    16381 agcagcttga ccacggcgag cagcggatag gtggccagct cggagatgct gccaccgtcc
    16441 tcgccctcaa gctcgcgacc ggacttgacc agcttggtga acgtcgcgtc caccgatcgc
    16501 acctggccga tggaaccgct gcgggccacc gccaccatgc gctggaagcc gggcgcgaac
    16561 gcggtcttca ccgcctcgag cagcaccacc tcgtgctggg cggcaagctc aaacagctcc
    16621 tcggtcgctg ccttggtcag cgtcatcggc ttctcgcaga gcacgtggac gccggcctcg
    16681 atggcctgcc gggcgtagtc gtagtgggtg gcgtgcgggg tggcgacgta gaccgcgtcc
    16741 acatgcttga gcaggtcggc gtacgtctcg ttggcccact ccagctcgag cttctcggcg
    16801 aacgcctcgg ccgattcgaa gcgccgcgag tagaccgcct ccaccgagac accggagacg
    16861 tagcgggctt cggcgacgaa gcgtgcggcg atccgtccgg aaccgaccac gccgaggcgg
    16921 atcacaccgg cgctctcgtt gcgcagctgg gtgctggaga cgcccttcgt ccggtcgagg
    16981 tagaccactt tgcagtagtc gccgaggtag tcgaacttac ccagccagtc ggagccgatc
    17041 gcgaagatgt cgacgccgtg cttctggatg tcctggacct tctggccctc gtactcctcg
    17101 atgatgatct cgtcggcgag gccgctgtcc tgcacgtttc ggatgcggtc catcagtgac
    17161 tggcggacgt tcagcttgcc gcgggagtcg tcgtagttct ccgtggtcac cccgacgatc
    17221 aagtagtcgc cgagagcctt cgcacgctcc agcagccgcc ggtgcccttc gtggaacaga
    17281 tcgaaggtcc cgtaggtgat gacccgcatt cgcgtccttc cagtcctggc cgcaccccgg
    17341 ccggcgccgg ttggccacgt gcttagattg tggcagatag ccgaaggagt caccctgtga
    17401 agctgcccgg accgctcaat cgaatcactg ggaggggcgc ggacgcagcc cccgccaccg
    17461 gccgaaccga ccgccgtccg gacgcccgtc cgctcgaccc ggcaccctcg ctgcagccct
    17521 tcccagcggc gcgctacctg tacgcggtca acgcgatccc ccgcaactac gccggacgca
    17581 gttcgtcgat catcaccaaa gcgcgggtgt tcgccgagca ggccggcgtc cgctcgaccc
    17641 tggtgacctt cctgcactcg gccgaactcg ccgacatcga gcacgacctg cgtgagcgcg
    17701 gggtgcttgg cgatcatctc gacttcgtct gcctgcacga ctactacccc gacgagtcca
    17761 gctacaccgg cgaaccggtc gagcacccga tcgacgaccc ggagctgaac tggctgaagg
    17821 agccgaccca gccggtctac cgctacttcg acagcaccgg cacctaccgc cgctatcgcc
    17881 gctacgacca cgccggacgc ctgatcgtgc aggaccattt caacccaaac cgtggccgga
    17941 cgctgcgcga ggagttccgt cccaatggga cgctgcgccg ccgggtctac atggacctgg
    18001 cccacaacct gccccggcag gagatccact accgtgccga ccagaccccg gcgttcaccg
    18061 tctggtgggt ggtggacgcg gacgcacagg agtcggtggt cgagaaggtg atcacctttg
    18121 acgaggccgg acggccgacc ggcatggccg acagcatgga cgcgatcaac cacctctgcc
    18181 tcgaccggct gatcggggat gagcaggcct tcgttatggt cgaggaccgg catgtcgacc
    18241 gctacctgct gagctaccag cgtccccacg tacggatgct gtacgtgctg cacaacgcgc
    18301 acatcaagga gccgtacgac gacccgaacg cgatccggcc gagcttccgt ccgatcctga
    18361 ctgcacgtca ccgggtggac gcgatcgttt tcctcaccgc aacccagcgg gccgaggccg
    18421 aggccaagta cggacgcgcc gactcgttcc gcgtcctgcc ccattcggtg gccgcgcccg
    18481 agttgacgcc gggggtcgcg cgcgaaccga acctggtggt gatgatggcc aggctggaga
    18541 gcaagcagaa gcaggtgaac cacgcgatcg aagcgttcgc gcaggtggtg gcgcagttgc
    18601 cgcaggcccg gctggagatt tacggccgcg ggccggagca ggccgcgctg cagaccctga
    18661 tcaacgacct gggcgtggcc aagcaggtca agctgatggg cttcaccaag gaaccgcacc
    18721 tggtctacca gcgcggggcg ctgtgcatcc tgaccagcag gttcgagggc gccccgctga
    18781 ccgtccagga ggcgatgagc tacggctgcc cggtggtcag ctacgacctg cgctacggtc
    18841 ccgccgacgt gatcaccgac catgtggacg ggctgctggt gccttacggc gaccgcaagg
    18901 cgatggcggc cacggtcgtg gccgcgctgc gcgacccgga actactggcc aggatgtccg
    18961 ccgcagcccc caagcgcgca gccgagttcg gcgaggcacc cttcgccgcc cgctggagcg
    19021 cactgttcaa cgagttcgcc cccaagggct gaacgatcag ccctgctcgc gctggcgtcg
    19081 ccgccaggcc aggaggccgg cgccgccggc cagggctagc gcgaccgccc cgccacccag
    19141 gatccacgcg gtggagtcac cgaggtcgac cggtcccacc ggtgcggggc tggccgacac
    19201 agtcggcgtg gccgccggtg acggcgtggc gatcggagtc ggggtgggcg tctcggacgg
    19261 ggttggcgtc ggtgccggtg tagccgaggg gtcctccacg tcgtcgagga tcgtgggctg
    19321 ctggtcctcg ccctgccaga cctcagactc cagcggcgtc caactgccct tgccgtaagc
    19381 gaattggaag gagccgccac gcttgtcgcc gaactcgtcc accgtccgat agatcaccgt
    19441 gtaggtgccc ttcgggagct tgctgcgcaa ctgggtggtg acgttggtgc cttcgacgat
    19501 cagcgggccg gtggtcacgt tcgcgccgct ctcgtcaagc accacgatcg tagccagcga
    19561 ggggtccgag tcggccttga aaaccagggt cacccagccc ggaacccgct tcaattcctg
    19621 ctgcggagcg gggtcgctgg agtccaagaa gtcgtccgct gcccacgccg aaccgtgccc
    19681 gatgaatccc accatgagta gggcggacac catgacggct agccagcgtg cactcttcac
    19741 agcaactcca tagtctgcta ggcagggtcg aacctgttta cagggtagcg cgcagcgtga
    19801 gttatgctgt cttgaaagca ccctaagaag aattctcagc caccgggccg ccggctcgat
    19861 gatggcagcc acgacgaggg ggacggatgc cttcagccac gccacggaaa cgatggaccc
    19921 atcgacttct ggcggtgttc ggcgctgtgt ccctggtcgc agcactggcg accacaccgg
    19981 ccgccgcgat cgactggacg atcaaccccg acacccagga ggccgcagcg gcgacgaacg
    20041 ccagcgccga cgcttccagc tgtcccacgc tcaccacggt gccgggcggc gtcgaggcct
    20101 ggttcaacac cgacgacatg gagtaccgcg gcctgggtga caccaacaac gccccgtccg
    20161 ccacgtggag cttcgcccac aaggtcgcgc agatcatctg cggcgccaag cagggcgcca
    20221 acatctacgt gggcatgtac ttcatccggg cgatcggcac ggccgagcgt cccgagaccg
    20281 acaccgacgt catctggaac gcgatgaagt acgtgatcgc caaccgcggc gtgaagatcg
    20341 gcttcgtgat ggacggcggc ggcatcacgt cctccagctc gaaggcacag atcaaaaaga
    20401 agctggccga gatcggcgcc ggcatctact ggtgctacaa cggctgtctc aacaccaata
    20461 cggcgaagaa attcccgtac gcgatcaacc acgagaagtt cctcgcgatc agcgacacga
    20521 tctgggccgg cgacagcgcc cagcaccccg cggtctactc ctcgtccggc aacttcgccc
    20581 gcagccaggt gcgcaactac tggcaggaag ccacgctggt ctacgacgac gtgcgccttt
    20641 acaacttcac cgtcgaccgc tacaacgcca tgaaggtttg caccggcagc agcaccggcg
    20701 gcagcaactg caagtcgggc aagttccccg gcgcgaagca gtccggcatc gtcaccgcgg
    20761 ccggcagcct gaagaagctc cgcggcatct ggaccgattc gtacttccgc cgctacaccg
    20821 acgccggccg cggcaccaca atctccttca gcccgcagcc tgagaccgtg ctggactact
    20881 acgtcgagca gttcaacgac gtcgactgca ccgtggacaa caagatccgg gtggcgatgt
    20941 atcggctcac cgacacccgc gccgtccgct tcatcgacac ggtgaagacc ctgaagaggc
    21001 aaggctgcga cgtgcgcatc ctgctctctc agaccggcgg cgcgcagacc atctccaagc
    21061 aggtctccaa gcagatcaag gccgccggac tgaccgagca gatcaactgc acgcggatcc
    21121 cgatccacac caagatgatt ctgatcgggc cggccaccaa caacaacgga cgcatcctgt
    21181 tcggcacggc caacatgagc acctcggcca tccggtacag cgaggagcac gtgatcacga
    21241 tggactcccg ccgcgccgac gatgccatgg ccgccgacat ccgccaggtc tacggggtct
    21301 accaggccgg ctggaacgag ctcaaccagg gaagcaggac gtgcaagtga acaccaatcg
    21361 cacccaccgg cacagcccct ggttcgcgct gctcgccgcg ttcgtggtcg tcctcggcct
    21421 gctcgtgccc gccgccaacg cggacgcagc caagtacccg tacagcgctc cgccggagct
    21481 ctccgcagtg aactccgcca agacctcggt agagctcttc tggcgcacgg tcaccggcgc
    21541 acccggctac cgcgtccgcg ctgtcggcgg tggccagacg ctgtaccaga acaccggctc
    21601 ggacggcacg acggtcttca agggcctggc cccgagcacg tcctacacct tcaacgtggc
    21661 cgtcctgcag gtcaccgacg gcaagcagct aagcgcctgg tcgaaggcga aggcgacccg
    21721 cgccaccacc gcggcgacca tgctcgagcc gcccaccgac ctgaagatga ccaagcagac
    21781 cccgaacacc ctgaccttgt cgtggacgcc gccggccggc ttcgacccgg ccgcccacgc
    21841 catccgggtc gactacgccg aggaccaggc gatgaccacc ggcaacggat cgcagttctt
    21901 cagcggtaac tccggtacgc tcaacgggct gagcaccaac accaacttct tcgtccgcgc
    21961 caccgtggtc ggcatcaccg accacgccca ggtcggtgac cgcagtactg ccgtgctcgc
    22021 caagacgctc tccccgattg gctggatcca cgggactgtg accggagcgc ccgacgccgc
    22081 gttcgcgaac tactcagtag ctgcgtactc ggtggccagc ggcgacgtga accagcaggt
    22141 gaacctgacc aagaagggca gcagctggga atacacgctg caggtgcgtc cgggcggcta
    22201 ctacgtgcag gtggtcaacg tcggaccagc caactacacc tccctgtggg ccaagtcggg
    22261 tgccgacggc gctctaatcc agagccaggg cacccaggtg agtgtggcca tgggtggcac
    22321 taccgaggcg cccgcggtaa ccgtgggcaa gggcaccgtc ctcaagggtc agatcgactg
    22381 ccccgggcag ggcacgaagg acagctgctc gatcgacgtg agcgcgctgc tcgacggtca
    22441 ggtcgtggcc gccaaccgca gcgattcgtc cggcaagtat gagatcgcgg gcctgcctgc
    22501 ggcgacgtac accgtccgca ccagtcacgc cgaggaccgg ttcgtggccg caaaggaaac
    22561 cgttgcggtc gctggtgaca gcgtcaccta caaccacacc atggctaagc gcgacttcct
    22621 caccacctac aaggtgaagt tcgtcaagaa gggcaaggtg ctgaagtggt cgagtcgcgc
    22681 ctacctggct gcggtgctgc ccaccgagcg cgccgacaac aacgacttcc agtggtaccg
    22741 caacggttcc ccgatctcgg gtgccaccgg cagcagctgg cggatctccg ggggcggcaa
    22801 gatcaagctc tgcgtgcgct acggaagatt cggcttccac accgcttcct gggcgtgctc
    22861 ttcggccaag aaggtctagt cacataaggt tgagtccatg ctgaagcacc cccggcgctg
    22921 ggttcgccga gtgctcggcc gttcgaagac cgatgcgcaa cccgccgtcc tgcggctgga
    22981 gttcagcaca cccgagcagt tcgccgcgtt cgtctcggat ccacgggcca ggcgcaagta
    23041 ccgcgccctc gaactgcggg tctcgccctg gtacgcgatc cgtccggatt ggacgggacg
    23101 gctggggccc ctgaagggcg tccgttcact gcggacggac ttcgaccagg ccggtcgggt
    23161 ggcgagcgcc gggctcgtcc tggctgaacc gatcgccggc tacgagctgg tgcgtgcggc
    23221 cttcgggctg ttctacccgg tgcggcccac gaccgggtgg ggcggccggc gggtggccgt
    23281 cgatgccggt gctccggtgg cggactgctg gcagccgggt ggcggcctgc gtccgcttaa
    23341 tccaaggaag cagaaaaagc agttcaccga cttcgaggtg ttggtggacg gcgagggcga
    23401 gttctggctg ggggagcatc cggcgccacc ggtgctgatc gagcggacac ccgggtcgca
    23461 aatcctgatc gatccgaagg tgcaccgccc gctggaacgc cggcagccgt cacccagcac
    23521 cgtttccgcg acagcgcggg tgcgggatgg ccgcctgctg atcgactcga acggctcgct
    23581 cctggtcgat gcggacgcgt ccgcgggcct caccgccacc aacctgcgcc agttggggct
    23641 ggtcaccgac atcgacgcct ccggcgtcgg tggcgggctc gtcgcgggcc gcctggtggc
    23701 ccaactggcg gcctatgggg ccgtcgtgca tgacgctgcc cccggtctgg aactgtcgcc
    23761 cgagctcgcg cggctggtgg aatcgccgtt cgcgcggatg ggactgctcg accacatgaa
    23821 ccggtcgctg gcccaggtgc gcgcggtgat gaccgggcat acccgtgcct tcaccgaggg
    23881 cgagctgccc tctgtctcgg tgatcctgtc gtgccagcgg ccgaacctgc tggtgcgcat
    23941 cctcgagcag ttggcggcgc aggaccatcc aaacgtcgag gtggtggtgg gttgccacgg
    24001 cttccccgca ccggcgcggg agtcgttccc cgagccggtg cttggggtgc tggggcccgt
    24061 cctcgagttc ggctcggaca tgcttttcgg cgacgtgctg gctggactct ccgcggccgc
    24121 gtccggtgac ttcatcacga aggtcgacga cgacgactgg tacggcccgc atcacctcac
    24181 cgacctgctg gccgcctgga cgtactccga ggcgcacctg gtgggcaaga agctggccct
    24241 ggtgcacttc gaggaggacg actcgctggt catccgccgg ttcttcctgg agggctaccg
    24301 ctggcaggtg gccggtggcg cggcgatcat cgccaagcac gacctgaccg cggtcggcgg
    24361 ctggcgctcg cagcggcgcg ctgtcgatcg gggcctgtgg acccgcatgg aggaggccgg
    24421 tgcgctcacc tacgcctgca gcggccccgg ctacgtgcac acccgccacg ccggcccaca
    24481 cacttgggcg gtcaacgacc agcacttccg cgagtcctac gccgaggaga cccttaccgg
    24541 cattcccccc gccgcgctcg gcgtccgctg acgctcagct cttgacgcgg aactcgttct
    24601 ggatggacgc ggcgtgcagc ttggcttcgg cggtgatccg gtccccttcg gccacgaatg
    24661 cggcgagcgg gccggcgggc aggtcgccca gcacgtactt cttcatcttc agccggtcgg
    24721 cgcgcagcga gtcgtccgcg agcgcgtccg caaggatctg cgaaaggttc gacaggtcgt
    24781 gctcgaggat gtacgacgcg cgctgggtcg ggaaggtgtt gtagtacgtc gccaggtcga
    24841 gttcgcgcgg gttggaggtg atcagcggcc gctcggtgta gaggtagtcg gtgaccacgc
    24901 tggagatgtc cgagatcagc gcgtccgcag catcgaagca ctcgtacaag gtgatggacg
    24961 cctccggacc gacatacagg tggccggcgt tcgccgccgc cagcaactcg acgatctcga
    25021 ccctggcctg agccatgccg ggacgctgca caccgctggc cgggtgcggc ttgaagatga
    25081 tccggacgtc gggcaactcc gcaaggatcc ggcggatcat ctgcgggccc atgcgctcca
    25141 gtgaggagta gttggactcc tcgtagtagc cctcgaaggt gggcgcgtac aggatcgtgc
    25201 ggcggtggtc ctccgcgatc gggccgaccc tcagctgctc gacctgcggg cgtccgatga
    25261 tcgcgtactg gctgtcgggc atgtggatgc cggccgcctc gtagcggtcg atcgcggcct
    25321 ggccggccac ccagacgccg tcgtagaccc gcgagaccgg gttcgccgag gtggacttgt
    25381 cggagtcgcc gtggttgagg aagacgtgcc ggatgctggc ctcgcgcagc aagtgcacgt
    25441 tcttgcctgc gttggccagg tagtaggcca ccatcgtggt cggccggatc agctcctcga
    25501 cgtgccgggt cttcggcgca tacaccaccg gcaccggcgt cggcgcgatc ggctgcaggt
    25561 ggctggcctc gcgcaccatg atgaacgact tctggtgcag cgcctcgatc gccggcaccc
    25621 actggttcag gatgtagagc gactggcccg cgtccgccga catgtagacg accgcctcgg
    25681 gagcgatcgc gtccaactcg gccctgacct gattcagcac ccgggccacc gatgcgtccg
    25741 agccgagcac gcgcagccgc tgaccgtgcc ggacgccgag tacgcccagt accacgacca
    25801 gcaccaggac gaagcccaca ttgagccaca actgccagcc aagcacgtgc gcaaccagcg
    25861 tccaggatgc gacgagaagc tcgagcacca ccaccagcgg gttctgccag gcggacgccc
    25921 ggtactcccg cgcgaaggcc acgctggccg cgggctgcgg ctcgccgttg gtgcggtagg
    25981 ccagcgcggg ctgacggccg ctgagccagg cggagcccgc ccgcagggtc aggtgcacca
    26041 tctggatcag cagcaccgcc gccgcaaccg cagcggccag ccagccgtcc ccgccacgtc
    26101 ccaccagggc gacgagcagg acgccgcgaa tgagctgccg gacgttcgcg cctacgccca
    26161 gccggcgcat caacagcgtc tcgcggacgc aggagcggga agccagccag tcggtgagta
    26221 cctcgagggc gaggaagatg atgccggcaa ccacccagcc gccgatgatg caggcgacga
    26281 cgatgcccca gatcgctccg gtgaccgcca ggtagtagcc ggacagcaac cggtcgagcc
    26341 agcccagcca cgccggcatc tgcggcgtgc cgagcacagc gcgagcgaac tcgaaggact
    26401 ggggcatccg caccacctgg gcggcatcag cggccttggg gtcctcagac tcgctctcga
    26461 cctgttcctc gtcactcatc agtgcatttc ccttctccgc cggcgcgggc gacgcccggg
    26521 ctgagcctgc cacccgcggc tgtgcaacgc ctcagcggcg agactcctcg taggcggtga
    26581 ccacttcgtc ggtgggaccg tccatgatca ggtcgccctt gttgagccag atcgtccgcg
    26641 agcaggtgtc ccggatcgag gtcatcgagt gcgagaccag aaacaccgtg ccggcgttgt
    26701 cgcggatctc gcggatgcgg ttctcgctgc gctggcggaa gcgccggtcg ccgaccgaca
    26761 gcgcctcgtc gacgatcagg atctcgtggt gcttgatggt cgcgatggcg aacttcagcc
    26821 gggcctgcat gccggacgag tacgtccgca tcggcaggtc gatgaactcg ctgagttcgg
    26881 cgaagttcac gatcgggtcg tgcagctcct cgacctcctt gcgggtgagg ccgagcgcca
    26941 agccgccgag catgatgttg cggtcgccgg agaggtcggg gatcagcgcc gcaccgacac
    27001 cgagcaggtt cggccgggac gctgcgtaca ccgcgccgga ttccggcggg gtcaggccgg
    27061 tgaccgcccg catcagggtg gacttgcccg aaccgttgga gccgatcaca ccgatcgact
    27121 cgttctcgta ggcgacgaag ctgaccccct tgagtgcgtg caccacccgg atgccgcggc
    27181 tgggcgcctt gaggacaccg cgggtgttgc gcttgatcgc cttgccgctg gagtagaccc
    27241 ggtacttcac gtgcaggtcg tcgacgatca cgaccggacg ccggccgtcc gcggccaccg
    27301 aatcgggggt gatcaccgga cgcagcgggg tgagtggctc agtcacgtcc gtacctctcc
    27361 tccgcggccc agaagaacag caggccgacg acgaaggtga ccaccgacca gatcgagaag
    27421 tacagccagt acatcgggtc gtagctgaac ccgcccatca ggctggagcg ggccatctgc
    27481 agcacctggt agagcgggta gaagtcgtag agctgcatca ccgcggggtg cgccttgaag
    27541 atcttgctga cgtcgaagag caccccggag gtgtagaaga ggatgcgcga aaggtacggc
    27601 agcagctggc tgaggtcctg aacatggacg gtcaaccggg ccgcgatcag cgccaccccg
    27661 gtgttgaaca gggtgaacag gaacagcagc gggatcatcc ccagccagct ccaggtcggc
    27721 ggatgcccca tgaacggcag cagcacgacc agcaggccca tcgacggcag cagcgaaagg
    27781 aactgctcga tcacctcggc caccggcagc gtcagccgcg ggaaggccag cgactgcacc
    27841 agcgactggt tgccggtgat cgacttggca ccctgggtga ggctcttggc aaagaactgc
    27901 cagaggaaca ccccgatcac gacccgggcg gcgtaatcct cgccacgggt cgcgctgctc
    27961 tgcagcacgc cgaagatgat gccgtagatg accgcgttca gcaccggctg cagcagcagc
    28021 cacaggatgc cgagccggtt gccttcgaga tcggcgcgca gccggtagcc ggccatcgct
    28081 gtgatgaagt cgcggcgctg ccaggcctgc ttgaggtagg caaagaacgg cggacgcgca
    28141 ccaacgcgat gcaggccgtg atcctcggcc agtgcggcca gactcatgcg cttcgcttct
    28201 ccatcgctac tcggccgggc tggcctgcgg acctgccgat tctatcggac gtgccgggca
    28261 atctcttcac caccgctccg gaggctcccg gaggcggctg cggacccacc ggccgtacgg
    28321 ccggaggccg tagcgtcagc ggcgtccacc cgggtcgcga aaacggagcc cgaagctatc
    28381 gcgccggctc aagcaggtga accaaccctc agcgggcgcg gtgggccagg tagtagccga
    28441 ccaggttctg cgtggacgga tcctgcgccg cgagcgcgtc cgcgtccccg gcaacggctg
    28501 gagcgatctg cagggcgagc tgcttgccca gctcgactcc ccactggtcg aagctgttga
    28561 tcccccagac cacgccctgc acgaacgtga tgtgctcgta gagggcgatc agctggccca
    28621 cgacggacgg ggtcagcgcc ggcgccatga tcgaagtggt cggacggttc ccggggaaca
    28681 cccgcgccgg cacgatcgcc tccgcggtgc cctcggcgcg cacctcgtcc gcggtcttgc
    28741 cgaaagccag cgccttggtc tgggcgaaga agttggcgag gaagagctcg tggacgtccg
    28801 cgtccccgtc cttggtggga tgggccggag tggcgacggc gatgaagtcc gccgggatca
    28861 gctgcgtgcc ctggtggatc agctggtaga aggcgtgctg gccgttggtg ccaggctcgc
    28921 cccagaagat ctcgccggtc tcggtggtca ctgcgctgcc gtcgaaccgg acgcccttgc
    28981 cgttcgactc catggtgagc tgctgcaggt acgcggcgaa gcggtgcagg tactgcgcgt
    29041 acggcagtac cgcgtggctg ccggccttga tgaagttcac gtaccagacg ttcagcaggc
    29101 ccatcagcgc cgggacgttg ctcgccagcg gggtctcgac gaagtgccgg tcgatcgcgt
    29161 tgaagccggc caggaagtca gcgaagtttt ccttgccgat cgccaccacc aacgcggtgc
    29221 ccacggcgga gtcgaccgag tagcggccgc cgacccagtc ccagaaaccg aaggcgttct
    29281 tggggtcgat accgaaagcc gccaccttgt cgagcgcggt ggagaccgcc acgaagtgct
    29341 tgctgatcgc gttgcggttc tgttcgtcgc tgccgtcgac ggcaccggct tcgagcaact
    29401 tggcgaggaa ccagtcgcgc gccatccggg cgttggtgag ggtttccaac gtcgtgaagg
    29461 tcttggacgc cacgatgaac agcgtggtcg ctggattgag gtccttgacc ttctcggcaa
    29521 cgtcgcttgg gtcgatgttc gagacgaacc ggcattccag gccgccctgg acgtacggct
    29581 ggagtgcctc gtagaccatc accgggccga ggtcggaacc gccgatgccg atgttcacca
    29641 cggtcttgat ctgcgagccg gtgacgccgc gccactcccc cgtccgcacc ttgtcggcga
    29701 acgcgtagac ctggtcgagc acctcgtgca cctgagcgac cacgtccgtg ccgtcgacca
    29761 ccaggctgtc ggtgcgcgga cggcgcaggg cggtgtgcag caccgagcgg ttctcggtga
    29821 cgttgatccg ctcgcccgcg aacatggcgt cgcgcttggt ctcaaggcca acctcctggc
    29881 cgagttcgac cagagcggag cggatctcgt cggtgatcag gttcttggag aggtcgacga
    29941 aaaggtcggc cgcggtgagg ctgaaccgcg ccgcgcgtcc gggatcttcg gcgaaccagc
    30001 cgggcagatc gggcttgaga tcggcggcca gctcggtgag cttcgcccag gcagcagtgg
    30061 tggtggcatc gacgggtgca ctcatgggct cagcctagtg aatcccagca ccggcaaggt
    30121 ggacaggctg cggaggcgtt gctagtcggc gcagacgcgg gcgacgttcg cggcgaacgc
    30181 agcttggggg ccgccgggcg ggtcgcccag gacgcgggcc ttcagttcag cccgtgccgg
    30241 acgcttgggg tccgcgccga acgcgtccgc cagtaggccg tccaggtcgg ccaggtccgg
    30301 ttcgaggacg tacgaaccga ccgacgtcgg gtacgtcgcc gcgaacgcgg accccccgag
    30361 gccagccggg tcacaggtga ggatcgggcg tccggtgtag aggaactcgg tggccacgct
    30421 ggacacgtcc gtgatcagca ggtcggcttc ggcaaggcat tccgggagcg tgagcgactg
    30481 gtcggccacc acgcgatgtc cgcccggcgc tgagcgcagc agcgtgttga tctggtcgat
    30541 cgcggccagc atcgacggtc gtgagacccc gctgctcgga tgcggccgga accacacccg
    30601 cacgtctccg cgagcgacca gccgccggat gagctcgacg cccatccggt ccagcgagct
    30661 gtgcgcggtc tggtcgtaat agccctcgaa ggtgggcgcg tacaagacca cgggagtctc
    30721 gtgcccggtg ggtccggacg gcagcccggc ggcctgcggg cgtccgatga accggaagcg
    30781 ctccgctggc aggtcgaccc cggcggcgcg gtagcgctcg accgccatcg gtccggcaac
    30841 ccagatctcg tcgaacgcgc gagccatgcc gttggcgctg gtggccttgt cggagtcgcc
    30901 gtgcagcagc atcacgtgct tgagccgcgg ttcgcgcaac agttgtccgt tgcgctcgcc
    30961 gtaggccagg tagaaggccg cgcggacgcc ggccagggtg acccgctcca cgtcccgctg
    31021 gctgggcgcg tagatcaccg gcagccgggt ggcgccgatc ggcagcagct ggctggcctc
    31081 ccggaccagc aggaacccgg gctcggccag ggcgtccagc gccggcagcc actggttcac
    31141 gatgtacttc gattggcggg cggccagcga cacatagacc acgaaccgcg gatcaatggc
    31201 acccagttcg gcgagcacct gctcgcccca accaggctgc tgagcgcgga cggatcgggc
    31261 ggccagcccc gtccagagca ggtagccgat cgccaccagc gccagcgccc cggcgatact
    31321 caggcccaac cagaccggca tcatcccgta gccgagagca acggccttgg cgagggcgac
    31381 gaactcgatc gccgccagcg cgaatggcgt cccgacggcg cgggcataca cccgggcgtg
    31441 cgccgtgaac gcgtccgcct cgtgaacgga aaaggaactg tcccctcctt gttccgcctg
    31501 aacggaaagg ggacgggtcc ttttccgttc agccgagtag cgcaggggcg gcgcgcttcg
    31561 ggagacccag gtggccagga cgggctgcac cgcccaggcg agctgcgcca gcaacaccac
    31621 cgtgacgtag gccgaaccgg cgaccacgcc gttgggggtg gtcaggaaga gcagcccggc
    31681 ggcgggcagg ctgcgcagca gcgcgcgtag cggagcccgc aggcccacca gatcgagcag
    31741 ccggaccacg ggactgtccg tgccgctggc cagccactcg accagcgcgc ccagcgtcca
    31801 cagcaccaga cccagccagg gcagccgcag ccacagggcg agcagcagaa cgatcgtgca
    31861 cagcgccacg accggaagga aagcctgtgc cgccatcacg ctgacccgga cgggcacgcg
    31921 gccgggccag gcggcgcggg cgacttcttc ggtttcggga acgggcgccc gtccgagatt
    31981 gcccaggacg ccgccgatgt tcacccgatc agcgtagtgg gcgggctcag ttcccggcgc
    32041 gccggcgccg gcccgagcgc atcagcgcgc cgatgccgaa gaaggccagc acgctgccga
    32101 gcaggccacc accggcgacc agcgcgatct gcacccagcg gtcgccctca agctcggcag
    32161 ccgtccggac gcgcggcgac gggctcggcg tggccgtggc gaccggcgtg ggcgtggggg
    32221 tggccttcgg cttcttcttc gccttctttg tcttggtggc ctccggctgc ggcatcgcgg
    32281 ccacaccggt cgggacggcc gccaacgccg gcgtcaccag ggtcgacagg cccaccatgc
    32341 aggccacggt cagcaccatg accaggaatg cgcgcatcgc ccctccgtcc gatccgaaca
    32401 ctttagcgtc ggacggaggt acgcagcagt cctcacgacg gccgtgcccg cgcttccgtc
    32461 cacatccgct caaggggttg aatcgcccga ttccttaagt tatcctgaga aacgcctcag
    32521 aacgttcaga acgcaaggac taccgcatgt accagccccg acggatcgct gatccgatcg
    32581 agccggcgct cggcgtcccc gcttccgggg cagccaattt cgactcccca accggtgggc
    32641 cacggcgggc actgccggca tcgaccgacc actggtatcg ccgcgggagc gtggcgctgg
    32701 ctgtggtgac caccgctgcg accgcaggcg cggtgatcct gagcaacacc agcacgaccg
    32761 cggcggccct cggcggcccg gtgctcgtcg agcagcctgt gctcgcggcc gcgatcccgg
    32821 ccgcaccgat ggcgaaactg gtcgccgaag agttggccgc ccccaagctg gtcggttcgg
    32881 ccaagcaggc cgacaccgcg atgctgagcc tcaccaagct gtactcgtcc ggttcgctga
    32941 acgtgcgcgc aaaggccaac tcgaagtcga agctgctggg caaggtgacg accgctgcca
    33001 ccgtcaaggc caccaccgag atcgacggcg actaccggaa ggtcgagttc gaggacggct
    33061 acggctgggt gctggccgac gaactcaccg acaactcgcc cgacggcgtc ttcatcgacg
    33121 acggcaccgt gcccgagggc accacgatgt cagcgtgttc gcgcggctcg ggcgttgagg
    33181 gcaagctccg caaggacacc gtccacatct accggtcggt gtgcgcgctc ttccccgcgg
    33241 tgaactccta cggcggctgg cgcgccggcg gccgccagtt ccacaagaac ggacgcgcgc
    33301 tcgacatcat gctcaccccg caaaaagaga gtgcgctggg caagcagatc gcggcctatc
    33361 tgatcaagca cgccaaggac ttcaacatcg accacatcat cttcgagcag aagatctgga
    33421 cgcccagcac tccccgctgg cgccacatgg ccgatcgcgg cggcatcacc gccaaccatt
    33481 acgaccacgt ccacgtggcc attcgcggct gagtagcccc aaacgcactt agcccggatc
    33541 caggcttagg cgcggcgcca aggtcagtcg cccaagcgcc acttctgcag cttgctccgc
    33601 aacccgtcca ggctctcggc gagcaccaac tcggggctca gcagatcctg cggccatacc
    33661 ccgagcgcgc ccgcgtccat ccagatgtcg gcctggtagc gctccatgcg tccgttggcg
    33721 atcagcgtgc cgggcggcag catcggcggc aacgagcgtg caaaacccgt cccgaaggcg
    33781 tccgtcggta gcagctggac ggccgccgca cccgcgtcca gtcccgccag cagctcggac
    33841 ggggtcatcc cgccgagaat caccggcaag cccggcaccg cgtccaccag ttgcggacgc
    33901 aggaacggag cagcgacgaa cgcagccccg gccgcggccg ccgtccgcag ctgttcgggt
    33961 tcgctgaccc cgtggacgcc cagcttcgcg cggcggccga aacggcgctg caactccggg
    34021 aaatcgtcca ggcgctcgaa caccaccgac cacacgcgga agccctcctg ccagagcacc
    34081 tcggccgccg gcacgagatc aggcaggtcg aagtcgggca gcgaaacgat cagcgagtgc
    34141 gcgcccagcc cgggcatctt cgaggccacc ggtacatcct gcctgatagg cgctgacatc
    34201 cgcgcggacg cgtggttagc tgggtgcgtt ccaagccgtt tccgaccagg caacacaggg
    34261 ggaccgatgg agttcaccag cgagaacatc gctgccggaa gcagcatcga cctgcgctac
    34321 gccgagcccg gcgtgggcgg cgacaacgtt tcgccccagc tcacatggtc ggccggcccg
    34381 gacggcacgc agagctacgc gctcacctgc ttcgacccgg acgcgcccac cggcagcggc
    34441 tggtggcact gggtggtcac cgacatcccc gcggaggtga cctcgcttgc cgagggcgcg
    34501 agcctgcccg ccggtgcgcg cacctggacc aacgactacg gctaccccgg ctggggcggg
    34561 ccgtggccgc cgccgggccc ggagcaccac tacgtgttca ccatcttcgc cgtcggcgtg
    34621 cccaggctcg acgtgcccga cgacgcgtcc agcgccgtgg cccggttgac gctgagcttc
    34681 aacgtgctgg agagcggttc gttcacggcc gtcttcgcca acccggccag ctgaggttca
    34741 ccgcaggtgg cggtgctgat cgatccgccg cgatggccgg cgcacgggac ccggttcgcc
    34801 cacctggtct cggacgcgac cctggatgag ctcttcgagt tcgtcgacgc cgcgcaggtg
    34861 ccgccgcgcg ctttcgacca tgaccactac gacgtgccgg aacgccggta tgccgacctg
    34921 gtctcctccg gtgctgagcc ggtggacgag aagaccctgt tgcggcggct gaaggacagc
    34981 gggctgcggg tgcgtccgcg cgatcggacg ccgcgcaccg cccaggtgct gccggcgcta
    35041 gagcgggcgt ggacggagtt gctgccagag caggtgtcgc tcggggcgga gctgctggaa
    35101 agatggcagg agccgaaccg gcactaccac gacgtgcgcc acctgggaca ggccttgagc
    35161 gcgcttgccg aactggactc gcggcagtcg cggacggccc ggctggcgtt gtggttccac
    35221 gacgccgtct acaccggcag cgccggagca gacgaggatg cgtccgcgga gctggctgta
    35281 tcttccctag ccaaggtggg tttgccgacg gcgactgtgg atgaggtagc ccggctggtg
    35341 cggttgacca gcacgcacac caccgacgac ggcgacgacc gcggcgccct ggtgctggac
    35401 gcggacctgt cggtgctcgg caccgcgccc ggccgctacc acgtctactc ccgcgacgtc
    35461 cgcctggagt acccgcagtt ctccgacgcc gagttcgcat cgggtcggct gcgggtgctc
    35521 gaccggctgc tcaacctgga caccctctac cgcacgcccg tcgcccagca gcgctggctg
    35581 gcgtccgcca gatcgaacct ggcgagcgaa caccgtcgct gggcccgcct cgctgcctga
    35641 agactcggcg aggctgtccc tggatcacga cccatcgcag gatcaaccca cttttggcag
    35701 gttctgaggg agtcgcgacg cagccaatcc ggaccaaccc agaaactgct ggttatgcgg
    35761 gctgaaatcg ccttcatagc cagcattaat tgggttggtc gctgtgagag gtgcgatcgg
    35821 gccgccataa cctgacggaa ctgggttgaa ccggggcgag ggcgagcgga gacccgctgg
    35881 ccggtttcgg cccggctctg gtcgccgcgt accctcgttg gttgtgagcg gagtatccaa
    35941 agccctcttg atcggtgccg tggccagcgc gctggcgttg ggcggctgcg cggtggcacc
    36001 gcccgaggcg agcctggctc ccagctcgac agctgccagc agcacgaacc ccccagccgc
    36061 taccccaggt acgcccacct cggcaccatc agccacatca gcgacaccgt ccgaatcggc
    36121 cagccccgcg accgcggctg ccaagccctc ggcagccgcg aagccgtcgg cgcgcgccaa
    36181 gccggcaccc gccaagccgg cacctgacca gcgcaagggt aagcaactca acacccccta
    36241 cgaagtcgac ggcatcgtcg tcgtctccag gcagcaccgg gtttcagcgg cctacaagcc
    36301 gaagaacccc accggaccct acggcctgac caaagagacc gcggcggccc tgaagaagat
    36361 gaccaaggcc gccaaggcgg acggcgtccg catcgtcgtg cgcagcggct accgcagcta
    36421 cgccacccag aagggcgtct accagcgggc gctgaagaac tatccgagcg tcgcttccgc
    36481 caagcgctac aacgcgccac cgggcgccag cgagcaccag accggccttt cggtcgacct
    36541 ctgggacggc gtcacctgga gcctcccgat ggccaacacc gccaccggaa agtggctgca
    36601 caagcatgtt cacgagtacg gcttcgtgct gcgctacccg aatggcaagg agaagatcac
    36661 cggctacgcc tacgagccgt ggcatttccg ctacatcggc gtcgctgatg cgaagaagtt
    36721 cggcaaaggc aacacgctca ccctcgagga gtacctgggc ctcgcctgat ccgcaccaaa
    36781 gatccgtggc gttcgatcac ggcgactggc tcttcacggg agaatgactc ccatgacggc
    36841 aaacctaccc gacaccgcgg gcaccgagaa gatcggcgcc ggcaagcgca tcggcatcct
    36901 caccagtggc ggtgacgctc agggaatgaa cgcagcggtg cgcgcggtcg tccgcaccgc
    36961 aaagacgctc ggcgcccgcg tctacgcgat ctacgagggc taccagggaa tggtggacgg
    37021 cggcaccggc atcagcgagc tcgcttggga ggacgtgggc aacatcctcg gcaagggcgg
    37081 gacgatcatc ggcaccttcc gctccaagga cttccgtgag tactccggac gccttcgcgc
    37141 cgcggccaac ctgctggaac acggcatcga ccgcctgatc gtgatcggcg gagacggttc
    37201 gctttccggc ctcgaccagt tccgggccga gtggacgcaa ctgctggccg acctcgtcgc
    37261 gtccggtgag atcaccaagg agaccgcgga cgcgcaccct tcgctgatgt tcgcgggcct
    37321 ggtcggatcg atcgacaacg atctggtcgg caccgacatg accatcggcg ccgacaccgc
    37381 cctgcaccgg attgtggacg cgatcgacgc cctgtccagc accgcggcca gccaccagcg
    37441 ctcgttcgtg gtcgaagtga tgggtcggca ttgcggctat ctcggcctga tgagcgcgat
    37501 cgccggcggc tgtgactacg tattgatccc cgagcaaccc ccggaggacg gctgggagga
    37561 gcgcatgtgc cactccctgc gccgcggacg ggacggtggc cggcgcgact cgatggtggt
    37621 cgtggccgaa ggagcgtgtg accggcacgg gcagccgatc acgtccgagt acgtgcgcaa
    37681 ggtgatcgag gagcacctgc aggaggacgc ccgggtgacg atcctcggcc atgtgcagcg
    37741 cggcggcgca ccgagcgcat acgaccgctg ggcgtccacc tggttgggct acgaggcggt
    37801 gcgcgaggtg ctgtcggccg agcccggaac gaccggtccg gtgatcggtt tccggggcaa
    37861 tcgggttacc cgcgtgccgc tggtggaggc agtcgcgaac acccggaagg tgcccgaact
    37921 gatcacgtct ggcgactacg cgggtgcgat ggcgggacgc ggcggctcgt tcaccgaagc
    37981 cgcgcagatc ttcgccgagc tggtcgagcc cagccgggtg gacgcgccgg aggagtccaa
    38041 gcggatcgcg atcatccacg ccggcgggct ggcgccgggc atgaacgccg ccgcgcggga
    38101 cgccgtccgc ctcggcatca gccgcggcca caccatgctg ggcgtgcgca acggcttccc
    38161 cgggctgcgg gacggggaca tcggcgaaat cgaatggggc gacgtcgaga actggctcac
    38221 cgacggcggg gccgagttgg gcctgcggcg caccgttccc ggcctggacg agctgtaccg
    38281 gatcagccgc tcgatcgagg atcaccgcat cgacgcgctg ctgttgatcg gcggctggaa
    38341 cggctatgag gcggccgagt tgatgcaccg cgagcgcgac cggttcccgg cttgtgggct
    38401 gccgatcgtg tgcgtgccgg cgtcaatcga caacaacctg ccgggctcgg agctgtcgat
    38461 cggcgccgac accgcgctga acgtgatcac cgaggcgatc gaccggatca agatgtcggg
    38521 cagcgcggcc aagcgggcct tcgtggtgga gacgatgggc cgctggtgcg ggtatctggc
    38581 cttgatgggc ggcctttccg gcggcgcaga gcgggtctac ctgcacgagg aggggatcag
    38641 cctagacgac ctttcgtccg acctggactg gttgcgcaac agcttcgcgt ccggacggaa
    38701 gctctttctc gccgtccgca atgagaacgc gaacgcgctc tacaccacgg atttcctcgc
    38761 ccggctgctg gagcaggagg gccgcgggct ctacgacgta cgccagaacg tgctcggcca
    38821 catccagcag ggcggctgcc cgtccccgtt cgaccggctg ctggctgtgc gcctggtctc
    38881 gcgagcgctg gacgtgatca gcgccgaatt caccgccggg acgcgaaaca gctggtacct
    38941 gggcctggtg cagtcaaagg tcaccagttt cccgatgacg cacatgatgg acgagatgga
    39001 caagcgctac aaccgtccga aggagcagtg gtggctcgcc ctgcgtccag tgctgaaagc
    39061 ggtcgcggac gcggggacgt aggttctcgc cgagctcgac cgaggtagtt ccgaactcag
    39121 cccgcgaacg tcgcagtgtc gatcacgaag cggtagcgga cgtcggaggc gaccacccgg
    39181 tcgtacgcgg cgttgatgtc cgcacccgtt atcacctcga cttgggctgc caggccgtgc
    39241 tcggcgcaga agtccagcat ctcctgggtc tcggcgatgc cgccgatctg cgaaccggcg
    39301 aggacacggc gctggccgac caggccgccg aggttcagtt ccaccgggtg ctcgggcaga
    39361 ccgacgtcca ccagcgcgcc gccggtcttc aggcagttga tcaggccgtt caggtcctga
    39421 tccgctgaga ccgtggacac gatcaggtcg aactgcttgc gcagactgcg cagcgtcccc
    39481 ggctcggaga ccgcgtagta ctccgtagca ccaaagcggc gtccgtctga ctccttcgag
    39541 cgggtctggc tgatcacggc aacctcggca cccatcgcag ccgcgatctg cacgcccatg
    39601 tggccgaggc cgcccatgcc gacgatcgcg acccgcttgc cgggaccggc gccccagcgc
    39661 ttgagcgggg agtacagcgt gatgccggca cacagcaggg gcgcggcctg ctgcaggccg
    39721 atcgagtccg ggatgcggca gatgtagtcc tgctcgaccg tgatgcacgt tgagtatccg
    39781 ccagcggtgg gcagaccgtc gcgtccggtg ccgttgtagg tccagatcgc gccgcccggg
    39841 ccatcgcaga actgctcgtc gccggcccgg cattgctcgc actcgccgca ggaatcgacg
    39901 aagcaaccca cgcccacgcg gtcgccgggc gcgaacttgg tgacttcgct gccaacctcg
    39961 cgcacgacgc cggccagctc gtgacccggc accagcgggt agttgacgcg tccccattcg
    40021 ccgcgtgcgg tgtggatgtc ggagtggcag atgcccgcgt acacgatgtc gaacagcact
    40081 tcggtcgggc cgggctcgcg gcgggtgatc gtggttcgcg cgaaccgtcc ggtcggtccg
    40141 tcgatggcgt aggcagtggc ttcaggcatg tgcgctcctt cgattggtct cggccccatt
    40201 caacacccgc gctaagagcc gatccagccg gatcatgcac caccccgttt ccccaaagtg
    40261 gaccgtttcg cgctaagacg ccgacagacc gtccactctg cgaggagctt ggggccagcc
    40321 aatcgagcta gagcgacccg agatagacac ccagcagggc cgtcagcagg ctgagcacga
    40381 gcatgccgcc ggcgtggacg agggtcgtcc agccccgtcc gtcgagcgcg agccgagccg
    40441 cctccaccga cgcggtgctg aaagtcgtat agccaccaag gaaccccgtg ccgagcactg
    40501 cccgcaggcc cgggtcgccg ctgtggctca tccaccatcc ggtgatcacg ccgagcagca
    40561 gcgagccggt gacgttgatc aggacggtcc caagcggcat cccgactcgc agtcgagccg
    40621 cgatctggcg atcgaccagg aagcggacga ccgctccgag tccacccgcc aacgcggtca
    40681 gcagcgggat catgccgcct ccccgagcag tcgcgagaac actgtcctgc ccaagcgcag
    40741 gccagcgatc gcagtcagga tcccgaacac cacgctgacc agggcatagg cagccccgac
    40801 caaccactga cccccaccca gtcgctcaac cgactcgacc gcgaaagtgc tgtaggtggt
    40861 gaacccgccc atcactcccg tcccaacgcc aagccggacg ccacgccgcc agcccgcgtc
    40921 cgaaccgctg atcgcgagtc cttcgagcag cagcccaagc atcagggagc cgccgatgtt
    40981 gatccagaag gtggtccacg gccaggcgcc aggttgcgcc ggccaggtgc tctccaacca
    41041 cgcgcggacc gctgtgccga gcgtgccgcc gagcaggatc aggagcagcc agcgccagtc
    41101 gggggatccc ggcctacgcc gggatgacga aggagtgtgt ggccgggatg acgaaggagt
    41161 cactgcacct ggtgcttcca gtcgacgacc tccaccggca ccgccagcac cggacgatgt
    41221 tgatgccggg tcaactgtgc ccccagcgac gcggccagga actcacgaag ccgctcgcca
    41281 ggtaccggac gccgagcgcc caccacgatc gcggacgcgt ccagcgcacg ggccagatgg
    41341 gtgacggcac ggtcggcgcg tccggccaga taatgcagct cccagtggac ctcgtgtccg
    41401 gtgagcgcgg acgtgatctg cgcgcgcaac tgctcctggc gctccagcca ggagtcgtcg
    41461 tctgagtctg gtgacaaggc ggaatgccgc accgaaccat ccgcatactc cttgtcgagc
    41521 acccgctcgg gatcgacata cgcgaagtgc agggttccac cgacggccgt tgcccaggtg
    41581 gcggccgtga cggcgacgat cggcgactgc ccgggcacca ccccgacaac cagcgcatgg
    41641 gcggtggcgg gcacgatgcg gtcgacggcc ggttggggcg ccgactcggt gaacggcctg
    41701 ccgggaggtt tggtggacat cccgacctca cgatccgggc gcgtgcgccc aacgacgtgc
    41761 aggaaccatc agccggtcgg cggttcggga cgtacccggg cgggatccat cacccagcag
    41821 cgaccctagc ccaccagctg acgccagccc agtgacgatt tgcaccgtcg atcagtcaaa
    41881 tcggccaaat caggtccatc aggatcatcg actgggcaaa ttgtccgcga gtgacagtcg
    41941 gttgacctcg ctgatcccgg caccatcgga cggggacacg ctcaccaggt cggcgatgcg
    42001 cgacgatgtg tcgctgcggg cgccaagcgg gccgaacgct tcgatcaggc cggcgtcgat
    42061 aaagatgcgg acctcgccgc tcgactcagg ttcgacgagc tcgccaatcg ggatcatcag
    42121 ctcagcctcg gggccttcga ggatcagggt gcccgtcgac ggtgtgattg cgacgatttc
    42181 cagttgagtc ggcgagttcc ccgccctcaa tccatcgtca tcccggcgca ggccgggatc
    42241 tccgagcgcg atcgcccgat ctgatcccag gtgtcccctg ccgaggggcg tgcgcaccaa
    42301 gtcatccagt tcggctgcgg gacggagcac tactcggtca cccaccagag tcagttcgcg
    42361 ggggcagctc atcgcgccga cccaggtgct gacgccacgg tccggatcgt cctgggtgcg
    42421 cagccagccg agctgcagcc agcggccgtc cgcgtgctgc cacggcagcg ccgcgtagta
    42481 gcgagggccg gcgtcgaggc gaccgaccca gagtggacgc gcctgttggc cgggctcgcg
    42541 atcgagggtg actgcccaga ccgacaaccg ttcggcggcg ccctcacctt cgatcaccga
    42601 cagcatcgcc acccagcgcc cgtccagctt gatcaccgac gggcactccc acagtcgtcc
    42661 ggggacgccg ggaagcgcgt cccgaccgag cgcgaaggcg tactcccagt cggcgccggg
    42721 cgaggattcg cgctcggtgt agaccagcag tgacggctgt ccatccggca gcaccgcggc
    42781 gagaatcagg tgccgctgcc cctcgtgcca ccagacgaag ggatcgcgga agtcgtggat
    42841 tgccgggtcg ggcggggtcg caaccagcgc tccgggaacg agcgagtcca ggcccgggtc
    42901 ggtggtgcgg gcgacccgcg gacgctgctt ctgcgctccg tccgacaccg cggtgtaaaa
    42961 caactccacc tcgccgtgag cagtcagcgc ggcgccgccg ctccagcagc ccgtcccgtc
    43021 cagagcgtcg gtcgcgccgg ggaccagcgc tactggacgg tgttcccagc gcagcagatc
    43081 ggaactcacc gcgtggcccc agtgcatccg tccgaacgcg gtggcgaccg ggttccactg
    43141 gtagaagacg tggtggctcc cctgccaatg gatcagtccg ttcggatcgc cgaggaagcc
    43201 agccggaggc gtcaagtgaa acagcggacg ggtcacccgc agcaccctag aggttcaggc
    43261 gcgttcggcg agagatctcg atgcttcgac aggattcagg gtggcgcttc gtcaagctca
    43321 gggaccgtgg ggcccttcgt caagctcagg gaccgtccgt ccgctcagct ctggctgttg
    43381 gcgtacgggc cggggttgtc cgccaaggtg tcctgacacg cctgcgaaca gaagcggtag
    43441 gtctgatcgc cgttcactga ggtgaaatcc acgccgtctt cgatcggcat gccgcaaacc
    43501 gcgcaggtgt cctggccggg agtcatgatg tcaacctttc gttgtcggtg ctccagcacg
    43561 tacccgcccg gccggggtgc aaccgcgctg ggcgtttgcg attcgctcgg acgggtaccc
    43621 ggacctcaga accaaagcga ttaggaggac cagatgtccg acagcgtcaa cgactacggc
    43681 ttgggcgcac ccaacaccca gcaggacgcg actcctgagc cgacccggca gtacgaggag
    43741 cccaacccgc tcgtccacgg ccttggcgac caggaccttc ccgacgacga tcacagcgag
    43801 gccggccggc ggcaccgttc gcaccaggaa cacggcctcg gcnnnnnnnn nnnnnnnnnn
    43861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    43921 nnnnnnnnnn nnnnnnnnnn nngaacacgg cctcggctcg ccggactcgg cgccggagga
    43981 cttcacccgc tgacctcgcc ctagtcgggc gaacgatccc tgagcctgac gaagggccgc
    44041 cgcacctgtt tcgatgatga ctgacgccct tcgtgacacg cgcctcgcaa gctcggccga
    44101 attcctcagg gaccgttgtg agggagaaca gacgtccttg cgatggacga atcggaccgg
    44161 ccggacctcg atccggccgg gccttctcgc gtcctcgacg gtcagctctg tactggcagc
    44221 agcacggctc ccgcgtgcgg cgggagcacc acgccaccat cggcgacgcg agcgccggac
    44281 gggcttgccc agcgcaactg gtgtcgtccg ctcagttcga tcgacacctc ttcgctgccg
    44341 aagttcaccg ctacgaccac cccgccgcga cgcatcacga accagcgctg cacgtcgtca
    44401 taggcacagt gcgtgccggt catcgctgga tcgctgagtt cgggcagctc gcgccgcagg
    44461 gcgatcaggt cacgatgcac gtcaagcagg agggcgtgcc gaccgtccgc caactcactc
    44521 cacgcaagct tcgccgcggc gaacgtctgt ggctcctgcg ggttgagcat ggtctccaga
    44581 tcccagttca ttcgcgcgag ctcggccatg cggcccgacc ggatcgcctc cgccaactcc
    44641 ggttccggat ggctggagaa gaatgggaag ggcgaactcg ccgcccactc ctcaccctgg
    44701 aacagcatcg gcgtatttgc cgtagtcagc gtcaacaacg ccgcgcaggc cagctggtcg
    44761 tcatccaggg acgcggccag ccgatcgccc gcagcccggt tcccgatctg gtcgtggttg
    44821 gagtagcaga tcaccagccg ccagccggga accgtccgcg cgtccagctg cggcgggcct
    44881 tcgctcccgc cgcccggcac agccacccgg tcgcgcacga acccacgctc gcacgctgcc
    44941 gccagcgcac cgagcgaggt gaactcggcg tagtagccct cggcctcccc ggtcagcgca
    45001 acgtgcagac cgtggtggaa gtcgtcgctc cattgcgcgt cgatgccaag cccaccactc
    45061 tgtcggggcg tgaccaggcc agggtcgttc tggtcggatt cggcgatcag cgtgagcggt
    45121 cgccgcaggt gcgcggacgc ggccgcggtc accaccgcga ggtcctccag caggtgtacc
    45181 ggcgacgagt ccaccagcgc gtggaccgcg tccagccgca gggcgtccac gtgcatgtcg
    45241 gtgaaccagt agcgcgcgtt gtcgaggatg tagttgcgca cctccgcgct gccctcgccg
    45301 tccaggttca ccaccgaacc ccacggcgaa tcctggtcgg tcaggtatgg gccgtacgcc
    45361 ggcagatagt ttccggaagg gccgaagtgg ttgtagacca agtcctggat cacggccaga
    45421 ccgagcccgt gggccgcgtc cacgaagcgc cggtaggcgt ccgggccgcc gtagggctcg
    45481 tgcacggcga accagcagac gccgtcgtag ccccagccgt gctcgccgtt gaaggcgttg
    45541 accggcatga gttcgacgaa gtcaatcccc agctccacca agtgcggcag ccggctgatc
    45601 gcggcgtcaa gcgtgccctc gggcgtgaac gtgcccacat gcagctcgta gatcacgccg
    45661 ccggcgagct ggcgtccggt ccaggcttgg tcgtgccagg tgaaaccggt ggcgtcgaaa
    45721 gtgcgcgaca acccgtgcac gccgtgcggc tgccagcgcg atcgcggatc gggcagcgca
    45781 gctttccgat catcgaccag gtagccgtag tcgattcggc ccagcggttc gggctcgacc
    45841 gggtgccacc agccgtccgc tgccttggcc atcgcgacgg tccgctcccc cgcaagaagg
    45901 cggacgcgtt cggctctcgg cgcccagacg tcaaagcgtc cacgctcacc cggacgccga
    45961 acctcgggca ccagcagcgc aaccggatac tgnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    46021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    46081 nnnnnnnnnn nntccggacg ctcccggcgc agggtgagcg cctggtggca gagcaacagt
    46141 ttcaccgcgg cgccgtccag cccggacggc agtgggggtc gcgccccggt gcgcaccttc
    46201 gccagcagcg cgacccgggc cgcgaagtcg accgggcggc ggttgtcggg gtccaccaga
    46261 ctgagttccc ataattcact gccctgatac aggtcgggca cacccggaac ggtcagatcg
    46321 aggagcttgg cggaaagggc gttgctccat ccggcggccg agatcgcgtc caccaccccc
    46381 tgcaggaccg tgcgcacttt cgccgaatcg cgcgtcgcgt ccactgcttc gaggacgacc
    46441 gcctcgtagg cgtggtcggg ggcggtccag gacgtgtggt cgccggcttc gcgcatcgcc
    46501 ttttcggcat agccgatcag ccgctcgcgc tcggccggcc acgccccgac gatggcctgc
    46561 cagagcaggt tgccgaaccc cggatcgggc aggggcacca gttcgagcag ctggctcagc
    46621 gcgtccgccc ataggtcggg cacttcggac aggacgctga tgcgggcacg gacgtcctcg
    46681 ccgcgcttgg tgtcatgggt ggtgccggcg gtcatcgcgt tcggccagac ggcctggcgc
    46741 tggttcatca ggaggtggaa gtcgtcggtg ccgatcgaga agatgctcgg atcgccaccc
    46801 acctcgttca gcgaggtcag ccgcgaccag cggtagaagg cgccgtcctc gacgcccttg
    46861 gccatcacca tgcccgaggt ctgttggaac cgctgcgccg gcccggacgc gccgtcggcc
    46921 agcaccggat gcagcagcgc gaagaccggc ccgaggtcgg gtcggtgcag ccgggcgtac
    46981 tcgaacgccc gctccaggtg gtcccggccc tcgggcaggt aggagcggta aacaccgaag
    47041 tcagcgagca attcggcgac cgcgtcggcc agcgcgtcgg ggtcgggctg ccagccggac
    47101 gggtcgatca ccgccagtac ctcacgggcg atccgacgca cctcagagtt caggatgcct
    47161 tcggccactg cgagcttgcc ctggtggacg agctcagccc agtcgacgtc gcgtccgcga
    47221 agccggttct caagggtggt caactgcact tcgcctgcgg ggtcgatcag tacccggtcg
    47281 atgatcgcca tcgcgtcgta gccggtcgtg ccggccgtcg cccaagtggg aggcaattcc
    47341 tcaagtcgga cgcttccccc ctcgcccgcc gcgtgcaggc cgaggatctt ctccaccagc
    47401 acgtaggcgc cgccggtgag cgcggcgagg tcgtcgaggt acttgccggg gtcgcgcagc
    47461 ccgtccggat ggtcgacgcg caggccgtcc accaggcctt cgtcgaacca gcgctggatc
    47521 tcggcgtggg accggtggag ccattccggg tcctcgaccc ggatggcgac cagggtgttc
    47581 accgcgaaga agcgccgata gttgaggctg cggtccgctt cgcgccagct caccagctcg
    47641 tagtgctggc gggcgtggac ggcgttcggg tcctcgtccg cgccgtgggc agtgccgggt
    47701 gcgagcggaa accgctggtc gtggtaatgc aattcgccac cgagcacctg gagatggtcg
    47761 atccgaccgt ccgcacatag gtcgtcgtcg ccgacgaccg ggagccgcag ccggccgccg
    47821 ccggccgccc agtcgatgtc gaaggcaccg gcgtagggcg agtcggggcc gttggtgagc
    47881 acctgccacc accactcgtt ctcccagggc tgggcgatgc cgacgtgatt ggggacgatg
    47941 tcgaccagca cccccatgcc cagccgcttg gcctcgctgg ccagcgccgc gaggccggac
    48001 gagcgtccgc gggctgggtc gatcgcgcgg tgatcggcga cgtcgtagcc gtgctcgcta
    48061 cccgattcgg acgccagcag cggcgagaga tagacccagt cgacgcccaa ctcgtggagg
    48121 taggacagcg tgcgcgcagc agccacgagg tcgaagtcct cggtgatctg caggcggtag
    48181 gtgctggcgg ggacgggtcg cacaaagtct cctcaagcta ggttcgtcac gctaggttcg
    48241 caggcggctt acccaatagc accgtatgca acccggctga caacgacaac gacgggcctc
    48301 gtgcgagcca acgccatgtc ctcgtcgccg cggtcaccgc acgatgcgca ggctgaactt
    48361 cgccgtgccg agagccccca tgagccggag ccagagcggc agccacatct ctacgccacg
    48421 ggcagcggtg atgtcgccga ggtcgatcac gtcccggtgc ctgagctggg ccaagagggc
    48481 gatcacgacg cgcttcgcgt ccgcgtcgtt gccggccacg aagactgtgg gctcaccgtc
    48541 cgcgagttct tcggggtggg ccatcaggtc ggcgttgagc gtgttcagcg tcttcaccac
    48601 tcgtgtttcg gggaaggccg actgaagctg ctcgccgagg ctgtcggtgt ccttgacgaa
    48661 gaggggacgg gggggacccg gccgagaagt caagcggatt tgagacatcg atcaacacct
    48721 tgccggccag gtgctcggcg cccaccgcgt ccagcaaagt cgcgcgacag cggccgcgtc
    48781 cggcatcggc cgaattgctt ccccggatgg tcgaagcccc gtctagcgga ccacggccac
    48841 cggacactcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    48901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng tgcaccaccc
    48961 ggagccgggc attgcgcagc gcggcctcct gcagcgccca gtccaacgca gagcggtgca
    49021 gcggccagga actcaccgcg agcgcgatcg tccccaggcc cccggtctgc tgcggggtgg
    49081 ccgcagcgga gatcacgatc accgggcagt tggcgcgtcc gaccacggaa acgctggttg
    49141 agccgacgaa catgcgttcc aggccgctga tcgagcggcg tccgaccacc agcagccggg
    49201 ccttgtcgct caagtcagcc agcacgtctc ccggacggcc gatcagcacc tcggaaatca
    49261 cccgatcgga cggaaaaccg tccgcttcga gctggtccgc agctactttc agcttgtccg
    49321 ccccggcctg ccgaagcacg tcgatggtgg acagcaggtc ccacagaccg gtgaccatcg
    49381 cctggtcgtc gaccacattc accagccaga ggtccgaccc gctcgcgttg gcctctcgca
    49441 tcgcgtactt gacggcccgc aggccgtccg ccgagccgtc cacgcccacc agcacccggg
    49501 cgtgtgcggc cccaatgggc tcgctctcgc tcatgcttcg accattccct ctttggggcg
    49561 gtaggtcaag cccttgagca tgaggatcgg ccggcgacct atgatcggct cgaccgattg
    49621 acccgcaagc caatgagatg aaggaaccgt gagcgagaag cagtcccgag aggcgtacat
    49681 cctcgacctc ctcgcgaagg acggatcgct atcggtggca gcactctcgc gtgacctggg
    49741 tgtctccgag gtgacgatcc gcaccaacct gcgcgacctg gaggctcgcg gcctgcttct
    49801 gcgcacccac ggcggcgccc aggcgagcag ctttcagacc gtgctggagc ggcagcgtca
    49861 ccacatggcc gagaaggagc ggatcgcggc ggtcgcggcg agcctcgtcg aggacggcga
    49921 cagcctgatg gtcgaagccg gcaccacgac ggcactgctg ctgaagagcc tgctgggacg
    49981 ccaggggctg caggtggtga cgaactccac gctgacgatt ccggcggcgc ggctgaaccc
    50041 cggcctgcgg ctgatcgtca ccggcgggct gttccaccac gagaccgagt cgttcaccgg
    50101 ctccggcgcg ttggcgacga tccgcgacta caacgtgcgg ctcgcgttca tcggcaccga
    50161 cgggttcacc gcagaacgcg gcctgactac gcagttcgcc gaaggggcgg acgcgatccg
    50221 cgccatgcac tcccgcgcgg aggagacctg gctgctcgcg gattcgtcca agtacggccg
    50281 cgcgggcttc gtgaacgtgc taccgctgca cgacgtcgcc ggcgtcatca ccgatgccgg
    50341 tatcggcgag gagtcggtgt cggcgctgcg cgagctcggc gtccgggtgc tgatcgcctg
    50401 agccgcgccg tcaaggtgga cggttcctcg ctgtgacgcc gccaaaccgt ccactctggc
    50461 gatcggacac gcgctgaagc cgccaccgcc aaatggagtg gaccgttgct cggcgacaca
    50521 ccgcgccaaa ccgtccactc tggcgatcgg acacgcgctg aagccgccac cgcccaaatg
    50581 gagtggaccg cttctcggca acaccgcgcc aaaccgtcca ctctggtgga gcccggctgg
    50641 gatgcaaaca ggcagcgcgg gaatcggcat gcgcagcgcg gcgactgggt agaagagagc
    50701 catggacttc actgggaagg ttgcgctggt gaccggcgcg ggctcgggca tcggcgaggc
    50761 ttgtgccgtc cggctggccg aactgggcgc ggaggtggcg gtagtcgggc acagcctcga
    50821 cagtgccgcc gcggtggttg agcagatccg gacgcgaggt ggcgcggctg agccgttcgc
    50881 cgccgaagtg agcgatgcgg acgccgtcga gcgcctcttc gagcaggtcg gacgccgctg
    50941 gaacaaactg cacgtagttg tggccaacgc cggcatcaac ggcgtctggg cgcctctcga
    51001 agagctgagc gtcgaggagt ggtcccgcac catcgaggtg aacctgaccg gcactttcct
    51061 caccgtgaag tatgcgctgc cgctgctcaa ggtcgtggtc acagcttcgg tgaacggcac
    51121 ccgaatgttc tccaacagcg gcgcatccgc gtacgccacc agcaaggccg gtcaggttgc
    51181 gttcgcgaag atggccgcgg tcgaactggc ccgccaccgc gttcgggtga atgtcatctg
    51241 tcccggggca atcgagaccg aaatcgatga caacaccgag cagcggaaca ctgacggcct
    51301 gcacgttccg gtggagtacc cgcatggtca gattccgctc accggttcga ggcccggatc
    51361 ggcagctcag gtcgccgagc tcatctgttt cctgggctcg gacgcagccg accacgtcac
    51421 tggcaccgag gtctggatcg acggcgccca gtcgctgctg gccggctgaa ctggacggtc
    51481 cgcggagggt acgcagagcg cgagcggact gggctcaggg catagaaacg agggttgtga
    51541 gcccgaaaag gccactcaca accctcattt cgatgggcag tactgcagca aattcgtcag
    51601 gctcagccgg cgttgccctc cacggctgaa cgagcttcgc gcttcacgtc gtcggcagcc
    51661 gacttggcgg tgccagcaac gtcggacgca gcttcgccgg cgacctcctt cacctccgcc
    51721 acggcgctgg cgccctgttc ctggacgtgc tggacggcgc cggtagccga ctccttcagc
    51781 gaatccaccg cctgctggac cggctcggac aggttctgcg ccacctcttg gccggcagcc
    51841 gccagctgat cggtcaacgg ctgggcctgc tccttcacgg cttgggcaag ctccttctcc
    51901 ttgcgtgacg gcgggaacgc ggccgcgatc aacagcccga tcccgaaggc caccactccg
    51961 gcggccaacg gattgccctg ggtctgctgg cgggcggccc gcggcactcc ggccacactg
    52021 tcaccgacct ccgacgcccg tccggcgacc ccgctggccg cgtcgtgaac gccttccaca
    52081 gcgtgctgca cgcctccgag cgctgcgtcc ttcacgtcct cagcggtccc aagcagccga
    52141 tcgaggaagc gtccgccggc ggcctttacc ttcgacacct gtcggtgtgc gatgtgtgca
    52201 gggttcgcct ggtcggccag cgcgtccacg ttgtccgaca ggctgctcct ggtgtgttcg
    52261 atctgcgccc ggatctcgga cgggttgctg gtcatcggtt gtcctcctcg ttgcccttca
    52321 atgcgttcgg aatcttgctg acggtctccg ccgtcttcgg taggccgcgt acccgggtca
    52381 gctcggtgcg tccgctggcg gcgaggatcg ccgcgacgat cccccagacc acggctacga
    52441 cgaccgctga ccaggcgttg ccgatcaggt acccgagccc ccaccacagg gcgatcgata
    52501 agaacagcac gctcaggaat gccccgacgc ccgcgccggt gagcaggcca gcgccgcgtc
    52561 cggcctggct ggcggactca cgcaactcgg ccttcgccaa ctcgacctcc tgacgcatca
    52621 aagtcgacag gtcgcggctc acgtccccga gcagttcacc gatcgagcgt tcctcgggca
    52681 cgactgccgg tactgcagct ggctggccca ctgggtccag cggtccgctc atcgcagcac
    52741 accacccgga gcgccgagat ccggctcgac cgcaagctgg ggttcggtca ccctggtcgt
    52801 cgtcggcacg gcccggacgc ctgcgctcga ccccagctcg ctgtagccgc ttgcccgggc
    52861 aacactcggc tggcccggcc ggctctcatc ggcggccatc agcccccggc tcaggcgtcc
    52921 gaccaccatc ccggcgaccg cagcaccggc caggaagacg aacggacgcc gtcgcgcgaa
    52981 ccgtttgatc tcgaccagca catcggcagg ttcggattcc tgcagccagt gcgaaagttc
    53041 accgccgcgc gctgcggcct gcttggcgag ggccgtcaac gggccgccgt cgtccgactt
    53101 cgacgccatc tgacccagct ccgcggccag cgagtgcagg aggtcgccca gttgctgctt
    53161 gccgttgccg gcctgctcgc gcaactgtcc ggacgcctgc gaccagagcc cctgcacctg
    53221 cgtccgcgcg tcgctggcca gcttggccag ctcgtccttg gccactccgg ctacctcggc
    53281 cgcgtccgcc aagacggtgt ccttgaccac gccggcctgc tctgccccgg tttcggccag
    53341 gtgctgtgca gactcggcca cgcccggttg gcggttgtag tcgggtacgc ccacgcttcc
    53401 ggtctggatg ccttcgttca gcttcactcc ttcatccggt tgcccgacga ttcgccgggc
    53461 gcttggtgat tgggggttac ccctgcatcg gcggcctaat cgggcccgct gccacctcgg
    53521 cggccgcgcg ggtcgacgcg tttcggcccg cccagcgcgg ggaacgacct ctgcgcaggc
    53581 acccgcgcgc cgatcggcag gagacagcat ggcgttgacg caaatcgtcc tgatccgcca
    53641 cggcgaaagc ctcgggaacg tggccgcggc ggcggcgaac gcggccgggt tggaagcgat
    53701 cgaggcgggc gcacgcgacc cggacgtcgc gctctccccc gccgggcttg agcaggcaac
    53761 ggcgctccgc cgcgggctgt cgctcctact gtcggacgga gtcagcaccg ccatctggtc
    53821 ctcgccgtac cggcgggccg tgcagacggc agaggtcgca ctggccgctg gtgtcgcacg
    53881 gccggcgatc cggatcgacg agcgcctgcg cgaccgcgag ctgggcgtcc tcgacctgct
    53941 gacg
//
LOCUS       DAOPRQ010000004        47585 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_557_length_47585_cov_11.272039, whole genome shotgun sequence.
ACCESSION   DAOPRQ010000004 DAOPRQ010000000
VERSION     DAOPRQ010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 47585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 47585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47585
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_557_length_47585_cov_11.272039"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..982)
                     /gene="aspS"
                     /locus_tag="PKJ61_00825"
     CDS             complement(<1..982)
                     /gene="aspS"
                     /locus_tag="PKJ61_00825"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009639998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOC12408.1"
                     /translation="MIRTHNAGSLRPEHVGTPVTLAGWVAKRRDLGGVAFLDLRDASG
                     IVQVVVRDDILETSGAHALRNEYCISVTGVVEARSADTVNPALATGEIEVAASGLEVL
                     NPSAPLPFQLDERTTVNEDARLKHRYLDLRRGRMHDALVLRSAVTRAIRETLDGLEFY
                     DIETPTLTRSTPEGARDFVVPARLHPGSWYALPQSPQLFKQLLMVAGMERYYQIARCY
                     RDEDFRADRQPEFTQLDVEMSFVDQDDVIAVAEKVMAACWKLIGVELPLPLPRMTWAD
                     AMADYGSDKPDLRFENKIRELTSFFAETPFRVFQAEYVGGVVMPGGGSQPR"
     gene            complement(1006..2337)
                     /gene="hisS"
                     /locus_tag="PKJ61_00830"
     CDS             complement(1006..2337)
                     /gene="hisS"
                     /locus_tag="PKJ61_00830"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOC12409.1"
                     /translation="MSRPRALSGFPEFTPSGRIVEQRVLASLAQTFELHGFGSIETRA
                     VEPMDQLTRKGEIDKEVFVVRRLHADASARDELGLHFDLTVPFARYVLENSGHLQFPF
                     RRYQIQKAWRGERPQEGRYREFTQADIDIVGSQTLAGHHDVELPLVTLEALEKLHVDF
                     GVPPATMSVNNRQLSEGFYRGLGIEDHLAVLQRVDKLDKIGPDAVRTLLVDEVGLSSA
                     AADACLKLAGVRSADASFVEQVTALGVEHPLLTEGLELLAGVVSAANTLVPGRLVADL
                     KIARGLDYYTGTVYETTMAGYESMGSISSGGRYDSLASDGRTTYPGVGLSIGVSRILV
                     PLVGKGVLTASRPVPSCVLVAVDDEESRFAAMATAAQLRARGIATEVAPKADKFGKQI
                     RYADRRGIPFVWFASGEVKDIRSGEQVPADADSWLPPDADLRPSVVRSPSA"
     gene            complement(2337..3023)
                     /locus_tag="PKJ61_00835"
     CDS             complement(2337..3023)
                     /locus_tag="PKJ61_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOC12410.1"
                     /translation="MFLASFASGLWQANCYVVAPADATTCVVIDPGQNAAEVLEEVLL
                     EHRLAPAGVLLTHGHFDHVASAANFAEAHQVPVWVHPADRHLLSDPAAGLSTDSADLV
                     QQLLGGPLAEPADVRSLAVDSVALAGLEFSVTHAPGHTAGSVLLGLPYPDHPPIGRIV
                     FSGDVVFAGSVGRTDLPGGDQATMLRTLQDIVLALPDDTALLPGHGDQTTMAAERAQN
                     PYLQPAFLRN"
     gene            3099..4343
                     /locus_tag="PKJ61_00840"
     CDS             3099..4343
                     /locus_tag="PKJ61_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013161060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HOC12411.1"
                     /translation="MTSTSGPADFGRVDADGTVYVRTADGERAVGQIPDVEPAEAMAF
                     YTRRYEALEVEVGLLETRVKGGALSPEEARGSINTVRQTVGSANAVGDLDALLARLES
                     LQPLLAEQAEARKAERSRQQEATRETKEKMVAEAEALALGTDWRGGVNRFRTLLDEWK
                     ALPRIDRASDDALWHRFSAARTTYTRRRKSQFAEQAAKRDVAKATKEQIIAEARELAK
                     STDWGPTAGAFRDLMTRWKAAGVAPREVDDQLWAEFRGIQDGFFGARTAVLTEQDAEF
                     KANLDAKLVLLDEAEASLVPVTDLAAARAGFRTFLEKYNAYGRVPREQMRSLDARVKA
                     LETAVRSAEEDEWRRTDPQARARAEETVGMLSAEIEKLTEKVAKASARGDASAAKKAQ
                     DSIDTYTVWLDQAKATLADFSR"
     gene            complement(4490..6697)
                     /locus_tag="PKJ61_00845"
     CDS             complement(4490..6697)
                     /locus_tag="PKJ61_00845"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002544969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HOC12412.1"
                     /translation="MRMRQVLARIGAQRNQPAPVLDPLLRIVNTHRPKADVALIERAY
                     RTAEHYHTGQLRKSGDAYITHPLAVATILAELGMNEATLCAALLHDTVEDTSYTVQQL
                     TADFGPEVAAMVDGVTKLDKIAYGESAKAETIRKMVLAMSKDIRVLVIKLADRLHNMR
                     TIGYLRQDKQMAIARDTLEIFAPLAHRLGMNAIKWELEDLSFATLQPKIYDEIVKLVA
                     ARAPLREDYLRVVTEQVRADLAEAGIKATVYGRPKHYYSIYQKMVVRGRDFADIYDLV
                     GLRILVDTQRDCYAALGTLHARWNPIPGRFKDYIAMPKFNLYQSLHTTVLGPGGKAVE
                     LQIRTHEMHRQAEYGVAAHWKYKEGKADGTELNWVQQISQWQRETEDPGEFLDTLRFE
                     IATDEVFVFTPKGDVMNLPQGATPVDFAYAVHTEVGHRTIGARVNGRLVPLESALTSG
                     DVVEVLTSKAANAGPSRDWLGFVKSPRARGKIRQHFMRERREESIENGKDLLTKQLRR
                     AGLPLQRLLTPEHMSGLVEHFRLADTDALYAALGESSISAQAVVQRLITSEGGAEAVD
                     ELRGEDESVISRPRRTRSSSDEAGVVVKGAPNVWVKLARCCRPVPGDEILGFVTRESG
                     VSVHRKDCTNAESLRSEPERLVEVSWAPTASSSFLVAMHVEGLDRTGILSEVTRIMGE
                     HHISIQSATLNTTKDHIFKFRFTFETTDPTHLEHLIEAVRKVPGVYDVFRLKN"
     gene            complement(6819..7895)
                     /locus_tag="PKJ61_00850"
     CDS             complement(6819..7895)
                     /locus_tag="PKJ61_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020391307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HOC12413.1"
                     /translation="MDGIRPRIGLSRQPWTGRAQVHLPATTSSAARQLLLRVLVGVGL
                     LLALTLIVYIDRDGYSDNVAQDGISFVDALYYATVTMTTTGYGDITPVGVQARIINIF
                     VVTPIRIAFLVLLVGTTVEVLANEGRRALQDSRWRKTMRNHTVIIGYGTTGQSAAATL
                     QRGSVPVERTLVIDTDPQAVAAANRHGLAAFEGDATSRELLHRAELPKAREIIITVGR
                     DDTAILTTLTVRQLNRGAHVVVVVRDSDNVPLIRQSGADAVITSSDAVGRLMGLSSIS
                     PHLGDIIEDLLSSGQGLEVTQRPITAEESGKSITEIQGEKVLGVVRNRTLRRYYEASA
                     DQLRTGDEVIVVRRAPGHRTPRVE"
     gene            complement(7895..8443)
                     /locus_tag="PKJ61_00855"
     CDS             complement(7895..8443)
                     /locus_tag="PKJ61_00855"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002517735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HOC12414.1"
                     /translation="MTAPTAVQNRELIASLIRDIPDFPKPGVLFKDITPLLSSAAGYR
                     AAVTELANTAPAGIDVVVGMEARGFIFAGPVALELGAGFVPVRKPGKLPGDVYTQTFS
                     LEYGHETLAVHRDAIPPGSRVLVIDDVLATGGTIGATAALIEKLGAELIHVSVLLELS
                     FLGGRKLLADAGITETSAVITV"
     gene            complement(8440..9624)
                     /gene="secF"
                     /locus_tag="PKJ61_00860"
     CDS             complement(8440..9624)
                     /gene="secF"
                     /locus_tag="PKJ61_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013161063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HOC12415.1"
                     /translation="MSTEVIETPQKPKKSGLAHKLYTGEVSYDFVAKRKRWYLISAVL
                     LLVSLGALLIKGPTWGIEFTGGADFQAPIKVTANTVDDVRAAVAELNLPNADDISVTT
                     VGDSSVRVQTKALTTEQVTQVKGAIAAVAGTQPDEVGYSLIGASWGQQITTQALIALA
                     VFLALVMLLIWAYFRDFKMSIAAIAALLHDLIVTVGVYALVGFTVTPATMIGVLTILG
                     YSLYDTVVVFDRIRENTRDITNTKHTYSVLANQSLNQVLVRSLNTTIIGVLPVAALLF
                     TGWFVLGTGPLKDLGLALFIGMIAGAYSSIFIATPLLAQMREADSDMKEHRERLARRA
                     ARTSERATHKPTAKPVSTVAVGDPEVAAVDPATLGLVSAKDLASGERRQPTRQPRSQR
                     KK"
     gene            complement(9621..>10081)
                     /locus_tag="PKJ61_00865"
     CDS             complement(9621..>10081)
                     /locus_tag="PKJ61_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016455948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOC12416.1"
                     /translation="GASMGFALNLPGIAGAIVAIGVTADSFIIYFERIRDEVRDGRSL
                     RSAIETGWTLSKKTILVADSVSLLSAVVLFVIAIGAVKGFAFTLGLTTLIDIAVVFFF
                     TKPMMTLLGRTKFFGEGHRFSGFEAEHLGSANPPLHNSRRTAAVAKGAQA"
     assembly_gap    10082..10181
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10182..11458)
                     /locus_tag="PKJ61_00870"
     CDS             complement(<10182..11458)
                     /locus_tag="PKJ61_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HOC12417.1"
                     /translation="MATASRNHTHPLRTLLILALVTAALYGLMALSGSWMPKLGLDLR
                     GGTTITLTAANTSGSGAVDPTSLELARGIIQQRVDSMGVGETEVTTSGESQIVVSVPN
                     VQQDDLVKMVGTTAQLYFRKVYAAEAAGGTAVPDPSASAEPGETATTDPGTEPTASAS
                     ASPTANKRPLPELPTPEPSPRPTAPSGETKELAELLQWTPSDRDNTDFANFKCGDKFP
                     DVTDQPLIACDEDGLYKYLLGPVLINGSDVTRATAGVPQNSVSYEVSLEFNANGGARF
                     YTVTAHLNEQTSPQNQFGIVLDSKVISAPEVTKGAISGGRASISGNFTQASATSLANV
                     LSYGALPLSFEVSSVDNVSAKLGGDQLQAGIIAGLIGLGLVLLYSILYYRGLALVVSL
                     SLVVAGLLSWAMIVLLGASMGFALNLPGIAGAIV"
     gene            complement(11477..11929)
                     /gene="yajC"
                     /locus_tag="PKJ61_00875"
     CDS             complement(11477..11929)
                     /gene="yajC"
                     /locus_tag="PKJ61_00875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HOC12418.1"
                     /translation="MDTVLLIVIMGAALYFLMIRPQQKRAKEQKNLMEAIAVGSRIMT
                     VSGIIGTIKHLGEKQAVLEISPGVELTIDKRAISPQAVEDEFEYSDDADVEAADADGV
                     VAEPFQIEDGAPVEDATPVEDVPVVGADPEDVTEPAPITWENPDTQKN"
     gene            complement(12041..13093)
                     /gene="ruvB"
                     /locus_tag="PKJ61_00880"
     CDS             complement(12041..13093)
                     /gene="ruvB"
                     /locus_tag="PKJ61_00880"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOC12419.1"
                     /translation="MTTDESDPLDPHAFVDEQAAESALRPGSLAEFRGQQRVSDQLGL
                     VLAGARHRDAVPDHVLLSGPPGLGKTTLAMIIAAEMGAPIRITSGPAIQHAGDLAAIL
                     SGLTEGEVFFLDEIHRMSRPAEEMLYLAMEDFRVDVVVGKGPGATAIPLDIPRFTLVG
                     ATTRAGLLPGPLRDRFGFTAQLDFYAASDLETILTRSAALLGIHLDGQAAKEIASRSR
                     GTPRIANRLLRRVRDYAQVRADGTLSRSVARKALELYEVDPLGLDRLDRAVLDALCRH
                     FGGGPVGLTTLALAVAEETETVEEVAEPFLVREGFLMRTPRGRVATARAWQHLGLTPP
                     GHNQPPIAAPDLFSDG"
     gene            complement(13099..13701)
                     /gene="ruvA"
                     /locus_tag="PKJ61_00885"
     CDS             complement(13099..13701)
                     /gene="ruvA"
                     /locus_tag="PKJ61_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002526836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HOC12420.1"
                     /translation="MIAQLTGVVAAVGGTWLVLDLSGFGLKALCTPATATSVRVGELV
                     TLHTSLVVREDSLTLYAFAEPGERDCFELVQSATGIGPKIAQAVVSVFSPDEFRTAVA
                     SGNLAALTRVPGIGAKGAQRLVLELKDKVAALGTGATVVGATIAGSAWREQVRAGLEG
                     LGYSTRDAEAACDRVSTLAEETPDTPVAVLLRAALRTLAK"
     gene            complement(13698..14225)
                     /gene="ruvC"
                     /locus_tag="PKJ61_00890"
     CDS             complement(13698..14225)
                     /gene="ruvC"
                     /locus_tag="PKJ61_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008356869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOC12421.1"
                     /translation="MRVLGVDPGLTRCGVGVIDGSPGRPPQLVAVGVVNTPSTLELPQ
                     RLLRIEAGLTEWVNQHRPDAIAVERVFADQNVLSVIGTAQAAGLAMLVAARAGIPVHT
                     HTPTEVKAAITGSGRAEKAQVGAMVARILKLEAPPKPADAADAVALAICHLWRGAGAD
                     RIAAAIAKQTAGRTA"
     gene            complement(14367..15131)
                     /locus_tag="PKJ61_00895"
     CDS             complement(14367..15131)
                     /locus_tag="PKJ61_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008356873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOC12422.1"
                     /translation="MSGHSKWATTKHKKAAIDAKRGKLFAKLIKNIEVAARTGGADPS
                     GNPTLYDAIQKARKNSVPVDNIDRAVKRGGGVGGDAVDYTEIMYEAYGPGGVGLLIEC
                     LTDNRNRSASDVRVAVTRNGGTMADVGSVSRIFDRKGVVEVAKSQTGRTLTEDDLLEA
                     TLDADPEDVSDEGETFKVISDPSLVVDVRKAVQAAGLDYESAEVQFVPQYTTAVDTAE
                     IAEKLFKLLDAIEDLDDVQNVYSNEDVPDEIGEQLA"
     gene            15434..16522
                     /locus_tag="PKJ61_00900"
     CDS             15434..16522
                     /locus_tag="PKJ61_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013160955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoate A/B protein ligase family
                     protein"
                     /protein_id="HOC12423.1"
                     /translation="MSERLHGEYKVPGGKLVAVDLSVVDERLAQVSVSGDFFLDPDEA
                     LSVIDRSLAGLSVELGVGELAARIDSDLARASADGLLGVPVTMVGFDSRAIAIAVRRA
                     LGRSTGWSDHIFTFLNPGPLHPAEHCALDEVLARELAAGRRGPTFRFWEWDSAAVVIG
                     SFQSLRNEVDAEAAATHGVEVVRRVSGGGAMFMEAGNCITFSLVVPATLVDGMSYEAS
                     YAFLDEWVLAALDEVGVQAHYAGLNDIASPAGKIAGAAQKRFVGGAVLHHVTMAYDID
                     ADKMLQVLRIGREKLSDKGTKSANKRVDPVRSQTHLPRAEVMASFEATFRNRYRVVEG
                     QLSPAELAAVKELTQTKFTNVDWIGRVP"
     gene            complement(16532..16990)
                     /locus_tag="PKJ61_00905"
     CDS             complement(16532..16990)
                     /locus_tag="PKJ61_00905"
                     /inference="COORDINATES: protein motif:HMM:NF013995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HOC12424.1"
                     /translation="MKTSSSLNPTRYWVNTVSLDQVRRDVAGGYLQANPSVRSRLARL
                     NPGDQVVCYSPRQSDPDGRPLQQFTACGTVVGAEPYEARVADGSRPWRIGVRFAECGG
                     AGVRPLLGTLGFVSDPAHWGMVFRRGMFEIPRDDFVRIADAMQAAELLAG"
     gene            complement(17209..17928)
                     /gene="priA"
                     /locus_tag="PKJ61_00910"
     CDS             complement(17209..17928)
                     /gene="priA"
                     /locus_tag="PKJ61_00910"
                     /EC_number="5.3.1.16"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     1-(5-phosphoribosyl)-5-((5-
                     phosphoribosylamino)methylideneamino)imidazole-4-
                     carboxamide isomerase/phosphoribosylanthranilate isomerase
                     PriA"
                     /protein_id="HOC12425.1"
                     /translation="MRLELLPAVDVQGGQAVQLTQGVAGSEKVFGDPLAAALRWQDGG
                     AEWLHLVDLDAAFGRGSNAEVIAEIVAKMALKVELSGGIRDDASLQRALATGCRRVNI
                     GTAALEHPEWCDRIIADHGDRIAIGLDVRGTRLAARGWTREGGELWETLDRLNAAGCA
                     RYVVTDVASDGMLTGPNLQLLTDVCARTDAKVVASGGISTLDDLRALRELVPQGVEGA
                     IIGTALYVGNFTLADALAVAG"
     gene            complement(17925..18566)
                     /gene="hisH"
                     /locus_tag="PKJ61_00915"
     CDS             complement(17925..18566)
                     /gene="hisH"
                     /locus_tag="PKJ61_00915"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017933845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HOC12426.1"
                     /translation="MTSRDPLVAVLDYGSGNLHSVVRALVAAGAEVVLTSDPDACLAA
                     EGLVVPGVGAFAACMDGLASVGGPQLILRRLAAKRPVLGICVGHQVLFALGVEHGEPT
                     PGVGVLPGVVAELAAVPLPHMGWNRVEPPTASAMFAGVEHERFYFVHSYGVHEVPDWP
                     GTARISWAEHGGDRFIAAVEQGALWSTQFHPEKSGAAGARLLRNWLGTLRKES"
     gene            complement(18563..19177)
                     /gene="hisB"
                     /locus_tag="PKJ61_00920"
     CDS             complement(18563..19177)
                     /gene="hisB"
                     /locus_tag="PKJ61_00920"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008362999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HOC12427.1"
                     /translation="MSPRTAVRTRSTSESEVRVALNLDGTGQSTISTGVGFYDHMLTA
                     LAKHSLIDLEITTTGDVHIDGHHSVEDTAIVLGQAFAEALGDKAGVRRYADATVPLDE
                     ALAHCVVDLAGRPYVVYSGEPEGQQYALIGGSGVPYAGSMTQHVIESFAINAGITLHL
                     RVLAGRDPHHIVEAQFKALARALRDAVAIDPRIAGQVPSTKGSL"
     gene            complement(19174..20259)
                     /locus_tag="PKJ61_00925"
     CDS             complement(19174..20259)
                     /locus_tag="PKJ61_00925"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016669831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOC12428.1"
                     /translation="MRPDANLGELPLRPELVGEEPYGAPQLDVPVRLNVNENPYPPGP
                     EIRAEMASAVAAESLNRYPDREAWALREALADYLGHGLIPLQIWAANGSNEVMTHLLQ
                     GFGGPGRKVLSFTPTYSMYPEYARNTHTTYVTEPRLPDYSLDADTVLKAIAEHEPAVV
                     VITTPNNPTGTTTPLEVVEAVLTGTEAIVVVDEAYQEFARHPEQTALTLLPRFGNLVV
                     SRTMSKAFALAGGRVGYLAASPAIVDACRIVRLPYHLSAQTQAVARVALAHSAELLAQ
                     VDELRVTRDQMLVRLPQLGVEVIDSDANFCLFGPFADRHDVWQRLLERGVLVRETGPA
                     GYLRVSAGTPAETQAFYAALAEVMEER"
     gene            complement(20256..21524)
                     /gene="hisD"
                     /locus_tag="PKJ61_00930"
     CDS             complement(20256..21524)
                     /gene="hisD"
                     /locus_tag="PKJ61_00930"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008363003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HOC12429.1"
                     /translation="MPDYRSVLPRAEFDVDAAVEQVRPIVAAVAERGEAALAEFSERF
                     DRVTPRSFRVPAAELAGALSGLDEQVRAAFTEAIRRRRLVAEAEAVSVPARVELAPGA
                     VVEQKLVPVGRVGLYVPGGLAPLASSVLMNVIPAQVAGVSSIAVASPPQAEFGGLPHP
                     NILAVCALLGVEEVYAVGGAQAIAMFAYGVPGLCASVDLVTGPGNIYVVAAKRLLRGL
                     IGIDSEAGPTEIAILADGTAVPRFVAADLISQAEHDPMAASVLVTDSVDLAEAVQAEL
                     EPQVAATKHADRVRTALTGRQSAIVLVDDVAQGLAVVDAYAAEHLEIHTADAAAVAAR
                     VRNAGAIFVGPYSPVSLGDYSAGSTHVLPTAGCACHSSGLNVHSFRKQVHVIDYSEAA
                     LLAVAGVVETFAAAEDLPAHGAAVTVRRPA"
     gene            21657..22394
                     /locus_tag="PKJ61_00935"
     CDS             21657..22394
                     /locus_tag="PKJ61_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007077554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HOC12430.1"
                     /translation="MFIGFGTAVNVVTVVIGSALGVLVGHRLPHRTRELVTQVLGLVT
                     LVIGGLSIASGMSEAFAAEVGAQARLLIVLGSLLIGGIIGSLLQIENRLDRGAQLLHH
                     RFASGSEGSTFVEGAVTATLVFCVGPLSILGALSDGLGQGAEQLLVKAVMDGFAAIAF
                     AASLGIGVMASVIPLAIYQGLLTLLGFALGNFLPVGQVDALGSVGGVILLGLGFRLAG
                     IKQIPVGDLLPALVMAPLLTAGVGMLV"
     gene            22394..22876
                     /gene="ybaK"
                     /locus_tag="PKJ61_00940"
     CDS             22394..22876
                     /gene="ybaK"
                     /locus_tag="PKJ61_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008363006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HOC12431.1"
                     /translation="MAGATPAIEALLAAGIAHTLHPYDHDPLVTAYGDETVAALGVDP
                     LRVFKTLVLDVGATRPELAVGVVPVAGQLDLKHFATALGVKKAAMAAADLVARSTGYV
                     LGGVSPLAQKNSLPTIIDETAELWDTIYVSAGRRGLQVELAPNDLAAVVSARFADIAR
                     "
     gene            complement(22894..24354)
                     /locus_tag="PKJ61_00945"
     CDS             complement(22894..24354)
                     /locus_tag="PKJ61_00945"
                     /inference="COORDINATES: protein motif:HMM:NF017085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH and CHAD domain-containing protein"
                     /protein_id="HOC12432.1"
                     /translation="MAQLIEAERKYALTTGQELPDLAAVITPGPVGEFELTATYYDAP
                     DLRLTRAWQVIRHRVGGADAGWHLKLPGPTSDQRIEVHADGAAPRVPDEFRRRVATTL
                     AGAPLVPVAVLRTNRREQQLLGAGGDVLAVSCTDDVVASVGARRERWREAEIELVGGD
                     LGLLDRLEAVLTAHGIDRAQSGSKIGRALADAIAAQRALDGRSSAGEAVHAYLAEQVG
                     VLQNREADVLVDAPDAVHRSRVATRRLRSALRTYARVFADQTGTLRNELRWHAEELGA
                     PRDAEVLQSRLAEAVRQLPETDRLLVGERLQRSLGSTHAAAHAKLVASMRTAGYEALQ
                     LALSRLLAEPQWSPAAQRPAAELLPRLLARAVARVGKLAEHAASRPSDLTRWHEVRKA
                     AKAARYGSELLVPVLGAGAEVWRARWEAVTEGLGAVQDAVVAQQVIGDVAWHAVADGL
                     PRLPFDDLRQHEDRLLRESLAAGRAALADALALDVP"
     gene            complement(24357..24998)
                     /locus_tag="PKJ61_00950"
     CDS             complement(24357..24998)
                     /locus_tag="PKJ61_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC12433.1"
                     /translation="MSTTVSEGSPEAIAPEPVRGVASWIGLLAVLCLVVGALAGVLWS
                     LGTDLPSYVILQQDGSASISERGLTEVVAADVWFVVTGALVGAGLGLVVWRWFSALGW
                     PTALLAVAAGLLAGVVCWQIGELIGPNNFADRLGAAQAGDLVPVSLELRSISALAIWG
                     LAAVTPVLLASSFGPDDEEGTDNWWRRASDDEPEDAGTVDERGVLTATDEATP"
     gene            complement(25009..28527)
                     /gene="dnaE"
                     /locus_tag="PKJ61_00955"
     CDS             complement(25009..28527)
                     /gene="dnaE"
                     /locus_tag="PKJ61_00955"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016455935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOC12434.1"
                     /translation="MAEEFVHLHCHSEYSMLDGAARLKQLVSRAEQLDMSALAVTDHG
                     NMFGAYEFYKAAKGSSVKPIIGLEAYLTPKTHRSERKRVQFGDGTGDDVAAKGAYTHM
                     TIWAQHNEGMHNLFRLSSLSSLEGFFYKPRADRELLNRYAKGLIGTTGCPSGEVQTYL
                     RLGQYAQARESAAEFRDIFGAQNYFVELMDHGLDIETRVRADLLRLAKDLQLPLVATN
                     DLHYVHASDASDHDTLLCVSAGANKDTPNRFKFDGDGYYLKSPAEMRALFSNLPEACD
                     NTLAIAERCETSFDEGSGTYMPVFPVPAGETESSWFEREVAAGLERRFRGDVPEYATK
                     QAAYEQDVIIGKGYAGYFLVVADFINWAKRNGIRVGPGRGSGAGSMCAYAMGITDLDP
                     VPHGLIFERFLNPERPSMPDFDVDFDDRRRGEVIRYVTEKYGDDRVCQIVTYGTIKAK
                     QAIKDAGRVLGMPFSLGEKLTKAYTPPVQGKDVPLADVFNPEHDRYGEGTEFRELYES
                     DPDAKLVVDTARGIEGLKRQWGVHAAGVIMSSAPLLDVIPIMKREQDGQVITQFDYPS
                     CEDLGLVKMDFLGLRNLTILDDAVRNVKANRGVDIDLDALRRDMSDPAAYELLASGET
                     LGVFQLDGGGLRSLLKMMRPDNFEDISATIALYRPGPMGANSHTNYALRKTGRQPVEP
                     IHPELAEPLADILDTTYGLIVYQEQIQQIAQRVAGYSLGQADLLRRAMGKKKQSILDA
                     EFEPFSTGMRERGYSNASIKTLWDIMVPFAAYAFNKAHSAAYGVISYWTAYLKANFPA
                     EYMAALLESVKSDKDKTALYLGECRRMGINVLPPDVNASDGMFTPVEQDIRYGLAAIR
                     NVGDNVVRGIVEARTERGKASDFNAFLDQVPLVVCNKRVIESLIKAGAFDSMGHTRRG
                     LMECFERRVDEVIDLKRNQANGQDDLFGDFGGTEQIAASPVPPLPEWDKRVRLGFERE
                     MLGLYVSDHPLQGLEHILAAHREYSIGQLRADDGPRDGVVTIAGMITQVVRKQTKNGD
                     LWAIATVEDLESSMEVLLFPKVYAGVAASLATDTVVRIKGRIRTKDDSLEMQGNEVAF
                     PDIAEGPSGPLLIQLPAVRCTPAVVEQLRQVLRAHPGPTEVRVKLVSATAGSKIWRLD
                     DGLRVAPSRPLMADLKALLGPSCVNA"
     gene            28751..29128
                     /locus_tag="PKJ61_00960"
     CDS             28751..29128
                     /locus_tag="PKJ61_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC12435.1"
                     /translation="MNTSHEQRDRIARQAFKNLGQSPSRKAELTVQCDSSHHLAGVYA
                     TDVGLVFHSVLHARARGRRDYADVGHHASRLGLDWFDLLDPPAHAGVPDELSAGCECG
                     PYPLSRALLPQQIAAGAKTVIVP"
     gene            29219..30148
                     /gene="rocF"
                     /locus_tag="PKJ61_00965"
     CDS             29219..30148
                     /gene="rocF"
                     /locus_tag="PKJ61_00965"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014847083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004053 - arginase activity [Evidence
                     IEA];
                     GO_process: GO:0019547 - arginine catabolic process to
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HOC12436.1"
                     /translation="MQQVSLIGAPTDIGASRKGCRLGPQALRVAGIGTALRKFNCEVK
                     DTGDLTGPPNPESPPVNGYRHLAEVIDWNRSVYAATAAELADGRLPILLGGDHCLAIG
                     SISAVAAHCRATGKKLRIIWVDAHADFNTAELTPSGNIHGMPVACLAGHGPDELTTLG
                     GSAPAIRTAEIRQVGLRSVDPGEKALLFAEGVEVFDMRFVDEIGMRRTMTRALAGVDE
                     DTHLHLSLDLDFVDPEIAPGVGTPVVGGPTYREAQLVMEMIADTGRLASMDLVELNPA
                     LDVRNATAELAVDLVESLFGKSTLHRLHEGIAL"
     gene            complement(30235..30810)
                     /locus_tag="PKJ61_00970"
     CDS             complement(30235..30810)
                     /locus_tag="PKJ61_00970"
                     /inference="COORDINATES: protein motif:HMM:TIGR02246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HOC12437.1"
                     /translation="MSPKSSVSFKKLRQLATSAVRLPLPAGGSPVREPLEVVRRFTKA
                     WNRHDADGIAELFTDDVDFVNVVGLWWTSPRSIRRAHKRGFERMFGATELVVEKLKLR
                     WLGEDAALVHARWRMTGQVDPEGQPAQARRGIASVVVQRLADDSWIAVSWQNTDIAPS
                     ADTNLNVAGKLAPSSYLERPPADPEPAEDPS"
     gene            complement(30983..32449)
                     /locus_tag="PKJ61_00975"
     CDS             complement(30983..32449)
                     /locus_tag="PKJ61_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016667617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HOC12438.1"
                     /translation="MADPRGFMTTPRKVADRRPVAERVQDWNEVYPGTPGKAVLPIIG
                     KQAGRCMDCGIPFCHQGCPLGNLIPEWNDMIWRDDWRGAAERLHATNNFPEFTGRLCP
                     APCETACVVGINREPVTIKNVEVAIIDRAWDDRRVEPEAPAWHTGRTVAVVGSGPAGL
                     AVAQQLTRAGHTVVVYERADAIGGLLRYGIPEFKMEKKVLDQRLKQMRLEGTVFKTGV
                     DIGTDITGEQLIERFDAVVLAVGSTVARDLPIPGRELAGIHQAMEFLPQANRVAAGQQ
                     LDDQILATGKDVVIIGGGDTGADCLGTSLRQGARSVTQLEILPTPSTERPVNQPWPTY
                     PMIYRVSSAHEEGGERVYSVSTTQFNGDESGNVTGLEVNEVRLENGRFESVPGSNQEL
                     SAQLVLLAMGFTGPQRTGVVEQLGLELDPRGNIVRNADFATTADRVFVCGDAGRGQSL
                     IVWAIAEGRACANGVDAFLQGSSRLPKPILPTDRPLLV"
     gene            complement(32442..36974)
                     /gene="gltB"
                     /locus_tag="PKJ61_00980"
     CDS             complement(32442..36974)
                     /gene="gltB"
                     /locus_tag="PKJ61_00980"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HOC12439.1"
                     /translation="MAKLAMPPHPAEGLYDPAYEHDACGVAFVAQLSGAPSHDIVSQG
                     LTALENLDHRGATGADAAAGDGAGMLLQVPDRFFRQVVDFDLPEPGQYAVGMAFLPQE
                     PTKRASAKRNFEFLATEEGVVVQGWRQVPTDDSTLSPVSAHNMPHFEQVFVSTESLLA
                     GIDLDRAVYPLRQRARNDAGVYFASLSSRTTVYKGMLTTQQLAEVFPDLLDERVESAL
                     ALVHSRFSTNTFPAWELAHPYRLMAHNGEINTVKGNRNWMRAREALLASEVIPGDIER
                     IFPICTPGGSDSASFDEVLELLHLGGRSLPHAVLMMIPEAWENHTEMDAARRAFYAFH
                     SSLMEPWDGPASVTFTDGSLIGAVLDRNGLRPGRYWVTDDGLVVFASEAGVLDIDPAK
                     VVRKGRMKPGRMLLVDLEQHRLINDEEIKTQLAAEHPYADWLEAGRVDLDDLPEQQHV
                     VHSHASVTRRQQVFGFTHEEQRMILAPMANTGVEPIGSMGSDTPVAVLSDHPRTLFDY
                     FAQLFAQVTNPPLDAIREELVTSLAATFGPEANLLEPSEASCRQIVIGYPILDSDQLA
                     KLVRINRDGSMPAFDTHVVRGLYDVEGGGAALKARLDELCEQVSEAIARGCRTLILSD
                     RHSNAELAPIPSLLLTAAIHHHLVREKTRTQVGLIVEAGDVREVHHVALLIGYGAAAV
                     NPYLAFESAEDLARREWLVHVEPEQAVYNVRKSLGKGVLKVMSKMGVSTIASYTGAQI
                     FEALGLSSELVDKYFTGTTSRVEGIGLDEIAEEVAKRHARAYPPDGIPLPHRTLSGGG
                     EYKWRREGPPHLFDPETVFRLQHSTRERRYDIFKQYTARVDDQSERLMTLRGLLEFNS
                     DRSAIDIDEVEPVSEIVKRFSTGAMSYGSISREAHETLAIAMNRLGAKSNTGEGGEDA
                     DRLYDPERRSAIKQVASGRFGVTSDYLSNATDIQIKMAQGAKPGEGGQLPGQKVYPWV
                     AKTRHSTPGVGLISPPPHHDIYSIEDLKQLIHDLKCANPEARVHVKLVAEVGVGTVAA
                     GVSKAKADVVLISGHDGGTGAAPLTSLKHAGGPWELGLAETQQTLLLNGLRDRIVVQC
                     DGQLKTGRDVVIAALLGAEEFGFATAPLVVSGCVMMRVCHLDTCPVGVATQNPELRAM
                     YSGHADFVVNFFEFIAQEVREHLAALGFRTIEEAVGHVEVLNARRAVDHWKARGLDLS
                     PVLHQVELPEGAPLHCVTTQDHGLDDKMDTQLLAMAQPALTSGELVRADLTVRNVDRT
                     LGTILGHHVTKATQGVGLPENTIDFTLRGTAGQSFGAFLPRGITLRLVGDTNDYFGKG
                     LSGGRLTVRPDERAPFAAEEQIIAGNVIAYGATSGELFIRGQVGERFCVRNSGATAVV
                     EGVGDHACEYMTGGEALILGATGRNIAAGMSGGVAWVLDLVPARLNTELVDALPVNAA
                     ELARIRELLAKHRDETESSVADRLLQLSDEELGGRFTTLMPRDYARVLKARAEAELAG
                     LDEETTTKLMMEASHG"
     gene            complement(37201..38139)
                     /gene="lgt"
                     /locus_tag="PKJ61_00985"
     CDS             complement(37201..38139)
                     /gene="lgt"
                     /locus_tag="PKJ61_00985"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016455934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOC12440.1"
                     /translation="MTLFIPSPSISNFQIGPLTIHIYALCLIAGIFAGWTLGVRRWRA
                     RGGTSESFETVLLWTIPLAIVGARIYHVLTHLGDYFGAGATQHWWAIWEGGIAIYGAV
                     GAGALVAWLTSRRQKVSFPALADAIAPGIALGQALGRFGNWFNQELYGQPTTLPWGLE
                     IDPAHRAPGFEQYETFHPTFLYESLWNLFVMGTLLWADKRFRLGRGKVFALYVALYGF
                     GRIFTEHIRLDYSYDTFGSLRFNEAVAILICLIGVAALIWLVRFRPGREEVVEFGAAD
                     GSRPDRPADEQVEEPAEEPGADPDGSPSTSSPDTKD"
     gene            complement(38161..38772)
                     /locus_tag="PKJ61_00990"
     CDS             complement(38161..38772)
                     /locus_tag="PKJ61_00990"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HOC12441.1"
                     /translation="MAGAGLALALLAGCTAPATPSVPVASESASRFQGVELTTPYALP
                     DLTLTDHTGGDFNVRTSSERPVLVFYYGYTNCPDICLGVLTDIASAMNRLSDADRARV
                     QVLFVTVDPARDTPAVLAKYLSRIDPDFIGLTGEQADLDQLMASMGVGVAGIEKAATG
                     YEVNHTAQIVGFDRERRGVLVWPQGTAIGVLKADLETLIRQQG"
     gene            complement(38781..39677)
                     /gene="trpA"
                     /locus_tag="PKJ61_00995"
     CDS             complement(38781..39677)
                     /gene="trpA"
                     /locus_tag="PKJ61_00995"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002513581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HOC12442.1"
                     /translation="MSESTVVEPVETPANLDENGDGSVSVQSSAAAFTAARADGRGAL
                     VGYLPVGYPSVPGSMAALATLCGADGETPGVDLVEIGLPYSDPMMDGPVIQRAGTRAL
                     ERGVRTKDVFTAVETVRSAGRPAVVMTYWNLVDRYGVDAFARDLANAGGSGLITPDLI
                     PDEADDWLAASDRYGLDRVFLVSPSSSDDRIVSTVAAGRGWLYATSVMGVTGTRSASS
                     SAAPDLVARVRSLAPETLVGVGLGVSNGDQAAEVCGFADGVIVGSAFIKPLLAADEAG
                     NPDDLSGLRTVLADLTAGVRRA"
     gene            complement(39674..40930)
                     /gene="trpB"
                     /locus_tag="PKJ61_01000"
     CDS             complement(39674..40930)
                     /gene="trpB"
                     /locus_tag="PKJ61_01000"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002543398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HOC12443.1"
                     /translation="MNSWVLNPTLPDAGGHYDIFGGRYVPEALVAALNQLEAEFDKAM
                     GDAGFWRELDNLRRNYSGRPTPLTEVPRFSRHCGGARVLLKREDLNHTGSHKINNVLG
                     QALLTRRMGKTRVIAETGAGQHGVATATAAALLGMECRVYMGRVDTDRQALNVARMQL
                     LGAEVIAVESGSQTLKDAMNEAMRDWVSSVDNTHYLIGTVAGPHPFPKMVKEFQRVIS
                     AEIREQMLGRYGRLPDAVAACVGGGSNAMGTFAEFIDTPEVALYGFEAGGDGVETGRH
                     AAAILGGSVGVLHGMRTHILQDADGQTLESHSISAGLDYPGVGPEHAWLAATGRANYE
                     AVTDAEALTALQLLTRTEGIMPAIESAHALAGAMRLGQRLAQTDPETEPIIVVCLSGR
                     GDKDVDTAIKYFGLTGKPDAATGGVA"
     gene            complement(40950..41729)
                     /gene="trpC"
                     /locus_tag="PKJ61_01005"
     CDS             complement(40950..41729)
                     /gene="trpC"
                     /locus_tag="PKJ61_01005"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016669810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HOC12444.1"
                     /translation="MTVLDGIIAGVVEDLEQRQAARSLDDVIAACVDQPPALEVLSAL
                     RGPRLGVIAEVKRSSPSKGALAEIQDPAALACEYAAGGANAISVLTEQRRFGGSLADL
                     AAVRAAVATPLLRKDFVVTEYQLWEGRAAGADLALLIVAALSQTQLERFLELGRQLGT
                     TCLVETHTPDEVRRALAAGASLIGVNNRNLKTLEVDLVHFEEMAGLIGDDAVKVAESG
                     ILSTADAARMRAAGADAVLVGEMLVRHGDPRGAVAALRSLA"
     gene            complement(41731..41961)
                     /locus_tag="PKJ61_01010"
     CDS             complement(41731..41961)
                     /locus_tag="PKJ61_01010"
                     /inference="COORDINATES: protein motif:HMM:NF041681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HGxxPAAW family protein"
                     /protein_id="HOC12445.1"
                     /translation="MIESAERATAKVAKEYHHGRTPAAWAGSIIATVAFILGAVAFLT
                     GPNGTLLWAAGVLLVLAIVTGAVLRRMGFGQS"
     gene            complement(42136..43653)
                     /locus_tag="PKJ61_01015"
     CDS             complement(42136..43653)
                     /locus_tag="PKJ61_01015"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015070536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HOC12446.1"
                     /translation="MSVTVTPDLAGFLAAGATQRVVSVHTRLLADDFTPVGLYHQLCG
                     ERPNTFLLESAEGGVWSRYSFVGVNAAATLTERDGLAEWTWADRPITGLPTGGDPLQV
                     LADTLELLHTPRTEGLPPLTSGMVGYLGYDVVRRLEKLPDGNPDDLGIAELAMMLVAD
                     MAVLDHHTGEVWLVANAINFDDSAAHIEDAYADAVRRVQAMAAALQQPSRPLVAVRDE
                     SPVTPQVRRQRTSAEYQAAVSAAIEEIRAGEAFQIVPSQRFETDCPADALDVYRVLRR
                     RNPSPYLYLLRLGGFAIVGSSPEALVKVNAGRATTHPIAGTRPRGATTDADAALEAEL
                     LADPKEAAEHLMLVDLGRNDLGRVCVPGTVAVVEFMKVRRYSHVMHLEAAVSGTLAAG
                     RTALDATLSCFPAGTLSGAPKVRAMEIIDSLEVSRRGLYGGVVGYFDFAGDSDTAIAI
                     RTAVLRDGVAYVQAGAGVVADSVPANEDAESVNKAAAVLGAVAEASRLRRIADES"
     gene            complement(43718..44068)
                     /gene="hisI"
                     /locus_tag="PKJ61_01020"
     CDS             complement(43718..44068)
                     /gene="hisI"
                     /locus_tag="PKJ61_01020"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016667607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HOC12447.1"
                     /translation="MTELNPDVAALLKRNPDGLVPAIVQDATDGRVLMLAWMDDEALR
                     RTLTTKRGTYWSRSRQSYWVKGETSGNTQAVREVRLDCDGDTLLVRVDQSGPACHTGS
                     VTCFDDRVLLAEGA"
     gene            44169..45596
                     /locus_tag="PKJ61_01025"
     CDS             44169..45596
                     /locus_tag="PKJ61_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC12448.1"
                     /translation="MPLREASVRTAVGFGSTSCGRVRNSPHSGHHPQPEGRPPSEKPT
                     MRSFISTSMAQRFQLRPAFGRQPIRMPSRILTLAAALVLGVSYFVVDTTAHAAPATTD
                     IDSTQLVARDATKTFASGVVRNAKGAAAKSGTVTAYAFPGAAVTKNLKVGDSFTLTEV
                     GRAEIQFDGSYALSLDYDAVVQKSGTGGRPVNIDIVASTTDGTAVYSDTALVDPGKKK
                     LTVNAAATPTTKAAASTAGVATRSVAGTSQGLALDLAVTSQSPAKLQEPASIAVAPMA
                     SIPSNCKMVKDLGNVWTVVGQTAATTTNAQQRIYYQEDATTTFGVALKVNGVYTMSGT
                     TTFQASTTVNFPIYTAIGARKHRTQFKYAELSCTYGSTQIAQISARPIAFATGTDELW
                     TTTPSTPASNCAPYLAGSGLNKKNTTASTFSNGVSIKSKIGIDLSSRSGYSATVRHWV
                     NFTKPGKLCGVDGVPGDAPGLMVVK"
     gene            45593..46093
                     /locus_tag="PKJ61_01030"
     CDS             45593..46093
                     /locus_tag="PKJ61_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC12449.1"
                     /translation="MRRLAVAGLAGAATVAVVSWAGCSVQPQLISATEGTTLCVPVNG
                     DGEAYLGHEVFSNRTGSAVSAAAFVPDQVAGASVVGGYFVPVGEPQPGVGNDPALIPH
                     GFTFEPVPPGERVILVLGVKLDDPNLAGQFTGGTLTSTSGGELGRSSTGYHMVPAGEV
                     CDMGEE"
     gene            46155..46787
                     /locus_tag="PKJ61_01035"
     CDS             46155..46787
                     /locus_tag="PKJ61_01035"
                     /inference="COORDINATES: protein motif:HMM:TIGR03085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03085 family metal-binding protein"
                     /protein_id="HOC12450.1"
                     /translation="MSLSPQRAALCDALDTVDPSAPTLCEGWTAHHLAAHVWLRENEL
                     LAPAGAVFKPLAGRLETRMAEVMTQRSYADLVATIRRGPQGFSLFRLPGAEKAANEIE
                     FFVHCEDVRRGDGDRTPRPLDPEFEDYAWGRVRTMAKLMLRRVPVATWLKRHVADEPL
                     RVGKGADIVTLVGPASELLLYLFGRRPAANVTLVGQATTIAVLQRAKLGV"
     gene            46868..>47585
                     /gene="serC"
                     /locus_tag="PKJ61_01040"
     CDS             46868..>47585
                     /gene="serC"
                     /locus_tag="PKJ61_01040"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016668607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HOC12451.1"
                     /translation="MRVYNFSAGPAMLPLPVLEAAQSELTDWQGSGMSVMEVSHRGKD
                     FVACAAQTEALLREVMGVPDNYQVLFLQGGASGQFDAIPLNLTAAGDTVDFLHTGQWS
                     AKAIKAARKAGLNVEVLADEAASKYTTTPAAGSYTVNPAAKYLHYTPNETIGGVEFGY
                     LPEAGDVPLVADLSSTILSRPIDVTSYGLIYAGAQKNMGPSGLCVLLVRDDLVGHARP
                     EVPSIWDYQAMAESDSMLNTP"
BASE COUNT         7577 a        16230 c        16093 g         7585 t
ORIGIN      
        1 gccgcggctg cgaaccgccg ccgggcatca cgacaccgcc gacgtactcg gcctgaaaca
       61 cccggaacgg ggtctcggcg aagaacgacg tcagctcgcg aatcttgttc tcaaaacgca
      121 ggtcgggctt gtcggagccg tagtcggcca tcgcgtccgc ccaggtcatc cgcggcagcg
      181 gcagcggcag ttcgaccccg atcagcttcc agcacgcagc catcaccttt tcggccaccg
      241 cgatcacgtc gtcctggtcg acgaagctca tctcgacgtc cagctgggtg aactcgggct
      301 gccggtcggc ccggaagtcc tcgtcccggt agcagcgggc gatctggtag tagcgctcca
      361 tgccggccac catcagcagc tgcttgaaca gctgcgggct ctgcggcagc gcgtaccagg
      421 agcccggatg caggcgcgcg ggcaccacga agtcgcgggc accctcagga gtcgagcggg
      481 taagcgtcgg ggtttcgatg tcgtagaact cgagcccgtc caaggtctcc cggatcgccc
      541 gggtcaccgc cgagcgcagc accagcgcgt cgtgcatccg gccacggcga aggtcaaggt
      601 agcgatgctt cagccgggcg tcctcgttta cagtggtgcg ctcgtcgagc tggaacggca
      661 gcggcgcgga cgggttgagc acctcgagac ccgacgcggc cacctcgatc tcgccggtcg
      721 ccagcgccgg gttgaccgtg tcggccgagc gggcctcgac cacgccggtc accgagatgc
      781 agtactcgtt gcgcagcgcg tgcgcaccgg aggtctccag aatgtcgtcg cgaaccacca
      841 cctgcacgat gccgctggca tcgcgaaggt cgaggaaggc caccccgccg aggtcgcgcc
      901 gcttggccac ccaaccggcc agcgtgaccg gggtgccgac gtgttctggg cgcaggctgc
      961 ccgcgttgtg cgtgcgaatc acgaaaagtc ctttccgggg cccaactagg ccgaaggaga
     1021 tcttacgacg gacggacgca ggtccgcgtc cggcggcagc cagctgtcgg cgtcggcggg
     1081 cacctgctcg ccggagcgga tgtccttcac ctcaccgctg gcgaaccaga cgaacggaat
     1141 accgcgacgg tcggcgtagc ggatctgctt gccgaacttg tccgccttcg gcgccacctc
     1201 ggtggcgatg ccgcgcgccc gcaactgggc ggcggtggcc atggccgcga accgggattc
     1261 ctcgtcgtcg acggcgacca ggacgcagct gggcaccggc cgggacgcgg tcagcacgcc
     1321 cttgccgacc agcggcacca ggatgcggct gaccccgatg ctcaggccga cacccgggta
     1381 ggtggtgcgg ccgtcggagg ccagcgagtc gtagcgtccg ccggacgaga tcgagcccat
     1441 cgattcgtag ccggccatcg tggtctcgta gaccgtgccg gtgtagtagt ccagtccgcg
     1501 ggcgatcttc aagtcggcca ccagccgtcc ggggacgagg gtgttcgccg cgctgaccac
     1561 gccggccagc agttccaggc cctcggtcag cagcgggtgt tcaacgccca gcgcggtgac
     1621 ctgctcgacg aaggacgcgt ccgcgctgcg aacaccggcc agcttcaggc aggcgtcggc
     1681 ggccgccgag ctcaacccga cctcatcgac caacaacgta cggacggcgt ccggcccgat
     1741 cttgtcgagc ttgtccaccc gctgcagcac ggccagatgg tcctcgatac caagtccgcg
     1801 gtagaagccc tcgctcaact ggcggttgtt cacgctcatc gtggccggcg gcacgccgaa
     1861 gtcgacgtgc agcttttcca gcgcctccag ggtcaccagc ggcagctcga catcgtggtg
     1921 gccggcgagc gtctgcgagc cgacgatgtc gatgtcggcc tgggtgaact cgcggtagcg
     1981 gccctcctgc gggcgctcgc cacgccacgc cttctggatc tggtaccggc ggaacgggaa
     2041 ctgcaggtgg ccggagtttt ccagcacgta gcgtgcaaac ggaacggtca ggtcgaaatg
     2101 cagaccgagt tcgtcgcggg cacttgcatc ggcgtgcagc cggcggacga cgaagacctc
     2161 cttgtcgatc tcacccttgc gggtgagctg gtccatcggc tcgaccgcgc gggtctcgat
     2221 cgagccgaag ccgtgcaact cgaaggtctg ggcaagggag gcgagcacgc gctgttcgac
     2281 gatccgtccg gacggcgtga actcggggaa gccggacagt gctctgggtc gggacatcag
     2341 ttcctcagga aagcgggttg caggtaaggg ttctgggcac gctcggcggc catcgtggtc
     2401 tggtcgccgt gaccgggcag cagcgcggtg tcgtcgggca gggcgagcac tatgtcttgc
     2461 agggtgcgca gcatggtggc ctggtcgccg ccgggcaggt ccgtccggcc gaccgacccg
     2521 gcgaagacga catcgccgga gaacacgatc cggccgatcg gtggatggtc ggggtacggc
     2581 aggccgagca acaccgaccc ggcggtgtgc cccggagcgt gggtcacgct gaactcgagg
     2641 ccggcgagcg cgaccgagtc gacggccagc gagcggacgt ccgccggttc ggccagcggt
     2701 cctcccagca gctgctgcac cagatcagca ctatcggtgc tcagcccggc ggcgggatca
     2761 ctgagcagat ggcggtcggc cggatgcacc cagaccggca cctggtgcgc ctcggcgaaa
     2821 ttggcggcgc tggcaacgtg gtcgaagtgg ccatgggtca ggagcacccc cgcgggggcc
     2881 agccggtgct caagcagcac ttcctcgagc acttcggccg cgttctggcc cgggtcgatc
     2941 accacacagg tggtggcatc ggcaggtgcc accacgtagc agttggcctg ccacaggccg
     3001 ctggcgaagg aggctaggaa cacgtcccac accctatcga acgcccgcgg acggccagcg
     3061 ttgtcacaaa ccgcgccgaa tcgggcaaga tagcccccat gacttccacc tcaggtccgg
     3121 cggacttcgg gcgcgtcgac gctgacggca cggtttacgt gcgcacggcg gacggcgaac
     3181 gagccgtcgg tcagatccca gacgtcgagc ccgctgaggc aatggcgttc tacacccgcc
     3241 ggtacgaggc ccttgaggtt gaggtcgggc tgctcgaaac ccgggtgaag ggtggcgcac
     3301 tgtcgccgga ggaggccagg ggttcgatca ataccgttcg ccagaccgtc gggtcggcga
     3361 acgcagtggg cgacctggac gcgctgctgg cgcggctgga gagcctgcag ccgctgttgg
     3421 ccgagcaggc ggaggcccgc aaggccgagc gctcccgcca gcaggaagcc actcgggaga
     3481 ccaaggagaa gatggtcgcc gaggctgagg ctttggcgtt gggcaccgac tggcgcggcg
     3541 gggtcaaccg cttccgcacg ctgctggacg agtggaaggc gctcccgcgg atcgaccggg
     3601 ccagcgacga cgcgttgtgg catcgcttct cggcagcccg cacgacctac acccgtaggc
     3661 gcaagtcgca gttcgcggag caggcagcca agcgcgacgt ggcgaaggcc acgaaggaac
     3721 agatcatcgc cgaggcgcgt gaactcgcca agtcgaccga ctggggaccc accgcgggcg
     3781 cattccgcga cctgatgacc cgctggaagg ccgccggggt agctccgcga gaggtggacg
     3841 accagctctg ggccgagttc cgcggcattc aggacggctt ctttggcgcg cggacggccg
     3901 tgctgaccga acaggacgcc gagttcaagg cgaatctgga cgcgaagctg gtcctgctgg
     3961 acgaggccga agcgagcctg gtgcccgtca ccgatctggc tgcagcgcgc gctggtttcc
     4021 gcaccttcct ggagaagtac aacgcgtacg gccgggtgcc gcgtgagcag atgcgcagtc
     4081 tggacgcgag agtgaaggcg ctagagaccg ccgtccgctc tgccgaagag gatgagtggc
     4141 ggcgcaccga cccgcaggcc agagcccggg ccgaggagac cgtcggcatg ctcagcgccg
     4201 agatcgagaa gctcaccgag aaggtggcca aggcgagcgc tcgcggcgat gcgtccgcag
     4261 ccaagaaggc tcaggactcg atcgacacgt acacggtctg gctcgaccag gccaaggcga
     4321 cgctggccga cttcagccgc taacggcgcg ctccgtcggc agcaaccgcc acgcacgtgc
     4381 agggagcccg gcctacgccg ggatgacgtc catcccaatc attgggtcat cccgggcttg
     4441 acccgggatc tccgccggct acgccgggat gacgctcgca gagcgagcgt cagttcttca
     4501 gtcggaagac gtcgtagaca ccgggcacct tgcggacggc ctcgatcagg tgctccaggt
     4561 gggtggggtc ggtggtctcg aaagtgaacc ggaacttgaa gatgtggtcc ttggtcgtgt
     4621 tcagggtcgc ggactggatc gagatgtggt gctcccccat gatccgggtc acctcggaca
     4681 ggatgccggt gcggtccagg ccctcgacat gcatggccac caggaacgaa ctgctcgcgg
     4741 tcggcgccca ggacacctcg accaggcgtt cgggctcgct gcgcagggac tcggcgttgg
     4801 tgcagtcctt gcggtgcacc gacacgccgc tctcccgggt gacgaacccg agaatctcgt
     4861 cgcccggcac cgggcggcag caccgagcca gcttcaccca gacgttgggt gcgcccttga
     4921 ccaccacacc ggcctcgtcg ctggatgatc gcgtccgacg cggtcggctg atcaccgact
     4981 cgtcctcgcc gcgcaactcg tccactgcct cagcgccgcc ctcggacgtg atcagccgct
     5041 gcacgacggc ctgggcactg atcgaactct cacccagcgc cgcgtagagc gcgtcggtgt
     5101 cggccagccg gaagtgctcg accaggcccg acatgtgttc gggcgtgagc agccgttgca
     5161 gcggaagccc ggcccggcgg agctgcttgg tgagcaggtc cttgccgttc tcgatcgact
     5221 cctcgcgccg ctcccgcatg aagtgctgcc ggatcttgcc gcgggcgcgc gggctcttca
     5281 cgaaacccag ccagtcgcgg ctcggtccgg cattggcggc cttcgaggtg agcacctcga
     5341 cgacatcccc cgaagtcagc gcggattcca gcggcacaag ccggccgtta acccgggcgc
     5401 cgatggtgcg atgcccgacc tcggtgtgca cggcgtacgc gaagtccacc ggcgtggcgc
     5461 cctggggcag gttcatcacg tcgcccttgg gcgtgaacac gaagacctcg tcggtggcga
     5521 tctcgaaccg aagggtgtcg aggaactctc ccggatcctc ggtctcgcgc tgccactggc
     5581 tgatctgctg cacccagttg agctcggtgc cgtcagcttt gccctccttg tacttccagt
     5641 gggcggccac accgtactca gcctgtcggt gcatctcgtg cgtccggatc tgcagctcga
     5701 ccgccttgcc gcccggcccc agcacagtcg tgtgcaacga ctgatagagg ttgaacttcg
     5761 gcatcgcgat gtagtccttg aaccgccccg gaatcgggtt ccagcgggcg tgcagcgtgc
     5821 cgagggccgc atagcagtcg cgttgcgtgt cgaccaggat gcgcaacccg accaggtcgt
     5881 agatgtccgc gaagtcgcgt ccgcgcacca ccatcttctg gtagatcgag tagtagtgct
     5941 tcggccggcc gtagaccgtt gccttgatcc cggcctcagc caggtcagcg cgtacctgct
     6001 cggtgaccac gcgcaggtag tcctcccgca gcggtgcccg cgcggccacc agcttcacaa
     6061 tctcgtcgta gatcttcggc tgcagcgtgg cgaacgacag gtcctccaac tcccacttga
     6121 tcgcattcat gcccagtcga tgggcgagcg gggcgaagat ctcgagggtg tccctggcga
     6181 tggccatctg cttgtcctgg cgcaggtagc cgatcgtgcg catgttgtgc agccggtcgg
     6241 ccagcttgat caccaggacg cggatgtcct tgctcatcgc gagcaccatc ttgcggatgg
     6301 tctctgcctt cgccgactcg ccgtaggcga tcttgtccag cttggtgacg ccgtccacca
     6361 tcgcggcgac ctccggaccg aagtcggcgg tgagctgctg cacggtgtac gacgtatcct
     6421 cgaccgtgtc atgcagcagg gccgcacaca gcgtggcctc gttcatgccg agttcggcca
     6481 ggatcgtcgc caccgccagc gggtgggtga tgtaggcgtc cccgctcttg cgcagctggc
     6541 cggtgtggta gtgctccgcg gtgcggtagg cccgctcgat cagggcgacg tcagccttcg
     6601 gccggtgggt gttgacgatc cggagcaacg gatcgagcac cggggcgggc tggttgcgct
     6661 gcgcgccgat ccgcgccagc acctggcgca tgcgcatccg cggctggtcg gtggctgcca
     6721 aacgcgtccg cggttccccc ggcagcccat cgccttcgcc gtacggaccg tggatcggtt
     6781 gggtcacgcc acctcccgat tgatgacttg aggaaggctc actctactcg tggcgtgcgg
     6841 tgacctgggg ccctgcgcac cacgatcacc tcatcgccgg tacgcaactg gtcggcgctg
     6901 gcctcgtagt accgccgcaa ggtgcgattc cgcaccacac cgagcacctt ctcgccctgg
     6961 atctcggtga tgctctttcc gctctcctcg gcagtgatcg gacgctgggt cacttccagc
     7021 ccctgcccgg acgacagcag atcctcgatg atgtcgccaa ggtgcgggct gatcgagctc
     7081 aggcccatca gccggccgac cgcgtccgag gaggtgatca ccgcgtccgc acccgactgg
     7141 cggatcagcg gcacgttgtc cgagtcgcgg acgaccacca ccacatgggc gccgcgattg
     7201 agctgccgga cggtgagcgt ggtcaggatc gcagtgtcgt cacggccgac ggtgatgatg
     7261 atctcgcgcg ccttgggtag ttcggcgcgg tgcagcaact cgcgcgaggt ggcgtccccc
     7321 tcgaacgcgg ccaggccgtg ccggttggcg gcggcgacag cctgggggtc ggtgtcgatc
     7381 accagcgtcc gctccaccgg caccgagccg cgctgcaggg tggcggcggc gctctggccg
     7441 gtagtgccgt agccgatgat gacggtgtga ttgcgcatgg tcttcctcca gcgggagtcc
     7501 tgcagtgccc ggcggccttc gttggccagc acctcgacgg tggtgccgac cagcagcacc
     7561 aggaaggcga tcctgatcgg ggtcaccacg aagatgttga tgatccgggc ctgcacgccg
     7621 accggggtga tgtcgccgta gccggtggtg gtcatggtga ccgtggcgta gtacaacgca
     7681 tccacgaacg agatgccgtc ttgggcgacg ttgtcggagt acccgtcccg gtcgatgtag
     7741 acgatcaggg tgagcgccag cagcagtccg accccgacca acacgcgtag cagcaactgg
     7801 cgcgccgcgg agctggtcgt cgccggcagg tgcacctggg cgcgcccggt ccagggctgc
     7861 cgactgaggc cgatccgcgg ccggatgccg tccatcagac cgtgatcact gccgaagttt
     7921 cggtgatccc ggcgtccgcc aacagcttgc ggccaccgag gaagctgagc tccagcagca
     7981 ccgagacgtg gatcagttcg gcacccagct tctcgatcag cgccgcggta gcaccgatcg
     8041 tgccaccggt ggccagcacg tcgtcgatca ccagcacgcg cgatcccggc ggaatcgcgt
     8101 cccggtgcac ggcaagcgtc tcgtgaccgt actcgagcga gaacgtctgg gtgtagacgt
     8161 cgccaggcag ctttcccggc ttgcggaccg gcacgaagcc cgcgcccagc tccagcgcca
     8221 ccgggccggc gaagatgaac ccgcgagcct ccatgccgac caccacgtcg ataccggccg
     8281 gagccgtgtt cgcgagctcg gtgaccgcgg cccggtaacc agccgcgctc gagagcagcg
     8341 gggtgatgtc cttgaacagc acgccgggct tcggaaagtc ggggatgtcg cggatcaggg
     8401 acgcgatcag ctcccggttc tgaactgcgg ttggcgcggt cacttcttgc gctggcttct
     8461 gggctggcgg gtgggctgac gccgttcgcc ggaggcaagg tccttcgcgc tgaccaggcc
     8521 gagcgtggcc gggtcgacgg ctgcgacctc ggggtcgccg acggcgaccg tgctgaccgg
     8581 cttggccgtc ggcttgtgcg tggcacgctc cgacgtccgc gcggcgcggc gggcgagccg
     8641 ctcgcggtgc tccttcatgt ccgaatcggc ttcacgcatc tgcgccagca gcggcgtcgc
     8701 gatgaagatg gacgagtagg cgccggcgat catgccgatg aacagggcca ggccgaggtc
     8761 cttcagcggg ccggtaccga gtacgaacca gccggtgaac agcagcgccg cgaccggcag
     8821 tacgccgatg atcgtggtat tcagcgaacg caccagcacc tggttcagcg actggttcgc
     8881 cagcaccgag taggtgtgct tggtgttcgt gatgtccctg gtgttttccc ggatccggtc
     8941 gaacaccacc accgtgtcgt agagcgagta gccgaggatg gtcagtacgc cgatcatcgt
     9001 cgccggcgtg accgtgaagc cgaccagggc gtacacgccg acggtcacga tcaggtcgtg
     9061 cagcagagcc gcgatcgccg cgatgctcat cttgaagtcg cggaaatagg cccagatcag
     9121 cagcatcacc agggcaagga agaccgccag cgcgatcagc gcctgggtgg tgatctgctg
     9181 gccccaggag gcgccgatga gtgaatagcc cacctcgtcc ggctgggtgc cggccacggc
     9241 cgcgatcgca cccttcacct gggtgacctg ctcggtggtg agcgccttcg tctgcacgcg
     9301 gacggacgag tcgccgaccg tggtcacgct gatgtcgtcg gcgttcggca ggttcagctc
     9361 ggccaccgct gcgcggacgt cgtccactgt gttcgcggtg accttgatcg gtgcctggaa
     9421 gtcggcgccg ccggtgaact cgatgcccca ggtcgggccc ttgatcagca gcgcacccaa
     9481 tgacaccagc aacaacacag cggagatgag gtaccagcgc ttgcgcttcg ccacgaagtc
     9541 ataggagacc tcgccggtgt acagcttgtg cgccagcccg ctcttcttcg gcttctgcgg
     9601 ggtttcgatc acttcggtgc tcacgcctgt gctcccttgg ctacggcggc cgtacgccgg
     9661 gaattgtgca gcggcgggtt ggcgctgccc aggtgttcgg cttcgaagcc ggagaaccgg
     9721 tgcccttcgc cgaagaactt ggtgcgaccg agcagcgtca tcatcggctt ggtgaagaag
     9781 aacaccacgg cgatgtcgat cagcgtggtc agaccgaggg tgaacgcgaa gcccttgacc
     9841 gcgccgatcg cgatcacgaa gagcacgacc gccgatagca gcgagaccga atcggcgacc
     9901 agaatcgtct tcttcgacag cgtccagccg gtctcgatgg ccgagcgcag gctgcgtccg
     9961 tctcgtactt cgtcccggat acgttcgaag tagatgatga acgagtccgc ggtcacaccg
    10021 atcgcaacga tggcaccggc gatgccgggc aggttgagcg cgaagcccat gctcgcgccg
    10081 annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nacgatggca ccggcgatgc
    10201 cgggcaggtt gagcgcgaag cccatgctcg cgccgagcag cacgatcatc gcccaggaca
    10261 gcaggcctgc gaccacgagc gagagcgaca ccaccagggc caggccgcgg tagtacagga
    10321 tgctgtacag cagcaccagg ccaaggccga tcaggccggc gatgatgccg gcttgcagct
    10381 ggtcaccacc gagcttggcc gacacgttgt caacgctgga cacctcgaac gaaagcggca
    10441 gcgcaccata ggacagcacg ttcgcgagcg aggtggcact ggcctgagtg aagttgccgg
    10501 agatactcgc ccgtccgccg ctgatcgcac ccttggtcac ctcgggcgca gagatcacct
    10561 tgctgtccag cacgatgccg aactggttct gcgggctggt ctgctcgttc aggtgcgcgg
    10621 tgaccgtgta gaaacgggcg ccaccgttgg cgttgaactc cagggagacc tcgtagctca
    10681 ccgaattctg cggtacgccg gcggtcgcgc gggtgacgtc gctgccgttg atcagcaccg
    10741 ggccgagcag gtacttgtat agcccgtcct cgtcacaggc gatcagtggc tggtcggtga
    10801 cgtcagggaa cttgtcgccg cacttgaagt tcgcgaaatc ggtgttgtcg cgatcgctcg
    10861 gcgtccattg cagcagctcg gcgagctcct tggtctcgcc ggacggagcg gtcggacgcg
    10921 gggacggctc cggcgtcggc aactcgggca ggggccgctt gttagccgtc ggagacgcgg
    10981 acgccgaagc ggtcggctcc gtgccgggat cggtggtggc ggtttccccc ggctcggcgc
    11041 tcgcgctcgg atccggcacg gctgtgccac cggcggcctc ggccgcgtag accttgcgga
    11101 agtagagctg ggcggtggtg ccgaccatct tcaccaggtc gtcctgctgg acgttcggca
    11161 ccgagaccac gatctggctc tcgccagagg tggtcacttc ggtctcgccc acgcccatcg
    11221 agtcgacgcg ctgctggatg atgccgcggg ccagttcgag gctggtcggg tcaaccgccc
    11281 cgctgccgga cgtgttcgcc gcggtcagcg taatggtcgt accgccgcgc aggtccaggc
    11341 ccagtttggg catccacgaa cctgacaggg ccatcagccc gtacagcgcc gcggtgacca
    11401 gggcgaggat caacagcgtc cgcagcggat gtgtgtgatt cctacttgca gttgccactt
    11461 ttgcctcagg gatagatcag ttcttctggg tgtcggggtt ttcccacgtg atcggggcgg
    11521 gctcggtcac gtcctcggga tcggcgccga cgaccggtac gtcctcgacc ggcgtcgcgt
    11581 cctcgaccgg ggcaccgtcc tcgatctgga agggttctgc cacgacgcca tcggcgtcgg
    11641 cagcttccac gtccgcgtcg tcggagtact cgaactcatc ctcgaccgcc tgcgggctga
    11701 tcgcgcgctt gtcgatggtc aactccacgc cgggagagat ctcgagcaca gcctgcttct
    11761 cgccgaggtg cttgatggtg ccgatgatgc cggagaccgt catgatccgg gaaccgaccg
    11821 cgatcgcctc catgaggttc ttctgctcct tggcccgctt ctgctgtggc cgaatcatca
    11881 ggaagtagag cgctgcgccc atgatgacga tcaacagcac ggtatccatg gaggtttcct
    11941 tctctgaact cggggacggc tgcacgccga cgccgaagta gcctagcgcc gatgattgcg
    12001 ataacccatt cggcgggccg cgagcgcgcc caccgaaagc ctatccgtcg ctgaacaggt
    12061 ccggcgcagc aatcggcggc tggttgtggc cgggtggggt caacccgaga tgctgccagg
    12121 ctctggccgt tgccacgcgg ccacgcgggg tccgcatcag aaagccttcg cgcaccagga
    12181 acggctcggc aacctcctcc acggtctcgg tctcctcggc caccgccagc gcaagcgtgg
    12241 tgagcccgac cggtccgccg ccgaagtgcc gacagagcgc gtccagtacc gcccggtcga
    12301 gtcggtccag gccgagcggg tcgacctcgt acaactccag cgccttgcgg gccaccgacc
    12361 ggctaagggt gccgtcggcg cgcacctggg cgtagtcgcg gacgcggcgc agcagccggt
    12421 tggcgatgcg tggggtgccg cgggagcgtg aagcgatctc cttggcggcc tggccgtcca
    12481 ggtggatgcc gagcagagcg gccgagcggg tcaggatcgt ctccaggtcg ctggcagcgt
    12541 agaagtcgag ttgggcggtg aacccgaacc gatcgcgcag cgggcccggt agcagtcccg
    12601 cacgggtggt ggcaccgacc agggtgaatc gcggaatgtc cagcggaatg gcggtggcac
    12661 cggggccctt gcccacaacc acgtcgaccc gaaaatcctc catggccagg tagagcatct
    12721 cctcagccgg acgggacatg cggtggattt cgtcgaggaa gaagacctcg ccctcggtga
    12781 gcccggagag gatggcagcc aggtcgccgg cgtgctgaat ggccgggccc gaggtgatcc
    12841 ggatcggcgc gcccatttcg gccgcgatga tcatcgcaag ggtggtcttg cccagcccgg
    12901 gcgggccaga gagcaggacg tggtcgggca ccgcgtccct atgccgggcg ccggcgagca
    12961 ccagccccag ttggtcgctc acccgctgct ggccgcggaa ttcggccaat gaccccggcc
    13021 ggagggcgga ctcggcggcc tgctcgtcta cgaaggcgtg cgggtcgagc gggtcggatt
    13081 cgtcggtggt cacggcgctc actttgccag cgtgcgcagc gccgcgcgca acagcacggc
    13141 caccggcgtg tccggcgtct cctcggccag cgtcgaaacc cgatcacagg cggcctcggc
    13201 gtcccgggtg gagtatccga ggccctccag accggcccgc acctgctcgc gccaagcgct
    13261 gcccgcaatg gtggcgccca cgaccgtggc gccggtgccg agtgcggcca ccttgtcctt
    13321 cagttccagc accagtcgct gcgcgccctt ggcgccgata cccggcaccc gggtgagggc
    13381 ggccaggttg ccggacgcca ccgcagtgcg gaactcgtcg gggctgaata ccgacaccac
    13441 agcctgggcg atcttggggc cgatcccggt ggcgctctgc accagctcaa agcagtcccg
    13501 ctctcccggc tcggcgaagg cgtagagggt cagcgagtcc tcgcggacca ccagcgaggt
    13561 gtgcagcgtg accaactcac cgacccgcac cgaggtcgcg gtggccggcg tacacagtgc
    13621 cttcagcccg aagccggaca ggtcgagcac caaccaggtg ccaccgaccg cggccaccac
    13681 accggtcagc tgggcgatca tgcggtcctc ccggcagtct gcttggcgat ggcggcggcg
    13741 atccggtcgg cgccggcgcc gcgccaaagg tggcagatgg ccagcgcgac cgcgtccgct
    13801 gcgtcggcgg gcttcggtgg agcttcgagt ttgaggatgc gcgcgaccat ggcgcccacc
    13861 tgcgccttct cggcgcgtcc ggagccggtg atggccgcct tcacttccgt gggggtgtgg
    13921 gtgtggacgg ggatgcccgc gcgcgcggcc acgagcatcg ccagaccggc ggcctgtgcg
    13981 gtgccgatca ccgagagcac attctggtcg gcgaagaccc gctcgaccgc gatcgcgtcc
    14041 ggccggtgct ggttgaccca ttcggtgaga ccggcctcga tccgtaacag cctctggggc
    14101 aactccaggg tcgacggggt gttgaccacg ccgacggcca ccagctgcgg cggacgcccg
    14161 gggctgccgt cgatgacgcc caccccgcac ctggtcaggc cgggatcgac tccgagcacc
    14221 cgcatcgcga accttcccct agaacatttg ttcggatact agcccggtca agcccttgct
    14281 ccggccgtgc cacgcctacc aaccgcaaag tggacccgaa tcgtcgacta cggcgattcg
    14341 ggtccactct gcgttggttt gggggctcag gcgagctgct cgccgatctc gtcgggcaca
    14401 tcctcgttgc tgtacacgtt ctgcacatcg tcgaggtcct cgatcgcgtc cagcagcttg
    14461 aacagcttct cggcgatctc cgcggtgtcc acggccgtgg tgtactgcgg cacgaactgc
    14521 acctcggccg actcgtagtc gagaccggcc gcctgaacgg ccttgcgcac gtcgaccacc
    14581 aggctgggat cgctgatcac cttgaaggtc tcgccctcgt cggagacgtc ctcgggatcg
    14641 gcgtccaggg tcgcttccag caggtcgtcc tcggtgaggg tgcgtccggt ctggctcttg
    14701 gcgacctcga cgacaccctt gcggtcgaag atgcgcgaca ccgaaccgac gtcggccatg
    14761 gtgccgccgt tgcgggtcac cgccacgcgc acatcggagg cggaccggtt ccggttgtcg
    14821 gtgaggcact cgatcagcag gccgacgccg ccgggaccgt aggcctcgta catgatctcg
    14881 gtgtagtcga ccgcgtcacc gccgacgccg ccgccgcgct tgaccgcgcg gtcgatgttg
    14941 tcgaccggta ccgagttctt gcgggccttc tggatggcgt catagagggt cgggttcccc
    15001 gagggatcag cgccaccggt gcgagcggcg acctcgatgt tcttgatcag cttggcgaat
    15061 agcttgccgc gctttgcatc gatggccgcc ttcttgtgtt tggtggtagc ccacttggag
    15121 tgaccgctca tttacgtccg tccggtaaga agttggtttt gcgcgcctta gtctaggcca
    15181 cctcctctgc gaagctcgtt ccgcgcggct cgtcgtccga tcgacggggc tcgtgcccca
    15241 catcacgagt caagaccggc tggccacaat cggcgagagc cagcccctgg gatcgagcgc
    15301 cagcatccgt ggctggcggt cgtgatcgga ggctggcgct cgcgggaacg ggggtggctg
    15361 cagccctcgt caagactcct tgccggctca gccgccagcg cgagcgagtc tgctcctcgt
    15421 ctaccctgac ggcatgagtg agcggttgca tggcgagtac aaggtgcccg gcgggaagct
    15481 ggtggcggtc gacctgagcg tggtggacga gcgcctcgcg caggtcagcg tgagtggcga
    15541 cttcttcctc gaccccgacg aggcgctgtc ggtgatcgac cgatcgctgg ccgggctcag
    15601 cgtggagctc ggcgtcggcg aattggccgc acgcatcgac tccgatctgg cccgggcaag
    15661 cgcggacgga ttgctcgggg tcccggtgac catggtcggg ttcgactcca gggcgatcgc
    15721 catcgcagtg cgccgggcgc tgggccgatc gaccggctgg agcgatcaca tcttcacctt
    15781 cctcaatccg ggaccgctgc atcccgccga gcactgcgca ttggacgagg tgctcgcccg
    15841 cgagttggcc gccggacgcc gcgggccgac attccgcttc tgggaatggg acagcgcggc
    15901 ggtggtgatc ggttcgttcc agtcgctgcg taacgaagtg gacgccgaag ccgcggccac
    15961 ccacggcgtg gaggtggtac gccgggtctc cggcggcggc gccatgttca tggaggctgg
    16021 gaactgcatc accttctccc tggtcgtgcc ggccaccttg gtggacggga tgagctacga
    16081 ggcgtcctac gccttcctgg acgagtgggt gctggccgcg ctggacgaag tcggtgtgca
    16141 ggcgcactac gccggcctca acgacatcgc ctcccccgcc gggaagatcg ccggcgccgc
    16201 gcagaagcgc ttcgtcggcg gcgcggtgct gcatcacgtg accatggcct acgacatcga
    16261 cgccgacaag atgcttcagg tgctgcgcat cggccgggaa aagctgtccg acaagggaac
    16321 caagagcgcg aacaagcggg tcgacccggt gcggtcgcag acccacctgc cccgcgcaga
    16381 ggtgatggca tcgttcgaag ccacgttccg gaaccgctat cgcgtggtcg aggggcagtt
    16441 gagcccggcc gaactagcgg ccgtcaagga actgacccag accaagttca ccaacgtcga
    16501 ctggatcggc cgcgtgccct gagccggacg ctcacccggc cagcagttcg gctgcctgca
    16561 tggcgtcggc gatccgtacg aagtcgtcac gcgggatctc gaacatgccc cggcggaaca
    16621 ccatgcccca gtgcgcgggg tcgctgacga agccaagcgt gcccaacagc ggccggaccc
    16681 cggcgccccc acactcggcg aaccggacgc cgatgcgcca cgggcggctg ccgtccgcca
    16741 ctctggcctc gtacggctcg gcgccgacca ccgtgccaca agcggtgaac tgctgcagtg
    16801 gccgtccgtc cggatcgctc tgccgaggcg agtagcagac gacctgatca cccggattga
    16861 gcctggccaa cctcgaccgc acgctcggat tcgcctgtag atagccgccg gccacgtcgc
    16921 gccgcacctg gtcgaggctc accgtgttga cccagtaccg ggtcggattc aatgaacttg
    16981 aagttttcat ggagcgagtg tggacgccgc tactgacagt ttcagcggag cgaatccagc
    17041 gttggcgccg aaatgtccgg gatcgaaatg cgggctctca gcccagatgg agcctccata
    17101 gcccgcagaa ctatgcccga atgggtcgcg ccgggctcaa ctgcccgaag ccaaccaggc
    17161 cgcgccgatc aggtgccgcc cgcgacgaca gctatcggtg gatccggatt agccggcgac
    17221 ggccagcgcg tcggccaggg tgaagttgcc gacgtaaagg gcggtgccga tgatcgcgcc
    17281 ctcgaccccc tgtggcacca actcgcgcag ggcgcgcagg tcgtccaggg tggagatgcc
    17341 accgctggcg accaccttgg catcggtgcg ggcgcagacg tcggtgagca actgcaggtt
    17401 cgggccggtg agcatgccgt cactggccac gtcggtcacc acgtagcggg cacagccggc
    17461 cgcgttcagc cgatccagcg tctcccagag ctcgccgcct tcgcgggtcc agccgcgcgc
    17521 ggccagccgt gtcccgcgca cgtccaggcc gatcgcgatc cggtcaccgt ggtcggcgat
    17581 gatgcgatcg caccactccg ggtgctccag cgcggcggta ccgatgttga cgcgacgaca
    17641 cccggtggcc agcgcccgct gcagcgacgc atcatcgcgg atgccgcctg acagctcgac
    17701 cttcagcgcc atctttgcca cgatctcggc gatcacctcg gcattgctgc cacggccgaa
    17761 ggccgcgtcc agatcgacca ggtgcaacca ctcggcgcca ccgtcctgcc agcgcagcgc
    17821 cgccgccagc gggtcaccga agaccttctc cgagccggcc acaccctggg tgagctgcac
    17881 cgcctggccg ccctgcacat cgacggcggg taggagttcg agcctcatga ttccttcctc
    17941 aacgtgccca gccagttgcg cagcagccgc gcgccggccg ctccggactt ctccggatgg
    18001 aactgcgtcg accagagcgc cccctgttcg accgcggcga tgaaccggtc accgccgtgc
    18061 tcggcccagg agatccgggc ggttcccggc cagtccggca cctcgtggac gccgtaggag
    18121 tggacgaagt agaaccgctc gtgctcgaca ccggcgaaca tcgcggacgc ggtcggcggt
    18181 tcgaccctgt tccaacccat gtgcggcagc ggtacggcgg ccaactccgc cacgacgcct
    18241 ggcagcacgc cgaccccggg tgtcggctcg ccatgctcga cgccgagcgc gaacagcacc
    18301 tggtgcccga cgcagatgcc cagcaccgga cgcttcgccg ccagccgccg caggatcagc
    18361 tgcggcccgc cgactgacgc cagcccgtcc atgcatgccg cgaaggcacc cacgccgggg
    18421 acgaccaggc cttccgcggc caggcaggcg tccgggtcgg aggtcagcac cacctccgcg
    18481 ccggccgcaa cgagtgcgcg aaccacggaa tgcaggttgc cggagccgta gtcgaggacg
    18541 gctaccagcg ggtcacgaga ggtcacaggc ttcccttggt ggacggcacc tgaccggcaa
    18601 tccgcggatc gatggccacg gcatcgcgca gggcgcgggc cagcgccttg aactgcgcct
    18661 cgacgatgtg gtgcggatcc cgtccggcga gcacgcgcag atgcagggtg attccggcgt
    18721 tgatcgcgaa ggattcgatc acgtgctggg tcatcgaccc ggcatagggc actcccgagc
    18781 caccgatcag ggcgtactgc tgaccttcgg gctcgccgga gtacaccacg tacggacggc
    18841 cggccaggtc gaccacgcag tgcgcgaggg cctcgtccag cggcacggtg gcgtccgcat
    18901 agcgacgcac ccccgccttg tctcccaggg cctcggcgaa ggcctggccg agcacgatcg
    18961 cggtgtcctc aacgctgtgg tggccgtcga tgtgcacgtc cccggtggtg gtgatttcca
    19021 ggtcgatgag cgagtgcttg gccagagcgg tgagcatgtg gtcgtagaag ccgaccccgg
    19081 tgctgatcgt ggactggccg gtgccgtcga gattcagggc cacccgaacc tcggattcac
    19141 tggtcgaccg ggtgcggacg gcggtacgcg ggctcatctt tcctccatca cttcagccag
    19201 agcggcgtag aacgcctggg tctccgccgg tgtaccggcc gagacccgca ggtagccggc
    19261 cggaccggtc tcgcgcacga gcactccccg ctccagcagc cgttgccaga catcatgtcg
    19321 gtcggcgaac gggccgaaca aacagaagtt cgcgtcggag tcgatcacct cgacccccag
    19381 ttgaggcagc cgaaccagca tctggtcccg ggtcacccgc aattcatcca cctgggcaag
    19441 cagctctgcc gaatgagcca gcgcgacccg cgcgaccgcc tgggtctgcg cggacagatg
    19501 gtagggcagc cggacgatcc ggcacgcgtc cacgatcgca ggcgaggcgg ccaaatagcc
    19561 cacccgaccg ccagcgaggg cgaacgcctt gctcatggtc cgggacacca cgaggttgcc
    19621 gaagcgcggc agcagggtca gcgcggtctg ctcggggtgc cgggcgaact cctggtaggc
    19681 ctcgtcgacc accacgatgg cttcggtccc ggtcaacacc gcctcgacca cctcgagcgg
    19741 ggtcgtggtg ccagtggggt tgttgggggt ggtgatcacc accaccgcgg gctcatgttc
    19801 agcgatcgcc ttcagcaccg tgtccgcgtc caggctgtaa tccggcaacc gcggctcagt
    19861 gacgtaggtg gtgtgggtgt tgcgggcgta ctccggatac atcgagtagg tcggggtgaa
    19921 gctgagcacc ttgcgtccgg ggccgccgaa gccctgcagc aggtgggtca tcacctcgtt
    19981 ggagccgttc gcagcccaga tctgcagcgg gatcaggccg tggcccaggt agtccgccaa
    20041 cgcctcccgc aacgcccagg cctcgcgatc cgggtagcgg ttcagcgatt ccgccgcgac
    20101 cgccgacgcc atctcggccc ggatctccgg gccaggtggg tacgggttct cgttgacgtt
    20161 cagccgcacc ggaacgtcga gctgcggcgc gccgtagggc tcctccccca ccagttccgg
    20221 ccgcaacggc aactccccca ggttagcgtc gggcctcatg cgggccgcct cacggtcacg
    20281 gcggcgccgt gcgccgggag gtcctcggcc gcggcgaagg tctccaccac ccctgccacc
    20341 gccagcagcg cggcttcgga gtagtcaatc acgtgcacct gcttgcggaa gctgtgcacg
    20401 ttcagcccgg acgagtggca ggcgcagccc gcggtcggca gcacgtgggt ggagcccgcg
    20461 ctgtagtcgc ccagcgaaac cggcgagtac gggccgacga agatcgcacc ggcattccga
    20521 acccgcgcgg ctactgcggc cgcgtcggcg gtgtggatct ccaggtgctc cgcggcgtac
    20581 gcgtccacca ccgcgaggcc ctgcgcgaca tcgtccacca ggactatcgc cgactgccgt
    20641 ccggtgagcg cggtccgcac acggtcggca tgcttggtag cggcgacctg cggttcaagc
    20701 tcggcctgca ccgcctccgc cagatccacg gagtcggtga ccagcacgga cgcggccatc
    20761 gggtcgtgct cggcctggga gatcaggtcg gcggccacga acctgggcac tgcggtgcca
    20821 tcggcgagga tcgcgatctc ggtcggtccg gcttcggagt cgataccgat cagtccgcgc
    20881 agcaggcgtt tggccgcaac cacgtagatg ttgcccggtc cggtgaccag gtcgacgctc
    20941 gcgcagagcc cgggcacccc gtacgcgaac atggcgatcg cttgcgcgcc gccgacggcg
    21001 tacacctcct ccacccccag caatgcgcac accgcaagga tgttcggatg cggcaggccg
    21061 ccgaactcgg cctgcggcgg gctggcgacc gcgatcgagc tcaccccggc aacctgtgcc
    21121 ggaatcacgt tcatcaacac gctggacgcc agcggcgcga gcccgccggg cacgtagaga
    21181 ccgactcggc cgaccggcac gagcttctgc tcgaccaccg cgccgggtgc cagctccacc
    21241 cgggccggca cgctcaccgc ttcagcttcg gccaccagcc gacggcgacg gatcgcttcg
    21301 gtgaaggccg cgcgcacctg ctcgtccagg ccggaaagcg cgccggccaa ttcggcggcc
    21361 ggcactcgga aggagcgcgg cgtgacccgg tcaaaccgct cggagaactc ggcaagtgcc
    21421 gcctccccgc gctcggccac ggcagccacg atcggccgca cctgctcgac cgctgcgtcc
    21481 acgtcgaact cggcacgcgg caacacactg cggtagtcgg gcatggcccg gccacgcagg
    21541 tcgatagttc tcagcacaac cggattctat tgggcgggcg cacaccgacc gcgcgccgcc
    21601 cagcttcgtg ggcatcgtct gccgtgccag cgggtacgaa tgattgacta ggccgcatgt
    21661 tcatcggctt cggcaccgcc gtcaatgtcg tcacggtggt gatcggcagc gcactggggg
    21721 tgctggtcgg ccatcggctc ccccatcgca cccgcgagtt ggtcacccag gtgttgggac
    21781 tggtcacgct ggtgatcggc gggctgtcga tcgcgtccgg catgtcggag gccttcgccg
    21841 cagaggtcgg cgcccaggcc cggctgctga tcgtgctcgg ctcgctactg atcggcggca
    21901 tcatcggttc gctgctgcag atcgagaacc ggctcgaccg cggcgcccag ttgctgcacc
    21961 accggttcgc gtccggcagt gagggcagca ccttcgtcga gggcgcggtc accgccaccc
    22021 tcgtcttctg cgtcgggccg ctgtcgatcc tcggcgccct ctcggacggg ctcggccaag
    22081 gcgcggagca gttgctggtc aaggcggtga tggacgggtt cgcggccatc gccttcgctg
    22141 catcgctggg catcggggtg atggcctcgg tgatcccgct ggcgatctac caggggctgc
    22201 tgaccctgct ggggttcgcg ctcggcaact tcctgcccgt cggacaggtg gatgcgctgg
    22261 gctccgtcgg cggcgtgatc ctgctcggtc tgggcttccg cctggccggg atcaagcaga
    22321 tcccggtcgg tgatctgctg ccggcgctgg tcatggctcc cctgctgacg gccggggtcg
    22381 ggatgctggt ctgatggcgg gggccacacc ggcgatcgaa gcgctgctcg ctgccgggat
    22441 cgcgcacacc ctgcatccct acgaccacga cccgctggtc accgcctatg gcgatgagac
    22501 cgtggctgca ctaggtgtgg atccgctgcg ggtgttcaag accctggtgc tggacgtggg
    22561 cgccacccgg ccggagctcg cggtcggggt ggtgccggtg gccgggcagc tcgacctgaa
    22621 gcacttcgcg accgcgctcg gcgtgaagaa ggccgcgatg gctgcggccg acttggtggc
    22681 ccgaagcacc ggctacgtgc tcggcggggt ttcgccgctc gcccagaaga actcgctgcc
    22741 gacgatcatc gacgagaccg cggagctgtg ggacaccatc tatgtctccg ctggacgccg
    22801 cggcctacag gtcgagcttg cccctaacga tctcgccgcc gtggtgagcg cccgcttcgc
    22861 cgacattgcc cgctgagccg acaatgcgac ggttcaggga acgtccagag cgagcgcgtc
    22921 cgccagagct gcacggcccg cggccaggga ctcacgcagc agccgatcct cgtgctggcg
    22981 caggtcgtcg aacggcagcc gcggcaggcc gtccgccacc gcatgccagg cgacgtcgcc
    23041 gatcacctgc tgggcgacca cagcgtcctg caccgcgccc aggccctcgg tgaccgcttc
    23101 ccaacgggct cgccacacct cggcacccgc gcccagcact ggcacgagca gttcggagcc
    23161 gtaccgggct gccttcgcgg ccttgcgcac ctcgtgccag cgagtcaggt cggacggccg
    23221 ggacgcggcg tgctcggcca gcttgccgac ccgggccacc gcgcgtgcca gtagccgcgg
    23281 caacagttcc gcggcaggtc gctgagcagc cggcgaccat tgcggttcag ccagcagccg
    23341 gctcaaggcg agttggagtg cttcgtaccc cgcggtccgc atggacgcga ccagcttggc
    23401 gtgcgctgca gcgtgggtgc tccccagtga gcgctgcagt cgctcgccga ccagcaaccg
    23461 atcggtctcc ggaagctgcc ggacggcctc agcgagccgg gactgcagta cctcggcgtc
    23521 ccggggggca ccgagttcct cggcatgcca gcgcaactca ttccgcaagg tgccggtctg
    23581 gtcggcgaag acccgcgcgt acgtccgcag ggcgctgcgc aggcgccgcg tcgccactcg
    23641 gctgcgatgc accgcgtccg gtgcgtcgac cagcacgtcc gcttcgcggt tctgcagcac
    23701 gcccacctgc tcggcgaggt aggcatggac cgcctcgccg gcgctcgacc ggccgtcgag
    23761 cgcacgttgg gcagcgatcg cgtccgcgag tgcgcgtccg atcttcgagc cggattgcgc
    23821 ccggtcgatg ccatgcgcgg tcagtacggc ttcgagccgg tcgagcaggc ccaggtcgcc
    23881 gccgacaagc tcaatctcgg cctcacgcca gcgctcccgc cgagccccga cggacgcgac
    23941 cacgtcgtcg gtgcacgaca ccgcgagcac atcgccgcca gcgcccagca actgctgctc
    24001 acgccgattc gtccgcagca cggccaccgg caccagcggt gcaccagcga gtgtcgtggc
    24061 cacccggcgg cggaactcgt ccggcacccg gggcgctgcg ccatccgcgt gcacctcgat
    24121 gcgttgatcg gaagtgggtc cgggaagctt caggtgccag cccgcgtccg ctccgccgac
    24181 ccggtgccgg atcacctgcc aagcacgggt caaccgtaag tcgggcgcgt cgtagtaggt
    24241 ggcagtcaac tcgaactcgc ccaccgggcc aggagtgatc actgcggcaa ggtccggcag
    24301 ttcctgaccg gttgtgagcg catacttgcg ctcggcctcg atcagttggg ccacggtcag
    24361 ggcgtcgctt catcggtggc ggtgagcaca ccccgctcgt caacggtgcc ggcgtcctcc
    24421 ggttcgtcgt cggacgctcg ccgccaccag ttgtccgtgc cctcctcatc gtcggggccg
    24481 aaggacgagg ccagtagcac cggggtgacc gcggctagcc cccaaatcgc gagtgcggag
    24541 atcgagcgca gttccagcga cacgggcacc agatcgcccg cctgcgccgc gccgaggcga
    24601 tcggcgaagt tgttgggccc gatgagctcg ccgatctgcc agcacaccac tccggccagc
    24661 agaccggccg ccaccgcaag gagcgcggtc ggccagccga gcgcactgaa ccaacgccag
    24721 accaccagtc cgagtcccgc tccgaccagt gcaccggtca ccacgaacca gacgtccgcg
    24781 gccacgactt cagtcagccc acgctcagag atgctcgccg acccgtcctg ctgcaggatc
    24841 acgtagctcg gcaggtccgt cccgagcgac cagagcacac cggccaaggc accgaccacg
    24901 aggcagagca ctgcgagtag gccgatccag gaggccaccc cgcgcaccgg ttcgggcgcg
    24961 atggcctccg gcgacccctc gctgactgtc gtgctcacgc ggcagagctt acgcgttcac
    25021 gcacgacggc ccgagcagag ccttcaggtc ggccatcagc ggacgggacg gagcgacccg
    25081 cagaccgtcg tccagccgcc agatcttgct gccagcggtc gccgatacca gcttcacccg
    25141 cacctcggta gggccgggat gggcacgcag cacctgccgc aactgctcga ccaccgccgg
    25201 ggtacagcga accgccggaa gctggatcag caacgggccg ctcggcccct cggcgatgtc
    25261 cgggaaggcc acctcgttgc cctgcatctc caggctgtcg tccttggtgc ggatccggcc
    25321 cttgatccgc acgaccgtgt ccgtggcgag cgaagccgcc acgccggcat acaccttcgg
    25381 gaagagcagc acctccatcg acgactccag gtcctcgacc gtggcgatcg cccagaggtc
    25441 gccgttcttg gtctgcttgc gcaccacctg ggtgatcatg ccggcgatgg tcaccacgcc
    25501 gtcccgcggc ccgtcgtcgg cgcggagctg gccgatgctg tactcccggt gggcggccag
    25561 aatgtgctcc aggccctgca acggatggtc actaacgtac agcccgagca tctcgcgttc
    25621 gaagccgagc cggacgcgct tgtcccactc tggtaggggg ggaaccgggc ttgccgcgat
    25681 ctgctcggta ccgccgaaat ctccgaacag atcgtcctgt ccgttggcct ggttacgctt
    25741 gaggtcgatc acctcgtcca cgcggcgctc gaagcactcc atcagcccgc gccgggtgtg
    25801 ccccatcgag tcgaaggcac cggctttgat cagcgactcg atcacccgct tgttgcagac
    25861 cactagcggc acttggtcga ggaaagcgtt gaagtcggac gcctttccgc gctcagtgcg
    25921 cgcctcgacg atgccgcgca ccacgttgtc gccgacgttg cggatcgcgg ccaggccgta
    25981 acggatgtcc tgctcgactg gggtgaacat cccgtcggac gcgttcacgt ccggcggcag
    26041 cacgttgatg cccatccggc ggcattcgcc gaggtaaagc gcggtcttgt ccttgtcgct
    26101 cttcaccgat tccagcaggg cagccatgta ttcagccggg aagttggctt tgaggtacgc
    26161 cgtccagtag gagatcactc cgtaggcggc cgagtgcgcc ttgttgaagg cgtacgcggc
    26221 gaaggggacc atgatgtccc acagcgtttt gatcgaggcg ttggagtagc cgcgctcgcg
    26281 cataccggta ctgaagggct cgaactcggc gtcgaggatc gactgcttct tcttgcccat
    26341 cgcccggcgg agcaggtcgg cctggccgag gctgtaaccg gcgacccgct gggcgatctg
    26401 ctggatctgc tcctggtaga cgatcaggcc gtaggtggtg tcgaggatgt cggcaagcgg
    26461 ctcggccagc tccgggtgga tcggttccac cggctgccgt ccggtcttgc gaagggcgta
    26521 gttggtgtgg ctgttcgctc ccatcgggcc gggacggtag agcgcgatcg tggccgaaat
    26581 gtcctcaaag ttgtccggac gcatcatctt cagcagcgac cgcaggccgc cgccgtccaa
    26641 ctggaacacg ccgagggtct cgccggaggc cagcagttcg taggctgccg ggtcggacat
    26701 gtcccggcgc agtgcgtcca ggtcgatgtc gacgccgcgg ttggccttca cgttacggac
    26761 ggcgtcgtcg aggatggtca ggttccgcag gccgaggaag tccatcttga ccaggccgag
    26821 atcctcgcag ctcgggtagt cgaactgggt gatgacctgg ccgtcctgct cccgcttcat
    26881 gatcgggatg acgtcgagca gcggtgcgct ggacatgatc acgccggccg cgtggacgcc
    26941 ccactgccgc ttcaaccctt cgataccacg ggcggtgtcg accaccagct tcgcgtccgg
    27001 gtcggattcg tagagctcac ggaactcggt gccctcgccg taccggtcgt gctcgggatt
    27061 gaacacgtcg gccagcggca cgtccttgcc ctgcaccggc ggggtgtaag ccttggtcag
    27121 cttctccccc agtgagaacg gcatacccag cacgcggccg gcgtccttga tcgcctgctt
    27181 ggccttgatc gtgccgtagg tgacgatctg gcagacccgg tcgtcgccgt acttctcggt
    27241 gacgtagcgg atcacctcac cccggcggcg atcgtcgaag tcgacgtcga agtcgggcat
    27301 cgacggccgc tccgggttga ggaagcgctc gaagatcagg ccgtgcggga cggggtcgag
    27361 gtcggtgatg cccatggcgt aggcgcacat cgaaccggcg cccgagccgc ggccgggccc
    27421 gacccggatg ccgttgcgct tggcccagtt gatgaagtcg gcgaccacca ggaagtaccc
    27481 ggcgtagccc ttgccgatga tcacgtcctg ctcgtaggcg gcctgcttgg tcgcgtactc
    27541 gggcacatcg ccgcggaacc gtcgctccag cccggccgcg acctcacgct cgaaccagct
    27601 ggactcggtt tcgccagccg gtacggggaa gaccggcatg taggtgccgc tgccctcgtc
    27661 gaacgaggtc tcacagcgct cggcgatcgc cagggtgttg tcgcaggcct caggcaggtt
    27721 gctgaacagc gcacgcattt ccgctggcga cttcaggtag tagccgtcgc cgtcgaactt
    27781 gaaccggttc ggggtgtcct tgttcgcgcc cgcagacacg cagagcagag tgtcatggtc
    27841 ggacgcgtcc gaggcgtgca cgtagtgcag gtcgttggtg gccaccagcg gcagctgcag
    27901 atccttcgcg agccgcagca ggtcggcgcg tacccgggtc tcgatgtcga ggccgtggtc
    27961 catcagctcg acgaagtagt tctgcgcgcc gaagatgtcg cggaactcgg ccgccgactc
    28021 cctggcctgg gcgtactgcc cgagcctgag gtaggtctgc acctcaccgg acggacagcc
    28081 ggtggtgcca atcagcccct tggcgtagcg gttgagcagc tcccggtcgg cccgcggctt
    28141 gtagaagaag ccctccagcg agctcagcga gctcagtcgg aacaggttgt gcatgccctc
    28201 gttgtgctgc gcccagatgg tcatgtgggt gtaggcgccc ttggccgcga cgtcatcgcc
    28261 ggtgccgtcg ccgaactgca cgcgcttgcg ctcgcttcga tgcgtcttcg gggtgagata
    28321 ggcctccaac ccgatgatcg gcttgaccga gctgcccttg gccgccttgt agaactcgta
    28381 ggcgccgaac atgttgccgt ggtcggtgac cgccagtgcg gacatgtcga gttgctcggc
    28441 gcgggaaacg agttgcttca gccgcgccgc accatcgagc atcgaatact cggaatgaca
    28501 gtgcagatga acgaactctt cagccacgcg gacccacttc taaccaaacc gtgaagaaca
    28561 ccggaccgct gggggaagtc agcgagtaca caccataacc cgcgacgggg tcgatcctgg
    28621 ttcccgactc accggtgccg agagcccgac cagagtcggg acccgttccc ggcatcgctg
    28681 tcgttgcggg tgctgaggta gcgctcgatc ccagccgctt tgccgagctc gcggtgggta
    28741 cggtcgaacc ttgaacacca gccatgagca gcgcgatcgg atcgcgcggc aggcattcaa
    28801 gaacctcggg cagagcccga gccgcaaggc tgagctgacc gttcagtgcg actcttcgca
    28861 ccacctcgcc ggcgtctacg ccaccgacgt ggggctcgtg ttccactcgg tgctgcacgc
    28921 cagagcccgc gggcgtcgcg actatgccga cgtcgggcac cacgcttcac gcctggggct
    28981 cgactggttc gaccttctgg atcccccggc gcacgcgggc gtccccgacg aactttcggc
    29041 gggttgcgag tgcggcccgt acccgctgtc gcgcgcgctc ctgccccagc agatcgcggc
    29101 gggcgcgaag acggtcatcg tcccctgatt cgacggatgc gctcggaccg gagcgcctgg
    29161 tttgcccatg gccaccaggc gtggttgcat cggccttgat catcggaagg ggttaggcgt
    29221 gcaacaggtt tcgctcatcg gggcacccac cgacataggc gcttcccgca agggttgccg
    29281 gctcggtccg caagcactgc gggtggctgg catcggcacc gcgttgcgga agttcaactg
    29341 cgaagtgaag gacaccggcg acctgacggg cccgcccaat ccggagagcc cgcccgtcaa
    29401 cggctaccgc cacctggccg aagtgatcga ctggaaccgc tcggtgtacg cggccacggc
    29461 cgccgagttg gcagacggac ggctgccgat cctgctgggc ggcgaccact gcctggccat
    29521 cgggtcgatc agcgcggtcg cggcgcattg ccgggccacc gggaagaagc tgcggatcat
    29581 ctgggtggac gcgcacgccg acttcaacac cgctgaactc acgccgtccg gcaatatcca
    29641 cggcatgccg gtggcctgcc tcgcgggcca cggaccggac gaactgacca cgctgggtgg
    29701 atcggctccg gcgatcagaa ccgccgagat ccggcaggtt ggtctgcgca gcgtcgatcc
    29761 gggcgagaag gcgctgctct tcgcggaagg cgtcgaggtg ttcgacatgc gcttcgtgga
    29821 cgagatcggc atgcgccgga cgatgacccg cgccctggcc ggcgtggacg aggacaccca
    29881 tctgcacctg agccttgacc tcgacttcgt cgaccccgag atcgcgcctg gagtgggcac
    29941 cccggtggtc ggcggcccga cctatcgtga ggcccagttg gtgatggaga tgatcgccga
    30001 caccggccgc ctggcctcga tggacctggt cgaactcaat cccgcccttg acgtccgcaa
    30061 cgccaccgct gagctcgccg tcgacctggt cgagtccctg ttcggcaaga gcaccctgca
    30121 ccgcctgcac gagggcatcg ccctgtagct gtcgaacgat cccggagctt gacgaagggt
    30181 cgcgggggct tcataaggct cagccaccgt actagctcag ccaccgtccc tggttcagct
    30241 ggggtcttcg gcgggctcgg ggtcggcggg tggacgctcc aggtagctgc tcggggcgag
    30301 tttgccggcc acattcagat tggtgtcggc gctgggggcg atgtcggtgt tctgccagct
    30361 gaccgcgatc cagctgtcgt cggcgaggcg ttgcaccacc acggacgcga taccgcgccg
    30421 agcctgggcg ggctgccctt ccgggtcgac ctggccggtc atccgccagc gagcatggac
    30481 gagcgctgcg tcctccccca gccagcgcaa cttgagcttc tccacgacca actcggtggc
    30541 gccgaacatc cgctcgaagc cgcgcttgtg tgccctgcgg atcgagcgtg ggctcgtcca
    30601 ccacaagccg accacgttga cgaagtcgac atcatcggtg aacagttcag cgatcccgtc
    30661 cgcgtcgtgc cggttccacg ccttggtgaa cctccggact acctccaacg gctcccgcac
    30721 cggcgacccg cctgcgggta gcggcaatcg caccgccgag gtcgccagct ggcgcagttt
    30781 cttgaagctc accgaagact tgggtgacat gttcacccat cttacccagg ggcccatccg
    30841 cagctttgct cccgaatggg acttttgcca ccgataattc gggagcaaac gtccctttcg
    30901 ggagcgaagt tgaggcgggt gggcccagcg aagccgacga gcgggtcccg ccaggctcga
    30961 ccagccgatt tggttgaccg gcctagacca gcagcggacg atcggtcggc aggatcggct
    31021 tcggaagccg cgaactaccc tgcaggaacg cgtccacgcc gttggcgcag gcacgcccct
    31081 cggcgatcgc ccagacgatc agcgattggc cgcggcccgc gtccccacac acgaagaccc
    31141 gatcggccgt ggtggcaaag tcggcgttgc ggacgatgtt cccgcgcgga tctagttcca
    31201 agccgagctg ctcgaccacg ccggtgcgct gcgggccggt gaagcccatc gccagcagca
    31261 ccagttgggc cgaaagttcc tggttgctgc cgggcaccga ctcgaagcgg ccgttctcca
    31321 ggcgcacctc gttcacctcg aggccggtca cgttgcccga ctcgtcgccg ttgaactggg
    31381 tcgtcgaaac cgagtagacc cgctcgccgc cctcctcgtg cgcggacgag acccggtaga
    31441 tcatcggata ggtaggccac ggctgattca ccggacgctc ggtggacggc gtcggcagga
    31501 tctccagctg ggtcaccgac cgggcgccct gccgcaagga ggtgcccagg cagtccgcgc
    31561 cggtgtcgcc accgccgatg atcaccacgt ccttgccggt cgccaggatc tggtcgtcga
    31621 gctgctgtcc ggccgcgacc cggttggcct gcggcaggaa ctccatcgcc tggtggatcc
    31681 cggccagctc gcgaccaggg atcggcaggt cccgcgctac cgtggagccg accgccagca
    31741 cgaccgcgtc gaaacgctcg atgagctgct cgccggtgat gtcggtgccg atatccacgc
    31801 cggtcttgaa gaccgtacct tccagccgca tctgcttgag ccgctggtcg aggaccttct
    31861 tctccatctt gaactcgggg atgccgtagc gcagcaggcc gccgatcgcg tccgcccgct
    31921 cgtagacgac cacggtgtgc ccagcccggg tgagctgctg cgcgaccgcg agccccgccg
    31981 ggccggagcc gaccaccgcc accgtccgac ccgtgtgcca ggccggcgcc tccggctcga
    32041 cgcgacggtc gtcccaggcc cggtcgatga tcgcgacctc gacgttcttg atcgtcaccg
    32101 gttcgcgatt gatgccgacc acacaagccg tttcgcaggg tgccgggcac agccgtccgg
    32161 tgaactcggg gaagttgttg gtcgcgtgca gccgctcggc cgccccacgc caatcgtccc
    32221 gccagatcat gtcgttccac tccgggatca ggttgcccag cgggcagccc tgatggcaga
    32281 acggaatacc gcagtccatg catcggccgg cctgcttgcc gatgatcggc agcacagcct
    32341 tgcccggagt gcccgggtag acctcattcc agtcctgcac gcgctcggca accggacgcc
    32401 ggtcagcgac cttccgcggc gtggtcatga agcccctggg atcagccatg ggacgcctcc
    32461 atcatcagct tggtggtggt ctcctcgtcc aacccggcca gttcggcctc ggccctggcc
    32521 ttgagcacgc gggcgtagtc ccgcggcatc agggtggtga agcggccacc gagttcttcg
    32581 tcggacagct gcagaagccg gtcagcgacc gaagattcgg tctcgtcacg atgcttggcg
    32641 agcagctccc tgatccgggc cagctcagca gcgttcaccg gcagtgcgtc caccagttcg
    32701 gtgttcagcc gggccggcac cagatcgagc acccaggcca cgccgccgga catacccgcg
    32761 gcgatgttgc gtccggtggc gccgaggatc agcgcctcgc cgccggtcat gtactcgcag
    32821 gcgtggtcgc ccacgccctc gaccaccgcg gttgccccgg agttccggac gcagaaccgc
    32881 tcgcccacct gaccgcggat gaacagctcg ccgctggtgg cgccgtaggc gatcacgttg
    32941 ccggcgatga tctgttcctc cgcggcgaac ggcgcccgct cgtccggacg gacggtcagc
    33001 cggccaccgg agagaccctt gccgaagtag tcgttggtgt cgccgaccag ccggagggtg
    33061 atcccgcgcg gcaggaacgc gccgaagctc tggccggccg tgccgcgcag ggtgaagtcg
    33121 atcgtgttct ccgggagccc aacgccttga gtggccttgg tcacgtgatg gccaaggatc
    33181 gtgcccaacg tgcgatcgac gttgcggacg gtgaggtcgg cccgcaccag ctcaccgctg
    33241 gtgagcgccg gctgcgccat cgccaacagc tgggtgtcca tcttgtcgtc gaggccatga
    33301 tcctgggtgg tgacgcagtg cagcggggcg ccctcgggaa gctccacctg gtgcagcacc
    33361 ggggataggt ccaacccgcg ggccttccag tggtcgaccg cgcgccgagc attcagcacc
    33421 tcgacgtgcc cgacggcctc ttcgatcgtg cggaagccga gcgcggccag gtgctcacgc
    33481 acctcctggg cgatgaactc gaagaagttc acgacgaagt ctgcgtgccc ggagtacatc
    33541 gcgcgcagct cggggttctg cgtggccacg ccgaccggac aggtgtccag gtggcagacc
    33601 cgcatcatca cgcagccgga aaccaccagc ggcgcggtcg cgaacccgaa ctcctctgcg
    33661 ccgagcaacg cggcgatcac cacgtcgcgt ccggtcttca actggccgtc gcactggacg
    33721 acgatccggt cgcgcaaacc gttcagcagc agggtctgtt gagtctcggc gaggccgagc
    33781 tcccaagggc cgccggcgtg cttgagcgag gtcagcggag ccgcgccggt gccaccgtcg
    33841 tggccggaga tcagcaccac gtccgccttc gccttggaga cgccggcggc gaccgtgccc
    33901 acgcccacct cggccaccag cttcacgtgg acgcgggcct ccgggttggc gcacttcagg
    33961 tcgtggatca gctgcttgag atcctcgatc gagtagatgt cgtggtgcgg cggcggcgag
    34021 atcaggccga cgccgggcgt cgagtgccgg gtcttggcca cccatgggta caccttttgg
    34081 ccgggtagct ggccgccctc accgggcttc gcgccctgcg ccatcttgat ctggatgtcg
    34141 gtggcgttgc tcaggtagtc gctggtcacg ccgaagcgtc cggatgccac ctgcttgatc
    34201 gcactgcggc gctccgggtc gtagagtcgg tcggcgtcct cgccgccctc accggtgttc
    34261 gacttggcgc cgagccggtt catggcgatc gccagggtct cgtgcgcctc ccggctgatc
    34321 gagccgtagc tcatcgcgcc ggtggagaac cgctttacga tctcgctgac cggctcgacc
    34381 tcgtcgatgt cgatcgccga acggtcgctg ttgaactcca gcaggccgcg cagcgtcatc
    34441 agtcgttcgg actggtcgtc gacgcgtgcg gtgtactgct tgaagatgtc gtagcgccgc
    34501 tcgcgggtgg agtgctgcag ccggaagacg gtctccgggt cgaacaggtg tggcgggcct
    34561 tcgcggcgcc acttgtattc gccgcccccc gacagggtgc ggtgcggcag cgggatgccg
    34621 tccggcgggt aggcgcgggc gtgccgcttg gccacctctt cggcgatctc gtccaaaccg
    34681 atgccctcga cccggctggt ggtgccggtg aagtacttgt ccaccagttc gctggacagg
    34741 ccgagcgcct cgaagatctg ggcgccggtg taggacgcga tcgtggagac gcccatcttg
    34801 gacatcacct tgagcacgcc cttgcccagc gacttgcgga cgttgtagac cgcctgctcg
    34861 ggctccacgt gcaccagcca ctcccgccgg gcgagatcct cggcggactc gaaagccagg
    34921 taggggttca ccgccgcggc gccatagccg atcagcagcg cgacgtggtg cacctcgcgg
    34981 acgtcgccgg cctcgacgat caggccgacc tgagtgcggg tcttctcccg caccaagtga
    35041 tggtggatcg ccgcggtcag cagcagggac gggatcggcg ccagttcggc gttggaatgc
    35101 cggtcggaca ggatcagcgt ccggcagccc cgggcgatcg cctcgctgac ctgctcgcag
    35161 agctcatcca ggcgggcctt gagcgctgct cccccgccct cgacgtcgta gaggccgcgc
    35221 acaacatggg tgtcgaaggc cggcatcgag ccgtcgcggt tgatccgcac cagcttcgcc
    35281 agctgatcgg aatcaaggat cgggtagccg atcacgatct ggcggcagga ggcctcgctc
    35341 ggctcgagca ggttcgcctc cggtccgaag gtcgcggcca gcgaggtgac cagttcctcc
    35401 cggatcgcat ccagcggcgg gttggtcacc tgggcgaaga gctgcgcgaa gtagtcgaac
    35461 agcgtccgcg gatggtcgga caacaccgcg accggggtgt ccgagcccat cgagccgatc
    35521 ggctcgacgc cggtgttggc catcggggcg aggatcatcc gctgctcttc gtgggtgaac
    35581 ccgaagacct gctggcgccg cgtgaccgag gcgtggctgt gcacgacgtg ctgctgctcg
    35641 ggcaggtcat cgaggtcgac tcgaccggcc tcgagccagt cggcgtacgg atgctcggcg
    35701 gcgagctggg tcttgatctc ctcgtcgttg atcaggcggt gctgctccag gtcgaccagc
    35761 agcatgcggc ccggcttcat ccggcccttt cgcaccacct tggccgggtc gatgtccagt
    35821 acgccggctt cggacgcgaa gaccaccagc ccgtcgtcgg tcacccagta gcggcccgga
    35881 cgtagcccgt tgcggtcgag cacggcgccg atcaggctgc cgtcggtgaa ggtgaccgag
    35941 gccggtccgt cccatggctc catcagcgag gagtggaagg cgtagaaggc ccggcgggcc
    36001 gcgtccatct cggtgtggtt ctcccacgcc tccgggatca tcatcagcac cgcgtgcggc
    36061 agcgagcgtc cgcccaggtg cagcagttcg agcacctcgt cgaaggacgc ggagtcggag
    36121 ccgccggggg tgcagatcgg gaagatccgc tcgatgtcgc ccgggatcac ctcggacgcg
    36181 agcagcgcct cccttgcccg catccagttg cggttgccct tgactgtgtt gatctcgccg
    36241 ttgtgggcca tcagccggta cgggtgggcg agttcccagg ccgggaaggt gttggtggag
    36301 aagcgggagt ggaccagggc aagggcactc tcgacgcgct cgtcgagcag gtcggggaac
    36361 acctcggcga gctgctgggt ggtgagcatg cccttgtaga cggtggttcg tgaagaaagc
    36421 gacgcgaaat aaaccccggc gtcattgcgg gcccgctggc gcagggggta cacggcgcgg
    36481 tcgaggtcga ttccggcgag cagcgactcg gtggagacga agacctgctc aaagtgaggc
    36541 atgttgtgcg ccgaaaccgg gctgagggtg gagtcgtcgg tgggcacctg acgccagccc
    36601 tggacgacca ctccctcctc ggttgccagg aactcgaagt tgcgcttggc ggacgcgcgc
    36661 ttggtgggct cctggggcag gaaggccatg ccgaccgcgt attgacccgg ttcgggcagg
    36721 tcgaagtcga ccacctggcg gaagaagcga tcgggaacct gcagcagcat gccggcaccg
    36781 tcgccagcgg cggcatcggc gccggtggca ccacgatggt cgaggttctc cagggcggtc
    36841 agaccctggc tgacgatgtc gtggctcggc gccccgctca gctgggcaac gaaggcgacg
    36901 ccgcaggcgt cgtgctcgta tgccgggtcg taaaggccct cggccgggtg gggtggcatc
    36961 gccagcttcg ccatatgtat ctcccatctg cgcgtactca gcaggggcgg gtgcagacca
    37021 atcagactgc acgtcgacgc tgcacgctaa ggcgcgagcc tacctgacgt ctgcccattg
    37081 tctggacaag ctgactgcat cttggacgcg gtgtctcgat cctcggcaaa gctcagggca
    37141 ggcgagctcg accagccggt cgcgatcgaa cgcaggacaa gctgccgagg attgtcaggg
    37201 tcagtccttc gtatccggtg acgaggttga cggcgaccca tcggggtcgg cccctggctc
    37261 ctcggcgggt tcctcgacct gctcatcggc cggtcggtcc ggtcgagacc cgtcggccgc
    37321 tccaaactcc acgacctcct cgcgtccggg gcggaaccgc accagccaga tcagggcggc
    37381 cacgccgatc aggcagatca ggatggcgac ggcctcgttg aagcggagcg agccgaaggt
    37441 gtcgtaggag tagtcgagcc ggatgtgttc ggtgaagatc cggccgaagc cgtagagcgc
    37501 cacgtacagg gcgaagacct tgccgcggcc cagccggaac cgcttgtccg cccacagcag
    37561 cgtgcccatc acgaacaggt tccacagcga ctcgtaaagg aaggtcgggt ggaaggtctc
    37621 gtactgctcg aagccgggag cccggtgcgc ggggtcgatc tccaggcccc agggcagcgt
    37681 ggtcggctgg ccgtacagct cctggttgaa ccagttgccg aaccggccga gagcttggcc
    37741 gagcgcgatg ccgggcgcga tcgcgtccgc cagcgccggg aaggagacct tctgccgccg
    37801 ggaggtcagc caggccacca gcgcgcccgc acccaccgca ccgtagatgg cgatgccacc
    37861 ctcccagatc gcccaccagt gctgggtggc gccggcaccg aagtagtcgc cgaggtgggt
    37921 cagcacgtgg tagatccgcg cgccgacgat ggcgagcggg atcgtccaga gcagcacggt
    37981 ctcgaacgac tcgctggtgc cgccgcgcgc ccgccagcgg cgcacgccca gcgtccagcc
    38041 ggcgaagatg ccggcgatca gacagagcgc gtagatgtgg atggtcagcg ggccgatctg
    38101 gaagttgctg atcgacgggc tcggaatgaa caaggtcacc aggccatcct tatcgctggg
    38161 tcaaccctgt tgtcgaatca gggtctctag gtcggctttc agcacgccga tcgcggtgcc
    38221 ctgcggccac accagcacgc cgcgacgctc ccggtcgaag ccgacgatct gggcagtgtg
    38281 attcacctca tagccggtcg cggccttctc gatgccggcc acacccaccc ccatgctggc
    38341 catcagctgg tcgagatcgg cctgctcgcc ggtcaggccg atgaagtccg ggtcgatccg
    38401 ggagagatac ttcgcgagca ccgccggagt gtcccgcgcc gggtcgacgg tcacgaacag
    38461 cacctggacg cgcgcacggt cggcgtcgct gagccggttc atcgccgagg cgatgtcggt
    38521 gagcacgccc aggcagatat ccgggcagtt cgtgtagccg tagtagaaga ccagcaccgg
    38581 cctttcgctg ctggtgcgga cgttgaagtc cccgccggtg tggtcggtga gcgtcaggtc
    38641 gggcagggcg tagggcgtgg tcagttccac accctggaac ctgctcgcac tctcgctggc
    38701 caccgggacg gacggcgttg cgggcgcggt gcagccggcc aacagcgcta gcgccagacc
    38761 ggctccggcc aacgccgacc tcatgcccga cgaacgccgg cggtcagatc ggccagcacg
    38821 gtccgcagcc cggaaaggtc gtccgggttg ccggcctcat cggcggccaa cagtggcttg
    38881 atgaacgccg aaccgacgat caccccgtcc gcgaagccac agacctcggc ggcctggtcg
    38941 ccgttggaga cgcccagccc cacgcccacc agtgtctctg gagccagcga ccggacgcgg
    39001 gcgaccaggt ccggcgcggc actcgaactg gcgctgcggg tgccggtgac ccccatcacc
    39061 gaggtcgcgt agagccagcc gcgcccggcg gcgaccgtgc tcacgatgcg gtcgtctgat
    39121 gacgacggcg aaaccaggaa cacccgatcc aggccgtagc ggtctgatgc ggccagccag
    39181 tcgtcggctt cgtcgggaat gagatcaggc gtgatcaggc cggagccgcc agcgttggcc
    39241 aagtcgcggg cgaaagcgtc cacgccgtag cggtcgacca ggttccagta ggtcatcacg
    39301 accgccggcc gacccgcgct acgcacggtc tccaccgcgg tgaacacgtc cttggtgcgg
    39361 acgccgcgct ccagcgcccg ggtgccggcc cgctggatga cggggccgtc catcatcggg
    39421 tcggagtagg gcaggccgat ctcgaccagg tcgacgcctg gcgtctcccc gtccgcgccg
    39481 cagagcgtgg ccagcgcggc cattgaaccg ggcacggaag ggtaaccgac cggcaggtag
    39541 ccgaccagcg cgccgcggcc atcggccctg gccgcggtga acgccgcagc cgagctttga
    39601 acggaaacgg aaccgtcccc gttttcgtcc aggttcgccg gggtttcgac aggctcaacc
    39661 acggtggatt cgctcatgcc acgcctcccg tcgctgcgtc cggtttgccg gtgagaccga
    39721 aatacttgat cgcggtgtcc acgtccttgt cgccgcggcc ggacaggcac accacgatga
    39781 tcggctcggt ctccggatcg gtctgggcaa ggcgctggcc gagccgcatc gcgccggcca
    39841 gggcgtgcgc cgactcgatc gccggcatga ttccctcggt gcgggtgagc aactgcaggg
    39901 cggtcaacgc ctcggcgtcg gtcaccgcct catagttggc gcgaccggtg gccgccagcc
    39961 aggcgtgctc cggcccgacc cccggatagt ccaggccggc cgagatcgag tgcgactcca
    40021 gcgtctggcc gtccgcgtcc tgaaggatgt gggtgcgcat cccgtgcagt acgcccaccg
    40081 aaccgcccag gatggccgcg gcgtgccgcc cggtctccac cccgtcaccg ccggcctcga
    40141 agccgtagag cgcgacctcg ggggtgtcga tgaactcggc gaaggtgccc atggcgttgg
    40201 aaccgccgcc aacacaggcg gccaccgcat cgggcagccg gccgtagcgg ccgagcatct
    40261 gttcacggat ctccgcgctg atcacgcgct ggaactcctt gaccattttc gggaacgggt
    40321 gcggcccggc gaccgtcccg atcaggtagt gggtgttgtc cacgctggac acccagtcgc
    40381 gcatggcctc attcatcgcg tccttcagcg tctggctgcc ggactcgacc gcgatcactt
    40441 ctgcgccgag caactgcatc cgggccacgt tcagcgcctg ccggtcggta tcgacccggc
    40501 ccatgtagac ccggcactcc atgccgagaa gagccgccgc ggtcgccgtg gcgacgccgt
    40561 gctggccggc tccggtctcg gcgatcaccc tggtcttacc catccggcgg gtgagcagcg
    40621 cctggccgag aacattgttg atcttgtgcg aaccggtgtg gttcaggtcc tcgcgcttca
    40681 gcaggacgcg ggcgccgccg cagtgccggc tgaagcgcgg cacctcggtc agcggggtgg
    40741 gccggccgga atagttgcgg cgcaggttgt cgagttcgcg ccagaacccc gcgtccccca
    40801 ttgccttgtc gaactcggcc tcgagttggt tcagggcggc gacgagcgct tcgggcacgt
    40861 accggccccc gaagatgtcg tagtggccac cggcgtcggg aagcgttggg tttagcaccc
    40921 agctattcac tcacaaagtg cgggcatagt catgccagtg accgcagcgc ggccaccgca
    40981 ccccgcgggt cgccgtgccg cacgagcatt tctcccacca gcaccgcgtc cgccccggcc
    41041 gcgcgcatgc gggctgcgtc cgccgtgctg aggatgccgg attcggccac cttcaccgca
    41101 tcgtcgccga tcaggccggc catctcctcg aagtggacga gatcaacctc gagggtcttc
    41161 agattgcggt tgttcacgcc gatcagggac gcgcccgcgg ccaacgcccg ccgcacttcg
    41221 tccggggtgt gcgtctcgac caggcaggtc gtgccgagtt gtcggccgag ttcgaggaag
    41281 cgctccagtt gagtctggct gagcgcggca acgatcagca gcgcgaggtc ggcgcccgca
    41341 gcccggccct cccagagctg gtactccgtg accacgaagt ccttgcgcag cagcggcgtg
    41401 gccacggcgg cgcggacggc agccaggtcg gccagagagc cgccgaagcg ccgctgttcg
    41461 gtgagcacgg agatcgcgtt cgctccgccg gccgcgtact cgcaggcaag cgcggccgga
    41521 tcctgaatct cggccagtgc gcccttactc gggctcgaac gcttgacttc ggcgatcacg
    41581 ccgagacggg ggccgcgcag cgcgctcagc acctctaggg cgggcggttg atcgacacag
    41641 gccgcgatca cgtcgtccag gctgcgggca gcctgacgtt gctcgagatc ctcgaccacc
    41701 ccggcgatga tcccgtccag aaccgtcatg tcaggactgt ccgaacccca tccggcgcag
    41761 caccgcgcca gtgacgatcg ccagcaccag cagcacaccg gccgcccaca acagcgtccc
    41821 attcggtccg gtcaagaacg ccaccgcccc aaggatgaaa gccacggttg cgatgatgct
    41881 gccggcccag gccgcggggg tacggccgtg gtggtactcc ttggcgacct tcgcagtggc
    41941 ccgctcagcg ctttcgatca ttcgatcgct ccttcgagtc aatgtggaac cgccggtatc
    42001 ggaaagtcca tacaggtgat catcttgact gttatgcgct cacattgcca agtccccgat
    42061 cgaaggattg cctgagcaaa tctcaggaac ggacggattg ccgttcggct gagcgtgcag
    42121 ccgtcgtcag gacgctcagc tttcgtcggc aatccgacgc aaccgggacg cctccgcgac
    42181 cgcaccgagc actgctgcgg ccttgttcac gctctcggcg tcctcgttcg ccgggacgga
    42241 atcggccacc accccggcgc cggcctggac gtaggccacg ccgtcacgca gcaccgcggt
    42301 gcggatcgcg attgcggtgt cggagtcacc ggcgaaatcg aagtagccca ccactccccc
    42361 gtacaggccg cgccgcgaca cctccaggga gtcgatgatc tccatcgccc gcaccttcgg
    42421 cgcaccggag agggtgcccg cggggaaaca gctcagggtg gcgtccagag cggtccggcc
    42481 ggcggccagc gtgcccgaga ccgcggcctc caggtgcatc acgtggctgt agcggcgcac
    42541 cttcatgaac tcgaccacgg ccacggtgcc cggcacgcag acccggccga ggtcgttgcg
    42601 gccgaggtcg accagcatca ggtgctcggc cgcctccttc ggatcggcca gcagctcggc
    42661 ttccagcgcg gcgtccgcat cggtcgtggc gccgcgcgga cgcgtcccgg cgatcgggtg
    42721 ggtggtcgcc cggccggcat tcaccttgac cagggcctcc gggctggagc cgacgatcgc
    42781 gaacccgccc agccgcagca ggtagaggta ggggctcggg ttccgccggc gcaggacgcg
    42841 atagacgtcc agggcatcgg ccggacagtc ggtctcgaag cgctggctgg gcacgatctg
    42901 gaaggcctcg cccgcgcgga tctcctcgat cgcggccgac accgcggcct ggtactccgc
    42961 cgacgtgcgc tggcgacgca cctgcggcgt caccggggac tcgtcccgga cggcgaccag
    43021 cggacgggag ggctgctgga gcgcggcggc catcgcctgc acacggcgca ccgcgtcggc
    43081 gtaggcgtct tcgatgtgcg cggccgaatc gtcgaagttg atcgcgttgg ccaccagcca
    43141 cacttcgccg gtgtggtggt cgaggacggc catgtcggcg accagcatca tcgccagttc
    43201 ggcgatgccg aggtcgtccg ggttaccatc cggcagcttc tccaggcggc gcaccacgtc
    43261 gtagccgagg tagccgacca tgccggaggt cagcggcggc aggccctcgg tgcgcggcgt
    43321 gtgcagcagt tcgagggtgt cggcgagcac ttgtagcggg tcgccgccgg tgggcagtcc
    43381 ggtgatcgga cggtcggccc aggtccactc tgcgagcccg tcccgctcgg tgagcgtggc
    43441 ggccgcattc accccgacga acgagtagcg cgaccacacc ccaccttcgg cggattcgag
    43501 caggaaagtg ttcggccgct cgccacagag ctggtggtag aggccgacgg gggtgaagtc
    43561 gtccgccagc aggcgggtat ggacgctgac cacccgctgg gtggcgccgg cggcaaggaa
    43621 cccggcgagg tcaggggtga cggtgacgct cacttcgggg cccccacgga accgttcggt
    43681 agagcgcagc ggaggaacag gacttcgtgg gatggtctca tgcgccctcc gccagcagca
    43741 cccggtcgtc gaaacaggta accgagccgg tgtgacaggc cggaccgctc tggtccacgc
    43801 gcaccagcag ggtgtcgccg tcgcagtcca gccgcacctc gcgcacggcc tgggtgtttc
    43861 cgctcgtctc gcccttgacc cagtagctct gccggctgcg cgaccagtag gtgccgcgct
    43921 tggtggtcaa ggtgcggcgc aacgcctcgt cgtccatcca ggccagcatc aggacccgtc
    43981 cgtcggtcgc gtcctgcacg atcgcaggca cgagcccgtc cgggttccgc ttcagcaagg
    44041 cggccacgtc ggggttcagc tcagtcaccc gcacattgtg acaggttcgt ctcgtctggc
    44101 ccgaatgggg aggccggcct gccgaggtgg ccggacgggc cttagcggga ggtcctgcgc
    44161 gagcgatcat gcctctgcga gaagcaagtg ttcgaaccgc cgttggattc ggctcaacat
    44221 cttgcgggcg ggttcgcaat tcgccacact cgggccacca ccctcaacct gaggggagac
    44281 cgcctagcga aaagcccaca atgcgctcct tcatctcaac ctcaatggcg cagcgattcc
    44341 agctcagacc tgccttcggt cgccagccga ttcggatgcc atcccggata ctcaccctgg
    44401 cggcggccct cgtcctcggg gttagctact ttgtggtcga cacgacggcc cacgccgcgc
    44461 ccgccaccac tgacatcgac agcacccaac tcgtcgcccg tgacgcaacc aagacctttg
    44521 cttccggagt cgtccgcaac gcgaagggcg ccgccgccaa gtccggtacc gtaaccgcct
    44581 acgcgttccc aggtgctgcc gtcacgaaga acttgaaggt tggcgactcc ttcactctca
    44641 ccgaggtcgg ccgggctgag atccagttcg acggcagcta tgcactgagc ctcgactacg
    44701 acgcggtcgt acagaagagt ggcaccggcg gccggcccgt gaacatcgac atcgtggcct
    44761 ctaccaccga cgggaccgcg gtgtacagcg acaccgcgct ggtagaccct ggcaagaaga
    44821 agctcacggt caacgcagcc gccacaccaa ccacgaaggc ggccgcgagc acggcgggag
    44881 ttgctacgcg cagtgtggca gggacgagcc aggggctcgc tctggatctg gcagtcacgt
    44941 cgcagagccc agcgaaattg caggaaccgg ccagcatcgc cgtcgcgccg atggccagca
    45001 tccccagcaa ctgcaagatg gtgaaggatc tcggcaacgt atggacggtt gttggccaga
    45061 cagcagccac caccaccaac gcgcagcagc ggatttacta ccaggaggac gccacaacga
    45121 cctttggtgt tgcccttaaa gtgaacgggg tctacacgat gtcaggaacc acaaccttcc
    45181 aagcatcgac gaccgtcaac ttcccgatct acacagcgat tggggcgagg aagcacagga
    45241 cgcagttcaa gtacgccgaa ctgagttgca cctacggctc cacgcagatt gcccagatct
    45301 cagcgcgacc gattgcgttc gccaccggaa cggacgagtt gtggaccacg actccatcca
    45361 cccctgcgag caattgtgcg ccctacctgg ctggaagcgg gctcaacaag aagaacacca
    45421 cagcgtcgac attctcgaac ggcgtctcca tcaagtccaa gatcggcatt gacctgtcgt
    45481 cacgctccgg ctactcggcg accgtccggc actgggtgaa cttcaccaag cccgggaagc
    45541 tatgtggcgt ggatggagtc ccgggtgacg cgccaggact gatggttgtg aagtgagaag
    45601 gctcgccgtc gctggtctgg ccggcgctgc tactgtcgcc gtggtgagct gggccgggtg
    45661 ttcggtccag ccccaactca tctcggccac cgaaggcaca acactgtgcg tgcctgtgaa
    45721 cggcgacggc gaggcctact tgggacacga agtgttctcc aaccgcactg gtagcgcggt
    45781 ctccgccgca gcattcgtgc ctgaccaggt tgccggagcc tcggtggtcg gcggctactt
    45841 cgtgcctgtc ggcgaacccc agcccggcgt tggaaacgat ccggcactca tcccgcacgg
    45901 cttcactttc gaaccggtgc cacctggaga gcgcgtcatc ctggtcctcg gggtgaaact
    45961 ggacgacccc aacctcgccg gacagttcac cggcggcacc ctcacctcca cctcgggcgg
    46021 cgagctgggt cgctcctcga ccgggtatca catggtgccg gccggagagg tctgcgacat
    46081 gggtgaggag tgagcaccgg tttaggggtt caagtccgcg tccgaccagc aggtcggtgg
    46141 gtagggtgcg aaccatgagc ctcagtccgc aacgcgccgc cctctgcgat gccctcgata
    46201 ccgtcgaccc gtccgccccg accctgtgcg agggctggac ggcacatcat ctggccgccc
    46261 acgtgtggtt gcgcgagaac gaactgctcg caccagccgg ggccgtgttc aagccgctgg
    46321 ccggacgcct ggagacgcgg atggccgaag tcatgaccca acggtcatat gccgacctgg
    46381 tcgcgacgat ccgccgcggc ccgcaggggt tctcgctctt ccgcctgccg ggggcggaga
    46441 aggcggccaa cgagatcgag ttcttcgtgc actgcgagga cgtccgtcgc ggcgacggcg
    46501 accgcacgcc gcgtccgctc gatccggagt tcgaagacta cgcctgggga cgggtgcgga
    46561 cgatggccaa actgatgctg cgccgggtgc cggtggcgac gtggttgaag cgccacgtgg
    46621 ccgacgagcc gctgcgcgtg ggcaagggag ccgacatcgt caccctggtg ggcccggcca
    46681 gcgagttgct gctctacctg ttcggacgcc ggcccgcggc caacgtgacc ctggtcggcc
    46741 aggccactac catcgcggtg ctgcagcggg ccaagctcgg ggtctaggac ccgcacccca
    46801 ccgggatctg ggtccacgcc gagcgtcgcg cggcgatgaa ctgagacggc ctgccacaat
    46861 gtgggccatg cgcgtctaca acttctccgc aggtccggcc atgctcccgc ttccggtact
    46921 ggaagccgcc cagtccgaac tcaccgactg gcagggttcg gggatgtcgg tgatggaagt
    46981 ctcgcaccgc ggcaaggact tcgtggcctg cgctgcccag accgaggcgt tgctccgcga
    47041 agtgatgggc gtcccggaca actaccaggt gctgttcctg caaggcgggg cgtccggtca
    47101 gttcgatgcc attccgctga acctcaccgc cgctggcgac accgtcgact tcctgcacac
    47161 cgggcagtgg tcggccaagg cgatcaaggc ggccaggaag gccggcttga acgtcgaggt
    47221 gctcgccgac gaggccgcgt ccaaatacac caccacgccg gcggccggca gctacaccgt
    47281 caatccggca gcgaaatacc tgcactacac gccgaacgag accatcggcg gggtcgagtt
    47341 cggctacctg cccgaggcgg gcgacgtccc gctggtggcc gacctgagct ccaccatcct
    47401 gtcccggccg atcgacgtca cgtcctatgg cctgatctac gccggcgcgc aaaagaacat
    47461 gggaccgtcc gggctgtgcg tgctgctggt gcgcgacgac ctggtcggcc acgcgcgccc
    47521 cgaggtgccg tcgatctggg actaccaggc gatggccgag tcggactcga tgctgaacac
    47581 cccgc
//
LOCUS       DAOPRQ010000005        47037 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_564_length_47037_cov_11.417947, whole genome shotgun sequence.
ACCESSION   DAOPRQ010000005 DAOPRQ010000000
VERSION     DAOPRQ010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 47037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 47037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47037
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_564_length_47037_cov_11.417947"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1796)
                     /gene="rho"
                     /locus_tag="PKJ61_01045"
     CDS             complement(<1..1796)
                     /gene="rho"
                     /locus_tag="PKJ61_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013160937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOC12452.1"
                     /translation="MLLPELKQLGASLGLKGTGTMRKGALIEAIQTAQGSAKPERPAS
                     ERPKGGRARAKAAPSGEAADAGQPTLPRSAEADATVGEASAERTAEAAPARRSRARGN
                     QAERTQTTDREPAARADSGTADAGSAGVDDVAARLDALKAEESIRPRRNRGDGADGAE
                     ARNSETARQPQQNSQSQQNQQRNDRNDRDDDDYGSGRRSRRRRNRDRQNNNRRGGRAV
                     GGPAGLDRMEAEPVLAEDDVVIEISGILDVLDNYAFVRTTGYLPGPNDAYVSLSMVKK
                     WGLRKGDIVTGAIRAPKEGERREKFNPLVRIDTINAAAPEKAKDRPDFQKLTPLYPQE
                     RLRLETTGINMTGRIIDLVSPVGKGQRGLIVSPPKAGKTLVMQAIANAITVNNPEVHL
                     MIVLVDERPEEVTDFQRTTSGEVIASTFDRPADDHTTIAELAIERAKRLVELGRDVVV
                     LLDGITRLGRAYNLAAPASGRILSGGVDSAALYPPKKFFGAARNIEDGGSLTILATAL
                     IETGSKMDEVIFEEFKGTGNMELRLRRDLADKRIFPAIDPVASGTRREELLLARDELA
                     IIWKLRRVLSAQDPQAGLEMLLNRMRKTQSNR"
     gene            complement(2134..2730)
                     /gene="thrH"
                     /locus_tag="PKJ61_01050"
     CDS             complement(2134..2730)
                     /gene="thrH"
                     /locus_tag="PKJ61_01050"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016669768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoserine
                     phosphatase/homoserine phosphotransferase ThrH"
                     /protein_id="HOC12453.1"
                     /translation="MDLACLDLEGVLVPEIWINVAETTGIDELRLTTRDIADYDELMG
                     HRLRVLDAHGIGLTQIQAVIAEMGPMPGAAEFLDWLRERFQVLILSDTFYEFAMPLMA
                     QLGFPTLLCHRLQVQDDRVIGYTLRQAESKRTAVRALHELNFRVVATGDSYNDTAMLA
                     EADAGILFRPPANVVAEFPQFPVVTDYAGLRNAFSSAL"
     gene            complement(2730..4022)
                     /locus_tag="PKJ61_01055"
     CDS             complement(2730..4022)
                     /locus_tag="PKJ61_01055"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016669767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HOC12454.1"
                     /translation="MMTDSTPLKVALLGCGVVGSQVARLLIEQAEDLRARIGRPLELV
                     GIAVRRLEVERPGIDRALLTDDALALASRTDVDLVIELLGGIEPARSLILAAIGAGAS
                     VITANKALLAEDGGTIYAAAEQAGVDVYYEAAVAGAIPIIRPLRESLVGDEVTAVVGI
                     VNGTTNFILDKMHTDSADFGAVLAEAQELGFAEADPTADVEGYDAAAKAAILASLAFH
                     TRVNLADVDREGITGVTGEDIAAAKEMGCVIKLLASATLSDDDTIAVGVHPTMVPVAH
                     PLAAIPGAYNAVFIESRHAGRLMFMGPGAGGSPTASAVLGDLVTAARNRVRGARGPAE
                     STYSGRAITPPGKVASRYYVSLTVHDEPGVLAKVAATFAKHGVSLQAVRQESTGAQAR
                     LGVMSHLALGEQTDAAIAELGGMPEVHPGIAVMRVQGA"
     gene            complement(4019..5437)
                     /gene="lysA"
                     /locus_tag="PKJ61_01060"
     CDS             complement(4019..5437)
                     /gene="lysA"
                     /locus_tag="PKJ61_01060"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HOC12455.1"
                     /translation="MTHMHVAGSIHADVTTPAPVWLTRPEDVNALSDKLWSKTVKRDD
                     EGVIQVAGIPVTELAEQVGTPAYVIDEDDFRERAREWRNAFEGWTVFYAGKSFLSGVI
                     ARWVGEEGLNLDVCSLGELTVALRAGFDPARIGMHGNNKSEAELRLALVRGVGRIIVD
                     SSDEITRLESLCAELDTTANVMIRVTTGVEAHTHEYIATAHEDQKFGFSITGGQALVG
                     MFRCHHSPRLNLLGVHSHIGSQIFDTNGFEVAARRTLKLFAQFKEATGIELSELDLGG
                     GFGIAYTKEDTPASPSELASGLREIVAHECAGFRLAVPAISIEPGRAIVGPSGMALYR
                     VGTVKPVDVGGGLSRRYLSVDGGMSDNIRPALYAAEYSAALANRTSAAAPVVSRVVGV
                     HCEGGDILVRDEFLPADVAVGDLLAVPAAGAYSRSMASNYNHTPRPPVVSVKDGTVSV
                     MVRRETLDDLLALDAGLADEEA"
     gene            complement(5466..5981)
                     /locus_tag="PKJ61_01065"
     CDS             complement(5466..5981)
                     /locus_tag="PKJ61_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC12456.1"
                     /translation="MSGARRLLAAALLLVLLSGCVAKAAPAPAELRQTPPPWPAPRDG
                     IAYFEAAGVPASRLDDRSNQRVVTVAISIDDVGVPLVPNIGVDRPRALQAPVHTHDAS
                     GTVWLEGQGVQDVTLGQFFTIWGVRFDERCLGAACGGVQVSADGAAVATPAELRLAEV
                     SDVQIRAASGS"
     gene            6052..6126
                     /locus_tag="PKJ61_01070"
     tRNA            6052..6126
                     /locus_tag="PKJ61_01070"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6085..6087,aa:Arg,seq:ccg)
     gene            6370..10206
                     /locus_tag="PKJ61_01075"
     CDS             6370..10206
                     /locus_tag="PKJ61_01075"
                     /EC_number="4.1.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional oxoglutarate
                     decarboxylase/oxoglutarate dehydrogenase thiamine
                     pyrophosphate-binding subunit/dihydrolipoyllysine-residue
                     succinyltransferase subunit"
                     /protein_id="HOC12457.1"
                     /translation="MATPGSGVDTNDSLAEFGANDWLIEEMREQYLADPESVDASWVE
                     YFRSHQGASADGTQPAAAPSAAPTAPAAATQAPEVQAPATSAAAETVQVVQVPIVDVA
                     EPEPESRPLAEAPVNEPAVEAVQPNAENAPQITGAASGVSANRPKQAPQPTKDATEPV
                     RTPLRGAPARTAVNMDASLSMPTATSVRQVPMKLVIDQRQMINAHLARSTGGKVSFTH
                     LIGWAMVKALLAVPEMNNSYELVDGKPNLVTPPSINLGLAIDFKKADGTRQLLVPNIK
                     NCEDLDFAGFWAAYEELVGKARRGELTVADFAGTTATLTNPGGIGTNHSVPRLMAGQG
                     LILGVGSIDYPPEFQGASQARINQLGVSKVTTLTSTYDHRVIQGAQSGEFLKVLHELL
                     IGQHGFYDEIVHSLRVPYTPIRWANDVNDERVGSIPRSARIFSLINAYRNLGHYMADI
                     DPLEYRQRSHPELDLEYHGLTLWDLDREFPVGSFGGRSGEVHTLRDLLVMLRDSYCRT
                     VGVEYMHIADNEQRTWIQDRFERPFVPWPREEHLRILDKLNEAEIFETFLQTKFVGQK
                     RFSLEGGESAIVFLDEVCEQAADAGLSEAIIGMPHRGRLNVLANIVGKSYGQIFREFQ
                     GNYDPRLSQGSGDVKYHLGSEGEFKAASGRTIKTSVAANPSHLEAVDPVVQGIARAKI
                     DQIGKIEGWPVLPILMHGDAAFAGQGVVYETLQMSQLRAFKTGGTVHVVVNNQVGFTT
                     APIESRSSVYCTDVAKTVQSPIFHVNGDDPEAVARIAQVAFDFRQRFAKDVVVDIVCY
                     RRRGHNEGDDPSFTQPLMYDLIEQKRSVRKLYTEALIGRGDISTDDADEVIAKFRDRL
                     ESVFKEVREEPQDEPISKTPYYPAKLGKDVGTAITAEAMEAVAKAHLTFPEGFTVHPK
                     VLPQLERRADAIRKGPIDWATAELLAFGSLLMEGRPVRLVGQDSRRGTFSQRFAAVID
                     RVDNRAYVPLKHLSPDQGRFHVFDSLLSEYAGMGFEYGYSVAAPDALVLWEGQFGDFA
                     NGAQTIADEFISSGNAKWTQKSGVVLLLPHGYEGQGPDHSSARIERWLTLCSEGALAV
                     CQPSTPASYFHLLRTHAYVNWHRPVVVATPKSMLRSKASMSPVEEFTQGRWQPAQGDP
                     TITDPSAVRRILLCSGKIRWELTAARAEAGLDGQIAILPLERLYPLPTKELAAELAKY
                     PQVTDIRWVQDEPENQGAWWFLQLYLADAVRTILPDYELRMTGITRAAASAPSVGSVK
                     VHQAQEADLLARAMAD"
     gene            10206..10382
                     /locus_tag="PKJ61_01080"
     CDS             10206..10382
                     /locus_tag="PKJ61_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020389457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6104 family protein"
                     /protein_id="HOC12458.1"
                     /translation="MYFTDRGIEELAARRGDEEVTLAWLAEQLSTFVDLWPEHENAVE
                     RLATWLARLDDEED"
     gene            10387..11520
                     /gene="galK"
                     /locus_tag="PKJ61_01085"
     CDS             10387..11520
                     /gene="galK"
                     /locus_tag="PKJ61_01085"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012918960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HOC12459.1"
                     /translation="MTQAAVEAFEELTGSAPAGVWAAPGRINLIGEHTDYNDGFVLPF
                     ALPQAAYVAASRREDGRWRFRSVDLDDTVEIDADGLVPGTDGWHSYLAGVIWALRQAG
                     HEVGGADLVLTSDVPLGAGLSSSAAIECAALAALADLYDLTIEPMDRAKLARVAENAY
                     VGAPTGLMDQAASTLCVADHALFLDCRSFDTRQVPLAVTQAGLEILVIDTRTRHSHVS
                     GEYADRRASCEEACRLLGVAALRDVTDLDAALAVLPEEIGRRVRHVVTEDRRVQDSVA
                     ALDADNHAQLGALMTASHDSMRDDYEITEPSVDLAVATALASGALGARMTGGGFGGCV
                     LALVAAEQAEAVGAAVTSAFAGAGHAEPVTFLARPSAGARRVR"
     gene            complement(11474..11878)
                     /locus_tag="PKJ61_01090"
     CDS             complement(11474..11878)
                     /locus_tag="PKJ61_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC12460.1"
                     /translation="MSRATLERPAGLLVAVAGSGLVGLAILALAIASLAAGHGEFSGA
                     VGIALVVYGLLILAATWALWRGSLFGRGPVIATALLNLIAAASFTPGAPWAWLVVLVA
                     AATVVAAALPSTSRALHLSGRAGLQRTDEPGK"
     gene            12084..12335
                     /locus_tag="PKJ61_01095"
     CDS             12084..12335
                     /locus_tag="PKJ61_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HOC12461.1"
                     /translation="MDWRHQAACLTEDPELFFPVGNTGPALMQIAEAKKVCRRCDVRE
                     ACLQWALDAGQDHGVWGGLSEDERRAMKRRAARMRVRNG"
     gene            complement(12377..13828)
                     /locus_tag="PKJ61_01100"
     CDS             complement(12377..13828)
                     /locus_tag="PKJ61_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002516828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOC12462.1"
                     /translation="MQTMEQVIARHCVLEDEDREWLELLVDEWSLLADLAFADLILWV
                     PDADDNVFWACAQVRPTTGPTSLEDDVVGEEIAYDPEHLVTEAYLSREISHTSGNKLH
                     AGIPVDVQAIPILADDRCLGVVEMHTNQMGVRAPGALEDAYLEAAQVLAGMVHRHQFP
                     LGGDRRVPWVSPRVGDGMIRVDQAGMIRFASPNALSAYRRLGLTSDLYGENFVAVTRS
                     LLREKVPVERPVAASLAGRGSAELDLETTTANVRIRILPLTGEDGPDGLLVMCRDTTE
                     LRSRERQLVTKDATIREIHHRVKNNLQTVAALLRLQARRIESPEAKGALSDAMKRVAA
                     IAVVHEILSQAFDSSVKFDDVADRLLRMVGDVAATGGKVRMVLQGSFGYVPADVATSL
                     SLVLTELCQNAIEHGLRDKAGEVLVIPQQSDGELNVVVKNFGEPLPEDFRIGRSTSLG
                     LSIVSTLVADLGGQFTLASDDDATTATVRVPLR"
     gene            complement(14256..14513)
                     /gene="rsrA"
                     /locus_tag="PKJ61_01105"
     CDS             complement(14256..14513)
                     /gene="rsrA"
                     /locus_tag="PKJ61_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012922784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycothiol system anti-sigma-R factor"
                     /protein_id="HOC12463.1"
                     /translation="MSEFEDCTEVLRNLYAFHDHELTPAEADEIRHHLLACEGCLDHY
                     DVEAALRMLVRRSCCQSAPESLRLRVRAIYSQTILITELTD"
     gene            complement(14506..15126)
                     /locus_tag="PKJ61_01110"
     CDS             complement(14506..15126)
                     /locus_tag="PKJ61_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016669760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOC12464.1"
                     /translation="MNLPTKPSETDTGLDESKAERDHRFETEALAYLDQLYGAALRMT
                     RSPSDAEDLVQETFAKAYASFHQFTPGTNLKAWLYRILTNTFINTYRKKQRQPQHSGQ
                     EVEDWQLARAEAHTSTGLRSAEMEALDRMPHSAVTDAMNALAPDFRLAVYLADVEGFA
                     YKEIAEIMGTPIGTVMSRLNRGRNQLRANLADFAREHGLIGDSDGE"
     gene            complement(15253..15933)
                     /locus_tag="PKJ61_01115"
     CDS             complement(15253..15933)
                     /locus_tag="PKJ61_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HOC12465.1"
                     /translation="MCGRYAVSADVDELTEIFEIDEVAGPLPGPDYNVAPTDPVPAIM
                     ERDRADGRRRVLAEVRWGLVPSWSKDATGAARLINARVETLAEKPAFRRAFAARRCLL
                     PADGYYEWYQLPDSKTKQPYFIRPAGGGLLVMAGLYEFWKAPDGTWLATTTVITTAAA
                     DEVGHIHDRMPMVVAPENWEAWLDPGFGMDARALLTVPAPSLTYYPVSTAVNKVANDG
                     PELITPLS"
     gene            16018..16605
                     /locus_tag="PKJ61_01120"
     CDS             16018..16605
                     /locus_tag="PKJ61_01120"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HOC12466.1"
                     /translation="MSVLRFVARSMLASYFVLNGVRALAHPEDLAPATAPIAEKLMPK
                     LSAALPESARAFVPTDATGLVKLNGLLQIVGGASLATGFFRRIGAGVLAVTTVPNLLA
                     ANPLTKGPGQAAAQAEFGTNVALLGGVLLAAQDTEGKPTLVWRLRTQRELLAKDAAKR
                     KAELERDARATAADAAKLARKSVKQGRKFVAEVLS"
     gene            16602..17876
                     /gene="aroA"
                     /locus_tag="PKJ61_01125"
     CDS             16602..17876
                     /gene="aroA"
                     /locus_tag="PKJ61_01125"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020575823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HOC12467.1"
                     /translation="MSAWTAPVATAGVRGHVAVPGSKSASARSLLLAALADGPSVLSG
                     VLDSRDTTLMRVGLSTLGAAFHDLPDGRLEVRPAVAVQGGGEIDCGLAGTVMRFLPPV
                     AALASRPTRFFGDPHASARPMTPLLGALASLGAAVSTPYQLPFTVASGAFHGGSVTVD
                     ASGSSQFISALLLAAARFIDGIEVRHTGATLPSLPHIEMTCQLLERRGVAVTRPGMNT
                     WRVASGPIAALDEAVEPDLTNAATLLAAALVTGGELTTSWPDGVQATSALADALAAFG
                     GQLSYNGAGADRAITLSGAGGLRGADVDLAAISEFTPAAAALAALAPGPSRIRGVAHI
                     RGHETDRLAALSTELGRLGAAVRQTADGLEFAPATLHGGSFATHADHRMAHAGALIGL
                     VTPGVELDDVACTTKTLPDFPGLWARLIGREA"
     gene            17873..18865
                     /gene="rsgA"
                     /locus_tag="PKJ61_01130"
     CDS             17873..18865
                     /gene="rsgA"
                     /locus_tag="PKJ61_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HOC12468.1"
                     /translation="MSRRSREAITDDPHAGFDRPGKRTRPRTKDRPTYTAAVEAMVVT
                     VDRGRFRCLLDDDTIVTTTKARSLGRTGVIVGDRVRLVGDTTGDEGTLARIVEVQERE
                     TVLRRTADDDDPFERPIVANADQLVIVAALADPPPRVGMIDRILVAAYDAGLEPLLVL
                     TKADLADPEGLMATYRPLGVSVVAVEPGADLSTLREALDGHRSVLVGHSGVGKSTLVN
                     ALIPGVDRQVGEVNEVTGRGRHTSTSAVALPLPEGAGWVIDTPGVRSFGLSHVTPDSI
                     ISAFPDLADIVTDCPRGCRHLSSDPECALDVAMSEGRLAAERLESFRRMEKAFG"
     gene            18933..19721
                     /locus_tag="PKJ61_01135"
     CDS             18933..19721
                     /locus_tag="PKJ61_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020389511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HOC12469.1"
                     /translation="MPDQSLLDDLRLAHVLADQADSITMARFKASDLKVTAKPDLTEV
                     TDADTAVEDAIRAMLGRTRPRDAVHGEERDDSGWGPRRWVIDPIDGTSNFVRGVPVWA
                     TLIGLMVGDEVVVGVVSAPALSRRWWAAKGGGAFTGRSLLQGTPCRTSAVASMEDASL
                     SYSSIHGWVETGRGQQFVDLMRDCWRTRAYGDFWSYMLVAEGAVDVAAEPDLALHDMA
                     ALVPIVTEAGGRFTNLDGSAGPHGPGAIATNGPLHDEMVERLAL"
     gene            complement(19819..20970)
                     /locus_tag="PKJ61_01140"
     CDS             complement(19819..20970)
                     /locus_tag="PKJ61_01140"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR00045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HOC12470.1"
                     /translation="MSLRIVCVPDSFKESMTAVQAAAAMARGIRRAVPDADCVEVPMA
                     DGGEGTCRTLVAALQGVSVPVPCHDALGRPIVGEFGWVADRRLAIVEVAAGAGLELVP
                     THLRDVRTASSFGAGELLRAALDAGASELLVGLGGTATNDAGAGLFSALGVRFLDSAG
                     EPLPSGGEALARLASVDCAGLDPRLAAVRIELASDVTNPLLGPSGASVVYGPQKGADA
                     AAVTELDAALTCWADVVESSLGVSVRDRAGAGAAGGLGAAFLAFTGAELVPGVELVSR
                     AIGLAEHIVGADYVFTGEGSVDAQSLAGKVPHGLAALAAAVGVPTVVFAGRVEPGLSS
                     ADVLAYVPISGTVADLPAALAAGEWNLELAAERVTRELVSGRARWTETL"
     gene            complement(20973..21758)
                     /locus_tag="PKJ61_01145"
     CDS             complement(20973..21758)
                     /locus_tag="PKJ61_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016669753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOC12471.1"
                     /translation="MTEPVFRAEGLTRRYRNGGAEVVALDSVDLTLDRGRRLGIVGES
                     GSGKSTLVRLLAGLDRPTAGRIWFEGTEITGLPERRLGFLRAAVQMVFQDPRSSLNPR
                     MKVGDIITEPLRSRLVRKELADGDHRARLAEVLAEVDLPADSADRYPHEFSGGQRQRI
                     AIARALAPRPDVLIADEPVSALDVSVRAQVLNLLTDLVRSRGLTLIVVSHDLLVVRHL
                     CDQLAVMRGGRIVEAGATGAVYSQPRQEYTRALLAAIPRIKVG"
     gene            complement(21755..22585)
                     /locus_tag="PKJ61_01150"
     CDS             complement(21755..22585)
                     /locus_tag="PKJ61_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002513433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOC12472.1"
                     /translation="MTLLDVRDLSVSFGRSRTPAVRGISFDLAAGERLGVIGESGSGK
                     TVTALAVMGLLPENATVTGSISLNATEIVGLDEVGMARLRGDVISMVFQEPMTALDPT
                     MRVGRQVAEVLRLHQGAEPGAARERVVSMLGEVGLTDPQRVADAFPHQLSGGQRQRVI
                     MAMALINSPDLVICDEPTTALDVTVQARVLEKLDEVLTASAAACLFISHDLAVVSQVC
                     HNVLVMLDGEIVEQGTVADLFNRPQHPYTRGLVATARLDQIEPGERLPTVEDFHARRP
                     "
     gene            complement(22582..23394)
                     /locus_tag="PKJ61_01155"
     CDS             complement(22582..23394)
                     /locus_tag="PKJ61_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013160911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOC12473.1"
                     /translation="MKKAQLVAGLVLVALVVITAIVAVFWTPFDPIRVVPSQRLLGPG
                     WPHVLGTDGFGIDIASRLMIGARTVLVVGIISVGLASIVGVPLGMLAGQLGGWVDEVV
                     MRSADIVYAFPAILLATLLAAVYGSSTLTAMIAIGVATIPAFARVARAGAKQVMASDF
                     VLAARASGTSRWGVAGRHVLPNIAPLIGVQASVGFAMAILAEAALSYLGLATPRPNPT
                     WGNMLRDSQQFLVTAPLQALWPGLAIALAVLGFNLLGDGLRDYLDPRLREVS"
     gene            complement(23391..24368)
                     /locus_tag="PKJ61_01160"
     CDS             complement(23391..24368)
                     /locus_tag="PKJ61_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016667749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOC12474.1"
                     /translation="MHVAGRIGRRLGVFAISLLGASLLIFLITKALPGDVAQVLLGTD
                     ATPESVAELRRELGLDRPLIVQYLSWLGGILVGDFGHSHLSGQPVLALLAPRIAVTSW
                     LVGFGMIGSLIIALPAGMVAALKRRSWPGFAISAAAQVGMAIPVFWGGILLVLVFAVW
                     LRWLPANGYVSLTKDPGQWAAHLVLPVVTLAAVQGAILIRYVRSAFIEVLNEDYYRTA
                     RSVGWTPMRALLRHGVRNAAISLVTVLGLQLASVLVGAIVVESVFNLPGLGSMLLSAV
                     AQRDLLVVQGTVMFLVLAVLLISALVDFSYLLIDPRQAAVDSADGGAAS"
     gene            complement(24381..25955)
                     /locus_tag="PKJ61_01165"
     CDS             complement(24381..25955)
                     /locus_tag="PKJ61_01165"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOC12475.1"
                     /translation="MTYVAAESRRSRTGISKVLVAMLAMSMMVLSACTPTAVPSGTAT
                     TGDTSAPAPEAPRTLKIAATLEPASMDPWHNPAASIPQVLLYNVYETLVKVDSSGLLK
                     PLLAQAWEISDDRLTYTFHLNPAAKFASGAAVDAAAVVANINRLKADTKLVDALGLQL
                     AVVASAEALDATKVVIKLNKPSFMWLYDMSSTLGMILDPAETGDLATSTAGSGPYVFK
                     AQNRGQNVILAANTAYWANPARFDEVTFTYFTDPNAENAALSSGDQDVISNLQAPDAL
                     PQFADTSQFTTTSGTTNGEVVLGLNHESKALAKLEVRQALTMAIDRQALRDTVWNGQG
                     ALIGSMAVPTDPWYEDLTGTNPYDPEKAKALLKQAGYESGLTLRLRVPVIPYAVKSAQ
                     FVASQLRDIGVKATIEELEFSRWIDEVYTKADYDLTIVAHVEPRDLGKFADPKYYWRY
                     GDPEFASLYAAADSAASDEQAVAGFKQAVKYLADDCAAIWLFSLPNLVITKSNISGVS
                     TNAPGLSFDLTSITSR"
     gene            26127..26200
                     /locus_tag="PKJ61_01170"
     tRNA            26127..26200
                     /locus_tag="PKJ61_01170"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26161..26163,aa:Met,seq:cat)
     gene            26755..27612
                     /locus_tag="PKJ61_01175"
     CDS             26755..27612
                     /locus_tag="PKJ61_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013860961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ScyD/ScyE family protein"
                     /protein_id="HOC12476.1"
                     /translation="MLTTAVGAPFNLDVTRGKVLVADGGGPGVPITASGLTITGPDGF
                     KVTADTLAHENANNPDGKVFYGVRNPSQCVTDELNRIQLPVAHTGHIDSHAYSVAAYG
                     KGFVVADAGANALLWVSRSGKISTLAVLPAQPARITAEGAATLGLADCVVGVKYRFEA
                     VPTDVEVGRDGYLYVTTLPGGPEDPSLGARGKVYRVNPWNRRVREVASGLLGATNLAI
                     ADGKIYVTELFAGRVSLVKHGRVSEYVSLPGALSIEAGPRGTLYAGTGIAGPSSVVKI
                     STSGKGWRH"
     gene            27787..28431
                     /locus_tag="PKJ61_01180"
     CDS             27787..28431
                     /locus_tag="PKJ61_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HOC12477.1"
                     /translation="MAHRSGSELLALHGVRVLGGPSVAAVAARFRLAPTDVREHLLDA
                     QAYGWVNRHEHYGETWSLTASGKAEDERRLGAELDAVGCRDVVTAAHRAFLPLNRRHG
                     EACTRWQLRPQPWDALAANDHTDLRWDHAVLTDLDGIDAGLTHGLEPVATKLERFGGY
                     AQLHRQALVKVRGGHPEWLDSPAVESCQLIWIQLHEDLLATLGIPRGSDTVAPE"
     gene            28436..28633
                     /locus_tag="PKJ61_01185"
     CDS             28436..28633
                     /locus_tag="PKJ61_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC12478.1"
                     /translation="MKSLQQNPCQTKCDWYPTGEVVAEDLVFACTSCGSEWTRQEGWK
                     PRNLDGSVAAEVELELARDGS"
     gene            28695..29057
                     /locus_tag="PKJ61_01190"
     CDS             28695..29057
                     /locus_tag="PKJ61_01190"
                     /inference="COORDINATES: protein motif:HMM:NF016854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HOC12479.1"
                     /translation="MAYDQELAGRIRAALSFQTGIVEKSMFGGRAFLKDGHLLASASG
                     RGGMLLRVQPEVASALVAEGSASFFMMRGRELPGWLHVELDPADERLTAFLDLGLAFV
                     ATLESKAVNRLGPDRSRM"
     gene            29062..30393
                     /locus_tag="PKJ61_01195"
     CDS             29062..30393
                     /locus_tag="PKJ61_01195"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOC12480.1"
                     /translation="MEYVPESVIAYPIHTRSLPNGLRVVVSPDHSVPAVAVNLWYDVG
                     SRNERPGERGWAHLFEHLMFSGSEHVASGEHLNALQAVGGIVNATTWFDRTNYFETLP
                     VGALELALWMEADRLATLPDHLTEAAVDTQREVVKEEKRQRYDNVPYGDAMEHLVELA
                     FGPEHPYGHTTIGSMADLDGATVAGAASFFRTHYRPDNAVLTLVGDITPKEAFKKAER
                     AFGKVPGGAARTFGALNSSRPSSPANPSVPPLAGTPTRKLSGRVPAAATYFAWRLPPR
                     DTPAFDACDLALAALGAGQTSRLHQRLVREQQVAAGAGASALPLIAGNSLGLVQVRSL
                     PGDGGAAAAAAALAEVERLAETGPTQAELDRAKIQFEREWLTQLSRFDARADTISAFA
                     VLHGDPERINRRLDEVRSINLDDVARACATYLQPAQRAQLDYSQEADHAEG"
     gene            30380..31717
                     /locus_tag="PKJ61_01200"
     CDS             30380..31717
                     /locus_tag="PKJ61_01200"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOC12481.1"
                     /translation="MRKADQRPPVKAAPLWHFPEPAKLWLDNGFEVLANHRPGQYVAS
                     IAIVLDAPLSEEPAEIEGVATITQRCLDEGTRTHPGAGFAEALEDIGAILGGSAGYSA
                     SELSCDVPVSRLPQALRLLAEAVREPELRPDDIQRHQTLRLAEIENTLANSTQRAQLA
                     FRAACVPGRFRSARMAGGQAATVSQVTVDDVAAFHRRYYRPDGTTLIVSGDLTKAVFR
                     EAEAVFGDWDVPAPNTVRHQSPVSRRPHCWLIDRPGAVQADVRLGTFGIDRGDPRWAD
                     VQVATYALGGAFLSRLNRELREELGYTYGAHLVNTPMRDGGLISVQGSFRTEVVADAV
                     SRARDLLNVANRPFTSTEVADAVAYANGVSPLRYSTAQGVTDRIATLVADGVNPDFVN
                     TNALALTRVTPESATQAITELLPPDQLSLVVVGDAAQLAEPLRDAGWTMSVKP"
     gene            complement(31761..34661)
                     /locus_tag="PKJ61_01205"
     CDS             complement(31761..34661)
                     /locus_tag="PKJ61_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HOC12482.1"
                     /translation="MNVASQAVRRSPLLPTIAVVGVLVTGFIIFAELWTRKLWFDSVA
                     FPQVFTTQLLAQVLLFAIFFVVMALSVGLNMWIAFRLRPETRRTGQSAVLDRYRDLLE
                     ANIWVAILVPSVFLGLIAGTSVLSQSMEYLAWWNRTPFGTTDPYFGMDIGFYVFEYPI
                     LRDLLAFIMSALFFGLVAAAVVHFAVGGIVGGRDRGQSGRGNAARIHLSILGGLLLVG
                     YGLQTLLDRYGLLLQPDALFTGIQYTDAHSRFNAKLVLAIIAFVCAALFFANGFLKRW
                     TLPITALVLLLVSSLILSVAYPMIMQGFQVKPNEPDFERPYIQAHLEATRTAYDIADT
                     EVEEYEAVTQVSKGQLKEDAEALPGIRLIDPAVVPRTFENQQQLRGYYTFPEVLDVDR
                     YVIDGKETDAVVAAREINIAGLPDQAWNNLHTVYTHGYGMVAAYGNQRSAAGEPVWIE
                     KNLPPEGVLPDYEGRIYFGEKSTTFAIVGRLPGQAPIEFDSPGGGQGAGEVNNVYAGT
                     GGVPIGDLFTRVLYAAHFADWNFLLSDRVNAASKILYNRTPKERVAEVAPWLTLDSNV
                     YPAVVDGRLVWIVDAYTTTANYPNSQLTSLRAATTDSQSDAVGTQVEGQVNYLRNSVK
                     AVVDASNGTVDLYAWEAEDPILQSYMKAFPGTVKAKADISTDLMAHLRYPEDLFKVQR
                     QLLTRYHMTDPNSWYQQSDLWQVPADPTLTTPGQSAETARSGPAEPPYYLSIKWPGDA
                     SPVFSQTGVFVPNGRANLASYISVVAEASSPDYGRMRVLRMSDTHQIAGPSQTLTAMT
                     TDQKFAEVMRPYLNQGSAAAQYGNLLTLPMGSGLLYVMPIYTIRESGSGSGTYPALQY
                     VAVRFGEHVGIGSTLQAALDQVFSGDAGADTGENPVPENPNNPTEPTTPAEPTEVTPA
                     DQKAATEAMQAAETAFTAAEAALRNGDLAEYQKQVTAAKEAVATALEKLGQR"
     gene            complement(34703..35212)
                     /locus_tag="PKJ61_01210"
     CDS             complement(34703..35212)
                     /locus_tag="PKJ61_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016456178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPA1309 family protein"
                     /protein_id="HOC12483.1"
                     /translation="MSEALTAALIEVERHVGRSGWDQPARLFALVPTADLIAAEPSLA
                     EHLQGGFADGLSSIEQEEFHAGDDLAVTLSRIAWPTTVSGCALALERSFLPADAEADL
                     PDDPQAAAELVATHPKRIDLRVVVGVTRDGGRHSVARVRPSDDSEGELLGGADLVPAL
                     ASALAHTLD"
     gene            complement(35212..36264)
                     /locus_tag="PKJ61_01215"
     CDS             complement(35212..36264)
                     /locus_tag="PKJ61_01215"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HOC12484.1"
                     /translation="MTKQTWTAAVSAALFVVLAALIAIIPVPYVTWAPGSTADLLGRV
                     GEREAITISGARTFPTSGELRMTTVSVTAPGVTLSLPEVLFAYWLPAREVLPKDAVYQ
                     PGKDATEISQVESQRMVESQSDAVVAGLRQSGIEVTSWPRVAEVFTSGASVGILEPGD
                     LIKAVDGTATHTVQDVRDMILNDNRHPGDPVLFTVLRNDLMLKLTVTTRATTNEPDKP
                     IVGINMDVGYSYAPTVEFAVDPAVGGSSAGLMFALAISDRLVSADIAAGRVIAGTGTV
                     SSEGNVGAIGGVQEKIAAAVTDHATIFVLPRANCADAGTPDPSIRLLPVDTLAEATDS
                     LAKLADPAEADSVAGC"
     gene            36372..37709
                     /locus_tag="PKJ61_01220"
     CDS             36372..37709
                     /locus_tag="PKJ61_01220"
                     /inference="COORDINATES: protein motif:HMM:TIGR03624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HOC12485.1"
                     /translation="MADEGFDNLPERGGEEEALLRFLAQFGITPGPDGRLDLEGLMTQ
                     LQPLMSNLTAQLASFGKSDASGMNWGFTRDVVRKVVGAAGDDPEPTPAQLAAIRDAVA
                     LADLWLDEEIAFPRLSAPALAWRRAEWSEQTYSTWQRLVRPVVTQLSLALRGLMTGGT
                     GESKAAEPMLRMALSGMFAAQVGQSLGSLATTVISSGDIGLPLTETPQVALLPTNVAG
                     FAEGLEADPADVLLFLAVRETARQRLFGAVGWLGPQMLALVEHYARDIEIDPEALEQA
                     IESQLNAAMTAGELEQAGSALAGSLFAPRVTDEQRQVLDRLETLLALVEGWVDEVVAQ
                     VAGQRMPAAAALTETLRRRRAAGGPAESALKALVGLEMRPRRSRDAANLWAATRAARG
                     GESRDATWDHPDLVPTADDLADPLEFAAQGHRTAAPDDLDAELAKLLSEEGRG"
     gene            complement(37751..38308)
                     /locus_tag="PKJ61_01225"
     CDS             complement(37751..38308)
                     /locus_tag="PKJ61_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HOC12486.1"
                     /translation="MVAESELEVRRSARRTRTLTVFRERGRLVALVPARMTAAQEREL
                     LPPLVERFLVKESRRLAPVLADELSARAVDLFQRYLAERVSAPLPGFTVKWSTNQQRR
                     WGSCTSSTGAIRLSARLRTMPTWVSDYVLIHELAHLFEPNHSVAFRRLEAGYPKAAEA
                     KAFLAGYQHAQDGGDEDWLGADEVD"
     gene            38558..38719
                     /locus_tag="PKJ61_01230"
     CDS             38558..38719
                     /locus_tag="PKJ61_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013160897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5679 domain-containing protein"
                     /protein_id="HOC12487.1"
                     /translation="MAKDTYSGSFYCVKCKDHREATGDVVVNEKGTRIAKAKCPVCGT
                     NLTRFLPKA"
     gene            39039..39860
                     /locus_tag="PKJ61_01235"
     CDS             39039..39860
                     /locus_tag="PKJ61_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC12488.1"
                     /translation="MLRVRLRSPITSLWRAPGVLQIGLDAPAVVLDGVPAQLRDALRL
                     LTSPKSVAELAALLPKLDARWLGWLVARLDDAGLLAFESAAPPEPRVTVVGAGPLAEA
                     VRAALASPDVSVGGLDHAAYFGGSLEADGTPDDVLTVVAAANSEPDRTLTDALFRSGR
                     AHLVVRLESDRAVVGPLVVPGRTPCVRCLDLTRCHLDADWPRLLAQLCLEATEPEPSL
                     VTWAAATAMAQVRAWTAGRMPETGGGSLELALPNYLLTARRWPAHPRCGCLQPLG"
     gene            39883..41202
                     /locus_tag="PKJ61_01240"
     CDS             39883..41202
                     /locus_tag="PKJ61_01240"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007077138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HOC12489.1"
                     /translation="MTSQDEERRVLPESTLTRSAKLLTLPLGAAARAGVGMGRKLLGA
                     PAEDVDAAMRDAAAEQLFQVLGELKGGAMKFGQALSLFEAMLPEDLAGPFRERLRKLQ
                     DAAPPMPSARAQGVLRAELGPRWRDHFAELNLRPAAAASIGQVHRGRLADGREVAVKI
                     QYPGADTALASDLRQIQRLAGAVSPLTGGLDVVALVREVAARVTEEVDYTLEGVSQQQ
                     VATALAGHPRFLVPNVHLATRRVLVSDWVDGEKLTAIIDRPQADRNRVALDYVTFLFA
                     GPSLAGILHGDPHPGNFLVMPDGRLSVVDFGLVSRLPDGLPEAMGRLIRHAVDGAVEP
                     MVAGLAEEGFVDDSMDGRALLDYLAPFVEPASVEDFAFNREWARSQFARVHDDMGSSG
                     VATRLNIPPEYSLIYRVWMGGIAVLSQLDVHANFAQVLRDYLPGFAD"
     assembly_gap    41236..41335
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(41458..42333)
                     /locus_tag="PKJ61_01245"
     CDS             complement(41458..42333)
                     /locus_tag="PKJ61_01245"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012917841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOC12490.1"
                     /translation="MSDHYLSVNQANWDSRAPIHAAAYGIEGLLADPAALSAVVRFDL
                     PRLGNLAGLDVVHLQCHIGTDTLSLSRLGAQVTGVDLSPVSLEQARRLAAAADADIDY
                     IEADVYSAPEALAGRDFDLVYTGIGAICWLPSIQRWAQTVADLLRPGGRLFIRDGHPV
                     LLALAPVVLGADPDDRVQQPWMTAAGARTVALEFPYYERAEALVWHDEHTYAGEDLVG
                     SPESMEWNHSLGEIVTAVLEAGLTLTGLTEHQSVPWEALPGLMELDPEVDEYRLAERP
                     ERLPASFTLTAVKPL"
     gene            complement(42330..44450)
                     /locus_tag="PKJ61_01250"
     CDS             complement(42330..44450)
                     /locus_tag="PKJ61_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase UvrD2"
                     /protein_id="HOC12491.1"
                     /translation="MPEPKVCKVITDPTALLVGLDPEQRQVATAFGQPVVVIAGAGTG
                     KTRALTHRIAYASAIGAFNPNAVLAVTFTTRAAGELRSRLRGLGVPAVQARTFHSAAL
                     RQAQYFWPRAYGSALPEVTAERLPIAAAAARKLRLSPDAATLRDLLTEISWAKVTNVP
                     AADYADLATAQGREVSGLDPAQVGRVLGRYEQVKRDRGVIDFDDILLCATALLSEFDD
                     IADEVRGTYRHLVVDEYQDVNPVQQTLLDLWWGEGRDHCVVGDPAQTIHSFAGASPGY
                     LTGFASRYPEAHVVRLVRDYRSSPQVVELANRMARNTRLGTVTLESQCPPGPQPRILP
                     CASEHDEATAVARWLIEQHEAGVPWRDQALLYRVHAQSPLFEAALTEAEVPFTVRGGE
                     GFYERPEVRQAIGTLAEQARRDPSVPAISACEALLARLGWTGTPPAGQGRVRERWESL
                     AAILALAEDVNTAVADAGGGGGATIGEFVAELAERAQAEHPLAAEGVTLSTLHAAKGL
                     EWTAVALVGVQEGTLPFALAAGPEQVAEEARLFYVGITRAKRALHVSWSRARRGGGGG
                     FRQPSRFLDGLAARPSTGPVRVRRGPKPVSAMGAACQVCGKPLTTGAERKLRRHAACP
                     AGYDEETYARLVAWRADVARMRAQPAFVVFTDATLMVIAESRPRTASELLAIPGVGKV
                     KCEQYAEAVLAILSEEVAEHQEQT"
     gene            44492..>47037
                     /locus_tag="PKJ61_01255"
     CDS             44492..>47037
                     /locus_tag="PKJ61_01255"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:NF016169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HOC12492.1"
                     /translation="MQVQHARTAEDLVSRLTSAWSGPRPDPFAFDLAVVPGAGFQRWL
                     SQQLALSDAGICAGVEFVSLPAFERRLGGTDDPWQPDRLVWRVQRLALEPDLPELETL
                     RRHLLASRESYTACNRIARHFAGYARHRPKLLAAWTRGEDAAPDGQPLGDDAWQAQLW
                     RRLVAEIGESPLDRRLALLNRLRSGPTPGLPERIAVIVPTQFHAGLLELLEAVGSHHR
                     VELLALCPTPSRRPARVTAELRRADFTRPVGHRLNLSLGQLGDEAAALFPPVTSTTAP
                     ESIPSLLGWLQANLRDDTEAAPRALDPSDDSVQVHLSHDLSRQVEVLREVLTSVLAAD
                     PSLEPRDIAVITPDVDAVAPLIGAAFTVAGGPSHPATRFRVQLADRSVAQVNPMATLL
                     LRLLALPDGRFEASTLLELCQEPAIAARFGFGPDARERLVRLVETAGIRWGLSAQQRA
                     GFGLGGFPHNTWFAGLQRMLLGVALPETDLVAAGTVLPLDDVESSDVALIGGLTEFVG
                     RLSRALNELSATGTLAEWCARSRSLLESVVSLPHGQEWQLSDLWTGLSRLAERGEQPG
                     APLTRAAAERAIADEFAGAPARGAFGNGSLIVCGPNSLRHVPHRVLVLLGWDADRYPR
                     PVRRHGDDLLGQEPRVGDPSGALLDRQLLLDAVHAARQQLIVVAQGRSVATNEVVPLA
                     APIAELVAALDATAVTAAGLGAGAAVTVQHPLQSFDARYFGGDARLYSADPLAFRAAR
                     ASVAEPEAPRPRYYLETLPPPDLSQGVTLEQVTDFFKHPARALLKVRAGLSLGNSAES
                     SDELPIELDHLERWQIGNRVLQRLRAGHDADAVRRAEWLRGEVPPFELGR"
BASE COUNT         7421 a        16196 c        16043 g         7277 t
ORIGIN      
        1 tcgcggttgc tctgggtctt gcgcatgcgg ttgagcagca tctccagacc tgcctgcgga
       61 tcctgcgcgc tgagcactcg gcgcagcttc cagatgatcg ccaactcgtc gcgggccagc
      121 agcagttcct cacgccgggt gccggaggcc accgggtcga tcgccgggaa gatgcgcttg
      181 tcggcaaggt cgcgccgcag ccgtagctcc atgttgccgg tgcccttgaa ttcctcgaag
      241 atcacctcgt ccatcttcga tccggtctcg atcagcgccg tggccaggat ggtcagcgag
      301 ccgccgtcct caatgttgcg cgccgcgccg aagaacttct tcggcgggta gagcgcagcc
      361 gaatcgacac cgccggacag gatgcgtccg gacgccggcg cggccaggtt gtaggcccgc
      421 cccagccgcg tgatgccgtc cagcagcacc accacgtccc gcccgagctc caccaggcgc
      481 ttggcccgct cgatcgcgag ttcggcgatc gtggtgtggt cgtcggcggg acggtcgaag
      541 gtggacgcga tcacctcgcc ggacgtcgtc cgctggaagt cggtgacctc ctcgggacgc
      601 tcgtcgacca gcacgatcat gaggtgtacc tctgggttgt tcaccgtgat cgcgttggcg
      661 atcgcctgca taaccagcgt cttgccggcc ttcggcggcg acacgatcag gccacgctgg
      721 cccttgccta ccggagacac gaggtcgatg attcggccgg tcatgttgat tccggtggtc
      781 tcaaggcgca gccgctcctg cgggtacagc ggagtgagct tctggaagtc gggacggtcc
      841 ttggccttct ccggcgctgc ggcgttgatc gtgtcgatcc gcaccagcgg gttgaacttc
      901 tcccgccgct cgccctcctt cggcgcgcgg atggcaccgg tgacgatgtc gcccttccgc
      961 aggccccact tcttgaccat cgacagcgaa acgtaggcgt cgttgggtcc gggcaggtag
     1021 ccggtggtcc gcacgaatgc gtagttgtcg agaacgtcca ggatgccgga gatctcgatc
     1081 accacatcgt cttcggccag caccggctcg gcttccatcc ggtcgagacc ggcgggcccg
     1141 ccgacggcac ggccaccgcg acggttgttg ttctgccggt cacggttgcg gcgacggcgg
     1201 ctgcgccggc cactgccgta atcgtcgtcg tcgcgatcgt tgcggtcatt gcgctgctgg
     1261 ttctgctgcg attgcgagtt ctgctgcggc tggcgcgccg tttcactgtt gcgagcctcg
     1321 gcaccgtcgg ccccgtcccc acgattgcgg cgcggacgga tggactcctc agccttcagc
     1381 gcatccagtc gggctgcgac atcgtcaacg ccggcgctgc ctgcgtcagc ggtgccggaa
     1441 tcggcacggg ccgcaggctc acgatcggtg gtctgggtgc gctcagcctg gttgccgcgc
     1501 gcccgggaac ggcgggccgg cgccgcttct gccgtccgtt cggcagaggc ttcgccgacg
     1561 gtcgcgtccg cctcggcgct gcgcgggagc gtgggctggc cggcgtccgc ggcctcgccg
     1621 gacggtgcgg ccttggcgcg ggcgcgacca ccctttggtc gctcgctggc cgggcgttca
     1681 ggcttggccg agccctgcgc ggtctggatc gcctcgatca gcgcaccctt gcgcatggtg
     1741 ccggtgccct tcagcccgag ggaagcgccg agctgcttca gctccggcag cagcattccg
     1801 tcaaggccag cgccggagcg cttgcgggta ccggcttcga tggtgtcggt cacgggtttc
     1861 ctttcaatcg ggcgtccgcc ggcatggccg tacgggctcc gcccggtgcc cacaaagagc
     1921 tcttgtttga cagccagatc cgagatgcca ggatgctctg aacagctact gggatctctc
     1981 gacgagtgag gacgtctgct tcgcgttcgt cttgcccgat ctgaggggca gccacgcctg
     2041 aggcgcgaag ccagcttagc acccggcggc gagcgcaggc gcagagctgc cgaatatctt
     2101 cacgtcgagg ggaaatctgc tgcccagctg tggctagagg gccgagctga acgcgttgcg
     2161 cagcccggcg tagtcggtga ccaccgggaa ttgcgggaac tcggcgacca cattggccgg
     2221 tggccggaac aggatgcccg cgtcggcttc ggcgagcatg gcggtgtcgt tgtaggagtc
     2281 cccggtggcc accacccgga agttcagctc gtgcagcgct cggacggcgg tccgcttcga
     2341 ttcggcctgc cgcagggtgt agccgatcac gcggtcgtcc tgcacctgca gccggtggca
     2401 cagcagggtc gggaagccga gttgggccat cagcggcatg gcgaactcat agaaggtgtc
     2461 ggagaggatc agcacctgga agcgttcccg cagccagtct aggaactccg ccgcgccggg
     2521 catcggtccc atttcggcga tcaccgcctg gatctgggtg aggccgatgc cgtgcgcgtc
     2581 cagcacgcgc agccggtggc ccatcagctc gtcgtagtcg gcgatgtccc gagtggtcag
     2641 ccgcagttcg tcgatcccgg tggtctcggc gacattgatc cagatctccg gaacgaggac
     2701 gccttccaga tcgaggcacg ccagatccat caggccccct gcacccgcat cacggcgatg
     2761 ccgggatgca cttcgggcat tccgcccagt tcggcgatgg ccgcgtccgt ctgctcaccg
     2821 agcgcgaggt gggacatgac cccgaggcgg gcctgtgcac cggtgctctc ctggcggacg
     2881 gcctgcagcg acactccgtg cttggcgaac gtcgcggcca ccttggccag cacgccgggc
     2941 tcgtcgtgca cggtgagcga aacgtagtag cgcgaggcaa ccttgccggg cggcgtgatc
     3001 gcgcgtcccg aataggtcga ttcggccggt ccccgagcac cacggacgcg gtttcgcgcg
     3061 gctgtcacca ggtcacccag caccgcggac gcggtcggcg acccacctgc gccgggcccc
     3121 atgaacatca gccggccggc gtgccgcgac tcgatgaaga ccgcgttgta cgcacctgga
     3181 atcgcggcca gcgggtgcgc caccggcacc atcgtcgggt gcacgcccac cgcgatggtg
     3241 tcgtcgtcac tgagcgtggc actggccagc agcttgatca cgcagcccat ctccttggct
     3301 gcggcgatgt cctcgccggt cacgccggtg atgccctcgc gatccacgtc ggcgaggttc
     3361 acccgggtgt ggaaggcgag actggccagg atcgcggcct tggctgcggc gtcgtagcct
     3421 tcgacgtccg cggtcgggtc ggcttcggcg aaacccagtt cctgcgcctc ggcaaggacc
     3481 gcgccgaaat cggcggaatc ggtgtgcatc ttgtccagga tgaagttcgt ggtgccgttg
     3541 acgatgccga ccaccgcggt gacctcgtca ccgaccagcg actcgcgcaa cggccggatg
     3601 atcgggatcg cgccggcgac cgccgcctcg tagtagacgt ccacaccggc ctgctcggcg
     3661 gcggcgtaga tcgtcccgcc gtcctcggcc agcagtgcct tgttcgcggt gatcaccgaa
     3721 gcgccggcgc cgatcgcagc caggatcagg ctgcgcgcgg gctcgatgcc gccgagcagt
     3781 tcgatcacca ggtcgacgtc ggtgcggctg gcgagggcga gcgcgtcgtc ggtgagcagc
     3841 gcgcggtcga tgccgggccg ctcgacttcg agccggcgca ccgcgatacc gaccagttcg
     3901 agcggacgtc cgatccgggc gcgcaggtct tcggcctgtt cgatcagcaa gcgggcgacc
     3961 tgggagccga ccactccgca gccgagcagg gccaccttca gcggcgtgga atccgtcatc
     4021 atgcttcctc gtcagcgagc ccggcgtcga gcgccaggag gtcgtccagg gtttcgcggc
     4081 gcaccatgac ggagaccgtt ccgtccttca ccgaaaccac cggcggccgc ggggtgtgat
     4141 tgtagttgct ggccatcgac cggctgtacg ccccggctgc gggcacggcc agcaggtcac
     4201 ccacggccac gtccgcgggc aggaactcgt cgcgcaccag gatgtcgccg ccctcgcagt
     4261 ggacgcccac cacccgcgac accaccggcg cggcggcgga ggtgcggttg gccagcgctg
     4321 cggagtactc cgccgcgtac agcgccggac ggatgttgtc gctcatgccg ccgtccaccg
     4381 aaagatagcg gcggctcagt cccccgccga cgtcaacggg cttgaccgtg ccgacccggt
     4441 acagcgccat gccggacggg ccgacgatcg cgcgcccggg ttcgatggag atcgccggga
     4501 cggccagcct gaagcccgcg cactcgtgcg ccacgatctc ccgcaacccg gacgcgagct
     4561 cggacgggga cgccggcgtg tcctccttgg tgtaggcgat gccgaagccg ccgccgaggt
     4621 cgagctcgga cagctcgatg ccggtggcct ccttgaactg ggcgaacagc ttgagcgtgc
     4681 gccgggcggc cacctcgaag ccgttggtat cgaagatctg gctaccgatg tgggaatgca
     4741 caccgagcag gttcagccgc ggactgtggt ggcagcggaa catgccgacc agcgcttggc
     4801 cgccggtgat cgagaagccg aacttctggt cctcgtgggc ggtggcgatg tactcgtggg
     4861 tgtgcgcctc gacgccggtg gtcacccgga tcatgacgtt ggctgtggtg tccagctcgg
     4921 cacaaagcga ttcgaggcga gtgatctcgt ccgaggagtc gacgatgatc cggccgacac
     4981 cgcgaaccag ggctagccgc aactcggctt cggacttgtt gttgccgtgc atgccgatcc
     5041 gggctgggtc gaagcccgct cgcagcgcca cggtcagttc accgagcgag cagacgtcga
     5101 ggttgagccc ctcctcgccc acccagcggg cgatgacgcc gctgaggaaa gacttgccgg
     5161 cgtagaacac cgtccagcct tcgaaggcgt tgcgccattc gcgcgcgcgt tcgcggaagt
     5221 cgtcctcgtc gatcacgtag gcgggggtgc cgacctgctc ggccagctcg gtcaccggaa
     5281 tgccggcaac ctggatcacg ccctcgtcgt cgcgcttgac ggtcttgctc cacagcttgt
     5341 cggagagggc attgacatcc tcgggccggg tcagccacac cggtgcgggc gtggtgacgt
     5401 cggcgtggat ggagccggcg acgtgcatgt gggtcatggc aggcaactgt gccagaagtg
     5461 gccgctcagc tgccgctggc cgcccggatc tggacgtcgg acacctcggc caggcggagc
     5521 tctgccggtg tcgcaaccgc ggcaccgtcc gcgctcacct ggacgccgcc gcacgctgca
     5581 ccgaggcagc gctcatcgaa gcggacgccc cagatcgtga agaattggcc gagcgtgacg
     5641 tcctggacgc cctggccctc caaccagacc gtgccgctgg catcgtgggt gtgcaccggc
     5701 gcctggagcg cgcgcggacg gtcgacaccg atgttcggca ccaacgggac gccgacgtcg
     5761 tcgatgctga tcgccacggt cacgacccgc tggttcgagc ggtcgtccag acgggacgcg
     5821 ggcacacccg ccgcctcgaa atacgcgatg ccgtcgcgcg gtgcgggcca cggcggtggg
     5881 gtctggcgca gttcggccgg ggccggcgcg gccttggcca cgcaaccgga cagcaggacg
     5941 agcagcagcg ccgctgcgag cagtctgcgg gccccggaca cggccacagg ataggcccgc
     6001 gagacgcacc gcgaatcttt gctaaggtgt ccaagcccgc aacgcgggtg tgcccccgta
     6061 gctcagggga tagagcaccg ccctccggag gcgggagccg aggttcgaat cctcgcgggg
     6121 gcgccacatc ggtcccggtc agagacactc tggccgggac cgcttacttg atgccgacaa
     6181 gctcgaccac ccgaaacccg accgaccggc cgcagctcga tcgatcatcg gagttccatc
     6241 gactggtcag acctcgattg accagccgag ccgtgggact cgccgccttg ttcgttcaaa
     6301 tccggtggtg gttgtaggta gcatcaccca agagccgaaa agacagcgtg gacaaggaag
     6361 aaggcgactg tggccacacc tggttcgggg gtcgacacga acgattcatt ggccgaattc
     6421 ggcgcaaacg actggctgat cgaggagatg cgcgagcagt atctcgccga ccccgagtcg
     6481 gtggacgcaa gctgggtcga gtacttccgc agtcaccaag gtgcttcggc ggacgggacg
     6541 caaccggcgg ctgcaccgtc cgcggctccg accgcccccg ctgcggcgac ccaagctccg
     6601 gaagtccaag ctccggcgac ctccgctgcc gcagagaccg tgcaggtcgt gcaagtgccc
     6661 atcgtggacg tggccgaacc cgagccggag tcccgtccgc tggccgaagc cccggtgaac
     6721 gagccggcag tcgaggccgt ccagccgaac gcagagaacg cgccccagat cactggagca
     6781 gcctccggcg tatccgcgaa ccgcccgaag caggcgccgc agccaaccaa ggatgcaacc
     6841 gagcccgtcc gcaccccgct gcgcggcgcc ccggcccgca ccgcggtcaa catggacgcc
     6901 tcactgtcga tgccgaccgc gaccagcgtc cgccaggtgc cgatgaagct ggtgatcgac
     6961 cagcggcaga tgatcaacgc gcacctggcc cgctccaccg gcggcaaggt ctccttcacc
     7021 cacctcatcg gctgggccat ggttaaggcc ctgctggccg tgccggagat gaacaactct
     7081 tacgaactgg tcgacggcaa gccgaacctg gtgacgccgc cgtcgatcaa cctcggcctc
     7141 gcgatcgact tcaagaaggc ggacggcacc cgccaactcc tcgtaccgaa catcaagaac
     7201 tgcgaggacc tcgacttcgc cggcttctgg gcggcctatg aagaactggt tggcaaggcc
     7261 cgcaggggcg agctgaccgt tgctgacttc gccggcacca ccgcgacact gaccaacccg
     7321 ggcggcatcg gcaccaacca ctcggtgccc cgcctgatgg ccggtcaggg cctgatcctc
     7381 ggcgtcggct ccatcgacta cccgccggag ttccagggcg ccagccaggc ccggatcaac
     7441 caactcggcg tttcgaaggt gaccacgctc acctcgacct acgaccaccg ggtgatccag
     7501 ggtgcacaga gcggtgagtt cctgaaggtg ctgcacgagc tgctgatcgg tcagcatggt
     7561 ttctacgacg agatcgtcca ctccctgcgc gtgccctaca ccccgatccg ttgggccaac
     7621 gacgtcaatg acgaacgggt cggctcgatc ccgcgcagcg cacgaatctt ctcgctgatc
     7681 aacgcctacc ggaacctcgg tcactacatg gccgacatcg acccgctgga ataccggcag
     7741 cgatcgcacc cagagctcga cctggagtac cacggcctaa ccctctggga cctggatcgc
     7801 gagttcccgg ttggcagttt cggcggccga agcggtgagg tgcacacgct gcgcgacctg
     7861 ctggtgatgc tccgcgactc ctactgccgc actgtcggtg tggagtacat gcacattgcc
     7921 gacaacgagc agcgcacctg gatccaggac cgcttcgagc gtccgttcgt gccctggccg
     7981 cgcgaggagc acctgcggat cctggacaag ctcaacgagg cggagatctt cgagaccttc
     8041 ctgcagacca agttcgtggg acagaagcgg ttctccctcg agggcggcga gtcggcgatc
     8101 gtgttcctcg acgaggtctg cgagcaggcc gcggacgccg gcctgagcga ggccatcatc
     8161 ggcatgcccc accgcggccg gctgaacgtg ctggccaata tcgtgggcaa gtcctacggc
     8221 cagatcttcc gcgagttcca gggcaactac gacccacggc tctctcaggg ctccggtgac
     8281 gtgaagtacc acttgggctc cgaaggagag ttcaaggcag ccagcggccg gacgatcaag
     8341 acttcggtgg ccgcgaaccc gtcccacctg gaggcggtcg acccggtcgt gcagggcatc
     8401 gcccgggcga agatcgacca gatcggcaag atcgagggct ggccggtgct gcccatcctg
     8461 atgcacggcg acgccgcgtt cgccggccag ggcgtggtct acgagaccct gcagatgagc
     8521 caactgcgtg ccttcaagac cggcggcacg gtgcacgtcg tggtgaacaa ccaggtcggc
     8581 ttcaccaccg cgccgatcga atcccgctct tcggtctact gcacggatgt cgccaagacc
     8641 gtgcagtcgc cgatcttcca cgtcaacggc gacgaccctg aggccgttgc ccggatcgcg
     8701 caggtggcct tcgacttccg acaacggttc gccaaggacg tcgtcgtcga catcgtctgt
     8761 tatcgccgcc gcggccacaa cgagggcgat gaccccagct tcacgcagcc gctgatgtac
     8821 gacctgatcg agcagaagcg gagcgtccgc aagctctaca ccgaggccct gatcggtcgc
     8881 ggcgacatct ccaccgatga cgccgacgag gtcatcgcga agttccgcga tcgcttggag
     8941 agcgtcttca aggaggtaag ggaagagccg caggacgagc cgatcagcaa gacgccctac
     9001 tacccggcga agctcggcaa ggacgtcggc acggcgatca cggctgaggc gatggaggcc
     9061 gtggccaagg cccacctcac cttccccgag ggcttcacgg tgcacccgaa ggtgctgccc
     9121 cagctggagc gccgcgccga cgccatccgc aagggcccga tcgactgggc gaccgcggaa
     9181 ctgctggcgt tcggttcgct gctgatggaa gggcgcccgg ttcgcctggt cggccaggac
     9241 agtcgccgtg gcactttcag ccagcggttc gcggcggtca tcgaccgggt ggacaaccgg
     9301 gcctacgtcc cgctgaagca cctcagcccc gaccagggcc gtttccacgt cttcgattcc
     9361 ctgctcagcg agtacgcggg gatgggcttc gaatacggct actcggtggc cgccccggac
     9421 gccctggtgc tctgggaggg ccagttcggc gacttcgcca acggcgccca gaccatcgcc
     9481 gacgagttca tctcctccgg caacgccaag tggacgcaga agtccggggt ggtgctgctg
     9541 ctcccccacg gctacgaggg ccagggtccc gaccattcgt ccgcccggat cgagcgctgg
     9601 ctcaccctgt gctcggaggg ggcgcttgcg gtgtgccaac cgtccacgcc ggccagctac
     9661 ttccacctgc tgcgcaccca cgcctacgtg aactggcacc ggcccgtggt cgtcgccacg
     9721 ccgaagtcga tgctgcgctc gaaggcctcg atgtcgccgg tggaggagtt cacccaggga
     9781 aggtggcaac ctgcccaggg cgatccgacg atcaccgacc cgagtgcggt gcggcgcatc
     9841 ctgctgtgct cgggcaagat ccgctgggag ctcacggcag cgcgcgccga agccgggttg
     9901 gacggccaga tcgcgatcct gccgttggag cgtctctacc cgctgccgac caaggaactg
     9961 gccgctgagc tggccaagta cccgcaggtc accgacatcc gctgggtgca ggacgagccg
    10021 gagaaccagg gcgcctggtg gttcctgcag ctgtacctgg ccgacgccgt ccgcacgatt
    10081 ctgccggact acgagctgcg catgaccggg atcacccggg ccgcggcgtc cgccccctcg
    10141 gtcggatcgg tgaaggtgca ccaggctcag gaagcggacc tgctcgcccg cgccatggcg
    10201 gactgatgta cttcaccgac cgcggtatcg aggagctcgc cgcccgcagg ggcgatgagg
    10261 aagtgacgtt ggcctggctc gccgagcagc tgagcacttt cgtcgaccta tggcccgagc
    10321 atgagaacgc ggtcgagcgg ctggccacct ggctggcaag actcgacgac gaggaggatt
    10381 agcgtgttga cccaggctgc agtggaggcc ttcgaggagc tcaccggatc ggcaccggcc
    10441 ggtgtatggg ccgcgcccgg gcggatcaac ctgatcggcg aacacaccga ctacaacgac
    10501 ggcttcgtgc tccctttcgc gctcccgcag gcagcctacg tggccgcttc gcggcgggag
    10561 gacggccgct ggcgcttccg ctcggtcgat ctggacgaca cggtggagat cgacgcggac
    10621 gggctggttc cggggactga cgggtggcat tcctacctcg ccggggtgat ctgggcgctg
    10681 cggcaggcgg gccacgaggt tgggggtgcc gacctggtgc tcacctcgga cgtgccgctc
    10741 ggggctggac tgtcctcctc ggccgcgatc gaatgcgccg cactagccgc gctggccgac
    10801 ctttacgacc tgaccatcga gccgatggac cgggcgaagc tcgcccgggt cgcagagaac
    10861 gcgtacgtgg gtgcacccac cgggctgatg gaccaggccg cgtccacgtt gtgcgtcgcc
    10921 gatcacgctt tgttccttga ttgccgcagc ttcgacaccc ggcaggtacc gctggcagtc
    10981 acccaggcag ggctcgagat ccttgtgatc gacacccgca cccggcacag ccacgtgagc
    11041 ggtgagtacg ccgaccggcg cgccagttgc gaggaggcct gccggctgct cggcgtggcg
    11101 gccctgcgcg acgtcaccga cctggacgcc gccctggcgg tgctgccgga ggagatcggc
    11161 cgcagggtgc ggcacgtcgt gaccgaggat cgccgcgtgc aggactcggt ggcggcactg
    11221 gatgcggaca atcacgccca gcttggcgcg ctgatgacgg cgtcccacga ttcgatgcgc
    11281 gacgactacg agatcaccga gcccagcgtc gacctcgcgg tcgcaaccgc gctcgcgtcc
    11341 ggtgctctcg gggcccggat gaccggcggc ggcttcggtg gctgcgtgct ggccctggtg
    11401 gccgccgagc aggcggaggc ggtcggcgcg gcggtgacgt cggctttcgc cggggcggga
    11461 cacgccgagc cggtcacttt cctggctcgt ccgtccgctg gagcccggcg cgtccgctga
    11521 ggtgcaacgc ccggctggtg gacggtagcg cggccgcgac cacggtggcg gccgccacca
    11581 gcaccaccag ccacgcccaa ggcgcacctg gggtgaaact ggccgcagcg atcaggttca
    11641 gcagggcggt cgcgatcacc ggtcccctgc cgaacaggct gccccgccag agcgcccagg
    11701 tggccgcgag gatcagcaat ccgtagacca cgagggcgat acccaccgcc ccgctgaact
    11761 cgccgtgccc ggccgccagc gaggcgatcg ctagcgcgag aatggccagc ccgaccagac
    11821 cggaaccggc caccgcgacc agcagcccgg ccggacgctc cagtgttgct ctagacacgc
    11881 cggccagcct agcgggggtg cgcacgcccg aaccgagcgt gcacgcagtg atagcgcacc
    11941 acacaacatg tgcaaattgc ggccaagacc ttgtgctgac cggctagagt tgccactctg
    12001 gacgccgggc gttgagcaat tctcgcgccc ggtgacgtgt gcttgggccg acgtcacggc
    12061 ccaccgaaca ggagtgttac ttcatggatt ggcgccatca ggctgcctgc ttgaccgaag
    12121 atccggagtt gttctttccg gtcggcaaca cgggaccagc gttgatgcag atagcggagg
    12181 ccaagaaggt ctgccgccgc tgcgacgtac gcgaggcttg cctgcagtgg gctctcgatg
    12241 ccggtcagga ccatggagtg tggggtggtc tcagcgagga cgaacgtcgc gccatgaagc
    12301 gtcgcgccgc gcgcatgcgc gtccgcaacg gctgatcctg accgaccggt cgagcccgtc
    12361 gagaccccgg cctgcgtcag cgcagcggca cccggacggt ggccgtggtc gcatcatcat
    12421 cggacgcgag cgtgaactga cccccaaggt cggccaccag cgtgctcacg atcgagagcc
    12481 ccaggctggt cgaccggccg atgcggaagt cctccggtag gggctcgccg aagttcttca
    12541 ccaccacgtt gagttcgccg tccgactgct gcgggatcac cagcacctcg ccggccttgt
    12601 cccgcaggcc atgctcgatg gcgttctggc agagctcggt gagcaccagc gaaaggctgg
    12661 tggccacgtc ggcgggcacg tagccgaacg agccctggag caccatccgc accttgccgc
    12721 cggtggcggc cacatcgccc accatgcgca gcagccggtc ggccacgtcg tcgaacttca
    12781 ccgaggagtc gaaagcctgc gacaggattt cgtggaccac cgcgatcgca gcgacccgct
    12841 tcatcgcgtc gctgagggcg cccttggctt cgggtgactc gatccggcgg gcctgcaggc
    12901 gcagcagcgc ggccaccgtc tgcaggttgt tcttgacgcg gtgatggatc tcgcggatcg
    12961 tcgcgtcctt ggtcaccaac tggcgttcgc gggaacgaag ttcggtggtg tcgcgacaca
    13021 tcaccagcag tccgtccggc ccgtcctcgc cggtgagcgg gagaatgcga atgcggacgt
    13081 tggcggtcgt ggtctccagg tcgagctcgg ccgagccgcg accggcgagg ctcgcggcaa
    13141 ccggacgttc gaccgggacc ttctcccgca gcagcgagcg ggtgaccgcc acgaagttct
    13201 cgccgtagag atcgctggtc aggcccagcc ggcggtaagc gctgagcgcg ttcgggctgg
    13261 cgaaccggat catgcccgcc tggtcgaccc ggatcatgcc gtccccgacc cgcggtgaga
    13321 cccacggcac ccgccggtca ccgcccagcg ggaactggtg ccggtgcacc atcccggcca
    13381 acacctgcgc agcctccagg taggcgtcct caagtgcccc tggggcacgg acgcccatct
    13441 ggttggtgtg catctccaca acgccaagac agcggtcgtc cgccaggatc ggaatcgcct
    13501 gcacgtccac cgggatgccc gcatgcagct tgttgccgct ggtgtggctg atctccctgg
    13561 agaggtacgc ctcggtgacc aggtgctcgg ggtcgtaggc gatctcctcc cccaccacgt
    13621 catcctccag tgaggtgggc ccggtggtcg gccgcacctg ggcgcaggcc cagaacacgt
    13681 tgtcgtcagc gtcgggcacc caaaggatca ggtcggcgaa agcaaggtcg gcgagaagcg
    13741 accattcgtc caccagcagt tccagccact cccggtcctc gtcttcgagg acgcagtgtc
    13801 gggcgatgac ctgctccatg gtctgcatga ccgccaccct aaccgacttc ggttggactc
    13861 cttcgcgcgt gggctggcag aatgggccct cgcgccgctc ggcgcgagtc ccgagtcgac
    13921 ttcaaggaga ccacgatggg taagggtggc cgcaagcgcc gttcccgcag gaagagcgca
    13981 gccaatcacg gcaagcgccc gaacgcctga ggatcgccgc tgtaatcgaa aagcccccgc
    14041 cggttcggcg ggggctttcg cgtgttcatc cgtccccgct tcaggcgtcc ctggtcaggc
    14101 ggacgcccgg tcggcgcacc gcgtccgctg gttcccgcca gcgaaactga cgaatgcctg
    14161 cgaagagtcc ccggcgtctg tcagtttcgc cggcgggaaa taggtggggc cgggcgggcc
    14221 ggcgaatcga gcgggaccgc gcagggtcgg aggggctagt cggtcagctc ggtgatgagg
    14281 atcgtctggg agtagatggc ccggacccgc aatcgcagcg actcgggtgc cgactggcag
    14341 caggagcggc gaaccagcat ccgcagcgcg gcctcgacgt cgtagtggtc caggcagccc
    14401 tcgcaagcga gcagatggtg gcggatctcg tccgcctcgg ccggagtcaa ctcgtggtca
    14461 tggaacgcgt acagattgcg cagcacctcg gtgcagtctt cgaactcact caccatcaga
    14521 gtccccgatc agtccgtgct cgcgggcgaa gtcggcaagg ttggcgcgca gctgattgcg
    14581 gccgcggttc aaccgggaca tgaccgtgcc gattggagtg cccatgatct cggcgatctc
    14641 cttgtaggcg aaaccttcga cgtcagcgag gtagaccgcg agccggaagt cgggagccag
    14701 cgcgttcatc gcgtccgtga ccgcggaatg cggcatccgg tcgagcgctt ccatctccgc
    14761 cgaccgcagt ccggtcgagg tgtgcgcctc ggcgcgagca agctgccagt cctcgacctc
    14821 ctgcccggag tgctgcggct ggcgctgctt cttccggtag gtgttgatga aggtgttggt
    14881 caggattcgg tatagccagg ctttgaggtt cgttcctggg gtgaactggt ggaaggaggc
    14941 gtaggccttc gcgaacgtct cctgcacgag gtcttcggca tcggacgggc tacgggtcat
    15001 ccgcaaggct gcgccgtaaa gctggtcgag gtaggccaga gcctcagtct cgaagcggtg
    15061 gtcgcgctcg gccttgctct cgtccaaccc ggtatcggtt tcgctgggct ttgtcggcag
    15121 gttcatcacg aaggagccta gcgggtgctc ctggcgccgg cggacgtctg ctgccaccgg
    15181 ttggccaagg gtgccgatca tgcttagtga acggaaccgg acggctctgc tattcccgga
    15241 ccgctcggtt ggtcagctca gcggcgtgat caactccggg ccgtcgttgg ccaccttgtt
    15301 cacggcggtc gagaccgggt agtaggtaag gcttggcgcc ggcacggtga gcagagcgcg
    15361 cgcgtccatg ccgaagcccg ggtcgagcca ggcctcccag ttctccggcg caaccaccat
    15421 cggcatccgg tcgtggatat ggccgacctc gtcggcagcc gcggtcgtga tcacggtcgt
    15481 ggtggccagc caggtaccgt ccggggcctt ccagaactcg tagagcccgg ccatcaccag
    15541 cagcccgcct ccggccggac ggatgaagta gggctgtttc gttttgctgt cggggagttg
    15601 gtaccactcg tagtaaccgt cggcgggcag caggcaccgc cgcgccgcga aggccctgcg
    15661 gaaggccggt ttctcggcga gcgtctcgac tctggcattg atcaggcgag cggcgccggt
    15721 ggcgtccttc gaccaggacg gcaccagccc ccagcgcacc tcggccagca cccgtcggcg
    15781 gccgtcggcc cggtcgcgct ccataatcgc cggcacgggg tcggtgggcg caacgttgta
    15841 gtccggcccg ggcaacggtc cggccacctc gtcgatctcg aagatctcgg tcagttcgtc
    15901 cacatccgcg ctcaccgcgt accgtccgca catgccgaag aggctagcgc gcagaatgtg
    15961 cgctgaaact ccccagttgg gtcgcgccga gggcaatgat cggggtagga atagggcatg
    16021 agtgttctgc ggttcgtcgc ccgttcgatg ctggccagtt acttcgtgct caacggggtg
    16081 cgcgccttgg cgcaccctga agacctggca cccgccaccg ctccgatcgc ggagaagctg
    16141 atgccgaagt tgtctgccgc gctgcccgaa tccgcgcgcg ctttcgtgcc caccgacgcg
    16201 accggcctgg tgaagctgaa cggcctactc cagatcgtgg gcggggcgag cctggccacc
    16261 gggttcttcc ggcgaatcgg cgccggggtg ctcgcggtga ccaccgtgcc caacctgctg
    16321 gccgcgaacc cgctgaccaa gggtccgggc caggccgcgg cccaagcgga gttcggtacg
    16381 aacgtggccc tcctcggtgg cgtgctgctg gccgcccagg acaccgaagg caagccgaca
    16441 ctggtgtggc ggctgcgcac gcagcgcgag ttgctcgcca aggacgcggc caagcgcaag
    16501 gccgaacttg agcgcgacgc gcgggccacc gcagcggacg cggccaagct cgcccgcaag
    16561 tcggtgaagc agggccgcaa gttcgtcgcg gaagtgcttt cttgagcgct tggacggcac
    16621 cggtcgcgac cgctggagtc cgtggccacg tagctgttcc gggttcgaaa tcggcaagtg
    16681 cgcgcagcct gctgctggcc gcgctcgctg atggtccttc ggttctttct ggcgtgctgg
    16741 attcgcgtga caccaccctg atgcgggtcg ggctgagcac gctcggcgcc gcgttccatg
    16801 acctgccgga cgggcggttg gaggtccgtc cggcggtggc agtgcaaggt ggtggcgaga
    16861 ttgactgcgg cctcgccggc acggtgatgc ggttcctgcc tccagtggcc gcgctggcgt
    16921 ccaggcccac ccggttcttc ggcgacccgc acgcttccgc ccggccgatg acgcccttgt
    16981 tgggcgctct tgcgagcctc ggcgcagcag tgagtacgcc ctatcagctg ccgttcacgg
    17041 tggcttccgg tgctttccat ggcggctcgg tcacggtgga cgcgagcggt tcgagtcagt
    17101 tcatctccgc tctgctgctg gccgctgccc gctttatcga cggcatcgag gtgcgccaca
    17161 ccggcgccac cctgccgtcg ctcccccaca ttgagatgac ctgccaactg ctggaacgtc
    17221 gcggcgtggc ggtgacgcgg ccgggcatga acacctggcg ggtcgcgtcg gggccgatcg
    17281 cagcgctcga cgaagccgtt gagccggacc tgaccaacgc ggcgacgctg ctggccgcag
    17341 ccctggtgac cggtggcgag ctcaccacgt cctggccgga cggggtgcaa gccacgtctg
    17401 cgctggccga cgctttggct gcgttcggtg ggcaattgag ctacaacggc gcaggtgccg
    17461 atcgagccat cacgctcagc ggcgcgggcg ggctacgcgg tgcggacgtc gacctcgccg
    17521 ccatcagtga gttcaccccg gcagcagccg ccctggctgc cctcgcaccc gggccgagcc
    17581 gtattcgcgg cgtggcgcac atccgcggtc acgaaaccga tcgactggcc gcactcagca
    17641 ctgagctggg ccggttgggc gcagcggtgc ggcagaccgc ggacggactt gagttcgctc
    17701 ccgccacctt gcatggcggc agcttcgcaa cccatgcaga tcaccgcatg gcccacgccg
    17761 gtgcactgat cggcctggtc acacccggag tcgagcttga cgatgtcgcc tgtaccacca
    17821 agaccctgcc ggacttcccc ggtctgtggg cccgcctgat cggacgcgaa gcgtgagccg
    17881 caggtcgcgg gaggcgatca ccgacgaccc gcacgccggt ttcgatcgcc ccggcaaacg
    17941 cacccggcca cgaaccaagg accgccccac ctacacggcg gccgtggaag cgatggtggt
    18001 caccgtcgat cgcggccgct tccgctgcct gctggacgac gacacgatcg tcaccaccac
    18061 gaaggcgcgc agcctgggac gcaccggcgt gatcgtcggc gaccgcgtcc gactggtcgg
    18121 cgacacgact ggggacgaag gcaccctcgc ccggatcgtt gaggtgcagg aacgcgagac
    18181 cgtactacgt cgtactgcgg acgacgacga tccgttcgaa cgacccatcg tggccaacgc
    18241 cgaccagctg gtgatcgtgg ccgcgctggc cgatccgccg ccgcgggtgg gcatgatcga
    18301 ccgcatcctg gtggccgcct atgacgccgg cctcgagccg ctgctggtgc tcaccaaggc
    18361 cgatctggcc gacccggaag ggctgatggc cacctaccgt ccgctcggcg taagcgtggt
    18421 tgcggtcgag cctggcgccg acctgagcac gctccgggaa gcgctggacg gccaccgcag
    18481 cgtgctggtc ggacactccg gagtcggcaa gtcgaccctg gtcaacgcgc tgatcccggg
    18541 tgtcgatcgg caggtcggcg aggtcaacga ggtcaccgga cgcggccggc acacgtccac
    18601 cagcgctgtg gccctgccgc tgccagaggg cgccggctgg gtgatcgaca cccccggcgt
    18661 tcgctcgttc gggctgagcc atgtgacgcc cgattcgatc atctcggcct tcccggacct
    18721 cgccgacatc gtcaccgact gcccgcgcgg ctgccgccac ctgtcgagcg acccggagtg
    18781 cgctttggac gtcgccatga gtgagggccg gctcgctgcg gagcggctgg agtccttccg
    18841 caggatggag aaggcgttcg gctagattcc gccacccacg ggcacggtgt ggcaggcgtt
    18901 ggctgcgggc gcaggcaact agattgtcgg ccatgcccga ccaatcgctg ctggacgacc
    18961 tccggctggc ccacgtcctg gccgaccagg cggattcgat cacaatggcc cggttcaagg
    19021 cgtccgacct gaaggtgacc gccaagcccg acctcaccga ggtgaccgac gccgacaccg
    19081 ccgtcgagga cgcgatccgc gccatgctgg gccgtacccg accacgcgac gcggtgcacg
    19141 gcgaggagcg ggacgactcc ggctggggtc cgcggcgttg ggtgatcgac ccgatcgacg
    19201 gcacgtccaa cttcgtccgt ggggtgccgg tctgggcaac gctgatcggg ctgatggttg
    19261 gtgatgaggt cgtagtgggg gtggtctcgg cccccgccct ctcgcggcgc tggtgggcgg
    19321 ccaagggcgg gggcgctttc accggccgat ccctgcttca gggcacgccg tgccgcacgt
    19381 ccgcggtggc cagcatggag gatgcgtcgc tgtcctactc gtcgatccac ggctgggtgg
    19441 aaacggggcg tggtcagcag ttcgtcgacc tgatgcggga ctgctggcgg acgcgcgcct
    19501 acggcgactt ctggagctac atgctggtcg ccgagggtgc ggtggacgtg gccgccgagc
    19561 ccgatctggc gctgcacgac atggccgcgc tggtgccgat cgtcaccgaa gccggtggac
    19621 gcttcaccaa cctggacgga tcagccggcc cgcacggccc cggcgcgatc gcgaccaacg
    19681 gaccgctgca cgacgagatg gtcgagcgcc tagcgctcta ggcgtctgga tcagtgccgc
    19741 tcaacgctgg ctgagcctgc cgaagcccct ggtgataccg cgtgtggacg acccttcgac
    19801 gagctcaggg atcgttgctc agagtgtctc tgtccagcgt gccctaccag agaccagttc
    19861 ccgggtaacc cgctcggcag cgagttccag gttccactcc cccgcggcca gcgcggccgg
    19921 cagatcggcc accgtaccgc tgatcgggac gtatgccagg acgtccgctg agctgagtcc
    19981 gggctcgact cggccggcga aaaccaccgt cggcacaccc acggcagcgg ccagcgcggc
    20041 caggccgtgc ggcactttgc ccgccagcga ctgggcgtcg acgctccctt ccccggtgaa
    20101 aacgtagtcg gcgccgacaa tgtgctcggc gagcccgatc gctcggctca ccagttccac
    20161 ccccggcacc agttcggcac cggtgaaagc caggaaggcg gcacccaagc caccggcggc
    20221 gcccgctccg gcgcgatcgc ggacgctcac gccaagactc gactcgacca cgtccgccca
    20281 acaggtcagg gcggcgtcca gttcggtcac agctgccgcg tccgcgccct tctgcggccc
    20341 atagaccacg ctggcaccac tcggcccgag cagcggattg gtcacgtcac tagccaattc
    20401 gatccgcact gcggccagcc gcggatcgag cccggcgcag tcaaccgacg ccagccgtgc
    20461 cagcgcctca cccccggacg gcagcggctc gccggccgag tcgaggaacc gcacacccag
    20521 ggcgctgaac aatcccgccc ccgcatcgtt ggtggcggtg ccgcccaacc cgaccagcag
    20581 ctcgctcgcg ccggcgtcca gcgcggcccg aagcaactca cccgcaccga aagaggacgc
    20641 ggtccgcacg tcccgtaggt gcgtcgggac cagctccaga ccggccccgg ccgcgacctc
    20701 gacaatggcc agccgacgat cggccaccca tccgaactcg cccacaatcg gccgacccaa
    20761 ggcgtcatgg cacggcaccg gcactgaaac gccttgcagc gcagccacca gagtgcggca
    20821 ggtgccctca ccgccgtccg ccatcggcac ctcgacgcag tccgcgtccg gcacggcccg
    20881 acgaatgccg cgagccattg cggcggccgc ctgcaccgcg gtcatcgact ccttgaacga
    20941 gtccggtacg cagacgatcc gcagcgacac ggctagccca ccttgatgcg tgggatcgcg
    21001 gccagcaggg ctctggtgta ctcctggcga ggctggctgt aaacggcccc ggtcgcaccc
    21061 gcctcgacga tccggccgcc gcgcatcacc gccagttggt cgcacagatg ccgcaccacc
    21121 aacaggtcgt ggctgaccac gatcagggtc agcccgcgcg aacgcaccag gtcggtcagc
    21181 aggttcagca cctgggcgcg aacggacacg tccagcgccg agaccggctc gtctgcgatc
    21241 agcacgtccg gacgcggcgc cagggcgcgg gcgatcgcga tccgctgccg ctggccgccg
    21301 gagaactcgt gcgggtaacg gtcggcggaa tcggccggca gatccacctc agcaagcacc
    21361 tcggccaacc gggcgcgatg gtccccgtcc gccaactcct tgcgcaccag ccgggaacgc
    21421 agcggctcgg tgatgatgtc acccaccttc atccgcgggt tcagcgacga gcgcggatcc
    21481 tggaaaacca tctgcaccgc agcccgcagg aagccgagcc tgcgttccgg cagcccggtg
    21541 atctcagtac cttcgaacca gatccggccc gccgtcggcc gatcgagccc ggccaacagt
    21601 cgcaccagcg tcgacttgcc cgagccggac tccccaacga tccccaggcg tcgaccgcgg
    21661 tccagcgtga ggtccaccga atccagggcg accacctccg cgccgccgtt gcggtagcgg
    21721 cgggtcaacc cttctgcgcg gaacaccggt tcggtcatgg ccgcctcgca tggaagtcct
    21781 cgaccgtcgg caggcgctcg cccggctcga tctggtcgag gcgcgcggtg gccaccaggc
    21841 cgcgggtata ggggtgctgc ggacggttga acaggtcggc gaccgtgccc tgttcgacga
    21901 tctcgccgtc cagcatcacc agcacgttgt ggcacacctg cgaaaccacg gccagatcgt
    21961 ggctgatgaa caggcaagcc gctgcggaag ccgtgagcac ctcgtccagc ttctccagga
    22021 cgcgagcctg cacggtcacg tcgagcgctg tggtcggctc gtcgcagatc accaggtcgg
    22081 gtgagttgat cagcgccatg gccatgatca cccgctgccg ctggccgccg gacagctggt
    22141 gcgggaaggc gtccgccacc cgctgcggat cggtcagccc gacctcgccc agcatggaaa
    22201 ccacccgctc gcgcgccgcg ccgggctcgg cgccctggtg cagccgcagc acctcggcca
    22261 cctgccggcc cacccgcatg gtcgggtcga gcgcggtcat cggctcctgg aagaccatcg
    22321 agatcacgtc gccgcgcagc ctggccatgc cgacttcgtc cagaccaacg atctcggtgg
    22381 cgttcaggct gatcgatccg gtcacggtgg cgttctccgg cagcagtccc atcaccgcca
    22441 gcgcggtcac ggtcttgccc gaacccgact cgccgatcac cccgagtcgc tcgcccgccg
    22501 ccaggtcgaa gctgatgccg cggacggccg gcgtccgact tcgcccaaag ctcaccgaga
    22561 ggtcgcgcac gtcgagaagc gtcatgacac ctcccgcagt cgtgggtcga ggtagtcgcg
    22621 taacccgtcc ccgagcaggt tgaagccgag cacggccagc gcgatcgcca ggccgggcca
    22681 gagcgcctgc aacggagcgg tgacgaggaa ctgttgcgaa tcgcgcagca tgttgcccca
    22741 ggtggggttc ggacgcggcg tcgccaggcc caggtaagac agcgcagctt cggcgaggat
    22801 tgccatcgcg aaacccaccg atgcctggac gccgatcagc ggcgcgatgt tgggcagaac
    22861 gtgccggccg gcgactcccc agcgggaggt gccggacgct cgcgcagcga gcacgaagtc
    22921 gctggccatc acctgtttcg cgcccgcgcg cgcgaccctc gcgaaagccg ggatggtggc
    22981 gacgccgatg gcgatcatcg cggttagcgt ggacgatccg tagaccgcgg ccagcagcgt
    23041 cgccagcagg atcgcgggaa aggcatagac gatgtcggcc gaacgcatca ccacctcgtc
    23101 gacccagccg cccagctggc cggcgagcat gcccagcggg acgccgacga tcgatgcgag
    23161 gccgaccgag atgatgccga cgaccagtac cgtccgggca ccgatcatca gccgggaagc
    23221 gatgtcgatg ccaaagccgt cggtgccgag cacatgcggc cagcccggcc ccagcagtcg
    23281 ctgggacggc accacccgga tcgggtcgaa cggggtccag aagaccgcga cgatggcggt
    23341 gatcaccacc agcgcaacca ggaccaggcc ggcgaccagc tgtgccttct tcacgaggcc
    23401 gcgcccccgt ccgcggaatc gacggcggct tggcgcgggt cgatcagcag gtaggagaag
    23461 tcgaccagag cgctgatcag cagtaccgcg agcaccagga acatcacggt gccctgcacc
    23521 accagcaggt cacgctgggc gacggcgctg agcagcatcg aacccagtcc gggcaggttg
    23581 aacacagact ccacgacgat cgccccgacc agcactgacg cgagctgcag ccccagcacg
    23641 gttaccagcg agatggctgc gttccggacg ccgtgccgca gcagcgcgcg catcggcgtc
    23701 cagccgaccg agcgcgcggt tcggtagtag tcctcgttca acacctcgat gaaggccgag
    23761 cggacgtagc ggatcaggat cgcaccctgc accgcagcca gggtgaccac cggcaggacc
    23821 agatgagcgg cccactggcc cggatccttg gtcaaggaca cgtagccgtt ggccggcaac
    23881 cagcgcagcc agaccgcgaa caccagcacc agcaggatgc cgccccagaa caccgggatc
    23941 gccattccca cctgtgcggc ggcgctgatc gcgaaaccgg gccaggagcg acgcttgagc
    24001 gcagcaacca tgcccgccgg cagcgcgatg atgagtgagc cgatcatgcc gaagccgacc
    24061 agccacgagg tcaccgcgat ccgtggggcc agcagggcaa gcaccggctg gccactgagg
    24121 tgcgagtggc cgaagtcgcc gaccagaatg ccgccgagcc agctcaggta ctgcacgatc
    24181 agcggccggt cgagcccaag ttcgcggcgc aactcggcga cggattcggg ggtggcgtcc
    24241 gtgccgagca gcacctgcgc gacgtcaccg ggcagcgcct tcgtgatcag gaagatcagc
    24301 agcgatgcac cgagcaggct gatcgcgaac acgccaaggc ggcgtccgat ccgccccgca
    24361 acgtgcatgc tctaacccgg ctagcggctc gtgatcgagg tgaggtcgaa gctcaggccg
    24421 ggtgcgttgg tggacacccc ggagatgttc gacttggtga tcaccaggtt gggcagggag
    24481 aacagccaga tcgcggcgca gtcgtcggcc aggtacttga ccgcctgctt gaagcccgcg
    24541 accgcctgct cgtcggacgc ggccgagtcg gcggcggcgt agagcgaagc gaactcggga
    24601 tcgccgtagc gccagtagta cttcgggtcg gcgaacttgc ccaggtcgcg gggctcgacg
    24661 tgcgcgacga tcgtcagatc gtagtcggcc ttggtgtaga cctcatcgat ccagcgggag
    24721 aactcgagct cctcgatggt ggccttcacc ccgatgtccc gcaactggct ggcgacgaac
    24781 tgggccgact tcaccgcgta aggaatgacc gggacgcgaa gccgcagggt gagcccggac
    24841 tcgtagccgg cctgcttgag caacgccttg gccttttccg ggtcatatgg gttggtaccg
    24901 gtgaggtcct cgtaccaggg gtcggtgggc acggccatcg agccgatcag ggcgccctgg
    24961 ccgttccaga cggtgtcgcg gagtgcctgg cggtcgatcg ccatggtcag cgcctgccgc
    25021 acttcgagct tggccagcgc cttgctctcg tggttcaggc ccagcaccac ttcaccgttg
    25081 gtggtgcccg acgtggtggt gaactggctg gtgtcggcga actgcgggag cgcgtccggg
    25141 gcctgcaggt tcgagatcac gtcctgatca ccgctggaga gcgcggcgtt ctcggcgttc
    25201 ggatcggtga aataggtgaa ggtgacctcg tcgaaccggg cgggattggc ccagtaggcg
    25261 gtgttggcgg cgaggatcac gttctggccg cggttctgcg ccttgaacac ataggggccg
    25321 gagccggcgg tgctggtggc cagatcaccg gtctcggccg ggtcgaggat catgccgagc
    25381 gtggacgaca tgtcgtagag ccacatgaac gacggcttgt tcagcttgat cacgaccttg
    25441 gtcgcgtcca gcgcttcggc ggacgcgacg acggccagct gcaggccgag cgcgtccacg
    25501 agcttggtgt cggccttgag ccgattgatg ttggcgacga cggcggccgc atccactgcg
    25561 gcaccggacg cgaacttggc cgccgggttg aggtgaaagg tgtaagtgag ccggtcgtcg
    25621 ctgatctccc aggcctgggc gagcagaggc ttgagcaagc cgctggagtc gaccttcacc
    25681 agggtctcgt agacgttata gagcagcacc tgcggaattg aggcggccgg gttgtgccac
    25741 ggatccatgg acgctggttc cagcgtggcg gcaattttga gagtgcgtgg agcctcgggc
    25801 gcgggggctg aggtgtcgcc ggtggtcgcg gtgccgctcg gtacggctgt cggggtacag
    25861 gcggagagaa ccatcatcga catggccagc atggccacga gtaccttgga aataccggtt
    25921 cgcgaacgtc gggactcagc ggcgacgtag gtcatctacc ctccacggga tctgtccccg
    25981 cggcgcgagg aagcgcaccg attctagcgg agcgccttcc gggcacctct gaacgcgttc
    26041 caccggcggt tcggccgcgt ccgcgctcgc cgttgcaccc ctcgatttgg gtatccagcg
    26101 ggtctgacct aagctggatt caccgacgcg gggtggagca gctcggtagc tcgctgggct
    26161 cataacccag aggtcatagg ttcaaatcct atccccgcta cgagtggaaa cccgctcccg
    26221 actagggctg atagccagat cgggggcggg tttctgcatg tctgagcaag actcaggtcg
    26281 cggccgattg gacacgacct tcgcctcacc gctgccgtcc ggcgctcaca gccaatcgag
    26341 tccccagcgc aggaactcgt cccgccgccc gcaaatctga cgccgacgtc gcctggctgt
    26401 ggctccgccc accgtcctgc gtggacgcag ccacagttaa cgctcgcagg cctcctgacc
    26461 cgctgaagcg ctcctcaacc gcgttcctca gggagcaggt tgcggagtgc ccccgtccga
    26521 ggtgtgggcg cacgtctgct catatgcgta cgtgttaggg agggatgagg attgatggcg
    26581 gaaagtctcg ccgcgatagt cctaatcctc tcctaccatc ggaggcacta tgcggatcac
    26641 ccgcatcatc atcgcgacag cagccatcgc caccggagtc agcctcgtcg ttgtggcccc
    26701 tgcggacgcc cgcggccgca gcccaacaag aaccccacca cgaccacctc cacggtgttg
    26761 accaccgcgg tcggtgcgcc gttcaacctg gacgtcacgc gcggcaaggt gctggttgcc
    26821 gacggcggcg gaccaggcgt cccgatcacg gccagcggcc tgacgatcac cggaccggac
    26881 ggcttcaagg taaccgctga caccctggcc cacgagaacg cgaacaaccc cgacggcaag
    26941 gtcttctacg gcgtccgcaa cccgagccag tgcgtgaccg acgagctcaa ccggatccaa
    27001 ttgccggtcg cccataccgg acacatcgac tcccacgcat actcggtggc cgcgtacggc
    27061 aagggcttcg tcgtggctga cgccggtgcg aacgcgctgc tgtgggtgag ccgctcgggc
    27121 aagatctcga ccctcgcggt cctaccggcc cagccggcca ggatcaccgc cgaaggcgcc
    27181 gcaactctcg ggctcgccga ctgcgtggtc ggggtcaagt accgcttcga agcggtgccg
    27241 accgatgtcg aggttggtcg tgacggttac ctctacgtca ccaccctgcc cggcggccct
    27301 gaggacccga gcctgggcgc ccgcggcaag gtgtatcggg tgaatccgtg gaacaggcgc
    27361 gtgcgagagg ttgcctccgg gctgctgggc gccaccaacc tggccatcgc ggacgggaag
    27421 atctacgtca ccgaactgtt cgcgggtcgc gtctccctgg tcaagcacgg ccgggtcagc
    27481 gagtacgtct cgctgccggg cgccctctcg atcgaggccg gtccgcgggg gaccctgtac
    27541 gccggcaccg gcatcgccgg gccgtcgtcc gtggtgaaga tctccacctc tggcaagggc
    27601 tggcggcact agccggatct agcagccgat ccggcggggc gtgcggcgac gcaccgccgg
    27661 atctgctgtg cttggcttgg ctgccggacc taccggacgc actgcgagat cccggcctgc
    27721 accgggatga cgccgtgacg gtgaccagga acgacgtggt caaaccgacc aggcaagggg
    27781 acatcgttgg cacaccgctc cggaagcgaa ctgctcgccc tgcacggcgt ccgggtactc
    27841 ggcggcccga gcgtcgccgc cgttgccgcg cggttccgac tcgccccgac cgacgttcgt
    27901 gagcacctat tggacgcgca ggcctacggc tgggtgaacc gtcacgagca ctacggcgaa
    27961 acctggtcgc tgaccgcgtc cgggaaggcc gaggacgagc gccgcctggg cgccgaactg
    28021 gacgccgtgg gctgccggga tgtggtcact gccgcgcatc gcgccttcct gccgctgaat
    28081 cgcaggcacg gtgaagcctg cacccgctgg cagttgcgtc cacagccctg ggacgccctc
    28141 gccgcgaacg accacaccga cctgcgctgg gaccacgccg tcctcaccga cctcgacgga
    28201 atcgatgctg ggctcaccca cggcctggaa ccggtggcca ccaaactgga gcgcttcggt
    28261 ggctatgccc agctacaccg tcaagcgctg gtgaaggtcc gcggcggcca cccggagtgg
    28321 ctggactccc ccgccgtcga gtcctgccag ctgatctgga tccagttgca cgaagacctg
    28381 ctcgctacgc tcgggattcc ccgcggcagc gacaccgtgg ccccagaatg aacccatgaa
    28441 gtccctgcag cagaacccat gccagacgaa gtgcgactgg tatccgaccg gcgaggtggt
    28501 ggccgaggac ctggtcttcg cctgcaccag ttgcggcagc gaatggaccc ggcaggaggg
    28561 ctggaagccg cgcaacctgg acgggtcagt ggccgccgag gttgagctcg agctcgctcg
    28621 ggacggctcg taactcgaca tcaccggcct gcggcgcact agtcactgtc tggggtgccg
    28681 accatactga cgccatggcc tacgaccagg aactcgccgg acgcatccgc gccgcgctct
    28741 cgttccagac cggcatcgtc gagaagtcga tgttcggcgg acgcgcgttc ctgaaggacg
    28801 gtcacctgct cgccagcgcc agcggtcgtg gcggcatgct gctgcgggtg cagccggaag
    28861 tcgcgtccgc cctggtggcg gaggggtcgg ccagcttctt catgatgcgc ggacgtgaac
    28921 tgcccggctg gttgcacgtc gagttggacc cggccgacga acgcctgacc gctttcctcg
    28981 acctgggtct cgccttcgtc gccaccttgg aaagcaaagc cgtgaaccgc ctgggacccg
    29041 accgcagccg gatgtgatga gatggagtac gtgcccgaat ccgtgatcgc ctatccgatc
    29101 cacacccgat ccctcccgaa cggactgcgg gtggtcgtca gccccgacca cagcgtgccg
    29161 gcggtcgcag tcaacctctg gtacgacgtg ggctcgcgga acgagcggcc cggcgaacgc
    29221 ggttgggccc acttgttcga gcacctgatg ttctccggct cggaacacgt cgccagcggc
    29281 gagcacctga atgcacttca ggcggtcggc ggcatcgtca acgccaccac ctggttcgac
    29341 cgcaccaact acttcgagac gctgcccgtc ggcgccctcg aactcgccct ctggatggag
    29401 gccgatcgcc tcgccaccct gcccgaccac ctcaccgagg ctgccgtcga cacgcagcgc
    29461 gaggtcgtca aggaggagaa gcggcagcgc tacgacaacg tgccctacgg cgacgccatg
    29521 gagcacctgg tcgaactggc tttcgggccc gagcatccgt acggccacac cacgatcggt
    29581 tcgatggccg acctcgacgg cgccaccgtg gccggggcgg cgagcttctt ccgcacgcac
    29641 taccggcccg acaacgccgt gcttaccctg gtcggcgaca tcacgccgaa ggaagcgttc
    29701 aagaaggccg aacgcgcttt cggaaaggtg ccgggcggag cggcccggac gttcggcgcg
    29761 ctgaactctt cccggcccag ctcgccagcc aacccgagtg tgccgcccct tgccggtaca
    29821 ccgacccgga aactgagcgg acgggtgccc gctgcggcca cctacttcgc ctggcgactc
    29881 cccccgcgcg acacccccgc cttcgacgcc tgcgacctgg cgctggcggc gctgggggcc
    29941 gggcagactt cacggctgca ccagagactc gtccgggagc agcaggtggc cgccggcgcc
    30001 ggcgcgtccg ccctgccgct gatcgcaggc aactccctcg gactggtgca ggtgcgctcg
    30061 cttcccggcg atggtggcgc ggctgcagcc gccgcggcgc tggccgaggt ggagcggctg
    30121 gccgaaaccg gtccgaccca agctgaactc gaccgcgcga agatccagtt cgagcgcgaa
    30181 tggctcaccc aactgtccag gttcgacgcc cgcgccgaca ccatcagtgc gttcgcggtg
    30241 ctccacggcg accccgaacg catcaaccgt cggctcgacg aggtgcgttc gatcaacctt
    30301 gacgatgtgg cccgggcgtg cgccacctat ctccaacccg cgcagcgtgc ccagctcgac
    30361 tacagccagg aggcagatca tgcggaaggc tgaccagcgt ccgccggtga aggccgcgcc
    30421 gctgtggcac ttccccgagc ccgccaagtt gtggctcgac aacggcttcg aagtgctcgc
    30481 caaccatcgg cccgggcagt acgtcgcgtc catcgccatc gtgctggacg caccgctgag
    30541 cgaggagccc gcggagatcg agggggtggc gaccatcacc cagcgctgcc tcgacgaggg
    30601 cacccgcact catcccggcg ccggcttcgc cgaagcgttg gaggacatcg gcgcgatcct
    30661 cgggggtagt gcgggctatt ctgccagcga gctgtcctgc gacgtgccgg tgagccgcct
    30721 gccgcaggcg ttgcgactac tcgccgaagc cgtgcgcgaa ccggagctgc ggcccgacga
    30781 catccagcgc caccagaccc tccggctggc tgagatcgag aacacgctgg ccaactcgac
    30841 ccagcgggcc caactggctt tccgcgcggc ctgcgtcccg ggacgcttcc gctcggcgcg
    30901 gatggccggt gggcaggctg ccacggtcag ccaggtcacc gtggacgatg tggccgcctt
    30961 ccatcggcgc tactaccgtc cggacggcac caccctgatc gtctccggcg acctgaccaa
    31021 ggcggtgttc cgcgaagcgg aagccgtctt cggtgattgg gacgtgccgg ccccgaacac
    31081 cgttcggcac cagtcgccgg tctcccggcg tccgcactgc tggctgatcg accgacccgg
    31141 tgccgtccag gcggacgtcc ggctgggcac tttcgggatc gaccgtggcg acccccgctg
    31201 ggcggacgtg caggtggcca cctatgcgct cggtggcgcc ttcctgagcc gactgaaccg
    31261 cgaactacgc gaggaactgg gctacaccta tggcgcgcac ctggtgaaca cgccgatgcg
    31321 cgatggcggc ctgatctcgg tacaggggtc gttccgtacc gaggtggttg cggacgcggt
    31381 cagtcgcgcg cgtgacctac tgaacgtggc caaccgcccg ttcaccagca ccgaggtggc
    31441 ggacgcggtc gcctacgcca acggggtgtc gccactgcgc tactccacag cgcagggcgt
    31501 gaccgatcgg atcgccaccc tggtcgcgga cggcgtgaac ccggatttcg tgaacaccaa
    31561 tgcgctggcc cttactcggg tcacccccga atcggcgacg caggcgatca ctgaactgct
    31621 gcctcccgac cagctgtcgc tcgtggtcgt cggggacgcc gctcagctcg ccgagccgtt
    31681 gcgcgacgcg ggctggacga tgtccgtcaa gccctgagcc ttggcccgcc gacgtctcga
    31741 caagatcgac cagcaggctc ctagcgctgg cccagtttct ccagggcggt ggcgaccgcc
    31801 tccttggctg cggtgacctg cttctggtac tcggcgagat caccgttgcg caacgctgcc
    31861 tcggccgcgg tgaaggcggt ctcggcagcc tgcatagcct cggtcgcggc cttctggtcg
    31921 gcgggcgtga cctcggtggg ctcagccggt gtggtcggct ccgtcgggtt gttcgggttt
    31981 tcgggcaccg ggttctcacc ggtgtccgca ccggcgtcgc cggagaacac ctggtcgagc
    32041 gcagcttgca gggtgctgcc gatgccgacg tgctcgccga aacgcaccgc gacgtactgc
    32101 agcgccgggt aggtgcccga gcccgatccg gactcccgga tcgtgtagat cggcatcacg
    32161 tagagcaggc cgctgcccat cggaagggtc agcaggttgc cgtactgggc ggccgccgaa
    32221 ccctggttca ggtagggccg catcacctcg gcgaacttct ggtcggtggt catcgcggtc
    32281 agggtctggc tgggaccggc gatctggtgg gtgtccgaca tccgcagcac gcgcatccgg
    32341 ccgtagtcgg ggctgctcgc ctcggccacc accgagatgt aggacgcgag gttcgcgcgt
    32401 ccgttgggca cgaacacgcc ggtctggctg aacactggcg acgcgtctcc gggccacttg
    32461 atcgacaggt agtagggcgg ctcagccgga ccggaccgag ccgtctcagc gctctggccg
    32521 ggggtcgtca gggtgggatc ggcaggcacc tgccagaggt cggactgctg gtaccacgag
    32581 ttcgggtcag tcatgtggta gcgcgtgagc aactggcgct gcaccttgaa caggtcctcg
    32641 gggtagcgca ggtgagccat caggtcggtg gagatgtccg ccttcgcctt gaccgtgcca
    32701 gggaacgcct tcatgtacga ctgcaggatc gggtcctcgg cctcccacgc gtacaggtcg
    32761 accgtgccgt tgctggcgtc cacgacggcc ttcaccgagt tgcgcaggta gttgacctgt
    32821 ccctcgacct gggtgccgac ggcgtcggac tgggaatcgg tcgtggccgc tcgcagtgag
    32881 gtgagctggc tgttcggata gttggcggtc gtcgtgtacg catccacgat ccagaccagg
    32941 cgtccgtcaa ccactgccgg gtagacgttg gaatccaggg tcagccaggg cgcgacctcg
    33001 gcgacacgct ccttgggcgt tcggttgtac aggatcttgg acgccgcgtt cacccggtcg
    33061 gacagcagga agttccagtc ggcgaagtgc gccgcgtaga ggacccgggt gaacaggtcg
    33121 ccgatcggca cgccgccggt accggcgtag acgttgttca cctcgccggc accctgaccg
    33181 ccgccaggac tgtcgaactc gatcggagcc tggccgggaa gccgtccgac gatcgcgaaa
    33241 gtggtgctct tctcgccgaa gtagatgcgt ccctcgtagt caggcagtac gccttcgggc
    33301 ggaaggttct tctcgatcca gactggctca ccggccgcac tgcgctggtt gccgtaggca
    33361 gcgaccatgc cgtagccgtg ggtgtaaacg gtgtgcaggt tgttccaggc ctggtcgggc
    33421 agcccggcga tgttgatctc ccgagccgcg acgacggcgt cggtctcctt gccgtcgatc
    33481 acgtagcggt ccacgtccag gacctcgggg aaggtgtagt agccacgcaa ctgctgctgg
    33541 ttctcgaagg tgcgcggcac cactgccggg tctatcagcc gaatgccggg cagcgcctca
    33601 gcgtcctcct tcagttgccc cttgctgacc tgggtgaccg cttcgtactc ctccacctcg
    33661 gtgtcggcga tgtcgtaggc ggtgcgggtc gcttcgaggt gcgcttggat gtacggccgc
    33721 tcgaagtccg gctcgttggg cttcacctgg aaaccctgca tgatcatcgg gtaggcgacg
    33781 ctgaggatca ggctggagac gagcagcaac accagggcgg tgatcgggag cgtccagcgc
    33841 ttcaggaatc cgttggcgaa gaacagcgcg gcgcacacga aggcgatgat cgccagcacc
    33901 agcttggcgt tgaatcggga gtgcgcgtcg gtgtactgga tgccggtgaa cagtgcgtcc
    33961 ggttgcagca gcaggccgta gcggtcgagc agggtctgca ggccataacc gaccagcagc
    34021 agcccaccga ggatcgagag gtggatccgg gcggcgttcc cgcgtccgct ctgcccacga
    34081 tcacgtccgc cgacaatccc gccgacggcg aagtgcacga ccgcagcagc gaccagcccg
    34141 aagaacagtg cgctcatgat gaaggccaac aggtcgcgca ggatcgggta ctcgaacacg
    34201 tagaagccga tgtccatccc gaagtacggg tcggtggtgc cgaacggagt gcggttccac
    34261 caggccaggt actccatcga ctggctgagc acggacgtgc cggcgatcaa cccgaggaac
    34321 accgaaggca ccaggatcgc cacccagatg ttcgcttcga gcaggtcacg gtagcggtcg
    34381 aggacggcgc tctgcccggt gcggcgggtc tcgggacgca gccggaaggc gatccacatg
    34441 ttcaggccga ccgagagcgc catcaccacg aagaagatgg cgaagagcag cacctgtgcc
    34501 agcagttggg tggtgaacac ctgcgggaac gccaccgaat cgaaccacag cttccgggtc
    34561 cacaactcgg cgaagatgat gaacccggtc accagcacgc cgaccaccgc gatcgtcggt
    34621 agcaggggac tccgccgcac ggcctgggac gccacgttca cccgatctcc taaagcttca
    34681 acaccagcgt tggtaccggt gttcagtcca atgtatgtgc cagtgccgaa gcgagcgctg
    34741 gaacgagatc ggcaccaccc aacagttcgc cttcggagtc atcggacggg cgcacgcggg
    34801 ccaccgaatg ccgccccccg tccctggtca ccccgaccac tacgcgcagg tcgattcgct
    34861 tggggtgggt ggcgaccagc tcggcggcag cctgcggatc atcgggcagg tcggcttcgg
    34921 cgtccgccgg aaggaaggaa cgttccaggg cgagcgcgca cccggagacc gtggtcggcc
    34981 aggcgatccg gctcagcgtc accgcgaggt cgtcgccggc gtggaactcc tcctgctcga
    35041 tggaggagag gccgtcggcg aagccgccct gcaggtgttc ggcgagactc ggctcggccg
    35101 cgatcaggtc ggcggtgggc accagggcga acagtcgcgc cggctggtcc cagccggatc
    35161 gcccgacatg gcgctccacc tcgatcaggg cagcggtcaa cgcttcggac atcagcaccc
    35221 cgctaccgag tcggcctcgg ccgggtcagc gagcttggcc agggagtcgg tcgcttcggc
    35281 cagcgtgtcc accggcagca gccggatcga cgggtcgggc gtgcccgcgt ccgcgcaatt
    35341 cgcccggggc agcacgaaga tcgtcgcgtg atcagtcacc gcggctgcga tcttctcctg
    35401 cacgccgccg atcgcgccga cgttgccttc ggagctaacc gtcccggtgc cggcgatcac
    35461 ccggccagcg gcgatgtcgg ccgaaaccag ccgatcactg atcgccagcg cgaacatcag
    35521 tccggcgctg gagccgccga ccgcagggtc gaccgcgaac tcgaccgtgg gcgcgtagct
    35581 gtaaccgacg tccatgttga tgcccacgat cggcttgtcc ggctcattgg tggtcgcccg
    35641 cgtcgtcacg gtgagcttga gcatcaggtc gttgcgcagc acggtgaaca gcaccggatc
    35701 tccagggtgc cgattgtcgt tgaggatcat gtcccgcaca tcctggacgg tatgggtcgc
    35761 ggtgccgtcc accgccttga tcaggtcgcc gggttcgagg atgccgacgg acgcaccgga
    35821 ggtgaagacc tcagcgaccc gcggccagct ggtcacttcg atgccggact gccgcaggcc
    35881 ggcgacgacc gcatcggact gcgattccac catgcgctgg gattcgacct ggctgatctc
    35941 agtcgcgtcc ttgccgggct ggtaaaccgc gtccttcggc aacacctcgc gcgcgggcag
    36001 ccagtaggcg aacaggactt ccgggaggct aagcgtcact ccgggggcgg tgaccgaaac
    36061 ggtggtcatg cgcagttcgc ccgaggtcgg gaaggtccgg gcacccgaga tcgtgatcgc
    36121 ttcgcgctcc cccacccgtc cgagcaggtc cgcggtcgaa cccggagccc aggtgacgta
    36181 cggcaccggg atgatcgcaa tcagggccgc gagcacgacg aacagcgcag ccgacaccgc
    36241 ggccgtccag gtctgcttgg tcaagatcgc cctctcgggt gctagtactt cgaggtccga
    36301 ggctacaccg gcgcctggag tccaagcttg gcaggtgttg gacaatggag gccacgagac
    36361 gaggagttgc gatggccgat gaggggtttg acaatcttcc cgagcgcggc ggcgaggagg
    36421 aggcgctgtt gcggttcctc gcccagttcg gcatcacccc cggaccggac ggtcgcctgg
    36481 acctcgaagg gctgatgacg cagttgcagc cgctgatgag caatctgacc gcacagctcg
    36541 ccagcttcgg gaaatcggat gccagcggca tgaactgggg cttcaccagg gatgtcgtgc
    36601 gcaaggtcgt cggcgcggcc ggagacgatc ccgagcccac gccggcgcag ctggccgcta
    36661 tccgggacgc ggtcgcgctc gccgacctct ggctcgatga ggagatcgcg ttcccgcggc
    36721 tgagcgcccc tgcgctcgcc tggcgtcgcg cggagtggag tgagcagacc tactcgacct
    36781 ggcagcggct ggtgcgtccg gtggtgaccc agctgtcact cgcactgcgc gggctgatga
    36841 cgggcggcac cggggagtcc aaggccgccg aaccgatgct gcggatggcc ctttccggca
    36901 tgttcgccgc gcaggtcggc caatcgctcg gcagcctggc caccaccgtg atcagctctg
    36961 gcgatatcgg cctgccactc accgaaactc cgcaggtggc gttgctgccc acgaacgtcg
    37021 ccgggttcgc ggaggggttg gaggctgatc ccgccgacgt cctgctgttc ctcgcggtcc
    37081 gcgagaccgc caggcaacgg ctgttcggcg cggtcggttg gctcggcccg cagatgctgg
    37141 cactggtcga gcactacgcc agggatatcg agatcgatcc ggaggcactg gaacaggcga
    37201 tcgagagcca actgaacgcg gctatgaccg caggtgaact ggagcaggcg ggcagcgccc
    37261 tggcgggctc gctgttcgcg ccacgggtca cggacgagca gcgccaagtg cttgatcgcc
    37321 tggagacgct gctggcgctg gtcgagggct gggtggatga ggtggtggcc caagtggccg
    37381 gccagcggat gcccgccgcg gcagccctga ccgagacgct gcggcggcga cgagcggcgg
    37441 gaggcccggc cgagtccgcc ctgaaagccc tggtcggact ggagatgcgg ccacgacgct
    37501 cgcgggacgc cgccaacctc tgggcggcca cccgagccgc tcgcgggggc gagtccaggg
    37561 acgccacctg ggaccatccc gatctggtgc ccaccgccga tgacctcgcc gacccgctgg
    37621 agttcgccgc acaggggcac cggacggccg cgcccgacga cctggacgcc gagctcgcca
    37681 agcttctgag cgaggagggc cgcggttaga gcgtgtctcc ctaaccgtca cgcgcggcca
    37741 gggccggcga ctagtcgacc tcgtcagcgc ccaaccagtc ctcgtcgccg ccatcctggg
    37801 cgtgctgata gccggcgagg aaggccttcg cctcggccgc cttcgggtag ccggcctcca
    37861 gtcgccggaa ggctacggag tggttcggtt cgaacaggtg cgcgagttcg tggatcagca
    37921 cgtagtccga gacccacgtc ggcatcgtgc gcaatcgcgc ggacagccgg atcgcaccgg
    37981 tggaactcgt gcacgagccc caacgccgct gctggttggt cgaccacttg acggtgaacc
    38041 ccggcagggg cgcggagacc cgctcggcga gataccgctg gaacaggtcg accgcccgcg
    38101 cgctcagttc gtcggcgagc accggtgcga gccgtctcga ctccttcacc aggaagcgtt
    38161 ccaccaacgg cggcagcagt tcgcgctcct gagcggcggt catgcgcgcg ggcaccaggg
    38221 ccaccaaccg gccgcgctcc cggaacaccg tgagcgtccg cgtccggcga gcgctgcgcc
    38281 gcacctccaa ctcggattcc gccaccatgt cccgcagatt acgacacgcc gccgacactt
    38341 ccagttgacc gtgggcgggg cgagcactag cttctagacg cgaggcctcc agtggccgct
    38401 ccgctcgcag gggaaggcaa gcggagtcgc ggtcgctgga ggccctcgca cgcaagcggg
    38461 cgctaccgtg tcgtaagcgt tgcccaagac atagactgac tcgcaattcg ctgctgagcc
    38521 ccagccgggg gtgacgctga tcaacaggag gaacaacgtg gccaaggaca cctacagcgg
    38581 ttcgttctac tgcgtgaagt gcaaggatca ccgggaagcc accggcgatg tcgtggtcaa
    38641 cgagaagggg acccggatcg ccaaggcgaa gtgcccggtc tgcggcacca acctcactcg
    38701 cttcctgccc aaggcctgag taaccacagt catagcgccg gccattcggc cggcgctttg
    38761 ctgttcccaa agccagccaa ctctggccaa accccgcccg ggatctgcgg cgacgtcgcc
    38821 cgccaatccg gcaactttgc tcccgaatgg gactctcgct cccaaatatt cgggagcacg
    38881 agtcccattc gggagcaaag tcggaaaccg accggctcgg ggggagtatc tctggatggg
    38941 tggaacccgg ctgcgcgagg tgttgtcaat tccgtccagg tgttgtcaat gtcgctgtgg
    39001 ctctcgcgcc gggccatcac cgcgggcacc atgagcgcat gctccgagtc agattgcggt
    39061 ccccgatcac gtcgctgtgg cgggcacccg gcgtgctcca gatcgggttg gacgcgccgg
    39121 cggtggtgct ggacggcgtc ccggcgcagc tcagggacgc cctgcgcctg ctgaccagcc
    39181 ccaagagcgt cgctgagctc gcggcgctgc tgcccaagct ggacgcccgc tggctcggct
    39241 ggctggtggc tcggctcgat gacgccggcc tgctggcatt cgaatcagca gcgccgcccg
    39301 agcccagggt gaccgtggtg ggtgctggac ccctcgccga agcggtacgg gcagccctgg
    39361 ccagccccga cgtgtcggtt ggtggtctgg accacgccgc ctatttcggc ggcagtctgg
    39421 aggcggacgg cacacccgac gacgtgctga ccgtcgttgc cgcagccaac tccgagcctg
    39481 accgaacgct caccgatgcg ctcttccgct ccggccgggc gcacctggtg gttcggctgg
    39541 agtccgaccg cgcggtggtg ggtccactgg tggtgccggg gcgcactccc tgcgtccgct
    39601 gcctcgacct gacccgctgc cacctcgatg ccgactggcc gcggctgttg gcccagctct
    39661 gcctcgaagc caccgaaccc gagccgtcgc tggtcacctg ggcagcggcc accgccatgg
    39721 ctcaggtgcg cgcctggacg gcggggcgga tgcccgagac cggcggcggc tcgctggaac
    39781 tcgccctgcc caactacctg ctgaccgcgc gacgctggcc ggcccacccc cgctgcggct
    39841 gtctgcagcc gctgggttga tccggtcggg cagactggcc gcgtgaccag ccaagacgag
    39901 gagcgccgcg tacttccgga gtcgaccttg acccgctcgg ccaagctgct gacgcttccg
    39961 ctgggcgccg ccgcgcgcgc cggcgtcggc atgggccgca agctgctcgg ggcccctgct
    40021 gaagacgtgg acgccgccat gcgggacgct gcggctgagc agttgttcca ggtgcttggt
    40081 gagctcaagg gtggcgcgat gaagttcggg caggcgctca gcttgttcga agcgatgctg
    40141 cccgaggatc tggccggccc gttccgcgag cggctgcgca agttgcagga cgctgccccg
    40201 ccgatgccat ccgcccgtgc ccagggggtg ctgcgggccg aactcgggcc gcgctggcgc
    40261 gaccactttg ccgaactgaa cctgcgtccg gcggctgcgg cctcgatcgg ccaggtacac
    40321 cgcggccggc tcgccgacgg acgcgaggtg gcggtcaaga tccagtaccc cggagccgac
    40381 accgcactgg cgagcgacct gcggcagatc cagcggctgg ctggtgcggt ctcgccgctc
    40441 accggcggcc tggacgtcgt cgcgctcgtc cgcgaagtcg ccgcgcgggt gaccgaggag
    40501 gtcgactaca ccctcgaggg cgtttcccag cagcaggtgg ccaccgcatt ggccgggcac
    40561 ccgcgcttcc tggtgccgaa cgtgcacctg gccacccggc gggtactggt ctcggactgg
    40621 gtggacggcg aaaagctgac cgcgatcatc gaccgtccac aagcggaccg gaaccgggtg
    40681 gccctcgact acgtcacctt cctgttcgcc gggccatcgc tggctggcat cctgcacggc
    40741 gatccgcacc ccggcaactt cctggtgatg ccggacggac gcctcagcgt ggtcgacttc
    40801 ggcctggtct cgcgactgcc ggacgggctg ccggaggcga tgggtcggct gatccggcac
    40861 gccgtcgacg gtgccgtgga gccgatggtc gccgggctgg ctgaggaggg cttcgtcgac
    40921 gactcgatgg acggccgcgc gctgctcgac tacctggcac cgttcgtcga gccggccagc
    40981 gtcgaggatt tcgcgttcaa ccgggagtgg gcgcgcagcc agttcgcgcg cgtccacgac
    41041 gacatgggtt cgtccggtgt ggccacgcgc ctgaacattc caccggagta ctcgcttatc
    41101 taccgggtgt ggatgggtgg catcgcggtg ctttcccagc tcgacgtgca cgccaatttc
    41161 gcccaggtgc tgcgggacta cctgcccggg ttcgctgact gaccgttcac cacccaccgt
    41221 catcggcgaa cctgcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    41281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncctgc
    41341 cccaccgtca tcggggagcc tgccccaccg tcatcgggga gcctgcccca ccgtcatccc
    41401 ggcgcaggcc gggatctctc ggaaccgtcg atggtgtgtg ctcagcggtc gttgcgacta
    41461 caacggcttg accgcagtga gcgtgaacga ggccggcagc cgctctggac gctcggccag
    41521 gcgatactcg tcgacttccg gatcgagttc catcaggccg ggcagcgcct cccatggcac
    41581 gctctggtgc tcggtcaggc cggtgagggt cagcccggct tcgagaacag ctgtgacgat
    41641 ctcgccgagg ctgtgattcc actccatcga ctccggcgaa ccgaccaggt cctcgccggc
    41701 ataggtgtgc tcgtcatgcc agaccagggc ttcggcacgc tcgtagtagg ggaactccag
    41761 cgcaaccgta cgcgccccgg cagccgtcat ccacggctgc tgcacacggt catccgggtc
    41821 ggcgccgagc acgaccggcg ccagcgcgag cagcactggg tgcccgtccc ggatgaagag
    41881 ccggccgccc ggccgcagca ggtcggccac cgtctgcgcc cagcgctgga tgctgggcag
    41941 ccagcagatc gcgccgatgc cggtgtagac caggtcgaag tcgcgaccag ccagagcttc
    42001 cggcgccgaa tagacgtccg cctcgatgta gtcgatgtcc gcgtccgccg ccgcggcaag
    42061 tcgccgcgcc tgctccagcg agaccggcga aaggtcgact ccggtcacct gggcgcccag
    42121 acgggagaga ctgagcgtgt cagtgccgat gtggcactgg aggtgcacga catcgagccc
    42181 cgcgagattg cccagacgcg gcaggtcgaa gcgcaccaca gctgaaagtg cggccggatc
    42241 ggcgagcagc ccttcgatgc cgtaagccgc ggcgtggatc ggggctcggc tgtcccagtt
    42301 ggcctggttg accgaaaggt agtggtcgct catgtctgct cctggtgctc ggcgacttct
    42361 tcgctgagga tggccagcac ggcctcggcg tactgttcgc atttcacctt gcccaccccg
    42421 ggaatggcga gcaactcgga cgcggttcgc ggtcggctct ccgcgatcac catcagggtc
    42481 gcgtcggtga acaccacaaa cgcgggctgg gccctcatcc gggcaacgtc cgcacgccag
    42541 gccaccaacc gggcgtaggt ctcctcgtcg tagccggcgg ggcaggccgc gtgccgcctg
    42601 agctttctct cggcgccggt ggtcagcggc ttgccgcaca cctgacaggc cgcgcccata
    42661 gccgagaccg gcttcgggcc acgacggacg cggaccggtc cggtcgacgg ccgagcggcc
    42721 aacccgtcca ggaagcggga cggctggcga aagccgcccc caccgccgcg ccgggcgcgt
    42781 gaccaggaga cgtggagcgc gcgcttggcc ctggtgatgc ccacgtagaa cagccgggcc
    42841 tcttccgcca cctgctccgg gccagccgcc agcgcgaacg ggagggtgcc ctcctgcaca
    42901 ccgaccaggg ccactgccgt ccattccagg cccttcgcgg cgtgcagcgt ggacagggtg
    42961 accccctccg cagcgagcgg atgctcggcc tgggcgcgtt cggccagctc ggccacgaac
    43021 tcaccgatgg tcgctccccc gccaccaccg gcgtccgcga ccgccgtatt cacgtcctcg
    43081 gccagggcga ggatcgcggc cagcgactcc cagcgttccc ggacgcggcc ctgcccggcc
    43141 ggcggcgtcc ccgtccagcc caaccgggca agcagggctt cgcaggcact gatcgcgggc
    43201 acggacggat cgcgcctggc ctgctcggcg agcgtcccga tcgcctggcg aacttcgggc
    43261 cgctcgtaga agccttcgcc gccgcggacg gtgaacggca cctcggcttc ggtcagggcg
    43321 gcctcgaaca gtggagattg ggcgtgcacc cggtagagca acgcctggtc gcgccaaggc
    43381 acgccggcct cgtgctgctc gatcagccac ctggcgaccg cggtggcttc gtcgtgctcg
    43441 ctggcgcacg gcaggatgcg cggctgcgga cccggagggc actgcgactc gagcgtgacc
    43501 gtgcccagcc gggtgttgcg ggccatccgg ttggccagct cgaccacctg cggcgacgac
    43561 cggtagtcgc gaaccagccg cacgacgtgc gcttcgggat accgactcgc gaacccggtg
    43621 aggtagccgg ggctcgcgcc ggcgaaggag tggatggtct gcgccggatc gcccaccaca
    43681 cagtggtcac ggccctcccc ccaccacagg tcgagcaggg tctgctgcac cgggttcacg
    43741 tcctggtact catccaccac caggtggcgg taggtgccac gcacctcgtc ggcgatgtcg
    43801 tcgaactcac tcagcagcgc ggtggcgcac agcaggatgt cgtcgaagtc gatcaccccg
    43861 cgatcgcgtt tgacctgctc ataacggccc agaacgcgtc cgacctgggc ggggtcgagc
    43921 ccggagactt cgcggccctg cgctgtggcc agatcggcgt agtcggcggc cgggacattg
    43981 gtgaccttgg cccaggagat ctcggtgagc agatcacgaa gggtcgccgc atcgggcgac
    44041 agccgcagct tgcgggcagc tgcggccgcg atcggcaggc gctccgcggt cacctccggc
    44101 agggccgagc cgtacgcgcg cggccagaag tactgcgcct ggcgcagggc cgccgaatgg
    44161 aaggtgcgcg cttgaacggc gggcacgccc agcccgcgca acctggaccg cagctccccg
    44221 gccgcgcggg tggtgaaggt gaccgccagg acggcattgg ggttgaaggc accgatggcg
    44281 gacgcgtagg cgatccgatg ggtgagcgcg cgagtcttgc cggtgccggc tccggcgatc
    44341 accaccaccg gctgcccgaa cgcggtggcc acctggcgct gctcggggtc gagccccacg
    44401 agcagtgcgg tcgggtcggt gatcaccttg cacaccttag gctcgggcac ccacaaatac
    44461 tgtcggtggg ggcctctagg gtgagaccac catgcaagta cagcacgcac gcaccgccga
    44521 agacctggtc tcgcggctca cgtcggcctg gtcggggccg cggcccgacc cgttcgcgtt
    44581 cgatctcgcc gtggtgcccg gtgccggctt ccagcgttgg ctgtcccagc aattggcgtt
    44641 gtccgacgcg ggaatctgcg cgggcgtcga gttcgtgtcg ctgccggcct tcgagcgtcg
    44701 gctgggcgga accgacgatc cctggcaacc cgatcggctg gtctggcggg tgcagcggct
    44761 ggccctggaa cccgacctgc ccgaactgga gacccttcgc cggcacctgc tcgcctcccg
    44821 cgagagctac accgcctgca accggatcgc gcggcacttc gccggctacg cgcgccaccg
    44881 tccgaagttg ctggcggcat ggacgagggg tgaggacgcg gcgcccgacg ggcaaccgtt
    44941 gggcgacgac gcctggcagg cacagctctg gcggcggctg gtggccgaga tcggcgaaag
    45001 cccgctggac cgcaggctgg ccctgctgaa ccggctacgc agcggcccca ctcccgggct
    45061 gcccgaacgg atcgcggtga tcgtgccaac acagttccac gccgggctgc tcgaattgct
    45121 cgaagccgtg ggttcccatc accgggtcga gctgctggcc ctctgcccga cgccctcgcg
    45181 gcgcccggcc cgggttaccg ccgagcttcg tcgggccgac ttcacccggc cggtcggaca
    45241 tcggctgaac ctctcgctcg ggcagctcgg cgacgaggcg gctgccctgt tcccccccgt
    45301 cacgtccacg actgcgcctg agtcgatccc cagcctgctt ggctggctgc aggcgaatct
    45361 gcgcgacgac accgaagcgg cgccgcgggc gctggatccg tccgatgatt cggtgcaggt
    45421 gcacctgtcg cacgacctca gccgacaagt ggaggtgctc cgggaagtac tcacgtcggt
    45481 actggccgcc gaccccagcc tggagccgcg cgacatcgcc gtgatcactc ccgatgtgga
    45541 cgcggtcgcg ccgctgatcg gggcggcgtt caccgtggcc ggcggcccat cgcatcccgc
    45601 gacgcggttc cgggtgcagc tggccgatcg gtcggtcgct caggtgaacc cgatggccac
    45661 gctgctgcta cggctgctcg cgctgccgga tggccgcttc gaggcgtcca ccctgctgga
    45721 gctgtgccag gagccggcga tcgcggcgcg cttcgggttt gggccggacg cccgtgagcg
    45781 gctggtccgg ctggtcgaga ccgcaggcat ccgctggggg ctttcggcgc agcagcgcgc
    45841 cggtttcggg ctcggcgggt tcccccacaa cacctggttc gccgggctgc agcgcatgct
    45901 gctcggggtt gccctgcccg agaccgatct ggtcgccgcc ggcacggtgc tgccgctgga
    45961 cgacgtggaa tcttccgatg tcgcgctgat cggcggtctc accgagttcg tcggccggct
    46021 gtcgcgcgct ttgaacgagc tgtctgcgac cgggacgctt gccgagtggt gcgcccgcag
    46081 ccgaagcctg ctggagtcgg tggtctccct tccgcacggt caggagtggc agctgagcga
    46141 cctctggacg ggcctttccc ggctggcgga gcgaggtgag cagcctggcg cgccgcttac
    46201 tcgcgccgct gccgagcgcg ccatcgcgga cgagttcgcc ggggcgcccg cccgcggagc
    46261 gttcggcaat ggttcgctga tcgtctgcgg gcccaactca ctacggcacg tgccgcaccg
    46321 ggtgttggtg ctgctcggtt gggacgccga ccgctacccc aggccggtcc gccggcatgg
    46381 cgacgacctg ctcgggcagg agccgcgcgt cggcgatccg tccggcgcgc tgctggatcg
    46441 gcagttgctg ttggacgccg tgcacgcggc ccgccagcag ttgatcgtgg tcgcgcaggg
    46501 ccgcagcgtc gccaccaacg aagtggtccc gctggccgcc ccaatcgcgg agctggtggc
    46561 cgcgctcgat gcgaccgccg taactgcggc cgggctgggt gctggtgcag ccgtcacggt
    46621 ccagcatcca ctgcagtcgt tcgatgcgcg ctacttcggt ggcgacgcgc gcctgtacag
    46681 cgccgacccg ctcgctttcc gggcggcccg cgccagcgtc gccgagccgg aggccccgcg
    46741 tccgcggtac tacctggaga cgctgccgcc acccgacctc agccaggggg tgactctcga
    46801 acaggtcacc gacttcttca agcatcctgc gcgcgcgctc ttgaaggtgc gggccggcct
    46861 atcgctgggc aattcggcgg agtccagcga cgagttgccc atcgaactcg accacctgga
    46921 gcgctggcag atcggcaacc gggtgctgca gcggctgcgg gcgggccacg acgcggacgc
    46981 cgtccggcga gccgaatggc tgcgcggtga ggtgccgccg ttcgagctgg gtcggca
//
LOCUS       DAOPRQ010000006        46447 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_583_length_46447_cov_11.647676, whole genome shotgun sequence.
ACCESSION   DAOPRQ010000006 DAOPRQ010000000
VERSION     DAOPRQ010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 46447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 46447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46447
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_583_length_46447_cov_11.647676"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1772)
                     /gene="dxs"
                     /locus_tag="PKJ61_01260"
     CDS             complement(<1..1772)
                     /gene="dxs"
                     /locus_tag="PKJ61_01260"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HOC12493.1"
                     /translation="MSLLDEINGPEALSDLTPEQLQQLAEEIRAFLITNVSRTGGHLG
                     PNLGVVELTIALHRIFDSPKDAIVFDTGHQSYVHKVLTGRKDQFGSLRQPGGLSGYPS
                     RAESPHDWVENSHASTALSWADGLAKAFRLRGDDRFVVAVVGDGALTGGMVWEALNNI
                     AVSGDERLVIVVNDNGRSYTPTVGGIATQLSSLGKQLAAIRTDRRYERTLKRIKQLAR
                     KTPLVGEMVYGLLHGFKMGVKDVLAPQGMFSDLGIKYLGPINGHDIEAVELALAQAKG
                     FGGPVLVHCITQKGRGFKAAEEHEQDRFHAVGQIDEVTGEPLTDTPARTWTDAFGDEL
                     ARIGAIDESVVAITAAMLHPTGLDKFARHFPERTFDVGIAEQHAVTAAAGLAAGGMHP
                     VFAVYATFLNRAFDQVLMDVALHRQGVTFVLDRAGVTGPDGPSHNGMWDASLVGLVPG
                     LRLTSPRDEQRLGEALRLAVTVSDAPTVLRYSKERLPEPIPAVQQRGDVDVLFASQRP
                     RVLVVGYGQLCDTAVDVGRRLAQQGIGATVVDPVWALPVSPDLLALMAAHELVVTIED
                     NGVVGGMGARLGQEARALGIETRLR"
     gene            complement(1787..2032)
                     /locus_tag="PKJ61_01265"
     CDS             complement(1787..2032)
                     /locus_tag="PKJ61_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC12494.1"
                     /translation="MLRRHPVVLGMFAVEFVDSQARASVEGLARVRTGLADYVRPEVA
                     QEAVEAWTTEGARLVRLRREVGMVVEALRGRVFTPKL"
     gene            complement(2258..2734)
                     /locus_tag="PKJ61_01270"
     CDS             complement(2258..2734)
                     /locus_tag="PKJ61_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC12495.1"
                     /translation="MEPDFMPVSARVAWTYVAALIAVVGAALLVVVGGQVVSGTVCKV
                     VVDDSPDDFAISCLFWWGIWLAIGGFLVALVAAVRALKLDWWLALTLLAGAGLLVAVD
                     AITAWWWWVLAALLPALAALASADWDRGPRFRRTQQVALLVLAGLSLAAVIWWYLG"
     gene            complement(2770..5571)
                     /locus_tag="PKJ61_01275"
     CDS             complement(2770..5571)
                     /locus_tag="PKJ61_01275"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HOC12496.1"
                     /translation="MSIDSYSAADQLTVAGKTYQIFRVDAVEGSATLPFSLKVLLENL
                     LRTEDGANITATDIAALAGWDPSAEPNKEIQFTPARVIMQDFTGVPCVVDLATMREAI
                     AELGGDPAKVNPLSPAEMVIDHSVVADLFGTPDAFTRNVELEYQRNRERYQFLRWGQT
                     AFDDFVVVPPGTGIVHQVNIEHLARVVFPRVVDGVLQAYPDTCVGTDSHTTMVNGLGV
                     VGWGVGGIEAEAAMLGQPVSMLIPRVVGFKLSGKLPEGTTATDLVLTITEMLRQHKVV
                     GKFVEFYGDGVAEVPLANRATIGNMSPEYGSTIAIFPIDDKTVDYLRLTGRSAEQLEL
                     VEAYAKAQGLWHDPASEPRFSEYLELDLATVVPSIAGPKRPQDRVVLAHAKEGFNEAL
                     RDYVGDDISGYDEAVAESFPASDAPAHDGGNGAPAPHEYGEHTPAGLGRPSKPTPVTL
                     ADGTSFTLDHGAVTIAAITSCTNTSNPSVMIGAALVAKKAVEAGLERKPWVKTSLAPG
                     SKVVTDYYEKSGLTKYLNALDFDLVGYGCTTCIGNSGPLIPEVSQAVNDADLAVTSVL
                     SGNRNFEGRINPDVKMNYLASPPLVVAYALAGRMDIDLTTEPIGTGSDGNPVYLADIW
                     PSQAEVDEVVGSSITAEMFTSRYADVFAGDETWRSLPTPTGDVFSWDADSTYVRKPPY
                     FDNMPAEPVAVTDVAGARVLLKLGDSVTTDHISPAGSIKADSPAGRYLSEHGVERKDF
                     NSYGSRRGNHEVMIRGTFANIRLRNQIAPGTEGGFTRDFTVADAPVATVFDAAQNYAS
                     AGVPLVVLAGKEYGSGSSRDWAAKGTALLGVKVVIAESYERIHRSNLIGMGVLPLQFP
                     EGENADSLGLTGTETFSVTGVTELNDGTTPATVRVEAVGDGEPIVFDAVVRIDTPGEA
                     DYYRHGGIMQYVLRSLLNK"
     gene            complement(5654..5842)
                     /locus_tag="PKJ61_01280"
     CDS             complement(5654..5842)
                     /locus_tag="PKJ61_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC12497.1"
                     /translation="MNTPGTHPKRTRPEAKCPLRPEEICNLCQLNVTGPQDCGLVYLV
                     MSDPDQRLEWGERRRKPH"
     gene            complement(6161..7312)
                     /locus_tag="PKJ61_01285"
     CDS             complement(6161..7312)
                     /locus_tag="PKJ61_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012921370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HOC12498.1"
                     /translation="MTEPFAVDDVLAEVEIGPVAHGGHFVARVDGRVIFVRHALSGEV
                     VDVRITEAGKRFSRGEVVAVHRASPERVSPPCAIAGRCGGCDFQHVRPEHTRELKRQV
                     VAELLGHLAGYQFIGDVVEVPPAPFGWRRRMRYHLDAVGRPGLLAHRSDAVVPLPEAG
                     CRIAAPPIAHPPADPGRPGTELLAVAAASGVRFCGVERSAETVTEQVDGRSYEVALTG
                     FWQAHAAAPAVLTERVLDGLQPRAGETALDLYCGVGLFARALADAGAQVWGIEGDRRA
                     IELARTNVPSARFLVGDVARRLERLPNRADIVVLDPPRAGAGRAVLASVVARRPRRIA
                     YVACDPAALGRDLQIAADLGYRTMSVDAYDLFPMTHHVECVAILVPAST"
     gene            complement(7309..9351)
                     /locus_tag="PKJ61_01290"
     CDS             complement(7309..9351)
                     /locus_tag="PKJ61_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015070348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HOC12499.1"
                     /translation="MNAADSVKRVLVGRKLANAQLGETLLPKRIALPVFASDALSSVA
                     YAPDEILLTLSLAGMAGFAFSPWIALAVGLVMLIVVLSYRQTVHAYPSGGGDYEVATV
                     NMGPNAGLVVASALLVDYVLTVAVSVSSGVMNAKAMFPFLEGHEGLWAAGLIVLLMSM
                     NLRGVRESGSFFAIPTYGFMVGVLGMTIWGLFRIFGMGEHLQAPTANYEVIGAPAFEP
                     FAGLAMVVLLARAFSSGCAALTGVEAISNGVPAFREPKSRNAASTLALLGGIAITMLA
                     GIIALSNLTGLKLIDPDGASHFAQNGVPLEVAHETAMGQLARTIFDTFPAGFYFVITM
                     TMIILFLAANTAFNGFPVLGSILARDGFLPRALHTRGDRLAYSNGILALATAAIALVL
                     IYNASVTALIQLYVVGVFVSFTVSQFGMMRHWTRHLKTEKDPKVRATMQRSRAINAVG
                     LTGTGIVLVIVLLSKFIYGAYLAVLAMALVFVVMRAIRNHYDRVARETAVSPMDARLL
                     PSRVRAVIMVQEVNKPTMRAVNFARATRASSIEAVTVSIDDAETRSIVSQWQAQELEV
                     PLKVIASPYREVTGPFLNYVKGVRSDNPRDVVCVYIPEYIVGHWWEQFLHNQTGLILK
                     TRLNFMQGVMVTSVPYQLASSKYAIDRAKRNTAAAWRRPGSGQVRVGDSRGPELPE"
     gene            9489..10136
                     /locus_tag="PKJ61_01295"
     CDS             9489..10136
                     /locus_tag="PKJ61_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007433810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HOC12500.1"
                     /translation="MGCGRVGASLARSLERRGHSVAVIDIVQDAFRRLGPEFQGKTVK
                     GVGFDRQVLVRAGIERADGFAAVSSGDNSNILAARVVRETFEVHNVVARIYDQGRAEV
                     YERLGIPSVATVRWAADQVLRKLLPSGSEPQWRDPSGSVRLIEVHANSDWVGTKISAI
                     EAATRAKIPFLMRFGTGIVPNSATVFQDGDLIYAAVTNEQVPDVEDVLADPPVQH"
     gene            10151..10831
                     /locus_tag="PKJ61_01300"
     CDS             10151..10831
                     /locus_tag="PKJ61_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HOC12501.1"
                     /translation="MRVAIAGAGNVGRSIARELIANGHQILLIDRDPNAIKPESVPEA
                     EWLLADACELTSLEEASLDSCDVSIAATGDDKANLVHSLLAKTEFGVPRTVGRVNHPS
                     NEWMFDDLWGVDVAVSTPRLMCALVEEAVTVGDLVRLFSFRGGHANLVEMTLPDGSPR
                     VGVRVADLNLPGDAVLVTVIRDGSARAPERDAALEANDELLFVCDPANEAELAQYLVP
                     RARVTEAG"
     gene            complement(10957..11322)
                     /locus_tag="PKJ61_01305"
     CDS             complement(10957..11322)
                     /locus_tag="PKJ61_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HOC12502.1"
                     /translation="MSPNKFLSALRRLGATNAELEAQERQRVAEEVGAGRIGDLQDRQ
                     LARLRGTITVLAMKPRNGTPWLEAEFSDGSGTLTLIWMGRREIPGIVAGAQLAVSGRV
                     SYVDGERRLYNPYYELIAN"
     gene            complement(11322..12086)
                     /locus_tag="PKJ61_01310"
     CDS             complement(11322..12086)
                     /locus_tag="PKJ61_01310"
                     /inference="COORDINATES: protein motif:HMM:NF023917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3710 domain-containing protein"
                     /protein_id="HOC12503.1"
                     /translation="MIFGRKRKPADIDPVDTEEEALEIVGEDADDPADDEVEATETDE
                     LEDDDIESGDEDFAADDLGEEIDWRADGPFDSEEVELGGDGVTRIDLGSVVITPWPGI
                     GLQLQVNEATKQVQAATAVWKNSGLEIVLFAAPASGGLAAELREDAVEEAEQAGGSAT
                     LATGPFGAEVRRVLPQEGPGGEQMFHVSRVWFAEGPRWLLRGTLLGEAAIGEADAPVV
                     APFVELFRNLVVRRGTSPMVPGMLMPMQLPEGPEEG"
     gene            complement(12083..12592)
                     /gene="dut"
                     /locus_tag="PKJ61_01315"
     CDS             complement(12083..12592)
                     /gene="dut"
                     /locus_tag="PKJ61_01315"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016669976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HOC12504.1"
                     /translation="MTSAGTGELRVGLTRLDPDLPLPRYAHPGDAGADLAIAEDCELA
                     PGERRLVRTGIAIAVPEGWAAFVHPRSGLAARSGLTIVNAPGTVDSGYRGEIRVCLLN
                     TDAAAPVRLQRGDLIAQLVFQRVDTAQFELLDELPGSVRGSGGHGSTGGIAEWAVRSD
                     GRTTTEVNK"
     gene            complement(12602..13078)
                     /locus_tag="PKJ61_01320"
     CDS             complement(12602..13078)
                     /locus_tag="PKJ61_01320"
                     /inference="COORDINATES: protein motif:HMM:NF022736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3093 domain-containing protein"
                     /protein_id="HOC12505.1"
                     /translation="MDYSERLRVPPSWWLVGLFFALSFVTAVGFYAGPWVAVVAGAVT
                     AIAVCVALLWFGRLRVAVDADGLHAGDALLEWPYLGAVTALSAAATRERLGAKADPAA
                     WLAVRGYVPGSVEVEIKDPADPHPYWLVSTREPQKLAAALASRASISPDGEASERG"
     gene            13125..13418
                     /locus_tag="PKJ61_01325"
     CDS             13125..13418
                     /locus_tag="PKJ61_01325"
                     /inference="COORDINATES: protein motif:HMM:NF025385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4235 domain-containing protein"
                     /protein_id="HOC12506.1"
                     /translation="MNSSQKMLWNLYAAAVGAATTMVTAKALESAWKLTTGDEPPLPT
                     DPQVPLRRALIWTVASALGVGLAQVVVNRFAANQWVKLTGTPAPSAKRTNVTI"
     gene            complement(13499..13795)
                     /locus_tag="PKJ61_01330"
     CDS             complement(13499..13795)
                     /locus_tag="PKJ61_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007077923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4193 domain-containing protein"
                     /protein_id="HOC12507.1"
                     /translation="MATDYDAPRKNDEENEDSIEELKTRRNDKNSGKVDEDEVEAAET
                     FELPGADLSHEELTVRVLPRQADEFTCASCFLVKHRSQIAEIKGQLIYCVDCAG"
     gene            14021..15037
                     /gene="sepH"
                     /locus_tag="PKJ61_01335"
     CDS             14021..15037
                     /gene="sepH"
                     /locus_tag="PKJ61_01335"
                     /inference="COORDINATES: protein motif:HMM:NF040712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein SepH"
                     /protein_id="HOC12508.1"
                     /translation="MREPPQTTLEIEMDSALTPREIQARIRAGESVEEVARAAGMGLD
                     WIEPFAAPVLAERVFISGQAQSHPVRRGSDTIAHRTLGEVVADRLVSRRVDVDTLTWD
                     AWRLEGRRWVIRVDYDSGKAHREARFIYDAGSRFSVADNDDARWLLGLHSASHGPQPG
                     RRRRDEEAEPTIGLNDDLALVRVVQPAVDEPADPNDTAPLNDLDAFADGDDAFAEGEL
                     TEVDGVYDIVPSSDSGLDLLYDMLSSFDEDSVQIYAGLIRHTDAPEPSPVLDETPVAE
                     PEPAAEVAAAEPAPELTEPHQPALTELEETEPEPQPAPKPKRSKRAQVPSWDQIMFGS
                     PKQR"
     gene            complement(15048..15686)
                     /locus_tag="PKJ61_01340"
     CDS             complement(15048..15686)
                     /locus_tag="PKJ61_01340"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HOC12509.1"
                     /translation="MAELTYFTGPMDCGKSTLALQVAYTESHAGREGSLFTMLDRAGS
                     STITSRIGLSAAAHEVTEDLDFWQHVVGELTAGRRIDFIVCDEAQFFEPAHIDQLARI
                     VDELQIDVFCFGILTDFRTELFPASRRLVELCDRMELLQVRPRCWCGEPATHNARTVN
                     GVMVTEGSQVVVGDTNDAAEVAYQVLCRRHHRRKVTAQVAAVTLSPEPLPFG"
     gene            complement(15734..16843)
                     /locus_tag="PKJ61_01345"
     CDS             complement(15734..16843)
                     /locus_tag="PKJ61_01345"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOC12510.1"
                     /translation="MPPPRLPDYGVSTLADLLPSLGAHLGVPGADDRLGLPPAERYLI
                     ALVDGLGHHQLNEYAEHAPYLSGLSGRVITSGVPSTTATSITSLGTGLVPGQHGIAGY
                     SFWYPAERMVLNTLKWPATLSGLDVQPQLTYFERLASAGVRTATVAPAFFAGSGLTTA
                     AVRGPSFLPVTNERDAARRVELAVQSVGGSGRAVGYFYERQLDHAGHEHGIGSAPWLH
                     ELASADALLAQVRAALPDDVRLVVTGDHGMINVPPASRLFFEDEPDLLAEVTALAGEG
                     RLRQLMTRQPDAVAARWRDRLGTSAWVRTRAEAIEEGWFGAVSPRLADRFGNVLVAMA
                     DDGAVMSRMLPKELTLVGMHASLTPAELDVPLLVD"
     gene            complement(16843..17427)
                     /locus_tag="PKJ61_01350"
     CDS             complement(16843..17427)
                     /locus_tag="PKJ61_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002549009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5998 family protein"
                     /protein_id="HOC12511.1"
                     /translation="MPTRSTIRLPQGLHAEIHACGYFPELVEDAVALALGAEEPTAHL
                     VHHEATFNHDEIQRHLSVLVLTATRLIVAHTDENDNPGEPAQALTTTESVGLRQVNSV
                     ALSQVVAHPERYGTHKSSAVEAWLTISWGTMRRVDLEPAHCADPNCDADHGLTGMIAG
                     DDLTVRISSAGDGNAKLAQLVAFGTALQLATASH"
     gene            17544..18584
                     /locus_tag="PKJ61_01355"
     CDS             17544..18584
                     /locus_tag="PKJ61_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020391020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2183 domain-containing protein"
                     /protein_id="HOC12512.1"
                     /translation="MSTRPFIAARVEEFFDRRVEGLMRALGWRENVICYTGYGSSESI
                     RVLGRVVLVPPWSRTSLGRATEDLMKRRGFRNFISVPCVRPQAMIVVGEHEYPVVADR
                     GGYIDHRVREHGLVGGWQQVGVRSATSPTATADVHVVGDEVTFGIVSDIDDTIISTML
                     PRPMIAAWNSFIRDESARQAVPGMAQLYDRLLAEHPGSPVIYVSTGAWNTHGFLGRFL
                     KRHAFPPGAMILTDWGPTNTGWFRSGMAHKAETLLLLARDFPNISWLLVGDDGQHDPA
                     IYSNFARVAPERVRAIAIRQLTPAEQVLAHGTPSMLPEEELTEPADTPTVAAPDGRGL
                     LAALRGLAEPLA"
     gene            18613..21060
                     /locus_tag="PKJ61_01360"
     CDS             18613..21060
                     /locus_tag="PKJ61_01360"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HOC12513.1"
                     /translation="MVAKPPPAEEELTERIVDVDVTDEMEASFLEYAYSVIYSRALPD
                     ARDGLKPVQRRILYTMDQMRIRPDASHVKCARVVGQVMGQLHPHGDAAIYDALVRLGQ
                     PWSMRLPTVDGHGNFGSLDSGPAAMRYTECRMSPAAAAMTADLDSDTVDFRPNYDGKE
                     TEPVVLPAAFPHLLVNGSTGIAVGMATNIPPHNLVEVVQALKHLMWHPDADVEELMRF
                     VPGPDFPTGGKIIGLDGIRDAYTGGRGVFRVRASARVEQVHARRKGIVVTELPYQIGP
                     ERIIEQVKNLVSSKKLTGISDIKDLTDLTNGTRLVIEVKNGFNPEALLEELYRLTKLE
                     DSFGINAVALVEGQPNTLSLKQMLEVYLDHRLQVVRRRSAFRLGKARDRLHLVEGLLL
                     AIVDIDDVIAIIRSSDDATAARTRLMEAFELSELQANYILEMRLRRLTKFSKLELDSE
                     RDELWLTINELQAILDSEQRLREVAASELDEVARTHGTPRRTVLLASAGTQVTAQSAA
                     NRLEIADDPCWVMLSSAGLLARTDNDEPLPTSGPRANHDVISSAVRTTARGEYCAFTN
                     RGRLIRGQAIDVPAVPVTAAAPNLQGGSLASELLPLDPGETILALGTLGADTFGWALG
                     TQRGVVKRVNPEVLGKDSWEVVRLDDGDEVVGVVELSHDLVELVFITSDAQLLHFPAT
                     SVRPQGRTGGGMAGVKLSRGAKVVFFGVTPTADSSVVTVAGSSAALPGTEAGTVKVTP
                     LAEYPGKGRATGGVRCHRFLKGEDTLLLAWAGPDPVIAAAASGTPIDLPAPDPRRDGS
                     GAPVAQPVAAIGSRYHA"
     gene            21197..21691
                     /locus_tag="PKJ61_01365"
     CDS             21197..21691
                     /locus_tag="PKJ61_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017933777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HOC12514.1"
                     /translation="MDNSFDDLLAANSRYATSAPRGFDGYAHAGVAVVTCMDSRLQPL
                     EMIGLFLGEAKILRTPGGHVTPDALAGCVLGVNLLQVNRILVITHSRCAMASGDDEEM
                     RRRVAAASGADTSGYSFGADPDQFGRLRADVAVLTEHPLIAGHAKVGGFHYDVDSGLL
                     TQVC"
     gene            21815..22024
                     /locus_tag="PKJ61_01370"
     CDS             21815..22024
                     /locus_tag="PKJ61_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC12515.1"
                     /translation="MWWIPSVFAPISLVATWRGAIYSCVGYLVGLLAGELIGEPVYQA
                     QLARPHEQKQDPSYIQNWEPHHPGW"
     gene            complement(22096..24168)
                     /locus_tag="PKJ61_01375"
     CDS             complement(22096..24168)
                     /locus_tag="PKJ61_01375"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002513676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HOC12516.1"
                     /translation="MNTTKPAEGRDYEARHLLVLEGLEAVRKRPAMYIGSTDTRGLMH
                     CLWEIIDNAVDEALAGFGNRIDVQLAADGSILVVDRARGIPVDIEPRTKLTGVEVVFT
                     KLHAGGKFGAGSYNATGGLHGVGASVVNALSSRLDVEVDRNGATYAMSFRRGVVGVFD
                     GDGPNAPFTPGGTLRKVGRAAKGVTGTRVRYWPDRQIFLPNAELSLNQLLSRARQTSF
                     LVPGLELTVTDARSGEAATETFKHDGGISEFCDFLARDAAVTDIVRLQGTDQFTETVP
                     MLDDAGAMTPTDVERTLDVDIALRWGNGYDHTVQSFVNIIATPKGGTHVSGFERGLTR
                     AFAKGFEGTRLLKAGEEVTKDDVLEGMTAVVTVRLAEPQFEGQTKEVLGTPPVTRLVA
                     RIVENELGAFLNSTKAALRTQARAVMEKVVAASRTRVQARAHKEAQRRKNALESSTLP
                     AKLADCRSTDLDRTELFIVEGNSALGTAKLARDSEFQALLPIRGKILNVQKASVGDML
                     KNVECASIIQVVGAGSGRSFDLDAARYSKVIFMADADSDGAHIRTLLATLFFKYMRPL
                     VEAGRVYTAVPPLHRFEMINPRKGQEKYIYTYSEAEYRRKLAELTKKGQPFKEPQRYK
                     GLGEMDADQLADTTMNPRHRTLRRITVDEAGAADAVFEKLMGNDVAPRKEFIIEGAYA
                     LDAERIDA"
     gene            24348..24539
                     /locus_tag="PKJ61_01380"
     CDS             24348..24539
                     /locus_tag="PKJ61_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016667527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC12517.1"
                     /translation="MSATVTEPLTAADRCDRCGAQAYLRVTLNSGGELLFCGHHGRAH
                     SAKLNQVALKIHDETAKLS"
     gene            24610..25128
                     /locus_tag="PKJ61_01385"
     CDS             24610..25128
                     /locus_tag="PKJ61_01385"
                     /inference="COORDINATES: protein motif:HMM:NF001664.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HOC12518.1"
                     /translation="MTIDAVRGDLTREPADVLVNAANASLLGGGGVDGALHAAAGPEL
                     LAACRKVRATSWPDGLPVGEAIATTAGLLPALWVVHTVAPNRHAGQTDEALLASAYRR
                     SLDVACDLGARTVAFPALGAGAYGWDAATAARVAVQAVAAHPRTGLERVRFVLYNEPL
                     HAAFVAELARLA"
     gene            25125..27095
                     /locus_tag="PKJ61_01390"
     CDS             25125..27095
                     /locus_tag="PKJ61_01390"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOC12519.1"
                     /translation="MTAMPYGSWPSPILAADLTRSSVRLSPGRMDAGVRYWTEGHPEQ
                     GGRVGLWRQGPNEPPTELTPEAYVRTGVNEYGGGDWTVQDGVVAYSSWPDGSVQVRTN
                     DGATTELAPGGRFRYAALSLHREFGLLLAVREDHEVAGNEPETTIVALDLAETNPDGG
                     RVLVAGADFYAHPCLDASGRLAWCEWDHPNLPWDSTRIVVAPLDSPADRTQVAGGPGI
                     SALYPAWAPDGALLYLSDASGYWNFERWDGSASRPLFSAPYDFCGPLWVLTPVPYTVI
                     DASRIGCTWLADGVARLGVLAFADPGYAVVPANTVIPAKAGISQRDRWISAEDRPGTL
                     TELGVTAVAADVTGTGVSTLAVLGYADRPAELVELDWITGQTTVVRKASEVDLDQAMI
                     SVAQPFSWESPDGPVHAWYYPPTHADVTAPAGELPPIQVWSHGGPTAFSDPGFALAVQ
                     YWTTRGIGILDVNYSGSTGYGRAYRDRLRGTWGVADVRDCINGAVALAAAGLADGKRL
                     SIRGGSAGGFTTLAALTTSKVFASGISLYGIGDLETLATDTHKFEKHYLDGLVAPYPA
                     GRQTYLDRSPIRHLDQLSCPMLLLQGSEDKVVPPSQAEAMAQAVLAKGLTADLVVFEG
                     EGHGFRRSENIIKVAELALAFLGRVHGFRPAT"
     gene            27255..27905
                     /locus_tag="PKJ61_01395"
     CDS             27255..27905
                     /locus_tag="PKJ61_01395"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HOC12520.1"
                     /translation="MTAVVTHVVWDWNGTLLDDLHCCIDVTNTLLTEYGLPVLDDVAA
                     YQRVFRFPVADYYADLGFDTRPGGNFDTASVRYLELYHAAAAGCELHNGARETLRTLH
                     AAGVRQVVISASEQQNLLKQLSPFELNGWLDGAHGIADIYAASKLSIAQRWLATTGAD
                     PAKVVFVGDSEHDYEIAMALGACCVLFSGGHHSRDHLQTLDAPVVDDLTQVAAHVR"
     gene            28060..28188
                     /locus_tag="PKJ61_01400"
     CDS             28060..28188
                     /locus_tag="PKJ61_01400"
                     /inference="COORDINATES: protein motif:HMM:NF013561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HOC12521.1"
                     /translation="MLNLRVSEDTKAQLEHLAEASGRRQSDLVRDALAEYLERHAS"
     gene            complement(28447..29790)
                     /locus_tag="PKJ61_01405"
     CDS             complement(28447..29790)
                     /locus_tag="PKJ61_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOC12522.1"
                     /translation="MSPRSSAAAVPANEEAATTAPKRRTRKAPAPVTALPNLEPTAVD
                     GPDEDLEPTDDTIDDAEVELDAVTVEDVLAPANIENVKFEREGNDVVLTVGGKKRTLD
                     DVDESAFEPAEAAKTEQELAEDGGFSLSDADDADEPEQQVLVAGATADPVKDYLKQIG
                     KVALLNAEQEVQLAKRIEAGLFAEETLHEGGTGAGPSEDLSWISEDGRRAKNHLLEAN
                     LRLVVSLAKRYTGRGMLFLDLIQEGNLGLIRAVEKFDYTKGYKFSTYATWWIKQAITR
                     AMADQARTIRIPVHMVEVINKLARVQRQMLQDLGREPTPEELAKELDMTPEKVVEVQK
                     YGREPISLHTPLGEDGDSEFGDLIEDSEAVVPAEAVNFTLLQEQLHDVLDTLSEREAG
                     VVSMRFGLTDGQPKTLDEIGKVYGVTRERIRQIESKTMSKLRHPSRSQVLRDYLD"
     gene            29972..32083
                     /locus_tag="PKJ61_01410"
     CDS             29972..32083
                     /locus_tag="PKJ61_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007077945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOC12523.1"
                     /translation="MSQPPLAPRRPSPRRHHGDTFVDHYAWMRDKSDPAFLAYLEAEN
                     AYTEAETAGLADLRQEIFEDISARTKQTDLSVPEFVRHRGLGDFWYYARSTEGHDYPS
                     YHRCPAQGRDSLPHPQAGSPVGEQLLLDAQVLAADSAFFALGTFNVSPDGRLAAYSVD
                     HSGDERYRLVFLDLTTGELLSDELPEVAAGGTWAGNDAFCYLTVDEAWRPEKVWRHRL
                     GVTGPDELLFTESDERFWLGVDSSRDDRWLLVASASKTTTEAWLLDLVDPDAELRCVR
                     PRVEGLEYDVEVASDRLFIVHNQDAPDFALAQAPLTAATGADWTPIWAGEPGVRLLGV
                     TAYDRVLVASVRRDGLQLVTLRYRDANGDVGPETAIAFDEPLYQLDADDGDEADTDRI
                     RLHYQSMVTPDQVIEYRLTDGARKVLRQRPVLDHPRFGAYDPSAYVQRREWATAPDGT
                     RVPISLVHRADTPLDGAAGCLLYGYGAYEISIPPTFSIARLSLLDRGYVYAIAHVRGG
                     GELGRAWYDGGRLAAKTNTFTDFIACARHLVAAGYTSADRLAAEGGSAGGLLIGAAIN
                     LAPDTFAAVHAAVPFVDALTTILDPELPLTVMEWEEWGDPLHDPDVYRRMRGYSPYEN
                     IRTGEYPAILVTASLNDTRVEITEPAKWVAELRHTSGSTRPIHLRTELTAGHGGNSGR
                     YQGWRDAAFELAWLMAHTSQG"
     gene            32214..33161
                     /locus_tag="PKJ61_01415"
     CDS             32214..33161
                     /locus_tag="PKJ61_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013161174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOC12524.1"
                     /translation="MATAHRIRSTDGIDGKDAVGLYLDGIAKTPLLTAEEEVQLAKLI
                     EAGRYAHAILDGTLDDAESALCRVRATEDELTEIARQGDAAMQRFITANLRLVVSVAR
                     KYGRAQMPLLDLVQEGNTGLIRAVEKFDYTKGFKFSTYATWWVRQSISRGIAQQGRIV
                     RLPVHVAEQVNQVSAVRRTLERRWGREPELVEIADELGLTEEQVIDLLRLSRDHVSLD
                     APVEEDGDTALGDLLAREMSPGPDEVVLDAEDRARLEAMLATLDDRSADVVRRRYGLL
                     DGRQAKLADIAAVWGITAERVRQIERHAISKLRGDAEAA"
     gene            complement(33249..34124)
                     /locus_tag="PKJ61_01420"
     CDS             complement(33249..34124)
                     /locus_tag="PKJ61_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015071691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HOC12525.1"
                     /translation="MPESVDELIALLDLEQTGEATFLGQDDDQEGRMFQRVFGGQVLA
                     QALTAGYRTVPAGRVAHSLGAYFLRPGAPGVPITYSVEKTRDGGSFSARRVVAAQDGR
                     VIFSMVCSYHELEAGLDHADQAPLGVAPPEDCPPLSEVLGARSRRAVAAWRQEWGVLE
                     TRFASDTSVATAAAHADADMKVWVRAESALPDDPQIHQAVLAYASDLTLLAVSTVPHP
                     VEFGGPNMQVASINHSMWFHRPARADQWLLYDQVSPSASAGLGFSFGRLFADGRLAAS
                     CAQEGLIRIVDPSSS"
     gene            complement(34127..35020)
                     /locus_tag="PKJ61_01425"
     CDS             complement(34127..35020)
                     /locus_tag="PKJ61_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HOC12526.1"
                     /translation="MLDPRLLYSLDPELWDSVRGAEPALIHLLDGYIDAGSITHGTGR
                     YLLEVCEHEVLVEFDHDQLHDYRSRRPQMVFDTDHWVGMTDYALVIHKLKDAVGKPFL
                     MLSGPEPDTQWNRTASAVLGIAEQLGVSRMLTVSGVPMAVPHTRPTLVTAHATQPETV
                     SGNPMWIDRVQIPGSFSHMLEFKAGEAGILAGGFVAHVPHYLAQTSFPQATEAVVQRL
                     ADAAGLEIPMLALAERAAANLASIASETAEDAEFPALVATLEEQYDRLSSSGAASMPS
                     ADEIGAAVEQFLAEQDPPNGR"
     gene            complement(35081..36055)
                     /locus_tag="PKJ61_01430"
     CDS             complement(35081..36055)
                     /locus_tag="PKJ61_01430"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOC12527.1"
                     /translation="MPYDSAVLDLNRLRILRSVVASGSVNDTARRLGLTPSTVSQHIH
                     TLEREVGFTLVERVGRGIQPTPAAIELALTSQDALAAMTKVDAKARELRQGATAKLTM
                     RTFASAAYTWMPTVARTLRQEFPGLTLELSINETDSPELAGQADVEVHTELPYEEPRV
                     PPGYRRIELGTDDLLVAMPPDHPLAEAGTMDLAEFSTDDWVHYDFRDDIATSLASHAC
                     AGAGFTPRYVVRAQDHVTGLAFVAAGVGIALVPQLAVGWSGFEIAYVRPTNPTPYRRI
                     VALLRNGAASFPAAARTIELLTDLGGRIRELQPIVAPTSAAEPDTHDS"
     gene            36134..36292
                     /locus_tag="PKJ61_01435"
     CDS             36134..36292
                     /locus_tag="PKJ61_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC12528.1"
                     /translation="MTDRDRERLIEDLELARQIEPTPMVRRKVDKRHWFRIAMTEGET
                     IRTHFASV"
     gene            complement(36296..>39439)
                     /gene="hrpA"
                     /locus_tag="PKJ61_01440"
     CDS             complement(36296..>39439)
                     /gene="hrpA"
                     /locus_tag="PKJ61_01440"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HOC12529.1"
                     /translation="QLDAICDAVTELSTQLDGDILVFLSGEREIRDAADAINGLNLRF
                     TEVVPLYARLSVAEQQRVFAPHTGRRIVLATNVAETSLTVPGIRFVVDPGFARISRYS
                     ARTKVQRLPIEPISQASANQRAGRCGRLGPGVCIRLYSEEDYAARPEFTEPEILRTNL
                     ASVILQMAEAGLGEITNFPFVEPPDATQVRDGLRLLTELGAISGMGKGTVNLTPTGRQ
                     LARLPVDPRLGRMLVEASRLGCLREVLPIVAGIAIPDVRERPSEETAAADLAHRRFWV
                     PMDGGGAAEQAQEPTPDGSDIAALLRLWQYLRQQRRELSGNAFRRMCRSEYLNFLRVR
                     EWQDLHTQLREACRELGLSRNDATADLASVHTAVLSGLLSHVGLIDAASTAKPERGRR
                     RGPAEYLGARGARFAINPGSSAARTNPPLVMAVELVETSRLWARTVAPIDADWVEAVG
                     GHLLKRNYSEPHWSAKGGQCVAYERVTLLGVPIIAQRLVSYARIDPVVAREVFIQSAL
                     VEGQWRTRHHFWARNQAVRAEAEDLADRNRRRDLLVDDASIAAFYAARIPAEIVTVAH
                     FDRWWRDARRKDEHLLDLTVADLLAPDAELDTTSFPDHWPVAAEELAVDYAFDPGSGH
                     DGVTVTIPLAVLPKLNPAAFTWQVPGLRTQVAAELVRGLPKALRTQLVPAPDTAARAL
                     DWLADHPGGADEPIWIALARGILALTGVQVPPDAWRPEAVPEHLLVRFRITGAGREPE
                     IGRDLAALAERLAPKVSRKLNASAAHLTHPGATSWQFGSIPQRLGDELTGYPALVDRV
                     QQVGVQVFADEADARRAHRAGLRRLISLATVDPTRWVVSRMSNPTKLALATSPYPDVP
                     ALLADARLRAIGDLMDAVGGLDQVRDEAAFNRLADAVRAQAAATMQQVVQATGEILVA
                     AGEIRQQLPTATDRTRQDVAEQLNGLVFAGFVAAIPPKWWHRLPTYLAAVGRRLVAAA
                     SNPRHEAEGLATITELEEEYALLCAGFPSGPLPEPVAEVGWLLEELRVSLFAQALRTS
                     VPVSPKRLRTAMAAAAR"
     assembly_gap    39440..39539
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<39540..39892)
                     /locus_tag="PKJ61_01445"
     CDS             complement(<39540..39892)
                     /locus_tag="PKJ61_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC12530.1"
                     /translation="MTADPVLTFDEALPISAHVEEIRDALRNHQVIVVAGETGSGKTT
                     QLPKICLLAGRRKIGHTQPRRIAARSLATRIAQEMGEAVGQTVGFQVRFSSQRSRQTR
                     VKVMTDGILLAEISH"
     gene            complement(39923..40822)
                     /locus_tag="PKJ61_01450"
     CDS             complement(39923..40822)
                     /locus_tag="PKJ61_01450"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOC12531.1"
                     /translation="MLKPYEGPARVVVGFDGSEDAARALEYGIVEARHREAELVVVHA
                     VDDTVLNSAWGVVFDPEEIKLGAAEMLASTIDVAVAHGLERGRVRTQVVLGNTAAALT
                     RMSSDASLVVVGRRSTSTGEKAFVGSTAVGVVGAAHCPVVVVSSTDVVRETPAGLIGV
                     AVDTSARGAVALEWALRECQSRGGKVSVISVCRAPQGRWFSGGHPTAEQQAAAVKVTR
                     QRMTEMIQPLAEAFPDVAVELEVEYGVPLDILAARTAELDLLALEVHAAFPTYSVGGL
                     IRGLMSHGSCPIAVIRPKDSHGS"
     gene            complement(40824..41228)
                     /locus_tag="PKJ61_01455"
     CDS             complement(40824..41228)
                     /locus_tag="PKJ61_01455"
                     /inference="COORDINATES: protein motif:HMM:NF024304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOC12532.1"
                     /translation="MDTAGHFTRPSGEECRQLVRSHCVGRVAWLAASGLQVLPVTYGC
                     HDDRIFFRVNPASVMGELADDVEVAFEVDDIDVDTATGWSVLVHGVAADHDGSAQPVL
                     PAPWAPDHSGLTIVITPSTYSGRAVSAAVERN"
     gene            complement(41317..42252)
                     /locus_tag="PKJ61_01460"
     CDS             complement(41317..42252)
                     /locus_tag="PKJ61_01460"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HOC12533.1"
                     /translation="MQAALSGYLTIWSVIGVGWVVAHFRVLTEDHRRMLSRLAFTVAS
                     PALLFSLVSRADLSHLFSRTLLVSALAIALAAGAYLIAARVWFGGGDLPGRVVGTLCA
                     AYTNAGNLGLPIAAYVLGDMTWMAPIILIQVGVLQPTALALLDLGAARKDGVRLTWWR
                     YASLPVRNPITLGTLAGLAVNLLGIEVPTLLWEPIEMIGQVAVPAMLLAFGISLRLDP
                     LPGRGRHARELALAAPIKVLLHPAIAFALGAWVFGLSTADLLAVTVIAALPTAQNIYL
                     IAGRYHVRTLLARDAIFISTIASIPVIVAASTWLR"
     gene            42321..43580
                     /locus_tag="PKJ61_01465"
     CDS             42321..43580
                     /locus_tag="PKJ61_01465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC12534.1"
                     /translation="MAERNHLIDLARITSVLVVVVFHTLLWVVAVPDGQLLIYPWAPG
                     PGWWAASWIFNIVPVFFVAAGYANAVVVDKWRTSGAPYSAFLVLRGGRLLGPMTLFVA
                     VFTVVGTVAAWAGWPMESATLSRQFAQLLWFAVVYQLLLAASPLAVWLHDRFGGWVML
                     PLLLGVIAVDAAVRASGDLGLQWLNLAFVWPLAHQWGIAYHRGWFRGWRTRTLLALLA
                     GCAAVIAALVFALDYPPASVAWADVLVANLLPPTVAILVLGLAQTVVLALLEQAGVGV
                     CLRAGTQQLVRVANALLLTVYLWQIPAILVGAGALAGLSLLWPAAAPVLMNRVLLVVL
                     VLGIVVTAVPWLARVELRLIPASGNGIAKPGHAEIGYALSCTGTFAVWQAGAVLHPSA
                     PWAAAAAGLFAAGVMALRRVSASTVVG"
     gene            43607..44122
                     /locus_tag="PKJ61_01470"
     CDS             43607..44122
                     /locus_tag="PKJ61_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HOC12535.1"
                     /translation="MDPTQWHVPYPLVVAALFVIVMLRANGTYWLGRAASAGARHTRL
                     AKLMDSPGYLRATQRIDAYGPPVISLSFLTIGFQTLANLAAGATRMSLRHYLPAVVIG
                     SVMWAFLYATLGTVGVDLLGDLYRQSPALAIGLAVLVMALIAAYVIWQVRRRTLAGRV
                     PLAEDTALAED"
     gene            44161..44370
                     /locus_tag="PKJ61_01475"
     CDS             44161..44370
                     /locus_tag="PKJ61_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002549702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC12536.1"
                     /translation="MAEIRKYDPAKGNAIVEALRSAMGDPIRPVTPGEPAPTRKPSVA
                     PATTTSWVTFNVAQASASDEAADQD"
     gene            complement(44459..>46447)
                     /locus_tag="PKJ61_01480"
     CDS             complement(44459..>46447)
                     /locus_tag="PKJ61_01480"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002527352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HOC12537.1"
                     /translation="NNSVRVSDEFMQAVEDGTDFGLRARMTGEVIEKVDARELFGKMA
                     KAAWECADPGIQYDDTINAWHTNPESGRINASNPCSEYMSLDNSSCNLASLNLMKFLR
                     DDDTFDVESFVKAIELIITAMDISICFADFPTEAIGETTRSFRQLGIGYANLGALLMA
                     IGRGYDSDAGRALAASITSLMTGVSYRRSAELAAVVGPYNGYARNAAAHQRVMRKHQA
                     ANDDVRIWDELDKPLHMAASEEWAQVVALGAKNGYRNAQASVLAPTGTIGFMMDCDTT
                     GIEPDFSLVKFKKLVGGGSMQIVNQTIPRALQRLGYAGETVEAIVDYIANHGHVVNAP
                     GLKPEHYEIFDTAMGQRAIKPMGHVRMMAAVQPFLSGAISKTVNLPETATVEEIADVY
                     AQGWKLGLKALAVYRDNCKVGQPLSDGKKTDEPAAKAEPEVRVEYRPRRNRLPKSRVS
                     RTTSFSVAGAEGYMTSGQYNDGKLGEVFLKLGKQGSTLAGVMDAFSIAVSIGLQYGVP
                     LEQFAQKFTNLKFEPAGMTDDPDVRMAQSIMDYIFRRLALDYLSFDERAELGIYTAAE
                     RARYVETGRYLSEEDEAELIESESLKNDAADDVRVHEDGPNQPALVEFKAHSTAEVLE
                     QLSGMSVDAPLCLTCGTKMRRSGSCYICEGCGSTSGCS"
BASE COUNT         7446 a        16031 c        15462 g         7408 t
ORIGIN      
        1 cgcagtctgg tctcgatgcc gagagcacgc gcctcctggc ccagccgggc acccatgccg
       61 ccgaccacgc cgttgtcctc gatggtgacg accagctcgt gcgcggccat cagggcgagc
      121 aggtcgggtg aaaccgggag tgcccagacc gggtctacca cggtcgcgcc gatgccctgc
      181 tgggcgagcc ggcggcccac gtccactgcg gtgtcacaga gctgtccgta accgaccacc
      241 agcacgcgcg gacgctggct ggcgaacagc acgtccacat cgccccgctg ctgcacggcc
      301 gggatcggtt cgggcagacg ctccttcgag tatcgcaaca cggtcggcgc gtccgagacg
      361 gtgacggcca gccgcagcgc ctccccgaga cgttgctcgt cccgcgggga ggtgagccga
      421 agccctggca ccaggccgac cagggacgcg tcccacatgc cgttgtggct gggtccgtcc
      481 ggaccggtca cacccgcgcg gtcgagcacg aaggtgaccc cctggcgatg cagggcgacg
      541 tccatcaaca cctggtcgaa ggcacggttg aggaacgttg cgtacacagc gaagaccggg
      601 tgcatcccgc cggccgccag gccagcggca gccgtgaccg catgctgctc ggcgatgccg
      661 acatcgaagg tgcgctccgg gaaatgccgg gcgaacttgt ccagtccggt cgggtgcagc
      721 attgcagcag tgatcgcaac cacggactcg tcgatggctc cgatgcgggc caactcgtcg
      781 ccgaaggcgt ccgtccaggt gcgcgccggg gtgtcggtga gcggttcgcc ggtgacctcg
      841 tcgatctgcc cgaccgcgtg gaaacggtcc tgctcgtgct cctccgcagc cttgaagccg
      901 cggcccttct gggtgatgca gtgcaccagc acaggcccgc cgaagccctt cgcctgcgcc
      961 agcgccagtt ccaccgcctc gatgtcgtgg ccgttgatcg ggccgaggta cttgatgccc
     1021 agatcggaga acatgccttg tggcgcgagc acgtccttga cgcccatctt gaacccgtgc
     1081 agcagcccgt agaccatctc gcccaccagc ggagtcttgc gggccagttg cttgattcgt
     1141 ttgagcgtcc gctcgtagcg gcggtcggta cgaatcgcgg ccagctgctt gccgagcgag
     1201 gagagctggg tggcgatccc gccgacggtg ggcgtgtagg agcggccgtt gtcgttgacg
     1261 acgatcacca gccgctcgtc gccggagact gcgatgttgt tcagcgcctc ccagaccatg
     1321 ccgccggtga gtgcgccgtc gcccacgacg gccaccacga agcgatcgtc accacgcaga
     1381 cggaaagcct tggccagtcc gtcggcccag gacagcgcag tggacgcgtg cgagttctcc
     1441 acccagtcgt gcggagactc ggcgcggctc ggatagccgg agagtccacc gggctggcgc
     1501 aggctcccga actgatcctt gcgaccggtg agcaccttat gcacgtagct ctggtggccg
     1561 gtgtcgaaga cgatcgcgtc cttcggcgag tcgaagatgc ggtgcagcgc gatggtcagt
     1621 tccaccacgc cgaggttggg gccgaggtgt ccgccggtgc ggctgacgtt ggtgatcagg
     1681 aacgcgcgaa tctcttcggc cagttgctgg agttgctcag gggtcagatc gctgagggcc
     1741 tcagggccgt tgatctcgtc gagcaggctc accggacgag tctatgtcag agcttgggcg
     1801 tgaacactct gccacgcaaa gcctcgacaa ccatgcccac ctcccggcgc agccggacca
     1861 gacgcgctcc ctcggtcgtc cacgcttcca cggcttcctg ggcaacttcc ggacggacgt
     1921 agtcggccag ccccgtccgc acccgggcca ggccctcgac gctcgcgcga gcctgcgagt
     1981 cgacgaactc gacggcgaac attccgagca ccaccggatg ccgccgcaac accggatgag
     2041 cacggtaatc ggccggacag cagtcgaaaa ggaacgccgc agcggattct tcccactccg
     2101 cactgcccgg tggccggacg cggtcgggcc accccggcgg cgcaaacccc ggacgcggat
     2161 tcggccgcgt cggttcggga gccttccgtc tgatccagag ccgttcggga tacatgtgtt
     2221 cgattatagt cgagacgagc gttcggtgct cggatctcta gcccaggtac caccagatca
     2281 ccgcggccag ggacagcccg gccagcacca gcagtgcgac ctgctgcgtc cgccggaatc
     2341 gcggcccgcg atcccaatcg gcgctcgcca gcgcggccag agcgggcagc aacgcggcca
     2401 ggacccacca ccaccaggcg gtgatcgcgt ccactgcgac cagcagaccc gccccggcga
     2461 gcagggtcag agccagccac cagtccagtt tcagcgcacg aaccgctgcg accagcgcga
     2521 cgaggaagcc gccgatcgcc agccagatcc cccaccagaa cagacaactg atcgcaaaat
     2581 cgtccgggct gtcgtcgacg accaccttgc agaccgtgcc gctgaccacc tggccgccga
     2641 ccaccaccag cagtgcagca cccaccacag cgatcagcgc ggccacgtac gtccacgcca
     2701 cccgggcgga gaccggcatg aagtccggct ccaccgcggc tggctggacg cgcgcccgcc
     2761 gccggcccac tacttgttca gcagcgaccg cagcacgtat tgcatgatgc cgccgtggcg
     2821 gtagtagtcg gcctcacctg gggtgtcgat ccgcaccacc gcgtcgaaga cgatcggctc
     2881 accgtcaccg acggcctcca cccgaacggt ggccggcgtc gtgccgtcgt tgagttcggt
     2941 caccccggtc accgagaagg tctccgtacc ggtcagcccc agcgaatcgg cattctctcc
     3001 ctcggggaac tgcagcggaa gcacgcccat gccgatcagg ttcgaccggt ggatgcgctc
     3061 gtagctctcc gcgatcacga ccttcacgcc cagcagagcg gtgcccttgg ccgcccagtc
     3121 acgggacgag ccggagccgt attccttgcc cgccagcacc accagcggca ccccggcgga
     3181 agcgtaattc tgcgccgcat cgaacacggt ggccaccggc gcgtccgcca ccgtgaagtc
     3241 ccgggtgaag ccaccttcgg tgcccggtgc gatctgattg cgcagccgga tgttggcgaa
     3301 cgtgccccgg atcatcacct cgtggttgcc gcgccgcgag ccgtaggagt tgaagtcctt
     3361 gcgctccacg ccgtgctcgc tcaggtagcg tccggccggg ctgtcggcct tgatcgagcc
     3421 ggccggcgag atgtggtcgg tggtcaccga atccccgagc ttgagcagca cccgcgcccc
     3481 agcgacatcg gtcaccgcca cgggctcggc cggcatgttg tcgaagtacg gtggcttgcg
     3541 cacgtacgtg gagtcggcgt cccagctgaa cacgtcgccg gtcggcgtcg gcagcgaacg
     3601 ccaggtctcg tcgccggcga acacgtccgc gtagcgcgaa gtgaacatct cggcggtgat
     3661 ggacgagccg acgacctcgt cgacctcagc ctgcgacggc cagatgtcag cgaggtagac
     3721 cgggttgccg tccgagccag tgccgatggg ctcggtggtc aggtcgatgt ccatccgtcc
     3781 ggcgagcgcg taggccacca ccagcggcgg gctggccagg tagttcatct tcacgtccgg
     3841 gttgatccgc ccctcgaagt tgcggttgcc gctcagcacg gacgtgaccg ccagatcggc
     3901 gtcgttcacg gcctgcgaga cctcggggat cagcgggccg gagttgccga tgcaggtggt
     3961 gcagccgtag ccgaccaggt cgaaatcgag ggcgttcagg tacttcgtca gcccggactt
     4021 ctcgtagtag tcggtgacta ccttcgagcc cggcgccagc gacgtcttca cccacggctt
     4081 gcgctccagc ccggcctcga cggccttctt cgccaccagc gccgcaccga tcatcacgct
     4141 cgggttggac gtgttggtgc aggacgtgat cgctgcgatc gtgaccgcac cgtggtcgag
     4201 ggtgaagctg gtgccgtccg ccagcgtgac cggcgtcggc ttgctgggcc ggcccagtcc
     4261 cgccggagtg tgctcgccgt actcgtgcgg agccggcgcg ccgttcccac cgtcgtgcgc
     4321 aggcgcgtcc gatgccggga agctctcggc cacggcttcg tcgtacccgg agatgtcgtc
     4381 acccacgtag tcgcgaaggg cctcgttgaa gccctccttg gcgtgcgcga gcaccacgcg
     4441 gtcctgcgga cgcttcgggc cggcgatgct cggcaccacg gtcgccaggt cgagctcgag
     4501 gtactcggag aaccgcggct cgctggccgg gtcgtgccac aggccctggg ccttggcgta
     4561 ggcctcgacc aattccagct gctcggccga gcgtccggtg agccgcaggt agtcgacggt
     4621 cttgtcgtcg atcgggaaga tcgcgatggt cgagccgtat tcggggctca tgttgccgat
     4681 ggtggcgcgg ttggccagcg gcacttcggc cacgccgtcg ccgtaaaact cgacgaactt
     4741 gccgaccacc ttgtgctgcc gcagcatctc ggtgatggtc agcaccaggt cagtggcagt
     4801 ggtgccctcg ggcagcttgc cactgagctt gaagccgacc acgcgcggga tcagcatgct
     4861 gaccggttgg ccgagcatgg ccgcctcggc ctcgattccg cccacacccc agccgacgac
     4921 gccgaggccg ttgaccatgg tggtgtgcga gtcggtgccg acgcaggtgt ccgggtaggc
     4981 ctgcagcaca ccgtccacca cgcgcgggaa gaccacgcgg gcgaggtgct cgatgttgac
     5041 ctggtggacg atgccggtgc ccggcggcac cacgacgaag tcgtcgaagg cggtctggcc
     5101 ccagcgcagg aactggtacc gctcgcggtt gcgctggtat tcgagctcaa cgttgcgggt
     5161 gaacgcgtcc ggggtgccga aaaggtcggc gacgaccgag tggtcgatca ccatctccgc
     5221 cggggacagc gggttcacct tggccgggtc accaccgagt tcggcgatcg cctcgcgcat
     5281 cgtggccagg tcgaccacac aggggacgcc ggtgaagtcc tgcatgatca cccgggcggg
     5341 cgtgaactgg atctccttgt tcggctcggc ggacgggtcc cagccggcca gggccgcgat
     5401 gtccgtggcg gtgatgttgg cgccgtcctc ggtacgcaac aggttctcca gcaacacctt
     5461 gaggctgaac ggcaacgtcg ccgagccctc aaccgcgtcg acgcggaaga tctgatacgt
     5521 cttgccggca acggttaact ggtctgctgc ggagtagctg tcgatgctca tcggtcctcc
     5581 tgaccgccga agatatcttg atgtcgagat attatcccac ccgacgcgat gaggctagct
     5641 gagcgcgccg ttctcagtgc ggcttccgcc gtcgttcgcc ccactccaac cgctgatccg
     5701 ggtcggacat caccaggtag accaggccac agtcctgcgg accggtgacg ttcagctggc
     5761 agaggttgca gatctcttct ggacggagcg ggcacttggc ttcgggacga gtgcgcttgg
     5821 ggtgggtgcc tggggtgttc ataccaactc caggataatc ctcgcacccg tagtctcgat
     5881 cctgcctttc gaacgcatcg cccggccgtg aacgctgtgc agcccgggcg aagccggatt
     5941 tctggagctg atggcgcact ccggattggc cgattggccg ccaaccggaa ttctggagcc
     6001 taagccgcgc tttccggcct gcataagctc cacaattcgg gttgggctgg agtcgggggc
     6061 aagccaccta ctcataagct ccagttctcg ggttgcgctg aggatggact gtccaggcga
     6121 aggtgggcct gcccgaccaa gcacgtcccc gcagatcaac tcaagttgac gccggtacca
     6181 ggatggcaac gcactccacg tgatgggtca tcgggaagag gtcgtaggcg tcaactgaca
     6241 tggtgcgata gcccaggtcg gcggcgatct ggaggtcgcg gccgagggcg gccggatcgc
     6301 acgccacata ggcgatccgc cgcggccggc gggcgaccac cgaagccagg acggcacgtc
     6361 ccgcacccgc ccggggcggg tccagcacca cgatgtcggc acgatttggc agccgctcca
     6421 ggcgacgtgc gacatcaccg accaggaacc gggcggacgg cacgttcgtc cgggccagtt
     6481 cgatcgcccg ccggtcgcct tcgatccccc agacctgcgc ccccgcgtcc gccagggctc
     6541 tcgcgaacag cccgaccccg caatagagat ccagcgcggt ctcccccgct cgcggctgca
     6601 gcccgtccag cacgcgttcg gtcagcactg cgggcgcggc ggcgtgcgcc tgccagaagc
     6661 cggtgagcgc gacctcgtag gagcgcccgt cgacctgctc ggtgaccgtt tccgcggagc
     6721 gctcaacccc gcagaaccgg acgccggagg ctgccgccac cgcgagcagt tccgtccccg
     6781 gtcggcccgg atcggccggc gggtgggcaa tcggcggagc ggcgatccgg cagccggctt
     6841 ccgggagcgg cacgaccgcg tccgagcgat gcgcgagcag cccgggccgg ccaacggcgt
     6901 ccaggtggta gcgcatccgc cgacgccagc cgaacggagc cggtggcacc tcgacgacat
     6961 caccgatgaa ctggtagccg gccaggtgtc cgagcagttc ggccaccacc tgccgcttca
     7021 gttcccgggt gtgctcggga cggacgtgct ggaagtcaca gccgccgcaa cgcccggcga
     7081 tcgcacaggg cggactgacc cgctccgggc tggcgcggtg caccgcgacc acctcaccgc
     7141 gagagaaacg cttgcccgcc tcggtgatcc gtacgtccac cacctcgccg ctcagggcat
     7201 gccggacgaa gatcacccgc ccgtccacac gggccacgaa gtgtccgccg tgggcgaccg
     7261 gaccgatctc cacctcggcg agcacatcgt ccaccgcgaa cggctcggtc actcgggcag
     7321 ttccggtcca cgcgagtcgc cgacgcgcac ctgtccgcta cctggacggc gccaggccgc
     7381 agccgtattg cgcttcgcgc ggtcgatggc gtacttcgag gacgccagct gatagggcac
     7441 cgacgtgacc atcacgccct gcatgaagtt gagccgggtc ttcaggatca gcccggtctg
     7501 gttgtgcagg aattgctccc accagtggcc gacgatgtac tccggaatgt agacgcagac
     7561 cacgtcgcgc ggattgtccg aacgcacgcc cttcacgtag ttcaggaacg gtccggtcac
     7621 ctcgcggtac ggcgaggcga tcaccttcag cggcacttcc agctcctgcg cctgccattg
     7681 ggagacgatc gagcgcgtct ccgcgtcgtc gatcgagacc gtcaccgcct cgatgctgct
     7741 ggctcgcgtc gccctggcga agttgaccgc acgcatcgtg ggcttgttga cctcctgcac
     7801 catgatcacc gcccggacgc ggctgggcag cagccgggcg tccatcgggc tgaccgcggt
     7861 ctcccgtgcc acccggtcgt agtggttccg gatggcccgc atcaccacga agacgagcgc
     7921 catcgcgagc accgcaaggt aggcgccgta gatgaacttt gagagcagca cgatcaccag
     7981 cacgatgccg gtgccggtga gaccgaccgc gttgatcgcc cgagagcgct gcatggtcgc
     8041 gcgcaccttg ggatccttct cggtcttcag atggcgggtc cagtgccgca tcatgccgaa
     8101 ctggctcaca gtaaacgaga cgaagacccc gaccacgtac aactggatca gcgcggtcac
     8161 gctggcgttg tagatcagca ccagcgcgat cgcggcggtc gcgagggcga ggatgccgtt
     8221 ggagtaggcc agccggtcgc cgcgggtgtg cagggcgcgc ggcaggaagc cgtccctggc
     8281 gaggatcgag ccgagcacgg ggaaaccgtt gaacgcggtg ttggccgcaa ggaacaggat
     8341 gatcatcgtc atcgtgatca cgaagtagaa gccggcgggg aaggtgtcga agatggtccg
     8401 cgccagctgg cccatggccg tctcgtgggc cacctccaaa ggcaccccgt tctgggcgaa
     8461 gtgcgacgcc ccgtccgggt cgatcagctt cagcccggtc aggttcgaca gcgcgatgat
     8521 gccggccagc atcgtgatcg cgatcccgcc gagcagcgcg agtgtgctgg cggcgttgcg
     8581 gctcttcggc tcccggaacg cggggacccc gttcgagatg gcctccacgc cggtcagcgc
     8641 cgcacaaccg gacgagaacg cgcgcgccag cagcaccacc atcgccagtc cggcgaacgg
     8701 ctcgaaggcc ggcgcaccga tcacctcgta gttggcggtc ggagcctgca ggtgctcgcc
     8761 catgccgaag atgcggaaca gcccccagat cgtcatgccg agcacgccca ccatgaaccc
     8821 gtaggtcggg atcgcgaaga acgaccccga ctcgcgcacg ccgcgcaggt tcatgctcat
     8881 cagtaggacg ataagtccgg ccgcccacag cccctcgtgc ccttccagga aggggaacat
     8941 cgccttggcg ttcatcacgc ccgacgacac cgagaccgcg acggtgagca cgtagtccac
     9001 gagcagcgcg ctggccacga ccaggccggc gttcgggccc atgttgacgg tcgccacctc
     9061 gtagtcgccg ccgccggacg ggtaggcgtg cacggtctgc cgataggaca gcaccacgat
     9121 cagcatcacc aggccgaccg caagggcgat ccatggggag aaggcgaaac cggccatgcc
     9181 ggccagcgag agggtgagca ggatctcgtc aggggcgtag gcgaccgagc tcagcgcgtc
     9241 cgaggcgaag actgggagcg cgatgcgctt cggtagcagg gtttcgccca actgggcgtt
     9301 ggccagtttg cgcccaacca gcacgcgctt gaccgagtcg gcggcattca cgggatcaga
     9361 ccctatgccc aaacggtgaa gtttcaccca cctgaccggt gtagcgtcgt ttcaggactt
     9421 ccgcaagcgg tagggtcggc ggcgtcaacg acgcaggagt ggaggcacgc ggcgtgcaca
     9481 tcgtcatcat gggttgtggc cgggtcggcg cttcgctggc gcgttcactg gaacgccgcg
     9541 ggcactcggt cgcggtgatc gacatcgtcc aggacgcctt ccgccgcctc ggccccgagt
     9601 tccagggcaa gaccgtcaag ggggttgggt tcgaccggca ggtgctggtt cgcgccggca
     9661 tcgaacgggc ggacgggttc gcggccgtct ccagcggcga caactccaac atcctcgccg
     9721 cccgcgtggt gcgggagacc ttcgaagtgc acaacgtggt ggcccgtatc tatgaccagg
     9781 gccgcgccga ggtgtacgag cggctgggca tccccagcgt cgccaccgtc cgctgggcgg
     9841 ccgaccaggt gctgcgcaag ctgctgccgt ccggttcgga gccacagtgg cgcgacccgt
     9901 ccggcagcgt ccggttgatc gaggtgcacg cgaactccga ctgggtgggc accaagatca
     9961 gcgcgatcga agccgccact cgcgccaaga tcccgttcct gatgcgcttc ggcaccggca
    10021 tcgtgcccaa ctcggcgacc gtgttccagg acggcgacct gatctacgcc gccgtgacca
    10081 acgagcaggt gcccgacgtc gaggacgtcc tggccgaccc gccggtccag cactgaaaga
    10141 ggaagcgacc atgcgcgtag ccattgccgg agccggcaac gtgggccgtt cgatcgcccg
    10201 cgagctgatc gccaacgggc accagatcct gctcatcgac cgcgatccca acgccatcaa
    10261 acccgaaagc gtcccggagg ccgagtggtt gctggcggac gcctgtgagc tcaccagcct
    10321 cgaagaggcc agcctcgact cgtgcgacgt gtcgatcgcc gcgaccggtg acgacaaggc
    10381 caacctggtc cactccctgc tggcgaagac cgaattcggc gtgccgcgca cggtcggccg
    10441 ggtgaaccac cccagcaacg aatggatgtt cgatgacctg tggggtgtgg acgttgccgt
    10501 ctccaccccc cggttgatgt gcgccctggt cgaggaagcg gtgactgtcg gcgacctcgt
    10561 ccggctgttc agcttccgcg gcgggcacgc gaacctggtc gaaatgaccc tgccagacgg
    10621 ttcgccgcgg gtcggcgtcc gggttgccga cctcaatctg cccggcgacg cggtgctggt
    10681 cacggtgatc cgggacggtt ccgcccgcgc ccccgaacgc gacgccgcgc tggaggccaa
    10741 cgacgaactg ctcttcgtct gcgacccggc caatgaggcc gaactggctc aatacctggt
    10801 gcctcgcgcc cgagtgacgg aagcgggcta gggctaggtg gccggacaac ccgcccaatt
    10861 cgggttttcc tgcgacagaa gcgccaggat cagcagtccc ctggaacggg cttcgccagc
    10921 ctcagctgtc gttcggacgg tagctgagcg cgcagctcaa ttcgcgatca gctcgtagta
    10981 ggggttgtac agccgccgct cgccgtccac gtaggacacc cggccgctca cggcgagttg
    11041 cgcgccggcg acgatcccgg ggatttcccg tcgccccatc cagatcaggg tgagtgtgcc
    11101 ggagccgtcg ctgaactccg cctcaagcca gggggtgccg ttgcgcggtt tcatcgcgag
    11161 caccgtgatc gtgccgcgca accgggccag ctgacgatcc tgcaaatctc cgatccggcc
    11221 cgcgccgacc tcttcggcaa cccgctgccg ctcctgcgcc tccagctcgg cgttggtcgc
    11281 cccgagccgg cggagtgcgc tcaggaactt gttgggcgac atcagccctc ctctggacct
    11341 tccggcagct gcattggcat caacatcccg ggcaccatcg ggctggtccc ccggcgcacc
    11401 accagattac ggaacagttc gacgaaaggc gccaccacgg gtgcgtccgc ctcgccgatc
    11461 gcggcctcgc cgagcagcgt gccccgcagc agccagcgcg ggccctcggc gaaccagacc
    11521 cgggagacgt ggaacatctg ctccccgccg ggcccctcct gcggcagcac gcggcgcacc
    11581 tcggcaccga acggtcccgt tgccagcgtt gcggagccgc cggcctgctc ggcctcctcc
    11641 accgcgtcct cgcggagctc agcggcgagc ccgccactcg cgggcgcggc gaacagcacg
    11701 atctccagac ctgagttctt ccagaccgcg gtggctgcct gcacctgctt ggtggcctcg
    11761 ttcacctgca gctgcaaccc gatgcccggc catggggtga tcaccaccga gccgaggtcg
    11821 atccgggtca cgccatcgcc gcccagctcg acctcttcgg agtcgaacgg gccgtcagcc
    11881 cgccagtcga tctcctcgcc gagatcatcg gcagcgaagt cctcgtcgcc agactcgatg
    11941 tcgtcgtctt ccaactcatc ggtctcggtt gcttcgacct cgtcgtcagc cgggtcgtcg
    12001 gcatcctcgc cgacgatctc cagagcttcc tcctcggtgt ccacggggtc gatgtcggca
    12061 ggcttccgct ttcgcccaaa gatcacttgt tcacctctgt tgtggttcga ccgtccgatc
    12121 ggacggccca ttcagctatt cccccggtag agccgtggcc tcctgagcca cgcacgctgc
    12181 cgggaagttc atcgagcagt tcgaactgcg cggtgtccac ccgctggaac accagttggg
    12241 cgatcaggtc gccccgctgc aaccggacgg gtgccgcagc gtccgtgttc agcaggcaga
    12301 cgcggatctc gccgcgatag cccgagtcca cagttcccgg ggcgttcacg atggtgagcc
    12361 ccgagcgggc cgccagaccg gagcgcgggt gcacgaacgc cgcccagccc tccggcaccg
    12421 cgatggcgat tccggtgcgc accaggcggc gctcccccgg cgcaagttcg cagtcctcgg
    12481 cgatggccag atcggcaccg gcatcgcccg gatgggcata gcgcggtagc ggcagatcag
    12541 ggtcgagcct ggtcaggcca accctcagct cgccagtccc cgccgaagtc attgcggcag
    12601 tctagccgcg ctccgacgcc tctccgtccg gcgagatgga cgcccgggac gccagcgcag
    12661 ccgctagttt ctgcggctcc ctcgtggaca ccaaccagta cggatgcggg tctgccggat
    12721 ccttgatttc cacctcgacc gaacccggca cgtatccccg gacggccagc caggcggcag
    12781 gatcggcctt ggcgcccagg cgctcccgcg tcgccgccgc cgacaaggcg gtcacggcac
    12841 cgagataggg ccactccagc agcgcgtcac cggcgtgcag cccgtccgcg tccaccgcga
    12901 cccgcagccg gccgaaccac agcagtgcca cgcagacggc gatcgcagtc accgccccgg
    12961 cgaccaccgc cacccacggg ccggcgtaga agcccaccgc ggttacgaaa ctgagtgcga
    13021 agaacaggcc gaccagccac cagctcggcg gcacccgtag tcgctcggaa tagtccattg
    13081 ccccaagtta gcttcacttg gcaggatggc attaggagga catggtgaac tcgtcacaga
    13141 agatgctctg gaacctgtac gcggccgcag tcggtgcggc cacgacgatg gtgaccgcca
    13201 aggcactgga atcggcctgg aagctcacca cgggtgatga gccacccctg cccaccgatc
    13261 cgcaggttcc gctgcgccgg gcgttgatct ggactgtcgc cagcgcgctc ggcgtcggcc
    13321 tggcccaggt ggtcgtgaat cgcttcgcgg cgaatcaatg ggtgaagctc accggcacgc
    13381 cggcacccag tgccaagcgc accaacgtaa ccatctgagc gcgccccgtc cgggggggtc
    13441 tcgacaggct cgaccatcga caaactcgac ccctcgacgg gctcgaggaa cgtttcggtc
    13501 agccggcgca gtcgacgcag tagatcagtt gacccttgat ctcggcgatc tggctgcggt
    13561 gcttcaccag gaagcaggaa gcgcaggtga actcgtcggc ctgccggggc aacacccgga
    13621 cggtgagctc ctcgtgtgac agatcggcac cgggcagctc gaaggtctct gcggcctcga
    13681 cctcgtcctc atcgaccttg ccggagttct tgtcgttccg ccgggtcttc agttcctcga
    13741 tcgagtcttc gttttcttca tcgttcttgc gcggggcgtc gtaatcggtc gccatgaggg
    13801 ttgatccatc tccattccgc gatacgcgtc tgcggtttca ctttggtgcc aggaatgcat
    13861 atcacatccg tgctccactt acgtcccttc ccgccacgac gaccaggcat gcggccagtc
    13921 agacgtggtg ttcggtgctc gggttggtag gttacctgca tacacaactg gaagtgaggc
    13981 gatgacccac cagccccagg caccgagtgc tgggagctcg gtgcgcgaac cgccgcagac
    14041 caccctggag attgaaatgg atagcgcgct gaccccaagg gagattcagg cacgcatccg
    14101 cgccggggaa tcggtcgagg aggtcgctcg ggccgccggg atgggcctgg actggatcga
    14161 gcccttcgcc gcgccggtgc tcgccgaaag ggtgttcatc tccggtcagg cacaatcaca
    14221 tccggtgcgg cgcgggtcgg acaccatcgc ccaccgcacg ctcggcgagg tggtcgccga
    14281 ccggctggtc tcccgccggg tggacgtgga cacgctcact tgggacgctt ggcggctcga
    14341 gggtcggcgc tgggtgatcc gcgtcgacta cgactccggg aaggcgcacc gcgaagcccg
    14401 gttcatctac gacgccggta gccggttctc ggttgccgac aacgacgatg cccgctggct
    14461 gctcgggctg cactccgcgt cccacggtcc gcagcccggc cgtcgtcgcc gcgacgaaga
    14521 ggccgagccc accatcgggc tcaacgacga ccttgccctt gtgcgcgtgg tacagccggc
    14581 ggtggacgaa ccggccgatc ccaacgacac cgcacccctg aatgatctgg acgccttcgc
    14641 cgacggtgac gacgccttcg ccgaaggcga gctgaccgag gtcgacgggg tctacgacat
    14701 cgtgcccagt tccgattccg gtctggatct cctttacgac atgctttcga gcttcgacga
    14761 ggactccgtc cagatctacg ccgggttgat ccggcacacc gacgctcccg agcccagccc
    14821 ggtgctggac gagacgccgg tcgccgaacc cgaaccggct gctgaggtgg ctgccgccga
    14881 gcccgcaccc gaactcaccg aaccgcatca accggccctc accgagctcg aagagaccga
    14941 gcccgagccg cagccagcac ccaagcccaa gcgcagcaag cgggcgcagg tgcccagttg
    15001 ggaccagatc atgttcgggt cgcccaagca gcgctgacca gcgccgctca gccgaacggc
    15061 agcggctccg ggctgagcgt gaccgcggcc acctgggcgg tcaccttgcg acgatgatgc
    15121 cgacggcaga gtacttggta ggcgacctcg gcggcatcgt tggtgtcgcc caccaccacc
    15181 tggctgcctt cggtaaccat caccccgttc accgtccgcg cgttgtgcgt ggccggctcg
    15241 ccgcaccaac aacgcggacg cacctgcagc aattccatgc gatcgcacag ctcgaccagt
    15301 cggcgcgacg ccgggaacag ctcggtgcgg aagtcggtga ggatgccgaa acagaacacg
    15361 tcgatctgga gttcgtcgac gatccgggcg agttggtcga tgtgggcagg ctcgaagaac
    15421 tgcgcctcat cgcagacgat gaagtcgatc cggcgtccgg cggtgagttc gccgacgacg
    15481 tgctgccaga aatccaggtc ttcggtgacc tcgtgcgcgg cggcgctgag tccgatgcgg
    15541 gaggtgatgg tcgaggatcc ggcgcgatcc aacatggtga acaggcttcc ctcgcgaccg
    15601 gcgtgcgact cggtgtaggc gacctgcagc gccagggtcg acttgccgca atccatcgga
    15661 ccggtgaagt aggtcagttc agccatggcc gccattctct cctatccagc tgctggtgct
    15721 gacccgcgcg acttcagtcg accagcaggg gaacgtccag ctccgccggg gtcagcgagg
    15781 cgtgcatgcc gaccagggtg agctccttcg gcagcatccg gctcatcacc gcgccgtcgt
    15841 cggccatcgc caccagcacg ttgccgaatc ggtccgccaa ccttggcgaa accgcgccga
    15901 accagccttc ttcgatcgcc tcggctcggg tccgcaccca ggcgctcgtt ccgagccgat
    15961 cccgccagcg cgcagcgacc gcgtccggct ggcgggtcat cagttgccgc agcctgccct
    16021 cgccggcgag cgcggtcacc tcggccagca gatccggctc atcctcgaag aacagtcggg
    16081 acgccggcgg cacgttgatc atgccgtggt cgccggtcac gaccagccgg acatcgtcgg
    16141 gtagggcggc ccgcacctgc gcgagcagtg cgtctgcgga cgccagttcg tgcagccaag
    16201 gcgctgagcc aatgccgtgc tcatgccccg cgtgatcaag ctggcgctca tagaagtagc
    16261 cgaccgcgcg tccgctgccc ccgaccgact gaactgcaag ctccacccgg cgagcagcgt
    16321 cccgttcgtt tgtcaccggc aggaagctgg gcccgcgcac ggccgcggtg gtgagaccgc
    16381 tgccggcgaa gaaggccggc gccaccgtcg cggtccgcac gcctgcggac gcgagtcgtt
    16441 cgaagtaggt cagttgcggc tgcacgtcca ggccgctgag cgttgccggc cacttcaggg
    16501 tgttcagcac catgcgctcg gccgggtacc agaacgagta gccggcaatg ccgtgctggc
    16561 cgggcaccaa cccggtgccg agactggtga tggacgtagc ggtggtgctg ggcacgccgc
    16621 tggtgatgac gcgtcccgac agcccggaga gatagggcgc gtgctcggcg tactcattga
    16681 gttgatggtg gcccagcccg tccacgagag cgatcagata gcgctcagcc ggtggcagtc
    16741 cgagccggtc gtccgccccg ggaacgccga ggtgtgcacc caggctgggg agcaggtcgg
    16801 cgagagtcga gacgccgtag tccggcagcc tgggcggagg catcaatggc tggcggtggc
    16861 cagctgcagt gcagtaccga aggcgaccag ctgggccagt ttggcgttgc cgtcgccggc
    16921 ggaactgatc cggacggtga ggtcgtcgcc ggcgatcatg ccggtcagcc cgtggtcggc
    16981 gtcacagttc ggatcggcgc agtgcgccgg ttccaggtcg acccggcgca tggtgcccca
    17041 ggagatggtc agccaggcct cgacggccga cgacttgtgg gtgccatagc gctcgggatg
    17101 cgcgaccacc tggctcagcg cgaccgagtt cacctgccga aggcccaccg actcggtcgt
    17161 ggtcagtgcc tgtgccggtt ctcctggatt gtcgttctcg tcggtgtggg ccacgatcag
    17221 gcgggtggcg gtcagcacca gcaccgacag gtgccgctgg atctcgtcgt ggttgaacgt
    17281 ggcctcgtgg tgcacgagat gagcggtcgg ctcctcggcg cccaacgcca gcgccaccgc
    17341 atcctcgacg agttccggga agtagccgca ggcgtggatc tcagcgtgca gaccctgcgg
    17401 caggcggatg gtggatcgag ttggcacggc cataatcctc ccacgtcgct cgccccggcc
    17461 caaaagcctg gcggccaggg cgagtgcttc gccgccgggg cgtcgccgct cgtggcgcag
    17521 cgactcggat aatgtgtggt gccgtgagca cccgtccctt catcgccgcc cgggtcgagg
    17581 agttcttcga ccgccgggtc gaggggctga tgcgtgccct cggctggcgc gagaacgtga
    17641 tctgctacac cggatacgga tcgagtgaga gcatccgcgt gctcggacgc gtggtgctcg
    17701 tcccaccctg gtcgcggacc agcctcgggc gcgccaccga ggacctgatg aagcgtcgcg
    17761 gcttccgcaa cttcatcagc gtcccctgcg tccgcccgca ggccatgatc gtcgtgggcg
    17821 agcacgagta cccggtggtg gcggaccggg gtggctacat cgaccaccgc gtccgcgagc
    17881 acggcctggt cggcggctgg cagcaggtcg gcgtccgctc agcaacctcg ccgaccgcta
    17941 cggcggacgt gcacgtggtc ggcgacgaag tgaccttcgg catcgtctcc gacatcgacg
    18001 acacgatcat ctccaccatg ctcccccggc cgatgatcgc cgcctggaac tctttcatcc
    18061 gggacgaatc cgcgcgccaa gccgtccccg gcatggccca gctctacgac cggctgctgg
    18121 ccgaacaccc gggcagccca gtgatctacg tctccaccgg cgcctggaac acccacggct
    18181 ttctgggccg tttcctgaag cggcacgcgt tccctcccgg cgcgatgatc ctcaccgact
    18241 ggggtccgac caacaccggc tggttccgct caggcatggc gcacaaggcc gagaccctgc
    18301 tgctgctcgc ccgggatttc cccaatatct cctggctgct cgtcggcgac gatggccagc
    18361 acgaccctgc gatctactcg aacttcgccc gtgtagcgcc tgaacgggtg cgtgcgatcg
    18421 cgatccggca gctcactcca gctgaacagg tgctggccca cggcaccccg tccatgctgc
    18481 cagaggaaga actcaccgaa ccggccgaca cccccaccgt ggcggccccg gatggcaggg
    18541 gcctgctggc cgcgctccgc ggcctcgccg agccgctcgc ctgactgact gaaagcgaga
    18601 agtcccaagc agatggttgc caagccgccg ccagcagaag aagagctgac cgaacgcatc
    18661 gtcgacgtcg acgtcaccga cgagatggaa gccagcttcc tcgagtacgc ctactcggtg
    18721 atctactccc gcgcgttgcc cgacgcaagg gacggcctca agcccgtgca gcgtcgcatt
    18781 ctctacacga tggaccagat gcggatccgt ccggacgcat cccacgtcaa gtgcgcccgc
    18841 gtggtcggcc aggtgatggg ccagctgcac ccccacggcg acgcggcgat ctacgacgcg
    18901 ctcgtgcgcc tcggccagcc gtggtcgatg cgcctgccca ccgtggacgg ccacggcaac
    18961 ttcggctcgc tggactcggg ccctgcggcg atgcggtaca cggaatgtcg catgtccccg
    19021 gccgccgcag ccatgaccgc cgacctggac tcggacaccg tcgatttccg gcccaactac
    19081 gacggcaagg agaccgaacc ggttgtgctg ccggccgcct tcccgcacct gctggtcaac
    19141 ggcagcaccg gtatcgcggt cggcatggcc acgaacatcc cgccgcacaa cctggttgag
    19201 gtcgtccagg cgttgaagca cctgatgtgg catcccgatg ccgacgtcga ggagttgatg
    19261 cgtttcgttc cgggccccga cttcccgacc ggcggcaaga tcatcggcct ggacggaatc
    19321 cgcgacgcct acaccggcgg acggggcgtc ttccgcgtcc gcgcgtcggc ccgcgtcgag
    19381 caggtgcacg cgcgccgcaa gggcatcgtg gtcaccgaac tgccctacca gatcggcccc
    19441 gagcggatca tcgagcaggt gaagaacctg gtcagctcca aaaagctcac gggcatctcc
    19501 gacatcaagg acctcaccga cctaaccaac ggcacccggc tggtgatcga ggtcaagaac
    19561 ggcttcaacc cggaagcgct cctggaggag ctgtaccggc tgaccaagtt ggaggactcg
    19621 ttcggcatca acgcggtcgc cctggtcgaa ggccagccga acacgctcag cctcaagcag
    19681 atgctggagg tctacctcga tcaccgactg caggtggtgc gccgccgttc cgccttccgg
    19741 ctcggtaagg cccgcgaccg gctgcacctg gtggaggggc tgctgctggc gatcgtcgac
    19801 atcgacgacg tgatcgcgat tatccgctcc tcggacgacg ccacggcggc gcgaacccgc
    19861 ctgatggagg ccttcgaact ctccgagttg caggccaact acattctcga gatgcggcta
    19921 cgccggctga cgaaattctc caagcttgag ttggattccg agcgtgacga gctctggctg
    19981 accatcaacg aactccaggc gatcctcgat tcggaacagc ggctgcgcga agtcgccgcc
    20041 agcgaactgg acgaggtggc tcgcacccac ggcacgccgc gacggacagt gctactcgcg
    20101 tccgcgggca cccaggtcac cgcccagtcg gcggcgaacc ggctggagat cgccgacgat
    20161 ccttgctggg tgatgctgtc cagcgccggg ctgctggcgc gcaccgacaa cgacgagccg
    20221 ctgccgacct ccgggccgcg cgccaaccac gacgtgatct cctcggcagt ccggacgacc
    20281 gcgcgcggcg agtactgcgc gttcacgaac cgaggccggc tgatccgcgg ccaggcgatc
    20341 gatgtgccgg cggtgccggt gaccgcagcc gcacccaacc tgcagggcgg ctcgctggcg
    20401 tccgaactgc tgccgctgga cccgggcgag acgatcctcg cgctcggcac cctgggtgcg
    20461 gacaccttcg gctgggcatt ggggacgcaa cgcggcgtgg tcaagcgggt gaatcccgag
    20521 gtactgggca aggactcgtg ggaggtcgtc cgcctggacg acggcgacga ggtggtcggc
    20581 gtggtcgaac tcagccatga cctggtcgag ctggttttca tcacttcgga cgcgcaactg
    20641 ctgcacttcc ccgcgaccag cgtgcggccg cagggacgga cgggcggcgg catggccggc
    20701 gtcaaattgt cccgcggcgc gaaggtggtc ttcttcgggg tcacgccgac agcggattcg
    20761 tcggtggtca cggtcgcagg ctcgtccgca gcgctccccg ggacggaagc cggaacagtc
    20821 aaggtcaccc cgctggccga gtaccccggc aagggccgcg cgaccggcgg cgtccgctgt
    20881 caccgcttcc tgaagggcga ggacaccctg ctgctcgcgt gggccgggcc cgacccggtg
    20941 atcgcagccg cggccagcgg taccccgatc gacctgcccg cacccgatcc gaggcgcgac
    21001 ggttccggcg ccccggtggc ccagccggtc gccgcgatcg gctcccgcta ccacgcctga
    21061 cccgggtctc gtccggccgg tcagcccgtc cacgcccacg tttgctaccc aaatggtcgg
    21121 gagtctcggc tgtcgggagt ctcggctggt cgaggttgtc gagaccccga gacagtctgc
    21181 tcagtactct gagcccgtgg acaactcttt cgacgacctg ctcgcggcca acagccgcta
    21241 cgccacctca gcgccccgcg gcttcgacgg ctatgcccac gccggcgtag ccgtggtcac
    21301 ttgcatggac tcccggctgc agccgctgga gatgatcgga ctcttccttg gcgaagcgaa
    21361 gatcctgcgg acgccgggcg ggcacgtcac cccggacgcg ctggccggct gcgtgctcgg
    21421 cgtgaacctg ttgcaggtga accgcatcct ggtgatcacc cacagccgct gcgcgatggc
    21481 gtccggggac gacgaggaga tgcgtcgccg cgtggccgcg gccagcggtg ccgacacctc
    21541 gggctacagc ttcggcgccg atcccgacca gttcggacgc ctccgagcgg acgtcgccgt
    21601 tctgaccgag cacccgctga tcgccggcca cgccaaggtg ggtggcttcc actacgatgt
    21661 ggactcggga ctcctcaccc aggtctgctg acacgaggtg aacactccag ccaccgtccc
    21721 ccggaagccc cggctggccg tcggcggctt caacctgctg gttcacggct tcgggaagta
    21781 ggttcagacc gggctgtaca cggtcgagtg gacgatgtgg tggataccca gcgtcttcgc
    21841 gccgatctcg ctggttgcca cctggcgtgg cgccatctac tcctgcgtcg ggtacctggt
    21901 ggggctgctc gctggtgagc tgatcgggga gccggtctac caagcccagc tggcacgtcc
    21961 gcatgagcag aagcaggacc ccagctacat ccagaactgg gagccccatc accctggctg
    22021 gtagatctgc atcctggtct gcttggcgtt ctcgatcgtc ggcctggtgc tcggcctccg
    22081 agccgagaag cgggctcagg cgtcgatgcg ttccgcgtcc aacgcgtagg caccctcgat
    22141 gatgaactcc ttgcggggag cgacatcgtt acccatcagc ttctcgaaca ccgcatcggc
    22201 cgcacctgcc tcatccacgg tgatccggcg cagcgtgcgg tgccgtggat tcatcgtggt
    22261 atcagccaac tgatcggcgt ccatctcgcc cagccccttg tagcgctgcg gctccttgaa
    22321 cggctggccc ttcttggtga gctctgccag cttgcggcgg tattcggcct cggagtaggt
    22381 gtagatgtac ttctcctgac ccttgcgcgg gttgatcatc tcgaaccggt gcagcggcgg
    22441 caccgcggtg tagacccggc ccgcttcgac cagcggacgc atgtacttga agaacagcgt
    22501 ggccagcagg gtgcggatgt gggcgccgtc ggagtccgcg tccgccatga agatcacctt
    22561 cgagtagcgc gctgcgtcga ggtcgaagct gcgccccgac cccgctccga ccacctggat
    22621 gatcgaggcg cattccacgt tcttcagcat gtcgccgacc gaggccttct ggacgttcag
    22681 gatcttgccg cggatcggca gcaacgcctg gaactccgaa tcccgggcca gtttggccgt
    22741 gcccagcgcg gagttgccct ccacgatgaa cagctcggtg cggtccagat cggtgctgcg
    22801 gcagtcggcc agcttggccg gcagcgtgga cgactccagc gcgttcttgc gccgctgcgc
    22861 ttccttgtgc gccctcgcct gcacccgcgt ccgcgaagcc gcgaccacct tctccatgac
    22921 cgctcgggcc tgggtgcgca acgcggcctt ggtggagttc aggaaagcgc ccagctcgtt
    22981 ctccacgatc ctggcaacca gccgggtgac cggcggggtg cccagcacct ccttggtctg
    23041 gccctcgaac tgcggttcgg ccagccggac ggtgaccacg gcggtcatcc cttccaatac
    23101 gtcgtccttg gtcacctcct ccccagcctt cagcagccgg gtgccctcga agcccttcgc
    23161 gaaagccctg gtcaggccac gttcgaaccc gctgacgtgg gtaccgccct tgggcgtggc
    23221 gatgatgttc acgaatgact ggacggtgtg gtcgtagcca ttgccccagc gcagggcgat
    23281 gtccacgtca agcgtgcgct ccacgtccgt cggcgtcatc gcaccggcgt cgtcgagcat
    23341 cggtacggtc tcggtgaact ggtcggtgcc ctgcagccgg acgatgtcgg tcacggccgc
    23401 gtcgcgggcc aggaagtcac agaactccga gatgccgccg tcgtgcttga acgtctcggt
    23461 ggccgcctca cccgaacggg cgtcggtgac cgtcagctca agcccgggca ccaggaaact
    23521 ggtctgccgg gccctggaca gcagttggtt gagggacaac tccgcgttcg gcaggaagat
    23581 ctgccggtcg ggccagtaac gcacgcgggt gccagtcacg cccttcgccg cccggccgac
    23641 cttacgcaag gtgccgccgg gagtgaacgg cgcgttcggt ccgtccccgt cgaagacccc
    23701 gaccacgccc cggcggaagc tcatcgcgta ggtggcaccg ttgcgatcga cctcgacgtc
    23761 gagccgagaa ctcaacgcgt tgaccacgga cgcgcccacg ccgtgcagcc cgccggtggc
    23821 gttgtagctg cctgcgccga actttccgcc ggcgtgcagc ttggtgaaga ccacctcgac
    23881 accggtgagc ttggtgcgcg gttcgatgtc gaccgggatg ccacgggcac ggtcgaccac
    23941 gaggatgctg ccgtccgccg cgagctgaac gtcgatgcgg ttgccgaagc cggccagcgc
    24001 ctcgtccacc gcgttgtcga tgatctccca cagacagtgc atcaggccgc gggtgtcggt
    24061 cgagccgatg tacatggccg gacgcttccg cacggcctcc agcccctcga gcaccagcag
    24121 gtgcctggcc tcgtagtccc tgccctcagc cggcttggtc gtgttcacgc aggcgaggct
    24181 atctcccccc ggtgacagtt ctacgcattg gggtcggtgt cggtcgtcga ggactgtctg
    24241 ccgtaccacc tacactgtga gcaccgggaa caacttgggt aagcatgttg ttgagaccgg
    24301 cgaatgctcg ccagctgaag atgtgggtga gagagggagg atgaccgatg agtgccactg
    24361 ttaccgaacc gttgaccgct gccgatcgtt gtgaccgctg cggtgcccaa gcctaccttc
    24421 gcgtaaccct caactcaggc ggcgaactgc tcttctgcgg ccaccacgga cgggcccact
    24481 cagcgaagct caaccaggtc gccctgaaga tccacgacga aacggcgaag ctgtcctgat
    24541 tcagaccacc aagtggcccc gggagcttcc cggggccact tctgtattcg ggctgaaata
    24601 ctgccgcaca tgaccatcga cgccgttcgg ggcgatctca cccgtgagcc ggccgacgtg
    24661 ctggtgaacg cggccaacgc cagtctgctc ggcggtggtg gcgtggacgg cgcgctgcat
    24721 gccgccgccg gaccggagct gctggccgcc tgccgcaagg ttcgcgccac cagttggccg
    24781 gacggcctgc cggtcggcga ggcgatagcc accaccgccg gactcctacc cgcactttgg
    24841 gtagtacaca ccgtggcgcc gaaccggcac gccgggcaga ccgacgaggc cctcctggcc
    24901 agcgcctatc gccgcagcct ggacgtggcc tgcgacctcg gcgcgcggac ggtcgccttc
    24961 ccagctctcg gtgccggagc gtacggctgg gacgcggcga ctgccgcccg ggtagccgtc
    25021 caggccgtgg ccgcgcatcc acgcacgggt ctggagcgcg tgcgtttcgt gctctacaac
    25081 gaacctctac atgcggcctt cgttgccgag ttggcgaggc tggcatgacc gcgatgccct
    25141 acggctcgtg gccgtcgccg attctggcgg ccgacctcac tcgctcctcg gtacgtctct
    25201 cgcccgggcg gatggacgcc ggcgtccgct actggaccga aggacatccc gagcagggcg
    25261 gacgggtcgg cctgtggcgc cagggaccga acgagccccc gaccgagctg acgccggagg
    25321 cctatgtacg caccggagtc aacgagtacg gcggcggtga ctggaccgtc caggacggcg
    25381 tggtggcgta ctcttcgtgg ccggacggca gcgtgcaggt gcgtacgaac gacggtgcca
    25441 ccacagagct ggcgcccggt gggcggttcc ggtacgccgc gctgagcctg caccgagagt
    25501 tcgggctgct gcttgccgtg cgagaagacc acgaggttgc cggcaacgag ccggagacca
    25561 ccatcgtcgc gctcgacctc gccgaaacca acccggacgg cggacgcgtg ctggtcgccg
    25621 gcgccgactt ctacgcccat ccctgcctgg acgcgtccgg acgcctggcc tggtgcgagt
    25681 gggaccaccc gaacctgccc tgggactcga cccggatcgt ggtcgcaccg ctggactccc
    25741 ccgctgaccg gacgcaggtc gcgggcggcc ccggcatctc ggcgctgtac ccggcgtggg
    25801 cgccggatgg agcgctgctc tacctcagcg atgccagcgg gtactggaac ttcgagcgct
    25861 gggacggtag cgccagccgt ccgctgttct cggcgcctta cgacttctgc ggcccgctgt
    25921 gggtgctcac gccggtgccg tacaccgtga tcgacgccag ccggatcggc tgcacctggt
    25981 tggcggacgg cgtcgcgagg ctcggcgtac tggccttcgc tgaccccggt tacgccgtcg
    26041 tcccggcaaa caccgtcatc ccggcgaagg ccgggatctc ccagcgcgat cggtggatct
    26101 ctgctgaaga ccggcccggc accctgaccg agctcggtgt gactgccgtg gcagccgacg
    26161 tcaccggtac cggcgtcagc actctggcag tgctgggcta cgccgatcgt ccggccgagc
    26221 tggtcgaact ggattggatc accggccaaa cgacggtggt gcggaaggcg tccgaagtcg
    26281 acctggacca ggcgatgatc tcggtggcgc agccgttcag ttgggagtca ccggacggcc
    26341 ccgtccacgc ctggtactac ccaccgaccc atgccgacgt gacggcgccg gccggcgaac
    26401 tgcccccgat ccaggtctgg agtcatggcg gtccgaccgc gttcagcgac cccggtttcg
    26461 cgctcgccgt ccagtactgg acgactcgcg gcatcggcat tctcgacgtg aactactccg
    26521 gctccaccgg ttacggccgg gcctaccgcg atcggctgcg gggcacatgg ggagtcgcgg
    26581 acgtgcgcga ctgcatcaac ggcgcggtcg cgctggctgc ggccggtctg gccgatggca
    26641 agcggctgag tatccgcggc gggtcggcgg gcgggttcac gaccctggcc gcgctcacca
    26701 cgagcaaggt cttcgcttcg ggcatcagcc tgtacggcat cggcgacctc gaaaccctcg
    26761 ccacagatac ccacaagttc gagaagcact acctggacgg cctggtcgcg ccctatccgg
    26821 cgggtcggca gacctacctc gatcgctccc ccatccgcca tctcgaccag ctgagctgcc
    26881 cgatgctgct gctccagggc agcgaggaca aagtggtgcc gccgagccag gccgaagcga
    26941 tggcccaagc ggtcctggcc aaaggcctga ccgccgacct ggtggtcttc gaaggcgagg
    27001 gccacggctt ccgccgatcc gagaacatca tcaaggtggc cgaactagcg ctggccttcc
    27061 tcggccgagt tcacggcttc aggccagcca cctgaggatc cctgagccct cagcaaggac
    27121 cgtccgggag tctgtggagc tccgcgccgg ccagctgccg atcgaagttc agcagttcaa
    27181 ggctgagcag ctgcgaacgg tcagcgagaa ccagtcggtt cccgagatct ccaggtccat
    27241 cgcacgatag tagggtgacc gccgtggtta cgcatgtggt ttgggactgg aacgggactc
    27301 tgctcgacga cctgcactgc tgcatcgacg tgaccaacac gctgctcacc gagtacgggc
    27361 tgccggtgtt ggacgacgtc gccgcttatc agcgggtctt ccggttcccg gtggccgact
    27421 actacgccga tctgggcttc gacaccaggc cgggcgggaa tttcgacact gcgtccgtcc
    27481 gctacctgga gctgtaccac gccgccgccg ccggctgtga actccacaac ggtgcgaggg
    27541 agaccctgcg gacgctgcac gcggccggcg tccgtcaggt ggtgatctcg gcctcggagc
    27601 agcagaacct gctcaagcag ctgtccccct tcgagctgaa cggctggctt gacggagccc
    27661 acggcatcgc cgacatctac gccgcatcca agctcagcat cgcccagcgc tggctcgcga
    27721 cgaccggagc tgaccccgcc aaagtggtct tcgtcggtga ctccgaacac gactacgaga
    27781 tcgcgatggc gctcggcgcc tgctgcgtcc tgttcagcgg cggccaccac tccagggacc
    27841 atctacagac cctggacgca cccgtggtcg atgacctgac ccaggtcgca gcgcacgtcc
    27901 gctagttctc tggcggtcga gcatgtcgaa accgcccaaa ccggctgcct ccagacaaag
    27961 gacgtgctca cctcacagga ttccaactgc gtcttgatga ccagagcagg gccccagcct
    28021 cgctattgtg agttgagctc gtcgggccgg cactcgccca tgctgaacct gcgggtgtcg
    28081 gaggacacca aagcgcaact ggagcacctg gccgaagcca gcggccgccg gcagtccgat
    28141 ctcgtccggg acgcactggc cgagtacctc gaacggcacg cttcctgacc tcaccgggac
    28201 tcaccccggc tgaacagctc ccatccgcgc aggaggagcc gagccgagaa gaagggcgcg
    28261 catccagctg gtggcgggag catcaccgcc acctcgacga ttgccaaaga aaccgctgat
    28321 caatgcggcc caacgctggc tgagcttgac taagcccgta gccaaaagcg aatggccgac
    28381 ccaatgcccg gctcagggat cgttgatcag cgcttgccta aggcatcagg taccgcgccg
    28441 agatgatcag tccaggtagt cgcgcagcac ctgcgagcgg gacgggtgcc gcagcttgct
    28501 catggtcttc gactcgatct gccggatccg ctcgcgggtg accccgtaga ccttgccgat
    28561 ctcgtcgagc gtcttcggct ggccgtcggt caagccgaag cgcatcgaga ccacgccggc
    28621 ttcgcgctcg ctgagcgtgt ccagcacgtc gtgcagctgt tcctgcagca gggtgaagtt
    28681 gaccgcctcg gccggtacga ccgcttcaga gtcctcgatc aagtcgccga actcggagtc
    28741 accgtcctca cccagcgggg tgtgcagcga aatcggctcg cggccgtact tctgcacctc
    28801 gacgaccttc tccggggtca tgtcgagctc cttggcgagc tcctccgggg ttggttcccg
    28861 gccgagatcc tgcagcatct gccgctggac gcgcgcgagt ttgttgatca cctcgaccat
    28921 gtgcaccggg atgcggatcg tgcgggcctg gtcggccatg gcccgggtga tcgcctgctt
    28981 gatccaccag gtggcgtacg tggagaactt gtagcccttg gtgtagtcga acttctcgac
    29041 cgcacggatc aggcccaggt tgccctcctg gatcaggtcg aggaagagca tgccgcggcc
    29101 ggtgtagcgc ttggccagcg acaccaccag gcgcaggttc gcctccagca ggtggttctt
    29161 agcacggcgg ccgtcctcgg agatccagct gaggtcctcg gacgggcccg cgcccgtacc
    29221 gccctcatgc agcgtctcct cggcgaacag accggcctcg atgcgtttcg cgagctgcac
    29281 ctcctgctcg gcgttcagca gcgcgacctt gccgatttgc ttcaggtagt ccttgaccgg
    29341 gtcggcggtg gcaccggcca ccagcacttg ctgttccggc tcgtccgcgt cgtcggcgtc
    29401 agaaaggctg aagccaccgt cctcggccag ctcctgctcg gtcttggccg cttcggccgg
    29461 ctcgaacgcg gactcgtcca cgtcatcgag ggtgcgcttc ttaccgccga cggtgagcac
    29521 cacgtcgttc ccctcccgct cgaacttcac gttctcgatg ttggcgggag ccagcacgtc
    29581 ctctacggtg accgcgtcga gttcaacctc ggcgtcgtcg atggtgtcat cggtgggctc
    29641 caggtcctcg tccggaccgt ccaccgcagt cggctcaagg ttcggcagcg cggttaccgg
    29701 ggccggggct ttccgcgtcc ggcgcttggg agcggtggtg gccgcctctt cgttggcggg
    29761 gacagcggct gcgctggagc gtggagacaa aggcttaacc tctcaacggg cgattcaggg
    29821 cgcacagcaa ccgggagttg tcaatgcttc gccgttccat tctgccacgc cggacgccaa
    29881 cacccaagcc ggcgccaggt gcgcggcgtg aggacttcgg cgaacgaggg gcaacctgcc
    29941 tacaaccacc gacgacccta ggctgttccc catgtcgcag ccaccgctgg ccccccgcag
    30001 gccgagccct cgtcgtcatc acggcgacac cttcgtcgac cactacgcct ggatgcgcga
    30061 caagtccgac ccggccttcc tggcctacct ggaggcggag aacgcctaca ccgaggccga
    30121 gaccgcaggg ctcgccgacc tgcgccagga gatcttcgag gacatctcgg ctcgcaccaa
    30181 gcagaccgac ttgagcgtgc cggagttcgt ccggcaccgc ggtctgggag atttctggta
    30241 ttacgcccgc tcaaccgaag gccacgacta ccccagctac caccgctgcc cggcgcaggg
    30301 acgcgactcc ttgccccacc cacaagccgg aagtccggtc ggcgagcagc tgctgctgga
    30361 cgcgcaggtg cttgccgcgg attccgcgtt cttcgctctt ggcaccttca acgtctcccc
    30421 ggacggacgg ctcgcggcat actcggtgga ccacagtggc gacgagcgct accgactggt
    30481 gttcctcgac ctgaccaccg gcgaactgct gtcggacgaa ctgcccgagg tggcggccgg
    30541 tggcacctgg gccggcaatg atgccttctg ttatctgacc gtcgacgagg cctggcgacc
    30601 ggagaaggtc tggcggcacc ggctgggcgt caccggaccg gacgagttgc tcttcaccga
    30661 gtccgatgaa cgcttctggc tcggcgtgga cagctcgcgc gacgaccgct ggctcctggt
    30721 ggcatcggcc agcaagacca ccaccgaagc ctggctgctc gacctcgtcg atccggacgc
    30781 ggaactgcgc tgcgtccggc cccgcgtcga ggggctcgag tacgacgtcg aggtcgccag
    30841 cgaccggctg ttcatcgtcc acaaccagga cgccccggac ttcgcgctcg cgcaggcgcc
    30901 actgaccgcg gccaccggcg ctgactggac gccaatctgg gccggcgagc ccggcgtccg
    30961 gttgctcggt gtgaccgcct acgaccgggt gctggtggcc agcgtccgcc gcgacgggct
    31021 gcaactggtc acgttgcgct accgggacgc caacggcgat gtgggtcctg aaaccgcgat
    31081 cgcattcgac gagccgctct accaactgga tgccgatgac ggcgacgagg ccgacaccga
    31141 ccggatccgg ctgcactacc agtcgatggt cacccccgac caggtgatcg agtaccgact
    31201 caccgacggg gcccggaagg tactgcggca gcgtccggtg ctcgaccacc ccaggttcgg
    31261 ggcttacgac ccgtccgcct acgtccagcg ccgcgagtgg gcaaccgcac cggacggcac
    31321 ccgggtgccg atctctctgg tacaccgcgc agatacccca ctcgacgggg cggccggctg
    31381 cctgctctac ggctacggcg cctacgagat cagcatcccg ccgacgttct cgatcgctcg
    31441 gctgagcctg ctggatcgtg gctacgtcta tgcgatcgcc cacgtccgtg gcggtggcga
    31501 actcggccgc gcctggtacg acggcggccg gctcgcggcg aagacgaaca ctttcacgga
    31561 cttcatcgcc tgcgcccggc acctggtggc agcgggctac acgtccgccg atcggctggc
    31621 tgcggaaggc ggcagcgccg gcgggctgct gatcggggcc gcgatcaacc tcgccccgga
    31681 caccttcgcc gcggtgcacg cagccgtgcc gttcgtggac gcgctgacca cgatcctcga
    31741 tccggagcta ccgctgaccg tgatggagtg ggaggagtgg ggcgatccgc tgcacgatcc
    31801 cgacgtgtac cgacgaatgc gcgggtattc gccctacgag aacatccgga cgggtgagta
    31861 tccggcgatt ctggtgaccg ccagcctgaa cgacacccgg gtcgaaatca ccgagccggc
    31921 caagtgggtc gctgaactgc gacacacgtc cggctcgacc cggccgattc acctgcgcac
    31981 cgagctgacc gccggccatg gcggtaattc gggtcgctac caggggtggc gggacgctgc
    32041 gttcgaattg gcgtggctga tggcgcacac ctcccagggt taggggtatg cgtacgacca
    32101 cggtcttgat ctgtccccta gggatgcatt cagggtagct accggggcgt cgcgggcaga
    32161 aaaccaggcc gctctcgcgt tgtactgatg caagagcgaa agggacgtac gtgatagcta
    32221 ccgcacaccg gatccgcagc accgacggga tcgacggcaa ggacgcagtt ggcctatacc
    32281 ttgacggaat cgccaagact ccgctgctga ctgctgaaga ggaagtccag ctggccaagt
    32341 tgatcgaggc cggacggtac gcccacgcga ttctcgacgg caccctcgac gatgccgagt
    32401 cggccctttg ccgcgtccgc gccaccgagg acgaactgac cgagatcgcc cgccagggcg
    32461 acgccgcgat gcagcgcttc atcaccgcca acctgaggct ggtggtctcc gtggcccgca
    32521 agtacggccg cgcccagatg ccgctactcg acctggtgca ggagggcaac accggcctga
    32581 tccgcgcggt cgagaagttc gactacacca agggcttcaa gttctcgacc tacgccacct
    32641 ggtgggtgcg gcagtcgatc tcccgcggca ttgcgcagca gggccggatc gtgcgccttc
    32701 ccgtgcacgt cgccgagcag gtcaaccagg tctcagccgt gcgccggacg ctcgaacgcc
    32761 gctggggacg cgaacccgag ctggtcgaga tcgccgacga actcggtttg accgaggagc
    32821 aggtgatcga tctgctgcgt ctctcccgtg accatgtcag cctggacgca ccggtcgagg
    32881 aggacggcga caccgcgctg ggcgatctgc tggcccgcga gatgtccccc ggcccggacg
    32941 aggtcgtgct ggacgccgag gatcgtgctc gtctggaagc catgctggcc accttggacg
    33001 accgctcggc cgacgtcgtg cgccgccgct acggcctgct ggacggccgc caggccaagc
    33061 tcgccgacat cgcggccgtg tggggcatca ccgccgaacg cgtccgccag atcgaacggc
    33121 acgccatctc caagcttcgc ggggacgccg aagccgccta accactggct ccccaacaac
    33181 ggtccctgag gagcgctggc gagcttgcga gcagcgcgtc acgaaggccg cccgaggggt
    33241 gaccagacct aactactgct cgggtcgacg attcggatca ggccctcctg ggcgcaggac
    33301 gcggccaggc ggccgtccgc gaacaaccgg ccgaagctga acccaagccc cgcggacgca
    33361 gacggcgaga cctggtcata gagcagccac tggtcggcac gggcgggccg gtggaaccac
    33421 atcgagtggt tgatggaagc cacctgcatg ttcggcccgc cgaactcgac cgggtgcggc
    33481 actgtgctca cggcgagcag ggtcaggtcg gaagcgtagg ccagtaccgc ctggtggatc
    33541 tgtgggtcgt ccggcagcgc gctctccgcc cgcacccaga ccttcatgtc cgcgtccgca
    33601 tgagcggctg ccgtcgccac cgaagtgtcc gaagcgaacc gggtctccag gacgccccat
    33661 tcctgccgcc aggccgccac cgcgcgcctc gaacgggcac cgagcacctc cgacaagggc
    33721 ggacagtcct ccggcggcgc cacaccgagc ggcgcctggt cggcgtggtc cagaccagcc
    33781 tcaagctcgt ggtacgaaca caccatcgag aagatcaccc ggccgtcctg cgcggccacg
    33841 acgcggcgag ccgagaagct gccaccgtcg cgggtcttct ccaccgaata ggtgatcggc
    33901 actccgggag cacccgggcg caggaagtac gctcccagcg agtgcgccac ccgtccggcc
    33961 ggtacggtgc ggtacccggc ggtcaaggcc tgcgcgagca cctgcccgcc gaagacccgc
    34021 tggaacatac ggccttcctg gtcatcgtcc tgaccgagga aagtggcctc gccggtctgc
    34081 tccaggtcga gcagggcaat cagttcgtcg acgctctctg gcaaggtcag cgaccgttcg
    34141 gcggatcctg ctcggcgagg aactgctcca ccgccgcgcc gatctcgtcg gcactgggca
    34201 tggaggccgc accgctgctg ctcagccggt cgtactgctc ctccagtgtg gcgaccagcg
    34261 ccgggaactc ggcgtcctcc gcggtctcgg acgcgatcga ggcgaggttc gccgcggcgc
    34321 gctcggccag cgcaagcatc gggatctcca agccggccgc gtccgccagc cgctgcacga
    34381 ccgcctcggt ggcctgcggg aagctggtct gcgcgaggta gtgcggcacg tgggcgacga
    34441 agccgccggc caggatgccg gcctccccag ccttgaactc cagcatgtgc gagaagctgc
    34501 ccggaatctg gacgcggtcg atccacatcg gattgccgct cactgtctcg ggctgcgtgg
    34561 catgcgcggt caccagggtc ggacgcgtgt gcggcaccgc catcggcacc ccggagacgg
    34621 tcagcatccg ggaaacgccg agctgttcgg caatgccgag caccgcgctg gctgtccggt
    34681 tccattgggt gtccggctcc ggccccgaca gcatcagaaa gggcttgccg accgcgtcct
    34741 tgagcttgtg gatcaccagt gcgtaatcgg tcatccccac ccagtggtcg gtgtcgaaga
    34801 ccatctgtgg gcggcgggag cgatagtcgt ggagctgatc gtggtcgaac tcgaccagta
    34861 cctcgtgctc gcacacttcc agcaggtagc ggccggtgcc gtgggtgatc gacccggcgt
    34921 cgatgtaccc gtccagaagg tggatgagtg caggctcggc cccccggacc gaatcccaaa
    34981 gctccgggtc gagtgagtac agcaaccggg gatctagcac gcggccactg taccttgcac
    35041 cttcggccgg tggccggatt gcgctgtggg ctaactgggg ctaactgtcg tgggtgtccg
    35101 gttcggcggc gctcgtgggc gcgacgatcg gctgcagctc gcgaatccgt ccgcccaggt
    35161 cggtgagtag ttcgatcgtg cgcgccgctg ccgggaacga ggcggcgccg ttgcgcagca
    35221 gcgccacgat tcgccggtac ggcgtcgggt tggtcggccg aacgtacgcg atctcgaagc
    35281 ccgaccagcc gacggccagt tgcggcacca gcgcgatccc gaccccggcc gcgacgaagg
    35341 cgagcccggt gacgtggtcc tgcgcacgca ccacatagcg cggcgtgaag ccggcaccgg
    35401 cgcaggcatg gctggccaga ctggtcgcga tgtcgtcgcg gaagtcgtag tgcacccagt
    35461 catcggtgct gaactcggct agatccatcg tcccggcctc ggccagcggg tggtccggcg
    35521 gcatggccac cagcaggtca tcggtgccca gttcgatgcg gcgatagccc ggcgggaccc
    35581 tcggctcctc gtagggcagc tcagtgtgta cttcgacgtc cgcctgaccg gccaactccg
    35641 ggctgtcggt ttcgttgatc gacagctcga gcgtgagccc ggggaattcc tggcgcagcg
    35701 tccgggcgac cgtcggcatc caggtgtagg ccgccgatgc gaaggttcgc atggtcagct
    35761 tggccgtggc accctgccgc aactcccgcg ctttggcgtc caccttggtc atcgcggcca
    35821 gtgcatcctg gctggtcagc gccagttcga tggcggccgg cgtgggctgg atgccccgac
    35881 cgacccgctc caccagggtg aacccgacct cgcgttcgag ggtgtggatg tgctggctga
    35941 ccgtcgacgg ggtgaggccg agccggcggg cggtgtcgtt gaccgagccg ctggcgacca
    36001 ccgagcgaag aatccggagt cgattgagat cgagcacggc agaatcatac ggcatcaccg
    36061 catgattgtt cggaatattc agcttgtccc gaatgaacga gtgcgagaac cttgagccat
    36121 gttgagctat gcagtgaccg accgcgaccg cgagcgcctg atcgaggacc tcgaactcgc
    36181 gcgccagatc gaacccacgc cgatggtgcg gcggaaggtg gacaagcgcc actggtttcg
    36241 tatcgcgatg accgagggcg agaccatccg cacccacttc gcaagcgtct gatcctcacc
    36301 gagcggctgc ggccatcgcc gtccgcagcc gcttgggcga gaccgggact gatgttcgca
    36361 gcgcttgcgc gaacaggctc acccgtagtt cctccaacaa ccaacccacc tcagccaccg
    36421 gctcgggcaa cggcccggac gggaatcccg cgcagagtag cgcgtactcc tcttccagct
    36481 ccgtgatcgt cgcgaggccc tcggcctcgt gccgcggatt gctggctgcc gcgaccaatc
    36541 ggcgcccgac cgcggccaga taggtgggca ggcggtgcca ccatttcggc gggatcgccg
    36601 cgacgaagcc agcgaacacc agaccgttga gctgctcggc gacgtcctgc cgcgtgcggt
    36661 cggtcgcggt cggcaactgc tggcgaatct cccccgccgc cacaaggatc tcgccggtgg
    36721 cctgcacgac ctgctgcatc gtggcggccg cctgggcacg gaccgcgtcc gccagtcggt
    36781 tgaacgcagc ctcgtcgcgc acctggtcca gcccgccgac cgcgtccatc aggtcaccga
    36841 tggcgcgcaa cctggcgtcg gcgagcagcg cgggcacgtc cggatagggc gaagtggcca
    36901 gcgcgagctt ggtggggttg ctcatccgcg agaccaccca gcgggtcggg tcgaccgtgg
    36961 caagggagat cagccggcgc aggccggcgc ggtgggcgcg gcgcgcgtcc gcctcatctg
    37021 cgaacacctg gacgcccacc tgctgcaccc ggtcgaccag cgcagggtag ccggtcagct
    37081 catcgcccag tcgctgcgga atgctgccga actgccagct ggtggccccc ggatgggtga
    37141 ggtgcgcagc ggacgcattg agtttgcggc tcaccttcgg tgcgagccgt tcggcgaggg
    37201 cagcgaggtc tcgtccgatc tccggttcgc gaccggcacc ggtgatccgg aaccgcacca
    37261 gcaggtgctc gggaacagct tccggacgcc acgcgtccgg cggtacctga acaccggtga
    37321 gggccaggat gccgcgagcc agcgcgatcc agatcggctc gtccgctcct ccggggtggt
    37381 ctgccagcca gtccagcgct cgggcagccg tgtccggtgc gggcaccagt tgggtgcgca
    37441 gtgctttcgg caggccgcgg acgagttcgg cggccacctg cgtccgcagc ccgggcacct
    37501 gccaggtgaa ggccgccggg ttgagcttcg gcagtaccgc gagcgggatg gtgacggtga
    37561 cgccgtcatg cccgctcccg gggtcgaagg cgtagtcgac ggctagttcc tcggccgcca
    37621 ccggccagtg gtcggggaag gaggtggtgt cgagttcggc gtccggggcc agcaggtcgg
    37681 cgacggtgag gtcgagaagg tgctcatcct tgcgccgcgc gtcgcgccac cagcggtcga
    37741 agtgcgccac agtgacaatc tcggcaggga tcctggcggc gtagaacgca gcgatcgaag
    37801 cgtcgtcgac tagcaggtcg cggcgccggt tgcgatcggc caggtcttcg gcttcggcgc
    37861 gcactgcctg attgcgcgcc cagaagtggt gccgggtgcg ccactggccc tcgaccaaag
    37921 cggactgaat gaagacctcg cgcgccacca ccgggtcgat ccgggcgtag ctgaccaggc
    37981 gctgggcgat gatcgggacg ccgagcagcg ttacccgctc gtaggccacg cactgtccgc
    38041 ccttggccga ccagtggggc tcggagtagt tccgcttgag cagatgcccg cccacggcct
    38101 ccacccagtc cgcgtcgatc ggggcgacgg tccgcgccca cagcctggaa gtctccacca
    38161 gctcaacggc catcaccaat ggcggattgg tacgcgcagc gctggagcca gggttgatcg
    38221 cgaaccgggc gccgcgcgcg ccgaggtact ccgccggacc gcgacgcctc ccacgttccg
    38281 gcttggccgt gctggccgca tcgatcaggc cgacgtgcga gagcaggccg gaaagcaccg
    38341 cagtgtggac ggacgccaga tcggcggtcg catcgttgcg gctgagcccg agttcgcggc
    38401 atgcctcgcg tagctgggtg tgcaggtcct gccattcccg cacccggaga aagttgaggt
    38461 actcgctgcg acacatccgg cggaaggcgt tgccggaaag ctcccggcgc tgctggcgaa
    38521 ggtactgcca gagccgcagc agggccgcga tgtcggaacc gtccggggtt ggctcctgag
    38581 cctgctcagc ggcgccgcca ccgtccatcg gaacccagaa ccggcgatgg gccagatcgg
    38641 cggcggcggt ctcctcgctc ggacgctcgc ggacgtccgg gatcgcgatg ccggcgacga
    38701 tcggcagcac ttcccgaagg caaccgagcc gggacgcctc caccagcatc cggccaagcc
    38761 gcggatcgac aggcagtcgg gccaactgac ggccagtcgg ggtgaggttg accgttccct
    38821 tgcccattcc ggagatggcg ccgagctcgg tcagcaggcg cagtccgtcg cgcacctggg
    38881 tcgcgtccgg cggttcaacg aacgggaagt tggtgatctc ccccagcccg gcttcagcca
    38941 tctgcaggat gacggacgcg aggttggtgc gcaggatctc cggctcggtg aactctggcc
    39001 gggccgcgta gtcctcttcg ctgtacaacc ggatgcagac gcccggacca agccgaccgc
    39061 agcggccggc ccgctggttg gccgaagcct gggagatcgg ctcgatcggt agccgctgca
    39121 ctttggtgcg cgcggaatag cgcgagatcc gcgcgaagcc ggggtcgacc acgaaccgga
    39181 tgccaggcac cgtcagcgag gtctcggcga cgttggtcgc cagcacgatc cggcggccgg
    39241 tgtgcggcgc gaacacccgc tgctgttcgg ccaccgacag ccgggcgtac agcggcacca
    39301 cttcggtgaa ccgcaggttc aggccgttga tggcgtcggc ggcgtcccgg atctcacgct
    39361 cgccggacag gaagaccagg atgtcgccgt ccagctgggt ggacagttcg gtgaccgcgt
    39421 cgcagatcgc gtccaactgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    39481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt
    39541 cgtgggagat ctcggccagc aggatgccgt cggtcatcac cttcacgcga gtctggcggc
    39601 tgcgctggct ggagaaccgc acctggaagc cgacggtctg cccgaccgcc tcgcccatct
    39661 cctgggcgat ccgggtggcc aggctgcgcg cggcgatccg ccgcggctgg gtgtggccga
    39721 tcttccgtct tccggccagc aggcagatct tgggcagctg cgtggtcttg ccggagccgg
    39781 tttcgccggc gaccacgatc acctggtggt tgcgcagcgc atcccggatc tcctcgacgt
    39841 gcgcgctgat cggcagcgct tcgtcgaagg tgagtacggg gtcagcggtc atagccgacc
    39901 cagtttagtg cgcggcggcg gttcaggagc cgtggctgtc cttgggccgg atcactgcga
    39961 tcgggcaact gccgtggctc atcaggccgc ggatgagtcc gcccaccgag taggtgggga
    40021 aggctgcgtg cacttccagc gcgaggaggt cgagttcggc cgttcgcgca gcgagaatgt
    40081 ccagcggcac gccgtactcg acctcgagtt cgaccgcgac gtccgggaag gcctcggcca
    40141 agggctgaat catctcggtc atccgctgcc gggtgacctt gaccgccgcg gcctgttgct
    40201 ctgcggtcgg gtgcccgccg gagaaccagc gtccctgcgg cgcccgacag acgctgatca
    40261 ccgacacctt gccgccacgg gactggcatt cgcgcagcgc ccattccaac gccaccgcac
    40321 cacgcgcgga ggtgtccacg gccacgccga tcagaccggc cggcgtctcg cggaccacgt
    40381 ccgtcgagga caccacgacc accgggcaat gcgccgcacc gaccactccg acggctgtcg
    40441 acccgacgaa ggccttttcc ccggtcgagg tcgaccggcg gcccacgacc accagcgatg
    40501 cgtccgaact catgcgggtc agtgccgccg cggtgttgcc gagcaccacc tgggtacgca
    40561 ctcgtccgcg ctccagaccg tgggccaccg cgacatcgat cgtggacgcg agcatctcgg
    40621 cagccccgag cttgatctcc tccgggtcga agaccacacc ccaggcactg ttcaacacag
    40681 tgtcatcgac agcatggacg accaccagtt cggcttcccg gtgtctggcc tcgacgatcc
    40741 cgtactcaag ggcgcgggcg gcgtcctcgg aaccgtcgaa cccaaccacc acacgggccg
    40801 gaccctcata gggcttcaac atctcaattc ctctccacgg cggccgacac cgcgcggccg
    40861 gagtaggtgg acggggtgat cacgatggtc aggccgctgt ggtccggcgc ccacggtgcc
    40921 ggcagcactg gttgggcgga gccatcgtgg tctgctgcga cgccgtgcac cagcacgctc
    40981 cagccggtcg cggtgtcgac atcgatgtcg tccacctcga aggcaacctc gacatcgtcg
    41041 gccagttcgc ccatcaccga tgcggggttc acccgaaaga agatgcggtc gtcgtggcag
    41101 ccgtaggtga ccggaaggac ctgcaaaccg gaggcggcca gccaagcgac ccggccgaca
    41161 cagtgactgc gaaccagctg acgacattcc tcacccgagg ggcgggtgaa atgtccagcg
    41221 gtatccatcg cgcgaatgct agcccgggcg tggccaccaa gactggtgaa ccgccgaagt
    41281 cagtggttct tcttaggtta tggccggacg gagccctcag cgcagccagg tggaggccgc
    41341 cacgatcacc gggatggacg cgatggtgga gatgaaaatg gcatcccggg cgagcaaggt
    41401 ccgcacgtgg taacggccgg cgatcaggta gatgttctgg gcggtcggca gcgcagcgat
    41461 caccgttacc gccagcaggt cggcggtgct cagaccgaac acccaggcgc cgagggcgaa
    41521 cgcgatcgcc ggatgcagca gcaccttgat cggggcggcg agcgccagtt cgcgggcgtg
    41581 acggccgcgg ccgggaagcg ggtcgagccg gagcgagatg ccgaacgcca gcagcatggc
    41641 cggcactgcg acctggccga tcatctcgat cggttcccag agcagcgtgg gtacttcgat
    41701 cccgagcagg ttgacggcca ggccggcgag ggtaccaagc gtgatcgggt tacgcaccgg
    41761 cagggacgcg taacgccacc aggtcaggcg caccccgtcc ttgcgcgcgg cgccgaggtc
    41821 gagcagcgcc agtgcggtcg gctggagcac cccgacctgg atcagaatga tcggcgccat
    41881 ccaggtcatg tcaccgagca cgtacgccgc gatcggcagg cccaggttgc cggcgttggt
    41941 gtaggccgcg cagagcgtgc ccaccacgcg tccaggcaga tcgccgccac cgaaccagac
    42001 gcgcgccgcg atcagatagg cgcccgcggc caacgcgatc gcgagggctg agaccagcag
    42061 cgtccggctg aacaggtggc tgaggtctgc gcgggagacc agcgaaaaca gcagcgcggg
    42121 cgaggccacc gtgaacgcca gccgggaaag catccggcgg tggtcctcgg tgagtacacg
    42181 aaagtgcgcc accacccagc cgaccccgat caccgaccag atggtgaggt atccgctcag
    42241 ggccgcctgc acgtgttcag cctaagccag tcaccaggac agaaaggtcg ccgcccaccg
    42301 cacaccccta cgatgtgccg gtggctgaac gcaaccatct gatcgacctg gcgcggatca
    42361 cgtccgtgct ggtggtggtc gtgttccaca ccctgttgtg ggtggtggcg gtgccggacg
    42421 ggcagttgct gatctatccg tgggcgccgg gtccgggatg gtgggcagcg agctggatat
    42481 tcaacatcgt gccggtgttc tttgtcgccg ccggctacgc caatgccgtc gtggtggaca
    42541 agtggcgcac tagcggggcg ccctattcgg cgtttctggt gctgcggggc ggccggctgc
    42601 tcggcccgat gacgctgttc gttgcggtct tcacggtggt cgggacggtc gccgcgtggg
    42661 cgggctggcc gatggagtcg gccacgctca gccgccagtt cgcccagttg ttgtggttcg
    42721 cggtggtcta ccagctgctg ctggccgctt caccactggc ggtctggctg cacgaccggt
    42781 tcggcgggtg ggtgatgctg ccgctgttgc ttggcgtgat cgcggtggac gcggccgtcc
    42841 gggccagcgg ggatctcggc ctgcagtggc tgaatctcgc cttcgtgtgg ccgctggccc
    42901 atcagtgggg catcgcctac caccggggct ggttccgtgg ttggcggacg cggactctgc
    42961 tcgcgctgct ggccggctgc gccgcggtga tcgcggccct ggtgttcgcc ctggactatc
    43021 cgccggcttc ggtggcctgg gcggacgtgc tggtggccaa cctgctgccg cccacggtag
    43081 ccatcctcgt gctcggactg gctcagacgg tggtgctcgc gctgcttgag caagccggag
    43141 tgggcgtatg tctgcgcgcg ggcacccaac aactggtgcg ggtggcgaac gcgctgctgc
    43201 tgacggtcta tctgtggcag atcccggcga tcctggttgg cgcgggggcg ctcgcgggcc
    43261 tgagcctgct gtggcccgcg gcagcgccag tgttgatgaa ccgggtgctg ctggtggtgc
    43321 tcgtgctcgg catcgtggtg actgccgtgc cctggctggc ccgggtggaa ctgcggttga
    43381 taccggcatc cggtaacggc atcgcgaagc cggggcacgc cgagatcggc tacgcactgt
    43441 cctgcaccgg caccttcgcg gtctggcagg cgggggcggt gctgcacccg tccgcaccgt
    43501 gggcagcggc cgcggccggg ctgttcgcgg ccggagtgat ggcgctgcgc cgcgtcagtg
    43561 ccagcacggt tgttggctag actggccgct ctattcaggg ggccagatgg atccgacgca
    43621 gtggcacgtg ccctacccct tggtggtggc cgcgctcttc gtgatcgtca tgctgcgcgc
    43681 caacggcacc tattggctgg gccgcgcggc ctcggccggt gcgcggcaca cccgcctagc
    43741 caagctgatg gactcccccg gctacctgcg tgcgacgcag cgcatcgacg cctatggccc
    43801 gccggtgatc tcgctgtcgt tcctgaccat cggcttccag acgctcgcga acctggctgc
    43861 cggggcgacc cggatgagcc tgcggcacta cctgcccgcg gtggtgatcg gctcggtgat
    43921 gtgggcattc ctgtacgcca ccctgggcac ggtcggagtg gacctgctcg gcgatctcta
    43981 ccgccagtcc cccgcgctcg ctatcggact ggccgtgctc gtgatggcct tgatcgccgc
    44041 ctacgtgatc tggcaggtgc ggcggcggac gctggccgga cgggtgccgc tcgccgaaga
    44101 cactgccctc gctgaggact gacccagcgt ccgcacaggc tggcggctta gcattggcac
    44161 atggctgaga ttcgcaagta cgacccggct aagggcaacg ccatcgtcga agcgctacgc
    44221 agcgccatgg gcgacccgat ccgtccggtc acgcccggag aaccggcgcc gacccgaaag
    44281 cccagcgttg ccccagccac caccacttcc tgggtgacct tcaatgtggc tcaggcttcg
    44341 gcttccgacg aagcggccga ccaggactga gcggtcagca ggccaagcgc gaccaccgga
    44401 acagccaacg ggctcggcca cccatcaagg atgaccgagc ccgtcgccgt atctcacatc
    44461 agctgcagcc gctggtggaa ccgcagccct cgcagatgta gcaggagcct gagcgccgca
    44521 tcttggtgcc gcaggtgagg cacagcggcg cgtccacgct catgccggag agctgttcca
    44581 gcacctcggc ggtcgagtgg gccttgaact caaccagggc gggctggttc ggtccgtcct
    44641 cgtgcacccg gacgtcgtcc gcggcatcgt tcttgagcga ctccgactcg atcagctcgg
    44701 cctcgtcctc ctcggacagg taccggccgg tctcgacgta gcgcgcccgc tcggccgcgg
    44761 tgtagatgcc gagctcggcc cgctcgtcga aggacaggta gtccagggcc agcctgcgga
    44821 agatgtaatc catgatcgac tgcgccatcc gcacgtccgg gtcgtcggtc atgccggccg
    44881 gctcgaactt caggttggtg aacttctgcg cgaactgctc cagcggcacg ccgtactgca
    44941 ggccgatcga gaccgcgatc gagaacgcgt ccatcacacc ggccaaggtc gaaccctgct
    45001 tgccgagctt aaggaacact tcgcccagct tgccgtcgtt gtactggccc gaggtcatgt
    45061 agccctcggc accggccacg gagaaggacg tcgtccggct tacccgcgac ttcggcaacc
    45121 ggttgcggcg cggccggtac tcaacccgca cctccggctc agccttcgcc gccggctcat
    45181 cggtcttctt gccatcgctc agcggctggc cgactttgca gttgtcgcgg tagaccgcga
    45241 gcgccttcag gccgagcttc cagccctggg cgtagacgtc ggcgatctcc tcgaccgtcg
    45301 ccgtctccgg caggttcacg gtcttgctga tcgcgccgga aaggaacggc tgcaccgcag
    45361 ccatcatccg cacatgaccc atcggcttga tcgcccgctg gcccatcgcg gtgtcgaaga
    45421 tctcgtagtg ctccggcttc aggcccggcg cgttcaccac gtggccgtgg ttggcgatgt
    45481 agtcgacgat cgcctccacg gtctcgccgg cgtagccgag ccgctgcagc gctcgcggga
    45541 tcgtctggtt gacgatctgc atcgacccgc cgccgaccag cttcttgaac ttgaccagcg
    45601 agaagtctgg ctcgatgccg gtggtgtcgc agtccatcat gaagccgatg gttccggtcg
    45661 gggccagcac ggacgcctga gcgttccggt agccgttctt ggcaccgagc gccaccacct
    45721 gggcccactc ctcggacgcg gccatgtgca gcggcttgtc cagctcgtcc cagatccgga
    45781 cgtcgtcatt ggcggcctga tgcttgcgca tcacccgctg gtgcgcggcg gcgttgcgcg
    45841 cgtagccgtt gtacgggccc acgaccgcgg ccaattcggc cgagcgccgg taggacacgc
    45901 cggtcatcag cgaggtgatg gacgcggcca gggcgcggcc cgcgtccgag tcgtagccgc
    45961 ggccgatcgc catcagcagc gcgccgaggt tggcgtagcc gatgcccagc tgacggaagc
    46021 tgcgcgtggt ctcgccaatg gcctcggtcg ggaagtcggc gaagcagatc gagatgtcca
    46081 tcgcggtgat gatcaactcg atggccttga cgaacgactc gacgtcgaag gtgtcgtcgt
    46141 cgcgcaggaa cttcatcagg ttcaggctgg ccaggttgca ggagctgttg tcgaggctca
    46201 tgtactccga gcacgggttg gacgcgttga tccggcccga ctccggattg gtatgccagg
    46261 cgttgatcgt gtcgtcgtac tggatgcccg gatcggcgca ctcccaggcc gccttcgcca
    46321 tcttgccgaa cagctcgcgg gcgtcgacct tctcgatcac ctcgccggtc atccgggcgc
    46381 gcaggccgaa atcggtgccg tcctcgaccg cctgcatgaa ctcgtcgctg acccgcaccg
    46441 agttgtt
//
LOCUS       DAOPRQ010000007        44501 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_629_length_44501_cov_13.022049, whole genome shotgun sequence.
ACCESSION   DAOPRQ010000007 DAOPRQ010000000
VERSION     DAOPRQ010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 44501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 44501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44501
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_629_length_44501_cov_13.022049"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..672)
                     /locus_tag="PKJ61_01485"
     CDS             complement(<1..672)
                     /locus_tag="PKJ61_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HOC12538.1"
                     /translation="MTQWFTESFKAEYEKQAAELGRVNIAVFGKTGVGKSTLVNAIFG
                     EPLAATGIGAPVTMGSHLYLDTRGSLGIIDTRGLELGKDDDQIIKELTKAIKDSRSKA
                     ISEQIHIAWYCIRGMDRRFEDFEERFIRTLDELGLPVLVVLTQVPMRDGQFHPDAVEL
                     ARQIEAKQLPIVGGRPFMTYAMRDQFTGQPPYGLMEVLGATFHVAPDAVRGALVAAQT
                     IDTRLK"
     gene            995..1567
                     /locus_tag="PKJ61_01490"
     CDS             995..1567
                     /locus_tag="PKJ61_01490"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HOC12539.1"
                     /translation="MAAPATEYRLRACLASSQPYPFAEADGWAAVRVPYVGGELAAEL
                     ILPPAGTAPSAISTDTLAKLHQALAASTSEPVELWLPTLDLAPKPLDLRGVLGDRAPS
                     VLCGAPNTDLSGIGPQDLCVSQAVQQAVLQLDEEGTKAAAVTEIGAVASGAPAQPEHK
                     LHLDRPFLLQISASSTGWPLFLAAVTDPRH"
     gene            complement(1586..2581)
                     /locus_tag="PKJ61_01495"
     CDS             complement(1586..2581)
                     /locus_tag="PKJ61_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC12540.1"
                     /translation="MILVNVLIGLGLLVLLALTFAPLESLGWWAREGADEAAATVQEL
                     AAIDSGPDDSAYDGYVVYLSGIGAVGGDSVPPEELPLIQNLSSRLTRLKVIHDVFPYS
                     VSNNGLTAQRPTAAVWRWVEKLRFKNPETLAGMLINARNAMQLFVCADRRYGPAYNVG
                     TAQEVMRALRRHGYPMGSGLPVTLIGWSGGAQISIGAAWYLGMGGRIPLQVISVGGMM
                     ADDYGLDRIQKLWHLRGSKDPLEKLGGILFAGRWPHAVFSPWTNALKDGRIEIIDLGP
                     MQHNVKDHYFDANSIAPDGRSYLQVTEDAIVAVLTGEPVKTVPLHSVPTRREVSA"
     gene            complement(2578..3249)
                     /locus_tag="PKJ61_01500"
     CDS             complement(2578..3249)
                     /locus_tag="PKJ61_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC12541.1"
                     /translation="MGILEFLGAVGRVIVGVLGLDVGNADWIAQHPQRLGIAVAVAAI
                     AGISTLLGDAVVIFFNRLRGWRFALTLALNGIGMVLLYLVQAAVIYVIGPLISGFDPG
                     FTAVVTAVLLATAPLVFGVFVLVPYAGPAIAGFLHVWGMVALWVIVDVVFRTDHWTAL
                     WITLISWLCMQVLSRAFAVPIRWIGDWIWRLVSGQPSMITGSDLLSGHLFLPLGQRFD
                     PEARS"
     gene            3472..4995
                     /locus_tag="PKJ61_01505"
     CDS             3472..4995
                     /locus_tag="PKJ61_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HOC12542.1"
                     /translation="MSLLRTKSIEQSIADTDEPEYQLKRSLSALDLTVFGVGVVIGAG
                     IFTLTGRAAHNVAGPSIVISFVVAAICCALAAMCYAEFASTVPVSGSAYTFSYASLGE
                     IFAWIIGWDLILEMFLGASVVAQGWSAYLGVFLEKIGVVLPPVISYGGLVDLPAILLV
                     VVLGGLVTIGIKESLRVNLALVALKLFIVLFVIVAGIGFINPANYSPFVPPTAPTEAA
                     TGWTQPLLQFLTGGAPTAFGIGGIFAGAALVFFAYIGFDVVATTAEETKNPQRDLPIG
                     IIASLAICTVLYCAVALVVTGMVPFDQLDPKAALANAFAFHGQAWMATLISAGAVAGL
                     TTVVLTLMIGATRLIFAMCRDALLPMNLAKVHPKYRTPWVITIIVTVAVALVAGLTPV
                     GVLEEMVNIGTLSAFVLVSIGVIVLRKKRPDLKRSFKVPFSPWLPIASALICGYLMLN
                     LSVETWLRFLIWLVLGFVVYFAYSRSRSRLATGVGIRPDMLDAMGHGHAPQDPDDQR"
     assembly_gap    5184..5283
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5356..6309)
                     /locus_tag="PKJ61_01510"
     CDS             complement(5356..6309)
                     /locus_tag="PKJ61_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC12543.1"
                     /translation="MLSALADASGLGCDSGMDVLRTSQLRAAGTNQRQLARSLELGER
                     TLVRHGAWATEAPKDDLAKHRQLVAGTWPLLASNAVLSHGSAAVLHGLPVWKPMLHRV
                     SITRASGGHGARTNQLHVRCARLDSADLIELDGYRVTSLERTAVDLARSVSYERAVAI
                     LDAALHAKADPQLLADMSAAARYTSGAGVLRRALEFADRRAESVAESISRVRMAEVGL
                     PVPVLQFEVFDDYGRWLGRTDFAWLEYGVVGEFDGAVKYTGTEREVAAAVMAEKRRQA
                     AIEAAGWVVVRWTWPDLNDRRAFRNRIATALADGQQRRHRP"
     gene            6625..8487
                     /gene="typA"
                     /locus_tag="PKJ61_01515"
     CDS             6625..8487
                     /gene="typA"
                     /locus_tag="PKJ61_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002518070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HOC12544.1"
                     /translation="MPVRADLRNVAIIAHVDHGKTTLVDAMLWQSGAFRENQDVNTRV
                     MDSMDLEREKGITILAKNTAVDHVLQDGSTVTINIIDTPGHADFGGEVERGLEMVDGV
                     LLLVDASEGPLPQTRFVLRKALAKNLPIIVVINKVDRADARIAEVVDEVYALFLDLLD
                     DDEAHHLDFPIIYCAAKAGRASLTQPADASLPDSANLEPLFQLIMDTIPAPLYTEGAP
                     LQAHVTNLDASPYLGRLALCRIVEGTLTRGQTVAWCRHDGSITNVRLSELLITKALER
                     VPAESAGPGDIVAIAGIPEITIGETLADPNDPKPLPLIHVDEPSISMTIGINTSPLAG
                     KVGKLLTARLVKNRLDTELVGNVSIRVLNTDRPDTWEVQGRGELQLAVLVEMMRREGF
                     ELTVGKPQVLVREIDGKLHEPVERLTVDVPEEHVGTISQLMGTRRARMEHMVNHGTGW
                     VRVEYVIPARGLIGFRTEFLTETRGTGIMHHVFEGYERWSGEIKTRQSGSLVADRTGV
                     VTGYALFNLQERGTLFVAPGDEVYEGMIVGENPRPDDMDVNPTKEKKLTNVRSSTGDE
                     LERLIPPRLMSLEQSLEFCREEECLEVTPKNVRIRKVTLDANERAKQRNRAKKA"
     gene            8870..10075
                     /locus_tag="PKJ61_01520"
     CDS             8870..10075
                     /locus_tag="PKJ61_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF480 domain-containing protein"
                     /protein_id="HOC12545.1"
                     /translation="MSLPVLDATEQRVLGSLLEKQRTVPASYPLSLNALRLACNQTSS
                     RDPVVDYSERTIQDAARALKDRGLLRLVWAGAGSRVVKYHQLLDEVLPLDEADRALLT
                     VLLLRGPQSPGELKTRTERLHGFADKAEVSERLEAMAAAEPPLVRELSLVRGQQDRRW
                     VHLLGPVPVGGADAEAPPAVDREAALAEGAAARDAKVIAAYDAAAAAYAAEFGEELIE
                     KPFDIWLLERVAEAADGPIMDLGCGPGHIAAFLADTGAEVHGLDASSAMIEQARSAYP
                     DLDFQVGRFHQLLRPRTAAAWGAIIAWYAFGHLAPSELGAVLRTASGTLNPGGLLALA
                     LELGDRVRHADSLCGVEVDLDYVLHDRQQVLAALAASDLVVDEWYVRAPVGGEADTKR
                     LYVLARKAG"
     gene            complement(10190..10957)
                     /locus_tag="PKJ61_01525"
     CDS             complement(10190..10957)
                     /locus_tag="PKJ61_01525"
                     /inference="COORDINATES: protein motif:HMM:NF024107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOC12546.1"
                     /translation="MKRAFRWLGLLLGLALVFGAGWAWPVLFRPPLIVGQDAITDQAT
                     IGVTPVIEDAGGRLIRVAPPAGTAAKDVLVIIYPGGLVRPQAYEWLARMLAVRGYETL
                     IPEFSFDLAVTDTDRASALIQRYADGRKVVLAGHSLGGAMAAQYLADELKAGREPVAG
                     LVLMAAYPPDGASLASAKLKVASLKGEFDNVADAAKIADGLRRLPKDSKLLQIDGGVH
                     SFFGRYGPQSGDGVPAVSRTDAEREIVNAIVSFLGRL"
     gene            complement(11296..12222)
                     /locus_tag="PKJ61_01530"
     CDS             complement(11296..12222)
                     /locus_tag="PKJ61_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HOC12547.1"
                     /translation="MSVRLTEGLDAGAVWHYGDPLREQRALLAGTAGVDLSHRPLFAI
                     RGADRLKWLNDITSQDFASLPSGTPTVAYILDPQGHLVHVFGAVDDGETLWAHTEPGR
                     RDSLVAWLRRMVFAARVEIIELGDEYSLVLPPRGGPAIVASAQVDATLGEVRAGTWAM
                     DALRIASGTPRIFLDTDEKTIPNELANPDDDRLGPAVHLRKGCYPGQETVARVYNLGR
                     PPRRLTLLHLDGSAERLPELGADVFLDGRVVGRMGTAERHFELGPIGLALLKRTLPTD
                     AELVVDGIAAAQETIVDPDAGLHWRPQPGLGR"
     gene            complement(12219..12716)
                     /locus_tag="PKJ61_01535"
     CDS             complement(12219..12716)
                     /locus_tag="PKJ61_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013161821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FABP family protein"
                     /protein_id="HOC12548.1"
                     /translation="MAFEIPADLNPALVGVAWLRGRWEGTGFRQWPGEEKVGYRIQVD
                     FADNGEDFLHYLAQAFEVDSDGSPTRPLFMETGFWRPLPDGTVDVMMCSPDGFAEIWF
                     GKLQPGRLDLITDAVARTRDAKVSYTAGRRLYGNVEGKLLFSFDRATEEHELQPYIWA
                     TLERR"
     gene            13411..13842
                     /gene="dtd"
                     /locus_tag="PKJ61_01540"
     CDS             13411..13842
                     /gene="dtd"
                     /locus_tag="PKJ61_01540"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOC12549.1"
                     /translation="MRAVVQRVSRARVEVDGEVIGRIEDPGLLVLIGVTHTDTESVAD
                     KLADKTWQLRILEGERSCAEASAPLLVVSQFTLYADTRKGRRPSWSAAAPGTVSEPLV
                     NAYIAALRRRGAHVETGRFGAMMAVTSTNAGPMTVLLEADA"
     gene            13965..14642
                     /locus_tag="PKJ61_01545"
     CDS             13965..14642
                     /locus_tag="PKJ61_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOC12550.1"
                     /translation="MAQLLLVSNEVARGTPAEISLPVLSLLAHDIRGVAADTLALTES
                     ARADLVVLDGRADLPGARTMARLFNSSGSTVPLLLVLTESGLAAVATDWGLADVVLDT
                     AGPAEFEARIRLLVTSAANDGLISAGGIVIDDAAYSAMLDGAPLDLTYTEFELLKYLV
                     QHPGRVFSREHLLADVWGYDYYGGTRTVDVHVRRLRAKLGPEHEALIGTVRNVGYRFS
                     PDGSARR"
     gene            14665..15549
                     /gene="mshD"
                     /locus_tag="PKJ61_01550"
     CDS             14665..15549
                     /gene="mshD"
                     /locus_tag="PKJ61_01550"
                     /EC_number="2.3.1.189"
                     /inference="COORDINATES: protein motif:HMM:TIGR03448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035447 - mycothiol synthase activity
                     [Evidence IEA];
                     GO_process: GO:0010125 - mycothiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycothiol synthase"
                     /protein_id="HOC12551.1"
                     /translation="MPTNRLSAAQAEQVLDLAAAATAHDGVDPLNEEARFALRDDSAE
                     HYLHHVGDRLAGYLIWLPLHHTGQLVVHPEFRRQGIGRTLVEQAFGVIDGAPAGSTTR
                     PVAIWAFGHLRAAQGFASALELSPVRGLLIMDRPLAEPVTPHVPDALQLRGFELADAA
                     AVLTVNAAAFAHHPEQGSLDEAGLHARMAEPWFDPSGLILGFDADGLAGFHWTKRMDA
                     RTGEVYVMAVAPRAQGRGYGKALLNAGLAHLQRSGCDRVLLYVDMADQVAVRMYESAG
                     FRVTHRDVLLAPKPKEQQ"
     gene            15546..17666
                     /locus_tag="PKJ61_01555"
     CDS             15546..17666
                     /locus_tag="PKJ61_01555"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002528967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HOC12552.1"
                     /translation="MTVEEQTVAADAAPTPPDLPADRFSDRELSWLAFNNRVLDLAKD
                     AKRVPLLERAKFLAIFASNLDEFFMVRVAGLKRRMAAGVAVTSNSGLMPRELHDAILT
                     RTRELVVDHAQVFAEDIRPALAQHGIEILRWDELTSDEREKMTALFENRIFPVLTPLA
                     VDPSHPFPYISGLSVNLAVLLRNPATGVRQFARVKVPTILSRFVPLGGGRFVPLEEVI
                     ARHLGQLFSGMEILGHSTFRVTRNEDVEVEEDDAENLLFALEKELLRRKIGRPPVRLE
                     VEEDIDDRMLQLLISELDIAEKEVFRMPSPLDLRGLFAIADLDRESLLYPSFLPKTHP
                     ELAEVETSRPADLFAALKSREVLLHHPYDSFATSVQRFIEQAAADPAVLAIKQTLYRT
                     SGDSPIIDALVEAAQAGKQVLALVEIKARFDEQANIAWARKLEKAGCHVVYGMLGLKT
                     HCKLSLVVREEETGLRRYVHIGTGNYNPKTARMYEDMGLLTSNNIITDDVARLFNHLS
                     GMAQETHYKRLLVAPHGVRRGLIENINHEIENHRAGLPARVRIKVNSIVDEAVTDAMY
                     RASQAGVPVDLWVRGICSVRPGVPGLSENIRVRSILGRFLEHSRLYWFANGGNPTVGI
                     GSSDLMHRNLDRRVEVIVGLNNPKHIDQIEGLFELAFDEGTASWWLEGDTWTQRTLDA
                     DGEPLLDIQDHLIATMGQRRAVER"
     gene            17670..18563
                     /locus_tag="PKJ61_01560"
     CDS             17670..18563
                     /locus_tag="PKJ61_01560"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOC12553.1"
                     /translation="MANTRVSKQITAAGTVVLRENPDGQTEVLLVHRPRYQDWSLPKG
                     KLDPDEYLVGCAARETREEAGVNVRLGIPLDPIQYPVSAGTKTVFYWRSRVVGNGRQK
                     ANGEVDDAAWFSVREALDLVTYADERPLIRQAVALPDSTPLLLIRHGKALPRSDWDGI
                     DSERPLDERGRLQSQRLTPLLDAYGVGRLVSSTSARCLQTLLPHSHASGLVLEAEPAL
                     SEERFATEPKAVTPLLKRIAKQAAETGKPAAICGHRPVLPTMLAALKLAPRSLQPGST
                     LVVHLAPDASVLAVELHKPEV"
     gene            complement(18592..19626)
                     /locus_tag="PKJ61_01565"
     CDS             complement(18592..19626)
                     /locus_tag="PKJ61_01565"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOC12554.1"
                     /translation="MGEVAGDGTAVYRPPTLTDVARLAGVSTMTVSRVINGHAKVATD
                     TRERVRAAMRSLDYRPNIMARGLASGRSRSIGVLTVDTTLFGPRAALRGIELAARDRG
                     YSVTVAHLDDPDRNAVAQGVHLLQSRSSDGVILLQPLISEVHEVEPPADLPPMVAIHA
                     GTPGNYPVVSVDQRLGARLATEHLLKLGHRTVWHLAGPNLWYEGTERVTGWQEALTSA
                     GAPIKPIQRGDWSAASGYQAGRELLRDPEVTAIFVANDAMALGVLYAAHELGLRVPDE
                     LSVVGFDDTPEAEFFWPGLTTIRQDFDEIGQLSVELLVQLIETGKARTQHLVLPPKFV
                     LRRSTAAPRR"
     gene            19825..20904
                     /gene="pstS"
                     /locus_tag="PKJ61_01570"
     CDS             19825..20904
                     /gene="pstS"
                     /locus_tag="PKJ61_01570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HOC12555.1"
                     /translation="MKIKPIAAVLATATAFTLVGCAANEAAGTGGTSSAAAQTLTGKG
                     ASSMKAAQEKWVADFQTAHTGITVNYSPEGSSAGRSAFTAGAVQFAGSDRPLKDEEMG
                     AGKFATCAADSNALNLPVYVSPIAIIFKLDGIDKLSLDPATAAKIFAGQITKWNDPAI
                     VALNPDAKLPDSNITAVHRADGSGTTNNFTDTLHQLAPEVWTESAADDWPAAFGGEAA
                     TGTSGVVSAVSGGQGTIGYADASAAKNVGIASLAVNGKSLEPSAEAAAAVIDHSQTVA
                     GRGEHDLAYSLDRTSEGVYPAVLVSYAIVCETYKDAATAKLVKDYIGYIASAEGQQAA
                     ATAAGSAPLSAAIQAKVKAAVDSIK"
     gene            21000..21983
                     /gene="pstC"
                     /locus_tag="PKJ61_01575"
     CDS             21000..21983
                     /gene="pstC"
                     /locus_tag="PKJ61_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016456661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HOC12556.1"
                     /translation="MTHPLSTDSGPGPAGAPEAALAADPKRFGDRVFRGFATGAGVLI
                     LVILALVAGFLLIQGAPALFADPDRLASQGYGEFASWVLPYAFGTVWAAVLALAMAVP
                     VAIGIALFISHFAPRRIAQPLGYLIDLLAAVPSVVFGLWGSTLLARALIPLYGWLNAN
                     LGFIPFFAGITSGTGRTILTAAIVLATMVLPIITSLCREVFLQTPRLHEEAALALGAT
                     RWEMIKMAVLPFARPGIIAAVMLGLGRALGETMAVSMVMSGGRNITFEVLTSQNPNTI
                     AATIAQNFAEAYGLKTNQLIAAGLVLFLITLAVNMLARWIVSRRAEYSGAN"
     gene            21983..23095
                     /gene="pstA"
                     /locus_tag="PKJ61_01580"
     CDS             21983..23095
                     /gene="pstA"
                     /locus_tag="PKJ61_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HOC12557.1"
                     /translation="MSTLNPSAGPLGARPTGGTGPLLGGDTLTSGRLPKATTWVALVA
                     SAIVGFAISVLATGAANAGLTVLFGYVLFLLIMEVASRVVEGPRQASDRRAGNLIIGA
                     FLLALLPLGSVTWEAVSNGMARFDPSFFTNSMRNVVGEGGGGVHAIYGTLYVTGMASL
                     ISVPIGLLTAIYLAEYGAGNRLAKLITFFVDVMTGVPSIVAGLFAYSLMALLVGPGAV
                     FGFSGSVALSVLMIPVVVRSCEELLRLVPNELREAAYALGVPKWVTIVRIVIPTAISG
                     LVSGVILAIARVIGETAPLMIAAGFTASLNNNLFANPMMTLPVFVYDAYAHPGVEPAP
                     YLERAWTGALTLILIVMLLNLIGRLIAARFAPKTGR"
     gene            23108..23887
                     /gene="pstB"
                     /locus_tag="PKJ61_01585"
     CDS             23108..23887
                     /gene="pstB"
                     /locus_tag="PKJ61_01585"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004810563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HOC12558.1"
                     /translation="MSKRIEVTDLNVYYGSFRAVEDVTMTIEPRTVTAFIGPSGCGKS
                     TFLRTLNRMHEVIPGARVEGEVLLDGENLYGTGVDPVRVRRQVGMVFQRPNPFPTMSI
                     RENVLAGIRLNFAKMPKAAQDELVERSLRNANLWEEVKNRLDLPGSGLSGGQQQRLCI
                     ARAIAMAPEVLLMDEPCSALDPISTLAIEDLISELKEKYTIVIVTHNMQQAARVSDVT
                     AFFNIAGTGSPGRLIESGPTDKIFSAPDIAATEDYISGRFG"
     gene            23942..24814
                     /locus_tag="PKJ61_01590"
     CDS             23942..24814
                     /locus_tag="PKJ61_01590"
                     /inference="COORDINATES: protein motif:HMM:NF022169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2510 domain-containing protein"
                     /protein_id="HOC12559.1"
                     /translation="MSNAGWYPDPGGQPGMYRYWTGTEWTSAITANPAGTQPPSYSSG
                     QPAGVPGQGRKRGATGWWLGGLAALAAVVVAIWFVAQSFGTTGGILGGDDPTTPGGGA
                     TQLICPPGDNSTASAPPAGEPGWTTGGRLSFPTLGDPWTTGHDYEVPYGSVATVQTVL
                     DQDNYDGRGNSWVASVLVSDLYVGDGFASPKQGAEIVLKCVLGKYYADTIVTQNPISS
                     AKHDVDGHSGWLIETDLSFSISNLNATSERVLLLVVQTGTEEYGLFYASVPNTLDHLM
                     PDARQALANLRVEA"
     gene            complement(24888..26057)
                     /locus_tag="PKJ61_01595"
     CDS             complement(24888..26057)
                     /locus_tag="PKJ61_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020387750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HOC12560.1"
                     /translation="MPPDRPSEPPSPTTPTSAPTTPTPAPTASASVQPEESEPPTPSD
                     EPELELCLAEAQAMSLDARIGQLIMVGVTDGLDAAERKAITSYQLGSVVLLGNQSGGV
                     ADTAALAAELTALVPDAGMLLSVDQEGGVVQRLTGTGFRKIPSATKQAELPLDSLQAR
                     ARNWGVELIEAGVQLNLAPVADVVPAKKVKSNAPIGKLNRGFGSAPELVAEKVRAVIN
                     GYSAAAVGTTVKHFPGLGEVVGNTDHASGVVDDVTTADSASLLPFRTAIETGVAAVMI
                     SSARYSKIDAKNQAVFSPKVIGLLRDWDYDGVVISDDLGAAVSVSKIPAGQRALRFLS
                     AGGDVVINASPALVGPMVDAVKVEAGKDEAFAEQVTRSVARVLRMKQGLGIVSCG"
     gene            complement(26201..27583)
                     /locus_tag="PKJ61_01600"
     CDS             complement(26201..27583)
                     /locus_tag="PKJ61_01600"
                     /EC_number="1.8.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012923469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mycothione reductase"
                     /protein_id="HOC12561.1"
                     /translation="MRHFDLVVIGSGTGNTIITKQFADWRVALIDGQKWFGGTCLNVG
                     CIPTKMFAHPADLAESARRASILGVNTGQVSVDWPAIRDRIFGRIDPIATSGEEYRRN
                     SPNVTLFREHARFVSAKVLQVGEELISADQIVLAAGSRPRLPDVPGADAAELGERVHT
                     SDSIMRLERLPKSLIIVGGGLVAAEFAHIFAAFGTQVTMLYRGDHLLRAFDHDVSKRF
                     TKLLSRRVVLRLGQHLASIETTPLGVGVGTTDDDGIEYFFEAEQLLLATGRVPNSDTL
                     DLEKAGVEVGADGYVVVDEHQRTSAAGIWALGDVSSPWQLKHVANHEARVVQHNLLHP
                     DDLCSSDHRFIPMAVFSGPQVATVGLTEDDCRQQGRDYVVGVRDYGSVAYGWAMEDTD
                     HFAKVIADRGTGQLLGAHIIGPDASNLIQPLIQAMATGLAAATMARGQYWIHPALTEV
                     VENAVLALDL"
     gene            complement(27852..28772)
                     /gene="ccsB"
                     /locus_tag="PKJ61_01605"
     CDS             complement(27852..28772)
                     /gene="ccsB"
                     /locus_tag="PKJ61_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015583280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HOC12562.1"
                     /translation="MVTSAVLYLLAMTVHAAEWASARAVKLPEPVLVGAAAEPDGVVT
                     PSGVLVEDAPPARSQRIELLGRAALGITFVAAATHILGVVLRGVAANRAPWGNMYEFI
                     TSALAAVVIVYLVMALRGTRWLGLPATLLLAIGQGLAVTVFYVDVSELMPALHSVWFR
                     IHIFAAVAAAAAFNIGGIAALIYLWRSRAEAKGSVRGYLARIPSADALDRISYRFHAF
                     AFPLWTFTIAAGAIWAQYAWGRFWGWDPKETWSLVTWIIYAGYLHARATVGWKGRRAA
                     IVALIGLASFWFNFVGINLLTSGLHSYAGI"
     gene            complement(28796..30331)
                     /locus_tag="PKJ61_01610"
     CDS             complement(28796..30331)
                     /locus_tag="PKJ61_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014598414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HOC12563.1"
                     /translation="MKSVFSWLRFAWTTLTSMRTALILLLILGIAAIPGSFLPQRPTD
                     PVGVADHLRANPGVGEWLNRLGFYDVFGSPWFAAIYLLLFISLIGCILPRTATFVRAL
                     RQPPSPAPGRLSRLPEFTTGLVVASGDEVLAAAEAHLRAHKYRVRRESSAISAERGYL
                     REAGNLVFHICLVTTLIGLAWGALFSFKGTAIVVEGQAFSNTLTQYDEFGGGAGFRAA
                     QLSPFTVRLDSFEAEFETGPVQQGAARLFRAEVTVTGAGGAPSQQVLEVNAPVTVGGN
                     SVHLLGHGYAPVVTVRDGNGDVAFSGPVVFLPQDANFRSDGVIKAPDARPERLAFEGV
                     FLPTAGEDGANGMVSVFPDVLNPQLLVNAWAGPPKAETGMPENVYSLNKTGLTQLTKP
                     NGDPVRAGLAVGTSYELPDGRGSISFDGWKRWTKLQISNTPGGWLTLGSVMLAVLGMS
                     ISLSVKPRRLFVRAVPEPTGQLTISVAGLDRVDGREGLAAEVRALALACGLSNSEADK
                     EEP"
     gene            complement(30331..31044)
                     /locus_tag="PKJ61_01615"
     CDS             complement(30331..31044)
                     /locus_tag="PKJ61_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002545769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HOC12564.1"
                     /translation="MLLALPVAVLAGLVSFFSPCVLPLLPGYLSYATGLGAVEITEGT
                     GPRRRVLLGTVGFISGFALVFVATGALAGTLGSALIAWQRPITIAVGLLSIALGLIFT
                     GWLPFGQNTVRLNAVPRLGVAASPLLGIVFGLGWTPCIGPALSVVLSLALNEATALRG
                     GVLALAYALGLGLPFLVAGLAFARMARTLDWVKRHHRGLQIAGGVAMIAVGLLLVTGW
                     WDAVMAVMRQWVSGFQVVI"
     gene            complement(31092..31649)
                     /locus_tag="PKJ61_01620"
     CDS             complement(31092..31649)
                     /locus_tag="PKJ61_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004810534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOC12565.1"
                     /translation="MRAYRLLVAMAAATLFVGCSSAPSSSGGFVGGDGSITVLAIADR
                     PEAPAIEGTTLDGATWRSADVSGKVIVYNVWGSWCSPCRAEAPALAAASKQLAGQAIF
                     VGLNTRDIDMAAPKAFVRAFEVPYANLFDPDGALLLKFSGQLPASAIPSTLVVDADGR
                     VAARIIGETTQATLVGLVEDVAAGK"
     gene            complement(31732..32379)
                     /locus_tag="PKJ61_01625"
     CDS             complement(31732..32379)
                     /locus_tag="PKJ61_01625"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020392280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOC12566.1"
                     /translation="MEISVVHLVRHGKVENPEGVLYGRQPGFGLSELGQRMAERLGEY
                     FADANLVHLVSSPLQRALETMAPIAAKHPELEVHLDERVIEAANVFEGKSFGKRNEIL
                     FRPSSWWAMRNPLRPSWGEAYVSIVARMQAALADAAANSVGGEAVVVAHELPIWMARS
                     WAEGRRLVHDPRRRQTSLASVTSFTYHDGHLVRVDYAEPCADLVPPKAKKFRPGA"
     gene            complement(32391..32672)
                     /locus_tag="PKJ61_01630"
     CDS             complement(32391..32672)
                     /locus_tag="PKJ61_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HOC12567.1"
                     /translation="MDQARVVLLVRDGCHLCDEAVAIVRAVCASADATWTTVDVDSDL
                     ELRAEFGDHVPVTFVDGARHAIWHVDAAALAAALGSRRGSPAAYPWGHS"
     gene            complement(32698..32799)
                     /locus_tag="PKJ61_01635"
     CDS             complement(32698..32799)
                     /locus_tag="PKJ61_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003402602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AURKAIP1/COX24 domain-containing protein"
                     /protein_id="HOC12568.1"
                     /translation="MGSVIKKRRKRMAKKKHRKLLKKTRIQRRRAGK"
     gene            complement(32934..33137)
                     /locus_tag="PKJ61_01640"
     CDS             complement(32934..33137)
                     /locus_tag="PKJ61_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020388010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOC12569.1"
                     /translation="MNDPVLPLGTVKFLKVAEVAAALRVSRMSVYRLIHSGDLEAIRF
                     GRNFRVPEHAVDAYLRGSFYRAG"
     gene            complement(33249..34130)
                     /locus_tag="PKJ61_01645"
     CDS             complement(33249..34130)
                     /locus_tag="PKJ61_01645"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOC12570.1"
                     /translation="MPGLSEAKGAAPGPVGWFVGLATLDVIQLVDVLPAPNQKTTAKQ
                     TWLAAGGPATVAAIAFAALGGRAVLWTALGGGAAADAIRADLASAGVEVIDAAPDGFE
                     VATSSVLLDAATGERAVISGSGHRPHLASPPKPDLTAADVVLIDGHHAELAAAAAREA
                     SASGLPVVVDAGSHKPVFDQLLTLSTDVICSADYVHPAGLTPAELAAGRRLLAVSHGA
                     DALVWWSDGEHGVVQPPSASVVDTLSAGDVLHGAYAFGLASGWSRLAALEFAVQVASI
                     RVRRLGPFAWRHAMAAG"
     gene            complement(34118..34870)
                     /locus_tag="PKJ61_01650"
     CDS             complement(34118..34870)
                     /locus_tag="PKJ61_01650"
                     /inference="COORDINATES: protein motif:HMM:NF037170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOC12571.1"
                     /translation="MGAARPFWLTAFLDFPAAEYDAGVRFWTHATGFDASAPRGENSE
                     FATLLPASGDDYLRVQRLGDGVTRLHLDLHVVDPWAAAEAAEDLGAELVDDSRHSYVV
                     LRSPGGIVLCFVTQPAATVPPAAVWPAGQTSRLAQVCWDVPRARFSAEAAFWQAILAG
                     EWTPPDAGEAITRKAGELALDLRLQPAEIAREVVGHLHQVTDDRSAEVARLTAAGSIT
                     RSVRETQTMLETPGGVPLCVLDQQRGGIACRA"
     gene            complement(34872..35672)
                     /gene="proC"
                     /locus_tag="PKJ61_01655"
     CDS             complement(34872..35672)
                     /gene="proC"
                     /locus_tag="PKJ61_01655"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008356054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HOC12572.1"
                     /translation="MTKLAVIGAGVMGETLIAGLLRAGWAADEITAADRHNARLAEME
                     AGYGIRTGSTAEASAEADTVVVVVKPQDAGEVLPEVGGAIRPGTLVVSLCAGLVTSRI
                     EAAMPEGTPVVRVMPNTPAQVDEGMAAISAGSAATAEHLEHVTGLLSAVGKVVTVPEK
                     YQDAVTAISGSGPAYLFFVVEAMIDAGVMLGLPRDIATTLVVQTMYGSAKLLSESGVH
                     PTVLRERVTSPGGTTAAALRELEDHRVKAAFMTAMEAARDRSRKLASS"
     gene            complement(35669..36700)
                     /locus_tag="PKJ61_01660"
     CDS             complement(35669..36700)
                     /locus_tag="PKJ61_01660"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002531683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOC12573.1"
                     /translation="MAPMRVGVFGATGQVGGVMRTLLAERDFPVDELRFFSSARSAGK
                     PLSWRDREILVEDTATADFSGLDLALFSAGATMSREFAPKVAAAGAIVVDNSSAWRKD
                     PDVPLVVSEVNPEDAIDPPKGIIANPNCTTMAAMPVLKPLHDAAGLRRLTVATYQAVS
                     GSGVKGVQALADQTAAAADPARLALDGRAVPFGDPAPYLKPIAFNVVALAGALVDDGS
                     GETDEEQKLRHESRKILHIPDLLVAGTCVRVPVFTGHSLSIHAEFEEEISVTRATGLL
                     ATAPGVVLTDIPNPREAAGTDPSYVGRIRADQSVADGHGLVLFVSNDNLRKGAALNAV
                     QIAELVAKR"
     gene            complement(36775..37134)
                     /locus_tag="PKJ61_01665"
     CDS             complement(36775..37134)
                     /locus_tag="PKJ61_01665"
                     /inference="COORDINATES: protein motif:HMM:NF022158.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HOC12574.1"
                     /translation="MTGFGIMFAVVAVFIGIVFVVVIGAIIFGLVKAGRTAAANQAAP
                     EVNAVAMIVDKRVETHGGGESSVSQTHFVTFQQPSGERFELQVSASEYGLLVVGDRGT
                     VTMKGTRYLGFNREIMR"
     gene            complement(37175..37966)
                     /locus_tag="PKJ61_01670"
     CDS             complement(37175..37966)
                     /locus_tag="PKJ61_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HOC12575.1"
                     /translation="MAAAELNPEPLTSDLPADGRTLPGIELLLVLGVSLGQSAVYSIL
                     SILEKATRGTPLNQQTTSINSSVVPDRPWLDLSYQVAGVVFPLVPALLALYLLQRSAD
                     RTRIGFDLRRPGFDLSRGFGLAAVIGIPGLALYFVARAFGLNTNVAPANLAANWWTVP
                     VLIALAAMNAILEEVVMIGFWFVRATQLKWAPWVVLLSSAVVRGSYHLYQGYGGFVGN
                     IVMGIVFGLAYWRFKRVGPLVVAHFLLDFFAFVGYSLIAPYVSWL"
     gene            complement(37966..38781)
                     /locus_tag="PKJ61_01675"
     CDS             complement(37966..38781)
                     /locus_tag="PKJ61_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOC12576.1"
                     /translation="MPSVNASAPKLALSTSSVYPESTAAGFALARQLGFDGIELMVGA
                     DPTSADVDAVQRLAEYHEVPVVSVHAPTLLLTQRVWGPDPWEKLERSAMAAHQLGADV
                     VVVHPPFRWQRSYASGFTEGIRRLETTTGLTFAVENMYPWRGPGGGEVRAYAPSWDAS
                     LLDCDHLTLDLSHAATARQSSCALVHDWGERLAHLHLTDGSYGATDEHLAPGAGDQDA
                     ATVLATLAAQGYRGHVVLEVQTRGMERADRVKLIADSLTFAREHLAVGAGRAG"
     gene            38961..39596
                     /locus_tag="PKJ61_01680"
     CDS             38961..39596
                     /locus_tag="PKJ61_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002544240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOC12577.1"
                     /translation="MHVDHLTFAVGSAGLKGESKRLGALLGERFRDGGFHPRFGTQNH
                     ILPLADDRYLEVVEVLEHPAAEKAPFGQAVRSRSEMGGGWLGWVVSVDDLAPFEDRLD
                     RQAVHGSRHFPDGRLLEWDQIGVKGLIADPQLPYFLKWDSDPSVLPSALAGTIRLEKI
                     EIAGDRSRVEDWLGIQIGDEFDGVRIKFTRPAGQPGIQSVTFDTPHGTVRI"
     gene            39815..41209
                     /gene="radA"
                     /locus_tag="PKJ61_01685"
     CDS             39815..41209
                     /gene="radA"
                     /locus_tag="PKJ61_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002517052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HOC12578.1"
                     /translation="MAKSTSNSYRCTECGWTTPRWVGRCGECQAWGSVTEGSAPSARV
                     ASSVPASPALPIGEVPLEAARAIPTRLKELDRVLGAGLTPGAVVLLAGEPGVGKSTLL
                     LEVASRWARAGRRTLYVTAEESAAQVRLRAERTSAVIDGLYLAAENDLGNILGHIEQL
                     SPELLVLDSVQTVQVPGADSVAGGVAQVKEVTAALVRIAKQRALPVLLVGHVTKDGNV
                     AGPRTLEHLVDVVLSFEGDRMGGVRMLRAIKNRYGPADEVGCFEMVESGIREVTDPTG
                     LFTSQHTEPAPGTCLSITMAGRRPLLTEVQALVAPNSGPVPRRVTHGIEGGRVAMILA
                     VLQRRAGVRLHTRDVYVSTVGGAKVSDPATDLATALAIASAACDEPLWQPVIALGEVG
                     LSGELRRLPGVDRRLAEAARLGFKLALVPSGSGARTQGLKVVEVGNVRDALAAMQLHP
                     RRKREEQRPHLELA"
     gene            complement(41328..>41960)
                     /locus_tag="PKJ61_01690"
     CDS             complement(41328..>41960)
                     /locus_tag="PKJ61_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC12579.1"
                     /translation="GMGSVMHPVGKQPVWVYWARRAALVAAALLVLWLVLSMFQPAPN
                     APVAAVPATPSAATTPTAVASPSASETPSAAPTPTGPLTCDGTNSDLSVAGYQKVKQD
                     GKQLFKVAITNKGSAECVLDLSPETFTFAITSGTDKIWTTQDCAKWGPTKKTTLKSKK
                     AYEFTVEWPLKRSASGCRNTKDLIKPGTYVGTATLADALSAKQVFVVRKA"
     assembly_gap    41961..42060
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            42261..42932
                     /locus_tag="PKJ61_01695"
     CDS             42261..42932
                     /locus_tag="PKJ61_01695"
                     /inference="COORDINATES: protein motif:HMM:NF025198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HOC12580.1"
                     /translation="MTNPYARPYERPEDADLTIPLVPDSGYSQPSQPPFTEETLVDPL
                     PQAPRDPWGVASAQEFGYNPTPGQAPTGSSPQPAQTQPNYTQPQVGQQSSYQPYPASD
                     YPPMAAYPTTAQPTQPSAVAPWSASAPVPYAFSEAALPEHPSATTALVLGILGIVGLW
                     PLGPIAWIVGAKAKREMRAEPGRWRSSGSLTTGVVLGVITTVLMVLMTLAILFFVFLV
                     AVASR"
     gene            43002..44435
                     /locus_tag="PKJ61_01700"
     CDS             43002..44435
                     /locus_tag="PKJ61_01700"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOC12581.1"
                     /translation="MSERSDLEFLRMLHRLARSAESAETSRPGNLTRLGALVAAHLGV
                     PAEANQLLSLPLPAHRLVDADLALSHLADADPDTTVVGISGFARHEDSLAALLVNEHA
                     DVTPGPVDYTWLSTGPDTRRQVVSNGVRLFRLGDRPVAVWQRASDPRSGEERATVQVA
                     AATAADSEQVIAQVQNLMAELSVLRGQVVSFTGNEFQPGMAGISFHHRPQVEAGAIVL
                     PEGVLDRVVRHAVGIGQQAETLRRHGQHLKRGLLLYGPPGTGKTLTVRHLIGEARQTT
                     CILLSGNSLGMVTAAAELARAMQPALVVLEDVDLVAADREFYGGPQPLLFAILDALDG
                     LDGDADVAFILTTNRADLLEPALAQRPGRVDLAVEIPLPDAAARRRLFELYAAGLPFD
                     PAAVHTVAEAAAGVTASFAKEAVRRAVLEAALADREPTDADLSAAAAEMLAGGAGLTR
                     ALLGSPDARHEHYGLIGFADHPVGLSD"
BASE COUNT         7285 a        15465 c        14820 g         6731 t
ORIGIN      
        1 cttaagccgg gtatcaatcg tctgcgctgc gacgagggcg ccgcggaccg cgtccggagc
       61 cacgtgaaag gtggccccga ggacctccat caagccgtag ggcggctgac cggtgaactg
      121 gtccctcatc gcgtaggtca tgaacggacg cccgcccacg atcggcagct gcttggcctc
      181 gatctgccgg gcaagctcga ccgcgtccgg gtggaactgg ccgtcccgca tcggcacctg
      241 ggtgagcacc accagcaccg gcaaccccag ttcgtcgagg gtgcggatga agcgttcctc
      301 gaagtcctcg aagcgacgat ccatgccgcg gatgcagtac caggcgatgt ggatctgctc
      361 gctgatcgcc ttgctgcggc tgtccttgat cgccttggtc agttccttga tgatctggtc
      421 gtcgtccttg ccgagctcaa ggccgcgggt gtcgatgatg ccgaggctgc cgcgggtgtc
      481 cagatagagg tgggagccca tcgtgaccgg ggcgccgatg ccggtggctg ccagcggttc
      541 gccgaagatg gcgttgacca gggtcgactt gccgacgccg gtcttaccga acacggcgat
      601 gttgacccgg ccaagctcgg ctgcctgctt ctcgtactcg gccttgaatg attcggtgaa
      661 ccactgcgtc acgcgcccag cctattgggg aggtcgacaa ccgccatggg ttgtcgcccc
      721 gagcctcccc ggatcgaggt gtccgactcg gcccggatcg ggatcggcat ggtccggctc
      781 ggcccggatc gtgtgttcag ctcgggcggg cccgctcacc ggttgaaccc acttttgacc
      841 gctaatggcc ggcctctcgg cggctccacc gcggccgacc caattttgac cgattatcgg
      901 gcccgaattc ggcctgagaa ccgatcaaaa ttgggttggt cgctagcgag ggctcctgca
      961 ccgccccata gccagcaaaa agtgggttga accgttggcg gcgcccgcaa cggagtatcg
     1021 gctgcgcgcg tgtctagcct ccagccagcc ctacccgttc gccgaagcgg acggctgggc
     1081 ggccgtccgg gtgccctacg tcggcggcga actggccgcg gagttgatcc tgccgccggc
     1141 cggcaccgcc ccgtccgcga tcagcaccga caccttggcg aagctgcacc aggcgctggc
     1201 cgccagcacg tccgaaccgg tggagctctg gctgcccacc ctcgatctgg cgcccaagcc
     1261 gctcgacctt cgcggggtgc tcggcgatcg ggcgccgtcg gtgctctgcg gcgcgccgaa
     1321 caccgacctg tccgggatcg gtccgcagga tctgtgcgtc agccaggccg tgcagcaggc
     1381 ggtcctgcag ctggacgagg aaggcaccaa ggcggctgcg gtgaccgaga tcggcgccgt
     1441 ggcgtccggc gcgccggccc agcccgagca caagctgcac ctggaccgcc cgttcctgct
     1501 gcagatctcg gccagcagca ccggttggcc gctgttcctg gccgccgtga ccgacccccg
     1561 ccactgagtc gaggaatcgc cagagtcagg cggagacctc gcggcgagtc ggcactgaat
     1621 gcagcgggac ggtcttcacc ggctcgccgg tgaggacggc gacgatcgcg tcctcggtga
     1681 cctgcaggta gctgcgtccg tcgggagcga tggagttggc gtcgaagtag tggtccttga
     1741 cgttgtgctg catcgggccg aggtcaatga tctcgatccg gccgtccttc agcgcgttcg
     1801 tccacggcga gaagaccgcg tgcggccagc ggccggcgaa gaggatgccg ccgagttttt
     1861 ccagcgggtc cttggagccg cgcaggtgcc agagcttttg aatccggtcc aggccgtagt
     1921 catcggccat catgccgcca actgagatca cctgcagcgg gatccgtccg cccatgccca
     1981 ggtaccaggc cgcaccgatc gagatctggg cgccgccgct ccagccgatc agcgtgaccg
     2041 gcaagccgct gcccatcggg tagccgtggc ggcgcagcgc acgcatcact tcctgcgcgg
     2101 tgccgacgtt gtacgccggc ccgtagcggc ggtcggcgca gacgaacagc tgcatcgcgt
     2161 tgcgggcatt gatcagcatg ccggccaggg tctccgggtt cttgaagcga agcttctcca
     2221 cccaacgcca gaccgcggcg gtcggacgct gcgcggtgag cccgttgttc gagaccgagt
     2281 acgggaagac gtcgtggatc accttcagcc gggtgagccg cgaggagagg ttctggatca
     2341 gcggcaactc ctccggcggg acggagtcgc ccccgaccgc gccgatgccg gacaggtaga
     2401 cgacgtagcc gtcgtaggcg gagtcgtccg gcccggagtc gatggcggcc aattcctgga
     2461 cggtggctgc ggcctcgtcc gcgccctccc gcgcccacca gcccagcgat tccagcggcg
     2521 cgaaggtcag ggcgaggagc accagcagac cgaggccgat cagcacgttc accaggatca
     2581 tgaccgcgcc tccggatcga agcgctgccc gagcggcagg aacaagtgcc cggaaagcag
     2641 gtcgctgccg gtgatcatcg aaggctggcc cgagaccagc cgccagatcc agtcgccgat
     2701 ccatctgatc ggcaccgcga acgcccggga cagcacctgc atgcacagcc aggagatcag
     2761 ggtgatccac agcgccgtcc agtgatcggt ccggaacacc acgtcgacga tcacccacag
     2821 cgccaccatg ccccagacgt gcaggaagcc ggcgatcgcc ggcccggcgt acgggaccag
     2881 cacgaacacc ccgaagacca gcggcgcggt agccagcagg acggcggtga ccaccgcggt
     2941 gaaccctgga tcgaacccag agatcagcgg gccgatgacg tagatgaccg cggcctgcac
     3001 caggtagagc agcaccatgc cgatgccgtt cagcgcgagc gtcaacgcga accgccagcc
     3061 gcgcagccgg ttgaagaaga tgaccacggc gtcgcccagc agcgtggaga tgccggcgat
     3121 ggcagccacc gcgaccgcga tcccaagtcg ctgcggatgc tgcgcgatcc agtcggcgtt
     3181 gcccacgtcc aggccgagga cgccgacgat cacgcgtccg accgcgccga ggaactcaag
     3241 gatgcccatg ccggcctcca gccacttcgg tactgctcga tcccggtcgg gagcgggcct
     3301 cgccaacctc ggccagcatc cgcgggtgct gcggatccgc aggctatcag cgcgggttcg
     3361 gcaaggggga ataccgcatt gtgaccgtca tcgtcgtgcg gtggcccgcg ggcataacgt
     3421 agccgcgctg tgcccgcccg caatgccgcg gcctaccgga gaaggacccc catgagcttg
     3481 ttgcgcacca agtcgatcga acagtctatc gccgataccg acgagcccga gtaccagctg
     3541 aaacgatccc tttccgcgct cgacctgaca gtcttcgggg tgggcgtggt gatcggcgcg
     3601 ggcatcttca ccctcaccgg acgcgcggcg cacaacgtgg ccggcccgtc catcgtgatc
     3661 agcttcgtgg tggccgcgat ctgctgcgcg ctcgccgcga tgtgctacgc cgagttcgcg
     3721 tccaccgtcc cggtctccgg ctcggcctac acgttctcgt acgcgtccct gggcgagatc
     3781 ttcgcctgga tcatcggctg ggacctgatt ctggagatgt tcctcggcgc gtccgtcgtg
     3841 gcgcagggct ggagcgcgta tctgggcgtg ttcctggaga agatcggcgt cgtgctgcca
     3901 ccggtgatca gctacggcgg cctggtggac ctgccggcga tcctgctcgt ggtggtactc
     3961 ggcgggctgg tgaccatcgg catcaaggag tcgctgcggg tcaacctggc cctagtggcg
     4021 ttgaagctct tcatcgtgct cttcgtgatc gtcgccggca tcgggttcat caacccggcc
     4081 aactactcac ccttcgtacc gcccaccgca cccaccgaag ccgcgaccgg ctggacgcag
     4141 ccgctgctgc agttcctcac cggcggagcg ccgaccgcct tcggcatcgg cggcatcttc
     4201 gcgggtgcgg ccctggtctt cttcgcctac atcggcttcg acgtggtggc caccaccgcc
     4261 gaggagacca agaacccgca gcgcgacctg ccgatcggca tcatcgcgtc cctggcgatc
     4321 tgcaccgtgc tctactgcgc cgtcgcgctc gtggtgaccg gcatggtgcc cttcgaccag
     4381 ctcgacccga aggccgccct ggccaacgcg ttcgccttcc acggccaggc ctggatggcc
     4441 accctgatct cggcgggtgc ggtggccggc ctgaccacgg tggtgctcac cctgatgatc
     4501 ggcgccaccc ggctgatctt cgccatgtgc cgggacgcgt tgctgccgat gaacctggcc
     4561 aaggtgcatc cgaagtaccg gacgccgtgg gtgatcacga tcatcgtgac cgtcgcggtc
     4621 gctctcgtgg ccggcctgac cccggtgggc gtgctggagg agatggtgaa catcggcacc
     4681 ctgtcggcct tcgtgctggt gtcgatcggc gtgatcgtgc tgcgcaagaa gcgtcccgat
     4741 ctgaagcggt cgttcaaggt gccgttctcg ccgtggctgc cgatcgcgtc cgcactgatc
     4801 tgcggctacc tgatgctgaa cctttcggtg gagacctggc tgcgcttcct gatctggctg
     4861 gtgctcggct tcgtggtcta cttcgcctac tcccggtccc ggtcgcgcct ggccaccggc
     4921 gtaggcatcc gtccggacat gctggacgcg atggggcacg gccacgcccc ccaggacccc
     4981 gacgaccagc gctaaccccg ccacccagcc caccacgcac cgcccaccgc ccaccgcccg
     5041 ccgcggcgcc ggccacccgg acgcggccgg gccacccggg cgactggggc gatttgccgc
     5101 cgaagagtgc atttgccgcc gatatttcgg cggcaaatgc cactcttggc ggcggattcg
     5161 ggagaagcgc ggacgcgcgg ggcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5281 nnnggcggca aatgccactc ttggcggcgg attcgggaga agcgcggacg cgcggggcgg
     5341 gcggacgcgc gggagctacg gacggtggcg gcgctgctgg ccgtcagcta gggcggtggc
     5401 gattcgattg cggaaggccc gacgatcgtt gagatctggc caggtccagc ggacgaccac
     5461 ccagccggcg gcttcgatgg cggcctgacg ccgcttctcc gccatcacag cggcggctac
     5521 ctcgcgctcc gttccggtgt acttcacggc tccgtcgaac tcgcccacca ctccgtactc
     5581 cagccaggcg aagtcggttc ggcccagcca gcgtccgtag tcatcgaata cctcgaactg
     5641 gagcaccggc accggcagcc ccacttcggc catccgcact cggctgatcg attcggcgac
     5701 gctctccgcg cgacgatcgg cgaactcaag cgcgcgacgc agcacgcccg cgccgctggt
     5761 gtatctggca gccgcggaca tgtccgccag caactgcgga tcggccttcg cgtgcaacgc
     5821 ggcatccaga atcgcgaccg cccgctcgta gctgaccgat cgagccagat cgaccgccgt
     5881 ccgctccagg ctggtgacgc ggtagccgtc cagctcgatc aggtcggcgg agtccagccg
     5941 agcacagcgg acgtgtagtt ggttggtccg agcaccatgt ccaccgctcg cccgggtgat
     6001 cgaaacgcgg tgaagcatcg gcttccagac cggcaatccg tggagcaccg cggccgagcc
     6061 gtggctcaac acagcgttgg aagcgaggag cggccaggta ccggcgacga gctgccggtg
     6121 cttggcgagg tcgtccttcg gcgcctccgt ggcccacgcg ccatgacgta ccagcgtgcg
     6181 ctcgccgagt tcgagcgacc tggccagctg ccgctggttg gtcccagccg cccgcagctg
     6241 gctcgtccgc agaacatcca tgccgctatc gcagcccaag ccggacgcgt ccgccagagc
     6301 ggacaacaca ttgacaacaa tcggacaaca ccttctcgcc gggcaaccct ggccgcgtcc
     6361 gcgcaattcg ccgccgaagc acgcatttgc cgtggatatt tcggcggcaa atgccacttt
     6421 cggcggcgaa tccggcggga gagaccggcg gacgggacgg gagcggacga cggacgggac
     6481 gggcgagggc ggcggccggg caggcagcgg acgcaccggc ggcgtaccgg gtggggcgcg
     6541 aaccggtaga atcgacgccg aactcgacgc cgaggtcggc ggctcgccga caaatcggcg
     6601 ctctctagcc aaatcggaga ctcaatgccc gtacgcgccg acctgcgcaa tgtcgcgatc
     6661 atcgcccacg tcgaccacgg caagaccacg ctggtggacg ccatgctgtg gcaatcgggc
     6721 gccttccgcg agaaccagga cgtgaacacc cgggtcatgg actcgatgga cctggagcgg
     6781 gagaagggca tcacgatcct ggccaagaac accgccgtag accacgttct ccaagacggc
     6841 tccacggtca cgatcaacat catcgacacc cccggccacg ccgatttcgg tggcgaggtg
     6901 gagcgcggcc tggaaatggt ggacggcgtt ctgctgctgg tggacgcctc cgagggcccg
     6961 ctcccccaga cccgcttcgt cctgcggaag gcgctggcaa agaacctgcc gatcatcgtg
     7021 gtgatcaaca aggtcgaccg cgccgacgcc cgcatcgccg aggtggtcga cgaggtctac
     7081 gcgctcttcc tcgacctgct cgacgacgat gaggcccacc acctcgactt cccgatcatc
     7141 tactgcgccg cgaaagccgg acgcgcctcg ctcacccagc ccgcggacgc gtccctgccg
     7201 gattcggcga acctggagcc gctgttccag ctgatcatgg acacgatccc ggcgccgctc
     7261 tacaccgagg gtgccccgct gcaggcgcac gtgaccaacc tggacgcgtc cccctacctc
     7321 ggccggctgg cgctgtgccg catcgtcgag ggcaccctca cccgcggcca gacggtggcc
     7381 tggtgccggc acgacggcag catcaccaac gtccggctct ccgagctgct gatcaccaag
     7441 gccctcgaac gggtacccgc ggagtcggcg ggccccggtg acatcgtggc gatcgccggc
     7501 attcccgaaa tcaccatcgg tgagaccctg gctgacccca acgatccgaa gccgctgccg
     7561 ctgatccacg tcgacgagcc gtcgatctcg atgaccatcg gcatcaacac ctcacccctg
     7621 gccggcaagg tgggcaagct gctcaccgct cgcctggtga agaaccggct cgacaccgaa
     7681 ctggtcggca acgtctcgat ccgggtgctg aacaccgacc gtccggacac ctgggaggtg
     7741 cagggacgcg gtgagctgca gttggcagtg ctggtcgaga tgatgcgccg cgagggcttc
     7801 gagctgaccg tcggcaagcc gcaggtgctg gtgcgcgaga tcgacggcaa gctgcacgag
     7861 ccggtcgaac ggctcacggt cgacgtgccc gaggagcacg tcggcacgat cagccagctg
     7921 atgggcaccc gccgcgcgcg gatggagcac atggtcaacc acggcaccgg ctgggtgcgg
     7981 gtcgaatacg tgatcccggc gcgtggcctg atcggcttcc gcaccgagtt cctcaccgag
     8041 acccgcggca ccggcatcat gcaccacgtc ttcgagggtt acgagcgctg gtcgggcgag
     8101 atcaagaccc gtcagtccgg ctcgctggtc gccgaccgta ccggcgtggt caccggctat
     8161 gcgctgttca acctgcagga gcgcggcacc ctgttcgtcg cgcccggcga cgaggtgtac
     8221 gagggcatga tcgtgggcga gaacccgcgt cccgatgaca tggacgtgaa cccgaccaag
     8281 gagaagaagc tgaccaacgt ccgctcctcc accggggacg aactggagcg cctgatcccg
     8341 ccgcggctga tgagcctgga gcagtcgctg gaattctgcc gcgaggagga gtgcctcgag
     8401 gtgacgccga agaacgtccg catccgcaag gtgaccctgg acgcgaacga gcgggccaag
     8461 cagcgcaacc gcgccaagaa ggcctgagtc ggattcgcgg gcgcagcgac ctgccagtcg
     8521 ggacggccgt acgcccggtt gacgtttgcc accgagaccg gcgtttgcca ccgccatctc
     8581 ggtccgccct actgccccgc cgctcgatcc tgagtcgcac cggcgaaact agcgcttgcc
     8641 agggaatctt ccccggcaag ggtcagtttc gccggcttgg caatgtgcgc ggcaaccggc
     8701 agatccgggc cacagctttg gtgtcggtag caggcgccgg tttcggcagc aggcgtcggt
     8761 ttcggaggag gcgtcgcgcg acttgggtgt ccgagccggg tcggccagac tcgggctggt
     8821 ctcgacgctg ccgttcggag tgaccgaagc cgagcgttag gctcatccca tgtcgttacc
     8881 ggtgctggat gccaccgagc agcgggttct gggttcactg ctggaaaagc agcgtacggt
     8941 ccccgccagc tacccccttt cactgaacgc cctgcggctg gcctgcaatc agacgtccag
     9001 ccgtgatccg gtcgtcgact actccgagcg gacgattcag gacgccgccc gcgcgctcaa
     9061 ggaccgcggc ctgctgcggc tggtctgggc cggcgcaggg tcgcgcgtag tgaagtacca
     9121 ccagttgctg gatgaggtgc tgccactgga cgaggccgac cgggccctgc tcaccgtcct
     9181 gctgttgcgc gggccgcaat cccccggtga gctgaagacc cgcaccgaac gcctgcatgg
     9241 tttcgccgac aaagccgagg tgtcggagcg cctcgaagcg atggctgccg ccgagccacc
     9301 gctggtgcgg gaattgtcgc tggtccgcgg ccagcaggat cgccgctggg tacacctgct
     9361 cggaccagta ccggtcggtg gcgcggacgc ggaggcgccg ccagccgtgg atcgcgaggc
     9421 agcgctggcc gagggcgctg ccgctcggga cgcgaaggtg atcgccgcct atgacgcggc
     9481 cgccgcggcg tacgccgccg agttcggcga ggagctgatc gagaagcctt tcgacatctg
     9541 gctgctggag cgggtggccg aggcggccga cggcccgatc atggatctgg gctgcggacc
     9601 ggggcatatc gcggccttcc tcgccgatac cggcgccgag gtgcacggcc tggacgcgtc
     9661 cagcgcgatg atcgagcagg ctcgctccgc ctatcccgat ctggatttcc aggtgggccg
     9721 gttccaccag ttgctgcgtc cgcggacggc ggcggcgtgg ggcgcgatca tcgcgtggta
     9781 cgccttcggt catctggccc cgtccgagct cggcgcggta ctgcggaccg cgtccggcac
     9841 cttgaacccg ggcggactgc tggcgctggc gttggaactc ggcgaccggg tgcggcacgc
     9901 tgattccctc tgcggcgtgg aagtcgacct cgactacgtc ctgcacgacc gccaacaggt
     9961 gctggccgcc ctggccgcgt ccgatctggt ggttgacgag tggtacgtgc gagcaccggt
    10021 tgggggcgag gccgatacca aacgcctata cgtgctggcg cgcaaggcgg gctgagattc
    10081 ggccctcccg ggcgaacggt ggctgagctt gacgaagccc ctcccaacgg cccatcgtca
    10141 ggctcaggga tcgttggggg gaggcctcag ggaacgtttg agggatgact cagagtcggc
    10201 cgaggaagct cactatcgcg ttcacgatct cacgttcggc gtcggtgcgg ctcacggccg
    10261 ggactccgtc cccggactgc ggaccgtagc ggccgaagaa gctgtgcacg ccgccgtcga
    10321 tctgcaacag ctttgaatcc ttcgggaggc gcctcagccc gtccgcgatc ttggcggcat
    10381 cggcgacatt gtcgaactcg cccttcaggc tcgctacctt gagcttcgcg gacgccaggc
    10441 tggccccgtc cggcgggtag gcggccatca gcaccagccc ggcgaccggc tcgcgtccgg
    10501 ccttcagttc gtcggccagg tattgcgcgg ccatcgcgcc gccgagcgaa tgcccggcca
    10561 gcaccacctt gcggccgtcg gcgtaccgct ggatcaaggc ggacgcacgg tcggtgtcgg
    10621 tcaccgcgag gtcgaaggag aactccggga tcagggtctc gtagccacga acggccagca
    10681 ttcgggccag ccactcgtac gcctgcggac gcaccagtcc gccggggtag atgatcacca
    10741 ggacgtcctt cgctgcggtg cccgcgggcg gcgcgacccg gatcagccgg ccgccggcgt
    10801 cctcgatcac cggcgttacc ccgatggtgg cctggtcagt gatcgcgtcc tggccgacga
    10861 tcagcggtgg tcggaagaga accggccaag cccagcccgc accgaagacc agcgccagcc
    10921 ccagcagtaa acccagccag cgaaaagctc gcttcatcgc acacctcccc cgcgaccatg
    10981 atgaggctcc tcggccgccc gcgtcagtcg aagtcgaggg ttgtgctgat cgaggtctcg
    11041 aaagccgacc ccagcagtgg gggcgacttc ccacaggttt gaacccactt ccggcaggtt
    11101 atggcggccg tcacagctgg ccgaaccgga ccaacccaaa ttctgcaggt tatggacggg
    11161 aattcggccc gcgtaacctg ctgtttttgg gttggcggga atctgcccct gaacagcccc
    11221 gccataacct gacgaaattg gcttgaacgc gcgagggtga cgccaacacc gtcgcagcgg
    11281 gcaaaccggt tcgggtcagc ggcccaaacc gggctgcggc cgccagtgca gcccagcgtc
    11341 cgggtcaacg atcgtctctt gggcggccgc gataccgtcg acgaccagtt cggcgtctgt
    11401 cggtaacgtc cgcttcagca gtgcgagccc gatcgggccg agttcgaaat gccgctcggc
    11461 agtgcccatg cgaccgacga cgcgcccgtc gagaaagacg tcggcgccca gttcgggcag
    11521 ccgctcggcg ctgccgtcca ggtgcagcag ggtcagccga cgcggcggcc ttccgaggtt
    11581 gtacacccgg gccaccgtct cctggcccgg gtagcagccc ttacgcaggt gcacggccgg
    11641 gccgaggcgg tcgtcgtcgg ggttagccag ttcgttgggg atcgtcttct catcggtgtc
    11701 gaggaagatc cgcggagtgc cggacgcgat ccgcagcgca tccatcgccc aggtgccggc
    11761 ccgaacctcg cccagagtcg cgtccacctg agcggacgca acgatcgccg ggccacccct
    11821 gggcggcagc accagggagt attcgtcgcc gagctcgatg atctccacgc gcgctgcgaa
    11881 caccatccgc cgtagccagg ccactaatga gtcgcgccga cctggctcgg tgtgcgccca
    11941 caacgtctcg ccgtcatcca ccgcgccgaa cacatggacc aggtgcccct gcggatcgag
    12001 gatgtaggcg acggtgggcg tacccgaggg cagcgaagca aagtcctggc tggtgatgtc
    12061 gttcagccac ttcagccggt cggcacccct gatcgcgaac agcggccgat gcgacaaatc
    12121 cacgccagcc gtgccggcca gcagcgcccg ctgctcgcgc aacggatcgc cgtagtgcca
    12181 gaccgcgccc gcgtccaggc cctcggtgag ccggacgctc acctgcgctc caaggtcgcc
    12241 cagatgtagg gctgcagctc gtgctcctcg gtggcgcgat cgaaggagaa cagcagcttg
    12301 ccctcgacgt tgccgtagag ccggcggccg gcggtgtagc tgaccttggc gtcgcgggtg
    12361 cgggcgactg cgtcagtgat caggtcgagg cggccgggct gcaacttgcc gaaccagatc
    12421 tcggcgaagc cgtccgggga gcacatcatc acgtccacgg tgccgtcggg gagcggacgc
    12481 cagaagccgg tttccatgaa cagcggcctg gtcgggctac cgtccgagtc cacctcgaac
    12541 gcctgggcca ggtagtgcag gaaatcctcg ccgttgtcgg cgaagtccac ctgaatgcga
    12601 tagccgacct tctcctcgcc gggccactgc cgaaagccgg tgccctccca gcggccgcgc
    12661 agccaggcga cgcccaccag cgcggggttc aggtcggccg gaatctcgaa agccatgttc
    12721 agtctccttt tcggctgcgc agcagggcaa acagccccag cccgcacagc acgacgagca
    12781 cgaggcaggc ggtgaacagc accacaccgg tcaccgaaag cggggtcagc acctgtgcag
    12841 cttactcagc cgcctgtgga tgaggggtcc gaggacgccg gttctccaca ggcggccgat
    12901 tgagctgacc ggattcgggc gcggttacgg aggactaggg catgcgtact ccaacaccat
    12961 ggctgctgcc aaccgaaccc accgggactc gacaactgct ggcgatcggc atcagcaagg
    13021 agatgctgat cacccagctc aggacgggtc gcctgttgcg actgcgccga ggcgtgtact
    13081 tgacccgctc agcctggccc gacgacggcg ccggccagca cctgctgctg gcccaagcag
    13141 aactcgtcgc gcatcccggc gccgtgctga gccaccagtc ggccgccgtt gcctggaatc
    13201 tgccagcgcc cggcttaccg aggtggcacg aacgctcacc ttcgatcacc ctgccggccg
    13261 gcggtgggca ccgaacgtcc agggggccta tcggctgtag cgggggcttc atcagccttc
    13321 cagatcgggt cagggtcacc ggcgatcgag acaggtatct gatctgccac cgaagtcggg
    13381 tttcgcccgt cgacctgctt ggattggctc gtgcgagcgg tggtgcaacg agtcagccgg
    13441 gccagggttg aggtcgacgg cgaggtcatt ggccggatcg aggatcccgg gctgctggtg
    13501 ctgatcgggg tcacccacac cgacacggag tcggtggccg acaagctggc cgacaagacc
    13561 tggcagctgc gcatcctcga aggtgagcgg tcctgcgccg aggccagtgc gccactgctg
    13621 gtggtgagcc agttcacgct gtacgccgac acccgcaagg ggcgacgccc gtcctggagc
    13681 gcagccgcac ccgggacggt cagcgaaccg ctcgtcaacg cctacatcgc ggccctgcgc
    13741 cggcgcggcg cacacgtcga gaccggacgc ttcggcgcca tgatggcggt taccagcacc
    13801 aatgccggcc cgatgacggt gctcctggaa gccgacgcct gatcgagcgc acgtgggtct
    13861 cgacgcagcc accaataaat acccccgagt ttggttacag cgctgaaacg agaggtcact
    13921 aaggttggcg ctggaatcgg cccgcgagac cgtggaggtg tcggatggcg caactgctcc
    13981 tggtgagcaa tgaggtcgcg cgtggcaccc cggcggagat ctccttgccg gtgctgtccc
    14041 tgctcgcgca cgacatccgt ggggtcgccg cggacaccct tgcgctaacc gagtcggccc
    14101 gcgctgatct ggtggtgctg gacggacgcg ccgatcttcc cggagcgcgc accatggccc
    14161 ggctgttcaa ctcctccggg tccaccgtgc cgctgctgct ggtgctcacc gagagcggcc
    14221 tggccgccgt cgccaccgac tggggcttgg cggacgtggt gctcgacacc gccgggccgg
    14281 ccgagttcga ggcccggatt cggctgctgg tcacgtccgc ggccaacgac ggactgatca
    14341 gcgccggcgg catcgtcatc gacgacgccg cctactccgc gatgctggac ggcgccccgc
    14401 tcgacctcac ctacaccgag ttcgaactgc tgaagtacct ggtgcagcac cccggccgcg
    14461 tgttcagccg ggagcacctg ctcgccgacg tctggggcta cgactactac ggcggcaccc
    14521 ggacggtgga cgtgcacgtg cgccggctgc gcgccaagct cggcccggaa cacgaggccc
    14581 tgatcggcac cgtccgcaac gtcggctacc gcttctcccc ggacggctcc gcgcgccgtt
    14641 gagtcccgcc gctaccctgc aaagatgccg accaatcgac tcagcgctgc ccaagccgaa
    14701 caggtgctcg acctggccgc ggccgcgacc gcgcacgatg gcgttgatcc gctcaacgag
    14761 gaggcgcggt tcgcgctgcg cgacgactcc gccgagcact acctgcacca cgtcggcgac
    14821 cgattggccg gttacctgat ttggctgccg ttgcatcaca ccgggcagtt ggtggtgcac
    14881 ccggagtttc ggcggcaagg gatcggacgg acgctggttg aacaagcgtt tggggtgatc
    14941 gacggggccc cggccggctc gaccacacgt ccggtggcga tttgggcgtt cggccatctg
    15001 cgcgcggcgc agggattcgc gtccgctctg gagctcagcc cggtgcgtgg gctgctgatc
    15061 atggatcgcc cactcgccga accggtcaca ccgcacgtcc cggacgcact gcagctacgc
    15121 ggcttcgagc tggcggacgc ggccgcggtg ctcaccgtga acgcggccgc cttcgctcat
    15181 caccccgagc agggcagcct ggacgaggcc ggcctgcacg cccgcatggc cgagccctgg
    15241 ttcgacccgt ccggcctgat cctcggcttc gatgcggacg gtctcgccgg cttccattgg
    15301 accaagcgga tggatgcccg aaccggcgag gtgtacgtca tggcggttgc ccccagagcc
    15361 cagggacgcg gctacggcaa ggccctgctg aacgccgggc tggcccacct acaacgatcg
    15421 ggatgtgacc gcgtcctgct gtacgtcgac atggcggatc aggtagcggt aagaatgtac
    15481 gaaagcgccg gattccgagt cacccaccgc gacgtgctcc tggcgccgaa gccgaaggag
    15541 caacaatgac ggtcgaggaa caaactgtcg cagcagacgc cgccccgacc ccacccgacc
    15601 ttccggccga ccgcttctcc gaccgcgagc tgtcctggct ggcgttcaac aaccgcgtgc
    15661 tcgacctggc caaggacgcc aagcgggtgc cactgctcga acgcgccaag tttctggcca
    15721 tcttcgcctc caatctggac gagttcttca tggtgcgggt ggccggcctg aagcgccgga
    15781 tggctgccgg ggtcgcggtg accagcaact ccgggctgat gccccgcgag ttgcacgacg
    15841 ccatcctcac ccgcactcgc gagctagtgg tcgaccacgc ccaggtgttc gccgaggaca
    15901 tccggccggc gctggcccag cacggcatcg agatcctgcg ctgggacgag ctcacgtccg
    15961 atgagcgcga gaagatgacc gcgctgttcg agaaccggat cttcccggtg ctgaccccgc
    16021 tggcggtcga cccgtcccac ccgttcccgt acatctccgg gctgagcgtg aacctggctg
    16081 tgctgctgcg gaacccggcc accggcgtcc gccagttcgc ccgggtcaag gtgccgacga
    16141 tcctcagccg cttcgttccg ctcggcggcg gacgcttcgt cccgctggag gaagtgatcg
    16201 ctcgccatct cggccagctg ttctccggca tggagatcct cggccactcc accttccggg
    16261 tgacccgcaa cgaggacgtc gaggtcgaag aggacgatgc cgagaacctg ctgttcgcgc
    16321 tggagaagga gttgctgcgc cgcaagatcg gacgcccgcc ggtgcggctc gaagtcgaag
    16381 aggacatcga cgaccggatg ctgcagctgc tgatcagcga actcgacatc gccgagaagg
    16441 aggtcttccg gatgcccagc ccgctggacc tgcgcgggct gttcgcgatc gccgacctcg
    16501 atcgggagtc gctgctctac ccgtccttcc tgccgaagac gcacccggaa ctggccgagg
    16561 tggagacgtc ccggcccgcc gacctgttcg cggcgctgaa gagccgtgaa gtactgctgc
    16621 atcatccgta cgactcgttc gccaccagcg tgcagcgctt catcgagcag gcggcagccg
    16681 atccggccgt cctggcgatc aagcagaccc tctaccgtac gtccggcgac tcgccaatca
    16741 tcgacgccct ggtcgaggcc gcgcaggcgg gcaagcaggt gctggcgctg gtcgagatca
    16801 aggcgcgctt cgacgagcag gccaacatcg cctgggcgcg caagctggag aaggcgggct
    16861 gccacgtggt ctacggcatg ctcgggctga agacgcactg caagctgtcg ctggtggtgc
    16921 gcgaggagga gaccgggctg cgtcgctacg ttcacatcgg caccggcaac tacaacccga
    16981 agaccgcccg gatgtacgag gacatgggcc tgctgacttc gaacaacatc atcaccgatg
    17041 acgtcgccag gttgttcaac cacctctccg gcatggcgca ggagacccac tacaagcggc
    17101 tgctggtggc gccgcacggg gtccgccgcg ggctgatcga gaacatcaac cacgagatcg
    17161 agaaccatcg cgccgggcta cccgcccggg tgcggatcaa ggtgaactcg atcgtggacg
    17221 aggcggtgac tgacgcgatg taccgcgcgt cccaggccgg cgtgccggtg gacctgtggg
    17281 tgcgcggtat ctgttcggtg cgtccgggcg tgccggggct cagcgagaac atccgggtgc
    17341 gttcgatcct cggccggttc ctcgagcaca gccggttgta ctggttcgcc aacgggggca
    17401 atcccacggt cggcatcggc tcgtccgacc tgatgcatcg caacctggat cgccgcgtcg
    17461 aggtgatcgt cgggctgaac aaccccaagc acatcgacca gatcgaaggg ctgttcgaac
    17521 tggcctttga cgaggggacg gcgtcctggt ggctggaggg cgacacctgg acccagcgga
    17581 cgctggacgc agacggcgag ccgctgctcg acatccagga ccacctgatc gccaccatgg
    17641 gccaacgtcg ggcggtcgag cgctagacga tggcgaacac tcgcgtctcg aagcagatca
    17701 ctgcggccgg cacggtcgtg ctccgggaga acccggacgg ccagaccgag gtactgctgg
    17761 tgcaccggcc gcggtaccag gactggagcc tgcccaaggg gaagctcgat ccggacgaat
    17821 acctggtcgg ctgtgcagcc cgcgagaccc gtgaggaggc cggggtcaac gtgcggctcg
    17881 gcattccact ggacccgatc cagtacccgg tcagcgcagg caccaagacg gtcttctact
    17941 ggcgatcacg ggtcgtcggc aacggccggc agaaggccaa cggcgaggtg gacgacgccg
    18001 cgtggttcag cgtccgcgag gcgctcgacc tggttaccta cgccgacgag cgtccgctga
    18061 tccggcaggc ggtcgccctg ccggactcga cgccgctgtt gctgatccgg cacggcaagg
    18121 cgctgccgcg cagcgactgg gacgggatcg attcggagcg tccgctggat gaacgcggtc
    18181 ggctgcagag ccagcggttg acgccactac tggacgccta cggggtcggc cggctggttt
    18241 cgtccacgtc ggcgcgctgc ctgcagaccc tcctgccgca ctcgcacgcc agcggcctgg
    18301 tgctggaggc cgaacccgcc ctcagcgagg agcgcttcgc taccgagccc aaggcagtca
    18361 ctccactgct caaacgcatt gcgaagcagg ctgccgagac cgggaagccg gcggcgatct
    18421 gcggtcaccg tccggtgctg cccacgatgc tcgccgcgct caagctagcg ccgcgcagcc
    18481 tgcagcccgg ttcgaccctg gtcgtccact tggccccgga cgcgtccgtg ttggccgtgg
    18541 agttgcacaa gcctgaggtc tgaccacggg agccggcaac tgttgccaga gtcagcgcct
    18601 cggcgcggcc gtacttcttc gtagtacgaa cttcggcggt agcaccaaat gctgggtgcg
    18661 ggccttgccg gtctcgatca gctgcaccaa cagctccacc gagagctgcc cgatctcgtc
    18721 gaagtcctgg cggatcgtgg tcagtccggg ccagaagaac tcggcctcgg gggtgtcgtc
    18781 gaagccgacc acgctgagct cgtccggcac ccgcaggcca agttcatgcg ccgcgtacag
    18841 aactccgagc gccatggcgt cgttggccac gaagatcgcg gtgacctccg gatcgcggag
    18901 cagctcgcgt ccagcctggt agccggacgc cgccgaccag tcaccgcgct ggatcggctt
    18961 gatcggcgcg ccggcggacg tcagcgcctc ctgccaacca gtgacccgtt cggtgccctc
    19021 gtaccagagg ttcggacccg ccaggtgcca gaccgtacgg tgcccgagct tcaacaggtg
    19081 ctcggtagcc agccgggcgc ccaggcgctg gtcgaccgag accaccgggt agttgcccgg
    19141 cgttccggca tggatcgcca ccatcggcgg caggtccgcc ggcggctcga cctcgtgcac
    19201 ctcggagatg agcggctgga gcaggatcac tccgtccgac gagcgcgact gcagcaggtg
    19261 gacaccctgg gccaccgcgt tgcgatccgg atcgtccagg tgcgcgaccg tgacgctgta
    19321 gccgcggtcg cgcgccgcca gctcgatgcc gcgcagggcg gctcgcgggc cgaacagcgt
    19381 ggtgtcgacg gtgagcacac cgatgctccg ggagcgtcca ctggccaggc cacgggccat
    19441 gatgtttggc cggtaatcga gcgagcgcat cgccgcgcgg acgcgttcgc gggtgtcggt
    19501 cgcgactttc gcgtgaccat tgatcacgcg ggagacggtc atggtggata ctccagccag
    19561 ccgagccacg tccgtcagtg tcggcggacg atagaccgca gtgccgtccc cagcgacttc
    19621 acccatgggt gttagcgtat tacactccag cccgcgggtt tcagaaaccg cggccgaaga
    19681 cggacgtgcc gcccgagatc aaagcggaaa tctggcccgc tattcacctt gcgttcaccc
    19741 ggcagtcccg gagcggtaag ccggcccacc tagggtcagc agcggagcac aaccgctccc
    19801 aaaccaagac gctaaggaaa ctccgtgaag atcaagccga tcgccgccgt gctggccacc
    19861 gccaccgcgt tcactctcgt gggctgcgca gcgaatgagg ccgccggcac tggtggtacc
    19921 tcatccgccg ccgcccagac cctcaccggc aaaggcgcca gctcgatgaa ggcagcccag
    19981 gagaagtggg tcgccgactt ccagaccgcc cacaccggca tcaccgtcaa ctactcgccc
    20041 gaagggtcca gcgccgggcg ctcggccttc accgccgggg ccgtccagtt cgccggctcc
    20101 gaccgtccgc tcaaggacga ggagatgggc gcaggcaagt tcgccacctg cgcggccgac
    20161 tccaacgccc tgaacctgcc ggtctacgtc tcgccgatcg cgatcatctt caagctggac
    20221 ggcatcgaca agctcagcct cgacccggcc accgcggcca agatcttcgc cggtcagatc
    20281 accaagtgga acgacccggc gatcgtcgcg ctcaacccgg acgccaagct ccccgacagc
    20341 aacatcaccg ccgtccaccg cgcggacggc tcgggcacca ccaacaactt caccgacacc
    20401 ctgcaccagc tggctccgga ggtttggacg gaatccgccg ccgacgactg gccggccgcg
    20461 ttcggcggtg aggcagccac cggcacgtcc ggcgtggtca gcgcggtttc cggcggacag
    20521 ggcacgatcg ggtacgcgga cgcgtccgcg gccaagaacg tgggcatcgc cagcctggcg
    20581 gtgaacggca agtcgctgga gccgtccgcc gaggcggccg cggccgtgat cgaccactcc
    20641 cagacggtcg ccggacgcgg cgagcacgac ctcgcctact ccctcgaccg cacctccgag
    20701 ggggtctacc ctgcggtgct ggtgtcgtac gcgatcgtct gcgagaccta caaggacgcg
    20761 gccacggcca agctggtgaa ggactacatc ggctacatcg cctccgccga gggtcagcag
    20821 gcggccgcga ccgcagccgg atccgcgccg ctttcggccg cgatccaggc caaggtcaag
    20881 gcagcggtgg attcaatcaa gtgacgtcag ccctcgacgg cgccggtggt gggcccaccg
    20941 gcgccgtccg gacgcccggg catagccttg ggtcaatcgc cagaaagcag gcagacgaga
    21001 tgacccaccc gctgtccacg gattccggcc ccggacccgc gggcgccccg gaggcagccc
    21061 tggcggccga tcccaagcgg ttcggcgacc gggtgttccg cgggttcgcc accggcgccg
    21121 gcgtgctcat cctggtgatc ctggcgctgg tcgccggctt cctgttgatc cagggtgcgc
    21181 cggcgttatt cgccgacccg gaccggctcg cttcgcaggg ctacggcgag ttcgcgtcct
    21241 gggtgctgcc ctacgcgttc ggcacggtct gggcggctgt gctcgcgctg gcgatggcgg
    21301 tgccggtcgc aatcgggatc gccctgttca tctcgcactt cgccccacgc cggatcgcgc
    21361 agccgctcgg ctacctgatc gacctgctgg cggcggtccc gtccgtggtg ttcggcctgt
    21421 ggggctcgac cctgctggcc cgcgcgctga tcccgttgta cggctggctc aacgccaacc
    21481 tcggcttcat ccccttcttt gccggcatca cgtccggcac cgggcgcacc atcctgaccg
    21541 cggccatcgt gctcgcgacg atggtgctgc cgatcatcac cagcctctgc cgcgaggtct
    21601 tcctgcagac cccgcggctg cacgaggagg ccgcgctggc actcggcgcc acccgctggg
    21661 agatgatcaa gatggccgtc ctgccgttcg cgcgtccggg catcatcgcg gcggtcatgc
    21721 tggggctggg ccgcgcactc ggcgagacca tggccgtctc aatggtgatg tcgggcggac
    21781 gcaacatcac gttcgaggtg ctcaccagcc agaaccccaa caccatcgcg gccaccatcg
    21841 cgcagaactt cgccgaagcg tacgggctga agaccaacca gttgatcgcc gccggcctgg
    21901 tgctgttcct gatcacgctg gccgtgaaca tgctcgcccg ctggattgtt tcccgtcgcg
    21961 ccgaatactc cggagccaac tgatgagcac gttgaatccg tccgcagggc cgctaggggc
    22021 tcgaccaacc ggcggcaccg gacccctgct gggcggcgac acactcacct ccggacggct
    22081 gcccaaggcg accacctggg tcgcgctggt ggccagtgcg atcgtgggct tcgcgatctc
    22141 ggtcctggcc accggcgcgg ccaacgccgg actcaccgtg ctgttcggct acgtgctctt
    22201 cctgctgatc atggaagtcg ccagccgagt ggtggaaggg ccgcggcagg cgtccgatcg
    22261 tagagccggc aacctgatca tcggcgcgtt cctgctggcg ctgctgccgc tgggatcggt
    22321 cacctgggag gccgtcagca acggcatggc ccgcttcgac ccgtccttct tcaccaactc
    22381 catgcgcaac gtggtcggcg agggcggcgg tggcgtccat gccatctacg gcaccctcta
    22441 cgtcactggc atggccagcc tgatctccgt cccaatcggc ctgctgaccg cgatctacct
    22501 cgccgagtac ggcgcgggca atcggctggc caagctgatc accttcttcg tggacgtgat
    22561 gaccggcgtc ccgtccatcg tcgccggcct gttcgcgtac tcgctgatgg ccctgctggt
    22621 cgggccgggg gcagtgttcg gcttctccgg cagcgtggcg ctttcggtgc tgatgatccc
    22681 ggtcgtggtc cgctcgtgcg aggaactgct gcggttggtg ccgaacgagt tgcgcgaggc
    22741 cgcgtacgcg ctcggggtgc ccaagtgggt gaccatcgtg cggatcgtga tccccaccgc
    22801 gatctcgggc ctggtctcag gggtgatcct tgcgatcgcc cgggtgatcg gcgagaccgc
    22861 gccgctgatg atcgctgccg gattcacggc ctcgctgaac aacaacctat tcgccaaccc
    22921 gatgatgacc ctgccggtgt tcgtctacga cgcgtacgcg caccccggcg tggagcccgc
    22981 accctacctg gaacgggcct ggacgggtgc cctcaccctg atcctgatcg tgatgctgct
    23041 caacctgatc ggccggctga tcgccgcccg cttcgccccg aagaccggac gctgagagag
    23101 aacctgaatg tccaagcgga ttgaagtcac cgacctgaac gtgtactacg gctccttccg
    23161 ggccgtcgag gacgtgacca tgaccatcga accgcggacg gtgaccgcgt tcatcggccc
    23221 gtccggctgt ggcaagtcga cctttctgcg gacgctgaac cgcatgcatg aggtgattcc
    23281 gggcgctcgg gtcgagggcg aggtgctgct cgatggggag aacctgtacg gcaccggcgt
    23341 cgacccggtg cgggtgcgtc ggcaggtggg gatggtgttc cagcggccca acccgttccc
    23401 gacgatgtcg atccgggaga acgtgctggc cgggatcagg ctgaacttcg ccaagatgcc
    23461 gaaggccgcg caggacgagc tggtcgaacg ctcgctgcgc aacgccaacc tgtgggaaga
    23521 ggtgaagaac cggctcgacc tgcccggttc gggcctgtcg ggcggtcagc agcagcggct
    23581 ctgcatcgcc agggcgatcg cgatggcacc cgaggtgctg ctgatggatg agccctgctc
    23641 agcgctcgac ccgatctcca cgctcgcgat cgaggacctg atcagcgaac tcaaggagaa
    23701 gtacacgatc gtgatcgtga cccacaacat gcagcaggcc gcccgggtct cggacgtgac
    23761 cgctttcttc aacatcgccg gcaccggctc ccccggccgc ctgatcgagt ccggccctac
    23821 cgacaagatc ttctccgctc ccgacatcgc cgccaccgag gactacatct ccggccgctt
    23881 cggctgagca cccccgtcca cgactccagg gtcagctccc gtggctggct aggcttagcg
    23941 cgtgagcaac gctggttggt atccggaccc gggcggccag cccggcatgt accgctattg
    24001 gacgggcacc gagtggacgt ccgccatcac ggccaatccg gccggcaccc aaccaccgtc
    24061 ctattcgtcg gggcagccgg ctggtgtccc tggccaggga cgcaagcgcg gcgcgaccgg
    24121 ctggtggctg ggtggcctcg cggctctggc cgctgtggtg gtggcgatct ggttcgtggc
    24181 gcagtcgttc ggcaccaccg gcgggatcct cggcggcgac gatccgacca ctccgggcgg
    24241 tggcgccacc cagttgatct gcccacccgg cgacaacagc accgcgtcgg ctccgcctgc
    24301 cggtgagccg ggctggacca ccggtggacg gctctctttc ccaacgctcg gcgacccgtg
    24361 gacaaccggg cacgactacg aggtgcccta cggctcggtg gccaccgtgc agacggtgct
    24421 cgaccaggac aattacgacg gtcggggcaa ctcgtgggtg gccagcgtgc tggtcagcga
    24481 cctctacgtc ggcgacggct tcgccagccc caaacagggc gctgagatcg tcctgaagtg
    24541 cgtgctgggc aagtactacg cggacacgat cgtcacccag aacccgatca gcagcgcaaa
    24601 gcatgacgtg gacggccaca gcgggtggct gatcgagacc gacctcagct tcagcatctc
    24661 caacctcaac gcgaccagcg aacgcgtcct gctgttggtg gtgcagaccg gcaccgagga
    24721 gtacggcctg ttctatgcgt ccgtcccgaa caccctcgac cacctgatgc cggacgcgcg
    24781 tcaggcgttg gccaacctac gagtggaagc ctgaccgcaa gcgttcccgg agcctgacga
    24841 cgggcgtgag cctagagatg gttcttgagc ctgacgtagg ggcgagctca gccgcaggac
    24901 acgatgccga ggccctgctt cattcgcagc acccgggcaa cagatcgcgt gacctgctcg
    24961 gcgaaggctt catccttgcc ggcttcgacc ttgaccgcgt ccaccatcgg gccgaccagc
    25021 gcgggcgagg cgttgatcac cacgtccccg ccggcggaga ggaagcgcag cgcgcgctgc
    25081 cctgcgggga tcttggacac cgagactgcg gcgccgaggt cgtcactgat caccacgccg
    25141 tcgtagtccc agtcccgcaa caggccgatc accttcggcg agaacacggc ctggttcttg
    25201 gcatcgatct tggaatagcg agccgaggag atcatcaccg cggccacgcc ggtctcgatg
    25261 gcggttcgga acgggagcag cgaggcgctg tcggcagtgg tcacatcgtc caccacaccg
    25321 gacgcgtgat cagtgttgcc gaccacctcg ccgagtcctg gaaagtgctt caccgtggta
    25381 ccgacagcag cggcggagta gccgttgatc accgcccgca ctttctccgc aacgagttcg
    25441 ggggccgacc cgaagccccg attcagcttg ccgatcgggg cgttcgactt caccttcttg
    25501 gccggtacta cgtcagccac cggggcgagg ttcagctgga cgccggcctc gatcaactcg
    25561 acgccccagt tgcgggctcg cgcctggagg ctgtccaacg gcaactcggc ctgcttggtc
    25621 gctgaaggaa tcttgcggaa cccggtgccg gtgagccgct gcaccacccc gccctcctgg
    25681 tctacggaca gcagcatccc cgcgtccggc accaaagcgg tgagttccgc ggccagcgca
    25741 gccgtgtccg cgacgccacc ggactggttg ccgagcagta ccaccgagcc cagttgatag
    25801 ctggtgatcg ccttccgctc ggccgcgtcc agcccgtcgg tcacacccac catgatcagc
    25861 tgcccgatcc gggcgtccag cgacatcgcc tgcgcctcag ccagacagag ttccagttcc
    25921 ggctcgtcgg acggggtagg cggctcgctc tcctccggct ggaccgaagc ggacgccgtc
    25981 ggcgccggag tgggcgtggt cggcgccgac gttggcgtgg tcggcgacgg cggctcggac
    26041 ggcctgtccg gcggcatcac cgagcacccc gccagcagcg cggaccccgc cagcgccgcg
    26101 agtccagcga ccaagcatcc gcgccagccc gtccgccgct tcacccaact tccgtccctt
    26161 tcgggagcga agtttgagtt gatggctggg attggacgca ttacaggtcc agcgccagca
    26221 cggcgttctc gacgacctcg gtcagcgccg ggtggatcca gtactggccg cgggccatgg
    26281 tggcggcggc caggccggtg gccatcgcct ggatcagcgg ctggatcaga ttggacgcgt
    26341 ccgggccgat gatgtgggct cccagcagct gtcccgtccc gcgatctgcg atcaccttgg
    26401 cgaaatggtc ggtgtcctcc atcgcccagc cgtaggcgac cgagccgtag tcgcggacgc
    26461 cgaccacgta gtcgcgtccc tgctggcggc agtcgtcctc ggtgagcccg acggtcgcga
    26521 cctgtggccc gctgaacacc gccatgggga tgaacctgtg gtcactgctg cacaggtcgt
    26581 ccggatgcag gagattgtgc tgcacgaccc gggcctcgtg attggccaca tgcttcagct
    26641 gccatggcga actgacgtca ccgagcgccc agatcccggc ggcggaggtg cgctggtgct
    26701 catcgaccac cacgtagccg tcggcgccga cctcgacacc cgccttttcg aggtcgaggg
    26761 tgtcggaatt cggcactcgc cccgtagcga gcagcagttg ctcggcctcg aagaagtact
    26821 cgatcccgtc atcatcggtg gtgcccacgc ccacaccgag cggggtcgtc tcgatagagg
    26881 ccaggtgctg gccaagccga agcaccaccc gacggctcag cagcttcgtg aaccgcttgg
    26941 acacgtcgtg atcgaacgcg cgcagcaggt ggtcaccgcg gtacagcatg gtcacctggg
    27001 tgccgaacgc cgcgaagatg tgcgcgaact ccgctgcgac cagcccgccg ccgacgatga
    27061 tcagcgattt cggcagtctc tccagtcgca tgattgagtc ggacgtatgc acgcgctcgc
    27121 caagttcagc tgcgtcggcg ccgggcacgt ccggcagccg cggacgggag ccggcagcaa
    27181 gcacgatctg gtcggcgctg atcagctctt cgccgacctg cagcaccttc gccgagacga
    27241 agcgggcgtg ctcacggaag agagtgacgt tagggctgtt gcgccggtac tcctcgccgc
    27301 tggtggcgat cgggtcgatc cggccgaaga tccggtcgcg gatcgccggc cagtcaacac
    27361 tcacctgccc cgtgttcacc ccgaggatgg acgcgcggcg cgccgactcg gccaggtccg
    27421 cggggtgggc gaacatcttg gttgggatgc agccgacgtt gaggcaggtg ccgccgaacc
    27481 acttctggcc atcgatcagc gccactcgcc agtcggcgaa ctgcttggtg atgatcgtgt
    27541 tgccggtgcc cgaaccgatc acgacaaggt cgaagtggcg catgccccga ttgttccatg
    27601 tcgcccggtg aggttcgcct cgacgcgtct cgcagcccct ggcgtccctc gccctctcta
    27661 gtgacaaggt ggaccgcttc tcgccgtcac cccgccaaac cgtccactct gatcagagca
    27721 ccagccacac cgcccacacc accggcaaag tggaccgttc ctcgccgaca cggccccaaa
    27781 ccgtccacca tggcggcgcg gtgacgcctc ccgtccgacg gagactgacc gtgcggggta
    27841 gagccggcgg gtcagatgcc ggcgtaggag tgcaggccgg aagtcagcag gttgatgccg
    27901 acgaagttga accagaagct ggccaggccg atcagcgcca cgatcgctgc ccggcggccc
    27961 ttccagccga ccgtggcgcg ggcgtgcagg taaccggcgt aaatgatcca ggtaaccagc
    28021 gaccaggtct ccttcggatc ccagccccag aaccgtcccc acgcgtactg cgcccagatc
    28081 gcgccggccg cgatggtgaa cgtccacagc gggaacgcga acgcgtggaa gcggtagctg
    28141 atccggtcga gcgcatcagc ggacgggatc cgcgccagat acccgcggac gctccccttc
    28201 gcctccgcgc gggagcgcca caggtagatc agcgcggcga tcccaccgat gttgaaagcg
    28261 gcagccgcgg ccaccgcggc gaagatgtga atccggaacc agactgaatg cagggccggc
    28321 atcaactccg acacgtcgac gtagaagacc gtcaccgcca ggccctggcc gatcgctagc
    28381 agcagggttg ccgggaggcc cagccagcgg gtaccgcgca gcgccatcac caggtagacg
    28441 atcaccaccg cggcgagcgc gctggtgatg aactcgtaca tgttgcccca gggcgcgcgg
    28501 ttggccgcga ccccacggag caccacaccc aagatgtgcg tagccgcggc cacgaacgtg
    28561 atgcccagcg ccgcccgtcc gagcaattcg attcgctggc tgcgggccgg aggtgcgtcc
    28621 tcgaccagga cgccggacgg cgtcacgacg ccgtccggtt cggcggcagc acccaccagc
    28681 accggctcgg gcagcttcac ggcgcgagcg gacgcccact ccgcagcgtg cacggtcatg
    28741 gccagcaggt agagcacggc cgaagtgacc atggccaggc tggagtagta cgcgatcacg
    28801 gctcctcctt gtcggcttca ctattgctca acccacaggc aagcgccaac gctcgtacct
    28861 cggcggcgag accctcgcgt ccgtccaccc ggtcgaggcc ggcgaccgag atggtgagtt
    28921 gtccggtcgg ctccggcact gcccggacga acaagcggcg cggcttcacc gagagggaga
    28981 tcgacatgcc gagcacggcc agcatcaccg agccgagcgt cagccaaccg ccgggtgtgt
    29041 tcgagatctg cagcttggtc cagcgcttcc agccgtcgaa ggagatcgag ccccgaccgt
    29101 cgggcaactc atagctggtg cccacggcga ggccggcgcg caccgggtcg ccgttcggct
    29161 tggtgagctg ggtgagcccg gtcttgttga gtgagtagac gttctcgggc atcccggtct
    29221 cggccttggg cggaccagcc caggcgttca ccagtagttg cgggttgagc acgtccggga
    29281 acaccgagac catgccgttg gcgccgtcct cacccgcggt gggcaggaac accccttcga
    29341 acgccagcct ctccggacgc gcgtccgggg ccttgatcac accgtccgac cggaagttgg
    29401 cgtcctgcgg caggaagacc accggcccgg agaaggccac atcgccattg ccgtcgcgaa
    29461 ccgtcaccac cggtgcgtag ccatgcccga gcaggtggac gctgttccct ccgaccgtta
    29521 ccggagcatt cacctcaagc acctgctggc tgggcgctcc gccagctccg gtaacagtca
    29581 cctcggcccg gaacaaccgc gccgcccctt gctgcaccgg accggtctcg aactcggctt
    29641 cgaacgaatc cagccggacg gtgaagggcg acaactgcgc ggcccggaac ccggcaccgc
    29701 caccgaactc gtcgtactgg gtcagcgtgt tggagaacgc ctggccctcg acgacgatcg
    29761 cggtgccttt gaagctgaac agcgcacccc aggccaggcc gatcagcgtg gtcaccaggc
    29821 agatgtggaa gaccaggttg ccggcttcgc gcaggtagcc ccgctcggcc gagatggcgg
    29881 acgattcccg ccgcacccgg tacttgtgcg cgcgcaggtg cgcctcggcc gcggccagca
    29941 cctcgtcgcc ggatgccacc accagaccgg tggtgaactc gggcagccgg gaaagccgcc
    30001 cgggcgccgg actcggcggc tgccggagcg cgcggacgaa ggtcgcggtg cgcggcagga
    30061 tgcagccgat cagcgagatg aacagcagca ggtagatcgc ggcgaaccaa ggcgaaccga
    30121 acacgtcgta gaacccgagc cggttgagcc actcccccac gcccgggttg gcccgcaggt
    30181 ggtcggccac cccgaccgga tcggtcgggc gttggggcag gaaggagcca ggaatcgccg
    30241 cgatgccaag gatcagcagc aggatcaagg ccgtgcgcat cgaggtgagc gtcgtccagg
    30301 cgaaccgcaa ccagctgaac accgacttca tcagatgacc acctgaaaac cgctgaccca
    30361 ttgccgcatg accgccatca ccgcgtccca ccagccggtg accagcagca gaccgaccgc
    30421 gatcatggcc accccgcccg cgatctggag cccacgatga tgccgcttga cccagtccag
    30481 cgtccgggcc atccgtgcga acgccagccc ggccaccagg aagggcaggc ccagcccgag
    30541 cgcgtaggcc aacgcgagga cgccgccgcg cagtgcggtc gcctcgttca gcgcgagcga
    30601 gagcaccacc gaaagcgccg ggccgatgca cggcgtccag cccaggccga agacaatccc
    30661 cagcaacggg gacgcggcca cgccaaggcg aggcaccgcg ttcagccgca ccgtgttctg
    30721 gccgaagggc agccagccgg tgaagatcag cccgagcgcg atgctgagca ggccgaccgc
    30781 gatcgtgatc ggacgctgcc aggcgatcag cgccgagccc agggttcccg cgagcgcgcc
    30841 ggtggccacg aagacgagcg cgaacccgga gatgaagccg accgtgccca gcaacacccg
    30901 ccgccttgga ccggtgcctt cggtgatctc gaccgcgcct agcccggtcg cgtagctcag
    30961 gtagccgggc agcagcggca gcacgcaggg ggagaagaag gagaccagcc cggcgagcac
    31021 cgccaccggt agcgccagca gcatggagcc gccgatcgcc tcccgagccc aggcgtccag
    31081 atcgagcggc gtcattttcc ggccgccaca tcttcgacca ggcccacgag ggtcgcctgg
    31141 gtggtctcgc cgatgatcct ggcggcgacc cggccgtcgg cgtccaccac cagggtgctg
    31201 ggaatggcgc tggccggaag ctgaccggag aacttcagca gcagggcccc gtccgggtcg
    31261 aacaggttgg cgtacggcac ctcgaaggca cggacgaagg ccttgggcgc tgccatgtcg
    31321 atgtcacggg tgttcaggcc gacgaagatc gcctggccgg cgagctgctt gctggccgcg
    31381 gccagcgccg gcgcttccgc gcggcagggc gagcaccagg atccccagac gttgtagacg
    31441 atgaccttcc cggacacgtc cgcgcttcgc caggtcgctc cgtccagcgt ggtgccctcg
    31501 atcgcagggg cttcgggacg atcggcgatc gccagcacgg tgatgctgcc gtccccgccg
    31561 acgaaaccgc cggagctgga cggcgctgaa ctgcagccga cgaacagcgt ggccgccgcc
    31621 attgcgacca gcaaccggta ggcgcgcatc aggcgccagc cctaccgtgt ccccggcaaa
    31681 tccaaccgtc gtcccggcgt tggcccggat ctccgcaccg gtccacttcg atcaggctcc
    31741 cggacggaac ttcttggcct tgggcggcac caggtcggcg cagggctcgg cgtaatcgac
    31801 ccggaccagg tgcccgtcgt ggtaggtgaa ggacgtgacg ctggccaggc tggtctggcg
    31861 acgccgcggg tcgtgcacga gccggcggcc ctcggcccag gaacgagcca tccagatcgg
    31921 cagttcgtga gccaccacga ccgcttcacc gccgaccgag ttcgctgcgg cgtcggcaag
    31981 cgctgcctgc atccgggcga cgatcgagac gtaggcctcg ccccacgacg gacgcagcgg
    32041 attgcgcatc gcccaccaac tcgacggccg gaacaggatc tcgttgcgct tgccgaagga
    32101 cttgccttcg aagacgttgg cggcctcgat cacccgctcg tcgagatgca cctcgagttc
    32161 ggggtgcttc gccgcgatcg gagccatggt ctccagcgca cgctgcagcg gggagctgac
    32221 caggtggacg aggttcgcgt ccgcgaaata ctcaccgagg cgttcggcca tccgctggcc
    32281 gagttcggag agcccgaacc ccggctggcg gccgtagagc accccttcgg ggttctccac
    32341 tttgccgtgt cgcaccaggt ggacgacgct gatctccatt ggactcctcg ctagctgtgg
    32401 ccccaaggat acgcagccgg acttccacgt cgtgagccga gcgccgcggc caacgcggcg
    32461 gcgtccacat gccaaatggc gtggcgcgcc ccgtccacga aagtgaccgg aacgtggtcg
    32521 ccgaactcgg cgcgcagttc caggtcactg tcgacgtcaa cggtcgtcca ggtcgcatcg
    32581 gcggatgcgc agaccgcgcg cactatcgcg accgcctcgt cacagaggtg acagccgtca
    32641 cggaccagaa gtaccacccg ggcttgatcc atcagtcagc ctgggaagcg atggcgacta
    32701 cttgccagcg cgacgacgct ggatgcgcgt cttcttcaga agcttgcggt gcttcttctt
    32761 cgccatccgc ttgcggcgct tcttgatgac cgaacccact ggcagacctc tcgaatacca
    32821 ccccgggagg ggcggcaagt aactggctcg ggctggggat cagcccgaaa ggacatggcc
    32881 ggacattcca gccagacaca ggatcttact tgaacgcgag ccgctggagc gaatcagccg
    32941 gctcggtaga acgacccacg caggtaggcg tcgaccgcat gctcgggcac ccggaagttg
    33001 cggccgaagc ggatcgcctc cagatcgccg gaatggatca ggcgatacac cgacatccgc
    33061 gacacccgca gtgctgcggc gacctcggcc accttcagaa acttgaccgt tcccaacggc
    33121 aagaccgggt cgttcatcgg cgaacctgtt tcactctggg ccgtccggac gagggccgct
    33181 cgacccaccc tagtgctcga ccagcgcccg cgggcccgga tgacgaactc tgttgagctc
    33241 ggtggcagtc agcccgctgc catagcgtgg cgccaggcga acggcccgag cctgcggacg
    33301 cgaatgctgg cgacctgcac cgcgaactcc agggccgcca gtcgcgacca gccggacgcg
    33361 agcccgaacg cgtaggcgcc gtgcagcaca tcgccggcgc tgagggtgtc aaccaccgat
    33421 gcggacggcg gctggaccac gccatgctcg ccgtccgacc accaaacaag cgcatcggca
    33481 ccatgactca ccgccaacaa ccgccgccct gcagccagtt cagccggagt gagacccgcg
    33541 gggtggacgt agtcggcgga gcagatcacg tcggtgctca aggtcagcaa ctggtcgaag
    33601 accggcttgt gggaacccgc atccacgact accggcaacc cggacgccga cgcctcacgc
    33661 gcggccgcgg cagccaattc ggcgtggtgc ccatcgatca ggacaacatc agccgcggtc
    33721 aggtccggct tcggcggcga cgccaggtgt ggccggtgcc cggagccact gatcaccgcc
    33781 cgctcgccgg tggccgcgtc cagcagcaca ctggaggtcg ccacctcgaa cccgtccgga
    33841 gcggcatcga tcacttccac cccagcggac gcgaggtcgg cgcgaatcgc gtccgcggcg
    33901 gcgcctccgc ccaaggcggt ccacagaacc gcccggccgc cgagcgcggc gaaggcgatc
    33961 gcggcgaccg tggccggccc gccggcagcc agccaggtct gcttcgcggt ggtcttctgg
    34021 ttgggtgccg gcagcacgtc caccagctgg atcacgtcaa gcgtcgccag gccgacgaac
    34081 cagccgaccg gacccggcgc cgcccccttg gcttcgctca ggcccggcat gcgattccgc
    34141 cgcgctgctg gtcgagcacg cacagcggca ccccgccggg cgtctccagc atcgtctggg
    34201 tctcccgcac cgagcgagtt atcgagccgg cagcggtcag ccgagccacc tcagccgacc
    34261 ggtcgtcggt cacctggtgc aggtggccca ccacttccct cgcgatctcg gccggctgca
    34321 agcgcaggtc gagcgccagc tcaccagcct tgcgggtgat cgcttcaccg gcatcgggcg
    34381 gcgtccactc gccggcaagg atcgcttgcc agaacgccgc ctcagcactg aagcgcgccc
    34441 tggggacgtc ccagcacacc tgcgccagcc ggctcgtctg cccggcaggc cacaccgcgg
    34501 ccggcggcac ggtggcggcc ggctgggtca cgaagcagag cacgattccg cccggagaac
    34561 gcaagaccac gtagctgtgg cgcgagtcgt caaccaactc cgcccccaga tcctcggccg
    34621 cttccgccgc agcccaggga tcgaccacat gcaggtcgag atgcagccgc gtgaccccgt
    34681 ccccgagccg ctgcacccgt aggtagtcgt cgccggacgc gggcagcagg gtcgcgaact
    34741 cggagttctc gccgcgcggc gccgacgcgt caaagccggt ggcgtgcgtc cagaatcgga
    34801 cgcccgcatc gtactcagcg gccggaaagt cgaggaacgc ggtcaaccag aacggtcggg
    34861 cggcccccat gtcaggacga ggccagcttc cggctgcggt cgcgggccgc ctccatggca
    34921 gtcatgaacg ccgccttcac ccggtggtcc tccagctccc gcaacgcggc cgcagtggtg
    34981 ccacccggtg acgtgacccg ttcccgcagc acggtcgggt gcacacccga ctccgaaagc
    35041 agcttggccg agccgtacat ggtctgcacc accagggtgg tggcgatgtc ccgcggcagg
    35101 cccagcatca caccggcgtc gatcatggcc tcgacgacga agaacaggta ggccgggccc
    35161 gatccggaga tggccgtgac cgcgtcctgg tacttctccg gaaccgtgac caccttgccg
    35221 accgccgaca gcagcccggt gacgtgttcc aggtgctcgg cggtcgcggc ggaaccggcc
    35281 gagatcgcgg ccatgccctc gtccacctgg gcgggcgtgt tcggcatgac ccggacgacc
    35341 ggcgtgcctt cgggcatggc ggcttcgatc cgcgaggtca ccagtccggc gcacagcgag
    35401 acaaccagcg tgccgggacg gatcgcgcca cccacctcgg gcagcacctc gccggcgtcc
    35461 tgcggcttca ccacgaccac caccgtgtcg gcctccgcag acgcctcggc agtggagccg
    35521 gtgcggatcc cgtagccagc ttccatctcg gccagccgtg cgttgtggcg gtccgccgcc
    35581 gtgatctcgt cggcggccca gccggcccgc agcagaccgg cgatcagggt ctcccccatc
    35641 acgccggcgc cgatcacggc aagcttggtc atcgcttcgc caccagctcg gcgatctgga
    35701 cggcgttcag cgccgcaccc ttgcggagat tgtcgttgct gacgaagagc accagcccgt
    35761 gaccgtccgc gacggactgg tcggcgcgga tccgtccgac gtagctcgga tcggtgccgg
    35821 ccgcctcccg ggggttgggg atgtcggtca gcaccacccc gggcgcggtc gccagcagcc
    35881 cggtggcccg ggtgacactg atctcctctt cgaactcggc gtggatgctc agcgagtgcc
    35941 cggtgaacac cggcacccgg acgcaggtgc cggcaaccag caggtcgggg atgtgcagga
    36001 tcttgcgcga ctcgtgccgc agcttctgct cctcatcggt ctcgccgctg ccatcgtcta
    36061 cgagcgcacc ggccagcgcg accacgttga acgcgatcgg cttcaggtag ggcgccgggt
    36121 cgccgaacgg gacggcacgg ccatccagcg ccagccgagc cgggtcggcc gcggctgcgg
    36181 tctggtcggc cagcgcctgg acgcccttca cgcccgagcc ggagaccgcc tggtaggtgg
    36241 ccacggtgag ccggcgcagc ccggccgcgt catgcagcgg cttgagcacc ggcatcgcgg
    36301 ccatggtggt gcagttgggg ttggcgatga tgcccttcgg cggatcgatc gcgtcctccg
    36361 ggttcacttc ggacaccacc agcggcacgt ccgggtcctt gcgccaggcg gacgagttgt
    36421 ccaccacgat ggcgccggcg gcggccacct tgggcgcgaa ctcgcgcgac atcgtcgccc
    36481 cggcggagaa gagggccaga tcgagaccgg agaagtccgc ggtcgcggtg tcctccacca
    36541 ggatctcgcg atcccgccac gacagcggct tacccgccga ccgcgccgag gagaagaacc
    36601 gcagttcgtc caccgggaaa tcccgctcgg caagcagcgt ccgcattact ccgccgacct
    36661 gaccagttgc accgaataca ccaacacgca taggggccag cctaatgcca gccacccaca
    36721 gcggtccggg tgaggcgtcc gccgtggacg cagaccgcgc cggaggcgtg cggctcagcg
    36781 catgatctcg cggttgaacc ccaggtagcg ggtgcccttc atggtgaccg tgccccggtc
    36841 gccgacgacc agcaatccgt actcggacgc ggatacctgc aactcgaagc gttcgccgct
    36901 gggctgttgg aaggtcacga agtgggtctg gctcaccgac gactcgccgc cgccgtgggt
    36961 ctcgacgcgc ttgtccacga tcatcgccac ggcgttgacc tcgggggcag cctgattggc
    37021 cgcggcggta cgtccggctt tgaccagacc aaagatgatc gccccgatga ccaccacaaa
    37081 gacgatgccg atgaatacgg ccacgaccgc gaacatgatc ccgaagccag tcatgccagc
    37141 accctaccga cgaaatccgg acgccacgga ctgctcacag ccacgacacg tacggcgcga
    37201 tcagcgaata gcccacgaag gcgaagaaat cgagcaggaa gtgcgcgacc accagcggac
    37261 ccacgcgttt gaatcgccaa taggccaggc cgaagacgat gcccatcacg atgttcccga
    37321 caaagccgcc atagccctgg taaaggtggt agctgccgcg caccacggcg ctggagagca
    37381 gcaccaccca cggtgcccac ttcaactgtg tggcgcggac gaaccagaaa ccgatcatca
    37441 ccacctcttc gaggatggcg ttcatcgctg cgagcgcgat cagcaccggc accgtccacc
    37501 agttcgccgc gagattggcc ggtgccacgt tggtgttcag cccgaacgca cgcgccacga
    37561 agtacagggc cagcccgggg ataccgatca ccgcggccag tccgaagccg cggctgaggt
    37621 cgaacccggg acgccgcagg tcgaagccga tccgcgtccg gtcggcgctc cgttgcagca
    37681 ggtagagcgc caacagtgcc gggaccaggg gaaataccac tccggccact tggtaggaga
    37741 ggtcgagcca tgggcggtcc ggcacgaccg aggagttgat cgacgtggtc tgctggttca
    37801 gcggagtgcc ccgggtggcc ttttcgagga tcgagaggat cgagtagacc gcggactggc
    37861 ccaacgacac ccccagcacc aacagcagtt cgatgcccgg aagcgtccga ccgtccgccg
    37921 ggagatcgct ggtcagcggt tcgggattga gttcggcggc ggccatcaac ccgcccgtcc
    37981 agcaccgacg gccaggtgct cgcgagcgaa cgtcaggctg tcggcgatca gtttcactcg
    38041 atcggcacgt tccatccccc gagtctgcac ctcgagtacg acatggcccc ggtaaccctg
    38101 ggcggccagc gtggcgagca ccgtggccgc gtcctggtcg ccggcgcccg gggccaggtg
    38161 ttcgtcggtc gcgccgtaac ttccgtcggt gaggtgcagg tgtgccagtc gctcacccca
    38221 gtcgtggacc agcgcgcagg acgactgccg cgctgtcgct gcgtgcgaga ggtcgagggt
    38281 gagatggtcg cagtcaagca gggacgcgtc ccagctgggc gcgtaggcgc gcacctcgcc
    38341 accgcctgga ccgcgccacg gatacatgtt ctccacggcg aaggtgagcc cggtcgtcgt
    38401 ctcaagccgt cggatgccct cggtgaaccc ggacgcatag ctgcgctgcc agcggaacgg
    38461 cggatgcacg accaccacgt ccgcaccgag ctgatgggcg gccatcgccg atcgctccag
    38521 cttctcccac gggtcggggc cccagacccg ctgggtgagc agcagcgttg gcgcgtgcac
    38581 cgacacgacc ggaacttcgt ggtattcggc cagccgttgc accgcgtcca cgtctgctga
    38641 agtggggtcg gcgccaacca tcagttcgat gccgtcgaag ccgagttgcc gggcgagcgc
    38701 gaagccggct gcggtggatt cgggatagac cgaagacgtg gacagggcca gcttgggggc
    38761 tgacgcgttc acggatggca cgacgcaatc gtaggcgctg ggtccgacac ctctccgagt
    38821 gctgtgcagc aaactccggg cgaactccgc gagccggcca aacctgcaca acggtttggt
    38881 ttccggcggt gacggaccgc cggacccggc acggtcaccc tcgattcgcg tcaaaggtgg
    38941 cagatcgcca caatgggttc atgcatgtcg atcatctgac cttcgcggtt gggtcggcgg
    39001 gactgaaggg tgagtccaag cgactcggcg ccctgctcgg cgagcggttc cgggacggcg
    39061 gattccaccc gcgattcggc acccagaacc acattctccc gctggccgat gaccgctacc
    39121 tcgaagtcgt cgaggtgctg gagcacccgg ccgcggagaa ggctccgttc gggcaggcgg
    39181 tccgttcccg ctcagagatg ggcggcggtt ggctcggttg ggtagtttcg gtcgatgacc
    39241 ttgccccctt cgaggatcgg ctggatcgcc aggctgtgca cggttcccgg catttcccgg
    39301 acgggcggct gctcgagtgg gaccagatcg gcgtcaaggg cctgatcgcc gacccgcaac
    39361 tgccgtactt cttgaagtgg gactccgacc cgtcggtgct gcccagcgcg ctggcgggca
    39421 cgatccggct ggagaagatc gagatcgcgg gcgatcgctc ccgggttgag gactggctcg
    39481 gcatccagat cggcgacgag ttcgacggcg tccggatcaa gttcacccgt cccgccggcc
    39541 agcccggcat ccagtcggtg accttcgaca ccccgcacgg cacggtgcgg atctagggac
    39601 gcgccttagc gctcccccac cccccgcgcc gcgggcaccc acgcgccccg cgcgtccgcc
    39661 attttggcaa cttcgctccc gaacgggact cccgctcccg aaagttcggg agcaagggtc
    39721 ccgttcggga gcgaagttga gttatgggtt gatgctcctc agccgcgctg ctggctcgga
    39781 agtaacgtca gaagcacccg ctagcgtctc tggcatggcg aagagcacga gcaattccta
    39841 ccgctgcacc gagtgcgggt ggacgactcc gcgctgggtc ggccgctgcg gcgagtgcca
    39901 ggcatggggc agcgtcaccg aggggtcggc gccttcggcg agggtcgcct cgtcggtgcc
    39961 ggcgagccct gcgctaccaa tcggcgaggt gccgctggag gccgcgcgag ccatccctac
    40021 ccggctcaag gagctcgacc gcgtcctcgg cgccggcctg acgccgggcg cggtggtact
    40081 gctggccggc gaaccgggtg taggcaagtc gaccctgctg ctagaggtgg cgagtcgctg
    40141 ggccagagcc ggacgccgca cgctgtacgt gaccgctgag gaatctgcgg cacaggtgcg
    40201 gttgcgggcc gagcgcacct cggcggtgat cgacgggctc tatctcgcgg cggagaacga
    40261 cctcggcaac atccttggcc acatcgaaca gctctccccc gagctattgg tgctcgactc
    40321 cgtgcagacc gtacaggtgc ccggcgccga ctccgtcgcc ggcggcgtcg cccaggtgaa
    40381 ggaggtcact gccgcgctcg tgcggatcgc gaagcaacgc gcgctgccgg tactgctggt
    40441 cggccacgtc accaaggacg gcaatgtcgc cgggccccgc accctggaac acctggtgga
    40501 cgtcgtactg tccttcgagg gcgatcgcat gggcggcgtc cgcatgctgc gggcgatcaa
    40561 gaaccgctac gggccggccg acgaggtggg ctgcttcgag atggtcgagt ccggcatccg
    40621 cgaggtcacc gatccgactg ggctgttcac ctcgcagcac accgagccgg cccccggcac
    40681 ctgtctcagc atcaccatgg ccggccggcg tccactgcta accgaggtgc aggcgttggt
    40741 ggcacccaac tccggtccgg tgccaagacg ggtgacccac ggcatcgaag gcgggcgggt
    40801 ggcgatgatc ctggcggtgc tgcagcgaag ggccggcgtc cgcctgcaca cccgcgatgt
    40861 ctacgtctcg acggtcggtg gagcgaaagt gtccgacccg gccaccgacc tggccaccgc
    40921 gctggccata gcgtccgccg cctgcgacga gccgctctgg cagccggtga tcgccctcgg
    40981 cgaggtgggc ctgtcgggcg agctgcggcg gctccccggg gtcgaccgac ggctcgccga
    41041 agcggcccga ctgggcttca agctggccct ggtgccgtcc ggtagcggcg cccgcaccca
    41101 gggactgaag gtggtcgagg tgggcaacgt cagggacgcg ttggcggcga tgcaactgca
    41161 tcctcgaagg aagcgggaag agcaaagacc gcacctggag cttgcttgac ggccgtttcg
    41221 tcctatgcca gatgagcctg ggtgggccgg gaagagacgt tccccgtcct cctgcccagg
    41281 ccgccgggcg taccggatgg ccaagcccgt gccggccgtg gatcagttca ggccttgcgc
    41341 accacgaaca cctgcttggc gctgagcgcg tccgcgagag ttgccgtgcc gacgtaggtg
    41401 ccaggtttga tcaggtcctt ggtgttgcgg cagccggacg ccgaccgctt cagcggccac
    41461 tcgacggtga actcgtaggc cttcttcgac ttcagcgtgg tcttcttcgt cgggccccac
    41521 ttggcacaat cctgggtggt ccagattttg tcggtgccgc tggtgatcgc gaacgtgaac
    41581 gtctcgggcg agaggtcgag gacgcactcc gccgagccct tgttcgtgat cgccaccttg
    41641 aacagttgct taccgtcctg cttaaccttc tggtaaccgg ccaccgacaa gtcgctgttg
    41701 gtgccgtcgc aggtcagcgg gccggtgggc gtcggggcgg ccgaaggcgt ttcggatgcg
    41761 ctcggtgagg ccaccgcggt cggcgtggtg gccgcggacg gggttgccgg gacggccgcg
    41821 acgggcgcat tcggagcggg ctggaacatc gagagcacca gccacagcac gaggagggcg
    41881 gccgcgacga gggcggcccg ccgcgcccag tagacccaga caggttgttt gccaaccggg
    41941 tgcattaccg aacccatgcc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    42001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    42061 cccagtagac ccagacaggt tgtttgccaa ccgggtgcat taccgaaccc atgcccacag
    42121 gctaggtggc cgcgtcgccg ggccgcgcgc accacgccgt ccgcgcggca caaccgcctc
    42181 gggtacggtt ggactgaaac cagcagtgcg gaagccctgc cagctaccga cgaccgtttg
    42241 acgtccagcc cgaggtggag atgaccaatc cgtacgcccg cccctacgag cgtcctgagg
    42301 acgccgatct gaccattccg ctggttcctg attccggcta cagccagccc agccagccgc
    42361 cgttcactga agagacgctg gtcgatccgc taccgcaggc accccgcgac ccctggggcg
    42421 tcgcctcggc gcaggagttc ggctacaacc ccactcccgg tcaggcgccg accggttcga
    42481 gcccccagcc cgcccagacg cagcccaact acacccagcc gcaggtgggt cagcagtcga
    42541 gctatcagcc gtacccggcg tccgactacc cgccgatggc cgcctacccc acgacggcgc
    42601 agcccacgca accgtccgcg gtagcgccgt ggtcggcctc cgcccccgtg ccgtacgcgt
    42661 tctccgaggc ggccctgccc gagcacccga gcgcgaccac cgcgctggtg ctcggcatcc
    42721 tgggcatcgt ggggctctgg ccgctcgggc cgatcgcgtg gatcgtgggg gcgaaggcca
    42781 agcgcgagat gcgagccgaa cctggccggt ggcgctccag cggcagcctc acgaccggcg
    42841 tggtgctcgg cgtgatcacg accgtgctga tggtgctcat gaccctggcg atcctgttct
    42901 tcgtcttcct ggtcgccgtt gcctcgaggt aagggctcgc gaacaccggc gagtttgccg
    42961 gatccgacgg ttcggtcaga cagaagtggt tcactcgacc catgagcgaa cgatctgatc
    43021 ttgaattcct tcgcatgctg caccggctgg cccgctcggc cgagtcggcg gagacgtcgc
    43081 gtccaggcaa cctcacccgg ctgggcgccc tggttgccgc ccacctcggg gtgccggccg
    43141 aagcgaacca gttgctcagc ctgccgctgc ctgcccaccg cctggtggac gctgacctgg
    43201 cgctcagcca cttggccgac gccgacccgg acacgacggt cgtcgggatc agcggtttcg
    43261 cccgccacga ggacagcctt gccgccctgc tggtgaacga gcacgccgac gtgacccccg
    43321 gcccggtgga ctacacctgg ctttccaccg ggcccgacac ccgtcgccag gtggtgagca
    43381 acggcgtccg gctgttccgg ctcggtgacc gcccggtggc ggtctggcag cgcgcaagcg
    43441 atccgcgaag cggggaggag cgggcgacgg tacaggtggc ggcggcgacg gcagcggact
    43501 cagagcaggt gatcgcacag gtacagaacc tgatggccga actaagcgtg ctgcgcggcc
    43561 aagtggtgtc gttcaccggt aatgagttcc agccgggaat ggccggcatc agcttccacc
    43621 accgaccgca ggtggaagcg ggtgccatcg tgctgcctga gggagtgctt gatcgggtgg
    43681 tgcgtcacgc ggtcggcatt ggccagcagg ctgagacgct gcgccggcac ggccagcacc
    43741 tcaagcgtgg cctgctactt tacgggccgc ctggcaccgg caagacactg accgtacgcc
    43801 acctcatcgg cgaggcgagg cagaccacct gcatcctgct gagcgggaac agccttggta
    43861 tggttaccgc ggccgccgaa ctggcgcgcg ccatgcagcc tgcgctggtg gtgctggagg
    43921 acgtagacct ggtcgccgcc gaccgggagt tctacggcgg tccgcagccg ttgctgttcg
    43981 cgatcctcga tgccctggac gggctggacg gcgacgcgga cgtggctttc atcctcacca
    44041 ccaatcgcgc cgacctgctc gagcccgcgc tcgcacagcg ccctgggcgc gtcgacctcg
    44101 cggtggagat cccgttgccg gacgcggccg cgcgccgccg gcttttcgag ctttacgccg
    44161 ctgggctgcc gttcgacccg gccgcggtgc acacggtcgc ggaggcggcg gcaggggtga
    44221 ccgcctcatt tgcgaaggag gcggtacgac gagcggtgct cgaagcggcc ctggccgacc
    44281 gcgaaccgac cgacgccgac ctgtcggccg cggcggctga gatgctcgcc ggcggcgccg
    44341 ggctcaccag agccctgctc ggctccccgg acgcccggca cgagcactac ggcctgatcg
    44401 ggttcgccga ccacccggtc ggtttgtccg actgacccgg accgccaggt cggtcaggac
    44461 ggctggccga actccgccgc cagcgaatcc ggcaccagcg a
//
LOCUS       DAOPRQ010000008        42488 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_690_length_42488_cov_12.409540, whole genome shotgun sequence.
ACCESSION   DAOPRQ010000008 DAOPRQ010000000
VERSION     DAOPRQ010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 42488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 42488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42488
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_690_length_42488_cov_12.409540"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1508
                     /locus_tag="PKJ61_01705"
     CDS             <1..1508
                     /locus_tag="PKJ61_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004808575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HOC12582.1"
                     /translation="VQRTFYARGQTGQQLLIGAYQALERQVAAQTVKMYTRHEMLDLI
                     VVDGRARGIVTRDMVTGAIESWTADAVVLATGGYGNVFFLSTNAMGCNVTANWRAHRK
                     GAYFGNPCYTQIHPTCIPVHGETQSKLTLMSESLRNDGRIWVPKKAEDCGKDPRQIPE
                     EDRDYYLERIYPAFGNLVPRDIASRQAKNMCDEGRGVGPAVKEFDHDGNEVFTRRGVY
                     LDFADAIGRLGKDGVAARYGNLFDMYAQITGEDPYEVPMRIYPAVHYTMGGLWVDYDL
                     MSSIPGMYVTGEANFSDHGANRLGASALMQGLADGYFVLPNTINDYLAGAASFGKVDD
                     SHPAVVAAVAETTERIERLLAVKGTRTVDSFHKELGHIMWEYCGMERSEEGLKLAITK
                     IDELLEEFWRDVKVTGVNEDFNQTLERAGRLLDFFELGRLMCVDALHRRESCGGHFRA
                     ESQTEDGEALRHDDEFLYVAAWEYAGTGAKPVLHKEPLVYEAIELKQRSYK"
     gene            1505..2266
                     /locus_tag="PKJ61_01710"
     CDS             1505..2266
                     /locus_tag="PKJ61_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HOC12583.1"
                     /translation="MNITLRVWRQANAQTEGRMVTYQVQNVSSDMSFLEMLDLLNEDL
                     TIKGEEPVAFDHDCREGICGMCGVVINGTAHGRGDSPVPTTTCQLHMRSFSDGATIDV
                     EPWRAGPFPVIKDLVVDRSAFDRIIQAGGFISANTGAAPDAHAAPVGKLKADRAFDAA
                     TCIGCGACVAACPNSSGMLFTAAKITHLAMLPQGQPERYSRIKSMIATHDAEGFGGCT
                     NIGECAEVCPKQIPFDTISQLNRDLIASLFKKDGK"
     gene            2475..2825
                     /gene="rplS"
                     /locus_tag="PKJ61_01715"
     CDS             2475..2825
                     /gene="rplS"
                     /locus_tag="PKJ61_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015070816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HOC12584.1"
                     /translation="MNQLVEQVGKAALRDDVPDFRPGDSVKVHVRVIEGNRSRIQVFA
                     GAVISRTGSGIAESFTVRKVSFGTGVERTFPLHSPIIEKIEVERRGDVRRAKLYYLRG
                     LRGKAAKIKEKRDR"
     gene            2882..3652
                     /gene="lepB"
                     /locus_tag="PKJ61_01720"
     CDS             2882..3652
                     /gene="lepB"
                     /locus_tag="PKJ61_01720"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOC12585.1"
                     /translation="MSAPTSAGNGRKAWSVIGSWLREIAIVVVGALVASTLLRLFVVQ
                     MFVIPSGSMENTLLEHDRVAVQKIVGFERGDIVVFRDTQNWLIPVPVKDDPVVKTLVF
                     LGLAPDESTGHLIKRVIGVAGDHVACCDARGRVTVNGVALNETEYLYTDPATGKQVDP
                     SSVAFDVVVPAGRVFMMGDHRNDSQDSRCHLDEQLDSDPAGSAAFIPVENIVGTGVAV
                     VYPFARMHTISRPLTFESIPAGQDPPTEAVITGPKVLC"
     gene            3660..4391
                     /locus_tag="PKJ61_01725"
     CDS             3660..4391
                     /locus_tag="PKJ61_01725"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004808567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOC12586.1"
                     /translation="MAVVVRRDAGIYAYEATLSRAGFGLIAGADEAGRGACAGPLVAG
                     AVILRLGAAGEIPGLADSKLLSAAQRERVYAEVTARALSWSVVCVEADECDLLGMHHA
                     NLTALRRALLRLEPTPAFALTDGFGVDGLGVPNLAIWKGDRVAACIAAASVVAKVTRD
                     RIMTQWHESLPHYAFDVHKGYCTDLHQERLDQHGPSPIHRWCFENVRRTASASACETS
                     GDGSAPDAAVVREATTTAAPLLDIE"
     gene            4399..4710
                     /locus_tag="PKJ61_01730"
     CDS             4399..4710
                     /locus_tag="PKJ61_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016455603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2469 domain-containing protein"
                     /protein_id="HOC12587.1"
                     /translation="MSTEDLEQYESELELQLYKEYKDVVGIFTYAVETERRFYLCNAV
                     DLKVRTEGGDVYYEVSMGDAWVWDMYRPARFVKSAKVLTFRDVSIEEIAHSELKVPDN
                     L"
     gene            4957..5322
                     /locus_tag="PKJ61_01735"
     CDS             4957..5322
                     /locus_tag="PKJ61_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOC12588.1"
                     /translation="MGRAEVWHAGEDLAAAHLQRLGWQVLDRNWTCPVGELDIVAIEP
                     GPEAVVVFCEVKTRRGLGFGTPLEAITPAKVAKLREVALHWLRARPVPVPHIRFDGLG
                     VLLPRDAQPVITHCRGIGS"
     gene            5319..6842
                     /locus_tag="PKJ61_01740"
     CDS             5319..6842
                     /locus_tag="PKJ61_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016665659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HOC12589.1"
                     /translation="MRQARAWSVALVGIEGRMVEVEAAISSGLPRTTMVGLPDAALYE
                     ARDRCRAAMAAVGPGWPSDPVTINLSPATLPKAGSHYDLAIAAAVAAAGRRFHPDALE
                     GLVLFGELGLDGRIRRVRGLLPALLAAIGQGFGRAVVPATQLREAALVDGLVAYGANR
                     LDDLFDILEGGPGADITPAEPTVDPVPTGRDLADVAGQVEGKWALEVAAAGRHHLYFH
                     GPPGVGKTLLAERLPTILPDLELWEALEVSAVHSLAGLALDEGLVRRPPYSDPHHSAS
                     MASLVGGGARIAMPGAVSRAHRGVLFLDEAPEFSPRVMEALRVPLESGRIVLGRAAAT
                     ATYPASFQLVLAANPCPCGMAGTPGGDCRCAPMAVRRYAARVSGPILDRIDIHQQLRP
                     ARASFLGNQVVGENSTTIAARVAAARERQLHRLAASGWRSNGEVPGPALRKRLPLPDG
                     AELLDTAVARGRLSARGVDKVLRLAWTVADLQGSAKPSREHLHTALAMRRGETLEAK"
     gene            6839..7963
                     /gene="dprA"
                     /locus_tag="PKJ61_01745"
     CDS             6839..7963
                     /gene="dprA"
                     /locus_tag="PKJ61_01745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOC12590.1"
                     /translation="MTDERTARMCLAAVVEPGHPTIAEAVLSYGAAAVWASLTRPGAD
                     LPLSRRARVLVVEDLLTATRALGMRFVVPGDAEWPGGTEDLRSCEPVNQLTGEPLGLW
                     LRGAGDLAELAGTSVAIVGSRASTPYGDTVAADLGAELSEAGRSVISGGAYGVDAAAH
                     RGCLAGRSPTVAVLAGGLDQAYPAGHHQLFERIAERGVLVSELAPGEHPTRVRFLARN
                     RLIAAMTPGTVMVEAAARSGARNTVTWANELGRVVMAVPGPVTSATSVTPHRLIREAE
                     AVLITRAPDVLELLSPLGRPNPRIPGGRRETDDLSAEELRLFECVPGRGSLPAAELAL
                     RADLSLPRCLALLEELADRGFVAQNLTGEWQLPPRARKTG"
     gene            8150..8872
                     /locus_tag="PKJ61_01750"
     CDS             8150..8872
                     /locus_tag="PKJ61_01750"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HOC12591.1"
                     /translation="MPTGTDSTLTPQVARSAEVSLRVTDVMVAAQRLRELASAAGGQV
                     TAESLVLDAKDQSYSAVVISVDADKLDSTLTALAEVGTVESRVINSEDVTTQVADVNA
                     RVAALNASIQRLQELSKKAGSVEELVRVETELSNRIAERDSMVAQQKWLSGRVAQSQI
                     SVQLITPEQAGPLQTTGFMGGLLAGWNALVATGLTLLTVVGALLPFAAVALLIALPLL
                     FWRRRVRARKRQSVPDPPPTVD"
     gene            8973..10205
                     /locus_tag="PKJ61_01755"
     CDS             8973..10205
                     /locus_tag="PKJ61_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HOC12592.1"
                     /translation="MHKLTVAQGTALSAGAVLGTGIISMPALAAAVAGPASLIAWLAL
                     VLLSAPLAWTFAELGARFPDGGGVSSYVRRAFGTWAADAIGWIFYFAVPIGAPVACSF
                     AAGYVADVLGGGQVARIGTFLGIIGIVFLMNWLGLHVSGRMQLVLAGVLALVLLTTVL
                     AALPHADPGNLTPFAPAGLGAIGSAAALLVWAFAGWEAVSSLSPEYADPRRDVPRATM
                     LAVVVVGVLYLSVAVTSVLVLGPQLAASQAPLADLLATGVGGPVRPIIAVVAVLMTLG
                     AVNAYMAGATKLGSALAAEGALPYRIFGTDRKALMFVVGAAVVSEWLLADQHTLMLLV
                     SGGLTLVYVVGTAAAVKLLPGPGAKAVAAFSLACVSVLLVLTGWPALAGLGIAIAAVL
                     HRLWADRRRRLAKSSKFG"
     gene            10217..11338
                     /locus_tag="PKJ61_01760"
     CDS             10217..11338
                     /locus_tag="PKJ61_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011758045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOC12593.1"
                     /translation="MALSPREREILALLRAHPLLDASGLAQRLGTTKGAIAVALSSLT
                     QKGEIVGRGYLLRSDAWAVVVGGAAWDIKARSSAPVRVGTSNPGTVSQTPGGVGRNIA
                     EGIARLGGQVHLIAAVGSDGSGRDLLTRTAEAGVHVDRMIDSTHPTASYLAALDADGE
                     LVVGLSDFAATDALTVADLARSQDLIANAAVLVVDGNIPAPVVDWLLAVAAGSGVRVV
                     LEPVSVAKAGRIAGLLSPDRPVFAITPNVDELGALVGRPVADTEVAIAEAAADLHSRG
                     VSHVWVTRGASGSLLVSADAPAPQRLDAPASEVVDVTGAGDAMTAGFVHGLLIGESPL
                     SSAQLGQLAAALTVASRFTVRPDLGVALADLHRTLEGVR"
     gene            11335..12243
                     /locus_tag="PKJ61_01765"
     CDS             11335..12243
                     /locus_tag="PKJ61_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007079508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HOC12594.1"
                     /translation="MNPLLALTDEVREALASGKPVVALESTIISHGMPYPKNVQTARE
                     VEQIIRDGGAVPATIAILHGVPRIGLSDAELDLLGSDENVRKVSIRDLPYVVARKEHG
                     ATTVASTMRLAALAGIKVFVTGGLGGVHRGAESSMDVSADLTELSLTDVAVVSAGVKS
                     LLDIGRTLEVLETLGVPVISVGSDEFPSFYSRSSGFRSPLRLDAAAEIAAAMHAKWDL
                     GLAGGLVVANPIPIEDEIPEAEIGALIARAVAEADEQGITGAAITPFLLGRIVELSDG
                     ESLRANIALVKHNAQLGAAIAVAYAG"
     gene            12657..13178
                     /locus_tag="PKJ61_01770"
     CDS             12657..13178
                     /locus_tag="PKJ61_01770"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: protein motif:HMM:NF014753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HOC12595.1"
                     /translation="MRSGVKSEQGATLAEVRAIGEKVNSVTRTMGIALTACTPPAKGS
                     PLFDLGDDEMAVGVGIHGEPGRRRAKLVSAEEIVDELLGAVVTDRPHASGDRVALMIN
                     GLGGTPISELYLLFGIAAQKLADQGITVARSYVGEYCTSLEMAGASLTLVKLDDEIAT
                     LLEAPASIVNRIF"
     gene            13320..14795
                     /locus_tag="PKJ61_01775"
     CDS             13320..14795
                     /locus_tag="PKJ61_01775"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOC12596.1"
                     /translation="MPPDKPQHRILPALRRWANWTLLALALIFCALAAWIRVRFGEVS
                     FDQIVANLPIGAGKGIGNNSLAVEVAVWCLGLPVVVVVLLALGTRRFRSRLGAKRRAK
                     LIPAVSLAVGFTVLLSVAGVPEFAIAQLSETSIAAHYVEPAVTSVPAKPRNLVTIYLE
                     SGENTYGDAALMGENLLADLDAATGDGWVTYEGLSQYQGGGWTMAGVVSTQCGIPLKS
                     ELASGSVDLNRLGEEIDYYLPGATCLGDVLAGQGYRSVFVGGADDDFAGKGVYLADHG
                     YTEVLGLEHWKGTDSSDFISEEWGLADSHTLKHAADVLDELHAGGQPFNLTVLTLDTH
                     EPAGVYPDCEAEHEVPMASAITCSMKAVAGLLEHAEQAGYLDDTVVMVMGDHLKGTGD
                     QDAFRDVLSAAEKRTVVFRVWSPDGMQLTRESADQLSVLPTTLELLGFGLDAGRAGIG
                     VSFVGSHSIAGTALALSEDAYRELLIAPSTGIYQRFWSAGR"
     gene            complement(15012..15305)
                     /locus_tag="PKJ61_01780"
     CDS             complement(15012..15305)
                     /locus_tag="PKJ61_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016667170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HOC12597.1"
                     /translation="MTTIAPIHPGEILAEEFLAPLEISQNRLAVAIGVPPRRINEIVH
                     GKRRITADTALRLARYFGTTDRFWLNLQTRFDLETEKDQLGTALDQIEPLRTA"
     gene            complement(15317..15598)
                     /locus_tag="PKJ61_01785"
     CDS             complement(15317..15598)
                     /locus_tag="PKJ61_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005936145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOC12598.1"
                     /translation="MIRSFRETATELLWSRKRSRSFDHRIERVALRKLVMLDAAETLE
                     DLRVPPGNRLEALRGDRAGQHSIRFNQQWRICFAWTEAGPADVEIVDYH"
     gene            complement(16145..16230)
                     /locus_tag="PKJ61_01790"
     tRNA            complement(16145..16230)
                     /locus_tag="PKJ61_01790"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16194..16196),aa:Leu,seq:gag)
     gene            16438..17751
                     /locus_tag="PKJ61_01795"
     CDS             16438..17751
                     /locus_tag="PKJ61_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007077792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOC12599.1"
                     /translation="MTSIPSAVDEVAGICSDLIRFDTSNFGPSGAKGEREAAEYVAAK
                     LDEVGIVAELFESEPRRTTLVADWSPEGCDTSLPPLLVHGHTDVVPAIASDWQVDPFA
                     GEIIDGYVWGRGAVDMKDFDAIILAVVRDRMRTGRAPRRPIRLVFTADEEAGGPLGAT
                     WFANNHPELIADCTEAIGEVGGFSISVADQRLYLIQTAEKGLAWLDLIAEGTAGHGSM
                     RSDDNAITRLAEAVTNIGQYSWPARIHPAQRQFLDAVEQALGIQVNADDAETTLARLG
                     SIARMVGATMSNTANPTMLNAGYKHNVIPGHATAGVDGRFLPGYEQEFYDTIARLLPD
                     KVRYEVSISEVAVETTFDGDLVDAMTASLLTQDPGAVAVPFIMSGGTDAKAWSRLGMR
                     CFGFAPLRLPPELDFVALFHGVDERVPTESLEFGCRVLDDLLDRA"
     gene            17748..18476
                     /locus_tag="PKJ61_01800"
     CDS             17748..18476
                     /locus_tag="PKJ61_01800"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012918164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOC12600.1"
                     /translation="MNPDPERARSFGAAVDAYEVGRPDYSAESVAWLLEGAPGRVLDL
                     GAGSGKLTRAVLDLGRDVVAVDPDAAMLARNPAPDTHLGTAEAIPLPDSSVAAVVVGQ
                     AWHWFDPNSAGAEIARVLVSGGRLGLVWNTRDRSHPFVAAVAEAIGASPAEDMVDADE
                     VLALAGFTPWQRRQWQRVRWMSAGEVIAMATSRSAYLIACPAEQRAVVDQLTELLVTH
                     PHSRGRERFEYTLNVTGYRCDRLR"
     gene            complement(18641..18838)
                     /locus_tag="PKJ61_01805"
     CDS             complement(18641..18838)
                     /locus_tag="PKJ61_01805"
                     /inference="COORDINATES: protein motif:HMM:NF024769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HOC12601.1"
                     /translation="MGGDLASELTTALDANPAARATWDAFPPAAKKMALASIALAKQD
                     ATRTRRIAGIVAKAERGERPS"
     gene            complement(18811..18933)
                     /locus_tag="PKJ61_01810"
     CDS             complement(18811..18933)
                     /locus_tag="PKJ61_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC12602.1"
                     /translation="MGRMADAERFHPDELVDWHNWLAANHDRGAGVWVVTWRPN"
     gene            complement(18957..19949)
                     /locus_tag="PKJ61_01815"
     CDS             complement(18957..19949)
                     /locus_tag="PKJ61_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002514231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOC12603.1"
                     /translation="MRQRLVGASGLAVSRLGLGTLTWGRETQLDDARPLLRAFVAAGG
                     NLVDTAAGYASGDAERMLGRMLRAGDVAREEVVVATKAGFGIRDGERRVDTSRAALLT
                     DLRGSLRRLGTDHIDLWQLHAWGQAPLDESLAAIDTAVGAGDVRYAGVCNFVGWQTAQ
                     AATWQAAFPGRTPLTSSQVEYSLLARRAEVEVIPAIRAFGMGLFPWSPIGRGVLTGKY
                     RSGLPRGSRGASEHFAWFVEPYLEARSRSVTDAVCTASAGLDLTPAQVALLWVRDAPG
                     VTAPLLGARTVAQLEPYLATEELELPEEIVAALDDVSGGPNVMRDLGEPTLTLR"
     gene            complement(19946..20788)
                     /locus_tag="PKJ61_01820"
     CDS             complement(19946..20788)
                     /locus_tag="PKJ61_01820"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002529385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HOC12604.1"
                     /translation="MEWWHAILLGIIEGVTEFLPISSTGHLTVAEKLLGFEIADPSVT
                     AFTAIIQIGAMVASIIYFWGDIVRFATAWFRGLAKPEAREDPDYRMGWAIIVGFVITA
                     AIAVLLKDLIEGPLRSLWAVVAGLLGWSVVMFLADRLGKAVRDEHSITWRDGALLGLI
                     QAVSLVPGVSRSGATISGGLFRGLDRVSATRMSFFLGIPTLIASGAFQAVTEASNIGA
                     PGGVGWMATILATIVSGVVAYASIAWLLKFVASNDFTAFIIYRVALALVISALLLTNT
                     IPAV"
     gene            20878..22104
                     /gene="mshC"
                     /locus_tag="PKJ61_01825"
     CDS             20878..22104
                     /gene="mshC"
                     /locus_tag="PKJ61_01825"
                     /EC_number="6.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020384910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010125 - mycothiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--1-D-myo-inosityl
                     2-amino-2-deoxy-alpha-D-glucopyranoside ligase"
                     /protein_id="HOC12605.1"
                     /translation="MRAWKSVVVPRLRGTGSGRVHAHDTLTGQLQPVGPEVGEARLYA
                     CGITPYDATHLGHAATYVAVDLLHRAWLDAGLTVRYAQNVTDVDDPLLERATATGADW
                     EQLAADQIELFRTDMAALRVLPPAELTGVVESLDRVTDLLGVLRDERAIYQVPDPEYP
                     DWYFSVTKSSGLMDGTGYSMLEAEQIFAERGGDPDRPGKRHRLDCLVWRQERNGEPAW
                     DSWLGRGRPGWHIECTAIALGALGTDFDVQAGGRDLAFPHHPMCAAEARVATGHPFAK
                     VFLHTGMVGLDGEKMSKSRGNLVFVSRLLAEGVDPMAIRLVLLANHYRSDWEYTSDVL
                     AAAEARLAKWRTAVARPGADAGPVIEAVRNALRNDLNAPAALAAIDAWADAPGDAADA
                     SDLVREAVDALLGVAL"
     gene            22370..22636
                     /locus_tag="PKJ61_01830"
     CDS             22370..22636
                     /locus_tag="PKJ61_01830"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002527222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HOC12606.1"
                     /translation="MSKSFEDLYAELRERAATRPEGSGTVRELDAGVHAIGKKIVEEA
                     AEVWMAAEYQGKAEAAEEISQLLYHLQVLMVSLGLELEDVYEYL"
     gene            22647..23501
                     /gene="hisG"
                     /locus_tag="PKJ61_01835"
     CDS             22647..23501
                     /gene="hisG"
                     /locus_tag="PKJ61_01835"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016665647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HOC12607.1"
                     /translation="MTGLLKIAVPNKGSLAEGAAGMLREAGYRQRADAKELVLVDDAN
                     GVEFYYLRPKDIAVYVGEGTLDLGITGRDMLLDSGAEAEEVKSLGFGGTRFRFAAPGG
                     ATMSVADLTGKRIATSYSGLVASYLDGHGVTARLIHLDGAVESSVRLGVADVVADVVE
                     TGTTLKRAGLELFGDAILESEAILIQRKGVPAPEGLDLLVRRLSGVITARDYLMVDYN
                     VPRELLEAATKVTPGLDAPTVSPLAKDGWVAVRAMIPAKQAQRIMDALWEAGADAILV
                     TKIHACRL"
     gene            23511..23933
                     /locus_tag="PKJ61_01840"
     CDS             23511..23933
                     /locus_tag="PKJ61_01840"
                     /inference="COORDINATES: protein motif:HMM:NF022216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HOC12608.1"
                     /translation="MVFRPRRSLLMASVLSAVLALAALIGWVTLPPDIQVLFTVPQLL
                     TLAFFALFMIGFMMSIGLSYLAVDDAGLRFRNGVRTHRLPWGEVRGFRFTEHDPWAYV
                     LLDEELQRPLMGIQRTDRELAEADFAKLVRYWHDRTAS"
     gene            23930..24481
                     /locus_tag="PKJ61_01845"
     CDS             23930..24481
                     /locus_tag="PKJ61_01845"
                     /inference="COORDINATES: protein motif:HMM:NF018837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SseB family protein"
                     /protein_id="HOC12609.1"
                     /translation="MSGLRQPSDAYTGDRGEPDATVRASLAAAQQSLDHYLHAIAQLC
                     TTRVLLPIVPVPDPDGSRLAGVGRIEPEHPEREDPAEVHAELVAVTLTAPDGRIGLPV
                     FTGADAFAVWQPDARPLPCRLDEAAANALDQGATALLIDIAGPHALVIEGDLLTELAR
                     SRRLVQLDDGGWGWLFAAAEDPD"
     gene            24726..25400
                     /gene="infC"
                     /locus_tag="PKJ61_01850"
     CDS             24726..25400
                     /gene="infC"
                     /locus_tag="PKJ61_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013161275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOC12610.1"
                     /translation="MNERIHVNEVRLVGPAGEQVGIVRIDDALRLAREADLDLVEVAP
                     MARPPVAKLMDYGKFKYEAAQKARESRRNQTNTVIKEMKLRPKIDSHDYETKKGHVVR
                     FLKAGDKVKITIMFRGREQSRPELGFNLLKKLADDIVEDGFIESAPKQDGRNMIMVLS
                     PTRKKTEAKVEVEAAKAAKAADRAAKTEAERQQEAEIRAAHAAADTKPKKKRGPADNM
                     DPDIDL"
     gene            25435..25641
                     /gene="rpmI"
                     /locus_tag="PKJ61_01855"
     CDS             25435..25641
                     /gene="rpmI"
                     /locus_tag="PKJ61_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HOC12611.1"
                     /translation="MPKMKTHSGAKKRFRLTGTGKVMHRQAGKMHLNEHKPTSQTRRL
                     DGDKVVAKGDAKKARRLLGSYKAK"
     gene            25681..26049
                     /gene="rplT"
                     /locus_tag="PKJ61_01860"
     CDS             25681..26049
                     /gene="rplT"
                     /locus_tag="PKJ61_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007077654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOC12612.1"
                     /translation="MARVKRSVNAQKKRREVLDQASGYRGQRSRLYRKAKEQLLHSYT
                     YAYRDRRARKGDFRKLWIQRINAACRAEGMTYNRFINGLKNAGVEVDRKILAELAVND
                     SVAFAALVQVAKENQPAQVA"
     gene            26065..26928
                     /locus_tag="PKJ61_01865"
     CDS             26065..26928
                     /locus_tag="PKJ61_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020577505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOC12613.1"
                     /translation="MSVASIELPEPSQAVLRAARALQRRQERKETGKFLVEGRQAVRE
                     ALRLPGVVDAVYFRWDAAIDNSDLLDQAKAAGVAYYGVSEQNLATMTDTVTPQGVVAV
                     ANAVDVPLKAVVGKKPKDSDKPKKKHRRKDVSLVVICAQVRDPGNAGTVIRCADAFGA
                     DAVILSSDSVEVYNPKVVRASVGSIFHLPIVVGVDLGEAIEACRKAGMQIFATDGAAG
                     TDLTDLDADLEKPTAWVMGNESWGLPVEHLALADHTVAVPIYGQAESLNLATAAAVCL
                     YASASAQRASN"
     gene            26949..28037
                     /gene="pheS"
                     /locus_tag="PKJ61_01870"
     CDS             26949..28037
                     /gene="pheS"
                     /locus_tag="PKJ61_01870"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015582522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOC12614.1"
                     /translation="MSGEQAELSQSAIDEMVAGALAAFAAAGSSAELKAVRLAHTGDK
                     APLALANRAIGSIPGPERKEAGQRLGVARGQVAAALAAREAEIGAAELERQLITERVD
                     VTLPVELAPEGARHPITALMDLMGDVFTAMGWEIAEGPEAEGEWLNFDALNIGPDHPA
                     RGTTDTLFVDPIEQHIVMRTHTSPVQIRSMLDRELPIYVVCPGKVYRADEFDATHVPV
                     FHQIEGLCVDKGISLAHLKGTLDHLARSMFGDEVETRMRPHYFPFTEPSAEVDLKCFV
                     CKGESVGNPDAPCRTCKSQGWIEWGGCGVVNPRVLAACGIDTEVYSGFAFGMGIERTL
                     MFRNNAADMRDMVEGDVRFSRSLKGMAR"
     gene            28034..30682
                     /locus_tag="PKJ61_01875"
     CDS             28034..30682
                     /locus_tag="PKJ61_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020391001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HOC12615.1"
                     /translation="MKAPLSWLAEHVELPAGITGRELGEVLIRAGLEVEKVETVGAEI
                     TGPVVVGRVLATVDEPQKNGKVIRWVQVDVGPEHNEPHPDYPQGCRSVVCGALNFAVG
                     DLVVVSLPGAVLAGGFAIAARKTYGHVSDGMICAEDELGIGHDHTGIIVLPAVDDSGR
                     ALQPGDDPAGVLHLRDDVLDIDVSPDIGYCLSIRGLARESAQNLGVAFTDVINRPTPA
                     AKADGYQVRLDDPACPLFVALSVAGIDPSRPTPRWMARRLVMAGMRSISLPVDITNYV
                     MLETGQPLHAYDASLLRGPIVVRKAAAGETLVTLDDVTRTLDPDDLLITDASGPIGLA
                     GVMGGQSTELRDTTTDVVVEAANFEAVTISHTLRRHNLPSEASKRFHRGVDPNAAYSA
                     AHLAASLLVELAGGTLSDAETVAGAVPAMPEQVIPSDLPSKILGVDVPAERVVEILLA
                     SGVTVTDEPTVAAGQSQPTVSPTSQPTVIPASQPTVIPAPQPTVIPAQAGISGRALKL
                     TPPTWRPDLVDPYDYVEEVGRKVGFDQIPSIVPPAPVGRGLTRDQAARRAVGHALATS
                     GFVEVLTFPFGSSEDLDRLGLAGADPRRALVRLANPLAETQPYLRTTILPGLLAAVNR
                     NTSRGNDDLALFEAGRVFRAVSSDPAPIPAVTGRPSDAELAAIDAVLPDQPRLLAAVL
                     TGQWRRAGWQGAAVPAGWTQAFAFADIAAKTVGAAVERRSAEFAPWHPGRCAELVVAG
                     EAIGHAGELHPAVVAAFGLPARTAAVELDLDALVRLAPVGGSILPVSGHPVAKEDVAV
                     VVPNEVGAGEVQQALIEGAGELLETIALFDVFTGGQVPEGHRSLAFALRFRASDRTLT
                     DVETAAARDAAVALAAARHGAVLRSF"
     gene            31165..31944
                     /locus_tag="PKJ61_01880"
     CDS             31165..31944
                     /locus_tag="PKJ61_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002549829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HOC12616.1"
                     /translation="MRNVRDIECWLTDMDGVLVHDELPVPGAVELLNRWVDTSRRFLV
                     LTNNSTYTPRDLAARLARSGLPVPEENLWTSAMATGAFLAGQRGAKTAFVIGEAGLTT
                     AMHEAGFVMTETDPAYVVIGETRTYSFEAITKAIRLINDGARFIVTNPDTTGPSPSGL
                     LPATGAVAAMITAATNRQPYVVGKPNPMMFRSAMNRIQAHSETTAMIGDRMDTDIVAG
                     MEAGLLTVLVLSGVTSEPEIEVYPYRPDQVYGSVADLVPLI"
     gene            complement(32233..32892)
                     /locus_tag="PKJ61_01885"
     CDS             complement(32233..32892)
                     /locus_tag="PKJ61_01885"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HOC12617.1"
                     /translation="MRAPGGLAKLLTASEAEVVEAQFTLPEALPVDDFRPAVAAGLDW
                     LVISSVTTVNVLVANGFGAAELRPPGARVAAGGPATAAALRAVGLSPDLVADPGGGVA
                     LVAAGLAELGWQVRPVPVYQTVTQALPAAVVADWQAGRFDAFVVTAGSVARAAVKAAG
                     LPGPQVIAIGEPTAAASRAVGLVVVAVAAAPTTEALAAAVHEALSPRACCSAEHGWRL
                     V"
     gene            complement(32983..33174)
                     /locus_tag="PKJ61_01890"
                     /pseudo
     CDS             complement(32983..33174)
                     /locus_tag="PKJ61_01890"
                     /inference="COORDINATES: protein motif:HMM:NF015835.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
     gene            complement(33206..33502)
                     /locus_tag="PKJ61_01895"
                     /pseudo
     CDS             complement(33206..33502)
                     /locus_tag="PKJ61_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017935972.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
     gene            33557..34588
                     /gene="argC"
                     /locus_tag="PKJ61_01900"
     CDS             33557..34588
                     /gene="argC"
                     /locus_tag="PKJ61_01900"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012923003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HOC12618.1"
                     /translation="MTVRVAIAGATGYAGGELLRLLLAHPEVEIGALTAGSSAGTPLL
                     AHHQHLTPLADRVVAETTAANLADHDVVFLALPHGTSAEIAAQLGPDVLVVDAGADHR
                     LSDPEQWRKFYGSEHAGTWPYGLPELPGQRAQLAHTSRIAVPGCYPTTVTLALLPALV
                     AGLVTGADVVAVAASGPSGAGKALKPHLLGAELMGSASAYGVGGVHRHVPELLQNFTR
                     LGAVEPSVSFTPVLVPMSRGILATVTAPLARPVSAVEAREVYASAYLAEPFVTVLPEG
                     LWPQTQSIVGSNAVHIQVAIDADANRLVAVAALDNLTKGTAGGAIQSMNLALGLPETL
                     GLTTIGVAP"
     gene            34585..35742
                     /gene="argJ"
                     /locus_tag="PKJ61_01905"
     CDS             34585..35742
                     /gene="argJ"
                     /locus_tag="PKJ61_01905"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004811007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HOC12619.1"
                     /translation="MSVTAAKGFTAAGVTAGLKASGKPDLALVVNEGPDAHAAAVFTS
                     NRFTAAPVQWSQQAVANGRLAAVVLNSGGANACTGSAGLADAQQMAEWTAAEVGCDVA
                     DVAVCSTGLIGVRLPMDAVLTGIQLAAQDLANTGGPSAAAAIITTDTVAKESIHTSPD
                     GWTIGGMAKGAGMLAPALATMLVVITTDAVVEPAQLDEALRTATGFTFDRTDSDGCMS
                     TNDTVLLLASGAARVQADPAEFSEALTGVCADLAKQLLADAEGSAHDIEVRVEGAASE
                     ADALAVARAIARSNLFKCAIFGNDPNWGRVLASAGTVPAAYDPEHVDVAFNGVRVFVD
                     GQLGADRSEVDLTKGREVLVEVNLHAGEAAAAIWTNDLTYDYVKENAEYST"
     gene            35739..36677
                     /gene="argB"
                     /locus_tag="PKJ61_01910"
     CDS             35739..36677
                     /gene="argB"
                     /locus_tag="PKJ61_01910"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HOC12620.1"
                     /translation="MIDAGDKAAILIEALPWLEQYVGKTIVVKYGGNAMVDDSLKAAF
                     ATDIVFLRMVGVKPVVVHGGGPQISSMLKRLGIESEFRGGLRVTTPEAMDVVRMVLMG
                     QVNRELVGLLNAHGPLAVGLSGEDAGLLTAERKGLHVDGEEIDLGLVGEVTEVRPEAV
                     VDLINAGRIPVVATVAPDEDGQVHNVNADTAAGALAAALGAERLIVLTDVEGLYRDWP
                     NSTEVIREISSTDLSELLPTLASGMIPKMEACLQAVRAGVPKATVIDGRVAHSLLLEI
                     FTDEGTGTMVVDHAQPRPGTPVRLVEPVETTGVPTS"
     gene            36674..37936
                     /locus_tag="PKJ61_01915"
     CDS             36674..37936
                     /locus_tag="PKJ61_01915"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020391014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HOC12621.1"
                     /translation="MTITTLGSQAELQARYSGTLMNTFGAPKRVFVRGEGAYLWDADG
                     NRYLDLLAGLAVNALGHAHPQVLGSISAQLSTLGHVSNFFATPTQIALAERLAGFVRA
                     NSPGLPARTFFTNSGTEANEAGFKITRMTGRKKLIAMSGAFHGRSLGALALTANPAYR
                     EPFEPLPGVVEFVPYGDADALAAVLDETVAAVIIEPIQGENGVVEPPSGFLAAARELT
                     SEVGALLWFDEVQTGIGRCGDWLASTASGVTADIVTFAKGLGNGFPIGACVATGPAAS
                     LLGPGSHGTTFGGNPVAAAAGLAVLNIIERDGLLARAVAAGEHLATGVLDLGHSLVAG
                     VRGRGLLRGLVLAEPVAAVVAERALDAGFVINAPRPNVLRLAPPLIVTDAQLDTFLAA
                     LPDLLDAARAAEAPRRVAELHEAPQAPR"
     gene            37933..38421
                     /locus_tag="PKJ61_01920"
     CDS             37933..38421
                     /locus_tag="PKJ61_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008359409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HOC12622.1"
                     /translation="MTEQRTPVTKNARQSRIAELIERETIRSQTELAGRLAAEGLAVS
                     QGTLSKDLLELGAVRVRTSTGELAYALLATDHGGAQSGALARLTRLCGELLLSAEASA
                     NLTVLRTPPGAAQFFASAIDKVGWDSVLGTIAGDDTVVIVTRAPDGGAAMAEAFLSLH
                     PS"
     gene            38433..39827
                     /gene="argH"
                     /locus_tag="PKJ61_01925"
     CDS             38433..39827
                     /gene="argH"
                     /locus_tag="PKJ61_01925"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014847018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HOC12623.1"
                     /translation="MTEATDAGRLWGGRFAGGPADAMFALSQSTQFDWRLARHDLAGS
                     KAHAKALHAAGLLTDTELGGMLTGLEALDADVASGAFTPDPTDEDVHGALERGLIAKV
                     GLDLGGRLRAGRSRNDQIATLIRAYLRDANADLAGDLRALVSALADQAEANLAAIMPG
                     RTHLQSAQPILLSHHLLAHAWPLVRDLQRLADLGNRLAISPYGSGALAGTSLGLDPEL
                     VAAELGFTGSAPNSIDGTAARDLVAEEAYVLAQIGVDLSRLSEDVILWCTHEFGFAKL
                     ADAWSTGSSIMPQKKNPDVAELARGKAGRLIGNLAGLLATCKGLPLAYNRDLQEDKEP
                     VFDGLDQLRILLPAVTGMVATLTFDHARMAELAPRGFSLATDVADWLVRQRVPFARAH
                     EIAGACVRYCEARGQELSDLTVPQLAEISPELGEGVLAVLSAAGSVASRNGRGGTAPD
                     QVRSQLAELRAAIA"
     gene            complement(40104..40436)
                     /locus_tag="PKJ61_01930"
     CDS             complement(40104..40436)
                     /locus_tag="PKJ61_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC12624.1"
                     /translation="MPSFRASAAIIAARPGVPPPAVLAAARTAVALGWQVEDAFVDVE
                     PLARGSGPPRATIRFLVPAANDAEEEQVAWAAAKALAGGLAPVATWTDLRVSRRQRGR
                     WVPVDNPS"
     gene            40510..41775
                     /gene="tyrS"
                     /locus_tag="PKJ61_01935"
     CDS             40510..41775
                     /gene="tyrS"
                     /locus_tag="PKJ61_01935"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002527237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOC12625.1"
                     /translation="MNAVLEELTWRGFIAHSTDPDALGAAFDAGQVSSYVGFDPTAPS
                     IHMGNLVQLMLARRLQAAGHRPFLLVGGSTGLIGDPKQSSERNLNPRELVHEWVERIR
                     SQVSRFVDFDGPAAAQVVNNYDWTAELSTLDFLRNIGKHFSVNRMLDREVVKARLESG
                     ISYTEFSYVLLQSLDYLELFRRYGITLQTGGSDQWGNLTAGVDLIRRAEGAKVHALAT
                     PLLTKADGTKFGKTEAGTVWLDPTMTSPYAFHQFFLNAEDAKVIEYLKIFTVRSEEEI
                     AELARQTAESPHLRAAQRALADDITDLVHSPTERASATAAAAALFGRGELAELDHAVL
                     AALWHELGEQQMAVGPDLPTVVDVLAASGVVASKSAARRAITEGGAYLNNQKIGDPDV
                     VVSAADLLAGRYLIVRRGRRTVGAVELVR"
BASE COUNT         6818 a        14275 c        14571 g         6824 t
ORIGIN      
        1 aggtgcagcg caccttctac gcccgcggcc agaccggcca gcagctgctg atcggtgcct
       61 accaggcgct ggagcgccag gtcgcggccc agacggtgaa gatgtacacc cgccacgaaa
      121 tgctcgacct gatcgtcgtg gacggacgtg cgcgcggcat cgtgacccgc gacatggtca
      181 ccggcgcgat cgagagctgg acggccgacg cggtcgtgct ggccaccggt ggctacggca
      241 acgtgttctt cctttccacc aacgcgatgg gctgcaatgt caccgccaac tggcgggcgc
      301 atcgcaaggg tgcctacttc ggtaacccct gctacacgca gatccacccc acctgcatcc
      361 cggtgcacgg cgagacccag tcgaagctga ccctgatgtc ggagtcgctg cgcaacgacg
      421 gccggatctg ggtgccgaag aaggccgagg actgcggcaa ggacccgcgc cagattcccg
      481 aggaggaccg cgactactac ctggagcgga tctacccggc gttcggcaac ctggtgccgc
      541 gcgacatcgc gtcccggcag gccaagaaca tgtgcgacga aggccgcggt gtcggtccgg
      601 cggtcaagga gttcgaccac gacggcaacg aggtgttcac ccggcgtggt gtctacctgg
      661 acttcgcgga cgcgatcggc cggctcggca aggacggcgt ggctgcccgc tacggcaacc
      721 tgttcgatat gtacgcccag atcaccggcg aggaccccta tgaggtgccg atgcggatct
      781 acccggcggt gcactacacc atgggtggcc tgtgggtgga ttacgacctg atgtcgtcga
      841 tcccgggcat gtacgtcacc ggtgaggcca acttctccga ccacggcgcg aaccgcctcg
      901 gcgcgtccgc tctgatgcag ggtctggccg acggctattt cgtgctgccg aacaccatca
      961 acgactacct cgccggtgcg gcgtccttcg gcaaggtcga cgacagccac ccggccgtgg
     1021 tcgcggcggt cgccgagact acggagcgga tcgagcgcct gctcgcggtc aagggcaccc
     1081 gcacggtcga ctccttccac aaggagctgg gccacatcat gtgggagtac tgcggcatgg
     1141 agcgctcgga ggagggcctg aagctcgcga tcacgaagat cgatgagctg ctggaggagt
     1201 tctggcgcga cgtcaaggtg accggcgtca acgaggactt caaccagacc ctcgagcggg
     1261 ccgggcgcct gctcgacttc ttcgagctgg gccggttgat gtgcgtggac gcgttgcatc
     1321 gccgcgagtc ctgcggcggt cacttccgag ctgagagcca gaccgaggac ggcgaggccc
     1381 tgcgccacga cgacgagttc ctctacgtgg cggcctggga gtacgccggc accggggcca
     1441 aacccgtcct gcacaaggaa ccactggtct acgaggccat cgagctaaag caacggagtt
     1501 acaagtgaac atcaccttgc gcgtatggcg ccaagctaac gcccagaccg aagggcggat
     1561 ggttacctac caggtgcaga acgtctcttc ggacatgtcg ttcttggaga tgctcgacct
     1621 cctcaacgag gacctcacca tcaagggtga ggagccggtg gccttcgacc acgactgccg
     1681 tgagggcatt tgcggcatgt gtggcgtggt gatcaacggg acggcccacg gccgcggcga
     1741 ttcgccggtg cccaccacca cctgccagct gcacatgcgg tcgttctcgg acggcgccac
     1801 gatcgacgtg gagccgtggc gcgccggtcc cttcccggtg atcaaggacc tggtcgttga
     1861 ccgctcggcc ttcgaccgga tcatccaggc cggtggcttc atctcggcca acaccggtgc
     1921 agccccggac gcgcacgccg cgccggttgg caagctgaag gccgatcgcg ccttcgacgc
     1981 ggccacctgc atcggctgtg gcgcctgtgt ggcggcctgc ccgaactcct cgggcatgct
     2041 gttcacggca gccaagatca cccacctggc gatgctcccg cagggccagc cggagcggta
     2101 cagccggatc aagtcgatga tcgcgaccca cgatgccgaa ggcttcggtg gttgcaccaa
     2161 catcggcgag tgcgccgagg tctgccccaa gcagatcccg ttcgacacga tcagccagct
     2221 caaccgcgac ctgatcgcct cgctgttcaa gaaggacggc aagtaacagc cactccagcg
     2281 gcggcgtcac cctctgtggt ggcgccgccg ctggcgttct ggcgcgctgg ccctgcgccg
     2341 atttccggcc ggacggccag ctatggcaga ctggacgact gttgtgccgt gggcgctcct
     2401 gccacagggg gaaggcggcc acgaccatcc aaaagcatct agacgaagtg acctgtggca
     2461 ctggcgagga aatcatgaat cagttggttg agcaggtcgg caaggcagcg ctccgcgacg
     2521 acgtgccgga cttccgtccc ggcgacagcg tcaaggtgca cgtccgagtc attgaaggca
     2581 accgctcccg gattcaggtc ttcgccggcg ccgtcatctc gcgcaccggc tcgggtatcg
     2641 ccgagtcctt caccgtccgc aaggtgagct tcggcaccgg cgtagagcgc accttcccgc
     2701 tgcacagccc gatcatcgag aagatcgagg tcgagcgtcg cggcgacgtg cgtcgcgcca
     2761 agctgtacta cctgcgcggc ctgcgcggca aggcagccaa gatcaaggag aagcgcgacc
     2821 gctgagtcgt cggcgtcctc tatcgcgcat ctgggatact ggagttcgag aggggaccgc
     2881 gatgagcgca ccgacttcag ccggcaatgg gcgcaaagcc tggtcggtga tcggcagttg
     2941 gcttcgcgag atcgccatcg tggtggtcgg cgcgctggtg gcttccacgt tgctacgtct
     3001 gttcgtcgtg cagatgttcg tgatcccgtc cggctccatg gagaacaccc tgctggagca
     3061 cgaccgggtg gccgtgcaga agatcgtggg cttcgagcgc ggcgatatcg tggtgttccg
     3121 cgacacccag aactggctca ttccggtgcc ggtgaaggac gaccccgttg tgaagacgct
     3181 cgtgttcctc gggctggcgc ccgatgagag cactggacac ctgatcaagc gggtcatcgg
     3241 cgtggccgga gaccatgtcg cctgctgcga cgccagggga cgcgtcaccg tcaacggcgt
     3301 ggcgctgaac gagactgagt acctgtacac ggatccagcc actggcaagc aggtcgatcc
     3361 gtcctcggtg gccttcgatg tggtggtgcc ggccggtcgg gtgttcatga tgggcgacca
     3421 tcgcaacgat tcgcaggact cccgctgtca tctcgatgag caactcgatt ccgatccagc
     3481 gggcagcgcc gcgttcatcc cggtcgagaa catcgtgggt accggagtgg ccgtggtgta
     3541 cccgttcgca cggatgcaca cgatcagccg tccgctcacc ttcgagagca ttcctgcggg
     3601 acaggatccg ccgacggaag ccgtgatcac cggccccaag gttctctgct gaggtcgagt
     3661 tggcggtcgt ggtacgccgg gacgccggca tctatgccta cgaggcaacc ctttcccgag
     3721 cgggtttcgg gctgatcgcc ggcgccgatg aggccggacg gggcgcctgt gccggtccac
     3781 tggttgccgg agccgtgatc ctgcgtctgg gggccgcggg cgagattccc ggactggccg
     3841 attcgaaact gctcagcgcg gcccagcgcg agcgggtgta tgccgaagtc actgcgcgcg
     3901 cactgagttg gtcagtggtc tgtgtcgagg ccgacgaatg cgacctgctc ggcatgcatc
     3961 acgccaacct caccgcgctg cgccgggcgt tgctgcggct cgagccgacg ccggcgttcg
     4021 cgctcaccga cgggttcggc gtggatgggc tgggcgtgcc gaacctggcc atctggaagg
     4081 gtgaccgggt ggcggcgtgc atcgcagcgg cgtccgtggt ggcgaaagtg acccgcgacc
     4141 ggatcatgac ccagtggcac gagtcgctgc cgcattacgc attcgacgtt cacaagggat
     4201 attgcacgga tctgcatcag gagcggctcg accagcacgg cccgagcccg atccaccgct
     4261 ggtgtttcga gaatgtgcgc aggacagcgt cggcgtccgc ttgcgagacc tcgggcgatg
     4321 gcagcgcgcc ggacgcggcg gtcgtccggg aagccacaac cacggcggct ccactcctcg
     4381 atatagagtg aatccgacat gagcaccgaa gaccttgagc agtacgaatc cgaactcgaa
     4441 ctgcagttgt acaaggagta caaggacgtc gtggggatct tcacctacgc cgtggagacc
     4501 gagcgacggt tctatctgtg caacgcggtc gacctgaagg tgcgtaccga gggtggagat
     4561 gtctactacg aggtgtccat gggtgacgcc tgggtctggg acatgtaccg cccggcccgt
     4621 ttcgtgaaga gcgccaaagt gctcaccttc cgcgacgtgt cgatcgaaga gatcgcgcac
     4681 agcgagttga aggttcccga caacctctga atggggcgaa gctcggtcgt cccggcgccg
     4741 gttccgtcat cacgctcggg ctcggccatc ccgcgagggc tcgtcctggc gagggttcgg
     4801 ccatcccgcg agggctcagt catcccggcg aaggccggga tctcccgcgg ccagagtcgc
     4861 cgcgccctgt ggataacgca attgctatcc acaacccact gattcgttga cagatttccg
     4921 gcgcacttgc cgaaggtgta gctggaggtg atctgtgtgg gacgagccga ggtgtggcat
     4981 gcgggggagg atctcgcggc cgcgcacctg cagcggctgg gctggcaggt gctcgaccgc
     5041 aactggacgt gtccggtcgg cgaactcgac atcgtcgcga tcgagccggg tccggaagcc
     5101 gtcgtggttt tctgtgaggt gaagacgagg cgtgggctgg gattcggcac accgctggag
     5161 gcgatcacac cggcgaaggt ggcgaagctg cgtgaagtgg cgctgcactg gctacgtgcg
     5221 cggcccgtgc cggtgccgca catccgcttc gacggtctcg gcgtgctgct gccaagggac
     5281 gcgcaaccgg tgatcacgca ctgccggggc atcgggtcgt gagacaggcg agagcatggt
     5341 cggtggcgct ggtgggcatc gagggccgaa tggtggaggt ggaagcggcc atcagcagtg
     5401 ggctgccgcg cacgaccatg gtcggcttgc ctgacgcggc gctctacgag gcacgggacc
     5461 gctgtcgtgc ggcgatggcc gcagtagggc cgggttggcc gtccgatccg gtgacgatca
     5521 acctttcgcc tgcgacgctg cccaaggccg gttcccacta cgatctggcg atcgcggctg
     5581 ccgtggccgc cgccggccgt aggttccacc ccgacgcgct ggagggtttg gtgctgttcg
     5641 gcgaactcgg tctggacggc cgcatccggc gggtccgggg gttgttgccg gcactgctgg
     5701 cggcgatcgg ccagggcttc ggcagggccg tggtgccggc gacccagctt cgtgaggctg
     5761 cgcttgtcga cggcctggtt gcgtacgggg cgaaccgact cgacgacctc ttcgacatcc
     5821 tggaaggtgg cccgggcgcg gacatcacac ctgccgaacc caccgtagat ccggtgccca
     5881 ccgggcggga cctagccgat gtggccggcc aggtcgaggg caaatgggcg ctggaggtgg
     5941 ccgccgccgg gcggcaccac ctgtacttcc acgggccgcc cggggtcggg aagacgctgc
     6001 tggccgagcg gttgcccacg atccttcccg acctggagct ttgggaggcg ttggaggtct
     6061 ccgcggtgca ctcgcttgcc ggtctggccc tcgacgaagg actggtccgg cggccgccct
     6121 actccgaccc acaccactcc gcatccatgg cgagcctggt cggcggcggg gcccggatcg
     6181 ccatgccggg cgcggtgtcc agggcgcacc ggggcgtcct cttcctggat gaggccccgg
     6241 agttctcgcc ccgggtgatg gaggcccttc gagtgccgct cgaatccgga cgcatcgtgc
     6301 ttggccgggc ggccgcgacc gccacctatc cggccagctt ccaactggtg ctggcggcga
     6361 acccctgccc ctgcggcatg gccggcacgc cgggcggcga ctgtcgctgt gcgccgatgg
     6421 cggtgcgaag atacgcagcc agggtgtccg ggccgatcct ggatcggatc gacatccacc
     6481 agcagttgcg gcccgcccgg gcgtcctttc tgggaaacca agtggttggc gagaacagca
     6541 cgaccatcgc ggcgcgagtg gccgcggccc gggaacgaca gctccatcgg ttggccgcca
     6601 gtggatggcg cagcaacggt gaagtcccgg gtccagctct gcggaagcgg ctgccactgc
     6661 cagacggggc agaactgctg gacaccgcgg tcgcccgggg ccggctgtcg gcgcgaggtg
     6721 ttgacaaggt acttcgcctc gcctggacgg tggccgacct gcaggggagc gcgaagccga
     6781 gtcgggagca tttgcacaca gccctcgcga tgcgccgggg cgagacgttg gaggcgaagt
     6841 gacggacgaa cgaaccgcgc ggatgtgcct ggccgcggtg gtcgagcccg ggcaccccac
     6901 gatcgcggag gccgtgctca gctacggggc ggctgcggtg tgggcgagcc tcacccgtcc
     6961 tggtgccgat ctgccgctga gccgtcgggc acgcgtcctg gtggtcgagg acttgctcac
     7021 cgcgaccaga gcgctcggca tgcggttcgt ggttcccggc gacgcggagt ggcccggcgg
     7081 caccgaggat ctgcgcagct gtgagccggt gaaccagctc accggtgaac ccctggggct
     7141 ctggttgcgc ggcgcgggtg acctggccga gttggccggc accagcgtcg cgatcgtcgg
     7201 ctcgcgcgcg tccacgccgt acggcgacac cgtggcggcc gatctgggag ccgagctctc
     7261 ggaggcgggc cggagcgtca tctccggcgg ggcctacggg gtggacgccg ccgcgcatcg
     7321 cggttgcctg gcaggtcgca gtccgacggt cgcagtgctc gccggcgggc tggatcaggc
     7381 gtatccggcc ggtcaccacc agttgttcga gcgcatcgcc gagcgcggcg tgctggtttc
     7441 cgaactggcg cccggcgagc atccgacccg cgtccgcttt ttggcacgaa accgcctgat
     7501 cgccgcgatg acacccggaa cagtgatggt ggaggcggcg gccaggtcgg gcgcccgcaa
     7561 cacggtcacc tgggcgaacg aactcggccg ggtggtgatg gcggttcccg ggccggtaac
     7621 cagcgcaaca tcggtcacgc cgcaccggct gatccgcgaa gccgaggctg tgctcatcac
     7681 tcgggcaccc gacgtgctgg agttgctgtc cccgctcgga cggccgaacc cgcgcatacc
     7741 tggaggccga agggagaccg atgatctgtc cgctgaagag ctccgcctgt tcgagtgcgt
     7801 gcccgggcgg ggctcgctgc cggccgccga actggcccta cgcgcggacc tctcactgcc
     7861 tcgctgcctg gcgctccttg aggagctcgc cgatcgcggt ttcgtggcgc aaaatctcac
     7921 cggagagtgg caattgccgc cacgcgcacg gaagactggc tgagtgctga attgcatgca
     7981 acgtcgcgct ccgctcgagc gtgtagggat catgaagaag ttgggatcgg ccctgatagc
     8041 ggcggcaatg ctcgctctgg tgggctgttc tgcggggtcc ggggccggtg cacgtgctga
     8101 ggacgtgtcc tcctatgcgg gcgaggatgc cagcgcagga ccgcaggcca tgccgacagg
     8161 caccgactcg acgctcacac cgcaagtggc acgctcggcc gaagtgagcc tgcgggtcac
     8221 cgatgtgatg gtcgcggcgc aacggctgcg tgaactggcg agcgccgccg gcgggcaggt
     8281 cactgccgag agcctcgtcc tggacgccaa ggaccagagc tactccgccg tggtcatctc
     8341 cgtcgatgct gacaagctcg acagcacgct gactgcgctg gccgaggtgg gcacggtcga
     8401 gtcgcgggtg atcaactccg aggacgtcac cacccaggtg gcggatgtga atgctcgggt
     8461 cgcggcactg aacgcttcca tccagcggct gcaggaactc tcgaagaagg ccggcagcgt
     8521 cgaggaactg gtccgggttg agaccgagct gtcgaatcgg atcgccgagc gcgactcgat
     8581 ggtggcccag cagaaatggc tgtccggacg ggtagcccag tcgcagatct cagtgcagtt
     8641 gatcacgccc gaacaggcgg gacccctgca gacgacgggg ttcatgggcg gactactggc
     8701 gggctggaac gccttggtcg cgaccggatt gaccttgctc accgtggtcg gcgcgctgct
     8761 gccgttcgcc gcggtcgccc tgctgattgc gctcccgctg ctgttctggc gccgccgcgt
     8821 ccgagcccgc aaacgccagt cggtgccgga tcccccgccc accgtcgact gagctgacgc
     8881 gcgggctcaa tgaacttcga ataggtggga cacgcgctca gattcagcgc gcgggtagcg
     8941 tgcgccgtgg ccccagcccc tagggttacc gggtgcacaa gctgaccgtc gcccaaggca
     9001 ccgcgctgag cgccggcgcg gtcctcggca ccggcatcat ctccatgccc gcgttggcag
     9061 cggctgttgc cggtccggcc tccctgatcg cgtggctcgc tctggtgttg ctttcggctc
     9121 cgctggcctg gacgttcgcg gaactgggcg cacgctttcc cgacggcggc ggtgtctcca
     9181 gctacgtccg ccgcgccttc ggcacctggg cagctgatgc catcggctgg atcttctact
     9241 tcgcagttcc gatcggcgcg ccggtcgcct gctccttcgc tgccggctat gtcgcggacg
     9301 tgctcggcgg gggacaggtg gcccggatcg gtacctttct cggcatcatc gggatcgttt
     9361 tcttgatgaa ctggctgggc ctgcacgtct ccggacgcat gcagttggtc ctcgcgggcg
     9421 tcctggcact ggtcctgctg acgaccgtcc tggccgcgct gccccatgct gacccgggca
     9481 acctcactcc gttcgccccg gcggggctag gcgcgatcgg ctcggcggcc gcgctcctgg
     9541 tgtgggcttt cgcgggctgg gaagccgtgt cgtccctgtc gccggagtat gccgatcccc
     9601 gacgtgacgt gccgagggcc acgatgctcg cggttgtggt cgtgggcgtg ctgtacctga
     9661 gcgtcgcggt gaccagcgtg ctggtgctcg gaccgcagct ggccgccagc caggcgccgc
     9721 tggccgatct gctggccact ggggtcggcg gcccggtgcg tccgatcatc gcggtcgtcg
     9781 cggtactgat gaccctgggt gcagtcaacg cctacatggc tggtgccacc aagctgggtt
     9841 cggcgctcgc cgcagagggt gcactgccgt accggatctt cggcacggat cggaaggcgc
     9901 tgatgttcgt cgtgggcgcc gcggtcgtgt ctgagtggct cctcgcagac cagcacaccc
     9961 tgatgctgct ggtgagcggt gggctgaccc tggtctatgt ggtgggcacg gcggcagcgg
    10021 tgaagctgct gcccggaccc ggcgcgaagg ctgtcgccgc gttctcgctg gcctgcgtct
    10081 cggtgctgct ggtgctcacc gggtggccgg cgctggccgg tctcggaatc gcgatcgccg
    10141 cagttctgca tcgtctctgg gcggaccggc gtaggcggct tgctaagagt tccaagtttg
    10201 gttaataata ctgagcatgg ccctgagtcc gcgtgagcga gagatcctcg cattgttgcg
    10261 cgcacacccg ttgctcgacg cgtccggtct ggcccaacgc ctcggcacca cgaagggtgc
    10321 gatcgcggtg gcattgtcgt cgctgaccca gaagggcgag atcgtcggtc gcggctacct
    10381 gctgcgcagc gatgcctggg cggtcgtcgt cggcggtgcc gcctgggaca tcaaggctcg
    10441 cagcagcgca cccgtccggg tgggcacgtc gaatcccggc acggtcagcc agacccccgg
    10501 cggggtgggc cggaacatcg ccgaaggcat cgccaggctg ggtggccaag tgcacctaat
    10561 cgctgcggtc ggctcggacg gctccggacg cgacctgctc acccgcaccg cagaggccgg
    10621 cgtccatgtc gaccggatga tcgattccac ccatccgacc gcgagctacc tggctgcact
    10681 ggacgccgac ggtgagttgg tggtgggcct ctccgacttc gcggccaccg acgcgctgac
    10741 ggtcgccgac ctggccaggt cgcaggacct gatcgccaac gcagcggtcc tcgtggtcga
    10801 cggcaacatc ccggcgccgg tcgtggattg gctcctcgcc gtggcggccg gttctggcgt
    10861 ccgcgttgtg cttgagccgg tcagcgtggc gaaagccgga cgcatcgccg gcctgctcag
    10921 ccccgaccga ccggtcttcg cgatcacgcc caatgtcgat gagctcggtg ccctggtggg
    10981 ccgcccggtc gccgacaccg aagtagccat cgccgaggcc gccgccgacc tgcactcccg
    11041 tggcgtgagt cacgtctggg tgaccagggg agcgtccggc agtctgctgg tgagcgcgga
    11101 cgcaccagcg ccgcagcgcc tggatgcccc ggccagcgaa gtggtcgatg tcaccggggc
    11161 cggggacgcg atgactgccg gtttcgtcca cggcctgctc atcggcgaga gcccgctcag
    11221 ttctgcccag ctcggccaat tggccgcggc cctgacggtg gccagcaggt tcaccgtccg
    11281 ccctgacctg ggggtggcgc tcgccgacct ccaccgaacc ctcgaaggag tccgatgaac
    11341 ccgctgctcg cactgaccga tgaggtgcgc gaagccctgg cgtccggtaa gccggtggtc
    11401 gccctggagt cgaccatcat cagccacggc atgccgtacc cgaagaatgt ccagaccgcc
    11461 cgcgaagtgg aacagatcat ccgcgacggc ggcgcggtcc cggccacgat cgcgatcctc
    11521 cacggcgtgc cgcggatcgg gctgtccgac gccgagctcg acctgctggg gtctgacgag
    11581 aacgtgcgca aggtgagcat tcgcgacctg ccctacgtgg tggcgcgcaa ggagcacggg
    11641 gcgaccacgg tggcctcaac gatgcgcctg gccgccctcg ccggcatcaa ggtgttcgtc
    11701 accggcggcc ttggtggtgt ccatcgcggc gccgagtcga gcatggacgt ctccgccgac
    11761 ctcaccgagt tgagcctcac cgatgttgcc gtggtcagtg ccggggtgaa gtcgctgttg
    11821 gacatcggcc gcaccctgga ggtgctggag acactcggcg tgccggtgat ctcggtcggc
    11881 tcggacgagt tcccgtcctt ctattcccgc tccagtggct tccgctcgcc gctgcgcctg
    11941 gatgctgcgg cagagatcgc cgcggcgatg cacgccaagt gggaccttgg gctggctggc
    12001 ggcctggtcg tggccaaccc gatcccgatc gaagacgaga ttcctgaggc tgagatcggt
    12061 gccctgatcg cccgggccgt cgccgaggct gacgagcagg ggatcactgg tgcagcgatc
    12121 acgcccttcc tgctcggccg aatcgtcgag ttgtcggacg gcgagtcgct gcgcgcgaac
    12181 atcgccctgg tgaagcacaa cgctcagctc ggcgctgcga tcgcggtcgc ctacgccggc
    12241 tgagtcccct ctcgaccagt cagtagcttc gcccaggctt gaggccttcc atctgggagc
    12301 cggccgcgcg aatcttgagt ggctggatag ggcgggggac ggctatgacg caggcggacg
    12361 cgcgggagcg ggcatcagtt gatgtgctgg ccgaggtggc aaacgacctc gccggggaat
    12421 tccggctgca accgctgctg ggacgcatcc tgcggagcgc ggtcgaactg ctgggttgcc
    12481 gcagcggctc gttgtgcctg atcgaccagg tcagccacac ctaccgcaag gcgggctggc
    12541 cacgatcgct ctccgaatcg cggacgcgca cgcggccgcc agccacggag aggatccgtt
    12601 gcccgcgctc acccaggccc aacaggccgc ccaggggacc atgaacgagg gccggcgtgc
    12661 ggtctggggt caagagcgag cagggtgcga cgctggcgga ggtcagggcg atcggcgaga
    12721 aggtcaactc ggtgacccga accatgggca tcgcgctcac cgcgtgtacg ccgcccgcga
    12781 agggcagtcc gctgttcgat ctcggtgatg acgagatggc ggtcggggtg ggcatccacg
    12841 gtgagcccgg acggcgtcgc gccaagctgg tcagcgccga agagatcgtg gacgagttgc
    12901 tcggggccgt cgtaaccgac cggccgcacg cctccggcga ccgagtggcg ctgatgatca
    12961 acggtctggg cggtaccccg atcagtgagc tctacctgct gttcggcatc gccgcgcaga
    13021 agctggccga ccagggcatc acggttgcac gcagctatgt cggcgaatac tgcaccagcc
    13081 tggaaatggc cggggcatcg ctgaccctgg tcaagctgga cgacgagatc gcgacactgc
    13141 tcgaagcccc tgcgtcgatc gtgaaccgca tcttctgagc cgggaaaccg cttccgggct
    13201 tgtcgactca accaaagctg agtcgacaag cccgaccagc tgatgccact tgtccgggga
    13261 cactgacgca aattcggcgt cacgagccgc tgcgtcgggg ttggtagaat cgctcgacaa
    13321 tgcctcccga caagccacag caccggatcc tgccggccct gcgacgctgg gccaactgga
    13381 cgctgctcgc gctcgcgctg atcttctgcg cgctggcggc atggatccgc gtccgcttcg
    13441 gtgaggtgag cttcgaccag attgtcgcca acctgccgat cggcgcggga aagggcatcg
    13501 gcaacaactc gctggccgtc gaggtggcgg tgtggtgcct cggactgcca gtcgtggtcg
    13561 tggtgctgct cgcgctgggg acgcgacgct tccgctcgcg gctgggggcc aagcggcggg
    13621 caaagctgat cccggcggtg tcgctggcgg tgggcttcac ggtgctcctc tcggtggcgg
    13681 gtgtcccgga gttcgcgatc gcgcagttgt cggagacctc gatcgccgcg cactacgtcg
    13741 agccggcggt gaccagtgtc ccggccaagc cgaggaacct ggtcacgatc tacctcgagt
    13801 caggtgagaa cacctacggc gatgcggcgc taatggggga gaacctgctg gctgatctcg
    13861 atgccgccac cggcgacggc tgggtgacct acgagggctt gagccagtac cagggcggcg
    13921 gctggacgat ggccggcgtg gtcagcacgc agtgcggcat cccgctgaag agcgagctcg
    13981 ccagcggaag tgtggacctg aaccggctgg gtgaggagat cgattactac ctgcccgggg
    14041 cgacctgtct gggcgacgtg ctcgccgggc aggggtaccg gagcgtcttc gtcggcggcg
    14101 cagacgacga cttcgccggc aagggcgtct acctggccga ccacggctac accgaggtgc
    14161 tgggactgga gcactggaag ggcaccgatt ccagcgactt catctcggaa gaatgggggc
    14221 tggcggattc gcatacgttg aagcacgccg ccgatgtgct ggacgaactg catgccggcg
    14281 gccagccgtt caacctgacc gtcctgaccc tggacaccca tgagccggcc ggcgtctacc
    14341 cggattgcga ggccgagcac gaagttccga tggcgtccgc gatcacctgc tcgatgaaag
    14401 ctgtggccgg cctcctggaa cacgccgagc aggcgggcta tctggacgac accgtggtga
    14461 tggtgatggg tgaccacctg aagggcacag gtgaccagga cgccttccgt gatgtcctga
    14521 gcgctgcgga gaagcggact gtggtcttcc gggtttggag cccggacggg atgcagctca
    14581 cccgggagtc cgccgaccag ctgagcgtgc tgcccacgac gctggagctg ctcgggttcg
    14641 gcctggacgc gggccgcgca ggcatcgggg tctccttcgt cggttcgcat tcgatagcag
    14701 gcaccgcgct ggcgctcagc gaggacgcct accgcgagct cttgattgcg ccgtccactg
    14761 gcatctacca gcggttctgg agcgccgggc gctgacgatc tgagggcgaa ctggcccagg
    14821 ctcgggtagc gtgcacgatc ttgaaccgtg ttcggtacag gatcttgccc gttcgctttc
    14881 gacacgaaaa agcccctagt catttgacta ggggcttcgc tccatgcggt cgaccggcca
    14941 tcgttaagcg cgactacacg atgtgcatcc acggctgcgc gcggagcgcc ggtcactcgg
    15001 atcggtgcag ctcaggcggt gcggaggggt tcgatctggt cgagggcggt gccgagttgg
    15061 tccttctcgg tctccaggtc gaaccgggtc tggaggttaa gccagaagcg gtcggtggtc
    15121 ccgaagtagc gtgccagccg cagcgcggtg tcggcggtga tgcggcgctt gccgtggacg
    15181 atctcgttga ttcggcgcgg tgggacgccg atcgcgacgg cgagccggtt ctgggaaatc
    15241 tccaggggcg cgaggaactc ctcggcgagg atctcgccgg ggtggatggg agcgatggtg
    15301 gtcatgggca cctcgttcag tggtagtcga cgatctcgac gtcggccggc ccggcctcgg
    15361 tccaggcgaa gcagatccgc cattgctggt tgaacctgat gctgtgctgg ccggctctgt
    15421 caccacggag cgcttcgagc ctgttgccgg gtggaaccct caggtcttcg agcgtctcgg
    15481 cggcgtcgag catgaccagc tttcgcaatg cgacgcgttc gatccggtga tcgaacgacc
    15541 ggctgcgttt ccgtgaccac aacagttccg ttgcagtttc ccggaaggac ctgatcacga
    15601 caccactata acgcgacgcg tcatggcctg ccagctttcg gcccccggcg ggcttcgaac
    15661 cccggatctg accatctcca agtcgccttt ggaatccact ctcaacatgt cgttgcaacg
    15721 agagcgttat cgcctacggt ggcgaggagg taaggcaatg aacagcgacc ctgcgggcga
    15781 cgcaaccagg gcaggtggcc accgacgccc cgggcaagaa gttctctgcg ggtggtgcgg
    15841 ccgaagcgcc ctgatcccgg cccgcggacg cgtcccgaag tggtgctcgg cgacgcttcg
    15901 cgacgtgagc gacgacatcg cgaccacctg gaacgagccg gccgacgccg tacccatcag
    15961 cctcagcgca tccgcgcctt cggacagcgg cgacactcgc ctcccgctgg tcctgatcac
    16021 acgtgtccac cggtggccat cagagggcca gctgcgccgg cagatgttgt gcatatgttg
    16081 tgcaaggccc cggacatgac gacggccctg actgtgtcac ctagtcagag ccatgttccc
    16141 tgagtgtccg agagaggact tgaacctcca cgcccgataa agggcactag cacctcaagc
    16201 tagcgcgtct acctattccg ccacccggac agggtgccgt ccgggtggtg ccggacaacc
    16261 agagcactat acctaatccg gggcccggtt tgcgaaacgg gccggtcgcg cgtacagagt
    16321 gagccgattc gcgtctgctg tgacgggtct tcgcgactcg ccgacctcca gccattgatc
    16381 ggacgcggtc cgtcccggtc gccgagcgcg aggtgagccg gatggcagga tgggcccatg
    16441 accagcatcc cgtccgccgt cgacgaagtc gcgggaatct gcagcgacct gatccgcttc
    16501 gatacgtcca acttcgggcc gtccggagcc aagggggagc gggaagccgc cgagtacgtc
    16561 gccgccaagc tggacgaggt cggcatcgtc gccgaactct tcgaatccga gccgcggcgc
    16621 accacgctgg tcgccgactg gtcgcccgag ggctgcgaca cctcgctgcc gccgctgctc
    16681 gtccacggcc acaccgacgt cgttcccgcg atcgcatccg actggcaggt ggacccgttc
    16741 gccggcgaga tcatcgacgg ctacgtgtgg ggccgcggcg cggtcgacat gaaggacttc
    16801 gacgccatca tcctcgccgt cgtccgcgac cggatgcgca ccggacgcgc cccgcggcga
    16861 cccatccgac tcgtcttcac cgccgacgaa gaggcgggcg gtccgctggg cgcgacctgg
    16921 ttcgccaaca atcatcccga gctcatcgcc gactgcaccg aggcgatcgg tgaagtcggc
    16981 ggcttctcga tcagcgtcgc cgaccagcgc ctctacctca tccagactgc ggagaagggc
    17041 ctcgcctggc tggacctgat cgccgagggc acagccggcc acggttccat gcgcagcgac
    17101 gacaacgcca tcacccgcct cgccgaggcg gtcacgaaca tcgggcagta cagctggccc
    17161 gcccgcatcc atccggcgca gcggcagttc ctggatgcgg tcgagcaggc gctcggcatc
    17221 caggtcaacg ccgacgacgc cgagaccacc ctcgcccggc tcggctccat cgcccgcatg
    17281 gtcggcgcca ccatgtcgaa caccgccaac ccgaccatgt tgaacgccgg ctacaagcac
    17341 aacgtgattc ccggccacgc caccgccggc gtcgacggtc gcttcctgcc cggctacgag
    17401 caggagttct acgacaccat cgctcgactg ctgcccgaca aggtgcgata cgaggtttcc
    17461 atctccgagg tggcggtcga gaccaccttc gacggcgacc tcgtcgacgc catgaccgcc
    17521 agcctcctta cccaggatcc gggtgcggtc gccgtcccgt tcatcatgag cggcggcact
    17581 gacgccaagg cctggtcgcg gctgggtatg cgctgcttcg ggttcgcccc gctgcggctg
    17641 ccgccggaac tcgacttcgt cgcgctcttc catggtgtgg acgagcgcgt gcccaccgag
    17701 tccctcgagt tcggctgccg cgtgctcgac gacttgctgg accgggcatg aaccccgatc
    17761 cagagcgcgc ccgctccttc ggcgcggcgg tcgacgccta cgaggtcggc cgtccggact
    17821 actcggccga gtcggttgcc tggctgctcg aaggtgcgcc cggacgtgtc ctcgacctgg
    17881 gcgccggctc cggcaagttg acccgggcag tgctcgatct cggacgcgac gtcgtggccg
    17941 tcgacccgga cgcggccatg ctggcacgca atcccgcccc ggatacccac ctgggcaccg
    18001 cggaggcgat tccgctccct gattcgagcg tcgccgcggt ggtggttggc caggcctggc
    18061 actggttcga tccgaactcc gccggtgccg agatcgcccg ggtactggtt tccggtggcc
    18121 ggctcggcct tgtctggaac acccgcgatc gcagtcaccc gttcgtggcc gcggtcgccg
    18181 aagcgatcgg cgcctcaccg gccgaggaca tggtcgatgc tgacgaggtg ctcgccctgg
    18241 ccgggttcac cccttggcag cgacggcagt ggcagcgcgt ccgctggatg agcgcgggcg
    18301 aggtgatcgc gatggccacc tcgcgcagcg cctacctgat cgcttgcccg gccgagcagc
    18361 gggcggtggt cgaccagctc accgagctgc tggtcaccca tccgcacagc cgcggacgcg
    18421 agcgcttcga gtacaccctc aacgtgactg gctatcgctg cgaccggctg cgttgaggtg
    18481 atgctgagat cccggcctgc gccgggatga cggaagcgcg ctcttgcctc agaatcgggc
    18541 actcagccat cccggcgcgc ctacgtcatt ccggcgggcc tgcgtcatcc cggcgaaggc
    18601 cgggatctct gcagtcaatt agcctccgtt cccgcgcgat tcagcttggt cgctcgccgc
    18661 gctcggcttt cgcgacgatg ccggcgattc gtcgcgtccg ggtcgcatcc tgtttggcca
    18721 gggctatcga ggccagcgcc atcttcttcg cagcgggagg aaaggcgtcc caagtggccc
    18781 gtgcggccgg gttcgcgtcc agggcagtag tcagttcgga cgccaggtca ccacccacac
    18841 accggcgccg cgatcgtgat tcgcggccag ccagttgtgc cagtcgacaa gttcgtcggg
    18901 gtggaagcgt tccgcgtccg ccatcctgcc catcgagatc tccttccggc caagtgtcaa
    18961 cgcagggtga gggtgggctc cccaagatcg cgcatcacgt tcggtccgcc ggagacgtcg
    19021 tccagtgcgg ccacgatctc ttccggtaac tccagttcct cggtcgcgag atacggctcc
    19081 aactgcgcga cggttcgtgc gcccaacagg ggcgcggtca ctcccggagc gtcccgcacc
    19141 cacagcagcg cgacctgtgc cggggtgagg tcgagacccg ccgaagccgt gcacaccgcg
    19201 tccgtgaccg agcgtgagcg ggcttcgagg tagggctcca cgaaccacgc gaaatgctcg
    19261 gacgcgcccc gcgaacctcg gggaagcccg ctgcggtact tgccggtgag caccccgcgg
    19321 ccgatcggcg accaggggaa caggcccatg ccgaacgccc ggatcgccgg gatcacctcc
    19381 acctcggccc gcctcgccag cagcgagtac tccacctggc tggatgtgag cggcgtccgg
    19441 ccgggaaacg cggcctgcca ggtggcggcc tgggcggtct gccagccaac gaagttgcac
    19501 accccggcat agcggacgtc gccggcgccc accgcggtgt cgatggcggc cagtgattcg
    19561 tccaggggag cctggcccca cgcgtgcagc tgccacaggt cgatgtgatc ggtgcccagc
    19621 cggcgtagcg atccacgcag gtcggtgagc agggcggcgc gcgaggtgtc gacccggcgc
    19681 tccccgtccc ggatgccgaa tccggccttg gtggccacca ccacctcttc gcgagccaca
    19741 tcgccggcgc gcagcatccg gccgagcatc cgttcggcgt ccccggaggc gtagcccgct
    19801 gcggtgtcca ccaggttccc gccggccgcg acgaaggctc gcagcagcgg acgggcatcg
    19861 tccagctggg tttcgcggcc ccaggtcagg gtgcccagac cgagccggga cacggccagg
    19921 ccggacgcgc cgacgagccg ctgtctcata ccgccgggat ggtattcgta agcagcagcg
    19981 cgctgatcac cagcgcgagc gccacccggt agatgatgaa cgcggtgaag tcgttgctgg
    20041 ctacgaactt cagcagccag gcgatggacg cgtacgcgac cactccggag acgatcgtgg
    20101 ccaggatggt tgccatccag cccacgccgc ccggtgcgcc gatgttgctg gcttcggtga
    20161 ccgcttggaa tgccccggac gcgatcagcg tcggaatgcc gaggaagaag ctcatccggg
    20221 tggcgctcac ccggtcgagc ccgcggaaca ggccgcccga gatggtcgcg cccgagcggg
    20281 acacgcccgg caccagcgag accgcctgga tcaggccgag gagagcgccg tcgcgccagg
    20341 tgatcgagtg ctcgtcccgc accgccttgc caagacgatc ggcgaggaac atcaccacgc
    20401 tccagcccag cagaccggct acgaccgccc agagactgcg aagcggtccc tcgatcaggt
    20461 ccttcaacag caccgcgatg gccgcggtga tgacgaagcc gacgatgatc gcccagccca
    20521 tccggtagtc gggatcctcg cgggcttccg gcttcgccag gccgcggaac cacgcggtcg
    20581 cgaaccggac gatgtcaccc cagaagtaga tgatggaggc caccatcgcg ccgatctgga
    20641 tgatcgcggt gaaagcggtg acgctcgggt cggcgatttc gaagccgagc agcttctccg
    20701 cgacggtcag gtggccggtg ctggagatcg gcaggaactc cgtgaccccc tcgatgatgc
    20761 cgaggagaat cgcgtgccac cattcgatca ttcgttggcc ttcctgccgg tgccggtgtc
    20821 aatccggcgg taagcctagc cgtaccgctg gcgttgcagc cgggtaggct gccgcccatg
    20881 cgtgcctgga agtcggttgt cgtgccccgt ctgcggggca ccggaagtgg acgcgtgcat
    20941 gcccacgaca ccctcaccgg gcaattgcag cccgttgggc ccgaggtggg cgaggcgcgg
    21001 ctgtacgcct gtggcatcac gccctacgac gccacccacc tcggccacgc cgccacctat
    21061 gtggccgtcg acctgctgca ccgcgcttgg ctggacgcgg ggctcacggt ccgctacgcg
    21121 cagaacgtca ccgatgtgga cgatccgctg ctggaacggg ccaccgcgac cggtgccgac
    21181 tgggagcagc tggccgccga tcagatcgaa ctgttccgca ccgacatggc agcgctgcgg
    21241 gtcttgccgc cggccgagct gaccggggtg gtcgagagcc ttgaccgtgt caccgatctg
    21301 ctgggcgtgc tgcgcgacga gcgggcgatc taccaggtgc ccgatccgga gtatccggac
    21361 tggtacttct cggtgaccaa gtcgtctggg ctgatggacg gtaccggcta ctcgatgctc
    21421 gaggccgaac agatcttcgc tgagcgtggt ggtgatcccg accgtccggg caagcggcat
    21481 cgcctcgact gcctggtctg gcggcaggag cgcaacggcg agccggcgtg ggattcctgg
    21541 ctcggccgcg ggcgtccggg ttggcacatc gagtgcaccg cgatcgcact gggcgcgctg
    21601 gggaccgact tcgacgtgca ggccggcgga cgtgacttgg ccttcccgca ccacccgatg
    21661 tgcgcagcgg aggctcgggt ggccaccggt cacccgttcg cgaaggtgtt cctgcacacc
    21721 ggcatggtcg gcctggacgg cgagaagatg agcaagtcgc ggggcaacct ggttttcgtc
    21781 agccggctgc tggccgaggg cgtcgatccg atggcgatcc gcctggtgct gctggcgaac
    21841 cactaccgca gcgactggga gtacacctcg gatgtgctcg cggccgccga ggcgcgcctg
    21901 gcgaagtggc ggacggcggt cgcgcgtccg ggcgctgacg ccggcccggt catcgaggcg
    21961 gtccgcaacg cgttgcgcaa cgacctgaac gctccggccg cgctggcagc catcgacgcc
    22021 tgggccgacg cacccggcga tgctgcggac gcgtccgacc tggttcgtga ggccgtcgac
    22081 gccctgctgg gcgtggccct gtagctcacc cgctgagccg cgccgtcacc gtccgtccga
    22141 tcaccggtcc gcccgattcc ctgcaagctg gacggttggg tcccgtgacg ggatcgaagt
    22201 gtccagcttg cggaaaccgc actgcacacg cccaggttcg ccggtccgcc cgattccctg
    22261 caagctggac ggttgggtcc cgtgacggga tcgaagtgtc cagtttgcgg gaaccgcact
    22321 gcacggccag gttcgccggg gtcggcgggt cgccggtagg ctgcgcggcg tgagcaagtc
    22381 cttcgaagat ctctatgccg aactgcgcga gcgcgctgcc acccgtccgg agggttcggg
    22441 caccgtccgc gaacttgacg cgggtgtgca tgcgatcggc aagaagatcg tcgaagaagc
    22501 ggccgaggtc tggatggccg ccgagtacca gggcaaggcc gaggccgcag aggagatcag
    22561 ccagttgctg tatcacctgc aggtgctgat ggtctcgctg ggcctggaac tcgaagacgt
    22621 ctacgaatac ctgtgaggag ttcccggtga ccgggttgtt gaagatcgcc gtcccgaaca
    22681 agggttcact cgctgagggc gccgccggca tgctacgcga agccgggtac cgccagcgtg
    22741 ccgacgccaa ggagttggtg ctcgtcgacg atgccaacgg tgtcgagttc tactacctgc
    22801 ggcccaagga catcgccgtg tacgtggggg agggcaccct cgacctcggc atcaccggac
    22861 gcgacatgct gctcgactcc ggcgccgaag ccgaagaggt gaagtcgctg ggcttcggcg
    22921 gtacccggtt ccggttcgcc gcccccggcg gcgccacgat gagcgtcgcc gacctgaccg
    22981 gcaagcggat cgccacctcg tactcgggcc tggtcgccag ctacctcgac ggccacggcg
    23041 tgacggcccg gctgatccac ctggacggag cggtcgaaag ctcggtgcgc ctcggcgtgg
    23101 cggacgtggt ggcggacgtg gtcgagaccg gcaccaccct caagcgcgcc ggcctggagc
    23161 tgttcggcga cgcgatcctg gaatccgagg cgatcctgat ccagcgcaag ggcgtgcccg
    23221 caccggaggg cctcgacctg ctggtgcgcc ggttgagcgg cgtgatcacc gcgcgggact
    23281 acctgatggt cgactacaac gtgccccgag aactgctcga agcggccacc aaggtcacgc
    23341 ccgggttgga cgccccgacg gtctcgccgc tggcgaagga cggctgggtg gcggtgcggg
    23401 cgatgatccc ggccaagcag gcccagcgca tcatggacgc cctttgggaa gccggcgcgg
    23461 acgcgatcct ggtcaccaag atccacgcct gccggttgta ggtggctgcc gtggtgttcc
    23521 gcccgcgccg ctcactgctg atggcttcag tgctgtcggc agtgctggcg ctggccgcac
    23581 tgatcggctg ggtcacgctg ccgcccgaca tccaggtcct gttcaccgtg ccgcagctgc
    23641 tcaccctggc gttcttcgcg ctgttcatga tcggtttcat gatgagcatc gggctcagct
    23701 acctggccgt cgacgatgcg ggactgcgct tccgcaacgg cgtccgcacc caccggctgc
    23761 cgtggggcga ggttcgcggc ttccggttca ccgagcacga cccgtgggcc tacgtcctgc
    23821 tggacgaaga actgcagcga ccgctgatgg gcatccagcg cactgaccgc gagctggctg
    23881 aggccgactt cgccaagctg gtgcggtatt ggcacgaccg gacggcatcg tgagcggtct
    23941 ccgccagccc agcgacgctt ataccggcga ccgcggcgag ccggacgcga ccgtccgcgc
    24001 cagcctcgcg gccgcgcagc agagccttga ccattacctg cacgccatcg ctcagctctg
    24061 caccacgcgg gtgttgctgc cgatcgtgcc agtgcccgat ccggacggca gccgcttggc
    24121 cggtgtcggc cggatcgaac ccgagcaccc cgaacgcgaa gacccggctg aggtgcacgc
    24181 agagttggtt gcggtcaccc tcaccgctcc ggacggccgc atcggccttc ccgtcttcac
    24241 tggagcggac gccttcgcgg tctggcagcc ggacgcgcgt ccgctgccgt gccggctgga
    24301 cgaagccgca gccaacgcgc tcgaccaggg cgcgaccgcg ctgctgatcg acatcgccgg
    24361 cccgcacgcc ctggtgatcg aaggcgatct actcaccgaa ctggcccgca gccgtcgcct
    24421 ggtgcagctc gatgacggcg gctggggctg gctgttcgcc gccgccgagg accccgatta
    24481 gcctgcgtcc accccgtccg ggtaagcttg ctccgatcga ctgcaccgat tggtggcagt
    24541 ccgtgaaagt ggagtcctga actcccaccc gcttcaccgc ttggtgacgg gtcggtaccg
    24601 gtggtggctt acccatgtca cccctcctca gggtctccgc gtgcgcggag gcccttttct
    24661 ggtgtggcag gtccaccgag cagaaccagc cgtcctgagg aggacacatc agcactgaac
    24721 cgcggatcaa cgaacgcatc catgtgaacg aggtacgcct ggtcggccca gccggcgagc
    24781 aggtgggcat cgtgcgcatc gatgacgccc tgcggctcgc ccgcgaagcc gatctcgacc
    24841 tggtcgaggt ggcgccgatg gcgcgtccgc cggtggcgaa gttgatggac tacggcaagt
    24901 tcaagtacga agccgcccag aaggcccgcg agtcgcggcg caaccagacc aacactgtca
    24961 tcaaggaaat gaagctccgg cccaagatcg acagccacga ctacgagacc aagaagggtc
    25021 acgtcgtccg cttcctcaaa gccggcgaca aggtgaagat caccatcatg ttccgtggcc
    25081 gtgagcagag ccgtcccgag ctcggcttca acctgctgaa gaagctggcc gatgacatcg
    25141 tcgaggacgg cttcatcgag tccgcgccca agcaggacgg ccggaacatg atcatggtgc
    25201 tgtcgccgac ccgcaagaag accgaggcca aggttgaggt cgaagccgcg aaggcggcca
    25261 aggctgccga ccgggccgcc aagaccgagg ccgaacggca gcaggaggcc gagattcgcg
    25321 ccgcccacgc cgcagctgac accaagccga agaagaagcg ggggccagcc gacaacatgg
    25381 accccgacat cgacctgtaa agacctgaaa gaagagaaga gaaggactca gcagatgccg
    25441 aagatgaaga cccattcggg tgccaagaag cgctttcgcc tcaccggcac gggcaaggtc
    25501 atgcaccgcc aggccggcaa gatgcacctg aacgagcaca agccgaccag ccagacgcga
    25561 cgcctcgacg gcgacaaggt cgtggccaag ggcgacgcca agaaggcacg tcgcctgctt
    25621 ggcagctaca aggccaagtg acgcacccac ccacgatcga acaacacagg agtaactgat
    25681 atggcacgcg tgaagcgttc cgtcaacgcg cagaagaagc gtcgcgaggt tctggaccaa
    25741 gcctcgggct accggggcca gcgttcccgg ctgtaccgca aggccaagga acagctgctg
    25801 cattcctaca cctacgccta ccgcgatcgt cgcgcccgca agggtgactt ccgcaagctc
    25861 tggatccagc ggatcaacgc tgcttgccgc gcggagggca tgacctacaa ccgtttcatc
    25921 aacggtctga agaacgctgg cgtcgaggtc gatcgcaaga tcctggccga gctcgcagtg
    25981 aacgactcgg tggcctttgc cgcgctcgtg caggtggcca aggagaacca gccggctcag
    26041 gttgcctgag cgcaaccgcc ggccatgagc gtcgcttcga tcgagctacc cgagccgtcg
    26101 caggctgtcc tgcgcgcggc tcgggcgctg caacgccgcc aggagcggaa agagactggc
    26161 aagttcctgg ttgagggccg ccaggccgtc cgcgaggcac tgcgcctgcc tggggtggtg
    26221 gacgcggtct acttccgctg ggacgcggcc atcgacaact ccgacctgct cgaccaggcg
    26281 aaggctgcgg gggtcgccta ctacggcgtt tccgagcaga acctggcgac gatgaccgac
    26341 acggttacgc cgcagggcgt ggtggcggtg gccaatgccg tggacgtgcc gctcaaggcc
    26401 gtcgtcggca agaagcccaa ggactcggac aagccgaaga agaagcaccg ccgcaaggac
    26461 gtctcgctgg tggtgatctg cgcccaggtg cgcgatcccg gaaatgccgg cacggtcatc
    26521 cggtgcgcgg acgcgttcgg tgcggacgcg gtcatcctga gctcggactc ggtcgaggtg
    26581 tacaacccga aggtcgtccg ggccagcgtg gggagcatct tccacctgcc gatcgtcgtc
    26641 ggggtcgatc ttggcgaggc catcgaggcc tgccgcaagg ccggcatgca gatcttcgcc
    26701 acggacgggg ccgccggcac cgatctgacc gatctggacg ccgacttgga gaagcccacc
    26761 gcctgggtga tgggcaacga gtcctggggc ctgccggtgg aacacctggc gctggccgac
    26821 cacaccgtgg ccgtgccgat ctacggccag gccgagagct tgaacctggc caccgcggcg
    26881 gcggtgtgcc tgtacgccag cgccagcgcg cagcgtgcgt ccaactgaat ttgaggagca
    26941 ctcaacgcat gtccggtgag caggccgaac tgagccagtc cgcaatcgac gagatggtcg
    27001 ccggagctct ggcagccttc gccgccgcgg gctcgagcgc cgaactgaag gccgtccggc
    27061 tcgcgcacac cggcgacaag gctccgctcg cgctggcgaa ccgggcgatc ggctcgatcc
    27121 ccggtccgga gcgcaaggag gccgggcagc gtcttggtgt ggcccgcggc caggtggccg
    27181 cggcgctcgc cgcccgtgaa gccgagatcg gcgcggctga actggagcgc cagctgatca
    27241 ctgagcgcgt cgacgtcacc ctgccggtcg agttggctcc cgagggtgcg cggcacccga
    27301 tcacggcgct gatggacctg atgggcgatg tgttcaccgc catgggctgg gagatcgccg
    27361 aaggtccgga ggccgagggg gagtggctca acttcgacgc cttgaacatc ggccccgacc
    27421 atcccgcccg gggcacgacc gacaccctgt tcgtggaccc gatcgagcag cacatcgtga
    27481 tgcggacgca cacgtcgccc gtgcagatcc gctcgatgct cgaccgtgag ctgccgatct
    27541 acgtggtctg cccgggcaag gtctaccgcg ctgacgagtt cgacgccacc cacgtcccgg
    27601 tcttccacca gatcgaggga ttgtgcgttg acaaggggat ctcccttgcg cacctgaagg
    27661 ggacgctgga tcacctggcc cgctcgatgt tcggcgacga ggtcgagacc cggatgcgtc
    27721 cgcactattt cccgttcact gagccgtccg ctgaggtcga cctgaagtgc ttcgtctgca
    27781 agggcgagtc ggtgggcaac ccggatgcgc cgtgccgcac ctgtaagtcg cagggctgga
    27841 tcgagtgggg cggctgcggc gtcgtgaacc cgcgggtgct ggcagcctgc ggcatcgaca
    27901 ccgaggtgta ctccggcttc gcgttcggca tgggtatcga acgcacgctg atgttccgca
    27961 acaacgccgc cgacatgcgc gacatggtcg agggagatgt gcggttcagc cgctcactga
    28021 agggaatggc acgatgaagg cgccgctgag ttggctggcc gagcacgtcg agttgcccgc
    28081 gggcatcacc ggacgcgaac tcggcgaggt gctgattcgc gccggcctcg aagtcgagaa
    28141 ggtggagacc gtcggcgccg agatcaccgg tccggtggtc gtcggccgcg tcctggccac
    28201 tgtggacgag ccgcagaaga acggcaaggt catccgctgg gtgcaggtgg acgtcggtcc
    28261 cgagcacaac gagccgcacc cggactatcc gcagggttgc cgcagcgtgg tctgcggcgc
    28321 gctcaacttc gctgtgggcg acctggtggt cgtttcgctg cccggtgcag tgctggccgg
    28381 agggttcgcc atcgccgccc gcaagaccta cgggcacgtt tcggacggca tgatctgcgc
    28441 cgaggacgaa ctgggcatcg gccacgacca caccggcatc atcgtgctgc ctgccgtcga
    28501 cgactccggt cgcgcgttgc agccaggtga cgatccggcc ggcgtgctgc acctgcgcga
    28561 cgacgtgctc gacatcgacg tcagccccga catcggctac tgcctttcga tccgtggtct
    28621 ggccagagag tccgcgcaga acctgggggt cgcgttcacc gatgtgatca accgaccgac
    28681 gccggctgcc aaggcggacg gctaccaggt gcggctagac gatccggcct gtccgctgtt
    28741 cgtggcgctg agcgtggccg gcatcgaccc gtcccggccg accccgcgct ggatggctcg
    28801 ccggctcgtg atggccggca tgcgctcgat ctcactcccg gtcgacatca ccaactacgt
    28861 gatgcttgag accggccagc cgctgcacgc ctacgacgct tccctgctgc gcgggccgat
    28921 cgtggtacgc aaggccgcgg cgggcgagac gctggtcacc ctcgacgacg tcaccagaac
    28981 gctcgacccc gatgatctgc tgatcaccga cgcttccggt ccgatcggcc tcgctggcgt
    29041 gatgggcggt cagtcgaccg aactgcgcga caccaccacc gatgtggtgg tcgaggctgc
    29101 gaacttcgaa gccgtcacga tctcgcacac gctgcgtcgg cacaacctgc cttcggaggc
    29161 atccaagcgc ttccaccgcg gcgtcgaccc gaacgctgcc tacagcgctg cgcacctggc
    29221 tgcgtcgctg ttggtggagt tggccggcgg cacgctgtcg gacgcggaga ccgtggccgg
    29281 tgcggtgccg gcgatgccgg aacaggtgat tccgtccgat ttgccgtcga agatcctggg
    29341 cgtcgatgtc cccgccgagc gcgtggtcga gatcttgcta gcgagcggtg tcacggtcac
    29401 ggacgagccc acggtggctg cggggcagtc ccaaccgacc gtcagcccta cgtcccaacc
    29461 cactgtcatt ccggcgtccc aacccactgt catcccggcg ccccaaccca ctgtcattcc
    29521 ggcgcaggcc gggatctcag gccgggcctt gaagctaacg ccgcccacct ggcgtcccga
    29581 cctggtcgat ccgtacgact acgtcgagga ggtcggccgc aaggtcggct tcgaccagat
    29641 cccttcgatt gtgccgccgg caccggttgg acgcgggctg acccgcgacc aggcggcgcg
    29701 gcgcgccgtg ggccacgcgc tggccaccag cggcttcgtc gaggtgctga ctttcccgtt
    29761 cgggtcgagc gaagacctgg atcggctcgg cctcgccggc gccgatcccc ggcgcgctct
    29821 ggtgcggctg gcgaacccgc tcgccgagac ccagccctat ctgcggacga cgatcctgcc
    29881 gggtctgctg gccgcggtga accgcaacac ctcccgcggc aacgacgacc ttgcgctgtt
    29941 cgaggccgga cgggtgttcc gcgcagtctc cagcgacccg gcgccgatcc ccgcggtgac
    30001 cggacgtccg tccgacgccg agctggccgc catcgacgcg gtactgcccg atcagccacg
    30061 actgctggcc gctgtcctca ccgggcagtg gcggcgtgcg ggctggcagg gcgcggccgt
    30121 gcccgccggc tggacgcagg cgttcgcttt cgccgacatc gcagccaaga ccgtcggtgc
    30181 cgcggtcgag cgtcgcagcg cggagttcgc gccttggcat ccgggccgct gcgccgagtt
    30241 ggtggttgcg ggcgaggcga tcgggcacgc cggtgaactg cacccggcgg tggtcgctgc
    30301 gttcgggctg cccgcgcgca cggctgcggt cgaactcgac ctcgatgcac tggttcggct
    30361 tgcaccggtc ggcggatcga tcctgccagt ctcgggccat ccggtggcca aggaggacgt
    30421 cgccgtggtg gtgccgaacg aggtcggtgc cggtgaggtg caacaggccc tcatcgaagg
    30481 agcgggggag ctgctggaga cgatcgcgct gttcgacgtg ttcaccggtg ggcaggttcc
    30541 agagggacac cgctcgctcg cgtttgcgct gcgcttccgt gcgtccgacc gcaccctcac
    30601 cgacgtggag accgccgcgg cgcgcgatgc ggccgttgcg ctggcagcag cccggcacgg
    30661 cgcggtactg cgctcgttct aggaatcgtg atgatcggtg gacgccacgc gttcaccgac
    30721 tcagccagcg ttccctgaac acgtcgaagg gctgctcagc gcgtttttca aatcttgctt
    30781 cctctaggac acatcgtgta gacgaactgc ttctcgtcct tcagcagact ggtgttgtca
    30841 ttgatggccg acgtaagcac cacggtcgat ggtgtcgctg agttctgcga cggctgctgg
    30901 ggtttctcac caggttccag tcgctgtact cccagtcgta gctgtgtcgt gtcccgaact
    30961 tgtcgaacac gtcggcggtg gacacgctgg cttgccagcg gaaccagaac ggagtgttgc
    31021 caagggcctt gaccgttacg gtcttgcaca ccctttgcgc gtcgacctct tcgggcgcga
    31081 tgggctcagc atctccgtcc gccattctcg cccatcagag cggggactcg tcgcagggcg
    31141 gtgccgcaat gaaagactgg cggcatgcgg aacgtgcgtg acatcgaatg ctggctcacc
    31201 gacatggacg gagtgctggt ccacgacgag ctgccggtac ccggcgcggt cgaattgctc
    31261 aatcgctggg tggacacctc ccggcgcttc ctcgtgctca ccaacaactc gacctacacc
    31321 ccgcgcgacc tggctgcgcg gttggcgcgc tcggggctgc cggtgccgga ggagaatctg
    31381 tggacctcgg cgatggccac cggcgcgttc ctggccggtc aacgcggggc caagaccgca
    31441 tttgtgatcg gcgaggcggg attgaccacg gccatgcatg aggccggttt cgtgatgacc
    31501 gaaaccgacc cggcctacgt cgtgatcggt gaaacccgga cctactcctt cgaagcgatc
    31561 accaaggcaa tccggctgat caacgatggc gcgcgcttca tcgtgaccaa ccccgacacc
    31621 acgggaccgt cgccgtcggg cctgttgccg gcgaccggtg cggtggcggc gatgatcacc
    31681 gcggcgacca accgccagcc ctacgtggtg ggaaagccga acccgatgat gttccgctca
    31741 gcgatgaacc ggattcaggc acactcggaa acgaccgcaa tgatcggcga ccggatggac
    31801 accgacatcg tcgccggtat ggaggccggc ctgctcaccg tgctcgtgct gtccggagtg
    31861 accagtgagc ccgagatcga ggtttatccg taccggcccg accaggtcta cggctctgtc
    31921 gccgacctgg ttcccctgat ctgagccagc tggaactcgc cgaggagtgg tcgcacgtct
    31981 catcactgaa ctgagaactc ctcgccataa tcagcagcct ccggcaagac cccggccgag
    32041 gttacgcggc acccctagtc gagagccgag gaaccgggtt tctggagttt aggagcagcc
    32101 ctcgaggagc tcaatttcga tcgaccaggt ttctggagct tatccacgag gtttgggccg
    32161 gtcacaagcc cagaattcgg gttggcgcga tgagcgcgcg cagccccacg ccattagcac
    32221 caaagatccg gttcaaacga gtcgccaccc atgctcggca ctacagcagg ccctagggga
    32281 cagggcttcg tgaacggcgg cggccagggc ctcggtcgta ggagcggcgg cgacagcaac
    32341 cacaaccaag cccacggcgc gggacgcggc agcggtcggc tcgccgatcg cgattacctg
    32401 cgggccgggc aagccagcgg ccttgaccgc agcgcgggcc actgagccgg cggtgaccac
    32461 gaaagcgtcg aagcgtccgg cctgccagtc agcaacgacc gctgccggca acgcctgggt
    32521 gaccgtctgg tagaccggga cagggcgaac ctgccagccc agttcagcga gaccggcggc
    32581 caccagggca acgccgccac caggatcggc gaccaggtcg ggcgacagcc caacagcacg
    32641 caaagctgcc gcggtggccg ggccgccggc cgcgacccgc gcgcccggcg gacgcagttc
    32701 tgcagccccg aacccattcg cgaccagcac attcaccgtc gtgaccgaac tgattaccaa
    32761 ccagtccaag cccgcagcga cggcaggccg aaagtcgtcc accggtaagg cctccggcag
    32821 ggtgaactgc gcctcgacca cttcagcttc ggacgcggtg agcaacttgg ccaagccacc
    32881 gggagcacgc atcggcataa ggatgcggcg cccagccaac gccgcgggtt tgctggcgcc
    32941 cagattgacc agctccgccg cgcccccggc gagcagctgt cgggccgccg ccgcgccgac
    33001 tgcctgcgct tccatggctt cagtgaccgc tgccgtggtg gtgcaggtca gtatccggct
    33061 gccgtcggta tccaccaccc gggcggccag ctgcaggcac ccgtccgtga accgggcgta
    33121 ggcagcgacc ggcgcggcgc agcccgcagc caatccggcc agcacttccc gctcggcgag
    33181 agcacgaccg cgtcgtaggc agtcgtcatc gcagcactcg tctcagctct tcggtgccga
    33241 tccgtcggct ggtgaagaaa ggcgtttcca ctcggacgtg acgccgtgct cccgtagcac
    33301 gcaggtggcg catcaggtcg accaggtcgg tgagctcttc ggcctccagg cccagcagcc
    33361 actcgtagtc acccagcgcg aacgccgcga ccgtgttgct caggacctgc gggtactcgc
    33421 gtccagcccg gccgtgctcg ccgagcagcg cgcgacgctc gtcctcgggc agcaggtacg
    33481 actcgtcgga gcggacgaac ggcatccgtg gcgcactggc tggaatactc attccggatg
    33541 gggcataatc attcgcatga ccgttagagt cgccattgcc ggagccactg gctatgccgg
    33601 aggtgagttg ctacgcctgc tgctggccca tcccgaggtg gagatcggcg cactgaccgc
    33661 cgggtcgagt gctgggacgc cgctgctggc gcaccatcag cacctgaccc cactggctga
    33721 ccgagtggtt gccgagacga ccgccgccaa tctcgccgat cacgacgtgg tgttcctggc
    33781 cttgccgcat ggcacctcgg cggagatcgc cgcccagctc ggcccggacg tgctggtggt
    33841 ggacgccggc gccgaccatc ggctgagcga tcccgagcag tggcgcaagt tctacggcag
    33901 cgagcacgcg ggcacctggc cgtatgggct gcctgagctt ccgggccagc gcgctcaact
    33961 ggcccacacc tcgcggatcg ccgtgccggg ttgctacccg accaccgtca cccttgcgct
    34021 actgccggcg ctcgtggccg ggctggtgac cggtgcggat gtggtcgcgg tggccgcgtc
    34081 cgggccgtcc ggggcgggca aggcgctgaa gccgcacctt ctgggtgccg agctgatggg
    34141 ctcggccagc gcctacggcg tcggcggcgt gcaccggcac gtgcccgaac tgctgcagaa
    34201 cttcactcgg ctcggcgcgg tcgaacccag cgtgtccttc acgccggtgc tggtaccgat
    34261 gtcgcggggc atcctcgcga ccgtgaccgc tcccttggcg cggcccgttt ccgctgttga
    34321 ggcgcgcgag gtctacgcgt ccgcctacct agcggagccg ttcgtcaccg tgctgcccga
    34381 gggtctgtgg ccgcaaaccc agtccatcgt cggctccaac gccgtgcata tccaggtggc
    34441 cattgacgcg gacgccaacc ggctggtcgc ggtggctgcg ctcgacaacc tcaccaaggg
    34501 gacggccggt ggcgccatcc aatccatgaa cctcgccctg gggctgcccg agaccctggg
    34561 cctgaccacg atcggagtcg ccccgtgagc gtaaccgcag ccaagggatt caccgcagcc
    34621 ggtgtgaccg ccggcctgaa ggcgtccggc aaacccgacc tggccctggt agtcaacgaa
    34681 ggtccggacg cgcacgctgc cgcggtgttc accagtaacc ggttcaccgc cgcaccggtg
    34741 cagtggagcc agcaggcggt cgccaacggc cgactggccg ccgtggtgct gaactccggt
    34801 ggcgccaacg cctgcaccgg gtccgccggc ctcgccgacg cccagcagat ggccgagtgg
    34861 acggctgccg aggttggctg cgatgtcgct gatgtcgcgg tgtgctcgac cgggctgatc
    34921 ggcgtccggc tgccgatgga cgcggtgctc accggcatcc agctggctgc acaggacctg
    34981 gccaacaccg gtggcccgtc cgctgctgcg gcgatcatta ccaccgacac cgtcgcgaag
    35041 gagtccatcc acacctctcc ggacggctgg acgatcggcg gcatggccaa gggcgccggc
    35101 atgctcgcgc ccgcactggc caccatgctg gtggtcatca ccaccgacgc cgtggtcgaa
    35161 ccggcgcagc tggacgaggc actacgcacc gccaccggct tcaccttcga tcgcactgat
    35221 tccgacgggt gcatgtccac caacgacacc gtgctgctgc tggcttccgg cgccgcccgg
    35281 gtgcaggccg acccggccga gttctccgaa gccctcaccg gcgtctgcgc cgatctggcc
    35341 aagcagttgc tggcggacgc ggagggctcc gcgcacgaca tcgaggtgcg ggtggagggg
    35401 gccgcgtccg aggcggacgc gctcgcggtg gccagggcga tcgcccgcag caacctgttc
    35461 aagtgcgcca tcttcggcaa cgacccgaac tgggggcgcg tgctcgcgtc cgccggtacg
    35521 gtgccggccg cctacgaccc cgagcacgtc gatgtggcgt tcaacggcgt ccgcgtgttc
    35581 gtggacggcc agctcggcgc cgaccgcagc gaagtcgacc tgaccaaggg tcgggaggtg
    35641 ctggtggagg tcaacctgca cgccggcgag gccgcggcgg ccatctggac caacgacctc
    35701 acctacgact acgtcaaaga gaacgcggag tactccacat gatcgacgcc ggcgataagg
    35761 ctgccatcct gatcgaggcg ctgccctggt tggagcagta cgtgggcaag acgatcgtgg
    35821 tcaagtacgg cggcaacgcg atggtcgacg actctttgaa ggctgcgttc gccaccgaca
    35881 tcgtcttcct gcgcatggtc ggggtgaagc cggtggtggt gcacggcggc ggaccgcaga
    35941 tctcctcgat gctgaagcgt ctgggcatcg agagtgagtt tcgcggcggg ctgcgggtga
    36001 ccacgcccga ggcgatggac gtcgtccgga tggtgctgat ggggcaggtg aaccgcgagt
    36061 tggtcggcct gctgaacgcg cacgggccgc tcgccgtcgg gctctccggg gaggacgcgg
    36121 ggctgctcac ggccgaacgc aaggggctgc acgtcgacgg cgaggagatc gacctcggcc
    36181 tggtcggcga ggtgaccgag gtgcgcccgg aggccgtggt ggacctgatc aacgccggcc
    36241 ggattcccgt ggtggccacc gtcgcgcccg atgaggacgg ccaggtgcac aacgtgaacg
    36301 ccgacaccgc cgccggagcg ctggcggccg ccctgggcgc ggagcggctg atcgtgctca
    36361 ccgacgtcga ggggctgtac cgggactggc cgaactcgac cgaggtgatc cgggagatct
    36421 cttcgaccga cctgagcgag ctgctgccaa cgctcgcttc cgggatgatc ccgaagatgg
    36481 aggcgtgtct tcaggccgtc cgcgccggcg tgcccaaggc gaccgtgatc gacggccggg
    36541 ttgcccactc gctgctgctg gagatcttca ccgacgaggg caccggcacc atggtcgtcg
    36601 accatgccca gccccggccg gggacgccgg tccggctggt ggagcctgtc gagaccacgg
    36661 gtgtgccgac ctcatgacca tcacaacact gggctcccag gccgaactgc aggcccgcta
    36721 cagcgggacg ctgatgaaca cgttcggggc acccaagcgg gttttcgtcc gcggcgaggg
    36781 cgcctacctc tgggacgccg acggcaaccg ctacctcgac ctgctggccg gccttgccgt
    36841 gaacgcgctc ggacacgccc acccacaggt gcttggctct atctcggccc agctctcgac
    36901 gctcggccac gtatccaact tctttgcgac gcccactcag atcgcgctgg ccgaacggct
    36961 ggccggtttc gtgagggcga actcgcccgg gctgccagcg cgcaccttct tcaccaactc
    37021 gggcaccgag gcgaacgaag ccggcttcaa gatcacccgt atgaccggac gcaagaagct
    37081 gatcgcaatg tccggggcgt tccacggacg cagcctcggc gcgctggcgc tgaccgcgaa
    37141 cccggcctac cgggagccgt tcgagccgct gccaggcgtt gtcgagttcg tgccctatgg
    37201 ggacgcggac gcgctggccg cagtcttgga cgagacggtg gccgcagtga tcatcgagcc
    37261 gatccagggc gagaacggag tggtcgaacc gccctcgggc tttctcgctg cggctcggga
    37321 actcaccagt gaggttgggg cgctgctgtg gttcgacgag gtgcagaccg gcatcggccg
    37381 ctgtggtgac tggctggcga gtacggcttc gggggtgact gctgacatcg tgaccttcgc
    37441 caagggcctc gggaacggct tcccgatcgg cgcgtgcgtt gccaccggcc cggcagcatc
    37501 actgctcgga ccgggctcgc acggcaccac cttcggcggc aacccggtcg cggccgcggc
    37561 cggactggcg gtgctgaaca tcatcgagcg ggacggactg ctggcgcggg cggtggctgc
    37621 cggcgagcat ctggccaccg gcgtgctcga cctcgggcac tcactggtcg ccggcgtgcg
    37681 cggacggggt ctgcttcgtg gcttggtgct ggccgaaccg gtcgccgcgg tggtggccga
    37741 gcgcgcgctg gacgccggct tcgtgatcaa cgccccgcgt ccgaacgtac tgcggctggc
    37801 gccaccgttg atcgtcaccg atgctcagct ggacactttc ctggcggcgc ttcctgacct
    37861 gctggatgcc gcaagggcgg ctgaagcgcc cagaagggtg gctgagcttc acgaagcccc
    37921 gcaggcgccc cgatgaccga gcagcgcacg ccggtcacga agaatgcccg ccagtcccgt
    37981 atcgctgagc tgattgagcg cgagaccatc cgcagccaga ccgaactggc cggccggctg
    38041 gcggccgagg gtctggcggt ctcacaaggc actctgagta aggatctgct ggaactcggc
    38101 gcggtgcgcg tccgcaccag caccggggag ctcgcctacg cactgctggc caccgaccac
    38161 ggcggcgccc agtccggcgc gctggcgcgg cttacccgac tctgcgggga gttgctgctc
    38221 tctgccgagg cgtcggccaa tctgaccgtg ctgcgcaccc cgccaggcgc cgcgcagttc
    38281 ttcgcgtccg ccatcgacaa ggtgggctgg gactccgttc tcggtacgat cgccggcgac
    38341 gacaccgtgg tcatcgtcac ccgcgcaccc gacggcggag cggcgatggc cgaagccttc
    38401 ctgagcctgc acccgagttg aggaggaccc gaatgaccga agccaccgat gccggacgac
    38461 tctggggcgg ccggttcgcc ggcgggcctg ccgatgccat gttcgcactc agccagtcca
    38521 cccagttcga ctggcgcctc gcgcgccacg acctggcggg ttccaaggct catgccaagg
    38581 ccctgcacgc agctggtctg ttgaccgaca ctgaactggg cggcatgctc actggcctcg
    38641 aagcgctcga cgccgacgtc gccagtggag ccttcacccc cgacccgacc gatgaggatg
    38701 tccacggcgc gctcgaacgt ggcctgattg ccaaggtggg cctggacctc ggcggacgcc
    38761 tgcgcgccgg ccgctcacgc aacgaccaga tcgcgacgct gatccgtgcc tacctgcggg
    38821 acgcgaacgc cgatctagcc ggcgacctgc gggccctggt gtcggcgctg gccgaccagg
    38881 ccgaagccaa cctcgccgcg atcatgcccg gccgcacgca tctgcagtcc gcgcagccga
    38941 tcctgctcag ccaccacctg ctcgcccacg cctggccctt ggttcgcgac ctgcagcggc
    39001 tggccgacct cggcaatcgg ctggcgatca gcccgtacgg ctccggcgcg ctggccggca
    39061 cctcgctcgg cctcgacccc gagctggtcg ccgccgaact gggcttcacc ggctcggcgc
    39121 ccaacagcat cgacggcacc gcggcccgtg acctggtggc tgaagaggcc tacgtcctcg
    39181 cccagatcgg ggtggacctc tcccggctca gtgaggacgt gatcctctgg tgcacccacg
    39241 agttcggctt cgccaagctc gccgacgcgt ggtcgaccgg atcgtcgatc atgccgcaga
    39301 agaagaatcc cgatgtcgcc gagctcgccc gcggtaaggc cggacgactg atcggcaacc
    39361 tcgccggcct gctcgccacc tgcaaaggcc tgccgctggc gtacaaccgc gatctgcaag
    39421 aggacaagga gccggtcttc gacggtctcg accagctgcg catcctgctg cccgcggtga
    39481 ccggcatggt ggccacgctc acgttcgacc acgcccggat ggccgagctg gccccgcgcg
    39541 gattctcgct ggccaccgac gtcgccgact ggctggttcg tcagcgggtg ccgttcgccc
    39601 gcgcccacga gatcgccggc gcctgcgtcc gctactgcga ggcacgtggg caggaactgt
    39661 ccgacctgac cgtcccccag cttgccgaga tctcgcccga gttgggcgag ggcgtgctcg
    39721 cggtgctcag tgcagccggc tcggtggcat cccggaacgg ccgcggcggc accgctcccg
    39781 accaggtgcg tagccagctc gccgaactgc gggccgcgat cgcctgaccg acccgccagg
    39841 tgagctctgg attcccggcc aacgcccgga tgacgggtag ggccgggtgg cggttgcgcg
    39901 gtgctccgag gtcccggcgt tcgccgggat gaccgggtaa gccggggtga cgggtagggc
    39961 cgggtggcgg ttgcgcggtg ctccgaggtc ccgggtacgc cgagatgacg gggtatgccg
    40021 gcatgtcgga taagccggga ttgctggact cgcgggtcgc tcaggccggc ggtgcccctt
    40081 gtcggtcgca ctggccccag atatcaggac gggttatcca ctggcaccca ccttcctcgc
    40141 tgtcgcctgg acacccgcaa gtcggtccag gtcgctaccg gcgccagacc gcccgccagc
    40201 gctttcgctg ccgcccaggc gacctgctcc tcttcggcat cgttggccgc cggcaccagg
    40261 aagcggatgg tggcgcgcgg tgggccagat ccgcgggcca gcggttccac gtccacgaac
    40321 gcgtcctcga cctgccatcc cagggccacc gcggtccggg ccgccgccag caccgccggg
    40381 ggtggcactc ctgggcgggc ggcgatgatt gctgcgctgg ctcgaaacga cggcatgttc
    40441 acctcctgcg agcaggctag gccggaccac tgacattcct gatcagccgg cccgttaggc
    40501 ttgctcgttg tgaatgcggt actggaagaa ctgacctggc gcggtttcat cgcgcactcc
    40561 accgacccgg acgcgctggg cgcggccttc gacgccggcc aggtgagttc ctatgtcggt
    40621 ttcgacccga ccgcaccgag catccacatg ggcaacctgg tgcagttgat gctggcccga
    40681 cggctgcagg ccgcgggtca ccgtcccttc ctgctggtgg gcggctcgac cggattgatc
    40741 ggtgatccga agcagagcag cgagcggaac ctcaatccac gcgagctggt tcatgagtgg
    40801 gtggagagga tcaggtccca ggtgagccga ttcgtcgact tcgacggtcc ggcggccgcc
    40861 caggtggtca acaactacga ctggaccgct gagctttcca ccctcgactt cctgcgcaac
    40921 atcggcaagc acttcagtgt gaaccgcatg cttgaccggg aagtggtcaa ggccaggctg
    40981 gaatccggca tctcctacac ggagttctcc tatgtgctcc tgcagtcgct cgactacctc
    41041 gagctgttcc gtcgctacgg aatcacgctg cagaccggcg gctccgacca atggggcaac
    41101 ctcaccgcag gagtcgacct gattcggcgg gcggagggcg cgaaagtgca cgccctggcg
    41161 acccctttgc tcaccaaggc cgacggcacc aagttcggca agaccgaggc cggcacggtc
    41221 tggctcgatc cgaccatgac cagtccctac gcgttccacc agttcttcct gaacgcggag
    41281 gacgccaagg tgatcgaata cctgaagatc ttcacggtgc gtagcgagga ggagatcgct
    41341 gagcttgcgc gccagaccgc cgagtcgccg cacctccgcg ccgcgcagcg ggcgctcgcc
    41401 gacgacatca ccgacctggt gcactcgccg acagagcggg cttcggcgac cgcagctgcc
    41461 gctgcattgt tcggacgcgg tgaactggcc gagttggacc atgccgtgct cgcggcactc
    41521 tggcacgagc tgggggagca gcaaatggcc gtcggccctg acctgccgac agtcgtggac
    41581 gtgttggccg cctccggtgt ggttgcgagc aagtccgcgg cgcgaagggc gataaccgaa
    41641 ggtggggcgt acctcaacaa ccagaagatc ggcgacccgg acgtggtggt ctcggcggcc
    41701 gatctgcttg ccggccgcta cctgatcgtg cgccgtggac ggcgcacggt tggggctgtc
    41761 gaactggttc gctgaggcag aaccggtcga cacgcccggg cgtcggggcc gatttgacgt
    41821 ggtgccgcca actgcgtaat gttcatcgag cgctgccggt gacacggacg agacagaaaa
    41881 gcttctggaa cggacgagcc gggcgccaaa ccaccactga ctagcacgga tacgttgtgc
    41941 gctcatgcca gagcccatcg aaatccgagc cgagtcgcgc ggtgggggag gcaggccagc
    42001 ccggtttgac accgagccag gagccagcta gagtaagtcg agttgcccca agcgagcgta
    42061 aagcccaaat ggatcgcaac aaagcctctc tgacaaggcc aaatggccaa gttggggaaa
    42121 cgatgagaaa cagagcggat ttgacaaagc gctccgggcc tcataaagta tgtcgagttg
    42181 ctccaagcaa tggtgaaagc caaagcgcgg aacgcacccg aaacttgaga actcaacagc
    42241 gtgctttaaa agtcaatgca caataagcac tactagatag tgcaaatcaa ccccgtctca
    42301 gggggtgcga gacgaaagtc aagcaaatcc tgaaacggat tcctttgatt attgatagat
    42361 ccagcaatgg atctgtgtca gcaatcaaac attcagttga cagcaaacag gccctcgggt
    42421 ctggctgata aatttcaacg gagagtttga tcctggctca ggacgaacgc tggcggcgtg
    42481 cttaacac
//
LOCUS       DAOPRQ010000009        42192 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_698_length_42192_cov_12.514061, whole genome shotgun sequence.
ACCESSION   DAOPRQ010000009 DAOPRQ010000000
VERSION     DAOPRQ010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 42192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 42192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42192
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_698_length_42192_cov_12.514061"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1037)
                     /locus_tag="PKJ61_01940"
     CDS             complement(<1..1037)
                     /locus_tag="PKJ61_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC12626.1"
                     /translation="MTATQPEAGASADEEAIADAAESYVSGDAKAAAALADAASAADA
                     LPMAKTTDGLALESDDGTAIEVAKDGDVTLSAPGVPEIGLSVAGDPEQTKVVGGALVQ
                     TEVAPSTDVVTRATGDGVQVIAILADENAPTEVTFPLTLPDSAKLMGQADGSISVVAD
                     VTTEIAADGEAERVAAAVDQIVDIEALASGERTTALTEEQKAALEAIPDAATTRVTET
                     VQIATIGVAWAVDANGAALDTHYEITGDRVRQVIKTNGDTAFPVIADPDWTWVLRKAL
                     ECSVGVASITLAAAPKVVAMAAKVIKLLKAAKSGSSLKKAYDAWQRLGSTDGIRLGNL
                     MSQMSTVISLV"
     gene            complement(1673..2152)
                     /locus_tag="PKJ61_01945"
     CDS             complement(1673..2152)
                     /locus_tag="PKJ61_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015582207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HOC12627.1"
                     /translation="MFTPIVGTLGYVLSADGTRVLLVHRTRDGDQHAGKWNGLGGKLE
                     PDEDVYSCLVRELREEAGIEVTSARLRGTVSWPGFGTSGEDWLGFIFVVDGYSGAVPE
                     RNDDGPLVWHEITRLDELPMWPGDRHWLPLVFGDGPAFHGVMPYVDGHPASWSYRTA"
     gene            complement(2152..3201)
                     /gene="tsaD"
                     /locus_tag="PKJ61_01950"
     CDS             complement(2152..3201)
                     /gene="tsaD"
                     /locus_tag="PKJ61_01950"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOC12628.1"
                     /translation="MTGEGPLILGIESSCDETGVGIVRGHELLANEVASSVEEHVRFG
                     GVVPEVASRAHLQAMVPTLQRACAKADIDLAEVDAIAVTAGPGLMGALVVGLAAAKSL
                     AVALNKPLYGLNHLVGHVAVDLLDHGPLPQPCAALLVSGGHTSLLAVNDITGDIVELG
                     STIDDAAGEAYDKVARLLGLAYPGGPIIDKLAAEGDPKAIAFPRGLTARKDLEKHRFD
                     YSFSGLKTAVSRWVETRRRDGLDVPVADVAASFQEAVCDVLTAKAVDACEHLGVDTIL
                     LGGGVAANSRLRGLLEERTAAAGITLRRPRPALCTDNGAMIAVLGAEVVSAGLRPSSL
                     AIGANSGLPVSTVLV"
     gene            complement(3198..3665)
                     /gene="rimI"
                     /locus_tag="PKJ61_01955"
     CDS             complement(3198..3665)
                     /gene="rimI"
                     /locus_tag="PKJ61_01955"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002520620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HOC12629.1"
                     /translation="MIITHARETDLNDIMALERAGFEPAEQWSEAAWAEELAASDRYV
                     LARLDRDARIVAVATFSCVEDMADLHRVIVHPDARGQGIASSLVRAGLEWAGAVGARR
                     MLLEVRPDNPPAVGLYRSLGFAQVSVRRDYYGAGRDAMVMLRPIGEHDEWAVA"
     gene            complement(3662..4312)
                     /gene="tsaB"
                     /locus_tag="PKJ61_01960"
     CDS             complement(3662..4312)
                     /gene="tsaB"
                     /locus_tag="PKJ61_01960"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013160553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOC12630.1"
                     /translation="MTLTLAIDTAADVRAGLARDGVPLSRAVVADRRAHAEQLLPLVQ
                     QLLAEADATPSDLTEIVVGVGPGPFTGLRVGVVAALTLGEALGVEVRGVCSLDPVAQD
                     WARQGAPADFAVVADARRKEVYWARYGADGARVAGPFVTSPDQVPAIPLAGAGAALAG
                     VCSGPAELDPALLAAFGNQLPDAGHEPLYLRRPDAEVPTKRKSTLLLPRLAKMEKQ"
     gene            complement(4366..4761)
                     /gene="tsaE"
                     /locus_tag="PKJ61_01965"
                     /pseudo
     CDS             complement(4366..4761)
                     /gene="tsaE"
                     /locus_tag="PKJ61_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004808955.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
     gene            complement(4822..5253)
                     /locus_tag="PKJ61_01970"
                     /pseudo
     CDS             complement(4822..5253)
                     /locus_tag="PKJ61_01970"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(5310..5972)
                     /locus_tag="PKJ61_01975"
     CDS             complement(5310..5972)
                     /locus_tag="PKJ61_01975"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOC12631.1"
                     /translation="MVELIILSVVVAALVIAGVWYLLVRRSANEPARRAVEPTPAPAA
                     VPEPEPEPVVVEPEPAPDPDAVAEPVAEETPIGVLSPDAVRLSGTATTKEAAIAEVGE
                     LLVKVGAVEPGYVDSMLARERSVSTYMGNLLAIPHGTNAAQNLIRRSAIAVVRYPDGI
                     DWNGNSAKFVVGIAGADDQHVRILSGIAGIFLDSASVATLTAATSVAEVMAIFAPVDE
                     GL"
     gene            complement(6055..7542)
                     /locus_tag="PKJ61_01980"
     CDS             complement(6055..7542)
                     /locus_tag="PKJ61_01980"
                     /inference="COORDINATES: protein motif:HMM:NF013425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HOC12632.1"
                     /translation="MERGYTVAQIREAEARAFEVVAPGELMQRAAAGLATAILRRLRA
                     SVIPALPTVIPTGSGPTVIPAKAGIPKPTRPIRAYGRRVLLVVGSGNNGGDALYAGVR
                     LLRRGVRVEAWRTGSAVHAAAWADYLAAGGREVHSAAALDGLGGYALVVDGVAGIGSR
                     PGLDGAVAEFAGAVARSNVPVVAVDLPSGLAPEPPFAAEPHFEAALTVTFGGLKACHL
                     MEPAQSACGEVELVDIGLELPEPAFVRWEASDLAAAWPVPDASSDKYSRGVVGFDTGS
                     PSYPGAAVLGVAGAVGAGAGMVRYLGSPEAAARVLDRFPNVVVGDGRVQALVLGSGWC
                     DRADLGEVARAVAGEVPVVLDADAIRQLSGRGRPEVVLTPHAGELAGLLGWRREQVAA
                     DPVAAVLAAVGRTNCTVLLKGATQYVATPDDDRIWVALPGVAWTAQAGSGDVLAGICG
                     TLLAAGLAPVDAALAAASVQALTASAYPGPLPPQDLAARLVVPTS"
     gene            complement(7542..7892)
                     /locus_tag="PKJ61_01985"
     CDS             complement(7542..7892)
                     /locus_tag="PKJ61_01985"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HOC12633.1"
                     /translation="MIVGIGLDLTEVFRFSRAVERPGVLERLFTTAEREDAPTERLAG
                     RFAAKEALAKSLGAPHGLVWTDVEVLRDDLGKPYFVFYGTVAERVAALGIANVHLSIT
                     HDAGVAAAMVVCES"
     gene            complement(7889..9733)
                     /gene="glmS"
                     /locus_tag="PKJ61_01990"
     CDS             complement(7889..9733)
                     /gene="glmS"
                     /locus_tag="PKJ61_01990"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004808965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HOC12634.1"
                     /translation="MCGIIGYTGPRVAREVVVSGLRRMEYRGYDSAGVAVLGDDGIEI
                     RKKAGKISNLDAELAQRPLPDSGTGIGHSRWATHGGPTDANAHPHVSANGRVALIHNG
                     IIENFAALRAELEATGVEFKSQTDTETAAQLLGLEVAGGADLADAMRAVCRRLQGAFT
                     LVAIDRDQPGRLVAARRNSPLMVGVGEGENFVASDVSAFIEFTRSAIELGQDQVVDVT
                     ADSIEVTDFNGAPAESHPFEVTWDLSAAEKAGYDWFMRKEIYEQPQAVADTLLGRFTP
                     DGALKLDELHIPEAELQLVDKIIIVACGSAYYAGLVAKYAIEHWTRVACEVEIASEFR
                     YRDPIITGSTLVVTISQSGETADTLMAIRHARAQRAKVIAICNTNGATIPRESDAVIY
                     THAGPEIGVASTKGFLTQVAACYLLGLYLAQVRGTMFDDEIRVVMSELAGTPKLIQQV
                     LDGLDSVKELAARFVDQKSVLFLGRHVGYPVALEGALKLKEIAYLHAEGFPAGELKHG
                     PIALVEDGIPIFIVVPPQGRDQLHDKVVSNIQEVRARGALTIVLAEDGDHEVEPYADV
                     LIRLPKVSTLLQPLVAMAPLQMFACELATLKGYDVDQPRNLAKSVTVE"
     gene            9813..10793
                     /gene="coaA"
                     /locus_tag="PKJ61_01995"
     CDS             9813..10793
                     /gene="coaA"
                     /locus_tag="PKJ61_01995"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002522082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HOC12635.1"
                     /translation="MSQPAVAAQPAAPEDHPYGPYLTLDRQHWAALAAARPDNLDAVT
                     LRRLRGIGDPTDAAEVREVYLPLTDLLSLYTERTGALFASSHDFLGLTGQRTPFVIGV
                     AGSVAVGKSTTSRLLKELLSRLPSKPRVDLVTTDGFLFPNAQLAELGLLERKGYPESY
                     DRHRLLRFVMDVKSGAPTVTAPVYSHLIYDIVPDQVVTVSQPDILIVEGLNVLQPARR
                     RANGSTGLAVSDFFDFSVYVDAADDDIRSWYIARFLQLWETAFRDPLSYFVRFSELSR
                     EEATAQAGSIWDAINGPNLDLNIRPTRGRATAILRKGTDHQVSWVRIRKV"
     gene            10795..11217
                     /locus_tag="PKJ61_02000"
     CDS             10795..11217
                     /locus_tag="PKJ61_02000"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF002959.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HOC12636.1"
                     /translation="MAVLPLVAADAADAVALWHDAGLTRPWNDATADFLRAVRGPSSA
                     VLGVREQEALIGAAMVGHDGHRGWVYYLAVADAARGRGLGRELMLAAEDWLVSRDIPK
                     LQFMVRTDNAAVLDFYAHLGYAQQEVLVLGRRLDTAYA"
     gene            11323..12006
                     /locus_tag="PKJ61_02005"
     CDS             11323..12006
                     /locus_tag="PKJ61_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOC12637.1"
                     /translation="MQIKEVPAASLRHPRMAIETIAGVELPEWDLVAEQMDRVEVAAG
                     ERLVTPGKSDPYVYFVTAGLVKVQLNPSPSDPAQGVTLFPMEEGDLFAAPTVLQLPGI
                     QRAVQRGLHPRSSYLAEVDRDIAMHTLIALEPSVVLRCPGSVIAELAERHQPWAQLLA
                     TIALLNNAALAQDLASQKFPAEQRYRTLATNRPDLVRRLPQREIASLLNITDSALSRI
                     AKRVRSAAE"
     gene            complement(12009..13373)
                     /gene="glmM"
                     /locus_tag="PKJ61_02010"
     CDS             complement(12009..13373)
                     /gene="glmM"
                     /locus_tag="PKJ61_02010"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002514806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOC12638.1"
                     /translation="MARLFGTDGVRGTANLELTAEIALELSVAAAHVLGEAGAFQRSR
                     RPVALVGRDTRASGEFLEAAVVAGLASAGVDVVRLGVIPTPGVAHLVGQLDADLGVML
                     SASHNPMPDNGIKFFARGGVKLDDDIEDAIEERMAEVWLRPTGAGVGRVADDPGAIES
                     YIAHLVASLGPDASLQGLKVVVDCAHGAAHVTGLAAFEAQGAEVVALHASPDGLNIND
                     ACGSTHMGALQAAVVEHGADLGIALDGDADRCLAVDATGEIVDGDQILAVLALAMREN
                     QTLHSDTVVATVMSNLGFINAMRAEGIAVDQTKVGDRYVLEAMNANGFTLGGEQSGHV
                     IMSEFATTGDGVLTALHLAARMVRTGKPLRELAAVMVRLPQVMINVPNVNKVRAGIDP
                     VVNAAVSAAARELGRAGRVLLRPSGTEPLVRVMVEAGTETQADEIARRLAGVVKERLS
                     LEPA"
     gene            complement(13387..13902)
                     /gene="rpsI"
                     /locus_tag="PKJ61_02015"
     CDS             complement(13387..13902)
                     /gene="rpsI"
                     /locus_tag="PKJ61_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016666057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOC12639.1"
                     /translation="MTETVEETTVDEVVPFVEDDREIAYRAEAVASAPLPGGRPAVVA
                     PGAATGRRKEAIARVRILPGTGQFSINGRTLEDYFPNKLHQQTVNEPFVTAAVMGSYD
                     VIATIVGGGVTGQAGALRLGIARALNAVDVEASRPALKKAGLLTRDARVKERKKAGLK
                     KARKAPQYSKR"
     gene            complement(13917..14360)
                     /gene="rplM"
                     /locus_tag="PKJ61_02020"
     CDS             complement(13917..14360)
                     /gene="rplM"
                     /locus_tag="PKJ61_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOC12640.1"
                     /translation="MSTYSPKPGEITRDWHVIDAEDIVLGRLAVTAATLLRGKHKPQF
                     APHVDTGDFVIVVNASKIALTGNKATDKMAYRHSGRPGGLKAVPYGELLASDPRKAVE
                     RAVWGMLPKNKLSRQLIKKLKVYSGPEHPHSAQKPQPYQITQIAQ"
     gene            14551..15831
                     /locus_tag="PKJ61_02025"
     CDS             14551..15831
                     /locus_tag="PKJ61_02025"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HOC12641.1"
                     /translation="MADILQIRHAASGTEAELAITDATIRAPELRQFNIGGEPLATYD
                     PGFMNTASCRSAITYIDGEAGILEYRGYPIAELAEKSTYLEVAWLLLNGELPTASQLS
                     GWEQLITEHRMLPDKLKTVLAGFPYDAHPMTILMASTAAFYSFYPDAQNFTDTEARAL
                     NIARLIGKMPTLAAFAYRKGKAKPYINPDDSYGYCHNFLQMLFKQAERRYQADERLIR
                     AVEVLFILHADHEQNCSTNAVRSVASSGNDLYTCCSAGIGALHGPLHGGANEAVLKML
                     REIGSVDRVPEFIEGVKAGKVRMMGFGHRVYKNYDPRAKIIKKSVEDVFEVTGVNPLL
                     EIALELEKYGLTDEYFVKRRLYPNVDFYSGLIYEALGFPPEMFTVLFAIPRTSGWAAQ
                     YQEFITDPENKIARPKQIYTGHRTREYVPMEARG"
     gene            15938..18016
                     /locus_tag="PKJ61_02030"
     CDS             15938..18016
                     /locus_tag="PKJ61_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013160542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-like protein EF-G2"
                     /protein_id="HOC12642.1"
                     /translation="MSSKLSAASAQVTSPDDVRNVVLIGNAGSGKTSLFEQLLKARLA
                     GYRGEKEDAERAAQLYLASMVTGDVLVNLLDAPGHPDFVGELRAGIRAADAAIFVISA
                     ADGIDGAAVTLWDECAAARLPRIIALTKLDAGHADFDSSVAEAQDKFGEGVLPTHVPT
                     LSAPGVVAGNIGLLSLEEHDYSGGEVVNRKLTADDSHVETYRGPLIEAIIQEAEDDTL
                     MDRYLSGEELDTDYLLDDLRKAVATANLYPVVPVASNTGVGVEELLRLIEHGFPTPSL
                     HPNPDATTPSGDPLPNPATDPSAPLVAQVIRTTTDPFAGRLSMVRIFSGTLTTESVVH
                     VSGHRPKPDNAHPDHDNDERVGMIQAAVGTELKPRPSAMAGEIVMVPKLTTAETGDTL
                     SAKDRPALVVPWNLPEPLLPLAIRAATRNDEDKLAGALQRLAVEDTTVRLDRGGSDQL
                     VLWTTGQAHADLLTARLVDRYGVKVEQEELKIPLRETFVAKATALGRHVKQSGGHGQY
                     AVCNIEVEPLPRGAGFEFVDKVVGGAVPRQFIPSVEKGIRNQLDKGVFSGYPMVDVRV
                     TLFDGKAHSVDSSDMAFQMAGSLAIKEAATPKSVALLEPLDLVTVTVGEEYLGAAMTD
                     LTGRRGQLLGSDSENHKAIIRALVPQTELARYAIDLRGIAHGSGTFTREFHGYELLPA
                     NLMEKYTKAN"
     gene            complement(18035..18649)
                     /locus_tag="PKJ61_02035"
     CDS             complement(18035..18649)
                     /locus_tag="PKJ61_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017934867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HOC12643.1"
                     /translation="MGQFFDPNLFISTAVTLFVIVDPPGLLPVFLGLTNSLDEKLRRR
                     AANRASAVAFGVIAGFALFGRQILDYLQVSVPAMQVSGGLLLLLVALDLLMGRTEDPV
                     SHAGVNVAIVPLGTPLMAGPGAIVATMLAVQRAPYLGGYLTVAAALVVVMALVWVFLR
                     YAGLIRSVLRESGTVLASRIAGLLLSAIAVQMVADGVFGFIDVH"
     gene            complement(18667..19077)
                     /locus_tag="PKJ61_02040"
     CDS             complement(18667..19077)
                     /locus_tag="PKJ61_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014847169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HOC12644.1"
                     /translation="MRTILNLIWLLFGGFWLAVGYFFAGILACILIVTIPFGIASFRM
                     AGYALWPFGRAVIAKPSAGIGSGIGNVIWFLIGGLWLAIGHVTTAVAQAVTIVGIPLA
                     IANLKMIPVTCFPFGKEIVDARTMPPGARPLHSL"
     gene            19164..19883
                     /gene="trmB"
                     /locus_tag="PKJ61_02045"
     CDS             19164..19883
                     /gene="trmB"
                     /locus_tag="PKJ61_02045"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HOC12645.1"
                     /translation="MPAAERIHRDVVSYVRRSARMNESQQRAWDAHHDAWVVPLSTGA
                     RSTSVAADASVDWARVFGREARLCVEIGSGTGHAITALAEAMPDANLVAFEVFAPAVA
                     STLGRLARHGVTNVRVVLADGAQALATVFASASISELWTFFPDPWHKARHHKRRLVSP
                     EFAEVVASRLTADGAWRLATDWADYAEAMREVLDAAPGLANCCDGWAPRYELRPTTKY
                     EQRGLDAGRRIYDLAYRRAGV"
     gene            19880..20701
                     /gene="truA"
                     /locus_tag="PKJ61_02050"
     CDS             19880..20701
                     /gene="truA"
                     /locus_tag="PKJ61_02050"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016455559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOC12646.1"
                     /translation="MTRYRLDLGYDGAAFHGWAGQTGLRTVQGELETWIGRVLRLPAP
                     PVLTCAGRTDAGVHARGQVAHLDLDLPEPELVAATLARRLRRALPEDVAVYRVRLAPE
                     GFDARFSAIWRRYAYRLSDIGPLDPLLRGTTAAVRAELDLTLINAGARQLLGLHDFAA
                     FCRAREGATTVRELQWLEGVRLADGRIEIGVRADAFCHSMVRSLVGALVEVGSGRRDL
                     SWLSGLLHSPDRVGDIPVMPAAGLVLEEVGYPPDDQLAARAQAARAVRTLGEECP"
     gene            20698..21297
                     /locus_tag="PKJ61_02055"
     CDS             20698..21297
                     /locus_tag="PKJ61_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015071196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOC12647.1"
                     /translation="MSHYFHTPDGPANFREVQLRFWDTDWAFTTAAGVFSADGLDLGT
                     AVLLRESTPPAHARRLLDLGCGYGVIAVGLASACPDAVVDAVDTNERALELCATNAGR
                     AGVADRVRPMLPEQADPDARYDEIWSNPPIRIGKAALHELLLTWLPRLAPGGVARLVV
                     GRNLGADSLQAWLNTQGYRCERRASAKGFRVFSVVIETQ"
     gene            21368..21694
                     /locus_tag="PKJ61_02060"
     CDS             21368..21694
                     /locus_tag="PKJ61_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC12648.1"
                     /translation="MTEENITPEPEGGFFSDLLDKAKDFGEKAKDFAEDAFDAAKDFT
                     EDAVEAVKDFAEDLPENAKKLADDTTAAVKDFAEDLPENAKKLADEAKSAVSHLVDDK
                     PADPQA"
     regulatory      21758..21893
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            21968..23491
                     /locus_tag="PKJ61_02065"
     CDS             21968..23491
                     /locus_tag="PKJ61_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC12649.1"
                     /translation="MSVVLRRALAVGTAATLSLLTLLPATPAQAAPQACDGVWVVVQP
                     TDATSATIKCATTHTDAVVATQSAGYTVAGSDSGFGFFVEQINGAPEPFNMNTGPWWT
                     LCTAAVSAEGVIGPWATSQTGATSTVPAKGSAIGWYLPGFSCPELSQVPEAESPSPTP
                     TPTPTPTPTPTPTAPATGTASVTSAAKWLNASVPKKWDGAGAALDVGLALATRECDFT
                     TTLGDIRDYLVKQADAYSKTSPYAAGKLAIFASAVGDDPADFGGVNLVKRVLDAVEAS
                     GRIGSSDFAFGHALAMIGLDRSGSAPTEKMVDYLLSQQLASGAWGFGATADPDTTALA
                     LIALSSEVITPTDATKAAVAKAVAWAATSQKAEGYWANYSPVDSTSLLASALKLHGVD
                     TAKALAWLGTQQLRNGAFANSLNGTEANQLSTATALYLLSGVTFATVSAPLDICAEDT
                     GEASESASAAAKSEQLANTGARGNALPLGLAGLGAVALGGVLLIVRRERYAARRAQR"
     gene            23488..24219
                     /locus_tag="PKJ61_02070"
     CDS             23488..24219
                     /locus_tag="PKJ61_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC12650.1"
                     /translation="MRIRAWLAGALATAIAVVSAPGVPALAAPKALPKSTTSCAGVWV
                     IVDRGNGQVATRCATKYRTGVQALTSAGFTVKQDDGFVLQIHGFPAKPDAKSFTKYWS
                     YWHVTRNADGTWGEWKYSDKGATTSRPAKDVAEGWRFGGSGKTAPAKEPPRAYLSSPT
                     PTVVGTAKVGNLLTVDTGRWTPTPDRITYRWYRSGKAIKGATKATYKIAKADRGKRLK
                     VVVTARGSGLQTVTKSSAQTAKVKN"
     gene            24216..25013
                     /locus_tag="PKJ61_02075"
     CDS             24216..25013
                     /locus_tag="PKJ61_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC12651.1"
                     /translation="MKRSLLASMFIALLAPLAMVGATPAVAESVKVCSGVWVIVDFGE
                     LGGISTQCATKFDTGTAALKSAGFTPTLENSMLVKLDGKPTKPDIYKAYWSYWQATRA
                     DDGSYSEWKYSDLGANSYHPTKGNAEGWRYQALADGKVHPDATPPSAPVATPEPTSKP
                     SETAKPSPKPTATKTVTKAPTSTSSATPSATDSPSASAAPTTPPATPSEPTGWPSAAS
                     PAPEGTVNSPIEPAPSGTPVGALVAGGLVLAGGAGVGAWWWLKGRHR"
     gene            24989..26125
                     /locus_tag="PKJ61_02080"
     CDS             24989..26125
                     /locus_tag="PKJ61_02080"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HOC12652.1"
                     /translation="MAEGTAPLTSLHPWAWWCWAIGIGLAVSGTTNPLLLALIATAMV
                     VVVMLRRSSAPWARSVRAYLLLSLFVIGMRVLFQVLIGGGTGETVLFTLPEIPLPEWA
                     AGIRLGGAVTLEGLIFTLYDALRLAVMLLCIGAANALANPRQALRSVPAALYEASVAV
                     VIALSVAPQLIESAQRVRKARWLRGDAGTGLRALGAIALPVLADAIERSMSLAAGMES
                     RGFARTQGLPVKRTLPVMLLSVMVATLGAFLLLSTTLWELAVTLLIVGLVGTGFGLRA
                     AGTRLQVTRYRPHPWGWRATGVAASGLLAAAVVMGTGWLDPTGGGLWPSTDPLEWPEL
                     TAPMLLAIAFVLAPLPLTIPRRFAAVSSVRTDVRVRGLRAVSRS"
     gene            26122..27717
                     /locus_tag="PKJ61_02085"
     CDS             26122..27717
                     /locus_tag="PKJ61_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOC12653.1"
                     /translation="MITFENVSVTYAGAARPTLRDVSFTLPEGDLCLVVGTTGSGKST
                     LLGAMNGLVPHFTGGTLQGRVVVGGRDTARYRPRDLADVVGYVGQDPLRGFVTDTVED
                     EIAYGMEQLGVDPVAMRKRVEETLDVMGIADLRRRPLIELSGGQQQRVAIASVLAAHP
                     KVLVLDEPTSALDPTAAQDVLSAISTLVHEVGLTVVLAEHRLERVMHAADSMIWLPGD
                     GTAQLGSPAAVLSSADVTPPLAALARTLSWAEVPLSVREARRKVQALGLSLAAPATEP
                     AAASDDQPALTARDVEVRYGEISAVRGVDLDLHGGDVTALLGRNGSGKSSLLWALQGA
                     LSCAGEIRVGAADPRKLSAADARRVVTLVPQTAADLLYLPSVAAECEQADRESSAEAG
                     TARAILRRLGADVADDHDPRDLSEGQRLALVLAIQLTARPQVLLLDEPTRGLDYQSKA
                     QLHGIIDALAASGVAVLISTHDVEFAALASRKLLLMADGEVIASGSTSDLLTSSPAYA
                     PQMAKVFAPVPVLTPQDVAKGLA"
     gene            27867..28607
                     /locus_tag="PKJ61_02090"
     CDS             27867..28607
                     /locus_tag="PKJ61_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002532714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOC12654.1"
                     /translation="MVVGAVAFSWPLFLSHTSAMSAAQAPIVFAVVLPLVLAVVLTEL
                     TGGRMDAKALAMLGVLAAVGTILRPLSAGTAGFELIFFLMILAGRVFGPGFGFALGSI
                     TLFTSALITSGVGPWLPFQMLSAGFVGLFAGLLPKASGRIEIGLLAAYGMVSAFAYGW
                     LMDFSFWPFAIGDGTQLSFNPDASPLENLHRFVLFNLATSMGWNLGRALTNAVLITLL
                     GVPLLRLLRRAARQANFVPVSLGSTLAE"
     gene            28664..29272
                     /locus_tag="PKJ61_02095"
     CDS             28664..29272
                     /locus_tag="PKJ61_02095"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002516078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HOC12655.1"
                     /translation="MAYTLPDLDYDYGALAPHISADIMELHHSKHHAAYVAGANTALE
                     KLAEAREKSDFASVPKLEKDLAFHLGGHINHSVFWKNMSPDGGGEPTGEVASAIDEFF
                     GGFEGFKGQFEAAANSLQGSGWAMLVWDTLGQRLNINQLYDQQNNLPVGQLPVLQLDM
                     WEHAFYLQYKNVKADYVKAWWNVVNWTDVADRLAKSRAVVIF"
     gene            29383..30468
                     /locus_tag="PKJ61_02100"
     CDS             29383..30468
                     /locus_tag="PKJ61_02100"
                     /inference="COORDINATES: protein motif:HMM:NF018447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase family protein"
                     /protein_id="HOC12656.1"
                     /translation="MNKAITAAALSATLLIGGATIATGLHKDVRVSVDGESLPGGAFA
                     LTVGDLLAAKGITLTAGDEVSPSVDTAISDGDAVDVRFNKPVTLTVDGTERRFFTTAT
                     TLDAALTSAALQGELTGSEMSALPTAPVPRDGLEVTVSTPKQVTLEVRGKETELTTTA
                     ASVAELLAERNLMADADDKLSPAGDAAITDGQKITLDRVEVSTKVKTEPVKFDTVEKK
                     DSSLWKGESRIVTAGKVGLAKRTYAITEVNGEVTEKKVVNEDVLTKPVDQVRKVGTKV
                     SANGVGINLARAAMWDRIARCESGNNWSINTGNGYYGGLQFNRASWNANGGRDFAALP
                     HQASRAEQITVANRYYAKAGTRPWSCA"
     gene            30663..31457
                     /locus_tag="PKJ61_02105"
     CDS             30663..31457
                     /locus_tag="PKJ61_02105"
                     /inference="COORDINATES: protein motif:HMM:NF019142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase family protein"
                     /protein_id="HOC12657.1"
                     /translation="MKIIATALLTLALLAGGAPTVEAVAAPAEPAASASQAPAVSVPA
                     KPKKVTIKDGAEKKRKVETTTATVGDLLTELGITLGEHDLVKPAVTKKLKRHTKVRIY
                     RITVSTSVVTEAVPFKVEKVKNPELARGVKNVLVAGVPGSAERTYQVTTVNGEVLKKK
                     QLLAETVLLAPVTQVVEVGTKGKALNLARLKLWNKIAKCESGGRWNIKTGNGYYGGLQ
                     FNLATWRSVGGRDFASHPHKASKAEQITVANRLYAKRGSRPWGGCA"
     gene            31554..32135
                     /gene="dcd"
                     /locus_tag="PKJ61_02110"
     CDS             31554..32135
                     /gene="dcd"
                     /locus_tag="PKJ61_02110"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016455117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HOC12658.1"
                     /translation="MLLSDRDIRAQIDAGRVVLDPWDAAMVQPSSVDVRLDRNFGIFE
                     NHRYLFIDPAADQPDLTREVATSGDEPFVLHPGEFVLGATYEHITLPDDVAARLEGKS
                     SLGRLGLLTHSTAGFIDPGFSGHVTLELSNVATLPIKLYPGMKIGQLCFFQLSSPAEH
                     PYGSAAAGSHYQGQRGPTPSRSHLGFSRTQLPS"
     gene            complement(32348..32545)
                     /gene="rplD"
                     /locus_tag="PKJ61_02115"
                     /pseudo
     CDS             complement(32348..32545)
                     /gene="rplD"
                     /locus_tag="PKJ61_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015071248.1"
                     /note="L4 is important during the early stages of 50S
                     assembly; it initially binds near the 5' end of the 23S
                     rRNA; incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
     gene            complement(32672..33130)
                     /gene="rplQ"
                     /locus_tag="PKJ61_02120"
                     /pseudo
     CDS             complement(32672..33130)
                     /gene="rplQ"
                     /locus_tag="PKJ61_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004809007.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
     gene            complement(33159..34163)
                     /locus_tag="PKJ61_02125"
     CDS             complement(33159..34163)
                     /locus_tag="PKJ61_02125"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HOC12659.1"
                     /translation="MLIAQRPILTEEVVDEFRSRFVIEPLEPGFGYTLGNSMRRTLLS
                     SIPGAAVTSIKIEGNQHEFSTIPGVVEDVTEIILNLKSLVLSSEEDEPVVMYLRKAGA
                     GAVTAADIAPPAGVEVHNPELHIATLNDSGKIEMELVVERGRGYVSAVQNKNPDAEIG
                     RIPVDSVYSPVLKVTYRVEATRVEQRTDFDKLIVDVETKASMKPRDAMASAGKTLVEL
                     FGLARELNVDAEGIEIGPSPVDEQLAADLALPVEELKLTMRSYNCLKREGIHTVSELV
                     SRSEQDLLDIRNFGSKSIEEVKLRLHEMGLSLKDSSPGFDPLSALSNYDTDVDYDDSY
                     "
     gene            complement(34288..34893)
                     /gene="rpsD"
                     /locus_tag="PKJ61_02130"
     CDS             complement(34288..34893)
                     /gene="rpsD"
                     /locus_tag="PKJ61_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016666076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOC12660.1"
                     /translation="MARYTGPITKKSRRLGTDLVGNDKAFEHRPYPPGMHGRGRTKES
                     EYLLQLREKQKARFAYGVLEKQFRRYYEEAQRLSGKTGDNLLIILESRLDNVIYRAGL
                     ATTRRQARQLVVHGHFTVNGKKVNVPSYRVSAYDVIDVKEKSLNLYPLVVARETHNDK
                     VVPGWLTVRPNKGRILVHQLPVREQIVIDVSEQLIVELYSK"
     gene            complement(34920..35336)
                     /gene="rpsK"
                     /locus_tag="PKJ61_02135"
     CDS             complement(34920..35336)
                     /gene="rpsK"
                     /locus_tag="PKJ61_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015071209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOC12661.1"
                     /translation="MATAGRTASQKAAKSKLRRKEKKNVVAGQAHIKSTFNNTIISIT
                     DPTGAVISWASAGTVGFKGSRKSTPFAAQMAAEAAGRRAMEHGMKRVDVFVKGPGSGR
                     ETAIRSLGAVGLEIGPISDVTPVPHNGCRPPKRRRV"
     gene            complement(35373..35747)
                     /gene="rpsM"
                     /locus_tag="PKJ61_02140"
     CDS             complement(35373..35747)
                     /gene="rpsM"
                     /locus_tag="PKJ61_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002549911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOC12662.1"
                     /translation="MARLIGVDLPREKRLEIALTYIYGIGRTRAKETLAATGISGDIR
                     VHQLTDDQLVALRDHLEANYETEGDLRRSVAADIRRKIEIGSYQGRRHRMGLPVRGQR
                     TRTNARTRKGKKKAVAGKKKAR"
     gene            complement(35951..36064)
                     /gene="rpmJ"
                     /locus_tag="PKJ61_02145"
     CDS             complement(35951..36064)
                     /gene="rpmJ"
                     /locus_tag="PKJ61_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HOC12663.1"
                     /translation="MKVQPSVKKICDKCKVIRRHGRVMVICENPRHKQRQG"
     gene            complement(36161..36382)
                     /gene="infA"
                     /locus_tag="PKJ61_02150"
     CDS             complement(36161..36382)
                     /gene="infA"
                     /locus_tag="PKJ61_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOC12664.1"
                     /translation="MAKKEGALELEGTVVEALPNAMFRVELANGHRVLATISGKMRQH
                     YIRIIPADRVVVELSPYDLSRGRIVFRHR"
     gene            36650..37009
                     /locus_tag="PKJ61_02155"
     CDS             36650..37009
                     /locus_tag="PKJ61_02155"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOC12665.1"
                     /translation="MAIDKQLLKGTIPLLVLHLISQGEMYGYQLIRSLNDASAGEFKF
                     SEGTLYPVLHALERDGQLRAYWRVADTGRKRKYYAITAAGVRSLSRRTAEWRTFAGAI
                     EAVLSEEIPHDATNRRN"
     gene            37012..37407
                     /locus_tag="PKJ61_02160"
     CDS             37012..37407
                     /locus_tag="PKJ61_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC12666.1"
                     /translation="MNRVLSSWPYVLFVALCWLSWELIGVLEFPGMDSAQQKSLFLTI
                     SLFVYPVLLFLAGTALGYRRGYDWVTVLACLVVFIVCQVFSSAVKAYPLSIGDLATFS
                     AYYLIPAHLGIAAGLGIRAVQRRNAQPKE"
     gene            37410..37739
                     /locus_tag="PKJ61_02165"
     CDS             37410..37739
                     /locus_tag="PKJ61_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC12667.1"
                     /translation="MPEVPELHAFASRATAGIAAPAERAGATSELYEHALSAYEEHRA
                     SGLAHDDAVQAALTQLGEPSEYRKDFARAHRARLTPRTVLLIGGAAIVFMILLWAFFA
                     LIFGVAS"
     gene            37845..40028
                     /locus_tag="PKJ61_02170"
     CDS             37845..40028
                     /locus_tag="PKJ61_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOC12668.1"
                     /translation="MDSVGTPPTTLERELAVEQAHVDLVYRHLAKAAKSARKVASQGR
                     KIYRSDRETWVREEDGTALFERDAFAFQAARRLAVLDAEHEGLVFGRLDHNDGEIRYI
                     GRIGVRDDEYEPLVIDWRARAAEPFYRATATDPMDVVRRRVLRCRNDIVVGIEDDLLD
                     SSHSHDDLVIVGEGALMAALKRARGHKMRDIVATIQAEQDEAIRADYPGVTVISGGPG
                     TGKTVVALHRAAYLLYSNRRRFERGGILVIGPSSLFMNYIERVLPSLGEDSVTLRPIG
                     GVAVDTLGFTAARIDSAPAATVKGSLRMVSLLKRLVNLPWLDPMHEQAPLRVTVKGEV
                     LSLSETSLSRIRRDVLTRTKANAGRAQAEAAVMDALYAQLPAELEMDQDTMTELVSDS
                     AAYRMFLQAWWPDLTATDVLRRLASERIVGRVSEGILDAVEQAELAASFSFEDWSVAD
                     VALLDELVAMLGPVAHDETEEPLVFLPDGTEINEVVTTADRLRDHREVDPHADPHDTF
                     AHVLVDEAQDISPMQWRMLRRRGSQASWTIVGDPAQSSWPDLAESDRALTELIGSAPH
                     RNFRLSTNYRSPAEVFRLAADVVRRDFPGADLPDAVRSTGIEPLLLAAGPDELAARLD
                     AVVAELAGQVQGTIGVIAPPSRLAGLAAAELPSVAAQGSRVSFISPLTAKGLEYDAVV
                     VVGPDEIVAESPGGVRVLYVSLTRPTQRLVTIDVGQSGAWRRDLA"
     gene            complement(39999..40904)
                     /gene="metF"
                     /locus_tag="PKJ61_02175"
     CDS             complement(39999..40904)
                     /gene="metF"
                     /locus_tag="PKJ61_02175"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HOC12669.1"
                     /translation="MPSSRPQSIAEILAAGDHPTMSFEFFPPKDDAGQDQLLDAISAL
                     EPLRPDFVSVTYGASGSTRARTIDATRLIQSTTGALTMGHLTCVSQSVAELHGAIDAY
                     TDAGVRHILAVRGDPTGGPTAAWERHPDGLNNATELVRLVKAAGDFCVGVAAFPDAHP
                     ENHDFELDARVLAEKEQAGAEFAITQLFFDADAYFRLVDRVRALGCQLPIIAGIMPVT
                     NVRQIERFATLSGADLPQPLVARLHAVADDPAAVRAVGLEIATELCDRLLSGGAPGLQ
                     FFTQNRSSATAEILARLREIAPPGT"
     gene            40956..42059
                     /locus_tag="PKJ61_02180"
     CDS             40956..42059
                     /locus_tag="PKJ61_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOC12670.1"
                     /translation="MVNLDAADPLSAELSAAIGDELAGFIDSRADALSALQPEIGELV
                     ELARAFTTGGKRLRPAFCCWGYLAAVGGDTVPDYVIRAAASLDLLHVSALVHDDVMDG
                     SATRRGLPAAHVQLAARHAASARRGAPEDFGRAGAILLGDLLLMWSVELFTSCGAPPE
                     LLASAQPLMAAVRAEVTGGQYLDISAQTRSPLDARRSPEAVLAEVRRVVEFKTAKYTV
                     VRPLQVGAALGGGSAALLDALASYGSPLGRAFQYRDDVLGVFGDAALTGKPAGDDLRE
                     GKLTVLIANAMASASSGAAEELSGLLGRELPAAEVDAARELITESGALAATEREIADA
                     TRAAVDALADGPIAAPAAEGLRKLAELATQRKS"
BASE COUNT         6804 a        14562 c        14100 g         6726 t
ORIGIN      
        1 ttgacgagcg atatcaccgt tgacatctgt gacatcaagt taccgagcct aatcccatcg
       61 gtgctgccga gcctctgcca agcgtcatag gccttcttca gcgagctacc cgactttgcg
      121 gccttgagca gcttgatcac tttcgccgcc atggcgacga ccttgggtgc agcagcgaga
      181 gtaatggacg ccaccccaac ggagcactcc agcgccttgc gaagaaccca agtccagtct
      241 gggtcagcta tcaccggaaa agcggtgtcg ccgttggtct tgatgacctg acgaacacga
      301 tcaccagtga tctcatagtg cgtatccagg gcagcgccgt tggcgtccac ggcccacgcg
      361 accccaattg tggcgatctg gactgtctcg gtgacccggg tcgttgcagc atcagggatc
      421 gcttcgagtg ccgccttctg ctcctcggtg agagcggtgg ttctttctcc acttgcaagc
      481 gcctcaatgt caacgatctg gtcaaccgcc gcggcaactc gctccgcctc accatctgcc
      541 gcgatctcgg tcgtcacatc cgcaactact gatatcgacc cgtcggcttg ccccatcaac
      601 ttcgcgctgt ctggaagggt gagcgggaac gtgacctccg tcggagcatt ctcgtcagcg
      661 aggatcgcga tcacctgcac gccatcacca gtggcccggg tcaccacgtc ggtgctgggt
      721 gccacttcgg tttggacgag cgcgccgccg acgaccttgg tctgctcggg atctccggca
      781 acgctcaacc cgatttctgg cacacctggc gccgacagcg tgacatctcc gtccttggct
      841 acctcaatgg ccgttccatc atcgctctcc agggctagtc cgtccgtggt cttcgccatg
      901 ggaagggcgt ccgctgccga ggccgcgtcc gcaagcgcag ccgcagcctt ggcatcgcct
      961 gacacgtagc tctccgccgc gtcggcgatc gcctcttcgt ccgcagaggc gcccgcctcg
     1021 ggctgggttg ccgtcaccac ctgcgccgca ggcaccgttg gggcgcttgt gtcggcatgc
     1081 gctggctgca actgcgaacc agacaccaca agtgcgaccg ataggatcgc cgccagtctt
     1141 cgccaaggct agccagcggg tagagacaag tcctccataa attcctcctt ctggggcggg
     1201 caggctggcg agaacttagc ggcaatgaat cttgcggtca accaagccca tctttgctcc
     1261 ggtcaactcc tacgcagctc cagttgatca agcgcaacga ctagaggatt gcccttgcga
     1321 agacgcgacg tgagtgaggg ccttcgctca atcgcggcgg acctcatcgc actatcaagg
     1381 aattctctat ttcctcccag cgcagagatc gcttgaccct gtggccaagc gcagggccca
     1441 ctagagaggc agctgggcgc aaccggacat ttcatcgatc ttgcgactcc gacctcggtt
     1501 caagcacggc atcccggcgt ctcggccgat ttcgcccagg aaatgtacga acttgcggac
     1561 gcagttctgg gctgccctga gaatgaagat ggtctcgaca agctcgacca gccggatcag
     1621 gggtctcgac aagttgacca gccggatcgg tggccagcat cggagggctt gatcaggcgg
     1681 tgcggtagct ccagctcgcg gggtggccgt ccacgtaggg catcacgccg tggaacgccg
     1741 gtccgtcgcc gaagaccagc gggagccagt ggcgatcgcc gggccacatc ggcagctcgt
     1801 ccagccgcgt gatctcgtgc cagaccaagg gtccgtcgtc gtttcgctcc ggcaccgcgc
     1861 cgctatagcc gtccaccacg aagatgaagc cgagccagtc ctcaccgctg gtgccgaagc
     1921 cgggccacga caccgtcccg cgcagtcggg ccgaggtcac ctcgatgccg gcctcctcgc
     1981 gcagttcgcg aaccaggcag gagtacacgt cctcgtccgg ttcgagcttg ccgcccaatc
     2041 cgttccattt tccggcgtgc tggtcgccgt cgcgggtgcg gtgcaccagc agcacgcgcg
     2101 tgccgtccgc ggagagcacg tagccgagcg tgccgacgat cggggtgaac atcagaccag
     2161 gacggtgctc accggcagcc cggagttggc cccgatcgcc agcgacgacg gacgcagccc
     2221 cgcgctcacc acctcagcgc cgagcaccgc gatcatcgcg ccgttatcgg tgcacaaggc
     2281 cggacgcggc cgccgcagcg tgatgcccgc ggctgccgtc cgctcctcca gcaggcctcg
     2341 cagccgcgag ttcgcggcca ctcccccacc gagcaggatc gtgtccacgc ccaggtgctc
     2401 acaggcgtcc acggccttgg cggtgagcac gtcgcagacc gcctcctgga aggacgcggc
     2461 cacatcggcc accggcacgt cgagcccgtc ccggcgccgg gtctcgaccc agcgcgacac
     2521 cgccgtcttc aaccccgaga acgagtagtc gaagcggtgc ttctccagat ccttgcgggc
     2581 ggtcaggccg cgcgggaaag cgatcgcctt cgggtcgccc tcggcagcca acttgtcgat
     2641 gatcggccca cccgggtagg ccagcccgag cagccgggcc accttgtcgt acgcctcgcc
     2701 ggccgcatcg tcgatggtcg aaccgagttc gacaatgtcg ccggtgatgt cgttcaccgc
     2761 cagcagggac gtgtgcccgc ccgagaccag cagcgccgcg cacggctgcg gcagcgggcc
     2821 atggtccagc aggtcgacgg ccacgtggcc caccagatgg ttcagcccgt agagcggctt
     2881 gttcagcgcc accgccagcg acttggccgc ggccaggccg accaccagcg cgcccatcag
     2941 accagggccc gcggtgaccg cgatcgcgtc cacttcggcc aggtcgatgt ccgccttcgc
     3001 gcacgcgcgc tgcagagtcg gcaccatcgc ctgcaggtgc gcgcggctgg ccacctcggg
     3061 caccactcca ccgaagcgga cgtgttcctc gaccgaggac gccacctcgt tcgccagcag
     3121 ctcgtgcccg cggacgatgc cgacgccggt ctcgtcgcag gaagactcga tgcccaggat
     3181 cagcggacct tcgccggtca tgccaccgcc cactcgtcgt gctcaccgat cggccgcagc
     3241 atgaccatgg cgtctcgacc ggcaccgtag tagtcgcggc ggacactcac ctgcgcgaac
     3301 ccgaggctgc ggtaaagccc gaccgccggc gggttgtccg gtcgcacctc cagcagcatc
     3361 cgccgggcac cgaccgcgcc cgcccactcc aaaccggccc tgaccaggga agatgcgatg
     3421 ccctgcccac gcgcgtccgg atgcacgatc acgcggtgca gatcggccat gtcctcgacg
     3481 caactgaagg ttgcgaccgc gacgatccgg gcgtcacggt ctaaccgggc cagcacgtac
     3541 cgatcggaag cggccagctc ctcggcccaa gcggcctccg accactgctc ggccggttcg
     3601 aaaccggcgc gttccagcgc catgatgtcg ttgaggtcgg tttcgcgggc gtgggtaatg
     3661 atcattgctt ctccatcttc gccagtcgcg gcagcagcaa cgtcgacttg cgtttcgtgg
     3721 ggacctcggc gtccggacgc cgcaggtaga gcggttcgtg gccggcatca ggcagctggt
     3781 tgccgaatgc cgcgagtagt gccgggtcaa gctcggcggg acccgagcac acaccggcca
     3841 gagcggcccc ggcaccggcc agcgggatcg ccggcacctg atcaggggag gtgacgaacg
     3901 gtccggcgac gcgggcgccg tcggcgccgt agcgcgccca gtagacctcc ttgcggcggg
     3961 cgtccgccac cacggcgaag tccgccgggg cgccctgccg ggcccagtct tgcgcgaccg
     4021 gatccaggct gcacactccg cgcacttcga cgccgagcgc ttccccgagc gtaagcgcgg
     4081 ccaccacgcc gacccgcagg ccggtgaacg ggccgggccc gacacccacc acgatttcgg
     4141 tcaggtcgga cggggtcgcg tccgcttcgg ccaacaactg ctgcaccaga gggagcaact
     4201 gctcggcatg cgcgcgccga tcggcgacca ctgcgcggct cagcgggact ccgtcgcgag
     4261 cgaggcccgc gcggacgtcc gcagccgtgt cgatggccag ggtgagggtc atagcttctc
     4321 cagttctggg cgggcagcgc tccagcggtc gccgaccggg gtgaagaaca cccaccgggt
     4381 ctcgtcggcg tccaggctgc gccggatatc gacctccagg tagccgtcgg ctagcccctc
     4441 cgcgaccccg gcgccccact cgacgagggt caccgagtcg tccagggaca cgtccaggtc
     4501 gaggtcttcg agctcggccg cggagccgag gcggtaggcg tccacgtgca ccagcccggg
     4561 cccgccgacc agggaagggt ggacgcggga cagcacgaac gtgggcgaga tgatggcgcc
     4621 ggccacaccc aggccggcgc cgaggccctg ggtgaaggtg gtcttgccgg aacccaggtc
     4681 gccggtggcg atcagcacat cgccggcctt gacgatcgcg gcgagccggc ggccaagttc
     4741 ctgggtgtcc tcggcggtcg tcgccgtccg gcagaccggg agctggcggg acagggtgag
     4801 gctggtgccc ttgcgtccga tctcgcggaa gccgtgccgc tgccaccagg cgaccacctg
     4861 cgggaattcc tcgcgagcga ccagctcgac gctcctggtg cccagcagcg cgagttcctc
     4921 gagcgcaact tccaccattg cggacgcgat tcccaggcgc tggaagcggg gcgacaccga
     4981 cacccggtgg atgccggccg catcggcgtc cgtgctcacg atgaccacgc cggcgggctc
     5041 ttcgtcgacc aacgcctgta ccccgaagcc ggacgcgagc gcggccgcga ccctttctgg
     5101 agtttcggat agcgccggtg gcggcgggct caccaccgga cgattgccga aggcatcgac
     5161 gatcaaaccg gcgatggcca gggcgtctgc tggagtcggc cgcaccaggc ggagcgtgcg
     5221 accgtccgcg agcacggtct cggccaattc cattcgagat cctccgtagg gccggtcagc
     5281 aaacccgagc gatcttacca gcggacggtc tacaggccct cgtcgactgg ggcgaagata
     5341 gccatcacct cagccaccga ggtggctgcg gtcagggtcg ccaccgatgc agagtcgagg
     5401 aagatgccgg cgatgccgct gagaatgcgg acgtgctggt cgtcggcgcc ggcgatgccc
     5461 accacgaatt tggccgagtt gccgttccag tcgatcccgt ccgggtagcg caccaccgcg
     5521 atcgccgaac gtcggatcag gttctgggca gcgttggtgc catgcgggat ggccagcagg
     5581 ttgcccatgt acgtcgagac ggaccgctcg cgtgccagca tcgagtcgac gtagccgggc
     5641 tcgaccgcgc cgaccttgac caggagctcg ccaacctcgg cgatcgctgc ttcctttgtg
     5701 gtcgcagtgc cggacagccg gaccgcgtcc gggctcagga caccgatggg ggtctcttcc
     5761 gcgaccggct cagccaccgc gtcggggtca ggcgcgggct caggctcaac caccaccggt
     5821 tcaggctcgg gctcgggcac agctgccgga gccggcgtcg gctctactgc tcggcgggcc
     5881 ggctcgttgg ccgagcggcg caccaagagg taccagacgc cagcgatgac gagtgctgcg
     5941 accactaccg acaagatgat tagttcgacc acgtttccgc ccttctatcc aggcgggagt
     6001 ttaccgaaca ccgtccgcga gcccgcgtcc agaccccgcg cgccgagccg tcgcttacga
     6061 agtcggcaca accaagcggg cggccaggtc ctggggcggc aacggtcccg ggtacgcgga
     6121 cgcggtcagc gcctgcacgc ttgccgctgc cagcgcagca tcgaccgggg ccaacccagc
     6181 ggcgagcaaa gtgccgcaga tcccggccag cacatccccc gaacctgcct gcgcggtcca
     6241 ggccacgccg ggcaaagcca cccagatccg atcatcgtcc ggggtggcca cgtactgcgt
     6301 cgcacccttc agcagcactg tgcagttggt acgccccacc gcggccagaa ccgcagccac
     6361 cggatcggcg gcaacctgtt cccgccgcca accgagcagt ccggccagct caccagcgtg
     6421 gggtgtgagc acaacctcgg gccggccacg accgctgagc tggcggatcg cgtccgcgtc
     6481 cagcaccacc ggaacctcac cggcaaccgc gcgcgccacc tcgcccagat cagcacgatc
     6541 gcaccagcca gatcccagca ccagcgcctg cacccggccg tcgccgacca caacgttggg
     6601 aaagcggtcg agcacacgag cggccgcctc cggcgaaccc aggtagcgaa ccatgcccgc
     6661 gccggcgccc accgcgccgg ccaccccgag caccgccgcc ccggggtagc tgggcgagcc
     6721 ggtgtcgaag ccgaccacgc cgcgcgagta cttgtccgag cttgcgtcgg gcaccggcca
     6781 ggccgcggcg agatcggaag cctcccagcg cacgaacgca ggttcgggaa gctcaagacc
     6841 gatatccacc aattcgactt cgccgcaggc cgactgcgcc ggctccatca ggtggcacgc
     6901 tttcagtccg ccaaaggtca cggtgagtgc ggcttcgaag tggggctcgg cagcgaacgg
     6961 cggttccgga gccaacccgg acggcaggtc gaccgccacc accggcacgt tgctgcgggc
     7021 aaccgcacca gcgaactccg ccaccgcccc gtccagtccc ggacgcgaac cgatgcccgc
     7081 gaccccgtca accaccaacg cgtagccacc caaaccgtcg agcgcggcgg cggagtgcac
     7141 ctcacggcca ccagcggcga ggtagtcggc ccaggccgcg gcgtgcacag ccgaaccggt
     7201 gcgccacgcc tcgacccgga cgccgcggcg cagtagccgt acgcccgcgt aaagggcgtc
     7261 gccgccgttg ttgccggatc cgaccaccag cagcacccga cgcccgtagg cgcggatcgg
     7321 ccgggtcggt ttggggatcc cggccttcgc cgggatgacg gtcgggccag agccggtcgg
     7381 gatgacggtg ggaagcgcag gaatgacgga agcgcgcaat cggcgcagga tggctgtggc
     7441 caaccccgcg gcagcacgct gcatcaactc gcccggagcc accacctcga acgctcgcgc
     7501 ttcggcttcg cggatctgcg cgaccgtgta gccgcgttcc atcaggactc gcagaccacc
     7561 atcgcggccg ccaccccggc gtcgtgggtg atcgagaggt gaacgttggc gatgccgagg
     7621 gcagccaccc gctcagccac cgtgccgtag aacacgaagt agggcttgcc caggtcgtcg
     7681 cgcagcacct cgacgtcggt ccacaccagg ccgtggggcg ctcccagcga cttggctagg
     7741 gcttccttgg ccgcgaaccg gccggccagc cgctcggtgg gcgcatcctc gcgctcggcg
     7801 gtcgtgaaca accgctccaa caccccgggc cgctccaccg cccgggagaa cctgaagacc
     7861 tcggtgaggt cgagcccgat gccgacgatc attcgactgt cacgctcttg gccagattgc
     7921 gcggctggtc gacgtcgtag cccttcagcg tcgccagctc gcaggcgaac atctgcagcg
     7981 gggccatcgc caccagcggc tgcaacaacg tggacacctt cggcaaccgg atcagcacgt
     8041 ccgcgtacgg ctcgacctcg tggtccccgt cctcggcgag cacgatcgtc agggcgccgc
     8101 gcgcacgcac ttcctggatg ttggaaacca ccttgtcgtg cagctggtcg cgaccctgcg
     8161 gcggcaccac gatgaagatc gggatgccgt cctcgaccag ggcgatcggg ccgtgcttga
     8221 gctcgccggc cgggaagccc tcggcgtgca ggtaggcgat ctccttcagc ttcaacgcac
     8281 cctcaagcgc caccgggtag ccgacgtgac gaccgaggaa cagcaccgac ttctggtcga
     8341 cgaagcgggc ggccaactcc ttgaccgaat ccaggccgtc cagcacctgc tggatgagct
     8401 tcggcgtgcc ggcgagctcg gacatcacca cacggatctc gtcgtcgaac atcgtgccgc
     8461 gcacctgcgc caggtacagg ccgagcaggt agcaggccgc cacctgggtg aggaagccct
     8521 tcgtggacgc gaccccgatc tccggcccgg cgtgggtgta gatgacggcg tccgattcgc
     8581 gcgggatggt ggcgccgttg gtgttgcaga tcgcgatcac cttggcccgc tgcgcacgcg
     8641 cgtgccggat ggccatcagg gtgtccgcgg tttcgcccga ctggctgatc gtcaccacga
     8701 gcgtggagcc ggtgatgatc gggtcgcggt aacggaactc gctggcgatc tcgacctcgc
     8761 aggccacccg ggtccaatgc tcgatcgcgt acttggccac caggcctgcg tagtaggcgc
     8821 tgccacaagc aacgatgatg atcttgtcga ccagctgcag ttcggcttcg ggaatgtgca
     8881 gttcgtccag cttcagcgct ccgtctgggg tgaaccggcc cagcagcgtg tcggcgaccg
     8941 cctgcggctg ctcgtagatc tccttgcgca tgaaccagtc gtagccggcc ttctccgcgg
     9001 ccgagagatc ccaggtcacc tcgaacgggt ggctctccgc cggggcgccg ttgaagtcgg
     9061 tcacctcgat cgagtcggcg gtgacgtcga ccacctggtc ctggccgagc tcgatcgccg
     9121 agcgggtgaa ctcgatgaac gccgagacat cggacgcaac gaagttctcg ccctccccaa
     9181 cgcccaccat caacggcgag ttgcggcgcg cggccaccag gcgtccgggc tggtcgcggt
     9241 cgatcgcaac cagggtgaac gctccttgca gccgtcggca gaccgcgcgc atcgcgtccg
     9301 ccaggtcggc gccgccggcc acctccagac ccagcagctg ggccgcggtc tcggtgtcgg
     9361 tctgggactt gaactcgacc cccgttgcct ccagctcggc tcgcagcgcg gcgaagttct
     9421 cgatgatgcc gttgtggatc agcgccaccc ggccgttcgc cgacacgtgc gggtgcgcgt
     9481 tcgcgtccgt cggcccgccg tgggtggccc agcggctgtg cccgatgccc gtcccactgt
     9541 ctggcagggg gcgctgcgcg agttcggcat ccagattgga gatcttgccc gccttcttgc
     9601 ggatctcgat gccgtcgtcg cccagcacgg ccacgccagc cgagtcgtag ccgcggtact
     9661 ccatgcgccg caggccggag accaccacct cgcgcgcgac ccgagggccc gtgtatccga
     9721 tgattccgca catggtggtc actgtactgg tgcgtttgcc agactagagc gcgttgccca
     9781 accgggtacc cgaccagcag tgaggtgcga aaatgtccca acccgcagtg gcggcccagc
     9841 ccgccgcccc ggaggaccat ccctacgggc cgtacctcac cctcgaccgg cagcattggg
     9901 cggcgctggc cgcggctcgc ccggacaacc tggacgcggt gacgctacgc cggcttcgcg
     9961 gcatcggcga cccgaccgac gcggccgagg tgcgcgaggt ctacctgccg ctgaccgacc
    10021 tgctcagcct ctatacagag cggacgggcg ccctgttcgc cagcagccac gacttcctcg
    10081 gcctgaccgg ccagcggacg ccgttcgtga tcggcgtggc cgggtcggtt gcggtcggca
    10141 agtccaccac ttcccggctg ctgaaggagc tgttgtcccg gctgccgtcg aagccgcggg
    10201 tcgacctggt gaccaccgac ggcttcctgt tccccaacgc ccagctggcc gaactcggcc
    10261 tgctggagcg aaagggctac ccggagtcgt acgaccggca ccggttgctg cgcttcgtca
    10321 tggacgtgaa gtcgggcgcg ccgaccgtga ccgcaccggt gtactcgcac ctgatctacg
    10381 acatcgtgcc cgaccaggtg gtcacggtga gccagccgga catcctgatc gttgagggcc
    10441 tgaacgtgct gcagcccgcg cgccgccggg ccaacggcag caccgggctc gcggtcagcg
    10501 acttcttcga cttctcggtg tacgtggacg cggccgacga cgacatccgg tcctggtaca
    10561 tcgcccggtt cctgcaactg tgggagaccg cattccgcga tccgttgtcg tacttcgtcc
    10621 gcttctcgga gctgagccgc gaggaggcga ccgcacaggc cggctcgatc tgggacgcga
    10681 tcaacggccc caacctcgac ctgaacatcc ggcccaccag gggacgggcg accgcgatcc
    10741 tgcgcaaggg taccgaccac caggtcagct gggtgcgaat tcggaaggtc tgacgtggcg
    10801 gtcctgccgc tggtggccgc ggacgcggcg gacgcggttg cgctctggca cgacgccggc
    10861 ctcacccgtc cctggaacga cgcgaccgcg gacttcctgc gggcggtgcg cgggccgtcc
    10921 tcggccgtgc tgggcgtccg cgagcaggaa gccctgatcg gcgcggcaat ggtcggccac
    10981 gacggccacc ggggctgggt ctactacctt gcggtggcgg acgcggcgcg cgggcgcggc
    11041 ctaggccgcg agttgatgct ggccgccgag gactggctgg tttccaggga catccccaag
    11101 ctgcagttca tggtgcgcac cgacaacgcc gctgtgctgg acttctacgc gcacctgggc
    11161 tacgcccagc aggaggtcct cgtgctcggc cgccgcctgg atacggcgta cgcttgagga
    11221 attgggcctc gggcttgacc taagccaagt tgcgagttcc agcagcgggc atcgcccggc
    11281 atagttgttc gctcgatcac ccactcgcct gattggatct cgatgcagat caaggaagtc
    11341 ccagccgcct ccctccggca cccccggatg gcgatcgaga cgatcgccgg cgtggagctt
    11401 cccgagtggg accttgtggc cgagcagatg gaccgggtgg aggtggccgc gggtgaacgg
    11461 ctggtgaccc cgggcaagtc tgacccctac gtctatttcg tcaccgccgg cctggtcaag
    11521 gttcagctca acccatcgcc gagcgatccg gcgcagggcg taaccctttt cccgatggag
    11581 gagggcgacc tcttcgccgc accgactgtg ctgcaactac ctgggataca gcgggccgtg
    11641 cagcgcgggt tgcacccgag atcgagctac ctggccgagg tcgatcgcga tatcgcgatg
    11701 cacaccctga tcgcgctgga gccgtcagtg gtgctgcgct gccccggctc cgtgatcgcc
    11761 gaactcgccg agcgacatca gccctgggcg caactgctgg cgaccatcgc gctgctgaac
    11821 aacgccgcgc tcgctcagga cctggcttca cagaagttcc cggccgagca gcgctaccgc
    11881 acgctggcca ccaaccgtcc cgatttggtg cgccgcctgc cgcagcgcga gatcgcctcg
    11941 ctgctgaaca tcaccgattc ggcgctcagc cggatcgcga agcgcgtccg tagcgccgcc
    12001 gagtagggct acgccggctc gagcgagagc cgctccttga ccactccggc cagtcgccgg
    12061 gcgatctcgt ccgcctgggt ttcggtgccg gcttcgacca tcacccgaac cagcggttcg
    12121 gtgccggacg gacgcagcag cacccgtccc gcccggccga gttcgcgggc ggcggccgaa
    12181 acggcggcgt tcaccaccgg atcgatgccg gcgcgcacct tgttgacgtt cgggacgttg
    12241 atcatcacct gcggcagccg gaccatcacc gcggcgagtt cacgcagcgg cttgccggtg
    12301 cggaccatgc gcgcggccag gtgcagcgcc gtcagcacgc cgtcgccggt cgtggcgaac
    12361 tcgctcatga tcacgtgccc ggactgctcg ccacccagcg tgaagccgtt ggcgttcatc
    12421 gcctcaagca cgtagcggtc gcccaccttg gtctggtcga cggcgatgcc ctcggcccgc
    12481 atggcgttga tgaagccgag gttgctcatc accgtggcca ccacggtgtc gctgtgcagg
    12541 gtctggttct cgcgcatcgc cagggcaaga accgccagga tctggtcacc gtccacgatc
    12601 tcgccggtgg cgtccacggc caggcagcgg tccgcgtccc cgtccagcgc gatgccgaga
    12661 tcggcaccgt gctcgaccac ggcggcctgc agcgcaccca tgtgggtgga cccgcaggcg
    12721 tcgttgatgt tcaggccgtc cggggaagcg tgcagcgcca ccacttccgc gccttgggcc
    12781 tcgaatgcgg ccaggccggt gacgtgggcg gctccgtggg cacagtcgac cacgaccttc
    12841 agcccttgca ggctggcgtc cggaccgagg ctggcgacca ggtgcgcgat gtaggactcg
    12901 atcgcgcccg gatcgtccgc gacccggccg acgccggctc cggtgggccg cagccacacc
    12961 tcggccatgc gctcctcgat ggcatcctcg atgtcgtcgt cgagcttcac gcctccgcgg
    13021 gcgaagaatt tgatgccatt gtcgggcatc ggattgtggc tggccgagag catcacgccc
    13081 agatcggcgt ccagttgacc caccagatgc gcgacgcccg gggtcgggat cacgccgagg
    13141 cgaaccacgt ccacgcccgc ggacgcgagc ccggctacca ccgcggcctc aaggaactcg
    13201 ccgcttgccc tggtgtcccg gcccaccagg gcaacgggac gccgggagcg ctggaacgcg
    13261 cccgcctcgc ccagcacgtg ggcagcagcg accgacaatt ccagcgctat ctccgcggtc
    13321 agttcgaggt tcgcggtgcc gcgcacccca tcggtgccga acagtcgagc catcgcccga
    13381 ccgaaatcag cgcttgctgt actgcggcgc cttgcgggcc ttcttcaggc cagccttctt
    13441 gcgttccttg acgcgggcgt cgcgggtcag caggccggcc ttcttcagcg ccggacggct
    13501 ggcttcgacg tccactgcgt tcagcgcacg ggcaatgccc agacgcagcg caccggcctg
    13561 gccggtcaca ccgccgccga caatcgtggc gatcacgtcg taggaaccca tcaccgctgc
    13621 ggtcacgaag ggctcgttga ccgtctgctg gtgcagcttg ttcgggaagt agtcctcaag
    13681 ggtgcgaccg ttgatcgaga actggccggt gccggggagg atccgcaccc gggcgatcgc
    13741 ctccttgcgg cgaccggtgg cagcacccgg ggcgacgacc gccggacggc cgcccggcag
    13801 cggagcgctg gccacggcct cggcgcggta ggcgatctca cgatcgtcct caacgaaggg
    13861 gacgacctcg tcgacggtgg tctcttccac ggtttcggtc acgttgattc cttgactcac
    13921 tgagcgatct gggtgatctg gtacggctgc ggcttctgcg cgctgtgggg atgctcaggg
    13981 cccgagtaga ccttgagctt cttgatcagc tgacggctca gcttgttctt gggcagcatg
    14041 ccccagaccg cacgctcgac cgccttgcgc gggtcggacg cgagcagctc gccgtagggc
    14101 accgccttca ggccgcccgg acgacccgag tggcggtagg ccatcttgtc ggtggccttg
    14161 ttgccggtga gggcgatctt ggacgcgttc accacgatga cgaagtcgcc ggtgtccacg
    14221 tgcggagcga actgcggctt gtgcttgccg cgcagcaggg ttgccgcggt gacggccagc
    14281 cggccgagga cgatgtcctc agcatcaatg acgtgccagt ccctggtgat ctcaccaggc
    14341 ttggggctgt acgtagacac gggtcggttc cctgcttcca taagttgtct caaacggcta
    14401 gtgcgggatc caccaccggc ggggcagacc acagcgcaac agcggtttag attaccctgc
    14461 ccctgctggg gggtcaaaac gcgggttcgc gcgagtcgtg gacggcgaga cgggccaacc
    14521 gacttggtga gttgtaccta gggtttgacc atggcagaca tcctccagat ccgacacgcc
    14581 gccagtggta ccgaggcgga gttggccatc accgatgcca ccatccgggc gccggaactg
    14641 cgccagttca acatcggcgg cgagccgctg gccacctacg acccggggtt catgaacacc
    14701 gcgtcctgcc gctcggcgat cacctacatc gatggcgaag ccggcatcct ggagtaccgc
    14761 ggctacccga tcgcggagct ggccgagaag tccacctacc tggaggtggc ctggctgctg
    14821 ctgaacggcg agctgcccac cgcgtcccag ctttcgggct gggagcaact gatcaccgag
    14881 caccggatgc tgcccgacaa gctgaagacc gtgctggccg gcttcccgta cgacgcgcat
    14941 ccgatgacga tcctgatggc gtcaactgcc gcgttctact ccttctaccc ggacgcgcag
    15001 aacttcaccg atacggaggc gcgggccctg aacatcgccc ggctgatcgg caagatgccc
    15061 accctggccg ccttcgccta tcgcaagggc aaggccaagc cgtacatcaa cccggacgac
    15121 tcctacggct actgccacaa cttcctgcag atgctgttca agcaggccga gcgtcgctac
    15181 caggccgacg agcggctgat ccgggcggtc gaggtgctgt tcatcctgca cgccgaccac
    15241 gaacagaact gctccactaa cgccgtccgt tcggtggcct cgtccggcaa cgacctctac
    15301 acctgctgct cggccggcat cggcgcgctg cacgggccgt tgcacggcgg cgcgaacgag
    15361 gccgtgctga agatgctgcg cgagatcggg tcggtggacc gcgtccctga gttcatcgag
    15421 ggcgtcaagg ccggcaaggt gcggatgatg ggtttcggcc accgcgtcta caagaactac
    15481 gacccgcggg cgaagatcat caagaagtcg gtcgaggacg tgttcgaggt gaccggcgtg
    15541 aacccgctgc tggagatcgc gctcgaactg gagaagtacg gcctcaccga cgagtacttc
    15601 gtgaagcgcc ggctctaccc gaacgtggac ttctactccg ggctcatcta cgaggccctc
    15661 ggcttcccgc cggagatgtt caccgtgctc ttcgcgattc cgcggacgtc gggctgggcc
    15721 gcccagtacc aggagttcat caccgacccg gagaacaaga tcgcccgtcc caagcagatc
    15781 tacaccggtc atcggacgcg cgagtacgtc ccgatggagg ctcgcggctg agacccagcg
    15841 ctcacgacca gcgggcccca cgcccgccgc aggattcccg ggggcaatcc ggtgaagtcg
    15901 gttgcaggcc ctagtcggcc gtagttaggt agtccccatg agttcaaagt tgagcgcagc
    15961 atcggcgcag gtgaccagtc ccgacgacgt ccgcaacgta gttctgatcg gaaacgccgg
    16021 atcgggaaag acgtccttgt tcgagcaact gctcaaggcc cggctcgccg gctaccgcgg
    16081 cgagaaggag gacgccgagc gcgccgccca gctctacctg gcctccatgg tcaccggcga
    16141 cgtgctggtg aacctgctgg acgcccccgg ccaccccgac ttcgtcggtg aactccgcgc
    16201 cgggattcgc gcagcggacg cagccatttt cgtgatctcg gccgcggacg ggatcgatgg
    16261 cgccgcggta accctctggg atgaatgcgc cgcggccagg ctgccgcgca tcatcgcctt
    16321 gaccaagctg gacgcgggcc acgccgactt cgactcctcc gttgccgaag cccaggacaa
    16381 gttcggcgag ggcgtcctgc ccacccacgt gcccacgctg agcgcgccgg gcgtggtcgc
    16441 cggcaacatc ggcctgctca gcctcgagga gcatgattac tccggcggcg aggtggtcaa
    16501 ccgcaagctc accgccgacg actcgcacgt cgagacctac cgcgggccgc tgatcgaggc
    16561 gatcatccag gaggccgagg acgacaccct gatggaccgc tacctcagcg gcgaggagct
    16621 cgacaccgac tacctgctgg acgacctgcg caaggccgtc gccaccgcca acctgtaccc
    16681 ggtggtgccg gtggcgtcca acaccggcgt cggtgtggag gagctgctgc ggctgatcga
    16741 gcacggcttc ccgaccccgt ccctgcaccc gaacccggac gcgaccaccc cgtccggcga
    16801 cccgctgccg aacccggcca cggatccgtc cgcgccgctg gtggcgcagg tgatccgcac
    16861 cacgaccgac cccttcgccg gccggctttc gatggtgcgc atcttctccg gcacgctcac
    16921 caccgaaagc gtcgtgcacg tctccggtca ccggccgaag ccagacaacg cccatcccga
    16981 ccacgacaac gacgagcgcg tgggcatgat ccaggccgcg gtgggcaccg agttgaagcc
    17041 ccgtccgtcc gcgatggccg gcgagatcgt gatggtgccc aagctgacca ccgcggagac
    17101 cggcgacacc ctttcggcga aggaccggcc ggctttggtc gtgccgtgga acctgcccga
    17161 gccgctgctg ccgctggcga ttcgcgcggc cacccgcaat gacgaggaca agctcgccgg
    17221 cgcgctgcag cggctggcgg tcgaggacac caccgtccgg ctggatcgcg gcggcagcga
    17281 ccagttggtg ctgtggacca ccggtcaggc gcacgccgac ctgctgaccg cccggctggt
    17341 cgaccgctac ggcgtgaagg tcgagcagga ggagctgaag atcccgctgc gcgagacctt
    17401 cgtggcgaag gccaccgcgc tcggacgcca cgtcaagcag tccggcggcc acggccagta
    17461 cgcggtttgc aacatcgagg tggagccgct gccccgcggc gccggcttcg agttcgtgga
    17521 caaggtcgtc ggcggtgcgg tgccccggca gttcatccca tccgtggaga agggcattcg
    17581 caaccagctc gacaagggtg tcttcagcgg ctacccgatg gtcgacgttc gggtgaccct
    17641 gttcgatggc aaggcgcact cggtggactc ctccgacatg gccttccaga tggccgggtc
    17701 actggccatc aaggaggccg cgaccccgaa gtccgtggcg ctgctggagc cgctcgacct
    17761 ggtcacggtg accgtcggcg aggagtacct cggcgccgcg atgaccgacc tgaccggacg
    17821 ccgcgggcaa ctgctcggct cggactcgga gaaccacaag gcgatcatcc gcgcgctggt
    17881 gccccagacc gagctggccc gctacgccat cgacctgcgc ggtatcgccc atggctccgg
    17941 cacgttcacc cgcgagttcc atggctacga actgctgccg gccaacctca tggagaagta
    18001 caccaaggcg aactgaccga agacccgttg cgggtcagtg cacgtcgatg aagccgaaga
    18061 cgccgtccgc gaccatctgg acggcaattg cgctcagcag caaaccggcg atccgggacg
    18121 cgagcacggt gcccgactcc cgcagcactg agcggatcag tccggcgtag cgcaggaaca
    18181 cccagaccag cgccatcacc acgaccaggg cggctgccac agtcaggtag ccgcccaggt
    18241 agggcgcgcg ctgcaccgcc agcatcgtgg cgacgatcgc gcccgggccg gccatcaacg
    18301 gcgtccccag gggcacgatc gccacgttca ccccggcgtg gctgaccggg tcctcggtgc
    18361 gtcccatcag caggtcgagg gcgaccagca ggagcagcag cccaccggac acctgcatgg
    18421 ccggcactga gacctgcagg tagtcgagga tctgccggcc gaacagcgcg aagccggcga
    18481 tcacgccgaa cgccaccgcc gaagcccggt tggccgcgcg gcggcgcagc ttctcgtcaa
    18541 gggagttggt gagaccgagg aacaccggca gcagaccggg cggatcgacg atgacgaaga
    18601 gggttaccgc ggtggagatg aagaggttcg gatcgaagaa ctggcccacc ggcccagcct
    18661 atggcctcac aggctgtgca gcggacgggc gcccggcggc atggtgcgag cgtccacgat
    18721 ctccttgccg aacgggaagc aggtcaccgg gatcatcttc aggttggcga tcgccagcgg
    18781 aatgccgacg atcgtcaccg cctgcgccac cgcggtggtc acgtggccga tggccagcca
    18841 gagaccgccg atcaggaacc agatcacatt gccgatgccc gagccgatgc ctgcgctcgg
    18901 cttggcgatc accgcgcgtc cgaacggcca cagcgcgtag ccggccatcc ggaaggacgc
    18961 gatcccgaag gggatggtca cgatcagaat gcaggccagg atgcccgcga agaagtagcc
    19021 gacagccaac cagaagccgc cgaacaagag ccagatgagg ttcagaatcg ttcgcacggt
    19081 tcaatgaaag cgggctcgcg tccatttccg gagggggctg caccctgatc tcacccttaa
    19141 gactgaccgc taaagtgacg cccatgcctg ccgccgaacg gatccaccgc gatgtggtct
    19201 cgtacgtgcg gcgcagcgct cggatgaacg agtcgcagca gcgggcctgg gacgcccacc
    19261 acgacgcctg ggtggtgccg ctatccaccg gcgcccgcag cacttcggtg gcggcggacg
    19321 ccagcgtcga ctgggcgcgg gtgttcggac gcgaggcccg gctctgcgtg gagatcggct
    19381 cgggaaccgg ccacgcgatc accgcgctcg ctgaagcgat gccggacgcg aacctggtgg
    19441 cattcgaggt gttcgcgccc gcggtcgcgt ccacgctggg acggctggct cgccacggtg
    19501 tgaccaatgt ccgcgtggtg ctggccgacg gtgcccaggc tttggccacg gtgttcgcct
    19561 cggcttcgat cagcgaactg tggacgttct tccctgaccc gtggcacaag gcccggcacc
    19621 acaagcgccg gctggtcagt ccggagttcg ccgaggtggt cgcgtcccgg ctgaccgcgg
    19681 acggggcctg gcggctggcc accgactggg ccgactatgc cgaggcaatg cgtgaagtgc
    19741 tggacgccgc ccccgggctg gccaactgct gcgacggctg ggcgccacgc tatgaattac
    19801 gtcctacaac gaagtacgaa cagcgtgggt tggacgccgg ccggcggatc tatgacctcg
    19861 cctaccgtcg ggctggcgta tgacccggta tcggctcgac ctcggctacg acggcgcggc
    19921 cttccacggc tgggccggcc agaccggttt gcgcacggtc cagggcgagc tggagacctg
    19981 gatcggccgg gtgctgcggt taccggctcc cccggtgttg acctgcgccg gacgcaccga
    20041 cgccggcgtg cacgctcgtg gccaggtggc gcacctcgat ctggacctgc ccgaacccga
    20101 actggttgcg gccaccctgg ccagacgatt gcgccgggca ctgcccgagg atgtcgcggt
    20161 ctatcgcgtc cgcctggctc cggaggggtt cgacgcgcgg ttctcggcga tctggcgacg
    20221 gtacgcctat cggttgagtg acattgggcc gctggatccg ctgctgcggg gcaccaccgc
    20281 ggcggtgcgg gcagaactcg acctcacgct gatcaacgcg ggcgcccggc aactgctcgg
    20341 gctgcatgac ttcgcggctt tctgccgggc ccgggagggc gcgacgaccg tccgcgaact
    20401 gcaatggctg gagggcgtgc gcttggcgga cggccggatc gagatcggcg tccgcgcgga
    20461 cgcgttctgc cactcgatgg tgcgctcgct ggttggtgcg ctggtagagg tgggcagcgg
    20521 ccgtcgcgac ctgtcctggc tgagcggcct gctgcacagc ccggaccgag tcggcgacat
    20581 cccggtgatg ccggccgcgg ggctggtgct cgaagaggtc ggctacccgc ccgatgacca
    20641 gctggctgcc cgcgctcagg ccgcgcgcgc cgtccgtacc ttgggagagg aatgcccatg
    20701 agccactact tccacacccc ggacggcccc gcgaacttcc gtgaagtaca actgcgcttc
    20761 tgggacaccg actgggcctt caccaccgcg gccggagtct tctccgcgga cggcctcgac
    20821 ctcggcacgg ccgtgctgct acgcgagtcg accccgccgg cgcatgccag acgactgctc
    20881 gacctcggct gcggctacgg cgtgattgcg gtcgggctcg cgtccgcctg cccggacgcg
    20941 gtggtggacg cggtcgatac caacgagcgc gcgctggaac tgtgcgccac caatgccggc
    21001 cgcgccgggg tggccgaccg ggtgcggccg atgctgcccg aacaggccga tccggacgcg
    21061 cgctacgacg agatctggtc gaatccaccg atccgcatcg gtaaggccgc actgcacgag
    21121 ttgctgctca cctggctgcc ccgattggcg cccggcggcg tcgcccggct cgtggtgggt
    21181 cgcaacctcg gcgccgactc gttgcaggcg tggctgaaca cccagggcta ccgctgcgaa
    21241 cgccgggcca gcgccaaggg attccgggtg ttttcagtgg tcattgaaac tcagtgagac
    21301 tcccgcagac cgggggctgg ggcctaagct gatcgtgatc gttccatcgc acagaatgag
    21361 gatcccgatg accgaagaga acatcacgcc cgagcccgag ggcggcttct tcagcgacct
    21421 gctcgacaag gccaaggact tcggcgagaa ggccaaggac ttcgccgagg acgccttcga
    21481 tgcggccaag gacttcaccg aggacgcggt cgaggcagtg aaggacttcg ccgaagacct
    21541 ccccgagaac gccaagaagc tcgccgacga caccaccgcg gccgtgaagg acttcgccga
    21601 agacctcccc gagaacgcca agaagctcgc cgacgaggcc aagtcagccg tttcacacct
    21661 ggttgacgac aagccggccg atccgcaggc ctgatcttcc aactgcaaac agtgggacgc
    21721 ccacccgggc gtcccacttg tttttttggg tgggcggacg catccggatg ccttaggctg
    21781 atcccacctg aacctttgca ggggaagccg gtgagagtcc ggcactgacc cgcaaccgta
    21841 acggcgagta gatcgcccaa gtcggaatgc ctgcacaagt tctggctcaa cacacccgtc
    21901 gaggcatgcg gggagccgga cccgcgcagt tggcccggat tccagcggcc cgcgcgaaag
    21961 gatcttcatg tcggtcgtcc tgcgacgagc gctggctgtc gggactgcag ccacgctgtc
    22021 actgctcact ctcctacccg ccacccctgc tcaggccgcg ccgcaggctt gcgacggcgt
    22081 ctgggtcgtc gtccagccaa cggacgcgac gagcgccacc atcaagtgcg ccacgaccca
    22141 caccgacgcc gtggtggcca cgcagagcgc gggctacacc gtggcaggct cggacagcgg
    22201 cttcggcttc ttcgtggaac agatcaacgg tgctcccgag cccttcaaca tgaacaccgg
    22261 gccgtggtgg acgctgtgca ccgctgcggt gagcgcggaa ggcgtaatcg gcccgtgggc
    22321 caccagccag accggcgcca ccagcaccgt cccggccaag ggcagcgcga tcggctggta
    22381 cctcccgggc ttctcatgcc cagagctttc gcaggttccg gaggcggaaa gccccagccc
    22441 gacacccacg cccacgccca ccccaactcc gacccccacg cctacggcgc ccgctaccgg
    22501 aaccgcgtcc gtcacctcgg cagccaagtg gctgaacgcc agcgtcccga agaaatggga
    22561 cggggcaggc gctgcgctgg acgtcggcct ggccctggcc acccgggaat gtgacttcac
    22621 caccacgctg ggcgacattc gcgactacct ggtcaagcag gccgatgcct actccaagac
    22681 cagcccgtat gcggccggca agctggcaat cttcgcgtcc gcggtcggcg acgatccggc
    22741 tgacttcggt ggcgtgaacc tggtcaaacg ggtgctggac gcggtcgagg cgagcggccg
    22801 aatcggctcg tccgacttcg cgttcggcca cgcgctggcc atgatcggcc tcgaccgcag
    22861 cggcagtgcc ccgaccgaga agatggtcga ctacctgctc agccagcaac tggcgagcgg
    22921 cgcctggggt ttcggcgcca cggctgatcc cgacaccacc gcgctggccc tgatcgcact
    22981 gtccagcgag gtgatcacgc cgaccgatgc caccaaggcg gccgtggcca aggcagtcgc
    23041 ctgggcggcg accagccaga aggccgaagg ctactgggcg aactactcgc cggtggacag
    23101 cacttcactg ctggcatcgg ccttgaaact gcacggcgtc gataccgcca aggccctcgc
    23161 ctggctgggc acccagcagc tgcgcaacgg agccttcgcc aactcgctga acggcaccga
    23221 agctaaccag ctttcgaccg cgaccgctct ctacctgctc agcggcgtga ccttcgctac
    23281 ggtatcggcg cctctggaca tctgcgccga ggacaccggg gaagcgtccg agtcggcgtc
    23341 ggcggctgcg aagtccgagc agttggccaa caccggcgcg cggggtaacg ccctaccgct
    23401 cgggctggcc ggcctcgggg cggtggcgct cggcggcgtc ctgctgatcg tgcgccgcga
    23461 acggtacgcc gcccggcggg cacagcgatg aggatccgag cctggctggc cggtgccctg
    23521 gccacagcga ttgctgtggt ctcggcaccg ggcgtcccgg cactggccgc gccaaaggcg
    23581 ctgccgaaat cgaccaccag ctgtgccggg gtctgggtga tcgtcgaccg ggggaacggg
    23641 caggtcgcca cccgctgtgc caccaagtac cgcaccggcg tacaggcgct gaccagcgct
    23701 ggattcaccg tcaagcagga cgacggcttt gtgctgcaga tccacggttt cccggccaaa
    23761 ccggacgcca agtcgttcac caagtactgg tcgtactggc acgtcacccg caacgcggac
    23821 ggcacttggg gcgagtggaa gtactccgac aagggcgcga ccacctcgag gccggccaag
    23881 gacgtggccg agggctggcg attcggcggc tcgggaaaga ccgcgccggc gaaggagccg
    23941 ccgcgggcct acctctcgtc ccccactccc acggtcgtag gcacggcgaa agtcgggaac
    24001 ctgctgaccg ttgacaccgg cagatggacg ccgactccgg atcggatcac ctaccgctgg
    24061 taccgctcgg gcaaggcgat caagggcgca accaaggcga cgtacaagat cgccaaggcc
    24121 gaccgcggca agcggctgaa ggtcgtcgtg acggccaggg gcagcggcct gcagaccgtg
    24181 acgaagagct cagcgcagac cgcgaaggtg aagaattgaa gcgttcgctg ctggcgtcga
    24241 tgttcatcgc cctgttggcc ccgctggcga tggttggggc cacgccggca gtggccgagt
    24301 cggtgaaggt ctgttccgga gtctgggtga tcgtcgactt cggcgaactc ggcggcatca
    24361 gcacccagtg cgcgaccaag ttcgacaccg ggaccgccgc actgaagagc gccggcttca
    24421 cgcccaccct cgagaacagc atgctcgtga agctggacgg gaagcccacc aagcccgaca
    24481 tctacaaggc gtattggtcg tactggcagg ccacccgcgc cgacgacggt agctactcgg
    24541 agtggaagta ctccgatctg ggcgcgaaca gctatcaccc caccaagggg aacgccgagg
    24601 gttggcgcta ccaggcgttg gcggacggaa aggtacatcc tgacgcgacc cccccgtccg
    24661 cgccggtggc gacccctgag ccgaccagca agccgtccga gaccgccaag cccagtccca
    24721 agccgacggc caccaagacc gtgaccaagg cgcccacgtc gaccagttcg gcgaccccga
    24781 gcgccaccga ctcccccagc gcgagtgctg cgccgacgac cccgcccgcc acgcccagtg
    24841 agccgaccgg gtggccgtcc gcggctagcc cggcgccgga gggcacggtc aactccccga
    24901 tcgaaccagc acccagcggc accccagtcg gtgcactggt cgccggtggc ctggtgttgg
    24961 ccggcggtgc cggcgtcggc gcctggtggt ggctgaaggg acggcaccgc tgacctcact
    25021 gcacccttgg gcatggtggt gctgggcgat cggaattggg ctcgccgtca gtggcaccac
    25081 caaccccttg ctgctcgcac tcatcgccac cgccatggtc gtggtggtga tgttgcggcg
    25141 cagttcggcc ccgtgggcgc gctcggtgcg ggcctatctg ctgctgagct tgttcgtcat
    25201 cggcatgcgg gtgctgttcc aggtgctgat cggcggcggc accggcgaga ctgtgctgtt
    25261 caccctgccg gagatcccat tgccggaatg ggccgccggc attcggctcg gcggtgcggt
    25321 gacgctcgaa ggactcattt tcacgctgta cgacgcgctg cgcctggcgg tgatgctgct
    25381 ctgcatcggc gcggcgaatg ccctcgccaa cccacggcag gcgctgcgtt cggttcccgc
    25441 agcgctctac gaggcgtctg tggcagtggt gatcgcgctc agcgtggcac cccagttgat
    25501 cgagagcgcc cagcgcgtcc ggaaggctcg gtggcttcgc ggcgacgccg gcaccgggct
    25561 gcgcgcgctc ggggcgatcg cccttcccgt attggcggac gcgatcgagc gctcgatgtc
    25621 gctggccgcc ggcatggagt cgcgaggctt cgcccgcacc caggggctcc cggtcaagcg
    25681 cacgctgccg gtgatgctgc tctcggtgat ggtcgcgacc ctgggtgcgt tcctgctact
    25741 cagcaccact ttgtgggagc tggcggtgac cctgctcatc gtcggcctgg tgggcaccgg
    25801 gtttgggttg cgcgcagccg gcacccggtt gcaggtgacg cgctaccgtc cgcatccctg
    25861 gggttggcga gccaccggcg tggccgcgtc cggcctgctg gccgcggccg tggtcatggg
    25921 gacgggctgg ctcgatccga ccggaggggg cctctggccg tccaccgatc cgctggagtg
    25981 gccagagttg accgcgccga tgctgctggc gatcgctttc gtgctggcgc cgctaccgct
    26041 gacgatcccg cgccggttcg cagcagtgag cagcgtgcgc accgatgtcc gggtgcgtgg
    26101 cttgcgggca gtgagccgat cgtgatcacc ttcgagaacg tcagcgtcac ctacgctggg
    26161 gcggctcggc cgaccctgcg tgatgtgagc ttcacgctgc ccgagggtga tctttgcctg
    26221 gtcgtcggca ccaccgggag cggaaaatcc actctgctag gggcgatgaa cgggttggtg
    26281 ccgcacttca ccgggggcac cctgcagggg cgggtggtgg ttggcggtcg ggacaccgcg
    26341 cgctaccggc cgcgcgacct ggcggacgtg gtgggctacg tcggtcagga tccgctgcgc
    26401 ggcttcgtca ccgacaccgt cgaggacgag atcgcctacg gcatggagca actcggcgtc
    26461 gacccggtgg ccatgcgcaa gcgcgtcgag gagaccctcg acgtcatggg catcgccgac
    26521 ctgcgccggc gtccgctgat cgagctttcc ggaggtcagc agcagcgggt cgcgatcgcg
    26581 tccgtactgg cggcgcatcc gaaggtgctg gtgctggacg agcccacctc cgcgctcgac
    26641 cccaccgcag cccaggacgt gctctcggcc atctcaactc tggtgcacga agtcgggctg
    26701 accgtggtcc tcgccgagca ccggctggag cgggtgatgc atgctgcgga ctcgatgatc
    26761 tggctgcccg gggacggtac cgctcaactc ggcagcccgg ccgctgtgct cagcagcgcc
    26821 gacgtcactc ccccgctggc cgcactggcc cggaccctga gctgggccga ggtgccgttg
    26881 agcgtccgcg aggctaggcg aaaggtgcag gcgcttggac tgtcgctggc cgcacccgca
    26941 actgagccgg ctgctgccag cgacgaccag ccggcgttga ccgcacgcga cgtggaggtt
    27001 cgctacggcg agatcagcgc cgtccgcggg gtcgacctcg acctgcatgg cggcgacgtg
    27061 accgcgctgc tcggccgcaa tggttccggc aagtcgtcgc tgctctgggc gcttcagggg
    27121 gccctgagct gcgccggcga gatccgggtg ggtgcggccg acccgaggaa gctgtccgcg
    27181 gcggacgcgc gacgcgtggt caccctggtg ccgcagaccg cagccgatct gctctacctg
    27241 ccgagcgtgg ccgccgaatg cgagcaggcc gaccgggaga gctcggcgga ggcaggcacg
    27301 gcgcgagcga tcctgcggcg gctcggtgcg gacgtggcgg acgaccacga cccgcgcgac
    27361 ctgtccgaag gccaacggtt ggccctggtc ctggcgattc agctcaccgc ccgaccgcag
    27421 gtgctgctgc tggacgagcc cacccgcggg ctcgactacc agtcgaaggc gcaactccac
    27481 ggcatcatcg atgcgctggc cgcgtccggg gtggcggtgc tgatcagcac tcacgacgtg
    27541 gagttcgccg ccctggccag ccgcaagctg ctgctgatgg ccgacggtga ggtgatcgcg
    27601 tccggctcga ccagcgacct gctcacttcg tcaccggcct atgcgccgca gatggccaag
    27661 gtgttcgcgc ccgtcccggt gctcaccccc caagacgtag ccaaggggct ggcatgacca
    27721 acgtccgctc cctgaacgca cccaccgacg actttgctcc cgaaagggac ccgcgctccc
    27781 gaattatcgg gagcgggagt cccattcggg agcaaagttc gagcagggcg agcacgggac
    27841 gggtcagcgc ggccttgatt gtggcgatgg tggtgggcgc ggtggcgttc agctggccgc
    27901 tgttcctcag ccacacctcg gcgatgtcgg cggcccaggc accgatcgtg ttcgccgtgg
    27961 tgctgccgct ggtgttggcg gttgtgctca ccgagctcac cggcggacgc atggacgcca
    28021 aggcgctggc catgctcggc gtcctggccg cggtcgggac gatcctgcgt ccgctgagcg
    28081 cagggacggc cggcttcgag ctgatcttct tcctaatgat ccttgccgga cgcgtgttcg
    28141 ggcccggctt cggcttcgcg ctcggctcga tcacgctgtt cacctcggcg ctgatcacct
    28201 ccggcgtggg gccgtggctg ccgttccaga tgctcagcgc cggcttcgtc ggcttgttcg
    28261 ccggactgct gccgaaggcg tccgggagga tcgagatcgg gctgctggcc gcgtacggca
    28321 tggtctcggc tttcgcctac ggctggttga tggacttctc gttctggccg ttcgcgatcg
    28381 gcgacggcac ccagctctct ttcaacccgg acgcgtcccc gctggaaaac ctgcaccgtt
    28441 tcgtcctgtt caacctggcg acgtcgatgg gctggaacct gggccgggcg ctgactaacg
    28501 cggtgctgat caccctgctc ggcgtcccgc tgctgcggct gctgcgccga gccgcaagac
    28561 aggcaaactt cgtaccggtg agtttgggtt cgacactggc ggaatagggc aagttaaggg
    28621 caggttggac ctgacaagca aagtaccgac gaagggaaat cccatggcct acacgctgcc
    28681 cgatctcgac tacgactacg gcgccctcgc accgcacatc tcggccgaca tcatggagtt
    28741 gcaccacagc aagcatcacg ccgcctacgt ggccggcgcg aacacggcgc tggagaagct
    28801 ggcggaggcc cgcgagaaga gcgacttcgc gagcgtgccg aagctggaga aggatctcgc
    28861 cttccacctc ggtgggcaca tcaaccactc ggtcttctgg aagaacatgt cccccgacgg
    28921 cggcggcgag ccgaccggcg aggtcgcgtc cgcgatcgac gagttcttcg gtggcttcga
    28981 agggttcaag ggccagttcg aggccgcggc aaactcgctg caggggtcgg gctgggcgat
    29041 gctggtctgg gacaccctcg gccagcggct gaacatcaac cagctctacg accagcagaa
    29101 caacctgccg gtcggccagc tgccggtgct gcagctcgac atgtgggagc acgccttcta
    29161 cctgcagtac aagaacgtga aggccgacta cgtgaaggca tggtggaacg tcgtcaactg
    29221 gaccgacgtc gccgaccgcc tggcaaagag ccgcgccgtc gtcatcttct gacctgaatc
    29281 aacggccccc cgagggcaaa tcctgagaga acttgaccca tctctcaggt tgaggcagac
    29341 ttttctcagg aaactgctcg tcaaccctcg ggggacttcg ttttgaacaa ggcaatcacc
    29401 gcagcggcgc tatccgcgac gctcctgatt ggtggcgcca ccatcgccac cggcctgcac
    29461 aaggacgtcc gcgtgagcgt cgatggcgag tcgctgcccg gcggcgcgtt cgccctcact
    29521 gtgggcgacc tactggccgc gaagggcatc accctgaccg caggcgacga ggtcagccca
    29581 tccgtcgaca cggcaatcag cgatggcgac gcggtcgacg tccgtttcaa caagccggtc
    29641 accctcacgg tggacggcac cgagcgtcgg ttcttcacca ccgcaaccac cctggacgcc
    29701 gcactcacca gcgccgcact gcagggtgag ctcaccgggt cggagatgtc ggcactgccc
    29761 accgccccgg tgccgcgcga cggtctcgag gtcaccgtga gtacgcccaa gcaggtcacc
    29821 ctcgaggtgc gcggcaagga gactgagctc accaccaccg cagcctcggt ggccgaactt
    29881 ctcgccgagc gcaatctgat ggcggacgcg gacgacaagc tctcccccgc cggcgacgct
    29941 gcgatcaccg acggccaaaa gatcaccctc gaccgggtcg aggtatccac gaaggtcaag
    30001 acggagccag tcaagttcga caccgtcgag aagaaggatt ccagcctctg gaagggtgag
    30061 tcccgcatcg tcaccgccgg caaggtcggt ttggcgaagc gcacgtacgc catcaccgag
    30121 gtgaacggcg aagtgaccga gaaaaaggtc gtcaacgaag atgtgctgac caagccggtc
    30181 gaccaggtgc ggaaggtcgg caccaaggtg tccgccaacg gtgtaggcat caaccttgcc
    30241 cgcgccgcga tgtgggatcg catcgccagg tgcgaatccg gcaacaactg gagcatcaac
    30301 accggcaacg gctactacgg cggcctccag ttcaatcgcg cgtcctggaa cgccaacggc
    30361 ggacgcgact tcgcggccct gccacaccag gccagtcgcg ccgaacagat caccgtagcc
    30421 aaccgctact acgcgaaggc cggtacccgg ccctggagct gtgcctgaac cagcctgagt
    30481 aggcctgccc gcccccatga tccgcggccc tgcaaccttc ccctcccccg gcccctggtt
    30541 gcagggccgt gacaacttca tggccacaca aacctgagaa accattgact atcttctcag
    30601 cgtcagccaa gctttttctc agtaacccga cccgccggga cgcccggcct ggaggaactc
    30661 cgatgaagat catcgccacc gcactcttga ccctggcatt gctagccggc ggcgcgccga
    30721 ccgtcgaggc cgtcgcggcg cccgctgaac cagcggcaag cgcgtcccag gcgcccgcgg
    30781 tgtccgtccc ggcgaagccg aagaaggtca cgatcaagga cggcgccgag aagaagcgca
    30841 aggtggagac caccacagcc acagtcggcg atctgctcac cgagctgggc atcacccttg
    30901 gtgagcatga tttggtcaag cccgccgtga cgaagaagct caagcgccac accaaggtac
    30961 ggatctaccg catcacggtg tccaccagcg tcgtgaccga agccgtgccg ttcaaggtcg
    31021 agaaggtcaa gaacccggaa ctggcacgcg gcgtgaagaa cgtcctggtc gccggcgtcc
    31081 ccggttcggc cgagcgcacc tatcaggtca ccacggtgaa cggggaggtg ctgaagaaga
    31141 agcaactgct ggccgagacc gtcctcctgg cgccggtgac ccaggtcgtc gaggtcggca
    31201 ccaagggcaa ggcgctcaat ctggcccgac tgaagctgtg gaacaagatc gccaagtgcg
    31261 agtccggcgg ccgctggaac atcaaaaccg gcaacggcta ctacggcgga ctgcagttca
    31321 atctggccac ctggcgcagc gtcggcggac gcgatttcgc gtcccaccca cacaaggcca
    31381 gcaaggccga gcagatcacc gtggccaacc ggctgtacgc aaagcgtggt agccgccctt
    31441 ggggtggctg cgcatagact tgaaccaccc gacgacggcc ctgcgactcc cctcccctca
    31501 cttcagccgc agggccgttc ttcgtccccg gacgcactgc tagcctcgtc cgcatgcttc
    31561 tgtccgaccg tgacattcgt gcccagatcg atgccggacg ggtcgtcctt gatccctggg
    31621 acgctgcgat ggtgcagccg tccagtgtgg acgtccgcct ggaccggaac tttggcatct
    31681 tcgagaacca ccgctacctg ttcatcgacc cggccgcgga ccagcccgac ctgacccgcg
    31741 aggtggccac gtccggcgac gagccgttcg tgctgcatcc cggcgagttc gtgctcgggg
    31801 ccacctacga gcacatcacc ttgccggacg acgtcgccgc ccgtctggag ggcaagtcca
    31861 gcctcggccg gctcggccta ctcacccact ccacggcggg cttcatcgac cccggcttca
    31921 gcggccacgt gaccctggag ctctcgaacg tggccaccct gccgatcaag ctctacccgg
    31981 gcatgaagat cggtcagctg tgcttcttcc aactcagctc gccggccgag cacccctacg
    32041 gcagcgccgc cgccggctcg cactaccagg gccagcgcgg accgacgccc agccgctcgc
    32101 acctcggctt cagccggacg cagctgccgt cctgatcctt cgctgccgtc ctgagcccga
    32161 gatcaaggca ccctggtcgc cgagaaccag ccaccggtcc cgagaaccag ccacaggtgc
    32221 tggcgttgaa caacgcaggc tggcgttcgc tggtgaaccc tgcccgccgc ccaccgcttg
    32281 atccgtcgca aacgcaagca ccgcccgccc ccagagctgg ggacgggcgg tgcttgcgtt
    32341 ctgcggctca ggccttttca gccttaccgg ccagcacgtt gacgaagccg gcggcttcag
    32401 cggcctcgac cgagttgaac cagacctcgg cttcggtctg gccgtaccac tgcgactcgg
    32461 gggtgtggaa cttcatcgag tcccggttgc ccttgatgtc gtagccggcc ggcggctcgg
    32521 tgcccacgta ggagccctcg ccgaactcgg cggcgtccgc agcctcggcg tccaccgcac
    32581 cagcggcggt cacgtcgacc tcgccgatcg cgtccgcacc atcggcggcc tcggcctcgg
    32641 tgggcacttc ctcgacctcg gcagcagcgg ccttcttcgc cggggccttc ttggtggcag
    32701 ccttcggctc ggccttggcg ggctcggcct tcttggcggc gcccttggcc ttggccttgc
    32761 gggactcctc gacggtctcg gtgacgagct cgatcacggc catcggcgcg ttgtcaccct
    32821 tgcgggggcc gatcttcgtg atccgggtgt agccaccctc gcgctcggcg accttcgggg
    32881 cgatctcggt gaacagcacg tgcaccacac ccttgtcggt gatggtctgc agcacgatcc
    32941 ggcggttgtg caggtcgccc cgcttcgcct tgctgatcag ccgctcggcc agcggctgga
    33001 cccgcttggc cttggcctcg gtcgtcgtga tcttgccatg ctggaacaac tgcgtggcca
    33061 ggttggccag gatgatccgc tggtgtgccg ggctaccgcc gaagcggggg cccttagtcg
    33121 gcttgggcat ggttcgttgt ctcctgtatg gctatggatc agtacgagtc gtcgtagtcg
    33181 acgtcggtgt cgtagttgga cagtgcgctc agcgggtcga agcccgggga gctgtccttg
    33241 agcgagaggc ccatctcgtg caggcgcagc ttgacctctt cgatcgactt cgagccgaag
    33301 ttgcggatgt cgagcaggtc ctgctccgag cgcgagacca gctcgctcac ggtgtggatc
    33361 ccctcgcgct tgaggcagtt gtacgagcgc atcgtgagct tcagctcctc gaccggcagg
    33421 gccaggtcgg cagccagctg ctcgtccacc ggggacggac cgatctcgat gccctcggcg
    33481 tccacgttca gctcgcgggc caggccgaaa agttcgacca gggtcttgcc ggcggacgcc
    33541 atcgcgtccc gcggcttcat cgaggccttg gtctcgacgt ccacgatcag cttgtcgaag
    33601 tccgtccgct gctcgacgcg ggtcgcctcg acccggtagg tgaccttcag caccggggaa
    33661 tagaccgagt caaccgggat gcggccgatc tcggcgtccg ggttcttgtt ctggacggcg
    33721 gacacgtagc cacggccacg ctcgacgacc agttccatct cgatcttgcc ggagtcgttc
    33781 agggtcgcga tgtgcagctc ggggttgtgc acctcgacgc ccgccggcgg ggcgatgtcg
    33841 gcagcggtga ccgcgccggc gccagccttg cgcaggtaca tcaccaccgg ctcgtcctcc
    33901 tcggaggaga gcaccagcga cttcaggttc aggatgatct cggtgacgtc ctcgaccacc
    33961 ccggggatgg tggagaactc gtgctggttg ccctcgatct tgatgctggt cacggccgcg
    34021 cccggaatgg acgacagcag cgtgcgccgc atcgagttgc caagggtgta gccgaagccg
    34081 ggctccagcg gttcgatgac gaaccgggag cggaactcgt cgacgacctc ttcggtaagg
    34141 atcggacgct gtgcgatgag cattcttggt ttcttcctct ccaccgcccg ctatttgacg
    34201 gtgactgatt ctccgggaac cgcccggccg gttgttgaga ccgagcctgg tgaagggctt
    34261 cttgcccctc accaggcccg gttcagctca cttcgagtag agctcgacga tcagctgctc
    34321 gctgacgtcg atgacgatct gctcgcggac gggcagctgg tgcaccagga tccggccctt
    34381 gttgggacgg acggtcagcc agcccggcac caccttgtcg ttgtgggtct cgcgagccac
    34441 gacgagcggg tacaggttca gcgacttttc cttgacgtcg atgacgtcgt aggcgctcac
    34501 ccggtagctc ggcacgttca ccttcttgcc gttgaccgtg aagtggccgt ggacgaccag
    34561 ctggcgggcc tggcgccggg tggtcgcgag accggcccgg tagatgacgt tgtcgagacg
    34621 ggactcgagg atgatcagca ggttgtcacc ggtcttgccg gagaggcgct gggcctcctc
    34681 gtagtagcga cggaactgct tctcgagcac gccataggcg aaacgggcct tctgcttctc
    34741 gcggagctgc agcaggtact cgctctcctt ggtgcggcct cggccgtgca tgcccggggg
    34801 gtacggacgg tgctcgaagg ccttgtcgtt gcccaccagg tcggtgccga gccggcggga
    34861 cttcttcgtg atcggtccgg tgtaacgggc catgttctat ctcctcttcg gctctgggct
    34921 cagacgcggc ggcgcttggg cggacggcaa ccgttgtgcg gcaccggggt cacatcggag
    34981 atcggaccga tctccagacc gacggcaccg agcgaacgga tcgcggtctc gcggccggag
    35041 ccgggaccct tgacgaagac gtccacgcgc ttcatgccgt gctccatggc gcggcgaccc
    35101 gcggcctcgg cggccatctg ggcggcgaac ggggtggact tacgcgagcc cttgaagccg
    35161 acggtgccgg cagaggccca tgagatcacc gcaccggtgg gatcggtgat cgagatgatc
    35221 gtgttgttga acgtgctctt gatgtgcgcc tgaccggcga ccacgttctt cttttcctta
    35281 cgacgcagct tgctcttcgc ggccttctgg ctcgcggtac ggcctgcagt agccaattca
    35341 gttactcctg gatccttgtc ctaggccgcg cgtcagcgcg ccttcttctt tccggcgaca
    35401 gccttcttct tgcccttgcg ggtgcgggcg ttcgtccggg tgcgctgacc gcggacgggg
    35461 aggcccatgc gatgacgacg gccctggtag gagccgatct cgatcttgcg acggatgtcg
    35521 gcggcaacgc tgcggcgcag gtcaccctcg gtctcgtagt tcgcttcgag atggtcgcgc
    35581 agggcaacca gctgatcgtc ggtgagttgg tggacacgga tgtcgccact gatgccagtg
    35641 gcggccaagg tctccttggc gcgggtacgg ccgatgccgt agatgtaggt gagggcgatc
    35701 tcgaggcgct tctcgcgcgg caggtcgacc ccgataaggc gtgccatcag gcggttacct
    35761 ttcaataatc cgagccggtt ccccgactcg cgaaggtgtc tggcgtgatg cgtccccttg
    35821 ccgcctggca tgggccccgg ccttcgtccg ggggtggcat cgacgagctc gtcggcgatg
    35881 gtgcgatcac gtttgcggag tattcagttg tgggtgttcc gcgtcccgcg ctggacgggc
    35941 cgagtagtac tcagccctgg cgctgcttgt ggcgcgggtt ttcgcagatc accatcacgc
    36001 gaccgtgccg gcggatcacc ttgcacttgt cacagatctt cttgacgctc ggctgaacct
    36061 tcatcgagca actttctgac tcatcgccgg acgcaggggt gcccctcgct tggactcggg
    36121 gaccactgga tcgactggtg gtggattctt gcggacttgc ctaccggtgc cggaacacga
    36181 tgcgaccacg gctcaggtca tagggagaga gctcgacgac cactcggtcg gccgggatga
    36241 tgcggatgta gtgctgccgc atcttgccgc tgatggtcgc cagaacccgg tgtccgttgg
    36301 ccagttccac gcggaacatg gcattcggca gggcttcgac gacggttccc tccagttcga
    36361 gggcgccttc tttcttcgcc attactccca atcgttgctg tgtgtgggcg cacgcgcgtg
    36421 caagcacagg tcgtgcaagg gtcaatcgta cggcaggagc gctagtcgcc ccaaatcgcg
    36481 gcgacagctc ggctgcgacg cagaacgcgt ccgattgtcg ccgacacgcg ggtcgaaccg
    36541 aaaactgcac ccagcagacg cgattcgcgc cgttgcgacg ccttctttat acctagtgac
    36601 acgatgcctt ggattacgat accttggtat tcgaggcaaa ggagtcgcgg tggctatcga
    36661 caagcagcta ttgaagggga ccatccccct gctggtgctg cacctgatca gccagggcga
    36721 gatgtacggc taccagctga tccgctccct caacgacgcg tccgcgggcg agttcaagtt
    36781 ctccgagggg accctgtatc cagtgctgca cgccctcgag cgggacggcc aactgcgcgc
    36841 ctactggcgg gtcgccgaca ccggacgcaa acgcaagtac tacgccatca ccgctgccgg
    36901 cgtccgctca ctctcccggc ggaccgccga atggcggacg ttcgccggcg cgatcgaggc
    36961 ggtcctcagc gaggagatcc cccatgacgc aaccaatcgg aggaactgaa catgaaccgc
    37021 gtgctatcca gctggccgta cgtcctgttc gtcgcgttgt gctggctgag ctgggaactg
    37081 atcggggtgc tcgagttccc gggcatggac tccgcgcagc agaagagtct cttcctcacc
    37141 atttcgctct tcgtctatcc cgtgctgctg ttccttgcgg gcaccgcgct cggctaccgc
    37201 cgcggctatg actgggtcac cgtcctggcc tgcctggtgg tgttcatcgt gtgtcaggtg
    37261 ttcagctcgg cagtgaaggc ctacccgctt tcgatcggcg acctggccac cttcagcgcg
    37321 tactacctga tccccgccca cctcggcatc gccgccgggc tgggcatccg cgccgtccag
    37381 cgacggaatg cgcaacccaa ggagtgacca tgcccgaggt gcccgaacta catgccttcg
    37441 ccagtcgagc cacggcgggc atcgccgccc cggctgagcg ggccggcgcc acgtccgagc
    37501 tgtatgagca tgccctcagc gcctacgagg aacaccgcgc gtccgggttg gcgcacgacg
    37561 atgcggtcca ggccgcgctc acgcaactcg gcgaaccgtc cgagtaccgc aaggacttcg
    37621 cgagagcgca tcgggcgcga ctcaccccgc gaaccgtgct gctgatcggc ggtgcggcga
    37681 tcgtcttcat gatcctgctc tgggcgttct tcgcgctgat cttcggggtg gcgtcctgag
    37741 ggatgcgcac cccggcaggt gtgcgaacct gttccaactc ccggctgcgg ttgggctggc
    37801 ggcgtagact tgtccgtcgc gcacctaagc cgtgaggaaa ccaagtggat tctgttggga
    37861 caccccccac cactctcgaa agggaactgg cggtcgaaca ggcacacgtc gatctcgtct
    37921 accggcactt ggcgaaggcc gccaagtcgg cccgcaaggt cgcgtcccag ggccgcaaga
    37981 tctatcgctc cgaccgtgag acctgggtgc gggaggagga cggcaccgcg ctcttcgagc
    38041 gggacgcgtt cgccttccag gccgcccgac ggctcgccgt cctggacgcg gagcatgagg
    38101 gtctggtctt cggccggctc gaccacaacg atggcgagat ccgctacatc ggacgcatcg
    38161 gcgtccgcga cgacgagtac gagccgctgg tgatcgactg gcgtgcccgc gccgccgaac
    38221 cgttctaccg cgctactgcg accgacccga tggacgtggt gcgccgacgc gtcctccgtt
    38281 gtcgcaacga catcgtggtg ggcatcgagg acgacctgct ggacagctcc cactcccacg
    38341 acgacctggt gatcgtcggc gagggcgccc tgatggcggc gttgaagcgg gcgcgcggcc
    38401 acaagatgcg cgacatcgtg gccacgatcc aggccgagca ggacgaggcg atccgcgccg
    38461 attacccggg cgtgaccgtg atctccggtg gtccgggcac cggcaagacg gtggtcgcgt
    38521 tgcaccgggc cgcctacctg ctctactcga accggcgccg cttcgaacgc ggcggcatcc
    38581 tggtgatcgg gccgagcagc ctgttcatga actacatcga gcgggtgctg ccgtccctgg
    38641 gcgaagactc ggtgaccctg cggccgatcg gcggcgtcgc ggtggacacc ctcggcttca
    38701 cggccgcccg aatcgactcc gcccccgcgg ccaccgtcaa gggcagcctg cggatggtgt
    38761 cgctgctgaa gcgcctggtg aacctgccgt ggctcgatcc gatgcacgag caggcgccac
    38821 tgcgggtgac cgtcaagggc gaggtgctca gcctgtcgga gacctcgctg tcgcgcatcc
    38881 ggcgggacgt gctcacccga accaaggcga acgccggccg cgcccaggcc gaagccgcag
    38941 tgatggacgc gctgtacgcg cagctgcccg cagaactgga gatggaccag gacacgatga
    39001 ccgaactggt cagcgattcc gccgcctatc ggatgttcct gcaggcgtgg tggccggatc
    39061 tgaccgcgac ggacgtgctg cgccggctcg cgtccgagcg gatcgtcggc cgggtgagcg
    39121 agggcatcct ggacgctgtc gagcaggccg agctggctgc gtccttttcg ttcgaggatt
    39181 ggtcggtcgc cgacgtcgcg ctactggacg aactggtggc gatgctcggc ccggtcgccc
    39241 acgacgagac cgaagagccc ctggtgttcc tgccggacgg caccgagatc aacgaggtgg
    39301 tgaccaccgc cgaccggctg cgcgaccatc gtgaggtcga tccgcacgcc gatccgcacg
    39361 acaccttcgc ccatgtgctg gtggatgagg cccaggacat cagcccgatg caatggcgga
    39421 tgctgcggcg gcgcggttcg caggcgagtt ggacgatcgt cggcgacccc gcgcagagct
    39481 cgtggccgga cttggcggaa tccgatcggg ccctgaccga gttgatcggt tcggcgccgc
    39541 accggaactt ccggctgtcg acgaattacc gcagcccggc cgaggtgttc cgccttgccg
    39601 cggacgtggt gcgccgcgat ttcccgggtg ccgacctgcc ggacgccgtc cgctcgaccg
    39661 gtatcgagcc gctgctgttg gctgccggcc cagatgagct cgcggcgcgc ctggacgctg
    39721 tggtggcgga gctggccggt caggtgcagg gcacgatcgg cgtgatcgcg ccgccgtccc
    39781 ggttggctgg gctggctgca gcggagctgc cgtccgtcgc tgcgcagggc tctcgggtga
    39841 gcttcatcag cccgctgact gcgaagggcc tggagtacga cgcggtcgtg gtggtgggcc
    39901 cggacgagat cgtcgcggag tcccctggcg gcgtccgcgt gctctacgtg tcgctcaccc
    39961 ggcccaccca gcgcctagtg accatcgatg tcggccagtc aggtgcctgg cggcgcgatc
    40021 tcgcgtagcc gggccaggat ctcggcggtg gccgacgacc ggttctgggt gaagaactgc
    40081 agcccgggag cgccgccgga gagcagccgg tcgcatagtt cggtggcgat ctcgaggccg
    40141 accgcccgca ccgcggccgg atcatccgcg accgcgtgca gcctcgccac tagtggttgc
    40201 ggcagatcgg cgcccgacag ggtcgcgaag cgttcgatct ggcggacgtt ggtcacgggc
    40261 atgatcccgg cgatgatcgg cagctggcaa ccgagggcac ggacgcggtc aaccagacgg
    40321 aagtaggcgt cggcgtcgaa gaacagctgg gtgatcgcga actcagcgcc ggcctgctcc
    40381 ttctcggcga gcacccgggc gtcgagttcg aagtcgtggt tctccggatg tgcgtccggg
    40441 aaggcggcga ccccgacaca gaagtcgccg gcggccttga ccagccgaac cagttcggtc
    40501 gcgttgttca gcccatcggg atggcgctcc caagccgcgg tcggaccgcc ggtcgggtca
    40561 ccacgcaccg cgaggatgtg ccgcactccc gcgtccgtgt aggcgtcgat cgctccgtgc
    40621 aactcggcga cgctctggct gacgcaggtg aggtggccca tcgtcagcgc gccggtggtg
    40681 gattggatca ggcgggtggc gtcgatcgtc cgtgcccggg tggagccgct ggcgccatag
    40741 gtgaccgaga caaaatcggg ccggaggggt tcgagcgcgc tgatcgcgtc cagcaattgg
    40801 tcctgaccgg cgtcgtcctt gggcgggaag aactcgaacg acatcgtcgg gtggtcgcca
    40861 gcggccagga tttcggcgat ggactgcggt cgcgacgaag gcatgggggc aagggtaggc
    40921 cagcgtccag tccgcgagcg gtctaggctc agagcgtggt caatctggac gctgcggacc
    40981 cgctctccgc cgaactgagc gccgcgatcg gcgacgagct ggcgggtttc atcgactcgc
    41041 gggcggacgc gttgagcgcg ctccagcctg agatcggcga gctggtcgag ctggcgaggg
    41101 cgttcactac cggaggcaag cggctgcgtc cggcgttctg ctgctggggc tatctggctg
    41161 ccgttggcgg cgataccgtt cctgactacg tgattcgcgc cgcggcgagc ctcgacctgc
    41221 tgcacgtcag cgctctggtg cacgacgatg tgatggacgg ctcggccacc cgccgagggt
    41281 tgccggcggc gcacgttcag ttggccgcac ggcacgctgc gtccgcacgc cggggcgccc
    41341 cggaggactt cggtcgggcc ggcgcgatcc tgctcggtga cctgctgctg atgtggtcgg
    41401 tggagttgtt cacgtcctgc ggcgcaccgc ccgaactgct ggcgtccgcc cagccgctga
    41461 tggctgcagt gcgagccgag gtcaccggcg gccagtacct ggacatctcg gcgcagacgc
    41521 gctcgccgct ggatgcccga cgctcgcccg aagccgtgct tgcagaggtt cgccgggtgg
    41581 tcgagttcaa gaccgcgaag tacaccgtag tacggcccct gcaggtcggt gcggctctcg
    41641 gtggcgggtc ggccgcgctg ctggacgcgc tggcgagcta cgggtcgccg ctggggcggg
    41701 ccttccagta ccgcgacgat gtgctcggcg tgttcggcga cgctgcgctg actggcaagc
    41761 cggccggtga cgacctgcgc gaaggcaagc tgaccgtgct gatcgcgaac gcgatggcgt
    41821 ccgcttcaag cggagcggct gaagagttga gcgggctgct gggacgcgag ttgcctgcgg
    41881 cagaggtgga cgcggctcgc gagctgatca ccgaatcagg agcgctcgcc gcgaccgaac
    41941 gcgagatcgc ggacgcgacc cgcgctgctg tagacgcgct ggcggacggt ccgatcgcag
    42001 ccccggccgc cgaagggttg cgcaagctcg ctgaactggc cacgcagcgg aagtcctagc
    42061 cgtcaggtgg ttttcggtag tcggcgttcc aaaggatctg gctgagtttg tcactgcctt
    42121 gccggaaggt gccgttgcgt ccgacccggt agtggtagcc gccaggtgat cgccagtgga
    42181 acacgccggg gg
//
LOCUS       DAOPRQ010000010        42007 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_704_length_42007_cov_13.234292, whole genome shotgun sequence.
ACCESSION   DAOPRQ010000010 DAOPRQ010000000
VERSION     DAOPRQ010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 42007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 42007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42007
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_704_length_42007_cov_13.234292"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(349..1782)
                     /locus_tag="PKJ61_02185"
     CDS             complement(349..1782)
                     /locus_tag="PKJ61_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HOC12671.1"
                     /translation="MPSRHLSLRNSEPPVLAEGLRRLRASLGVPGGFPADVLAEAEEA
                     ARRPVLPERDCTDIEFVTLDPEESMDLDQAFHLSRRGDGYLLRYAIADVAAFVTAGGR
                     VDEEAHVRGETLYAPTKRTPLHPAALSEGAASLLPGHDAPALIWELALDSSGETVDTK
                     VSRGMVRSRAKLSYTGAQADLDAGRGGEQLELLRTVGQLRMEIEAARGGVSLSVPEQE
                     IVATDWHWTLAFRAALPIEDWNAQLSLATGMAAAALMLEAKVGILRTLPPARDSSLAK
                     LRRTAAALGIKWPVGMAYPEFLRSLDASVPVEAAMLNACTLLFRGAGYQAFNGSVPEL
                     PEHAAIAAPYAHATAPLRRLVDRYTGETCVAICAGRPVPEWTAAALPDLPPLMAESGR
                     RAKKYEHGTVDLVEAMVLAPSLGRTFTGTVLEVDPEDDYGVLQLPDPAVEAKIRGSEL
                     VLGEQISATLVAADLLAGKVEFRVLPT"
     gene            complement(1792..2295)
                     /locus_tag="PKJ61_02190"
     CDS             complement(1792..2295)
                     /locus_tag="PKJ61_02190"
                     /inference="COORDINATES: protein motif:HMM:NF024848.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase-like protein"
                     /protein_id="HOC12672.1"
                     /translation="MARAPIKPRIGPPLFEIADAAASPSAESSGRRLVIEADGGSRGN
                     PGPAAYGALVLDAETGEVLNSEGLGLGRATNNVAEYSGLIAGLEMAHAIDPNAAVDVR
                     MDSKLVIEQMAGNWLVKHPDMVPLVARARQLRPARVTWSWVPREQNKAADALLNAALD
                     GQPRPRR"
     gene            complement(2295..3038)
                     /locus_tag="PKJ61_02195"
     CDS             complement(2295..3038)
                     /locus_tag="PKJ61_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleic acid-binding protein"
                     /protein_id="HOC12673.1"
                     /translation="MKAAATAQLRLLDLQANDTAIAQFEHRRRSLPEHAAIAEARSAR
                     AKLAEALVAARTKVADLQLEQDKAEADLVPVRERRVRDQERVDDGSITDPKQINAMLD
                     EIAHLGRRISDLEDIELEVMEQVEAATAEHDKLAEELSELESSARALIASRDTQVAEL
                     DDQIREHQAIRDGIAAEIPADLLALYDRIRVRAGGTGVAPLKGRRCGGCQIEATPIAL
                     QTYASAAPDDVVRCEECERLLVRTGAVDA"
     gene            3114..4598
                     /locus_tag="PKJ61_02200"
     CDS             3114..4598
                     /locus_tag="PKJ61_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013161474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HOC12674.1"
                     /translation="MTDQPAPEPAALPNRQTPFSEAFRAFIPEGWADNPAEAPKALAT
                     IASAAAHRRALAEAFPGERLVIPAGGLKVRANDTDYRFRPHSAFAWLTGLGADREPDA
                     VLVLEPTDAGHEATLYFKPRAPRTDPEFYADSRYGEMWVGMRESLAEMTALTGLSCAP
                     ISEFDAALAKNFGATSVRLLAEADQAITDRVQSLRIEAGIVAKETDAAFEVALSEARL
                     VKDAFERDQLRQACADTAVGFEAVVADLPEAIRRGRGERWVEGVFGLHARHLGNAVGY
                     DTIAASGDHANTLHWIRNDGEIRPGDLLLLDAGVEVESLYTADITRTLPVSGTFSPTQ
                     RKVYEAVLAAQEAGIAAARPGAKFADIHAAAIAVIAQYLEEWGLLPVSAAESLDPATG
                     GHHRRWMVHGTSHHLGLDVHDCASARQENYRDGELKAGMVVTVEPGLYFKSTDLLVPE
                     EFRGIGVRIEDDIAITADGAEILSNQLPRDPDAVEAWIAGIWAR"
     gene            4878..6194
                     /locus_tag="PKJ61_02205"
     CDS             4878..6194
                     /locus_tag="PKJ61_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012923732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HOC12675.1"
                     /translation="MSDPKQMKVVLDESQMPTHWYNIVADLPTPPPPHLHPGTQQPLV
                     PEDLAALFPMGLIEQEASTERWIEIPQEVHDIYRLWRPSPFYRAHRLERAIGTTARIY
                     YKYEGASPVGSHKTNSAVPQAYFNKLAGRSRLTTETGAGQWGSALAFACQVFGLSCDI
                     WQVRSSYEGKPYRHVLMETFGASVVASPSELTEAGLALREQFPDTTGSLGMAISEAVE
                     AAAKDENASYSLGSVLNHVALHQTVIGQEALLQLKLFGERPADYLFACAGGGSNLAGI
                     SFPFIRENLEGRSETKIVACEPKAAPSLTEGEYKYDFGDTAHLTPLLKMYSLGPDFVP
                     DPVHAGGLRYHGMSPLVSHCYHEGLLDAIAVKQLDAFQAGVLFARAEGIVPASESNHA
                     IAGAIAQARAATEAGESPVIVIGVSGSGQLDLPAYAEFLAGNMADS"
     gene            complement(6601..7098)
                     /locus_tag="PKJ61_02210"
                     /pseudo
     CDS             complement(6601..7098)
                     /locus_tag="PKJ61_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002549326.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7095..8036)
                     /locus_tag="PKJ61_02215"
     CDS             complement(7095..8036)
                     /locus_tag="PKJ61_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012922968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HOC12676.1"
                     /translation="MTINRAAPRRTILAVPGSSDRFIEKARTLAVDALFLDLEDAVAP
                     AVKVESRQRIAAALNSGAGFAAGLVTVRVNGWESPWTYGDLIEVVSSAGARIDALVLP
                     KTQSAAQVQALDLLLTQVEHNAGLPVGRISLEVQIEDAVGLLHVNEIAAASPRLASLV
                     FGPGDFMASLGMGGLNVGAQPDGYPADAFHHVLMSILVAARAHGLQAIDGPFVAIRDV
                     PGFRRSAELSAALGYDGKWVLHPAQVDAGNEIYSPKAADFERAQRISAAYAEATALAG
                     GAVGAIVVDDEMVDEAGVKLAAAILARGRAAGMEESR"
     gene            complement(8091..8537)
                     /locus_tag="PKJ61_02220"
     CDS             complement(8091..8537)
                     /locus_tag="PKJ61_02220"
                     /inference="COORDINATES: protein motif:HMM:NF014026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HOC12677.1"
                     /translation="MGLAADEVGLDYEVVETPDSADVAIVRLPAPFESRSDLFLEAWF
                     HQGSLEFPPGLIVRLARIAAHCPLLVDVTCDRPAVLTPLLDVAAAITVSYGTGGGAYF
                     DAVTGRVPPRGRLPFDLPRSMADVRTHPEDQPGFDDPLFAFGHGLG"
     gene            complement(8540..9940)
                     /locus_tag="PKJ61_02225"
                     /pseudo
     CDS             complement(8540..9940)
                     /locus_tag="PKJ61_02225"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
     gene            complement(10274..10810)
                     /locus_tag="PKJ61_02230"
     CDS             complement(10274..10810)
                     /locus_tag="PKJ61_02230"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOC12678.1"
                     /translation="MGDGAEGEKLPDLLKDFQAANPDVKITVTPIPWDAAHDKFTSAI
                     SAQKTPDVAMIGTTCMGEFAGLDALEPTPASIDKSKFLAGAQATTEVGGTSFGIPWHV
                     ETRLVYYRTDLAEKAGFSTPPTDWDGLEAMAKAIRDKAGAKWGINLQPGGTDSWQTVT
                     GRLARPARDPGEVVPAFH"
     gene            complement(11114..12256)
                     /locus_tag="PKJ61_02235"
     CDS             complement(11114..12256)
                     /locus_tag="PKJ61_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020385688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HOC12679.1"
                     /translation="MSSLVDEVQAALAKVIDPEIRRPITDLGMVQSVDVADDGRVALT
                     VLLTIAGCPLASTIEADVNEAVLSVPGVSAVDLTLGTMSDEQRAELKRTLRGHDDREI
                     PFSKPDSLTQVILVASGKGGVGKSSVTVNLAMALAAKGKSVGVLDADIYGHSVPQMLG
                     LMDASPTSVDGMIMPVPSMGLRVISIGMLKSSRDQVIAWRGPILDRALQQFLADVYWG
                     DLDFLVVDLPPGTGDVALSLGQKLPNAEVLVVTTPQQASAEVAERAGTMASMLEQKVI
                     GVVENMAFLDLACPHCGQNHRVDVFGSGGGSEVAGTLSTRLGYEVPLLAEIPIDPQLA
                     AGGDLGVPLVATGPERPAAKALLALADRLAGRKRNLVGFKLGVTPV"
     gene            complement(12452..12946)
                     /locus_tag="PKJ61_02240"
     CDS             complement(12452..12946)
                     /locus_tag="PKJ61_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016456506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HOC12680.1"
                     /translation="MAKQDRLDTPGRRSGWIPRMRLEDDAFGSFAESAARYMGTGQFI
                     LWMSVFIIAWLTWNLVVPDAPDRWPFQFLTLALSLQASYAAPLILLAQNRQEARDRVS
                     AEHDREVAAQSRADMDFLAREIASLRMRMNDVATRDFIRSELRDLMVELNEREEAEAK
                     SKPS"
     gene            complement(12939..14198)
                     /locus_tag="PKJ61_02245"
     CDS             complement(12939..14198)
                     /locus_tag="PKJ61_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002515179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOC12681.1"
                     /translation="MTPASSIFVSRIKGLPMLDPNGEQVGKVRDVVIQNRSGKRPPRV
                     KGLVVELFALRRIFFPMERVKSIDANQVIISGLVNTVRFQVREREVLVVDALFDRTVT
                     RAGASQPETIFDVAIKQNRHHDWVLAEVALRESSRRPFQRGHVTIVDWSEVPDFIEKP
                     APRDAEQQVMRLSELKPADVARELHDMDPLLRAAVVQAMDDDRLAEALEELPEDEQVE
                     VIASLDTERAADVLEEMDPDDAADLIAELDSELAETILEQMEPEDAKDVRTLLTYDAA
                     TAGGLMNPEPVILNADATVADALAAVRRQEITPAMAALAFICRSPLDTPTGRYLGAVH
                     IQRLLREPPSVLAAGLIDGDITPLTPQSNIAQVSRYFATYDLVCAPVVDTERRLLGAV
                     TVDDVLDHILPSDWRGVQLDRLEVSDG"
     gene            complement(14245..14709)
                     /locus_tag="PKJ61_02250"
     CDS             complement(14245..14709)
                     /locus_tag="PKJ61_02250"
                     /inference="COORDINATES: protein motif:HMM:NF014470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HOC12682.1"
                     /translation="MPFNFDMSEILVLVVFAVILFGPEKLPELARKTGRVLNYVRNIA
                     NDAKGQIGKELGPEFENLSLADLSPKALVNKHILNPVQAELDPVKAELGPIRSTLTGA
                     AQSPATLAATTTAGSASGREPIATVAPAPEVVATSAVPARLALAVAFDTEAT"
     gene            complement(14748..15629)
                     /gene="dapA"
                     /locus_tag="PKJ61_02255"
     CDS             complement(14748..15629)
                     /gene="dapA"
                     /locus_tag="PKJ61_02255"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HOC12683.1"
                     /translation="MTALPFGRLQTAMVTPFNSDGSLNLSEAKRLAAHLVDVQGNDAL
                     VINGTTGESPTTTDAEKLAVLEAVLEEVGDRARILAGVGTFDTHHTLELARQAERAGA
                     HGLLVVTPYYSRPPQDALADHFLTVADATTLPIMIYDIPHRAGVPVETATMIQIAAHP
                     NIVAVKDAKGQPVASAQVIAATDLAYYSGDDAMTLPLLAVGGVGLVGTSTHFTGQASK
                     AMIGAYAAGDVAGAVARYQALLPVYLGVFATQGCMLVKAGLAARGFAPGPVRKPLLDA
                     SAEQTAAFVAILDAAGL"
     gene            complement(15640..15861)
                     /locus_tag="PKJ61_02260"
     CDS             complement(15640..15861)
                     /locus_tag="PKJ61_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020385673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding carrier protein"
                     /protein_id="HOC12684.1"
                     /translation="MSHVVSAELVANVLSVEVVAGQQVQATDPVVVLESMKMEIPVLA
                     DVAGTVSEIVVRVGDVVRDGDPMVVITTL"
     gene            15944..16603
                     /locus_tag="PKJ61_02265"
     CDS             15944..16603
                     /locus_tag="PKJ61_02265"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HOC12685.1"
                     /translation="MNPSPKTPGRASTLPATIAFAEAFAPESDAAESARERAKQLGLE
                     CISSGAATTLTFLATAVGAKTVVEIGTGTGVSALALLAGMAADGVLTSIDGEAEHQLA
                     AREVLAAGGVATRRVRLIAGQPLNVLPKLTDGAYDLVLIDGDPLEYVEYVAQAEKLLR
                     SGGLLALHHALWHGLVADERNDDDEPLIIREALAAVSTNEAFTPALLPIGDGLLVAVK
                     R"
     gene            complement(16813..18039)
                     /gene="glgC"
                     /locus_tag="PKJ61_02270"
     CDS             complement(16813..18039)
                     /gene="glgC"
                     /locus_tag="PKJ61_02270"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HOC12686.1"
                     /translation="MTSGPNVLSIVLAGGEGKRLMPLTADRAKPAVPFGGTYRLIDFV
                     LSNLVNSDLTKVCVLTQYKSHSLDRHISVTWRMSVLLGSYVTSVPAQQRTGKQWYQGS
                     ADAIYQSMNLINDEDPDYVVVFGADNIYRMNVEDMVKAHIDSGMDASVAGIRVPRREA
                     SAFGIIDAAPDGKINAFLEKPADPPGLPDSPDESFASMGNYVFTRKAFVEALNDDAAV
                     ADSRHDMGGNIIPAFVSSGRAQVYDFTLNNVPGSTEKDRNYWRDVGTIEAYHAAHMDL
                     VSVEPEFNLYNRKWPIWTSQAQLPGAKFTLRGTAEDSIVGSGCIISGGDVDRSVLSPN
                     VMVDKWAKIEESVIYSGCRIGRNAVIRRAILDKNVIVPDGVEIGVNPDHDRARGFHVE
                     GGITVVAKNMAVPGNW"
     gene            18175..19371
                     /gene="glgA"
                     /locus_tag="PKJ61_02275"
     CDS             18175..19371
                     /gene="glgA"
                     /locus_tag="PKJ61_02275"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HOC12687.1"
                     /translation="MALRVSILTREFPPHIYGGAGVHVGQLVPQLRKFTESVEVQCLG
                     EPREGATAHAEDFPPGANAALRVLGADVSMANAIGDVDVIHSHTWYANFAGLIGSKFR
                     DVPHVVTSHSLEPHRPWKAEQLGGGYRVSSWAEKTAFEDAAAVIAVSAGMRGDVLASY
                     TDLDPDRVHVVKNGIDPDEFKPDQGTDVVDALGVDRDRPLVVFVGRITRQKGLVHLVR
                     AAKAFDPDTQLLLLAGAPDTPEIAAEFEGAFAELSKARSGNVKWVQEMLPRASVRQVL
                     THATVFACPSIYEPLGIVNLEAMACETAVVASAVGGIPEVVVDGKTGTLVAYDPKQAG
                     DAEYVANFEAQFAEQVNRITRDPALARRYGVAGRQRCIDEFSWEKIAQETVAVYERAI
                     AYHQSK"
     gene            complement(19460..19630)
                     /locus_tag="PKJ61_02280"
     CDS             complement(19460..19630)
                     /locus_tag="PKJ61_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012923066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3117 domain-containing protein"
                     /protein_id="HOC12688.1"
                     /translation="MAAMKPRTGDGPIEVAKEGRVIAMRIPAEGGGRLVIEMNATEAA
                     ELKTALEGVVVA"
     gene            complement(19724..20032)
                     /locus_tag="PKJ61_02285"
     CDS             complement(19724..20032)
                     /locus_tag="PKJ61_02285"
                     /inference="COORDINATES: protein motif:HMM:TIGR03544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HOC12689.1"
                     /translation="MELFIWLIAVAILGLAAVAASGRLGELPATVTDTPTPHVPTGDL
                     TAADLRGLRFAVVPRGYSMQQVDEVLERLAGQLERAQAVPVDPPAPPASEAPSNTPSA
                     "
     gene            complement(20108..20866)
                     /locus_tag="PKJ61_02290"
     CDS             complement(20108..20866)
                     /locus_tag="PKJ61_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HOC12690.1"
                     /translation="MSKQIRTQGPVIRRHDQVLPTTTDQRLLTSKGDGAWAHADPWRV
                     MRIQAEFVEGFGTLAEVGPGIGVFGSARTPRRDPMYTAASRIGRGLAEAGYATITGGG
                     PGVMEAANKGALEAGGVSVGLGIELPFEQSLNKYVSLGINFRYFFARKVMFLKYSQGF
                     VVMPGGFGTFDEVFEALTLVQTRKVTSFPIVLFGSSFWTPLLGWLQDQVLESGYIAPT
                     DLQLTQVTDDPEEAVALVTGPGPNARPESSAHMS"
     gene            complement(20871..21749)
                     /locus_tag="PKJ61_02295"
     CDS             complement(20871..21749)
                     /locus_tag="PKJ61_02295"
                     /inference="COORDINATES: protein motif:HMM:NF016370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HOC12691.1"
                     /translation="MIDLAALLADHEGILLASQHRARKSSLSRWCLAGRIVRVLPGVY
                     VHPDAADDLVTRLRALVARVPDAIVVGAAAAKLTAWPNEKVATIEAITATTRVAKHGY
                     RFIRRRVAPQQLRHRGPVTLLCAAQVAVDSAATDLGDRIDLLLRKGYPPAEFEAALAA
                     SPKRAGNRVRRRVVNRSKTRPFSRAERILHDLLDRHHMRGWVANDAVVACGANYEIDV
                     AFRERKLALEVDGYEFHSSRAAFEADRHRNNDLTEADWTVLHITWAMLMADPERVIAR
                     IRAVLARRRKRRSPLD"
     gene            complement(22072..23127)
                     /gene="dapE"
                     /locus_tag="PKJ61_02300"
     CDS             complement(22072..23127)
                     /gene="dapE"
                     /locus_tag="PKJ61_02300"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013161489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HOC12692.1"
                     /translation="MTLDLTTDLVTLLRQLVDVESVSGNEGPLADLVEAALRGQPHLA
                     VVRDGDTVVARTDLGRAERVVIAGHLDTVPVAGNLPSTVVGDRVYGRGTCDMKGGVAV
                     ALACAAALAAPSRDITWIFYDHEEVAAEDNSLTRVAREHPEWLAADFAVLMEPTSARI
                     EGGCQGTLRFVLRTAGTAAHSARAWLGHNAIHELAEPLRRLAGYQPRQVVVDGLTYRE
                     GLNAVAVSGGIAGNVIPDAASVTVNFRFAPDRDEADAKQHCLDLFEGYRLEFIDFALG
                     ARPGLGRPIAQEFLAAVGGEPSAKYGWTDVARFAALGVPAVNFGPADPGKAHADDEFT
                     PVADLYTCRDALLRWLG"
     gene            23270..24196
                     /gene="dapD"
                     /locus_tag="PKJ61_02305"
     CDS             23270..24196
                     /gene="dapD"
                     /locus_tag="PKJ61_02305"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017936017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HOC12693.1"
                     /translation="MTRPAHGWGLASVHASGQVLDTWFPNPTLGASTTNEPPKQLVAA
                     ALVDDEVRGVRLDPVLIEIDLDAPPAGVSDAYLRLHLLSARICAPRTINLTGVFAVLP
                     NVVWTNYGPCAVEGFEDVRLGLLGSRGRVSVFGVDKFPRMTDYVVPSGVRIADADRVR
                     LGAHLASGTTVMHEGFVNFNAGTLGSSMVEGRISAGVVVGDGSDIGGGASIMGTLSGG
                     GQEQITIGERCLLGAESGVGISLGDDCVVEAGLYVTAGTKVTLSSGAVVKAAQLSGQN
                     GLQFWRNSVSGTVEARTRAGTGVELNSVLHTN"
     gene            24204..25064
                     /locus_tag="PKJ61_02310"
     CDS             24204..25064
                     /locus_tag="PKJ61_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC12694.1"
                     /translation="MKGLRSSLFAVLGAVVVLAIIAGGVVLWSTYNKAPLPIDDRCVA
                     IAGGNTATIDPAQAHYASIIAGMTIKRDLAPRAASIALATAYQESGIRNLDYGHADSI
                     GLFQQRPSKGWGTIEEIMDPWYSAREFYRAMERIDDWENKDINDVAQAVQRSAYPDAY
                     RRHETNARTLASSLTGETPASFRCQVAAPNTADPDGMGRLLTKTLGADNVSIKAVGGE
                     LTVTAANATHAWAVANIAVANTAEFGLAAVQLGEYSWTHSSRTLAEWVGTGPKSDTEV
                     KLQFAVPTPR"
     gene            complement(25221..26000)
                     /locus_tag="PKJ61_02315"
     CDS             complement(25221..26000)
                     /locus_tag="PKJ61_02315"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOC12695.1"
                     /translation="MPTIDGSTANIPLISLAIQRLTGVSETDADNAVNTNGTPAAWRN
                     LVAGAVDLLVVYEADEAVKAEVESSGVKLEAHPIGRDALVFFTNSSNPVESLTTKQYR
                     DIYTGKISNWQQVGGANAKIVGYQRAEESGSQALLRKYVVGQAKLTKAPTDRITAEMG
                     EIIDGVASYANTGNALGYSVYYYLANMYAVDGIKMLGVGGVQPTKETIADGSYPYAND
                     FFAVVRADEPAGSPARRVLEWLTSEAGARTVADAGYVPLRA"
     gene            25999..26175
                     /locus_tag="PKJ61_02320"
     CDS             25999..26175
                     /locus_tag="PKJ61_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC12696.1"
                     /translation="MSARVNEKSACALALSVLALGVVDPDGTIGEVTEADGVGPEATH
                     PDRTTANAVAANRK"
     gene            complement(26183..27664)
                     /locus_tag="PKJ61_02325"
     CDS             complement(26183..27664)
                     /locus_tag="PKJ61_02325"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HOC12697.1"
                     /translation="MITLLTLAGCVATGPAITSPPTATSIAPSATGGTSAEPSAPASS
                     TIPGEYRGSSAMPAADGDPMLWPTIELRVVTDYGSQAYERYGFVDVNGQLVVKPRFDN
                     YQYCPDAHGRAAFVIGERGDHRAEVLDLTGKLMLRTPAADAGCAGSDHVVFTDVISGE
                     TGEYNSGVLQLSTGRIVVPLAKDRHIEVVQGEIVNVSDPEGEYFLDLSSGARTPHPGW
                     LWYSALEPGAPGLPAAAKRPDYQSTSTFGYVSLAGQWLVEPRFADANPFVAGRAIIGD
                     GTSETLIDADFNPVSGPWTSITVETDANDALLGYHVFRGEQEGLLGPDANVILPVSPS
                     PIYCNLAGACTTVSADGLGKLVLLPELTRTDLPAGLTKAISRDIVSDSVTDPNTDDDL
                     QRNLTRLHFLADGRSVTSAGWSFCDLIGTAWVMCAPSVVTLPPDVFDTRGNRTTFRSV
                     RALAAASGSVPEYYWAVAGRYQGLVDATGAWRYRESRFTQLED"
     gene            complement(27800..28906)
                     /gene="dapC"
                     /locus_tag="PKJ61_02330"
     CDS             complement(27800..28906)
                     /gene="dapC"
                     /locus_tag="PKJ61_02330"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002514073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HOC12698.1"
                     /translation="MSRLAGRLPDFPWDSLDAARARAAAHPDGIVDLSVGTPVDPTPE
                     IAQRVLADTADAPGYPTTWGTPALRRAIIDYLAGRWSARGLVDDGVLPVVGTKELVAL
                     LPTLLGVGAGRTVVIPTTAYPTYDVGGRIAGAKVVACDDPAEVAALKPSLIWINSPAN
                     PHGQILTPQRLREWVAAARGVGAVLASDECYAEFGWDAEPISVLHPEINGGSLANLIA
                     VHSLSKRSNAAGYRAGFLAGDPILVSELREVRKHAGLMVPTPVQAAMVSLLGDHAHVE
                     EQRARYQRRRARLRPALAAAGYRVEHSEGSLYLWCTRDEDCRASVEHLAGLGILVAPG
                     DFYGDAGREFVRVALTATDERVAAAAKRLLAAAG"
     gene            complement(28903..29223)
                     /locus_tag="PKJ61_02335"
     CDS             complement(28903..29223)
                     /locus_tag="PKJ61_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016456517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HOC12699.1"
                     /translation="MTYVITLPCVDVKDLACVEECPVDCIYEGNRMLYIHPDECVDCG
                     ACEPVCPTEAIFYEDDVPSDQAEYYDINVHFFDEIGSPGGAAKLGPIDADHPTVAALP
                     PQAV"
     gene            29328..29810
                     /locus_tag="PKJ61_02340"
     CDS             29328..29810
                     /locus_tag="PKJ61_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC12700.1"
                     /translation="MSVRRVRPDGYFSDLIGFVVAASAAELRLQDRRGETHQVPWAQV
                     HAWRPVGVARGRDPMRTPLAELDRQARLAGLSGRYYVARLSDLLDSREPMPGSPPSAP
                     PPSPAEHVGEWVVAAPTADLIALAWWAAHRDARSLLLVAGDPADHERLLSLGMVARLD
                     "
     gene            complement(29962..31689)
                     /locus_tag="PKJ61_02345"
     CDS             complement(29962..31689)
                     /locus_tag="PKJ61_02345"
                     /inference="COORDINATES: protein motif:HMM:NF016202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HOC12701.1"
                     /translation="MPPSKGLSTTAKIGIGVGAGLIALLGGGYVAGHFLAGENLPVKS
                     SVNGVQLGGLNSAQAVAKLEKELAAASAAPISVKAGDESMSLKPAEAGLAVDYEASVA
                     QAGGGSSWNPVHLLTVLFGGAEQAAVLKVDQAKLNAAVDALAAKVDVEPVNAGVAYSG
                     LKPVVSDAKAGVAVDRAATRAAIQAAYLKAEPVTAPLATAEPAVTTTEAQDVADGLAK
                     SAIGEAVTVSVADKGKLNLQPAAIAASLKFEPKDGSLVPSFDSKTLASQVAKQLSGLG
                     LKQPKNAGYTISKGKPKVVPSVDGVGVDPEQLGAALVPVLSQASGRSASVEVSAQPAA
                     VTTADVEQLGVKKVIGKFTTYFPGTAYRYNNIGKAAKLINGTFLLPGETFSMNQTLGK
                     RTTAAGWMAGGAIDGGKIVERLGGGISQATTTTFNAIYFAGLKDIYHKPHSLYFNRYP
                     VGREATLDWESVDMKFKNDSPYGVLLQASITGKTGSQGSVTVKVWSTKRYTIKSTTPV
                     RSNYRAPGKTVYDDSDGCIAQSAMSGFDVKFYRLFYEGEKLLKKEPFKWSYNSLTPVV
                     CGKDPAKKD"
     gene            complement(31723..32607)
                     /locus_tag="PKJ61_02350"
     CDS             complement(31723..32607)
                     /locus_tag="PKJ61_02350"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOC12702.1"
                     /translation="MASALWGFAFVAQRVGAAHVGPFSFTAVRFALGALVLLPIIWVR
                     DRVRGVPRAKRTAATRAVVLPGIASGVLMTIAVNLQQVAMSDTPAGNAAFITGLYMVF
                     VPVIAAFRGHRSNLATVLGVVASLVGLYLISVNDELTIGRGEWLLILSTVFWALQILV
                     IEHFAGRLSALRFALVQFLTCAVVSSVVALLAESAPFGGLVEAAIPLLYGGLVSVGIA
                     YTLQVVGQRHALATHASLIMATESVFGALGGALLLSEFMGLRGYAGAALMIIGIVVAQ
                     LGTPSARSADPEPLTSYT"
     gene            complement(32677..34758)
                     /gene="tkt"
                     /locus_tag="PKJ61_02355"
     CDS             complement(32677..34758)
                     /gene="tkt"
                     /locus_tag="PKJ61_02355"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002536317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOC12703.1"
                     /translation="MMTEFTWTELDERAVDTARILAADAVEKVGNGHPGTAISMAPLA
                     YLLYQKVMRHDPADPKWVGRDRFVLSIGHSSLTQYCQLFLAGYGVEVDDLAALRTEGS
                     LTPGHPEFGHTAGVECTTGPLGAGVADAVGFAMAARRERALLDPATPLGESSIFDRTI
                     YCIAGDGCMQEGVSGEASSLAGAQKLGNLILFYDDNRISIEGETQIAFTEDVSARYAA
                     YGWHTQHVDWTGGGTSYTENVQALHDAIEAAKAVTDKPSFIKLTTVIGWPLPTKAGHH
                     SVHGAKLGADEVAALKTQLGFDPAANFAVEAEVLAYTRKQAADRAAAAKAEWTPKFEA
                     WRAAAPAQADLFDRLVVRGLPDVQGALPVFAPGKKSTRAASGEVLTALADVVPELWGG
                     SADLAGSNNTTMKGQLSFLPPEQSSHDFKGDYTGRTLHFGIREHAMGNIVNAITQSGL
                     TRCYGGTFLVFADYMRGAVRLAALQKVPSIFVWSHDSVGVGEDGPTHQPIEHLASLRA
                     IPGLDVIRPADANETAQAWAAALANSGRPSAIILSRQDLLVVDRDQPGVAAASELTKG
                     AYTLVDADGELKVVLIATGSEVEVALAARDLLQAEGVGTRVVSMPCQEWFSAQSAEYR
                     ASVLPVGVAKVSVEAGSPMGWREYVGDAGEIVAIDHFGESAAAGVLFAKYGFTAANVA
                     ERARTALSRLG"
     gene            34954..36468
                     /locus_tag="PKJ61_02360"
     CDS             34954..36468
                     /locus_tag="PKJ61_02360"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HOC12704.1"
                     /translation="MKRLVILGGGTAGTMIANKLRAALGKHELHITVVDRDDAHHYQP
                     GYLFMPFGIYNTSDIVRSRHKFISDGVDLVLTEIDRVDPAYRVVHLADGRTLSYDVLV
                     IATGVTPRPDATPGMQGKLWGEKVHEFYSLPGAQALTDAMRRFRGGRLVVHITEMPIK
                     CPVAPMEFTFLADDWLRKHGIRERTEVVYVTPLDGAFTKPVASRELATALTDRQITLE
                     TDFAVESIDNDAAELVSFDGRRIGFDLLVTVPLNMGADYIARSELGDELNLVPCDQHT
                     MVSLAHPEIYVLGDAGTLQTSKAGSVAHFAVDVFVHNFLAEYRGEPATHGFDGHANCF
                     IESGNRKGMLLDFNYTTEPFTGNFPLPVVGPFKLLKETHLNHLGKLAFRWIYWNMLLP
                     GRPIPMIPTNMSLAGKRVEKEVSAEATGLPSIGAPSSPPPPRPAAPAAKPKLEGRSAP
                     ARPKAAPLAKPKGAATPSQAKPKPAPSGGYVDRPGVDSTADPATVLPNPIIPKF"
     gene            36477..36791
                     /locus_tag="PKJ61_02365"
     CDS             36477..36791
                     /locus_tag="PKJ61_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HOC12705.1"
                     /translation="MPTTLIAGRPIDVNDEGFLVAYDQWDEKLGGELARLIGLELTDA
                     HWAVIRFLRADYSLRSETATLRRASTVGGFPIKELFELFPGKPAKKMSYISGLPKPKG
                     CV"
     gene            36812..37360
                     /locus_tag="PKJ61_02370"
     CDS             36812..37360
                     /locus_tag="PKJ61_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HOC12706.1"
                     /translation="MTDAPIVPDFSGPDCPAPNAAPAQRKLVIICSKGNLDMAYPGLI
                     LGSAALGEGIETHLFFTFWGFDMITKGRMDDLKFTMLGNTAMHLTQAPGMPIPAMLGT
                     LPGMTKMATKMMQKQIADLEIPTVPEMLEQIHEMGGHMWACRMSFDMMRLNEADLYEG
                     VEAVISATDFMELSEGAQIIFI"
     gene            complement(37412..38488)
                     /locus_tag="PKJ61_02375"
     CDS             complement(37412..38488)
                     /locus_tag="PKJ61_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008600068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HOC12707.1"
                     /translation="MRYVVIMAGGSGTRLWPLSRQGTPKQLLPLVQGKSLLRLAFERA
                     LASVPAEQVWVVTGAAYADVVAADLPEVPAGNILGEPIGRDSLNAVAWPAAVLARQDP
                     EAVIAQVTADQVIEPPKVFAECLETAFALAESDASALVTLGVVPTSPHTGYGYLQRGE
                     AIDGFPGAHRVLRFVEKPPIELAETYLASGEYWWNAGMFVWRATTLLDQLRQLLPATH
                     ASVLELAEHPDRLAEIFPGLAKTSIDYGVMEPVSGGKGSAHVVAVALPISWRDVGGYA
                     SLAEILATDASGNAISGLSVALGANDNVIVNADEGSVIGLLGVSGLVVVRTPHATLVA
                     RAEDAERIKELVAAVTEQAGAQFA"
     gene            38658..39356
                     /locus_tag="PKJ61_02380"
     CDS             38658..39356
                     /locus_tag="PKJ61_02380"
                     /inference="COORDINATES: protein motif:HMM:TIGR03089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03089 family protein"
                     /protein_id="HOC12708.1"
                     /translation="MENPIIAALTDRVRRAGSEPLLTWYRPADGQRTEFSVKSFANWV
                     DKTANLLDTLGVEGTVAGSLNTSHPGHWMSLVWPLAAWQHGIAYAVGSSDQAELIVVG
                     PEDPQPSPGALTIACSLHPLGLGLCNLPDGVLDFTTEALAEPDAHWAAPVVETNRAWA
                     DAGLARTHAQLADLPPEPTRVLVRPTTPWATLAAALVSPLLGGGSAVVVDGAASEADL
                     ARIAATERAPRSMA"
     gene            39392..40237
                     /locus_tag="PKJ61_02385"
     CDS             39392..40237
                     /locus_tag="PKJ61_02385"
                     /inference="COORDINATES: protein motif:HMM:NF024748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOC12709.1"
                     /translation="MTSMSRRSLLASAAALAGGLAIAGCAPSIPKPSASPTGPITSQL
                     DEVLKVVSGGSDKFGVFVRDVRSGGTYSFNGDYASQNASMVKVMIALMAQRQARADDS
                     TLSQENTDLVTKMITHSDNDAAEQLWPYAGGADAYQQLADELRMSHTHRDPNRPDWSW
                     TWTTPSDQVQLLDYLVTGGTDAFTAAEGKFVYDLMGQVQDDQTWGVGAPKSGTVQVHL
                     KNGWVQFKSSDGLWAVNSMGTVQGEGRDYRMAVMARVADFDTGRELTSEVGRQVFSIL
                     GSGSL"
     gene            complement(40434..41903)
                     /locus_tag="PKJ61_02390"
     CDS             complement(40434..41903)
                     /locus_tag="PKJ61_02390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HOC12710.1"
                     /translation="MASTAQPAGSVLASPQRRSQGIALRRGLTLLGMSVLVPGSAQLA
                     VGKRNVGRVALRVWLTLLAVLVVALLLLIPFRTQIIGLYANPITLTVLRWLVLALGLG
                     WILLVLDAWLLANPRAMSTAGKLVSGGVALALMATGGAVSWTVSGLFAAQSKLVTNIF
                     TGGGSSKVENGRINILLMGGDAGADRQGLRPDSMTVASIDADTGRTVLFSLPRNLQHA
                     PFPSDSPLHAKYPNGYFCHVEDLADACLLNGVYTLAEQNKKLFPGVKYPGAETTAAVV
                     GETLGLKINYWAMIDLKGFQQLIDAVGGIRLDIGKKVPIGSLHGPKGVYGWIKPGKNV
                     KLDGFHALWFARSREYSTDYERMIRQKCVMNAMLKQLNPTTVLTKFQDIAAAGEQIVA
                     TNLPSGEIGTALDLAMKGKSMPMASVSFTPPLIVPMDPDLAKIRAVVTEKIADSEALD
                     AHPESTASSSASGSASPTPKKKPSSTQTDNLDAVCKVSA"
BASE COUNT         6933 a        14331 c        14016 g         6727 t
ORIGIN      
        1 tcaccccggg tgggcgttac ccaccaccct gctctgtgga gtccggactt tcctctgctc
       61 acgcagcgat cacccaaccg actcgtccgc cgcaaggata ccgccaacgg cctgtctggc
      121 tggcccgcgc cgacttgccc cgcccaggtt tcgggacaaa cctgccgcga gtggcgagat
      181 tctcggactt ggctggaccg aaggcaatcc agtggcagaa atgtcccgaa gtgaacggga
      241 ccactggatc gcctggcagc tagagggcgc tctggcggcg ctccgagggc ccacaccgac
      301 ccgggtgtcg ctgggcgagc ccagatcggc ctgtgcagac gcggacgctc aggtgggcag
      361 cacccggaac tccacctttc ccgccagcag gtcggcggcg accagcgtcg ccgaaatctg
      421 ttcgccgagc accagttccg atccccggat cttcgcctcc accgccgggt cgggcagctg
      481 caggacgccg tagtcgtcct cagggtcgac ctccagaacg gttccggtga aagtgcggcc
      541 caggctgggc gccaacacca tcgcttcgac caggtcgacc gtcccgtgct cgtacttctt
      601 ggcgcgccgt cccgactcag ccatcagcgg cggcaggtcc gggagcgcag ccgcagtcca
      661 ttccggcacc ggccggcccg cgcagatcgc cacgcaggtt tcgccggtgt agcggtcgac
      721 cagccggcgc aacggcgcgg tcgcgtgcgc gtagggcgcg gcgatcgcgg catgctcggg
      781 aagctccggg acgctgccgt tgaacgcctg gtagccggcg ccacggaaga gcagcgtgca
      841 ggcgttgagc atcgccgctt ccacgggcac ggacgcgtcc aggctgcgta ggaactcggg
      901 ataggccatc cccaccggcc acttgatacc gagggcggcc gccgttcgcc gcaacttggc
      961 cagcgaactg tcccgcgccg gcggcaacgt gcgcagaatg ccgaccttgg cttcgagcat
     1021 cagcgccgcc gcagccatgc ccgtggccag cgagagttgc gcgttccagt cctcgatcgg
     1081 cagcgccgcc cgaaaagcca gcgtccagtg ccaatcggtg gcgacgatct cctgctccgg
     1141 caccgagaga ctgaccccgc cgcgagcggc ctcgatctcc atgcgcaact ggcccacggt
     1201 gcgcagcagt tccagctgct cgccgccgcg accggcgtcc agatcggcct gcgcgcccgt
     1261 gtagctgagc ttggcccggc tccgaaccat gccgcgggaa accttcgtgt ccaccgtttc
     1321 gccggagctg tccagggcca actcccagat cagtgctggc gcgtcgtggc cgggcagcag
     1381 gctggccgcg ccctcggaca gcgccgcagg gtgcaggggc gtccgcttgg tcggcgcata
     1441 gagggtctcg ccgcggacgt gcgcctcctc gtccacgcgg ccgccagcgg tgacgaacgc
     1501 agccacgtcg gcgatcgcgt agcgcagcag gtagccatcg ccacgccgcg acaggtggaa
     1561 ggcctggtcg aggtccatcg actcctcggg gtcgagcgtc acgaactcga tgtcggtgca
     1621 gtcgcgctcg gggaggaccg gacgccgggc cgcctcctca gcctcggcca gcacgtccgc
     1681 ggggaagccc cccggaaccc cgagactggc tcgaagccgc cgtaaccctt cggccaggac
     1741 cggcggctcg ctgttacgca gcgacaggtg gcgactcggc atggttcctc ctcagcgacg
     1801 tggacgcggc tgcccatcca acgcggcgtt cagcagagcg tccgcggcct tgttctgctc
     1861 cctcggcacc cagctccagg tgaccctggc cggacgcagc tgccgcgccc tggccaccaa
     1921 cggcaccatg tcgggatgct tgaccaacca gttcccggcc atctgctcga tcaccagctt
     1981 cgaatccatc cggacgtcga ccgccgcgtt cgggtcgatg gcgtgcgcca tctccaggcc
     2041 tgcgatcaat cccgaatact ccgccacgtt gttggtggcc ctgcccaggc cgagtccttc
     2101 ggagttgagc acctccccgg tctcggcgtc cagcaccaac gcgccgtacg ccgccggacc
     2161 cgggttgcct cgcgagccac cgtccgcctc gatcaccagc cggcgtccgc tcgactcggc
     2221 ggacgggctg gccgccgcgt ccgcgatctc gaagagcgga gggccgattc gcggcttgat
     2281 cggggctcgg gccatcaggc gtccacagcg ccggtgcgca ccagcaaccg ctcgcactcc
     2341 tcgcagcgca ccacatcgtc cggtgccgcg gacgcgtacg tctggagcgc gatcggggtg
     2401 gcttcgatct gacaaccacc gcagcgccga cccttcagcg gcgccacgcc ggtgccaccc
     2461 gcgcggacgc ggatgcggtc gtagagcgcg agcaggtcgg ccgggatctc ggcggcgata
     2521 ccgtcccgaa tggcctggtg ctcgcggatc tggtcgtcca actcggcgac ctgggtgtcc
     2581 cgggacgcga tcagcgctcg cgccgaactc tccagctcgc tcagctcctc ggccagcttg
     2641 tcgtgctctg cggtggcggc ctccacctgc tccatgacct caagctcgat gtcctcaagg
     2701 tcggaaatcc ggcggccgag gtgggcgatc tcatcgagca tggcgttgat ctgcttcggg
     2761 tcagtgatcg agccgtcgtc gacacgctcc tggtcacgga cgcgacgctc acgcaccggc
     2821 accagatcag cttcggcctt gtcctgctcc agctgcagat cggcgacctt cgtgcgtgca
     2881 gccaccagcg cctcggccaa cttggcccgg gctgacctgg cctcggcgat cgcggcgtgc
     2941 tctggcagcg acctgcgccg atgctcgaac tgggcgatgg cggtgtcgtt ggcctgcaag
     3001 tcgagcaagc gaagctgggc cgtagcggca gccttcatct gagcctcctg gagttgtgca
     3061 cccgactcta gtgtgccgtt ctgacatcca gcgacgggga tagcattcca gccatgaccg
     3121 accagcctgc acccgaaccc gccgctttgc ccaaccgcca gactcccttc tcggaggctt
     3181 tccgggcgtt catccccgag ggctgggcgg acaacccggc cgaggctccg aaggcgttgg
     3241 ccacgatcgc gtccgcggct gcgcaccggc gagcgttggc cgaggcgttc cccggcgaac
     3301 ggctggtgat cccagccggc ggcctcaagg tgcgggccaa cgacaccgac taccggttcc
     3361 ggccgcattc ggccttcgcg tggctgaccg gactgggcgc cgatcgtgag ccggacgccg
     3421 ttctggtgct tgagccgacc gacgccggcc acgaggccac gctctacttc aagccgcggg
     3481 caccgcgcac tgacccggag ttctacgccg attcccgcta cggcgagatg tgggtgggca
     3541 tgcgcgagtc gctggccgag atgaccgcgc tgaccggctt gtcctgcgca ccgatctcgg
     3601 agtttgacgc agcgctggcc aagaacttcg gcgccaccag cgtccggctg ctggccgagg
     3661 ccgaccaggc gatcaccgat cgcgtccagt cgctgcggat cgaagccggt atcgtcgcga
     3721 aggagaccga cgcggccttc gaggtggcgc tttcggaggc ccggctggtg aaggacgcgt
     3781 tcgaacgcga ccaactgcgt caggcctgcg cggacaccgc ggtcggattc gaggccgtag
     3841 tggccgatct gcccgaggcg atccgtcgcg gccgcggtga gcgctgggtg gagggtgtat
     3901 tcggactgca tgcccgccac ctgggcaacg cggtcggcta cgacacgatc gccgcgtccg
     3961 gtgatcacgc gaacaccctg cactggattc gcaacgacgg cgagatccgt ccgggcgatc
     4021 tgctgctgct cgatgcgggg gtggaggtcg agtcgctcta caccgcggac atcacccgca
     4081 ccctgccggt ctcgggcacc ttcagtccca cccagcgcaa ggtctacgag gcggtcctgg
     4141 ccgcgcagga ggcagggatc gccgccgccc gcccgggcgc gaagttcgct gacattcacg
     4201 cagccgccat cgcggtgatc gcgcaatacc tcgaagagtg gggtctgctg ccggtgagcg
     4261 cggccgaatc cctcgacccg gcgaccggcg gccaccatcg gcgctggatg gtgcacggca
     4321 cgtcccacca cctcgggctc gacgtgcacg actgcgcgag cgcccggcag gagaactacc
     4381 gcgacggtga gctgaaggcg ggcatggtcg tcaccgtcga gccggggctg tacttcaaga
     4441 gcaccgacct gctggtgccc gaggagttcc gtggcatcgg cgtgcggatc gaggacgaca
     4501 tcgcgatcac cgccgacggc gccgagatcc tgtcgaacca gttgccgcgc gatccggacg
     4561 cggtggaggc ctggatcgcg gggatttggg cgcgctgatt cttgatcggc tgacccgatc
     4621 attcgcagaa cgggacaaac ctgccgctag tcgcaaccag agacgggttg gcggcgacag
     4681 cccccaacgg acggcaggtc tgtcccattt ggcccacccg ttacccgaat gggacagacc
     4741 tcgcgccagt ggcgtggtcg tgggcgattc cgcgggttag gaccagccaa tggcaggaat
     4801 gtcccgaact ggagcgcggg ccctagagtt tccgttgccc tgcggggcgt ttccaggtga
     4861 gcagagaagg agcaggcgtg tccgatccga agcagatgaa ggtcgttctc gatgaaagcc
     4921 agatgcccac gcactggtac aacatcgttg ccgacctgcc gaccccgccg cccccgcacc
     4981 tgcaccccgg cacccagcag cccctggttc ccgaagacct cgctgcgctg ttcccgatgg
     5041 gcctgatcga acaggaagct tccaccgagc gctggatcga gatcccgcag gaagtgcatg
     5101 acatctaccg gctgtggcgg ccgtcgccgt tctaccgggc gcaccggctg gagcgggcca
     5161 tcggtaccac cgcgcgcatc tactacaagt acgaaggcgc gtccccggtc ggcagccaca
     5221 agaccaactc ggcagtgccg caggcctact tcaacaagct cgccggtcgc tcccgcctga
     5281 ccaccgagac cggcgccggc cagtggggtt cggcgctcgc gtttgcctgc caggtgttcg
     5341 gcctgagctg cgacatctgg caggtgcgct caagctatga gggcaagccg taccggcacg
     5401 tgctgatgga gaccttcggc gcttcggttg tcgcgagccc gtcggagttg accgaggccg
     5461 gactagcact gcgcgagcag ttcccggaca ccaccggctc gctcggcatg gctatctcgg
     5521 aggccgtcga agcggccgcg aaggatgaga acgccagcta ctcgctcggt tcggtgctga
     5581 accacgtcgc tctgcaccag accgtgatcg gccaggaagc cctgctgcag ctgaagctgt
     5641 tcggcgagcg tccggccgac tacctgttcg cgtgcgcggg cggtggctcc aacctggccg
     5701 ggatcagctt cccgttcatc cgggagaacc ttgagggccg ctccgagacc aagatcgtgg
     5761 cttgcgagcc gaaggccgcg ccttcgctca ccgagggcga gtacaagtac gacttcggcg
     5821 acactgctca cctcacgccg ctgctgaaga tgtacagcct cgggccggac ttcgtccccg
     5881 acccggtcca cgccggcggc ctgcgctacc acggcatgtc cccgctggtc agccactgct
     5941 accacgaggg cctgctggac gcgatcgcgg tcaagcagtt ggacgctttc caggccggcg
     6001 tgctgttcgc ccgagccgag ggcatcgtgc ccgcctcgga gtcgaaccac gccatcgccg
     6061 gcgccatcgc gcaggcgcgc gctgcgaccg aggccggtga gtcgccggtc atcgtgatcg
     6121 gcgtctccgg ttcgggccag ctcgacctgc cggcctacgc cgagttcctc gccggcaata
     6181 tggccgacag ctgaattcca gcctcaggca ccgccctcaa ccaacaaggt ggactgaaat
     6241 cgtcgtggtc gacgagttca gtccaccttg tgcgtgttgc ggctactcgg ccggacggtt
     6301 cggcttcgtc tcgccgggct gggtcggctc agcgggcatg ggcgcgctct gttgcggcac
     6361 cgcgcccacg tccggcgccc cgtactggcc cgggccggac gggtggtatc cgcccggcgg
     6421 ctgcgggtag ggctggggct ggtagggctg ctgcgggtag ggctggccgc cgtactgctg
     6481 cgggtacggc tgcccgccgt aggggtgctg gccgtatcca ggcccttgtg ggtagccgac
     6541 cggttcgtat cccggctgcg gcggagcggt ctgcgcgctg gcctgagcac cggccgggtt
     6601 gaacgcggcg gccctggcgc cgggcatgtt ggcgatcagc tcgcgggcct ggccggacac
     6661 cttgtgctcg gccaggagct cgtacttggt ggcgacggtc tgcgagaccg aggtgaagtc
     6721 acgcttgccc tggctcatcc agtacatcag cgcggccatc acgatgccga ccacgatgcc
     6781 gatcaccagc gcgtaagcgg tcatcagcag tacgttctgg ctcggcatga acagccacat
     6841 cagcagcgcg atcatgatgc cggtggagat gccgctctgc acgccctgac cgatcaccgt
     6901 cccccagctg cgccgcccga gcacgcgttc gatcgagcgc agatctgtcc cgacgatgca
     6961 aaggttctcc accgggaatc gggaatcggc gaggtggtcg accacctgtt gggcctcgtc
     7021 gtaggtgttg aagacaccca ccgactgcgg atactccagc ttgaacaggc tgctcatctt
     7081 cacggctggc tggctcatcg gctctcctcc attccggcgg cccgcccgcg ggccaggatt
     7141 gcggcagcca gcttaacgcc ggcctcatcg accatctcat cgtccaccac gatcgcgccc
     7201 accgctccgc cggccaatgc ggtggcttcg gcgtaggcgg cgctaattcg ctgcgcgcgt
     7261 tcgaagtcgg cggctttggg cgagtagatc tcgttgcccg cgtccacctg tgccgggtgt
     7321 agtacccatt tcccgtcgta gcccagagcg gcgctcaact cggccgaacg ccggaagccg
     7381 ggcacgtccc ggatcgcgac gaacgggccg tcgatcgcct gcaacccgtg cgcgcgggcg
     7441 gccacgagga tcgacatcag cacatggtgg aaggcgtccg ccggataccc gtccggttgg
     7501 gcgccgacgt tcagcccgcc catgccgagg ctcgccatga agtcgcccgg accgaagacc
     7561 aggctggcca gccggggact ggccgctgcg atctcgttca catgcaggag gccgactgcg
     7621 tcctcgatct gcacctcgag cgagatccgg cccaccggca ggcccgcgtt gtgctcaacc
     7681 tgggtgagca gcaggtcaag cgcctgcacc tgggcggccg actgggtctt cggcagcacc
     7741 agcgcgtcga tccgcgcgcc ggccgaagag accacctcga tcaggtcacc gtaggtccac
     7801 gggctctccc agccgttcac gcggacggtg accagaccgg ccgcgaagcc ggcaccgctg
     7861 ttcagcgcag cggcgatccg ctgccgcgac tcgaccttca cggccggcgc gacggcgtcc
     7921 tccagatcga ggaagagcgc gtccacagcc agcgtccggg ccttctcgat gaagcggtcg
     7981 gacgaacccg ggacggccag gatcgtgcgg cggggcgcgg cgcggttgat ggtcacaggc
     8041 cccagcctat tgcagccctc agacagccgg ctcgaagagc tgggtcaccg tcagccgagc
     8101 ccgtggccga acgcgaacag cgggtcgtcg aagcctggct ggtcctccgg gtgtgtgcgg
     8161 acatcggcca tcgacctggg caggtcgaac ggcagccggc cgcggggcgg cacccgcccg
     8221 gtcaccgcat cgaagtaggc gccgccgccg gtgccgtagc tcaccgtgat cgcggcagcc
     8281 acgtccagca gcggcgtcag caccgccgga cggtcgcagg tcacgtccac cagcagcggg
     8341 caatgcgccg cgatccgggc cagccgcacg atcaatccgg gtggaaactc cagcgaaccc
     8401 tggtggaacc aggcctccag gaacaggtcc gagcgtgact cgaacggcgc cggcaaccga
     8461 acgatcgcga cgtcggccga atccggcgtt tccacaacct catagtcgag gccgacctcg
     8521 tcggctgcca gccccaagcc tagatccgca gccccccggc gagcggcagc aagcctgcgt
     8581 ttgtcaggac ggtcaccgac tccgactggg cccgaagccc ggccgccagg tagtcgccac
     8641 gtccgaccac ctgctcagcg acatcctcgt ccacgaatgg gttgtcgaac aggccgaggt
     8701 cgaacttgac cgccagcagc cggcgcacgg actcgtcgag cctggcctct gggacgcggc
     8761 ccgactcgaa cagcccgagc agcagctcga cgcattcctc gccgccgaac tggtcggcgc
     8821 cagcttcgag gatgcgctcc atccgtccgg acgggtcgag gtgctcgact ccccacgccc
     8881 gcgccggcag cacctggtca ccgacgtggt tgtcgttgac gagctcccag tcggtgcaga
     8941 tcacgccttc gaagccgagc cgttcgcgga gcagcccggt caggatctgc cggttgtagc
     9001 cgaagccgac cggctcgatc gcctcgccgt cgatctccag cccgatcggc atgccgtagt
     9061 agggcgtgac cgcggacgtt ccggctgcga tcagtgccgg gaacggacgc aggtggtcgg
     9121 cgaagtgccc ggacgggtag acctgctcgc gtccgtacgg gaagtgcgcg tcttcgccgc
     9181 cggcctgcgg gccgccgccg ggaaaatgct tggtggtgca ggcgacgctg gtgcgtccga
     9241 ccggaccctg ctgcaggcct tccagctagg ccagcgacag ctcggtgacc agctcgggat
     9301 cctggccgaa ggcgccggcg atccgcgccc aacgtggctc ggtggccagg tccacggtcg
     9361 ggtgcagcgc agcgcgaatc cccagcgccc ggtattcggc gttcacgatc tcggcgaacc
     9421 ggcgaaccag ctcaggatcg tgcagcgcgg ccagcccgat cggctccggc cattggggca
     9481 gcccgcgggc cgtgaacgaa gccccggcgt tctcgaagaa cgagtgcctg gggtcggtgg
     9541 agacggtcac cgggatgccg tgcggggtca gctcggccag caactgcagg ttggcgtggg
     9601 cctgacgagg ctcgtagagc acggccagga aggacgcgtg cggggtggcc accccgtccc
     9661 cgaacgtcgg tgtcgggttc acgcccgtcc ggcaggtgcc gaagccgtcg tccacgttgg
     9721 tgctttcctg ctcggagaag tagccctccg gattgagccc gagcgcgtcc acgccgtact
     9781 ggcggtagcc accggccggg ttcgaggacg gcgagaatcc ccagatgccg tagccggcct
     9841 ccagtagccc gtgctcacgc tgggcgcgca cgtgcagcgg atggttcact ctccaggagc
     9901 ggggcgccca cttcgcctcg ggcacgaaca cgtccggcat caattcctca aacctgctgc
     9961 cgccccagcc aggcacgatc cgcatgcctc gatagcgata ggcgccctcg taagcgtcca
    10021 gcccgaggta ggtacgggtc tcccccaccg gctgcgattc gggccaggac cagtcgcagg
    10081 tctccgggaa cgtgcgccag agcgcgaagt actgcttggc cggagtctga ccggtgaaga
    10141 tgcctaggta ggtggtgatc cgggtctagg agacggtggt gccaccgcgg tctccaattg
    10201 cttgccgaag gtggccagct tctgatcgga ctgcagcgcc gggtcctgcc aggccgctgt
    10261 gacggacggg aggtcagtgg aaagctggta ccacttcacc tgggtctcgg gctggccgag
    10321 ccagtcgacc agttacggtc tgccaggaat cggtgccgcc gggctgcaaa ttgatgcccc
    10381 acttggcgcc cgccttgtcc cgtatcgcct tggccatggc ctccagcccg tcccagtcgg
    10441 tcggcggcgt gctgaagccg gccttctcgg cgaggtcggt gcggtagtag accagcctgg
    10501 tctccacatg ccacgggata ccgaacgacg tgccgcccac ctcggtggtg gcctgtgcgc
    10561 cggctaagaa cttgctcttg tcgatggacg cgggcgtcgg ttcgagcgca tccaagccgg
    10621 cgaactcacc catgcacgtg gtgccgatca tggccacgtc cggcgtcttc tgcgcgctga
    10681 tcgcactggt gaacttgtca tgcgccgcgt cccacggaat cggggtgacg gtgatcttga
    10741 cgtcgggatt tgccgcctgg aagtccttga gcaggtcggg cagcttctcg ccctccgccc
    10801 catcgcccac acggtcagtt cgcccgtggc cgggccggaa gccaccgttc cggccggttc
    10861 cggggccgcg ctcgggttgt ccgcggcccg tccgcaaccc gcgagtgcga ccgccagtac
    10921 tgcggcgatg gctgctgccg gtgcccaacg gctaccggac cgcctatgct cgaatctcat
    10981 cgttcctcct cgaaccggcg tccgccaccc cgtgcggggc agccggacgc tcgtgaggag
    11041 gaatacccac gaccctggcc ttgctcactt gactaggcac ttctgtgcgt accgcaccgt
    11101 ggcggcagcg ggttcagact ggagtgaccc cgagcttgaa acccaccaga tttcgcttgc
    11161 ggccggctag ccgatcggcg agcgcgagca gggctttcgc tgctggccgc tccggcccgg
    11221 tggctaccaa gggcacgccg agatcgccac ccgcggcgag ttgcgggtcg atcggtatct
    11281 cggcaagcag cggcacctcg tagccgagcc gggtgctgag cgtcccggca acctcgctgc
    11341 cacccccgct gccgaagacg tccacgcggt ggttctggcc gcagtgcggg caggcgaggt
    11401 cgaggaacgc catgttctcg accacgccga tcaccttctg ctccagcatc gaggccatcg
    11461 tgccggcccg ttcggcaacc tctgccgatg cctgctgcgg ggtggtcacc acgagcacct
    11521 cggcgttggg cagcttctga ccgagcgaca gcgcgacatc gccggtgccg gggggcaggt
    11581 ccaccaccag gaagtcgagg tcgccccagt acacgtcggc caggaactgt tgcaacgccc
    11641 ggtcgagaat cgggccgcgc caggcgatca cctggtcgcg gctgctcttg agcatgccga
    11701 tagagatcac ccgcaggccc atcgaaggca ccggcatgat catgccgtcc accgaggtcg
    11761 gggacgcgtc catcaggccg agcatctgcg gcacggagtg tccgtagatg tccgcgtcca
    11821 gcacgccgac gcttttgccc ttcgccgcga gcgccatcgc caggttcacc gtgaccgagc
    11881 tcttgcccac gccgcccttg ccggaggcga ccaggatcac ctgggtcagc gaatcaggct
    11941 tgctgaacgg gatctcgcgg tcgtcgtgac cacgcagcgt ccgcttcagc tcggcgcgct
    12001 gctcgtcgct catggtgcca agcgtgaggt cgaccgcgct tacgcccggt accgacagca
    12061 ccgcttcgtt gacgtcggcc tcgatcgtgg aggctagcgg gcagccggcg atggtcagca
    12121 acacggtgag cgcgaccctg ccgtcatcgg ccacatcgac ggactgcacc atgcccagat
    12181 cggtgatcgg gcgccggatc tccgggtcga tcaccttcgc cagagcagcc tgaacttcat
    12241 caacaagcga ggacatgacc gccacgatag ccaccgagcg tccgcggtgc ccaaccggcg
    12301 gcgccccgtc cgcggatcgg gctgcggcga atgggacaaa cctgccgcgc gtcggcggtt
    12361 cttggccgta accgtcgact tgatgcgaat ggcggcagct ttgtcccaat ccttagggcg
    12421 cagcccgttg cggcggcgcg cgcgggcgcg atcagctcgg cttggacttt gcttcggctt
    12481 cctcgcgctc gttcagctcg accatcaggt cgcgcagttc agagcggatg aagtcacgag
    12541 tggccacgtc attcatccgc attcgcaggg atgcgatctc gcgcgccagg aagtccatgt
    12601 cggcgcggga ctgagccgcc acctcacggt cgtgctcggc actgacccgg tcccttgcct
    12661 cctgccgatt ctgggcgagc aggatcagcg gtgccgcgta ggaggcctgc agcgacagcg
    12721 caagggtcag gaactggaag ggccaacgat cgggcgcatc cggtaccacc aggttccagg
    12781 tgagccacgc gatgatgaac accgacatcc acaagatgaa ctggccggtg cccatataac
    12841 gcgccgcgct ctcggcgaag ctgccgaaag cgtcatcctc cagccgcatg cgcgggatcc
    12901 agccgctgcg gcgccccggc gtatccagcc gatcctgctt agccatcgct cacctccagc
    12961 cggtcgagct gtaccccgcg ccagtcgctg gggaggatgt ggtcgagcac gtcgtccacg
    13021 gttaccgccc caagcaggcg acgctcggtg tcgaccaccg gcgcgcacac caggtcgtag
    13081 gtggcgaagt agcggctcac ctgggcgata ttgctctgtg gtgtcagcgg ggtgatgtcg
    13141 ccgtcgatca gccctgccgc cagcacggac ggcggttcgc gcagcagccg ctggatgtgc
    13201 accgcaccga ggtagcggcc ggtcggggtg tccagcggcg accggcagat gaacgccagc
    13261 gctgccatcg ccggcgtgat ctcctgacga cggacggccg cgagcgcgtc cgccaccgtg
    13321 gcgtcggcgt tcaggatgac cggttcgggg ttcatcagac cgccggcggt ggccgcgtcg
    13381 taggtcagca acgtgcggac gtccttggcg tcctcgggct ccatctgctc aagaatcgtc
    13441 tcggccaact cggaatccag ctcagcgatc aggtcggcag cgtcgtcggg atccatctcc
    13501 tccaggacgt cggcggcacg ctcggtatcc agcgaagcga tcacctcgac ctgctcgtcc
    13561 tccggcagtt cctccagcgc ctcggccaga cggtcgtcgt ccatcgcctg cacgacggcc
    13621 gcccgcagca gcgggtccat gtcgtggagt tcgcgagcca cgtccgcagg cttcaactca
    13681 ctcagccgca tcacctgctg ctcggcgtcc cgcggcgccg gcttctcgat gaagtcgggc
    13741 acctcggacc agtcgacgat ggtcacgtgc ccgcgctgga acggacgccg gctgctctcg
    13801 cgcagcgcga cctcggccag cacccagtcg tggtgccggt tctgcttgat cgccacgtcg
    13861 aagatggttt ccggctggct ggcaccggcg cgggtcaccg tccggtcgaa cagcgcgtcg
    13921 acgaccagca cctcgcgctc ccgcacctgg aaacggaccg tgttcaccag gccggagatg
    13981 atcacttggt tggcatcgat cgatttcacc cgctccatcg ggaagaagat ccgccgcagg
    14041 gcgaataact cgaccactag ccccttcacc cggggcggac gttttccaga acggttctgg
    14101 atgacgacat cacgaacctt gcccacctgc tcgccgttgg ggtcgagcat gggcagtccc
    14161 ttgatcctcg aaacgaagat cgacgaagct ggcgtcacgc ttggcaggtt atcgcctccg
    14221 gcggattgaa cccaccccgc ggactcaggt ggcttcggtg tcgaacgcga ccgcgagcgc
    14281 cagccgagct ggcactgccg aagtggcgac cacttccggc gcaggggcga cggtcgcgat
    14341 cggctcccgt cctgaagcgc ttccagcggt ggtcgtggct gccaaggtgg ccggagattg
    14401 agcggcgccg gtcagggtgc tcctgatcgg gcccaactcg gccttcaccg gatccagttc
    14461 ggcctgcacc gggttgagga tgtgcttgtt gaccagcgcc ttcggactca ggtcggcgag
    14521 actcaggttc tcgaactccg ggcccagttc cttgccgatc tggcccttcg catcattggc
    14581 gatgttgcgg acgtagttga gcacccggcc ggtcttgcgg gcgagctcag ggagcttctc
    14641 cggcccgaag aggatcactg cgaacacgac caagaccagg atctcggaca tgtcgaagtt
    14701 gaacggcatg tcctaaaggg tatcgctgga accgagaacc gggttggtca gagcccggcg
    14761 gcatcgagga tggccacgaa ggctgcagtc tgttccgcgg atgcatccag cagtggcttg
    14821 cgtaccggcc cgggcgcgaa gccgcgggcg gccagtcccg ccttgaccaa catgcagccc
    14881 tgggtggcga acacaccgag gtagacgggg agcagcgctt gatagcgggc aaccgcgcca
    14941 gcgacgtcac cggcagcgta ggccccgatc atcgccttgc tggcctgccc ggtgaagtgg
    15001 gtggacgtgc ccaccaagcc gacaccgcca accgccagca gcggcagggt catcgcgtcg
    15061 tcaccggagt agtaggccag atcggtggca gcgatcacct gcgccgatgc gaccggctgt
    15121 cccttggcgt ccttcacggc cacgatgttc ggatgcgcag cgatctggat catcgtcgcc
    15181 gtctcgaccg gaacgccggc gcgatgcggg atgtcgtaga tcatgatcgg cagcgtggtc
    15241 gcgtccgcca cggtgaggaa gtggtcggcg agcgcgtcct gcggcggccg gctgtagtaa
    15301 ggggtgacca cgagcaggcc gtgggctccg gcccgttcgg cctgccgggc cagttcaagg
    15361 gtgtgatggg tgtcgaaggt gcccacgccg gcgaggatcc gagcgcggtc gcccacctct
    15421 tccagcaccg cttcgaggac agccagcttc tccgcgtccg tcgtggtcgg ggactcaccg
    15481 gtggtgccgt tgatcaccaa ggcgtcgttg ccctgaacgt cgaccagatg tgcggccagc
    15541 cgcttcgcct ccgacaggtt cagcgaacca tcggagttga acggggtgac catggccgtc
    15601 tggaggcgac cgaaaggcag cgcagtcatg ggggcaagcc taaagggtgg tgatcacgac
    15661 catggggtcg ccgtctcgaa ccacgtcgcc gacccgaacc acgatctcgc tcacagtgcc
    15721 cgcgacgtcg gcgagaacgg ggatctccat cttcatggat tccagcacga ccaccgggtc
    15781 ggtggcctgc acctgctgcc ctgccacaac ctccaccgaa aggacgttgg cgaccaactc
    15841 cgcactcacc acatggctca tgggacgcaa tgctagaggt aagccttcgc acaagtccgt
    15901 ttccacccgc atcatctgga catggcttcg ctaggctgag gccgtgaatc catcaccgaa
    15961 gaccccgggt cgcgcttcga cccttccggc cacgatcgcc tttgccgaag ccttcgcccc
    16021 ggagtcggac gcggccgagt ccgcacgcga gcgcgcgaaa caactcggcc tggagtgcat
    16081 cagttcggga gcggcgacca cgctgacctt cctggcgact gcggtcgggg cgaagacggt
    16141 ggtggagatc gggaccggga ccggcgtatc ggctctggcg ctgctcgccg gcatggccgc
    16201 cgacggcgtg ctgacgagca tcgacggcga ggcggagcat caactggctg cccgcgaggt
    16261 gctcgccgca ggcggggtgg ccacgcgtcg cgttcgcttg atcgcggggc aaccgctcaa
    16321 cgtgctgccg aagctgaccg acggcgccta cgacctggtg ctcatcgacg gtgatccgct
    16381 ggagtatgtc gagtacgtcg cacaggcgga gaagctgctg cgttcgggcg gacttctggc
    16441 actccatcac gcgttgtggc atggcctggt cgccgacgag cgcaatgacg acgacgagcc
    16501 gctgatcatc cgcgaagctc tggccgcggt gtccacgaac gaggccttca caccagccct
    16561 gttgccgatc ggcgacggcc tcctggtcgc ggtcaaacgc tgaagctcgg gtcacgccga
    16621 gcggtaacgc ccaccacagg attccggaac gggacaaacc tgcccgcagc ggcggcgtaa
    16681 cccggtttga agccgatacc ggctcgctgg ccacaggaat gtcccggaag ctgccctacc
    16741 cacgcacaga cgacacagcg ggccgcccgg cagactgccg gacggcccgc tgcgaacctt
    16801 aggtgcgggt gatcaccagt tgcctggcac ggccatgttc ttggccacca cggtgatacc
    16861 gccttcgacg tggaagccgc gtgcgcggtc gtggtctggg ttcacaccga tctcaacccc
    16921 gtcgggcacg atcacgttct tatcgaggat cgccctacgg atgaccgcgt tgcgcccgat
    16981 ccggcagccg gagtagatca ccgactcctc gatcttcgcc cacttgtcga ccatgacgtt
    17041 gggcgaaagc accgagcgat ccacgtcgcc tccggaaatg atgcagcccg accccacgat
    17101 cgaatcctca gcggtgccac gcagggtgaa cttggcgccc ggcagctgcg cctgcgacgt
    17161 ccagatcggc cacttgcggt tgtacaggtt gaactcgggc tcgaccgaga ccagatccat
    17221 gtgcgccgcg tggtaggcct caatggtgcc cacgtcccgc cagtagttgc ggtccttctc
    17281 cgtggaaccg ggaacgttgt tgagggtgaa gtcgtagacc tgtgcgcgtc cggacgagac
    17341 gaaggccgga atgatgtttc cgcccatgtc atgcctggaa tcggcgaccg cggcgtcgtc
    17401 gttgagcgcc tccacgaagg ccttccgggt gaacacgtag ttgcccatgg acgcgaacga
    17461 ctcgtccggc gaatcgggca gacccggcgg gtcggccggc ttctccaaga aggcgttgat
    17521 cttgccgtcg ggggcggcgt cgatgatgcc gaaggcactg gcctcgcgcc gcgggacgcg
    17581 gatgccggcc acgctcgcgt ccatgcctga atcgatgtgg gctttcacca tgtcctcgac
    17641 gttcatccgg tagatgttgt cggcgccgaa gaccaccacg tagtcggggt cctcgtcgtt
    17701 gatcaggttc atcgactggt agatcgcgtc cgcgctgccc tggtaccact gcttgccggt
    17761 gcgctgctgc gcggggacgg acgtgacgta gctgcccagc agcaccgaca tccgccaggt
    17821 cacggagatg tgccggtcga gcgaatgcga cttgtactgg gtcagcacac agaccttggt
    17881 caggtcggag ttcaccaggt tggagagcac gaagtcgatc aaccggtagg tgccgccgaa
    17941 gggaacggcc ggcttggccc ggtcggcggt cagtggcatc agccgcttgc cctcgccgcc
    18001 agcgagaaca atcgaaagaa cgtttggtcc actagtcatg gcaccgaacc taggacctcc
    18061 gcagacctac cacaagaaaa cagctcaaaa cgcacgcgtg ttgctcgatg aatcctccga
    18121 acggatcatc cgtaacccat ttcggggcct cgcgttgtgt gacacgatcg cgctatggca
    18181 cttcgagtat cgatccttac ccgcgagttc ccgccgcaca tctacggcgg cgcgggcgtc
    18241 cacgtggggc aattggtgcc ccaactgcgc aagttcaccg agagcgtcga ggtgcaatgc
    18301 ctgggcgaac cccgtgaggg tgcgaccgca catgccgagg acttcccacc gggcgccaat
    18361 gccgcgctgc gggtgctcgg cgcggacgtg tcgatggcca acgcgatcgg cgacgtggac
    18421 gtgatccact cccacacctg gtatgccaac ttcgccggcc tgatcggctc gaagttcagg
    18481 gacgtccccc atgtggtgac ctcgcacagc ctcgagccgc accgcccgtg gaaggccgag
    18541 cagctcggcg gcggttaccg ggtctcatcc tgggcggaga agaccgcctt cgaggacgca
    18601 gcagcggtga tcgcggtcag cgccggcatg cggggtgacg tgctcgcctc ctacaccgat
    18661 ctcgacccgg accgcgtcca tgtggtcaag aacggcatcg accccgacga gttcaagccc
    18721 gaccagggga cagacgtggt ggacgcgctc ggcgtggacc gggaccgtcc gttggtggtc
    18781 ttcgttggcc ggatcacccg gcagaagggc ctggtacacc tcgtccgtgc cgccaaggcg
    18841 ttcgaccccg acacccagct gctgctgctg gccggagcgc cggacacccc agagatcgcc
    18901 gccgaattcg agggtgcttt cgctgagctc agcaaggccc ggtccggcaa cgtgaaatgg
    18961 gtgcaggaga tgctgccacg agcctccgtc cggcaggtgc tcacccacgc caccgtgttc
    19021 gcctgcccgt cgatctacga gccgctgggc atcgtcaacc tggaagccat ggcctgtgag
    19081 acggccgtgg tcgcgtccgc ggtgggcggg attcccgagg tggtggtgga cggcaagacc
    19141 ggcaccctcg tggcctacga cccgaagcaa gcgggcgatg ccgagtatgt ggccaacttc
    19201 gaggcccagt tcgccgagca ggtcaaccgg atcacccgtg accctgcgtt ggcgaggagg
    19261 tatggcgtgg ccgggcgcca gcgctgcatc gacgagttct cgtgggagaa gatcgcgcag
    19321 gagaccgtag cggtctacga gcgggcgatc gcctatcacc agtcgaagtg acctggactc
    19381 ccgggcgcga acccgggagg aggccagaaa tgcgaagagg ccccgggatc accggggcct
    19441 cttcgcaact ggtcacgagt caggctacga cgacgccctc cagggcagtc ttcaactcag
    19501 ccgcttcggt ggcgttcatc tcgatcacca accgaccgcc accctcggca gggatgcgca
    19561 ttgcgatcac acgaccctcc ttggcgacct cgatcggccc gtcaccggtc cgcggtttca
    19621 tcgctgccat cacatacctc aatttctggc tgttcggatc caactccctc attattccat
    19681 ccccggacgc ctgctgcggc accggcgccc cggaagggac ggttcaggcg gacggagtgt
    19741 tcgaaggcgc ttcggacgcc gggggtgctg gcggatctac gggcacggcc tgcgcgcgct
    19801 caagctggcc ggcgagccgt tcgagcacct cgtccacctg ctgcatcgag tagccacgcg
    19861 gcaccacggc gaagcggagg ccacgcaggt cggcggcagt gagatccccc gttggaacgt
    19921 gcggtgtcgg ggtgtcggtg accgtggcag ggagttcgcc cagccgtcca ctggcggcca
    19981 ccgcggctag gccgaggatc gccaccgcga tcagccagat gaacaactcc atgccggcca
    20041 gcctacccag tggtgtccct tgagtggctc accgggtcag caacccggct gaggaacaca
    20101 ccattggtca ggacatgtgc gccgaagact ccggacgcgc gttcggcccg ggtccggtga
    20161 ccagcgccac ggcttcctcc gggtcgtcgg tcacctgggt cagttgcagg tcggttgggg
    20221 cgatgtagcc cgattccagc acctggtcct gcagccagcc gagcaggggc gtccagaagc
    20281 tgctgccgaa gagcacgatc gggaatgacg tgacctttcg ggtctggacc agggtgagcg
    20341 cctcgaacac ctcgtcgaag gtgccgaaac cgccgggcat gaccacgaac ccctgtgaat
    20401 acttcaggaa catcaccttg cgcgcgaaga agtaccggaa gttgattccc agcgagacgt
    20461 acttgttcag cgactgctcg aagggcagtt cgatgcccaa gccgacggag actcccccgg
    20521 cctccagagc acccttgttc gccgcctcca tgacgcccgg accgcccccc gtgatcgtgg
    20581 cgtagccggc ctcagcgagg ccgcgtccaa tcctggaggc cgcggtgtac atcgggtcgc
    20641 gcctcggggt tcgggccgac ccgaacacgc cgatgcccgg gcccacctcg gcgagcgtgc
    20701 cgaaaccctc gacgaactcg gcctggatac gcatcacccg ccacggatcg gcgtgcgccc
    20761 aggcgccgtc acccttgctg gtaagcaggc gctggtcagt ggtcgtcggg aggacctggt
    20821 cgtggcgccg gatcaccgga ccctgcgtgc ggatctgctt gctcacacga tcaatctagt
    20881 ggcgacctcc gcttccgccg acgggcgagc accgcgcgaa tcctggcgat gacccgctcc
    20941 gggtcggcca tcagcattgc ccaggtgatg tgcagcaccg tccaatccgc ctcggtgagg
    21001 tcgttgttgc gatgtcgatc agcctcgaaa gccgcgcggc tgctgtggaa ctcgtagcca
    21061 tccacttcga gggccagttt ccgctcacgg aacgcaacgt cgatctcgta gttggcgccg
    21121 cacgcgacca ccgcgtcgtt ggccacccaa ccacgcatgt ggtggcggtc cagaaggtcg
    21181 tgcaggatcc gctccgccct ggagaacggc cgggtcttcg agcggttcac gacccgacgt
    21241 cggacacggt tgccggcgcg tttcgggctc gcggccagtg cagcttcgaa ctctgccggc
    21301 ggataaccct tgcgaagcag gaggtcgatc cggtcgccga ggtccgtggc ggcagagtcg
    21361 acggcaacct gagcggcaca gagcagtgtc accgggccac ggtgccggag ttgctgcggt
    21421 gcgactcgtc tgcggatgaa gcggtacccg tgcttggcta cgcgggttgt cgccgtaatt
    21481 gcctcgatcg tcgccacctt ttcgttcggc caagcggtca gcttcgccgc ggctgcgccg
    21541 accacgatcg cgtccggaac tctggcgacg agcgcccgca agcgagtgac caggtcgtcc
    21601 gccgcgtcgg gatgcacata gacgccgggc aggactcgca cgatgcggcc ggccaggcac
    21661 cagcggctca ggctcgactt gcgagcccgg tgttggctgg cgagcaggat gccttcatgg
    21721 tcggcgagta gtgcggctag atcgatcacg cccatacctt cggcaactcg cctcgaaatc
    21781 tgtcacgcga tctgcccatc tgtggacaac ttcggccctg tggaaaatcg ttcagctcgc
    21841 cggtttggga caactccgcc gccagcggcc gcggatcgcc agatccgggc ggatcagcgc
    21901 taatagcggc agatttgtcc cggagtcggc cagtgggcgg caccggttga tgggccgagc
    21961 agtcggcgga tcagcccagc cgcggtaagc cgggccacct gatcaccgtc ggtgccggcg
    22021 atcgctgcgg cgcttgccgc gctggctcag aatcactgcg gcgcggcggg ctcagcccag
    22081 ccagcgcagc agtgcgtccc tgcacgtgta gaggtctgcc accggtgtga actcatcgtc
    22141 ggcatgcgcc ttgcccggat ccgccggacc gaagttcacc gcaggcacac ctagcgcagc
    22201 gaagcgggcc acgtcggtcc agccgtattt ggcggacggt tccccgccca ccgcggccag
    22261 aaactcctgc gcgatcggac ggcccaagcc tggccgcgca cccagcgcga aatcgatgaa
    22321 ctcgagccgg tagccctcga acaggtccag gcagtgctgc ttcgcgtccg cctcatcgcg
    22381 gtcgggagcg aagcggaagt tgaccgtgac tgaggccgcg tccgggatca cattgccggc
    22441 gatcccgcca ctcacggcca ccgcgttcag cccctcccga taggtgagtc cgtccaccac
    22501 cacctgccgg ggctggtacc ccgccaaccg gcgcagcggc tcggccagtt cgtgaatggc
    22561 gttgtgccca agccatgccc gagccgaatg cgcggccgtc cctgcagtcc gcagaacgaa
    22621 tcgaagggtg ccctggcagc caccctcgat cctcgccgaa gtgggctcca tcagcaccgc
    22681 gaagtcggcg gccagccatt cgggatgctc tcgtgcgacc cgggtgagcg agttgtcctc
    22741 ggcggccacc tcttcatggt cgtagaagat ccaggtgatg tcgcgcgacg gtgccgccag
    22801 cgcagcggcg caggcaagcg cgacggccac gccgcccttc atgtcgcagg tgccgcggcc
    22861 gtagacccgg tcaccgacca ccgtcgaagg gagattccca gccaccggca cggtgtccag
    22921 gtgcccagcg atgaccaccc gctcggcgcg gccgaggtcg gtgcgcgcga cgacggtgtc
    22981 gccgtcgcgg acgaccgcaa ggtgcggctg cccccgcagc gcggcctcga ccaggtcagc
    23041 cagcggaccc tcgttgccgc tgacggactc gacatccacc aactgccgca gcagcgtcac
    23101 gaggtctgtg gtcagatcca aggtcatggc cctagagcct aagcccgcag ccacaccagc
    23161 gtcgcgaacc agccggcgcc ggactgcgga agggccgcta gtggttggcc gcgtccggtt
    23221 gcaccaccct gcgtccggac cgcaatttca ccccggtagg cttgcgcgca tgacccgacc
    23281 cgcgcacggc tggggcctgg ccagcgtcca cgcctccggc caggtactcg acacctggtt
    23341 cccgaatcca accctgggtg cctcgaccac caatgaaccg ccgaagcagc tggtggctgc
    23401 ggcgctcgtc gatgacgagg tgcgcggcgt ccgcctcgat ccggtgctca tcgagatcga
    23461 cctggacgca ccgccggccg gtgtcagcga cgcttacctg cggctgcacc tgctcagcgc
    23521 gcggatctgt gcaccacgca cgatcaacct gactggcgtc ttcgcagtgc tgcccaacgt
    23581 cgtctggacc aactacggtc cgtgcgcggt ggagggattc gaggacgtcc ggctcggcct
    23641 gttgggcagc cgcggacggg tgagcgtttt cggcgtggac aaattccccc gcatgaccga
    23701 ttacgtcgtt ccgagtggcg tgcgcatcgc ggacgcagat cgcgtccggc tgggtgcgca
    23761 tctggcatcc ggcactaccg tgatgcacga gggcttcgtg aatttcaacg ccgggacact
    23821 gggttcgtcc atggtggagg gccggatctc ggccggagtg gtcgtgggcg acggctccga
    23881 catcggcggc ggcgcgtcca tcatgggcac cctgtctgga ggtggccagg agcagatcac
    23941 gatcggtgaa cgctgcctcc tgggtgccga gtccggcgtc gggatctcgc tcggcgacga
    24001 ttgcgtggtc gaagcgggcc tgtacgtcac ggccggcacg aaggtgacct tgtccagcgg
    24061 cgcggtggtg aaggcggctc aactgagcgg tcagaacggc ctgcagttct ggcggaactc
    24121 ggtcagcggt acggtcgagg cgcgcacgcg ggctggcacc ggagtcgagt tgaactcggt
    24181 cctgcacacc aattgaggaa ccgatgaagg gactgcggtc gagccttttt gcggttctgg
    24241 gcgcggtcgt cgtgctggcg atcatcgcgg gcggggtcgt gctctggtcg acctacaaca
    24301 aggcaccgct tccgatcgat gaccgctgcg tggctatcgc cggcggcaac accgcgacca
    24361 tcgaccctgc tcaggcgcac tacgcgtcga tcatcgcggg catgacgatc aagcgcgacc
    24421 tggcgccgcg agccgcgtcc atcgcgttgg cgaccgcata ccaggagtcg gggattcgca
    24481 atctcgatta cgggcatgcc gactcgattg ggctgttcca gcaacgccca tcgaagggct
    24541 ggggcacgat cgaagaaatc atggacccct ggtactcggc ccgcgagttc taccgggcga
    24601 tggagcgcat cgacgattgg gaaaacaagg acatcaacga cgtcgcgcag gccgtgcagc
    24661 gcagcgccta cccggacgcg taccgccgcc acgaaaccaa cgcacgtacg ctggcgagct
    24721 cccttaccgg ggagactccg gcgtccttcc gatgccaggt cgctgcgccg aatactgccg
    24781 atcccgacgg gatgggccga ctgctcacca agacactcgg cgccgacaat gtcagcatca
    24841 aggctgtcgg cggcgagttg accgtgacgg ctgcgaacgc cactcatgct tgggcagtag
    24901 ccaacatcgc ggtggcgaac accgccgagt tcgggctggc ggccgtgcag ctgggtgagt
    24961 attcctggac ccattccagc cggaccctgg ccgagtgggt gggaaccggg cccaagtccg
    25021 ataccgaggt gaagctgcag ttcgccgtgc cgacgccgcg ctgagcggga cgaagtcggc
    25081 cacactctcg gctagcccga gaacctctga gcggccgccg ggagtgctcc cgggtttcgg
    25141 gacaaagctg gcgccagttg ctcagatcgg gccgctaacg cccggcttcg ccgactcggg
    25201 gcaggtttgt cccgaaagtt ctaggcgcgg agcgggacgt agcctgcatc cgcgaccgtc
    25261 cgggcgccgg cttcgctggt cagccactca agcaccctgc gggcagggct gccggcgggc
    25321 tcatcagcac ggaccacagc gaagaagtcg ttggcgtagg ggtagctgcc atcggcgatc
    25381 gtttccttgg tcggctgcac cccgccgacg cccagcatct tgatgccgtc gaccgcatac
    25441 atgttcgcga ggtagtagta cacgctgtag ccgagcgcat tcccggtgtt ggcgtagctg
    25501 gccacaccgt cgatgatctc gcccatctcc gcggtgatcc gatcagtcgg cgccttcgtg
    25561 agcttcgcct gacccacgac atacttgcgc agcagggcct gcgaaccgga ctcctccgcg
    25621 cgctggtaac cgacgatctt cgcgttcgca ccgcccacct gctgccagtt actgatcttg
    25681 cccgtgtaga tatcgcggta ctgcttggtg gtcagcgatt cgaccgggtt gctggagttg
    25741 gtgaagaaga ccagagcatc gcgtccgatc ggatgggctt cgagcttgac cccggacgat
    25801 tcgacctcgg ccttgaccgc ctcgtcggct tcgtagacca ctagcagatc caccgcaccg
    25861 gcgacgaggt tgcgccaggc tgcgggcgtc ccgttggtgt tcacggcgtt gtcagcatcg
    25921 gtttcggaga caccggtcag ccgctggatg gccagcgaga tcaaggggat gttcgccgtc
    25981 gagccgtcga tcgtgggcat gtccgcgagg gtgaacgaga agtccgcctg cgcgctggcg
    26041 ctctcggtac tcgcgctcgg tgtggtcgac ccggacggga ccatcggcga agtcaccgag
    26101 gcggacgggg tcggcccgga agccacgcac ccggacagga cgaccgccaa cgcggtggcc
    26161 gcgaacagga aatgacgcat tctcaatcct ccaattgggt gaaacggctc tcccgatagc
    26221 gccaggcccc ggtcgcgtcc actaagccct gataccggcc ggcgaccgcc cagtaatact
    26281 ccggaacgct gccggaagcc gccgccagag ctctaacgct gcggaacgtg gttcggttgc
    26341 cccgggtgtc gaaaacgtcc gggggcaggg tgaccacgga cggcgcgcac atcacccagg
    26401 cggtaccgat cagatcgcag aaactccaac ctgcggacgt gacgctgcgc ccgtcggcca
    26461 ggaagtggag cctggtcagg ttccgttgga gatcgtcgtc ggtattcggg tcggtaaccg
    26521 agtcgctcac aatgtcccgg ctgatcgcct tggtgagtcc ggccggcaga tcggtgcgag
    26581 tcagctcggg cagcagcacc agcttgccga gcccgtccgc cgacaccgta gtgcaggcgc
    26641 cggcaaggtt gcagtagatc ggggacgggc tgaccggcag gatcacgttc gcgtccggtc
    26701 cgagcagccc ctcctgctct ccgcgaaaca cgtggtagcc gagcagcgca tcgttggcgt
    26761 ccgtttcaac cgtgatgctg gtccagggcc cgctcacggg gttgaagtcc gcatcgatca
    26821 gggtctcgga cgtgccgtcg ccgatgatcg cgcgcccggc cacgaacggg ttggcgtcgg
    26881 cgaaccgcgg ctccacgagc cactgcccgg caaggctgac gtacccgaag gtcgaagtgg
    26941 actggtagtc cggacgcttc gctgcagccg gcagccccgg cgcgcccggc tccagcgcgg
    27001 agtaccacag ccagcccgga tgcggcgtcc gtgcgccact gctcaggtcg aggaagtact
    27061 ctccctcggg gtcggagacg ttgacgatct cgccctgcac cacctcgatg tgccgatcct
    27121 tggccagcgg cacgacgatc cggccagtcg agagctgcag taccccggag ttgtattcac
    27181 cggtctctcc ggagatcaca tcggtgaaga cgacgtgatc ggaaccggcg cagcctgcgt
    27241 ccgctgccgg cgtccgcagc atcagtttgc cggtgaggtc gagcacttcg gcgcgatgat
    27301 cgccgcgttc cccgatgacg aaggcagcgc gcccgtgggc gtccgggcag tactggtagt
    27361 tgtcgaagcg cggcttcacg acgagctggc cgttcacgtc gacgaagccg taacgctcgt
    27421 acgcctggct gccgtagtcg gtgaccaccc ggagttcgat ggtgggccag agcatcgggt
    27481 cgccgtccgc ggccggcatg gctgaggaac cgcggtattc gccggggatg gtggacgatg
    27541 ccggcgcgga cggttcggcg gacgtcccgc ccgttgcgct cggagcaatc gaagttgcgg
    27601 tcggtgggct ggtgatggcc ggaccggtag caacgcagcc ggccagagtc agcagcgtta
    27661 tcatcacgga cacccttcgc acagggtcat catgggcctc aaccggccgg acggaagtgg
    27721 tccgcgccga atctgatgag tttctcagga agcggtcggc gaagggcttg gcctcgaaag
    27781 acccgaccag cggacggaat tagccggccg cggccaacaa tcgcttggcc gctgcggcaa
    27841 cacgttcgtc cgtagcggtg agcgccaccc ggacgaactc gcgtccggcg tcgccgtaga
    27901 agtctccggg tgcgaccagg atgccgaggc cggccaggtg ttccacgctg gcccggcaat
    27961 cctcgtcgcg ggtgcaccag aggtagagcg agccctcgga atgctccacc cggtagccgg
    28021 ctgccgccag cgccggacgc agtcgggccc ggcgacgctg gtagcgcgcg cgctgctcct
    28081 cgacgtgggc gtggtcaccc agcagactga ccatcgcggc ctggacgggc gtgggcacca
    28141 tcagtccggc gtgcttacgc acctcgcgca actcgctgac caggatgggg tcgccggcga
    28201 ggaaacccgc gcggtaaccc gccgcgttcg aacgcttcga gagcgaatgc acagcaatca
    28261 ggttcgccag cgagccgcca ttgatctctg gatgcagcac cgatatcggc tcggcgtccc
    28321 agccgaactc ggcgtagcac tcatccgacg caagcaccgc accgacgccg cgcgccgcgg
    28381 ccacccactc tcgaaggcgc tgcggggtga ggatctggcc gtggggattg gccggggagt
    28441 tgatccagat caggctcggc ttgagtgcgg cgacctcggc cgggtcgtcg caggccacga
    28501 ccttggcccc ggcgatgcgt ccgccgacat cgtaggtggg gtacgcggtg gtcgggatga
    28561 ccaccgtccg accagcgccg acgcccagca gcgtgggtag gagcgcgacc aactcctttg
    28621 tgccgaccac aggcaggacg ccgtcgtcga ccagcccccg cgcgctccag cgcccggcca
    28681 ggtagtcgat gatggcccgg cgcagcgcgg gggtgcccca cgtggtcggg tagcccggcg
    28741 cgtccgccgt gtcggcgagc acgcgttggg cgatttccgg cgtcgggtcg accggcgtgc
    28801 cgaccgagag gtcgacgatg ccgtccgggt gcgcggccgc tcgggcccgg gccgcgtcca
    28861 gcgaatccca ggggaagtcg ggcagccgcc cggccagcct gctcacactg cctgcggggg
    28921 cagagcggcg acggtgggat ggtcggcgtc aatcggaccc agcttggccg cgccgccggg
    28981 cgaaccgatc tcgtcgaaga agtgcacgtt gatgtcgtag tactcggcct gatcggacgg
    29041 gacgtcgtcc tcgtagaaga tcgcctcagt cgggcagacc ggctcgcagg caccgcagtc
    29101 gacgcactcg tcggggtgaa tgtagagcat gcggttgccc tcgtagatac agtcgaccgg
    29161 acactcctcg acgcacgcga gatccttcac atcgacacat ggaagagtga tcacgtacgt
    29221 catgaaaccg gctcctagct agacagttgg cctactcgga gcactctaca accgacatga
    29281 tgagatgagg cggagtgggc ggcgcgcttc cagaacccgg tcgtcgggtg agcgtgcggc
    29341 gggtgcgtcc ggacgggtac ttcagcgacc tgatcgggtt cgtggtggca gccagcgctg
    29401 ctgagctgcg attgcaggat cggcgtggcg aaacccatca ggtgccctgg gcgcaggtgc
    29461 acgcgtggcg tccggtcggt gtggccagag gtcgcgaccc gatgcggacg ccgctggccg
    29521 aactcgatcg gcaggcccgg ctcgccggat tgtccggacg ctactacgtc gcccggctga
    29581 gcgatctgct tgactcccgg gaacccatgc cgggcagtcc gccgagtgca ccgccaccat
    29641 ctccggctga gcatgtcggc gaatgggtgg tggcagcacc gactgccgac ctgatcgcgc
    29701 tcgcgtggtg ggccgcgcat cgtgacgctc gctcattgct gctggttgcg ggcgatccag
    29761 ctgaccatga gcggctactg agcctgggca tggtggcgcg actggactga cctttcggga
    29821 caaatctgcc ccgagccagc gggtctgggc cgcgaaacgt gatccggacg ccgcgggtgt
    29881 cagctttgtc ccgcaatggt gggacgtcgg cgctgagcag ggtctcgaca gagttggacc
    29941 agccgggccg gcgcggcggg ctcagtcctt cttggccggg tccttgccgc agaccactgg
    30001 ggtgagggag ttgtagctcc acttgaatgg ctccttcttc aagagcttct cgccctcgta
    30061 gaagagccgg tagaacttca cgtcgaagcc gctcatcgcg ctctgcgcga tgcagccatc
    30121 ggagtcgtcg tagacggtct tgccgggcgc gcggtagttc gaacgcaccg gcgtggtcga
    30181 cttgatcgtg taccgcttgg tcgaccaaac cttgaccgtg accgagccct gactaccggt
    30241 cttgccggtg atcgaagcct gcagcagcac cccgtaaggg gaatcgttct tgaacttcat
    30301 gtcgacgctc tcccagtcca gcgtggcctc gcggccgacc gggtagcggt tgaagtacag
    30361 cgagtgcggc ttgtggtaga tgtccttcag cccggcgaaa tagatcgcgt tgaaggtggt
    30421 cgtggtggcc tgcgagatgc cgccgcccag gcgctcgacg atcttgccgc cgtcaattgc
    30481 gccaccggcc atccaccctg cggcggtggt ccgcttgccc agggtctggt tcatgctgaa
    30541 ggtctcaccc ggcagcagga acgtgccgtt gatcagcttg gccgccttgc cgatgttgtt
    30601 gtagcggtaa gccgtgccag ggaagtaggt ggtgaacttg ccgatcacct tcttgacccc
    30661 gagctgctcc acatcggccg tggttaccgc cgcaggttgc gcgctgacct caacggacgc
    30721 gctgcgcccg gacgcctggg agagcaccgg caccagagct gcacccaact gctcgggatc
    30781 aactccgaca ccgtcgacgg acgggacgac cttcggcttg cccttgctga tcgtgtagcc
    30841 ggcgttcttc ggctgcttca agccgagccc ggacagctgc ttggcgacct ggctggcaag
    30901 ggttttcgag tcgaaggaag gcaccagtga tccgtccttg ggctcgaact tgaggctggc
    30961 tgcgatcgcg gccggctgga ggttcagctt gcccttgtcc gcaacgctga cagtcaccgc
    31021 ctcacctatc gcgctcttcg ccaagccgtc agcgacatcc tgagcctcgg tggtggtgac
    31081 ggcaggttcc gcggtggcca acggcgcggt caccggctca gccttgaggt aggcggcctg
    31141 gatcgcagcc ctggtggcgg cccgatccac ggccacgccg gccttggcat cgctgaccac
    31201 cggtttgagt ccggaatagg cgacgccggc gttcaccggt tcgacatcca ccttcgcggc
    31261 gagcgcgtcc acggctgcgt tcagcttggc ctggtcgacc ttgagcaccg cagcctgctc
    31321 ggcgccaccg aacaagacgg tcaacaggtg caccgggttc cacgaggagc cgccgccggc
    31381 ctgcgccacc gaggcttcgt agtccacggc gagcccggcc tcggcaggct tcagcgacat
    31441 cgattcgtcg ccggccttca ctgaaatcgg cgcagcggac gcggcggcca gctccttctc
    31501 cagcttcgcg acagcctgcg cggagttgag cccgccgagt tggacgccgt tcaccgatga
    31561 cttgaccgga aggttctcac cggcaaggaa gtgtccggcc acgtaaccgc caccgagcag
    31621 cgcgatcagg ccggcgccta cgccgatacc gatctttgcg gttgtggaca gccccttgga
    31681 tggtggcaac tggaattcct ctcggttcgg gcacccccaa tgttacgtgt acgaagtaag
    31741 cggctcggga tcggctgacc gggcagaagg cgtccccaac tgcgcgacca cgatcccgat
    31801 gatcatcagg gccgcaccgg cgtagccgcg cagccccatg aactcgctca gcagcagcgc
    31861 cccacccagc gcgccgaaca ccgattcggt ggccatgatc agggaggcat gggtggccag
    31921 ggcgtgccgc tgaccgacca cctgcagggt gtaggcgatg cccaccgaca cgagcccgcc
    31981 atagagcagc gggatcgccg cctcgacgag cccaccgaag ggtgccgact cggcaaggag
    32041 cgcgacgacc gaactaacca cagcgcaagt gaggaattgc accagcgcga accgcagcgc
    32101 cgacaatcgt cccgcgaagt gctcgatcac gaggatctgg agcgcccaga acacggtgga
    32161 gaggatcagc agccactcgc cgcgtccgat ggtcagctcg tcgttgacgc tgatcaggta
    32221 cagccccacc agcgaagcca cgacgccgag cacggtggcg aggttgctgc gatgcccacg
    32281 gaaggcggcg atcaccggca cgaacaccat gtacagcccg gtgatgaacg ccgcgttccc
    32341 cgccggggtg tcggacatcg ccacctgctg caggttcacc gcgatcgtca tcagcacgcc
    32401 ggacgcgatg ccgggcagca ccactgcgcg ggtggccgcc gtccgtttgg ccctcggcac
    32461 accgcggacg cgatcgcgca cccagatgat cggcagcagc accagcgcac ccagcgcgaa
    32521 gcggactgcg gtgaagctga acggcccgac gtgcgccgcg ccgacccgct gggcaacgaa
    32581 ggcgaacccc cacagcgcgg acgccaacag cagcagcccg ttggcggtga gccgttgtct
    32641 cgggctgctg ctgagttgtc cgggttgagg cagcggtcag cccagccggg agagggcggt
    32701 gcgggcgcgc tcggcgacgt tcgcggcagt gaagccgtac tttgcgaaca gcacaccggc
    32761 ggctgccgac tcaccgaaat ggtcgatggc gacgatctcg ccggcatcac cgacgtactc
    32821 gcgccagccc atcggggagc ccgcttcgac ggagaccttg gccaccccga cgggcagtac
    32881 ggacgccctg tactcggcag actgggcgct gaaccactcc tggcagggca tcgagacgac
    32941 gcgcgtgccg acaccctcgg cctgcagcag gtcacgggcg gccagggcca cctcgacctc
    33001 ggaaccggtg gcgatcagga cgaccttcag ttcgccgtcg gcgtcgacca gggtgtaggc
    33061 gcccttcgtc agttcggacg cagccgcaac cccgggctgg tcgcggtcga ccaccagcag
    33121 atcctggcgg gaaaggatga tcgcgctcgg ccggcccgaa ttggccagcg cagcggccca
    33181 ggcctgggcg gtctcgttgg catcggccgg acggatcacg tccagccccg ggatcgcccg
    33241 cagcgaggcg agatgctcga tcggctggtg ggtggggccg tcctcgccga cgccgaccga
    33301 gtcgtgcgac cagacgaaga tcgaaggcac cttctgcagc gcggccagcc ggaccgcccc
    33361 acgcatgtag tcggcgaaga ccaggaaggt gccgccgtag cagcgggtca ggccggactg
    33421 ggtgatcgcg ttcacgatgt tgcccatggc gtgctcacgg atgccgaagt gcagcgtccg
    33481 gccggtgtag tcgcccttga agtcgtgcga ggactgctcc ggcggcagga aggacagctg
    33541 gcccttcatc gtggtgttgt tggaaccggc gaggtcggcc gagccgcccc acagctccgg
    33601 caccacatcg gccagcgcgg tcagcacctc accggacgcg gcgcgggtcg actttttgcc
    33661 cggcgcgaac actggcagcg caccctgcac gtccggcagg ccacgtacca ccagccggtc
    33721 gaacaggtcc gcctgcgccg gcgcagccgc gcgccaggct tcgaacttgg gcgtccactc
    33781 ggccttcgcc gcagcggccc gatcggcagc ctgcttccgg gtgtaggcca gtacctcggc
    33841 ctcgactgcg aagttcgcgg ccgggtcgaa gccgagctgg gtcttcagcg cggccacctc
    33901 gtcagcaccc agcttggcgc cgtggacgga gtggtggccg gccttggtcg gcaacggcca
    33961 gccgatcacc gtggtcaact tgatgaacga cggcttgtcg gtgaccgcct tggcggcctc
    34021 gatcgcgtcg tggagggcct gcacgttttc ggtgtagctg gtgccaccac cggtccagtc
    34081 cacgtgttgg gtgtgccagc cgtaggcggc gtatcgcgcc gaaacgtcct cggtgaacgc
    34141 gatctgggtc tcgccctcga tcgagatccg gttgtcgtcg tagaacagga tcaggttgcc
    34201 cagcttctgc gcgccggcca gcgacgacgc ctcgccggag acgccctcct gcatgcagcc
    34261 gtcaccggcg atgcagtaga tcgtccgatc gaagatcgag gactccccca gcggggtggc
    34321 cggatcgagc agcgcccgtt cgcggcgagc ggccatcgcg aagcccaccg cgtccgccac
    34381 gcccgcgccg agcgggccgg tggtgcactc gacgccggcg gtgtgcccga actccgggtg
    34441 gccgggcgtg agggagccct cggtgcgcag cgcggcgagg tcgtccacct caacgccgta
    34501 gccagccagg aacaactggc agtactgcgt caacgacgag tggccgatgc tcagcacgaa
    34561 gcggtcccgt cccacccact tcggatcagc cggatcgtgt cgcatcacct tctgatagag
    34621 cagataggcc agcggtgcca tcgagatcgc cgtgcccggg tgaccgttgc ccaccttctc
    34681 cacagcgtcg gcggcgagga ttcgcgcggt gtcgaccgcg cgctcgtcca actcggtcca
    34741 ggtgaactcg gtcatcatcg gcctttcggt tgctttgaaa cgtaacaata ctgactgcgc
    34801 gcttgcaggt tccagctttt cacaccactc gggttcggcc gatcggggcc catttgttgc
    34861 atcgctaagg caactacaga ttcccgaatc acttcgtaag caaataccct ggccggtatg
    34921 gtggtaggta gtcctactta caggagttca acgatgaaac gcctggtcat cctcggtggc
    34981 ggcacagccg gcacgatgat cgccaacaag ctgcgagccg ccctcggcaa gcacgaactg
    35041 cacataacgg tggtggatcg cgacgatgcc caccactacc agcccggata cctgttcatg
    35101 ccttttggca tctacaacac gtccgacatc gtccgctcgc ggcacaagtt catctcggac
    35161 ggggtcgacc tcgtacttac cgagatcgat cgcgtcgacc ccgcctaccg ggtggttcac
    35221 ctcgcggacg gacggacgct gagctacgac gtgctggtga tcgccaccgg cgtcaccccg
    35281 cgtccggacg ccaccccggg catgcagggc aagctgtggg gcgagaaggt gcatgagttc
    35341 tacagcctgc ccggcgccca ggcgctgacc gacgcaatgc gccggttccg cggcggacgg
    35401 ctggtggtgc acatcaccga gatgccgatc aagtgccccg tggcgccgat ggagttcacc
    35461 ttcctggccg acgactggct gcgcaagcac ggcattcgcg agcgcaccga ggttgtctat
    35521 gtgacgccgc tggacggcgc gttcaccaag ccggtggcct cccgcgaact cgccacagcg
    35581 ctgaccgacc ggcagatcac gctggagacc gacttcgcgg tcgaatcgat cgacaacgac
    35641 gcggccgaac tggtttcgtt cgacggccgc cggatcggct tcgacctgct ggtcaccgtg
    35701 ccgctgaaca tgggcgcgga ctacatcgcc cgctccgaac tcggcgacga gctgaacctg
    35761 gtgccgtgcg accaacacac catggtgagc ctggcgcacc cggagatcta cgtgctcggc
    35821 gatgccggca cgctgcagac ctcgaaggcc ggatcggtcg cgcacttcgc agtggacgtg
    35881 ttcgtgcaca acttcctggc cgaatatcgc ggcgaaccgg ccacccacgg cttcgacggt
    35941 cacgcgaact gcttcatcga atcaggtaac cgcaagggca tgttgctcga cttcaactac
    36001 accaccgagc cgttcacggg aaacttcccg ctgccggtgg ttggcccgtt caagctgctc
    36061 aaggaaaccc atctcaacca cctcggcaag ctggcgttcc gctggatcta ctggaacatg
    36121 ctgctgcccg gacggccgat cccgatgatc ccgacgaaca tgagccttgc cggcaagcgg
    36181 gtggagaaag aggtcagcgc cgaagccacg gggctgccgt ccatcggggc gccgtccagc
    36241 ccgccgccac cgcgtcctgc ggctccggct gcgaagccaa agcttgaggg acgcagcgcg
    36301 ccagcgcgcc ccaaagccgc cccactggcc aagccgaagg gcgccgcgac cccgtcccag
    36361 gcaaagccga agccagcacc cagcggcggc tacgttgacc gcccgggggt ggactcgacc
    36421 gctgacccgg ccaccgtcct gcccaacccg atcatcccga agttctgagg agcgagatgc
    36481 caaccaccct gatcgccggt cgtccgatcg acgtcaacga cgagggtttc ctggtcgcct
    36541 acgaccagtg ggacgagaag cttggcggtg aactcgcccg gctgatcggc ctggagttga
    36601 ccgatgcgca ctgggcagtg atccggttcc tgcgggccga ctactcgctg cgcagcgaga
    36661 ccgcgacgct gcggcgcgcc agcacggtag ggggcttccc gatcaaggaa ctgttcgaac
    36721 tcttcccggg caagcctgcc aagaagatgt cctatatctc cgggctgccc aagcccaagg
    36781 gctgcgtgtg agcgcggaag gagccgtacc aatgaccgac gcaccaatcg tccccgactt
    36841 cagcggaccg gactgccccg ccccgaacgc cgcaccggcg cagcggaagc tggtgatcat
    36901 ctgctcgaag ggcaacctgg acatggccta cccggggctg attctcggct cggccgcgct
    36961 gggtgagggc atcgagaccc acctgttctt caccttctgg ggtttcgaca tgatcaccaa
    37021 gggacgcatg gacgacctga agttcacgat gctcggcaac actgccatgc acctgactca
    37081 ggcgccgggc atgccgatcc cggcgatgct cggcaccctg cccggcatga ccaagatggc
    37141 caccaagatg atgcagaagc agatcgccga cctggagatt ccgacggtcc cagagatgct
    37201 ggaacagatc cacgagatgg gcggccacat gtgggcctgc cggatgtcgt tcgacatgat
    37261 gcggctcaac gaggccgacc tctacgaagg tgtggaggcc gtgatcagcg cgaccgactt
    37321 catggagttg tcggagggcg cgcagatcat cttcatctga ggtcttggcc tcgacgtgct
    37381 gaccagccgg ttggtcagca tgtcgagaag ctcaagcgaa ctgcgcgccc gcctgctcgg
    37441 tcaccgcagc cacgagttcc ttgatccgct cggcgtcctc agcgcgggcg accagggtgg
    37501 catgcggcgt ccgcaccacg acgaggccgc tcacgccgag caggccgatc accgagccct
    37561 cgtcagcgtt gacgatcacg ttgtcgttgg cgccaagagc gacgctgaga ccggaaatgg
    37621 cgttgccgga cgcgtccgtg gccaggatct cggccaggct ggcgtagcca ccgacatcgc
    37681 gccaactgat cggcagcgcg accgccacga catgcgcgct cccctttcct ccggaaaccg
    37741 gttccatcac gccgtaatcg atcgaggtct tcgcaagtcc agggaagatc tccgccagcc
    37801 ggtcaggatg ctcggccaac tcgaggaccg aggcgtgcgt ggccggcagt agttgccgca
    37861 actggtcgag cagggtggtc gcccgccaga cgaacatgcc ggcgttccac cagtactcac
    37921 ctgacgccag ataggtctcg gccaactcga tcggcggctt ctcgacgaac cgcagcacgc
    37981 gatgcgcgcc cgggaaaccg tcgatcgcct cgcccctttg caggtagccg tagccggtgt
    38041 gcgggctggt cggcaccacc ccgagcgtga ccagcgcgga ggcgtccgat tcggcgaggg
    38101 caaacgcggt ctccagacac tccgcaaaaa ccttgggcgg ctcgatcacc tggtcggcgg
    38161 tcacctgggc gatgacggcc tcgggatcct gccgggccag cacggctgcg ggccaggcga
    38221 ccgcgttcag cgagtcgcgt ccgatcggct cgccgaggat gttgcctgcc ggcacctcgg
    38281 gcaggtcggc ggccacgacg tccgcgtaag ccgcccccgt gaccacccac acctgctcgg
    38341 cgggcacgga cgccagcgcc cgctcgaacg ccagccgcag caggctcttg ccctgaacca
    38401 gcgggagcaa ctgcttcggc gtgccttgcc gcgaaagcgg ccagagtcgg gtgccggatc
    38461 ccccagccat gatcaccacg tagcgcatgg ctggagctta cggcctcggt ccgctggcac
    38521 agaccaggat tgtgggacag agctggcgcc agcggccggc caacgtcggt cggggcagcc
    38581 gcggcacgac tggcggcggt tttgtcccga aatggtcggg ctggtcggcc cgtgggccgg
    38641 tgcggcagca tggtgaaatg gagaacccga tcatcgccgc gctcaccgac cgcgtccggc
    38701 gcgccggctc ggagccgttg ctcacctggt atcggccggc cgacgggcag cgcaccgagt
    38761 tcagcgtgaa gagctttgcg aactgggtcg acaagaccgc gaacctgctc gacaccctcg
    38821 gggtggaagg cacggtcgcc ggatcgctga acaccagcca tcctggtcac tggatgagcc
    38881 tggtctggcc gttggcggcc tggcagcacg gcattgccta cgcggtcggc agctccgacc
    38941 aggcagagct gatcgtcgtc ggcccggagg acccacagcc cagccctggc gcgctcacga
    39001 tcgcctgttc gttgcacccc ctgggcctcg gactatgcaa cctcccggac ggcgtgctcg
    39061 acttcaccac cgaggccctc gccgagccgg acgcgcattg ggcggctccg gtcgtagaga
    39121 cgaaccgggc gtgggcggac gccggactcg cccgcaccca cgcccaactc gccgacctgc
    39181 cgcccgagcc cacccgcgtc ctagtgcgtc cgaccacacc gtgggccacg ctggctgcgg
    39241 ccctggtcag cccgctgctg ggtggcggct ccgccgtcgt ggtggacggc gcggccagcg
    39301 aggccgacct ggcccgcatc gcggctaccg agcgggcccc ccgctcaatg gcatgatggc
    39361 tgcgatcaca cgcgccgacc agggatatcc catgacttcg atgagccgcc gctcactgct
    39421 cgcctccgct gccgccctcg ccgggggcct ggcgatcgcc gggtgcgcgc cgagcattcc
    39481 gaaaccgtcc gcatcgccaa ccggtccgat caccagccaa ctcgacgaag tgctgaaggt
    39541 cgtctccggc ggcagcgaca agttcggcgt gttcgttcgg gacgtgcgct ccggtggcac
    39601 ctacagcttc aacggcgact acgccagcca gaacgcttcg atggtgaagg tgatgatcgc
    39661 gctgatggcg cagcggcagg cgcgcgcgga cgacagcacc ctgagccagg agaacaccga
    39721 tctggtcacg aaaatgatca cccactccga caacgacgcc gcagaacagc tctggcccta
    39781 cgccggtggc gcggacgcct accagcagct cgcggacgag ctgcgaatgt cccatacgca
    39841 ccgcgacccg aaccgtccgg actggagctg gacctggacc accccgtccg atcaggtgca
    39901 gctgctggac tacctggtca cgggcggcac cgacgcgttc accgcggccg agggcaagtt
    39961 cgtgtacgac ctgatgggcc aggtacagga cgatcagacc tggggcgtgg gtgcgccgaa
    40021 gtccggcacg gtgcaggtgc acctgaagaa cggctgggtg cagttcaagt cgagcgacgg
    40081 gctgtgggcg gtgaactcga tgggtacggt gcagggcgag ggccgcgact accgcatggc
    40141 ggtgatggcc cgggtcgccg atttcgacac tggtcgtgag ctcacctccg aagtcggccg
    40201 ccaggtgttc tcgatcctgg gttccggcag cctctgacct caggcccgct tgagccggcc
    40261 gaacttgtgg agacccctgc tcgcgtcgcc tcaagaaccg ggacaggtct gccgccagtg
    40321 acacgaactc gcgccgaccg tcccgattcc gacgactggc gaccggtttg tcccattcgg
    40381 caccggcgtg ggctcagtcg gcgaagtggc gcggtcgacg aaccgcgacc gatctaggcg
    40441 ctcaccttgc agaccgcatc gaggttgtcg gtctgggtcg atgacggctt cttcttcggg
    40501 gtgggcgagg ccgaacccga ggcggaactc gaagcggtcg actcgggatg agcgtccaga
    40561 gcctcgctat cagcaatctt ctcggtcacc acggcccgga ttttggcgag gtccgggtcc
    40621 atcggcacga tcagtggcgg ggtgaagctc accgaggcca tcggcatcga cttgcccttc
    40681 atggccaggt cgagcgccgt cccgatctcc ccggagggca ggttggtcgc cactatctgc
    40741 tcgccggccg cggcgatgtc ctggaacttg gtcagcaccg tggtcgggtt gagctgcttg
    40801 agcatggcgt tcatcacgca cttctgccga atcatccgct cgtagtcggt ggagtactcc
    40861 cgcgagcgag cgaaccagag cgcgtggaac ccgtccagct tgacgttctt gccgggcttg
    40921 atccacccgt agacgccctt gggtccatgc aggctgccga tcggcacctt cttgccgatg
    40981 tcgagccgga tgccgccgac cgcatcgatc agttgctgga agcccttcag gtcgatcatg
    41041 gcccagtagt tgatcttcag gccgagggtc tcccccacca ccgcggcagt ggtctcagcg
    41101 ccggggtact tgacccccgg gaacagcttc ttgttctgct cggcaagcgt gtacacgccg
    41161 ttgagcaggc aggcgtccgc cagatcctcg acgtggcaga agtagccgtt ggggtacttc
    41221 gcgtgcagcg gcgagtcgga cgggaagggc gcgtgctgca ggttgcgcgg caggctgaac
    41281 agcactgtcc ggccggtatc ggcgtcgatg ctggcaaccg tcatcgagtc cggacgcagg
    41341 ccctgccggt cagcaccggc gtcgccgccc atcagcagga tgttgatccg gccgttctcg
    41401 actttgctgc tgccgccgcc ggtgaagatg ttcgtgacca gcttcgactg ggcggcgaag
    41461 agtccggaga ccgtccagga caccgcccca ccggtggcca tcagcgcgag cgccacaccg
    41521 ccggagacca gcttgccggc cgtggacatt gcgcggggat tggccaacaa ccaggcgtcg
    41581 agcaccagca ggatccagcc gagcccgagc gccagcacca gccagcgcag caccgtcagg
    41641 gtgatcggat tggcgtagag accgatgatc tgggtgcgga acgggatcag caggagcagc
    41701 gcgaccacca gaacggcgag cagggtgagc cagacccgca gcgcgacgcg gccgacgttt
    41761 cgcttgccga ccgcgagttg ggccgagccg gggaccagta ctgacatgcc cagcagggtg
    41821 agtccgcggc gcagggcgat gccctggctg cgtcgctgtg gggatgctag aactgagccg
    41881 gcgggctgag cggtggaagc catcagtctc ctgcgagcgt cgggatcaag gcatcctcca
    41941 gtattaccga gccctcccaa gcggtggtcg aggcacgccg cccttcgtca ggttcaggca
    42001 gcgttag
//
LOCUS       DAOPRQ010000011        41767 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_709_length_41767_cov_11.501966, whole genome shotgun sequence.
ACCESSION   DAOPRQ010000011 DAOPRQ010000000
VERSION     DAOPRQ010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 41767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41767
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_709_length_41767_cov_11.501966"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..895
                     /locus_tag="PKJ61_02395"
     CDS             <1..895
                     /locus_tag="PKJ61_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004811323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HOC12711.1"
                     /translation="TENPSFSVISPLLSRSLLLTLQPLTDADIGVLLDRALTEERGLG
                     GAFTLTADARTALLRFAGGDARRALTYLEEAAAGAEAQDTSEIDAEVVGAAVDKAAVR
                     YDRDGDQHYDVISAFIKSLRGSDVQAGLHYLARMLEAGEDPRFVARRLIILASEDIGM
                     AAPSVLQTCVAAAQAVALIGMPEARINLAQAVVACALAPKSNAVIKGIDAAIADVRAG
                     RIGTVPPHLRDAHYPGAKAHGHGSGYQYAHDAPHGVAAQQYLPDELAGAKYYEPTDHG
                     NEAALAERLAMLEELLGKDNS"
     gene            948..1337
                     /locus_tag="PKJ61_02400"
     CDS             948..1337
                     /locus_tag="PKJ61_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HOC12712.1"
                     /translation="MSAGEVAGLIAAMAAVAFVVFCAVPLLKLGRVLEELRLAVRDLG
                     HNSVPILQELRGTVAATNSELGKLSLVTEDVAKVSAHATVVSENAAQLSTLFTSTLGG
                     PMIKTAAFTYGVRQAVGGRKPSKKAGK"
     gene            1337..1564
                     /locus_tag="PKJ61_02405"
     CDS             1337..1564
                     /locus_tag="PKJ61_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC12713.1"
                     /translation="MGKRLFWFAVGVGLTALVVVKGREYYERFTPKGVAEQLEKTRSS
                     ASAWLGDFFETMNEAMDEREGELREALGLVE"
     gene            1648..4296
                     /gene="alaS"
                     /locus_tag="PKJ61_02410"
     CDS             1648..4296
                     /gene="alaS"
                     /locus_tag="PKJ61_02410"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004811321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOC12714.1"
                     /translation="MKTADIRQRFLDFFAAREHTIVPSTPLVYNDPTLLFVNAGMVPF
                     KPYFTGAEDAPFKRAVSVQKCVRTLDIEEVGKTTRHGTFFQMNGNFSFGDYFKEGAIE
                     YAWELVTGSPAEGNYGFDPDKVWVTVYLDDDEAAGLWRKVGIPDERIQRRGLKDNYWH
                     MGIPGPGGPCSEIYIDRGPEFGPDGGPIVDEDRFLEIWNLVFQTEELSAVRAKDDFDV
                     ARPLPAKNIDTGMGLERTAYLLQGVANMYEIDEIFPVIKHAAELAGTTYGKDQQADIR
                     LRVVGDHVRSALMLMTDGVTPGNEARGYVLRRLLRRSIRSMRLLGVQDKVLPELLPTS
                     RDLMATSYPEITEHWARVSQVAYAEEEAFARTLAAGTQIFDLAAGQAKTEQVTEISGD
                     RAFALHDTYGFPIDLTLEMAAEQGLSVDQAGFRRLMQEQKDRAKADARAKKGATVATE
                     AYRTLRSAGEVPFLGFTDLTADATVRGIVAGGQLVNRAATGDVVEVVLDQTPFYAEAG
                     GQDADTGLITGDGATLEVLDVQRPVQGLVVHKVRVADGELASGARMVAQVDAAARAGA
                     CQAHTATHIVNATLRQLLGESTHQAGSYNKPGYLRFDFNATGALSHGLREELEGVANE
                     AIRADYEVTAQEMPLAQARALGAQAMFGEKYGDIVRMVELAGPWSRELCGGTHVKRTS
                     QIGLLSLLGEASVGSGLRRVEAFVSADAFAHLAKERALVAGLADLLKVQPDQLSDRVE
                     KLVAQVKAAEKEIAALRSRELLANVPGLVAAATSTGAYELVSKHLPGTSADDLRSLAM
                     EVRTAFGARPGVVALIGGDAKPALIVATTATARELGAKAGALVGVGAAALGGRGGGRD
                     DLAQGGGANADAAPAALAAISAALAG"
     gene            4299..4781
                     /gene="ruvX"
                     /locus_tag="PKJ61_02415"
     CDS             4299..4781
                     /gene="ruvX"
                     /locus_tag="PKJ61_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOC12715.1"
                     /translation="MRTGVRLALDWGKARIGVAASDPSGILAHPVETIPAGPDAFARV
                     QRLVAEYEPIEVVLGLPRNLAGVEGPAALAIRQVAAELVAKLGALPVRLVDERLTTVT
                     ASRQLSGAGRNTRKQRAVIDQAAAVALLEQALQYERTTGLPLGSIVEPREETRKDSSA
                     "
     gene            4778..5926
                     /gene="mltG"
                     /locus_tag="PKJ61_02420"
     CDS             4778..5926
                     /gene="mltG"
                     /locus_tag="PKJ61_02420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HOC12716.1"
                     /translation="MSNRMPGEIRDPDTGKIDYKEIWYKLRSAFAVLLSLAVLAGAGW
                     FVYDKARGAWMEFRTAEDYIGEGVAPVEVTIPEGATLGQISDILVQAKVVKTAKAFDR
                     EAEANADSKTIQPGKRMMKTELPAKVALSMLLDKNLIIRNRFTLAEGKWLAQQYPVME
                     KATGVTVKEFKAAAKDWKKLGLPTWAEHGLEGFLFPDTYEVPEKPTAKSVIKMATKQF
                     AAVAKELDLEATAEEMGRTPYQVLVMASIVEKETFKAEDRPKVARVILNRLKAGTPLG
                     MDSVIAYAVKKSGVLELTAEDLAIDSPYNTRTRKGLPPTPINSPTKSAIEAALHPAEG
                     DWTYFITVDPSSGETLFTANYDEFLAGKQKYKDWCEASKENRKVCYGE"
     gene            5931..7163
                     /gene="aroC"
                     /locus_tag="PKJ61_02425"
     CDS             5931..7163
                     /gene="aroC"
                     /locus_tag="PKJ61_02425"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016669858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HOC12717.1"
                     /translation="MLRYLTAGESHGRALVATIEGLPAHVRITTADIADALARRRLGT
                     GRGARMKFEADEVTLLTGVRHGETLGSPVAIMVGNSEWPRWETVMAPDPVDAAELEQL
                     ARNAALTRPRPGHADLAGMQKYDWDESRPILERASARETAARVALGRLAANFLEQAFG
                     IQVLSHVVRIGGAAVGDSGPSSLRHAQGEPSSGSVPLPGDLAAIDAHPVRCFDSVAGA
                     AMLAEIATAKAEGDTLGGVVEVLAYGVPAGLGSHIQWDRRLDAKLAQALMGIQAIKGV
                     EVGDGFGLAATRGSLAHDEMVPGADGVERLSNRSGGTEGGMSNGEPLRVRAAMKPIAT
                     VPRALRTIDVSTGEAAQAHHQRSDICAVPAAGVVAEAMVALVLAEVALEKFGGDSVAE
                     TRRNYEAYLSRLAERRLA"
     gene            7160..7660
                     /locus_tag="PKJ61_02430"
     CDS             7160..7660
                     /locus_tag="PKJ61_02430"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HOC12718.1"
                     /translation="MTPALALIGIPGSGKTTVGELLAAQLQLPFIDVAELVEAEVGGS
                     APEFFAGAGEAAYREIEEAVTLAALDETAVIALSSGAINSAAVRGVLGGEVVWLRTNV
                     STATRRLSMNALGMAALVAIRNRMDAMLSQRAPWYSEVATQVVDTDRLTPTEVAAAVL
                     ENQGGH"
     gene            7660..8745
                     /gene="aroB"
                     /locus_tag="PKJ61_02435"
     CDS             7660..8745
                     /gene="aroB"
                     /locus_tag="PKJ61_02435"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HOC12719.1"
                     /translation="MTWEPIDVATEQPYQVLIDSGVAKLLPQFLDGVDRVAVIHPPSL
                     ADKLAVVTAGLDQQITTIPVPDGESGKTAHVLTGCWEALAEAGFTRSDAIVGLGGGAA
                     TDLAGFVAATWLRGVKYIALPTTLLAMVDAAVGGKTGINLAAGKNLVGAFHEPFTVLG
                     DLDFLHTLPAGELRSGMAEVLKCGFIADPEILTEFEADVTAALQPASAVQAKLIRRAV
                     RVKAAVVASDLRERTSEGGNIGREALNYGHTLGHAIERSEHFRWRHGEAVAVGMVFAA
                     TLAHSAGLIDAELLARHRHVLELAGLPTRYPQGPWLELRDAMNLDKKSRGSSLRFVVL
                     GGLGMPRILQGSSEGELADAYLTMNSI"
     gene            8756..9391
                     /locus_tag="PKJ61_02440"
     CDS             8756..9391
                     /locus_tag="PKJ61_02440"
                     /inference="COORDINATES: protein motif:HMM:NF016207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HOC12720.1"
                     /translation="MYVPVHFAMSADQIADVLASAPAGDLVTDGPDGLTATYVPMLWD
                     PESGSLIGHLSRVNAQWRDAGEALFIVHGPDDYIAADWLSTPAADSVPTWNYVTVHVY
                     GTLVAHDDPDWTLAAVRRLSRAHSDPAIDDLPADEVAKLLRAVVGVELRDLRMVGKAK
                     MSQNKSPQVVGQVIDGLRDAGGVDTAAWMAEHSLPRAEAKAALLAAIRERG"
     gene            9437..10000
                     /gene="efp"
                     /locus_tag="PKJ61_02445"
     CDS             9437..10000
                     /gene="efp"
                     /locus_tag="PKJ61_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOC12721.1"
                     /translation="MATISTNDLKNGVVLDIDGQLWTVIWFQHHKPGKGNTVVRSKLK
                     HVLTGKVVDRTFNSDTKVESANVDRREMQYLYHDGEAYVFMDTDTYDQLNLSEEVVGD
                     AKNYLLENQVATVALHEGNPLYIDLPTSVELEITYTEPGLQGDRSTGGTKPATVETGL
                     QIQVPLFLTTGERVKVDTRDGSYLGRV"
     gene            10001..10432
                     /gene="nusB"
                     /locus_tag="PKJ61_02450"
     CDS             10001..10432
                     /gene="nusB"
                     /locus_tag="PKJ61_02450"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOC12722.1"
                     /translation="MTSPEPGKKLSTRTKARKRALDILFEADLRGLPATEVLAAHTAE
                     ADPPVRPFTADLVRGTFTNLRLLDTLIASHLPSGWTLERMPRVDRNLARLTAYELGHT
                     EIDTAIALAECMTLAGELSTDDSPGFLNGVLAAVARDVRPA"
     gene            10587..11804
                     /gene="carA"
                     /locus_tag="PKJ61_02455"
     CDS             10587..11804
                     /gene="carA"
                     /locus_tag="PKJ61_02455"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HOC12723.1"
                     /translation="MPSSLFSTSALPALLVLEDGRWFRGSSFGAAGESFGEAVFNTGM
                     SGYQETLTDPSYHRQVVVATAPHIGNTGWNDEDDESKRIWVAGYVVRDLSRVRSNWRS
                     NRSLDTELRNQGVVGIAEVDTRAITRHLRERGAMRVGISTVELDPEKLLAKVLQAPPM
                     AGANLVADVTVTEASVTPAVGEKRFTVAAIDLGIKDMTPQRMSERGIEVHVLPATVSV
                     AEIRELAPDGVFFSNGPGDPAAATAAVELLTEVLDAGLPYFGICFGNQIFGRALGFGT
                     YKLKYGHRGINQPVMDLTTRKVEITAHNHGFAVDAPLDRHTVTRWGKARVSHIGLNDQ
                     VVEGLELRDDRGGLLGFSVQYHPEAAAGPHDAGYLFDRFVDMMAARGGQEVAEPTEGP
                     PTLRQAQGTEEDA"
     gene            11804..15139
                     /gene="carB"
                     /locus_tag="PKJ61_02460"
     CDS             11804..15139
                     /gene="carB"
                     /locus_tag="PKJ61_02460"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012921929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HOC12724.1"
                     /translation="MPRRTDINSILVIGSGPIVIGQACEFDYSGTQACRVLREEGFRV
                     ILVNSNPATIMTDPEFADATYIEPIRPEYLEQVIATERPDALLATLGGQTALNAAVTL
                     AETGVLEKYGVELIGASIEAIQKGEDREKFKEIVASLDVPGVNPEVARSAICHTMDEV
                     LAAADQLGYPVVVRPSFTMGGVGSGFAHDETELRRIAGRGLQASPVTEVLIEESVLGW
                     KEYELEVMRDTADNVVVVCSIENLDPMGVHTGDSITVAPAMTLTDREYQAMRDVGIAV
                     IRAVGVDTGGCNIQFAIHPDNGRLIVIEMNPRVSRSSALASKATGFPIAKIAAKVAVG
                     YTLDEIPNDITAETPASFEPALDYVVVKAPRFAFEKFPTADSTLTTHMKSVGEAMAIG
                     RNFTEALGKALRSLEDAKAGFDFTGEIAESLDELLATMNRPHDGRLNVVMQAFRLGAT
                     AAQIHDATKIDPWFVDQLQLIYEVAQHVRTAPELTADVLRLAKRHGLSDGQIAKLRNL
                     STDVVRGVRWALGVRPVYKTVDTCAAEFAAHTPYHYSSYDEETEVEPRTKPAVLILGS
                     GPNRIGQGIEFDYSCVHAAMALRAAGYEAVMVNCNPETVSTDYDTSDRLYFEPLTLED
                     VLEVYHAEAQAGPVAGVIVQLGGQTPLKLAQALKDAGVPIVGTPPEAINLAEERGAFG
                     RVLADAGLPAPAYGMAESAEQALRIAHDIGYPVLVRPSYVLGGRGMEIVYDDASLASY
                     IERATDITPAHPVLVDRFLDDAIEIDVDALYDGTDLYLGGVMEHIEEAGIHSGDSACS
                     LPPITLGNEVIEQIRRSTEAIAAGVGVRGLLNIQYALLGDILYVLEANPRASRTVPFV
                     SKATDTQLAKAATRISLGATIAELRAEGMLRTGMDGTATIEAAPIAVKEAVMPFNRFR
                     TADGSWVDTLLGPEMKSTGEVMGLDLTFGTAFAKSQAASFGAVPTSGTVFVSVANRDK
                     RHAIFPVKRLADLGFKVLATEGTAQVLRRQDVDVTVIRKNSQGPGPNGEKTIVQAILD
                     GEVDLIFNTPQGVSKDGNPRRDGWEIRSAAILADVPCVTTVQGLSACVQGIEALGRGA
                     VGVRSLQEWSAGVRAELAGQDAG"
     gene            15139..16230
                     /locus_tag="PKJ61_02465"
     CDS             15139..16230
                     /locus_tag="PKJ61_02465"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016455971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HOC12725.1"
                     /translation="MGLPAGLLNAGYVELLRPLLFGSFGGDPEAIHEQMIHALAGVGS
                     AAPVRALTSLLAASQGHPVRVAGINFPGRVGLAAGMDKDASAVLAWQGLGFGHAELGT
                     VTAQAQPGNDRPRVFRLRSTGALINRMGFNNPGAAAVAATLASRNVYRGNGVAGIPIG
                     ISIGKTKVVDLADAVEDYLTSLRLLAAHADYVAVNVSSPNTPGLRSLQDAGALAELLG
                     ALVAETCLASPAAAGLPIFVKVAPDLTWCQLDEVLATAQDAGVAGLIATNTTLARDGL
                     ASADQHLADQAGGLSGSPLTVRAREVVAYLTRHTELPVIGVGGIMTPGDAMAMFDAGA
                     QLVQLYTGFIYRGPGLAAAINALDVERHR"
     gene            16230..17045
                     /gene="pyrF"
                     /locus_tag="PKJ61_02470"
     CDS             16230..17045
                     /gene="pyrF"
                     /locus_tag="PKJ61_02470"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HOC12726.1"
                     /translation="MSTYWRRLREVVAERGALCVGVDPHVSLLKAWDLEVSAAGAERC
                     ARTVVEALGDLVAVFKPQSAFFEAYGSAGIAALERVLADIRATGALSILDVKRGDIGS
                     TMEAYADAYLTEGSPLAADAITVSPYLGFGSLAPAIERAHRYERGVYVLARTSNPEGA
                     QVQLALTQDASVVQSIIDAATEVNHASRQKAIGLVVGGTHDSLGCDLEEFNGSILVPG
                     IGVQGGRLEDLPDLFGEAVDQVLPSVSRGVISAGPDASAMRAKVSALLSQAVR"
     gene            17158..17475
                     /gene="mihF"
                     /locus_tag="PKJ61_02475"
     CDS             17158..17475
                     /gene="mihF"
                     /locus_tag="PKJ61_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004811306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor, actinobacterial type"
                     /protein_id="HOC12727.1"
                     /translation="MTIPQLTTEQLEAARAAATEARRRRAELKTKVRSGILTLSQALE
                     TAAEDDVLAHVKVVDLLKALPRVGEKRAAVVMERLDIAPNRRIRGLGRHQVAGLKAEF
                     SDR"
     gene            17465..18016
                     /gene="gmk"
                     /locus_tag="PKJ61_02480"
     CDS             17465..18016
                     /gene="gmk"
                     /locus_tag="PKJ61_02480"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016667666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOC12728.1"
                     /translation="MTGDVTVISGPTAVGKGTVVKALRARHPEVWVSVSATTRPPRPG
                     EVDGEHYLFVSARGFDDLVASDGLLEWALVHGVDRYGTPAAPVQAAVAADRRVILEID
                     LQGARQVREHLPEARLVFIAPPSQETLVKRLEGRGTETPEQMARRLRTAEGELAAAAE
                     FDHVIVNDDLGSAVDELVELLGL"
     gene            18064..18402
                     /gene="rpoZ"
                     /locus_tag="PKJ61_02485"
     CDS             18064..18402
                     /gene="rpoZ"
                     /locus_tag="PKJ61_02485"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016455976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HOC12729.1"
                     /translation="MSTHIPEGITNPPIDELLTQVDSKYRLVLFAAKRARQINAYYSQ
                     LGEGLLENVGPLVDIGIQEKPLSIALREVNAGVLECTDIDPEAEAAARAEASADPDFG
                     LDPFGTDDLA"
     gene            18412..19632
                     /gene="coaBC"
                     /locus_tag="PKJ61_02490"
     CDS             18412..19632
                     /gene="coaBC"
                     /locus_tag="PKJ61_02490"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HOC12730.1"
                     /translation="MSRIVLGVAGGIAAYKACLLLRRFTEAGHDVRVVPTASALEFVG
                     ATTWAALSGHPVSTSVFDEADAVPHVRIGREADLVVVAPATADLLARAVTGRADDLLT
                     NVLLTAGCPVVMAPAMHTEMWSHPATQANVATLRSRGVVVLRPAEGRLTGADSGPGRL
                     PEPEDIEATCLAVLAQADAAGAMAAQDLRGLNVVISAGGTREALDPVRYVGNFSSGLM
                     GIALARAAVQRGADVTLVGANLHAEPPAGVRVVEVVSTADLAAAMAELAPSADVVVMA
                     AAPADYTPAAPSDTKLKKSGDAGLTVEFRQTTDVLAGLAARRPVGQIVVGFAAETARD
                     EAELIELGTAKLARKGCQLLVLNNVSDGAVFGSGENEVLLIAPVGSRGRAAGTKTTVA
                     HAILHAAIAERSAE"
     gene            19629..20816
                     /gene="metK"
                     /locus_tag="PKJ61_02495"
     CDS             19629..20816
                     /gene="metK"
                     /locus_tag="PKJ61_02495"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOC12731.1"
                     /translation="MSRLFTSESVTEGHPDKLADAISDAVLDELLSQDPTSRVAVETL
                     VTTGTVVVAGEVTTEAYADVAQLARQRILEIGYDSSEKSFDGNSCGVLVSLGVQSPDI
                     AQGVDHAEEQREEGSFDALDAQGAGDQGLMFGYACTDTATLMPLPIDIAHRLSEQLAK
                     VRRDGDLDFLLPDGKTQVTIEYAGDRPVRVDTVVVSTQHTAATTHDRISAEVRARVID
                     PVLARYAIPSSGMKTYVNPTGKFVIGGPMGDAGVTGRKIIVDSYGGMARHGGGAFSGK
                     DPSKVDRSAAYAMRWVAKNVVAAGLAERCEVQVAYAIGRAHPVGFYTECFGTEKVPVE
                     QITAAVLDVFDLRPAAIIRDLDLIRPIYSQVTNYGHFGRELEIMTWERTDRADALASA
                     VRG"
     gene            complement(20837..21190)
                     /locus_tag="PKJ61_02500"
     CDS             complement(20837..21190)
                     /locus_tag="PKJ61_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOC12732.1"
                     /translation="MRIQSVTVGLPCADIGRSLEWYRTVFGFVAPGIEPAPGVVEFKV
                     GPIWLQLIAEPVLRNGAQPVVRFGVIDAEVERERLLRLGLPAGDLQQVEGVLEYFEFS
                     DPDGNLLSMYAEVEP"
     gene            21377..23326
                     /locus_tag="PKJ61_02505"
     CDS             21377..23326
                     /locus_tag="PKJ61_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863511.1"
                     /note="binding of PriA to forked DNA starts the assembly
                     of the primosome, also possesses 3'-5' helicase activity;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosome assembly protein PriA"
                     /protein_id="HOC12733.1"
                     /translation="MKLPVARVAVDLPLANLDRPFDYLVPEALDAEAQPGVRVKVRFA
                     GQLRDGFLLGRAPVGERAKLAALQRVVSAEPVLDPGVTELVRAVADHYAGSFADVVRA
                     AVPPRHAATEKAPPPAHPEPQLADREPVTLSAYPFADRFLGALAEGGHPRAAWTVVPA
                     PVPAGDWASGLVEAAEVALSSGRGALLLVPDARQLQHLVGVATARFGRGSFATLAADA
                     GPAARYRNFLAVRRGGVRLVLGTRAAAFAPVPNLGLIALWDDGNDAYAEPHAPYWHAR
                     EVVALRAHQAGCALLLAAHSRTAEIQQLVQRGWLAELQLGPKPTRRIAAAVRTAGPEN
                     SRDPLATVARLPHDAFGVVRAGLAAGPVLVQVPRAGYLPIQVCAGCRATARCPACGQA
                     LADGSGSGLSCPLCGPLLTSWRCPDCGDTRLRRPVVGVARTAEEFGRAFPGTKVLQAS
                     ADKPLTKVGAEPALVLATPGAAPPADGGYAAAVLLDAELMLARADLRTGEESLRRWLA
                     VVSAVRPGEAGGTVIAVADPGLREVQTLLRLDPVGYAEQELAERVAAGFPPAVKLITV
                     EGPMPAVTAVLAALDLSPVVQVLGPFEVPGRCGVLDEPVARATLRCGLVEGAELVSAV
                     RAVMSVRAAKKEVPVRVRVDPQVFA"
     gene            23417..24208
                     /locus_tag="PKJ61_02510"
     CDS             23417..24208
                     /locus_tag="PKJ61_02510"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HOC12734.1"
                     /translation="MSSLRIALACLPFPESPDASVTAAVAAIAEASAAGAGLVCFPEC
                     FVPGYRALGAPVPVMPAEWLATAHTTIADAARASGVAVVLGTERYVGDALRITALVID
                     PDGEQLGWQDKVQLDPSEDHLYEPAPSGREVFRIGDLVLGVVICHEGFRYPETVREAV
                     AKGAQLVVFPHYSEPLPGHFQPTHYGDPRLSFHESAVMGRAAENTCWVAGVNCAVAEA
                     PTSAIARPDGSLLAWRPHGEPGILVADLDLSEATHRMAKRFRPSA"
     gene            24219..25139
                     /gene="fmt"
                     /locus_tag="PKJ61_02515"
     CDS             24219..25139
                     /gene="fmt"
                     /locus_tag="PKJ61_02515"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOC12735.1"
                     /translation="MRLVFAGTPQVAADTLAHLLDHGTHEVVAVLTRPDAPQGRSARP
                     VPSPVAQLAAVRGIEVLRPARAGDPDLLARLTELAPDCCPVVAYGGLIPQRLLELPSH
                     GWVNVHFSLLPRWRGAAPVQHAILAGDPVTGVTVFKLVPELDAGPVLATADYPLGADE
                     TSGEALSALQAIGAQLLLQTLDGIGAGRIQAVPQPVDDITLAPKLTVPGARVDWNRPA
                     EEIARQVRANNPSPMAWTLLQGERFRVLRATPAEAAGLAPGQVRAGKREVLVGTGAGT
                     LRLEQVQPAGKRPLAAADWARGLRGEAVFE"
     gene            25136..26455
                     /locus_tag="PKJ61_02520"
     CDS             25136..26455
                     /locus_tag="PKJ61_02520"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009639779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HOC12736.1"
                     /translation="MSRPKRRRDQARTAAFRVLRAVTADGAYANLELARQRAELSGAD
                     ATFTTELVSGTCRAMGSYDQVIERASGRPLKSLQPAVVDVLRLGTHQLLAMRVPTHAA
                     ISASVDLAALEVGERVAGVVNAVLRKVAARDWDDWLDPLSEGLDEFGRLALVHQHPRW
                     IVEAWSRVLPAAELADALAADNVAPTPHLVVRPGLAEVSELGGEPARWSPYGAYAGGD
                     PGQLAAVDEGRAGVQDEGSQLVALALSRVDAPDGWWLDLCAGPGGKSALLAGLAREQG
                     GSLVAAELQEHRARLVADNLRAYPDPKPVCLVADGTRPAWRSGGFSKVLADVPCTGLG
                     ALRRRPESRWRRRPEDLPGLIELQGKLLASAIAATAPGGVVAYVTCSPHPDETAAVVA
                     GASGVVTLRAADYLPEVPNCASGDFVQLWPHRHGTDAMFLALLRRST"
     gene            26582..27244
                     /gene="rpe"
                     /locus_tag="PKJ61_02525"
     CDS             26582..27244
                     /gene="rpe"
                     /locus_tag="PKJ61_02525"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOC12737.1"
                     /translation="MGIRITPSILNAELGGLNAEIARVPSADGVHLDVMDNHFVPNLT
                     FGVPMVESIRRNTTKFLDVHLMIAQADRWAPAYAEAGAESVTFHIEASDAPVKLAREL
                     RGHGARASMALRPATPIEPYEDLLGELDMVLLMTVEPGFGGQKFLDLVLPKIRRTRKL
                     LDARGGDIWLQVDGGVSAETIERCVEAGADTFVAGSAVFSSDDPDAVVRALKAQAEGV
                     AS"
     gene            complement(27382..28221)
                     /locus_tag="PKJ61_02530"
     CDS             complement(27382..28221)
                     /locus_tag="PKJ61_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HOC12738.1"
                     /translation="MAEARGLRGLRNPAVIAAFGGWNDAGDAASGVIDHLVDTYRAEL
                     EFAIDPEEFYDFQVNRPTTRLIDGKRTIEWPTTEVLVANLPERDLVLIGGPEPNYRWQ
                     EYLNRLMSLLRSVRPEMVVVLGALLTDAPHTRPVPVSGTASDAELAAELGLEISDYEG
                     PTGIVGVLSQECSVAGMPVVSMWASVPHYVAEPPNPKATLALMTRLEDVLNLPLDPGE
                     LPEAAESWVEQVNELVADDPDIAGYIAALEERRDEATPAPSGDAIAAEFERYLRRRDR
                     RRG"
     gene            28260..31730
                     /gene="metH"
                     /locus_tag="PKJ61_02535"
     CDS             28260..31730
                     /gene="metH"
                     /locus_tag="PKJ61_02535"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015070462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HOC12739.1"
                     /translation="MTRDLRSALTTRVLVADGGMGTMLQGYDLTMDDFQGLEGCNEVL
                     NVTRPDVVGAIHAAYLDAGADLVETNTFGANLSALGEYDATERIAELAEAGARIARAA
                     ADAASTPDRSRYVLGSIGPGTKLPSLGHLPYATLRDAYQVQAEAMLAGGIDGFQIETS
                     QDLLQAKAAVNGARRARAALGVNVPIFVNVTVEASGTMLLGSEIGAALTALEPLEIDA
                     IGLNCGTGPTEMSEHLRHLSRHARIPLVCMPNAGLPELTPDGARYPMGPEEFVGYVAQ
                     YTSEFGLGLVGGCCGTTPAHIGALAAAVAGRPPAPRDPVRVSAVSSLYSEVPLRQELS
                     YLSIGERTNANGSKAFREAMLAENWADCLDIAKQQSRGGAHLLDLCVDYVGRDGAADM
                     AELAFRLGTGVSLPIMLDSTEPAVLQAGLERLGGRCVINSVNFEDGDGPDSRFAKAMP
                     LVKEHGTAVVALTIDEEGQARTAEWKVRVADRLIADLTTKWGLEIGDIIVDCLTFPIA
                     TGQEETRRDALETIEAIRQVTSKYPGIHTTLGVSNVSFGLNPAARQVLNSVFLHECVE
                     AGLDSGIVPAARIVPIARIPEEQRQVAEDLVYDRRTPDYDPLTRFLELFEGVTTTSTK
                     EARAAELAALPVTERLQRRIVDGDAKGLGNDLDDALAAKDALAIINDDLLAGMKVVGE
                     LFGSGQMQLPFVLQSAEVMKMAVAHLEPHLDKADAAGKGTLVIATVKGDVHDIGKNLV
                     DIILTNNGYTVVNLGIKQPVSAIIEAALVHNADAIGMSGLLVKSTVVMKDNLLELNNR
                     GLGGFPVLLGGAALTRAFVEEDLNDLYQGEVRYARDAFEGLALMEKVMAVKSGVPGAA
                     LPEPRRRRVVAKPRANDPEAVDSTERSDVARDQDVPTPPFWGSRVVKGLKLAEVAGWL
                     DERATFMGQWGLRGSSGGPTYEELVETEGRPRLRHWLDRVQTEALAEPAVVYGYWPCH
                     SEGNTLILGDPDDPAREVTRFIWPRQSRDRRLCLADFFRDADEAAERGPDVIALQLVT
                     MGSAVAQATTKLFEADAYRDYLELHGLSVQLTEALAEYWHARVRAELGLGGDGEVDAM
                     IRDQAYRGSRYSFGYPACPDLADRAKLVTLLRPERIGVTLSEELQLHPEQSTDAIVVH
                     HPEAKYFNAR"
     gene            31720..33207
                     /locus_tag="PKJ61_02540"
     CDS             31720..33207
                     /locus_tag="PKJ61_02540"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOC12740.1"
                     /translation="MPADRLRKLATALVLAVALAGCTPTVVEPTQSPAVTPTARPFTL
                     ITSDPVTTADPVIAVGDTDSLLVNSVYQRLMLVLPGSGELKPDLATDCLFTSETVYEC
                     TLPEELTFHNGNVLDSSDVKFSIQRALRMGAANTSIGLLSALKRIETPDAQTVKFYLS
                     WADNQFGYALAGQAASIVDEQVYDPDAALPLDVQPVGSGQFQVFEIAAEQVDFTKFET
                     YFGPRAGILDAVRLQVVPDSVAAEAAIADGTADVVWRSVDGAALQRLTDEIAASPDKT
                     TASGFTSYHLPGARVTQLIWNANSKLRENNDLRLGVAKALQGDRTLDSIVPVGVVDHA
                     ASFALGGRPKLPELKGKRIKLTLGYTATAPGHADQARLLRDRIESMDKVSVRLVTTGD
                     ADLWLTDRPAWVNNATGWLQRYLAEPLAGSSEKLDELVQRSRTTTGAARTVAFTELQQ
                     QAAADNTVLPVSQGDGILLLGKGVTLVGDAFGSGQQLGLWGISHA"
     gene            33236..34117
                     /locus_tag="PKJ61_02545"
                     /pseudo
     CDS             33236..34117
                     /locus_tag="PKJ61_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002534941.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
     gene            34110..35735
                     /gene="arc"
                     /locus_tag="PKJ61_02550"
     CDS             34110..35735
                     /gene="arc"
                     /locus_tag="PKJ61_02550"
                     /EC_number="5.6.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000502 - proteasome complex [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome ATPase"
                     /protein_id="HOC12741.1"
                     /translation="MPEPAELARRLEVTERRLADARSDARTLAATNQKLNATLVTARD
                     ALDSLRAEVAALGQPPNTFGYVVGTGDGTIDVATNGRWLRCALSPDLADQRFVVGDRV
                     LLNEAQLVVGIANEPGVGETARLVSTLPESPLRLLVTTATGEETVVLASTSLNPAELR
                     PGTPLLVDRRAGLALVVLPNNEVADLTLEDVPDVSYADIGGLSDQIEQIRDAVELPFR
                     HRELFAEFGLRPPKGVLLYGPPGCGKTMIAKAVANSAGSHFLNIKGPELLNKYVGETE
                     RQIRAIFTRAKEHATSGRPVIVFFDEMDALFRTRGSGVSSDVEATVVPQLLAEIDGVE
                     DLANVIVIGATNREDLIDPAILRPGRLDVKIKLDRPDRSAAAEILARYLTEATPLNPG
                     EAALRQAQGSLAGTQGSLAEPVEARTDFREQLIDAIVDRLFARTDANRFIEVTYAGGD
                     KETLYVGDFVSGAMLAGVVDRAKKAAIKELINGGERGVRLAHVEAACAAEIGENEELP
                     NTTNPDDWARVSGRKGERIVFLRTLVGARALNP"
     gene            35732..37159
                     /locus_tag="PKJ61_02555"
     CDS             35732..37159
                     /locus_tag="PKJ61_02555"
                     /inference="COORDINATES: protein motif:HMM:NF015119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome accessory factor PafA2 family
                     protein"
                     /protein_id="HOC12742.1"
                     /translation="MTSRSTTSPPPFRSVFGIETEYGIHAPTAPEVHPSVLSAAVVGA
                     YPGVATGSQLDWVEPIPIEDAHNRVLANGARLYVDHAHPEYSGPEVTTAADAVAYDLA
                     GDAAVVAGAKAASAQLGFDIAIYKNNTDSHGASYGCHENHLLPRSVPWTRIASVLPAF
                     LVSRTVLIGAGRVGLGQHGERAGFQLSQRADFFETLEGIQTTKRRPILNTRDEPHAPP
                     SRYRRLHVITADANRNQFATWLKVGSLASLLAALVHDAVPVITLTHPVAAFQAISHDP
                     SLTQAVETSQGRMTALDLQEALLTAVLTHAERAPEMHEASVLAGWTQTLADLRTDPSS
                     CADRLDWVAKYELMRAFADRHDLSWEDLRLAALDLAWARLDAPGPFDALTRAGHQRAV
                     VSPADVAGASASPPADTRAWLRGTLVGRIPDRVLAASWDSMTVLDAHGSRRHLNLAEP
                     LRHTRAEVGEPTDAATVSALLRQLG"
     gene            37193..37369
                     /locus_tag="PKJ61_02560"
     CDS             37193..37369
                     /locus_tag="PKJ61_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019434989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like protein Pup"
                     /protein_id="HOC12743.1"
                     /translation="MTERVHAEQVRARTEDTVEETVKTSRAIDYSAVLDEIDAVLEAD
                     AEEYVRSFVQKGGQ"
     gene            37366..38445
                     /locus_tag="PKJ61_02565"
     CDS             37366..38445
                     /locus_tag="PKJ61_02565"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013161024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HOC12744.1"
                     /translation="MNAPVLSPALVIGTGLVGASVAHALTRAGVTVHLSDAVTSHARV
                     AASRGAGTTTPPVAADVQLVVVAVPPHALAKVIAKSLRTFPNATVTDVGSVKGTVLAA
                     LRATDADLSRYVGSHPMAGSQRVGPLTANADLFIDRTWVVTPHDTSAAHAVLAVQELA
                     TLCGARRVTMGAAHHDEAVAQVSHLPHLMSVLVAGTLTRLPAEHLRLAGQGVRDVTRI
                     AGSDPKLWQQIIGANSAAIRRELVEIQAELADMLAALDAGDLAEVLERGLAGTKALPG
                     KHGLSPTNYAQVVVEIPDAPGALARLFRDIELAGVNVEDVSIDHDLEREVGYLSVDVA
                     PDRAPGLAEAMTAAGWTVRDREPQP"
     gene            38468..39139
                     /gene="cmk"
                     /locus_tag="PKJ61_02570"
     CDS             38468..39139
                     /gene="cmk"
                     /locus_tag="PKJ61_02570"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HOC12745.1"
                     /translation="MTERLVIAIDGPSGSGKSSTAKGVASALGLAYLDTGAMYRAVTC
                     AYLDAGVTPANEAGVIDATTSARLEISTDASDQWVRVNGADVTQRIREPEISANVSAV
                     STVAACRADLVARQRAIIDAAEVGIVAEGRDITTVVAPDAHVRLLLTADPEARMARRK
                     AELGEKVDDTQLADQILRRDSDDSKLVNFTTAADGVTHLDSTFLDLEETIQAVLDLVR
                     RTINA"
     gene            39404..40747
                     /gene="der"
                     /locus_tag="PKJ61_02575"
     CDS             39404..40747
                     /gene="der"
                     /locus_tag="PKJ61_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOC12746.1"
                     /translation="MTELNEPDAVKPVVAVVGRPNVGKSMLVNRIIGRREAVVQDLPG
                     VTRDRVSYDAEWNGREFVIVDTGGWAPDAKGMAAQIAEQAELAIAASDVVLFVVDATV
                     GTQDVDEAVVEVLRRSKKPVVLAANKVDDTRQEADAAGMWNLGLGEPHPVSAMHGRGT
                     GDLLDALLEVLPDAPRMGEPEVGGPRRVAIIGRPNVGKSSLLNQVANSRRAVVDNVAG
                     TTVDPVDELVEVGGVIYRFIDTAGLRRRVKEASGHEYYASLRTQGAIERAEVCVVVLD
                     SSETISDQDLRILTMVEEAGKALVIAYNKWDLTDEERRHYLSREIEQDIRAFAWAPSV
                     NISALTGRNVDKLSKAIEEALEGWETRVSTGKLNAFLGRLAAAHPHPVRGGKQPRILF
                     GTQAHNCPPTFALFTSGQFDPQYVRFVERRLREDFGFRGSPVHVEVRAREKRKNR"
     gene            complement(40931..41305)
                     /locus_tag="PKJ61_02580"
                     /pseudo
     CDS             complement(40931..41305)
                     /locus_tag="PKJ61_02580"
                     /inference="COORDINATES: protein motif:HMM:NF024773.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
     gene            complement(41321..>41767)
                     /locus_tag="PKJ61_02585"
     CDS             complement(41321..>41767)
                     /locus_tag="PKJ61_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC12747.1"
                     /translation="KKVRVAPVSGKITAVTWFRGQGGRKFQLSVNDGYLRVLRPEVNL
                     RLDLKASGLADKTVHPSVQAVMSANGRLWVLIVGFVELTPDTNQTVAGLISVSASGAV
                     SAVQPVRDPTSPADPGDGHNLGIRYGGTHPTMMFIDPGAVRVHRKR"
BASE COUNT         6647 a        14136 c        14622 g         6362 t
ORIGIN      
        1 caccgagaac ccgtccttct cggtgatctc gccgctgctg agccgttcgc tgctgctgac
       61 cctgcagccg ctgaccgacg ccgacatcgg cgtgctgctc gatcgagctc tcaccgaaga
      121 gcgtggcctc ggcggggcgt tcacgctcac cgcggacgcg cggacggccc tgctccgttt
      181 cgccggcggc gatgcccgtc gggccctgac ctacctggag gaagccgccg ccggcgccga
      241 agctcaggac accagcgaga tcgacgcgga ggtggtcggg gcagccgtcg acaaagcggc
      301 cgtccgctac gaccgggacg gtgaccagca ctacgacgtg atcagcgctt tcattaagag
      361 tctgcgcggc tcggacgtcc aggccgggct gcactacctg gcgcgaatgc tggaggcggg
      421 ggaggacccg cggttcgtcg cgcggcggtt gatcattctc gccagcgagg acatcggcat
      481 ggctgcgcct tcggtactgc agacctgcgt ggccgcagcg caggcagtcg cgctgatcgg
      541 tatgccggaa gctcggatca acctcgcgca ggcggtggtc gcctgtgcgc tggcgcccaa
      601 gtcgaacgcg gtgatcaaag ggatcgacgc tgccatcgcc gacgtccgcg ccggccggat
      661 cgggacggtg ccgccgcacc tgcgcgacgc ccactacccg ggtgccaagg ctcacggcca
      721 cgggtcgggc taccagtacg cccacgacgc gccgcacggc gtggccgccc agcagtacct
      781 gccagacgaa ctggccggcg cgaagtacta cgagccgacc gaccacggca acgaagcggc
      841 gctcgccgag cggctggcga tgctcgaaga gcttttgggt aaagacaatt cctgaccagc
      901 cgataggctg cgcaggtaca cccggatccc agtttgtgag gtagccaatg tccgccggag
      961 aggtcgcagg tctgattgca gcgatggctg cggtcgcgtt tgtcgtgttc tgcgcggtcc
     1021 cactgctcaa gctgggtcgg gtgctcgagg aactgcgctt ggcggtgcgg gatctcggcc
     1081 acaactcggt gccgattctg caggagctgc ggggaacggt cgccgcgacc aacagtgaac
     1141 tcggcaagct cagcctggtc accgaggatg tcgcgaaggt cagcgcccac gccaccgtgg
     1201 tcagcgagaa cgcggcccag ctgtcgacgc tgttcacttc gaccctcggc ggtccgatga
     1261 tcaagacggc ggccttcacc tacggcgttc gccaggcggt gggcggtcgc aagccgagca
     1321 agaaggccgg caagtgatgg gcaaacgact cttctggttc gcggttggtg ttggcctgac
     1381 cgcactggta gtggtgaagg gccgcgagta ctacgagcgc ttcacgccca agggggtggc
     1441 cgagcagcta gagaagaccc gcagcagcgc gtccgcctgg ctgggtgact tcttcgagac
     1501 catgaacgaa gccatggacg agcgcgaggg tgagttgcgg gaggctctcg gcctggtcga
     1561 gtgaatccca cccgaccagg tgaccaccag ccgcgaacac caccgccggc gagcaacgcc
     1621 ggttgaacct cgaaagacac gtgaaccatg aaaactgctg acatccgcca gcgcttcctc
     1681 gacttcttcg cggcgcgcga gcacaccatc gtgccgtcga ccccgctggt ctacaacgac
     1741 ccgaccctgc tcttcgtcaa cgccggcatg gtgccgttca agccgtactt caccggcgcg
     1801 gaggacgcgc ccttcaaacg tgcggtgagc gtgcagaagt gcgttcgcac cctcgatatc
     1861 gaagaggtcg gcaagaccac ccggcacggc accttcttcc agatgaacgg caacttctcc
     1921 ttcggcgact acttcaagga aggcgccatc gagtacgcct gggagttggt caccgggtcg
     1981 ccggccgagg gtaactacgg cttcgacccc gacaaggtgt gggtcaccgt ctacctcgac
     2041 gacgacgagg cggccgggct gtggcgcaag gtgggcatcc cggatgagcg catccagcgg
     2101 cgcgggctga aggacaacta ctggcacatg ggcattcccg gcccgggcgg cccgtgcagc
     2161 gagatctaca tcgaccgcgg ccccgagttc ggcccggacg gtggcccgat cgtggacgag
     2221 gaccgcttcc tggagatctg gaacctggtc ttccagaccg aggagctttc cgccgtccgc
     2281 gccaaggacg acttcgacgt cgcccgtccg ctgccggcca agaacatcga caccggcatg
     2341 ggcctggagc gcaccgcgta cctgctgcag ggcgtggcca acatgtacga gatcgacgag
     2401 atctttccgg tgatcaagca cgccgccgaa ctcgccggca ccacctacgg caaggatcag
     2461 caagctgaca tccggctgcg ggtggtcggc gaccacgtcc gctcggcgct gatgctgatg
     2521 accgacgggg tgaccccggg caacgaggcg cgcggctacg tgctgcgccg gctgctgcgc
     2581 cgcagcatcc gctcgatgcg tctgctcggc gtccaggaca aggtgctgcc cgagctgctg
     2641 ccgaccagcc gcgacctgat ggccacgtcc tacccggaga tcaccgagca ttgggcccgg
     2701 gtcagccagg tcgcctacgc cgaggaggag gcgttcgccc ggacgctggc cgccggcacc
     2761 cagatcttcg acctcgccgc cggccaggcc aagaccgagc aggtcaccga gatcagcggt
     2821 gaccgggcct tcgccctgca cgacacctac ggcttcccga tcgacctgac cctggagatg
     2881 gccgccgagc agggcctcag cgtcgaccag gcgggtttcc gccggttgat gcaggagcag
     2941 aaggaccggg ccaaggccga cgcgcgcgcc aagaagggcg cgacggtagc caccgaggcc
     3001 taccggacgc tgcgcagcgc cggcgaggtg cctttcctcg gcttcaccga cctgaccgcg
     3061 gacgcgaccg tccgcggcat cgtcgccggt ggccaactgg tcaaccgtgc ggcgaccggt
     3121 gacgtggtcg aggtggtgct cgaccagacc ccgttctacg ccgaagctgg cggccaggac
     3181 gccgacaccg gcctgatcac cggtgacggc gccaccctgg aggtgctgga cgtgcagcgt
     3241 cccgtccagg gcctggtggt gcacaaggtg cgggtcgccg acggcgaact cgcctccggc
     3301 gcccggatgg tggcgcaggt ggacgcggcc gcgcgggccg gtgcctgcca ggcgcacacc
     3361 gccacccaca tcgtcaacgc caccctgcgg cagttgctgg gggagtccac ccaccaggcg
     3421 ggctcgtaca acaagcccgg ctacctgcgc ttcgacttca acgccaccgg cgcactcagc
     3481 cacggcctcc gcgaggaact cgaaggcgtg gccaacgagg cgatccgcgc cgactacgag
     3541 gtgaccgcgc aggagatgcc gctggcccag gccagggcgc tcggtgcgca ggcgatgttc
     3601 ggtgagaagt acggcgacat cgtccggatg gtcgaactgg ccgggccgtg gtcgcgcgaa
     3661 ctgtgtggcg gcacccacgt gaagcgcacc tcgcagatcg ggctgctcag cctgctgggc
     3721 gaggcttcgg tgggctctgg tctgcgtcgg gtggaggctt tcgtctcggc ggacgcgttc
     3781 gcgcaccttg cgaaggagcg cgcgctcgtc gccgggctgg ccgacctgct gaaggtgcag
     3841 cccgaccagc tcagcgaccg cgtcgagaag ctggtcgccc aggtcaaggc cgccgagaag
     3901 gaaatcgccg cgctgcgcag ccgcgaactg ctggccaacg tgccggggct ggtcgcggcc
     3961 gcgacttcga ctggtgcgta cgagctggtc agcaagcacc tgcccggtac ttcggcggac
     4021 gacctgcgct ccctggccat ggaagtgcgg acggccttcg gcgcgcgtcc gggtgtggtt
     4081 gcgctgatcg gcggcgatgc gaagccggcg ctgatcgtgg ccacgacggc aacggcccgg
     4141 gagttgggcg cgaaggccgg cgccctggtg ggcgtgggtg cggcagcgct cggtggccgt
     4201 ggcggtggcc gggacgacct tgcccagggc ggcggcgcca acgccgacgc ggcaccggcg
     4261 gcactggctg cgatcagcgc cgcgctggcg ggctgagcgt gcgcaccggc gtccggctgg
     4321 cgctcgactg gggcaaggcc cggatcgggg tcgcggcgag cgacccgtcc ggcatcctgg
     4381 cgcacccggt ggagacgatc ccggccggcc cggacgcctt cgcgcgggta cagcggctgg
     4441 tagccgagta cgagccgatc gaggtggtgc tcgggctgcc gcgcaacctg gccggcgttg
     4501 agggcccggc cgcgctggcg atccgccagg tcgcggccga gttggtggca aagttgggcg
     4561 cgctacccgt ccggctggtg gacgagcgac tgaccaccgt gaccgcgtcc cggcaactca
     4621 gcggcgccgg acgcaacacc cgcaagcagc gcgccgtgat tgaccaggcc gcggccgtgg
     4681 cactcttgga acaggctctg cagtacgagc gcaccaccgg actaccgctc ggcagcatcg
     4741 tcgagccccg cgaggaaacc aggaaggatt ccagcgcatg agcaaccgaa tgcccggaga
     4801 gattcgagac ccggacaccg gaaagatcga ctacaaggag atctggtaca agctgcgctc
     4861 cgccttcgcg gtgctgctgt cgctggctgt gctcgctggc gcgggctggt tcgtctacga
     4921 caaggcccgg ggcgcctgga tggagttccg caccgcggag gactacatcg gcgaaggggt
     4981 ggccccggtc gaggtgacga ttcccgaggg cgcgacgctc ggtcagatct cggacatcct
     5041 cgtgcaggcg aaggtggtca agaccgccaa ggcgttcgat cgcgaggccg aggccaacgc
     5101 agattcgaag acgatccagc ccggcaaacg catgatgaag accgagctgc cggccaaggt
     5161 cgccttgagc atgttgctgg acaagaacct catcatccgc aaccgcttca cccttgccga
     5221 aggcaagtgg ctcgctcagc agtacccggt gatggagaag gccaccggcg tcacggtgaa
     5281 ggagttcaag gccgccgcca aggactggaa gaagctcggc ctgccaacct gggccgagca
     5341 cggcctggaa ggcttcctgt tcccggacac ctacgaggtc ccggagaagc ccaccgccaa
     5401 gtcggtgatc aagatggcca ccaagcagtt cgccgcggtg gccaaggaac tggatctgga
     5461 ggccacggcc gaggagatgg ggcgcacgcc ctatcaggtg ctggtgatgg cgagcatcgt
     5521 cgagaaggag accttcaagg ccgaggaccg cccgaaggtt gcccgggtga tcctcaatcg
     5581 gctgaaggcg gggaccccgt tgggcatgga ctcggtcatc gcctacgcag tgaagaagtc
     5641 cggcgtcctc gaactgaccg ctgaggacct ggccatcgac tcgccctaca acacccgcac
     5701 ccgcaagggg ttgccgccca cgccgatcaa cagcccgacg aagtccgcca tcgaggccgc
     5761 gttgcacccg gctgagggtg actggacgta cttcatcacg gtcgatccct cgagcggcga
     5821 aacgttgttc accgccaact acgacgagtt cctggcgggg aagcagaagt acaaggactg
     5881 gtgcgaagca agcaaagaga accgcaaggt ttgttacgga gagtaggcga atgcttcgtt
     5941 acctgactgc aggggaatcc cacggccgag ccctggttgc gaccatcgag gggctgcccg
     6001 cgcacgtgcg gatcaccacc gctgacatcg cggacgcgct cgcgcgacgg cggctgggca
     6061 ccggtcgcgg cgctcggatg aagttcgagg ccgacgaggt cacgctgctc accggagtgc
     6121 gtcacggcga gaccctgggt tcgccggtgg cgatcatggt gggcaactcg gagtggccca
     6181 ggtgggagac cgtgatggct cccgacccgg tggacgcagc cgaactcgag cagcttgccc
     6241 gcaacgcagc gctcacccgc ccgcgtcccg ggcacgccga cctcgcgggc atgcagaagt
     6301 acgactggga cgaatcccgg ccgatccttg aacgcgcgtc cgcacgggag accgcggcca
     6361 gagtcgcact cggacggttg gcggcgaact tccttgagca ggcgttcggc attcaggtac
     6421 tcagccatgt ggtgcggatt ggtggcgcgg cggtcggcga ctcaggccct tcgtcccttc
     6481 gacacgctca gggtgaacca agctcaggga gcgttccgct gccgggtgat ctggctgcca
     6541 tcgacgcaca ccccgtccgc tgtttcgact cggttgccgg tgcggccatg ctcgccgaga
     6601 tcgccacggc gaaggccgag ggggacaccc tcggtggggt cgtggaggtg ctggcctacg
     6661 gcgtacccgc cggactgggt tcgcacatcc agtgggaccg gcgtttggac gcgaagctcg
     6721 cgcaggcatt gatgggcatc caggcgatca agggcgttga ggtcggggac ggcttcgggc
     6781 tggcggcaac gcgcggcagc ctcgcccacg acgagatggt ccccggcgcg gatggcgtcg
     6841 agcggctctc gaatcgctcc ggcggcaccg agggtggcat gagcaacggc gagccgctgc
     6901 gcgtccgggc tgccatgaag ccgatcgcga ccgtcccgcg ggccctgcgc acgatcgacg
     6961 tgagcaccgg tgaggccgcc caggcgcacc accagcgctc cgacatctgc gcggtaccgg
     7021 ccgccggggt ggtcgccgag gcgatggtcg cgctggtgct cgccgaggtt gcgctggaga
     7081 agttcggcgg ggactcggtg gccgagaccc ggcgcaacta cgaggcctac ctgagccggt
     7141 tggccgagcg gcgactggct tgacccctgc gctcgcgctg atcggcatcc ccgggtcggg
     7201 taagacaacg gtgggcgaac tgctcgccgc gcaactccaa ctgccgttca tcgacgtggc
     7261 cgaactggtg gaagccgaag tgggcggctc cgcgccggag ttcttcgccg gtgccggcga
     7321 ggctgcctac cgtgagatcg aggaagccgt gacgctcgcg gccctggatg agactgcagt
     7381 gattgcgctg agcagcggtg cgatcaattc ggcagccgtg cgaggagtgc tgggcggcga
     7441 agtcgtctgg ctgcgcacca acgtctcgac ggccacccgg cggctgagca tgaatgcgct
     7501 gggcatggcg gcgctggtcg ccattcggaa tcgcatggac gcgatgctga gccaacgggc
     7561 accctggtac tccgaagtgg ccacccaggt ggtggacact gaccggttga cgccgaccga
     7621 ggtggcggca gccgtcctcg agaaccaggg agggcactga tgacttggga gccgatcgac
     7681 gtcgcgaccg aacagcccta ccaggtactg atcgactccg gtgtggcgaa gctcctgccc
     7741 cagttcctgg acggcgtgga tcgggtcgct gtcatccacc cgccctctct ggccgacaag
     7801 ctcgcggtcg tgaccgcagg gctggatcag cagatcacca cgatcccggt gccggacggc
     7861 gagagcggca agaccgccca cgtgctgacc ggctgctggg aggcgctggc cgaggccggc
     7921 ttcacccggt cggacgcgat cgttggcctg ggcggtggtg cggctaccga cctggccggc
     7981 ttcgtcgcgg ccacctggct gcgcggcgtg aagtacatcg cactaccgac caccctgttg
     8041 gccatggtgg acgcggccgt cggcggtaag accggcatca acctggccgc aggcaagaac
     8101 ctcgtgggcg ccttccacga gccgttcact gtgctcggcg acctggattt cctgcatacc
     8161 ctgcccgccg gcgagctgcg ttcaggcatg gccgaggtgc tgaagtgcgg cttcatcgcc
     8221 gatccggaga tcctcaccga gttcgaagcc gatgtcacgg cggcgctgca accagccagc
     8281 gcggtgcagg caaagctgat ccgccgcgcg gtccgggtca aggcggcggt ggtcgccagc
     8341 gacctgcgcg agcggacgtc tgagggcggc aacatcgggc gagaggcact caactacggg
     8401 cacacgctcg gacacgccat cgaacgctcg gagcacttcc gctggcgtca cggtgaagcg
     8461 gttgcggtag gcatggtctt cgccgcgacg ctggcccatt cggcggggct gatcgacgcc
     8521 gaactgctgg cccgccatcg gcatgtgctg gaactcgccg ggctgcctac ccgctacccg
     8581 caagggccgt ggttggagtt gcgcgacgcg atgaacctgg acaagaagtc ccgcggatcc
     8641 agccttcggt tcgtggtgct gggtggcttg ggcatgcccc gcattctgca gggttcgagc
     8701 gagggcgagt tggctgacgc ctacctcacc atgaactcga tctgagcggg cgccgatgta
     8761 cgtgcctgtg cacttcgcga tgagtgccga ccagatcgcg gacgtgctcg cgtccgcgcc
     8821 tgccggggac ctggtgaccg acggtccgga cggtctgacc gcaacctacg tgccgatgct
     8881 ctgggacccg gaatccggca gcctgatcgg ccacctgtcc agggtgaacg cgcagtggcg
     8941 cgacgccggc gaagccctgt tcattgtcca cgggccggac gactacatcg cggccgactg
     9001 gctcagcaca ccggcagcgg acagcgtccc gacctggaac tacgtcaccg tccacgttta
     9061 cggcacgctg gtcgcccacg acgatccgga ctggacgctg gccgccgtcc gccggctgag
     9121 cagagcgcac agcgacccgg ccatcgacga tctgcctgcc gacgaggtgg ccaagctgtt
     9181 gcgggccgtg gtgggcgtgg agttgcgtga cctgcggatg gtcggcaagg ccaagatgag
     9241 ccagaacaag tcgccgcagg tggtcggcca ggtgatcgac ggactgcggg acgccggtgg
     9301 ggtcgacacc gctgcctgga tggcggagca ctcgctaccg cgagccgagg cgaaggctgc
     9361 gctgttggcc gccatccgcg agcgcggcta gactgagcga ccgcgattgc tcagctaacg
     9421 aaagcggact cctcgaatgg caaccatttc caccaatgac ctcaagaacg gcgtggtcct
     9481 cgacatcgac ggccagcttt ggaccgtgat ctggttccag caccacaagc cgggcaaggg
     9541 caacaccgtc gtccgcagca agctgaagca cgtgctcacc ggcaaggtcg tggatcgcac
     9601 cttcaactcc gacaccaagg tggagtcggc gaacgtcgac cgccgcgaga tgcagtacct
     9661 gtaccacgac ggcgaggcgt acgtcttcat ggacaccgac acctacgacc agctcaacct
     9721 gagcgaggaa gtggtcgggg acgcgaagaa ctacctgctg gaaaaccagg tggccaccgt
     9781 cgcgctgcac gaaggcaacc cgctgtacat cgacctgccc acctcagtgg aactcgagat
     9841 cacctacacc gagccgggcc tgcagggtga ccgctccacg ggcggcacca agcccgcgac
     9901 cgtggagacc ggcctgcaga tccaggtgcc gctctttctc accaccggtg agcgggtcaa
     9961 ggtggacacc cgtgacggca gctaccttgg tcgggtctga gtgacctctc ccgagccggg
    10021 caagaagctc tccacccgca cgaaggctcg caagcgggcc ctcgacatcc tgtttgaagc
    10081 cgacttgcgc gggctgcccg cgaccgaggt gctcgccgcg cacaccgctg aagccgatcc
    10141 gccggtgcgg ccgttcacgg ccgacctggt gcgcggtacc ttcacgaatc tgcggctgct
    10201 ggacacgctg atcgccagcc acctgccgtc cggctggacg ctggaacgga tgccccgggt
    10261 cgatcgcaac cttgcccgcc tgaccgccta cgaactcggt cacaccgaga tcgacaccgc
    10321 gatcgcgctg gccgagtgca tgaccctggc cggcgagctc tccaccgacg actccccggg
    10381 cttcctgaat ggcgtcctgg ccgcggtggc ccgcgacgtc cgtccggcct aaccactcgg
    10441 tccaccggcc acaaagcgaa gtcgtttgaa cttgccagta gaactgcgcc ttggggtaac
    10501 cacggactgg cgcgcgatcg gagcgtccgg aacggtttgc tagtctcggc gctggcactc
    10561 gcccgcttat ctcgtaaagg acgtccatgc ccagcagcct gttcagcacc tcggcgttac
    10621 cggctttgct ggttctggag gacggcaggt ggttccgcgg cagttcgttc ggcgctgcgg
    10681 gggagagctt cggcgaggcg gtcttcaaca ccggaatgtc gggctaccag gagaccctca
    10741 ccgatcccag ctaccaccgc caggtcgtcg tggccaccgc gccgcacatc ggcaacaccg
    10801 gctggaacga cgaggacgac gagtccaagc gcatctgggt ggcgggctac gtcgtgcgcg
    10861 acctgtcgcg cgtccgctcg aactggcgct cgaaccgctc cctggacacc gaactgcgca
    10921 accagggcgt ggtgggcatt gccgaggtcg acaccagggc gatcacccgg cacctgcgtg
    10981 agcgcggggc gatgcgggtg gggatctcca ccgtcgaact cgatccggag aaactgctgg
    11041 ccaaggtgct gcaggcgccg ccgatggccg gcgccaacct ggtggccgac gtcacggtga
    11101 ccgaggcttc ggttacgccg gcggtggggg agaagcggtt caccgtcgcg gcgatcgacc
    11161 tcggcatcaa ggacatgacc ccgcagcgga tgagtgagcg tggcatcgag gtgcacgtgc
    11221 tgccagccac ggtgagcgtc gccgagatcc gcgagctcgc cccggacggg gtcttcttct
    11281 ccaacgggcc cggcgaccca gccgcggcga ccgcagcggt cgaactgctc accgaagtgc
    11341 tggacgccgg cctgccctac ttcggcatct gcttcggcaa ccagatcttc ggccgcgcac
    11401 ttggtttcgg cacctacaag ctgaagtacg gccaccgtgg gatcaaccag ccggtgatgg
    11461 acctgaccac ccgaaaggtc gagatcaccg cccacaacca cggcttcgca gtggacgcac
    11521 ccctggaccg gcataccgtc acccgctggg ggaaggcgcg ggtatctcac atcggcctca
    11581 acgaccaggt ggtggagggc ctcgaactgc gtgacgaccg cggcgggctg ctcggctttt
    11641 cggtgcagta ccaccccgag gctgcggccg gcccgcacga tgcggggtac ctcttcgacc
    11701 gcttcgtcga catgatggcg gcccggggcg gccaagaggt ggctgagccc accgaaggcc
    11761 cgccgaccct tcgtcaagct cagggaacgg aagaggacgc ctgatgccac gccgtaccga
    11821 catcaactcg atcctggtga tcgggtccgg cccgatcgtg atcggccagg cctgcgagtt
    11881 cgactactcc ggcacccagg cctgccgcgt gctgcgcgag gagggtttcc gggtgatcct
    11941 ggtgaactcc aacccggcca cgatcatgac cgaccccgag ttcgcggacg ccacctacat
    12001 cgagccgatc cgtcccgaat acctggagca ggtgatcgcc accgagcgtc cggacgcgct
    12061 actggccacc ctgggcggac agaccgcgct gaacgctgcg gtcaccctcg ctgagaccgg
    12121 agtgctggag aagtacggcg tcgagctgat cggagcgtcc atcgaggcga tccagaaggg
    12181 cgaggaccgg gagaagttca aggaaatcgt cgcctccctg gacgtgcccg gcgtcaaccc
    12241 ggaggtcgcc cgctccgcca tctgccacac catggacgag gtgctcgccg ccgctgatca
    12301 gctcggctac ccggtggtcg tccgaccctc cttcacgatg ggcggggtcg gttcgggctt
    12361 cgcgcacgac gagaccgaac tgcgccggat cgcgggccgc ggcctgcagg cgtccccggt
    12421 caccgaggtg ctgatcgagg agtcggtgct cggctggaag gagtacgagc tcgaggtgat
    12481 gcgcgacacc gccgacaacg tggtggtggt ctgcagcatc gagaacctcg acccgatggg
    12541 cgtgcacacc ggcgactcga tcaccgtggc gccggcgatg accctcaccg accgcgagta
    12601 tcaggccatg cgcgacgtcg gcatcgccgt gatccgtgcg gtcggcgtcg acaccggcgg
    12661 ctgcaacatc cagttcgcga tccatcccga caacggccga ctgatcgtga tcgagatgaa
    12721 cccccgggtc tcacgctcgt ccgcgctggc gtccaaggcc accggcttcc cgatcgccaa
    12781 gatcgcggcc aaggtggcgg tcggctacac cctggacgag atccccaacg acatcaccgc
    12841 cgaaacgccg gcctccttcg agccggcgct cgactacgtc gtggtcaagg cgccgcggtt
    12901 cgccttcgag aagttcccga ccgccgattc caccctgacc acgcacatga agagcgtggg
    12961 cgaggctatg gccatcggcc gcaacttcac cgaggcgctc ggcaaggcgc tgcgttcgct
    13021 tgaggacgcg aaggccggtt tcgacttcac cggcgagatc gccgaaagcc tggacgaact
    13081 gctggccacc atgaaccgac cgcacgacgg ccggctgaac gtggtgatgc aggccttccg
    13141 gctgggcgcc accgccgcgc agatccacga cgccaccaag atcgatccct ggttcgtgga
    13201 ccagttgcag ctgatctacg aggtcgccca gcacgtccgc actgcccccg agctgaccgc
    13261 ggacgtgctt cggctggcca agcgccatgg gctgtccgac gggcagatcg ccaagctgcg
    13321 caacctgtcg accgacgtgg tgcggggcgt ccggtgggcg ctcggggtgc gtccggtcta
    13381 caagaccgtg gacacctgcg cggccgagtt cgccgcgcac acgccgtacc actactcctc
    13441 gtacgacgag gagaccgagg tcgagccgcg caccaagccg gccgtgctga tccttggcag
    13501 cgggccgaac cgcatcggcc agggcatcga gttcgactac tcctgcgtgc atgctgcgat
    13561 ggctctgcgg gccgccggct acgaggccgt gatggtcaac tgcaacccgg aaacggtctc
    13621 gaccgactac gacacctccg accggctcta cttcgaaccg ctcacgctgg aggacgtcct
    13681 tgaggtctac cacgccgagg cgcaggccgg cccggtggcc ggagtgatcg tgcagctcgg
    13741 cggtcagacc ccgctaaagc tggcccaggc gctcaaggac gccggcgtgc cgatcgtggg
    13801 gacgccgccg gaggcgatca acctcgccga ggagcggggc gcgttcggca gggtgttggc
    13861 ggacgcgggc ctgccggctc cggcctacgg catggccgag tcggcggagc aggcgctccg
    13921 gatcgcccat gacatcggct acccagtgct ggtgcgcccc tcctatgtgc tcggcggtcg
    13981 cggcatggag atcgtctacg acgacgcctc gctggcctcc tacatcgagc gggcgaccga
    14041 catcaccccc gcccacccgg tgctggtcga ccgcttcctc gacgacgcga tcgagatcga
    14101 cgtggacgcc ctgtacgacg gcaccgacct gtatctcggc ggggtgatgg agcacatcga
    14161 ggaggccggc atccattccg gcgactcggc ctgttcgttg ccgccgatca cgctcggcaa
    14221 cgaggtgatc gagcagatcc gccgctccac cgaggcgatc gccgctggcg tgggcgtgcg
    14281 cggactgctg aacatccagt acgccctgct cggcgacatc ctgtacgtgc ttgaggccaa
    14341 cccgcgggct tcgcggacgg tcccgttcgt ttcgaaggcc accgacaccc agttggccaa
    14401 ggccgccacc cggatctcgc ttggcgcgac gatcgcggaa ctgcgggccg agggcatgct
    14461 gcgtaccggc atggacggca ccgccaccat cgaggctgcg ccgatcgcgg tgaaggaagc
    14521 cgtgatgccg ttcaaccggt tccgtaccgc ggacggctcc tgggtcgaca ccctgctcgg
    14581 ccccgagatg aagtcgaccg gtgaggtgat gggcctcgac ctgaccttcg ggacggcgtt
    14641 cgcgaagagc caggcggcct cgttcggcgc ggtgcccacg tccgggacgg tgttcgtctc
    14701 agtggccaac cgcgacaagc ggcacgcgat cttcccggtg aagcggctgg ccgacctggg
    14761 cttcaaggtg ctggcgaccg aaggcaccgc gcaggtgctg cgccgtcagg acgtggacgt
    14821 caccgtgatc cggaagaact cccaggggcc agggccgaat ggtgagaaga ccatcgtgca
    14881 ggccatcctt gacggcgagg tcgacctgat cttcaacacc ccgcagggcg tctcgaagga
    14941 cggcaacccg cgccgggacg gctgggagat ccgttcggcc gcgatcctcg ccgacgtgcc
    15001 ctgcgtgacc acggtgcagg gtctttccgc ttgtgtgcag ggcatcgagg cactcggccg
    15061 cggcgcggtc ggcgtccgtt cgctgcagga gtggagcgcc ggcgtccgcg cggagctggc
    15121 cggccaggac gccggctgat gggcctaccc gccgggttgc tcaacgccgg ttacgtcgaa
    15181 ctgctgcggc cgctgctgtt cggcagcttc ggcggtgacc cggaggcgat ccacgagcag
    15241 atgatccacg cgctcgcggg tgtgggttcg gccgcgcccg tgcgcgcgct cacctcgctg
    15301 ctggcagcca gccaggggca cccggtccgc gtggccggaa tcaacttccc ggggcgggtc
    15361 ggcctggccg cgggcatgga caaggacgcg tccgcggtgc tggcgtggca gggcctcggt
    15421 ttcggtcacg ccgaactggg caccgtgacc gcgcaggccc agccgggcaa cgaccgtccg
    15481 cgggtgttcc ggctgcgcag caccggcgcg ctgatcaacc gcatgggatt caacaacccg
    15541 ggcgcggcgg cggtggcggc cacgctcgcg tcccgcaacg tctaccgcgg caacggtgtg
    15601 gccgggattc cgatcggcat ctccatcggc aagaccaagg tcgtcgatct ggccgatgcg
    15661 gtcgaggact atctgacctc gctgcgcctt ctggccgcgc acgccgacta tgtcgcggtg
    15721 aacgtctcca gcccgaacac ccctggactg cgtagcctgc aggacgccgg cgccctggcc
    15781 gaactgctcg gtgctctggt cgccgaaacc tgtctcgctt cgcccgcggc ggcggggctg
    15841 ccgatcttcg tcaaggtggc gcccgatctc acctggtgcc agctggacga agtcctcgcc
    15901 actgcgcagg acgcgggcgt ggccggcctg atcgcgacaa acacgacgct ggcaagggac
    15961 ggtctggcgt cggccgacca gcacctggcc gaccaggccg gggggctgtc cggtagcccg
    16021 ctcaccgtcc gcgcgcgcga ggtcgtggcc tacctgaccc ggcacaccga actgccggtg
    16081 atcggggtcg gcggcatcat gacaccgggc gacgcgatgg ccatgttcga cgccggggcc
    16141 caactcgtcc agctttatac tggcttcatc taccgcgggc cgggtctggc cgcagcgatc
    16201 aacgccctcg acgtcgaaag gcaccgatag tgagcaccta ctggcgcagg cttcgtgaag
    16261 tggtcgccga gcgcggcgcg ctctgtgtcg gggtggatcc gcacgtttcg ctgctgaagg
    16321 cgtgggacct ggaggtaagc gctgccggcg cggaacgctg cgcccgcacg gtcgtggagg
    16381 ccctcggcga tctggtcgcg gtgttcaagc cgcaatcggc cttcttcgag gcctatggct
    16441 cggccggcat cgccgccctt gagcgggtgc tggccgacat cagggccacc ggggcgctaa
    16501 gcatcctgga cgtcaagcgc ggcgacatcg gctccaccat ggaggcctac gccgacgcct
    16561 acctcaccga gggctcgccg ctggccgcgg acgcgatcac ggtctcgccc tacctgggct
    16621 tcggctcgct tgcgccggcc atcgagcggg cgcatcgcta cgagcgcggc gtgtacgtac
    16681 tggcccgcac ctccaacccg gagggggcgc aggtgcagct tgcgctcacc caggacgcgt
    16741 ccgtcgtgca gtcgatcatc gacgccgcca ccgaggtcaa ccacgcgtcc aggcagaagg
    16801 cgatcggcct ggtggtgggc ggcacccatg actcgctggg ctgcgatctg gaggaattca
    16861 acgggtcgat cctggttccg ggcatcggcg tacagggtgg acggctggag gacctccccg
    16921 acctcttcgg tgaagcggtg gaccaggtgc tgccgagcgt gagtcgcggc gtgatctcgg
    16981 cagggccgga cgcgtccgcc atgcgtgcca aggtcagcgc gctgctcagc caagcggtgc
    17041 gctgaggtcg ccggaagtcc ttgtggaaac ctttccgttc gcggtgcgca cgggcgtcgg
    17101 taccgatagg ttgtggacac aacgtagacc gaccacctgt tactcgaagg caatcctgtg
    17161 acaattcctc agctcactac agagcaactc gaggccgccc gcgccgcggc cacggaagca
    17221 cggcgtcgcc gcgcagaact caagaccaag gtgcgttctg gcattctgac gctgagccag
    17281 gcgctggaga ccgctgccga ggacgacgtt ctggcgcacg tgaaggtcgt cgacctgctg
    17341 aaggcgctgc cgcgggtggg ggagaagcgc gccgcggtcg tgatggagcg cctcgacatc
    17401 gccccgaacc gacgcatccg ggggctgggc cggcaccagg tggccggcct gaaggccgag
    17461 ttcagtgaca ggtgacgtaa ccgtcatctc cggcccgacg gcggtcggca agggaaccgt
    17521 cgtgaaggcg ttgcgtgccc ggcacccgga agtctgggtc tcggtttccg caaccacccg
    17581 tccaccccgt ccgggtgagg tggacgggga gcactacctg ttcgtgagcg cgaggggatt
    17641 cgacgacctg gtcgcgtccg acgggctact ggaatgggcg ctggtgcacg gcgtcgaccg
    17701 ctacggcacc ccggccgcgc cggtgcaggc cgcggtggcc gccgatcgcc gggtgatcct
    17761 cgagatcgac ctgcagggcg cccgccaggt acgggagcac ctccccgaag ccaggctggt
    17821 gttcatcgcc ccgccgagcc aggagacgct ggtgaaacgg cttgagggcc gtggcaccga
    17881 gaccccggag cagatggccc gccggctgcg gacggccgaa ggcgaacttg ctgcggcggc
    17941 cgagttcgac cacgtgatcg tgaacgacga tttgggctct gcggtggatg agttggtaga
    18001 gttgctaggt ctgtgaagga agcgtccatc gcgaccgcat ctcacccaat caggaagcca
    18061 aacttgagca ctcacattcc cgaggggatc accaacccgc cgatcgacga attgctgacc
    18121 caggtggatt cgaagtaccg actggtgctg ttcgcagcca agcgggctcg gcagatcaac
    18181 gcctactact cgcagctggg cgagggcctg ctcgagaacg tcggcccgct ggtcgacatc
    18241 ggcatccagg agaagccgct ctcgatcgcg ctgcgcgagg tgaacgccgg tgtgttggag
    18301 tgcaccgata tcgatcccga ggctgaggcc gccgcccgcg ctgaggcgtc cgccgatccg
    18361 gacttcgggc tcgacccgtt cggcaccgac gacctcgcct gatccaggca gatgagccgg
    18421 atagtcctcg gcgtcgccgg ggggatcgcg gcgtacaagg cctgcctgct gctgcgccgc
    18481 ttcactgagg ccggccacga cgtccgggtg gtgcccactg cgagcgcgct cgagttcgtc
    18541 ggcgccacca cctgggcagc cctttccggc catccggtca gcacttcggt gttcgatgaa
    18601 gcggacgcgg ttccgcacgt ccggatcgga cgcgaggccg acctggtggt ggtggccccg
    18661 gccacggccg acctgcttgc ccgcgcggtc accggacgcg ctgacgacct gctgaccaat
    18721 gtgctgctca ccgccggctg cccggtggtg atggcgccgg cgatgcacac cgagatgtgg
    18781 tcgcaccccg ccacccaggc caacgtcgcc acgctgcgtt cgcggggcgt ggtcgtgctg
    18841 cgcccggcgg agggccgcct caccggcgcc gattcgggcc cgggacgcct acccgagccc
    18901 gaggacatcg aagccacctg cctggccgtg ttggcccagg cggacgcggc tggagcgatg
    18961 gcggcgcagg accttcgcgg gctgaatgtg gtgatcagcg ccggcggtac cagggaggcg
    19021 ctcgacccgg tgcgttacgt cgggaacttt tcctcagggc taatgggcat cgcgctcgcc
    19081 cgagccgcgg tgcagcgtgg tgcggacgtg acgttggtcg gcgcgaacct gcatgcggag
    19141 ccgccagccg gtgttcgggt ggtcgaggtg gtctccaccg ccgacctggc cgcggcgatg
    19201 gctgagctgg cgccgtccgc tgatgttgtc gtgatggctg cagcgcctgc cgactacacg
    19261 cccgccgcgc cgagcgacac caagctgaag aagtccggcg acgccggcct gaccgtggag
    19321 ttccgacaaa ccactgatgt gctcgccggc ctggccgccc gccgcccggt ggggcagatc
    19381 gtggtcggct ttgctgccga gaccgcgcgc gacgaggcgg aactgatcga actgggcacc
    19441 gcgaagctgg cccgcaaggg ctgccagctg ctggtgctga acaacgtctc ggacggcgcg
    19501 gtcttcggct cgggcgagaa tgaggtgctg ctgatcgcgc cagtcgggtc gcgtggccgt
    19561 gcagccggaa ccaagacgac agtggcgcat gcgatccttc atgccgccat cgcggagagg
    19621 agtgcagagt gagtcgactg ttcacgtctg aatcggtcac cgaaggtcat ccggacaagt
    19681 tggccgacgc gatctcggac gcggttctcg acgaactgct gagccaggat ccgacgtcgc
    19741 gcgtggcggt ggagacgttg gtcaccaccg gcaccgtggt ggtggccggc gaggtcacca
    19801 ctgaggcgta cgcggatgtg gcccagctgg cccggcagcg gatcctggag atcggctacg
    19861 actccagcga gaagagtttc gacggcaact cctgcggggt gctggtgtcc ttgggcgttc
    19921 agtccccgga tatcgcccag ggtgtcgacc acgccgagga acagcgcgaa gagggctcct
    19981 tcgacgcgct ggacgcccag ggggcaggcg accagggatt gatgttcggt tacgcctgca
    20041 ccgacaccgc gaccctgatg ccgctgccga tcgacatcgc gcaccggctg agcgagcagc
    20101 tcgccaaggt gcgcagggac ggcgacctcg acttcctgct gcctgacggc aagacccagg
    20161 tgaccatcga gtacgccggc gaccggcccg tccgcgtgga caccgtggtg gtctcgaccc
    20221 agcacactgc cgccaccacc cacgaccgca tctcggccga ggtgcgcgcg cgcgtgatcg
    20281 atccggtgct ggcgcgttac gcgatcccgt ccagcgggat gaagacctac gtgaacccca
    20341 ccggcaagtt cgtgatcggc ggaccgatgg gcgatgccgg tgtgaccggg cgcaagatca
    20401 tcgtggacag ctacggcggc atggcccgtc acggcggcgg cgcgttctcc ggcaaggacc
    20461 cctccaaggt ggaccgctcg gccgcgtacg cgatgcggtg ggtggccaag aacgtggtgg
    20521 ccgccggact ggccgaacgc tgcgaggtgc aggtggccta cgccatcggc cgcgcgcacc
    20581 cggttgggtt ctacaccgag tgcttcggta ccgagaaggt gccggtggag cagatcaccg
    20641 ccgcggtgct ggacgtcttc gatctgcggc cggctgcaat catccgtgat cttgacctga
    20701 tccgtccgat ctattcccag gtgacgaact acggccactt cggccgcgag ctcgaaatca
    20761 tgacctggga gcgcaccgat cgagctgacg ccctggccag cgccgtccgc gggtgagccg
    20821 gtagcggcgc gacgcctcag ggttcgacct cggcgtacat gctgagcagg tttccgtccg
    20881 ggtcgctgaa ctcgaagtac tccagcacgc cctcgacctg ctgcaaatca ccggccggta
    20941 ggcccagccg cagcaggcgc tcgcgttcga cttccgcgtc gatcacgccg aaccggacga
    21001 ccggctgggc gccgttgcgc agtacgggct cagcgatcag ctgcagccag atcgggccga
    21061 ctttgaactc gaccacaccc ggggcgggct cgatcccggg ggcgacgaag ccgaagaccg
    21121 tccggtacca ctccaagctg cgtccgatgt cggcgcaggg tagccctacg gtcacactct
    21181 ggatcctcac ccgcgaattc ttgcgcgccg atttggtgta agggagggcg tcggggcggg
    21241 atgagccagc gtgcatcgct cgcgccgggg gcttcacgtt cgaccaactg ggcgagggcc
    21301 tgcgccacga gcgcccgcgg gagatcccgc cagttgtccg gtccgattgt cggtggcgac
    21361 ccctacgatg cccttcatga aacttccggt ggcgagggtt gcggtggatc tgccgctggc
    21421 caacctcgac cggccgttcg actatctggt gccggaggcc cttgacgccg aggctcagcc
    21481 cggggtgagg gtgaaggtgc ggttcgccgg gcagttgcgg gacggcttcc tgctggggcg
    21541 cgcgccggtc ggcgagcggg ccaagctggc cgcgctgcag cgcgtggtgt ccgcggagcc
    21601 ggtgctcgat ccgggcgtga ccgaacttgt gcgagcggtc gcggatcact acgccggtag
    21661 tttcgccgac gtggtgcgcg cggcggtgcc ccctcggcat gcggccaccg agaaggctcc
    21721 gccgccagcc cacccggaac cgcagctggc ggatcgggag ccggtcaccc tgagcgccta
    21781 tccgttcgcc gatcgtttcc ttggcgcgct cgccgaaggg ggccatcccc gcgctgcctg
    21841 gaccgtcgtc cccgccccgg ttccggccgg cgactgggcg tccggactgg tcgaggctgc
    21901 cgaagtcgcg ctcagctccg gacgcggcgc gctgctgttg gtgccggacg cgcgccagct
    21961 gcagcacctg gtcggggtgg cgactgctcg cttcggccgc ggcagctttg ccaccctcgc
    22021 cgcggatgcg ggcccggcgg cgcggtaccg caacttcctc gccgtccgca ggggtggtgt
    22081 ccggctggtg ctcggcaccc gcgcggcagc gttcgcgccg gtgccgaacc ttggtctgat
    22141 cgcgctgtgg gatgacggca acgacgcata cgccgagccg cacgccccgt actggcacgc
    22201 gcgcgaggtg gttgcgctgc gagcacacca ggctgggtgt gcgttgctgc tggccgcgca
    22261 ctcgcggacg gccgagatcc aacagctggt ccagcgaggc tggctggctg agctgcagct
    22321 cggtcccaag ccgacgcgcc gaatcgcggc ggccgtgcgc actgccgggc ccgagaactc
    22381 gcgtgaccca ctggccaccg tggcgcggct gccgcacgac gccttcggcg tggtccgtgc
    22441 cgggctggcc gccgggccgg tgctggtgca ggttccgaga gccgggtacc tgccgatcca
    22501 ggtgtgcgcg ggctgccggg cgacggcacg gtgcccggct tgcggccagg cgctggctga
    22561 tggttccggc agcgggttga gctgcccgct ctgcggaccg ctgctcacgt cctggcggtg
    22621 tcccgattgt ggcgacaccc ggctgcgccg tccggtggtt ggggtcgcgc ggacggccga
    22681 ggagttcggt agggcgttcc ccggcacgaa ggtgctgcag gccagcgccg acaagccgct
    22741 gacgaaggtg ggtgccgagc cggcactggt acttgccacg ccgggcgcgg cgccgccggc
    22801 tgacggtggt tacgctgccg ccgtgctgct ggacgccgag ttgatgctgg ccagggccga
    22861 cctccgcacc ggcgaagagt cactgcgccg gtggctggcg gtcgtgtccg cggtgcgacc
    22921 gggagaggcg ggcggcacgg tgatcgcggt ggccgatccg gggctgcgtg aggtgcagac
    22981 cctgcttcgg ctcgacccgg tcggctacgc cgagcaggaa ctcgccgagc gggtcgcggc
    23041 gggctttccg ccggcggtca agctgatcac ggttgaaggc ccgatgccgg ccgtaaccgc
    23101 ggtgctcgcg gcgctggacc tgtcgccggt ggtgcaggtg ctcggcccgt tcgaagtgcc
    23161 gggacgctgt ggtgtcctcg atgagccggt agcccgggcc accctgcggt gcgggctcgt
    23221 cgaaggggcc gagttggtct cggcggtgcg cgccgtgatg tcggtgcggg cggcgaagaa
    23281 ggaggtgccg gtgcgggtcc gagtcgaccc gcaggtgttc gcctaattcc tgccccgttc
    23341 gggtggtcgc gcttgtcgag gccttggggc gcggtgtcgg tccggcagac tccgacggga
    23401 cgatagggtg ccccgcatgt ccagcctccg catcgcgctc gcttgcctgc cgtttcccga
    23461 gtctccggac gcgtcggtca cggctgcggt ggcggccatc gccgaagcat cggctgccgg
    23521 tgctggcctg gtctgcttcc cggagtgctt cgtgccgggg taccgcgcac tcggcgctcc
    23581 agttccggtg atgcccgcag agtggttggc gacagcccac accacgatcg cggacgccgc
    23641 acgcgcgtcc ggggtagcgg tggtgctggg caccgaacgc tacgtcgggg acgcgctgcg
    23701 catcaccgca ctggtcatcg accccgacgg cgagcagctg ggctggcagg acaaggtgca
    23761 gctcgacccg tccgaggacc acctgtacga gcccgcgccg tccggccggg aggtgttccg
    23821 gatcggcgac ctggtgctgg gggtggtgat ctgccacgaa ggcttccgct acccggagac
    23881 cgtccgcgaa gccgtggcca agggcgcgca actggtggtc tttccgcact actccgagcc
    23941 gttgccgggc cacttccagc cgacgcacta cggtgacccg cggctctcct tccacgagtc
    24001 ggcggtgatg ggccgcgcgg ccgagaacac ctgctgggtg gccggggtga actgtgcggt
    24061 cgccgaagcg ccgacctccg cgatcgcccg tcccgacggc agcctgctgg cctggcggcc
    24121 gcacggcgag ccaggcatcc tggtggccga cctggacctg agcgaggcga cgcatcggat
    24181 ggccaagcga ttccgccctt cggcctaggc tgagccccat gcgtctcgtc ttcgccggaa
    24241 ccccgcaggt ggctgccgac accctcgcgc acctgctcga ccacggcacc cacgaggtcg
    24301 tcgcagtgct gacccggccg gacgccccgc agggtcgtag tgcgcgtccg gtgcccagcc
    24361 cggtggctca actcgcggcc gtccggggaa tcgaggtgct gcgtccggca cgagccggcg
    24421 accccgacct gctggcgcga ctcaccgaac tcgcaccgga ctgctgcccg gtcgtggcct
    24481 acggcggcct gatcccgcag cgactgctgg agctgcccag ccacggctgg gtcaacgtgc
    24541 acttctcgct actgccgcgc tggcgcggcg ccgcaccggt gcagcacgcg atcctcgccg
    24601 gcgacccggt cacgggggtg acggtcttca agctggtccc agagctggac gccggtccgg
    24661 tgttggccac tgccgattac ccgctggggg ccgacgagac gtccggcgaa gccctgagcg
    24721 cgctgcaggc gatcggtgca cagctgctcc tgcagaccct cgacggcatc ggggccggac
    24781 gcatccaggc cgtgccgcag cctgtcgacg acatcaccct cgcccctaag ctgaccgtgc
    24841 ccggggcccg agtcgactgg aaccgaccgg ccgaagagat cgccaggcag gtgcgggcca
    24901 acaacccgtc gccgatggcc tggacgctgt tgcagggcga gcgattccgg gtgctccgag
    24961 cgacgcccgc cgaagccgct gggctcgcgc ccggccaggt acgggccggc aagcgtgagg
    25021 tgctggttgg caccggcgcc ggcacgctgc ggctggagca ggtgcagccg gccgggaagc
    25081 gtccgctcgc ggccgcggac tgggcccgcg gcctacgcgg tgaggcggtc ttcgagtgag
    25141 ccgtcccaaa cggcggcgcg accaagcccg cacggcggcc ttccgggtgt tgcgggcggt
    25201 caccgccgac ggcgcttacg cgaacctcga gttggctcgg cagcgagccg agctgagtgg
    25261 cgccgatgcg acgttcacaa ccgagctggt ctccggcact tgccgggcca tgggcagcta
    25321 cgaccaggtg atcgagcggg cgtccgggcg tccgctgaag tcgttgcagc ctgctgtggt
    25381 ggacgtgctt cggctcggca cccatcagtt gttggcgatg cgggtgccca cgcacgcggc
    25441 gatctcggct tcggtcgatc tcgctgcgct cgaggtgggg gagcgggttg ccggcgtggt
    25501 gaacgcggtg ctgcggaagg tggccgcgcg ggactgggac gactggctgg acccactgtc
    25561 cgaaggactg gacgagtttg gacggttggc gctcgtgcat cagcatccgc gctggatcgt
    25621 cgaagcctgg tcacgggtgc tgcccgcagc ggagttggcg gacgcgttgg ccgccgacaa
    25681 cgtcgcaccg acgccgcacc tggtcgtgcg acccggactt gccgaggttt ccgagcttgg
    25741 cggcgagccc gcccgctggt cgccgtacgg cgcctacgcc ggcggggatc ctgggcagtt
    25801 ggccgcggtc gacgagggtc gcgccggagt gcaggacgag ggttcgcagc tcgtcgcact
    25861 ggcgctgtcg cgggtcgacg cgcccgatgg ctggtggctg gacctgtgcg ctgggcccgg
    25921 tggcaagtcc gccctgctgg ccggcttggc tcgcgagcag ggcggctcgc tggtcgccgc
    25981 cgaactgcag gaacaccgcg cccgcctggt cgccgacaac ctgcgggcat atccagatcc
    26041 gaagccggtt tgtctggtgg cggacgggac ccgtccggcc tggcgatccg gggggttcag
    26101 caaggtactg gcggatgtgc cctgcacagg gctgggcgcg ctgcgccggc gtccggagag
    26161 tcggtggcgg cggcgtcccg aggacctgcc ggggctgatc gagctgcagg ggaagctact
    26221 ggcgtccgcc atcgcggcaa ctgcgcccgg cggtgtggtc gcctacgtca cctgttcgcc
    26281 ccacccggac gagaccgccg ccgtggtagc tggggccagc ggtgtcgtga cgctgcgcgc
    26341 cgccgactat ctgccggaag tgccaaattg tgcgtccggt gacttcgtcc agctctggcc
    26401 acaccgccac ggcaccgacg cgatgttcct ggcgctgctt cgccgcagca cctagccagc
    26461 catcaaccgc tcggcgagcc ccacagctgc gcaggctgga cagttctggc ccgtcctggg
    26521 acctagcggt ccagtttgcg gacgggtgga gcggggcccg gcggcagata gggtttggcc
    26581 cgtgggtatc cgaatcacgc cgagcatcct caacgccgaa ctgggcggcc tcaacgccga
    26641 gatcgcccgg gttccgagcg ccgatggcgt ccatcttgat gtcatggaca accatttcgt
    26701 gccgaacctg accttcgggg tgccgatggt ggagagcatc cggcgcaaca cgaccaagtt
    26761 cctggacgtc cacctgatga tcgctcaggc cgatcgctgg gcacctgcct acgccgaagc
    26821 gggcgccgag tcggtgacgt tccacatcga agcctcggac gcgccggtga agctcgcccg
    26881 cgaactgcgc ggccacggtg cgcgcgcgtc catggcgctg cgtcccgcca ctccgatcga
    26941 gccctacgaa gacctccttg gagagctcga catggtgctg ctgatgaccg tggagcctgg
    27001 cttcggcggc cagaagttcc tcgacctggt gctgcccaag atccgacgga cgcggaagct
    27061 cttggacgca cggggcggcg acatctggct gcaggtcgat gggggagtgt ccgcagagac
    27121 cattgagcgt tgcgtcgaag ccggcgcgga caccttcgtg gcaggctcgg cagtgttcag
    27181 ctcagacgat ccggacgccg tggtacgcgc gctcaaggct caggcggagg gcgtggccag
    27241 ctgattctgc ggcccgccag tcggtgtggg gtcggcggag atcccggcct tcgccgggat
    27301 gacggtaagg gcttgggctt acggtgggcg gcttgggctg acagtggcgg cctggggcga
    27361 cagtgatccc tcgggtgtag ctcagccgcg gcggcgatcc cggcgacgca ggtagcgttc
    27421 gaactcggct gcgatcgcat cgcccgaagg cgccggggtg gcctcgtcgc gacgctcctc
    27481 cagcgcggcg atgtagccgg cgatgtccgg atcgtcggcc accagctcgt tcacctgctc
    27541 cacccaggac tctgccgcct ccggcagctc acccgggtcg agcggcaggt tgagcacgtc
    27601 ctcgagccgg gtcatcaggg ccagcgtggc tttcggattc ggcggctcgg caacgtagtg
    27661 cggcaccgag gcccacatcg agaccaccgg catcccggcc acggagcact cctgtgacag
    27721 cacgccgacg atgccggttg ggccttcgta gtccgagatc tccagcccga gttctgcggc
    27781 caactcggcg tcgctggcag tcccgctgac cggcaccgga cgggtgtgcg gcgcgtcggt
    27841 cagcagcgcg cccagcacca ccaccatctc cggccgcacc gagcgcaaca gcgacatcag
    27901 ccggttcagg tactcctgcc agcgatagtt gggctcgggg ccaccgatca ggaccaggtc
    27961 acgttcgggc aggttggcca ccagcacctc ggtggtcggc cactcgatcg tccgcttgcc
    28021 gtcgatcagc cgggtggtgg gacggttcac ctggaagtcg tagaactcct ccgggtcgat
    28081 cgcgaactcg agttcggcgc ggtaagtgtc gaccagatga tcgatcacgc cgctcgctgc
    28141 gtcgccggcg tcgttccagc cgccgaaagc cgcgatcacg gctgggttgc gcaaacccct
    28201 gagcccacga gcctctgcca tggcgaccac aataggcgga ctcgcctatt ctgctcccca
    28261 tgacccgcga cttgcgctcg gccctgacca cccgcgtcct agtggcggac ggtggtatgg
    28321 gcacgatgct gcagggctac gacctgacca tggacgattt ccagggtctg gagggctgca
    28381 acgaggtgct gaacgtcacc cgtccagatg ttgtgggggc gattcacgcc gcctacctgg
    28441 acgccggtgc cgacctggtc gaaaccaaca cgttcggggc gaatctctcc gccttgggcg
    28501 agtatgacgc caccgagcgg atcgccgaac ttgccgaagc gggcgcccga atcgcccgcg
    28561 ccgctgcgga cgcggcgagc acccccgatc gttcccgcta tgtgctcggc agcatcgggc
    28621 cgggcaccaa gctgcccagc ctcggccacc tgccctatgc gacccttcgg gacgcgtacc
    28681 aggtgcaggc cgaggcgatg ctcgccggtg gcatcgacgg gttccagatc gagaccagcc
    28741 aggacctgct gcaggccaag gccgcggtga acggcgcccg gcgggctcgc gctgcgctcg
    28801 gcgtcaatgt accgatcttc gtcaacgtga ccgtggaagc ctccggaacg atgctgcttg
    28861 gcagtgagat cggcgctgcg ctgaccgcgc tcgagccact ggagatcgac gccatcggcc
    28921 tcaactgtgg cactggtccg accgagatga gcgagcatct gcggcacctg tcccggcacg
    28981 cgcggatccc gttggtctgc atgcctaacg ccggcctgcc cgagctcacg ccggacggcg
    29041 cccgctatcc gatgggcccc gaagagttcg tcggctacgt ggcccagtac acctccgagt
    29101 tcggcctggg gctggtgggc ggctgctgcg gtaccactcc ggcgcatatc ggcgccctcg
    29161 ccgcagccgt ggccgggcga ccgcccgcgc cgcgcgaccc cgtccgcgtc tcggccgttt
    29221 cctcgctgta cagcgaagtg ccgctgcgcc aggagctcag ctacctctcc attggcgagc
    29281 gcacgaacgc caacggttcc aaggccttcc gcgaggccat gctggccgag aactgggccg
    29341 attgcctcga catcgccaaa cagcagagcc gaggcggcgc ccatctcctc gatctgtgcg
    29401 ttgactacgt cggacgcgac ggtgcagccg acatggccga actcgccttc cggctcggca
    29461 ccggcgtgtc cctgccgatc atgctcgact ccaccgagcc cgccgtgctg caggcgggcc
    29521 tggagcggct cggcggacgc tgcgtgatca actcggtgaa cttcgaggac ggcgacgggc
    29581 ccgactctcg cttcgccaag gcgatgccgc tggtgaagga gcacggcacc gcggtggtgg
    29641 cactgaccat cgacgaggag ggccaggccc gcaccgccga gtggaaggtg cgcgtcgccg
    29701 accgcctgat cgccgacctc accaccaagt gggggctcga gatcggcgac atcatcgtcg
    29761 actgcctcac cttcccgatc gcaaccggac aggaggagac ccgccgggac gcgctcgaga
    29821 cgatcgaggc gattcgtcag gtcacctcga aatacccggg aatccacacc acgctgggcg
    29881 tttccaacgt ctccttcggg ctcaacccgg ccgcccgaca ggtgctcaac tcggtattcc
    29941 tgcacgaatg cgtcgaggcg ggcctggatt ccggcatcgt cccggccgcg cggatcgtgc
    30001 cgatcgcccg catccccgaa gaacagcggc aggtagccga agacttggtc tacgaccggc
    30061 gcacccccga ctacgacccg ctgaccagat tcctcgaact gttcgagggg gtaaccacca
    30121 ccagcaccaa ggaggcccgc gcagccgaac tggccgcgct accggtcacc gaacggctcc
    30181 agcggcgaat cgtggacggt gacgccaagg gcctgggcaa cgatctcgac gacgcgctcg
    30241 ctgccaagga cgccctcgcg atcatcaacg acgacctcct ggccgggatg aaggtggtcg
    30301 gtgagctgtt cggctcgggc cagatgcagc tgccattcgt cctccagtcg gccgaggtga
    30361 tgaagatggc ggtcgcgcac ctcgaaccgc acctcgacaa ggcggacgcg gccggcaagg
    30421 gcaccctggt gatcgccacg gtgaagggcg acgtgcacga catcggcaag aacctggtgg
    30481 acatcatcct caccaacaac ggctacacgg tggtcaacct cggcatcaag cagccggtca
    30541 gtgcgatcat cgaagccgcc ctggtgcaca acgccgatgc gatcggcatg tccggcctgc
    30601 tggtgaagtc gaccgtggtg atgaaggaca acctgcttga actgaacaat cgcgggctcg
    30661 gcgggttccc ggtgctgctc ggcggtgcag cgctcacccg ggcgttcgtg gaggaagacc
    30721 tcaacgacct ctaccagggt gaggtgcgct atgcccgcga cgccttcgag gggctggcat
    30781 tgatggagaa ggtgatggcg gtgaagtccg gcgtccccgg tgccgcactg cccgagccac
    30841 gccgccgccg ggtggtcgcc aagccgcgag cgaacgaccc ggaggcggtt gattcgaccg
    30901 aacgctcgga cgtcgcccgc gatcaggatg tgccgacgcc gccgttctgg ggcagccgtg
    30961 tggtgaaggg cctcaagctc gccgaagtcg ccggctggct ggacgagcgc gccaccttca
    31021 tgggccagtg ggggctgcgc ggcagctccg gcggcccgac ctacgaggaa ctcgtcgaga
    31081 ccgaaggccg gccacggctg cgacactggc tcgaccgcgt ccagaccgag gcactcgcgg
    31141 aaccggcggt cgtctatggc tactggccct gccattccga aggcaacaca ctgatcctcg
    31201 gcgacccgga cgacccggcc cgagaagtca cccgattcat ctggccgcgg cagtcccgcg
    31261 accggcggct ctgcctggcc gacttcttcc gggacgcgga cgaggccgcc gaacgcggcc
    31321 ccgacgtgat cgcgctgcaa ctggtgacca tgggctcggc ggtcgcccag gccacgacca
    31381 agctgttcga ggcggacgcg tatcgcgact acctcgaatt gcacggactg tcggtgcaac
    31441 tcaccgaagc cctggccgag tactggcacg cccgcgtccg cgccgaactg gggctcggcg
    31501 gcgacggcga ggtcgatgcc atgatccgcg accaggccta tcgcggctcg cgctactcct
    31561 tcggctaccc ggcctgcccc gacctggccg atcgcgcgaa gctggtgacg ttgctgcgtc
    31621 cggagcggat cggggtgacc ttgtccgagg aactgcagct acaccctgag cagtcgacgg
    31681 acgcgatcgt cgtgcaccat ccggaggcca agtacttcaa tgcccgctga tcgcctccgc
    31741 aagctggcca cagccctcgt cctcgccgtg gcgctggccg gctgcacgcc gaccgttgtc
    31801 gagccgacgc agagcccggc tgtgacgccg accgcgcgtc cgttcacgct gatcacgagc
    31861 gacccggtca cgacggctga cccggtgatc gcggtcgggg acaccgactc gctcctggtg
    31921 aacagcgtct atcaacggct gatgctcgtc ctgcccggca gcggggagtt gaaacccgat
    31981 ctggcgaccg actgcctgtt cacgtcggag acggtgtacg agtgcacgct gccggaggag
    32041 ttgaccttcc acaacggcaa cgtcctcgac tcctccgacg tgaagttcag catccagcgc
    32101 gccctgcgaa tgggtgcggc caacacatcc atcggtttgc tctcggcgct caagcgaatc
    32161 gaaacgccgg acgcccagac ggtgaagttc tacctctctt gggccgacaa ccagttcggc
    32221 tacgcgctgg ccgggcaggc tgccagcatc gtcgacgagc aggtctacga cccggacgcg
    32281 gcgctgccgc tcgatgtgca gccagtgggg tctggtcagt tccaggtgtt cgagatcgcc
    32341 gcagagcagg tcgacttcac caagttcgaa acctacttcg ggccgcgcgc cggcatcctg
    32401 gacgcagtgc gtctccaggt tgtgcccgac tcggtggccg ccgaagccgc gatcgccgac
    32461 gggaccgctg acgtggtctg gcgcagcgtc gacggtgctg cgctgcagcg gctgaccgac
    32521 gagatcgccg ccagtccgga caagaccaca gcatccggct tcacctccta ccacctgccc
    32581 ggcgcccgcg tcacgcagct gatctggaac gccaactcga agctgcgcga gaacaacgac
    32641 ctgaggctgg gggtagcgaa ggcactgcag ggcgaccgca cgctggactc gatcgtgccg
    32701 gtcggggtgg tcgaccatgc cgcatccttc gcgctcggcg gtcggccgaa gctgccggag
    32761 ctaaagggga agcggatcaa gctcacgctc gggtacaccg ccaccgcccc cggtcacgcc
    32821 gaccaggcgc ggctgctgcg cgatcgcatc gagagcatgg acaaggtgag tgttcgcctg
    32881 gtgaccaccg gtgatgccga cctgtggttg accgaccgtc cggcctgggt caacaacgcc
    32941 accggctggc tgcagcgcta tctcgccgaa ccgctggccg gcagcagcga gaagctggac
    33001 gaactggtgc agcggtcgcg caccacaacc ggggccgcgc ggacggtggc cttcaccgag
    33061 ctgcagcagc aggctgcggc cgacaacacc gtgctgccgg tgtcgcaggg ggatggcatc
    33121 ctgctgctcg gcaagggtgt caccctggtg ggcgacgcct tcggcagcgg gcagcaacta
    33181 ggactctggg ggatcagcca tgcctgaact tgagccgctc agcggcgtcc gcacgggcgt
    33241 gctgcgtgcc ggagaacgaa tcactctcag cgatcccaag ggacgtaggc actcagtcgt
    33301 gctggcggac ggaggcacct tccacaccac caagggcggg atcagccacg acgagctgat
    33361 cggacgtccc gagggcagcg tcgtgcactc ggccggcggt gtgggctatc tcgctttccg
    33421 cccgctgctg gaggagttca ccgtgacgat gccgcgtggc gccgccgtgg tgtacccgaa
    33481 ggacgcggcg cacattttgg ttcagaccga catcttcccc ggcgcccggg tgctggaggc
    33541 cggtgccggc tcgggcgcgc tgagcatctt catgctccgg gccatcggcc cgaccggacg
    33601 cctgtactcc tatgagcggc gcgccgagtt cgcgaagatc gccgagcaga acgtgaccaa
    33661 gttcttcggg gagccgcatc cggcctggac gttgcgggtc ggcgacctgg tcacggacat
    33721 cggcgacgag ccgatcgacc gggcggtgct ggacatgctg gccccctggg agtgcatcga
    33781 cgtggtggcg aacgtgctgg tggccggcgg tttgctgtgc tgctacgtgg ctaccaccac
    33841 gcagttgagc cgcaccatgg agacgctgcg ggcgcacggt gggttcactg agccgtcggc
    33901 ccgggagtac tcggcgcggg aatggcatgc cgagggcctg gcggtgcgtc ccggtcacca
    33961 gacgtccggg cacactgggt tcctgatctt cacccgccgg ttggcgcctg gggcggttgc
    34021 cccgctgcgt cgccggcgtc ccgctcccgg ggcgtacggg ccggactact ccggtccgcg
    34081 tccgcgcggc actgagaccg agtcggcgga tgcctgagcc agccgaactg gcccgtcggc
    34141 tagaggtaac cgagcgtcgc ctcgcggatg ctcgctcgga cgcgcgcacc ctcgccgcga
    34201 ccaaccagaa gctcaacgcc accctggtca ccgcccgcga tgcattggac tcgctgcgag
    34261 ccgaggtggc ggcgttgggc cagccgccga acacgttcgg ctacgtggtc ggcaccggcg
    34321 acggcacgat cgacgtcgcg accaacggac gctggctgcg ctgcgccctc agcccggacc
    34381 tggccgacca acgtttcgtc gtcggcgacc gggtcctgct caacgaggct cagctggtgg
    34441 ttggcatcgc caacgaaccc ggggtggggg agaccgcccg cctggtctcc accctgcctg
    34501 agtcaccctt gcgactgctg gtcaccacgg cgaccggcga agagacggtc gtgctggcgt
    34561 ccacgagcct taacccagcg gaactccgcc cgggcacccc gctgctggtc gaccggcgcg
    34621 ccgggctggc gctcgtggtc ctgccgaaca acgaggtggc tgacctgacg ctcgaggacg
    34681 tcccggacgt cagctatgcc gacatcggcg ggctcagcga ccagatcgag cagatccggg
    34741 acgccgtcga actgccgttc cgtcaccggg agttgttcgc cgagttcggc ctgcgtccgc
    34801 cgaagggcgt gttgctgtac gggccgccgg gctgtggcaa gacgatgatc gcgaaggctg
    34861 tggcgaactc ggcgggcagc cacttcctga acatcaaggg tcccgaactg ctgaacaagt
    34921 acgtcgggga gaccgaacgc cagatccgcg cgatcttcac ccgggcgaag gagcacgcga
    34981 cgtccggacg cccagtgatc gtgttcttcg acgagatgga cgccctgttc cgcacccgtg
    35041 gtagcggggt ctcctcggac gtggaggcaa cggtcgtgcc gcagttgctt gccgagatcg
    35101 acggtgtcga ggacctggcg aatgtgatcg tgatcggtgc cacgaaccgc gaggacctga
    35161 tcgacccggc gatcctgcgt cccggacggc tggacgtgaa gatcaagctc gaccgccccg
    35221 atcgatcggc cgcggcggag atcctggccc gttatctgac tgaagcaaca ccgctgaatc
    35281 cgggtgaggc cgcccttcga caagctcagg gatcgttggc tgggactcag gggtcgttgg
    35341 ctgagcctgt cgaagcccgc accgacttcc gcgaacaact catcgacgcg atcgtggatc
    35401 gcctcttcgc ccgcacggac gccaaccgct tcatcgaggt gacctacgcc ggcggcgata
    35461 aggagacgct gtacgtgggc gacttcgtgt ccggcgcgat gctggccggc gtggtcgacc
    35521 gggccaagaa ggccgcgatc aaggaactga tcaacggcgg cgagcgcggc gtccggctcg
    35581 cccacgtgga agctgcctgc gccgccgaaa tcggtgagaa cgaggagctc ccgaacacca
    35641 ccaaccccga cgactgggcg cgagtatccg gccgcaaggg cgagcggatc gtcttcctgc
    35701 gtactctggt cggcgcccgg gccctgaacc cgtgaccagt cgatcgacca cctcgccacc
    35761 accgttccgc tcggtcttcg ggatcgaaac cgagtacggc atccacgcac cgaccgctcc
    35821 ggaagtccat cccagcgtgc tcagcgcggc cgtggtcggc gcctaccccg gcgtcgcgac
    35881 cggtagccag ctggattggg tcgagccgat cccgatcgag gatgcccaca accgcgtcct
    35941 cgccaacggg gcccggctct acgtggatca cgcccacccc gagtacagcg ggccggaagt
    36001 caccacggcc gcggacgcag tcgcctacga cctggccggt gacgctgcgg tggtcgctgg
    36061 cgccaaggcc gcctcggccc agctgggttt cgacatcgcg atctacaaga acaacaccga
    36121 ttcgcacggt gcctcctatg gctgccacga gaaccatctg ctgccgcgct cggtgccgtg
    36181 gacgcggatc gcgtccgtcc tgcccgcgtt cctggtctcg cggacggtgc tgatcggcgc
    36241 cggacgggtg ggtctcggcc agcacgggga gcgtgccggc tttcagctca gtcagcgcgc
    36301 ggacttcttc gagaccctcg aaggcattca gaccacgaag cgtcgcccaa tcctgaacac
    36361 ccgggacgag ccgcacgcgc cgccgagtcg gtatcggcgc ctgcacgtca tcaccgcgga
    36421 cgcgaatcgc aaccagttcg ccacctggct gaaggtgggc agcctcgcca gcttgctagc
    36481 ggcgctcgtc cacgatgcgg tgccggtcat caccctcacc catccagtcg cggcgttcca
    36541 agcgatcagt cacgacccgt ccctcaccca ggccgttgag acttcgcagg gacggatgac
    36601 cgcgctcgac ctgcaggaag cgttgctgac cgccgtcctg acacacgccg aacgtgcacc
    36661 cgagatgcac gaggcgtccg tcctggcggg gtggacgcag accctggccg acctgcgcac
    36721 cgacccgtcg agctgcgcgg accggctgga ttgggtggcc aaatacgagc tgatgcgggc
    36781 tttcgccgac cggcacgacc tttcgtggga agacctgcgg ctcgccgcac tcgacctggc
    36841 ctgggctcga ctggacgccc ccggaccgtt cgatgcgctg acccgcgcgg gacatcaacg
    36901 ggccgtggtg agcccggccg acgtcgccgg tgcgagtgct tcgccacccg ccgatacccg
    36961 ggcctggctg cgggggacgc tcgtgggtcg catccccgat cgcgtgctgg ctgcttcctg
    37021 ggactcgatg acggtcctgg acgcccacgg ttcgcgccgg caccttaatc tggccgaacc
    37081 gctgcggcac accagggcgg aggtcggcga gccgacggac gcggcaacgg tgtcggcact
    37141 gctgcgtcag ttggggtgag taggctgtgc gctcgacgag tcaggaggac tggtgaccga
    37201 gcgagtgcat gccgaacagg tgcgagcgcg taccgaagac acggttgagg agaccgtgaa
    37261 gacttcgcgt gccatcgact attcggcagt gctcgacgag atcgacgcag tgctggaagc
    37321 ggacgccgag gagtacgtcc gctcgttcgt gcagaagggc gggcagtgaa cgccccggtg
    37381 ctgtcgcccg cgctggtcat cggaaccgga ctggttggcg cctcggttgc gcacgcgctc
    37441 acccgggccg gggtgaccgt ccacctgtcg gacgcggtga ccagccacgc tcgggtggcg
    37501 gcttcgcgcg gtgccggcac gactacgccg ccggtggctg ctgacgtgca gttggtcgtg
    37561 gtcgcggtgc ctccacacgc cttggcgaag gtgattgcga agtcgctgcg gaccttcccc
    37621 aacgcgaccg tgaccgacgt cggctcggtt aagggcacgg tgctggccgc gcttcgggca
    37681 acggacgccg atctatcgcg ctatgtcggc tcacacccga tggcggggtc gcagcgcgtc
    37741 ggtccgctga ccgcgaacgc ggacctgttc attgatcgca cctgggtggt gaccccgcac
    37801 gacacgtcgg ctgcgcacgc cgtgctggcg gtgcaggaac tcgcgacgct ctgtggcgcg
    37861 cgccgggtga cgatgggcgc agcccaccac gacgaggcgg tcgcccaggt ttcgcacctg
    37921 ccgcacctca tgtcggtgct ggttgccggg acgctgaccc ggctgcccgc cgagcacctt
    37981 cggctggccg gacagggggt gcgggacgtg acccgcatcg ccggttcgga cccgaaactg
    38041 tggcagcaga tcatcggcgc caactcggca gcgatccggc gcgagctggt ggagatccag
    38101 gccgaactgg ccgacatgct ggctgcgctg gacgcgggtg acctcgccga ggtgctggaa
    38161 cgcggcctgg ccggcacgaa ggcactgccg ggtaagcatg gcctcagccc caccaactat
    38221 gcgcaggtgg tcgtggagat cccggacgcc cccggcgcgc tggcgcggtt gttccgcgac
    38281 atcgagttgg ccggagtgaa cgtggaggac gtctccatcg accacgacct ggagcgcgag
    38341 gtcggctacc tgtcggtgga cgttgccccc gatcgggcgc caggcctggc cgaagccatg
    38401 actgcagccg gctggaccgt ccgcgaccgc gagccccagc catgaccgct atttcgaagg
    38461 agacaacatg accgaacgcc tggtgatcgc catcgacggc ccgagcggct cgggtaagtc
    38521 gtccacggcg aagggcgtgg cgtccgccct cggattggct tacctcgaca ccggcgcgat
    38581 gtaccgcgcg gtgacctgcg cctacctgga cgcaggcgtg acgccggcca acgaggccgg
    38641 cgtgatcgac gcgaccacgt ccgcacgttt ggagatcagc acggacgcgt ccgaccagtg
    38701 ggtgcgggtg aatggcgcgg acgtgaccca gcgaatccgc gagccggaga tctcggcgaa
    38761 cgtgagcgcc gtctcgacgg tcgcggcgtg ccgtgccgac ctggtggcgc ggcagcgggc
    38821 gatcatcgac gccgccgagg tgggcatcgt cgccgaggga cgggacatca ccacggtcgt
    38881 ggcgccggac gcgcatgtcc gcctgctgct gactgccgat ccggaggcgc gaatggcccg
    38941 ccgcaaggcc gaactgggcg agaaggtgga cgacacccag ctggccgacc agatcctgcg
    39001 ccgcgactcc gacgactcca agctggtgaa cttcaccacg gccgccgacg gtgtcaccca
    39061 tctggacagc acgttcctcg acctggagga aacgatccag gcggttctcg acctggtccg
    39121 ccgcacgatc aacgcttaac cggggcgagc ccggagtgcc ccaaaacggt ttctgagccc
    39181 tgtctcacaa acggtccctg agcctgtccc acccccatcc cggcgaaggc cgggatctct
    39241 tcagcgcgat cgacgcagtc cggctgagca accagcgcgt tcccgaaacg gtccctgagg
    39301 gcgagcgcca gcgagcgccc acgttaggcc gctgagaccc cgcgaaatcc gtccgcccga
    39361 agaactgcgg tagcctgatg ggtcgcacca gaagggactc cgaatgaccg aactgaacga
    39421 gccggacgca gttaagcctg tcgtcgcggt cgtcggccgt cccaacgtgg gcaagtcgat
    39481 gctggtgaac cggatcatcg gccgccgcga ggcggtcgtg caggacctgc ccggcgtgac
    39541 ccgtgaccgg gtgagctacg acgccgagtg gaacggacgc gagttcgtga tcgtcgacac
    39601 cggcggctgg gcgccagacg cgaagggcat ggccgcgcag atcgcggagc aggccgaact
    39661 ggcgatcgcc gcgtccgatg tggtgctgtt cgtggtggac gcgacggtcg gcacgcagga
    39721 tgtggacgag gccgtggtgg aggtgctcag gcgcagcaag aagccagtgg tgctggccgc
    39781 gaacaaggtg gacgacacgc gccaggaagc ggacgccgcc ggcatgtgga acctggggct
    39841 gggggagccg cacccggtgt cggcgatgca cgggcgcggg acgggcgatc tgctggacgc
    39901 gctgctggag gtgttgccgg acgcaccgcg gatgggcgag ccggaggtcg gcggaccgcg
    39961 tcgggtggcg atcatcgggc gtccgaacgt ggggaagtcg tcgctgctga accaggtggc
    40021 gaactcgcgg cgcgcggtgg tcgacaatgt ggcgggaacg acggtcgacc ccgtggacga
    40081 gctggtcgag gtcgggggag tgatctaccg gttcatcgac acggcggggc tgcggcgccg
    40141 ggtgaaggaa gcgtcggggc acgagtacta cgcgtcgttg cggacgcagg gggcgattga
    40201 gcgcgccgag gtttgcgtgg tggtgctgga ttcgtccgag acgatcagcg accaggacct
    40261 gcggatcctg acgatggtcg aggaggccgg caaggccctg gtgatcgcgt acaacaagtg
    40321 ggatttgacc gacgaggagc ggcggcacta cttgagccgc gagatcgagc aggacatccg
    40381 ggcgttcgcg tgggcgccga gcgtgaacat ctcggcgctg accggtcgta atgtggacaa
    40441 gctgtccaag gcgatcgagg aagcgctgga aggttgggag acccgggtgt cgaccggcaa
    40501 gctgaacgcg ttccttggcc ggctggcggc cgcgcatccg cacccggtgc gcggtggaaa
    40561 gcagccacgg atcctgttcg ggacgcaggc gcacaactgt ccgccgacgt tcgcgctgtt
    40621 cacctccggg cagttcgacc cgcagtacgt ccgcttcgtg gagcgccgtc tgcgcgagga
    40681 cttcggcttc cgaggttcgc ctgttcacgt ggaagtacga gcccgagaga agcgcaagaa
    40741 ccgctgaccc ttgttcactg tcatcccggt gtaggccggg atctcagccg ggcctacacc
    40801 ttctccgtca tcccggcgta ggccgggatc tctcagcgca accggtggat ctgggccgag
    40861 gatcgtcacg agcggccgac gcctccgaac gctgttcgaa ctcccacttg atcgaccctg
    40921 cccgacaccc tcagaccgtc cgcggcacct tcccgaaggc ctgcagcacg cgacacatga
    40981 gcttgtgccc gccgtccgcg gcagggtcga acatcagcgg acagccgccc tcgatcacgg
    41041 tgatgccgtg cgcgcgtccc caagcggcgg cgtcggcgtc cacgctgcct gcgtccatcg
    41101 agcgatgcat ccagacccgg gtgatgccga gcgccgctgc ttgctccatg gtcgcttgcg
    41161 cgtgctcggg acgcgtgccg atgacgaccg cctccacccc tccgggtacc gaggccaagt
    41221 ccgggtaggc ggacactccg gcaacggatt cagcgttcgg gttgatgggg aagacgtcga
    41281 aaccccgctc cacgagccgc tggtggacga cattgctgcc tcacctcttc cggtggacgc
    41341 ggaccgcgcc cgggtcgatg aacatcatcg tggggtgagt tccaccgtag cgaatgccga
    41401 ggttgtggcc gtcgccggga tcggcggggc tggtcgggtc gcgcaccggt tgcaccgcag
    41461 agaccgcgcc ggacgcgctc acgctgatca gtccagccac ggtctggttg gtgtccgggg
    41521 tgagttcgac gaaaccaacg atcagcaccc acaagcggcc gttagcgctc atcacggctt
    41581 gaacactcgg atgaacggtc ttgtccgcca gcccggacgc cttcaggtcg agccgaaggt
    41641 tcacttcggg acgaagtacc cgaaggtagc cgtcgttgac gctgagctgg aacttgcgtc
    41701 cgccctgtcc ccggaaccag gtgaccgcgg tgatcttgcc ggacaccggc gcgacgcgaa
    41761 ccttctt
//
LOCUS       DAOPRQ010000012        39790 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_769_length_39790_cov_12.344860, whole genome shotgun sequence.
ACCESSION   DAOPRQ010000012 DAOPRQ010000000
VERSION     DAOPRQ010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 39790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39790
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_769_length_39790_cov_12.344860"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            106..1533
                     /gene="murF"
                     /locus_tag="PKJ61_02590"
     CDS             106..1533
                     /gene="murF"
                     /locus_tag="PKJ61_02590"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015070010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HOC12748.1"
                     /translation="MRERNSRELAGLLGAELTGDGDVPIGPDVVIDSRAVTPGCLFVA
                     MPGVRVDGHDFAAAAVEAGAAAVLVSREVPVGVPQLVVADPALALARLATAVVADATA
                     SGLVTIGITGSSGKTSTKDLLAQVLATAGETVAPVGSFNNEIGVPLTATRVGPTTRYL
                     VSEMGARGIGHIGWLCRIVPPQVGVVVNIGHAHLGEFGSVETIAQAKGELVEALPASG
                     WAVLNADDEQVRTMAGRTSARLAAFGVDAEPDFGELRVWASDVQADLLERARFRLHAA
                     GAAFGEAVVELQVTGRHQVGNALAAAAVALTQGLSLAQVATGLTQAVARSHWRMELTA
                     RPDGLLVVNDAYNANPDSMQAALQTLACLRQPDGRLLAVLGDMLELGDGAAAAHRDIG
                     ALAARLGVDHLIAVGDFAADLVAGASADGLHAQIAADREQAAELAAAWLRPPDVVLVK
                     ASRGLALEHVAELLLADLTGTPGEG"
     gene            1541..2605
                     /gene="mraY"
                     /locus_tag="PKJ61_02595"
     CDS             1541..2605
                     /gene="mraY"
                     /locus_tag="PKJ61_02595"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020387374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOC12749.1"
                     /translation="MRSILLAGGVGMLGTLLGTRYAIRFLVKAGYGQFIRDDGPTSHH
                     TKRGTPTMGGIVLILSVLLAYAVAHLATGTPPSATSLLALGLFAGLGLVGFIDDWIKI
                     SKARSLGLHASVKFGLQTAVAVTFGILAFQFPDARGLTPASTYISFLRDLPWFALPMW
                     AAVIWIVFLVAAWSNAANLTDGLDGLATGAAAMVFAAYTIVNIWQYNQSCALASSGPQ
                     CYEVRNPYDLAVVSIALAGACFGFLWWNASPAKIFMGDTGSLAIGGALASLSILTRTE
                     LLMAIVAGLFVIITASVVLQIGWFKISGGKRLFKMAPLQHHFELLGWKEVTITIRFWI
                     ICGLFVAVGLGLFYGEWVVG"
     gene            2602..4077
                     /gene="murD"
                     /locus_tag="PKJ61_02600"
     CDS             2602..4077
                     /gene="murD"
                     /locus_tag="PKJ61_02600"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016456602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HOC12750.1"
                     /translation="MKPWLAGADRLSPWSQAKVVVAGLGTSGFAAADGLLDLGAQVLV
                     LDDSDSADNAEKAKVLELLDAEVRLGPGATAVLPDDTDLVVTSPGWRPSAPLLAQAAL
                     RGVPIWGEVELAWRMSMPDRQVPWLAVTGTNGKTTTVGMLESMLTAAGLRTSAVGNVG
                     RPIVEAVLDEVSYDVLAVELSSFQLHWTNSLSLHSAAVLNLAPDHLEWYADRAGWPDG
                     VTDPMAAYVADKARIYERVQQSCVYNVADDATMHLVEAAEVVEGARAIGFTLGIPGPS
                     MLGVVDDVLVDRAFIAQRRDSAVEIAKLSDVAPYAPHNVENALAAAALARSFGVPPTA
                     VAQGLQRLRLGDHRIQTVAEIGGVRYIDDSKATNPHAAAASLGAFEHVVWVAGGQAKG
                     TTFDDLVTAVAPRLRAAVVFGVDRDVIAAALARHAPEVRVFVIEDRDHGAMQQAVEWA
                     ATQAVPGDVVLLAPGCASKDMFSDYAARGDAFAAAVSTLTG"
     gene            4228..5370
                     /gene="ftsW"
                     /locus_tag="PKJ61_02605"
     CDS             4228..5370
                     /gene="ftsW"
                     /locus_tag="PKJ61_02605"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HOC12751.1"
                     /translation="MLVPAVLLLALGMIMVLSASSVYAFVRWGDAYYFVKRQAIFLVV
                     GGVAAFVLAARSTKQLKVLGWILLIAAVGLQILTYTPLGYSNNGNTNWVQFGTSLFRI
                     QPSEFAKLAIVLWGADIFARKRKQLGDPRHLLIPFLPASLILIGLVVLQSDLGTGMIM
                     GALVLAVLWYVGASWKVLGAIVGSVAAAVGLLVVTSPYRMNRILGFFNQEVDQLGVNH
                     QPIKAIFALASGGWWGLGLGASRQKWGGLVESHTDYVLAVIGEELGLIGTLSVLILFL
                     ILGYAGFRIAMRSDIRFCRYAAAGVTSWFMIQGLVNIAVVLRMIPVIGVPLPLLSYGG
                     SALMANLMALGVLLACARQEPEYRAFARRRRSRKERALVTTAAGRA"
     gene            5367..6470
                     /locus_tag="PKJ61_02610"
     CDS             5367..6470
                     /locus_tag="PKJ61_02610"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015582992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HOC12752.1"
                     /translation="MNAVRIVLAGGGTAGHTSPLIATANSLAELAEVDVVCVGTPKGL
                     ESRVIPDAGLRLQLIPPVPLPRRITPELFAVPGRLWRAVREAKAILVAHGAEVAVGFG
                     GYVSLPVYLAARQAKVPLVLHEGNYVPGLANRIAARFTPHVVTTFAETALPHAQHLGL
                     PVREAIATLDRDARRPAAREAFGLPADGRVLLVSGGSQGARSLNQATLAARERLLAAG
                     ISVLHVLGAANLTAEDVPVTHPNTGAVYRPVGYVDEMANAYAAADLMLGRAGAGTVME
                     TAVTGLVTIFVPLPHGNGEQARNAGFLIEADAGRLVTDAELTADRLVAEVDGLLADPE
                     RLARMSATCRRLVPADSAAKLARLVLAAAQRRD"
     gene            6475..7896
                     /gene="murC"
                     /locus_tag="PKJ61_02615"
     CDS             6475..7896
                     /gene="murC"
                     /locus_tag="PKJ61_02615"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016456599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HOC12753.1"
                     /translation="MALIEPIDLLPVTELGAVHFIAVGGAGMSPIAAMYAQLGVPVSG
                     SDQADSPVLAQLAALGVRTFVGHDAGQLGAADTVIVSSAVRGTNPELAEARRRGLRVW
                     HRSAGLGALMLGRRGVAITGTHGKTTTSAMTAVLLAGAGADPSYVVGSPLSATGRADH
                     LGAGEAFVVEADESDGSFRQYPAEVVVITNIEADHLDNWGTAERYASGFAELVSADHV
                     RVVVTSADDPGTRDLIPLARAAGKQVITFGEAADADVRLSDLSFPASGARAMLTAADD
                     AGPLELAVPGSYNLRNAAAAYAAGRAIGCPGDRLRAAAASFTGTQRRFQPVGDVAGAR
                     IFDDYAHHPTEVAATLTAARGLVGCGRLVACFQPHLFTRTRDFAAEFGAALALADEIV
                     VLGIYPAREDPIPGVSGRLVADAAAGVAPGRVHYVETLTEAAPALVGLVRSGDLVVTL
                     GAGDVTKVGPELARRLAIGETPA"
     gene            7893..8609
                     /locus_tag="PKJ61_02620"
     CDS             7893..8609
                     /locus_tag="PKJ61_02620"
                     /inference="COORDINATES: protein motif:HMM:NF015739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HOC12754.1"
                     /translation="MTPISDATSRLELRRRDDRRRRWRRRLIAAAVVALVLVGGYIVG
                     FSPVLAAHSVEVKGAKVLTKAEVLDAAQVATGTPLMWVDPAGVAAKVAGLPPVAEVTV
                     LRDWPNAVRIEVVERKARLAIPAGGGYLLADATGVVFQAVEHAPSGLVVVAADPSDQQ
                     VLVDVGTVFSALSPATAAKVSRLEATSTDGISLRLLDGSRVVWGSAEQSPLKSEVLDA
                     LLPLGGTVFNVSAPGFPTRR"
     gene            8808..9863
                     /gene="ftsZ"
                     /locus_tag="PKJ61_02625"
                     /pseudo
     CDS             8808..9863
                     /gene="ftsZ"
                     /locus_tag="PKJ61_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002513921.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
     gene            9967..10704
                     /gene="pgeF"
                     /locus_tag="PKJ61_02630"
     CDS             9967..10704
                     /gene="pgeF"
                     /locus_tag="PKJ61_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002548688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HOC12755.1"
                     /translation="MYSFRNDPGADGVGIVFFDAISAAGGRLDLTLHRPETAAEPSEA
                     WRNPDWDLVEDALAVPALNAYQVHGRAVLTVTRDTDPQALATTAADALATTERGLGLA
                     VRAADCVPVLFADSSAGVVAAAHAGRVGLADGVLVATLERMAELGSQEVTAWIGPHIC
                     GECYEVPAELRDSFTAAHPAAWATTSWGTPGLDLGAAAQAQLTELGARVVRVDPCTRS
                     TPSLHSYRRDGAASGRQAGLVWLSDVP"
     gene            10784..11257
                     /gene="sepF"
                     /locus_tag="PKJ61_02635"
     CDS             10784..11257
                     /gene="sepF"
                     /locus_tag="PKJ61_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002548607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HOC12756.1"
                     /translation="MADRFKKAAVWLGLVSDDDYAEPEPGADEVTEQVVDRRDAEPSR
                     SPATVTELETRRPVKDHPRVADINRIITVHPRTYNEARTIGENFRDGIPVIMNLTDME
                     DTDAKRLVDFSAGLIFGLRGSIERITSKVFLLSPHNVTVAAEDKERLAGGFFNQS"
     gene            11294..11575
                     /locus_tag="PKJ61_02640"
     CDS             11294..11575
                     /locus_tag="PKJ61_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HOC12757.1"
                     /translation="MALVALLLGWAVWAYLVILTVRLVLGWLPVLIRGWEPRGWLSVV
                     AEFVYTVTDPPLRALGRVLKPLRVGDLQIDLAFLVLYFGLLVVQRLLAG"
     gene            11711..12826
                     /locus_tag="PKJ61_02645"
     CDS             11711..12826
                     /locus_tag="PKJ61_02645"
                     /inference="COORDINATES: protein motif:HMM:TIGR03544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HOC12758.1"
                     /translation="MTLTLEQVRQTRFHLARRNGYEPVDVDNFVDKVEATLSQLNEEN
                     ATLKQQVDALSSSEPSSIFVPGDSAEADQLKAELQGRSDELASVRGELDRLRNELSGK
                     SAELDQVRAELVAVRSDLDAARNGDVGRSDELAHIRGELDAARAEVDGLRAELDGVRG
                     EADQLRQVAADAQAAAVAAQGAPVEPVAPTGKVDNIVVTTSAEASPAVTRLLQMATEQ
                     AERLVGESQVEAQRIVASARAEAESVIDGANKKAHETLTDARTRSDRIESEARVNAEK
                     LVADARNNADSVNGDADRRRAELFSELERERDVLRGKVDHLRSFESNFRASLTNHLQS
                     QVKVLNDAVIEPGDVPEILSEPAPESATPRLDALLGE"
     gene            12936..13376
                     /locus_tag="PKJ61_02650"
     CDS             12936..13376
                     /locus_tag="PKJ61_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HOC12759.1"
                     /translation="MAARANGIEPASPATGVHFPVAEGEDPWTEEEVEEIRQELLADI
                     ARMERAIEVAEAGLADLFEEGSDGAGRDPADVGSSNFERDQEMSLAQNARDMLEQAQL
                     AYKLFESGDYGSCESCGQPIGKDRLQVFPRATMCVTCKQREERR"
     gene            13421..13909
                     /locus_tag="PKJ61_02655"
     CDS             13421..13909
                     /locus_tag="PKJ61_02655"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002513915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HOC12760.1"
                     /translation="MLGLGAAGLALDQVAKTVAIARLDPQQPIALLGGLVTLQLIRNP
                     GAAFSMGENFTVVLTGVAICALIGVLGWLVPRVRHTGWAVAVGFLLAGITGNLTDRLL
                     REPGPFRGHVVDFIQLPNFAIFNVADMFITAAAVLVIWLSMITQVTPAGVVTKPQPQA
                     RG"
     gene            13902..14816
                     /locus_tag="PKJ61_02660"
     CDS             13902..14816
                     /locus_tag="PKJ61_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002530528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOC12761.1"
                     /translation="MASVWLVPDGLDGDRVDAAASRMTGLSRSRIEDLAAAGGVLVNS
                     VPVAKSHRVRAGDLLEVEITTAPTVSVVPVEVAGISIVYDDADIVVVDKPAGVAAHPS
                     LGWDGPSVVEHLAAAGFRISTSGAAERQGIVQRLDVGTSGLMVVAKSEPAYTALKRAF
                     KARTVEKTYHTLVQGLPDPLAGTIEAPIGRHPGADYKMAVVSGGRHSVTHYQMLEAFR
                     AVTLLEVHLETGRTHQIRVHMQAIRHPCVGDPTYGGDPVLAAQLGLDRQWLHAVELGF
                     VHPRSGDPVRFTSPYPDDLARALALVGG"
     gene            14856..16262
                     /gene="pyk"
                     /locus_tag="PKJ61_02665"
     CDS             14856..16262
                     /gene="pyk"
                     /locus_tag="PKJ61_02665"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HOC12762.1"
                     /translation="MRRAKIVCTIGPATDSAEAIEELVTAGMNVARLNMSHGDHLLHA
                     KRVGWIRDAARSANRPIGILADLQGPKIRLGKFPDGPVMLVNGSRFTITTEDVPGDAQ
                     RASTTLSTLTDDVTAGDQILINDGAIELKVLEVTDADVVTEVVVGGMVSDHKGINLPG
                     VPVSVPALSEKDERDLRWALRHGVDMVALSFVRNAADIESVHRVMEEEGRRVPVIAKL
                     EKPQAIDNLDAIIDTFDAFMVARGDLGVELPLEDVPAVQKRIIDAARIWAKPVIVATQ
                     MLESMITSPRPTRAEASDVANAILDGADAVMLSGETAVGEFAIEAVSVMARIITKTEA
                     DGLDKIRHLRWDPHTVSGVISKAAVEVAERTEAKYLAAFTISGDTPHRLARLRSAVPL
                     MAFTPLPKTAQELTLCWGVQSYVTPEYTNTDAMIDSVQMGLLETGFVSKGDRVVIVAG
                     NPGRHTRQTNSLRVYEID"
     gene            16602..17717
                     /locus_tag="PKJ61_02670"
     CDS             16602..17717
                     /locus_tag="PKJ61_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016665988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HOC12763.1"
                     /translation="MRAAVLVAPGKPLEVRWIRTPQPRAGEVLIKVTACGLCHSDLHV
                     MAGKIPFPTPAVLGHEVSGTIVALGEELHHTDLKVGDAVCGAFLMPCGQCPDCARGRD
                     DLCTPFFRFNRLQGQLFDGTSRLADLDGNVIAQYSMGGLAEYCVIPVTAVTPMVPGMD
                     AVAAAILGCAAMTAYGAVRRGADLRYGETVAVIGIGGVGSNIVQVAHELGARKVIAID
                     VDNEKLEAAIRYGASDVINSRHLDARERVFDLTGGRGVDVAIEALGIPATFEQALTLL
                     ADGGRMVPIGLAAGAQTAAVEINRLVRRGQQICGSYGARTRTDLPIVVDLAARGVLDY
                     RGVVSERVSLFNAEATYRRLEEGHVSGRAVVDMALNG"
     assembly_gap    18178..18277
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(18279..18361)
                     /locus_tag="PKJ61_02675"
     tRNA            complement(18279..18361)
                     /locus_tag="PKJ61_02675"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18326..18328),aa:Leu,seq:caa)
     gene            18466..19098
                     /locus_tag="PKJ61_02680"
     CDS             18466..19098
                     /locus_tag="PKJ61_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002513909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOC12764.1"
                     /translation="MANSDENDRPTNELRVLVAEDEALIRLDLVELLTDEGYSVIAEA
                     GDGEEAVELARKHQPDLVVMDVKMPKMDGISAAAIIAEERIAPVVMLTAFSQRELVER
                     ARDAGAMAYVVKPFDASDVVPAIEIAMARFAEIRAVEAEVADLEERFESRKAVDQAKG
                     LLQEGLGLSEPEAFRWIQKTAMDLRKSMREVAEGVIDHSKAGGKPKPLKK"
     gene            complement(19103..19513)
                     /locus_tag="PKJ61_02685"
     CDS             complement(19103..19513)
                     /locus_tag="PKJ61_02685"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HOC12765.1"
                     /translation="MIPDWFAGLRSPLDDKMGVELLEVTPTRAVGRCPVEGNTQPFGL
                     WHGGASCVLAEGLASMAAAAEVGPEGAVTGVDINATHLRSARSGWITGTATAIRIGGT
                     LATYDISLVDDEGEQICAARITVFLRRAKPDAAE"
     gene            19637..22324
                     /gene="polA"
                     /locus_tag="PKJ61_02690"
     CDS             19637..22324
                     /gene="polA"
                     /locus_tag="PKJ61_02690"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017933813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HOC12766.1"
                     /translation="MTNPADRPETLLLIDGHSVAYRAFFALPVENFSTTTGQHTNAVY
                     GFTSMLINVLRDEQPTHLAVAFDVSRKTFRSEQYAEYKANRSASPAEFTGQVGLIKEV
                     LDALNIVHLERDGFEADDIIATLARQGAAAGLRVLICTGDRDTLQLVNDRVTVLYPRK
                     GVSDLARLDPATVLEKYLVTPDRYPELAALVGESSDNLPGVPGVGPKTAAKWLASYDG
                     LENLVRHADEIGGKVGDSLRAHLDDVVRNRSLNALVGDLELPVAVPELQRRDWDREAV
                     HSLFDGLEFRVLRDRLLETLPAADEETQGGFEVSGETLASGTVRTWLDTHARTGVVGL
                     DVTGRWRAGAGDITGLALAAADGAAAWIDVTELSPDDDRALADWLADEAAPKAMHDAK
                     GPMLAIWSRGWELAGLASDTQLAAYLLRPDQRVYDLADLVARYLKRDLAVAGGGSAES
                     DQLAFSFDDTDSHDAMVRARAVIDLAAALEAELGAQGGASLLAEVELPLTRTLAKMER
                     VGVAVDLDHLNRLHTDFDAAVAAAEADAFAVLGHPINLGSPKQLQAVLFDELGMPKTR
                     RTQTGYTTDAESLESLYAKTEHPFLAHLLRHRDQIRLRQTVEGLLKSVADDGRIHTTY
                     VQTIAATGRLSSTDPNLQNIPIRTEAGRRIREAFVVGAGYETLLTADYSQIEMRIMAE
                     ESADAGLIEAFASGQDFHTIMASRVFGVAAGEVTPAQRAKIKAMNYGLAYGLSAFGLS
                     QQLKIERSEAAGLMEEYFERFGHVRDYLREIVATASRTGFTETILGRRRYLPDLTSSN
                     RQRREMAERAALNAPIQGSAADIIKVAMLDVETAIERRGLRSRMLLQVHDELIFEVAP
                     GEREDFEELVRDKMGHAVEMRVPLEVSVGYGHSWHAAAH"
     gene            22327..22938
                     /locus_tag="PKJ61_02695"
     CDS             22327..22938
                     /locus_tag="PKJ61_02695"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOC12767.1"
                     /translation="MSEQPAQRELWQRSAARYDQATAWLERRMLARERERLIPQARGR
                     TLEVGVGTGANLPWYPHEVELFGVDASPAMLDLARKRAARLGRQAWFELGDADALSHP
                     EASVDTVVATLLLCSVPKVESTLAEFARVLRPGGRLLLLDHVESSSAFVRAGQRLADL
                     VTSRTGERWRRRPLRQLPDAGFDVVQTHITRARLLEVVVAERR"
     gene            complement(23078..23647)
                     /locus_tag="PKJ61_02700"
     CDS             complement(23078..23647)
                     /locus_tag="PKJ61_02700"
                     /inference="COORDINATES: protein motif:HMM:NF037041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein"
                     /protein_id="HOC12768.1"
                     /translation="MSGTAKIEDATLTPSKLELLAGWLPSQSWFKGSPDDLTRVASYR
                     FVDPDGEVGIETILVRSAGVTYQVPLTYRSAPLDEAADALVGTMEHSVLGTRYTYDAV
                     ADPVYVVELLRVIHEGDTEADLSRGEKSMTVEGSGIVLVSNAAGESARLVRVLDGSHV
                     PGTRQPLGTLTGKWAEESGDQEQVLAVIR"
     gene            23879..25336
                     /gene="rpsA"
                     /locus_tag="PKJ61_02705"
     CDS             23879..25336
                     /gene="rpsA"
                     /locus_tag="PKJ61_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016456583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HOC12769.1"
                     /translation="MTSSSEAATVAVDDFGSPEAFLAAVDATIKYFNDGDIVTGTVVK
                     VDRDEVLLDIGYKTEGVIPSKELSIKHDVDPFDVVSVGDVIEALVQTKEDKEGRLILS
                     KKRAQYERAWGTIEKVKEEDGVVTGRVIEVVKGGLIIDIGLRGFLPASLVEMRRVRDL
                     QPYVGAELEAKIIELDKNRNNVVLSRRAWLEQTQSEVRHTFLNQLTKGQIRKGVVSSI
                     VNFGAFVDLGGVDGLVHVSELSWKHIDHPSEVVEVGDEVTVEVLSVEMDRERVSLSLK
                     ATQEDPWQTFARTHQIGQIVPGKVTKLVPFGAFVRVEEGIEGLVHVSELAERHVEIPE
                     QVVTVNDEVLVKIIDIDLERRRISLSLKQANEGVDIAADDFDPALYGMSASYDEQGNY
                     IYPEGFDPETQEWKPGYEAQQRAWEQQYAEAQARWEAHKKQVEEAEAADVQAAVTEAN
                     TAAYSAGAADTSAPEGSLASDEALQALREKLTGGR"
     gene            25416..26093
                     /locus_tag="PKJ61_02710"
     CDS             25416..26093
                     /locus_tag="PKJ61_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC12770.1"
                     /translation="MYPQGAAVTDNRFENYPPAPTFAAHDDVAAEPVSGVVTPAKPYS
                     IPPRTRRPSRRVMLALMIGVPVAVGLGIALNVDDDGDPEVDTGSFLLADHEISVPLNW
                     TYELSADGEAATLTRAGNTVKIAAVRRFDGEQAINVIGEQTGALVPDYTSRRMQEPVD
                     SSSGRFDRATLTGTAELHGVHVRILSNLWLTEGEGLIAVRLLTEASRSSYTTEAAEIV
                     RAVGENF"
     gene            26093..26839
                     /locus_tag="PKJ61_02715"
     CDS             26093..26839
                     /locus_tag="PKJ61_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC12771.1"
                     /translation="MSDQNPTQFENYPTEPGDMGAVTEPGPGASSAPLVVPAPPAQIA
                     DWSADTEKSDGTARRVVIGSIVGIVALGFIGSVSRNSGDGESSEVSFTAAAPEETIYE
                     YEEEPESGTPLELGGYAATLPDGWTLDNTSDQLAVATCGTNRVLVRLLDNSGLSATER
                     ITGAMKGRLGSFKGSLDEPEDVSFGSTDAARIVGTGKIGKTAARLIGQLWVDADDNSL
                     LVVQTLTAKPDSERALQAQDIADQLSSNFG"
     gene            26836..27492
                     /gene="coaE"
                     /locus_tag="PKJ61_02720"
     CDS             26836..27492
                     /gene="coaE"
                     /locus_tag="PKJ61_02720"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002548796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOC12772.1"
                     /translation="MSGSTVLVGLTGGIASGKSTVADLLAAHGAVVIDADLLAREVVE
                     PGSPGLVAVVERFGTGVLRADGSLDRPALGRLVFADTDARRDLERIIHPAVRERAAEL
                     VASAEPGSVVVQMIPLLVETGQQDAFDLVVVVDVDPGVQLARVRVRDGLSEAEALARI
                     GAQASREARLAAADVVITNNGGLGELREQVDKLWREFTRNEPPAAPGWRAKRNVRGDA
                     "
     gene            27503..29584
                     /gene="uvrB"
                     /locus_tag="PKJ61_02725"
     CDS             27503..29584
                     /gene="uvrB"
                     /locus_tag="PKJ61_02725"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HOC12773.1"
                     /translation="MRPITDVQRRVAPFKVVADFEPAGDQPAAIEELERRLTAGEQDV
                     VLLGATGTGKTATIAWLAERVQRPMLVMQPNKTLAAQFANELRQFFPDNAVEYFVSYY
                     DYYQPEAYVPQTDTYIEKDSSLNEEVERLRHSATNSLLTRRDVIVVATVSAIYGLGTP
                     QEYVDRMVRLRVGEETDRDGLLRRLVDIQYVRNDLAATRGTFRVRGDTVEVFPMYEEH
                     AVRVEFFGDEIEALSTLHPLTGEILSNDQEIYVFPASHYVAGPQRMERAIDGIESELA
                     VRLADLEANSQLLEAQRLRMRTGYDIEMMRQIGTCSGIENYSRHIDGRAQGSAPNCLL
                     DYFPEDFLLVVDESHVTIPQIGGMYEGDMSRKRTLVEHGFRLPSAMDNRPLRWEEFVD
                     RIGQTVYLSATPGPYEMAKSDGYVEQLIRPTGLVDPEIVLKPTKGQIDDLMAEIRARA
                     ERNERVLVTTLTKKMAEDLTDYLMENGVRTRYLHSEVDTLRRIELLRELRLGVYDVLV
                     GINLLREGLDLPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVAGQVHMYADKITP
                     SMAAAIDETNRRREKQVAYNTERGIDPQPLRKKIADLTDLLAREDADTEGLVSRAGGR
                     RPVSPTPMSTQKNRDLGAMPSSELAGLISELNEQMHSAAAELQFELAARLRDEIADLK
                     KTLRQMLEATK"
     gene            29643..30617
                     /locus_tag="PKJ61_02730"
     CDS             29643..30617
                     /locus_tag="PKJ61_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004811766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HOC12774.1"
                     /translation="MHVTLEVWLVTIAIMAAVLIVDLVVIGRRPHEPSMREAGTAIGI
                     FSGLAVLFGLGVWWFAGARFAGEFFAGWLTEYSLSIDNLFIFVIIMANLRVPRQLQQF
                     ALMVGIVLALVFRGIFIALGAAAIERFSWVFFLFGAFLIWTAAKLYIDYRQHDEEEEA
                     TENAVMRWVKRTLPSTPDFHGTKLTVVAAGKRLITPMFFVIVALGTTDLLFALDSIPA
                     IYGLTQEPYLVFAANVFALMGLRQLYFLIGGLLQRLVYLAVGLSVVLAFIGVKLVLHA
                     MHEYHWDERLGFNGEIPIWASLTFILVTLGVTTVASLVKSRRSDALPE"
     gene            complement(30606..31313)
                     /locus_tag="PKJ61_02735"
     CDS             complement(30606..31313)
                     /locus_tag="PKJ61_02735"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HOC12775.1"
                     /translation="MKWHIGTVPTDDVHRWQAEATQRLLGYTIVLDDDSWHAPTTLPG
                     WSRAHVATHLARNADHVRGVLEAVSAGVPQPEPLVRSDRIAALEVGADRDGLALQIDL
                     DTSAGALHRITDGTTDWDHEVDIHGRRHPLSALALARLHEVCIHHLDLDFDFAPELID
                     PAAAAWLLRWVIDCLQDADLPALRIEADSLVTELGHGADRLVVSGSDVRLWAWLSGRG
                     TPDWVTGADGFQPTLLG"
     gene            31393..34284
                     /gene="uvrA"
                     /locus_tag="PKJ61_02740"
     CDS             31393..34284
                     /gene="uvrA"
                     /locus_tag="PKJ61_02740"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016667351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOC12776.1"
                     /translation="MFDVTDRLIVRGAREHNLRNINLDLPRDAMIVFTGLSGSGKSSL
                     AFDTIFAEGQRRYVESLSAYARQFLGQMDKPDVDFIEGLSPAVSIDQKSTSRNPRSTV
                     GTITEIYDYLRLLYARVGRPHCPECGEPISRQSPQQIVDRLMGLPEGTRFQVLAPVVR
                     GRKGEYVDLFAQLAGQGLSRVRVDGEVHQLLQPPTLAKQKKHNIDVIVDRLAAKPSIK
                     QRLTDSVETALGLAGGVVAIDFVDLDDHDPKRERRYSERLACPNEHDISVEELEPRQF
                     SFNGPWGACPACSGLGTRMEVDVDLVVPDPSKTLAQGAITVWTTPYVAGYFQSLFKAL
                     GAQEGFSVDTPWEELPSRAQSLLLMGAPHAITVHATSRHGREHSYRAKYEGALPYIRR
                     RHAEAESENSRERFAGYMREVPCLTCNGARLKPSSLAVTIDGRNIAEVSAMSIDEVAG
                     FMRGLKLPEREAQIAERVVKEVNERLRFLLDVGLDYLSLARPAGSLSGGEAQRIRLAT
                     QIGSGLVGVLYVLDEPSIGLHQRDNRRLIDTLVRLRDLGNTLIVVEHDEDTIRTADWA
                     VDIGPGAGEHGGEVLVSGPVAELLAHPTSLTGAYLAGRRRIPMPSRRRPATGKAITVH
                     GAAEHNLRNVTVEFPLGQLIAVTGVSGSGKSSLVNSILYTALAKKLYNAHTVPGRHRT
                     ITGIEHIDKSIHVDQSPIGRTPRSNPATYTGVFDHIRRLFAETPEAKVRGYQPGRFSF
                     NVKGGRCENCAGDGTIKIEMNFLPDVYVPCEVCHGARYNRETLEVHYKGRTIAEVLDM
                     PIEEGVDFFGAIPAIARHLRTLVDVGLGYVRLGQPATTLSGGEAQRVKLAAELQKRST
                     GRTIYVLDEPTTGLHFEDIRKLLQVLQSLVDKGNTVVVIEHNLDVIKAADWVIDLGPE
                     GGSRGGMVVAEGTPEQVADNPASFTGAFLKAVLDGQRVPVGESEPALLA"
     gene            complement(34292..34714)
                     /locus_tag="PKJ61_02745"
     CDS             complement(34292..34714)
                     /locus_tag="PKJ61_02745"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HOC12777.1"
                     /translation="MTNPLPSRRAALITIGGTTLAVCLSGCAPSSSGTEAAGGGASGG
                     AAADGNTVSAADVPVGGGKIVGQYVVTQATEGDFQAFSYLCTHQNLPVQDVSDAAIIC
                     GRHGSTFSLADGSVLTGPATKGLTKATVTVNGNSLSIS"
     gene            34944..36890
                     /gene="uvrC"
                     /locus_tag="PKJ61_02750"
     CDS             34944..36890
                     /gene="uvrC"
                     /locus_tag="PKJ61_02750"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOC12778.1"
                     /translation="MADPASYRPAPGTIPTGPGVYRFSDREGRVIYVGKAINLRQRLN
                     SYFADPSALHFRTQTMVRTAAGVEWTLVRNELEALQLEYTWIKQFDPRFNVKYRDDKS
                     YPWAAVTWSEEFPRVFVGRGSKRKGVRYFGPYAQAWAIRDTIDSLLRVFPMRSCTNGV
                     FRSARASGRPCLMGYIGKCAAPCVDRIDPDAHRVLVEDVCDFLAGRTAALTRRLEAEM
                     RIAATNLEYERAAVIRDQLVALKQASERNAIVLGDGTDADVIALAEDPLEVAVQVFNV
                     RDGRVRGERGWVAERSDDADTGRLLEQFLLQLYAEDAESIPPEILLPALPTSPEVLAQ
                     LLGDERGTKVKLKVPKRGDRAALLETAARNATDALTLHKVRRASDLSTRNRALEELQE
                     ALGLPEVPLRIECYDISHTQGSEVVGSMVVFEDGLARKSEYRRFIIRTVEGSNDVGAM
                     REVITRRFNRLLAENERTGEESGPLLVDPTTGAPRKFAYLPGLVVVDGGAPQVAAAQA
                     ALSELGIQNVALCGLAKKLEEVWLPGEEYPLILPRASEGLYLLQRVRDEAHRFAITHH
                     RSRRSASMVESALDDVPGLGEIRRKALLKYFGSLRKLRAATVEQLAEVPGVGLKTAAA
                     VKEALAQHGPAEAINLTTGEIIQT"
     gene            36925..37791
                     /gene="rapZ"
                     /locus_tag="PKJ61_02755"
     CDS             36925..37791
                     /gene="rapZ"
                     /locus_tag="PKJ61_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016455754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HOC12779.1"
                     /translation="MQSTPAVRLLVLTGLSGSGKRTAGHTLEDLGWLVVDNLPPSLLP
                     DLIEVSRQREFNRVAVLLDVRSRSEFQQIPEAFATLGEAGIVPQIVFIDASDETIVRR
                     QESVRRPLPLQGPGRILDGIRRERELLAGLRASADLVIDTSALNVHDLAARVTVAFAD
                     EDEAQLKLTLLSFGFKNGIPIDADIVLDVRFLPNPHWVPALRPQSGLSQPVAHYVLGQ
                     PDAEPFLDHVLGMIRLMIPGYVREGKRHAMVAIGCTGGKHRSTAIVEEIARRLAVDGT
                     PAEVVHRDLGRE"
     gene            37788..38738
                     /gene="yvcK"
                     /locus_tag="PKJ61_02760"
     CDS             37788..38738
                     /gene="yvcK"
                     /locus_tag="PKJ61_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HOC12780.1"
                     /translation="MNFTKPPAVVAFGGGHGLAASLSALRQLTHRLTAVVTVADDGGS
                     SGRLRDEFPILPPGDLRMALAALCGDDEVGRAWAEVLQSRFPGEGPLGGHSIGNLLIA
                     GLWQQRADPVAGLDMVASMLHVEGRVLPMAAVPLVIEADVIGADPEYPDDTMVVVGQE
                     EVALANGDIGDVRLVPANPPAVPEALRAIAEADHLVFGPGSWYSSVIPHVLVPELLDA
                     ILASPAQRILTLNLVPTADETPAYTASRHVEVLAEHAPGLRLDAVVADPSFADGDTHL
                     ATYVRSLGARLVVAPVRMRDGSPRHDPHLLSAVYAGIMGL"
     gene            38788..39774
                     /gene="whiA"
                     /locus_tag="PKJ61_02765"
     CDS             38788..39774
                     /gene="whiA"
                     /locus_tag="PKJ61_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HOC12781.1"
                     /translation="MAMTQQVKAELATLEVTKPELRRAEVAALLRFANGLHIAGGRIV
                     VEAELDTGAAARRLRTAIGDLFGFNCEFVVIAGSGLRRGARYLVRLIRDGEALARQTG
                     LIDSRGRPVRGLPVQVVSGGLDVSVAAWRGAFLAHGSLTEPGRSMALEVTCPGSEAAL
                     ALVGAARRLGISAKAREVRGIDRVVIRDGDAIAAMLTRLGAHESVLAWEERRMRREVR
                     ASANRLANFDDANLRRSARAAVAAGARVKRALEILGDDIPEHLLEAGRLRLDNKQASL
                     EELGQLHVPPLTKDAVAGRIRRLLATADKRAAELGIPNTEVGLSPEMLDDDG"
BASE COUNT         6287 a        13291 c        13800 g         6312 t
ORIGIN      
        1 tgggtaaggg ccatgagacc ggccagcagt tggctggccg aacgatcgag ttcgacgacg
       61 cccggacggt gctccggctg tgggaagagt tgaggggagc cgtcgatgcg tgagcgcaac
      121 tcacgggagt tggctgggtt gctcggggcc gagttgaccg gggacggcga cgtgccgatc
      181 ggtccggatg tggtgatcga ctccagggcg gtcaccccgg gctgcctttt cgtggcgatg
      241 ccaggagttc gggtcgacgg acacgatttc gccgccgcag ctgtcgaagc gggcgctgcc
      301 gcggtgttgg tgagccggga ggttcccgtc ggcgtcccgc agttggtggt ggctgatccg
      361 gcgctcgccc tggcccggtt ggccaccgcg gtcgtggccg atgccaccgc ctctggcctg
      421 gtcacgatcg gcatcaccgg gtcgtcgggc aagaccagca ccaaggacct gctggcccag
      481 gtgctcgcca cggcggggga gaccgtggcc ccggtcggct cgttcaacaa cgagatcggg
      541 gtgccgctga ccgccacccg ggtcggtccg accacgcggt acctggtcag cgaaatgggc
      601 gcgcggggca tcggtcacat cggctggctg tgccgaatcg tgccgccgca ggtcggcgtg
      661 gtggtgaaca tcgggcacgc ccacctgggc gagttcggct ccgtcgagac catcgcgcag
      721 gccaagggcg aactggtcga ggcgctgccc gcttccggct gggcggtgct gaacgcggac
      781 gacgagcagg tgcggaccat ggccggacgc acgtccgcgc gactggcggc cttcggtgtc
      841 gatgccgaac ccgacttcgg tgaactgcgg gtgtgggcga gcgacgtcca agccgacctg
      901 ctggagcggg cccgcttccg tctccatgcg gccggcgccg cctttggtga ggccgtggtc
      961 gaactccagg tcaccggacg gcaccaggtc ggcaacgcgc tggcggcggc ggcggtggcc
     1021 ctcacccagg ggctgtcgct cgcacaggtc gcgaccggcc tgacccaggc tgtggcgcga
     1081 tcgcactggc ggatggagct cacggctcga cccgacggcc tactagtggt caacgacgcc
     1141 tacaacgcga accccgactc gatgcaggcg gcgctgcaga ccctcgcgtg cctgcggcag
     1201 ccggacggac ggttgctggc cgtcctcggc gacatgctcg agctcggcga cggtgccgcc
     1261 gcagcacacc gggacatcgg cgcactggcc gctcggctgg gtgtcgacca cctgatcgca
     1321 gtcggcgact tcgccgccga cctggtcgcc ggcgcgagcg cggacgggct gcacgcacag
     1381 atcgcagcgg accgagagca ggccgcagag ctggccgcgg cctggctgcg tccgccggac
     1441 gtcgtgctgg tgaaggcgtc ccgaggacta gcgttggagc acgtggccga actgttgctc
     1501 gcggatctga ccggtacgcc cggggaaggg tgaggcgtcg atgaggagca tcctgctcgc
     1561 cggcggggtc ggcatgctgg gcaccctgct gggcacccgc tacgcgatcc gcttcctggt
     1621 gaaggccggc tacggccagt tcatccgcga tgacgggccg accagccacc acaccaagcg
     1681 cggcaccccg accatgggcg ggatcgtgct catcctgagc gtcctgctgg cttacgccgt
     1741 cgcgcatctc gccaccggca ccccgcccag cgcaaccagc ctgctggcgc tcggcctgtt
     1801 cgccggcctc gggctggtgg gcttcatcga cgactggatc aagatcagca aggcccgatc
     1861 cctcggcctg cacgcatcgg tcaagttcgg cctgcagact gccgtcgcgg tcacttttgg
     1921 catcctggcc ttccagttcc cggacgcccg cgggctcacc ccggcaagca cctacatctc
     1981 cttcctgcgc gacctgccct ggttcgccct gccgatgtgg gcagcggtga tctggatcgt
     2041 cttcctggtc gcggcctggt cgaacgccgc caacctcacc gatgggctgg atggcctggc
     2101 caccggcgcc gcggcgatgg tcttcgcggc gtacaccatc gtgaacatct ggcagtacaa
     2161 ccagtcgtgc gccctggcca gttccgggcc gcagtgctac gaggtgcgca acccctacga
     2221 cctggcggtg gtctcgatcg cgctcgccgg tgcctgcttc gggttcctgt ggtggaacgc
     2281 ttcgccggcc aagatcttca tgggcgacac cggctcgctg gcgatcggcg gcgcgctggc
     2341 cagcctctcg atcctcaccc gcaccgagtt gctgatggcg atcgtcgccg gcctgttcgt
     2401 gatcatcacc gcatcggtgg tgctgcagat cggctggttc aagatctccg gcggcaaacg
     2461 cctgttcaag atggctccgc tgcagcacca cttcgagttg cttggctgga aggaagtcac
     2521 gatcaccatc cggttctgga tcatctgcgg actgttcgtc gcggtcgggc tcggcctctt
     2581 ctacggcgag tgggtggtgg ggtgaagccc tggctggccg gcgccgaccg gttgtcgccg
     2641 tggtcgcagg ccaaggtcgt cgtggccgga ctcggcacgt ccggcttcgc cgctgcggac
     2701 gggctgctcg acctcggcgc ccaggtgctg gtgctggacg actccgactc ggccgacaac
     2761 gccgagaagg ccaaggtcct cgaactgctg gacgcggagg tgcgcctcgg cccgggcgct
     2821 accgccgtcc tcccggacga caccgacctg gtggtcactt cacccggctg gcgtccgtcc
     2881 gcgccgctgc tggcacaggc tgcgctgcgc ggcgtcccga tctggggtga ggtggaactc
     2941 gcttggcgga tgagcatgcc cgaccggcag gtgccgtggc tggcggtgac gggaaccaac
     3001 ggcaagacca ccaccgtcgg catgctggag tctatgctga cggcagcagg gctgcggacg
     3061 tccgcggtcg gcaatgttgg gcgtccgatc gttgaagccg tgctggatga ggtcagttac
     3121 gacgtgctgg cggttgaact ctccagcttc cagttgcatt ggacgaacag cctctcgctg
     3181 cactcggcgg cagtgctcaa cctcgcgccc gaccacctgg agtggtacgc cgatcgcgcc
     3241 ggctggccgg acggcgtgac cgacccgatg gcggcctacg tcgccgacaa ggcccggatc
     3301 tacgaacgcg tgcagcagtc ctgcgtctac aacgtcgctg atgacgccac catgcacctg
     3361 gtcgaggcgg ccgaggtggt cgagggcgcc cgggcgatcg gcttcaccct cggcatcccg
     3421 gggccgtcca tgctcggggt ggtggacgat gtactggtcg accgggcctt catcgcccaa
     3481 cgcagggact cggccgtcga gatcgccaag ctgagcgacg tggcgccgta cgccccgcac
     3541 aatgtggaga acgcgctggc cgcagccgcg ctggcccgat ccttcggggt gccgccgacg
     3601 gccgtggcgc agggcctgca gcggctgcgg ctcggcgacc accgcatcca gactgtcgcc
     3661 gagatcggcg gcgtccgcta catcgacgac tcgaaggcca ccaatccgca cgcggccgcg
     3721 gcatcgctgg gcgcattcga gcatgtggtc tgggtggccg gcggccaggc gaagggcacc
     3781 accttcgacg acctggtcac cgcggttgcg cccagacttc gcgcagcggt ggttttcggg
     3841 gtggatcgtg acgtgatcgc cgccgcgctc gcccgacacg cgcccgaggt gcgggtgttc
     3901 gtgatcgagg accgcgatca tggagcgatg caacaggcgg tggagtgggc ggcgacccag
     3961 gcggtgcctg gcgacgtggt gctgctcgcc ccaggctgcg ccagcaagga catgttcagc
     4021 gactacgcag cccgcgggga cgccttcgcg gccgcggtct cgaccctgac cggataggag
     4081 gcgcgaactg tggcggtcct gacttcccct tcaacggatc ctcacctatc gaccaatcgt
     4141 cgcggccacc aggcggcccc ggccgacggt cgtcggcgca gtctcgtcgt cgagtggctc
     4201 gcgcatccgc aggcgagctt cttcctggtg ttggtgcccg cggtgctgct gctggcgctc
     4261 ggcatgatca tggtgctctc ggcgtcgagc gtctacgcct tcgtccgctg gggcgacgcg
     4321 tactacttcg tgaaacggca ggccatcttc ctggtggtcg gtggcgtggc cgcgttcgtg
     4381 ctggcggcgc gctccactaa gcagctgaag gttctcggct ggattctgtt gatcgccgcg
     4441 gtcgggctgc agatcctcac ttacaccccg ctcggctact cgaacaacgg caacaccaac
     4501 tgggtgcagt tcggcacctc gctgttccgc attcagcctt cggagttcgc caagctggcc
     4561 atcgtgctgt ggggggcgga catcttcgcg cgcaagcgaa agcaactcgg cgacccgcgg
     4621 cacctgttga tcccgttcct gccggcctcg ttgatcctga tcggcctggt cgtgctgcag
     4681 tccgacctgg gcacaggcat gatcatgggc gcactggtgc tggcggtgct ctggtacgtc
     4741 ggcgcgtcct ggaaagtgct gggcgcgatc gtgggctccg ttgctgccgc cgtcgggttg
     4801 ctggtggtca cctcgcccta ccggatgaac cggatccttg gcttcttcaa ccaggaagtc
     4861 gaccagttgg gcgtcaacca ccagccgatc aaggcgatct tcgcgctcgc gtccggcggt
     4921 tggtgggggc tggggctcgg tgccagccgg cagaagtggg gtggcctggt ggagtcccac
     4981 accgactacg tgctggccgt gatcggcgag gaactcggcc tgatcggcac gctgagcgtg
     5041 ctgatcctct tcctgatcct cggctacgcc ggcttccgga tcgcgatgcg ctccgacatc
     5101 cggttctgcc gctacgccgc agccggtgtg accagctggt tcatgatcca ggggttggtc
     5161 aacatcgccg tcgtgctgcg gatgatcccg gttatcggtg tgcccctgcc gctgctctcc
     5221 tacggcggct cggcgctgat ggccaatctg atggcgctcg gcgtgctgct ggcctgtgcc
     5281 cgccaggagc cggagtatcg cgcgttcgcc cgccgccggc gcagccgcaa ggaacgggca
     5341 ctggtgacta ccgccgcggg acgcgcgtga acgccgtccg aatcgttctc gccgggggtg
     5401 gcaccgccgg acatacctcg ccgctgatcg ccaccgcgaa ctcgctggcc gaactggccg
     5461 aggtcgacgt cgtctgcgtg ggcacgccca agggcctgga gtccagggtg atcccggacg
     5521 cgggcctgcg gctgcagttg atcccgccgg tgccgctgcc gcgccggatc accccggaac
     5581 tgttcgccgt gcccggacgc ctgtggcggg ccgtccgcga agccaaggcc atcctggtcg
     5641 cccacggggc ggaggtggcg gtcggcttcg gcggctatgt gtcgttgccg gtctatctcg
     5701 cggcgcgtca ggccaaggtg ccgctggtgc tgcacgaggg caactacgtg cccggtctgg
     5761 ccaaccggat cgctgcccgg ttcaccccgc atgtggtgac cactttcgcc gagaccgccc
     5821 tgccgcacgc ccagcacctg gggctgccgg tgcgcgaggc catcgccacc ctcgaccggg
     5881 acgcgcggcg tccggctgct cgggaagcgt tcggattgcc cgcggacggg cgggtgctgc
     5941 tggtcagcgg gggatcgcag ggcgcccgca gcctcaacca ggcgacgctc gccgcgcgcg
     6001 agcggctgct ggccgccggg atctcggtgc tgcacgtgct gggcgcggcg aacctcactg
     6061 ccgaggatgt accggtcacc cacccgaaca ccggtgcggt ctaccggccg gtgggctacg
     6121 ttgacgagat ggccaatgcc tacgccgcgg ccgatctgat gctgggccgg gccggtgcag
     6181 gcacggtgat ggagaccgcc gtgaccggtc tggtcacgat cttcgtcccg ctgccgcacg
     6241 gcaacggtga gcaggcgcgc aacgccggct tcctgatcga ggcggacgcg ggccggctgg
     6301 ttaccgacgc cgagctgacc gccgaccggc tggtggccga agtggacggg ctgctggccg
     6361 atcccgagcg cctggcgagg atgtccgcga cctgtcgtcg gctggtgccg gcggactccg
     6421 cagcgaagct ggcccggttg gtgctcgcgg cggcccagcg tagagactga acccatggca
     6481 ctgatcgaac ccatcgacct gctgccggtc accgagcttg gcgcggtcca cttcatcgcg
     6541 gtcggcggcg ccggcatgag cccgatcgcc gcgatgtacg cccagctcgg cgtgccggtc
     6601 agcggcagcg accaggccga ctcgccggtg ctcgcgcagc tggccgcgct cggggtgcgc
     6661 accttcgtcg gccacgacgc cggacaactc ggtgccgccg acaccgtgat cgtgtcgtcg
     6721 gccgtccgcg ggaccaaccc cgaactggcc gaagctcgcc gccgtggcct gcgggtgtgg
     6781 catcgcagcg cagggctggg cgcgctgatg ctgggccggc gtggcgtcgc gatcacgggc
     6841 acgcacggca agaccaccac ctctgcgatg accgcggtgc tgctggccgg tgcgggtgcc
     6901 gaccccagct acgtggtggg ttcgcccttg tcagcgaccg ggcgcgcgga ccacctcggc
     6961 gccggcgagg cgttcgtggt cgaggccgac gagagcgacg ggtcgttccg gcagtacccg
     7021 gccgaagtag tggtgatcac caacatcgaa gccgatcatc tcgacaactg gggcaccgcg
     7081 gagcggtacg cgtccggttt cgccgaattg gtcagcgccg accacgtacg ggtggtcgtg
     7141 accagcgccg acgatcctgg gacgcgcgac ctgatccctc tggcacgggc ggccggcaag
     7201 caggtgatca cgttcggcga agcggcggac gccgatgtgc ggctcagcga cctcagcttc
     7261 ccggcgtccg gcgcccgggc gatgctgacc gcagcggatg acgccgggcc gctagaactg
     7321 gccgtgcccg gtagctacaa cctgcgcaat gccgcggcgg cctatgccgc cggccgggcg
     7381 atcggctgcc ccggtgaccg gctgcgggcg gctgcggcca gcttcaccgg cacgcagcga
     7441 cggttccagc cggtcggcga cgtggccggc gcccgcatct tcgacgacta cgcccaccat
     7501 ccgaccgagg tggcggcgac gctcaccgcc gcgcgcggcc tggtcggctg cggccggctg
     7561 gtggcctgct tccagccgca tctgttcacc cgcacccgcg acttcgcggc cgagttcggc
     7621 gcggccctcg cgctggcgga cgagatcgtc gtgctcggca tctacccggc cagggaggat
     7681 ccgatcccag gcgtcagcgg acgcctggtc gccgacgctg cggccggggt cgcgccgggc
     7741 cgcgtccact acgtcgagac cctaaccgaa gccgcgccgg ccctggtcgg gctggttcgc
     7801 tccggcgacc tggtggtcac cctcggcgcc ggcgatgtca ccaaggtcgg acccgagctg
     7861 gctcgccggc tggccattgg ggaaacgccg gcgtgacccc gatcagtgac gccacctcca
     7921 ggctggaact gcggcggcgc gacgaccgtc gccggcgttg gcgccggcgg ctgatcgcgg
     7981 ccgcggtcgt ggcgctggtg ctcgtcggtg gctacatcgt gggcttctcg ccggtgctcg
     8041 cggcgcactc ggtggaggtg aagggcgcga aggtgctgac caaggcggaa gtgctggacg
     8101 cggcccaggt ggccacgggc acgccgctga tgtgggtgga tcccgccggc gtggccgcga
     8161 aggtcgccgg cctgccgccg gtggccgagg tgaccgtcct gcgcgactgg ccgaatgccg
     8221 tccggatcga ggtggtcgag cgcaaggctc ggctggccat tcccgccggc ggtggctatc
     8281 tgctggcgga cgccaccggc gtcgtcttcc aggcggtgga gcacgcgccg tccgggctgg
     8341 tcgtggtggc cgcggacccg tccgatcagc aggtgctggt ggacgtgggc acggtcttct
     8401 ccgcgctcag cccggccacg gctgcaaagg tgtcccggct tgaggccacc agcaccgacg
     8461 gcatctcgct gcgcctgctg gacggctccc gggtggtctg ggggagcgcc gagcaatccc
     8521 cgctgaagag cgaggtgctg gacgcgctgc tcccgctggg cggaacggtg ttcaacgtgt
     8581 ccgcgccggg cttcccgacc cggcgttgag actggatcga gcaaccctga acccctactt
     8641 gagggacgcg ccgagcgatt cggcaatctt ggacatggca atgggtgatc cctagccttg
     8701 ggagcgcgcc gaagcctgtt tgcacttccc cgctcgcccg cttcgtcgaa ctaaatcgag
     8761 atttcacaac gtctgagcat ttcggactcg gagtaagggt gggtgtcgtg gggactgctt
     8821 cccagaacta cctcgcaatc atcaaggtcg tcggtgtcgg tggcggcggg gtcaatgccg
     8881 tcaaccgcat gatcgaagcc ggcctgcgag gggtcgagtt cattgccgtc aacacggacg
     8941 cgcaggcgct gctgatgagc gatgcggacg tgaagctcga catcggccgc gacctcaccc
     9001 ggggcctggg cgcaggcgcc gacccggcca agggccgcca ggcggccgag gaccattccg
     9061 acgagatcga agaggtgctg aagggcgccg acatggtctt cgtcaccgca ggcgaaggcg
     9121 gcggtaccgg taccggcggc gctccggtgg tggcgcggat cgcccggtcg ctgggcgcgt
     9181 tgacgatcgg cgtggtcacc cgtccgttca ccttcgaggg ccggcggcgc tcgtcccagg
     9241 ccgaggacgg catcggggcc ctgcgcgaag aggtcgacac cctgatcgtc atccccaacg
     9301 acaagctgct ggagatgacc gaccaccacg tggcgatcct ggacgcgttc cgccaggccg
     9361 accaggtgct gatgcagggc gtctccggca tcaccgacct gatcaccacg cccggcctga
     9421 tcaacctcga cttcgcggac gtgaaggcgg tcatgtcgaa cgccggctcc gccctgatgg
     9481 gcatcggctc ggcccggggt gaggaccgcg cacgcgcggc tgccgagatg gcggtctcct
     9541 cgccgctgct ggaggccagc atcgacggcg cccacggcgt cctgctctcg atcgccggtg
     9601 gctccgacct cggcctgttc gaggtctcgg ccgcggccaa cctgatcgag gccgccgcgc
     9661 acgaggacgc caacatcatc ttcggcacgg tcatcgacga cgccctcggc gacgaggtcc
     9721 gggtcaccgt gatcgctgcc ggcttcgacg gcggccagcc gcccaagaag gtcaccgcgc
     9781 ggcccgagcc gcagcgccgt ccggaagtgg ttcgtccggc cgcgccggag cccaggccgg
     9841 ccccggtcga gcccgagccc gagctggcgg ccccggcgcc ggtcgtccgg ccgttcgaac
     9901 cgccggtgat cgacgacgac gaactggacg tgcccgattt cctgaagtag gccgggccag
     9961 ccccccatgt acagcttcag aaacgatccc ggcgccgacg gcgtcgggat cgtcttcttc
    10021 gacgccatct ccgcggccgg cggccggctc gacctcaccc tgcaccgacc agagacggcg
    10081 gccgagccta gcgaggcctg gcggaatccg gactgggacc tggtcgagga cgcgctggcc
    10141 gtgcccgcgc tcaacgccta ccaggtgcac ggccgggccg tgctcaccgt gacgcgggac
    10201 accgacccgc aggcgctggc caccacggcg gccgatgccc tcgccaccac tgagcgcggc
    10261 cttgggctcg cagtgcgggc ggccgactgc gtgcccgtgc tgttcgcgga ctcgtccgcc
    10321 ggtgtggtcg cagctgcgca tgccggccgg gtcggcctgg cggacggagt gctcgtggcc
    10381 acccttgagc gaatggctga actcggttcg caggaagtga ccgcctggat cgggccgcac
    10441 atctgcggcg aatgctacga agtgcccgcc gaactgcggg acagcttcac tgccgcgcat
    10501 ccggccgcct gggctacgac cagctggggc acgccgggcc tcgaccttgg cgcagcggcg
    10561 caggctcagc tcaccgagct cggcgcgcgc gtcgtccggg tggatccctg cacgcgttcc
    10621 acaccatcac tgcactccta ccggcgcgac ggcgccgcaa gcggccgcca ggccggtctg
    10681 gtctggctct ccgacgttcc ctgagcgtgt cggcaaccgc ctgcgggatc ccaggggcca
    10741 cgcggactag agtcggcaag gactggcgag aaaggcgaat ccgatggctg accgattcaa
    10801 gaaggccgct gtatggctcg gcctggtgtc cgacgacgat tacgccgagc ctgagcccgg
    10861 tgctgacgag gtcaccgagc aggtggtcga ccgtcgcgac gctgagccca gccgtagtcc
    10921 agctacggtg accgagttgg agacccggcg tccggtgaag gatcacccgc gggtggccga
    10981 catcaaccgc atcatcacgg tgcatccgcg cacctacaac gaagctcgca cgatcggcga
    11041 gaacttccgc gacggcattc cggtgatcat gaacctcacc gacatggagg atacagacgc
    11101 gaagcgtctg gtcgacttct ccgccggcct gatctttggc ctgcgtggca gcattgagcg
    11161 catcaccagc aaggtgttcc tgctctcgcc gcacaacgtc accgtggctg ccgaggacaa
    11221 ggagcgcctg gccggcgggt tcttcaacca gtcctgaccc ggcttcggcc aggtcaattc
    11281 ggaaaggcag caaatggccc tcgtcgcgct cctgctcggg tgggccgtat gggcctatct
    11341 ggtcatactg accgtccggc tggtgctggg ctggctgccg gtgctcatcc gtggctggga
    11401 gccgcgcggc tggctttccg tggtggccga gttcgtgtac acggtcaccg acccgccgtt
    11461 gcgggcactc ggtcgggtgc tcaagccact gcgcgtcggt gacctgcaga tcgatttggc
    11521 gttcctggtg ctgtatttcg ggttgctggt cgtccagcgg ctactggccg gctgactctt
    11581 gacccgcggt tcaaccctgc ttctcatcaa tgcgcggtgc ttggcctcag gatccgtgct
    11641 gcactagcct gagaaagccc ttcgacaggc gcgtgactgc gtgcctggca catgacacgt
    11701 gaggtgagta atgaccctga ccctggagca ggtccgccag acccgctttc acctggcccg
    11761 tcgtaacggc tatgagccgg tagacgtcga caacttcgtc gacaaggtcg aggcgacgct
    11821 ctcccagttg aacgaggaga acgccaccct taagcagcag gtggatgcgc tcagctccag
    11881 cgagccttcg agcatcttcg tgcccggcga ctccgctgag gccgaccaac tgaaggctga
    11941 actccagggc cgcagcgacg agctcgcctc cgtccgcggc gaactggaca gactccgcaa
    12001 tgagctgtcc gggaagtcgg ctgagctcga ccaggtccgc gccgaactgg tggccgtccg
    12061 gagcgacctc gacgcggcac gcaacggtga cgtcggccgg tcggacgagc tcgcgcacat
    12121 ccgtggtgag ctggacgccg cccgtgccga ggtcgacggt ctgcgggccg aactcgacgg
    12181 cgttcgcggc gaggccgacc agcttcgcca ggtcgctgcc gatgcccagg ctgccgctgt
    12241 cgcagcgcag ggcgctccgg tcgagccggt cgccccgacg ggcaaggtcg acaacatcgt
    12301 ggtcaccacg tccgctgagg cttccccggc cgtcacccgc ctgctgcaga tggcgaccga
    12361 gcaggccgag cgcctggtcg gcgagtccca ggtcgaggcg cagagaattg tggcgtccgc
    12421 ccgggccgaa gccgaaagcg tcatcgacgg cgccaacaag aaggcccacg agaccctcac
    12481 cgacgcccgc acgcgttcgg atcggatcga gtccgaggcc cgggtcaacg ccgagaagct
    12541 ggtcgcggac gcccgcaaca acgctgactc ggtcaacggc gacgccgatc gccgccgcgc
    12601 cgagctgttc agcgagctcg agcgggagcg ggacgtcctg cgcggcaagg ttgaccacct
    12661 gcgctcgttc gaaagcaact tccgcgccag cctcaccaac cacctgcaga gccaggtgaa
    12721 ggtgttgaac gacgcggtga tcgagccggg agacgttccg gagatcctca gcgagccggc
    12781 tccggaatcg gccaccccga ggttggacgc cctgctgggc gagtagccgc tgccacgccc
    12841 ggccaacggc cgggcgcgcg ctgcgttttg tggccggcca acggtcgggc aggtggtact
    12901 ctccctgctc aaagcatcgg taggaggagc acccaatggc tgcgcgcgcc aacggtatcg
    12961 aaccagcttc cccggctacg ggggttcatt ttccggtagc cgagggcgaa gacccctgga
    13021 ccgaagaaga ggtcgaggag atccggcagg aactgctggc cgacatcgcc cggatggagc
    13081 gggcgatcga ggtggccgag gctggattgg ccgacctctt tgaagagggc agcgatggcg
    13141 ctgggcggga tccggccgac gtcggttcgt ccaacttcga gcgcgaccag gagatgtcac
    13201 tggcgcagaa cgcgcgcgac atgctcgaac aggcccagtt ggcctacaag ctgttcgagt
    13261 ccggcgacta cggctcctgc gaatcctgcg gccagccgat cggcaaggac cggctccagg
    13321 tcttcccgcg ggcaacgatg tgcgtgacct gcaagcagcg ggaggaacgg cgataaccgg
    13381 accgaccgat tcccctgcgc gccggagcct gacctggttg atgttgggtc tgggcgcagc
    13441 cgggctggcg ctggaccagg tggccaagac cgtcgcgatc gcgcggctcg atccgcagca
    13501 gccgatcgcg ctgctcggcg gactggtcac gctgcagctg atccggaacc ccggggcagc
    13561 gttcagcatg ggggagaact tcactgtagt acttaccgga gtggcgatct gcgcgctgat
    13621 cggtgtcctc ggctggctgg tgccccgcgt ccggcacacc ggctgggcgg tggctgtggg
    13681 cttcctgctc gctggcatta ccggaaacct caccgatcgg ctgctgcgtg aacccgggcc
    13741 gttccgcggc cacgtggtcg acttcattca gctgcccaac tttgcgatct tcaatgtggc
    13801 cgacatgttc atcaccgcag cggcggtcct ggtgatctgg ctatcgatga tcacccaggt
    13861 gacaccggcg ggagtcgtca ccaagccgca gccccaagcc cgtggctagc gtctggctgg
    13921 tgccggacgg gctggacggc gaccgggtgg acgcagccgc gtcccggatg accgggctct
    13981 cccgcagccg gatcgaggac ctggccgcgg ccgggggtgt gctggtcaac tcggttccgg
    14041 tggccaagtc gcatcgcgtg cgggccggtg acctgctgga agtggagatc accaccgcac
    14101 cgaccgtttc cgtggttccg gtggaggtgg ccggaatcag catcgtctac gacgacgccg
    14161 acatcgtggt ggtggacaaa cccgccggag tcgccgcgca tcccagtctc ggctgggacg
    14221 ggccgtccgt cgtcgaacac ttggctgcgg ccggcttccg gatctccacc tccggtgcgg
    14281 ccgagcggca gggcatcgtg caacggctgg acgtgggcac gtccgggctg atggtggtgg
    14341 ccaagagtga gccggcctat acggcgttga agcgtgcgtt caaggcgcgc acggttgaga
    14401 agacctacca cacgctggtg cagggcctcc ccgacccgct ggcgggcacg atcgaggcac
    14461 cgatcggacg ccatcccggt gccgactaca agatggcggt ggtcagcggc gggcggcaca
    14521 gcgtcaccca ctaccagatg cttgaagcgt tccgtgcggt gaccctgctg gaggtgcacc
    14581 tggagactgg acgcacccac cagatccgcg tgcacatgca ggcgatccgg catccctgcg
    14641 tcggtgatcc aacctacggc ggcgacccgg tgctggccgc gcaattgggt ctggaccggc
    14701 agtggctgca cgcggtcgaa ctgggcttcg tgcatccgcg cagcggtgac ccggttcgct
    14761 tcacttcccc gtatcctgac gatttagcac gagcgctcgc cctagtcggc gggtgattct
    14821 gggcggggct gagaacgttg ggctagggtg caaccgtgcg tagagcgaaa attgtgtgca
    14881 cgatcggacc ggccactgac tccgccgagg cgatcgaaga actcgtaacc gcgggcatga
    14941 acgtcgcccg gctgaacatg agccacggcg accacttgct acatgccaag cgcgtgggct
    15001 ggatccgcga cgcggcgcgc agcgccaacc gtccgatcgg catcctcgcc gacctgcagg
    15061 ggccgaagat ccgcctgggc aagttccccg atggcccggt gatgctggtg aacggctccc
    15121 ggttcacgat tactaccgaa gacgtgcccg gtgacgccca gcgtgcgtcc accacgctca
    15181 gcaccctcac cgacgatgtc actgccggcg atcagatcct gatcaacgat ggcgccatcg
    15241 aattgaaggt gctcgaagtc actgatgccg acgtggtcac cgaggtggtc gtcggcggca
    15301 tggtctccga ccacaagggg atcaacctgc ccggcgtccc ggtcagcgtc ccggccctga
    15361 gcgagaagga cgaacgcgac ctgcgctggg cgctgcggca cggcgttgac atggtggcgc
    15421 tgtcgttcgt gcgcaacgct gccgacatcg agtcggtgca ccgggtcatg gaggaggagg
    15481 gccggcgggt gccggtcatc gccaagctgg agaagcccca ggccatcgac aacctggacg
    15541 cgatcatcga caccttcgac gctttcatgg tggcccgtgg cgacctaggc gtggagttgc
    15601 cgctggagga cgtcccggcg gtgcagaagc ggatcatcga cgcggcccgg atctgggcca
    15661 agccggtgat cgtggccacc cagatgctgg agtcgatgat cacctcgccg cggccgaccc
    15721 gcgcggaggc atccgatgtc gccaacgcga tccttgacgg tgcggacgcg gtgatgctgt
    15781 cgggggagac cgcggtcggc gagttcgcga tcgaggcggt gtcggtgatg gcccggatca
    15841 tcacgaagac cgaggccgac ggcctggaca agatccggca cctcaggtgg gacccgcaca
    15901 ccgtctccgg ggtgatttcg aaggctgcgg ttgaggtggc cgagcgcacc gaggccaagt
    15961 acctggctgc gttcaccatc tcgggcgaca ccccgcaccg gctggcccgg ctgcgttccg
    16021 ccgtcccact gatggccttc accccgctac ccaagacggc ccaggaactc actctgtgct
    16081 ggggtgtgca gtcctacgtc acgccggagt acaccaacac ggacgcgatg atcgactcgg
    16141 tgcagatggg cctgctggag actggattcg tcagcaaggg cgaccgggtg gtgatcgtcg
    16201 ccggcaaccc cggacggcac acccgccaga ccaactcgct gcgcgtctac gagatcgact
    16261 gaacggcccg gtcggctccg catcgaaccc tccggctcgg tggatctggg gttggccggc
    16321 ttgagacaga actgagtgga cggttcctcg gctacctgac gaggaaccgt ccgctttgtg
    16381 ctggccgcct gctccttgga gttcgatgtc gcggctgggg cacgcccggg acaactggtt
    16441 agcgctacca ctaggccaat taagctttga cctattgaca ggacaaagta ttgtcactag
    16501 tttcgagttg gctggcgagg acgccggcca gggcactagc gacaggcagg ccagtggagc
    16561 acaccgatcc ttctcttccc gacatccaga tcccagagac catgcgcgcc gccgtgctgg
    16621 tggcgccggg gaagcccctg gaggtgcgct ggattcgcac gccgcagccg cgcgccggcg
    16681 aggtgctgat caaggtcacc gcgtgtggcc tatgccattc cgacctgcac gtaatggccg
    16741 gcaagatccc gttcccgacg ccggcggtgc tggggcacga ggtgtccggc acgatcgtcg
    16801 cgctgggtga ggaactgcac cacaccgatc tgaaggtcgg ggacgcggtc tgtggagctt
    16861 tcctgatgcc ctgtgggcag tgcccggatt gcgcgcgcgg ccgcgatgat ctctgcaccc
    16921 cgttcttccg cttcaaccgc ctgcaggggc agctgttcga cgggacgagc cgcctggccg
    16981 acctggacgg caacgtgatc gcccaatact ccatgggtgg cctggccgag tactgcgtga
    17041 tcccagtgac cgcggtcacg ccgatggtgc cgggcatgga cgcggtcgcg gccgcgatcc
    17101 tgggttgcgc ggcgatgacg gcctacggtg cggtgcgccg cggcgccgat ctgcgctacg
    17161 gcgaaaccgt ggctgtgatc ggtatcggtg gggtcggctc gaacatcgtg caggtcgcgc
    17221 atgaactggg cgcgcgcaag gtgatcgcga tcgatgtgga caacgagaag ctggaggcgg
    17281 cgatccgcta cggcgcgtct gatgtgatca actcccggca cctggacgcg cgcgagcggg
    17341 tgttcgacct gaccggcgga cgcggggtcg acgtggccat cgaggcgctg ggcattccgg
    17401 cgacgttcga gcaggcgctc accctgctgg ccgacggcgg ccggatggtg ccgatcgggc
    17461 tggccgccgg cgcccagacc gccgcggtgg agatcaaccg cctggtgcgg cgaggtcagc
    17521 agatctgcgg ctcctacggc gcccggacgc gcaccgacct gccgatcgtg gtcgacctgg
    17581 ccgcgcgtgg cgtcctggac taccggggcg tggtctccga gcgggtctca ctgttcaacg
    17641 ccgaagcgac ctaccgccgg ctggaggaag gccacgtttc cggtcgggcc gtggtggaca
    17701 tggcgctcaa cggctgatct cggagtcggc agccgtcagg tgaaacttcc gcatcgatcg
    17761 gtcccggtct ggccttgatc ggtccgagcg tagacggtgc cgacaaggcc gagtgggaca
    17821 attcacgtcc gctcgaagga aattcggcga cacgccgctg catgcgcctt ctggcaagcg
    17881 attcgcgccg cgagcgggct tcggctgcgc cgcgatggct cctgacctga ccggtgtgcg
    17941 gcgcagaatg agtcggatca gcaccgacac gccgctagaa gtaacaacag ctcacagcgc
    18001 actcgattcg cgccgaaccg gggcgcgggg tcgaacgtct cgcagtcgac caagccggga
    18061 tccggcttca ggcggctggc agaagtggtc gcgttggtgc ggatcagccc acgattcgag
    18121 tcgtaccggg aaggggagtc gaacccctac gcccttgcgg gcagacgggt ttgagccnnn
    18181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngta ccgggaaggg gagtcgaacc
    18301 cctacgccct tgcgggcaga cgggtttgag ccgtccgcgt ataccattcc gccaccccgg
    18361 caggtgctct gcgattgtgc cacaacttgg cgaccgacgt ggtgtgggcg gtaacctcga
    18421 acccgatagg ccgcccgatc aagacccgtt ctgcaggaag caaccatggc aaatagcgac
    18481 gaaaacgacc gtccgacgaa cgagctgcga gttctcgtgg ccgaagacga ggcgctcatc
    18541 cgactcgatc tggttgagct gctgactgac gagggctact cggtgatcgc cgaggccggc
    18601 gacggcgaag aagccgtcga gctggctcgc aagcaccagc ccgacctcgt ggtgatggac
    18661 gtgaagatgc ccaagatgga cggcatctca gcggccgcga tcatcgctga ggagcggatc
    18721 gctccggtcg tgatgctcac cgccttcagc cagcgcgagc tggtcgagcg ggcgcgggac
    18781 gccggcgcca tggcctatgt ggtcaagccg ttcgacgcgt ccgacgtggt gccggccatc
    18841 gagatcgcga tggcgcgctt cgccgagatt cgtgcggtcg aggccgaggt ggccgacctt
    18901 gaggagcggt tcgagtcgcg caaggccgtc gaccaggcca aggggctgct gcaggagggg
    18961 ctcggcctca gcgagcccga agccttccgc tggattcaga agaccgccat ggacctacgc
    19021 aagtccatgc gcgaggtggc cgaaggcgtc atcgaccact ccaaggccgg cggcaagccc
    19081 aagccgctca agaagtaggg gcttattcgg cggcgtccgg cttcgccctg cgcagaaaca
    19141 ccgtgatccg cgccgcgcag atctgctcac cctcgtcgtc gaccagcgag atgtcgtagg
    19201 tggccagcgt gccgccgatg cggatcgcgg tcgccgtccc ggtgatccag cccgaacggg
    19261 ccgaacgcag gtgggtggcg ttgatgtcca ccccggtgac ggcaccctcc gggccgacct
    19321 ctgccgccgc agccatcgag gccaagcctt cggcaagcac gcaggacgcg ccgccgtgcc
    19381 atagcccgaa cggttgcgtg ttgccctcga ccgggcagcg gccgaccgcc cgcgtcgggg
    19441 tcacttcaag tagctctacg cccatcttgt cgtccagtgg actgcgcaag ccggcgaacc
    19501 agtcgggaat cattgggcaa ccttcgcata cccacctgag gtctcgacag gctcgaccac
    19561 catcgactgg cacgaccatc gatcagctga tcagctagga cgtccatgtc agcggccaca
    19621 gctagagttc gggattgtga cgaacccagc tgatcggccc gagaccctgc tcctgatcga
    19681 tggtcactcc gtggcctacc gggcgttctt cgcgctgccg gtggagaact tctcgaccac
    19741 caccggtcag cacaccaatg ccgtctatgg cttcacctcg atgctgatca acgtgctgcg
    19801 cgacgagcag ccgacccacc tcgcggtcgc cttcgacgtg tcccggaaga ccttccgcag
    19861 tgagcagtac gccgagtaca aggccaaccg gtcggcgtcc ccggccgagt tcaccggcca
    19921 ggtgggcctg atcaaggaag tgctggacgc attgaacatc gtccatctgg agcgggacgg
    19981 gttcgaggcc gacgacatca tcgcaaccct cgcccgccag ggcgctgccg ctgggttgcg
    20041 cgtactgatc tgcaccggtg accgcgacac gctgcagttg gtcaacgacc gggtcaccgt
    20101 gctctacccg cgaaaagggg tctccgattt ggcccggctc gacccggcca cggtgctgga
    20161 gaagtacctg gtcacgcccg accgctaccc ggaactggcg gcgctcgtgg gggagagcag
    20221 cgacaacctg cccggcgtcc ccggcgttgg cccgaagacc gccgccaagt ggctggccag
    20281 ctacgacggt ttggagaacc tggtgcggca cgccgacgaa atcggcggca aggtcggcga
    20341 ctcgctgcgc gcccacctcg acgacgtcgt gcgtaaccgc agcctgaacg ccctggtcgg
    20401 cgacctggaa ctgccggtgg ccgtgccgga gcttcagcgt cgcgactggg accgcgaggc
    20461 cgtccactcg ctcttcgacg gactggagtt ccgcgtgctg cgggaccggc tactggagac
    20521 cctgccagcc gcggacgaag agacccaggg cggcttcgag gtgagtggcg agacgctggc
    20581 gtccggcacg gtgcggacct ggctggacac ccatgcgcgc accggtgtgg tcggcctcga
    20641 cgtgaccgga cgctggcgcg cgggggccgg cgacattacc ggcctcgcgc tcgccgcggc
    20701 ggacggtgcc gcggcctgga tcgacgtcac cgagctgtcc ccggacgacg atcgtgcgct
    20761 ggccgactgg ctcgccgatg aagccgcgcc caaggcgatg cacgacgcca agggtccgat
    20821 gctggcgatc tggtcacggg gctgggaact cgccgggctc gccagcgaca cccagctggc
    20881 cgcctacctg ctgcgtcccg accagcgggt ctacgacctc gccgatctgg tggcccgcta
    20941 cctcaagcgt gacctggcgg tcgccggtgg cggctccgcc gagtccgatc agctcgcgtt
    21001 cagcttcgac gacaccgaca gccacgacgc gatggtgcgg gcgcgggcgg tgatcgacct
    21061 ggctgcggcc ctcgaagccg agctcggcgc ccagggcggc gcgagcctgc tggccgaggt
    21121 cgaactgccg ctcacccgca ccttggcgaa gatggagcgg gtcggcgtgg cggtcgacct
    21181 ggaccacctg aaccggctgc acaccgactt cgacgccgcg gtagccgccg ccgaagcgga
    21241 cgcgttcgcg gtgctcggcc acccgatcaa cctgggttcg ccgaagcagc tgcaggcggt
    21301 gctgttcgac gaactcggca tgcccaagac ccgtcgcacc cagaccggct acaccaccga
    21361 cgccgagtcg ctggagtcgc tgtacgcgaa gaccgagcac cccttcctcg cccacctgct
    21421 gcgccaccgc gaccagatcc ggctgcgcca gaccgtcgaa gggctgctga agtcggtggc
    21481 cgacgacggt cgcatccaca ccacctacgt gcagaccatc gccgccaccg gacggctctc
    21541 gtccaccgat ccgaacctgc agaacatccc gatccgcacc gaggccggcc ggcggatccg
    21601 ggaggcgttc gtggtcgggg cgggttacga aaccctgctc accgccgact actcgcagat
    21661 tgaaatgcga atcatggccg aggagtcggc ggacgccggg ctgatcgaag ccttcgcttc
    21721 cgggcaggac ttccacacga tcatggcgtc ccgggtgttc ggggtcgcag ccggtgaggt
    21781 caccccagcg cagcgggcaa agatcaaggc gatgaactac ggcctggcct acgggttgag
    21841 cgcgttcggg ctctcccaac agctgaagat cgagcgatcc gaggccgccg gactgatgga
    21901 ggagtacttc gagcgcttcg ggcatgtccg cgactatctg cgcgagatcg tcgccaccgc
    21961 gagccgcacc ggcttcaccg agaccatcct tggccgccgc cgctacctgc cagacctgac
    22021 ctcgtccaac cggcagcggc gcgagatggc cgagcgcgcg gccctgaacg cgccgatcca
    22081 gggctccgcg gccgacatca tcaaggtggc gatgcttgac gtcgagaccg cgatcgagcg
    22141 ccgcggcctg cgcagccgaa tgctcctgca ggtgcacgac gagctgattt tcgaagtggc
    22201 gccgggggag cgcgaagact tcgaggaact ggtccgggac aagatgggcc acgccgtcga
    22261 gatgcgggtg ccgctggagg tttcggtcgg atacggtcac tcctggcacg cggcggccca
    22321 ctagcgatgt ccgagcagcc ggcccagcgc gaactctggc agcgatccgc cgcccgctac
    22381 gaccaggcca cggcctggct ggagcggcgg atgctggccc gggagcgtga gcgattgatt
    22441 ccccaggctc gtggccggac gctggaggtg ggcgtcggca ctggtgccaa cctgccctgg
    22501 tacccgcacg aggtcgagtt gttcggcgtg gacgcaagcc cggcgatgct cgacctggcc
    22561 cgcaagcgcg cggcacggct cggtcgccag gcgtggttcg agctgggcga tgcggacgcg
    22621 ctcagccatc cggaagcgag cgtcgacaca gtggtggcca cgctgctgct ctgttcggtc
    22681 ccgaaggtcg aatcgaccct ggccgagttc gcccgggtgc tgcgtccggg tgggcggctg
    22741 ctgctgctcg accatgtcga gtcgtcctcg gccttcgtcc gcgctggtca gcggttggcc
    22801 gacctggtca ccagccggac gggggagcgc tggcggcggc gtccgctgcg gcaattgccg
    22861 gacgccggct tcgacgtggt gcagacgcac atcacccgtg cgcgcctact ggaagtcgtc
    22921 gtggccgagc gccgctagca cacagcgaat ccggtcgcca gacgaaagcg cccttcgtca
    22981 agctcagccc agatccgtac ggagcgggtc tcgacaagct ggaccagccg tatcggtaca
    23041 gcccgcatgg ttaagcctgg ctcagggacc ggctatgtca gcgaatgacc gcgagcacct
    23101 gctcctggtc gccgctctcc tcggcccact tgccagtgag ggtgcccagc ggctggcggg
    23161 tgccgggcac gtggctgccg tccagcaccc ggacgagccg agccgactcg ccggccgcgt
    23221 tggagaccag cacgatgccg gaaccctcca cggtcatgct cttctcgccg cgggagaggt
    23281 cggcttcggt gtcaccctca tggatgaccc gcagcagttc gaccacgtag accgggtcgg
    23341 ccaccgcgtc gtaggtgtac cgggtgccca gcaccgagtg ctccatggtg ccgaccaggg
    23401 cgtccgcggc ctcgtccagg ggtgccgagc ggtaggtcag cggcacctgg taggtgaccc
    23461 cggccgagcg caccaggatc gtctcgatgc cgacctctcc gtccgggtcg acgaatcggt
    23521 aggacgcgac ccgggtcagg tcatccgggc tgcccttgaa ccaggactgg gacggcagcc
    23581 aaccggccag cagttccagc ttcgacgggg taagggtggc gtcttcgatc ttggcggtgc
    23641 cgctcatggt ccttggatcc tcctcagggt cgccgcctag cttatcctcg ccgggtcgcc
    23701 agattgaccc tcgctcagcg caaaagctag gctgacggct gcactacgac cggcatgacc
    23761 tcagaccgag tgggtcaggc acgctttcgt ggcccgaccc aggtgtcagc ctggttcccg
    23821 cgatggcacg gggtgcacaa gtcaatctac ataactcaac ccatcggagc cctactacat
    23881 gacctcatct tccgaggcag ccaccgtcgc ggtcgacgac ttcggttctc ccgaagcttt
    23941 cctggcagcg gtcgacgcga ccatcaagta cttcaatgac ggcgacatcg tgaccggcac
    24001 ggtcgtcaag gtcgatcggg acgaggtcct gctcgacatc ggttacaaga ccgaaggtgt
    24061 cattccctcc aaggaactct ccatcaagca cgacgtggac ccgttcgacg tggtcagtgt
    24121 cggcgatgtg atcgaggccc tggttcagac caaggaggac aaggaaggcc gcctgatcct
    24181 gtccaagaag cgggctcagt acgagcgcgc ctggggcacg atcgagaagg tcaaggaaga
    24241 ggacggcgtg gtcaccggcc gcgtcatcga ggtcgtcaag ggcggcctga tcatcgacat
    24301 cggcctgcgc ggcttcctgc cggcttcgct ggtggagatg cgccgcgtcc gcgacctgca
    24361 gccctacgtc ggcgctgagc tcgaggcgaa gatcatcgag ctcgacaaga accgcaacaa
    24421 cgtggtgctg tcccggcgcg cgtggctgga gcagacccag tccgaggttc gccacacctt
    24481 cctcaaccag ctcaccaagg gccagatccg caagggtgtg gtttcgtcca tcgtcaactt
    24541 cggcgcgttc gtcgaccttg gcggcgtgga cggcctggtg cacgtctccg agctgtcctg
    24601 gaagcacatc gaccacccgt ccgaggtcgt cgaggttggc gacgaggtca ccgtcgaggt
    24661 gctgagcgtc gagatggacc gcgagcgcgt ctccctgtcg ctgaaggcga cgcaggagga
    24721 tccgtggcag accttcgcgc gcacccacca gatcggccag atcgtgccgg gcaaggtcac
    24781 caagctggtg ccgttcggtg cgttcgtccg ggtcgaggag ggcatcgagg gcctggtgca
    24841 cgtgtccgag ctcgccgagc gccacgtcga gatccccgag caggtcgtca ccgtcaacga
    24901 cgaggtcctg gtcaagatca tcgacatcga cctggagcgt cgccggatct cgctgagcct
    24961 caagcaggcc aacgagggtg tcgacatcgc cgcggacgac ttcgaccccg cgctgtacgg
    25021 catgtcggcg tcctacgacg agcagggcaa ctacatctac cccgagggct tcgatccgga
    25081 gacccaggag tggaagcccg gttacgaggc gcagcagcgc gcttgggagc agcagtacgc
    25141 cgaggcccag gcccgttggg aagcccacaa gaagcaggtc gaggaggccg aggctgccga
    25201 cgtgcaggcc gcggtcaccg aggccaacac cgccgcctac tccgcgggtg cagccgacac
    25261 gtcggccccc gagggttcgc tggcttccga cgaggcgctg caggcgctcc gcgagaagct
    25321 gaccggcggt cgctgaccca gccagtaacg acgtgcccct caccgcttgg tggggggcac
    25381 gtcgcttttt catcggctct ggcaaacttg gcggcatgta cccccagggg gcggccgtga
    25441 cggacaaccg cttcgagaac tatccgcccg cgccgacgtt cgctgctcat gacgatgtcg
    25501 ctgccgagcc cgtcagcgga gtggtgaccc cggcgaagcc gtactcgatt ccgccgcgga
    25561 caaggcgacc cagtcgtcgg gtcatgctcg ccttgatgat cggtgttccg gttgcggtcg
    25621 ggctgggcat cgcactgaac gtggacgacg acggcgaccc cgaagtggac actggctcgt
    25681 tcctgctggc cgaccacgag atcagcgtgc cgctgaactg gacgtacgag ctgtcagcgg
    25741 acggcgaagc tgcgacgctg acgagagccg gcaataccgt gaagatcgcc gcagtgcgcc
    25801 ggttcgatgg cgagcaggcc atcaacgtga tcggcgaaca gaccggggcg ttggtgcccg
    25861 actacacgag tcggcggatg caggaaccgg tggattcatc gtccggtcgc ttcgaccgcg
    25921 cgaccctgac cggaaccgca gaactccatg gtgtccacgt ccggattctg tccaacctgt
    25981 ggctcaccga aggtgaaggc ctgattgccg tccggctgct caccgaggcc tcgcgcagca
    26041 gttacaccac cgaggcggcc gagatcgtgc gtgcagtggg agagaacttc tgatgtccga
    26101 ccagaacccc acgcagttcg agaactaccc gaccgagccg ggcgacatgg gtgcggtcac
    26161 cgagcccggt ccaggcgcgt cgtcggcgcc gctggtggta ccggcaccac cagcacagat
    26221 cgctgactgg tcggccgaca ccgagaagtc agatggcacc gcacggcgtg tggtgatcgg
    26281 atcgatcgtg ggcatcgtgg cgctgggctt catcggtagc gtcagtcgga actccgggga
    26341 tggcgaaagc tccgaggttt cgttcacagc ggctgcaccc gaggagacga tctacgagta
    26401 cgaagaggaa ccggagtccg gcactcccct ggagctgggg ggctacgccg cgaccctgcc
    26461 ggacggctgg acgctcgaca acaccagcga tcagctggcc gtggcgacct gcggcacgaa
    26521 ccgggtgctg gtgcggctgc tggacaactc ggggctgagc gccaccgagc ggatcaccgg
    26581 agcgatgaag ggcaggctcg gctccttcaa gggaagcctg gacgaaccgg aggacgtcag
    26641 cttcggcagc acggacgcgg cgcgcatcgt cggtacgggc aagatcggga agacggcggc
    26701 acgcctgatc ggtcagctct gggtggacgc cgacgacaac tcgctgctgg tggtgcagac
    26761 gctcaccgcg aagcccgact ccgagcgcgc gctgcaggcc caggacatcg ccgaccaact
    26821 gagctcgaac ttcggatgag cgggtcgacg gtgctggtgg ggctcaccgg cgggatcgcg
    26881 tccgggaagt cgacggtcgc ggatctgctg gccgcgcatg gcgcagtggt gatcgatgcc
    26941 gacctgctgg cccgggaggt ggtcgaaccc ggttcgcccg gtctggtcgc ggtggttgag
    27001 cggttcggga ccggcgtgct gcgcgccgac ggcagcctcg accggcccgc gctcgggcgc
    27061 ctggtcttcg cggacacgga cgcgcgccgc gacctggaac gcatcatcca cccggcggtt
    27121 cgggagcgcg cggccgagtt ggtcgcgtcc gctgagccgg ggtcggtggt cgtgcagatg
    27181 atcccgctgc tggtcgagac cgggcagcag gacgccttcg acctggtggt cgtggtcgac
    27241 gtcgacccgg gcgtgcagtt ggcccgagtc cgcgtccgcg acggtcttag cgaggcagag
    27301 gcgctggccc ggatcggtgc ccaggccagt cgcgaagcgc gactggcggc cgccgatgtg
    27361 gtgatcacca acaacggtgg tctgggcgaa ctgcgcgagc aggtggacaa gttgtggcgc
    27421 gaattcacca ggaacgaacc ccccgcagcg cccggttggc gggcgaaacg gaatgtcaga
    27481 ggggacgcat aggctcttcc tcgtgcgacc gatcactgat gtgcagcgtc gggtggcccc
    27541 cttcaaggtg gtcgccgact tcgagcctgc cggcgaccag ccggcggcga ttgaggagct
    27601 cgaacgccgg ctgaccgccg gtgagcagga cgtcgtgctg ctgggcgcca cgggcaccgg
    27661 taagaccgcg acgatcgctt ggctggccga gcgggtgcag cgtccgatgc tggtgatgca
    27721 gcccaacaag acgctggctg cgcagttcgc gaatgagttg cggcagttct tccccgataa
    27781 cgccgtggag tacttcgtct cgtactacga ctactaccag cctgaggcgt acgtgccgca
    27841 gaccgacacc tacatcgaga aggactcgtc gctgaacgag gaggtggagc ggctccggca
    27901 ctcagccacc aactcgctgc tgaccaggcg cgatgtgatc gtggtcgcga cggtctcggc
    27961 catctacggg ctgggcacgc cgcaggagta cgtggatcga atggttcggc tccgggtcgg
    28021 cgaggagacc gaccgggacg gcctgctgcg acgcctggtc gacatccagt acgtccgcaa
    28081 cgacctggcc gcgacccggg gcacgttccg ggtgcgcggc gacacggtcg aggtgttccc
    28141 gatgtacgag gagcacgccg tccgggtgga attcttcggc gatgagatcg aggcactgag
    28201 cacgctgcac ccgttgaccg gcgagatcct cagcaacgac caggagatct acgtgttccc
    28261 cgcctcgcac tacgtcgccg gtccgcagcg catggaacgc gcgatcgacg gcatcgagag
    28321 cgagttggca gtgcggctcg ccgacctaga ggccaacagc caactgctgg aagcgcaacg
    28381 gctgcggatg cgaaccggat acgacatcga aatgatgcgc cagatcggta cctgttccgg
    28441 catcgaaaac tactcgcggc acattgatgg ccgcgcgcag gggagtgcgc ccaactgcct
    28501 gctggactac tttcccgagg atttcctgct ggtggtggac gagtcccacg tcaccattcc
    28561 ccagatcggc ggcatgtacg agggcgacat gtcccgcaag cgcaccctgg tcgagcacgg
    28621 cttccggctg ccgtccgcga tggacaaccg gccgctgcgc tgggaagagt tcgtcgaccg
    28681 gatcgggcag accgtgtacc tctcggccac gcccggaccg tacgaaatgg ccaagtcgga
    28741 cggttacgtg gagcaactga tccggcccac cggcctggtc gaccctgaga tcgtgctcaa
    28801 gccgaccaag ggtcagatcg atgacttgat ggccgagatc cgcgcgcggg cggagaggaa
    28861 cgagcgggtg ttggtgacca cgctcactaa gaagatggcc gaggacctca ccgactacct
    28921 gatggagaac ggcgtccgca cccgctacct gcactccgag gtggacaccc tgcggcgaat
    28981 cgaactgctc cgggaactcc ggctcggcgt ctatgacgtg ctggtcggca tcaacctgct
    29041 gcgcgagggc ctcgatctgc ccgaagtgag cctggtggcc attctggacg ccgacaagga
    29101 aggcttcctg cgcagtgagc ggtcgctgat ccagacgatc ggacgcgccg cgcgaaacgt
    29161 ggccggccag gtgcacatgt acgccgacaa gatcaccccg tccatggccg cggcgatcga
    29221 cgagaccaat cggcggcggg agaagcaggt cgcctacaac acggaacgtg ggatcgatcc
    29281 acagcccctg cgcaagaaga tcgcagacct caccgacctg ttggccaggg aggatgccga
    29341 taccgaaggt ctggtcagcc gggccggcgg taggcgtccg gtcagcccga cgccgatgtc
    29401 gacgcagaag aaccgcgacc tcggcgcgat gccgtccagc gagctggccg ggctgatcag
    29461 tgaactgaac gagcagatgc actcggccgc ggcggaactc cagttcgagc tggctgcccg
    29521 gttgcgcgac gagatagccg acctgaagaa gacgctgcgg cagatgctgg aggccaccaa
    29581 gtgaggcctc gcccgcagcc ggtggttggt gtgtcccgga agtgccgtgg gagactgctg
    29641 cgatgcatgt gacgctcgag gtctggctgg taacgatcgc gatcatggcc gcggtgctga
    29701 tcgtcgacct ggtcgtgatc ggccggcgtc cgcacgaacc gtccatgcgt gaagccggta
    29761 ctgcgatcgg catcttctcc gggctggcgg tgctgttcgg gctgggcgtg tggtggttcg
    29821 ctggtgcccg cttcgccggc gagttcttcg ccggctggct gaccgagtac agcctcagca
    29881 tcgacaacct gttcatcttc gtcatcatca tggccaacct gcgggtgccc aggcagttgc
    29941 agcagttcgc actgatggtc ggcatcgtgc tcgccctcgt gttccgtggc atcttcatcg
    30001 ccctcggcgc ggcggcgatc gagcggttca gttgggtgtt cttcctgttc ggtgccttcc
    30061 tgatctggac cgccgccaag ctctacatcg actatcgcca gcacgatgag gaggaagagg
    30121 ccaccgagaa cgcggtgatg cgctgggtga agcggacgct gccgtccaca cccgatttcc
    30181 acggcaccaa gctgacggtc gtggcggcgg gcaagcggct gatcacgccg atgttctttg
    30241 tgatcgtggc cctcggcacc accgacctgc tcttcgccct ggactcgatt ccagcgatct
    30301 acggtctcac ccaggagccc tacctggtct tcgcggcgaa cgtgttcgcg ctgatgggcc
    30361 tgcgccagct gtacttcctg atcggcgggc tgctgcaacg gctggtctat ctggcggttg
    30421 gactctcggt ggtgttggcg ttcatcgggg tgaagctggt gctgcacgcc atgcacgagt
    30481 accactggga cgagcggctc ggcttcaacg gcgagatccc gatctgggcg tcgctgacct
    30541 tcatcctggt aaccctcggg gtgaccaccg tggccagcct ggtcaagtcc cggcgcagcg
    30601 acgcactacc cgagtagggt cggctggaat ccgtccgcgc cggtcaccca gtccggagtg
    30661 ccgcgtccgc tgagccaagc ccagagccgg acgtcactgc cgctgaccac caaccggtcc
    30721 gcgccgtggc cgagttcggt caccagggaa tcggcctcga tccggagggc gggcaggtcg
    30781 gcgtcctgca ggcagtcgat gacccagcgc agtaaccagg ccgctgccgc cggatcgatc
    30841 agttcgggtg cgaagtcgaa gtcgaggtcg agatggtgga tgcagacctc gtgcagccgg
    30901 gccagggcca gtgccgagag ggggtgcctg cgaccgtgga tgtccacctc gtggtcccag
    30961 tcggtggtgc cgtcggtgat ccggtgcaac gccccggcgc tggtgtcgag atcgatctgc
    31021 agggcgagcc cgtcgcggtc cgcccctacc tcaagcgccg cgatccgatc gctgcggacc
    31081 aagggctcag gttggggcac gccggcggag acagcttcga gcacccctcg gacgtggtcg
    31141 gcgttgcgcg ccagatgagt ggctacgtgc gcccggctcc agccgggaag cgtggtcggc
    31201 gcgtgccagg aatcgtcgtc cagcacgatc gtgtagccca gcagacgctg cgtcgcctcg
    31261 gcctgccaac ggtgcacatc gtcggtcgga accgtgccga tgtgccattt catgtgacca
    31321 acctacgggt caggggcttc gggagccacc cccggaacgc tggcgcgagc tgtgtcggaa
    31381 ccaccatcta gcatgttcga cgtgactgac cggctgatcg tgcgcggagc gcgcgagcat
    31441 aacctccgaa acatcaacct cgacctaccc cgggacgcga tgatcgtgtt cacgggcctc
    31501 tccggctcgg gcaagtcgag cctcgccttc gacacgatct tcgccgaggg gcagcggcgc
    31561 tacgtcgaga gcctctccgc ctacgcccgg cagttcctcg gccaaatgga caagcccgac
    31621 gtcgacttca tcgaaggcct ttccccagcg gtctcgatcg accagaagtc caccagccgg
    31681 aacccgcgct ccacggtcgg caccattacc gagatctacg actatctccg cctgctgtat
    31741 gcccgggtcg gccggcccca ctgcccggag tgcggcgagc cgatcagccg gcagtcgccg
    31801 cagcagatcg tcgaccggct gatggggcta cctgagggca cccggttcca ggtgctcgcg
    31861 ccggtcgtcc gcgggcgtaa gggtgagtac gtcgacctgt tcgcacagct cgctggtcag
    31921 gggctctccc gcgtccgagt ggacggggaa gtgcaccagt tgctgcagcc ccccacgctc
    31981 gccaagcaga agaagcacaa catcgacgtg atcgtcgacc ggttggcggc caagcccagc
    32041 atcaagcagc ggctcaccga ctcggtggag actgcactcg gtctggccgg cggagtggtg
    32101 gccatcgact tcgtcgacct ggacgaccac gacccgaagc gggagcgccg ctactcggag
    32161 cggctggcct gccccaacga gcacgacatc tcggttgagg aactcgagcc gcggcagttc
    32221 tctttcaacg ggccgtgggg tgcctgtcca gcctgttccg ggctgggcac gcggatggaa
    32281 gtggacgtcg acctggtggt gcccgatcca agcaagaccc tcgcgcaggg cgcaatcacg
    32341 gtctggacca cgccctacgt ggcgggctac ttccagagcc tgttcaaagc actcggggca
    32401 caggagggct tctcggtcga taccccgtgg gaggagttgc ccagccgcgc ccagagcctg
    32461 ctgctgatgg gcgcgccgca cgcgatcacc gtgcacgcca cctcgcggca cggacgcgag
    32521 cacagctatc gcgccaagta cgagggtgcg ttgccctaca tccggcggcg gcacgccgag
    32581 gccgaatcgg agaactcgcg ggaacgcttc gcgggctaca tgcgggaagt gccctgcctc
    32641 acctgtaacg gcgcccggtt gaagcccagt tcactggcag tgaccatcga cggacgcaac
    32701 atcgccgagg tgtcagccat gtctatcgac gaggtggccg ggttcatgcg cggtctcaaa
    32761 ctgcccgagc gggaggccca gatcgccgag cgggtggtca aggaggtcaa cgagcggctg
    32821 cggttcctac tagacgtcgg cctggactac ctgtcgctgg cgcgtccggc gggcagcctt
    32881 tccggcgggg aggcgcagcg gattcggttg gccacccaga tcggttccgg tctggtcggc
    32941 gtgctctatg ttctggacga gccatccatc ggcctgcacc agcgggacaa ccgaagactg
    33001 atcgacaccc tcgtgcggct ccgcgacctt ggcaacaccc tgatcgtggt cgagcatgac
    33061 gaggacacca tccgcacagc cgactgggcg gtcgacatcg gcccgggtgc gggggagcat
    33121 ggcggcgagg tgctcgtgtc cgggccggtg gccgaactcc tggcgcaccc gacctcgctg
    33181 accggcgcct acctggccgg acgccggcgc atccccatgc cgagccggcg acgtccggcc
    33241 actggcaagg cgatcacggt gcacggcgcg gccgaacaca acctgcgcaa tgtgacggtc
    33301 gagttcccgc tcggccagtt gatcgcggtc accggcgtct ccggatcggg caagtcctcg
    33361 ctggtgaaca gcatcctgta cacagcgctg gcgaagaagt tgtacaacgc gcacaccgtt
    33421 cccggacggc accgcacgat cacgggcatc gagcacatcg acaagagcat ccatgtcgac
    33481 cagtcaccga tcgggcggac gccgcggtcg aacccggcca cctacaccgg cgtgttcgac
    33541 cacatccgtc ggctgttcgc agagactccc gaggccaagg tgcggggcta ccagccggga
    33601 cggttctcct tcaacgtgaa gggcgggcgc tgcgagaact gcgccggcga cggcaccatc
    33661 aagatcgaga tgaacttcct gccggacgtc tacgtgccct gcgaggtctg ccacggcgcc
    33721 cggtacaacc gggagacgct ggaggtgcac tacaagggca ggacgatcgc cgaggtgctg
    33781 gacatgccga tcgaggaggg cgtggacttc ttcggcgcca ttccggcgat cgcccggcac
    33841 ctgcgcaccc tggtggatgt cgggctcggc tatgtgcggc tcggacagcc agccaccacg
    33901 ctgtcgggcg gggaggccca gcgggtgaag ctggctgccg aactccagaa gcggtccacc
    33961 gggcgcacga tctatgtgct ggacgagccc accaccggct tgcacttcga ggacatccgc
    34021 aagctgctcc aagtgctcca gtcgctggtc gacaagggca acacggtggt cgtgatcgaa
    34081 cacaatctcg acgtgatcaa ggcggccgac tgggtgatcg acctcggccc cgaaggcggt
    34141 tcccgcggcg gcatggtcgt tgccgagggc acacccgagc aggtcgccga caacccggcg
    34201 tcgttcaccg gagcgttcct gaaagcggtg ctggacggcc agcgcgtacc tgtgggcgaa
    34261 tcggaaccag ccctgctcgc ctaggagcct gttaagagat gctcaggctg ttgccgttca
    34321 cggtgaccgt ggccttggtg agtcccttgg tggccggacc ggtgagtacc gagccgtcgg
    34381 cgagagagaa ggtgctgccg tggcgtccgc agatgatcgc ggcgtcgctg acgtcctgca
    34441 cgggcaggtt ctggtgggtg cacaggtagc tgaacgcctg gaagtcgccc tcggtggcct
    34501 gcgtcactac gtactggccg acgatcttcc caccgccaac gggcacatcg gccgcgctta
    34561 cggtgttccc atcggccgcg gcgccaccgg acgcgccccc gccggctgct tccgttccgg
    34621 aactcgacgg cgcacagccc gacaggcaga ccgcgagggt ggtgccgccg atcgtgatca
    34681 gcgcggcgcg tcgggacgga agcgggttgg tcatcggagc tcctcttagt tgccgtttca
    34741 acagtctagg gtcggcgggt tcgtgctggc tgtggttggg ctgtgaatgc tccgcccttt
    34801 caagccctcg tttcggctgg tcgagcttgt cgagacccct cgtcaggctc agggatcgct
    34861 gatcaggctc ggggaccgtt ccgtcggcgg tggtgcctat gctgacttac gtgccggaac
    34921 gtccggtggg aggggaggtg gtcgtggctg atccggccag ctaccgtccc gcgcccggca
    34981 ccattccgac cggcccgggc gtgtaccgct tcagtgaccg tgaaggtcgc gtgatctacg
    35041 tcggcaaggc catcaacctg cgtcagcggc tcaactcgta cttcgccgac ccgtccgcgc
    35101 tgcatttccg tacccagacc atggtgcgca ccgctgctgg ggtcgaatgg acgctggtgc
    35161 gcaacgaact cgaagccctt cagcttgagt acacctggat caagcagttc gacccgcgct
    35221 tcaacgtcaa gtaccgcgac gacaagtcct acccctgggc cgcggtcacc tggtcggaag
    35281 agttccccag ggtcttcgtg ggccgcggca gcaagcgcaa gggcgtccgc tacttcggtc
    35341 cgtatgccca ggcttgggcc atccgggaca cgatcgattc gctgctgcgg gtcttcccga
    35401 tgcggtcctg taccaacggc gtctttcgca gcgcccgcgc cagcggccga ccgtgcctga
    35461 tgggctacat cggcaagtgc gccgcccctt gcgtggaccg gatcgacccg gacgctcacc
    35521 gggtgctcgt cgaggacgtc tgcgacttcc tcgccggacg gacggcagcg ctcacccggc
    35581 gcctcgaagc cgagatgcgg atcgcggcca ccaacctcga atacgagcgc gccgccgtga
    35641 tccgcgacca actggtagcg ctcaagcagg ccagcgagcg caatgcgata gtgctcggcg
    35701 acggcacgga cgccgacgtc attgccctgg ccgaggaccc actcgaggtc gccgtccagg
    35761 tcttcaacgt gcgggacgga cgcgtgcgcg gcgaacgcgg ctgggtggcc gagcgcagcg
    35821 acgatgccga caccggccgg ctgctggaac agttcctgct gcagctctac gcagaggacg
    35881 ccgagtcgat tccgcccgag atcctgctgc cggccctacc cacgtcgcca gaggtcttgg
    35941 cccaactgct gggcgacgag cgcggcacca aggtgaagct gaaggtaccc aagcgcggcg
    36001 atcgagccgc gctgctggag accgcagccc ggaatgcaac ggacgcgctg acgctgcaca
    36061 aggtgcgccg ggccagcgac ctgtccaccc gcaaccgggc actggaggaa ctgcaggagg
    36121 ccctcggcct gcccgaagtg ccgctgcgga tcgagtgcta cgacatctcg cacactcagg
    36181 gcagcgaggt ggtcggctcg atggtcgtct tcgaggacgg tctggcgcgc aagagcgaat
    36241 accgaaggtt catcatccgc accgtcgagg gcagcaacga cgtcggtgcg atgcgcgagg
    36301 tgatcacccg ccggttcaac cggctgctcg ccgagaacga gcgcaccggc gaggagtccg
    36361 ggccgctgct ggtcgacccg accactggcg cgccgcgcaa gttcgcctac ctgccgggac
    36421 tggttgtggt ggatggcggc gctccccagg tggccgcagc ccaggctgcg ctgagtgaac
    36481 tcggcattca gaacgtcgca ctttgcggcc tcgccaagaa actggaggag gtctggctgc
    36541 ctggtgagga gtacccgctg atcctgccgc gcgccagcga ggggctgtac ctgctgcagc
    36601 gggtccgcga cgaggcgcac cgcttcgcga tcacccacca tcgcagccgt cgcagcgcgt
    36661 ccatggtcga gtctgcgctg gacgacgtcc ccgggctggg ggagatccgg cgcaaggccc
    36721 tgttgaagta cttcggctcg ctgcgaaagc tgcgggcggc aacggtcgag caactggccg
    36781 aggtacccgg cgtcggcctg aagaccgcag ccgcggtgaa ggaggcgctt gctcagcatg
    36841 ggccggccga ggcgatcaac ctgaccaccg gcgagatcat ccagacctga ccggtgtgga
    36901 cgcagcggcg gtgataatga agccatgcaa tcgacgcccg cagtccgact actggtgctg
    36961 accggccttt ccggatccgg gaagcggacc gcagggcaca ccctcgaaga ccttggctgg
    37021 ctggtggtgg acaacctgcc gccgagcctg ctgcccgacc tgatcgaggt gagccggcag
    37081 cgcgagttca accgcgtggc cgtgctgctg gacgtccgca gccggtcgga gttccagcag
    37141 attccggaag cgttcgccac tctcggtgaa gccggaatcg tgccgcagat cgtgttcatc
    37201 gatgccagcg acgagacgat cgtgcgccgg caagagtcgg tacgccggcc gctcccactg
    37261 cagggccccg gacgcatcct tgacggcatc cgccgcgagc gcgagttgct ggccggcctg
    37321 cgagccagcg ccgacctggt catcgacacc agcgcgctga acgtgcatga cctagccgcc
    37381 agggtcacgg tggccttcgc ggacgaggac gaggcgcaac tcaagctcac tctgctgtcg
    37441 ttcggcttca agaacggcat cccgatcgac gccgacatcg tgctggacgt gcgcttcctg
    37501 ccgaacccgc actgggtgcc ggccctgcgt ccgcagtcgg gcctgtcgca gcccgtggcc
    37561 cactacgtgc ttggccaacc tgatgcggaa cccttcctcg accatgtgct cggcatgatc
    37621 cggctgatga tcccgggcta cgtccgcgaa ggcaagcggc acgccatggt ggcgatcggc
    37681 tgcaccggcg gcaagcatcg ttcgaccgcg atcgtggagg agatcgcccg tcgcctggcc
    37741 gtggacggga ccccggccga ggtggtccac cgcgatctgg gcagggaatg aacttcacca
    37801 aaccgccggc ggtggttgcc ttcggtgggg gacacggact cgcggccagc ctgagtgcgc
    37861 tgcgccagct gacccatcgg ctgaccgcgg tggtgacggt cgccgacgac ggtggatcct
    37921 cgggccggct ccgcgacgag ttcccgatcc tgccgccagg cgacctgcgc atggcgctcg
    37981 ccgcgctctg cggagatgac gaggtcggtc gtgcgtgggc cgaggtgctc caatcacggt
    38041 tccctggcga gggtccattg ggcgggcaca gcatcggcaa cctgctgatc gccgggctct
    38101 ggcagcagcg tgccgacccg gtagccggcc tggacatggt ggcgtccatg ctgcacgtcg
    38161 agggtcgggt gctgccgatg gcagcggttc cgctggtgat cgaggcggac gtgatcggcg
    38221 ccgaccccga gtacccagac gacaccatgg tggtggtggg ccaggaagag gttgccctgg
    38281 ccaatggcga catcggggac gtccggctgg tgccggccaa cccgccggcc gtgcctgagg
    38341 cgctgcgcgc gatcgccgaa gccgaccacc tggtgttcgg gcccgggtcc tggtactcct
    38401 cggtgatccc gcacgtgctc gtccccgaac tgctggatgc gatcctcgcc tcgccggcac
    38461 agcgcatcct cacgctcaac ctggtgccga ccgcggacga gacgccggcc tacaccgcgt
    38521 cccggcatgt ggaggtgctg gccgagcacg caccgggcct tcgcctggac gcggtggtcg
    38581 ccgatccttc gttcgcggac ggggacacgc acctggcaac ctacgtccgc tccctcggcg
    38641 cccgcctggt ggtggcaccg gtgcggatgc gggacggttc cccccggcac gatccgcacc
    38701 tgctgtccgc ggtgtatgcc gggatcatgg gtctgtaagc tgagccccac tcccacccag
    38761 gctcgatcga gaggtagcag cgcgcagatg gcgatgacgc agcaggtgaa ggccgaactg
    38821 gccaccctcg aggtgaccaa gccggagctt cgcagggccg aagtcgccgc gttgctgcgc
    38881 ttcgccaacg gcctgcacat cgccggcgga cggattgtgg tcgaggcgga actcgacacc
    38941 ggcgctgcgg cccgccgcct gcgcacggcg atcggcgacc tgttcgggtt caactgtgag
    39001 ttcgtggtga tcgccggcag tggcctgcgc cgcggtgcgc gctacctcgt gcgcctgatc
    39061 cgcgacggtg aagctctggc ccgccagacc ggactgatcg attcccgcgg ccgccccgtc
    39121 cgcggcctgc cggtgcaggt ggtcagcggt ggcctggacg tctccgtcgc tgcctggcgc
    39181 ggggccttcc tcgcccacgg ctcgctcacc gagccgggcc ggtcgatggc gctggaagtc
    39241 acctgccctg gatcggaggc cgcgctcgcg ctggtcggcg cggcccgtcg gctcgggatc
    39301 agcgccaagg cgcgggaggt ccggggcatc gaccgagtgg tgatccggga cggggacgcg
    39361 atcgcggcga tgcttacccg gcttggcgcg cacgagtccg tccttgcctg ggaggagcgc
    39421 cggatgcgcc gggaggtgcg ggcgtccgcc aatcggctgg ccaacttcga cgatgccaac
    39481 ctgcgccgct cggctcgggc ggcggtggcg gccggtgccc gggtgaagcg ggcactcgag
    39541 atcctgggcg acgacattcc cgagcacctg ctggaggccg gccgactgcg gctggacaac
    39601 aagcaggcga gccttgagga actcggccag ctgcacgtcc cgcccttgac caaggacgca
    39661 gttgccggcc ggatccggcg gctgctggcc accgcggaca agcgcgcggc agagctcggc
    39721 attccgaaca ctgaggtcgg cctgagcccc gagatgttag acgacgacgg ataaccggcg
    39781 tcagccggtt
//
LOCUS       DAOPRQ010000013        39104 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_792_length_39104_cov_12.452662, whole genome shotgun sequence.
ACCESSION   DAOPRQ010000013 DAOPRQ010000000
VERSION     DAOPRQ010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 39104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39104
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_792_length_39104_cov_12.452662"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(114..186)
                     /locus_tag="PKJ61_02770"
     tRNA            complement(114..186)
                     /locus_tag="PKJ61_02770"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(151..153),aa:Thr,seq:ggt)
     gene            complement(263..556)
                     /locus_tag="PKJ61_02775"
     CDS             complement(263..556)
                     /locus_tag="PKJ61_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007077132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HOC12782.1"
                     /translation="MPSMAELADSILLVADAIPSGRVSTYGDIARVVGCGPRLVARVL
                     AGSGGETCWWRVIRADGTIAAPLVAEASERLIGEGVSVRAGRVDLSRYRAELD"
     gene            618..2009
                     /locus_tag="PKJ61_02780"
     CDS             618..2009
                     /locus_tag="PKJ61_02780"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007078398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HOC12783.1"
                     /translation="MTEYRIEHDTMGEVRVPASALYAAQTQRAVENFPISGRGLSSHH
                     IAALAQIKRAAALANAELGVLSPDVADAIVAASEEVIAGQHDDQFPIDVFQTGSGTSS
                     NMNANEVVANLASRGLGRPVHPNDQVNASQSSNDVFPSSIHIAAVQAIHETLFPGLET
                     LAASLEAKSAEYASVVKSGRTHLMDATPITLGQEFSGYATQVRYGIERVRAALPRLCE
                     LPLGGTAVGTGINTPDGFSARVIELIAANLSLPFVEAPNHFEAQAAQDSLVETSGALR
                     TIAVSLTKIANDLRWMGSGPRTGIGEISLPDLQPGSSIMPGKVNPVLPEATVMVAAQV
                     IGNDAAIAFAGAQGNFDLLVMLPVMARNLLESITLIGNVSRVLATRCVDGIEANVERC
                     RQLAESSPSIVTPLNRIVGYEAAAKIAKHSVKANLTVADAVRDLGYVERGEITEEQVA
                     TALDVTTMIGVNR"
     gene            complement(1999..2613)
                     /locus_tag="PKJ61_02785"
     CDS             complement(1999..2613)
                     /locus_tag="PKJ61_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011758047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HOC12784.1"
                     /translation="MPPTSQLLAFLAVAVVLILIPGPSVLFVVSRAVAMGRGAAVRTA
                     LGNAIGTLVLVAAVAFGLGALIAAATISYEIIRLAGAAYLIYLGVQAIRHRRELAVPD
                     AAPSNRRVLAEGFVVGLTNPKTAVFFAAMLPQFIDPAQPALPQLLLLGAVFVAIAALC
                     DSLWGLFAGTARSWFASSPRRLRRLSATGGGVMIALGARLAISD"
     gene            3077..3412
                     /locus_tag="PKJ61_02790"
     CDS             3077..3412
                     /locus_tag="PKJ61_02790"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HOC12785.1"
                     /translation="MTDDRVARRLEDLAQIGAEAAYLVAKGADAYLADTMDGALLQDA
                     GERILIKIATVAEKLPNDFKAAHPDIDWAGINRMRNLVAHHCGKVNDDLMWQALTGRI
                     PNLVNRIVA"
     gene            complement(3496..3577)
                     /locus_tag="PKJ61_02795"
     tRNA            complement(3496..3577)
                     /locus_tag="PKJ61_02795"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3541..3543),aa:Tyr,seq:gta)
     gene            4489..4980
                     /locus_tag="PKJ61_02800"
     CDS             4489..4980
                     /locus_tag="PKJ61_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012923767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HOC12786.1"
                     /translation="MAAENSFDIVSKVDHQEVDNALNMAAKEVRNRFDFKGTDASIRW
                     SGEAIEMEASGEERVKAILDVFQTMLVRRKIDLKSLDAGEPRLSGKMWKLTASVKQGI
                     SRENAAKIAKLIRDEGPKGVRTQVQGEELRVFSKKRDDLQVVQQLVRGADYDFAVQFD
                     NYR"
     gene            5015..6046
                     /locus_tag="PKJ61_02805"
     CDS             5015..6046
                     /locus_tag="PKJ61_02805"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOC12787.1"
                     /translation="MGVTPYLRLDLDVLERNLDAMAAHARTLGLALRPHAKTHKCPQI
                     ADMQLARGAVGLTVATIGEAEVFAAAGAGDLFIAYPLWVDADDADRLHRLAEGGVRLR
                     VGCDSAEAGAQLRGLPVELVVEIDSGHHRSGVAPAAAGTLASGLADLGMAVAGVFTFP
                     GHSYSPDEASAAAADEAGALALAAAAMTESGISARVMSGGSTPSAARVQAGSMTELRP
                     GVYVFGDAQQLELGTIDWSQLALTVGSRVVSHARGRVIADAGSKALGADRAPWTSGYG
                     RVLDEPEARIVALSEHHATIEWPGALPALGSTIRIVPNHVCSAVNLAEEYVLPDGGRW
                     PVAARGRNH"
     gene            6046..6864
                     /locus_tag="PKJ61_02810"
     CDS             6046..6864
                     /locus_tag="PKJ61_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HOC12788.1"
                     /translation="MRIATVNVNGIRASTRRGFAEWLAARDCDLIALQEVRARADLVP
                     AEVVAGYHFAYHEGDRAGRNGVALLSRAPLLNVRHGFDHPSDSEGRYLEADVDLNGWR
                     LTVGSLYLPKGGRADDTPTELSRYRHKRRFMRSFRPYLTRARLRAAASGREFLVMGDF
                     NVAHTTLDVRNWRSNQKNVGFLPEEREWFGSLLGPRTLVDVVRRLHPDTYGPYSWWSW
                     LGNAFNNDAGWRIDYHLATPGLARAALVGGTDRAVSYEARLSDHAPVVVDYADQ"
     gene            complement(7028..7969)
                     /locus_tag="PKJ61_02815"
     CDS             complement(7028..7969)
                     /locus_tag="PKJ61_02815"
                     /inference="COORDINATES: protein motif:HMM:NF027439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003860 - 3-hydroxyisobutyryl-CoA
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HOC12789.1"
                     /translation="MSEAEVLVEVRAGVGRIRLNRPRQINALTVAMMRAIGDALELFA
                     ADAEVSSVELTGAGERGLCAGADVRALRELALSGDDVTPFFATEYGVNLQIASFPKPY
                     RAVMTGITMGGGMGLSVHGSQRVVDATSRLAMPETQIGLFPDVFVTPLLARMPGEIGT
                     HLALTGASVGAADALRLGLADVAAGSVGEPDAGLDAGWIADGYAGDDPAEIIGRLEQH
                     ADPAARAAAAELRGRSPISICVTLEALRRAARTVALGDQLPTELALAVRLATGHDFAE
                     GVRAQLVDRDRNPRWQHDSIEAVTRAEVLAYFEPLAG"
     gene            complement(7966..9408)
                     /gene="mptB"
                     /locus_tag="PKJ61_02820"
     CDS             complement(7966..9408)
                     /gene="mptB"
                     /locus_tag="PKJ61_02820"
                     /inference="COORDINATES: protein motif:HMM:NF038066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol phosphomannose-dependent alpha 1,6
                     mannosyltransferase MptB"
                     /protein_id="HOC12790.1"
                     /translation="MRGDIATAWTHRPVRRGLLGSVLLFLGSLTPAYLPQNSPWWEPM
                     RALGLDNWPARVLGTALVVAAVALLVEAWFKLRPSLHHEVKHWPITLLWSLPLLLAPP
                     IFSHDAYAYAAEGWLLRNGLNPYTNPISVLPGAFADQTAWLWRYTTAMYPPLSLEMFH
                     GLVVLAGNNPYFSAMAMRLPALLGVALIVYFLPRIAVHMGADVQLTVWFSAINPLLII
                     DFVGGAHNDALMMGLVVLAIWLAYERRFWWAAITVGVAACIKQPALLAFYPVALIRHP
                     WKTLSWPDTSAAMLRLVGSLGAAVAAFVAISLATGLGFGWVDAADVPGMIVTLAPFTL
                     VGEGLRLAFEFFGQPVAGQWIKDGLRYLGLLLTVVVIGWLGLGVGRRQPVKFLSWSYL
                     VFAFGGIALNSWYLTWGGLLLPLTKPTNRIIGTAVTLTTILLAYGAGNLAWRNGAVAL
                     GFAALALMLVLLYRHVQEHNERLESEGASA"
     gene            9817..11670
                     /locus_tag="PKJ61_02825"
     CDS             9817..11670
                     /locus_tag="PKJ61_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020393154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HOC12791.1"
                     /translation="MTEVTTLDHVVIRFAGDSGDGMQLTGDRFTHDTANAGNDFSTLP
                     NFPAEIRAPAGTVAGVSSFQLHFADFDIRTPGDRPDVLVAMNPAALKANLGDLAANGL
                     IIADTADFTARNLAKVGYGSDPLTDGTLHGYQVVALDLSGQASAAVAEFGLGRKDAVR
                     TKNMYALGLLDWLYSRPVEGTIAFLERRFATSPQIRDANIAALKAGYAFGETTEVFTN
                     RFAVAPAPAPLGTYRQISGNRALAFGLMAGAAKAGLQLFLGAYPITPASDVLHYLAAA
                     KDAGVMTFQAEDEIAAVGAALGASFAGQLGVTTTSGPGLALKMETIGLAVMLELPLVI
                     CDVQRAGPATGMPTKTEQADLTQALFGRHGEAPLPVIAAQSPGDCFAAAYEAVKIAID
                     YRTPVILLSDGYLANGAEPWLVPDLADLPEIDPKFAAEPNRPDGEFWPYLRDEKLARA
                     WAVPGTEGLEHRIGGIEKADGTGAISYDPANHEFMVNLRQRKVDGIQAVVPPVEVDDP
                     SGRAKVLVVGWGSTYGPISAAARRVRRTGRELACVQLRYLNPLPANLGEVLAAYDRVI
                     VPEMNLGQLAMVLRAKYLAPVVSYARVRGLPISLSELAKDLTEMIDEEAAK"
     gene            11676..12734
                     /locus_tag="PKJ61_02830"
     CDS             11676..12734
                     /locus_tag="PKJ61_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013069470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HOC12792.1"
                     /translation="MPSVPTPEATSGLAKVPLSISPLTRKDFTTDQEVRWCPGCGDYA
                     VLATFQGLMPTLGIKRENTVIVSGIGCSSRFPYYVDSYGMHSIHGRAPAIATGVAMSR
                     PDLAVWVVTGDGDALSIGGNHLIHALRRNVNITIMLFNNRIYGLTKGQYSPTSEQGKV
                     TKSTPAGSVDRPFNPVSLALGAEATFVARTVDSHRAHLSEVLTAAISHRGASFVEIYQ
                     NCPIFNDGAFDALKGADSGLIPLKHGEPIRFGPENSRGVVRDAGGHLQIANVAEVGLE
                     RVLVHDAHHDDPGQAFAISRLTDLAGLASSPIGIFRDVQRPAYDDLARDQLSVLVDPA
                     ARLAQLQDLITGADTWQV"
     gene            12822..13127
                     /locus_tag="PKJ61_02835"
     CDS             12822..13127
                     /locus_tag="PKJ61_02835"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOC12793.1"
                     /translation="MWSRSPAATNRRGERAYGFAHRAFEVPNRVDTRFALASGSKTFT
                     ALAVLRLVEQGLIALDAPVRPILSDDLPLVDAGVTIEQLLTSASPGNFAPDWNPRHR"
     gene            complement(13182..13382)
                     /locus_tag="PKJ61_02840"
     CDS             complement(13182..13382)
                     /locus_tag="PKJ61_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC12794.1"
                     /translation="MHTTHMVTFTQMAELGTLPVRVPAPAIPEARLVPSSRFSLREYS
                     MHTRRFAAHRWFRLAATEPQAC"
     gene            complement(13407..14471)
                     /locus_tag="PKJ61_02845"
     CDS             complement(13407..14471)
                     /locus_tag="PKJ61_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020391889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOC12795.1"
                     /translation="MGRTVIPVTMKTIATELGVSITTVSNAYSKPDRLSAELREQVFA
                     KAEALGYCGPSAAGRALRSGRTDVCGFYLGSHLAFAFSDPYTVILLSGIGETLEEFGS
                     SVLLLHASPDGESEVLQRAAVDAVVAAMPFEPHPGLGMLRRRGVPVVGTHRGSEGDWV
                     AINDAKAGRLVGKHLARLGHSRIVVIVPGTLPAGREVLDVELAPGGAWPVGFPGVRVG
                     YAAERVRGLQETLPGASIRLVCAGQNIKESGRRAGAHVLDDQHRPTAIVALSDVLAIG
                     VWDAVRERGLTPGRDVSITGFDDLPDAGFLGLTTVSQPIAEKGRLAARLAMDPDYPDR
                     QVLLPVELKVRASTGPAPLN"
     gene            14556..14801
                     /locus_tag="PKJ61_02850"
     CDS             14556..14801
                     /locus_tag="PKJ61_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC12796.1"
                     /translation="MSIAFTGLRIARWEGGLFVGYYLAYLVFIVLTAVEHDRLGSFSQ
                     DMAGFVLPLTVLTLIVLSHGEWRQRRARTSPPRPSSP"
     gene            14830..16749
                     /locus_tag="PKJ61_02855"
     CDS             14830..16749
                     /locus_tag="PKJ61_02855"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HOC12797.1"
                     /translation="MTDFDLDPAVRPQDDLFRHVNGRWLAATEIPADKHAAGAFMELR
                     DASEQAIKDIVTGLTGAEPGTEAHLVEHLYGSFMDTEEVDARGLAPLTPLLAEIDAIA
                     SIDDLLDYFGRSLRRGLAAPIGVEVEADPGEPSRYALFVGQSGISLPDEEYYRLPEHA
                     EILSSYRDHVNRMLMLAGISASVAGITVDLETDVAAKHWDKVRTRDMVAMYNPTDWAD
                     LDAAGVPWTRVLLAAGVPQVEQVIVAQPSFLTDLAALLTPGRLDDWKQWCRWTLVSGL
                     APFLPEALVQERFNFYGTTLQGVPQLKERWKRGIDLVERSLGEAVGKLYVERHFPPAA
                     KERMDELVANLLAAYRESITKLDWMTDATRAEALLKLANFTPKIGYPTVWLDYSGLAI
                     DPRDLVANVLACAEFHTTDELSKLGGPIREWEWLMTPQTVNAYYHPLRNEIVFPAAIL
                     QPPFFDAEADDAINYGGIGAVIGHEIGHGFDDQGSTCDGEGRLRDWWTPADREAFTER
                     TKALIGQYSALAPAQLPELHVNGDLTIGENIGDLGGAAIAYEAWQLALGGRAAEPVDG
                     IPAEQRFFLSWARVWQSKRRDEALRQQIATDPHSPEEFRCNQVVRNLPAFYAAFDVTE
                     ADAAWLDPSDRVKIW"
     gene            16746..18053
                     /gene="nhaA"
                     /locus_tag="PKJ61_02860"
     CDS             16746..18053
                     /gene="nhaA"
                     /locus_tag="PKJ61_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HOC12798.1"
                     /translation="MTQTPVTLARGAYAELQRINRILRRESVGGILLLVAALAAMLCA
                     NFAPGFYFGLRDLHLGGDLLGLHLDLSIGHWAADGLLAVFFFLVGLELKREFVTGDLR
                     DPKTALVPVVAAVGGVAVPALIYSAFTFAYPDMLAGWAIPAATDIAFALAVLAVIGSH
                     LPAALRTFLLTLAVADDLIAITIIAFFYTRDLKLEYFVAGLVPIVAFGLLAHLAQRFL
                     VRHGWAAFLLLLPIGLVAWALAYNSGVHATVAGVLLALGVPVRPAKGDTAEHGLAEVL
                     EHKVRPFSAGVAVPVFAFFSAGVLVGGLDGFVAAVTSPIGLGVIVGLVVGKAIGISGA
                     TWLVTRLRHAQLDPDLAWIDVFGLAVLGGIGFTVSLLVSELSFGQGTPHDDIGKVAIL
                     TASVLAATLAAVLLGSRNRHYRAVEVAEQVDADADGVPDRFQH"
     gene            complement(18481..19911)
                     /locus_tag="PKJ61_02865"
     CDS             complement(18481..19911)
                     /locus_tag="PKJ61_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017934090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HOC12799.1"
                     /translation="MTELTELDALAVSRGVRRGDFAAVEVTQAHLARADRLSDTVGAF
                     ARLTPELALAQAAAVDARVRSGEADGALLGVPCPIKDLSPVAGVGFEAGSAAMAGYVA
                     EVDDDIVSWFREAGTVLLGKTATPEFGLPCYTEPETGPAARTPWDVRRSAGGSSGGAA
                     AAVATGLAPIAHASDAGGSIRIPASACGLVGLKASRGRVSTGRFREPGPGLGTDGVLS
                     RTVADTAAALDVLAGHGIGEPYVAAAPSGRFLDAVTRSPGSLRIGVLVEPAIAVAEVH
                     PACLDAVDRTAATMLELGHEVVAAPQPFTVDRWEAFSELWAVGAAMVPLPAEAEAELR
                     PLTRWLRERGRQATGLEYARAAAAVQRIGVDIARAWAGFDVILTPTLAQPPALVGSLR
                     NDADPAEDFAAQTRFTPWTSVYNLSGGPAISLPLHTAEVDGAVVPIGVMFGAAHGAEE
                     LLLSLAGQLEAAVGWEHPFLVSDPKG"
     gene            complement(19908..20348)
                     /locus_tag="PKJ61_02870"
     CDS             complement(19908..20348)
                     /locus_tag="PKJ61_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC12800.1"
                     /translation="MFVLGDMIALDNLPGVAQVAIQGARYAASKIIGEQTGHPVTADF
                     KYHDKGSMAVISRFGAVAKVGRLELTGFPAGFAWLFVHLIYVVGFKNRLTTLLHWAIT
                     FIGRGRCERVITEQQMVGRLAVLQRGDDFQPTITGTLAPDKIKP"
     gene            complement(20369..20827)
                     /locus_tag="PKJ61_02875"
     CDS             complement(20369..20827)
                     /locus_tag="PKJ61_02875"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOC12801.1"
                     /translation="MAELTESEAERRSLTTFVIVGAGPTGVEMAGQIAELTRQTLRRS
                     FRRVDTTKSRILVLDGASAVLAPFGSKLSVAARRELERNGVEVRLGALVADVDANGLE
                     VRYQDGRTERIEASCKVWAAGVQASPLGRTLAEQSGAGVDRAGRVQVNPD"
     gene            complement(20874..21968)
                     /locus_tag="PKJ61_02880"
     CDS             complement(20874..21968)
                     /locus_tag="PKJ61_02880"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOC12802.1"
                     /translation="MSHPLVIVGYGTMGDYHRRNLATIDGFEVVGVVDIDEQARAAAQ
                     DEGVHAYASLPEAIAELEFDFVMIATPNDSHHDLAMTALAAGKNVLCEKPAMVTSLEL
                     VEVIAAAEQAGKLFMVHQNRRWDPDYLSMKAVYDEATIGPVTYVETRVHGSRGIPSDW
                     RTKKENGGGMLLDWGVHLVDRLLLMVDAPVTDVYANLSYALGQEVDDGFKAYLTFGNG
                     VTALVDVSTTSFQPMPLWSLQANRGSARIENWATPGRILRLGVVEEDDAAPIQAGAGM
                     TKTMAPRVKDYSNSYKLDESLEDLPVPVVEADVRDFYRNALAVVDAGAEPVITNAQVL
                     RTMRLLEACVQSHELREVVHFEPAASLQTA"
     gene            complement(22070..23089)
                     /locus_tag="PKJ61_02885"
     CDS             complement(22070..23089)
                     /locus_tag="PKJ61_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012921862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOC12803.1"
                     /translation="MSRPVTLFTGQWADLPFEEVCRLASGWGYDGLEIACWGDHLDVY
                     RAASDDAYVAEKKATLAKYGLGVWAISNHLKGQAVCDDPIDFRHRDMVSDAVWGDGDP
                     EGVRQRAAEEMKLTAIAARKLGVDTVVGFTGSKIWQYVAMFPPVPDSVIEDGYADFAR
                     RWNPILDVFDSEGVRFAHEVHPSEIAYDYWSTKLTLEAIGHREAFGLNWDPSHMMWQE
                     IDPVGFLVDFADRVYHVDCKDTKMACGNGRNGRMGSHLPWGDPRRGWTFVSTGNGDVP
                     WQRCFRTLNAIGYTGPISIEWEDAGMDRLRGGKEAIDFVRANLFDAPTASFDAAFSTQ
                     VQPGE"
     gene            complement(23106..24185)
                     /locus_tag="PKJ61_02890"
     CDS             complement(23106..24185)
                     /locus_tag="PKJ61_02890"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOC12804.1"
                     /translation="MSKYRVGIIGVGFIADYKHLEGLAANADLCDVVAFCDLQLDRAE
                     KGKARCGTPDAYVTTDWREVVADESIDVIYVCTWNISHAEITCAALEAGKDVMCEKPM
                     AVTGEDARRMVETARRTGRKLTIGYQNRLREDAQFLRDVVDEGELGEIYVAKAHAVRR
                     RGVPTWGVFTDKEKQGGGPLIDIGTHALDLALWYMGNYEVDSVTGSVFEKLRDKPEGN
                     PGGGWDPETFSVEDSAFGYIRMKNGATIFLEAAWALNVKDPREAAVTLIGTDGGAEIE
                     HHGKGYEATVNKVLANRMIVSEPTPSSPGSEDFARLGAAETRQWLEALRNGTDPVVLP
                     DEACRVTEILEAIYASSASGEPVKF"
     gene            complement(24535..25464)
                     /locus_tag="PKJ61_02895"
     CDS             complement(24535..25464)
                     /locus_tag="PKJ61_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020391941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOC12805.1"
                     /translation="MLERIEQRLADAAIAQTDFVTEAAQHIISAGGKRFRPLLVVLAA
                     LLRADADDELIIRAALVMELTHVASLYHDDVMDEAELRRGAPSANQRWDNTVAILVGD
                     FLFARASALVATLGTEYVKLQADTFARLVQGQIAETRGPLDGDDRLEHYLDVVSDKTG
                     SLIAASTTFGGMVAAVSPDELAALAAYGEEIGVVFQLSDDIIDIASDTSGKTPGTDLR
                     AGVPTLPTLLARRSAEPSDARLLALLDADLSADADLAEALGLMRAHPAMAEAREVIHT
                     RAEIARGHLAPLPEGPVKQALSSLCDGVISRSS"
     gene            complement(25559..27121)
                     /gene="nuoN"
                     /locus_tag="PKJ61_02900"
     CDS             complement(25559..27121)
                     /gene="nuoN"
                     /locus_tag="PKJ61_02900"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020391940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HOC12806.1"
                     /translation="MKSIAAPTFEWLQLAPVLTILLAACLGIVIEAVLPRRLRFLAQI
                     GLFEVAIGVAFAFLVISWRQPEHSQGLLAMGSISLDGPTYLMWGALLVFGLLALLVFS
                     ERDLDNGATAFAASAASVPGSPAEAEANSARHEHSEVFPLAMFSLAGMLVFVAANDLI
                     TAFVALEVMSLPLYLLSGMARKRRLLSQEAALKYFLLGALSSAFFLFGMALVYGFSGS
                     FQLSAINDAIANPVYGRGLLFTGIALLAIGLLFKIGAVPFHNWVPDVYVGAPTPVTSF
                     MAICTKLAAIGATLRVFFVAFGAERWTWQPLLATVAVLTMGIGVVVALTQTDMKRLLA
                     YSSISHAGFILVAVVGATAGVTGQVNSFGSILFYLVAYGFATIAAFAIVTMVRDSAGE
                     VHAISGWAGLGRKHPWLAGIFALLMLSFAGIPLTGGFIGKWAVFSAAWRGGYSWLVVA
                     AVAFSLVAAYVYLRVIVTMFFAEPALGVGVGKASGMTWVPVVLGAVASAYLGLFPGPL
                     LDLVTSASTFLR"
     gene            complement(27118..28629)
                     /locus_tag="PKJ61_02905"
     CDS             complement(27118..28629)
                     /locus_tag="PKJ61_02905"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016668043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HOC12807.1"
                     /translation="MTFPWLTFLGLAPLLGAAVLCLPLKHAARWVGMAFALITAVIGL
                     VIGGLYLAGQTFVERAPWIPAFGAGWAVGLDGMALVMVLLTVLLVPVVLLAEWRVGGG
                     GRWSPQVFFALVLALESLSIFTFMAEDVLLFYLFFEATLIPMYFLIGGFGGPKRAYAA
                     VKFLLFSLAGGLIMLASVIGIYAWSVQATGAPTYLLSELQQLQIDPMLGRWLMLGFLV
                     AFIVKAPMVPVHTWLPTATEQATPGSSVLLVGILDKIGTFGMIRFCLGLFPEASTWVA
                     PVMIGLALVSIIYGAIAAIGSSDLLRLVAYTSISHFGFMVLGIYTFTSVGISGSIFYM
                     VNHGFSTAALFLVIGFLISRRGSAAVADFGGVQKLAPVLAGVFLMAGLSGLALPGMSS
                     FVSEFMVLAGAWSAIPWVGAVASLGTVLAAVYILLMYQRTMTGPVTPAVAEHFTRDAT
                     ASEKWVIGPLLAVILLLGFLPNIALNVIEPTAHQTMSIVGIADPVAPLGGEAK"
     gene            complement(28645..30552)
                     /gene="nuoL"
                     /locus_tag="PKJ61_02910"
     CDS             complement(28645..30552)
                     /gene="nuoL"
                     /locus_tag="PKJ61_02910"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HOC12808.1"
                     /translation="MITLETLAPVAATGMFAYAWLMIAVPAVSAALLLLAGSILDRIG
                     HWIAVAAVTFSFSIAVCLFSQQLLSPEDARAVAVPMFEWITLGSWQVSAGLLIDQLSI
                     LFALLITGVGALIHLYSVGYMAHDERRRRFFAYLNLFIAAMLVLVLADNYLVLFVGWE
                     GVGLASYLLIGFWQHKPSAAAAAKKAFVMNRVGDLGMTMAVMSMLALFGSSAFVAVNA
                     NAEHLDPFWATTIGLLLLLGACGKSAQVPLQAWLLDAMEGPTPVSALIHAATMVTAGV
                     YLVVRSHAIFEMSSAASTAVVVVGTVTLLAGAWIGTSKDDIKKVLAGSTMSQIGYMML
                     AAGIGPAGYAFAIFHLLTHGFFKANMFLGAGSVMHAMNDDVDMRHYGALAKVVKVTFW
                     TFAMGYLAIIGFPGWAGYFSKDHIIEAAFEHSPIAGTAALIGAGITAFYMTRLMLFTF
                     FGEKRWQPGVHPHESPRTMTVPLIVLATLSVVAGALMNGWIQHWLEPATGAHAHEVSL
                     IPTPIGWLTLLLVAAGVALAWWLFGTKPIPAVAPASTNPFTVMGRNDLFGDAVNNVLV
                     VEPTMVAAHGLVANDDRIIDVGADKVADMFNGLSTQTRKLQTGQVRTYALTMTIGAVL
                     VGVVMILSQLG"
     gene            complement(30560..30859)
                     /gene="nuoK"
                     /locus_tag="PKJ61_02915"
     CDS             complement(30560..30859)
                     /gene="nuoK"
                     /locus_tag="PKJ61_02915"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002515419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HOC12809.1"
                     /translation="MSPDNYLYLAAILFTIGAVGVMVRRNALVAFMSVELMLNAANLA
                     MVAFAMARGGLDGQVATFFVMVVAAAEVVVGLSIIITIYRTRRSTSVDAASLLKL"
     gene            complement(30859..31665)
                     /locus_tag="PKJ61_02920"
     CDS             complement(30859..31665)
                     /locus_tag="PKJ61_02920"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002514926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HOC12810.1"
                     /translation="MIPLVTGAEVTFWIVAPLMVLGSLGLLVFRKAVYAALSMAFVMV
                     NLAVLYASMDAMFLTFVQIIVYTGAIMMLFLFVLMLVGIGHQADSLVETIPGQRWLAG
                     LAGLGLLGLILFGIGGSLVGGPRIGLDAANAAHGGNVESLAALIFGRYVFVFELTSAL
                     LITAAVGAMVLGQHFRVRPRATQPELAEKRMRDYRALGTHPGPLPNSGVLANHNSIAT
                     PALLPDGTVSEASISPTLAGRGVIVDAPAASLATARAFESIESHQGEEDE"
     gene            complement(31662..32225)
                     /gene="nuoI"
                     /locus_tag="PKJ61_02925"
     CDS             complement(31662..32225)
                     /gene="nuoI"
                     /locus_tag="PKJ61_02925"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014847367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HOC12811.1"
                     /translation="MGIFDPWAGFGVTFRTIFRKTFTQGYPEKGKEKITAPRFHGRHQ
                     LNRWPDGLEKCVGCELCAWACPADAIYVEGADNTEEERYSPGERYGRVYQINYLRCIL
                     CGLCIEACPTRALTMTNDFKLADTTREKMIYTKDRLLAPLLPGMEEPPHPRRLGADEK
                     DYFLGLPATGKPDNRTPVVTDTPGGTR"
     gene            complement(32225..33514)
                     /gene="nuoH"
                     /locus_tag="PKJ61_02930"
     CDS             complement(32225..33514)
                     /gene="nuoH"
                     /locus_tag="PKJ61_02930"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007076569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HOC12812.1"
                     /translation="MNDPWWLVLIKVVVLFVVLLLWTIFNVWYERRLVGKMQHRLGPI
                     MNGPFGLGQALADGMKLLIKEDFMPSMVDRALFVIAPLIAGTAAFTAWAVIPFGGEVE
                     IFGVKTLLQLTDLPVGVLVLLAVTSVGIYGFVLAGWSSNSPYALLGGLRSSAQMISYE
                     IAMGLSLVSVFLYAGSMSTHQIVEAQRAPLGPEWVGLQSWYWLLLLPSFVIYLISMVG
                     ETNRAPFDLPEAESELVSGYITEYSGFKYAVYFLAEYINMATVSAIATTLFVGGYLPF
                     WPLNLIPVLNNPWLGPIWFAIKVQLFISLFVWLRGTLPRFRYDQFMKLGWKALIPISL
                     VWIIIIATFQSLRQENLLQGPVLWLAIGIILALLVAFMLWSPSAEPEPEPASQGEFDA
                     FAGGYPVPPMPGQVLPELQDVVASTAEAAPQPAGEEQ"
     gene            complement(33511..35958)
                     /locus_tag="PKJ61_02935"
     CDS             complement(33511..35958)
                     /locus_tag="PKJ61_02935"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016456807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit G"
                     /protein_id="HOC12813.1"
                     /translation="MSVVTKPDQVAPVETITVTIDGTEVQVPKGTLAIRAAEMIGIAI
                     PRFCDHPLLDPVGACRQCLVEVPDMGNGRGMPKPQASCTLECAPGMQIKTQLSSPVAE
                     KAQKGMLEFLLINHPLDCPICDKGGECPLQNQALSNGRGESRYEGVKRTFPKPVPISA
                     QILLDRERCVLCARCTRFSEQISGDPFIALVERGALQQVGKYAKEPYDSYYSGNVVQI
                     CPVGALTSAAYRFQARPFDLVSTPSTCEHCAGGCQLRVDHRHFQVRRRLAGDAPEINE
                     EWNCDKGRFAFVSARGDDRLRSPLIREGDELRVASWPEAIDAAVAGLTAAGSSVGVLT
                     GGRLTLEDAYGYSKFARTVLGTNSIDFRSRPIGAGEAEFLAAQVAGRALGDGVTYSEL
                     EAAGRVVLVGFEPEDESPLIFLRLRKGVRKSGLKVTAVAPYASTGSAKLNAELVVTAP
                     GEEAAALRGLTLDAGNVILVGERAALAPDALAAAAEVAATSGARLAWVPRRAGDRGAV
                     EAGCLPGLLPGGRPVDDASARVDVAAAWGSASLPTETGLDAPAMLAAAASGELKALLV
                     SGLEPVDFADAAAARAGLEAAGFVVALENRLSEVTERADVVFPVALIEERPGTFLNWE
                     HRPGRVNTVIKGQTPPMTDLRVLAALADALGRPMGIRTPSAALAELTELGAWTPMVDA
                     KPARTKSKTKKVPTSPTADTLPELDAGSVAAKLATWRQLIDGGRGQDGEAALAATARP
                     TVARVGSELAARLGVADGDEVTVGGATGWYSLPVQITPDLAPGTVWVPTNSPGTPLGE
                     LGLVHGDVVAISAGGWS"
     gene            complement(35955..37295)
                     /gene="nuoF"
                     /locus_tag="PKJ61_02940"
     CDS             complement(35955..37295)
                     /gene="nuoF"
                     /locus_tag="PKJ61_02940"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013160443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HOC12814.1"
                     /translation="MSDLLTPVLSANWGTDKSWKLAAYRDAGGYAGLPKALKLTPDEV
                     TNLVKDSGLRGRGGAGFPTGMKWSFVPKDNPNPKYLVVNADESEPGTCKDMPLLLASP
                     HTLVEGMIIASYAINCHTAFVYIRGEVLHVIRRMQAAVAEAYEAGYLGKNILGSGYDL
                     DIIVHAGAGAYICGEETALLDSLEGRRGQPRLRPPFPAVAGLYASPTVINNVESIASV
                     PAIVANGAAWFASMGTEKSKGMTIYSLSGHVNRPGQFEAPLGITLRQVLELSGGIRAG
                     HELKFWTPGGSSTPILTPEHLDVPLDYEGMGSVGSMLGTKALQVFDETTSVVRTTLRW
                     VEFYKHESCGKCTPCREGNWWLVQLLKKFESGTAVSGDVEKLLDICDDIGGKSFCALA
                     DGAVACVTSAVRHFRGEFEAGYSTPAWELFPYGRSALFEVPDRELATSTKGAHA"
     gene            complement(37295..38011)
                     /gene="nuoE"
                     /locus_tag="PKJ61_02945"
     CDS             complement(37295..38011)
                     /gene="nuoE"
                     /locus_tag="PKJ61_02945"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HOC12815.1"
                     /translation="MSGHEFQSFFPESGSVDLTDTSTNIDETTMAELRELAARYPQPR
                     SALLPMLHLVQSVDGRVSPRGIEACAEVLGITTAQVSGVATFYTMYRRRPAGEHHVGV
                     CTTALCAVMGGDALLEKVSEKLGIEPDETTPDGKFSLERLECNAACDFAPVMMVNWEF
                     MDNMDPDKAEHLLEDLAAGKEVKASRGATLTSWREAERVLAGFPDGRADEGPSAGEAS
                     LLGKKIAEANSWSAPAAEEA"
     gene            complement(38008..>39104)
                     /locus_tag="PKJ61_02950"
     CDS             complement(38008..>39104)
                     /locus_tag="PKJ61_02950"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002515416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HOC12816.1"
                     /translation="EKNMEYRSWTQGTTFVTRMDYVAPIFNEVAYCLAVERLLGIEDQ
                     IPERASDIRVLMMELNRIASHLVAIGTGGMEIGALTVMTIGFREREMILDFFEAVTGL
                     RMNHAYVRPGGVANDLPETGIAQLRDVIAWLDKHIPEYAAFCNENPIFKGRMCGIGVQ
                     DLSACFALGVTGPVLRSTGYAWDLRKQQPYCGYENYDFDVITWDTSDAYGRFRVRLDE
                     MWESLKIVKQATDRLEASTGQPVMIDDPKIAWPATLAVGPDGQGNSHEHIKHIMGESM
                     EALIHHFKLVTEGFRVPAGQAYVPIESPKGEIACHVVSDGGTKPFRCHLRDPGFNHLQ
                     SVPIQCEGGMLADVVVAIGSLDPVMGGVDR"
BASE COUNT         6651 a        13578 c        12980 g         5895 t
ORIGIN      
        1 gaacccgcga cacagcgatt atgagccgct tgctctaccg cctgagctat cccgcccccg
       61 ttcggatcca gcttcgctgg gacgcgggcg gtaactccgc aatccgaaca cagagccccc
      121 atgcggaatc gaaccgcaga ccttctcctt accatggaga cgctctgccg actgagctat
      181 aggggccgga cagcggttga cactacccca gtttgggcgt tcgccccaaa tcgagcgttg
      241 ggacgcggtt cggccgcctc gatcagtcga gttcggcgcg gtagcgggac aggtctacgc
      301 gtcctgcgcg gacgctgacg ccctccccga tgagccgctc ggacgcctcc gcgaccaggg
      361 gcgcggcgat ggttccgtcc gctcggatca cccgccacca gcacgtctcc ccgccgctgc
      421 cggcgaggac ccgggccacc aacctcggcc cgcagccgac cacccgggcg atgtcgccgt
      481 aagtgctcac ccggccggag gggatcgcgt ccgccaccag caggatgctg tcggcgagct
      541 cggccatgct gggcatgact gcactctacg aggccgcggg cggcgggcct atcgtggaac
      601 cgagtgagag gagcaccatg accgagtacc gcatcgaaca cgacaccatg ggcgaggtcc
      661 gagttccggc gagcgctctg tacgcagccc agacgcagcg cgccgtcgag aatttcccga
      721 tttcgggacg cggcctgtcc agccaccaca tcgccgcgct cgcccagatc aaacgcgcgg
      781 ccgctctggc caacgccgag ctgggcgtgc tgtcaccgga tgtggcggac gcgatcgtgg
      841 ccgcgtccga ggaggtgatc gccggtcagc acgacgacca gttcccgatc gacgtcttcc
      901 agaccggctc aggcacctcg tccaatatga acgccaacga ggtggtggcc aatctggcct
      961 cgcgagggct cggccgtccg gtgcacccga atgaccaggt gaacgcgtcc cagtcgtcga
     1021 acgacgtgtt cccttcgtcg atccacatcg cggccgtgca ggctatccac gagacgctgt
     1081 tcccagggct ggagacgctg gccgcgtccc tggaagcgaa gtcggccgaa tacgcgagcg
     1141 tggtgaaatc cggtcgcacc cacctgatgg atgcgacccc gatcacgctc ggccaggagt
     1201 tcagcggcta cgccacccag gtgcgctacg gcatcgagcg ggtgcgcgcg gcgctccccc
     1261 ggctgtgcga acttccgctg ggcggcactg cggtcggcac cggcatcaac accccggacg
     1321 ggttctccgc gcgggtgatc gaactgattg cggcgaacct ctcgctgccg ttcgtggagg
     1381 cgccaaacca cttcgaggcc caggccgccc aggactcgct ggtggagaca tccggggcgc
     1441 tgcggacgat cgcggtgagc ctgaccaaga tcgccaacga cctgcgctgg atgggttcag
     1501 gaccacgcac ggggatcggc gagatctcgc tgcccgacct gcagccgggc tcctcgatca
     1561 tgcccggcaa ggtgaaccca gtgctgccgg aggcgacggt gatggtggcc gcgcaggtga
     1621 tcgggaacga cgccgcgatc gcgttcgccg gtgcgcaggg caacttcgac ctgctggtga
     1681 tgctgccggt gatggcccgc aacctgctgg agtcgatcac cctgatcggc aacgtctcgc
     1741 gagtgctggc cacccgctgc gtggacggga tcgaggccaa cgtggagcgg tgccggcagt
     1801 tggccgagtc gtcgccgtcc atcgtgaccc cgctgaaccg gatcgtcggc tacgaggcgg
     1861 cggcgaagat cgccaagcat tcggtgaagg cgaacttgac cgtggcggac gccgtccgcg
     1921 acctcggcta cgtcgagcgc ggcgaaatca ccgaggagca ggtggccacg gcgctggacg
     1981 tgaccacgat gatcggggtc aatcgctgat cgccagccgg gcgccgaggg cgatcatcac
     2041 gccgccgccg gttgcgctca gccgacgcag ccggcgcggc gaggacgcga accagctgcg
     2101 tgccgtgccg gcgaacagcc cccacagcga gtcgcagagc gctgcgatcg cgacgaagac
     2161 cgcaccgagt agcagcaatt gcgggagggc cggctgcgcc ggatcgatga actgcggcag
     2221 catcgcggcg aagaagaccg cggtcttcgg gttggtgagt ccgactacga agccttcggc
     2281 gagcacccga cggttggacg gagccgcgtc cggcacggca agttcacgcc ggtgtcggat
     2341 cgcctgcaca ccgaggtaga tcaggtaggc cgcgccggcc agccggatga tctcgtagct
     2401 gatggtcgcg gctgcgatca gcgcaccgag gccgaaggcg acggccgcga ccagcaccag
     2461 tgtgccgatg gcgttcccga gggcggtgcg gacggccgcg ccgcgaccca tcgcaactgc
     2521 gcggctgacg acgaacagca cgctcggccc gggaatcagg atcagcacca cggccacggc
     2581 gaggaacgcc agcagttgcg aggtcggcgg catggccgaa gcgtagtcgt gcccggtagc
     2641 ggctacgaga tcacatccac cggaccgcag cgatagagct tcggcacggc cccgccgttg
     2701 gtgcgataac agacgagttc tcatcttccc aggagagaac tgagcttcgg cgagcgactc
     2761 aggactcgtc gcgcagccgc acgcgtgagc cagcgtgagc tggctcgaac ctcaggcgtc
     2821 aagcaaccgc tgatcgccgc catcgaaacc ggagcccgcc agccaagcga ggccgtccga
     2881 acggctctcg aagaagcgat ccaggtgcgc ccatcccgac tgctcgaact ggcgcgcgat
     2941 caggtgctag ctgctgtgca ggcgatcggc ggcgaggacg tccgggcctt cggctccgtc
     3001 gcccgcaggt cgacgtggtc tccacgggca gttcaggccc ggtgttgacc cgggcactcg
     3061 cggaagcggt ggccctgtga ccgacgaccg ggttgctcgc cggcttgaag acctggctca
     3121 gatcggcgcc gaggccgcct acctggtcgc caaaggtgcg gacgcctatc tggccgacac
     3181 gatggacggt gcgctcctcc aagacgccgg cgaacgaatc ctgatcaaga tcgcgactgt
     3241 cgccgagaag ctgccaaacg acttcaaggc agcccatccg gacatcgact gggccggcat
     3301 caaccggatg cgcaatctgg tcgcacacca ctgcggcaag gtcaatgacg acttgatgtg
     3361 gcaggcgctc accggccgta tcccgaatct tgtgaaccgc atcgtcgcct agcccgccgc
     3421 gtcggtcacg gccggtgtgg caatgcacca aaacggcccc tgaccagggg tttcacctga
     3481 tcaggggccg atgcgtggca ggtgtagggt tcgaacctac gtaggcttag ccgacggttt
     3541 tacagaccgc tccctttggc cactcgggca acctgccgtg cgtcggacac tgtagcaaaa
     3601 cgcgcgagcg gtcacgaatc cgcctcattg ggccaaaaca cgtccgatcg gacgcgaccc
     3661 gcggcccatc gccagatgga ctcacagcgg acgcgattcg cgccggcgaa gctcccgacg
     3721 cagccgtcgg atggtcttga tcccgaccac ggccgctgcc tcagcccgcc cacggtgacc
     3781 cggcgtgccc ggtttccccc acgtccgccg aaccattcga gcacttcggc caggtcttcg
     3841 tcgccggccg gttgcgcaat cagcaaacga cccagcgcca cccccgtcgg tggcggcgag
     3901 cgccacggct gcgaccagcg gcttggcgaa ccgcacaccg tcccgttcat cgacgaactc
     3961 gggaggaacg tcggcggcgt accaggcgct gccgtcacgc ggcggtttcg gcgctccggc
     4021 gagggtgatc gtgtcgcgag cgcggaacgg cgccaggccg ccgccggccg catccagagc
     4081 ggatcttccc caccggcagg cgtgcggcgc gcacgcgcac gccgcctgcc aacgcaccca
     4141 gcggaccgtc acgtcaacag gccgaacgta ggttccggga agcacccgaa ccagctcgcc
     4201 gacgcgccag ccgactgagc gtcgaccgcc gcaggtcagc cgcccggaag atgcgctggt
     4261 cgggccgtct ctccagaaac aaagcggtca caatggcatc ttcggtaagc cgagcagatt
     4321 cccgtcagcg ggtcgacatc gaccctgtgg atggcgcgca acgcgtccag gtgctgtgga
     4381 aaacccgact cgccgcggat cccgtccgat ccgtagcgaa gcgtcccgcc gattgcgggc
     4441 gtgaccgcag atctggtgac aatggacgca agcacaccag gaggacacgt ggcagcagag
     4501 aactcgttcg acatcgtcag caaggtcgat caccaggagg tggacaacgc cctcaacatg
     4561 gccgccaagg aggtgcgcaa ccgcttcgac ttcaagggca cggacgcgtc gatccgctgg
     4621 tcgggcgaag cgatcgagat ggaggccagc ggcgaggagc gggtgaaggc catccttgac
     4681 gttttccaga ccatgctggt gcggcgcaag atcgacctga agtcgctgga cgccggcgag
     4741 ccgcggctct cgggcaagat gtggaagctc accgcctccg tgaagcaggg catcagccgg
     4801 gagaacgcgg ccaagatcgc caagctgatc cgcgacgagg gccccaaggg cgtgcgcacc
     4861 caggtgcagg gcgaggaact gcgggtcttc tccaagaagc gcgacgacct gcaggtcgtg
     4921 cagcaactgg tccgcggcgc cgattacgac ttcgcagtcc aattcgacaa ctaccgatag
     4981 tcgatggccc ggtttccgag ggcaggccgt tgacgtgggc gtcactccct acctgaggct
     5041 cgacctggac gtcctcgaac gcaacctgga cgcgatggcc gcgcacgccc gcacgctcgg
     5101 cctggcgctg cggccgcacg ccaagaccca caaatgcccg cagatcgccg acatgcaact
     5161 ggcccgcggg gcggtcggcc tgaccgtcgc gaccatcggc gaagccgagg ttttcgctgc
     5221 cgccggcgca ggcgatctgt tcatcgccta cccgctctgg gtggacgcgg acgacgccga
     5281 ccggctgcat cgcctggctg agggcggcgt ccggctgagg gtgggctgcg actcggccga
     5341 ggccggcgcc caactgcgtg gcctgccggt cgagctggtg gtcgagatcg actccggcca
     5401 ccaccgcagc ggggttgcac ccgcagcagc gggcacgctc gcgtccgggc ttgccgacct
     5461 aggaatggcc gtcgccgggg tgttcacctt cccaggacac agctactcgc cggacgaggc
     5521 gtcggctgcc gctgcggacg aggccggcgc gttggccctg gcggccgccg caatgaccga
     5581 gtccggaatc tcggcgcggg tcatgagtgg cggctccacc ccgtccgctg cacgcgtgca
     5641 ggccggttcg atgaccgaac tgcgtccggg tgtctacgtc ttcggcgacg cccagcagct
     5701 cgaactcggc acgatcgact ggtcgcagct cgcgctgacc gtcgggtccc gcgtcgtttc
     5761 gcacgcccgt ggacgcgtga tcgcagacgc cggcagcaag gcgctcggcg ccgaccgggc
     5821 accctggacg tccggctacg gccgggtcct ggacgagccc gaagcgcgca tcgttgccct
     5881 ttccgagcac cacgccacca ttgagtggcc gggagccctg cccgcgctgg gcagcacgat
     5941 ccggatcgtg ccgaaccacg tctgtagcgc ggtcaacctc gccgaggagt acgtgctgcc
     6001 cgacggtgga cgctggccag tggccgctcg cggacgcaac cactgatgcg gatcgccacc
     6061 gtcaacgtca acggcattcg cgcatccacc cgccgcggtt tcgccgaatg gttggctgcc
     6121 cgcgactgcg atctgatcgc cctgcaggag gtgcgcgccc gggccgacct ggtgcccgcc
     6181 gaggtggtgg ccggctacca cttcgcctat cacgagggcg atcgcgccgg acgcaatggc
     6241 gtggcattgc tcagccgtgc gccactgctg aacgtacgtc acggtttcga tcatccgtcc
     6301 gacagcgaag ggcgctacct cgaagcggac gtggacctca acggctggcg cttgaccgtc
     6361 ggctcgctct acctgcccaa gggcggacgc gccgacgaca cccccaccga gctgtccaga
     6421 taccggcaca agcggcgatt catgcgttcg ttccgtccgt atctcaccag ggcgcggctg
     6481 cgggctgccg cgtccggacg ggagttcctg gtgatgggcg acttcaacgt cgcgcacacc
     6541 acactcgacg tccggaattg gcgctcgaat cagaagaacg tcggtttcct gcccgaggag
     6601 cgggagtggt tcggctcgct gctcggcccg cgaacgctcg tggatgtggt gcgccgattg
     6661 catccggaca cctacggccc ctactcctgg tggagctggc tcggtaacgc cttcaacaac
     6721 gacgccggct ggcggatcga ctaccacctg gcgacgccgg gtctagcgcg ggcggcgctg
     6781 gtcggcggaa ccgatcgcgc agtcagctac gaggcccggc tcagcgacca cgcgcccgtg
     6841 gtggtcgact acgccgacca gtagtcggtt gatgggttct ccaggtctcg acccttcgac
     6901 aagctcgggg caggccaagc tcgaccagcc gaaaggttgc ccacgtcatc ccggcgaagg
     6961 acgggatctc ggttccgatt ggcgaagact ggccactttc gcgcccaagc gtagaggccg
     7021 attggtctca acccgccagg ggctcgaagt aggcgagcac ctcggctcgg gtgacagctt
     7081 cgatcgagtc gtgttgccat cgggggttgc ggtcgcggtc cacgagctgg gcgcggacgc
     7141 cctcggcaaa atcgtggccg gtggccaggc ggaccgcgag cgcgagttcg gtcggcagct
     7201 ggtcgccgag tgcaaccgtg cgggcggccc gccgcagcgc ctccagggtc acgcagatcg
     7261 agatcgggct gcgtccccgc agctccgcgg cagccgctcg ggcggccgga tcggcgtgct
     7321 gctccaggcg tccgatgatc tcggccggat cgtcaccggc atagccgtcg gcgatccagc
     7381 cggcgtccag accggcgtcc ggctcaccca ctgagccggc tgccacgtcg gcgagaccca
     7441 gccggagcgc gtccgccgca ccaacggacg caccggtcag cgccaggtgg gtgccgatct
     7501 cgccgggcat ccgggccaac agcggcgtca cgaagacgtc cgggaacaat ccgatctggg
     7561 tctccggcat tgccaggcga ctggtcgcgt ccaccacccg ctgggagccg tgcacgctca
     7621 accccatgcc gccgcccatg gtgatgccgg tcatgaccgc gcgatacggc ttcgggaagc
     7681 tggcaatctg caggttcacg ccgtactcgg tggcgaagaa gggggtcaca tcgtccccgc
     7741 tcagcgcgag ctcgcgcaac gcgcgcacgt ccgcgcccgc acacagccca cgctcgccag
     7801 cgccagtcag ctcaaccgat gaaacctcgg catccgcggc gaacaactcc aacgcgtccc
     7861 cgatcgcgcg catcatcgcg accgtcaacg cgttgatctg ccgaggccgg ttcaacctga
     7921 tccggccgac cccggcccgc acctcgacca gcacctcggc ttcgctcatg cggacgctcc
     7981 ttcggactcg agtcgttcgt tgtgttcctg cacatgccgg tagagcagca ccagcatcaa
     8041 cgccagcgca gcgaaaccga gcgcgaccgc gccattgcgc caggccaggt tgccggcgcc
     8101 gtaggccagc agaatggtgg tcagcgtgac cgcggtgccg atgatccggt tggtcggttt
     8161 ggtcagcggt agcagcaggc caccccaggt caggtaccag ctgttcagcg cgatgccacc
     8221 gaacgcgaaa accaaatacg accaggacag gaacttcacc ggctgccgcc ggcccacgcc
     8281 cagaccaagc cagccgataa ccaccacggt gaggagcagc ccgaggtagc gcagcccgtc
     8341 cttgatccac tgcccggcaa ccggctggcc gaagaactcg aacgccagcc gcaatccctc
     8401 gccgaccaga gtgaacggcg cgagcgtgac gatcatgccg ggaacgtcgg ctgcgtccac
     8461 ccagccgaag ccgagcccgg tcgccagcga gatcgccacg aaagccgcga ccgcggcgcc
     8521 cagtgacccg accagccgga gcatcgcggc ggacgtgtcc ggccagctca aggtcttcca
     8581 cggatgccgg atcagggcca ccggatagaa ggccagcagc gccggctgct tgatacaggc
     8641 cgccaccccg accgtgatcg ccgcccacca gaaccgccgc tcataggcca gccagatcgc
     8701 cagcaccacc aggcccatca tcagcgcgtc gttgtgcgcg ccgccgacga agtcgatgat
     8761 caacagcggg ttgatcgccg agaaccagac tgtcaactgc acgtccgcgc ccatgtgcac
     8821 cgcgatccgg ggcaggaagt agacgatcag cgccaccccg agcagcgcgg gcagccgcat
     8881 ggccatcgcc gagaagtacg ggttgttgcc ggccagcacg accagaccgt ggaacatctc
     8941 caggctcagc ggtggataca tggccgtggt gtagcgccac agccacgcgg tctggtcggc
     9001 gaaagcgccg gggagcacag agatcgggtt cgtgtagggg ttcagcccgt ttcgcagcag
     9061 ccagccttcg gcggcatacg cataagcgtc gtggctgaag atcggcggcg ccagcagcag
     9121 gggcaggctc cacagcaggg tgatcggcca gtgcttgacc tcgtggtgga ggctgggccg
     9181 cagcttgaac caggcttcga cgagcagtgc gaccgcggcc accaccagcg cggtgccgag
     9241 gacgcgggcc ggccagttgt ccaggccgag cgcgcgcatc ggctcccacc aaggactgtt
     9301 ctgcggcagg tatgccggcg tcaacgagcc caggaagagc agcaccgaac ccaggagccc
     9361 acgacgcacc ggacggtggg tccaagcggt cgcgatgtca cctcgcatct ctcggacgcg
     9421 ggcgcgccaa aaaccggacc ggctctgggc cggcctggtc tgcggggtca ctgccactcc
     9481 tccgggctcg ctggcggcat ggacgcaagc ctaccgaggc ccccaccccc cgccagccga
     9541 cggctccggc gcgttcgaac acctggtgaa gaaatcgctc ctggctagcc gcctcgcact
     9601 caccaaccct gcccggaccc ggcgccaaca tctcagattc cggccggcga gtgagcgaat
     9661 gtacgcagtc gtcggaatac ggcgggcatc atcggcgctt gtgatgaagg ctgtcgaagg
     9721 ttgccttgcg ctggaaaggg tagccaacaa ctagacttgc ccgacgcgcc ggcttcccag
     9781 tcggccggcc gcgggaccga gaagagaact catcgcatga ccgaagtgac caccctcgac
     9841 cacgtcgtga tccggttcgc cggcgactct ggcgacggaa tgcagttgac gggtgaccgg
     9901 ttcacccacg acaccgcgaa cgcaggcaac gatttctcga ccctgccgaa cttccccgcc
     9961 gagatccgcg ccccagcagg caccgtggcc ggggtgtcca gcttccagct gcacttcgcc
    10021 gacttcgata tccgcacccc gggcgatcgt cccgacgtgc tggtggcgat gaaccccgcc
    10081 gccctcaaag cgaacctcgg tgacctcgct gccaatggcc tgatcatcgc cgacaccgcc
    10141 gacttcaccg cgcgcaacct cgccaaggtc gggtacggat cagacccgct cacggacggg
    10201 acgctgcacg gctaccaggt ggtggcactc gacctgtccg ggcaggcgtc cgcggcggtc
    10261 gcggagttcg gccttggccg caaggacgcc gtccgcacca agaacatgta cgcccttggc
    10321 cttctggact ggctgtacag ccgtccggtc gagggcacga tcgcattcct cgaacgccgg
    10381 ttcgccacgt ctccgcagat ccgggacgcg aacatcgccg cgctcaaggc cggatacgcc
    10441 ttcggcgaga cgacagaggt gttcaccaac cggttcgcgg tggccccggc acccgcaccg
    10501 ttgggcacgt atcgccagat ctccggcaac cgggcgctcg ccttcggcct gatggccggc
    10561 gcggcgaagg ccgggctgca gctgttcctt ggcgcctacc cgatcacgcc cgcgtccgat
    10621 gtgctgcact acctcgctgc ggccaaggac gccggggtga tgaccttcca ggccgaggac
    10681 gagatcgccg cggtcggggc agcactcggc gcctccttcg ccgggcagct gggcgtgacc
    10741 accacgtccg ggccaggcct tgcgctgaag atggagacca tcggcctcgc ggtgatgctc
    10801 gagctgccgc tggtgatctg tgacgtgcag cgcgccggcc cagccaccgg catgcccacc
    10861 aagaccgaac aggccgatct cacccaggcg ctgttcggac gccacggcga ggccccgctg
    10921 ccggtgatcg cggcccagtc cccgggcgac tgcttcgccg ccgcctacga ggcggtcaaa
    10981 atcgcgatcg actaccggac gccggtgatc ctgctgtcgg acggctacct ggccaacggc
    11041 gccgagccct ggctggtgcc cgatctggcc gacctgccgg agatcgaccc gaagttcgca
    11101 gctgaaccca accgtccgga cggggagttc tggccctacc tgcgcgacga gaagctcgcc
    11161 cgcgcctggg cggtgcccgg caccgagggc ctggagcacc gcatcggcgg catcgagaag
    11221 gcggacggta ccggcgcgat ctcctacgac ccggccaacc acgagttcat ggtgaacctg
    11281 cgccagcgca aggtggatgg aatccaggcc gtggtgccgc cggtcgaagt ggacgatccc
    11341 agcggacgcg cgaaggtgct ggtggtcggc tggggctcga cctacggccc gatctcggcg
    11401 gcagcccgcc gggtgcggcg caccggacgc gaactggcgt gcgtccaact gcgatacctg
    11461 aacccgctgc cggccaatct cggcgaggtg ctcgcagcct acgaccgggt gatcgtgccg
    11521 gagatgaacc ttggacagct ggccatggtg ctgcgggcca aatacctggc gccggtcgtt
    11581 agctacgccc gcgtccgcgg cctgccgatc tcgctgtcgg agctggccaa ggatctgacg
    11641 gagatgatcg acgaggaggc ggcgaagtga ccgcgatccc atccgtaccg actcctgagg
    11701 cgacgtccgg actggccaag gtgccgctgt cgatcagccc gctcacccgc aaggacttca
    11761 ccaccgacca ggaggtgcgc tggtgccccg gttgcggcga ctacgccgtg ctggccacct
    11821 tccagggctt gatgccgacg ctcggcatca agcgcgagaa caccgtgatc gtctcgggca
    11881 tcggctgctc gtcgcggttc ccgtactacg tcgacagcta tggcatgcac tcgatccacg
    11941 gccgggcgcc ggccatcgcg accggcgtgg cgatgagccg tccggatctg gcggtgtggg
    12001 tggttaccgg cgacggggac gcgctcagca tcggcggcaa tcacctgatc catgcgctgc
    12061 gccgcaacgt gaacatcacg atcatgctgt tcaacaaccg catctacggg ctcaccaagg
    12121 ggcaatactc ccccacgtca gagcagggga aggtgaccaa gtcgacgccg gcgggttcgg
    12181 tggaccgtcc gttcaacccg gtctcgcttg ccttgggcgc cgaggccacc ttcgtcgctc
    12241 ggacggtgga ctcgcatcgc gcacacctct ccgaggtgct caccgccgcg atcagccacc
    12301 gcggcgcgtc gttcgtggag atctaccaga actgcccgat cttcaacgac ggcgccttcg
    12361 acgccctgaa gggtgccgac agcgggctga tcccgctcaa gcacggcgag ccgatccggt
    12421 tcggtccgga gaactctagg ggtgtggttc gggacgccgg tgggcacctg cagatcgcca
    12481 acgtcgccga ggtgggtctg gagcgggtgc tggtgcatga cgcccaccac gacgatcccg
    12541 ggcaggcgtt cgcgatttcg cggctgaccg acctggccgg cctggcgtcc tcgccgatcg
    12601 ggatcttccg cgacgtgcag cgtccggcct atgacgatct ggcccgcgat cagctcagcg
    12661 tgctggtcga tccggccgcg cggctcgccc aactgcagga cctgatcacc ggcgccgata
    12721 cctggcaggt ctagcctctc gttcctgccg agggaagaat cggggcatgg acgtcgacga
    12781 gggcgcgctg gccgaagcgg tcgccgaaaa tcagttcagc ggtgtggtct cgctcgccag
    12841 cggcgacgaa ccgccgaggg gaacgggcct acggcttcgc ccaccgggcc ttcgaggtgc
    12901 cgaatcgcgt tgacacccgg ttcgccctgg ccagcgggag caagaccttc accgcgctgg
    12961 cggtattgcg cctcgtggag cagggtctga tcgcactcga cgcaccggtg cggccgattc
    13021 tcagcgacga cctgccgctg gtcgatgcgg gggtgacgat cgagcaactg ctcacctccg
    13081 ccagccccgg caacttcgct cccgattgga accctcgcca ccgataattc gggagcggga
    13141 gtcccattcg ggagcgaagt tggactcgat ctggcgagac ctcagcaggc ctgcggctcg
    13201 gtggcggcca gccggaacca gcggtgggcg gcgaaccgcc gggtgtgcat ggagtactcg
    13261 cgcagcgaga accggctgct cggcaccagc cgggcctccg ggatcgccgg agccggcacg
    13321 cgaaccggca gggtgccgag ttcggccatc tgcgtgaacg tgaccatgtg agtggtgtgc
    13381 attgctatct ccttgaaatg tgcaagtcag ttgagtgggg ccggcccggt gctcgcccgg
    13441 accttgagtt cgaccggcag gaggacctgt cgatcggggt agtcgggatc catcgccagc
    13501 cgtgcggcca gccgtccctt ctcagcgatc ggttggctca cggtggtgag accgaggaag
    13561 ccggcgtccg gtaggtcgtc aaatccggtg atcgacacgt cgcgtcccgg ggtcagaccg
    13621 cgctcgcgga cggcgtccca gacgccgatg gcgagtacgt cgctcaaggc gacgatcgcg
    13681 gtcggccgat gctggtcgtc caggacgtgc gccccggccc tgcgtccaga ctccttgatg
    13741 ttctggccgg cacagaccag tcggatgctg gcgccgggca gcgtctcctg caaaccgcgg
    13801 acgcgctcgg cggcatatcc gacccggacg cccgggaagc cgaccggcca ggctccgcca
    13861 ggagcgagct cgacgtccag tacctcgcgg ccggcgggca gcgtcccggg aacgatcacg
    13921 acgatccggg agtggccaag acgggccagg tgcttgccga ccaggcggcc ggccttggcg
    13981 tcgttgatcg cgacccaatc gccctcgcta ccgcgatggg tgccgacgac gggtacgccg
    14041 cggcggcgaa gcatcccgag gccggggtgc ggctcgaacg gcatcgccgc gaccaccgcg
    14101 tccacggcgg cccgctgcag cacctcactc tcgccgtccg gggaagcgtg cagcagcagg
    14161 accgaggagc cgaactcctc cagcgtctcg ccgattccgg aaagcaggat gacggtgtac
    14221 gggtcggaga acgcgaaggc cagatggctg ccgagataga agccgcagac gtcggtccgg
    14281 cctgatcgca gcgcccgtcc ggcagcgctg gggccgcagt agccgagcgc ctcagccttg
    14341 gcaaagacct gctcgcgcag ttcggccgac agccgatccg gcttgctgta ggcgttggac
    14401 accgtggtga tggatacgcc gagttcggtg gcgatggtct tcatcgtcac tggaatgacc
    14461 gtcctgccca tctctcacca cctgagttga tcgataaagt gaacgttaca ctttatcgat
    14521 caacctggca agccctgaac ggttgctcac cgctgttgtc gatcgctttc accggactgc
    14581 ggatcgcgcg ttgggagggc ggactgttcg tcggctacta cctcgcctac ctggtcttca
    14641 tcgtgctcac cgcagtcgag cacgaccggt tgggttcctt cagccaggac atggctgggt
    14701 tcgtcctgcc gctgaccgtc ctcaccctga tcgtgttgag ccacggggaa tggcggcagc
    14761 ggcgcgcacg gacgagtccg ccccgcccga gcagcccatg agctggctcc gacggctact
    14821 ctgagccgcg tgaccgactt cgatctggac cctgctgtcc gtccgcagga cgacctcttc
    14881 cgccacgtaa acggccgctg gctggccgcc accgagatcc cggccgacaa gcacgccgcc
    14941 ggcgccttca tggaactgcg ggacgcttcg gagcaggcga tcaaggacat cgtcaccggg
    15001 ttgaccggcg ccgagcccgg caccgaggcc cacctggtgg agcacctcta cggcagcttc
    15061 atggacaccg aagaggtgga cgcgcgcggg ctggcgccgc tgactcccct gctggcagag
    15121 atcgacgcga tcgccagtat cgacgacctg ctggactatt tcggccgcag cctgcgtcgc
    15181 ggtctggctg ctccgatcgg agtggaggtg gaggccgacc cgggtgagcc gtcccgctat
    15241 gcgctgttcg tcggacagtc gggcatcagc cttcccgacg aggagtacta ccggctcccc
    15301 gagcacgccg agatcctcag cagctaccgc gaccatgtca accgcatgct catgctcgcc
    15361 ggcatttcgg cgagcgtcgc cggcatcacc gtcgacctcg aaaccgacgt ggccgccaag
    15421 cactgggaca aagtgcgcac ccgcgacatg gtcgccatgt acaacccgac cgactgggcc
    15481 gacctcgatg cagccggggt gccgtggacg cgcgtcctgc tggccgcagg ggtgccgcag
    15541 gtcgagcagg tgatcgtcgc acaaccgtcc ttcctcaccg atctggccgc gctgctcact
    15601 cccggacggc tggacgactg gaagcagtgg tgccgctgga cgctcgtctc cggcttggcg
    15661 ccgttcctgc ccgaagcact ggtacaggag cggttcaact tctacggcac cacgctgcaa
    15721 ggcgtgccgc agctcaagga gcgctggaag cgcggcatcg acctggttga gcgcagcctc
    15781 ggcgaggccg tcggcaagct ctacgtcgag cgccacttcc cgcccgcggc caaggaacgc
    15841 atggacgagc tcgtggcgaa cctgctcgcc gcgtaccgcg agtcgatcac gaagctcgac
    15901 tggatgaccg acgccacccg cgcggaggcg ctgctcaagc tggccaactt cacccccaag
    15961 atcggctacc cgacggtctg gttggactac tcgggcctgg cgatcgaccc acgcgacctg
    16021 gtcgccaacg tgctggcctg cgcggagttc cacaccacgg acgagttgag caagctcggc
    16081 ggcccgatcc gcgaatggga atggctgatg accccgcaga cggtcaacgc ctactaccac
    16141 ccgctgcgca acgagatcgt cttccccgcg gcgatcctgc agccgccgtt cttcgacgcc
    16201 gaggccgacg atgccatcaa ctacggcggc atcggcgcgg tgatcggtca cgagatcggc
    16261 cacggcttcg acgaccaggg ttcgacctgc gacggcgagg gacgcttgcg ggactggtgg
    16321 acgccggccg accgggaggc cttcaccgag cggaccaagg ccctgatcgg ccagtactcg
    16381 gcgctcgccc cggcccagtt gcccgagttg cacgttaacg gcgacctcac catcggtgag
    16441 aacatcggcg acctgggcgg tgcggccatc gcctacgagg cgtggcaact ggctttgggc
    16501 ggtcgggcag ccgagcctgt ggacggcatc ccggccgaac agcggttctt cctctcctgg
    16561 gcgcgggtct ggcagagcaa gcgccgtgac gaagcgctac gccaacagat cgcgaccgac
    16621 ccgcactccc ccgaggagtt ccgctgcaac caggtggtgc gcaatctgcc tgcgttctac
    16681 gcggcattcg acgtgaccga agcggacgcg gcgtggctcg atccgtccga ccgggtcaag
    16741 atctggtgac ccagacgccg gtcacactcg cccggggcgc atacgccgaa ctgcagcgga
    16801 tcaaccggat cctccgccgg gagtcggtgg gcggcatcct gctgctggtg gctgcgctcg
    16861 ccgcgatgct ctgcgccaac ttcgcacccg ggttctactt cggcctgcgc gacctgcacc
    16921 tcggtggcga cctgctcggc ctgcacctcg acctgtcgat cggccactgg gcggcggacg
    16981 ggctgctggc ggtcttcttc ttcctggtgg gcctggaact caaacgcgag ttcgtgaccg
    17041 gcgacctgcg cgatccgaag accgcactgg tgccggtggt ggcggccgtg ggtggcgtgg
    17101 ccgtgccggc gttgatctac tccgcgttca cgttcgccta tccggacatg ctggcgggct
    17161 gggcgatccc cgcagcgacc gatatcgcgt tcgccctggc cgtgctggcc gtgatcggtt
    17221 cgcacctacc ggctgccctg cggacgttcc tgctgaccct cgcggtcgcc gacgacctga
    17281 tcgcgatcac gatcatcgcg ttcttctaca cccgcgatct gaagttggag tacttcgtgg
    17341 ccggactggt gccgatcgtg gcgttcggcc tactggctca cctggcccag cgcttcctgg
    17401 tgcggcacgg ctgggcggcg ttcctgctgc tactgccgat cgggctggtg gcgtgggcgc
    17461 tggcctacaa ctcaggggtg cacgcgactg tcgccggcgt gctgctggcg ctcggcgtgc
    17521 cggtgcgtcc ggccaagggc gataccgccg agcatggcct ggccgaggtg ctggagcaca
    17581 aggtgcggcc gttctcagcc ggagtcgcgg tgccggtgtt cgcgttcttc tcggccgggg
    17641 tgctggtggg gggcttggac gggttcgtgg cggccgtgac cagcccgatc ggattgggcg
    17701 tgatcgtggg cctcgtcgtg ggcaaggcga tcggcatctc gggcgccacc tggttggtga
    17761 cgcggttgcg gcacgcccag ctggacccgg atctggcctg gatcgacgtg ttcggcctag
    17821 ccgtgctcgg cggtatcggc ttcacggttt cgctgctggt gagcgagctc agcttcggcc
    17881 agggcacacc gcacgacgac atcggcaagg tggccatcct gaccgcgtcc gtgctcgcgg
    17941 ctaccctggc cgcagtgctg ctcggttccc gcaaccgcca ctaccgcgcc gtcgaagtcg
    18001 ccgaacaggt ggacgccgac gctgacggtg tacccgaccg ctttcagcac tgacgaccat
    18061 tggacctgac gacgggccgc cgccactatc caatcccgac ggccacctca gcgctgtata
    18121 cgtcccgtcg atgaccagag ggttacgacg acgatcagca acacttcgtc gtcgattgtg
    18181 tagaggatgc gatagtcgcc cacgcgcacg cgaaggccat cccggccacg gagtgcgcgc
    18241 gccgggcggg cgggggttct cggccagcag tgcgatctct ccgtcgatcc gtccgcgatc
    18301 tctcgccgct atgtgacgta gcgcattgag cgccgcgagc cgcacttcga cccggtaggt
    18361 cacacccagc ccagatcggc cttgacctgt gcccagggga tgttcggacc actctccgcc
    18421 ggcgccgcgg cgagttggtc tcgaccggct cgaccagccg gatcgatgga tgtgctgcgc
    18481 ttagcccttg gggtcggaaa ccaggaacgg atgctcccag ccgactgcgg cctccagctg
    18541 gccggcgagg ctcagcagca gctcctcggc accgtgggcg gcgccgaaca tcactccgat
    18601 cggcactacc gctccgtcca cctcggcagt gtgcagcggc agggagatcg ccgggcctcc
    18661 ggacaggttg tagacgctcg tccacggggt gaagcgggtc tgggcggcga agtcctcggc
    18721 ggggtcggcg tcattgcgca gcgagccgac cagcgccggg ggttgggcca gcgtcggcgt
    18781 gaggatcaca tcgaaccctg cccaggcccg ggcgatgtcc accccgatcc gctggacggc
    18841 ggccgcagcc cgcgcgtact ccaagccagt tgcctgccgg ccgcgctccc gcagccatcg
    18901 agtgagcgga cgcagctccg cctcggcctc tgccggcagt ggcaccatcg ccgcgcccac
    18961 cgcccacagc tcggagaacg cctcccagcg gtccacagtg aacggctgcg gcgcggccac
    19021 cacctcgtgg ccaagctcaa gcatcgtggc cgccgtccgg tcgaccgcgt ccagacaagc
    19081 cggatgcacc tcggccacag cgatcgcggg ctcgaccagc acaccgatcc ggaggctccc
    19141 cggcgatcgg gtgaccgcgt cgaggaaccg gcccgagggt gccgccgcca catacggctc
    19201 acctatccca tggccggcca gcacatccag ggcagccgcg gtgtcagcca ccgtccgact
    19261 cagcaccccg tcggtcccca accccgggcc gggctcccgg aatcggccgg tgctcacccg
    19321 gccacgactg gccttcaagc ccaccagccc gcacgccgaa gccgggatcc gaatcgagcc
    19381 gccggcgtcc gaggcgtgcg cgatcggcgc cagccccgtc gccaccgcgg cagctgcacc
    19441 accgctcgag ccgccggcag agcggcgcac gtcccacggc gtgcgcgcgg ccgggccggt
    19501 ctccggctcg gtgtagcacg gcagcccgaa ctccggggtt gcggtcttgc ccagcagcac
    19561 cgtgcccgcc tcgcggaacc agctcacgat gtcgtcatcc acctcggcca cgtagccggc
    19621 catcgcggcg ctgccggctt cgaagccgac gcccgcgacc ggactgaggt ctttgatcgg
    19681 gcagggcacg cccaacagcg caccgtccgc ctcaccggaa cgaacgcgag cgtccactgc
    19741 tgcggcctga gcgagggcca actcgggcgt cagtcgggcg aaggcgccga ccgtgtcgct
    19801 caagcgatcc gcgcgggcca ggtgtgcctg cgtcacctcg accgcagcga aatcaccccg
    19861 gcggacgccg cgggacaccg ccaacgcgtc cagttcagtc agttccgtca tggcttgatc
    19921 ttgtcaggtg ccagcgtccc ggtgatcgtc ggctggaagt cgtcaccgcg ttgcagcacc
    19981 gcgaggcgtc cgaccatctg ctgctcggtg atcacccgct cgcagcggcc gcggccgatg
    20041 aacgtgatcg cccagtgcag cagcgtcgtc agccggttct tgaagccgac cacgtagatc
    20101 aggtgcacga acagccaggc gaaccccgcc gggaagccgg tgagttccag ccggccgacc
    20161 ttggcaaccg cgccgaagcg gctgatcacc gccatcgacc ccttatcgtg gtacttgaag
    20221 tccgcggtca ccgggtggcc ggtttgctcg ccgatgatct tgctggccgc gtaccgcgcg
    20281 ccctggatcg ccacttgggc aacgccggga aggttgtcca gcgcgatcat gtcgcccagc
    20341 acgaacacct cggggtgccc gggcagggtc agtccggatt cacctgcacg cgtcccgcgc
    20401 gatcgacacc ggcgccggac tgctcggcga gcgtccggcc aagcgggctg gcctgcaccc
    20461 cggcggccca caccttgcag ctggcctcga tccgctcggt ccggccgtcc tggtagcgca
    20521 cctccagtcc gttggcgtcc acgtcggcga ccagggcgcc cagccgcacc tccaccccgt
    20581 tgcgctccaa ctcgcgccga gccgcgaccg agagcttcga gccgaatggg gccagcaccg
    20641 cgctggcgcc gtccagcacg agaatccggc tcttcgtggt gtcgacccgc cggaagctgc
    20701 gccgcagcgt ctgccgggtc agttcagcga tctggccggc catctccacg cccgtcggtc
    20761 cggcaccgac tatcacgaag gtggtcagtg agcgccgctc ggcttcgctc tcggtgagct
    20821 cggccatctc cagcgcccgg acgccccgga ccaatcgact ggctgaccag caatcaggcc
    20881 gtctgcaggc tcgccgccgg ctcgaagtgc accacctcac gcagttcgtg ggactgcacg
    20941 caggcttcga gcaggcgcat cgtccgcagc acctgggcgt tggtgatcac cggctcggca
    21001 ccagcgtcca ccaccgcgag tgcgttgcgg tagaagtcgc ggacgtccgc ctcgaccacc
    21061 ggcaccggca ggtcctccag cgactcgtcc agcttgtagc tgttggagta gtccttgacc
    21121 cgcggcgcca tggtcttggt catgccggcg ccggcctgga tcggcgcggc atcatcctcc
    21181 tcgaccacgc cgagccgcag gatccgtccg ggcgtcgccc agttctcgat gcgagccgag
    21241 ccgcgattgg cctgcaatga ccacaggggc atcggctgga agctggtcgt gctcacatcc
    21301 accagggcgg tcacgccgtt gccgaaagtc agataggcct tgaagccgtc gtcgacttcc
    21361 tggcccaggg catagctgag gttcgcgtag acgtcggtca cgggcgcgtc caccatcagc
    21421 aacagccggt cgaccaggtg aacgccccag tccagcagca tgccgccgcc gttctctttc
    21481 ttcgtgcgcc agtcggacgg gatgccgcgc gacccgtgca ccctggtctc cacataggtg
    21541 accggaccga tcgtcgcctc gtcgtagacc gccttcatgc tcaggtagtc cgggtcccaa
    21601 cgccggttct ggtgcaccat gaacagtttg cccgcctgct cggcagcggc gatgacctcc
    21661 accaactcga ggctcgtgac catcgccggc ttctcgcaga gcacgttctt gccggcggca
    21721 agcgcggtca tggccaggtc gtggtggctg tcgttcggcg tggcgatcat gacgaagtcg
    21781 aactcgagtt cggcgatcgc ctccggcagg gacgcgtacg cgtgcacgcc ctcatcctgc
    21841 gcggcagcgc gggcctgctc gtcgatgtcg accacgccga cgacctcgaa gccgtcgatc
    21901 gtcgccaggt tgcgccggtg gtagtcgccc atcgtgccgt agccgacgat caccagtggg
    21961 tggctcatgc tggttccttc gctgttggtt cttgccgccg ggcgatcacc gcccggcctt
    22021 gccgacggcc cgcgtccact ccgaacccgg gccgccgacc ttgctgctac tactcgccgg
    22081 gctgcacctg ggtgctgaac gcggcgtcga aggatgcggt gggtgcgtcg aacaggttgg
    22141 cccgaacgaa gtcgatggcc tccttgccgc cacgcagccg gtccatcccg gcgtcctccc
    22201 actcgatgct gatcgggccg gtgtagccga tcgcgttcaa cgtccggaaa caccgctgcc
    22261 acggcacatc accattacca gtcgacacga acgtccaacc ccgccgcgga tcaccccacg
    22321 gcaaatgcga acccatccga ccattccgac cattaccgca agccatcttc gtgtccttgc
    22381 agtccacgtg ataaacccga tccgcaaaat ccaccaagaa ccccaccgga tcaatctcct
    22441 gccacatcat gtgcgaggga tcccagttca acccgaacgc ctcccgatgc ccaatcgcct
    22501 ccaacgtcag ctttgtcgac cagtagtcgt aggcaatctc ggagggatgc acctcgtgcg
    22561 cgaaccgcac cccctcggaa tcgaacacat ccagaatcgg attccaacgc cgggcgaaat
    22621 cggcgtaacc atcctcgatc acgctgtcgg gcaccggcgg gaacatcgcc acgtactgcc
    22681 aaatcttgct gccggtgaac cccaccaccg tgtccacgcc aagcttccgg gcagcgatcg
    22741 cggtcagctt catctcctca gccgcacgct gccgcacccc ctcggggtcc ccgtcgcccc
    22801 aaacggcgtc ggacaccatg tcgcggtgcc ggaaatcgat cggatcatca caaaccgcct
    22861 gacccttcaa atgattgctg atcgcccaca ccccaaggcc gtacttcgcc aacgtggcct
    22921 tcttctccgc cacataagcg tcatcggaag cagcacggta gacatccaga tgatcacccc
    22981 aacaagcaat ctccaaaccg tcataacccc aacccgaagc cagccgacaa acctcctcaa
    23041 acggcaaatc agcccactga ccagtaaaca aagtgaccgg acgcgacatc ttcgctactc
    23101 cttgttcaga acttgaccgg ctcgccggag gccgacgagg catagatggc ttcgaggatc
    23161 tcggtcaccc ggcaggcctc gtcgggcagc acgaccggat cggtgccgtt gcgcagggct
    23221 tccagccact gcctggtctc agccgcgccc agccgggcga agtcctccga gccggggctg
    23281 ctgggggtcg gttcggaaac gatcatgcgg ttggcgagca ccttgttcac cgtcgcctca
    23341 tagcccttgc cgtggtgctc gatctcggcg cccccgtcgg tgccgatcag ggtgaccgcg
    23401 gcctctcgcg gatccttgac gttcagcgcc caggccgcct caaggaagat ggtggcgccg
    23461 ttcttcatcc ggatgtagcc gaaggccgaa tcctcaacgc tgaaggtttc cggatcccag
    23521 ccgccgccgg ggttgccctc cggcttgtca cgcagcttct cgaagaccga accagtcacc
    23581 gagtcgacct cgtagttgcc catgtaccag agcgccaggt cgagggcgtg ggtgccgatg
    23641 tcgatcagcg gaccgccgcc ctgcttctcc ttgtcggtga acacgcccca ggtcggcacg
    23701 ccgcgacggc ggacggcgtg ggccttggcc acgtaaatct cgccgagctc gccctcgtcc
    23761 acgacatcgc ggaggaactg cgcgtcctca cgcagccggt tctggtaccc gatggttagc
    23821 ttgcggccgg tgcgacgggc ggtctcgacc atgcgccggg cgtcctcgcc ggtcacggcc
    23881 atcggcttct cgcacatcac gtccttgccc gcttccaggg ccgcgcaggt gatctcggcg
    23941 tggctgatgt tccaggtgca gacgtagatc acgtcgatgc tctcgtcggc gacgacttcg
    24001 cgccagtcgg tggtcacgta ggcgtccggg gtgccacagc gcgccttgcc cttctcggcc
    24061 cggtcgagct gcaggtcaca gaacgcgacc acgtcgcaga ggtcggcgtt ggcggccagg
    24121 ccctccaggt gcttgtaatc ggcgatgaag ccgacgccaa tgatgcccac tcggtactta
    24181 ctcactgatc tagctccgtt cggatcgcgg ccggtactgc actgtaacgg cccttgctgg
    24241 aagttatgag agccctctca tcctcaggct cgattctgcc ctaccgggca ccctcatgga
    24301 cactcgtggg acggatcggc gccaatttcg cgctgatctg ccgagtagca accggcagcc
    24361 gtccgacttc gctccccgag cgggcccgtt cgcgccagcg attcggacgt tcgccaggga
    24421 accttccccg gcgaacgtcc gaatcgctgg cgtaaatgag tgcccttcgg gagcgcagca
    24481 gcgggaggaa atgcggacgc ggaacttcgg gccggcccgg tgcgcggacg gacctcagga
    24541 cgagcgcgag atgacgccgt cgcacagcga agacagcgcc tgcttgaccg gcccctcggg
    24601 cagcggcgcc aggtggcccc gggcgatctc ggcgcgggtg tggatcactt ctcgggcttc
    24661 ggccatcgcg gggtgcgcgc gcatcagacc gagggcttcg gcgagatcgg cgtccgcgct
    24721 caggtcggcg tccaacagag ccaacagccg ggcgtccgac ggctcggccg agcggcgggc
    24781 cagcagcgtg ggcagggtgg ggacacccgc gcgcaggtca gtgcccgggg tcttgcccga
    24841 ggtatcggat gcgatgtcga tgatgtcgtc gctcagttgg aagaccacgc cgatctcctc
    24901 gccgtaggcg gccagagctg ccagctcgtc cggcgacacc gcggccacca tgcccccgaa
    24961 ggtcgtggac gcggcgatca gcgagccggt cttgtccgag accacgtcga ggtagtgctc
    25021 caaccggtcg tcgccgtcca gtggtcccct ggtttcggcg atctggccct gcaccagccg
    25081 ggcgaaggtg tcggcctgca acttcacgta ctcggtgccc agtgtcgcca ccagtgcgga
    25141 cgcccgggcg aacaggaagt cgcccaccag gatggccacc gtgttgtccc agcgctggtt
    25201 ggcgctcggc gcaccgcggc gcaactccgc ctcgtccatg acgtcgtcgt ggtagaggct
    25261 ggccacgtgg gtgagctcca tcaccagcgc agcgcggatg atcaactcgt cgtccgcgtc
    25321 tgcacgcagg agcgcggcga gcaccaccag cagcggacgg aaacgcttgc cgccggcgct
    25381 gatgatgtgc tgggccgcct cggtgacgaa atcggtctgc gcgatcgccg cgtccgcaag
    25441 gcgctgctcg atgcgttcga gcagttcggt gacacgctgg gtgaattgcg gatccaactc
    25501 acccacgaga tcggcgggct gggtggctac tggcacgccc gacagcatag cctcaggctc
    25561 agcgcaggaa cgtgctggcc gacgtcacca ggtcgagcag cggcccgggg aacagcccga
    25621 ggtacgcgga cgcgaccgcg cccagcacca ccggcaccca ggtcattccg ctcgccttgc
    25681 ccacgccgac gccgagagcc ggctcggcga agaacatggt gacgatcacc cgcaggtaga
    25741 cgtaggcggc caccaggctg aacgcgaccg cggccacgac cagccaggag tacccgccgc
    25801 gccaggccgc cgagaacacc gcccacttgc cgatgaagcc accggtcagc gggatgccgg
    25861 cgaagctcag catcagcagc gcgaagatgc cggccagcca ggggtgctta cggccgagcc
    25921 cggcccagcc cgagatggcg tgcacctcgc cggccgaatc gcgcaccatg gtcacgatcg
    25981 cgaaggccgc gattgtcgcg aagccgtagg ccaccaggta gaacaggatc gagccgaagc
    26041 tgttcacctg gccggtcaca ccggcggtgg cgccgaccac ggccaccagg atgaaaccgg
    26101 cgtgcgagat ggagctgtag gccaacagcc gcttcatgtc ggtctgggtc agcgcaacca
    26161 ccacgccgat gcccatggtg agcaccgcga ccgtggccag cagcggctgc cacgtccagc
    26221 gctccgcgcc gaaggccacg aagaacaccc gcagcgtggc accgatcgcg gccagcttgg
    26281 tgcagatcgc catgaagctg gtcaccgggg tgggcgcgcc aacgtagacg tccggaaccc
    26341 agttgtggaa cggcaccgcg ccgatcttga acagcaggcc gatggccagc agcgcgatac
    26401 cggtgaagag cagtccacgg ccgtagaccg ggttggcgat ggcgtcattg atcgcgctca
    26461 gttggaacga cccggagaag ccatagacca gcgccatccc gaacaggaag aacgcggacg
    26521 acaacgcgcc cagcaggaag tacttgagcg cggcctcctg gctcagcagg cgacgcttgc
    26581 gcgccatccc cgacaggagg tacagcggca gcgacatgac ctcaagggcg acgaaggccg
    26641 tgatcaggtc gttggccgcg acgaagacca gcatgccggc cagcgagaac atcgccagcg
    26701 ggaagacctc ggagtgctca tgccgggccg aattggcctc ggcctcggct gggctgcccg
    26761 gcaccgaagc agcggacgcg gcgaaggcgg tggccccgtt gtcgaggtca cgctcggaaa
    26821 agaccagcag ggcgagcaga ccgaagacca gcagggcgcc ccacatcagg taggtcgggc
    26881 cgtccagcga gatcgagccc atggccagta ggccctggct gtgctcgggc tgccgccagc
    26941 tgatcaccag gaacgcgaag gcgacaccga tcgccacctc gaaaagaccg atctgggcga
    27001 ggaagcggag gcgccgcggc agcacggctt caatcacgat gccaaggcag gccgccagca
    27061 ggatggtcag gacgggcgct agctgcagcc actcgaacgt tggcgcggca atgcttttca
    27121 cttggcttcc cctcccagcg gagccaccgg gtcagcgatc ccgacgatgc tcatcgtctg
    27181 gtgcgcggtg ggttcgatga cgttcaaagc gatgttcggc aggaaaccga gcagcaggat
    27241 caccgccagt agcgggccga tcacccactt ctcggacgcg gtcgcgtccc gggtgaagtg
    27301 ctcagccacc gcaggggtga ccgggccggt catggtgcgc tggtacatga gcaggatgta
    27361 gaccgccgcc agcaccgtgc cgagcgaagc gaccgccccc acccacggaa tcgccgacca
    27421 ggcgccggcg agcaccatga actcggagac gaagctcgac atgcccggca gtgccaggcc
    27481 ggacagaccg gccatcagga agacaccggc gagcaccgga gcgagtttct gcacgccgcc
    27541 gaaatcggcc accgcagccg agccgcggcg cgagatcagg aagccgatca ccaggaacag
    27601 cgcggcggtg gagaagccgt ggttgaccat gtagaagatc gagcccgaga tgcccacact
    27661 ggtgaaggtg tagatgccca gcaccatgaa accgaagtgg ctgatcgagg tgtaggccac
    27721 caggcgcagc aggtcgctgc tgccgatcgc ggcgatcgcg ccgtagatga tgctgaccag
    27781 ggccaggccg atcatcaccg gcgccaccca ggtgctggct tcggggaaga gcccgaggca
    27841 gaaccggatc atgccgaacg tgccgatctt gtccagaatg cccaccaaca gcaccgacga
    27901 cccaggcgtc gcctgctcgg tcgcggtcgg cagccaggtg tggacgggca ccatcggcgc
    27961 cttgacgatg aacgcgacca ggaagcccag catcagccag cgtcccagca tcgggtcgat
    28021 ctgcagctgc tggagctcgc tgagcaggta ggtcggcgct ccagtggcct gcaccgacca
    28081 ggcgtagatg ccgatcaccg aagccagcat gatcaggcct ccggcaaggc tgaacagcag
    28141 gaacttcacc gccgcatagg cccgcttggg accgccgaag ccgccgatca ggaagtacat
    28201 cgggatcagg gtggcctcga agaacaggta gaagagcagc acgtcctcgg ccatgaaggt
    28261 gaagatcgag aggctctcca gcgcgagcac gagggcgaaa aagacctggg gcgaccagcg
    28321 tccgccgccc ccgacccgcc attcggccag cagcaccacc ggcaccagca ggacggtgag
    28381 tagcaccatc accagcgcca tgccgtccag accgacggcc cagccagccc cgaaggccgg
    28441 aatccaggga gcgcgttcga cgaaggtctg gccggccagg tagaggccgc cgataacaag
    28501 gccgatcacc gcggtgatca gggcgaacgc catgcccacc cagcgggcgg cgtgcttgag
    28561 cggcaggcag agcacggctg caccgagcag cggtgccaga cccaggaacg tgagccaggg
    28621 gaatgtcatt gtcggctacc tccctcagcc cagctgactg agaatcatca cgacgccgac
    28681 cagcacagcg ccgatcgtca tggtgagtgc gtacgttcgg acctgcccgg tctgcagctt
    28741 gcgggtctgc gtggacaacc cgttgaacat gtcggccacc ttgtcggcgc cgacatcgat
    28801 gatccggtcg tcgttggcga ccaggccgtg agcggccacc atcgtcggct cgaccacgag
    28861 aacgttgttc accgcgtccc cgaagaggtc gttgcgtccc atcaccgtga acgggttggt
    28921 ggacgcgggc gcgaccgccg gtatcggctt ggtaccgaag agccaccagg ccagcgccac
    28981 gccagcggcg acgagaagca gggtgagcca gccgatcggg gtcggaatca gcgacacctc
    29041 gtgggcgtgg gcaccggtgg ccggctccag ccagtgctgg atccagccgt tcatcaacgc
    29101 gccggcgacc accgacagcg ttgccagcac gatcagcggg acggtcatcg tccgcggcga
    29161 ctcgtgcggg tggacgcccg gctgccagcg cttctcgccg aagaaggtga acagcatcag
    29221 ccgggtcatg tagaaggccg tgatccccgc gccgatcagg gccgcggtgc cggcgatcgg
    29281 gctgtgctcg aaggccgcct cgatgatgtg gtccttggag aagtagcccg cccagcccgg
    29341 gaagccgatg atggccagat agcccatcgc gaacgtccag aaggtgacct tcaccacctt
    29401 cgccagcgcg ccgtagtggc gcatgtccac gtcgtcgttc atcgcgtgca tcaccgaacc
    29461 ggcaccgagg aacatgttcg ccttgaagaa accgtgggtg agcaggtgga agatggcgaa
    29521 cgcgtagccg gccgggccga tgcccgcggc cagcatcatg tagccgatct ggctcatcgt
    29581 cgaaccggcg agcaccttct tgatgtcgtc cttgctggtg ccgatccagg caccggccag
    29641 cagggtcacc gtgccgacca cgaccacggc cgtgctggcc gccgacgaca tctcgaagat
    29701 cgcgtggctg cgcaccacca ggtagacgcc ggcggtgacc atcgtggccg cgtggatcag
    29761 ggccgaaacc ggggtcgggc cctccatcgc gtccagcaac caggcctgta gtggtacctg
    29821 ggcggacttg ccacaggcgc ctagcagaag cagcaggccg atcgtggtgg cccagaacgg
    29881 atccaggtgc tcggcgttgg cgttcaccgc cacgaaggcc gaactgccga acagggccag
    29941 catgctcatc acggccatcg tcatgcccag gtcgccgacc cggttcatca cgaaggcctt
    30001 cttggccgca gcggcggcgg acggcttgtg ctgccagaag ccgatcagca ggtaggacgc
    30061 cagaccgacg ccctcccagc cgacgaacag caccagatag ttgtcggcca gcaccagcac
    30121 cagcatcgcc gcgatgaaca ggttcaggta ggcgaagaag cggcgccggc gctcgtcgtg
    30181 ggccatgtag ccaaccgagt acaggtggat cagcgcgccc actccggtga tcagcagggc
    30241 gaacaggatc gagagctggt cgatcaacag accggcgctc acctgccagc tgccgagcgt
    30301 gatccactcg aacatgggca ccgcaaccgc gcgggcgtcc tcgggtgaga gcagctgctg
    30361 gctgaacagg cagaccgcga tgctgaacga gaaggtcacg gccgcgaccg cgatccagtg
    30421 accgatccgg tcgagaatgc tgcccgccag cagcagcagc gccgccgaga ccgcgggaac
    30481 ggcgatcatc agccaggcgt aggcgaacat gccggtggca gccaccggag ccagggtttc
    30541 cagagtgatc attcggccct cacagcttca gcaggcttgc cgcgtccact gaggtggatc
    30601 gccgggtgcg gtagatggtg atgatgatgc tcaggccgac cacgacctcg gcagcggcca
    30661 ccaccatcac gaagaaggtg gccacctggc cgtccagacc gccgcgggcc atggcgaaag
    30721 cgaccatcgc caggttggcc gcgttcagca tcagctcgac cgacatgaac gccaccaggg
    30781 cgttgcgccg gaccatgacc ccgaccgcac cgatcgtgaa caggatcgct gccaggtaga
    30841 ggtagttgtc ggggctcatc actcgtcctc ctcgccctgg tgggactcga tcgactcgaa
    30901 ggcccgggcc gtggccaggc tggccgcggg cgcgtccacg atcacgccgc gtccggccag
    30961 ggtcggcgag attgacgcct cgctgaccgt gccgtccggc aacagggccg gtgtggcgat
    31021 cgagttgtgg ttggccagaa caccggagtt cggcagcggg ccggggtgcg tgcccaaggc
    31081 cctgtagtcc cgcatgcgct tctcggcgag ctccggctgc gtcgctcgcg gccggacgcg
    31141 gaagtgctgg cccagcacca tcgcaccgac ggccgcggtg atcagcagcg cagaggtgag
    31201 ctcgaacacg aacacgtagc ggccgaagat cagtgcggcc agcgactcga cgttgccgcc
    31261 gtgggccgcg ttggccgcat ccagcccgat ccggggaccc ccgacgagcg agccgccgat
    31321 gccgaacaga atcaggccga gcaggcccag gccggccagc ccggcgagcc aacgctgccc
    31381 gggaatggtc tcgacgagcg aatcggcctg gtgtccgatg ccgaccagca tcagcacgaa
    31441 caggaacagc atcatgatcg cgccggtgta gacgatgatc tgcacgaagg tgaggaacat
    31501 cgcgtccatc gaggcgtaga ggacggccag gttcaccatc acgaaagcca tgctcagcgc
    31561 ggcgtacacg gccttacgga agaccagcag tccgagcgac cccagcacca tcagcggcgc
    31621 cacgatccag aaggtgacct ctgctccggt cacgagtgga atcatcgagt ccctccaggg
    31681 gtgtccgtga ccaccggcgt ccgattgtcc ggcttgccgg tagcaggcag gccgaggaag
    31741 tagtccttct cgtcggcgcc cagccggcgc gggtgcggcg gctcctccat gcccggcagc
    31801 agcggtgcca gcaaccggtc cttggtgtag atcatcttct ccctggtggt gtccgccagc
    31861 ttgaagtcgt tggtcatcgt gagcgcccgg gtcgggcagg cctcgatgca caacccacac
    31921 aggatgcagc gcaggtagtt gatctggtac acgcggccgt agcgctcacc gggtgagtag
    31981 cgctcctcct cggtgttgtc cgcgccctcg acgtagatcg cgtccgcggg gcacgcccag
    32041 gcacacagct cacagccgac gcacttctcc agcccgtccg gccagcggtt cagctggtgt
    32101 cggccgtgga agcggggcgc ggtgatcttc tccttgccct tctccgggta gccctgggtg
    32161 aaggtcttgc ggaagatggt ccggaaggtg actccgaacc cggcccacgg gtcgaagatg
    32221 cccattactg ctcctctcct gccggctgtg gcgccgcctc tgcggtggac gcgaccacgt
    32281 cctgcagttc cggcagtacc tgccctggca tcggtgggac gggatagccg cccgcgaacg
    32341 cgtcgaactc cccctggctg gcaggctcgg gctcgggctc ggcactcggg ctccagagca
    32401 tgaacgcgac cagcagggcg aggatgatcc cgatcgccaa ccacagcacc ggcccctgca
    32461 gcaggttctc ctgccgcagc gactggaaag tggcgatgat gatgatccac accagcgaga
    32521 tcgggatcag cgccttccag cccagcttca tgaactggtc gtagcggaag cggggaaggg
    32581 tgccgcgcag ccagacgaac agcgagatga acagttgcac cttgatcgcg aaccagatcg
    32641 ggccgagcca cggattgttc agtaccggga tcaggttcag cggccagaac ggcaggtagc
    32701 cgcccacgaa gagcgtggtc gcgatcgccg agaccgtcgc catgttgatg tactcggcga
    32761 ggaagtagac cgcgtacttg aacccggagt actcggtgat gtagcccgaa accagttcgg
    32821 actcggcctc aggcaggtcg aagggtgccc ggttggtctc gcccaccatc gagatcaagt
    32881 agatcacgaa gctcggcagc agcaacagcc agtaccagga ctgcagcccc acccattccg
    32941 ggccgagcgg cgcacgctgt gcctcgacga tctggtgcgt cgacatcgag ccggcgtaga
    33001 ggaagaccga caccagcgag aggcccatcg cgatctcgta ggagatcatc tgcgcggacg
    33061 atcgcaggcc accgagtagc gcgtacggcg agttggacga ccagccggcc agcacgaagc
    33121 cgtagatgcc gaccgaggtg accgccagta gcaccagaac gccgaccggc aggtcggtga
    33181 gctgcagcag cgtcttcacc ccgaagatct cgacctcacc gccgaacggg atcaccgccc
    33241 aggctgtgaa ggccgcggtg ccggcgatca gcggtgcgat cacgaacagt gcgcgatcga
    33301 ccatcgaggg catgaagtcc tctttgatca ggagtttcat gccgtcggcg agcgcctgtc
    33361 ccaagccaaa cgggccgttc atgatcgggc cgaggcggtg ctgcatcttg ccgaccagtc
    33421 ggcgctcgta ccagacgttg aagatcgtcc agagcaatag gacgacgaac agtacgacga
    33481 ccttgatcag taccagccac cacggatcgt tcatgaccag cctcccgcgc tgatggcgac
    33541 gacatcgccg tggacgagtc cgagttcgcc aagtggcgtc ccgggcgagt tcgtgggcac
    33601 ccagaccgtt ccgggggcga gatcgggggt gatctgcacg ggtagggagt accagccagt
    33661 cgcaccaccg acggtgacct cgtcaccgtc ggctacgccg agtcgtgccg ccagttcact
    33721 gcccacccgg gccacggtcg gacgcgcggt ggccgcgagg gccgcttcgc cgtcctggcc
    33781 gcggccaccg tcgatcagct gccgccaagt ggctagcttg gctgccaccg accctgcgtc
    33841 gagttcgggg agcgtgtccg cggtcggact ggtcgggacc ttcttggtct tcgacttggt
    33901 cctggccggc ttggcgtcga ccatcggcgt ccaggcgccc aactcagtga gctcggccag
    33961 cgcagcgctc ggcgtccgga tgcccatggg cctgcccagc gcgtcggcca gtgcggccag
    34021 cacccgcagg tcggtcatcg gcggggtctg gcccttgatc acggtgttca cgcgtccggg
    34081 tcggtgctcc cagttgagga aggtgcccgg gcgctcctcg atcagcgcga ccgggaagac
    34141 gacgtctgcc cgttcggtga cctcggagag ccggttctcc aacgcaacca caaacccggc
    34201 ggcttccagt ccggcccggg ccgccgcggc gtccgcgaag tcgaccggct ccaggccgga
    34261 gaccagcagc gccttcagct ctccggacgc ggctgcggcc agcatcgccg gcgcgtccag
    34321 ccccgtctcg gtcggcaggc tcgcgctgcc ccaggcagcg gccacgtcga cgcgcgcgct
    34381 ggcgtcgtcc accggacggc ctccgggcag caggcccggc aggcagccgg cctcgaccgc
    34441 gccacgatca ccggcccggc gcggcaccca ggccagccgg gcaccagagg ttgccgcgac
    34501 ctctgccgca gcggccaggg cgtcgggagc gagcgctgcc cgctccccca ccaggatcac
    34561 gttgcccgca tcgagggtga ggccacgcag ggcggccgcc tcctcgccgg gagcggtgac
    34621 gaccagttcg gcgttcagct tggcgctacc agtgctcgca tacggcgcga ccgcggtcac
    34681 cttcagcccg gacttgcgga cgcccttgcg caggcgaagg aagatcagcg gcgactcgtc
    34741 ctcgggctcg aagccaacca gcaccacgcg tccggcggcc tcgagttcgg agtaggtgac
    34801 gccatcaccg agcgcgcgtc cggctacctg tgcggccagg aactcggcct cgcccgcacc
    34861 gatcggacgc gagcggaagt cgatgctgtt ggtgccgagc accgtccggg cgaacttgct
    34921 gtagccgtag gcatcctcaa gggtgagtcg tccgccggtg agcaccccaa cgctgctgcc
    34981 ggccgcagtc agtccggcga cggcggcatc aatggcctcc ggccaggagg cgacccgcag
    35041 ttcgtcgccc tcacggatca gcggactcct cagccggtcg tccccacgcg cggagacgaa
    35101 ggcgaaccga cccttgtcgc agttccattc ctcgttgatc tcgggagcgt ccccagcgag
    35161 gcgtcggcgc acctggaagt gccggtggtc gacccgcagc tggcagccac cagcgcagtg
    35221 ctcacaggtc gatggcgtgc tgaccaggtc gaacggacgc gcctggaagc ggtaggcggc
    35281 cgaggtcagc gcacccaccg ggcagatctg cacgacgttg ccggagtagt aggagtcgta
    35341 cggttccttg gcgtacttgc ccacctgctg cagcgccccg cgctcgacca gcgcgatgaa
    35401 cgggtcgcca gagatctgct cggagaagcg ggtacagcgc gcacacagga cgcagcgctc
    35461 gcggtcgagc aggatctgcg ccgaaatcgg caccggcttg gggaaggtgc gcttcacgcc
    35521 ctcgtagcgg gactcgccgc ggccgttgga cagcgcctgg ttctgcagcg ggcactcgcc
    35581 gcccttgtcg cagatcgggc agtccagcgg gtggttgatc agcaggaact caagcatgcc
    35641 cttctgtgcc ttctcggcca ccggcgagga cagctgggtc ttgatctgca taccgggcgc
    35701 gcactccagg gtgcaggacg cctgcggctt gggcatgccg cggccgttgc ccatgtcggg
    35761 cacctcgacc aggcactgcc ggcaagcgcc gaccgggtcg agcagcgggt gatcgcagaa
    35821 ccgcgggatc gcgatgccga tcatctcggc cgcgcggatg gccagcgtgc ccttgggtac
    35881 ctgcacctcg gtgccgtcga tggtgaccgt gatggtctcc accggcgcca cctggtcggg
    35941 cttggtgacc acgctcatgc gtgcgctccc ttcgtgctcg tcgccagttc gcgatcgggc
    36001 acctcgaaca aggcgctgcg gccgtacggg aacagctccc aggccggcgt gctgtagccg
    36061 gcctcgaact cgccccggaa gtgccggacg gcggacgtga cgcaggccac ggcaccgtcc
    36121 gctagcgcgc agaacgactt gccgccgatg tcgtcgcaga tgtcgagcag cttctccaca
    36181 tcgccggaga ccgcggtgcc ggactcgaac ttcttcagca gctgcaccag ccaccagttg
    36241 ccctcacggc acggggtgca cttgccacag gactcgtgct tgtagaactc cacccagcgc
    36301 agggtcgtgc gcaccaccga agtggtctcg tcaaagacct gcagcgcctt ggtgcccagc
    36361 atcgagccca cgctgcccat gccttcgtag tcgagcggca cgtccaggtg ctcgggggtg
    36421 aggatcgggg tggacgagcc accgggcgtc cagaacttca gctcgtgccc agcgcgaatg
    36481 ccgccggaga gctccagcac ttgccgcagg gtgatgccca gcggcgcctc gaactggccc
    36541 ggacggttca cgtgcccgct caacgagtag atcgtcatgc ccttggactt ctcggtgccc
    36601 atggacgcga accaggccgc cccgttcgcc acgatcgccg ggacgctggc gatcgactcg
    36661 acgttgttga tcacggtcgg cgacgcgtac aggcccgcca ccgccgggaa cggcggacgt
    36721 agtcgcggct ggccacgacg gccctccagc gagtcgagca gcgcagtctc ctcgccacag
    36781 atgtaggcgc cggcgccggc gtgcacgatg atgtcgaggt cgtaaccgct gccgaggatg
    36841 ttcttgccca ggtagccggc ctcgtaggcc tcagcgaccg cggcctgcat ccggcggatc
    36901 acgtgcagca cctcaccgcg gatgtacaca aacgcggtat ggcagttgat cgcgtaggag
    36961 gcgatgatca tgccctcgac cagggtgtgc gggctggcca gcagcagcgg catgtccttg
    37021 caggtgccgg gctcggactc gtccgcgttg accaccaggt acttggggtt cgggttgtcc
    37081 ttgggcacga agctccactt catgccggtc gggaaacccg cgcccccgcg tccgcgcagg
    37141 ccggagtcct tgaccaggtt cgtgacctcg tcgggggtca gcttgagcgc cttcggcagt
    37201 cccgcgtagc cgcccgcgtc gcggtaggcg gccagcttcc aggacttgtc tgtgccccag
    37261 ttcgccgaaa ggacgggggt gagcaggtcg ctcatcaggc ctcctctgcg gccggtgccg
    37321 accaggagtt ggcctcggcg atcttcttgc ccagcagcga agcctcaccg gcgctcgggc
    37381 cttcgtccgc gcgtccgtcc gggaagccgg cgagcacccg ctcggcctcg cgccaagagg
    37441 tcagcgtggc cccgcgggac gccttgacct ccttgcccgc agccaggtct tccaggaggt
    37501 gttcggcctt gtcagggtcc atgttgtcca tgaactccca gttgaccatc atcaccgggg
    37561 cgaagtcgca cgccgcgttg cactccaggc gctccaggct gaacttcccg tccggcgtgg
    37621 tctcgtcggg ctcgataccg agcttctccg agaccttctc cagcagcgca tcgccgccca
    37681 tgaccgcgca cagcgcggtg gtgcagacgc ccacgtgatg ctcgccggcc ggacgccggc
    37741 ggtacatcgt gtagaacgtc gccaccccgg agacctgagc ggtggtgatg cccagcacct
    37801 cggcgcacgc ctcgatgccg cgcggggaga cccggccgtc gacgctctgc accaggtgca
    37861 gcatcggcag cagcgccgag cgcggctgcg ggtaacgggc cgccagttcg cgcagctcgg
    37921 ccatcgtcgt ctcgtcgatg ttggtcgagg tgtcggtcag gtcgaccgag cccgattcgg
    37981 ggaagaagga ctggaactcg tggccgctca tcggtcgacc cctcccatca ctggatcgag
    38041 cgagccgatc gcgaccacga cgtcggcgag catgccgccc tcgcactgga tcgggacgga
    38101 ctgcaggtgg ttgaagccgg ggtcgcgcag gtggcagcgg aacggcttgg tgccaccgtc
    38161 ggagaccacg tggcaggcga tctcgccctt ggggctctcg atcggcacgt acgcctggcc
    38221 ggccgggacg cggaagccct cggtgaccag cttgaagtgg tggatcagcg cctccatgga
    38281 ctcacccatg atgtgcttga tgtgctcgtg gctgttgccc tggccgtccg ggccgaccgc
    38341 gagcgtcgcc ggccaggcga tcttcgggtc gtcgatcatc accggctgac cggtggacgc
    38401 ctccagccgg tcggtggcct gcttgacgat cttcaggctc tcccacatct cgtccagccg
    38461 gacgcggaag cggccgtacg cgtccgaggt gtcccaggtg atcacgtcga agtcgtagtt
    38521 ctcgtagccg cagtagggct gctgcttgcg caggtcccag gcgtagccgg tcgagcgcag
    38581 caccgggccg gtgacaccga gcgcgaagca tgcgctcagg tcctggacgc cgatcccgca
    38641 catgcggccc ttgaagatcg ggttctcgtt gcagaacgcc gcgtactcgg gaatgtgctt
    38701 gtcgagccag gcgatcacgt cgcgcagctg ggcgatgccg gtctcgggca gatcgttcgc
    38761 cacaccaccg ggacgaacgt aggcgtggtt catccgcagg ccggtgaccg cttcgaagaa
    38821 gtcgaggatc atctcccgct cacggaagcc gatcgtcatc acggtgaggg cgccgatctc
    38881 catgccgccg gtgccgatcg cgaccaggtg ggacgcgatt cggttcagct ccatcatcag
    38941 cacgcgaatg tcgcttgcgc gctcggggat ctggtcctcg atgcccagca gccgctcgac
    39001 cgccaggcag taggcgacct cgttgaaaat cggggccacg tagtccatgc gggtcacgaa
    39061 cgtggtgccc tgggtccagg accggtactc catgttcttc tcga
//
LOCUS       DAOPRQ010000014        38866 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_803_length_38866_cov_11.891397, whole genome shotgun sequence.
ACCESSION   DAOPRQ010000014 DAOPRQ010000000
VERSION     DAOPRQ010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 38866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38866
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_803_length_38866_cov_11.891397"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            410..2071
                     /locus_tag="PKJ61_02955"
     CDS             410..2071
                     /locus_tag="PKJ61_02955"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020386550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HOC12817.1"
                     /translation="MGTSTQTKHIFVTGGVVSSLGKGLTASSLGTLLVARGLRVTMQK
                     LDPYLNVDPGTMNPFQHGEVFVTEDGAETDLDIGHYERFLNVCLNAEANVTTGKVYSR
                     VIAKERRGDFLGDTVQVIPHITNEIKDEMLAMAGADVDVVIHEIGGTVGDIESLPFLE
                     AARQVRREVGRENAFFLHVSLVPYIGPSGELKTKPTQHSVAALRQVGIQPDAIVCRAS
                     MEIPTGVKRKIALMCDVDEEAVISCPDAPSIYDIPKVIFREGLDAYVVRRLGVSFRDV
                     NWSSWDDLLKRVHSPKEEVTIALVGKYIDLPDAYLSVSEALRAGGFANWAKVNLRWVA
                     SDSCETPAGAAAELGDVDGICVPGGFGIRGVEGKLGALRYARENQVPTLGLCLGLQCM
                     VIEAARNLLGLTDAASTEFEPDTHAPVIATMAEQVDIVSGQGDMGATMRLGAYPAELV
                     PGSIVAKQYGSTHVSERHRHRYEVNNHYRTELEAEGLKISGTSPDGNLVEFVELDADV
                     HPYYVGTQAHPELKSRPTQPHPLFVGLVQAALDRKLSARLPVTEG"
     gene            2074..2763
                     /locus_tag="PKJ61_02960"
     CDS             2074..2763
                     /locus_tag="PKJ61_02960"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOC12818.1"
                     /translation="MRELSRAEVTDIPMTWPVVGHEVLGNGAVSDFVNDVVATPDGAT
                     MRRQYLLHPGAVGVIAWDDADRIAVVRQYRHPVGYQLVEPPAGLLDGDGETWLTAAKR
                     ELAEEVQLRAERWQVLVDLFTTPGANQESLRIYLATGLSPEPAPADFAAEHEEADMEV
                     CWAARADVVDAVLAGRLQNPTMVSGVLALEVARLSGRLDQLRDAASDWPARTAWAAHN
                     SSLAGRDGHSG"
     gene            2972..3886
                     /locus_tag="PKJ61_02965"
     CDS             2972..3886
                     /locus_tag="PKJ61_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HOC12819.1"
                     /translation="MVRTYLAHLSVERGASVHTVAAYRRDLGRYLEFLGARGINDPTT
                     ITEADVAAFLAYLQAPTDGREPLSRSSATRSLVAVRSLHAFALADGWVPENVAVGVRP
                     PKGTRPLPKALDIASVEALLATPDLTTAAGLRDAALLELLYGTGARISEVLDLDVDDA
                     TGLLADPDAGLRVFGKGRKERIVPVGSYARNAVAAWLVRGRAEFAQASVRPSPALLLN
                     RRGQRLSRQSAWAVLQRAAASAKLEVEVSPHTLRHSFATHLLDGGADVRVVQELLGHA
                     SVTTTQIYTLVTAEHLREVFMAAHPRAR"
     gene            3939..4841
                     /locus_tag="PKJ61_02970"
     CDS             3939..4841
                     /locus_tag="PKJ61_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016667815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HOC12820.1"
                     /translation="MSETDSPALIDAEPTLDGGDLGPTGRPWPALPEPTPPDPNHPKR
                     ALIIAMCNQKGGVGKTTTTINLGAALAETGRRVLLVDFDPQGSLSVGLGVNPHTLETS
                     IYDLLLNKKISPEEVITQTSVPGLELMPSNIDLSAAEVQLVSEVAREQTLRRVLTKVR
                     HDYDIILIDCAPSLGLLTVNALTASDKVLMPLECEFFALRGIALLTDTIEKVQDRLNP
                     ELEVLGILGTMYDARTLHSREVLERVVQAFGDTVFHTVIRRTIKFPETTVAGEPITTY
                     ATTSPGAAAYRTLAREVLARCPGA"
     gene            4826..5158
                     /locus_tag="PKJ61_02975"
     CDS             4826..5158
                     /locus_tag="PKJ61_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC12821.1"
                     /translation="MPRRVKLPGASELFRPTSAAGVGAETPAADASAEANGPSPSGRV
                     RHEEKITVYVSSEELLALEQARLTLRAHHGLAIDRGRIVRTAIGLAMADLASRGAESD
                     LVRELGQP"
     gene            5155..5988
                     /locus_tag="PKJ61_02980"
     CDS             5155..5988
                     /locus_tag="PKJ61_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012922684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HOC12822.1"
                     /translation="MTPLPAAEEFTVHLTNFSGPFDLLLQLIAKHKLDVTEVALSKVT
                     DEFVAHIRAAQLSQGASSWDLDQTSSFIVVAATLLDLKAARLLPQGEVDDEEDLALLE
                     ARDLLFARLLQYRAFKLVSGWIAETLTLQARRYPRPGGLEERFAGLLPDVELTGFTDL
                     IAAYAGRALTPREAPQVQLTHLHLPLVSVREQADEIVSRLREHSTLTFRALTADATDR
                     LVVVARFLALLELFRDRAVTFEQLTPLGELTVRWLGAELDPERTGSEFDETQPQESTD
                     E"
     gene            5981..6628
                     /gene="scpB"
                     /locus_tag="PKJ61_02985"
     CDS             5981..6628
                     /gene="scpB"
                     /locus_tag="PKJ61_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HOC12823.1"
                     /translation="MSDLSGAIEALLLMATEPMPTAELAEAVQAPVEAVETALAGLVE
                     FYDTTGRGFELRRVGSGWRYYTREEHADLIGRWLTEGQRGTLSQAALETLAVVAYLQP
                     ISRGRVSAVRGVNVDGVMRTLATRDLIEETGRDGETGARLFATTSYFLERMGLTSLDE
                     LPPLAPHLPDASALEAELANLADPGAAVEEALLTVIPAQAGISDPNDGPLEEGSE"
     gene            6625..7362
                     /locus_tag="PKJ61_02990"
     CDS             6625..7362
                     /locus_tag="PKJ61_02990"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002524162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOC12824.1"
                     /translation="MTETEGIRLQKVLAQAGVASRRAAEDLIFRGRVEVNGEKVTEQG
                     RRVDPATDVIRVDGLRIPPKAEHLYLVLNKPTGVVSTMEDPRGRPALQELVSVYTRER
                     VFHVGRLDADTEGLLLLTNDGEFSQRMMHPSFEVPKVYVAEIAGVITEATVKRLRKGV
                     ALEDGPVQPDKVEVLQRGADRSLVRITLHEGRNRIVRRLFDEVGHPVRQLSRIAIGPV
                     RLGDLKLGAVRDLTREELGALLDLVKL"
     gene            7374..8717
                     /locus_tag="PKJ61_02995"
     CDS             7374..8717
                     /locus_tag="PKJ61_02995"
                     /inference="COORDINATES: protein motif:HMM:NF015119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome accessory factor PafA2 family
                     protein"
                     /protein_id="HOC12825.1"
                     /translation="MERVLGVETEYGLSARSARGRLSAPEAAELLFRPLVAEHRAANA
                     FLVNGGRLYLDVGAHPEYATPECRTVRQLLVAERAGDELVAQLAADASARAAAASQEV
                     RFRLFKNNVDSFGNSYGCHENYLVEREQDLSVLVDLLTPFLVTRQVLCGSGRYRRGEF
                     TISQRADHLYDQLSAQTTRARPLVNTRDEPLADPARFRRLHVLAGDSNLCEPSAWLKV
                     GTTVALLDALSGGSGVDRLGRLRLADPAAALRQVARDPSAVLELADGRRRSALEVQRE
                     YLDVAGERAEPELAGLWTAVLDALTAGAPERVADVVEWVAKRRLLQEFQDRHRLASDD
                     PKLAQLDLAFHELGGAFGALEGEGLVRRLTTSAEVRRAMTEPPADTRAAARAGFLAAV
                     QRGRHRYSVDWQTCTIHDLTGADGPADRTIALPDPLAARVPELDELLELLCANAG"
     gene            8794..9786
                     /locus_tag="PKJ61_03000"
     CDS             8794..9786
                     /locus_tag="PKJ61_03000"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOC12826.1"
                     /translation="MSRTRLIPVIASLLALAACSAPAPSATPSASATPSTSASASASP
                     SATPTSTVKASNSLDGIKVTGERLKKPSVSVKAPFAIDQTRMKVLEAGTGARALADGY
                     VTVHYYGVNGRTGKMFEESYTENMPKTFALNGVVAGFKTGLTGQQSGSRVLIAMPGTD
                     AYDASPSSGPEGYEAGDTLIFVVDVLSVSVAEPSGTTITPPAGLPTVSAGTGKPTITI
                     PSTAAPTKMVAQTLIEGTENKVAKGDSILARYVGYSWKTGKLVDDGFDSPSGGALSST
                     IPGWQTGLVGKPVGSRVLLVLPPADGFPKGSNNPPLEAGDTIVYVVDLLFATAS"
     gene            9783..10637
                     /gene="mmuM"
                     /locus_tag="PKJ61_03005"
     CDS             9783..10637
                     /gene="mmuM"
                     /locus_tag="PKJ61_03005"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008363009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047150 - betaine-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase"
                     /protein_id="HOC12827.1"
                     /translation="MIILDGGLSTVLEQQGADLSSALWTARLLADAPDRLAEAHRTYF
                     AAGAQVATTASYQASVAGFAAAGFGNAEALIGDSVRIARDVRDEFGDDRLVAASVGPY
                     GAALADGSEYRGDYGVSVAALRDFHAQRLELLAAAGPDLFAVETVPDVREAEVLLELL
                     AEFDLPAWFSYSVRGDRTSAGQRLADAYGALASCPVLVAAGVNCSAPEDVPAAVATAV
                     AVTGLPGIAYPNGGGSWDAAAKEWRRPDAWRPELVESWLAAGATWVGGCCGVGAAEIA
                     ELATLLAD"
     gene            complement(10807..11721)
                     /gene="gluQRS"
                     /locus_tag="PKJ61_03010"
     CDS             complement(10807..11721)
                     /gene="gluQRS"
                     /locus_tag="PKJ61_03010"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HOC12828.1"
                     /translation="MHPRDLEEAGLSGRFAPSPTSALHLGNLRTALVAWLLARTTGRE
                     FRLRIEDLDVARVAAAPEVAAQQVDDLLALGLDFDGEVIWQSARVDAYAEAVGRLETY
                     ECFCTRREIAEAASAPHGDGYRPYPGTCRALSAAARERLRQTRPPALRVRAAEDSSTV
                     TDLWAGEVTGVVDDFVLVRNDGTYAYNLAVVVDDLAMGIDQVCRGDDLLSSSPRQAWL
                     AQQLGGPAPVYAHVPLAVNASGKRLAKRDGAVTLADLAVTGVTPAEALSRIAASLELA
                     TPGEPVTPEQLLIRFDPDELPRQPWLVA"
     gene            11799..12719
                     /locus_tag="PKJ61_03015"
     CDS             11799..12719
                     /locus_tag="PKJ61_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013161239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HOC12829.1"
                     /translation="MNLAICLLMARRFIEKTQIREVVEGYHDLSDAAFERTFERDKDE
                     LRAMGVPVETGSNNPLFPDDVGYRIRRKDFELPAIEFTPAETAALGLAATVWESATQA
                     EHAISALAKLRAAGVDPDPQRLAGLAPSIGAREPAFASIWAATGARTPIRFDYRGQTR
                     TVEPWAMTYRRGAWYLYGRDRAKGEPRMFKVARLDAPAVPAGKPASYTVPELDLEAAW
                     RSLEPSRADASAIVAIKPGRAADLRRRATPTEGVYDVPSGYEPLRVSYSRVGDFVGEL
                     CAYGADALVLKPLYIRRAVIAQLAAVAGGK"
     gene            12719..13669
                     /locus_tag="PKJ61_03020"
     CDS             12719..13669
                     /locus_tag="PKJ61_03020"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HOC12830.1"
                     /translation="MTSADQVSRLLSLVPYLQTHPDAELSATAELFAVSTEQLLADLH
                     VLWYCGLPGGMPGDLIEIDMETVADTGRIRLSNAEYLSRPLRFTPDEALSLVVALRAV
                     RELVGRDAAGDVDTALVKLEAAAGAGVGAPVPRVAVSGGDEDLRQRLAEAIASGVVTE
                     LEYTDSGLVTSSPLVAPAQLIVRDGFGYLQAWSLDREAWRTYRLDRITAVRETTRKAP
                     EHGTPPDFGAGWLDERPDAAEVTLRLTPSAEWITEYYPVRAVRHPRGAVEVDLLVADP
                     AWLRSLLLRLGREVRRVSPPEAADGARAAAADALIAYAAG"
     gene            13679..13870
                     /locus_tag="PKJ61_03025"
     CDS             13679..13870
                     /locus_tag="PKJ61_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC12831.1"
                     /translation="MNWILLFGLIALGGLIMVVSYGIWLWHKASDLFSELEMLGERAT
                     ELADILGRLQPELPGRTDG"
     gene            13907..14032
                     /gene="tatA"
                     /locus_tag="PKJ61_03030"
                     /pseudo
     CDS             13907..14032
                     /gene="tatA"
                     /locus_tag="PKJ61_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015070765.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
     gene            14189..14950
                     /gene="tatC"
                     /locus_tag="PKJ61_03035"
     CDS             14189..14950
                     /gene="tatC"
                     /locus_tag="PKJ61_03035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HOC12832.1"
                     /translation="MSLADHLRELRYRVIVAAGAIIAASIFAAVFYNQLYLVLMRPYL
                     QAVEMLKESNPNLDTSAVISGVGTPLALALQICVVAGLVLSSPFWLYQLWSYIVPALL
                     AKEKKYALIFIGVALPLFLLGVLVGYYILPQGISVLLAFTPSSVPVTNLLAIDEFLKF
                     TLQLMVVFGLGFLMPVVVVGLNLMGVVSAKQLGKARTYVIFGTFLFGAAATPSTDPFS
                     MLALALPMTLLYLAAESIARINDRRKAKAQLAEAG"
     gene            14952..15908
                     /locus_tag="PKJ61_03040"
     CDS             14952..15908
                     /locus_tag="PKJ61_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HOC12833.1"
                     /translation="MTLHYNSPTLTLVVNPAAGGGLARRQLPRVTSELVAGLPEASLR
                     VIQTSSFAEARLRCIAAVEQARPAEPGRMADSLLVMGGDGMMHLGLNAAAATDVSLGL
                     IPAGTGNDFCRGVGLTGGLNGALRTIVGGVTRRIDLMSVSGALVDGAEHRWVGSILST
                     GFDSRVNYRANHQRFRFGGLAYSWAALAELNRFEPLTYRLVIDGVPRNQSAMMIGVGN
                     AGYFGGGMCGCPNADVTDGWLDVTIVHPVSRATMLRLLPTMFTGGFVKDPAVELLGAR
                     EVVVDGDGLFGMADGEEIGQVPLTCRIAPRALSIYSRVGSDG"
     gene            15901..18624
                     /locus_tag="PKJ61_03045"
     CDS             15901..18624
                     /locus_tag="PKJ61_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOC12834.1"
                     /translation="MADALQTFASGYPFALDDYQVEACTHVANGHGVLVAAPTGAGKT
                     VVGEFAVFLALQTGRKCFYTTPIKALSNQKYHDLCERHGEDAVGLLTGDTSINGDAQV
                     VVMTTEVLRNMIYAGSSTLANLGFVVMDEVHYLADRFRGAVWEEVIISLADSVQLVAL
                     SATVSNAEEFGDWLSEVRGGVEVVVTERRPVPLYQHVLAGRRLLELFDGPDVNPELVR
                     LAKEESRSQRDDSRRPRGRSGNGRRGGYGSGRYGGGAASERQEASRRLVPRRDAMVER
                     LAAENLLPAIIFIFSRQGCDTAVGQLLNSGLRLTSAAERARLREIAQRHIAGLSQGDL
                     AALEFDRFLAAFTDGIAAHHAGLLPAFKEAVEEGFSEGLVKVVFATETLALGINMPAR
                     SVVLERLVKYNGETHADVTAGEFTQLTGRAGRRGIDVEGHAVVCWQPGLDPRALAGLA
                     SRRTYPLRSSFSPTYNMAVNLVATAGRERARVLLEQSFAQFQSDRSVVGLAKGIATNR
                     ERIASEWAQAACTEGDAEEYFRLRARVGAAEAAVARARRSDRRAEVIEALGTLKTGDI
                     IDIPGRRHRGWAVVVESGLGQEGSAPTVLTADRQLKRVSVADFPYPPSVGGFLKVPKH
                     FDSRSPAMRRNLAAALLAKLQRDRPVATADADQADPDAMVRIAELRRELRAHPVHGCP
                     DREQHARYAEAALRLERDNEALQGQADRRTNTIAVRFDRVCAVLESMGYLSADGGKHV
                     TERGRMLARIYSELDLVTAEAVVDGIFDGLSAPQLAAVISALVYEARGDRRAPARMPD
                     RRSEEAQQALRNLWRQVSLAERDHRLPSHDPPDIGFAEAIHAWTAGAELADVLYLSGL
                     TAGDFVRWARMVVDAAGQLADAVGPGELRRVCREVIAGIRRGVVDAAPLED"
     gene            18759..19520
                     /gene="hisF"
                     /locus_tag="PKJ61_03050"
     CDS             18759..19520
                     /gene="hisF"
                     /locus_tag="PKJ61_03050"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016667809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HOC12835.1"
                     /translation="MTLALRVIPCLDVHDGRVVKGVNFRNLRDAGDPVELAAAYGAEG
                     ADELVFLDISASADGRATTYDVVTRTAETVFIPLCVGGGVRGVEDVDRLLRTGADKVG
                     INTGAINRPPAIDEIVRRFGDQVLVLSLDARREAGQPSGFGVTTHGGRTSAGLDAIAW
                     VREAAERGVGEILLNSMDADGTTDGFDLELIRAVKAVVDVPVIASGGAGTVEHFVEAA
                     RAGADAVLAASVFHYQTLSISQVKAGLSAAGFAVR"
     gene            19526..19924
                     /locus_tag="PKJ61_03055"
     CDS             19526..19924
                     /locus_tag="PKJ61_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HOC12836.1"
                     /translation="MSCLFCSIVAGEIPSKQIYADEAAIAFLDINPWHVGHTLVVPRR
                     HLDDLLGEPEVLSEVAPAIAATSRLLVDRLSADGLNLLSNSGVAAGQEVFHLHFHLVP
                     RFARRPGLGELLQREPGIDLDRVYRQLTEA"
     gene            complement(20000..21253)
                     /locus_tag="PKJ61_03060"
     CDS             complement(20000..21253)
                     /locus_tag="PKJ61_03060"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HOC12837.1"
                     /translation="MRTLQLPPTVRPLLSAAIGATVLGVALAGNVVPAQADEPVVPLN
                     SQVTIVGAGWGHGRGMSQYGAYGAASSGLDYTAIVGFYYPGTELSKLDAGNTVRVWLT
                     ADTDAKLHFRKVSGLQVRDDAGTTLTLPTSSKYTKWRINKSGSNRVLYYRNKSGKYVK
                     LKNKLQPAANWYVANPRTGTVKLALPSGETKSYRGSLGLAFTAAGARTVNYVAMEDYL
                     RSVVPAEMPASWATEAVKAQSVAARTYAARLRAKAAAGSGYDLCDTSACQVYKGLAWE
                     HAASDRAIAATADQVLLYQGQPAMTEFSSSNGGWSAAGGADQPYLKSQPDPYDGVKRD
                     QTWQVTVSTSKLAKLFPGIGALTSVQVLTRDGNGTYGGRVESVQLTGSAGALTVTGAD
                     FKRKLGLKERLFTVSTGTATTLARK"
     gene            21553..23340
                     /locus_tag="PKJ61_03065"
     CDS             21553..23340
                     /locus_tag="PKJ61_03065"
                     /inference="COORDINATES: protein motif:HMM:NF005860.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HOC12838.1"
                     /translation="MRRSHRTRQKGREMPPDTPPMLTGAQALVESLERVGVDVIFGIP
                     GGSILPAYDPLYDSKIRHILVRHEQGAGHAAEGYAHVTGRVGVCMATSGPGATNLVTA
                     IGDAYMDSVPMVAITGQVASRAIGTDAFQEADIRGITFPITKHSFLITDPTDIPHAIA
                     AAFHLAATGRPGPVLVDIAKDALAMSAPFVWPEELDLPGYHPVAKPHGKQIREAARLI
                     AGAQRPVLYVGGGVNRARAWAELAELVDITGIPVVTTLMARGAIPDSHELNYGMPGMH
                     GTVAAVGALQRSDLLITLGARFDDRVTGKLDSFAPEAKVIHADIDPAEISKNRLADVP
                     IVGDLAEVIRELNDTLRQTELDRPTEWVRYLDGLRDRYVVNEYEPIEDGLLSPEYVIQ
                     RIGAIAGPDATFAAGVGQHQMWAAHLLPHERPGKFLNSGGAGTMGYCVPAAMGAKVGR
                     PDDVVWGIDGDGCFQMTNQELVTCALEGIPIKIAVLNNRSLGMVRQWQTLFYKERYSN
                     TNLQSHRVPDFPKLAEAMGAVGLRAETPEEVEEVIKQAMAINDRPVVVEFVVHKDAMV
                     WPMVAAGTSNDDIKIARDLAPDWEGGEDL"
     gene            23337..23879
                     /gene="ilvN"
                     /locus_tag="PKJ61_03070"
     CDS             23337..23879
                     /gene="ilvN"
                     /locus_tag="PKJ61_03070"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HOC12839.1"
                     /translation="MNTHTLSVLVENKPGVLARIAGLFARRGYNIESLAVGPTENEEF
                     SRITVQVAVDSQLVLEQIVKQLNKLIEVLKIVELEPASSVARELMLIKVKADPATRSQ
                     IIEIVQLFRAKTVDVHNDSMTIEATGSPDKLGALLEMLKPYGVRELVQSGLVALGRGS
                     RSLSDRAKIDKTRPAVRLAN"
     gene            23898..24926
                     /gene="ilvC"
                     /locus_tag="PKJ61_03075"
     CDS             23898..24926
                     /gene="ilvC"
                     /locus_tag="PKJ61_03075"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HOC12840.1"
                     /translation="MAEMFYDADADLSIIAGRNVAVIGYGSQGHAHALSLRDSGVDVR
                     VGLREGSKSWAKAEAEGLRVVTPAQAAEEADLIMILAPDQVQRHLYKEAIEPNLVPGD
                     ALFFAHGFNIRFGYITPPEGVDVCMVAPKGPGHLVRREYSEGRGVPVIVAVEKDESGN
                     AWPLALAYAKAIGGLRAGGIKTTFTEETETDLFGEQAVLCGGMSALVQAGFEVLTEAG
                     YQPEVAYFECLHELKLIVDLMYEGGIAKQRWSVSDTAEYGDYVSGPRVIDASVKARMQ
                     EVLADIQSGAFAKRFIDDQDAGAPEFKALRAAGEAHPIEETGRKLRGLMAWVKSHDDD
                     YVEGTAAR"
     gene            25135..26052
                     /locus_tag="PKJ61_03080"
     CDS             25135..26052
                     /locus_tag="PKJ61_03080"
                     /inference="COORDINATES: protein motif:HMM:NF024734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HOC12841.1"
                     /translation="MDVIRARELVSQGKSHRALSRQAGSGALVRVRHGAYADALAAPA
                     INRHLQLVAGTWPTLGPNTVISHVSAGVLHDLPSWTGLLGRVTVTRSGSGHGERSTHL
                     YARRSDLSGQEVIELGGYRVTSLERTAIDFSCLLNYEQAVSVLDAALHAKADLALLAA
                     TVEAAGRKRGIGTARAALAFADGRSESPGESISRVRIAEVGLERPELQVNIFDEYGNW
                     VARTDFGWLLRGVVGEFDGRIKYAGTPEEVSRAVMKEKAREAAIRRLGWVVVRWGWDD
                     LANRAAFRDRIESAFTQASNQILGDARPA"
     gene            26296..28722
                     /locus_tag="PKJ61_03085"
     CDS             26296..28722
                     /locus_tag="PKJ61_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014847055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HOC12842.1"
                     /translation="MTNTATLDPGLSTAEVEDRVRRGLVNELPPRSGRSTWDIVRANV
                     FTRINFLLMVLFIMVLSTGSIVNALFGFLIIINSGVGIVQELKAKRTLDSLAVVGEGK
                     PTVRRDGVPVELHREELVLDDVIEIGPGDQIVVDGEVVEASYLEVDESLLTGESDPLE
                     KTPGSEVMSGSFVVAGFGAFRATKVGRDSYAAKLTSEASKFSLVKSELQQGINQILRL
                     ITWLLIPVGLATIWVQWGQRTDETWQQVVLRMAGALVPMVPEGLVLLTSLAFAVGVVR
                     LGKQQCLVQELPAIEGLARVDVVCADKTGTLTENGLKFTDLDTLDSNDIPGLRKVLAQ
                     LAAGDPRPNASMQALAHELPLTEQQWAITAMAPFTSAKKWSGMSFEGHGNWVIGAPDV
                     LAPAKSAAASRAEQIGAGGRRVLLLAESDRLVDALGAPGTVTPRALLVLEQKVRPEAA
                     GTLAFFAEQNVDVKVISGDNATSVGAVTGSLGVDTGEVVDARTLPEDADEFAARVEEA
                     GVFGRVTPQQKRQMVAALQSKGRNVAMTGDGVNDVLAIKDADLGVAMGSGAAATRSVA
                     QIVLLNDSFATLPHVVAEGRRVIGNIERVANFFLTKTMYSIILALLVALWRIPFPFVP
                     IHISFVGWFTIGIPASILALAPNAERARPGFLKRVMSFSIPAGIVVAVTSFTAYLLVK
                     PATPDPVLSTQASTAALVTLMMGGAWVMIATARPYQWWKLLMIGLCLLAAYGVIFWLP
                     YWLPAWAEVIPLLGNLIPQLQMDPANAAMMTTTASMGLLAIVLIEAIWWISGRVTGER
                     RHLFGTLAAD"
     gene            complement(28729..28974)
                     /locus_tag="PKJ61_03090"
     CDS             complement(28729..28974)
                     /locus_tag="PKJ61_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC12843.1"
                     /translation="MLAAEREASGDELLLRTVYAAAYHGQLTPSARPENEARANYLIA
                     LAQPATGWPAAAMGAADRCLVVCEQHGLADFAVDPVI"
     gene            complement(29058..30116)
                     /locus_tag="PKJ61_03095"
     CDS             complement(29058..30116)
                     /locus_tag="PKJ61_03095"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013161220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HOC12844.1"
                     /translation="MRTADKPVISVIAGDGIGPEVVAEGLKVLTTVVGEDAFDFVHHD
                     LGAERWQRTGEVLPDSELETLAASQAIILGAVGAAPGSKAIPSGLLERGLLLKLRFAF
                     DHYVNLRPSKLYPGVPTPLATSVTERGPIDFVVVREGTEGLYCGNGGAFRAGTDFEVA
                     TEVSINTAHGVERVIRDAFERAENRRKAVTMVHKHNVLVHAGGLWNRLFQTVAAEHPD
                     VTTDYLHVDAATIALVQDPQRFDVMVTDNLFGDILTDLAAAVTGGIGLAASANINPTG
                     AFPSMFEPVHGSAPDIAGQGIADPTATILSMALLLDHLGRPADARRIEVAVDADIAER
                     GATKRPTSVVGDEIAARI"
     gene            30187..31272
                     /locus_tag="PKJ61_03100"
     CDS             30187..31272
                     /locus_tag="PKJ61_03100"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016455677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HOC12845.1"
                     /translation="MSLTFPLHRNPNPAGDDEVARVLANPGFGDHFTDHMAVATWTKA
                     DGWAADEVKPYGPFQMDPASAVLHYGQEIFEGLKAYRHADGSIHLFRPEANAERFQSS
                     AARMMLPELPVADFVTAVEELVKVDERWVPVSAGEQSLYIRPFMFASEVFLGVRAAER
                     VTFCVIVSPAGPYFAGGVQPVDIWVTDEYSRAGVGGTGAAKCGGNYASSLIAQYEGYE
                     HGCSQVLFIEAADKDRVEELGGMNVFLITADGRLVTPALTGTILEGITRDSILTVAAE
                     LGLTPEERRITPDELFSGIADGSTVEAFSCGTAAVVTPIGAFKSKAGEWRLPENGSPQ
                     TLAIRNAILDIQYGRVPDTHGWLKRVR"
     gene            complement(31679..32179)
                     /gene="tpx"
                     /locus_tag="PKJ61_03105"
     CDS             complement(31679..32179)
                     /gene="tpx"
                     /locus_tag="PKJ61_03105"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002518107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HOC12846.1"
                     /translation="MATLVHRGLTVHSIGELPEVGSKLPGFVLTSTGMTDDLGPDVAV
                     GKRLVLNIFPSIDTGVCAMSVRRFNELAAGLPDAVVLCVSNDLPMAQARFCGAEGIDN
                     VVTGSGFRSSFGDDFGVTLLDGPMRTLLSRAVVVADADGTITYTQQVPNISEEPDYAA
                     ALAALA"
     gene            32248..33849
                     /gene="cimA"
                     /locus_tag="PKJ61_03110"
     CDS             32248..33849
                     /gene="cimA"
                     /locus_tag="PKJ61_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013161218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HOC12847.1"
                     /translation="MTQLPAAPDSFHVFDTTLRDGAQQEGLQLTVADKLKIARHLDDL
                     GVDFIEGGWPGANPNDTAFFAAAAAGELKLRNAKLVAFGATRKVGAAAADDPQVQALL
                     DANTEVICIVAKSHDEHVFRALHTTLEENLAMVTDTVAHLVANGRRVFVDCEHFFDGY
                     RANPDYALEVVRAAAEAGAEVVVLCDTNGGMLPSWMGDIVSAAAQIGVDLGIHCHNDT
                     GCAVANTLAAVEAGVMHVQGTMNGHGERTGNANLITVIANLQLKYGWQVVTPDQLAEA
                     TRVSHAISGVTNYPMNTRQPYVGASAFAHKAGLHASAIKVDANLYQHIDPKLVGNDMR
                     MLVSDMAGRANIQIKGSELGYDLSDRDLAAKVTEKVKAREMDGYSYESADASFELLLR
                     DELGLLEDYFEVLSWRVLTGHEVKPEGDSEAVVKVRAKGLTYNLVGEGHGPLNALDVA
                     LRNALNPAYPQVDEFELTDYKVRIIDQGHGTDAIVRTLIDMTDGERTWTTVGVGTNVI
                     EASWEALHDSYLWALAHPGFEGADA"
     gene            33876..34538
                     /locus_tag="PKJ61_03115"
     CDS             33876..34538
                     /locus_tag="PKJ61_03115"
                     /inference="COORDINATES: protein motif:HMM:NF019656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1707 domain-containing protein"
                     /protein_id="HOC12848.1"
                     /translation="MTSSDEIRIGHVEREQAVADLRRAAEDGRLTAEELDERAERARA
                     ARTSAELAALFADLRPGSSVTVPVAMPTAAQALAALANVGNRPEAPLILDAGWSGTKR
                     TGAWVVPPFMVARAGIETVRIYCLQARAATEVIDLVVEATMGTVVLVLPDGWAVNVDR
                     LTRGIGTVKVKVPGIPAPGCPLFLVRGSVGMGTFKARPANRLDRWRLRRQREREQRAL
                     TA"
     gene            complement(34613..35896)
                     /locus_tag="PKJ61_03120"
     CDS             complement(34613..35896)
                     /locus_tag="PKJ61_03120"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016667802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HOC12849.1"
                     /translation="MSTFDAVELIRTKRDGGRLSEPAIDWLIRAYTDGIVTDEQMAAM
                     AMAIFFRGLDADELTRWTNAMIATGERMDFSSLSRPTVDKHSTGGVGDKITLPLAPLV
                     AACGAAVPQLSGRGLGHTGGTLDKLEAIPGWRASLSNTEMLAQLESVGAVICAAGAGL
                     APADKKLYALRDVTATVESIPMISASIMSKKIAEGTSSLVLDVKTGSGAFMKSVADSR
                     ALAQTMVGIGTAAGVNTVALITDMNAPLGRTAGNGLEVAESVDVLAGGGPSDVVELTV
                     ALAREMLLCAGIDTDPADVLASGKAMDTWKAMIRAQGGDPDAPLPTARHTEVITASAD
                     GYLAGMDAMAVGLAAWRLGAGRAVQGEQVQAAAGVTWHAGVGDRVAAGAPLFTLHTDT
                     PERMERAREALADAVTYADSPVDRGPLVLERISAT"
     gene            35920..36852
                     /locus_tag="PKJ61_03125"
     CDS             35920..36852
                     /locus_tag="PKJ61_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012922463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HOC12850.1"
                     /translation="MARRIWLPYPDSAAAETALGPLPEGLVADYFLADGSWPDSIDEV
                     ELLVAPYMHPAPAVMARVGEMDRLKVVQLLSAGYDNFLPYLPDGVQLCNAAGVHDAST
                     AELAVGLALASNRHLDTYARNMPAGIWGNDFATSLADRRVLIVGYGRIGAAIERRLAG
                     FEVASVTRVARRARTGAVPVRSIGELPALLPDADVVFVITPLTPETEHLIGAAELAKL
                     ADGALLVNVSRGKVVDTEALADATASGRVRAALDVTDPEPLPPDHRLWRTPGVLISPH
                     VGGQSTAFYPRMQRLVRAQLQRFAVGEPLENVVA"
     gene            37007..37810
                     /locus_tag="PKJ61_03130"
     CDS             37007..37810
                     /locus_tag="PKJ61_03130"
                     /EC_number="3.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016455679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HOC12851.1"
                     /translation="MRIARFVAQGDPTYGVVELAEDSGPNPDTIAVVSGDPLAGPVHY
                     TGERLPLADVRLVAPVIPRSKVIGIGRNYAAHAAELGNEVPDQPIIFSKPNTSVIGPG
                     EPIVYPPESKDLHYEGELAVVIGRICRRVPVERAADVIFGYTVANDVTCRDLQKSDGQ
                     WTRAKGFDSFCPIGPWIATHLDIDEAGRLDIETTLNGEVVQSGNTELMVRGVPELIAY
                     VSAFTTLLPGDVILTGTPAGVGPMQPGDRVSVTIAGIGTLTNVVVAEDA"
     gene            37812..38753
                     /locus_tag="PKJ61_03135"
     CDS             37812..38753
                     /locus_tag="PKJ61_03135"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOC12852.1"
                     /translation="MTSAVLHEPRPGVTYPEVLLVAENESPARRIALGLGGPLAGVIT
                     FLVVTPLVTAGLIGLGWVLAGSPGDWRSFYAAAAKFEEPAGVFATHLGLSMLIPISFG
                     LVYLLHRFNPRWLHSVQPGFRWRYTFAATLVAVVVLGGLWVLSRVGEAWVFAPEKNLW
                     WFLAAIVITSPLQAAAEEYFFRGYLLQAIGMASPAGPWFGVVGSAAVFALLHGTQDVP
                     LFLHRFAFGLIAGFLVLKTGGLEAGIAAHVVNNLVAFGYGALSGTLAETKGVTTVGWV
                     EVAWNLAAFALFAAGALWVARRMRLATTTPGVRVGSV"
BASE COUNT         6000 a        13070 c        13504 g         6292 t
ORIGIN      
        1 tcccggcgag ggtcgggatc ccgcaccggt cttcccggcg agggtcggga tcccgcaccg
       61 gtcatcccgg cgagggtcgg gatcccgcac cggtcatccc ggcgaaggcc gggatctctg
      121 agtttgaaag atcgacgcgc acaacgtccg gggacgggtt cgacaagctt gaccggccga
      181 gtggaagcga gtgcgccgga tgactggtgc tggtggctca gggatcccgg ccttcgccgg
      241 gatgacgatt gctgggctcg cggatccggg cctgcgccgg gacggtgggg gttgggcacg
      301 gatcccggtc tgtggcggga tgatggtgag cggctcagga aaccaagacg gcgtcccgcg
      361 gagcgtcgac gtcgtgagca gcgtggtttc tcctatacgc tggaaccccg tgggcacctc
      421 gactcaaacc aagcacattt tcgttaccgg gggagtcgtc tcctctctgg gcaaggggct
      481 aaccgcgtcc agtctgggca ccctgctggt ggcccgcgga ctacgcgtaa ccatgcagaa
      541 gctggatccg tacctgaacg tcgatccggg caccatgaac ccgttccagc acggcgaggt
      601 cttcgtgacc gaggacggcg ccgagaccga cctcgacatc ggccactacg aacgcttctt
      661 gaacgtctgc ctgaacgccg aagccaacgt cacgaccggc aaggtgtact cgcgggtgat
      721 cgcaaaggag cgccgcggcg acttcctcgg cgacaccgtg caggtgatcc cgcacatcac
      781 caacgagatc aaggacgaga tgctggccat ggccggcgcg gacgtcgacg tggtgatcca
      841 cgagatcggc ggcactgtcg gcgacatcga gtccctgccg ttccttgagg ccgcccgcca
      901 ggtgcgtcgc gaagtcggcc gcgagaacgc cttcttcctg cacgtctccc tggtgcccta
      961 catcggcccg tccggcgaac tcaagaccaa gcccacccag cactcggtgg ccgcgctgcg
     1021 ccaggtcggc atccagccgg acgcgatcgt ctgccgcgcc agcatggaga ttcccaccgg
     1081 cgtgaagcgc aagatcgcgc tgatgtgcga cgtcgacgag gaggctgtga tctcctgccc
     1141 cgacgcgccc agcatctacg atatcccgaa ggtgatcttc cgtgagggcc tggacgccta
     1201 cgtggtgcgc cggctcggcg tctccttccg cgacgtgaac tggtcgtcct gggacgacct
     1261 cctcaagcgc gtccactcgc cgaaggaaga ggtcacgatc gcgctcgtcg gcaagtacat
     1321 cgacctgccg gacgcgtacc tgtcggtctc cgaggcgctg cgggccggcg gcttcgccaa
     1381 ctgggcgaag gtgaacctgc gctgggtcgc gtccgacagc tgtgagaccc ctgctggcgc
     1441 ggccgccgaa ctcggcgacg tggacggcat ctgcgtcccg ggtggtttcg gcatccgcgg
     1501 cgtggagggg aagctcggtg ccctgcgtta cgcccgggag aaccaggtgc ccacgctcgg
     1561 cttgtgcctc ggcctgcagt gcatggtgat cgaagccgca cgcaacctgc tcggcctcac
     1621 cgacgccgcg tccaccgagt tcgagccaga cacccacgct ccggtaatcg ccaccatggc
     1681 cgagcaggtc gacatcgtct ccggccaggg cgacatgggc gcgaccatgc ggctgggcgc
     1741 ctacccggcc gagctggtgc cgggttcgat agtggccaag cagtacggca gcacgcacgt
     1801 gagcgagcgg caccgccacc gctatgaagt gaacaaccac taccgcaccg aactggaggc
     1861 cgagggactg aagatctccg gcaccagccc ggacggcaac ctggtcgagt tcgtcgaact
     1921 ggacgccgac gtgcatccct actacgtcgg aacgcaggcg catccggagt tgaagtcgcg
     1981 gcccacccag ccacacccgc tgttcgtcgg cctggttcag gctgcgctcg accgcaagct
     2041 gtcggctcga ctcccggtca ccgagggctg aacatgcgtg agctgagccg agccgaggtc
     2101 accgacatcc cgatgacctg gccggtcgtc gggcatgagg tgctcggaaa cggcgcggtg
     2161 tccgacttcg tcaatgacgt cgtggccacc ccggacggcg ccaccatgcg gcgccagtac
     2221 ctgctgcacc cgggcgcggt cggagtgatc gcctgggacg acgccgaccg gatcgccgtg
     2281 gtccggcagt accggcaccc ggtgggctac cagctggttg agccgcctgc gggcttgctg
     2341 gacggcgacg gcgaaacctg gctgactgcg gcgaagcgcg agctggccga ggaggtgcag
     2401 ctgcgggccg agcgttggca ggtgctggtc gatctgttca ccactccggg tgccaaccag
     2461 gagtcgctgc ggatctacct ggccaccggg ctcagcccgg aaccggcgcc ggccgacttc
     2521 gccgccgagc acgaggaagc cgatatggag gtctgttggg ctgcccgcgc cgatgtggtg
     2581 gacgcagtgc tggccggccg gttgcagaac ccgaccatgg tttcgggtgt gctcgcgctg
     2641 gaggtggcgc ggctgtccgg tcgactcgac cagttgcgcg acgcggcgtc cgattggccg
     2701 gcgcggacgg cttgggccgc gcacaactcg tcgttggccg gacgcgatgg ccactccggc
     2761 tgagcccgcc cccacggtcg aacccaggcc cacgactgag cctgccccct ctgctcagcc
     2821 tggccccact gctcagcttg gccccaacgt catcccggcg gaggccggga tcaccggcca
     2881 gggtcggcac acaccggctg gggtcacccc taacgtcatc cgggcgttgg ccgggatccc
     2941 cacccgcgag caacgcagcc ttgctgatcg gctggtccgc acctaccttg cccacctgag
     3001 cgtggaacgc ggcgcgtcgg tgcacaccgt cgccgcctac cggcgcgatc tcggccgcta
     3061 cctggagttc ctcggtgccc gcggcatcaa cgatcccacg acgatcaccg aggccgatgt
     3121 agccgcgttc ctcgcctacc tgcaggcgcc gaccgacggc cgcgagccgc tgtcccgatc
     3181 gagcgctacc cgctcgctgg tcgccgtgcg ctcgttgcac gctttcgcac tggccgacgg
     3241 ctgggtgccg gagaacgtgg ccgttggtgt ccggccgccg aagggcaccc ggccgctgcc
     3301 caaggcgctg gacatcgcgt ccgtcgaagc gctgctggcc actccggatc tgaccacggc
     3361 cgccgggttg cgggacgcgg cgctgctgga gttgctttac ggcaccggtg cccggatctc
     3421 tgaagtgctc gatctcgatg tcgatgatgc gaccggccta ctggcagatc cggacgccgg
     3481 cctgcgagtg ttcggcaagg gccgcaagga gcggattgtg ccggtcggct cgtacgcccg
     3541 gaacgcggtt gcggcctggc tggtccgtgg ccgggccgag ttcgcgcagg cgtccgtccg
     3601 gcccagcccg gcgctgctgc tgaaccgccg cggccagcgg ttgagccggc agagcgcctg
     3661 ggccgtgctg cagcgcgcgg ccgcgtccgc gaagttggag gtcgaggtgt ctccgcacac
     3721 cctgcggcac tcgttcgcca cccatctgct ggacggtggc gcggacgtcc gcgtggtgca
     3781 ggagctgctg gggcacgcgt cggtgaccac cacgcagatt tacacgctgg taacggccga
     3841 gcacctgcgc gaggtgttca tggctgccca tccgcgggcg cgctagacgg cggcgagccc
     3901 ttgcagactt gtcgacattc tgggcctaga gtgtcggggt gagcgaaacc gacagccccg
     3961 ccctgatcga cgccgaacca acgctggacg gcggcgacct gggcccgacc ggacgcccgt
     4021 ggccggcgct gcccgaaccc acgccgcccg acccgaatca ccccaagcgc gcgctgatca
     4081 tcgcgatgtg caaccagaag ggcggggtcg gcaaaaccac gaccacgatc aacctcggtg
     4141 cggccctcgc cgagaccggc cgcagggtgc tgctggtcga cttcgacccg cagggttcgc
     4201 tttcggtcgg gctcggggta aatccgcaca cccttgagac cagcatctac gacctgctgc
     4261 tgaataagaa gatctcgcct gaagaggtga tcacccagac ctcggtgccc gggctcgaac
     4321 tgatgccgtc caacatcgac ctgtcggcgg ccgaggtgca gctggtctcc gaggtggcac
     4381 gcgaacagac cctgcgccgg gtgctcacca aggtgcggca cgactacgac atcatcctga
     4441 tcgactgcgc gccgtcgctg ggcctgctga cagtcaacgc cctcaccgcc tccgacaagg
     4501 tgctgatgcc gctggaatgc gagttcttcg cactgcgcgg catcgcgttg ctcaccgaca
     4561 ccatcgaaaa ggtgcaggac cgactgaacc cggaactcga agtgctgggc attctcggca
     4621 ccatgtacga cgcccgcact ctgcacagcc gcgaagtgct cgaacgggtc gtgcaggcct
     4681 tcggtgacac cgtcttccac accgtcattc ggcgcacgat caagttcccc gaaaccacgg
     4741 tggccggcga gcccatcacg acctacgcca ccacctcgcc gggcgccgcc gcctaccgga
     4801 cgctggccag ggaggtgctt gcccgatgcc caggcgcgtg aagctaccgg gggcgtccga
     4861 gctcttccgg ccaaccagtg cagccggggt gggtgctgag acgccggctg cggacgcgtc
     4921 cgcggaagcc aacgggccga gcccgagcgg ccgcgtccgc cacgaggaga agatcaccgt
     4981 ctacgtcagc agcgaggaac tgctcgcgct ggaacaggcg cggctgaccc tgcgggcgca
     5041 ccacgggctg gcgatcgacc gcggccggat cgttcgcacc gcgatcgggc tggcgatggc
     5101 cgatctggcc agccggggtg ccgaatccga tctggtgcgc gaactcggcc aaccgtgacc
     5161 ccgctgccgg cggccgagga gttcaccgtc cacctgacca acttctccgg ccccttcgac
     5221 ctgctgttgc agctgatcgc caagcacaag ctggatgtca ccgaggttgc gctctccaag
     5281 gtcaccgacg aattcgtggc ccacatccgg gccgcgcaac tcagccaggg cgcaagttcc
     5341 tgggatctcg accagaccag ttccttcatc gtcgtggcgg cgacgttgct cgatctcaag
     5401 gcggctcgcc tgctgccgca gggcgaggtg gacgacgagg aggacctcgc gctgttggag
     5461 gcccgcgacc tgctcttcgc ccggctgctg caataccgcg cgttcaagct ggtcagcggc
     5521 tggatagccg agacgctcac gctgcaggct cgccgctacc cgcgtccggg cgggctggag
     5581 gagcgcttcg ccgggctgct gccggacgtc gaactcaccg gcttcaccga cctgatcgcc
     5641 gcctacgccg gccgcgcgct caccccgcgc gaggcgccac aggtgcagct gacccacctg
     5701 cacctgccgc tggtcagcgt gcgcgaacag gccgacgaga tcgtgtcccg cctgcgtgag
     5761 cacagcactc tgaccttccg cgctttgacc gccgacgcta ccgaccggtt ggtcgtggtg
     5821 gcccgcttcc tcgcactgct cgagcttttc cgcgatcgcg cggtcacctt tgagcagttg
     5881 actccgctgg gcgagttgac cgtccgctgg ctcggcgcgg aactcgaccc cgagcgcacc
     5941 ggttccgaat tcgacgagac ccaaccacag gagagcactg atgagtgacc tgagcggtgc
     6001 gatcgaggcc ctcctcttga tggccaccga accgatgcca accgctgagc tggccgaagc
     6061 tgtgcaggct ccggtcgagg cggtcgagac cgcgctcgcc ggactggtcg agttctacga
     6121 caccaccgga cgcggcttcg agttgcgtcg ggtggggtcg ggctggcggt actacacccg
     6181 cgaggagcac gccgacctga tcggccgctg gctcaccgag ggccagcggg gcacgctcag
     6241 ccaggcggcc ctggagacgc tggccgtggt ggcctacttg cagccgatct cccgtggccg
     6301 ggtctcggcc gtgcgcggcg tgaacgtgga cggggtgatg cggacgctgg ccactcgcga
     6361 cctgatcgag gagaccggac gcgacggcga gaccggggcg cggctgttcg ccaccaccag
     6421 ctacttcctg gaacgaatgg ggctgaccag cctggacgag ctgccaccgc tggcgccgca
     6481 cctgccggac gcgtccgcac tcgaagccga actggccaat ctggccgacc cgggcgcggc
     6541 tgttgaggag gcgctgctta ccgtcatccc ggcgcaggcc gggatctccg acccgaacga
     6601 cggcccgctc gaggagggat ccgaatgacc gaaaccgagg gcatccgcct gcagaaggta
     6661 ctcgcccagg ccggtgtggc ctcgcgccgg gccgcggaag atctgatctt ccgtggccgg
     6721 gtcgaggtga acggcgagaa ggtgaccgaa cagggtcggc gcgtcgaccc cgccacggac
     6781 gtgatccggg tcgacggcct gcgcatcccg cccaaggccg agcaccttta cctggtgctc
     6841 aacaagccca ccggcgtggt gtcgaccatg gaggatccgc gcggccgtcc ggccctgcag
     6901 gagttggtca gcgtgtacac cagagagcgg gtcttccacg tcggacggct cgatgccgac
     6961 accgaaggac tactgctgct caccaacgac ggggagttct cccagcggat gatgcacccg
     7021 tccttcgagg tgcccaaggt ctacgttgct gaaatcgccg gcgtcatcac cgaggccacg
     7081 gtgaagcgcc tgcgcaaagg cgtggcgctc gaagatggcc cggtgcaacc cgacaaggtc
     7141 gaggtgcttc agcgcggcgc cgaccgcagc ctcgtgcgga taaccctgca cgagggccgc
     7201 aatcggatcg tccggcgtct tttcgacgag gtcggccacc cggtgcgaca actctcccgg
     7261 atcgccatcg gccccgtccg cctgggtgac ctgaagctcg gcgccgtgcg cgacctcacc
     7321 cgcgaggaac tgggcgcgct actcgacctg gtgaagctgt agggagccgg ggagtggagc
     7381 gcgtcctcgg ggtcgaaacc gagtacgggc tgagcgcacg ttcggcccgg ggccggctga
     7441 gcgcgccgga ggccgccgag ttgctgttcc ggccattggt ggctgagcac cgagcggcga
     7501 acgcgttcct ggtcaacggc ggccggttgt acctcgacgt gggcgcccat cccgagtacg
     7561 ccaccccgga atgccgcacg gtgcggcaac tgctggtcgc ggagcgggcc ggtgacgaac
     7621 tggtcgccca gttggccgcg gacgcctcgg cccgggccgc tgccgcaagc caggaggtgc
     7681 ggttccggct cttcaagaac aatgtcgaca gctttggcaa cagctacggc tgccacgaga
     7741 actacctggt cgagcgcgag caggacctgt ccgttctggt ggacctactg acgccgttcc
     7801 tggtcacccg ccaggtgctt tgcgggtcgg ggcgctaccg ccgcggtgaa ttcacgatca
     7861 gtcagcgggc ggaccacctg tacgaccagt tgtccgcgca gacgacgcgc gcgcgcccgc
     7921 tggtgaacac cagggacgag ccgctggccg acccggcacg cttccggcgg ctgcatgtgc
     7981 tggccggcga ctccaacctc tgcgagccgt cggcgtggct caaagtcggc actaccgtcg
     8041 cgttgctgga cgcgctgtcg ggcggctcag gggtggaccg actcggacgg cttcggctgg
     8101 ccgatccggc cgccgcgctt cggcaggtgg cgcgggatcc gtccgcggtg ctggagttgg
     8161 cggacgggcg gcgccgctct gccctggaag tgcagcgtga gtacctcgac gttgccggcg
     8221 agcgtgctga gcccgagttg gccgggctct ggacggcggt gctggacgcg ctgaccgcgg
     8281 gtgctccgga gcgggtggcc gacgtggtgg agtgggtagc caaacgacgt ctgctccagg
     8341 agttccagga ccggcaccgc ttggcgtccg atgacccgaa gctcgcccag ttggacctcg
     8401 cgttccacga actcggcggc gccttcggcg cgctcgaggg cgaggggctg gtgcgtcgcc
     8461 tcaccacatc ggccgaagtg cggcgcgcga tgaccgagcc gccggccgac accagggctg
     8521 cggccagggc cgggtttctg gcggccgtgc aacgagggcg ccaccgctac agcgtcgact
     8581 ggcagacctg caccatccac gacctcactg gtgcggacgg ccccgccgac cgcaccatcg
     8641 ccctgcccga cccgctggcc gctcgcgtgc cggagctgga cgagctactg gagctgttgt
     8701 gtgccaacgc cggctgatca ccagctttgt agccaacggg gcgccgcccc ggtgtggtca
     8761 gctcgcatag cgcggttcgg taccctagcc agcgtgtcca gaactcgcct gatcccagtc
     8821 attgccagcc tgctcgccct tgcggcgtgc agcgcgcccg caccgagtgc gacgccgtcc
     8881 gcgtctgcga cgccttcgac gtcggccagc gcgtccgcgt cgcccagcgc gacgccgact
     8941 tcgaccgtga aggcgtccaa ctcgctggac ggcatcaaag tgaccggcga gcggctcaag
     9001 aagccgagcg tgtccgtgaa ggcaccgttc gcgatcgacc agacccggat gaaggttctc
     9061 gaagcgggca cgggcgccag ggcgctcgcc gacggctatg tcaccgtcca ctactacggc
     9121 gtgaacggcc gcaccggcaa gatgttcgag gagtcctata ccgagaacat gcccaagacg
     9181 ttcgcgctca acggcgttgt ggccggcttc aagaccggcc tgaccgggca gcagtccggc
     9241 agccgggtgc tgatcgcgat gccggggacg gacgcctacg acgccagccc atccagcggc
     9301 ccggaaggct atgaggcagg cgacactctg atcttcgtcg tcgacgtcct ctcggtttcg
     9361 gtcgccgagc catccggaac gaccatcaca ccgccggcgg ggctgccgac ggtcagcgcg
     9421 ggcaccggca agccgacgat tacgatcccg agcaccgcgg cgccgaccaa gatggtcgcg
     9481 cagacgctga tcgagggcac cgagaacaag gtcgccaagg gcgactccat cctggcccgc
     9541 tacgtcggct actcgtggaa gaccggcaag ttggtcgacg acggcttcga ttcgcccagc
     9601 ggcggcgctc tctccagcac gattccaggt tggcagaccg gcctggtcgg caagccggtc
     9661 ggctcgcggg tgctcctggt gttgccgccg gcggacggtt tcccgaaggg cagcaacaac
     9721 ccgccgctgg aggccggcga caccatcgtc tacgtggtcg atctgctttt cgccacggcg
     9781 tcgtgattat cttggacggt gggctctcca ccgtcttgga gcagcagggt gccgacctca
     9841 gttcggcgtt gtggacagct cggctgctgg cggacgcgcc cgatcggctc gccgaggcgc
     9901 accggacgta tttcgcggcc ggtgcgcagg tggctacgac ggccagctac caggcgagcg
     9961 tcgccggctt cgccgccgcg ggctttggca acgcggaggc gctgatcggg gacagcgtgc
    10021 ggatcgcgcg cgatgtccgt gatgagttcg gcgatgaccg gctggtggca gcttcagtcg
    10081 gcccgtacgg cgccgcgctg gcggacggct cggaataccg cggcgactac ggcgtttccg
    10141 tcgctgccct acgcgacttc cacgcccaac ggttggaact gctggctgct gccggccccg
    10201 acctcttcgc cgtcgagacc gtgcctgacg tccgcgaggc cgaggtgttg ctcgagttgc
    10261 tggccgagtt cgatctgccg gcttggttca gctactcggt tcgcggtgac cgcacctcgg
    10321 ccgggcagcg gttggcggac gcctacggcg cgctcgcgag ctgccccgtg ctggtggcgg
    10381 ccggcgtgaa ctgctcagca cccgaagacg tcccggctgc ggtcgcgacc gccgtggcgg
    10441 tcactggact tcctgggatc gcctacccga acggcggggg cagttgggac gcggccgcca
    10501 aggaatggcg ccggccggac gcgtggcgtc cagaactggt cgaatcctgg ctggccgccg
    10561 gcgccacctg ggtcggcggc tgctgtggcg taggtgccgc cgagatcgcc gagctggcaa
    10621 ccctgctcgc cgattgactc cagccgcgcg agtccccgtc cacacgtccc cgtccattcg
    10681 tccgcgtcca ccggtcctca tccaccctcg caagctggac agttgtatcc gaagacgggt
    10741 cacaactgtc cagcttgcgc gggtggacgg gtgcttgcgc gggtggacgg gtgtcggggc
    10801 gggtgttcag gcgaccaacc agggttgccg cggcaactca tccgggtcga atctgatcaa
    10861 cagctgttcg ggggtcaccg gctcaccggg cgtcgcgagt tcgagcgagg cggcgatccg
    10921 gctcagcgcc tccgcgggcg tcacgccggt cacggcaagg tcggcgagcg tgaccgcgcc
    10981 gtcccgcttg gccaggcgct tgccggacgc gttcactgcc agcggcacat gcgcgtagac
    11041 cggtgccggg ccaccaagct gctgagccag ccaggcctgt cgaggggagg acgacaacag
    11101 gtcgtcaccg cggcagacct gatcgatgcc catggccaga tcgtcgacga ccacggccag
    11161 gttgtacgcg taggtgccgt cgttgcgcac caggacgaag tcgtccacaa cgccggtcac
    11221 ctcgcccgcc cacagatcgg tgactgtgct cgaatcctcg gcggcccgca cccgcagcgc
    11281 cggtggccgg gtctgccgca gccgctcgcg cgccgcggca ctcagcgcgc gacaggtccc
    11341 gggatagggc cggtacccgt caccgtgcgg cgcggacgcg gcttcggcga tctcccgtcg
    11401 ggtgcagaag cactcgtagg tctccagccg gccgaccgct tcggcatagg cgtccactcg
    11461 tgcggactgc cagatcacct cgccgtcgaa gtcgagcccc aacgccagca ggtcgtccac
    11521 ttgctgcgcc gcgacctcgg gcgccgccgc aacccgggcc acatcgaggt cctcgatccg
    11581 cagccggaac tcccgtccgg tggtgcgggc gagcagccag gcgaccaggg ccgtccgcag
    11641 gttgcccagg tgcagcgccg aggtcgggct gggcgcgaac cgtcccgaca atccggcctc
    11701 ctccaagtcg cgtgggtgca tgggcaagtc gatgcaccgg cgagtcttgc agatcgggta
    11761 acgtcaccgg cgtgtcgccg cggaagtcag aacgcatcat gaacctggct atctgcctgc
    11821 tcatggcccg ccggttcatc gagaagaccc agatccggga ggtcgtcgag ggctaccacg
    11881 acctctcgga cgcggccttc gagcgcacct tcgagcgcga caaggacgaa ctgcgggcga
    11941 tgggcgtgcc ggtggagacc ggcagcaaca acccgctgtt tcccgacgac gtcggctacc
    12001 ggatccgacg caaggacttc gagttgccgg ccatcgagtt cactccagcc gagaccgctg
    12061 ccctcggcct tgccgcgacc gtctgggagt ccgcaacgca ggccgagcac gctatctcgg
    12121 ccttggcgaa gcttcgggcc gcaggggtcg acccggatcc gcagcgactg gccgggctgg
    12181 caccgtccat cggggcgcgg gagccggcct tcgccagtat ctgggcggcc accggtgcac
    12241 gcacgccgat ccgcttcgac taccgcggtc agacccgcac cgtcgagccc tgggcgatga
    12301 cctaccggcg tggcgcctgg tacctgtacg gacgcgatcg cgccaagggt gaaccccgca
    12361 tgttcaaagt cgcccgtctg gacgcaccgg ccgtcccagc gggcaagccg gccagctaca
    12421 ccgttccgga gctcgacctg gaagccgctt ggcgtagcct tgagcccagc cgggcggacg
    12481 cgtccgcgat cgtcgcgatc aagcccggac gcgcggccga cctgaggcgc cgcgcgacac
    12541 cgaccgaagg cgtctatgac gtgccgagcg gctatgaacc cctccgggtc agctactccc
    12601 gggtcggcga cttcgtcggg gaactctgcg cctacggagc ggacgcgctg gtgctgaagc
    12661 cgctgtacat ccggcgggcc gtgatcgccc agctggccgc ggtcgcagga ggaaagtgat
    12721 gacgtcggcc gaccaggtat ccaggttgct ctctttggtg ccctacctgc agacccatcc
    12781 ggacgccgaa ctgagcgcca cagccgagct gttcgcagtg agcaccgagc agttgctcgc
    12841 cgacctgcac gtgctctggt actgcggcct gcctggcggc atgcccggtg acctgatcga
    12901 gatcgacatg gagaccgtgg ctgacaccgg ccgaatccgg ctcagcaatg ccgagtacct
    12961 gtcgcgtccg ctgcggttca ccccagacga ggcgttgagc ctggtcgtgg cgctgcgtgc
    13021 tgtccgcgaa ctggtcggac gagacgcagc cggcgatgtc gacactgcgc tcgtgaagct
    13081 ggaagcggcc gccggcgccg gggtgggcgc accagtgccc agggtggccg tttccggcgg
    13141 ggatgaggac ctgcgccagc gcctggccga agcgatcgcg tccggtgtgg tcaccgagct
    13201 ggagtacacc gactcaggcc tggtcacgtc cagcccgctg gtcgcgcccg cgcagttgat
    13261 cgtgcgcgac ggcttcggct acctgcaggc gtggagcctc gaccgcgaag cttggcggac
    13321 ctaccggctc gaccggatca cggcggtccg ggagaccacc cggaaggcgc ctgaacacgg
    13381 cacgccaccc gacttcggcg cgggctggct ggacgagcgc ccggacgccg cggaggtgac
    13441 cctccggctc acgcccagcg ccgaatggat caccgagtac tacccggttc gggcggttcg
    13501 gcacccgcgc ggggccgtcg aagtcgacct gctggtggcc gacccggcgt ggctgcgctc
    13561 gttgttgctg cgcctgggcc gcgaggtgcg gcgggtgtca ccgccagagg ctgcggatgg
    13621 ggcccgcgcg gctgcggcag acgcgctgat cgcctatgcc gcggggtagg gttcatccat
    13681 gaactggatc ctgctgttcg gcctgatcgc cctcggtggg ctgatcatgg tggtcagcta
    13741 tgggatctgg ctctggcaca aggcgtccga cctgttctcc gagctggaga tgctgggcga
    13801 acgcgcgacc gaactcgccg acatcctggg acgcctgcag ccggaactcc cggggcgtac
    13861 tgacggctaa ggttgctctg caaacgctta caacaaagga gtacccatgc tcggaccgtg
    13921 ggaatgggca atcatcgccc tgatcgcgct gctgatcttc ggcggcaccc gcctggctgg
    13981 catcggcaag agcgccggca aggccattcg cgagttcaag gaggagacgt ctacgcccgc
    14041 gaagcaggtt ccgggcagca ctccagagcc caccgaaccg acgaccatcg tcgactcgga
    14101 tcccggcacc agggcctgac ccaccttggc cacccgaacc tcaaggttcg cctggctgaa
    14161 accgccgcca gccgcccctg gtggggtgat gtcgctggcg gaccacctgc gtgaactgcg
    14221 ctaccgcgtc attgtggccg cgggtgcgat catcgccgcg agtatcttcg ccgcggtgtt
    14281 ctacaaccag ctctacctgg tgttgatgcg gccctatctg caggccgtgg agatgctcaa
    14341 ggaaagcaac cccaacctgg acacgtctgc cgtgatcagc ggtgtgggca ccccactggc
    14401 ccttgccctg cagatctgcg tggtggccgg actggtgctg tccagtccgt tctggctcta
    14461 ccagctgtgg tcgtacatcg tgccggcgct gctggccaaa gagaagaaat acgcgctgat
    14521 tttcatcggc gttgcgctgc cgctgttcct gctgggcgtc ctggtcgggt actacatcct
    14581 gccgcagggc atctcggtgt tgctggcctt cacgccgtcc agcgtcccgg tgaccaacct
    14641 gctggcgatc gacgagttcc tcaagttcac cctgcagctg atggtcgtct tcgggttggg
    14701 cttcctgatg ccagtggtcg tggtcgggct gaacctgatg ggggtggtgt ccgccaagca
    14761 gttgggcaag gcgcgcacct acgtgatctt cggtaccttc ctgttcgggg ccgctgcgac
    14821 accgtccacc gatccgttct cgatgctggc gctggcgctg ccgatgacgc tgctctactt
    14881 ggcggccgag tcgatcgccc ggatcaacga ccgccgaaag gcaaaggcgc agctggccga
    14941 ggcgggctga ggtgaccctc cattacaact cgccgacgct gaccctggtg gtcaacccgg
    15001 cggcgggcgg cggcctggcc cgccggcaac tacctcgtgt caccagcgag ttggtggccg
    15061 gcctgcccga ggcgagtctc cgggtgatcc agacttcgag tttcgccgag gcgcggctgc
    15121 gctgcattgc cgcggtcgag caggcccgtc cggccgagcc gggccgcatg gccgactcgc
    15181 tgctggtgat gggcggggac ggcatgatgc acctcggcct caacgcggcg gcggccaccg
    15241 acgtgtcgct cggactgatc cctgcgggca ccgggaacga cttctgccgc ggagtcggcc
    15301 tcacgggtgg cctgaacggc gcgctgcgta ccatcgttgg cggtgtcacc cggcggatcg
    15361 acctgatgtc ggttagcggc gcactggtcg atggtgccga gcaccgctgg gtcggctcga
    15421 tcctctcgac cggtttcgac agccgggtga actaccgggc gaaccaccag cgttttcggt
    15481 tcggcggcct ggcctatagc tgggcggccc tcgccgagct gaaccgcttc gagccgctga
    15541 cgtaccggct ggtgatcgac ggggtgccgc ggaaccagtc ggcgatgatg atcggggtcg
    15601 gcaatgccgg gtatttcggc ggcggcatgt gtggctgccc gaacgccgac gtgaccgacg
    15661 gctggctgga cgtgaccatc gtccatccgg tcagccgcgc gacgatgctg cgattgctgc
    15721 ccacgatgtt caccggtggt ttcgtgaagg atcccgcggt cgaactgctg ggggcacggg
    15781 aggtcgtggt cgacggcgac gggctcttcg gcatggccga cggggaggag atcggtcagg
    15841 tgccgctgac ctgccggatc gctccccgcg ccctgagcat ctactcccgg gtcggctccg
    15901 atggctgacg cgctgcagac cttcgcgtcc ggctacccat tcgcccttga cgactaccag
    15961 gtggaggcct gcacccatgt cgccaacggt cacggcgtgc tcgtggctgc accgaccggc
    16021 gcgggcaaga ccgtggtggg ggagttcgcg gtcttcctcg ccctgcagac cggacgcaag
    16081 tgcttctaca ccaccccgat caaggcgctg agcaaccaga agtaccacga cctttgtgag
    16141 cggcacggcg aggacgcggt cggactactc accggcgaca cctcgatcaa cggagacgcc
    16201 caagtggtcg tgatgaccac cgaggtgctg cgcaacatga tctacgcagg ctcatcgacg
    16261 ctggcgaacc ttggcttcgt ggtgatggac gaggtgcatt atctcgccga ccggttccgg
    16321 ggtgcggtct gggaggaagt gatcatctcg ctggcggact cggtgcagtt ggtggcgctg
    16381 tcggccacgg tcagcaacgc cgaggagttc ggcgactggc tcagcgaggt gcgcggcggc
    16441 gtcgaagtcg tcgtcaccga gcggcgtccg gtcccgctct accagcacgt gctggccgga
    16501 cgccggttgc tggaactgtt cgacggcccg gacgtgaacc ccgaactcgt gcgattggcc
    16561 aaggaagagt cccgttcgca acgcgacgac agtcgccgtc cgcgcggccg atcgggcaac
    16621 ggccggcgcg gtggctacgg ctcgggccgc tacggcggcg gcgctgcaag cgaacgccag
    16681 gaggcgagcc gccggctggt gccccgcagg gacgcgatgg tggaacggct cgccgcggag
    16741 aacctgctgc ccgcgatcat cttcatcttc tcccggcagg gctgcgatac ggccgtgggc
    16801 cagttgctca actcggggct gcggctcaca tcggcggccg agcgcgcccg gcttcgtgag
    16861 atcgcccagc ggcacatcgc ggggctgagc cagggcgatc tcgccgctct ggagttcgac
    16921 cgcttcctgg ccgccttcac cgatggcatc gcggcccatc acgccggact gctgcctgcg
    16981 ttcaaggagg cggtcgagga gggcttctcc gagggactgg tgaaggtcgt cttcgccacc
    17041 gaaacgttgg ctttgggcat caacatgccc gctcgctccg tagtacttga gcggctggtg
    17101 aagtacaacg gcgagaccca cgccgacgtg accgccggcg agttcactca gctcaccgga
    17161 cgggccggcc gtcgcggcat cgacgtcgag ggtcacgcgg tggtctgctg gcaacccggg
    17221 ctcgacccgc gcgcgcttgc cggactggct tcgcggcgta cctacccgct tcgttcgtcc
    17281 ttctcgccga cctacaacat ggcggtgaac ctggtcgcaa ccgccggccg cgagcgtgcc
    17341 cgggtgctgc tggaacagtc cttcgcgcag ttccagtccg accgctcggt ggtcggtctc
    17401 gccaagggga tcgcgacgaa ccgggagcgg atcgcgtccg agtgggcgca ggccgcctgc
    17461 accgaggggg acgccgagga gtacttccgg ctgcgcgccc gcgttggcgc cgccgaagcc
    17521 gcggtcgcgc gagcgcgccg ttcggatcgg cgcgccgagg tgatcgaagc gctggggacg
    17581 ctcaagaccg gcgacatcat cgacatcccc ggtcgacggc atcgcggctg ggccgtggtg
    17641 gtcgaatccg ggctcgggca ggaagggtcg gcgcccaccg tgctcaccgc ggatcggcag
    17701 ctcaagcggg tgtcggtggc cgacttcccg tacccgccgt cggtaggtgg cttcctgaag
    17761 gtgccgaagc acttcgactc gcgctcgccg gcgatgcgcc gcaaccttgc cgccgcgctg
    17821 ctggcgaagc tgcagcggga ccgtccggtg gcgacggcgg acgccgacca ggctgatccg
    17881 gacgccatgg tgcggatcgc ggagttgcgc cgggagttgc gggcgcaccc cgtccacggc
    17941 tgcccggatc gcgaacagca cgctcgctat gccgaagccg cgttgcggct ggagcgggac
    18001 aacgaggcgc tgcagggcca ggccgaccgc cgcaccaaca ctattgcggt gcggttcgac
    18061 cgggtgtgtg cggtgctgga atcgatgggc tatctgagcg ctgacggcgg caaacacgtc
    18121 accgagcgcg gccggatgct ggcccggatc tattccgaat tggacctggt gaccgctgag
    18181 gcagtcgtcg atggcatctt cgacgggctg agcgcgccgc agttggctgc ggtcatttcc
    18241 gccctggtct atgaggcgcg gggtgatcgg agggcaccgg cccgaatgcc ggatcgcagg
    18301 tcggaagagg cgcagcaggc gctgcggaac ctgtggcgcc aggtctcgct ggccgagcgc
    18361 gaccaccggc tgcccagcca cgacccgccg gacatcggct tcgccgaagc tattcacgcg
    18421 tggaccgcgg gtgccgagtt ggccgacgtg ttgtacctgt ccgggctgac cgccggcgac
    18481 ttcgtccgct gggcgcggat ggtggtggac gccgccggcc agctcgcgga cgcggtgggc
    18541 cccggcgaac tgcgccgggt ctgtcgagag gtgatcgccg gcatccgccg cggcgtggtg
    18601 gacgccgccc cgctcgagga ctagggggac ggttcctttc cgcgtcgcaa atcgcctctg
    18661 gcaaggcatt gttctcgtcg gggacggtac ctttccgcac cagggagatg cggccggacg
    18721 ggtccgtgcg gcgtcaccgc agcgcctagg ctgggcccat gactctggcc ttgcgcgtga
    18781 tcccctgtct ggacgtccat gatgggcgag tcgtcaaggg cgtgaacttc cggaacctcc
    18841 gggacgccgg cgacccggtc gaactcgccg ccgcctatgg cgccgagggt gcggacgagt
    18901 tggtcttcct ggacatctcc gcttcggctg atggtcgcgc gaccacttac gacgtggtca
    18961 cccggaccgc cgaaacagtg ttcattccgc tgtgcgtggg tgggggagtc cgcggtgtcg
    19021 aagacgtcga ccggctgctg cgcaccggcg cggacaaggt cgggatcaac accggcgcga
    19081 tcaaccgtcc gccggcgatc gacgagatcg tccgccgctt cggcgaccag gtgctggtgc
    19141 tgtcgctcga cgcccggcgc gaggccgggc agccgtcggg ctttggcgtg accactcatg
    19201 gcggacggac gtccgcgggc ctggacgcca tcgcctgggt tcgcgaagcc gccgagcgcg
    19261 gcgtgggcga gatcctgctg aactcgatgg acgccgacgg caccacagac ggcttcgacc
    19321 tcgaactcat tcgcgcggtc aaagctgtgg tggatgtgcc ggtgatcgcc tcggggggag
    19381 cgggcacggt cgagcacttc gtcgaagcag cccgagccgg agcggacgcg gtcctggccg
    19441 ccagcgtctt ccactaccag accctcagca tcagccaggt gaaggcgggc ctttccgcgg
    19501 ccggtttcgc cgtacgctga gccacatgag ttgcctgttc tgcagcatcg tcgccggcga
    19561 gattccttcg aagcagatct acgccgacga agcagcgatt gccttcctcg acatcaaccc
    19621 ctggcacgtc ggacacactc tggtcgtgcc gcgccgacac ctcgacgacc tgctcggtga
    19681 gccggaggtg ctcagcgagg tcgccccggc gatcgcggcc acgtcccggc tgctggtcga
    19741 ccggttgagc gcggacggcc taaacctgct cagcaactct ggcgtggccg ccggccagga
    19801 agtgttccat ctgcacttcc acctggtgcc gcggtttgcc cggcgtccgg gcctgggcga
    19861 actgctgcag cgggagccgg gcatcgatct ggaccgggtc taccgacagt tgaccgaggc
    19921 ctagctcgcg gattcccaac aaattggacg gtagctcgcc gtcacagcgc ggaaccgtcc
    19981 actttggtgc attgctcggc tacttgcgcg ccagcgtggt cgcggtgccg gtggacacgg
    20041 tgaacaaccg ttccttcagg ccgagcttgc gcttgaagtc ggcccccgtg accgtcagcg
    20101 caccggcaga accggtgagc tgaaccgact ccacgcgtcc gccgtaggtg ccgttgccgt
    20161 cgcgggtcag cacctgcacc gaggtcaatg cgccgatgcc agggaacagc ttggcgagct
    20221 tgctcgtgct gaccgtcacc tgccaggtct ggtcgcgctt gaccccgtcg tacgggtcgg
    20281 gctgggactt cagatagggc tgatcagccc cacccgccgc cgaccagcca ccgttcgaag
    20341 acgaaaactc ggtcatcgcg ggctgtccct ggtagagcag cacctgatcg gcggtggccg
    20401 cgatcgcgcg gtcgctggct gcgtgctccc aggccaggcc cttgtagacc tggcacgctg
    20461 aggtgtcgca gaggtcatag ccggagcctg cggcagcctt cgcacgcagc cgcgccgcgt
    20521 aggtgcgggc cgccaccgac tgcgccttga cggcttccgt agcccaggaa gcgggcatct
    20581 cggccggcac caccgaacgc aggtagtcct ccatcgccac gtagttgacc gtgcgggccc
    20641 ccgcagcggt gaacgccagg ccgagcgaac cgcgatatga cttggtctca ccgctcggca
    20701 gcgccagctt cacggttccg gtcctggggt tggccacgta ccagttcgcg gccggctgca
    20761 gcttgttctt cagcttcacg tacttgcccg acttgttccg gtagtacagc acccgattgc
    20821 tgccggactt gttgatccgc cacttggtgt acttcgaact cgtcggcaag gtgagcgtgg
    20881 tgccggcgtc gtcgcgcacc tgcaggccgg ataccttgcg gaagtgcagc ttcgcgtcgg
    20941 tgtcggcggt cagccagacc cggacggtgt tgcccgcgtc cagcttcgac agttcggtgc
    21001 ccggatagta gaagccgacg atcgcggtgt agtccagacc gctgctggct gccccgtagg
    21061 cgccgtactg cgacatcccg cggccatggc cccagccggc gccgacgatg gtcacctgcg
    21121 agttcagcgg cacgacgggt tcgtccgcct gcgcgggcac gacgttgccg gcgagcgcga
    21181 cccccaacac ggtcgcgccg atggcagccg acagcagcgg acggacggtc gggggcagct
    21241 ggagggtgcg catgcgggtc ccttctcgaa gttgcgggtc cgcagcatcg tcactcatcg
    21301 gcgtgtcgac ttgccagtcc gccaactcag attcgtcgca tgccccgctc aaagtcgaag
    21361 gctggggttg agcaattccg ggtcgcccac aatgtgatac gcaccggcca tttattgaca
    21421 tggcggcgtc cgaccgcgaa tcttgaggag tgcggaacac cgtcctcgta gttagccagc
    21481 gtgtcggctg agtagttact caccccgaca cgctccctcg cctgcgccca gcagcggggg
    21541 tttttttctg cactgagacg gtcacacaga acacgacaga aaggtagaga gatgccaccg
    21601 gacacaccgc ccatgctcac cggagcccaa gccctggtcg aatccctcga gcgcgtcggc
    21661 gtggatgtca tcttcggcat ccccggtggc tcgatcctgc cggcctacga cccgctctac
    21721 gactcgaaga tccggcacat cctggtgcgc cacgagcagg gcgccgggca tgccgccgag
    21781 ggctacgcgc acgtcaccgg acgggtcggc gtgtgcatgg cgacctccgg tccgggcgcg
    21841 accaacctgg tgaccgccat cggcgacgcc tacatggact cggtgccgat ggtcgcgatc
    21901 accggccagg tggccagccg cgcgattggc acggacgcct tccaggaggc cgacatccgg
    21961 ggcatcacct tcccgatcac caagcacagc ttcctgatca ccgatccgac ggacatcccg
    22021 cacgccatcg ccgcggcctt ccacctggcg gccaccggcc gtccggggcc ggtgctggtc
    22081 gacatagcca aggacgcgct ggccatgtcc gcgccgttcg tgtggcccga ggagctcgac
    22141 ctgcccggct accacccggt ggccaagccg cacggcaagc agattcgcga ggcggcgcgg
    22201 ctgatcgctg gggcccagcg tccggtgctg tacgtcggtg gcggcgtgaa ccgggcccgc
    22261 gcgtgggcgg aactggccga actggttgac atcaccggca tcccggtggt gaccacgctg
    22321 atggctcgcg gtgcgatccc ggacagccac gagctgaact acggcatgcc tggaatgcac
    22381 gggacggtgg ccgcggtcgg cgccctgcag cgctccgacc tgctgatcac cctcggcgct
    22441 cgcttcgacg accgggtgac cggaaagctc gactcgttcg cgcccgaggc caaggtcatc
    22501 cacgctgaca tcgacccggc ggagatctcg aagaatcggc tggcggacgt cccgatcgtc
    22561 ggcgacctcg ccgaggtgat ccgcgagctg aacgacacgc tgcgccagac cgagctggac
    22621 cgtccgaccg aatgggtgcg ctacctggac ggcctgcggg atcggtacgt cgtcaacgag
    22681 tacgagccga tcgaggacgg gctactcagc cccgagtacg tgatccagcg gatcggtgcg
    22741 atcgccggtc cggacgcgac tttcgcagca ggtgtgggcc agcaccagat gtgggccgca
    22801 cacctgctac cgcacgagcg tccgggaaag ttcctgaact ccggcggcgc gggcaccatg
    22861 ggctactgcg tgccggcggc gatgggcgcc aaggtcggcc gtccggacga cgtggtgtgg
    22921 ggcatcgacg gtgacggctg cttccagatg accaaccagg aactggtgac ctgcgccctg
    22981 gaaggcattc cgatcaagat cgcggtgctg aacaaccgca gcctcggcat ggtgcggcag
    23041 tggcagaccc tgttctacaa ggagcgctac tcgaacacga acctgcagag ccatcgggtg
    23101 cccgacttcc cgaagctggc cgaggccatg ggcgcggtcg gcctgcgcgc cgagaccccc
    23161 gaagaggtcg aagaggtgat caagcaggcg atggcgatca acgaccgtcc ggtcgtggtc
    23221 gaattcgtgg tgcacaagga cgcgatggtc tggccgatgg tcgcagccgg caccagcaat
    23281 gacgacatca agatcgctcg ggacctcgcg cccgattggg aagggggaga ggacctgtga
    23341 acacgcacac tctgagcgtc ctggtggaga acaagccggg tgtcctcgca cgcatcgccg
    23401 gcctcttcgc tcggcggggc tacaacatcg agtcgctcgc ggtcggcccg accgagaacg
    23461 aggagttctc ccggatcacc gtccaggtgg ccgtggacag ccagttggtg ctggagcaga
    23521 tcgtgaagca gctgaacaag ctgatcgagg tgctcaagat cgtcgagctg gaaccggcct
    23581 cctcggtcgc ccgggagctc atgctcatca aggtgaaggc tgacccggcc acccgcagcc
    23641 agatcatcga gatcgtgcag ctcttccgcg ccaagaccgt ggacgtccac aacgactcca
    23701 tgaccatcga ggcgacgggt tcgcctgaca aactgggtgc cctgttggag atgctgaagc
    23761 cgtatggcgt ccgcgaactg gtgcagtccg gcctggtcgc gcttggccgg ggcagccgct
    23821 ccctcagtga ccgtgccaag atcgacaaga ctcgtccggc cgtccggctg gccaactagt
    23881 aaggaacgaa gcgaataatg gcagagatgt tctacgacgc agacgccgac ctctcgatca
    23941 tcgccggacg caacgtcgcg gtcatcgggt acggcagcca gggccacgcg catgcgttga
    24001 gcctgcggga ttcgggtgtg gatgtgcggg tcggcctgcg ggagggctcg aagagctggg
    24061 cgaaggccga ggccgagggc ctgcgggtgg ttaccccggc gcaggcggcc gaagaggccg
    24121 acctgatcat gatcctggct cccgaccagg tgcagcgaca cctttacaag gaggcgatcg
    24181 agccgaacct ggtgccgggc gacgcactgt tcttcgcgca cggcttcaac atccggttcg
    24241 gctacatcac cccgcccgaa ggcgtggacg tgtgcatggt cgcccccaag ggccccggcc
    24301 acctggtgcg ccgcgagtac tccgagggcc gtggcgtccc ggtgatcgtc gcggtggaga
    24361 aggacgagag cggcaacgcc tggccgttgg cgctcgccta cgccaaggcg atcggcggac
    24421 tgcgtgcggg cggcatcaag accaccttca ccgaggagac cgagaccgac ctgttcggtg
    24481 agcaggccgt gctctgcggt ggcatgtccg ccctggtgca ggccggtttc gaggtgctca
    24541 ccgaggcggg ctaccagccc gaggtggcct acttcgagtg cctgcacgag ctgaagctga
    24601 tcgtcgacct gatgtacgag ggcggcatcg ccaagcagcg ctggtcggtg tcggacaccg
    24661 ccgagtacgg cgactacgtc tccggcccgc gggtgatcga cgcgtccgtc aaggcccgca
    24721 tgcaggaggt gttggccgac attcagtccg gcgccttcgc caagcggttc atcgacgacc
    24781 aggacgccgg cgcgcctgag ttcaaggcgc tgcgcgctgc cggggaggcc catccgatcg
    24841 aggagaccgg acgcaagctc cgcggcctga tggcctgggt gaagtcccac gacgacgact
    24901 acgtggaggg caccgccgct cgctgagccg cgcgcccgtc cgccgtttcg cagttaacgc
    24961 cagcgatccg gactcttgcc agggaacatt ccccggcaag agtccggatc gctggcgtct
    25021 agctgcggtg ggttgatttt ggggcggacg gggtgagctt ggctggtttg cacagggtgt
    25081 tgtcgatttg ttgcacccct ttcactggcg ttcggggtgc gctgaagtga aggcatggat
    25141 gtgatccgag cccgcgaact ggtcagccaa gggaaaagcc atcgggcgtt gtcgcgccaa
    25201 gccggctcgg gtgcgctggt gcgagtgcgt cacggtgcct atgccgatgc cctggcggca
    25261 ccagcgatca accgccatct acagctggtt gccggaacct ggcccacgct tgggcccaac
    25321 accgtgatca gccacgtcag cgccggggtg ctacacgatc tgccgtcgtg gaccggtcta
    25381 ttgggacgcg tgacggtaac tcgctccggt tcgggtcacg gtgagcggag cacgcacctc
    25441 tacgcgcgcc gctccgacct ttccggccag gaggtgatcg aactcggtgg ctaccgggtg
    25501 accagtctcg agcggacggc gatcgacttc agctgtctgc tcaactacga gcaggcggtg
    25561 tcggttctcg atgcggcact gcatgccaag gccgatctgg ctctgctggc ggcgacagtc
    25621 gaggccgccg gtcggaaacg tggcattggg acggcgcgtg cggcccttgc cttcgccgac
    25681 ggtcggtcgg agagcccggg cgagtccatc agcagggtac ggatcgccga ggttgggctt
    25741 gagcgtcccg aactgcaggt gaacatcttc gatgagtacg gcaactgggt ggcgagaacc
    25801 gacttcggtt ggctcctgcg cggcgtggtt ggcgagttcg acggccggat caagtacgcc
    25861 ggcacgcctg aggaagtgtc gcgcgcggtg atgaaggaga aggcgcgcga agctgcaatt
    25921 cggcggctcg ggtgggtcgt cgtccgctgg ggctgggacg acctggcgaa tcgcgccgcg
    25981 ttccgtgacc gcatcgagtc ggcgttcacc caagcgtcca atcaaattct cggcgacgcg
    26041 cgaccggcct agccggtgct cgtgctcgtc ccagccgtcc gccgctcagt tcccgccggc
    26101 gaaactgaca tttgccgggg aaggttcccc ggcgaacgtc agtttcgctg gcgggaactt
    26161 tgggggcacg agtgcgagca agctcgccct gcgctctcgt cgccgggtct cgctgcgctg
    26221 tggacccttc ctcctcggcg agtgcgagca agctcgccct gcgctctcgt cgtcgggtct
    26281 ggcaagctgg cgcccatgac taacacggcg acgctcgacc ccggactgtc cacggccgag
    26341 gtcgaggatc gagtgcggcg cgggctggtg aacgagctgc cgccacggtc gggccgcagc
    26401 acctgggaca tcgtccgggc caacgtgttc acccggatca acttcctgct gatggtgctg
    26461 ttcatcatgg tgctgagcac cggctcgatc gtgaacgcgc tgttcggctt cctgatcatc
    26521 atcaactccg gcgtgggcat cgtccaggag ctcaaggcga agcgcacgct ggacagcctg
    26581 gccgtggtcg gcgaaggcaa gcccaccgtc cgccgggacg gggttccggt cgaactgcac
    26641 cgcgaagagc tggtgctcga cgacgtgatc gagatcggcc cgggcgacca gatcgtcgtc
    26701 gacggcgaag tggtcgaggc gtcctatctc gaagtcgacg agtcgctgct gaccggcgag
    26761 tccgatccgc tggagaagac gccgggcagc gaagtgatgt cggggagctt cgtggtcgcc
    26821 gggttcggcg cgttccgggc gaccaaggtt ggccgcgact cctacgcggc caagctgacc
    26881 agcgaggcca gcaagttctc gctggtgaag tcggagttac agcagggcat caaccagatc
    26941 ctgcggctga tcacctggct gctgatcccg gtcgggctgg ccacgatctg ggtgcagtgg
    27001 ggccagcgca ccgacgagac ctggcagcag gtggtgctac ggatggccgg cgccctggtg
    27061 ccgatggtgc ccgagggttt ggtgctgctc acgtcgctcg cgttcgcggt gggtgtggtt
    27121 cggctcggca agcagcagtg cctggtccag gagctcccgg cgatcgaggg cctggccagg
    27181 gtcgacgtgg tctgcgccga caagaccggc accctcaccg agaacggcct caagttcacc
    27241 gacctcgaca ccctggattc caacgacatt ccgggcctca gaaaggtgct ggcccaactc
    27301 gccgccggcg atccgcgtcc gaatgcttcg atgcaggcgc tggcacacga actgccgctc
    27361 accgaacagc agtgggcgat caccgcgatg gcgccgttca cgtccgccaa gaaatggtcg
    27421 ggcatgtcgt tcgagggcca cggcaactgg gtgatcggcg ccccggacgt gctggccccg
    27481 gccaagagtg cggccgcgtc ccgggctgaa cagatcggcg cgggtggccg ccgcgtgctg
    27541 ctgctggccg agtccgatcg gctcgtggac gccctcggcg ccccgggaac ggtcaccccc
    27601 cgcgcactgc tggtgctgga gcagaaggtg cgcccggagg cggccggcac cctggccttc
    27661 ttcgctgagc agaacgtgga cgtgaaggtc atctccggcg acaacgcgac ctcagtgggc
    27721 gcggtcaccg gctcgttggg cgtcgatacc ggcgaggtcg tcgatgcccg caccctgccc
    27781 gaggacgcgg acgagttcgc cgcgcgcgtc gaggaagccg gggtgttcgg acgggtcacc
    27841 ccgcagcaga agcggcagat ggtggctgcc ctccaatcca agggacgcaa cgtcgccatg
    27901 accggcgacg gcgtcaacga tgtgctcgcg ataaaggatg ccgacctggg cgtcgcgatg
    27961 ggttcggggg ccgccgcaac ccgctcggtg gcccagatcg tgctgctgaa tgacagcttc
    28021 gccaccctgc cgcacgtggt ggccgagggc cgccgggtga tcggcaacat cgagcgggtc
    28081 gcgaacttct tcctgaccaa gacgatgtac tcgatcattc tggcgctgct ggtggcgttg
    28141 tggcggatcc cgttcccgtt cgtgccgatt cacatctcgt tcgtcggctg gttcacaatc
    28201 ggcattccgg cctccatcct tgcgctggcg ccgaacgccg agcgggcgcg tccgggcttc
    28261 ctgaagcggg tgatgagctt ttcgatcccg gcgggcatcg tggtcgccgt gaccagcttc
    28321 accgcctacc tgctggtcaa gccggccacc cctgaccccg tcctcagcac ccaggcgagc
    28381 accgcggcgc tggtcacgct gatgatgggc ggggcgtggg tgatgatcgc gaccgcgcgt
    28441 ccgtaccagt ggtggaagct gctgatgatc gggctgtgcc tgctggccgc ctacggggtg
    28501 atcttctggc tgccgtactg gctacccgcc tgggccgagg tgatcccgct gctcggcaac
    28561 ctgatcccgc agctgcagat ggatccggcc aatgccgcga tgatgaccac caccgcctcg
    28621 atgggcctgt tggcgatcgt cctgatcgaa gcaatttggt ggatctccgg gagggtcacc
    28681 ggcgagcggc ggcacctgtt cggcactctc gccgccgact gagagtctct agatcaccgg
    28741 gtcgaccgcg aagtcggcca ggccgtgctg ctcgcacacc accaggcagc gatccgccgc
    28801 ccccatagct gccgcgggcc agccggtcgc cggctgggcc agcgcgatca ggtagttcgc
    28861 ccgcgcctca ttctccggac gcgccgaagg cgtcagctgc ccgtggtacg cggcggcgta
    28921 gaccgtccgc agcagcaact catcgccact ggcctcgcgt tcggcagcca gcaacgcgtg
    28981 ggcggcgttg ttggcctatg cgccctggcg gcggtgccag tcggcatcca ccacgacctc
    29041 gcccgccgcg cccgcggtca gatgcgagcc gcgatctcgt cgccgaccac cgacgtcgga
    29101 cgcttggtcg cgccccgctc ggcgatgtcg gcgtccacgg ccacctcgat gcgcctggcg
    29161 tcggccggac ggccgaggtg gtcgagtagc agggccatcg acaagatggt ggcggtcggg
    29221 tcggcgatgc cctggccggc gatgtccggc gccgagccgt gcaccggctc gaacatggac
    29281 gggaacgcgc cggtcgggtt gatgttcgcc gaagcggcta gcccgatgcc gccggtgact
    29341 gcagcggcca gatcggtgag gatgtcgccg aagaggttgt cagtgaccat cacgtcgaag
    29401 cgctgcgggt cctgcaccag ggcgatcgtg gccgcgtcca cgtgcaggta gtcggtggtg
    29461 acgtctgggt gctcggcggc gacggtctgg aagagccggt tccacagccc gccggcgtgc
    29521 accagcacgt tgtgcttgtg caccatggtg accgccttgc ggcggttctc ggcgcgctcg
    29581 aaggcgtccc ggatcacccg ctcgacaccg tgggccgtgt tgatgctgac ctcggtggcc
    29641 acctcgaagt cggtgccggc acggaaggcg ccgccgttgc cgcagtaaag gccctcggtg
    29701 ccctcgcgca ccacgacgaa gtcgatcggc ccgcgctcgg tcaccgacgt ggccagggga
    29761 gtgggcacgc ccgggtacag cttggacggg cgcaggttca cgtagtggtc gaacgcgaag
    29821 cgcagcttga gcagcagacc ccgctccaga agcccggacg ggatcgcctt cgaacccggc
    29881 gcggctccca cggcgccgag gatgatcgcc tgcgaagcgg ccagggtttc cagttcggag
    29941 tcgggcagca cctcaccggt gcgctgccag cgctcggcgc ccaggtcgtg gtgcacgaag
    30001 tcgaaggcat cctcgccgac cacggtggtg aggaccttga ggccctcggc caccacctcg
    30061 gggccgatgc cgtcgccggc gatgacggaa atgactggct tgtcggctgt tctcacccgg
    30121 gcgaggttac tgggacgacc acgcttcgga cgtccctgtc tcaccactca gcggtagcat
    30181 gcgcacatgt ccctgacttt cccgctccat cgcaacccca atccggccgg cgacgacgag
    30241 gtcgcgcgag tcctggcgaa ccctggcttc ggtgatcact tcaccgatca catggccgtc
    30301 gcgacctgga cgaaagcgga cggttgggcc gcggatgagg tcaagcccta cgggccgttc
    30361 cagatggacc cggccagcgc cgtcctgcac tacggccagg agatcttcga gggcttgaag
    30421 gcctaccggc atgcggacgg ttcgatccac ctgttccgtc cggaggcgaa cgccgagcgt
    30481 ttccagtcct ccgccgcccg gatgatgctg cccgaactgc cagtggccga cttcgtcacc
    30541 gcggtcgaag aactggtcaa ggtggacgag cgctgggtgc cggtttcggc gggtgagcag
    30601 agcctctaca tccggccgtt catgttcgcg tccgaggtgt tcctaggcgt gcgtgcggcc
    30661 gagcgggtca ccttctgcgt gatcgtgtcg cccgccgggc cgtacttcgc cggcggcgtc
    30721 cagcccgtcg acatctgggt gaccgacgag tattcccggg ccggggtggg cggcacgggt
    30781 gcggccaagt gcggcggcaa ctacgcctcg tccctgatcg cgcagtacga aggctatgag
    30841 cacggctgct cgcaggtgct attcatcgag gccgctgaca aggaccgggt ggaggaactc
    30901 ggcggcatga acgtcttcct gatcaccgcg gacgggcggt tggtcacccc tgcgctcacc
    30961 ggcaccatcc tggagggcat cacccgcgac tcgatcctga ccgtggcggc cgagctgggc
    31021 ctcactcctg aggaacgccg gatcactccc gacgagctct tctcgggcat tgctgacggc
    31081 agcaccgtcg aggcgttctc gtgcggcacc gcggctgtgg tcaccccgat cggtgcgttc
    31141 aaatcgaagg ccggcgagtg gcgcctgccc gagaacggca gcccgcagac cctcgcgatc
    31201 cgcaacgcca tcctggacat ccagtacggc cgcgtccccg acacccacgg ctggctgaag
    31261 cgcgtccgct gagcgcacag ctcgtcgagg gccggcctac ccgagatccg tcgggtttcc
    31321 gtctgctgcg ccttgggttc atccctgtgg cgggcattca acccggtttt gaccggtttt
    31381 gagggcctca ccagcggccc ccgaaagaac aacccgactt tgactggtta tggagccgga
    31441 ttccggccgg atagccagtc aaagtcgggt tcatcgcaga cgggaccacg ggaccgccgc
    31501 cataaccggt cgaaagcggg tttatcggcg tcgtccgcgt ggatcatcga tcacattcgc
    31561 atcggggaac cggacttggc gccagtactc gctccagggg gtgtgctcgc aggtttggct
    31621 ggtcctcgac gggcggtcgt gacgctggcg gccgagttat cccgattaag agggcggcct
    31681 acgccagtgc ggccagcgcg gccgcgtagt cgggctcctc gctgatgttc ggcacctgtt
    31741 gggtgtaggt gatggtgccg tccgcgtccg ccaccacgac cgcgcgggag agcagagtgc
    31801 gcatcggccc gtccagcagg gtgacgccga agtcgtcgcc gaagctcgag cggaagccgg
    31861 acccggtcac gacgttgtcg atgccctcgg cgccgcagaa ccgggcctgg gccatcggaa
    31921 ggtcgttcga gacacagagc acgaccgcgt ccggcagccc ggcggcgagt tcattgaacc
    31981 gacggacgct catcgcgcag accccggtat cgatgctcgg gaagatgttc agcaccagcc
    32041 gcttgcccac tgccacgtcc gggccgaggt cgtcggtcat accggtgctg gtgagcacga
    32101 agccgggtag cttgctaccc acttcgggga gttcgccgat gctgtgtacg gtcaatccgc
    32161 ggtgcacgag tgtcgccatg tttgagatcg tagtctcgaa acctgaaacg ctggcgtaac
    32221 cgcggtgggg gacggcaaac tcacccggtg actcaattgc cagccgcacc ggacagcttc
    32281 cacgtctttg acaccacgct tcgcgatggt gcccagcagg agggactcca actcaccgtt
    32341 gccgacaagt tgaagatcgc ccgtcacctc gacgacctcg gcgtcgactt catcgagggc
    32401 ggctggccgg gcgccaaccc caatgacacc gccttcttcg ccgcggcggc cgccggcgag
    32461 ctgaagctgc gcaacgccaa gctggtggcc ttcggcgcca ctcgcaaggt cggtgccgcc
    32521 gcagccgacg acccgcaggt gcaggcgctg ctggacgcca acaccgaagt gatctgcatc
    32581 gtggcgaaga gccacgacga gcacgtcttc cgtgcgctgc acaccacgct ggaggagaac
    32641 ctcgccatgg tcaccgatac ggtggcccat ctcgtcgcca acggacgccg ggtgttcgtc
    32701 gactgtgagc acttcttcga cggctaccgt gccaaccctg actacgcgct cgaagtggtg
    32761 cgcgccgctg ccgaggccgg cgccgaggtc gtcgtgctct gtgacaccaa cggcgggatg
    32821 ctgccctcct ggatggggga catcgtgtcc gccgccgccc agatcggcgt cgatctcggc
    32881 atccactgcc acaacgacac cggctgtgcc gtggccaaca cgctggccgc ggtcgaggcg
    32941 ggcgtgatgc atgtccaggg caccatgaac ggccacggcg agcgcaccgg caacgcgaac
    33001 ctgatcacgg tgatcgcgaa cctgcagctc aagtacggct ggcaggtggt cactcccgac
    33061 cagttggccg aggccacccg ggtcagccac gcgatttcgg gcgtcaccaa ctacccgatg
    33121 aacacccgcc agccgtacgt gggtgcgtcc gcgttcgcgc acaaggccgg cctacacgcg
    33181 tccgcgatca aggtggacgc gaacctgtac cagcacatcg atcccaagct ggttggcaac
    33241 gacatgcgca tgctggtctc cgacatggcc gggcgcgcga atatccagat caagggctcc
    33301 gagctcggct acgacctctc cgaccgcgac ctggcggcta aggtcaccga gaaggtgaag
    33361 gcgcgcgaga tggacgggta ttcctacgag tcggcggacg cgtccttcga actgctgctg
    33421 cgcgacgaac tcggcttgct ggaggactat ttcgaggtgc tcagctggcg ggtgctgacc
    33481 gggcatgagg tgaagcccga aggtgattcc gaggctgtgg tgaaggtgcg tgcgaagggc
    33541 ttgacctaca acctggtcgg tgagggtcac ggcccgctga acgcccttga tgtcgcgctg
    33601 cggaacgcgc tgaatcctgc ctacccgcag gtcgacgagt tcgagctcac cgactacaag
    33661 gtgcggatca tcgaccaggg ccacggcacg gacgcgatcg tgcgtacctt gatcgacatg
    33721 accgacggcg agcgcacctg gacgaccgtc ggtgtcggca ccaacgtcat cgaggcatcc
    33781 tgggaggcgc ttcacgacag ctacctgtgg gcgctggccc accctggctt cgagggcgcg
    33841 gacgcgtagt cgcggcaccc cgctaggctg gccgggtgac cagtagcgat gagattcgga
    33901 tcggccacgt cgagcgtgag caggccgtgg ccgacctgcg ccgcgcggcg gaggacggcc
    33961 ggctcaccgc ggaggagctc gacgagcgag ccgagcgggc ccgggccgca cggaccagcg
    34021 ctgagcttgc ggcgctgttc gctgatctgc gtcctggttc ttcggttacc gtgccggtag
    34081 cgatgccgac cgccgcgcag gccctggccg ccttggcgaa cgtcggcaac cggccagagg
    34141 cgccgctcat cctcgacgcg ggatggtcgg gcacgaagcg caccggagcc tgggtggtgc
    34201 caccgttcat ggtcgcgcgt gctgggatcg agactgtccg gatctactgc ctccaggcgc
    34261 gggccgccac cgaggtgatc gatctggtgg ttgaggccac gatgggcacc gtcgtgttgg
    34321 tgctgcccga cggttgggcg gtgaacgtcg accggctgac ccgcggcatc ggcactgtga
    34381 aggtgaaggt gccgggcatt ccggccccgg gctgtccgct cttcctggtg cgcggctcag
    34441 tgggtatggg caccttcaag gcccggccgg cgaaccgcct cgaccgctgg cgcctgcgtc
    34501 ggcagcgcga gcgcgagcag cgcgccctga cggcgtagag caacgctgat cagcgctgga
    34561 cgtctgcctg ggtgttaggc cccgggcggc tgcaccacgg tcaccccgtc ggctaggtag
    34621 cgctgatgcg ctccaacacc agcggaccac gatccaccgg ggagtcggcg taagtgacgg
    34681 cgtccgccag cgcctcgcgg gcgcgttcca tgcgttcggg ggtgtcggtg tgcagcgtga
    34741 acagcggggc accggcggcg acccggtcgc ccactccggc gtgccaggtg acgccggcgg
    34801 cggcctgtac ctgctcgccc tgcactgcgc gtccggcccc taggcgccag gcagccagtc
    34861 cgacggccat cgcgtccatg ccagcgaggt agccgtccgc cgaagcggta atgacctcgg
    34921 tgtggcgggc cgtgggcagg ggagcgtccg gatcgccgcc ctgggcgcgg atcatggcct
    34981 tccaggtgtc catggccttg ccggacgcga gcacgtccgc ggggtcggta tcgatgccgg
    35041 cgcacagcag catctcgcgg gccagggcca cggtcagttc gaccacatcg gacggtccgc
    35101 cgccggccag cacgtccacc gactcggcga cctcgaggcc gttgccggcc gtccgtccga
    35161 gcggagcgtt catgtcggtg atcagcgcga ccgtgttcac ccccgcggca gtgccgatgc
    35221 cgaccatggt ctgagcgagc gcccgggagt cagctacgga cttcatgaaa gcgcccgagc
    35281 cggtcttgac gtccagcacc agcgaactgg tgccctcggc gatcttcttg ctcatgatcg
    35341 aggccgagat catcggaatc gactccacgg tcgcggtcac gtcgcgcagg gcgtagagct
    35401 tcttgtcggc cggggcaagg cctgcgccgg cagcgcagat caccgcaccg acggactcca
    35461 gttgcgccag catctcggtg ttgctgaggc tggcccgcca gcccggaatc gcttcgagct
    35521 tgtcgagggt gccaccggtg tggccgagac cgcgtccgct cagttgcggg acggccgcgc
    35581 cacaggccgc caccagcggg gccagcggca gggtgatctt gtcgcccaca ccgccggtgg
    35641 agtgcttgtc gacggtgggc cgggagagcg aggagaaatc catccgctcg ccggtggcga
    35701 tcatggcgtt ggtccagcgg gtcagctcgt cggcatccag gccacggaag aagatggcca
    35761 ttgccatcgc ggccatctgc tcgtcggtga cgatgccgtc ggtgtaggcg cggatcagcc
    35821 agtcgatggc gggctcgctg aggcggccgc cgtcgcgctt ggtgcggatc agttcaacag
    35881 cgtcgaaggt gctcacgcat cgcaccctag ggtagaggca tggctcgtcg catctggttg
    35941 ccctatccag actcggccgc agccgagacc gctcttgggc cgctcccgga agggcttgtc
    36001 gccgactact tcctggcgga cgggagttgg cccgactcga tcgacgaggt cgagttgctg
    36061 gtcgcgccgt acatgcatcc ggcgcctgcg gtgatggccc gggtggggga gatggatcgg
    36121 ttgaaggtgg tccagctgct cagcgcgggc tacgacaact tcctgcccta cctgccggac
    36181 ggggtgcagc tgtgtaacgc ggccggagtc cacgatgcca gcacggctga gttggcggtg
    36241 ggtctcgcgc tggccagcaa ccggcacctc gatacctatg cccgtaacat gccggccgga
    36301 atctggggca acgacttcgc cacctcgttg gccgaccggc gggtgctgat cgtcggctac
    36361 ggccggatcg gtgcggccat cgagcgccga ctcgccgggt tcgaggtcgc gtcggtgacg
    36421 cgggtggctc ggcgggcgcg gacgggagcg gttcccgtcc gctcgatcgg ggagctgcct
    36481 gcgctgctgc cggacgctga tgtcgtgttc gtgatcacgc cgctgacccc agagaccgag
    36541 cacctgatcg gagccgccga actggcgaag ctggccgacg gtgcgctgct ggtgaacgtg
    36601 tcccgcggca aggtggtcga caccgaggcg ctggcggacg cgaccgcgtc cggacgggtg
    36661 cgcgctgcgc tcgacgtcac cgaccccgag ccactgccgc ccgatcaccg cttgtggcgg
    36721 actcccggcg tgctgatcag cccgcatgtc ggcggccaga gcaccgcctt ctacccacgg
    36781 atgcagcgtc tggtgcgcgc ccagctgcag cgcttcgccg tcggcgagcc gctggagaac
    36841 gtggtggcct gaccgacgac tcaacttcgc tcccgaatgg gacctttgct cccgaagttt
    36901 cgggagcaac ggcccctcta gggagcgaag tcgggctcga gcgggcgctg gggccggacg
    36961 cccgcggacg ggctcggcgc aacctgtctg tcgtagactc cgcgccatgc gcatcgccag
    37021 attcgtcgcc cagggcgacc cgacctacgg agttgtcgaa ctagccgagg attcgggccc
    37081 caatccggac accatcgccg tggtctccgg tgatccgctg gccggaccgg tgcactacac
    37141 cggcgagcgc ctcccgctgg cggacgtccg gctggttgcc ccggtgatcc cgcgcagcaa
    37201 ggtgatcggc atcggacgca actacgcagc gcacgccgcc gaactcggca acgaggtgcc
    37261 ggaccagccg atcatcttca gcaagcccaa cacctctgtg atcggtccgg gcgagccgat
    37321 cgtctatccg cccgagtcga aggacctgca ctacgagggc gaactcgcgg tggtgatcgg
    37381 gcggatctgc cgccgcgttc cggtggagcg ggcggcggac gtgatcttcg ggtacacggt
    37441 cgcaaacgac gtcacctgcc gcgacctgca gaagtcggac gggcagtgga cgcgtgcgaa
    37501 gggtttcgac agcttctgcc cgatcggacc ctggatcgcc acccacctgg acatcgacga
    37561 agccggccgg ctcgacatcg agacgacgct caacggtgaa gtggtgcagt cgggcaacac
    37621 cgaactgatg gtgcgtggcg tgcctgaact gatcgcttac gtgtcggcgt tcaccaccct
    37681 gctgccaggt gacgtgatcc tcaccggtac gccggcaggg gtcggcccga tgcagcccgg
    37741 tgaccgggtc agtgtcacga tcgccggcat cggcacactg accaacgtcg tggtggccga
    37801 ggacgcctga ggtgacgtcc gccgtcctgc acgagccgcg tccgggggtt acctatcccg
    37861 aggtgctttt ggttgccgag aacgagtcgc cggcgcgccg gatcgccctc ggcctgggcg
    37921 ggccgctggc tggggtaatc accttcctgg tggtcactcc gctggtcacc gcgggcctga
    37981 tcgggctggg ttgggtgctg gcgggctcgc cgggcgactg gcgcagtttc tatgccgccg
    38041 cggcgaagtt cgaggagccg gcgggagtct tcgccacgca ccttggactg tcgatgctga
    38101 tcccgatcag cttcggactg gtctacctgc tgcaccggtt caacccgcgt tggctgcact
    38161 cggtgcaacc cggcttccgc tggcggtaca cgttcgcggc gacgctggtg gcggtggtgg
    38221 tgctgggcgg gctctgggtg ctgtcccggg tcggggaggc ctgggtgttc gctcccgaaa
    38281 agaacctgtg gtggttcctc gccgcgatcg tgatcacttc gccgctgcag gccgcggccg
    38341 aggagtactt cttccgcggc tacctgttgc aggcgatcgg catggcatcg ccggccggtc
    38401 cgtggttcgg cgtggtgggt tcggcggccg tgttcgcgct gctgcacggg acgcaggacg
    38461 tgccactgtt cctgcatcgc ttcgcgttcg gcctgatcgc aggcttcttg gtactgaaga
    38521 ccggcgggct ggaggcgggc atcgccgcac acgtggtgaa caacctggtg gcgttcggct
    38581 atggggcgct ttcggggacg ctggccgaaa cgaagggggt cacaacggtc ggctgggtcg
    38641 aggtggcctg gaacctggcg gctttcgcgc tgtttgctgc tggagcgctc tgggtggccc
    38701 ggcggatgcg gctcgcgacg acgactcccg gcgtccgggt gggctcagtt tgagccaagg
    38761 gcgtgccgtc cggtaaggtc gttcctcggc tggttcgcgg gaattggccg atggggtatg
    38821 gggtaattgg cagcccgccg gattctggtt ccgtcagtct aggttc
//
LOCUS       DAOPRQ010000015        38575 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_817_length_38575_cov_13.453583, whole genome shotgun sequence.
ACCESSION   DAOPRQ010000015 DAOPRQ010000000
VERSION     DAOPRQ010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 38575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38575
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_817_length_38575_cov_13.453583"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3286)
                     /gene="nifJ"
                     /locus_tag="PKJ61_03140"
     CDS             complement(<1..3286)
                     /gene="nifJ"
                     /locus_tag="PKJ61_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015069273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HOC12853.1"
                     /translation="MSRVIIDGNEAAASAAFRLNELCSIYPITPSSTMAELADEWSAQ
                     GRANVYGLVPTVVEMQSEGGAAGALHGALQGGALSTTFTASQGLLLMIPNMYKIAGEL
                     TSTVLHVAARSLAAQGLSIYGDHQDVMAVRQTGFAMLNSSSVQEAHDMAAVSQLATLI
                     SRVPFVHFFDGFRTSHEENGVELLTDAQLLSYVPEDLVRQHRRRALSPEHPYIRGTAQ
                     NPDTYFQGRESVNRYYEAVPGVVAEALAKFAEMSGRSYDLVEYYGHPEAERVTIIMGS
                     GAQTVRSTVAHLVAKGEKVGVVQLRLYRPFPTEAVLAAIPATAKVVAVLDRTKEPGAG
                     GEPLFLDVAAAIGEASAAGRRPHLPLLVGGRYGLSSKEFTPAMVVGIYAELALPSPRP
                     RFTIGINDDVTMLSLPYDPTIDLEDPQTLRAVFYGLGSDGTVGANKNTVKIIGGIENT
                     YAQGYYVYDSKKSGSRTVSHLRFGPNPIEAPYLVSQAGFIGCHHWSILERVDVLEFAR
                     PGTVLLINTPYEPGEVFGHLPMPMQQKIIDLGIELWAIDANMVARKAGMGRRTNTVLQ
                     TCFFAISNVMPRAAAIEEVKRTIRKTYSRKGADVVAKNEAAVDASVDNLHRIEVPAVV
                     VGDVGMIPPVPADAPDFVRNVTASMLLGTGDQLPVSALPVDGTYPSGTTQYEKRNISD
                     IIAEWDPEACIQCGNCAFVCPHAVLRAKYYAGSALNGAPEGFQSAPLNAAGFPGSRYT
                     LQVYAEDCTGCGLCVEACPVKPLGTGGSRKAINLGTVLERTKEKENVKFFESIPVNRR
                     SRIDFANVRGTQFLEPLFEFSGACSGCGETPYLKLLTQLFGGRATVANATGCSSIYGG
                     NLPTTPWSSNKYGRGPAWSNSLFEDNAEFGLGLRLAADLHTGTARKRLESLRPELGDE
                     VVDAILTAPQQYESELSQQRARVDALKALLDTMSAPGVEDLRSVADHLLRRSVWIVGG
                     DGWAYDIGSAGVDHVLASGRNVNVLVLDTEVYSNTGGQASKSTPMGAVAKFAAAGKPT
                     GKKDMALQATAYGNVYVARVAMGADPQQTLKAFREAEAYNGPSLILAYSHCIAHGIDM
                     RKGLDQQ"
     gene            complement(3283..4908)
                     /locus_tag="PKJ61_03145"
     CDS             complement(3283..4908)
                     /locus_tag="PKJ61_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013160108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HOC12854.1"
                     /translation="MARDMTGLPDLAVEGGRVAPVRTRMPVYVDLLPPCNNACPAGEN
                     IQEWLRLVKAGEEEKAWRELVKNNPFPAIHGRVCYHPCEAACNREELDGAVSIHSVER
                     YLGDLAIEKGWTFEKPRFSSGRRVLIIGAGPSGLSAAYQLAKMGHYVEIRDSSDVPGG
                     MMRYGIPEYRLPRDVLDAEISRLVDLGVRFVPNYTVKDLMADQAEGGFEAVFVAIGAH
                     LSKRIDIPNRDASKIVDAVSFLRDVASGERPLIGRKVAVYGGGNTAMDAARVARRLGA
                     EESIIVYRRTEEQMPAHAEEKEGAEAEGVKMNWLRTISSMEDDDLTVEIMELDESGRA
                     RGTGRFERLQADTVILALGQEADSNFLRRIPGMHFDHDVVQVDPRTLMTGVPGIFAGG
                     DAVPSERTVTIGVGHGKRAAQQIDAWLGNREAGARPKHDLADFDRLNLWYFGDARRHS
                     QPELEPEDRITDFDEIVGGLTDEQAHFEAARCLSCGNCFECDGCLGSCPEDAVIKLGK
                     GFRYRFDYDKCTGCATCYEQCPVHAIEMVEEKA"
     gene            5401..6417
                     /locus_tag="PKJ61_03150"
     CDS             5401..6417
                     /locus_tag="PKJ61_03150"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOC12855.1"
                     /translation="MSTAETPARAAWFRRLLPVEPPIGSGSVAAPGAFGSEPALARLA
                     QLEGLDPGIPEATRTRLYEPDGGAEISIVLWHGFTNAPSQFGEVAQNLRTLGYRVLVP
                     RMPYHGEADLLNRELAKLTVADLVEQVDTCVDIASGLGSKVWVLGLSAGGTMAAWAAA
                     TRPEVSRAALVAPLIAPVGFPLPVVRALVKWPAIVPKFYMWWDPRVKEKLTEGQSPYA
                     YPGFPVPGLFPFLHLSEALFDHSVAAGHELERVALVTNPGDFAIRKDVAQRFVEEVFA
                     PRARATVEAHVDGDLKWMHDFVDPWGGNGSTEQVVAIILAAFGVGEPTAGGVLVPPLV
                     TEQP"
     gene            complement(6482..6880)
                     /locus_tag="PKJ61_03155"
     CDS             complement(6482..6880)
                     /locus_tag="PKJ61_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HOC12856.1"
                     /translation="MTSTEPATPRQTRQRAEIRQAAEALATFATAQEIHDQLRHQGSG
                     VGLATVYRTLQSMAASGELDAIRTPDGQTAYRTCSPGHHHHLICRSCGRTVEVALPSI
                     EKLVGGIAAQHSFTKLDHELEFYGICAECA"
     gene            complement(6867..7853)
                     /locus_tag="PKJ61_03160"
     CDS             complement(6867..7853)
                     /locus_tag="PKJ61_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HOC12857.1"
                     /translation="MDWLALAFMQRALLAALITGVTAPAIGTYLVQRRLSLLGDGLGH
                     LAVAGVGLAILTGTAPLPLAIVICVLGSIGIELMRESGRASGDVGLAILFYGGLSGGL
                     LLAGLAGQGAGTLSAYLFGSLLTVATGDLWAIAGLGLVVLVVAVGLMPQLFTVSADEA
                     FARSIGLPVRLLNILVVSLAAVTVTVSMRTVGLLLVSALMVVPVATANQVMIGFRRGL
                     FASMGLGAIAAVTGTVGSFYLNTAPGSLIVVIAIVLFVLSWPLARALQRRNRPIIEVT
                     DDLAPVSHDLTEPHPHVHNEQCGHQPVQHAGHVDYIHDGHRHAAHGDHYDEH"
     gene            complement(7853..8656)
                     /locus_tag="PKJ61_03165"
     CDS             complement(7853..8656)
                     /locus_tag="PKJ61_03165"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOC12858.1"
                     /translation="MTSDRPPVIAADSVDVVLHGVPIIRGASLTVQPGELVAIMGNNG
                     SGKTTLLRALLGLVPHRGSIELFGTPLNTFQDWRSIGYVPQRSSINVQQATVREVVAS
                     GRLGRRRPFVPETHADRTAINAGLEEVGLIDRAKNPFVRLSGGQQQRVLIARAIATGA
                     DLMLWDEPLAGVDQATQQLLAKVAGNLKAAGRTTVMVLHELGAFRPLIDRSIVLVDGR
                     VMPPGYRHHEHDHDHSDHDHDDHDHRAAHGGHEVHELPRPRTHRTGLEA"
     gene            complement(8653..9588)
                     /locus_tag="PKJ61_03170"
     CDS             complement(8653..9588)
                     /locus_tag="PKJ61_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HOC12859.1"
                     /translation="MFVRTSIALLFASVLALSGCASAPSDTGVADGKLQVAVAFYPFQ
                     LVAERIGGSQVTVTNLTPPGSEPHDLELAPRQVAGLAEADLVVLQSGFQPAVDNAVTQ
                     VTPKRTVDTATFLTFLTADADDHSAADHAEDSHNHGADDPHTWLDPTNLVVVAEHVRD
                     ALSEAKPEAKAEFTANTEALTKDLIALDEDFKTGLTKCERNTFITSHAAFGYLAHRYG
                     LTQVGIRGLEPDVEPSAARIAEVQQIARTEGVTTIFFETLVSPVVSRSIAGDLGLAVD
                     VLDPLEGISADSRGNDYLEVMRANLAALRTANQCS"
     gene            complement(9640..11199)
                     /gene="cydC"
                     /locus_tag="PKJ61_03175"
     CDS             complement(9640..11199)
                     /gene="cydC"
                     /locus_tag="PKJ61_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004810295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055051 - ATP-binding cassette (ABC)
                     transporter complex, integrated substrate binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033230 - ABC-type cysteine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0033228 - cysteine export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydC"
                     /protein_id="HOC12860.1"
                     /translation="MLKRLFAAVPGARWRLSLATILAFAATAASVALMGVSAWLLSFA
                     AEHPPVMYLGAAAVGVRFFGISRGAFRYVERLVGHDVALRLQSALRLETYVRLARTTL
                     LGRRRGDLLSRVIADVEAIQDLVVRVWIPFVSSAAVLLVTSAGIAFFSPGSAGVLLLT
                     SLVAAAVVPWLSRTASARADAIALPLRGQLADAARELSRTASDLVAYGAAEDRLADFA
                     AIDEQLRLASARSAWIRGVGAAAQLLAAGTAVIGALVIAGNQVHAGTLSPVFLAVAVL
                     TPLALHEALATLILSAQTSTRARAALDRVSEVLDAEPVGSGDLADPGAPVPEPSLTVR
                     GLTAGWPGSAPVVAGLSFSVRAGEKVALVGPSGIGKTTVAATVLGLIPALAGAVEVRG
                     RIGYLAQDAHIFTTTVEENVKIGNRDATRGDVVSALGRAGLELDPARPVGETGESLSG
                     GEARRVALSRLLVGNYQVLLLDEPTEHLDAATANALIEDIWADSTDSPVLVITHDPDL
                     VARCDRVVRLG"
     assembly_gap    11255..11354
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11355..13089)
                     /gene="cydD"
                     /locus_tag="PKJ61_03180"
     CDS             complement(<11355..13089)
                     /gene="cydD"
                     /locus_tag="PKJ61_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015069283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055051 - ATP-binding cassette (ABC)
                     transporter complex, integrated substrate binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033230 - ABC-type cysteine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0033228 - cysteine export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydD"
                     /protein_id="HOC12861.1"
                     /translation="MAGPIDPRLIRRARATRGYLVAGVAVGSATAILTLGQAWLLSRS
                     VADIFATRNLSTLSWAVGGLVLVFGLKAALAWANEWLAQRASAKVKSQLRRDILGARI
                     ERPLESTASGGLVTLITQGLDGLDGYFAKYLPQLLLAVTVPLIIGVAILSADLTSAII
                     LAVTLPLIPMFMALVGWTTEAITKRRWAVQTRLANHFADLIAGLPTLQVFGRAKAQAQ
                     GLLKSETAHRNETMRTLRVSFLSALVLELLATLSVAVIAVTIGFRVVYGQLDLATALF
                     VLVLAPEAYLPVRQVGVHYHDAADGVAAVEQAFALIDGPEPGSTVIPAQAGISERHVG
                     PSATSDAALLSVRELTHTYPGTDTPALAPVSFDIQPGEIVALAGPSGGGKTTLLNAVM
                     GFLAPTGGSVTIGGEPIGRTSGWRDHIAYVGQEPGLVSGTVADNVRLGFPHATHLAVT
                     DALRRAGATELPPDQVVGDDAEGVSAGERRRIAVARALLRVTLGRAELLVLDEPTAGL
                     DADTEAGLLHSLRALGVAALVVSHRPAVLAEADRVVQIAPAIEPAAVTSAPLALNTAD
                     ASALTPQETVHL"
     gene            complement(13089..14159)
                     /gene="cydB"
                     /locus_tag="PKJ61_03185"
     CDS             complement(13089..14159)
                     /gene="cydB"
                     /locus_tag="PKJ61_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013160098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HOC12862.1"
                     /translation="MIALAIEPTVLQTLWFILIAVLWIGYLVLEGFDYGVAMLIPFLG
                     RNDKEKRVIVNTIGPVWDGNQVWLLTAGGATFAAFPGWYATLFSGLYLPLFLVLVGLI
                     IRGIAFEYRSKHPEAKWRNSFDWMASIGSFIVALVFGVGFANFIIGLPVAPDTTSSFL
                     YVVTPSPYFWQLFSWFGLLGGVTLVALFLFHGSLYLALKTKGEVHDRARAFAEKAGFV
                     AIGLGAVFLLAQQFVHPTGVLGWGLLVLSAAGLLAAWWFNKQDRNGLAFISTAVTILF
                     VAIGIFLTMWPGLGFKDAAGAVDSPLNVVTAASTEGTLTVMTVAAVIFVPIVLAYTSW
                     TYWVFRRRLGVENMPDEVVAAA"
     gene            complement(14171..15667)
                     /locus_tag="PKJ61_03190"
     CDS             complement(14171..15667)
                     /locus_tag="PKJ61_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016666757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HOC12863.1"
                     /translation="MSAETLARWQFGITTVYHFFFVPITIALSMLVAIMQTIWVRTGD
                     EKMLRLTKFYGKLFLINFAMGLVTGIVQEFQFGMNWSEYSRFVGDIFGAPLALEALLA
                     FFLESVFIGLWIFGWDRLPKKVHLATIWLASVGTMISAIFILAANSWMQNPVGATYNP
                     ITDRAEMTSLLDVLLNPVALVTLPHILSTAYMTAGGLVLGIAGWHLAKLAKVANPSEK
                     QQKDISAYTWATKFGAWVLLGAGVATIITGDIQGKIMTEVQPMKMAAAEALWETEQPA
                     AFSLLTIGTLDGSREVFSIKIPMVLSFLATGTFDGKVEGINPLAKEYAAGRLISPDNR
                     LQQEAAERLTASGLLANPSDWVPNIPVTYWTFRIMMGLGFLAMAIGAFVLWTLRKGLP
                     KDSKLWTSLMWTAPLAPLFGISFGWIFTEMGRQPFIVNGVLPVAAAESPGVSAWEVAT
                     TMVLYTVIYGALAVVAIALFMHYLKLGLPDVVEPEVLTDDDAPLTFAY"
     gene            15725..16042
                     /locus_tag="PKJ61_03195"
     CDS             15725..16042
                     /locus_tag="PKJ61_03195"
                     /inference="COORDINATES: protein motif:HMM:NF015898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HOC12864.1"
                     /translation="MAVTGELEQAVMDILWSRPSSRSVREVHELLSTDRDLAYTTVMT
                     VLDRLAKKGLLLRNLEGRAWLYAPARSRVEEATAEVLELLQRLTAAERSQLLARLSAV
                     TPA"
     gene            complement(16164..16532)
                     /locus_tag="PKJ61_03200"
     CDS             complement(16164..16532)
                     /locus_tag="PKJ61_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC12865.1"
                     /translation="MTLLTYLRADLAKLMKRRDKVALHAVRDAISAIENAETSYVATP
                     ATIGVPNEFVAGAVRFGHAERVVTDLAPQQMMALAREQVELRLAEAANYRTAGRVDQA
                     LTLKAEALALADRLDAFAAE"
     gene            complement(16572..17666)
                     /locus_tag="PKJ61_03205"
     CDS             complement(16572..17666)
                     /locus_tag="PKJ61_03205"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOC12866.1"
                     /translation="MARTWRIGEVAERTGLTRRTLRHYDDLGLLSASERSWGDYRLYT
                     EADLVRLLQIQNLKALGLSLAEIADALGDPDLDASATLASHLEHLEERIADEQSLASR
                     LRSLVDTPERSWEDVLGVIALTSRLAHPDPIIRLRAALTAAPRSTTELLDALANETDP
                     AVQEILIWALTQQPDAAAGVLGRWPAADARLRALLVRALGKLGGPEVLPTLSEALAAS
                     DPEVVTSAVRALASLPAGQAAAAGVPLAALLGSSLVPSADLLDALVQVGPAALEPMAA
                     ALQSLDPATRQLAAEAIGRLGGGLSTELGAQSSTLLSSLLHDLDASVRMAALLALNEL
                     GEAGRDAILAVTEDPQLGAVARKLLDLHVT"
     gene            complement(17824..19773)
                     /locus_tag="PKJ61_03210"
     CDS             complement(17824..19773)
                     /locus_tag="PKJ61_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOC12867.1"
                     /translation="MTTTPEPTTVSTSAPEPAVAVEAPPEAPTKANERPKYGPQRGGG
                     PMGHGPGGGSGEKAVSFGPSMKRLLGHLRPEIAMIIGVIVMTIFATIGNAVGPVILGQ
                     ATDLIFSAHVLNQTAIDFGAVGRVLLLALALYVMFFLLSWASGWMINGIVQRSVYRMR
                     QEVSQKLARLPLRYFDGQPRGELLSRVTNDVDNIAQTLQQTLAQILSNLFTLIGVVVM
                     MFVVSPILAVISLITIPIAMALTVMIGKRSQKLFAQTWKSTGELNSQIEEAYTGHDLV
                     KVFGRRREVQAQFNKKNDELYSAAFGAQFISGIIMPVMFFIGNLNYVVIAVVGGLMVA
                     TGGLNIGAVQAFIQYSRMFTHPITQLASMANLLQSGVASAERVFEVLDAEEEPADASG
                     KLSEVVTGRVEFEHVDFSYTPERPLITDLNLVAEPGHTVAIVGPTGAGKTTLVNLILR
                     FYDLNAGRITLDGVDITSVPRHDLRDQIGMVLQDTWLFGGTIRDNIAYGRRGATEEEI
                     VAAAKATYVDRFVRSLPDGYDTVINDEASNLSAGEKQLITIARAFLSDPSLLILDEAT
                     SSVDTRTELLVQRAMGKLRADRTSFVIAHRLSTIRDADVILVMEDGAIVEHGSHTELL
                     AAKGHYYRLYQSQFAAAMVDEDEAA"
     gene            complement(19770..21503)
                     /locus_tag="PKJ61_03215"
     CDS             complement(19770..21503)
                     /locus_tag="PKJ61_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOC12868.1"
                     /translation="MFRLLRRFLRPYVWPFVAVLLLQLLQAMAALFLPSLNASIIDEG
                     VATGNTAIIWTLGGVMLAVSLVQIAGQIGATWFGARAAMGFGRDVRSAIFDRALSFSA
                     KEVNHFGAPTLITRNTNDVQQVQMVVLMTATMIVSAPLMMIGGIVMALREDVGLSWLI
                     VVAVAVLAAIIGVLVSQMTPLFKSMQIRIDTLNRVLREQISGLRVIRAFVREPTESER
                     FDRANHDLATTATAVGRRMMTMFPSVFFVMNLSSVAVVWFGAFRIAEGHLQVGQLTAY
                     LAYLAQILMSVMMATMMLMIGPRAAVSAGRINEVLDTSSSVVPAEHPVTELARHGEVE
                     FRDVSFAYPGAESPVLSDISFVVRPGETTAIIGSTGSGKSTMVNLIPRLFDATEGNVS
                     VDGVDVRELDPDLLWSRIGLVPQKPYLFSGTVASNLRLGDPEATDEQLWHALEVAQAR
                     DFVEKMPDGLDSRIAQGGTNVSGGQRQRLAIARALVRRPEIFVFDDSFSALDVATDAA
                     LRAALRPETERAAVVVVAQRVSTIREAEQIIVLDDGRIVGVGAHDELLDACPTYAEIV
                     ESQFSSTEAAA"
     gene            complement(21504..22187)
                     /locus_tag="PKJ61_03220"
     CDS             complement(21504..22187)
                     /locus_tag="PKJ61_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020386180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOC12869.1"
                     /translation="MSRATPMAPEVRRQAIIDATLPLLLAQGTEVSTREIASAAGVAE
                     GTIFRVFETKQELIHATIHAALAPDAAMARLTGLPASQPVEARVAAVVEILRSEIERT
                     RSLFAHLFHPASTSATGPDTLRRAQGSLAEAPGSSGRAQGSLDGHRPPWPPPPPMHAK
                     DSRAQLTAAVAAALEPYADQFAVPTVFAAQVLSALSFATSFSLSNDHPLRQPEELADT
                     VLHGIARRG"
     gene            22572..24536
                     /locus_tag="PKJ61_03225"
     CDS             22572..24536
                     /locus_tag="PKJ61_03225"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOC12870.1"
                     /translation="MRAHHLPLLTTVSAPTVHSDYAVVAVTRPDFAADAYVGQLWRVP
                     VDGRPARRITRGFVDTSPKLSPDGSLLAFLRGSAEAPPQLALVPADGGEPMVITDAEL
                     GVREFAFSPDGKRLAFIAQVPEPGRYGTVKDVGPGREDPRHITTVQFQLNGVGYTVDR
                     RAHVFVVDVPDPYGEPPVKPIGNAAKADVDFVAVPVATQLSSGDFDHHAPTWDGDTVI
                     VVAARHETRDGDLREDLYRFESPDAAPVRLTAAESTVGVIGSPVVVGDEMFFIGGDLG
                     ASGLDFFGHNPGVFAIPRSGGSARRLTAADSVHVDGQLVADGDGVLAVNLVRGVGRAI
                     RVAASGMVELDGSMAELDEASVLAIGAAAGRRMAVVASADSPGELVDLARPDVPLTDF
                     AAALRAASAPLRPVELEAKSADGYPVHGWVFAPPTSGPHPVLLMIHGGPFSAYGPTFF
                     DEAQVLAEAGYAVLMCNPRGSASYGEAHAAAIKHDFGNLDAADVLAFLDHALATVSGL
                     DADRVGIMGGSYGGYLTAWLIANEHRFAAAIVERAYLDPKTFVGASDIGWYFPAAIHG
                     EDVRSPLLVVDQVRTPTLVIHSENDLRCPLAVALRYYTELKQRGVDAELLVFPGENHE
                     LSRSGSPLHRKARFEHILRWWDRHLPVVSA"
     gene            24597..26108
                     /gene="lysS"
                     /locus_tag="PKJ61_03230"
     CDS             24597..26108
                     /gene="lysS"
                     /locus_tag="PKJ61_03230"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007400900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOC12871.1"
                     /translation="MTEPTSANPSLSEQEQVRHDKRARLLAEGVAPYPIEVPRTHTAA
                     QVHAQWSHLVAGDETADVVSVSGRVVFIRNTGKLCFATIQDGFTQADSGVRLQVMLSL
                     AEVGQERLDAWKADVDLGDFVSVTGRVISSKRGELSVLATAWTLASKALRPLPTLHKE
                     LGEETRVRQRYADLVARPEARDMVRTRALVTRSIRETLHADGYLEIETPVLQLVHGGA
                     AARPFQTHLNAFDIDMTLRIALELFLKRAMVGGADRVYEIGRIFRNEGIDSSHSAEFT
                     MLEAYQAWGDQKSIADLTQQMIVNAADAALGTRVLDTPAGEIDLDGEWAWKPVYPTLS
                     EALGVEITPETDASVLLRIAAERGVEHDPAWPAQKLVMELFGELVEPSLLQPTFVCDY
                     PPIAQPLARPHRSDPNLIEAWDLIIGGVERGTGFSELIDPVIQRERLVAQSLKAAAGD
                     PEAMQLDEDFLAALEFGAPPMGGMGLGMDRLMMLFTGAGIRETILFPLLKPHA"
     gene            complement(26112..26411)
                     /locus_tag="PKJ61_03235"
     CDS             complement(26112..26411)
                     /locus_tag="PKJ61_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002512897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CrgA"
                     /protein_id="HOC12872.1"
                     /translation="MPESKVRKAAAEKKKLTDRAQIAEKRADEQRHAVAPGSRQWVAP
                     TFITVGLIGVLWLVVYYVTSATNIVVPLLTGLAGWNVLIGMGLMAAAFALSTLWK"
     gene            26492..27127
                     /locus_tag="PKJ61_03240"
     CDS             26492..27127
                     /locus_tag="PKJ61_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012917745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HOC12873.1"
                     /translation="MARILVVDNYDSFVYNLVQYLAQIGAEVEVWRNDDPRFASAGWA
                     DGFDGILLSPGPGTPEEAGVCIDVVREQGGKLPIFGVCLGLQSISVAFGGVVDRAPEL
                     LHGKTSLVHHDGRGVFSGLPNPFTATRYHSLALDPETVPDALDVTATTDSGVIMAVRH
                     KTQPIECVQFHPESVLTEGGYQMLANWLVACGDADAPSRAVGLTPLMALRP"
     gene            complement(27352..29112)
                     /gene="pknB"
                     /locus_tag="PKJ61_03245"
     CDS             complement(27352..29112)
                     /gene="pknB"
                     /locus_tag="PKJ61_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012917746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HOC12874.1"
                     /translation="MQPTIDEPRLLGGRYQLGPVLGRGGMAEVRRARDLRLGRDVAIK
                     QLRVDLASDPTFQARFRREAQAAAGLNHPNIVAVYDTGEESDPGTGVMVPFIVMELVE
                     GHTLREILRTGRQIMPNKALEFTQGVLEALSYSHKAGIVHRDIKPANVMLTPNGQVKV
                     MDFGIARAVADTSATMTQTAAVIGTAQYLSPEQARGEHVDSRSDIYSAGCLLYELLVG
                     RPPFQGDSPVSVAYQHVREAPVPPSQLDPEITPAMDAITLKALAKDPAARYQTAAAMR
                     ADIGRLLAGQEVTARVPVPVPPVPANASDATTVLATEDTRTAARAYEPPPRRRRWGLV
                     AFLVTLIVAAAGMGIYYLVNANRVETVEIPWVVDQLQASAEQQLISKQLQPKVVNEPG
                     PDDTTVGRVVDQSPKNGRVAIGTEVIIHVNKGPAKAVIPAVKGLNQEVATKLLADNGF
                     ASVVVEEDRDAPNTAKQGEVTGVTPAEGAKVALDTEVVLRVASGTTTMPPFRNMTREE
                     AINTANSTGFTKEIVFVEQESNAIPGTVIAQDPMAGTTVKYTQKITLTLAKAITPTTP
                     PPTTPTETASIAPTDVEPTE"
     gene            complement(29173..30591)
                     /locus_tag="PKJ61_03250"
     CDS             complement(29173..30591)
                     /locus_tag="PKJ61_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002512900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOC12875.1"
                     /translation="MNRPIRGVAVAAMIMFAALLVNVSYLYVGQQGYLSERPENRRVT
                     DARFAQDRGPIMVGNTPVAASKPVDDRYKFQRSYSNGKLYAPVTGFYSYLYRTSDLES
                     SRSAELSGQDDSQFLQRLINMAAGSTVPGGTVETTLDAKAQKAAWDALDGRKGAVVAI
                     DVKTGAVKALVSYPSYDPNDLATHDLDASTAAWKRLNADPDKPMANRATAEIYPPGST
                     FKLVTAAAALAEGMSPESKLDASSFRLPGSTRVISGNCGGSEVSLTQALMVSCNPGFA
                     RLGAELGDAKLRAQAESFGFGSKFLTDLRTAASTFPDELDGAQTAMSAIGEFEVAASP
                     LQMAMVAAAIANDGVVMEPYIVNRVLDRELKVTTQTSPQKHSVALSAENAKALQEMTV
                     AVVERGTGGRAQVPGVTVGGKTGTAHSDNERRPYAWFTGWADDPNVAVCAFVEDAEIP
                     ATDIAGGAVAAPIVKAVIEALR"
     gene            complement(30588..32024)
                     /locus_tag="PKJ61_03255"
     CDS             complement(30588..32024)
                     /locus_tag="PKJ61_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013866110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOC12876.1"
                     /translation="MDAVISYRKRRGVELFMLVFAIGLGIGGYLLTHLNRDAELPTNW
                     PWAVTILGAIGLVLHIIIRWRIPYADPLILPMVMLLNGLGLAMIHRLDLASSPPMHSA
                     ELQLMWLVIAVAAGAAVIIFLRDYRLLNRYPYLLFLAGITLLMLPLLPLIGQEKNGSQ
                     IWIRLGSYSFQPAELAKLVLTIAFAAYLVDKRDVLALAGRRVLGIELPRPRDLGPILL
                     MWLASLAVLVFQKDLGTSLLFFGLFVLMLYVATEKPSWPILGGLLFASGAYGAFLAFS
                     HVQVRVSAWLNPFADFDRNYQVIQAQFGLAWGGAFGSGWGLGRPSLIPLAKTDFIAAA
                     LGEELGVAGLSAIVLVYGIIVARGLRASLVGKEPFGKLLAAGLSFVFAIQVFAIIGGV
                     TRLLPLTGLTTPFMSQGGSSLVANWVMISLLLVITHQVRSPASQVVTEPEQWLEHETT
                     QLIPVGASPVPTADDEATQNIPTTGASA"
     gene            complement(32024..33406)
                     /locus_tag="PKJ61_03260"
     CDS             complement(32024..33406)
                     /locus_tag="PKJ61_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002517157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HOC12877.1"
                     /translation="MSLALRYLAHSEIGLVRKNNQDSAYVSPTMLVVADGMGGAAAGD
                     LASAVAIRELKAADGDHSGPEMLEVIAAAIDSAGDAMSELIEADPMLDGMGSTVCGLM
                     FDGAQFGMANIGDSRAYRYRDGDLARITRDHSWVQTLVDEGRITEAEALEHPHRSLIL
                     KVINGQPQHLPDLELVDARAGDRLLICSDGLCGLVTDAAISAHLDGPREQVIADLVDL
                     AHEAGGHDNITIIIADVVEGEPDGATQVLGAAANLDLDTLETTAEVPMLLDHPAAPDP
                     GAAERLRYSPVAKRRASTWVKVVLAVLIPLVAIGGGGALWYGYTQQQFYLGAASEHVA
                     IFQGIPEPVFNLPLSHVVHTDDTRVADLPPYYHERVLETMPAASLTDAHATLDTLRAK
                     AEQCIAARLARQTATAVPSTSATQSGIPSGSADGTADPSGSAKPTDSASPSAAPTPTE
                     SATPPAPMEC"
     gene            complement(33406..33882)
                     /locus_tag="PKJ61_03265"
     CDS             complement(33406..33882)
                     /locus_tag="PKJ61_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013866108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOC12878.1"
                     /translation="MSDLVVLALKLGFIALLWLFILFTGNVIRTDLFGRKVSAAELAP
                     PAPPGAPAPPKLSRRQAKKLPKSLVVTHGRQAGLTLPLGDVLLIGRSPDCQLVLDDDY
                     VSTRHAQITRTASGYQVEDLGSTNGSLVNNQRVTTPTAFGIGDTLRIGRTLMSVEK"
     gene            complement(33875..34588)
                     /locus_tag="PKJ61_03270"
     CDS             complement(33875..34588)
                     /locus_tag="PKJ61_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013161798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3662 and FHA domain-containing protein"
                     /protein_id="HOC12879.1"
                     /translation="MGVFDRVEKKLESAVSGAFARAFKGDVQPVELTARLQRELDAEA
                     RLLSRDRKLVPNDFRIGFSQHDHDRLTPYSKTLNSEIIPQLRSYAADAGYIFNGPVTI
                     DYYLDSTLPVGRFTVTSSAVAGVDATEDATPSEIRAANLVVEVNGVRHPLTPPGVTIG
                     RGTDADLRINDPGVSRLHARINVNGRGTDQRLDIEDLGSTNGITVDGQRTRHASLALG
                     SRIEIGTTRLAIVSPVSDV"
     gene            35092..35174
                     /locus_tag="PKJ61_03275"
     tRNA            35092..35174
                     /locus_tag="PKJ61_03275"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35125..35127,aa:Leu,seq:cag)
     gene            complement(35694..36530)
                     /locus_tag="PKJ61_03280"
     CDS             complement(35694..36530)
                     /locus_tag="PKJ61_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC12880.1"
                     /translation="MLDLTRVPDDFLEPAASVVEKVLATSQSLAPRDVMLVGAWCRNI
                     LHRALGHDFETTATRDLDLALALASWDAYRTLATTFPAVGHTGIRYRVTGIDVDLLPF
                     GEIEDPDGIVEPPTREVPFSVWAFAEILTASLPLALAPTLEVRIPTVAGFTAAKLGAW
                     LDRSAYHETKDAADLALTLHWYAENRDVQDRLYDTAEGNAILIDEQADVQRAAANLLG
                     VDVAGLIGPQRLSELLARWPGDAALLTRSLQVRGAPGWPSEFGRRQELVGALSRGLNA
                     ST"
     gene            complement(36505..37455)
                     /locus_tag="PKJ61_03285"
     CDS             complement(36505..37455)
                     /locus_tag="PKJ61_03285"
                     /inference="COORDINATES: protein motif:HMM:NF021469.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin"
                     /protein_id="HOC12881.1"
                     /translation="MNEAAAKPYTRLLSEFGIQSRLPDEGWADAYLMRGRSEQQYTVF
                     VRASFTLADAAWIGKERGPALVLSNFVSPRTADALRRAGVQYLDTAGNAWITFGDVLI
                     DVRGRPRPKGVAPAAATGNLFSTARAQVIFALLAWPDLWQAPLRELARAADVSVGQAH
                     NARQLLSEAGYRAGSRPAGQSDLLELWAAAYPTGLGAKLTRATYSGSIEHVRPAAEPD
                     TLYISGESAVPELRPATLTLYTPREDSLLPVVNRWRSDGPTNIEVRRAFWSAPDGTRP
                     GVQLAPWPLVYADLAASDDPRVRQAAQDWRQSHAGPDTSS"
     gene            38001..>38575
                     /locus_tag="PKJ61_03290"
     CDS             38001..>38575
                     /locus_tag="PKJ61_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020391168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOC12882.1"
                     /translation="MSIISIIINQAMSIYRSGVLVIDYLTTLVATVAGDVWNTLVHNW
                     PYLLASVVVASLVQVYVSADHLATWLRRRTWVAILGAVALGALTPFCSCGTTAIVLGA
                     MASSVPWAPIVAFLVSSPLTSPEEYVLSVGLFGPAFATTFFVAAIAAGLIGGGASWWL
                     ESRGLLAGQARMAAPGSASSCGGACEQQPGDG"
BASE COUNT         6128 a        12588 c        13389 g         6370 t
ORIGIN      
        1 actgctggtc gagtcccttg cgcatgtcga tgccgtgggc gatgcagtgg ctgtacgcga
       61 ggatcaggct tggcccgttg taggcctccg cctcgcggaa cgccttcagc gtctgctgcg
      121 ggtcggcgcc catcgccacc cgggcgacgt acacgttgcc gtaggcagtc gcctgcagcg
      181 ccatgtcctt cttcccggtc ggcttgccgg ccgcggcgaa cttcgccacc gcccccatcg
      241 gggtcgactt ggacgcctgg ccgccggtgt tcgagtacac ctcggtgtcc agcaccagca
      301 cgttcacgtt ccgtccggac gcgagcacat ggtcgacgcc ggccgagccg atgtcgtacg
      361 cccagccgtc accgcccacg atccacaccg agcgacgcag cagatggtcg gccaccgagc
      421 gcagatcctc gacccccggc gccgacatgg tgtcgagcag cgccttcaac gcgtccaccc
      481 gggcccgctg ctggctcagc tccgattcgt actgctgcgg cgcggtcagg atcgcgtcca
      541 ccacctcgtc gcccagctcc ggacgcagcg actccagccg cttccgcgcg gtcccggtgt
      601 gcaggtcggc ggccagccgc aggcccagcc cgaactcggc gttgtcctcg aacagcgaat
      661 tggaccacgc cggcccgcgg ccgtacttgt tggacgacca gggcgtcgtg ggcaggttgc
      721 cgccgtagat ggacgagcac ccggtcgcgt tcgccacggt cgcgcggccg ccgaacagct
      781 gggtgagcag cttcaggtac ggcgtctcgc cacagccgga gcacgcaccg gagaactcga
      841 acagcggctc aaggaactgg gtgccgcgga cgttggcgaa gtcgatccgg gagcgccggt
      901 tcaccgggat ggattcgaag aacttcacgt tctccttctc cttggtgcgc tccagcactg
      961 tgcccagatt gatcgccttg cgggaaccgc cggtgcccag cggcttcacc gggcacgcct
     1021 cgacgcacag gccgcaaccg gtgcagtcct cggcgtagac ctgcagcgtg tagcgcgaac
     1081 cggggaagcc ggcggcgttc agcggtgccg actggaagcc ctccggtgcg ccgttgagcg
     1141 cagagcccgc gtagtacttg gcgcgcagca cggcgtgcgg gcagacgaac gcgcagttac
     1201 cgcactggat gcaggcttcc ggatcccact cggcgatgat gtcggagatg ttccgcttct
     1261 cgtactgcgt cgtgccggag gggtaggtgc cgtccaccgg cagcgcgctg accggcagct
     1321 ggtcacccgt cccgagcagc atggacgcgg tgacgttgcg cacgaagtcg ggtgcgtcgg
     1381 ccgggaccgg cggaatcatc ccgacgtcgc cgacgaccac ggccggcacc tcgatgcgat
     1441 gcaggttgtc cacggacgcg tccactgcgg cctcgttctt ggccaccacg tcggcgccct
     1501 tgcgggagta ggtcttgcgg atcgtccgct tcacctcttc gatcgcggcc gcacgcggca
     1561 tcacgttcga gatcgcgaag aagcaggtct gcagcacggt gttggtgcgc cggcccatgc
     1621 cggccttgcg cgcgaccata ttggcgtcga tcgcccagag ttcgatgccc agatcgatga
     1681 tcttctgctg catcggcatg ggcaggtggc caaagacctc gcccggctcg tacggggtgt
     1741 tgatcagcag cacggttccc gggcgcgcga actcgagcac gtccacccgc tccaggatcg
     1801 accagtggtg gcagccgatg aacccggcct ggctgaccag gtagggcgcc tcgatcgggt
     1861 tcgggccgaa gcgcaggtgc gaaaccgtcc gcgaacccga cttcttcgag tcgtagacgt
     1921 agtagccctg ggcgtaggtg ttctcgatgc cgccgatgat cttgacggtg ttcttgttgg
     1981 cgccgacggt gccgtccgaa cccaggccgt agaagaccgc gcgcagggtc tgcggatcct
     2041 ccaggtcgat ggtcgggtcg tagggcagcg acagcatggt cacgtcgtcg ttgatgccga
     2101 tcgtgaaccg cggccgcggg gacggcagcg ccagttcggc gtagatgccc accaccatgg
     2161 ccggggtgaa ttccttgctg gacaggccat agcggccgcc gaccagcagc ggcaggtgcg
     2221 gacgcctacc ggcagcgctg gcttcgccaa tcgcggcggc cacgtcgagg aacagcggct
     2281 cgccaccggc gcccggctcc ttggtgcggt cgagcaccgc gaccaccttc gcggtggccg
     2341 gaatcgcggc cagcacggcc tcggtcggga acggccggta gagccgcaac tgcacgacgc
     2401 cgaccttctc gcccttggcg acgagatggg ccacggtgct gcggacggtc tgcgcgcccg
     2461 agcccatgat gatggtcacc cgctcggcct cgggatggcc gtagtactcg accaggtcgt
     2521 agctgcgtcc gctcatctcg gcgaacttgg ccagggcttc ggcgaccacg ccgggcacgg
     2581 cctcgtagta gcggttcacg gactcgcgtc cctggaagta ggtgtccggg ttctgcgcgg
     2641 tgccgcggat gtaggggtgc tcgggggaca gtgcgcgccg ccggtgctgg cgcacgaggt
     2701 cctcgggcac gtagctgagc agctgggcgt cggtgagcaa ctcgacgccg ttctcctcat
     2761 ggctggtgcg gaagccgtcg aagaagtgga cgaagggcac ccgcgagatc aacgtggcca
     2821 gctgcgagac ggcggccatg tcgtgggcct cctggacgct ggagctgttc agcatcgcga
     2881 agccggtctg gcggacggcc atcacgtcct ggtggtcgcc gtagatgctc aggccctggg
     2941 ctgccagcga ccgggccgcg acatgcagca cggtcgaggt gagctcgccc gcgatcttgt
     3001 acatgttcgg gatcatcagg aggaggccct gggacgccgt gaacgtggtc gacagcgcac
     3061 cgccctgcag cgccccgtgc agcgcgccgg cggcaccacc ctcggactgc atctcgacca
     3121 cggtcggcac caggccgtac acgttggcgc ggccctgcgc cgaccactcg tccgccagct
     3181 cggccatcgt cgaggacggg gtgatcgggt agatgctgca cagctcgttg agccggaacg
     3241 cagccgaggc cgcggcctcg ttgccgtcga tgatcacgcg gctcatgcct tctcctcaac
     3301 catctcgatg gcgtgcaccg ggcactgttc gtagcaggtg gcgcagccgg tgcacttgtc
     3361 gtagtcgaag cggtagcgga agcccttgcc gagcttgatc accgcgtcct cggggcagga
     3421 accgaggcag ccgtcgcact cgaagcagtt gccgcaggac aggcaccgcg cggcctcgaa
     3481 atgcgcctgc tcgtcggtca ggccaccgac gatctcgtcg aagtcggtga tccggtcctc
     3541 gggctcgagc tcgggctggg agtgccggcg ggcgtcgccg aagtaccaga ggttcagccg
     3601 gtcgaagtcg gccaggtcgt gcttgggtcg ggccccggct tcgcgattgc ccagccaggc
     3661 gtcgatctgc tgggcggcac gcttgccgtg gccgacgccg atggtcaccg tgcgctcgga
     3721 cgggaccgcg tcgccgccgg cgaagatgcc ggggacgccg gtcatcagcg tccgcggatc
     3781 gacctggacg acgtcgtggt cgaagtgcat gcccggaatc cggcgcagga agttcgagtc
     3841 ggcctcctgg ccgagcgcga ggatgacggt gtcggcctgc aaccgctcga agcgtccggt
     3901 gccgcgggcg cgtccgctct cgtcgagttc catgatctcg acggtcaggt cgtcgtcctc
     3961 catcgaggag atggtgcgca gccagttcat cttgacgccc tcggcctcgg cgccttcctt
     4021 ctcctctgcg tgcgccggca tctgctcctc ggtgcgccga tagacgatga tcgactcctc
     4081 cgcgccgagc cggcgggcca cgcgggcggc gtccatcgcg gtgttgccgc cgccgtagac
     4141 agcgaccttg cgtccgatca gcggacgttc gcccgaggcg acatcgcgca ggaagctgac
     4201 cgcgtccacg atcttggacg cgtcccggtt cgggatgtcg atgcgcttgg acaggtgcgc
     4261 gccgatcgcg acgaagacgg cctcgaagcc gccctcggcc tggtcggcca tcagatcctt
     4321 gacggtgtag ttgggcacga agcggacgcc caggtcgacc agccgggaga tctcggcgtc
     4381 caggacgtcg cgcggcagac ggtattcagg gatgccgtag cgcatcattc cgccgggaac
     4441 gtcggaggag tcgcggatct ccacgtagtg gcccatcttg gccaactggt aggccgcgct
     4501 gaggccggac gggccggcgc cgatgatcag cacgcggcgg cccgaggaga agcggggctt
     4561 ctcgaacgtc cagcccttct cgatggccag gtcgccgagg tagcgctcga cggagtggat
     4621 gctgactgcg ccgtccagtt cttcgcggtt gcaggcggct tcgcaggggt gatagcagac
     4681 ccggccgtgg atggcgggga acgggttgtt cttcaccagt tcgcgccagg ccttttcctc
     4741 ttcgccggcc ttcaccaggc ggagccattc ctggatgttc tcgccggcag ggcacgcgtt
     4801 gttacacggt ggcagcaggt cgacatacac cggcatgcgg gtacggacgg gggctacgcg
     4861 gccgccctca acggcgaggt cgggaagtcc ggtcatatcg cgggccatgg cttggcaccc
     4921 ttctctgggt tcagatcagg gtcaatgtac ctcactagaa ctacggtgtt gacaagggca
     4981 tttatgcaat atatagagcg cgtaaatacc ccaggaggta tttgtcagaa tgttaagtca
     5041 ggctctcgaa ttctgctgga attgaaacag ccgccggccg gacgccgcga ccagacggat
     5101 gtaggcgtcc gtggcttcct tgttctggcg cagttgcgcc tcggcgcgct tggccatgcc
     5161 gccaccgatc gcggaacagg tcgctgatga tggagaacaa tgccagcagg tacgagatga
     5221 acacgatgat catcagcgcg taccagatgg tgtttccgag ggttggccag aattcgggaa
     5281 ttctttcctt tcagactccg attgccgaac gcatccggca atcgcgggtt catgagccca
     5341 acctgaacct atgctgaggt catgcgcgcg acgtgtgcgc agattgaatc ggagaacgaa
     5401 ttgagcactg ccgagacccc tgcgcgcgcg gcctggttcc gccggctgtt gcccgtcgag
     5461 ccgccgatcg gctcgggatc ggtggctgca ccgggagcgt tcggaagcga gccggcgctg
     5521 gctcggctgg cgcaactcga aggactggac ccgggtattc cggaggccac cagaacgcgt
     5581 ttgtacgagc ccgacggcgg ggcggagatt tccatcgtgc tgtggcacgg gttcaccaat
     5641 gcgccctcgc agttcggcga agttgcgcag aatttacgga cgctgggata ccgcgtcctg
     5701 gtgccgagaa tgccgtacca cggcgaggcc gatctgttga accgggagtt ggcaaagctc
     5761 accgtcgcgg acttggtaga gcaggtggat acgtgcgtgg acatcgcttc cgggctgggt
     5821 tcgaaggtct gggtgctcgg gctctcggcg ggcggcacga tggctgcgtg ggccgcggcc
     5881 acccgtccgg aggtgagccg agccgcgctg gtggcgccgc tgatcgcacc ggtcggcttc
     5941 ccgctgccgg tggtccgcgc gctggtgaag tggccggcga tcgtcccgaa gttctacatg
     6001 tggtgggatc cgcgggtgaa ggagaagctc accgaaggcc agagcccgta cgcctacccg
     6061 ggcttcccgg tgcctggact gtttccgttc ctgcacttgt cggaggcact tttcgaccat
     6121 tcggtcgcgg ccgggcacga gctggagcgg gttgccctgg tgaccaatcc gggcgacttc
     6181 gcgatccgga aggatgtcgc ccaaaggttc gtcgaggagg tgttcgcccc gcgggcccgc
     6241 gccacggttg aagcccacgt cgacggcgat ctgaagtgga tgcacgactt cgtcgaccct
     6301 tggggcggta acggatcgac cgagcaggtg gtggcgatca tcctggctgc gttcggtgtg
     6361 ggcgagccga ccgccggcgg cgtcctggtg cccccgctgg tcaccgagca gccctaagga
     6421 agcgctgatc aacgctccct gggctcctcc cccacgatcc cgtcaggctc agtcggcgtt
     6481 tctaggcgca ctcggcgcaa atgccgtaga actccagctc gtggtcgagc ttggtgaagc
     6541 tgtgctgggc agcgatgccg ccgaccagct tctcgatgga cggcaacgcg acttcgaccg
     6601 tccgtccgca cgaacgacag atcaggtggt gatggtgccc gggcgaacag gtgcggtagg
     6661 ccgtctgtcc gtccggggtg cggatcgcgt ccagttcgcc ggacgcagcc atcgactgca
     6721 gcgttcggta aaccgtggcc agtccgacac ccgaaccctg gtggcgaagc tggtcgtgaa
     6781 tctcctgcgc ggtcgcgaac gtggccagtg cttcggcggc ctgccgaatc tcggcgcgct
     6841 gtcgggtctg gcgcggggtg gccggctcag tgctcgtcat agtggtcacc gtgggcggcg
     6901 tgccgatggc cgtcgtggat gtagtcgacg tgtccggcgt gctggacggg ctggtgtccg
     6961 cactgctcgt tgtgcacgtg cgggtgcggt tcggttaggt cgtggctgac cggcgccagg
     7021 tcgtcggtca cctcgatgat cgggcggttc cgccgctgca gtgctcgcgc gagcggccag
     7081 ctcagcacga acaggacgat cgcgatcacc acgatcagcg agcctggggc ggtgttcagg
     7141 tagaacgagc cgaccgtccc ggtgaccgca gcgatcgcgc caagacccat ggacgcgaac
     7201 agtccgcgtc ggaagccgat catcacctgg ttcgccgtgg ccaccggcac caccatcagc
     7261 gcgctgacca gcaacagccc caccgtccgc atcgagacgg tgaccgtgac cgcggccagg
     7321 ctcaccacca ggatgttcag cagtcgcacg ggcaggccga tcgagcgcgc aaacgcctcg
     7381 tccgcagaga ccgtgaacag ttgcggcatc aggccgaccg cgaccaccag caccaccagg
     7441 ccgaggccgg ctatcgccca gaggtccccc gtcgccaccg tgagcaacga accgaacagg
     7501 taggcggaaa gggtgcccgc gccctgcccg gccaggccgg ccagcagcag cccgccggag
     7561 aggccgccgt agaacaggat cgccaggccg acgtcaccac tggcgcgtcc ggactcccgc
     7621 atcagttcga tgccgatcga gccgagcacg cagatcacga tcgccagcgg cagcggcgcg
     7681 gtgccggtga ggatcgccag gccgacgccg gccacggcga ggtggccgag cccgtccccg
     7741 agcaggctca gccggcgctg cactaggtag gtgccgatgg ccggggcagt gacgccggtg
     7801 atcagggcgg ccagcagcgc gcgctgcatg aaggcgaggg cgagccagtc catcacgcct
     7861 ccaatccggt gcgatgcgta cgaggacgcg gcagttcgtg cacctcgtgg ccgccgtggg
     7921 ccgcgcgatg gtcgtggtcg tcgtggtcat ggtcgctgtg gtcatggtcg tgctcgtggt
     7981 ggcggtagcc gggcggcatg acccgtccgt cgacgaggac gatcgaccgg tcgatcagcg
     8041 gacggaacgc gccgagttcg tgcagcacca tcacggtggt gcggccggcg gccttcagat
     8101 ttccggcaac cttggccagc agttgttggg tggcctggtc gacgccggcg agcggttcgt
     8161 cccagagcat caggtcggcc ccggtggcga tcgcccgcgc gatcaggacg cgctgctgct
     8221 gcccgccgga gagcctgacg aaggggttct tggcgcggtc tatgaggccc acctcctcca
     8281 gacccgcgtt gatcgccgtc cggtcggcat gggtttcggg gacgaacgga cgccggcgcc
     8341 ccaggcgtcc gctggcgacc acttcgcgca cggtggcctg ctgcacgttg atgctgctgc
     8401 gctgcggcac gtagccgatg cttcgccagt cctggaaggt gttcagcggg gtgccgaaca
     8461 gctcgatgct gccgcggtgc ggcaccagtc cgagcagcgc ccgcaacagc gtggtcttgc
     8521 ccgaaccgtt gttgcccatg atcgccacga gctctcccgg ctggacggtt aggctggcgc
     8581 cgcggatgat cgggacgccg tgcagcacca cgtccacgga gtcggcggcg atcaccggcg
     8641 gacgatcaga tgtcacgagc attggttggc cgttcgcagg gcggcgaggt tcgcgcgcat
     8701 gacctcaagg tagtcgttgc cgcgggagtc ggcgctgatg ccctcgagcg ggtcgagcac
     8761 gtcgacggcc aggccgaggt cgccggcgat cgaccgggac acgaccgggg agacgagggt
     8821 ctcgaagaag atggtggtca cgccttcggt gcgggcgatc tgctgcactt cggcgatccg
     8881 ggccgcgctc ggttccacgt ccggctcgag gccgcggatg cccacctggg tgagtccgta
     8941 gcggtgggcg aggtagccga aggcggcatg ggaggtgatg aacgtgttcc gttcgcactt
     9001 ggtcaggccg gtcttgaagt cctcatcgag ggcgatcaga tccttggtca gggcttcggt
     9061 gttcgctgtg aattctgcct tggcttcggg tttcgcctcg ctgagtgcgt cgcggacgtg
     9121 ttcggcgacc acgaccagat tggttgggtc gagccaggtg tgcgggtcgt cggcaccgtg
     9181 attgtggctg tcttcggcat ggtcggctgc ggagtggtcg tcggcgtccg cggtcaggaa
     9241 ggtgaggaag gtggcggtgt cgaccgtccg cttgggcgtg acctgcgtga ccgcattgtc
     9301 caccgcgggc tggaatccgg actggagcac caccaggtcg gcttcggcca gcccagccac
     9361 ctgtcgcggc gcgagttcga ggtcgtgcgg ctcggatccg ggcggggtga ggttcgtgac
     9421 cgtcacctgg ctaccgccaa tgcgctcggc gacgagctgg aacgggtaga acgcgaccgc
     9481 gacctgcagt ttcccgtccg caacgccggt gtcggacggg gctgacgcgc agccgctcag
     9541 cgccaacacg gacgcgaaca gcaacgcaat cgaggtcctt acgaacatga caatcattct
     9601 caataaggag ggagtggttg tcaactcgag cggcggactt cagccgagcc gcaccacgcg
     9661 atcgcagcgg gcgaccagat cggggtcgtg ggtgatcacg agcaccggag aatcggtcga
     9721 atcggcccag atgtcctcga tcagggcgtt ggcggtggca gcgtccaggt gttcggtggg
     9781 ctcgtccagc agcaggacct ggtagttccc gaccagcagc ctggagagcg cgacccgacg
     9841 ggcctcgccg cccgacaggc tctcgccggt ctccccgact ggccgcgccg ggtcgagctc
     9901 gagtccggcc ctgccgagcg cggaaaccac atcgccccta gtcgcgtccc ggttgccgat
     9961 cttgacgttc tcctcaaccg tggtggtgaa gatgtgtgcg tcctgtgcca ggtagccgat
    10021 ccgtccgcgc acctccaccg cgccggcgag cgccgggatc agcccgagga ccgtggccgc
    10081 taccgtggtc ttcccgattc cacttgggcc gaccagcgcc accttctcgc ctgctcggac
    10141 gctgaagctc agcccggcca ccaccggcgc cgagcctggc caaccggccg tgagcccgcg
    10201 caccgtcagg ctcggttcgg gcaccggcgc acccgggtcg gccagatcgc cggagccgac
    10261 cggttcggcg tccagcactt cgctcacccg gtcgagcgcg gcccgggcgc gcgtgctggt
    10321 ctgcgcgctc aggatcagcg tggccagcgc ctcatgcaga gccagggggg tgagtacggc
    10381 gaccgctagg aacaccggcg agagcgtgcc ggcgtgcacc tggttgccgg cgatgacgag
    10441 cgcaccgatc acagcggtgc cggcggccag cagctgggcg gcggcgccga caccacggat
    10501 ccacgccgac cgcgcggacg ccagccgtag ctgctcgtcg atcgctgcga agtcggccaa
    10561 ccggtcctcg gcggcgccgt aggccaccag gtcggacgcg gtccgggaaa gctcgcgcgc
    10621 cgcgtccgcc aactgcccgc gcaggggcag ggcgatcgcg tccgcacggg cggaagccgt
    10681 ccgcgacagc cacgggacga ccgccgcggc caccagcgag gtgagcagca gcacgccggc
    10741 cgagccggga gagaagaagg cgatgccggc cgaggtcacc agcagcacgg ccgccgacga
    10801 gacgaacggg atccacaccc gcaccaccag gtcctggatc gcctcgacat cggcgatcac
    10861 ccgggacagc aggtcgccgc gacgacggcc aagcagggtg gtgcgcgcga gtctgacgta
    10921 ggtctccagc cgcagcgccg actgcagccg cagcgccacg tcgtggccga ccagccgctc
    10981 gacgtagcgg aaagcgccgc gcgagatgcc gaagaaacgg acgccgaccg cggccgcacc
    11041 caggtacatc accggcgggt gctcggccgc gaacgacagc aaccacgccg agacgcccat
    11101 caacgccacc gaggccgcgg tcgccgcgaa ggccaggatg gtcgccaggc tcaaccgcca
    11161 gcgggcaccg gggacggctg cgaacagccg cttgagcagc ggcgtctcgg cagcctgctg
    11221 gtcgcccgcc tcaccaacaa ggtggacggt ttctnnnnnn nnnnnnnnnn nnnnnnnnnn
    11281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11341 nnnnnnnnnn nnnncaaggt ggacggtttc ttgtggtgtg agcgccgaag cgtccgctgt
    11401 gttcagggcg agcggggcgg acgtgacagc ggcgggctca atggcggggg cgatctgtac
    11461 cacccgatcg gcctcagcga gcacggccgg acggtggctc acgaccagcg cggccacacc
    11521 aagggcacgc aggctgtgca gcaggccggc ctcggtgtcg gcgtccaggc cggcggtggg
    11581 ctcgtccagc accagcagct cggcgcgtcc caacgtgacc cgcagcagcg ccctggccac
    11641 cgcgatccgg cggcgctcgc cggctgacac cccctcggcg tcgtcgccga ccacctggtc
    11701 gggcggcagt tcggtggcgc cggcgcggcg cagggcgtcc gtcactgcga ggtgggtcgc
    11761 atgcgggaag cccaaccgga cattgtcggc gacggtcccc gagaccagac cgggttcctg
    11821 gccgacgtag gcgatgtggt cgcgccagcc ggacgtacgc ccgatcggct cgccgccgat
    11881 ggtcacgctg ccgccagtgg gtgcgaggaa gcccatcacc gcgttcagca gggtggtctt
    11941 gccgccgccg ctcggcccgg cgagtgcgac gatctcgccc ggctgaatgt cgaaggacac
    12001 cggcgccagc gcgggtgtgt ccgttcctgg ataggtatgg gtcagctcac gcacgctgag
    12061 cagcgcggcg tcactcgtgg cggacggccc aacgtggcgt tcggagatcc cggcctgcgc
    12121 cgggatgacg gtggagccgg gctcaggacc gtcgatcagc gcgaaagcct gctcgacggc
    12181 ggcgaccccg tccgcggcat cgtggtagtg gaccccgacc tggcgtaccg gcaggtaggc
    12241 ctcgggcgcg aggaccagca cgaacaacgc cgtggccagg tcgagctggc cgtagaccac
    12301 gcggaacccg atggtcacgg cgatcacggc gacgctcagg gtggccagca gctccagcac
    12361 cagcgccgaa aggaaggaga cccgcagcgt ccgcatggtc tcgttgcgat gcgcggtctc
    12421 gctcttcaac aggccctgcg cctgggcctt cgcccggccg aagacctgca gcgtgggcag
    12481 gccggcgatc aggtcggcga agtggttggc gagccgcgtt tgcacggccc accggcgctt
    12541 cgtgatcgct tcggtggtcc agccgaccag cgccatgaac atcgggatca gcggcagggt
    12601 gacggccagg atgatcgcgg aggtcaggtc ggcgctcagg atcgccacac cgatgatcag
    12661 tggcacggtc acggcgagca gcaactgcgg cagatatttc gcgaagtagc cgtccagccc
    12721 gtccaggccc tgagtgatca gcgtcaccag gccgccgctg gccgtcgact ccagcggacg
    12781 ctcgatccgt gcaccgagaa tgtcccggcg caactgcgac ttgaccttcg cggacgcgcg
    12841 ctgcgcgagc cactcgttcg cccaggcgag cgcggccttc aaaccgaaca ccagcaccag
    12901 gccgccgacg gcccagctca gggtgctcag gttccgggtg gcgaagatgt cggccaccga
    12961 acgcgacaac agccacgcct gaccaagcgt caggatggct gttgcgctgc ccaccgcgac
    13021 cccggcgacc aggtaacccc tggtcgcgcg ggctctgcgg atgaggcgtg ggtcgatcgg
    13081 tcccgccatc aggcggcggc caccacctcg tctggcatgt tctcgacgcc gagccggcgg
    13141 cggaacaccc agtacgtcca cgaggtgtag gcaaggacga tcgggacgaa gatcaccgct
    13201 gccaccgtca tcacggtcag cgtgccttcg gtggacgcgg ccgtgaccac gttcaacggc
    13261 gaatcgacgg cacccgccgc gtccttgaag ccgaggcccg gccacatggt gaggaagatg
    13321 ccgatggcca cgaacaggat ggtcaccgcc gtcgagatga acgccaggcc attgcgatcc
    13381 tgcttattga accaccacgc ggccagcagg ccggcagccg aaagcaccag cagcccccag
    13441 ccgagcacgc cggtcggatg cacgaactgc tgggccagca ggaacacggc accaagcccg
    13501 atcgccacga agccggcctt ctcggcgaag gcgcgggcac ggtcgtgcac ctcgcccttc
    13561 gtcttcagcg cgaggtagag cgagccgtgg aacaggaaca gcgcgaccag ggtcacaccg
    13621 cccagcaggc cgaaccagct gaacagctgc cagaagtacg ggctcggcgt gaccacgtac
    13681 aggaaggagc tggtcgtgtc cggggccacc ggcaggccga tgatgaagtt cgcgaagccg
    13741 acgccgaaca ccagcgccac gatgaacgaa ccgatcgagg ccatccagtc gaagctgttg
    13801 cgccacttgg cttccgggtg cttggagcgg tactcgaacg cgatgccacg aatgatcagg
    13861 ccgaccagca ccaggaacag cggcaggtat aggccggaga acagcgtggc gtaccagccg
    13921 gggaaggcgg cgaaggtggc gccgccagcg gtcagcagcc agacctggtt gccgtcccag
    13981 accggaccga tggtgttgac gatgacccgc ttctccttgt cgttcctgcc gaggaacggg
    14041 atcagcatgg ccacgccgta gtcgaagccc tcaaggacca ggtagccgat ccagaggacg
    14101 gcgatcagga tgaaccagag cgtctgcagg acggtgggct cgatagccag agcaatcatg
    14161 tcagcgcctc tcagtacgca aaggtcaggg gagcgtcgtc gtcggtgagc acttcaggct
    14221 cgacgacatc gggcaacccg agcttcaggt agtgcatgaa caaggcgatc gcaaccacgg
    14281 cgagcgcgcc gtagatcacc gtgtagagca ccatggtggt agccacctcc caggcactga
    14341 cgcctggcga ctccgccgcc gcgaccggca ggacgccgtt cacgatgaag ggctggcggc
    14401 ccatttcggt gaagatccag ccgaaggaga taccgaagag cggtgccagc ggcgccgtcc
    14461 acatcaggct ggtccacagc ttggagtcct tcgggaggcc cttgcgcagc gtccacagta
    14521 cgaaggcgcc gatcgccatg gcgaggaaac ccaggcccat catgatccgg aaggtccagt
    14581 aggtcaccgg gatgttggga acccagtcgg acgggttcgc cagcaggccg gacgcggtca
    14641 gccgctcggc agcctcctgc tgcagccggt tgtccggcga gatcagtcgg ccggccgcgt
    14701 actccttggc cagcggattg atgccttcga ccttgccgtc gaaggtgccg gtcgcgagga
    14761 acgagaggac catggggatc ttgatcgaga agacctccct ggaaccgtca agtgtgccga
    14821 tggtgagcag cgagaacgcc gccggctgct cggtctccca aagggcttcg gcagcggcca
    14881 tcttcatggg ctgcacttcg gtcatgatct tgccctggat gtcgccggtg atgatcgtgg
    14941 ccacgcctgc gccaagcagc acccaggcgc cgaacttggt ggcccaggtg taggcgctga
    15001 tgtccttctg ctgcttctcg gacgggttcg cgaccttcgc gagcttggcc aggtgccagc
    15061 cggcgatgcc gaggacgagg ccaccggcgg tcatgtacgc cgtcgacaag atgtgcggca
    15121 aggtgaccag cgccaccggg ttgagcagca catcaagcag tgaggtcatc tcggcacggt
    15181 cggtgatggg gttgtaggtg gcgccgaccg ggttctgcat ccacgagttc gcggcgagga
    15241 tgaagatcgc cgagatcatg gtgccgacgg acgccagcca gatggtcgcc aggtgcacct
    15301 tcttgggcag gcgatcccag ccgaagatcc acagcccgat gaagactgat tcgaggaaga
    15361 acgccagcag ggcctccagc gccaacggcg cgccgaagat gtcaccgacg aatcgcgagt
    15421 actcgctcca gttcataccg aactggaatt cctggacgat gccggtaacg agacccatgg
    15481 caaagttgat caagaacaac tttccgtaga atttcgttaa ccgaagcatc ttttcatcgc
    15541 ccgtgcggac ccagatggtc tgcatgatcg ccaccagcat ggacagtgcg atggtgatcg
    15601 ggacgaagaa gaagtggtac accgttgtga tgccaaactg ccatctggca agggtctccg
    15661 cgctcatggc gggcaatcta ctactggctg tcgtagtccg caatgaatgt tctagcattg
    15721 cggcatggcg gtgacgggcg agcttgaaca ggcggtgatg gacatcctct ggtcgcgtcc
    15781 gtcgagccgg tccgtgcggg aagtccatga actcctttcc acagaccgcg atctcgccta
    15841 cacgacggtg atgaccgtgc ttgaccggct ggccaagaag ggcctgctgt tgaggaattt
    15901 ggaagggcgc gcctggctct acgcaccggc gcggtcgagg gtagaggaag ccaccgccga
    15961 ggtgctcgaa ctgcttcagc ggctaaccgc cgccgaacgc agccagttgc tggccaggtt
    16021 gagcgcggtg actcctgcct gagccgtggc gctatacccc cggtggtttg ttgaataccc
    16081 tgagcggtgc attcctccgc ttcggcgcag ggtggcggcg gcccttcgcg gctgtcagaa
    16141 cgggccaccg cccactcctg gaatcactcg gctgcgaacg cgtccagccg gtcggccaag
    16201 gcgagcgctt cagccttgag ggtgagcgcc tgatcgactc gcccggcggt gcggtagttg
    16261 gccgcctcgg ccaggcgaag ttcgacctgc tcgcgggcca gcgccatcat ctgctgcggg
    16321 gctagatcgg tgaccacccg ctcggcgtgg ccgaaccgca ccgcccccgc gacgaactca
    16381 ttcggcacac cgatggtggc cggggtggcc acgtagctgg tctcggcgtt ctcgatcgcg
    16441 ctgatcgcgt cccggacggc gtgcagtgcc accttgtcgc gccgcttcat cagcttcgcc
    16501 aagtcggcgc gcagataggt caacaaggtc atttcgtttc ctccttactc ctttgcagcg
    16561 aagctagagg ttcacgtaac gtgaaggtca agcagcttcc tggccaccgc gccgagctgc
    16621 ggatcctcgg tcacagcgag aattgcgtcg cgtccggctt cgccgagctc gttgagagcc
    16681 agtaacgccg ccatccgcac tgatgcgtcg aggtcgtgca gcaacgacga aaggagcgtg
    16741 gaggattgtg caccgagttc ggtggacaat cctccgccga gccggccgat ggcttcggca
    16801 gccaactggc gggtggctgg atcgagtgat tgcagggccg cagccatcgg ttccagcgcg
    16861 gccgggccca cctgaacgag cgcgtccagc agatcggctg acggcacgag tgacgaaccg
    16921 agcagcgcgg ccagtggaac cccggccgct gcggcctgac cggccggcag cgaggccagc
    16981 gctcggactg cactggtcac cacctccgga tcggacgcgg ccagcgcctc gctcagggtg
    17041 ggcagcacct cgggcccgcc cagcttgccg agcgcccgga cgaggagggc gcgcagtcgg
    17101 gcgtccgccg ccggccagcg gcccagcacg ccggcagccg cgtccggttg ctgcgtgagc
    17161 gcccagatca gtatctcttg aaccgcaggg tcggtctcgt tggcgagcgc gtccagcaac
    17221 tcggtcgtgc tgcgcggcgc agcagtgagc gctgcccgca gccggatgat cggatcggga
    17281 tgcgccagcc ggctggtgag cgcgatcact ccgaggacgt cctcccagct gcgctccggc
    17341 gtgtccacga gcgagcgcag tcgggacgcg agcgactgct cgtccgcgat cctctcctcg
    17401 agatgctcaa gatgtgaggc cagcgtggcg gacgcgtcca gatccgggtc gcccaatgcg
    17461 tccgcgatct cggcgaggct gagcccgagt gccttcaggt tctggatctg caggagccgc
    17521 accagatcgg cctcggtgta gagccggtag tcgccccacg accgctcgga cgcgctgagc
    17581 agcccgagat cgtcgtagtg ccgcagcgtc ctccgggtga gcccggtgcg ctccgcgacc
    17641 tccccgatcc gccaggtgcg agccatccgc ccagcctaga gccagcacca ggcggagtcc
    17701 gtccgccaat tcagcggtgc ccgtcgcacc cacgtctgct accgaaaccg gtaatttcgg
    17761 cagcaaacgc gaagctgggt agggatcggt cgaccggctg agggcatcgg gtcggcgtag
    17821 caactaggcg gcctcgtcct cgtccaccat cgcggcggcg aactgggact ggtagagccg
    17881 gtagtagtgg cccttcgcgg cgagcagttc ggtgtgcgag ccgtgctcga cgatggcacc
    17941 gtcctccatc accaggatca cgtccgcgtc ccggatcgtc gacagccggt gggcgatcac
    18001 gaacgaggtg cggtcggccc gcagcttgcc catcgcgcgc tgcaccagca gctcggtgcg
    18061 ggtgtccacc gaggaggtgg cctcgtccag gatcagcagc gacgggtccg aaaggaaggc
    18121 ccgcgcgatc gtgatcagct gcttctcgcc ggccgacaga ttggacgcct cgtcgttgat
    18181 caccgtgtcg tagccgtccg gcagcgaacg cacgaaccgg tcgacgtagg tggccttcgc
    18241 cgcggcgacg atctcctcct cggttgcccc tcgccggccg tacgcgatgt tgtcgcgaat
    18301 cgtgccgccg aacagccagg tgtcctgtag caccatgccg atctggtcgc gcaggtcgtg
    18361 ccgcggcacg ctggtgatgt ccaccccgtc cagggtgatc cggccggcgt tcaggtcgta
    18421 gaagcgcagg atcaggttca ccagggtcgt cttgcctgcc cccgtcggac ccacgatcgc
    18481 cacggtgtgt cccggctcgg cgaccaggtt caggtcggtg atcagcggac gctcaggggt
    18541 gtagctgaag tccacatgct cgaactccac ccgtccggtg accacctcgc tgagcttgcc
    18601 ggacgcgtcc gcgggctcct cttctgcgtc cagcacctcg aacacgcgct cggcggacgc
    18661 gacgcccgac tgcagcaggt tcgccatgga cgccagctgc gtgatcgggt gggtgaacat
    18721 ccgggagtac tggatgaacg cctgcaccgc gccgatgttc agcccgccgg tggccaccat
    18781 caggccgccg acgactgcga tcacgacgta gttcaggttc ccgatgaaga acatcaccgg
    18841 catgatgatc ccggagatga actgcgcacc gaacgcggcc gagtacagct cgtcgttctt
    18901 cttgttgaac tgggcctgca cctcgcggcg gcggccgaac accttcacca ggtcgtgacc
    18961 cgtgtaggcc tcctcgatct ggctgttcag ctcgccggtc gacttccagg tctgcgcgaa
    19021 gagcttctgc gagcgcttgc cgatcatcac ggtcagggcc atcgcgatcg ggatggtgat
    19081 cagcgagatc accgccagga tcggcgagac cacgaacatc atcacgacca cgccgatcag
    19141 cgtgaacagg ttcgacagga tctgcgccag cgtctgctgc agggtctgcg cgatgttgtc
    19201 cacgtcgttg gtcacccggc tgagcagttc gccgcgcggc tggccgtcga agtagcgcag
    19261 cggcagcctg gccagcttct ggctgacctc ctggcgcatc cggtacaccg agcgctgcac
    19321 gatgccgttg atcatccagc cggacgccca cgagagcagg aagaacatca cgtagagcgc
    19381 cagcgcgagc agcaggacgc gtccgaccgc gccgaagtcg atcgcggtct ggttgagcac
    19441 gtgcgcggag aagatcaggt cggtggcctg gccgaggatg accgggccca ccgcgttgcc
    19501 gatggtggcg aagatcgtca tcacgatcac cccgatgatc atcgcgatct ccggacgcag
    19561 gtggccgagc aggcgcttca tggacggtcc gaacgacacc gccttctcgc cgctgccgcc
    19621 gcccgggccg tggcccatcg ggccgccgcc gcgctgcgga ccgtacttcg gtcgctcgtt
    19681 ggccttcgtc ggcgcctccg ggggagcttc cacggcgact gccggctcgg gagcgctggt
    19741 ggacacggtg gtcggttccg gggttgtggt catgctgccg cctccgtgct gctgaactgg
    19801 ctctcgacga tctcggcgta ggtggggcag gcgtcgagca actcgtcgtg ggcgcccacc
    19861 ccgacgatcc ggccgtcgtc gaggacgatg atctgctccg cctcgcgaat ggtggagacc
    19921 cgctgggcga ccaccactac ggccgcccgt tcggtctccg gacgcagcgc tgcgcgcagg
    19981 gccgcgtccg tcgccacgtc gagagctgag aacgagtcgt cgaagacgaa gatctccgga
    20041 cgacgcacca gggcgcgcgc gatcgccagg cgttgccgct ggccaccgga gacgttggtg
    20101 ccgccctgcg cgatccggga gtccagaccg tccggcatct tctcgacgaa gtcccttgcc
    20161 tgggccactt cgagggcgtg ccagagctgc tcgtcggtcg cctccgggtc gccgaggcga
    20221 agattggacg caaccgtccc gctgaacagg tagggcttct gcgggaccag cccgattcgg
    20281 ctccagagca ggtcgggatc gagctcgcgc acgtccaccc catccactga aacgttgcct
    20341 tcggtggcat cgaagagacg cgggatcagg ttcaccatgg tcgacttacc cgagccggtc
    20401 gaaccgatga tcgcggtggt ctcgcctggc cggacgacaa agctgatgtc gctcagcacc
    20461 ggcgactcgg caccgggata ggcgaagctg acgtcgcgga actcgacctc gccgtgccgg
    20521 gcgagttcgg tcaccggatg ctcggcggga acgaccgagg aggacgtgtc cagcacctca
    20581 ttgatgcgtc cggccgagac cgctgcgcgc gggccgatca tcagcatcat cgtggccatc
    20641 atcacgctca tcaggatctg ggccagatag gcgaggtagg cggtcagctg gccgacctgg
    20701 aggtggcctt cggcgattcg gaaggcaccg aaccacacca ccgccacgct ggacaggttc
    20761 atcacgaaga agacggacgg gaacatggtc atcattcgcc ggccgaccgc ggtcgcggtg
    20821 gtggccaggt cgtggttggc ccggtcgaag cgctcggact cggttggttc ccggacaaag
    20881 gcgcggatca cccgcaggcc ggagatctgc tcgcgcagca cccggttgag ggtgtcgatg
    20941 cggatctgca tgctcttgaa caacggggtc atctggctga ccagcacgcc gatgatcgcg
    21001 gcgagcacgg ccaccgcgac gacgatcagc caactcaggc cgacatcctc gcgcagtgcc
    21061 atcacgatgc cgccgatcat catcaggggt gcgctgacga tcatggtcgc ggtcatcagc
    21121 acgaccatct gcacctgctg gacgtcattg gtattgcggg tgatcagcgt gggcgcgccg
    21181 aagtggttga cctccttggc cgagaacgac agggcccggt cgaagatggc gctgcgcacg
    21241 tcgcggccga agcccatcgc cgcacgtgca ccgaaccagg tggcgccgat ctggccggcg
    21301 atctgcacca ggctgacggc gagcatcacg ccgccgaggg tccagatgat ggcggtgttg
    21361 ccggtggcca caccctcgtc gatgatcgag gcattgaggc tgggcaggaa gagcgcggcc
    21421 atcgcctgca gcagctgcaa cagcagcacc gcgacgaatg gccagacata tggacgcagg
    21481 aagcgtcgca gtaggcggaa catctatccc cttctggcga tgccgtggag cacggtatcg
    21541 gctagttctt cgggttggcg caatggatgg tcgttcgaca gcgagaagct ggtggcgaac
    21601 gagagtgcgg acagtacctg agcggcgaac acagtcggca cggcgaactg gtcggcgtag
    21661 ggttcgagcg ctgcggcgac tgccgcggtg agctgggctc ggctgtcctt ggcgtgcatc
    21721 ggcggtggtg gtggccaggg tgggcggtgt ccgtccaatg atccctgagc tctacccgac
    21781 gatcccggag cctcagccaa cgatccctga gcccgacgaa gggtgtccgg gccggtcgcg
    21841 cttgttgaag ccggatggaa cagatgcgcg aacagcgacc gcgtgcgttc gatctcgctg
    21901 cgcaggattt cgacgacagc cgcaacccgg gcttcgaccg gttgggacgc aggcagtccg
    21961 gtcagcctgg ccatggcggc gtccggcgcg agtgcggcgt gaatggtggc gtggatcagt
    22021 tcctgcttgg tttcgaagac ccggaagatg gtgccttcgg cgaccccggc ggcggaggcg
    22081 atctcgcgcg tgctcacctc ggtgccctgt gcgagtagca gcggcaaggt ggcatcgatg
    22141 atggcctgac ggcgaacttc cggtgccatt ggcgtagctc tgctcacccg cacagcgtaa
    22201 tgagtgagcg ctcactcatc aagtcgaggg agccaaacta cccaggctcg tccgcaatgt
    22261 ggacgcaccg ggcccctgcc ggctgcctcc ggaaagcggt gcggtgtagg catttggcga
    22321 gctgaccgtc ccaattccca acttgatccg aatcaattgt tcgcgcattt gggttgcgcc
    22381 aaagcaatta tggatttcaa tagtcaagtg ttgtgtcttc gcttaggaga gcattcgcgg
    22441 gcattcggaa cactggcttt ccccgggggc ggcgggccat gcggccgtcg aggtcgactg
    22501 taaaacaaca gttcgctcac tttctcggcg gctgagcagc ggactggaat tcaaggctct
    22561 aggctgcaac ggtgcgagca caccacctac ccctgctcac gaccgtttcc gcaccgaccg
    22621 ttcactccga ctacgccgtg gtcgcggtca cgcgtccgga ctttgcggcg gacgcgtacg
    22681 tcggccagct ttggcgcgtc cccgtagacg gccgtccggc ccgccggatc acgcgcggct
    22741 tcgtggacac ttcgccgaag ctgtcgccgg acggaagcct gttggcgttc ctccggggct
    22801 ctgcggaagc cccaccccaa ctggcgctcg tgccggctga cggcggcgag ccgatggtga
    22861 tcaccgacgc cgaactcggc gtccgcgagt tcgctttcag cccggacggg aagcggctgg
    22921 ccttcatcgc ccaggtgccc gagcccgggc gctacggcac ggtaaaagat gtgggtcccg
    22981 gcagggagga cccgcggcac atcaccactg tgcagttcca gctgaacggg gtcggctaca
    23041 ccgtggatcg gcgggcgcac gtgttcgtgg tcgacgtgcc cgacccctac ggcgagcccc
    23101 cggtcaagcc gatcggtaac gcggccaagg cggacgtcga cttcgtcgcc gttccggtcg
    23161 ccactcagct ttcgtcgggt gatttcgacc accacgctcc gacctgggac ggggacaccg
    23221 tcatcgttgt ggccgcacgg cacgaaaccc gcgatggcga tcttcgcgaa gacctctatc
    23281 gtttcgagtc gccggacgcc gctccggtgc ggctgaccgc ggcggagagc acggtgggcg
    23341 tgatcggctc accggtcgtg gttggtgatg agatgttctt catcggcggc gaccttgggg
    23401 cgagcgggct cgacttcttc ggccacaacc cgggtgtgtt cgctatcccg cgctccggtg
    23461 gctcggcccg ccggttgacc gctgcggact cggtgcatgt cgacggtcag ttggtggccg
    23521 acggcgacgg cgtactggcg gtgaacctcg tccgcggagt gggtcgagcg atccgcgtgg
    23581 ccgcttccgg aatggttgag cttgacggat cgatggctga gcttgacgaa gcctcggtgc
    23641 tcgccatcgg tgccgcagcc ggccgccgaa tggccgtggt cgcgtccgcg gacagcccgg
    23701 gtgagctggt cgacctggcc cgtccggacg tgccgttgac cgatttcgct gccgcgctgc
    23761 gggcggcgtc cgcaccgctg cggccagtcg aactggaggc caagtccgcg gacggctatc
    23821 cggtgcacgg ctgggtgttc gccccgccca cgagcggccc gcacccggtg ctgctgatga
    23881 tccacggcgg cccgttcagc gcttacggac cgacgttctt cgacgaggcg caggtgctgg
    23941 cggaggccgg ctacgcggtg ttgatgtgca atccgcgtgg ttcagcgtcc tacggtgaag
    24001 cacacgcggc tgccatcaag cacgacttcg gcaacctgga cgcggccgac gtgctggcct
    24061 tcctcgacca cgccctggcg accgtgtccg gcctggacgc cgatcgcgtg ggcatcatgg
    24121 gcggctcgta cggcggctac ctgaccgcct ggctgatcgc gaatgagcac cgcttcgccg
    24181 ccgcgatcgt cgagcgggcc tatctcgacc cgaagacgtt cgtcggcgcg tccgacatcg
    24241 gctggtactt cccggccgcg atccacggcg aggatgtccg ttcgccgctg ctggtggtcg
    24301 accaggtgcg cacgcccacg ctggtgatcc actcggagaa cgatctgcgc tgcccgctgg
    24361 cggtggcgct gcgctactac accgaactca aacagcgcgg tgtggacgcg gagttgctgg
    24421 tgttccctgg tgagaaccac gaactctccc gaagcggaag cccgctccat cgcaaggcga
    24481 ggttcgagca catcctgcgc tggtgggatc ggcacctgcc ggtggtttct gcctagcgcc
    24541 gagactgggg ctgagcgcgg cggcgagcgt ccgggcggaa tcggtaacct atcccggtga
    24601 ctgagccgac ctccgcgaac ccgtccctgt ctgagcagga gcaggtgcgc catgataagc
    24661 gcgctcggct gctcgccgag ggggtcgcgc cgtacccgat cgaggtgcca cggacgcaca
    24721 ccgctgccca ggtgcacgcc cagtggagcc acctggtggc cggcgacgag accgcggatg
    24781 tggtgagcgt cagcggacgg gtcgtgttca tccgtaacac cggcaagctc tgcttcgcga
    24841 ccattcagga cgggttcacc caggccgatt ccggcgtccg actacaggtg atgctgtcgc
    24901 tggccgaggt cggtcaagaa cgcctggacg cgtggaaggc cgacgtcgac cttggcgact
    24961 tcgtctcggt gaccggccgg gtgatctcgt ccaagcgcgg cgaactgtcg gtactggcta
    25021 ccgcctggac gctggccagc aaggcgctgc gtccgctgcc gacgctgcac aaggagctcg
    25081 gcgaggagac ccgcgtccgg cagcgctacg ccgacctggt cgcccgtccg gaggcccggg
    25141 acatggtgcg cacccgcgct ctggtgactc gcagcatccg cgagactctg cacgccgacg
    25201 gctatctcga gatcgagacc ccggtgctgc agctggtgca cggcggtgcg gcagcgcgtc
    25261 ccttccagac gcacctgaac gctttcgaca tcgacatgac cctgcggatc gcccttgagc
    25321 tcttcctgaa gcgggcgatg gtcggcggcg ccgaccgcgt ctacgagatc ggtcggatct
    25381 tccgcaacga gggcatcgac tcctcgcatt cggcggagtt caccatgctg gaggcctacc
    25441 aggcgtgggg tgaccagaag tcgatcgccg acctcaccca gcagatgatc gtcaacgcag
    25501 cggacgccgc cctgggcacc cgggtgctcg acaccccggc cggtgagatc gacctggacg
    25561 gggagtgggc ctggaagccg gtgtatccga ccttgtcaga agcgcttggt gtggagatca
    25621 cgcccgagac ggacgcgtcc gtgctgcttc ggatcgccgc ggagcgtggc gtcgagcacg
    25681 atccggcctg gccggcgcag aagctggtga tggaactgtt cggcgaactg gtcgagccgt
    25741 ccctgttgca gcccactttc gtctgcgact acccgccgat cgcgcagccg ctggcgcgtc
    25801 cgcaccgcag cgacccgaac ctgatcgagg cctgggacct gatcatcggc ggcgtcgagc
    25861 gcgggacggg cttctccgag ctgatcgacc cggtgatcca gcgcgaacgg ctggtggcgc
    25921 agtcactcaa ggccgcggcc ggcgaccccg aagcgatgca actggacgag gacttcctcg
    25981 ccgccctgga gttcggcgcc ccgccgatgg gcggcatggg cctgggcatg gaccgcctga
    26041 tgatgctgtt caccggagcc ggcatccgcg agacgatcct gttcccgctg ctcaagcccc
    26101 acgcgtgagg gctacttcca gagggtggag agcgcgaagg ctgcggccat caggcccatg
    26161 ccgatcagga cgttccagcc agcgaggccg gtgagcaggg gcaccacgat gttcgtggcc
    26221 gaggtgacgt agtagaccac cagccagagc acgccgatca ggccgacggt gatgaaagtc
    26281 ggcgccaccc actggcggct gcccggcgcg acggcatggc gctgctcatc ggcccgcttc
    26341 tcggcgatct gggctcgatc ggtcagcttc ttcttctcag cagcggcctt gcgaaccttg
    26401 gactcaggca ccgggactcc tactagatcg gacaaccaag caaggataac cgagaaagct
    26461 gagagtcccg gttcgatggg agaatggacg catggcgcgg atcctggtgg tcgataacta
    26521 cgactcgttc gtctacaacc tcgttcagta cctggcccag atcggcgccg aggtcgaggt
    26581 ttggcgcaac gacgatccgc ggttcgcgtc cgctggttgg gcggacggct tcgacggcat
    26641 tctgttgtca ccgggcccag gcactcccga agaggccggc gtctgcatcg acgtggtccg
    26701 cgaacagggt ggcaagctgc cgatcttcgg cgtctgcttg ggtctgcagt cgatctcggt
    26761 ggccttcggt ggcgtcgtgg atcgcgcgcc tgagctgctg cacggcaaga cttcgctcgt
    26821 ccaccacgac ggacgcgggg tcttcagcgg cttgcccaat ccgttcacgg ccacccgcta
    26881 ccattcgctg gcgctcgacc cggagaccgt gcccgacgct ttggacgtca ccgccaccac
    26941 cgactcgggc gtgatcatgg ccgtccggca caagactcag ccgatcgagt gcgtccagtt
    27001 ccacccggaa tcggtgctca ccgagggcgg ctaccagatg cttgccaact ggctggtggc
    27061 gtgcggtgac gcggacgcgc ccagccgtgc ggtcgggttg accccgttga tggcgctgcg
    27121 tccctaggcg ccctccggct gcgcccttcg tgacgcgccc gcgctcccgt gcggccgccc
    27181 actggcccag tgcggtcatc ccggcgaagg ccgggatcta gattcggttg atcaacgctg
    27241 gctgagcctg acgaagcccg tagtcaccgc gcggatggac gacccttcga caagctcagg
    27301 ggtcgttgat cggcggtgcc tcggcgcaac cgtgggcccg gaatcgctca gctactccgt
    27361 gggttcgacg tcggtcggcg cgatgctcgc ggtttcggtg ggcgtggttg gcggcggagt
    27421 cgtcggggtg atcgccttgg cgagcgtcag cgtgatcttc tgcgtgtact tcaccgtagt
    27481 accagccatc ggatcctgag cgatcacagt ccccggaatc gcgttcgact cctgctcgac
    27541 gaacacgatc tccttcgtga aaccggtcga attcgcagtg ttgatcgctt cttcgcgcgt
    27601 catgttccgg aacggcggca tcgtggtcgt gccgctggct acccgtagga ccacctcggt
    27661 atcaagggcc accttggcgc cctctgcggg cgtcaccccg gtcacctcgc cctgcttggc
    27721 ggtgttgggt gcgtcccgat cctcctcgac gaccaccgaa gcgaagccgt tgtcggccag
    27781 cagtttcgtg gccacttcct ggttgagccc cttgaccgca gggatgaccg ccttcgccgg
    27841 acccttgttg acgtggatga tcacctcggt gccgatggcg acccgaccgt tcttcgggct
    27901 ctggtcgacc acccgtccga ccgtggtgtc gtcggggcct ggctcgttga ccaccttggg
    27961 ttgcagttgc ttgctgatca gctgctgttc ggcactggcc tgcaactggt ccaccaccca
    28021 cgggatctcg accgtctcga cccgattcgc gttcaccagg tagtagatcc ccatgccggc
    28081 ggccgcgacg atgagcgtca ccaggaacgc cacgagcccc cagcgccggc ggcgcggcgg
    28141 cggctcgtag gcgcgcgcgg ccgtccgggt gtcctcggtg gcgaggacgg tggtcgcgtc
    28201 gctcgcgttg gccggcactg gcggtaccgg cacgggcacc ctcgcggtca cctcctgacc
    28261 ggccagcagt ctgccgatgt cggcgcgcat ggccgccgcg gtctgatagc gagcggccgg
    28321 atccttggcc agcgccttca gcgtgatcgc gtccatcgcc ggcgtgatct ccgggtcgag
    28381 ctggctcggt gggaccggag cctcgcggac gtgctggtag gccacgctca ccggcgaatc
    28441 gccctggaac ggtggccgtc cgaccagcaa ctcgtagagc aggcagccgg cggagtagat
    28501 gtccgagcgc gaatccacgt gctcgccgcg ggcctgctcc ggcgacaggt actgcgcggt
    28561 gccgatcacc gctgcggtct gggtcatggt ggcggacgtg tcggcgaccg cgcgggcgat
    28621 gccgaagtcc atcaccttta cctggccgtt cggcgtgagc atgacgttgg ccggcttgat
    28681 gtcgcggtgg acgatgcccg ccttgtggct gtagctcagc gcctccagca cgccctgggt
    28741 gaactcgagc gccttgttcg gcatgatctg ccggccggtg cgcaggatct cgcggagggt
    28801 gtgcccctcg accagctcca tcacgatgaa cggcaccatc acgccggtgc ccgggtcgga
    28861 ctcctcgccg gtgtcgtaca cggccacgat gttcgggtgg tttagcccgg cggcagcctg
    28921 cgcctcgcgc cggaagcggg cctggaacgt ggggtcggac gcgaggtcga cccgtagctg
    28981 cttgatcgcc acgtcgcggc ccagccgcag gtcgcgggcg cggcgcactt cggccatgcc
    29041 gccgcggccg agcacagggc ccagctgata acgaccgccg agcagcctcg gctcatcgat
    29101 cgtcggttgc atcctgtcct cccgatatgg cgaagccaac tctcccaaac tttgctgagc
    29161 gttccaacct ggtcatcgca gcgcctcgat cacggccttg acgatcggcg cggccaccgc
    29221 gccgccggcg atgtcggtcg ccgggatctc cgcgtcctcg acgaaggcgc agaccgccac
    29281 attcgggtcg tccgcccagc cggtgaacca ggcatacggc cggcgctcgt tgtccgagtg
    29341 cgcggtgccg gtcttgccgc cgacggtcac tccgggcacc tgcgcccgtc cgcccgtccc
    29401 gcgctcgacc accgcaacgg tcatctcctg aagcgccttc gcgttctctg cgctcagcgc
    29461 caccgaatgc ttctgcgggg acgtctgcgt ggtcaccttc agctcccggt ccaggacgcg
    29521 gttgacgatg tagggctcca tcacgacgcc gtcattggcg atcgccgcgg ccaccatcgc
    29581 catctgcagc ggggacgcgg ccacttcgaa ctcgccgatc gcgctcatcg cggtctgggc
    29641 accgtccagt tcgtccggga acgtgctggc cgccgtccgc agatcggtga ggaacttgct
    29701 gccgaagccg aagctctcgg cctgcgcgcg tagcttcgcg tccccgagtt cggcgccgag
    29761 cctggcgaaa ccgggattgc acgagaccat cagtgcctgg gtgaggctga cctcgctgcc
    29821 gccgcagttg cccgagatca cccgggtcga accgggcagc ctgaacgagc tggcgtcgag
    29881 cttggattcg gggctcatcc cttcggcgag agcagcggcc gcggtgacca gcttgaaagt
    29941 cgagccgggc ggatagatct cggcggtcgc ccgattggcc atcggcttgt ccgggtcggc
    30001 gttcagccgc ttccacgccg ccgtggacgc gtccaggtcg tgcgtggcga ggtcgttggg
    30061 gtcgtagctc gggtagctga ccagggcttt caccgccccg gtcttgacgt cgatggcgac
    30121 gaccgcgccc ttgcgtccgt ccagcgcgtc ccaggctgcc ttctgcgctt tcgcgtccag
    30181 cgtggtctcg accgtgccgc cggggacggt tgacccggcg gccatattga tcagccgttg
    30241 caggaactgg gaatcgtcct ggccggagag ctcggcggaa cgggacgact ccaagtcgga
    30301 cgtccggtac aggtaggagt agaagccggt caccggggcg tagagcttgc cgttgctgta
    30361 ggaccgttgg aacttgtagc ggtcatcgac cggcttggac gcggccaccg gcgtattgcc
    30421 gaccatgatc gggccgcggt cctgcgcgaa gcgcgcgtcg gtgaccctgc ggttctccgg
    30481 acgctcgctc aggtagccct gctggccgac gtagaggtag ctgacgttca ccagcagcgc
    30541 ggcgaacatg atcatggccg ccaccgcgac cccacgaatt ggacggttca tgcggacgcc
    30601 cccgtggtcg ggatgttctg ggtggcctcg tcgtccgccg ttggaacggg cgatgcgccc
    30661 accggaatca gctgcgtggt ctcgtgctca agccactgtt caggctcagt taccacctgg
    30721 ctggccgggc tgcgcacctg atgggtgatc accagcagca ggctgatcat cacccagttg
    30781 gcgaccagcg aggagccgcc ctgcgacatg aatggcgtag tcaggccagt caacggcagc
    30841 agccgggtga cgccgccgat gatcgcgaac acctggatcg cgaacacgaa cgagagcccg
    30901 gcggccagca gcttgccgaa cggctccttg ccgaccagcg atgcgcggag tccgcgagcg
    30961 acgatgatgc cgtagacgag aacgatcgcg ctcaaacccg ccacgcccag ttcctccccg
    31021 agcgcagccg cgatgaagtc ggtcttggca agggggatca ggctcggtcg gcccagtccc
    31081 cagcccgacc cgaaagcacc gccccaggcg agcccgaact gggcctggat cacctggtag
    31141 ttccggtcga agtcggcgaa cgggttcagc catgccgaca cccgcacctg gacgtggctg
    31201 aacgccagga aagccccgta cgcgccggac gcgaacagca gcccgccgag gatcggccag
    31261 ctcggcttct cggtggccac gtagagcatc agcacgaaga gcccgaagaa cagcagcgag
    31321 gtgcccaggt ccttctggaa gaccaggacg gccaggctgg ccagccacat cagcaggatc
    31381 gggccgaggt cgcgcggccg cggcaactcg atgccaagca cgcggcgtcc ggccagcgcg
    31441 aggacgtctc gcttgtcgac gaggtaggcc gcgaaggcga tggtcaggac cagcttggcg
    31501 agttcggcgg gctggaagct gtaggagccg aggcggatcc agatctgcga gccgttcttc
    31561 tcctgtccga tcagcggcag cagcggcagc atcagcaggg ttatgccggc gaggaaaagt
    31621 aggtacgggt aacggttcag cagcctgtag tcgcgcagga agatgatcac cgccgcgccc
    31681 gcggccaccg cgatcaccag ccacatcagc tgcagttcgg ccgagtgcat cggtgggctg
    31741 gacgcgaggt cgagccggtg gatcatggcc aggcccaggc cgttgagcag catcaccatc
    31801 ggcaggatca gcgggtcggc gtacgggatc cgccagcgga tgatgatgtg cagcaccagc
    31861 ccgatggcgc cgaggatggt gaccgcccag ggccagttgg tgggcagttc cgcgtcccgg
    31921 ttgaggtggg tgagcaggta cccgccgatg ccgaggccga tcgcgaagac cagcatgaac
    31981 agctcgaccc cgcgacgctt gcgatagctg atgactgcgt ccatcagcac tccatgggcg
    32041 ccggtggggt ggcagattcg gtgggggtgg gtgcggcgga cggtgacgcg gagtcggtcg
    32101 gtttggccga tccggacggg tcggcggtac cgtccgcgga accgcttggg atccctgact
    32161 gcgtcgccga agtggacggc accgcggtcg cggtctggcg ggcgagccga gcggcgatgc
    32221 actgttcggc cttggcgcgg agggtgtcga gggtggcgtg cgcatccgtc agtgaggcgg
    32281 ccggcatggt ttcgagcacc cgctcgtggt agtacggcgg caggtcggcg acgcgcgtgt
    32341 cgtcggtgtg cacgacatgg gagagcggca ggttgaacac cggctcgggg atgccctgga
    32401 agatcgccac gtgctcggac gcggcgccga gatagaactg ctgctgggtg tagccgtacc
    32461 agagcgcgcc gccgccgccg atcgcaacca gcgggatcag caccgccagc accaccttca
    32521 cccaggtgga cgcgcgccgc ttggccactg gggagtagcg cagccgctcg gcggcaccgg
    32581 gatccggcgc ggccgggtgg tcgagcagca tcggcacttc agcggtggtt tccagggtgt
    32641 ccaggtcgag gttggccgcg gcacccagca cctgagtggc gccgtccggc tcgccctcca
    32701 cgacgtcggc gatgatgatg gtgatgttgt cgtggccgcc ggcttcgtgg gcgaggtcga
    32761 ccaggtcggc gatcacctgt tcgcgggggc cgtccagatg ggcggagatg gcagcgtcgg
    32821 tgaccaggcc gcagagtccg tccgagcaga tcagcaggcg gtcgccggcg cgggcgtcga
    32881 cgagttcgag gtcgggcagg tgttgcggct ggccgttgat caccttgagg atcagcgagc
    32941 ggtgcgggtg ttcgagggcc tcggcttcgg tgatgcgacc ttcgtcgacc agggtctgca
    33001 cccaggagtg gtcgcgggtg attcgggcca ggtcgccgtc ccggtagcgg taggcgcggg
    33061 agtcgccgat gttggccatg ccgaactgtg ccccgtcgaa catcagcccg cagacggtgc
    33121 tgcccatgcc gtccaacatc ggatcggcct cgatgagttc gctcatcgcg tccccggcac
    33181 tatcgatcgc ggctgcgatg acctcgagca tctcggggcc ggagtggtcg ccgtctgctg
    33241 ccttgagctc ccggatcgcg acggcggacg cgaggtcgcc ggcggcggcg ccgcccatgc
    33301 cgtccgccac gacgagcatg gtgggagaga cgtaggcgga gtcctggttg ttcttgcgca
    33361 ccaggccgat ctcggagtgg gcgaggtagc ggagcgcgag ggacattact tctccacgct
    33421 catcagggtg cggccgatgc gcagggtgtc gccgatgccg aacgcggtgg gcgtggtgac
    33481 gcgctggttg ttgacgaggc tgccgttggt ggagccgagg tcctcgacct ggtagccgct
    33541 ggccgtgcgg gtgatctggg cgtgtcgggt ggacacgtag tcgtcgtcga ggacgagctg
    33601 gcagtcgggg ctgcgtccga tcaggaggac gtcgccgagg gggagggtga ggccggcctg
    33661 gcggccgtgg gtgacgacca gggacttggg cagcttcttg gcctggcggc gggagagctt
    33721 cggtggagcg ggtgcgccgg gcggggctgg tggggcgagt tcggcggcgg agaccttgcg
    33781 gccgaagagg tcggtgcgga tgacgtttcc ggtgaagagg atgaacagcc agagcagggc
    33841 gatgaagccc agcttgagcg ctaggacgac caggtcagac atcgctcacc ggagacacga
    33901 tggccagccg ggtggtgccg atctcgatgc gactgccgag ggcgaggctg gcgtgccggg
    33961 tgcgctggcc gtccacggtg atgccgttgg tggacccgag gtcctcgatg tcgaggcgct
    34021 ggtcggtgcc gcggccgttg acgttgatgc gggcgtgcag gcgggatacg ccagggtcgt
    34081 tgatccggag gtcggcgtcg gtgccgcgtc cgatagtgac gccgggcggg gtgagcgggt
    34141 ggcggacgcc gttgacttcg acgacgaggt tggcggcgcg gatctcgctt ggggtggcgt
    34201 cctcggtcgc gtcgaccccg gcgacggcgg atgaggtgac ggtgaagcgt ccgactggga
    34261 gggtactgtc gaggtagtag tcgatggtga ccgggccgtt gaagatgtag ccggcgtcgg
    34321 cggcgtagct acgcagttgc gggatgatct cagaattaag ggtcttggag tagggcgtga
    34381 gccggtcgtg gtcgtgctgg gagaagccga tccggaagtc gttggggacg agcttgcggt
    34441 cgcggctgag cagcctggcc tcggcgtcga gttcgcgctg gagccgggcg gtgagttcca
    34501 ccggctgtac gtcgcccttg aaggcgcggg cgaacgcacc actgactgcg ctctcgagct
    34561 tcttctcgac gcggtcgaag acccccatcc gcttccttcc atcccgtgtg gcgctgacaa
    34621 gcctatccag cgccgaacgt caagcgtacg cgacgggcct gagcggtcct cagccttgga
    34681 gtgccaggtg aggttgggcg ggtgtttgga cggtcgtcgc ctggtcgagc gccaatcgcc
    34741 gggtcgagtt cgccgagacc cctagcacca cacccaaacg ccggctgagc ctgtcgaagc
    34801 ccacccgtgc cgcgatctgg acccgggagc ctccgctgcg gcgctggagc agctctggtc
    34861 tacgtcggtg tgccgattgg cgtcatggtt gtgggctgtc gccggctccg gtcctgccgc
    34921 cgaagcctcc cgtgtcgcga tctcggcaac gtttggtccg tcgtggccgt accacgctgc
    34981 tagggcgatg ccaccgctgc gagatcccgt catgactgct gccaacgccc cggttcgggc
    35041 gcgacgcgtt cgctgtgcta atctcttccg gctggctcct gagggagtcg ggcgcgagtg
    35101 gcggaatggc agacgcgcac ggttcaggtc cgtgtgtccg aaaggacgtg gaggttcaac
    35161 tcctctctcg cgcaccgtaa ggtgcctctg gctagggcat atgaattctt tcgcctagag
    35221 gttttgcacc tatcagcgtc gaagttgaag atgccggatc tcgctgacaa ggccaaacgt
    35281 acagttctgc tggcggctct caggaatggg cgcctctcgt ctacgatcga ggtgcccccg
    35341 gacttgcgct gacaaggggc tttgcggtcc gagtcgctgc cgagcgtcgt cgagactggc
    35401 cgcctgcccc tcattcgccc ccgggtcggg cgctccgaat cggcttccca ctagggctgg
    35461 aactctgcga ggcgcgactt cggccggctc gctgccccac gtgtgcccca cacccgcacg
    35521 gaactgcacg tccacagttg tgatgaggtt tcgcgtcgga gcttccgcag cggtcgacgc
    35581 cgaaggcagc ctttcctgtg tgcagctacg ccaaacccgc gcacgcccgc agcggccccg
    35641 gggtgcgcga ggtctgcgaa ggtcttccag gtgttgctgg aggcgggcgg tgctcaggtc
    35701 gacgcattga gcccgcggct gagagcgccc acgagttcct gtcgtcttcc gaactctgag
    35761 ggccacccag gagcgccgcg aacctgcagg cttcgggtca gtagtgcggc gtcgccaggc
    35821 cagcgtgcca ggagttccga caaccgttgc gggccgatca ggccagccac gtccacgccc
    35881 agcaggttcg cggcagcacg ctgaacgtcg gcctgttcgt cgatcaggat ggcgtttcct
    35941 tcggcggtgt catagagccg gtcctggacg tcgcggttct ctgcgtacca atggagggtc
    36001 agcgcgaggt cggccgcgtc cttggtctcg tggtatgcgg accggtcgag ccaggcgccc
    36061 agctttgccg cagtgaaccc ggcgacagtg gggatcctga cttccagggt cggtgccagt
    36121 gcgaggggaa gcgaggcggt gaggatctcc gcgaaggccc acacgctgaa tgggacttcc
    36181 cgggtgggtg gctcgacgat cccgtcggga tcctcgatct ccccgaacgg gagaagatcg
    36241 acgtcgatgc cggtaacccg gtaacggatg ccggtgtgac caaccgccgg gaaggtggtg
    36301 gcgagggttc ggtaggcatc ccaggaggcc agggccagtg cgagatcgag gtcgcgcgtc
    36361 gcagtggtct caaagtcgtg gccgagtgcg cggtgcagga tgttgcggca ccacgcacct
    36421 acgagcatga cgtctctcgg ggcaagtgac tgcgacgttg ccagcacctt ttcgaccaca
    36481 cttgctgccg gctccaggaa atcgtcagga actcgtgtca ggtccagcat gggactgcct
    36541 ccagtcttgg gcggcctggc gcacgcgggg gtcatcgctg gcggcgaggt cggcatagac
    36601 cagtggccac ggcgcgagct ggacgcccgg tcgggtgccg tctggggcgc tccagaaagc
    36661 tcggcgaacc tcgatgttcg tcggcccatc ggatcgccag cggttcacga ccggcaggag
    36721 cgagtcttcc ctgggtgtat agagagtgag tgttgccgga cgcaactccg gcactgccga
    36781 ctcgcccgag atatagagcg tgtcgggttc agctgctgga cgtacgtgct cgatggagcc
    36841 gctgtacgtg gcgcgggtga gtttggcccc gagcccggtt gggtaggcgg ctgcccacag
    36901 ctcaaggagg tcggactgac cggcgggtcg cgaacctgcc ctgtacccgg cctccgagag
    36961 caactgacgc gcgttgtgag cctgacccac cgagacgtcg gcggcgcggg ccaactcccg
    37021 cagaggtgcc tgccacaggt caggccacgc aagcagcgcg aaaatgacct gggcgcgggc
    37081 tgtgctgaaa aggttgcccg tcgctgccgc cggcgctacg cccttgggcc tggggcggcc
    37141 acggacgtcg atgagtacgt cgccgaacgt gatccaagcg ttcccggcag tatccaggta
    37201 ctgaacgccg gcccggcgca gtgcatcggc ggtccgcggt gatacgaagt tgctcaagac
    37261 gagcgccggg cctcgttcct tgccgatcca agcggcgtca gccaacgtga agctcgccct
    37321 cacgaacacg gtgtattgct gctcggatct gccccgcatc agatacgcgt cggcccagcc
    37381 ctcgtctggc aggcggctct gaatgccgaa ctcgctgagg agtcgggtgt acggcttagc
    37441 cgccgcttcg ttcattccct ccacctgttc aggatagctg aacattcgcc aaacctgaac
    37501 acgccttgct gtatggctgg gcggtacgag gcgtccggag gcgccgcggt catgaacgcc
    37561 agtgggccaa ggttggtcgg gcaggttcca aaccctgccc cacatctgcc ccacgaggcc
    37621 cgtcacacgg ttccagaact gtacataacc cctagtggaa gctatgttca cccgtgtcct
    37681 gtgggaggtt caactcctct ctcgcgcacc gtaaggtggc cctgactagg gcaaacacgg
    37741 aaacgcgtat cctctctcgg gaccatttcc gactaggccc caatctctct ggctagggct
    37801 ttcgctcggt gcggtgtggc gccttgtcgg gtcagttgag ccccttcgtg gccactcctg
    37861 ccccacggga atggtacggc gaaactgcct gtgactaggt gcggaaggcg gttgggcgcc
    37921 gcgtcgttga ggtccggcga acagcacagg tcgccaacca ggttcaggcg ccgcgaaccc
    37981 tcttgagaac tcatagaagc atgtctatca tatcgatcat catcaatcaa gcgatgagca
    38041 tctatagatc tggagtcctg gtgatcgact acctcaccac cctcgtcgcc actgtcgccg
    38101 gcgacgtgtg gaacacgctc gtccacaact ggccctacct gctcgccagt gtcgtggtcg
    38161 cctcgctcgt gcaggtctac gtcagcgccg atcacctcgc gacctggctg cggcgccgaa
    38221 cctgggtcgc catcctgggc gctgtcgccc tcggcgcgct caccccgttc tgctcctgcg
    38281 gcaccacagc catcgtcctc ggcgccatgg cctccagcgt cccgtgggct ccgatcgtgg
    38341 ccttccttgt ctcctcgccg ctgaccagcc cggaggagta cgtgttgtcg gtcggcctgt
    38401 tcgggcccgc cttcgcgacg accttcttcg ttgcggcgat cgccgccggc ctgatcggtg
    38461 ggggagcctc ctggtggctc gaaagccgag ggctgctcgc cgggcaagcc cgaatggccg
    38521 ccccagggtc cgcgtccagc tgcggcggcg cctgcgaaca gcaacccggc gacgg
//
LOCUS       DAOPRQ010000016        38125 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_831_length_38125_cov_12.721671, whole genome shotgun sequence.
ACCESSION   DAOPRQ010000016 DAOPRQ010000000
VERSION     DAOPRQ010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 38125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38125
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_831_length_38125_cov_12.721671"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(110..1120)
                     /locus_tag="PKJ61_03295"
     CDS             complement(110..1120)
                     /locus_tag="PKJ61_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012919252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOC12883.1"
                     /translation="MSDFDDSRLEDRGALAAADHLLRPLAEAGARVRREAASAELPLS
                     GLDLDDRPRAMIIFGPEARLLRAVLEPICPVPLVAWPRLGLPGWVGPLDLVVVMGGGD
                     KTSLAAAFEAVRRGCRLLVVSPPDSLLARQSASASTTLLPTTTGDPLAAAIVALGALH
                     RLGLGPQVNPESVASAMDQVAMDCSLTIDATQNPAKAVALELADAQPLVWGGSILAAR
                     ASRRIAEALRSASGRVVLSADAGALAPLLAEVSPRDPFADPFEEAAATQRPGLVLVDD
                     DLDDEHAAEERDRLRRYALAQDVLISTLHHNTGTEVERYVSVLQKGLFAAAYLQLGLG
                     RA"
     gene            complement(1122..1313)
                     /locus_tag="PKJ61_03300"
     CDS             complement(1122..1313)
                     /locus_tag="PKJ61_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016456478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HOC12884.1"
                     /translation="MSELPAEVLDLLVCPSCHTKLALDYEARELVCTGAECGLAYPIR
                     DGIPILLVDEARRPGNPSN"
     gene            complement(1306..2667)
                     /gene="manB"
                     /locus_tag="PKJ61_03305"
     CDS             complement(1306..2667)
                     /gene="manB"
                     /locus_tag="PKJ61_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846037.1"
                     /note="converts mannose-6-phosphate to
                     mannose-1-phosphate; the resulting product is then
                     converted to GDP-mannose by ManC which is then used in the
                     synthesis of mannose-containing glycoconjugates that are
                     important for mediating entry into host cells; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HOC12885.1"
                     /translation="MIQPQVFKANDIRGLVSGDQPEWDATGARAIGAAFAVAFGLAGA
                     EFVLGRDMRAGGRELSLAFVEGAREQGASVIDVGLASTDQLWFASGYTHLSGVQFTAS
                     HNPAGYNGIKFCLPDAAPITPALMARIVELSSQELAPAATRGDYRELDLLAAYAAHLH
                     SLVDLSGSRRLKVVVDAGNGMAGWTTPAVLGPLNVEVIGLFTELDGSFPNHPPNPLEP
                     ANLLDAQRAVRAHGADLALVFDGDADRCFIIDERGEVVSPSVVTALIAKAELAREPGA
                     TIVVNTITSRAVGEVVVAAGGELAISAVGHTKMKALMAQHRAIFGGEHSAHYYFRDFW
                     GADTGMLAGLHVLAMLGRSGVTMSELVAGLPQYAASGEINTSVADPAAISAAVASAFT
                     GRGELSFTDGLLVTGDGWWLSVRESNTEPLLRLNVEARDEATVASLRDDALAIIRKDT
                     ADE"
     gene            complement(2695..3039)
                     /locus_tag="PKJ61_03310"
     CDS             complement(2695..3039)
                     /locus_tag="PKJ61_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014598613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3499 domain-containing protein"
                     /protein_id="HOC12886.1"
                     /translation="MINRRCSRSACADAAVATLTFAYSDSMAVLGPLALRAEPGTYDL
                     CRAHSERLSVPRGWEVVRLPGDLDSPRASTDDLMALADAVRKVGLGESVPAPEPNVSR
                     RKGHLAVLADPQ"
     gene            3153..3587
                     /locus_tag="PKJ61_03315"
     CDS             3153..3587
                     /locus_tag="PKJ61_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HOC12887.1"
                     /translation="MKRRERHGRGMRGPLAAPNPLTGSPVPLRRPTRGIDFFTDCLRD
                     AVARTSDACPRALVGVDIGFEEVPSNLAAWRSELVPLAAAVPAHGGANGQVILFRRPL
                     EHRADSRADLRRLVQRALVEQLSALTGISPDELDPGADRDWD"
     gene            complement(3930..5300)
                     /locus_tag="PKJ61_03320"
     CDS             complement(3930..5300)
                     /locus_tag="PKJ61_03320"
                     /inference="COORDINATES: protein motif:HMM:NF040160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5719 family protein"
                     /protein_id="HOC12888.1"
                     /translation="MNRWVPPGAVVVMVGVATVVGTLAGAQAVPEQRVTAAPARVSVV
                     CPAFASATASVKVVAAATGAGLQVAKLSGGAADASAGQQQVLANATEPVRVSALLPDP
                     FGASSFATASSGPARGISAAGCVAPRTDHWFVGVDVRAEAQADLVVVNLDGTAASIDL
                     IAYSHDGRIAAPRGVEVAGNSSQAISLSSLPRGDDPVTIAASSSDGRFAAFLRQRSWS
                     GDTSLGGDWLAGAAVPATEPVIPGLAAGAGVRRLVITNPNERTTTVTVGFLGASGPGA
                     VPGAEQLEVPPETTRVLDVTTGLAGQAVALSLTSTQPVTAAVWQDTGAAVARHDPAFS
                     AATLPLPADSYWPLAAGGKASTTLVLANPAAEPAVATVTVAANTQAASPQEVSVPAGT
                     QVAVPVPAAATNTVRIQTAATSLRGALVTTRKLGKVQGLAVWPLADQGSGGTSASVVY
                     DPRLGR"
     gene            complement(5297..8239)
                     /locus_tag="PKJ61_03325"
     CDS             complement(5297..8239)
                     /locus_tag="PKJ61_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC12889.1"
                     /translation="MSAGSAESYEDPWAWLHAARTSAPPDYRQFHVVAGVLPVRGEPQ
                     HQLVREALADQVIDVEIRDDLPGDADGDWIWVVPDDTVPTEGSLTALLDRVRAEPDAA
                     VIGSLLIEPRRRGAGTIISDWAQTITKSGRVRPLTDPGEFYQGQLDAVRALGVPAGGM
                     LVRGDAWRFLGGLNEELPRDYWGLDFGWRANLAGYRVIAEPRAQLVDHSEYSDDAHLK
                     AAGLALVSGNTPAARRWLTMLRLIVGTLFTAVGLLLGKDPRRAGDELRGLARWLRDGS
                     LRRGIAERVASLPVTPEARRATQSLRPKRGAGLRRSVELVAARLAEWVRTFTGRSGGV
                     SLDEMTGDEFAEVGSGRYALPLAASGALVLLLGGLAAARGTFGAGSLAGAQLLPAPGN
                     WTQLVDAYVAPIAGAPGISGAPWTALAGLLSFVAMGNPEWLVTGTLLLAVPVSWLLAF
                     RLLRQLVADRRLAGVAALAYALAPAVLGGLNAGAFGLAAMAMLLPILGYSLWHWLVAE
                     RWSWRRAGAVAFWLLLACALVPAAWLGAVVAAIWTAIRARSARAWAQWALVLAAPGLL
                     LVGGWGGTLLRFPGRLLTGIEPALTPAAAPDAWLVLIGRATSGAPPLWVSIVFFAACW
                     LAAFIGAARRPGSAGLALFAGGVAAVVAMVLSRLVVELPLGVWARPQGLEWQLALVAG
                     LVLAASLGLDGMLGELRGHDLGLRHLATLGLALFGAVAIAIGAGWWVLAGQVGLTRST
                     VGNVPAFVRNAQDSATPGRTLYLEAVGDQIAWALLEDDFPRLGDSERGIAFSGSTAAR
                     DLAGSVAARLVGDSADDQILPDLVTLGVSNVVLTGGNGAQQLAIDNAPGLGQGTGNAT
                     QFVWPVPDSGIVLAVDGARRQLTGAGQQVAAGSAERVLRLAQPRDPRWQVRLGDTPLT
                     SVDGGEPGGQFALGAASGQLSIDLGAGSPAWRWVQLAGLALLGVLAAPSVRRREELGP
                     RRAAGGAQ"
     gene            complement(8316..8576)
                     /locus_tag="PKJ61_03330"
     CDS             complement(8316..8576)
                     /locus_tag="PKJ61_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017934826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HOC12890.1"
                     /translation="MQELFLVVEPEAEGLLGWQDRALCAQTDPEAFFPEKGGSTREAK
                     KVCLSCEVRAECLEYALANDERFGIWGGLSERERRRVKKRAI"
     gene            complement(8741..9379)
                     /locus_tag="PKJ61_03335"
     CDS             complement(8741..9379)
                     /locus_tag="PKJ61_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC12891.1"
                     /translation="MVENLKRIREPITWAVIAIVAANIVLGMVRLVLQLVADVPVPIA
                     FAEIGASLMNVSLVLAVVVLVCICFFIAPATRHALLVTKVAAWVLTIGVALTAVCWGM
                     GIAASANTLAVVFEFLGGFLDLLIKALAAGSLWVLVRGVNAGRIDTPSPALPELAPAK
                     PADVEVAKPEAATTWQRGEAVGAVWRTADEAAAGAPGAMRIPREPADPETEG"
     gene            complement(9417..12101)
                     /gene="pulA"
                     /locus_tag="PKJ61_03340"
     CDS             complement(9417..12101)
                     /gene="pulA"
                     /locus_tag="PKJ61_03340"
                     /inference="COORDINATES: protein motif:HMM:TIGR02103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051060 - pullulanase activity [Evidence
                     IEA];
                     GO_process: GO:0005983 - starch catabolic process
                     [Evidence IEA];
                     GO_process: GO:0051678 - pullulan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pullulanase-type alpha-1,6-glucosidase"
                     /protein_id="HOC12892.1"
                     /translation="MSRSAEVVPGLEEAPGYWLRADLIAWPPHRLPPGEDPAALDWRL
                     HWSTEERIDPTASEPVPWQTALLRFDQAGLPGALVQQFPHLHNYLVLRLPEAAVAEVP
                     EALRSQVALSVHLPSGRLVAAGQLQLPGVLDELYPVAAQTVLGPSWHDGAPTLRLWAP
                     TAREVALLLWSPDAPLHSPGDRRQMWRAEDGTWAITGESSWLGYRYRYEVAVNTWHHH
                     RLVVNQVTDPYSQALTVNSTHSVLVDPDDPELAPELWLTASAPVLEHPVDQISYELHL
                     RDFSISDSSVPKELRGSFLATTVDSAGMTHLRRLAEAGLNTVQLLPLFDNATVEEHPE
                     QQQRPLKDELKSLPPNSAEQQRQVAAFGGRSGFNWGYDPWHYFAPEGSYSSTLASADG
                     AARVIEFRMMVGAFHAAGLRVVLDQVFNHTASWGQDKSSVLDRIVPGYYHRLSATGEV
                     ENSTCCANVATEHRMAERLMVDACVHWAKRYRVDGFRFDLMGHHTRANMLAVRAALDA
                     LTVEADGVDGSAVTMYGEGWNFGEVADNARFVQASQGQLGGTGIATFSDRLRDAVRGG
                     SVMDADPRSQGFGTGLLTADNGSGTNGDLRAQGGRLAWYTDLVELGLAGNLRSFRLGS
                     NSRHTVLRGDQLDFGGRAAGYADQPDEVINYVDAHDNETLFDALTLKLHPATPMRSRV
                     RANAVCLSLATLGQSPVMWHAGTDMLRSKSFDRNSYNSGDWFNYLDFTMADNGFGAGL
                     PPEPDNGSTWPLIAPLLADARLKPTGDDIRRAHDQALDLLRLRASTRLFRLGSAERIL
                     AKVTFPTSDSWHSSPGVIVMAIDDRVDPVDERWSGVLVVFNATNWVVRQSVPLDLTGF
                     ELHPVQAHGSDEVVRASSVGPEWATVPPLTTAVFVRPR"
     gene            complement(12785..14584)
                     /locus_tag="PKJ61_03345"
     CDS             complement(12785..14584)
                     /locus_tag="PKJ61_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016669615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOC12893.1"
                     /translation="MGQINHEELRGPARINPADRAQLAESPVSMARVAKLFAGHHWAL
                     AAVTAIIVVASVVGLAQPFLLRAIIDEALPKGDLAHLLWLVGGMVFAAAVTGVLGVWQ
                     TWLATTMGQKVMHTLRVRVFNHLQAQSLAFFKRTRGGEIQSRLLQDVAGLQSVVTSTA
                     TSIAANLTTAVATAVAMVAISWRLSLLSLLVLPPAILLTRKVALVRRDLTAAKQRALS
                     DLHTQVEEALSINGAQLTKTLGTGPARERQFAQTSAELIDLEVRSQLAGRWRMATMGI
                     VFAAIPAMLYLVAGFPALSGGISIGTLIAFASLQAGIFRPIMGLLNVGAQWVSSMALL
                     SRIFGYLDLAIEVPDPVRPVHLDAGRVRGEVRFENVSYRFPDGTENALTDVDLTIEPG
                     QSVAVVGETGSGKSTLAALLVRLADPTSGRVAVDGVDVRDLDPVERSRIIGMVTQETY
                     LTHASIAENLREAAPDATEAELWTALAAAQVDEVVRVLPDGLDTVVGARGHRFSGGER
                     QRLAIARTLLVNPPILVLDEATSALDTDTERELQVALEALMAGRSTLTIAHRLSTVRD
                     ADEIVVLAGGRVVERGEHVELLAAGGRYAQLNR"
     gene            14632..15078
                     /locus_tag="PKJ61_03350"
     CDS             14632..15078
                     /locus_tag="PKJ61_03350"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOC12894.1"
                     /translation="MDSFPDALAEELLRVARRLRRDTQQRVAPLGLNPHQARALRVIG
                     QAAPLRPSAVAEELHIAARSATDSIAGLATSGLVDRRPDPADGRAHLLVLTPAGEAVL
                     AEVTTIRSEVAAEVFGRLTEAEQASLASLLQQLAADRAKPVANSSP"
     assembly_gap    15251..15350
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <15351..16355
                     /locus_tag="PKJ61_03355"
     CDS             <15351..16355
                     /locus_tag="PKJ61_03355"
                     /inference="COORDINATES: protein motif:HMM:NF017984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HOC12895.1"
                     /translation="CVQAQDAPLAQAMIALRADTTVAAVQQALASGEIVRTHVLRPTW
                     HYVAADDLQWLLELTSAKVESGMAARHRQLELSPARISAVLDLVASALAGREFRTRTE
                     LGQLLDQAGLLTPTDPQFGQQVGHLLLLAELRGLICSAPDARPEHRYALLDEVIGRVP
                     SLERADAVLALVGRFVAGHGPVALSDLQRWARVTLTEARAALARLGEEVAPVQLDGDE
                     LWYAPKAVRPASRPAQAWLLSTFDEAFLSYRTIPWPRSAAHPAGDDPYRFAEAGGGVV
                     LHDLADVGSWKRTRTRSGVRLTLAVDPELSAAAREAIDVAARRLIDTIETQGSVVPGS
                     "
     gene            16734..17546
                     /locus_tag="PKJ61_03360"
     CDS             16734..17546
                     /locus_tag="PKJ61_03360"
                     /inference="COORDINATES: protein motif:HMM:NF016143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HOC12896.1"
                     /translation="MAVGLVAALALHFTSGGQTPRLITPPSPSASSKPSPTASGTANP
                     KPTPTMPSDPIDILKKNPLYSLKVNAKCAAQSAPTTTAAFRKQVKALVDCQNKAWQKA
                     LAKTAIEFRKPSIKHYSSSIKSPCGALGTNFPASYCTADNTLYFSKASFTQGRYYRLA
                     VAEFVIHEYAHHVQTLARIFSHTDAMKEREASISRRIELQAHCMAQYGLTHSNLGFTA
                     ADRADLEYQFGYTSDAEGHGSAKAERYWGLRGLDATKVGACNTWKVKASLVK"
     gene            17915..18439
                     /locus_tag="PKJ61_03365"
     CDS             17915..18439
                     /locus_tag="PKJ61_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC12897.1"
                     /translation="MATTASWPAIAAAVVGTLLVTVCAALGAVQILVLNPLAAVPGAS
                     LAQIQQELAAHDESLGTPFVLAEMGFGVLLAIALLFIAVRIRLPLVVIAGGYLWLLIL
                     ATPAYFWASFGAGMSLADTFGISGGDYSPWAWPIYLVSALATGGLVAILVISGVKAVR
                     NTRPAVTISGNSAN"
     gene            complement(18436..19323)
                     /locus_tag="PKJ61_03370"
     CDS             complement(18436..19323)
                     /locus_tag="PKJ61_03370"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOC12898.1"
                     /translation="MQPDDLRAFMAEHPLGVVSTHNAERGPEAALVSFALADDGAILF
                     DTFADSRKMANLQADPRVAIVLGCTGSVSMQIEGVAKLPSAEEGFEWAAEYEHHFPGS
                     NVFKSGMTVVRVRPHWIRVYDASGPSPVVRELTPGVEPASGKDRVCARDEPMPDDVAR
                     LLASVPEWFSMPDANAEYVDAARSMETWTVRDAAGAVIGVTLVRRHFDHAAEIHLTVV
                     DRAHHGRGVGTAMLQAIEADARATGVRLIEVKTLGASHPDAGYARTRHFYERLGFLPL
                     EETELWGADNPCLILVKPL"
     gene            complement(19594..>19850)
                     /locus_tag="PKJ61_03375"
     CDS             complement(19594..>19850)
                     /locus_tag="PKJ61_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HOC12899.1"
                     /translation="QSYLTAGPKESRAWTIRKGATAPEAAGVIHTDFQKGFIKAEVIS
                     FEDLVAAGSVVAARAAGKARLEGKDYVMADGDVVEFRFNS"
     assembly_gap    19851..19950
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<19951..20460)
                     /locus_tag="PKJ61_03380"
     CDS             complement(<19951..20460)
                     /locus_tag="PKJ61_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015011412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HOC12900.1"
                     /translation="MALTIGIVGLPNAGKSTLFNALTRNNVLAANYPFATIEPNVGVV
                     GVPDERLPVLAKLFSSEKTIPATVSFVDIAGIVKGASKGEGMGNAFLANIREADAICQ
                     VTRVFRDPDVTHVDGAVNPGNDIETIRTELILADLQTIEKALPRVEKEARIRKERQPT
                     LIAFQEAQAV"
     gene            20587..21522
                     /locus_tag="PKJ61_03385"
     CDS             20587..21522
                     /locus_tag="PKJ61_03385"
                     /inference="COORDINATES: protein motif:HMM:NF016370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HOC12901.1"
                     /translation="MIPRTRLPEPLLQLAAAQAGVLSREQVLAFGITDRVVDRLVTQG
                     TWRRVTRGVFAASAETWLQLAWAGVLLGGTSAIIGERSAGYLHGIERNPPDQVTIFVG
                     RGGRVERDDRWRFLRSDRLGVKEPPRTRLPETVVDVAGACSDDELTAMLAEVIASGRA
                     RPEDILRVVASRSRVANRKLLTEILGDVADGTDSPLERRYLHSVERAHGLPRAERRRR
                     PAQRFRTDAWYRAFGLIIELDGAAYHRGNARSTDMTRDNLHQLNGITTLRFTWTHVVG
                     RPCAVARQVAVMLARNGWSGELRTCPRCCAEDFAP"
     gene            complement(21547..22791)
                     /locus_tag="PKJ61_03390"
     CDS             complement(21547..22791)
                     /locus_tag="PKJ61_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015069785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HOC12902.1"
                     /translation="MFIDDLAAFVTASPSSYHAAAEVARRLRTAGFTEQLEADPWQAE
                     GARYVIRDGAIIAWRSAVTTGPVGFRIVGAHTDSPGFKLKPEPSAYAFGWSQAGMEVY
                     GGPLLNSWLDREFGLAGRVVSRDGAVHLVSTPAWLRIPQVAPHLDRSVNDSLRLDKQV
                     HLKPVFGTASVDLLSAVAGILDLHRDEIDGHDLFAAVTEPPAVIGVAGEFFASARLDN
                     LSSVHAGLAALLAADASDQVQVLACFDHEEIGSETRSGASGPFLESVLRRIAGSLGWT
                     DDDLARAFARSFVVSADAGHALHPNYPQLHDPDHRPVVNAGPLLKVNANQRYTTEGPS
                     TARWLRTCRAAGVPTQPFVSNNSVPCGSTIGPLTATRLGIESVDIGIPLLSMHSAREL
                     CGVNDPKWLADALGAHFTGADL"
     gene            22949..24478
                     /gene="rmuC"
                     /locus_tag="PKJ61_03395"
     CDS             22949..24478
                     /gene="rmuC"
                     /locus_tag="PKJ61_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002521967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HOC12903.1"
                     /translation="MEFLGWLVVGIALGAIVAWLVLRAFAGKSDAEAVAETERLRSEA
                     ERARGETAQARSDATRVALEVAVAQSDAADARTERAEAQAILASARASAAAAEAKLAA
                     AEAARDGAIERAEKIAADREQLVNQFKALSAETVQQQTRQLNETAEQRQRATDLMLAP
                     VKESLQRFNERLAEVEKERAVMATDLRNQVDAVRLTGDTLRRETAALVTALRKPQVRG
                     AWGELQLKRVVELAGMVEHCDFVQQQTTQTTSGATIRPDLKVMLTDGRFIYVDSKVPL
                     SAFLDAHDAAEESDRSRLLQQFAKNVQTHVDQLSKKDYFKAEGGSTPEYVVLFLASEA
                     LAAEAYNQLPSLLEYAAERGIMLATPSSLIATLKTAAFTWKQVALAESANEVFNLGRE
                     LYDRLGTLGKHFDKVGRSLTSAVGAYNEAVGSIEGRVFPTARKLRELKLTDKELPSVS
                     ASEAAVRPLTSNELVEDAAGVAPIIGRAKLEELTRPQPTLDELVALEGPRAKGVTAQT
                     A"
     gene            complement(24498..24869)
                     /locus_tag="PKJ61_03400"
     CDS             complement(24498..24869)
                     /locus_tag="PKJ61_03400"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOC12904.1"
                     /translation="MRRPSWYVQARTAIAGGDRAGLARVQRAAETAGYRAILGPIGAG
                     SGPGRPADDPLTAREREVLDLVARGWSNRQIADQLRISPKTVSVHVSAILTKLGAATR
                     AEAALHARPATGPARVWGRLS"
     gene            complement(25141..25374)
                     /locus_tag="PKJ61_03405"
     CDS             complement(25141..25374)
                     /locus_tag="PKJ61_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC12905.1"
                     /translation="MLDSYVTQRLYEQQLSQLNVENERMRRIRECNQLAGDSPADGPL
                     PIAQWWNSLRARIDVFLTDHHWTHHPTAAGTAG"
     gene            25586..26827
                     /gene="ilvA"
                     /locus_tag="PKJ61_03410"
     CDS             25586..26827
                     /gene="ilvA"
                     /locus_tag="PKJ61_03410"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016669489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HOC12906.1"
                     /translation="MTIDANAVDAAAQRLAGVVTRTPLQPNDRLSAATGASIWLKRED
                     LQPVRSYKIRGAYNVIAQLDASERAAGVICASAGNHAQGVAFACARLGIRGRIFVPAT
                     TPRQKRERIIALGEGRVELVVTGDTYDEAAAASLAEAARTGASVVPAFDHPDTIVGQG
                     TVAKEIVEQLGRAPDVLVAPVGGGGMISGCLAYLSERYPQTLVVGAEPAAAASMAAAV
                     AAGQPVQLDRIDTFVDGAAVRRAGDLTFAEVQKHGPQLVAVPEGRICSEMLSLYQTEG
                     IVTEPAGALAAAALGLYQPEPGQTVVVLVSGGNNDVSRYAEIVERSLVYEGRKHYFLV
                     NFPQEPGALRRFLDEVLGPDDDIALFEYVKRSNRETGPALVGIELGDPNSFADLLLKM
                     DASPITFEVVASDSPFYRFLV"
     gene            complement(27018..27947)
                     /locus_tag="PKJ61_03415"
     CDS             complement(27018..27947)
                     /locus_tag="PKJ61_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008361907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOC12907.1"
                     /translation="MKKLTTILASTIAAIALTACASGDPLAAAPSTSSAPAGSSAAQP
                     AIVVGSANFPENELLAELYAGALEAKSIQVTRKLNIASRETYVPALKSGEIDLIPEYT
                     GAFAKYLNPDADTSDEATALASLKAALPENLVALEPSSAQDKDSITVTKETADKYSLK
                     TIEDLVPVAGELVLGGPPEWKERAAGIPGLKKTYGLEFKEFKPLDVAGPLTVQALKNG
                     QVQAANLFTTDPAIKANNFVSLEDTKSFFGAQNVIPVLTKSKATPEVSAALNAVSAKL
                     DTDSLATLVTKVVVDKADAADVAAEWLKASGLA"
     gene            complement(27963..28691)
                     /locus_tag="PKJ61_03420"
     CDS             complement(27963..28691)
                     /locus_tag="PKJ61_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012924317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOC12908.1"
                     /translation="MNLLWEWLADPAHWQGADGIPVRTLEHLLYSAITLVLAAVVAVP
                     LGLWIGHTGKGRVLVVNLVNGMRSVPTLGLLFVAVLVAGPLLAGNIAFLVPAIFVLVI
                     LAIPPILAGAYAGVESVDPSARDAARGMGMSPRQLLFDVEIPCALPLLFSGLRSAALQ
                     VVATATIAASVSVGGLGRFLIDGQAYRDYGQMAGGALLVALLAVLVDLVFAGVQRLVV
                     SPGLSAPRATGRCRRSIRRLIPST"
     gene            complement(28688..29332)
                     /locus_tag="PKJ61_03425"
     CDS             complement(28688..29332)
                     /locus_tag="PKJ61_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008361905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOC12909.1"
                     /translation="MKWFLDHLDEVGSLLVTHAVLAVIPLVIGLMLAIPLGWLARRYR
                     GLRTPLVAGTGLLYTIPSLALFILMPLVLGAGILNPLNVVVALTIYTVALLVRTVADG
                     LAAVPDEVTQAATAMGIGRVRRFFTVDLPLAVPVIAAGLRVAAVSNVSIVSVAALLGI
                     EQLGSLFTDGFARNFLDPIIVGILACMVLAVALDLAIHWLTRVLTPWQRAGRAA"
     gene            complement(29329..30456)
                     /locus_tag="PKJ61_03430"
     CDS             complement(29329..30456)
                     /locus_tag="PKJ61_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008361903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOC12910.1"
                     /translation="MISFTDVTKVYPGGTTAVGGLTLTAPTGKITVLVGPSGCGKTTS
                     LRMINRMIEPTSGLITIDGRDTANLNANELRRGIGYVIQSGGLFPHRTIVDNIATVPL
                     LLGWGRAKARAEALELLERVGLAPELANRYPNQLSGGQQQRVGVARALAADPPVMLMD
                     EPFSAVDPVVRDQLQDEFLRLQAELGKTIVFVTHDIDEAIKLGDQVAVLRVGGTLAQL
                     DSPERLLADPADDFVADFLGRDRGYRGLAFQAAPDLPIRREPTVALGASVADARQAAG
                     GEDWLLVVDEAGAPAGWVELSGLAGPIDRDDLHRGGTVARAGGSLRAALDAALSAPSR
                     RGVLADADGRLVGTVRAQDVLAAIERDQTLPEPRPSDRDAT"
     gene            complement(30440..32050)
                     /locus_tag="PKJ61_03435"
     CDS             complement(30440..32050)
                     /locus_tag="PKJ61_03435"
                     /inference="COORDINATES: protein motif:HMM:NF012447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid ammonia-lyase"
                     /protein_id="HOC12911.1"
                     /translation="MNHQPGVVVIGSELTPEQVADVAIGHATVALSGNEVLERLARAR
                     AVVDDALATGVPTYGLNRGLGPLRDSEVPDELMADFQRFVIVSHAAAIGEPLSRTEGR
                     AALLARINTLARGASGASEALFRGLFDLLRLDVIPVIPDQGSVGAGDLSQMAAIGLVL
                     IGSGRAWLPGQDEIVPGAVALAAAGLAPVQLAAKDSLALVGSNALSVATAALTQRRSV
                     LLAEGADLVAALTVEALGANLSPFDARALTARPLPGQQASGSRIRTALAGGDLARGVR
                     ASSSVQDAVSLRTVPQVHGALLDELDELAGVLTVELNCAPDNPFLDIESGEFISNGNF
                     SITNLAIRFDALRIALAHVAKLAERRVAMLVRQLRQGLPLVDQVQAVTSATGYVTPVI
                     LAQTASALLAQVKHAASPISLTGSPVGDGVEDHSSMAYSSVRVTDGVLDVVEQLLAVE
                     ALLAATVISMHQLRHPAELGSPVERLRAAIIDVTGRTQVTAEVIEWVIEALRRERRGD
                     DLSEMRAPVFRTPVSNHDQEVLRHDQLH"
     assembly_gap    32203..32302
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(32330..33448)
                     /locus_tag="PKJ61_03440"
     CDS             complement(32330..33448)
                     /locus_tag="PKJ61_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF917 family protein"
                     /protein_id="HOC12912.1"
                     /translation="MRELTSADARHAVLGGGILACGGGGWKHHGELMGTLATSLERPV
                     LASIDELPEATWVATVSAIGAPAAKDWEIRPVDYVHALQELIRVAEVPIGAVMTAQNG
                     YSTTVNGWIQSAVLGVLVLDSAGDVRAHPTGKLGGLGLGERAGYESIQVVSGGNRELH
                     GHFQSVNRGNLNTVDDVLRDISVRVGGFIASARNPVELDWVKANGAHGSISLALDLGR
                     DLAAAQASGNADAVVEAVAQRLGAQVVDAGPLRIEKRTRTIGGWDRGTFRIGDHTVPY
                     LNEYMAVDNDGGRVATYPDTIVILDRGTGEGLAVKDAGRGGQDVVLLVAKAGVLPVSS
                     SSIDQVGLRECEQIMGIDFLSHLDPSLRPGTAAYAGDA"
     gene            complement(33445..35199)
                     /locus_tag="PKJ61_03445"
     CDS             complement(33445..35199)
                     /locus_tag="PKJ61_03445"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HOC12913.1"
                     /translation="MPIVAQRGGELRARNWRAEGLLRMFENVLEVAESPDELIVYASL
                     GKAARSWPDAHAIVNALLELDEAETLVLASGAPVGVLSTGTPAPLVLSAVNNTVGRWS
                     DADRFYARAAAGQTMWGGLTAAAWQYIGRQGVLQGTYELFGTIARTHFGAAPGSEADA
                     LSGRWVLTSGLGGMGSAQPISARMLGLSSLTAEVDPVKAERLMATGALDLVTESLDDA
                     LAALHQAKSAAETVAIALLANAADVFAEIAARGVVPDIVTDQTAAHDARYGYLPRGYD
                     LAGWRTLRETQPRQVERDARASIAAQVEAMLALATAGAVVFENGNNLRVQAIEHLGAA
                     ATDQVNRIPGFMEAYLRPLFARGIGPFRWVALSGSVADQLVLDDLAARSFPRRPEVAR
                     WIELAREYVPQQGLPARSCWLGHGERSAFAVAVNELVADGTLVAPVLFSRDHLDSAAM
                     THPRIGTEGMRDGSDGSTDFPLLDGMLLAATGADLVAIHSGGGGYSGWMQSAGVSIVA
                     DGRAETAVRLRRALDADTGLGVLRHASAGYTEAEASVAAGGETGEAPVNWVRPASPSS
                     RRTSGHWNDSSASVRENR"
     gene            complement(35591..36556)
                     /locus_tag="PKJ61_03450"
     CDS             complement(35591..36556)
                     /locus_tag="PKJ61_03450"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HOC12914.1"
                     /translation="MPDAKRVVVAAPRGYCAGVDRAVVTVEKALDVYGPPVYVRKQIV
                     HNRHVVEDLEARGAIFVEELAEVPVGATVVFSAHGVSPAVHAEAADRGLKTIDATCPL
                     VTKVHNEAKRFAGQGIQILLIGHAGHEEVEGTTGEAPEHITLVQHPDDVAGVELADPS
                     KVAWLSQTTLSVDETWETVGRLREKFPALMDPPSDDICYATQNRQSAVKQIAAHSDLV
                     IVVGSRNSSNSVRLVEVALEAGAKASYRVDNASEIDEAWLDGVRSVGVTSGASVPDAL
                     VQGVLDFLTERGFPPAVEERLTEENLTFALPPELRKDLRRKTIGD"
     gene            36781..38007
                     /gene="xseA"
                     /locus_tag="PKJ61_03455"
     CDS             36781..38007
                     /gene="xseA"
                     /locus_tag="PKJ61_03455"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOC12915.1"
                     /translation="MPMPSSPEQPQPLRAVVAATKQWVERLNPIWVEGQLIEIKRRAG
                     SVTQFLTMRDTLAEVSVTLSTSLAVLDAAGPLTEGTVVAAWVRPTVWSKSGSLTFECR
                     ELRASGEGRLLAAIEQRKRMLQAEGLFDPALKKRLPFLPRRIGLITGTQSAAERDVVE
                     NIGRRWPAAVLEVRHTLVQGAAAVEQLIDAVRALDRMPEVDVIIIARGGGSLEDLLPF
                     SDEALVRAVHACRTPVVSAIGHETDTPILDLVADLRASTPTDAAKRVVPDATAEATAI
                     AHAIGRVRQAVLNRAGTETRRLAELRSRPVLRDPSASLEGHRERLAHQETRLRRAIEA
                     RLREEQLGLGHTIERVRSLSPRATLNRGYAILADADGGTVDRVAATSVGAAITARLAD
                     GRLELTVTDVEPQEQP"
     gene            38004..>38125
                     /gene="xseB"
                     /locus_tag="PKJ61_03460"
     CDS             38004..>38125
                     /gene="xseB"
                     /locus_tag="PKJ61_03460"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014847623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HOC12916.1"
                     /translation="MTKLSYEEARDQLVEIVRQLETGSVPLSESMKLWERGEELA"
BASE COUNT         6191 a        13236 c        12818 g         5580 t
ORIGIN      
        1 cgtcatcccg gcgtaggccg ggatctcgga ctgactcgga gatcccggct tacaccggga
       61 tgacggttgg gactccgcct gaacgagaaa ggaaccgtcc ccgttccgtt caggcgcggc
      121 ccaggccgag ttgcaggtag gcggcggcga agaggccctt ctgcagcacg ctgacgtagc
      181 gctccacctc ggtgccggtg ttgtggtgca aggttgagat cagtacgtcc tgagcgagcg
      241 cgtagcggcg cagtcgatcg cgctcctcgg ccgcatgctc gtcgtccagg tcgtcgtcga
      301 ccagcacgag gcccggacgc tgggtggcgg cagcttcctc gaaggggtcg gcgaacgggt
      361 cccgcggcga aacctcggcc agtagtgggg cgagtgcgcc ggcgtccgcc gacagcacca
      421 cccgtccact ggctgagcgc agcgcctcgg cgatccggcg gctggcgcgg gcggccagga
      481 tcgaaccgcc ccagaccagg ggttgcgcgt ccgccagttc gagggcgacc gctttcgccg
      541 ggttctgggt ggcgtcgatg gtcagcgagc agtccatcgc cacctggtcc atggcgctcg
      601 ccaccgactc cgggttcacc tgcggaccga gaccgagccg gtgcagcgca cccagcgcga
      661 cgatcgcggc agccagcgga tcgccggtgg tggtcggcag caacgtggtc gaagcggacg
      721 cggactggcg cgcgagcagc gaatccggcg ggctcaccac cagcagtcgg cagccccgcc
      781 ggacggcttc gaacgcggcc gccagactgg tcttgtcgcc gccgcccatg accacgacca
      841 ggtccagcgg gccgacccaa ccgggcaacc cgagccgcgg ccaggccacc agtggcaccg
      901 ggcagatcgg ctccagcacc gcgcgcagca accgggcctc cgggccgaaa atgatcatcg
      961 cccgcggccg gtcgtccagg tccagcccgc tcagcggcag ttccgcggac gcggcctcac
     1021 gacgcacccg ggcaccggct tcagcgagcg ggcgcagcag gtggtcggcc gcggccaacg
     1081 cgccacgatc ctccaaccgg gaatcgtcga agtcggacat gtcagttgct cgggttccca
     1141 ggccggcggg cttcgtcgac caggaggatc gggatgccgt cccggatcgg ataggccagg
     1201 ccacattcgg cgccggtgca caccagttca cgcgcctcgt agtcgagcgc cagcttggtg
     1261 tggcacgacg ggcagaccaa gaggtcgagc acctctgccg gcagttcact catcagctgt
     1321 gtcctttcga atgatcgcga gggcatcgtc gcgcagggac gcaacggtcg cctcgtccct
     1381 ggcctcgaca ttgagccgca gcagcggttc ggtgttcgac tcgcgcacgc tcagccacca
     1441 gccgtccccg gtcaccagca gaccgtcggt gaagctcagc tccccccgtc cggtgaaagc
     1501 ggacgcgacc gccgcggaga tggccgccgg atcggccact gaggtgttga tctcaccgga
     1561 cgcggcgtac tgcggcagcc cggcgaccag ttccgacatg gtcaccccgc ttcggcccag
     1621 catcgccagc acgtgcagcc cggcgagcat gccggtgtcc gcgccccaga agtcgcggaa
     1681 gtaatagtgc gcggagtgct ccccgccgaa gatggcccgg tgctgggcca tcagcgcctt
     1741 catcttcgtg tgcccgaccg cactgatcgc cagttcgcca ccggccgcga ccaccacctc
     1801 gcccaccgcg cgggacgtga tggtgttcac cacgatggtg gcccccggct cgcgggccag
     1861 ctcagccttg gcgatcagcg ccgtcaccac ggacgggctg accacctcac cgcgctcgtc
     1921 gatgatgaag caacggtcgg cgtcaccgtc gaaaaccagc gccagatcgg caccgtgcgc
     1981 gcggacagcg cgctgcgcgt ccagcaggtt cgccggctcc agcgggttcg gcgggtggtt
     2041 cgggaaactg ccgtccagct cggtgaacag gccgatgact tcgacgttca gcggaccgag
     2101 cactgccggg gtggtccagc cggccatgcc attgccggcg tccacgacca ccttgagccg
     2161 tcggctgccg gacaggtcga ccagcgagtg caggtgggcg gcgtaggccg cgagcagatc
     2221 gagctcgcgg tagtcgccgc gggtcgctgc gggcgcgagc tcctggctgg agagttcgac
     2281 gatccgggcc atcagtgcgg gcgtgatcgg cgccgcgtcc ggcaggcaga atttgatgcc
     2341 gttgtagccg gccggattgt gcgaagcggt gaactgcacg cccgacagat gggtgtaccc
     2401 ggacgcgaac cacagctgat cggtgctggc caggccgaca tcgatcacac tggcgccttg
     2461 ttcgcgggca ccctcgacga aggcgagact cagctcgcgt ccgccggcac gcatgtcgcg
     2521 gccgagaacg aactcggcac cggccaatcc gaaggccacc gcaaaggccg ccccgatggc
     2581 gcgggcgccg gtcgcgtccc actccggttg gtcgccggaa acgaggccgc ggatgtcgtt
     2641 ggctttgaat acctgtggct ggatcacgcc tgcgagccta gcgctagcgc cggctcactg
     2701 cgggtcggcg aggaccgcca ggtgaccctt gcggcggctc acattcggct caggcgccgg
     2761 gacgctctcg cccaggccga cctttcggac ggcgtcggcc agagccatca ggtcatcggt
     2821 ggacgcgcgc gggctgtcca ggtcgccggg cagccggacg acctcccagc cgcgcggcac
     2881 gctgagccgt tccgaatgcg cccggcacag gtcgtaagtg cccggctcgg cgcgcagcgc
     2941 cagtggaccc agcaccgcca tcgaatcgct gtaagcgaag gtcagcgtcg ccaccgctgc
     3001 gtccgcgcaa gcggagcggg aacaacgccg attgatcacg gcgtcacgtt acctctgtgc
     3061 accggccgga ggcgggaggc gtccgcggtg tcgctgtgat ggccggcacg ctcggcagtc
     3121 cgtcgccccc aggatcgcct aggctggccg ccatgaagcg gcgggaacgg catggacgcg
     3181 ggatgcgggg gccactggcc gcgccgaacc cgttgaccgg ctcgccggtg ccgctgcgcc
     3241 gtcccacccg gggcatcgac ttcttcaccg actgccttcg cgatgcggtc gcccgcacat
     3301 cggacgcgtg cccgcgcgcg ctggtcggcg tcgacatcgg tttcgaggag gtgccgagca
     3361 acctcgccgc gtggcgctct gagctggtgc cgctcgcagc tgccgtccca gcacacggcg
     3421 gggcgaacgg ccaggtgatc ctgttccgcc gtccgctaga acaccgcgcc gactcgcggg
     3481 ccgacctccg tcggcttgtg cagcgcgcac tggtcgagca actgtccgca ctcaccggaa
     3541 tcagccccga cgagctcgat ccgggcgcgg accgcgactg ggactgattc cgcaactgct
     3601 ctccacggtc ctgaccaccc ggatggttct agcccgtctg gtgggccggt cgagctcacc
     3661 ttcgctcggt cgagcctgtc gagaccacac cgtgtcgcga tctcgtcaac agaagctcca
     3721 ccccggcact caaccgcccc cagccgaccg cacaactccg aaatcccgtc acggcaaccg
     3781 cccgctacgg ctgatcgagc ctgtccagag ccacgggacg acctggtctc taccgttcca
     3841 agggcggctg gcgcgtcaag actcccgcgc catcaccggt tgaggccgct gatggcgcgt
     3901 cgtctagccc gacaagacag caaccgaact cagcgaccca ggcgcgggtc gtagaccacc
     3961 gatgccgagg tgccgccgga tccctggtcg gcgagcggcc aaaccgccag cccctgcacc
     4021 ttgccgagct tccgggtggt caccagcgca ccccgcaacg aggtggccgc cgtctggatg
     4081 cggacggtgt tggtggccgc tgccggaacc ggcaccgcca cctgagttcc ggcagggacg
     4141 gacacctcct gcggcgaggc ggcctgcgtg ttcgcggcca cggtcacggt cgccacggcc
     4201 ggctcggcag ccggattggc cagcaccagc gtggtgctcg cctttccgcc cgctgccagc
     4261 ggccagtacg agtccgccgg caacgggagc gtggccgcgc tgaacgccgg gtcatgccga
     4321 gcaacggcag caccggtgtc ctgccagacc gcggcggtaa ccggctgggt cgaggtgagg
     4381 ctcagcgcaa ccgcctgtcc cgccaatcca gtggtgacgt cgagcacgcg ggtggtctcg
     4441 ggcggcacct ccaactgctc ggcaccgggc actgcccccg gtccggaagc accgaggaag
     4501 cccaccgtga cggtggtggt gcgttcgttc gggttggtga tcaccagccg ccgaacaccg
     4561 gcgcccgcgg ccaatccggg gatcaccggt tcggtcgcag ggacggccgc accggccagc
     4621 cagtcccctc ccagcgaggt gtcccccgac caggaccgct ggcggaggaa cgcggcgaag
     4681 cgtccgtccg aactgctggc cgcgatcgtg accggatcgt cgccgcgcgg cagcgagctg
     4741 agggagatcg cctgggacga gttcccggcc acctccacgc cgcgaggggc cgcgattctg
     4801 ccgtcatggc tgtaggcgat caggtcgatc gaggccgcgg tcccgtccag gttgaccacg
     4861 accaggtcgg cctgggcttc ggcgcggacg tcgacgccca cgaaccaatg gtcagtgcgc
     4921 ggcgccacgc aaccggcggc ggagatgccg cgggccgggc cggacgacgc ggtcgcgaaa
     4981 ctgctggcac cgaacgggtc gggcaacagt gccgataccc gcaccggttc ggtggcgttg
     5041 gcgagcacct gctgctgccc tgcgctggca tccgcagcgc cgcccgagag cttggcgacc
     5101 tgcaaaccgg cgccggtggc cgcggccacc accttcacgc tcgcagtggc ggacgcgaac
     5161 gcgggacaaa ccaccgagac ccgggccggc gcggcggtga cccgctgctc cgggactgcc
     5221 tgagcgcccg ccagcgtccc caccacagtg gccaccccga ccatcacgac gaccgctccc
     5281 ggcggcaccc agcggttcat tgcgcacccc ctgcggcccg ccgcggcccg agttcctcgc
     5341 gtcggcgcac cgacggcgcg gccagcacgc ccagcagcgc aagtccggcc agctgcaccc
     5401 aacgccacgc gggcgaccct gcgcccaagt cgatcgaaag ctgtcccgac gccgcaccca
     5461 gtgcgaactg gccgccgggc tcgccaccgt cgaccgaagt cagcggtgtg tcgccaagac
     5521 gaacctgcca gcgcggatcc ctgggttgcg cgaggcgaag cacccgctcg gccgacccag
     5581 cggccacctg ctgcccggca ccggtgagct ggcggcgggc gccgtcaacg gccagcacga
     5641 tgccggagtc gggcacgggc caaacgaact gggtggcgtt accggtgccc tggccgagcc
     5701 caggggcgtt gtcgatcgcc aactgctgcg cgccatttcc cccggtcagc accacattgc
     5761 tgaccccaag ggtgaccaga tcaggaagga tctgatcgtc ggcgctgtca cctaccagcc
     5821 gggccgcgac cgagccggcc aggtcgcgcg cagcagtgct gccactgaag gcgatgccgc
     5881 gctccgagtc gccaaggcgg gggaagtcgt cctccagcag cgcccaggcg atttggtcgc
     5941 cgaccgcttc caggtagagc gtgcgtccgg gggtcgccga gtcctgggca ttgcggacga
     6001 aggccggcac attgccgacc gtgctgcgcg tgagcccgac ctggccggcc agcacccacc
     6061 agccggcgcc gatcgcgatc gccaccgcgc cgaacagggc cagaccgagg gttgccaggt
     6121 ggcgcaggcc gagatcgtgg ccacgtaact cgcccaacat cccatccagg ccaaggctcg
     6181 cggcgagcac caggccggcg accagggcca gttgccattc cagcccctgc ggcctcgccc
     6241 agacaccgag cggcaattcg acgaccagtc gggagagcac catggccacc accgcggcca
     6301 cgccccccgc gaacagcgcc agaccggccg agcccggacg ccgggcagca ccgatgaagg
     6361 cggccagcca gcaggctgcg aagaacacga tcgacaccca cagcggcgga gcgcctgacg
     6421 ttgcccggcc gatcagcacc agccaggcgt ccggtgccgc ggctggggtg agcgccggtt
     6481 caatcccggt cagcaggcgt cccgggaacc gcagcagggt gccaccccag ccgccgacga
     6541 gcagcagtcc gggcgcggcc agaaccagcg cccactgcgc ccaagcccgg gcgctccgtg
     6601 cccgaatcgc ggtccagatc gcggccacca cggcgcccag ccaggcggca ggcaccagcg
     6661 cgcaagccag cagcagccag aacgcgaccg ctccagcgcg tcgccatgac cagcgctcgg
     6721 ccaccaacca gtgccagagg ctgtagccga gaatcggcag cagcatggcc atcgcagcaa
     6781 gtccgaaagc gcccgcgttg agtccgccaa gcaccgctgg cgcaagcgcg taggcgagcg
     6841 ctgcaacccc ggccaaccga cggtcggcca ccaactgacg cagcagccgg aaagccagca
     6901 gccaggacac gggcacggcc agcagcagtg tcccggtcac cagccattcg gggttgccca
     6961 tggccacgaa cgagagcagc cccgcgagag cggtccatgg tgccccggag ataccggggg
     7021 ccccggcgat cggcgccacg taggcatcga ccagttgcgt ccagttcccg ggcgctggca
     7081 acaactgcgc cccagccagt gacccggccc cgaaagtacc gcgggcagca gccaagccac
     7141 cgagcaggag caccagagct ccggacgcgg ccagcggcag cgcgtaccga cccgagccca
     7201 cttcggcgaa ctcgtcgccg gtcatctcgt cgagactgac gccgccgctg cgtccggtga
     7261 aggtgcgaac ccattcggcc aaccgggcag caaccagctc caccgaacgc cgcaacccgg
     7321 caccgcgctt gggccgtagc gactgggtgg cgcggcgcgc ctccggcgtg accggcagcg
     7381 acgccacccg ctcggcgata ccccgccgca ggctgccgtc gcgcagccag cgtgccaggc
     7441 cccgcaactc gtcgcctgcg cgccgagggt ccttgccgag cagcagcccg accgcggtga
     7501 acagcgtgcc gacgatcagc cgcagcatgg tgagccaccg tcgcgctgcc ggggtgttgc
     7561 cggagaccag cgccagcccg gcggccttga ggtgcgcgtc gtcggagtac tcggagtggt
     7621 cgacgagttg cgctcgcggc tcggcgatca cccggtagcc ggcgagattt gcccgccacc
     7681 cgaagtcgag cccccagtag tcgcgaggca attcctcgtt cagcccgccg aggaagcgcc
     7741 aggcgtcgcc gcgcacgagc atgccgcccg ccggcacccc gagcgcgcgg accgcgtcca
     7801 attggccctg gtagaactcg cccggatcag tgagggggcg gacgcgtccg ctcttcgtga
     7861 tcgtttgcgc ccagtcgctg atgatcgtgc cggctcccct acggcgaggc tcgatcagca
     7921 gcgagccgat caccgccgcg tccggttcgg cgcgcacccg gtccaggagt gcggtcaggc
     7981 tgccctcggt cgggacggtg tcgtctggaa ccacccagat ccagtcaccg tccgcgtccc
     8041 cgggcaggtc gtcgcgaatc tcaacatcga tcacctggtc ggccagagct tcgcggacga
     8101 gctgatgctg cggctcgccg cgtaccggga gcactccggc gacaacgtgg aattggcggt
     8161 agtccggcgg agctgaagtt cgtgcggcgt gcagccaggc ccagggatcc tcgtaggact
     8221 cggcactgcc cgcgctcaca ctccacctct ccgaccggtg atcggttcaa ccggtggttc
     8281 agcctacccc ggcggcgcct ccgccccagg ctggctcaga tggctcgctt cttcacgcgg
     8341 cggcgctcgc gctcactgag cccgccccag atgccgaacc gctcgtcgtt ggccagggcg
     8401 tactcgaggc actccgcacg gacctcacag gagaggcaca ccttcttggc ctcccgagtg
     8461 gaaccgccct tctcggggaa gaacgcctcc gggtccgtct gggcgcagag ggcgcggtcc
     8521 tgccagccca ggaggccctc agcctccggt tccacgacaa ggaacagttc ctgcatgcga
     8581 tatctcctcg gaccgtgccc gcgtcgatgt tcccgcgaac tacctgtcca gaattacacg
     8641 cctgtcattc acgataagtc aaccccgatg cattaccatt tgtcgcccgc tgcgttcggt
     8701 caaattctgg tacgggctta ccaggccctc ccggacgcga tcagccttca gtttccggat
     8761 cggccggctc gcgcggaatc cgcatggcgc cgggcgcacc ggcggcagcc tcgtccgccg
     8821 ttcgccagac cgcaccgact gcctcgccac gttgccaggt ggtggccgcc tctggtttcg
     8881 caacctcgac atcggccggc ttggccggcg ccagttcggg cagcgcaggg gacggggtgt
     8941 cgattcgtcc ggcgttcacc ccgcgcacca gcacccacaa cgagccggcg gcgagcgcct
     9001 tgatcagcag gtcgaggaag ccgccgagga actcgaacac caccgccagg gtgttggcgc
     9061 tggcagcgat gcccatgccc cagcagaccg cggttagcgc caccccgatg gtcagcaccc
     9121 aggcggcgac cttggtcacc agcagcgcgt gccgagtggc cggcgcgatg aagaagcaga
     9181 tgcagaccag caccaccacc gcgagtacca gcgagacgtt catcagagac gcaccgatct
     9241 ctgcgaacgc gatcggcact ggcacatcgg ccaccagttg cagcaccaat cgcaccatcc
     9301 cgagcacgat gttcgccgcg acgatggcga tcaccgccca ggtgatgggt tcgcggatgc
     9361 gcttgaggtt ctcgaccatg cttcctccag agttcggcga cagcctaaca acggggtcag
     9421 cgtgggcgga cgaacaccgc cgtggtcagg ggcggcaccg ttgcccattc cggtcccacg
     9481 gagctcgccc gcacgacctc atcgctgccg tgggcctgca ccgggtggag ttcgaagccg
     9541 gtcaggtcga gcgggacgct ctggcgcacc acccagttgg tcgcgttgaa caccaccagc
     9601 acacccgacc agcgctcgtc gaccgggtcg acgcggtcgt cgatcgccat cacgatcacg
     9661 ccgggcgagg aatgccagct gtcggacgtg gggaacgtca ccttcgccag aatccgctcc
     9721 gcgctgccga gccggaacag ccgggtggac gcccgcaatc gcagtaggtc gagagcttgg
     9781 tcgtgggcgc gccggatgtc gtcgcccgtc ggcttcagcc gggcatcggc cagcaatggc
     9841 gcgatcaacg gccaggtgga gccgttgtcg ggttcgggcg gcaggccggc gccgaagccg
     9901 ttgtcagcca tcgtgaagtc gaggtagttg aaccagtcgc cggagttgta ggagttccgg
     9961 tcgaagctct tgctgcgcag catgtcggtg ccggcatgcc acatcaccgg gctctggccg
    10021 agcgtggcca gcgaaaggca gaccgcgttc gcccgcaccc gtgagcgcat cggcgtggct
    10081 gggtgcagct tgagcgtgag ggcgtcgaac agggtctcgt tgtcgtgggc gtccacgtag
    10141 ttgatcactt cgtccggctg gtcggcgtaa ccggccgcac gtccgccgaa gtcgagctgg
    10201 tcgccgcgca gcacggtgtg ccgcgagttc gagccgagtc ggaaggagcg caggttgccg
    10261 gccaaaccca gctcgaccag gtcggtgtac caggccagcc gcccgccctg cgcgcggagg
    10321 tcaccgttgg tgccgctgcc gttgtcggcg gtgagcaggc cagtgccgaa gccctgggag
    10381 cggggatcgg cgtccatcac tgaaccgccg cggacggcgt cccgcagccg gtcggagaag
    10441 gtcgcgatgc ccgtcccgcc gagctggccc tggctggcct ggacgaagcg ggcattgtcg
    10501 gcgacctcac cgaagttcca gccctcgccg tacatggtga ccgcgctgcc gtcgacaccg
    10561 tcggcttcga cggttagggc atcgagcgcc gcccgcaccg ccagcatgtt ggccctggtg
    10621 tggtggccca tcaggtcgaa gcggaaaccg tccacccggt accgcttggc ccagtggaca
    10681 caggcgtcca ccatcagtcg ttcggccatg cggtgctcgg tggccacgtt cgcacagcag
    10741 gtggaattct ccacctcgcc ggtggccgag agccggtggt agtagccggg cacgatgcgg
    10801 tcgagcacgc tcgacttgtc ctgaccccag ctcgcggtgt ggttgaacac ctggtcgagc
    10861 accacccgca gcccggcggc gtggaacgcg cccaccatca tgcggaactc gatcacgcgc
    10921 gcggccccgt ccgcactggc cagggtagag gaataggaac cttcgggcgc gaagtagtgc
    10981 cacgggtcgt acccccagtt gaacccgctg cgcccgccga acgcggccac ctgccgctgc
    11041 tgctcggcgc tgttcggggg gagcgacttc aactcgtcct tgagcggacg ttgctgctgc
    11101 tcgggatgct cctccaccgt cgcgttgtcg aacagcggca gcagctggac ggtgttcagc
    11161 cccgcttcgg cgagccgccg caggtgcgtc atgccggcgc tgtcgacggt cgtggcaagg
    11221 aagctgccgc gaagctcctt cggcaccgag gagtccgaga tcgagaagtc gcgcaggtgc
    11281 agctcatagc tgatttggtc gaccgggtgt tcgaggacgg gcgcggatgc ggtgagccac
    11341 aactcggggg ccagttcggg atcgtccggg tcgaccagca ccgaatgggt ggagttgacc
    11401 gtgagcgcct gcgagtaggg gtcggtcacc tggttcacga ccagtcggtg gtggtgccag
    11461 gtgttcaccg ccacctcgta gcggtagcgg tagccgagcc aggacgattc gccggtgatg
    11521 gcccaggtgc cgtcttcggc acgccacatc tgtcggcgat cgccgggtga gtgcaggggc
    11581 gcatccggcg accagagcag gagggccacc tcgcgcgcgg tcggggccca caaccgcagc
    11641 gtgggtgcgc cgtcgtgcca ggacgggccg agcacggtct gggccgcgac cgggtagagc
    11701 tcgtcgagca caccggggag ctgaagttgg ccggcggcga ccagccgtcc cgaaggcagg
    11761 tggacgctca gcgcgacctg gcttcgcaac gcttcgggaa cctcggcgac cgcggcttcg
    11821 ggcagccgca ggacgaggta attgtgcaga tgcgggaact gctggacgag cgctccgggc
    11881 agcccggcct ggtcgaatcg gagcagggcg gtctgccacg gcacgggctc gctcgcggtc
    11941 ggatcgatgc gctcctcggt cgaccagtgc agccgccaat cgagggcggc cgggtcttcc
    12001 cccggcggca atcgatgggg cggccaggcg atcagatcag cacgcagcca gtagccggga
    12061 gcctcttcga gacccggcac cacctctgcc gatcgggaca tggttgcgat tctgccactc
    12121 aatctgattc gtggtggtat cgctcccaca ggtgtctcgc ctagccagcc tgatttcttc
    12181 gggagccgcc agccccaacc ttcccgagcc ggtggtcgcg cggcagccag tcagggtcgt
    12241 caccgaatcc cgagccagtc ccgcttcccg acaaagtgga cggtttcgcg gccggacgcc
    12301 gccaaaacgg gccaccttgt ggaatcgcct ccgcggacgc gagcggggac ggctgtagcg
    12361 ggaggtgatg gccaaaccgg acgcccccgc agtgccgcat cgcggatcaa cccacttcta
    12421 gctggctatg agcgcccgac ttgggcttgc cgcgcaacca acccaacttc tgctggctat
    12481 ggggggcgaa tcaggcctca taaccagcag aagttgggtt gggcccatac gactgccgaa
    12541 gtgacccctc ataaccggtc agaagtgggt tcatccggca gcaagccagc ggcggcccca
    12601 tcgcgtgggt gcggatggct gcgccatcga ggatcaaccg catcaacctc gaccagccga
    12661 ccgcagccgg ggccagcctc ccgccaaagt ggacggtttc tcgccgagac gccgccaaac
    12721 gggccacctt gtggaatggc agagacgagc gagcccccaa agtcgtaaac gccgacgact
    12781 ctgctcagcg gttcagctgc gcgtagcggc cgccggcggc cagcagttcg acgtgctcgc
    12841 cgcgttcgac cacccgtccc ccggccagga cgacgatctc atcggcatcg cggaccgtgc
    12901 tcaggcggtg cgcgatggtc agggtgctgc ggccggccat cagggcctcc agtgcgacct
    12961 gcagttcgcg ttcggtgtcg gtgtcgaggg cggacgtcgc ctcgtccaac accaggatcg
    13021 gcggattcac cagcaacgtc cgggcgatcg ccagccgctg ccgctcgccg ccgctgaagc
    13081 gatggccgcg tgcgccgacc acggtgtcca gcccgtcggg caacacccgc accacctcgt
    13141 ccacctgagc ggctgccagc gccgtccaga gttcggcttc agtcgcgtcc ggcgcagctt
    13201 cgcgcaggtt ctcggcgatg ctcgcgtggg tgagatacgt ctcctgagtg accatgccga
    13261 tgatccggct gcgttcgacc ggatccaggt cgcgcacatc cacaccgtcg acggcgaccc
    13321 gtccgctcgt cggatcggcg aggcgcacca gcagcgcggc cagcgtcgac ttgcctgaac
    13381 cggtctcccc caccacggcc accgactgcc ccggctcgat ggtcaggtcc acgtcggtga
    13441 gggcgttctc ggtaccgtcc gggaagcggt agctgacgtt ctcgaagcgc acctcaccgc
    13501 ggacgcgacc cgcgtccagg tgcaccgggc gcaccggatc cggcacctcg atcgccaggt
    13561 cgaggtagcc gaagatgcgg ctgagcaacg ccatcgaact gacccattgg gcaccaacgt
    13621 tcagcagtcc catgatcggc cggaagatgc cggcctgcag gctggcgaac gcgatcaggg
    13681 tgccgatcga gatgccgccg ctcagcgcag ggaagcccgc gaccaggtag agcatcgccg
    13741 ggatagcggc gaacacgatg cccatggtgg ccatccgcca gcggccggcg agctgggagc
    13801 gcacttcgag atcgatcagt tcggcggagg tctgcgcgaa ctgccgttcc cgtgcgggac
    13861 cggtgcccag ggtcttggtg agctgtgccc cgttgatgct gagggcctcc tcgacctggg
    13921 tgtgcaggtc ggagagcgcg cgctgcttgg ccgcggtcag atcgcggcgg acgagcgcca
    13981 ccttgcgcgt gagcaggatc gccggcggca gcaccaacag cgacagcagt gacagccgcc
    14041 aactgatcgc caccatcgcg accgcggtgg cgaccgcggt ggtcaggttg gccgcgatcg
    14101 aggtggccgt gctggttacc accgactgca ggccggcgac gtcctgcagc agccgggatt
    14161 ggatctcgcc gccgcgggtg cgcttgaaga aggccagcga ctgcgcctgc agatggttga
    14221 acacccggac gcgcagcgtg tgcatcacct tctgacccat agtggtggcc agccaggtct
    14281 gccagacccc gagcaccccc gtcacagccg cggcaaagac catgccgccg accagccaga
    14341 gcaggtgggc gaggtcgccc ttgggcagcg cttcgtcgat gatcgcgcgc agcaggaagg
    14401 gctgcgccag cccgaccacc gaagcgacga cgatgatggc ggtgactgca gccagcgccc
    14461 aatggtggcc ggcgaacagc ttcgcgacgc gggccatgct gaccggcgat tcggccaact
    14521 gcgcgcggtc tgccggattg atccgggccg gtccccttag ttcttcgtga ttgatctgtc
    14581 ccatgcaaac tccatatagt gaggtaacca cactaaataa ggctggtcgg catggattca
    14641 ttcccggacg cattggcgga ggagttgctg cgggttgccc gccggctgcg ccgagatacc
    14701 cagcagcggg tggcgccgct cgggctcaac ccgcatcagg cgcgcgcact tcgggtgatc
    14761 ggccaggccg cgccgctccg cccgtccgcc gtggccgagg aactgcatat cgccgcccgc
    14821 tcggccaccg actcgatcgc gggcctggcc acgtccgggc tggtcgaccg ccgacccgat
    14881 ccggcggacg ggcgcgccca cctgctcgtg ctgacgcccg ccggcgaagc ggtgttggcg
    14941 gaggtcacca cgatccgatc ggaggtggcc gccgaagtgt tcggacgtct gaccgaagcc
    15001 gagcaggcat ccctggcgtc tctgttgcag cagctggccg ccgaccgggc gaagcctgtc
    15061 gcgaactcgt ccccatagac tggccgggct ccatcgaacg ccatcgaaat cgatcggcac
    15121 gggagccctg gaggaggtgg atccgctgag ccggctgggc attgacgtgt ttctggctgt
    15181 cgcggtcggc atctggttcg tggctttcgg caccgccaag ctgccgtcgc cgccatcgac
    15241 cggtcgccgc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tgcgtccagg
    15361 cgcaggacgc cccgctggca caggcgatga tcgcactgcg ggctgacact acggtggcgg
    15421 ccgtgcagca ggcactggcc agcggtgaga tcgtgcgcac gcacgtgctg cggcccacct
    15481 ggcactacgt ggccgccgac gatctgcagt ggctgctcga actcacctcg gcgaaggtcg
    15541 agtcgggcat ggccgcgcgg catcggcagc tggagctgtc acccgcgcga atctcggcag
    15601 tgctggatct ggtcgcgtcc gcccttgccg gacgtgagtt ccggacccgc accgagcttg
    15661 gccaactgct cgaccaggcc gggctgctga cgccgactga cccgcagttc ggccagcagg
    15721 tcgggcatct gctgctcctg gccgaattgc gtgggctgat ctgctccgca cccgacgcac
    15781 ggccggagca ccgctacgcg ctcctcgatg aggtgatcgg acgcgtcccc tcgctggaac
    15841 gggcggacgc ggtgctggcg ctggtcggac ggttcgtggc cggccacggc ccggtcgccc
    15901 tttccgacct gcagcgctgg gctcgggtga ccctcaccga agccagggcc gcgcttgccc
    15961 ggttgggcga agaggtcgcg cccgtccagt tggatggcga cgaactctgg tacgcaccga
    16021 aggccgttcg acccgccagc agacccgccc aagcgtggct gctctcgacc ttcgacgagg
    16081 cgttcctcag ctaccgaacg atcccctggc cgcggtctgc cgctcaccct gccggtgacg
    16141 atccgtaccg cttcgccgaa gccggtggcg gagtggtgct ccacgacctg gcggatgtcg
    16201 ggtcgtggaa gcggacgcgg acgcgcagcg gagttcgact gaccctcgcg gtcgaccccg
    16261 aattgtccgc ggcggctcgc gaagcgatcg acgtggccgc acgacggctg atcgacacga
    16321 tcgagacgca gggcagtgtc gtacccggct cgtaagctgg tcgcgatccg aaccccggga
    16381 gcgccgagtt gaccagcgag ccgaatccgt ccgactgggg acgcgctacg cccccaggtc
    16441 cattgggcgc gcccggacac gatggatcgg ccagcgcctt ctcggccggg ccaccccctc
    16501 cgcccggcgc ctggccgccc ccggctgacc aggcgtgggc gccaccaacc gggccgaacc
    16561 tgtgggcacc gcctaccgag cagccatggc ggccaggcgt cgaccctcac gggcaaccgt
    16621 ctgagcggcc atggatccat cagccagagc atctggccgg gcagtacctg gcgggaccag
    16681 cgccgtcccg gcaccgcggc tgggtgaccg cgctgtcgac aggtgcggtt ctggtggcgg
    16741 tcggcttggt ggccgcactg gctctgcact tcaccagtgg cgggcagacg ccccggctga
    16801 tcaccccgcc gagtcccagt gcgagcagca agccgagccc aaccgcgtcc ggcactgcga
    16861 atccgaagcc aaccccgacc atgcccagcg atccgatcga catcctgaag aagaacccgc
    16921 tctattcgct gaaggtcaac gcgaagtgcg ctgcccagtc ggcgcccacg acgacggcag
    16981 ccttccgcaa gcaggtgaag gcactggtcg actgccagaa caaggcgtgg cagaaggcac
    17041 tggcgaagac cgcgatcgag ttccggaagc cctcgatcaa gcactacagc agctcgatca
    17101 agtcaccctg tggagcgctg ggcacgaact tcccggccag ctactgcacc gccgacaaca
    17161 cgctgtactt ctcgaaggcc tcgttcaccc aggggcgcta ctaccggctg gccgtggccg
    17221 agttcgtgat ccacgagtac gcccatcacg tgcagacgct ggcccggatc ttcagccaca
    17281 ccgatgcgat gaaggagcgc gaagccagca tcagccgacg gatcgagttg caggcccact
    17341 gcatggccca gtacgggctg acccactcca acctgggatt caccgccgcc gaccgagccg
    17401 acctggagta ccagttcggc tacaccagcg atgccgaggg ccacggcagc gcgaaggccg
    17461 agcgctactg gggcctgcgc ggactggacg caaccaaggt cggcgcctgc aacacctgga
    17521 aggtcaaagc cagcctggtg aaatgaccca cgcggctgtt cgggcccacc tctgcgctac
    17581 cggccggttc accgtcggct ggtcgagcct gtcgagacca agcttcacgc cccgcgacct
    17641 tcatcctgta tggcgaaatg tcctgcacta gtcgggtagc gatgccgagc ggatccagac
    17701 caatctccgc aaggattgag gccatctacg ccaggaggcg ccgccagcac agaacgggac
    17761 aaatctgccg ccagccgccg gaatcgcgtg gctatcgtca gtcgaagcca tccagcggca
    17821 gctttgtccc gtttcgccag cagccggctt ggctgcgaca cctaggctcc caccgaacct
    17881 ggaagggaac cggacggaga gcgcgagcac aacggtggct accactgcat cttggccagc
    17941 catcgccgca gcggtcgttg gcaccctgct cgtgaccgtc tgcgccgccc ttggcgcggt
    18001 tcagattctt gtcctcaacc ctctggccgc ggtgcccggc gcatcgctag cgcagatcca
    18061 gcaagagctg gctgcgcacg atgaatctct cggcaccccg ttcgtgctgg ccgagatggg
    18121 tttcggggtg ctgttggcga tcgccctgct gttcatcgca gtccgaatcc gactgccgtt
    18181 ggtcgtgatc gccggcggct acctttggct gctcatcctg gccacgcccg cctacttctg
    18241 ggcgagcttc ggtgcgggca tgagccttgc cgacaccttc ggcatcagcg gtggcgacta
    18301 ttcaccgtgg gcgtggccga tctatctggt cagcgcgctg gccaccggcg gcttggtcgc
    18361 aatcctcgtg atcagtggag tgaaagccgt ccgaaacacg cgaccagcag tgacgatctc
    18421 ggggaattcg gcgaactaga gcggtttgac caggatcagg caggggttgt ccgcgcccca
    18481 cagttcggtt tcttcgagcg gcaggaagcc gagccgctcg tagaaatgac gcgtccgggc
    18541 gtagccggcg tcgggatgag aagcccccag cgtctttacc tcgatcagcc ggacaccagt
    18601 tgcccgcgcg tccgcctcga tcgcctggag catcgccgtg cccacgccac gtccgtggtg
    18661 cgcacggtcg accacagtga ggtggatctc ggccgcatga tcgaagtgtc ggcgcacgag
    18721 cgtcacccca atgaccgccc cggcggcatc ccggaccgtc caggtctcca tgctgcgcgc
    18781 cgcgtccacg tattcagcgt tcgcgtccgg catgctgaac cactcgggga cgcttgccag
    18841 taagcgggcc acatcgtcgg gcatcggctc atcgcgggcg cacacccggt cctttcccga
    18901 cgctggttcg acgcccggcg tcagttcacg gacgaccggg ctcggaccgg aggcgtcata
    18961 gacccggatc cagtgcggac gcacccgtac caccgtcatg ccggatttga acacgttcga
    19021 ccctgggaag tggtgttcgt actcggccgc ccattcgaag ccctcttcag cggaaggcag
    19081 cttggccaca ccctcgatct gcatcgacac cgatccggta cagccaagca cgatcgcaac
    19141 ccgaggatcg gcctggaggt tcgccatctt tcgcgagtcc gcaaaggtgt cgaagaggat
    19201 cgcgccgtcg tcggctaggg cgaacgacac cagcgcagct tcgggaccgc gctcggcatt
    19261 gtgtgtggag accaccccga gcggatgttc agccatgaac gcgcggagat catctggttg
    19321 cacgattggc actccttgaa tcgatccggc cggccccaga accctagtag gtctcggcca
    19381 ggccgggcct cattcctcga tagtggccag gacgcgcccg cgcctactcc ggggaggggt
    19441 agcccgacca tcccgggaac gcgccgtcca cgtccccggt gagtgatagg cacgaaccga
    19501 gttctcccga cgcctgcggt aatgccggtt ggctgcggaa gcgctagccc ggccccaggg
    19561 agcctatcca cgagaacccg ggtgtccgcg agcctacgag ttgaagcgga actccaccac
    19621 gtcgccgtcc gccatgacgt agtccttgcc ttccagccgc gccttaccgg cggcccgggc
    19681 ggcaacgacc gagccggctg cgaccaggtc ttcgaagctg atcacctcgg ccttgatgaa
    19741 acccttctgg aagtcggtgt ggatcacgcc cgcagcctcg ggagcggtgg cgcccttgcg
    19801 aatcgtccag gcgcgcgact ccttggggcc ggcggtgagg tagctctgca nnnnnnnnnn
    19861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn caccgcctgc gcctcctgga aggcgataag
    19981 ggttggctgg cgctccttgc ggatccgggc ctccttctcc acccggggca gtgccttctc
    20041 aatggtctgc aggtcggcca ggatcagctc ggtgcggatc gtctcgatgt cattgcccgg
    20101 gttcaccgcg ccatcgacgt gggtcacatc cgggtcgcgg aacacccgcg tgacttggca
    20161 gatcgcgtcc gcctcgcgga tattggccag gaacgcgttg cccatcccct cgcccttgga
    20221 cgcgcccttc acgatgcccg cgatgtcgac gaagctcacc gtcgccggga tcgtcttctc
    20281 cgagctgaac agcttcgcca gcaccggcag ccgctcgtcc ggcaccccga ccacgcccac
    20341 gttcggctcg atggtcgcga acggatagtt ggccgcgagc acgttgttgc gagtgagcgc
    20401 gttgaacagg gtggacttgc ccgcgttggg gagtccgacg atgccgatgg tgagagccac
    20461 gtcggcctag cttagtggtc accagcgtgg aggcttttcc accgaagtcg gtggggaatc
    20521 ctccgcgtta tccacagggt gcggttctcc tgacgcgatt cggccgatgt tgccgacagt
    20581 gtgggtgtga ttccacgaac ccggctgccc gaacccctgc tccagttggc cgcggctcag
    20641 gcaggcgttc tctcccggga gcaggtgctg gcattcggca tcacggatcg cgtcgtcgat
    20701 cgactggtca cccaaggcac ctggcggcgg gtcacgcggg gtgtgttcgc ggcgagcgcg
    20761 gagacctggc ttcagctggc ctgggccggt gtcctgctcg gtgggaccag cgcgatcatc
    20821 ggcgagcggt cggcgggcta cctccacgga atcgaacgca accctcccga ccaggtcacc
    20881 atcttcgttg gccgaggtgg acgggtcgag cgcgacgacc gctggcggtt cctccgctcc
    20941 gatcgcctcg gggtcaagga gccgccacgg acccggctgc ccgagaccgt ggtggacgtc
    21001 gccggcgcct gcagcgacga cgagctcacc gcgatgctgg ccgaggtcat cgcatctggg
    21061 cgagcgcggc ccgaggacat cctccgagtt gttgcgtcgc gctcccgggt ggcgaatagg
    21121 aagttgttga ccgagatcct cggcgacgta gcggatggga ccgacagccc cctcgaacgc
    21181 cgatacctgc acagtgtcga acgtgcgcac ggtctaccgc gtgccgaaag acgccgtcga
    21241 ccagcgcagc gtttccgcac cgacgcctgg tatcgcgcgt tcgggttgat catcgaactc
    21301 gacggcgccg cctaccatcg cgggaacgcc cgatcgaccg acatgacccg ggacaacctg
    21361 caccaactga atggcatcac caccctgaga ttcacctgga cccatgtggt gggacgccca
    21421 tgcgcggttg cccgccaggt cgccgtgatg cttgcccgga acggctggtc gggcgagctg
    21481 cgaacctgtc cccgctgttg tgcggaggat ttcgcaccgt agtcggtgga caatcctcca
    21541 cgttgattac aggtcggcgc ccgtgaagtg cgcgcccaga gcgtctgcca gccatttcgg
    21601 gtcgttcaca ccgcacaact cgcgggccga atgcatgctg agcagcggaa ttccgatgtc
    21661 aacgctctcg atgcccagcc gggtcgcggt cagcggaccg atcgttgagc cgcacggcac
    21721 cgagttgtta gaaacgaacg gctgcgtggg gacaccggcc gcacgacacg tccgcagcca
    21781 gcgcgcggtc gacgggcctt cggtcgtgta gcgctggttc gcgttcacct tcagcagcgg
    21841 cccggcgttc accaccggac ggtggtcggg gtcgtgcaac tgcgggtagt tcgggtgcag
    21901 cgcgtgcccc gcgtccgctg agaccacgaa cgaccgcgcg aacgcccggg ccaggtcgtc
    21961 gtccgtccag cccagcgaac cagcgatccg gcgcagcacg ctctccagga acggtccgga
    22021 cgcgccgctg cgcgtctcgc tgccgatctc ctcatggtcg aagcaggcca gcacctggac
    22081 ctggtcggag gcgtccgcgg ccagcagcgc ggccaggcca gcatgcacgc tggacaggtt
    22141 gtccagccgg gcggacgcga agaactcccc cgcgaccccg atcaccgcag gtggctcggt
    22201 gaccgcggcg aacaggtcgt ggccgtcgat ctcgtcacga tgcaggtcga gaatcccggc
    22261 gacggcggag agtagatcga ccgaagccgt gccgaacacc ggcttgaggt gaacctgctt
    22321 gtcaaggcga agactgtcgt tcacgctccg atccaggtgc ggcgccacct gcgggatccg
    22381 cagccaagcg ggggtcgata cgagatggac ggcgccgtca cggctgacga cccgtccggc
    22441 aagcccgaac tcgcgatcaa gccaggagtt gagcagcggc ccgccgtaga cctccatgcc
    22501 cgcctgcgac cagccgaacg cataggccga cggttccggc ttgagcttga agccgggtga
    22561 atcggtgtgc gcgccgacga tccggaatcc gaccggcccg gtcgtgaccg ccgaacgcca
    22621 agcgatgatc gcgccgtcac gaatgacgta gcgggcaccc tccgcctgcc acggatcagc
    22681 ttcgagttgc tcggtgaagc cggcggtccg gagccggcgc gcgacctcgg cggccgcgtg
    22741 gtagctggac ggggatgcgg ttacgaaagc agccaggtcg tcgatgaaca tgcgcccaag
    22801 tgtgcacctt ccgcggatct gcgctgactg tggctgagcg caccacggac ggtgggccga
    22861 gccacagtga aggcttctgg acgacggttt gcaggccgct gcgtggcggt tggcagaact
    22921 gtcagtgcgg gcaggcaaga tcattgccat ggagttcctg ggttggttgg tggtcggcat
    22981 cgcgctcggc gcgatcgtcg cctggctggt gctgcgcgcg ttcgccggca agtcggacgc
    23041 ggaggcggtc gccgagaccg aacggctgcg atccgaagct gagcgggcac ggggcgagac
    23101 cgcgcaggcg cgctcggacg caactcgggt ggccctcgaa gtcgcggtcg cacagagcga
    23161 tgccgccgac gcccgcaccg agcgggccga ggcccaggcc attctggcga gcgcccgggc
    23221 gtcggctgct gcagccgaag ccaagctcgc ggctgctgag gccgcgcgcg acggcgcgat
    23281 cgagcgcgcg gagaagatcg ccgccgatcg cgagcagctg gtcaaccagt tcaaggcgct
    23341 ctccgccgag actgtccagc agcagacccg gcagctcaac gagaccgccg agcagcgcca
    23401 gcgggccacc gacctgatgc tcgcacccgt caaggagagc ctgcagcgct tcaacgaacg
    23461 cctggccgaa gtggagaagg aacgcgcggt gatggccacc gatctgcgca accaggtgga
    23521 cgcggtccgg ctgaccggcg acaccctccg ccgcgagacc gccgcattgg tgaccgcgct
    23581 acgcaagccg caggtgcgcg gcgcttgggg cgagctgcaa ctcaagcggg tggtagagct
    23641 ggcgggcatg gtcgagcact gcgacttcgt gcagcagcag accacccaaa ccacgtcggg
    23701 agcgaccatc cggcccgacc tgaaggtgat gctgaccgac ggccggttca tctacgtcga
    23761 ctcgaaggtg ccgctgagcg cgttcctgga cgcgcacgac gctgcggagg agtccgatcg
    23821 gagccggctg ctgcagcagt tcgccaagaa cgtgcagacc cacgtcgatc agctgtccaa
    23881 gaaggactac ttcaaggccg aaggcggctc aactcccgag tacgtggtgc tgtttctcgc
    23941 cagcgaggcg ctggccgcgg aggcctacaa ccagctgccc tcgctgctgg agtacgccgc
    24001 agagcgcggc atcatgctgg ccacgccgag ttcgctgatc gcgacgctga aaaccgcagc
    24061 cttcacctgg aagcaggtgg cgctggccga gtccgccaac gaagtgttca acctcggccg
    24121 tgagctgtac gaccggctcg gcacgctggg aaagcacttc gacaaggtcg gacgctcgct
    24181 gaccagcgcg gtgggcgctt acaacgaggc ggtgggctcg atcgaaggac gggtgttccc
    24241 gaccgcgcgc aagctgcgcg agctcaagct gaccgacaag gagctaccca gcgtgagcgc
    24301 gtccgaggct gcggtgcgtc cgctgacgag caacgagttg gtcgaggacg cggctggcgt
    24361 ggccccgata atcggacgcg cgaagctcga agaactcacc cggccacagc cgacgctgga
    24421 cgaactcgta gccctggagg gaccacgggc gaaaggcgtg accgcgcaga ctgcctagta
    24481 ggagcctgct gaacgcgtca cgagaggcgc ccccataccc gggccgggcc agttgccgga
    24541 cgggcgtgca gggcggcctc ggcccgggtg gccgcgccga gcttggtcag gatcgccgac
    24601 acgtggacac tgacggtctt cggactgatc cgcaactggt cggcgatctg gcgattcgac
    24661 cagccgcgcg cgaccaggtc gagcacctcc cgctcgcggg cggtcagcgg atcgtcggcg
    24721 ggcctaccgg gcccagaccc ggcacctatc ggtccgagga tcgcgcgata accggccgtc
    24781 tcggccgctc gctgaaccct cgcaagcccg gcccggtcgc cgccggcgat cgccgtccgg
    24841 gcctgcacgt accagcttgg ccggcgcacc ggggaagtcc gccagcgcgg acgcgaccgc
    24901 cgaccacgcc tcacccgcgc gggtgagttc ggcgcgtgcc gcggcggcca gcgcgcaggg
    24961 atagccgtcc gcagggaggg cgccgagcag ggcggccaga cgctcggacg cgatcctccg
    25021 acagcaccgc ctgcgcggcc agaagttgcc gcgtcgccgc ctcaggctcg ccggcccgcg
    25081 ccctctaccg gcgacggtaa ttgtgcgcac cgacggcgcc cccacgccgg ccggcgcgaa
    25141 tcagccggcc gtgccggcag cagtcggatg gtgggtccag tgatggtcgg tcaggaacac
    25201 atcgatgcgg gcgcggagcg agttccacca ctgggcgatc ggcagtggcc catcggctgg
    25261 gctgtcgccg gccagttggt tgcactcgcg gatgcgacgc attcgctcgt tctcgacgtt
    25321 cagctggctc aactgctgtt cgtacagccg ctgcgtcacg tagctatcca acatctgcgg
    25381 gcctcccccc gaaccaggtc tttacgactg ctccagcggg tcgcctcaga gggtgtcccg
    25441 gcgtggggcg aatgccctag ctgggatacg cagtgctggg taagcactcg ccctaggcga
    25501 gtatctcttc accggaatcc ggatcgaccc ggctcccacc aagcgctgcg gcgtcatcgc
    25561 atcgccgcgg tgccacaatg agccggtgac gatcgatgcc aacgcggtgg acgcggccgc
    25621 gcaacgcctc gcgggggtgg tcactcgtac cccgcttcag cccaacgatc ggctcagcgc
    25681 cgcgacgggt gcctcgatct ggctcaagcg ggaggacctg caacccgtcc gttcgtacaa
    25741 gatccgcggc gcatacaacg tgatcgcgca actcgacgcg tccgagcgcg ctgccggcgt
    25801 catctgcgcc agcgccggca accatgccca gggagtcgcc ttcgcgtgcg cgcgcctcgg
    25861 catccgcggt cgcatcttcg taccggccac caccccccgg cagaagcggg aacggatcat
    25921 cgcgctcggt gagggtcgcg tcgaactggt cgtgaccggc gacacctacg acgaggcggc
    25981 cgcggcgtcc ctggccgaag ctgcgcggac gggagcaagc gtcgtaccgg cgttcgacca
    26041 ccccgacacg atcgtcggtc aaggcactgt ggcgaaggaa atcgtcgagc agctcggccg
    26101 cgcacccgac gtgctggtcg ctccggtggg tggcggtggc atgatctccg gctgtctcgc
    26161 atacctttcc gagcggtatc cacagaccct ggtggtgggt gctgagccag ccgcagccgc
    26221 gtccatggct gcggcggtgg ccgccggcca gccggtgcaa ctggatcgga tcgacacctt
    26281 cgtggacggt gccgccgtgc ggcgagcggg cgacctcacc tttgccgagg tgcagaagca
    26341 cggcccacaa ctcgtcgcag tgcctgaggg ccggatctgc tccgagatgc tgtcgctcta
    26401 ccagaccgaa ggcattgtca ccgagccggc cggcgcgctg gcggcggcgg cgctcggtct
    26461 gtatcagccc gagccgggcc agaccgtggt tgtgctggtc tccggcggca acaacgatgt
    26521 ctcccggtac gccgagatcg tcgagcggtc gctggtctac gagggccgca agcactactt
    26581 cctggtgaac ttcccgcagg aaccgggcgc gctgcggcgt ttcctggacg aggtgctcgg
    26641 ccctgacgac gacatcgctc tgttcgagta cgtgaagcgc agcaatcgcg agaccggtcc
    26701 ggcactggtg ggcatcgaac tcggggatcc caactcgttc gctgatctac tgctgaagat
    26761 ggacgcaagc ccgatcacgt tcgaagtggt cgcgtccgac agtccgttct atcgcttcct
    26821 ggtttagctc agatccactg attgcggtgc gagatcccgg cctacgccgg gatgacggtg
    26881 gacgggtcgc tgcgagatcc cggcctacgc cgggatgagg gtggagccgg gcttgctgcg
    26941 gctggtcgag cttgtcgaga ccacttggca gggtctcgac aagctcgatc acccgagagg
    27001 ttcggggtcg ttcccgatca ggccaggccg ctagccttca accactcggc ggcaacgtcg
    27061 gctgcgtccg ccttgtcgac gaccaccttg gtcaccagcg tggccaggga gtcggtgtcg
    27121 agcttggccg agaccgcgtt gagtgcggcg gacacctcgg gcgtggcctt cgacttggtc
    27181 agcaccggga tcacgttctg cgcaccgaag aagctcttgg tgtcctccag cgagacgaag
    27241 ttgttcgcct tgatcgccgg gtcggtggtg aacaggttcg ccgcctgcac ctggccgttc
    27301 ttcagcgcct gcacggtcag cggaccggcc acgtccagcg gcttgaactc cttgaactcc
    27361 agcccgtagg tcttcttcag gccgggaatg ccagcggcgc gttccttcca ctccggcggg
    27421 ccaccgagca ccagttcgcc ggcaaccggc accagatcct cgatggtctt cagggagtac
    27481 ttgtccgcgg tctccttggt gaccgtgatc gagtccttgt cctgcgcgct ggacggctcg
    27541 agcgccacga ggttctcggg caacgcggcc ttcaacgagg ccaatgccgt cgcctcgtcg
    27601 gacgtgtccg cgtccggatt gaggtacttg gcgaaagcgc cggtgtattc cgggatcagg
    27661 tcgatctcgc ccgacttcag cgcaggcacg tacgtctcgc gcgaggcgat gttcagtttc
    27721 cgggtcactt gaatggactt ggcttcgagg gcgcccgcgt acagctcagc gagaagctcg
    27781 ttctcaggga agttcgccga gccgacgacg atcgccggtt gcgcagccga gcttccggcg
    27841 ggcgcggacg aagtcgacgg tgcggcagcc agcgggtcgc cggacgcgca cgccgtcagc
    27901 gcgatcgcgg cgatggtcga ggcgaggata gtggtcagtt tcttcattgg atggtccttt
    27961 gttcaggtgg atgggatgag ccggcggatc gagcgacgac atcgtccggt ggcccttggt
    28021 gcgctcagtc ctggcgaaac caccaggcgt tggactccgg cgaagacgag atcaaccaac
    28081 accgccagta gggcgaccag cagcgcccca ccggccatct ggccgtaatc gcggtaggcc
    28141 tggccgtcga tcaggaaccg gccgagacca ccaacactca ctgaagccgc gatggtcgcc
    28201 gtggccacca cctggagcgc ggccgagcgc aggccgctga acagcagcgg caacgcgcag
    28261 gggatctcca cgtcgaatag caactgccgt gggctcatgc ccattccccg ggccgcgtcg
    28321 cgagcggacg gatcgacgct ctccacgccg gcgtacgcac cggcaaggat cggcggaatc
    28381 gcgaggatca ccagcacgaa gatcgccggc acgaggaaag cgatgttgcc ggccagcagc
    28441 ggcccggcca ccagcacggc cacgaacaac aggccgagcg tgggcaccga acgcatgccg
    28501 ttcaccaggt tcaccaccag cacccgcccc ttgccggtgt gcccgatcca caggcccagc
    28561 ggcactgcga cgactgcggc cagcaccagc gtgatcgcgc tgtagagcag atgttcgaga
    28621 gttcggacgg ggatcccgtc cgcgccctgc cagtgcgcgg ggtcggccag ccactcccac
    28681 aacaggttca tgccgccctc cccgctcgtt gccacggcgt taggacgcgg gtcagccaat
    28741 gaatcgccaa gtcgagggca accgcgagca ccatgcaggc caggattccg acgatgatcg
    28801 ggtcgaggaa gtttcgggcg aacccgtccg tgaacagcga acccagctgc tcgatgccca
    28861 gcagcgcagc cacggagacg atcgagacat tgctcaccgc ggccacccgc agcccggcgg
    28921 caatcaccgg caccgcaagc ggcaggtcga cagtgaagaa acgccggacg cgtccgatgc
    28981 ccattgcggt cgccgcctgg gtgacctcat ctgggaccgc cgcaagcccg tccgccaccg
    29041 tgcgcaccag cagcgcaacc gtgtagatcg tgagggccac cacgacgttg agcgggttca
    29101 ggatgccggc gccgagcacc agcggcatca ggatgaacag cgccagcgac ggaatcgtgt
    29161 agagcaggcc ggtgccggcg accaggggcg tccgcagccc acggtaacgc cgggccagcc
    29221 aacccagtgg gattgccaac atcaggccga tgaccagcgg aatcaccgcc agcaccgcat
    29281 gtgtcaccag cagcgagccc acctcgtcca ggtggtcgag gaaccacttc atgtcgcatc
    29341 ccgatcgcta ggcctcggct cagggagcgt ctggtcgcgc tcaatcgccg cgagaacgtc
    29401 ctgggcgcgg acggtgccga ccagccggcc gtcggcgtca gccagcacgc cgcggcggct
    29461 gggagccgaa agggccgcat ccagcgccgc acgcagcgac ccgcccgcgc gcgctaccgt
    29521 gccgccacgg tgcagatcgt cccggtcgat gggcccggcc agcccggaga gctccaccca
    29581 tccggccggt gcgcccgcct cgtccaccac cagcagccaa tcttcgccgc cggccgcctg
    29641 ccgcgcgtcc gccacgcttg ccccgagcgc gaccgttggc tcacggcgaa tcggtaggtc
    29701 gggcgccgct tggaacgcca gcccgcgata gccgcggtcg cggccgagga agtcggccac
    29761 gaagtcgtcg gcggggtccg ccagcaagcg ttccgggctg tcgagctggg ccagcgtccc
    29821 gccgacccgg agcacggcca cctggtcgcc gagcttgatc gcctcatcga tgtcgtgggt
    29881 gacgaacacg atggtcttgc ccaactcggc ctgcagccgc aggaactcgt cctgcagttg
    29941 atcgcgaacc accggatcga ccgcactgaa cggctcgtcc atcagcatca ccggcgggtc
    30001 ggcggccagc gccctggcca cgccgacgcg ctgctgctga cccccggaga gctggttcgg
    30061 gtagcggttt gcgagttccg gggcgaggcc tacgcgctcc agcaactcga gcgcctctgc
    30121 gcgggccttg gcgcgtcccc agccgagcag cagcggcacg gtggcgatgt tgtcgacaat
    30181 ggtgcggtgc ggaaacaggc cgccgctctg gatcacgtag ccgatgccgc ggcgcagttc
    30241 gttcgcgttc aggttcgccg tgtccctgcc gtcgatggtg atcaaacctg aggtgggctc
    30301 gatcatccgg ttgatcatcc gcagcgaggt ggtcttgccg cagccggatg gccccaccag
    30361 caccgtgatc ttcccggtcg gcgcggtcag ggtcagcccg ccgaccgcag tggtgccgcc
    30421 cggatagacc ttggtgacat cagtgaagct gatcatgccg gagcacctcc tggtcgtggt
    30481 tcgacaccgg agtccggaac acgggcgcgc gcatctcgga gaggtcgtcc cctcgacgct
    30541 ccctgcgtag cgcttcgatc acccactcga tcacctcagc ggtcacctgc gtccgtccgg
    30601 tcacatcaat gatggccgca cgcaaccgct cgacgggcga accgagctcg gcgggatgcc
    30661 gaagctgatg catcgagatg accgtcgcgg ccagcagcgc ctcgacggcc aacagttgtt
    30721 ccaccacgtc cagcacgccg tctgtgacgc gcacggacga gtaggccatg gacgagtggt
    30781 cttccacccc gtcgcccacc ggcgaaccgg tcagcgagat cggcgacgcc gcgtgcttga
    30841 cctgggccag cagcgcggag gcggtttggg cgaggatcac gggcgtcacg taccccgtcg
    30901 cggacgtgac cgcctgcacc tggtcaacca ggggcagccc ctgtcgaagt tggcgcacca
    30961 gcattgccac ccggcgctcg gcgagcttcg cgacgtgggc cagcgcgatc cgcagtgcgt
    31021 cgaaccggat cgccaggttg gtgatcgaga agttgccgtt ggaaatgaac tcgcccgact
    31081 cgatgtccag gaaggggttg tccggcgcgc agttcagttc gacggtgagc acgcccgcca
    31141 gttcgtccag ttcgtccagc agcgcaccgt gcacctgcgg caccgtgcgc agcgacaccg
    31201 cgtcctgcac cgaggaggac gcgcggacgc cacgagccag gtcgccaccg gccagcgcag
    31261 tgcggatccg gcttccactg gcctgttgcc cgggcagcgg acgcgcggtc aacgctcgcg
    31321 cgtcgaacgg gctgaggttc gcacccagag cctccaccgt gagcgcggcg accagatcag
    31381 caccctcggc caggaggacg gaccgccgct gggtgagcgc agccgtagcc accgagagcg
    31441 cgttcgagcc gaccagcgcg aggctgtcct tggcggcgag ctggacgggc gctagcccgg
    31501 ccgccgcgag cgccaccgcg ccggggacga tctcgtcctg gcccggcagc caggcacgac
    31561 cggagccgat cagcaccagg ccgatcgcgg ccatctggga gaggtcgccc gcgcccaccg
    31621 agccctggtc gggaatgacc gggatcacgt ccagccgaag taggtcgaag agtccgcgga
    31681 agagtgcctc cgaagccccg gaagcacccc tggcaagggt gttgatccgg gccagcaggg
    31741 ctgcgcggcc ttcggtgcgc gacagcggct cgccgatcgc cgccgcgtgg ctgacgatca
    31801 cgaagcgctg gaaatcggcc atcagctcgt ccgggacctc gctgtcgcgc agcgggccga
    31861 gaccccggtt caacccgtag gtcggcacgc cggtggccag cgcgtcgtcg accacggccc
    31921 gcgctctggc caggcgctcg agcacttcgt tcccgctcag cgcgacggtt gcgtggccga
    31981 tcgcgacgtc ggcgacctgc tcgggggtca gttcggagcc gatcaccacc acccctggct
    32041 ggtggttcat ccggccaccg gtggtcgggc aggacggggc gtcccaccgg ccgaggtgac
    32101 ttacgctcgc tgcacttggc gaagggactg agtcggttcc aggtgctgtg gtcccgacag
    32161 gctcaaccac gcgagggctg gggctgatca gccgaggggt ggnnnnnnnn nnnnnnnnnn
    32221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    32281 nnnnnnnnnn nnnnnnnnnn nngaggggtg ggatcggcga gagcggtcat caggcgtccc
    32341 ccgcataggc ggcggtgccg ggacgcaggc tgggatcgag gtggctgagg aagtcgatgc
    32401 ccatgatctg ctcgcactcg cgcagcccca cctggtcgat gctcgaactc gacaccggca
    32461 gcactccggc cttcgcaacc agcaggacaa cgtcctgacc accgcggccc gcgtccttca
    32521 cggccaggcc ctcgccggta ccgcggtcga ggatgacgat ggtgtcgggg taggtcgcca
    32581 ctcgtccgcc gtcgttgtcg accgccatgt actcgttgag gtacgggacg gtgtggtcgc
    32641 cgatccggaa ggtgccacga tcccagcccc cgatggtcct ggttcgcttc tcgatccgca
    32701 acggaccggc gtccaccacc tgcgcaccca ggcgctgggc gaccgcctcg accaccgcgt
    32761 ccgcattccc ggacgcctgc gccgcggcca ggtcgcggcc aaggtcgagc gcgagcgaga
    32821 tcgagccgtg cgcgccgttg gccttcaccc agtcaagttc gaccggattg cgtgcggacg
    32881 cgatgaagcc accgacccgc accgagatgt cgcgcagcac gtcgtcgacg gtgttcagat
    32941 ttccacgatt gaccgactgg aaatgcccgt gcagttcgcg gttgccgccg gagaccacct
    33001 ggatggactc gtagccggcc cgctcgccca ggccaagtcc gccgagcttg ccggtcgggt
    33061 gggcgcgcac gtcgccagcg ctgtcgagca ccaacacgcc cagcacagcg gactggatcc
    33121 acccgttcac cgttgtggaa tacccattct gcgcagtcat caccgcaccg atgggcacct
    33181 ccgccacccg gatcagctcc tgcaacgcgt gcacgtagtc gaccggacgg atctcccaat
    33241 ccttcgctgc cggagcaccg atcgccgaga cggtggctac ccaggtcgct tcaggcaact
    33301 catcgatcga ggccagcacg ggccgctcga ggctggtggc gagcgttccc atcagctcgc
    33361 cgtggtgctt ccagccgccg ccaccgcagg ccaggatgcc gccgccgagc accgcatggc
    33421 gtgcgtccgc actggtgagt tctctcatcg gttctcccta acgctggctg aactgtcgtt
    33481 ccaatgaccg gacgtacgcc gggatgacgg tgaagctgga cgcacccagt tgaccggcgc
    33541 ctccccggtc tcgccgccgg cggccacgct ggcctcggcc tcggtatacc ctgcggaggc
    33601 gtggcgcagc acgccgagac cggtgtcagc gtcgagggcg cgacgcagcc ggacggccgt
    33661 ctcggcacgc ccgtccgcga cgatcgagac accggccgac tgcatccaac ccgagtaccc
    33721 gccacctccg gagtggatgg ccaccaggtc ggcaccggta gcggcgagaa gcatcccgtc
    33781 aaggagcggg aagtcggtgg acccgtccga gccgtcccgc atgccttcgg tgccgattcg
    33841 cgggtgggtc atggccgcgg agtcgagatg gtcacgactg aacagcaccg gcgcgaccag
    33901 cgtgccgtcc gcgaccagct cgttgaccgc caccgcgaac gccgagcgtt cgccatgtcc
    33961 cagccagcag gacctggctg gcaggccctg ctgcggcacg tactcacgag ccagttcgat
    34021 ccagcgcgcg acctcagggc gccgcgggaa cgaacgggca gcgaggtcat ccagcaccag
    34081 ttggtctgct accgaacccg agagcgccac ccatcggaac ggcccgatcc cccgggcgaa
    34141 cagcggcctc agataggcct ccatgaagcc cggaatgcgg ttcacttgat cggtcgcggc
    34201 cgcaccgaga tgttcgatcg cctgcacccg caggttgttg ccgttctcga acaccaccgc
    34261 gccagcggtc gcgagggcca gcatggcctc cacctgcgca gcgatggacg cacgcgcgtc
    34321 gcgctccacc tgccgaggct gggtctcccg aagcgtccgc cagccggcga ggtcgtagcc
    34381 gcgcggcagg tagccgtaac gggcgtcgtg ggcagctgtc tggtcggtga cgatgtcggg
    34441 gacgacccca cgcgcggcga tctctgcgaa gacgtccgct gcgttggcga gcagcgcgat
    34501 cgcgacagtc tccgctgccg acttggcctg atgcagtgcg gccagggcgt cgtcaaggct
    34561 ttcggtgacc aggtcgagcg ctccggtagc cattaggcgt tcggccttca cggggtccac
    34621 ctcggccgtt agggaactca gaccaagcat gcgtgcgctg atcggctgcg cggagcccat
    34681 gccgccgagg ccggaggtga gcacccagcg gccggagagg gcgtccgcct ccgatccggg
    34741 tgcggccccg aagtgcgtcc gagcgatcgt gccgaacagc tcgtaggtgc cctgcagcac
    34801 tccctgccgg ccgatgtact gccacgccgc cgcagtgagc ccaccccaca tcgtctggcc
    34861 ggcggcggcg cgagcgtaga agcggtctgc gtccgaccag cgtccgactg tgttgttgac
    34921 cgccgagagc accagcggtg cgggcgtgcc ggtgctcagc acgccgaccg gcgcaccgga
    34981 ggcgagcacc aaggtctcgg cttcgtccag ttcgagcagg gcgttgacga tggcgtgggc
    35041 gtccggccag gagcgggccg ccttaccgag ggacgcatac acgatcaact cgtcgggaga
    35101 ctcagcgact tcgaggacat tctcgaacat ccgcagcaga ccctccgcac gccagttccg
    35161 ggcgcggagt tcaccgccgc gctgggcgac gatcggcacg ccggggccgg cgaggatgct
    35221 gctgggacgg gcggatgcgc cgggactaga aacggtgacc tccggtgggc ggagccacag
    35281 tgccgaaata tcggctggag cggctgctat gaacgattcg cgaggctcga cctagtggtc
    35341 ggcttggtgg gttggaagct gcgccctcag caacagtccg agcggtgggt gccacgacaa
    35401 cacatcgccc gacaacacat gcgacaacaa cgttcgtgca cagcccacct ccctaaattg
    35461 aatcgtcaat cggcatcgtg gggagacatt agcatgccct cgaacccgtc cctgaggaga
    35521 cgatgggcac atgcccgtcg acaagctcag gaatcgtctc tgatcgggtg gtcgagcgcc
    35581 gaggagcccg tcagtcgccg atggtcttgc ggcgcaggtc cttacggagc tccggcggca
    35641 gggcgaaggt gaggttctcc tcggtcagcc gctcctccac cgcgggcggg aatccgcgct
    35701 cggtgaggaa atcaagcacg ccctgtacca gcgcgtccgg caccgaggcg ccagaggtga
    35761 cgccgactga gcgcacgccg tccagccagg cttcgtcgat ctcgctggcg ttgtcaacac
    35821 gataggaggc cttcgcgccc gcctccagcg cgacctccac caggcgcacc gagttcgacg
    35881 aattcctgga cccgacgacg atcaccaggt cggaatgcgc ggcgatctgc ttgaccgccg
    35941 actggcggtt ctgggtggcg tagcagatgt cgtcgctggg cgggtccatc agcgccggga
    36001 acttctcccg caggcgtccg accgtctccc aggtctcatc gacactgagc gtggtctggc
    36061 tcagccaggc gaccttgctc gggtcggcaa gttcgactcc cgccacatca tcggggtgct
    36121 gcaccagcgt gatgtgctcc ggagcctcgc cggtcgtccc ctcgacctct tcgtggccgg
    36181 cgtgcccgat cagcaggatc tggatgccct ggccggcaaa ccgcttggcc tcgttgtgca
    36241 ccttggtcac cagcggacag gtggcatcga tcgtcttgag cccgcgatcg gcggcctcgg
    36301 catgaaccgc cggcgaaacc ccgtgcgcgg agaagacgac cgttgcaccc acgggcacct
    36361 cggccagttc ttcaacgaag atcgcgccgc gggcctccag gtcctcgacc acgtgccggt
    36421 tgtgcacgat ctgcttgcgc acgtagaccg gcggaccgta gacgtccagc gctttctcca
    36481 cggtgaccac tgcgcgatcc acaccggcgc aatagccccg gggcgcggcg accaccactc
    36541 gcttggcatc tggcatgggc acgattctac gtcgcgtgac cggtggcccc tgacggaccc
    36601 tcgcgtgacc gaatgggaca gacctgacac cagccgacga ctcgtcgcgc tggtcggcga
    36661 tccggcgcgt cgactggcag ctttgtccca ttcgacaccg gcgtacgcgt tcggttcgac
    36721 accggcgcgc gcgttcgggc cgatccgacc ggtgtcagca ccgcccgcta gcgtgagcat
    36781 gtgccaatgc cgagttcgcc ggagcagccc caaccgctgc gagctgtggt cgccgcgacc
    36841 aagcagtggg tcgaacggct gaacccgatc tgggtggaag gccaactgat tgagatcaag
    36901 cgccgcgccg gatcggtaac ccagttccta accatgcgcg acaccctggc tgaagtctcg
    36961 gtgaccctct ccacctcact cgccgtgctg gacgccgccg gtcccctaac cgaaggcacc
    37021 gtggtggcag cgtgggtgcg tccgacggtg tggagcaagt cgggcagcct gaccttcgaa
    37081 tgccgcgagc tgcgtgcgtc cggggagggt cggctgctgg ccgcgatcga gcagcgcaaa
    37141 cgcatgctgc aggccgaagg cctgttcgac ccggcgctga agaagcgctt gcccttcctg
    37201 ccgcgaagga tcgggttgat caccggaacg cagagcgcgg cggagcgcga cgtggtcgag
    37261 aacatcggac gccgctggcc ggccgccgtg ctggaggtgc ggcacacgct ggtgcagggc
    37321 gcagctgcgg tcgagcagtt gatcgatgcg gtccgggcgc tggaccgcat gccggaggtg
    37381 gacgtcatca tcatcgccag gggcggcggc tcgttggaag acctgttgcc gttcagcgac
    37441 gaggcgctgg tgcgcgccgt tcacgcgtgc cggacgcccg tggtgagcgc gatcggacac
    37501 gagaccgaca cccccatcct cgatctggtg gccgacctgc gcgcgtccac gccgacggac
    37561 gccgccaaac gggtggtgcc ggacgcgacc gcggaagcca cggcgatcgc gcacgcgatc
    37621 ggccgggtgc gacaggccgt gctgaaccgg gcgggcaccg aaacgcgtcg tctcgccgaa
    37681 ctgcgcagcc gtccggtgct gcgcgacccg tccgcgtcgc tggaggggca tcgcgaacgg
    37741 ctggcgcacc aggaaacgcg gctgcggcga gcgatcgagg cgcggctccg cgaggagcag
    37801 ctgggcctcg gacacaccat cgagcgggtg cgctcgttgt caccccgcgc cacgctaaac
    37861 cgcggctacg cgatcctcgc ggacgccgat ggcggcaccg tcgatcgagt cgcggccacc
    37921 agcgtgggcg ccgcgatcac cgcccgtctc gcggacggac gcctcgaact gaccgtcacc
    37981 gatgtcgaac cccaggagca gccatgacca agctcagcta cgaagaggcc cgcgaccagc
    38041 tggtcgagat cgtccggcaa ctcgagaccg gcagcgtgcc gttgtcggag tccatgaagc
    38101 tgtgggaacg cggcgaggaa ctcgc
//
LOCUS       DAOPRQ010000017        37931 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_839_length_37931_cov_11.695295, whole genome shotgun sequence.
ACCESSION   DAOPRQ010000017 DAOPRQ010000000
VERSION     DAOPRQ010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 37931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37931
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_839_length_37931_cov_11.695295"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(83..1816)
                     /locus_tag="PKJ61_03465"
     CDS             complement(83..1816)
                     /locus_tag="PKJ61_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOC12917.1"
                     /translation="MRTVISDETKIRYRYPARAIGLVGAVLMIVSIFLPWAYGHGALD
                     DVGYAGAPSPLQLFFVLLPVLLLLLLAAPLVGKSRLGRFAKVVAWNTTAKTAAIGATA
                     TVLVALAAIAIELSGLVNIEIGGWLALIGGVVAIAATLFLPDSRTPSLGRVKSPKWLQ
                     IVGIALLMGSALFAAAFALNQNDAGAFLTFAAFLVSIVLVLRQFGVFGWLGVAAANNN
                     RVLVFSAFLVAFAFPFTQNGSDANMSIASQVLIFAAAALGLNIVVGLVGLLDLGYIAF
                     LGAGAFTAAILSDSAFSHYAGIKPPFLVTALIAGAVASILGLIIGAPTLRVSGDYLAI
                     VTLAFGEIFRITMNNLDGNDGPNITNGSNGISGIPDLEFFGFNFGDPHVLLGVPIGRF
                     ANYYWLMLIVIALIIVVFMNLNYSRIGRGWVAIREDELAAEAMGVNTFALKLLAFAGG
                     AFLAGIAGAVKAHHDVSVTPDQYVFLESAFLLAAVVLGGMGTVMGVLVGAAILKLLPE
                     KLRFFADYRLLLFGLLMVFMMQFRPEGIIADERRQLEFHDEDEELAEEIEEELNLRHA
                     HFNPEQKGFEL"
     gene            complement(1813..2760)
                     /locus_tag="PKJ61_03470"
     CDS             complement(1813..2760)
                     /locus_tag="PKJ61_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOC12918.1"
                     /translation="MDLFLQQLFNGLSLGSLYALIAVGYTVVYGIVQLINFAHGEIFM
                     MGAFGSFATWMLLGAPTNWGVSASLFVLLPLMILGGVIVSISVALLTERFAYRPLRNA
                     PRLAPLISAIGVSVFLQEVVRLFFGRIPGMPDAKSAVPFPAIPGISGAIIHVGGVTIQ
                     MSALFTVGALVVCTAFLWFFVNRTKTGRAMVATSQDRDTAQLMGINVDRVIVAAFAVG
                     AGLAAVAGVAHGLRYGNIDFRMGFMAGLKAFTAAVLGGIGNINGAVVGGLVLGLVEAL
                     ASNYLGTTAWKDVWAFILLILVLVFRPQGLLGAKVVDRA"
     gene            complement(2837..4045)
                     /locus_tag="PKJ61_03475"
     CDS             complement(2837..4045)
                     /locus_tag="PKJ61_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HOC12919.1"
                     /translation="MKTQSFKLAGVALVVASLAFTGCGSRTEASTGGGGGATKVAKIG
                     VIVPLSGGLSAMGLGIRNSVELAIREANDKNVIPGWKLELDAQDDEAKPDVGKNAATK
                     LASDDAVVGVVGTLNSSVAQSVIPVLDAAKIVQVSPANTNPALTKGSDLANPKRPNAN
                     YFRVCTTDAVQGPFAAQYLLGTGIKKVATVHDKKSYGQGLAEAFAEAFKAGGGTVVAA
                     ETINPDDSDFSAVITKVKAAGPEALYYGGEYPQAGPLSKQMKAAGLNVPLMGGDGIYD
                     ANFIKLGGTDGDLATSVGAPVETLASAKAFVEAYKAAGFPDGFSAYGAYGYDAANAII
                     AALKTSLASATDAKSARQATIDAIGKVSFDGATGKVGFDEFGDSVSRVLTVYKVTGGA
                     WKAEKTGELQ"
     gene            complement(4187..5398)
                     /gene="hemW"
                     /locus_tag="PKJ61_03480"
     CDS             complement(4187..5398)
                     /gene="hemW"
                     /locus_tag="PKJ61_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HOC12920.1"
                     /translation="MPSQPPDGLPAPADGSLPAAALAGLAERRLSLYLHVPFCATRCG
                     YCDFNTYTAAELGAGPGAGQDAYLAAAEAELELAVRVLGCPPPLQTIFFGGGTPTMLP
                     AAALAGLLTSARSRFAVAPDAEITTEANPESVDREYLDRLVAAGFNRLSLGMQSARPG
                     VLAVLERRHTPGRVRQVVEWARAAGFASVSVDLIYGSPTETIDDWRTSLEAALSFAPD
                     HVSAYSLIVEPGTRLAAQIGRGQVPAPDEDLQADCYLLAEELLTGQGFSAYEVSNWAQ
                     PGHASRHNLAYWVGDDWWGIGPGAHSHVGGVRWWNVRHPRDYSARLAAGSSPAQAREL
                     LTDEQRRVERVLLELRLSQGLPVSVLTSSEAGRVPGMLASGLVRLEAGNLRPTLRGRL
                     LADAVIRDLLD"
     gene            complement(5401..6216)
                     /locus_tag="PKJ61_03485"
     CDS             complement(5401..6216)
                     /locus_tag="PKJ61_03485"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: protein motif:HMM:NF005876.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HOC12921.1"
                     /translation="MDAEIGIIGGSGFYTFLSNPERVEVDTPFGTPSAPLTVGEVAGR
                     KVAFLPRHGVNHEYPPHKVNYRANLWALRSLGVRQVIAPAAVGSLVAEHGPGAFVVPD
                     QVIDRTWGREHTCYGEVGGVVHTAFADPYCPRGRAAVLASADETTPLVPVGTLVVING
                     PRFSTRAESRWHAAAGGTVVGMTGVPEAAIARELAMCYTTVCMVTDHDAGVEAGEAVT
                     HAEVMRVFAGNIDRLKTLLVRVIAALPAAESDETAACLCRRVHDGQQLPFVLP"
     gene            complement(6695..8530)
                     /gene="lepA"
                     /locus_tag="PKJ61_03490"
     CDS             complement(6695..8530)
                     /gene="lepA"
                     /locus_tag="PKJ61_03490"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015582921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HOC12922.1"
                     /translation="MPTPSPGKTPPALIRNFCIIAHIDHGKSTLADRMLQLTGVVDAR
                     SMRAQYLDRMDIERERGITIKSQAVRMPWQVDGTDYILNMIDTPGHVDFTYEVSRSLA
                     ACEGAILLIDAAQGIEAQTLANLYLALEADLHIVPVLNKIDLPSAQPEKYAAEIAHLV
                     GCDPDDIFRVSAKTGEGVKELLDHVVADIPHPVGDPDAPPRALIFDSVYDTYRGVVTY
                     VRVVDGELSHRERVLMMSTKATHETLEVGVISPEPVKAQSLGVGEVGYLITGVKEVRQ
                     SRVGDTVTAASRPAKQDLGGYRPPHPMVYAGLYPIDGADFPELREALDKLQLNDAALV
                     YEPETSGALGFGFRVGFLGLLHMEIVRERLEREFNLDLISTAPNVVYRVVMEDGTEHE
                     VTNPSEYPDGKIAEVHEPMVRATILAPAEYIGTIMELCQARRGEQTGMDYLSEDRVEI
                     RYRLPLAEIVFDFFDALKSRTKGFASLDYDEDGEQVAKLVKVDILLHGEPVDAFSSIV
                     HTDAAYSYGVAMAKKLKELIPRQQFEVPIQAAIGARVIARETVRAMRKDVLAKCYGGD
                     ITRKRKLLEKQKEGKKRMKMVGRVEVPQEAFVAALSTQETSEKKK"
     gene            8814..9074
                     /gene="rpsT"
                     /locus_tag="PKJ61_03495"
     CDS             8814..9074
                     /gene="rpsT"
                     /locus_tag="PKJ61_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOC12923.1"
                     /translation="MANIKSQKKRILTNEKARLRNKAVKSGLRTNVRKFNEAVAAGDS
                     EKAQELAKTATRSLDKAASKGVIHKNQAANRKSAIAAKAAAL"
     gene            complement(9133..10110)
                     /gene="holA"
                     /locus_tag="PKJ61_03500"
     CDS             complement(9133..10110)
                     /gene="holA"
                     /locus_tag="PKJ61_03500"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HOC12924.1"
                     /translation="MPETAFATALLVTGPESLLAERAVADFVGRATAERPDAAVVRLQ
                     AGELDAGALAEAAGGSLFASASIVVVSNLAELPAELADHVVALATDPGDDLALVLVHG
                     GGVKGKALLDRLKKLRVPAQDFAAIKPWDLPQFVAGEVRRCGGRVDARTAGALIEAVG
                     SDTRALAGAVRQLLDDSPDRLLTEVAVRRYFGGRAEVTSFAVADDVLSGRVDGALGKL
                     RWALSTGVAPVLITSALSNNLRSLGKYLDGRDARLRDAELARVVGVPPWKLKDLSRLS
                     RDWKPTAVAAAIRQVAIADAQIKGAASDPGFALEQLVLGVIECRRSERR"
     gene            complement(10158..10808)
                     /locus_tag="PKJ61_03505"
     CDS             complement(10158..10808)
                     /locus_tag="PKJ61_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC12925.1"
                     /translation="MIFNLRTRCRVISQGNLGIVVDGNGSAYLVADVDPRLPEGGFRI
                     TCRAGVPGLGVLRLDGRCGDAVCPIARPEINHLLQHHLDAVDPAEVTVVEVDLEAVTV
                     LAPATDLCRACAIPVALDAYASARSESWLLDVAGIVECIEREHQFDLRILVGALGYPN
                     APAAIISVTSMSSLRLDLVCMDADGVTAVTVPFGTRLDHPAEVPGWLAQAAGRPFS"
     gene            10798..10962
                     /locus_tag="PKJ61_03510"
     CDS             10798..10962
                     /locus_tag="PKJ61_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC12926.1"
                     /translation="MKIKGGIDGAVAQVGACSRTDCRHNADLECTADAVRVGAGADGM
                     AANCLTYEPA"
     gene            complement(11032..13299)
                     /locus_tag="PKJ61_03515"
     CDS             complement(11032..13299)
                     /locus_tag="PKJ61_03515"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HOC12927.1"
                     /translation="MASWAGAWAATSGVNWWAVGCGLLALALGLLAWRSRHWLRIAIA
                     VTMLVVVGTAWARVWLASVGPIPTWAADNAVVTITARVSGGQITDGGVGGPLWIATAA
                     LVDAEGRGDAWITGASVRISAGGTQLESWANLPAGSTVRALVRLSPAEPGESVGAWAR
                     ARASPTVVVEPGAVDQAVTSVRAGLRASVAGLPPDPRALVPALVVGDTSGMTEALQDR
                     FRATGLTHLTAVSGANLTLLLAALTWAAARVGVLGWWRRLLAVVGVAAFVLLCRAEAS
                     VLRAAAMGVVGLAALGWGGPRQGLRYLSWAVIGLLLVDPWLSRSVGFALSVCASAGIV
                     LWARPWAGVLQAWVPAWLAEALAVPAAAQLATQPIVSAISGQVSLVGLVANLVAGPLV
                     GPGTVLGFLAAGVSVASPPVAALLGWAAGGFAQSLCWIAEAGSALPGATLAWPATPLA
                     TAVMVVLCAGTLVLMPRLLRRPWLLVFAVAAQVLVLLRPVTTPGWPPTEWAVVSCDVG
                     QGDATVISIGEGQAIVVDTGPEPGLVDKCLDQLGIVDVPWLILTHLHSDHAGGVAGVA
                     SGRSVGRLLYSGITEPAANWHGVRAALAGNPEQVAVPGMVVAAGATTVEVLAVRPLVS
                     GSEVAGEDSAEQNDSSLVLRVTTPELRLLLSGDVEEAGQRNAVTRVADLSAEVMLVPH
                     HGSAHQDAAYLAAVHASVALISVGAENDYGHPAARTVDSVAGTGAQIFRTDRSGAIAV
                     SRRDGELTVTTQRSG"
     gene            complement(13510..14412)
                     /locus_tag="PKJ61_03520"
     CDS             complement(13510..14412)
                     /locus_tag="PKJ61_03520"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HOC12928.1"
                     /translation="MKVAPEPMMSEVVRARLEALLAEVEPRRLQPSGTEGWAPVDTST
                     AGPPRASPQDPGSFHLPSPGLGRPATGSNQGAADPPPPRRAANPPGHNGITQLRTRLL
                     DFGREHLAAVVVILLVGCAWTAYTMLQARSTPVAVAAVPSVVASPTPTPTPEPRLLVH
                     VLGAVRKPGLVDLPTGSRVADAIEAAGGLSASADPGELNLAALVADGDQLVIGTDADP
                     GGEVRGAGGSSGSAGSSGSGTISLNTATLAQLDTLPGVGPVTAKKILDWRDAHGRFSS
                     VGELQEVDGIGPKIYAEIEPHVRV"
     gene            14575..14874
                     /locus_tag="PKJ61_03525"
     CDS             14575..14874
                     /locus_tag="PKJ61_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016669301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HOC12929.1"
                     /translation="MAIDIDLNEKKTEGGGCGCGCGTRAGVPELQAAALPKVIRHGAI
                     IGGLTSMKSGEALVLVVSHDPLPLLSQLNRVAPDAFDLEYLERGPEAYRLQFTKR"
     gene            complement(14972..17464)
                     /gene="leuS"
                     /locus_tag="PKJ61_03530"
     CDS             complement(14972..17464)
                     /gene="leuS"
                     /locus_tag="PKJ61_03530"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020388456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HOC12930.1"
                     /translation="MRTPHEGDEVTEERIGYDAAAAQEKWQRFWEADGTFSPADDGTK
                     ERRYMLDMFPYPSGDLHMGHAEAFVMGDIVSRYWRLAGYDVMHPIGWDSFGLPAENAA
                     IARDAHPADWTYANIDTQARSFKRYGLSLDWSRRLHTSDPEYYRWTQWLFLRFFERGL
                     AYRKDSYVNWCPNDQTVLANEQVVQGLCERCSAVVTKRKLTQWYFKITDYAQRLLDDM
                     EQIEGKWPDRVLAMQRNWIGRSEGAWVDFRIEGREAPVRVFTTRPDTLYGATFFVVAP
                     EAAIADEICAPTARAEFEAYLEQTKRATEIERQSSERPKTGVFLGVHAINPINGERLP
                     VYAADYVLAEYGTGAVMGMPGHDQRDLDFARAFDLPVRIVLDSGDPHPAESGIATKGD
                     GTYVNAGPLTGMTSVKEAVAKTNEVLTEAGTGEAAVTFRLRDWLLSRQRFWGCPIPIV
                     HCPECGEVPVPDDQLPVTLPDLRGAALQPKGTSPLASATDWVNTTCPKCGGGALRDTD
                     TMDTFVDSSWYYFRYCSPNYTEGPFDVAEVAKWMPVAQYVGGVEHAILHLLYSRFFTK
                     VLADLGLVDFSEPFSRLMNQGQVINQGKAMSKSLGNGVDLGEQIDRFGVDAIRTTVVF
                     AGPPEDDIDWADMSPGSSLKFLQRAYRLAADVTSAPGAGFAAGDLAMRRVTHKAIAEI
                     TESIESHRFNVAVARIMELVNATRKAIDSGVGGADAAVREAVETVAVTLSTVAPYVAE
                     EMWELLGGAPSVANANWPVADPALLVTESVTCVVQVQGKVRAKLDVSPGISADDLTAV
                     ALADDGVQRALAGREVRQVIVRAPKLVSIVPG"
     gene            17808..18518
                     /locus_tag="PKJ61_03535"
     CDS             17808..18518
                     /locus_tag="PKJ61_03535"
                     /inference="COORDINATES: protein motif:HMM:NF014090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HOC12931.1"
                     /translation="MTITIPALEASEQSLSARLNWLRAGVLGANDGIVSTAGLVFGVA
                     GATADSLALLIAGLAGLVAGALSMAGGEYVSVSSQKDTETAALAAQSQRLSDDPEGEL
                     QRLADHYAGSGLSSGLARQVAEELTSHAALDAHARVFLHLDANERVSPWSAARASMIA
                     FTIGALIPLLAIVLTPQTLRLPLTVVAVLAALTLTGVVSARLGGARVWRAIVRNVLVG
                     SLAMGLTYLVGSLVGQLL"
     gene            complement(18478..19488)
                     /locus_tag="PKJ61_03540"
     CDS             complement(18478..19488)
                     /locus_tag="PKJ61_03540"
                     /inference="COORDINATES: protein motif:HMM:NF025200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4192 family protein"
                     /protein_id="HOC12932.1"
                     /translation="MNVSEPPARLAVRNIDDLVGLVPFLIGFHPAESLVVVVIESGQV
                     EVTARVDLAAVAEPGALDDLIGRLFDRFPRAVGWFFAYSADDSCAWEVLGACSGLVGA
                     TRVGRLLQVSDTVWRADHPDGPSGALNGTVTAAAAQAAVLGLPARASRAALAEGIAGP
                     PDAEIDHLLGMFERAAAALDRASASARARLLRRLLREPEPDLFAQARLALLVDEPAGM
                     LIALRALSRERASEQLELWTGVVRHCLLGYQAVPLGLLAMAAWQTGDGALQMVCLERL
                     DRIDPGLPLAAVIDKLNHEMVPPGAWPELRRPVLAWLAEWSDELADRVREAALPESRP
                     GR"
     gene            19615..20292
                     /locus_tag="PKJ61_03545"
     CDS             19615..20292
                     /locus_tag="PKJ61_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC12933.1"
                     /translation="MTSEPNRDPASQQPPASAPHPSAPPAGWAPTPLSGVTRPAVPRP
                     AAGGGPSAPLGGSNLPSTRIDEFAAPRSWWPLVVGVVAVLAAALIWASTTLRPALPSA
                     TKPSPTPAPTATAPGRPFVSPNERYSGRWEILQSEWTDSGVEVQIRISVDKGPLSYSF
                     VAFENNSVTASDPLPAAHSPRFSGYPIETGDTETGWLLFPLERGQATVILANAAGSQM
                     SALPIPG"
     gene            20489..21736
                     /locus_tag="PKJ61_03550"
     CDS             20489..21736
                     /locus_tag="PKJ61_03550"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOC12934.1"
                     /translation="MTAPLSIGIFTDDFYPESGGVSRSIQLQLQQLSQAGHGVTLFAP
                     NFQFTAPSECAWEGLPYWRLPGTPSYLCSLQTGRRLARRIAADHRLDVVHSQNERGSV
                     FLAAQVARAANVPQVHTFHSNYVGTHRTTPLVSGLNSFTYLDWSGRWLRRTAGRGNDV
                     TIRSSSDPDAGSDSRFANRDWRSLARLASAVDAFTSPAAYLVEAVVDASGGELDGRGH
                     VVASGVADAFVHAERKLPRGGRVRFLSCGRLGPEKRVDVILDAFERLGRDDAELHIVG
                     TGSAEGALRQQASRIRHGTVRFLGHFDDAALIAQEIADADVFVLASYRFDTQGMVLAE
                     AAAAGTPILYCDDRLSVGVSGENALLTGPSAAELAAGMAELADAPERLVAMSKASKRL
                     GATLTAEAMAERYLAVYRQVISG"
     gene            complement(21763..22725)
                     /locus_tag="PKJ61_03555"
     CDS             complement(21763..22725)
                     /locus_tag="PKJ61_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC12935.1"
                     /translation="MTASIPLAAPAWLFEQNIPLAILIVVFASFLFAGGSTIQHLAVS
                     RTVDKESENRSMGLRQVLALLTNRRWLLGLLTVGLGASLHIVGLMLAPVTVVQPVGIL
                     AVPWAVLLAAKIHHFRPTKVIWGAVALTILGIVVFTLFSASTAAPDTELPPARIIVSC
                     LIVFAAGGVLGWLGRFGSPSRRCLMWASGGSFFYGLSSALIKTIAELVRMGNFTARPL
                     FWTAVAFLLVCYLVGGWMVQQGYANGPAEIVVGSMTTTDPIVAVTFGLAVLGEGVLLG
                     GWDAVGMVISGAVAVAGVVVLSKYHPDAQVARPATQPATQPDPA"
     gene            complement(22794..23669)
                     /locus_tag="PKJ61_03560"
     CDS             complement(22794..23669)
                     /locus_tag="PKJ61_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HOC12936.1"
                     /translation="MNGVLLRDVPVWAVAAALPLVLLVAWVFGAIARWVLRGRARLGV
                     ATSMVISVLGTSVGLFLAGWISPQVLLWSPLTLALALGSSVLGIAGYAAVAAHFQHPQ
                     RATTAELLHAGESDRVEFKSTARVNLRTGAKDERMEQVVAKTICAFLNADGGTLLIGV
                     DDAGVPLGLAPDFGTLKSADADRFELWLRDLITSSMGQNAAAQVGVEFAELPDAAGVP
                     ALVCRVTATPSPRPVYLRAGKAASPEFWLRTGNSTRQLSVDEAADYVNHRWPMGVGAN
                     LAAQLRAAVRFSEAG"
     assembly_gap    23768..23867
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<23868..24952)
                     /locus_tag="PKJ61_03565"
     CDS             complement(<23868..24952)
                     /locus_tag="PKJ61_03565"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HOC12937.1"
                     /translation="MIRMLCRSLAGALVVLGVLSWAPGQAFADSDDDSVSNYQVIAKV
                     DASGKAAIELRLDFDFGSDSGHGPYLTFPLRQEIADDPDHWRMIDIEVAGANSPSGAN
                     AELETKTEDGNLLVRVGNEHQEFSGTQSYVIYLSMRGLIAPDHAQSGLDEFNWNAVGT
                     GWEIPIDNASATVRGPAYVTKSACFTGYGFTEACVAQQSGESATFSTMGIGEGTGMQV
                     VAGFPAGTFTGAEARLERRFTVSNMFPVTPVTGGVTAVLAALGLAAVFRRTRRSRRDQ
                     VYLGLTPGVVPAPNQEVSVGYASQDAPVAVRFNPPDGARPGELGTLLDATADNSDVTA
                     TIIDLAVRGHLQITQTGKKDWTFTRLRR"
     gene            25077..25982
                     /locus_tag="PKJ61_03570"
     CDS             25077..25982
                     /locus_tag="PKJ61_03570"
                     /inference="COORDINATES: protein motif:HMM:NF040269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC12938.1"
                     /translation="MTAQAVAAELLAVVVAMVDGQPSVLTLGEPPRLPAGPLLPSHRS
                     LQAATRAWVEEQTGRRLGYLEQLYTFADLDRDDGRGGRPISVSYLGLTNPGDELAIGK
                     WQPWYDLLPWEDRRPDVAGIDVTGPLLAWADTDLETSTERRQRVAINFGLDEHSWQPE
                     LVLQRYELLYEAGLVPESPSAWRRPDAELAAGVRMLGDHRRILATALARLRAKITYRP
                     VVFELLSPTFTLRQLQDCVEALAGRAVHTQNFRRLIDQQALVEETGELETSTGGRPAR
                     LYRFRRQVLAERHFSGTKLPTSRVR"
     gene            26101..27093
                     /gene="nadA"
                     /locus_tag="PKJ61_03575"
     CDS             26101..27093
                     /gene="nadA"
                     /locus_tag="PKJ61_03575"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF006879.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HOC12939.1"
                     /translation="MTANVTVETAADCYPRVANVVPAVEWATMADDVDAILRLKRGRN
                     AVILAHNYMTPEIFHGVADIVGDSLALAREATTVEADVIVLAGVHFMAETAKLLNPSK
                     TVLIPDLASGCSLAESITPADVRALRSAHPGVPVVTYVNTSAAVKAESDICCTSGNAV
                     RVIESLQVPKVIMIPDRFLAANVARQTGVEVIVHPGACEVHERFTPADIEQVRVDYPG
                     VTVLAHPECPPEVVDVADYAGSTAQMLNYVEVRRPAKVALITECSMSDNVAQQFPELE
                     FVRPCNLCPHMKRNTLANIRYSLETLTNEVIVPEDVADRARLAVERMLAVGTGK"
     gene            27090..28589
                     /locus_tag="PKJ61_03580"
     CDS             27090..28589
                     /locus_tag="PKJ61_03580"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF005701.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HOC12940.1"
                     /translation="MTHVVDAGCPVIIGAGLAGLVAAIELSPMPCIVLSAGAVSTGTS
                     TGWAQGGVAAAVGPDDDPTLHAQDTLAAGAGLCEPEAVQAITAAAPDAIRWLAEQGAR
                     FDHEADGSLRLGLEGAHSRRRIVHAQGDGTGAELLRTVVDSARELSSVSLWDHSPATD
                     VIVSGGRVVGVRVSTPDGPVEVLSPTVILATGGIGGLFSHTTNPLGSRGQGLALAHRA
                     GATLRDVEFVQFHPTALDVHLDPMPLVSEAVRGEGAILVNEGGEWVLANPLSARDVVS
                     RAEWAQLQAGHRVFLDAREHPGERFAALFPSIHATALAAGLDPARDLLPVRPAAHYHM
                     GGVLVELSGASSVSGLYAVGEVASTGLHGANRLASNSLLEAVVCGRWTAQHLRRTANL
                     TPTKAPLPDWRTWGDVTGSRPADPRVAAVRQIVSRHAGVLRDEDGLATALTALQPYLD
                     TDAGLVAELLVSAARGRTESRGAHTRTDHPGTVEPAEHTHTAALEGAAR"
     gene            28586..29443
                     /gene="nadC"
                     /locus_tag="PKJ61_03585"
     CDS             28586..29443
                     /gene="nadC"
                     /locus_tag="PKJ61_03585"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HOC12941.1"
                     /translation="MRALPRIVIKPLVRAALLEDLGRAGDLTTDAIIDAEATAEVALV
                     TREPGVVAGLGCAALAWELVDPRIVLTVERPDGTRVQPGDVIATASGPARGLLTGERV
                     ALNFLGHLSGVATGTATIADAIAHTAARVADTRKTIPGLRALQKYAVVAGGGANHRFG
                     LDDAVLIKDNHIAVAGGVRAAVTRVRAHVGHLVKVEVEVDSLAQLAELLEVGADAVLL
                     DNMTPELLREAVAMVDGRIVTEASGRITPETAVPIAEAGVDLISVGWLTHSARVLDIG
                     LDHVTGTAG"
     gene            30177..32741
                     /locus_tag="PKJ61_03590"
     CDS             30177..32741
                     /locus_tag="PKJ61_03590"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HOC12942.1"
                     /translation="MTADTVNETSLPAAPTDESPSTDSRQVGPMFPPTSPIALAPVTS
                     PAFSVDGFVREFLRELNTGQGVALSRSTINDQYLALARTVRHYLMARWLETLRHNRTT
                     KAKVVGYLSAEYLLGRQLGNALMASDLNDIADKAMAACGLDLATLRAQEVEPGLGNGG
                     LGRLAACFIDSLATMSVPCIGYGIRYEYGIFRQTFVDGRQVEQPDTWLSLGGPWEFPH
                     PEAAVQVDFGGSTETYDDHGIERTRWVPAWNVLGIPYNYMVPGYQNGRVNTLRLWSAQ
                     ATKAFDLHIFNSGDYAEAVRAQTFAENISKVLYPEDSTPQGKELRLQQQYFFVACSIR
                     NFIEEVLEQDFDLHNLSERIIFQLNDTHPVIAVPELMRILVDEKKFEWDEAWEVTRKC
                     FAYTCHTLLPEALEVWPVELMERLLPRHMEIIYRINEDFLAEVRAAYPDDELRARRMS
                     IVADYPTRSVRMAYLATVAGTKVNGVAALHSQLLADKVLPDFSGFWPEKFTNVTNGVT
                     PRRFLKMANPSLSSLITDAIGPTWVTNLERLQELEPYAEDEEFRSAFREAKEKNKARL
                     HALLRVRDGIDLPGGHLLDVMVKRLHEYKRQSLKLLHLVTLYDQLISGTVDPATITPR
                     TVVFGAKAAPGYRMAKETIYLINRVGATINSDPRVAGRLSVAFPANYNVTLAEKLIPA
                     ADLSEQISLAGMEASGTGNMKFALNGALTVGTDDGANVEIRQLVGDDNFFLFGMAEPE
                     VAALGAAGYQPTAYYEANPQLKSAIDLISSGAFSDGDRHAFEQVVSNLLYEDRFMALA
                     DYQSYIDAQAEVERRYADVDAWTKSAILNVARAGFFSSDRSMRDYLARIWGASPEM"
     gene            complement(32784..33887)
                     /locus_tag="PKJ61_03595"
     CDS             complement(32784..33887)
                     /locus_tag="PKJ61_03595"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HOC12943.1"
                     /translation="MSRIVVALLAGVLLCGCTSGVSTGSTGGVGGSTSGTEVSTSPDA
                     TGSAPASAARRVLATKLDVPWDFVRLPDESVLITLRDRGELVRLASSGELQTVAKVDG
                     VRPGGEGGLLGIALSPAFASDQLLYLYYTAAADNRVVRYRYDGGLADSRPIVTGIPKA
                     GNHNGGRLRFGPDGNLYIGTGDAGNTRNSQDRDSLGGKILRVHPDGSIPDDNPFGNAV
                     YSYGHRNVQGLGWDASGRLYASEFGQNAYDELNLIKAGANYGWPQTEGATNASGVTAP
                     LLVWTTDEASPSGIAVTPVGEIYLAALRGERVWHSRLTSNGLSEPDVFLRDLGRIRAV
                     EAVGDQLWVLTNNTARGNPGADDDQLVAVPLGT"
     gene            complement(33910..35232)
                     /locus_tag="PKJ61_03600"
     CDS             complement(33910..35232)
                     /locus_tag="PKJ61_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOC12944.1"
                     /translation="MTSAERQPPTRAERLDRLPFTSKHTKLLVGSGVGWALDAMDVGL
                     IAFVMAALRTEWQLDTTTLSWIGSIGFVGMALGATLGGLLADRIGRRQVFAITLLVYG
                     IATGASALATGVAALIALRFVVGLGLGAELPVASTLISEFAPRRIRGRMVVALEAFWA
                     LGWLAAALIGYLLIPNVANGWRWALAIGVIPTAYAAVVRWGLPESVRFLELKGRFTEA
                     EQAVVGFEQAAGVESVPSPDVVPHVPASELSIWSVVYRRRTAALWAVWFLINLSYYGA
                     FTWLPTLLVERGFPLVKSFGFTVIITLAQLPGYAVAAWLIEKWGRRSTLATFLLGSAI
                     SAAAFGLAGTDAAIIAAGSALSFFNLGAWGALYAIGPEIYSTGVRGTGTGAAAAFGRI
                     ASILAPLSVPLFLAGGGLVLVFGVFGVAFLLAAGAALLLPEAKNRPLA"
     gene            complement(35273..36451)
                     /locus_tag="PKJ61_03605"
     CDS             complement(35273..36451)
                     /locus_tag="PKJ61_03605"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOC12945.1"
                     /translation="MSGRAEGLVPVMGAGKRFAARPSGRLRRLIGRIGRRALLVTVVA
                     LVVGPLAYILLPIPSGAAPGGPTPVPLAAPVVVQVVQPSVTAVAALVGGIVETDDLTA
                     NPIGVVTGTTLADVDGLIGWPVTDHTPSTGFGYRSDPFTHRQRWHDGVDLGQPCGDPA
                     WASLDGTVTYTGWAGGYGNRIILKHADRSGHTFETAYSHLSQIDVTVGQAVKQAETIG
                     RIGSTGRSTACHMHFEVILDGAYVDPMRFLTGDQTKAALSRRVGSRMPQGMPSPSGTD
                     TSSPYASTRTPKPSSATPSTTTSTPAPISPVAAGASDTSAAGTDSSAPASNTPSSTGT
                     ETPAVTPSPTPTPTPTPSPESPSPETPSGDVPNEDSPVASSAEEGTSAQQSGATDSAG
                     "
     gene            complement(36597..37019)
                     /locus_tag="PKJ61_03610"
     CDS             complement(36597..37019)
                     /locus_tag="PKJ61_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family peroxiredoxin"
                     /protein_id="HOC12946.1"
                     /translation="MTVRSAQAHWEGSLIEGEGEVEFLNSNLGSVQVDWRSRSAETEG
                     RTSPEELIAAAHSACYSMALSHALAGNGTPPAAVDTRADVTFVAGQGITGIVLTVRAS
                     VPGLTAEEFAKFAEDAKTGCPVSAALTGTTITLDAALV"
     gene            37105..37827
                     /locus_tag="PKJ61_03615"
     CDS             37105..37827
                     /locus_tag="PKJ61_03615"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOC12947.1"
                     /translation="MSTPPPVAASPVDRALLVTGGVGIAVFTALILASAWVYDAVVDA
                     DGVSGLDRPVLDWALGVRTPAAEYWVTAFTNLGKTVPMVILGLILTSLIGWRWRRRTA
                     WVLMLVASAGSVTFTIVSKAMIARARPPLVDAVPPYEDSFSFPSGHTLNSTVVAGMLA
                     YLVVWLSRTLWVRLLAVLGAVLWAGPMGLSRVFLGHHWLTDVMFAWMFGLGWLALLIT
                     VHLVVLGVQRGRQDRISQRSAD"
     gene            37857..37929
                     /locus_tag="PKJ61_03620"
     tRNA            37857..37929
                     /locus_tag="PKJ61_03620"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:37890..37892,aa:Ala,seq:ggc)
BASE COUNT         6364 a        13146 c        12636 g         5685 t
ORIGIN      
        1 ccgccgaagc gcatggtcac gtgggacgcc tcgagcagca ccgtcttggc atccacgcga
       61 tggccgggag cagttgtctg ggtcatagtt cgaacccctt ctgctctggg ttgaagtggg
      121 cgtggcggag gttcagctct tcctcgatct cctccgcaag ctcctcgtcc tcgtcatgga
      181 actccagctg acgacgctcg tcggcgatga tgccctctgg acggaactgc atcatgaaaa
      241 ccatcagcag gccaaacagc agcaagcggt agtccgcgaa gaaccgcagc ttctccggca
      301 gcagcttcaa gatggcagcg ccgaccagca cacccatcac ggtgcccata ccgccgagca
      361 ccacggccgc cagcaggaat gccgactcca ggaagacgta ctggtcaggg gtgaccgaca
      421 cgtcgtggtg ggccttgacc gcacccgcga tgccggccag gaacgcaccg ccagcgaagg
      481 ccagcagctt cagggcgaag gtgttcacgc ccatcgcctc ggccgccagc tcgtcctcgc
      541 ggatcgccac ccaaccgcgc ccgatccggg agtagttcag gttcatgaac accacgatga
      601 tcagggcgat cacaatcagc atcagccagt agtagttggc gaaacggccg atcgggacgc
      661 ccagcaggac gtgcggatca ccgaagttga agccgaagaa ctccaggtcg ggaatgccgg
      721 agatgccgtt cgagccgttg gtgatgttcg ggccgtcgtt tccgtccagg ttgttcatgg
      781 tgatccggaa gatctccccg aacgccagag tgacgatcgc caggtagtcg ccggagaccc
      841 gcagtgttgg cgcgccgatg atcaggccaa ggatggacgc gaccgcgccg gcgatcagcg
      901 ccgtcaccag gaatggcggc ttgatgccgg cgtagtgcga gaacgccgag tccgaaagga
      961 tcgcggcggt gaaggcgccg gcgccgagga acgcgatgta gccgaggtcc agcagtccga
     1021 ccaggccaac cacgatgttc aggcccagcg ccgcggcagc gaagatcagc acctgggagg
     1081 caatcgacat gttcgcgtcc gagccgttct gggtgaacgg gaacgcgaac gccaccagga
     1141 aagcggagaa gaccaggacg cggttgttgt tggcggcagc caccccgagc cagccgaaga
     1201 ccccgaactg gcgcagcacc agcacgatgc tgaccaggaa ggcggcgaac gtgaggaacg
     1261 ccccggcgtc gttctggttg agcgcgaagg ctgcggcgaa cagcgccgaa cccatcagca
     1321 gggcgatccc gacgatctgc aaccacttgg ggctcttcac ccggccaagg gacggcgtcc
     1381 gtgaatccgg caggaagagc gtggccgcga tggcgaccac gccgccgatt agggccaacc
     1441 agccgccgat ctcgatgttg accaacccac tgagttcgat cgcgatcgca gcgagcgcga
     1501 ccagcaccgt ggccgtcgcc ccgatcgcgg ccgtcttggc ggtcgtattc cacgccacga
     1561 ccttggcgaa cctgcccagc cgcgacttgc ccaccagcgg cgcagccagg agtagcagga
     1621 gcagcaccgg cagcagcacg aaaaacagct gcagcgggga cggcgcgccg gcatagccga
     1681 cgtcgtcgag tgcaccgtga ccgtacgccc acggcaggaa gatggagacg atcatcagta
     1741 ccgcgcccac gaggccgatt gctcgtgccg ggtagcggta cctgatcttg gtctcgtcgc
     1801 tgatcacggt tctcatgcgc ggtccaccac cttggcgccg agcagaccct gtggccggaa
     1861 gaccagtacc aggatcagca ggatgaacgc ccagacgtct ttccaggcgg tggtgcccag
     1921 gtagttgctg gccagggcct caaccagacc tagcacgagg ccgccgacca ctgcgccgtt
     1981 gatgttgccg atgccgccca gcacggcggc ggtgaacgcc ttcagaccgg ccatgaagcc
     2041 catccggaaa tcgatgttgc cgtagcgcag cccgtgggcc acgccggcga cggcggccag
     2101 accggcgccg accgcgaacg cggcgacgat gacccggtcc acgttgatgc ccatcagctg
     2161 ggcggtgtca cgatcctgcg aggtggccac catcgcgcgg cccgtcttcg tccggttcac
     2221 gaagaaccag aggaacgcgg tgcagaccac cagcgcgccg acggtgaaga gcgccgacat
     2281 ctggatggtg actccgccga cgtgaatgat cgcaccggag atgcctggaa tggctggaaa
     2341 ggggacggcg ctcttcgcgt ccggcatgcc gggaatgcgg ccgaagaaca gtcgcaccac
     2401 ttcctgcagg aacaccgaga caccgatcgc cgagatcaac ggcgccagcc ggggcgcatt
     2461 gcgcagcggc cggtaggcga acctttcggt gagcagcgcc accgagatcg acacgatcac
     2521 gccaccgagg atcatcaacg gcaacagcac gaacaacgag gcggagacgc cccagttcgt
     2581 tggtgcgccg agcagcatcc aggtggcgaa tgagccgaag gcacccatca tgaagatctc
     2641 gccgtgggcg aagttgatca gttggacgat gccgtaaacc acggtgtagc cgaccgcgat
     2701 caacgcgtac agcgagccga gagagagtcc gttgaacaac tgttgcagga ataggtccac
     2761 tgccgctgta cccctttgct atcgacgcca cgaccccttg gcgctcgtcc gcggacgagc
     2821 gccaagggca actagctcac tggagctcgc cggtcttctc ggccttccaa gcgccgccgg
     2881 tcaccttgta gacggtcagc acccgggaca ccgagtcacc gaactcatcg aaaccgacct
     2941 tgccggtcgc accgtcgaac gagaccttgc cgatcgcgtc gatggtggcc tgtcgggccg
     3001 acttggcatc ggtggccgac gccaggctgg tcttcagcgc agcgatgatg gcgttggcag
     3061 cgtcatagcc gtaggcaccg taggcggaga agccgtcggg gaagccggct gccttgtagg
     3121 cctcgacgaa ggccttcgcc gaggccaggg tctcgaccgg ggcacccacc gaggtggcca
     3181 ggtcaccgtc ggtgccaccc agcttgatga agttcgcgtc gtagataccg tcgccgccca
     3241 tcagcgggac gttcaggccg gcggccttca tctgcttgga cagcgggccc gcctgcgggt
     3301 actcgccgcc gtagtacagg gcttccggac cggctgcctt gaccttggtg atcactgccg
     3361 agaagtcgct gtcgtcgggg ttgatggttt cggccgcgac gacggtgccg ccgccggcct
     3421 tgaacgcctc ggcgaaggcc tcagccaggc cctggccgta ggacttcttg tcgtggacgg
     3481 tggccacctt cttgatgccg gtgccgagca ggtactgcgc ggcgaacgga ccctgcaccg
     3541 cgtcagtggt gcagacccgg aagtagttgg cgttgggacg cttcgggttg gccaggtcgc
     3601 tgcccttggt gagcgccggg ttggtgttgg ccggcgagac ctggacgatc ttggccgcgt
     3661 cgagcaccgg gatcacgctc tgcgcgaccg aggagttcag cgtgccgacc acaccgacga
     3721 cggcatcgtc gctggccagc ttggtcgcag cgttcttgcc gacatcgggc ttggcctcat
     3781 cgtcctgggc gtccagttcg agcttccagc cagggatcac gttcttgtca ttggcttcgc
     3841 ggatcgccag ctcgaccgag ttgcggatgc cgaggcccat cgcggacaga ccacccgaca
     3901 acgggacgat gacgccgatc ttcgcgacct tggtggcgcc gccaccgcca ccggtgctgg
     3961 cttcagtgcg ggaaccgcaa ccagtgaatg ccagcgaggc cacaaccagg gccactccgg
     4021 cgagcttgaa tgactgagtc ttcaagtttc cctcctcaat gtgatgggca cccgtccttg
     4081 cgggttgggc aaggttatgg gctgcgcaca ctcaagcacc cgtgtgtggc ggcattgtta
     4141 cagaaccgtt gcgtcgttcg gcagcaccga atcgtcgtgt cttctactaa tcgagaagat
     4201 cgcggatcac cgcgtccgcc agcagtcggc cgcgcagggt cggacgcagg ttcccggcct
     4261 ccagccggac cagcccggac gccagcattc cgggcacccg accagcctca gaagaggtca
     4321 gcacgctcac cggaagacct tgtgaaagcc gtaattcgag cagcacccgc tcgaccctgc
     4381 gttgctcgtc ggtgagcagt tcacgagcct gcgcgggcga gctgccggcc gcgagtcggg
     4441 cgctgtagtc gcggggatgg cggacattcc accaacggac gccaccgacg tgcgaatgcg
     4501 cgccgggccc gatcccccac caatcgtcgc ccacccagta ggcgaggtta tgccgggagg
     4561 cgtggcccgg ctgggcccaa ttacttactt cgtaggccga aaatccctgc ccggtgagca
     4621 actcctcggc cagcaggtag caatcggcct gcaggtcctc atccggagcg ggcacctggc
     4681 cgcggccgat ctgggcggcc agccgggtgc ccggctcgac gatcagcgag taggcgctga
     4741 cgtggtcggg cgcgaaggag agtgcggcct ccagactcgt ccgccagtca tcgatggtct
     4801 cggtcgggct gccgtagatc aggtccacgc tgaccgaggc gaagcccgcg gcgcgcgccc
     4861 actcgaccac ctgcctcacc cggcccggcg tgtgccggcg ctccaacacg gccagcaccc
     4921 ctggccgggc cgactgcatg ccgaggctca accggttgaa gccggcagcc accagccggt
     4981 cgaggtactc ccggtcgacg ctctccggat tcgcctcggt ggtgatttcg gcgtccggcg
     5041 cgaccgcgaa ccgcgacctc gccgaggtca gcaggccggc cagtgccgct gccggcagca
     5101 tggtcggcgt gccgccgccg aagaagatgg tctgcagcgg cggcgggcaa ccgagcaccc
     5161 ggacggccag ctcgagttcg gcctcagccg cggccaggta ggcgtcctgg cctgcccccg
     5221 gcccggctcc gagctcggct gccgtgtagg tgttgaaatc gcagtagccg cagcgggtcg
     5281 cgcagaacgg cacatgcagg tacaggctca gccgacgctc cgccagcccg gccagcgccg
     5341 cggccggcag cgaaccgtcc gctggtgccg gcaggccgtc cggcggctgg gagggcacgg
     5401 tcagggcagg acgaacggca gctgctgtcc gtcgtgcacg cggcggcaca ggcaggccgc
     5461 ggtctcgtcc gattcggcgg cgggcagggc agcgatcacc cgcaccaaca gggtcttcag
     5521 ccggtcgatg ttgccggcga agacccgcat cacctcggcg tgcgtgaccg cctcccccgc
     5581 ttctacgccg gcgtcgtggt cggtcaccat gcacacggtc gtgtagcaca tggccagctc
     5641 acgggcgatc gcggcctcgg gcaccccggt catgcccacc accgtgccac cagcggcggc
     5701 atgccagcgc gactcggcgc gagtggagaa gcgcggcccg ttgatcacca ccagcgttcc
     5761 caccggcacc agcggcgtgg tctcgtcggc ggaggccagg acggccgccc gtccccgcgg
     5821 gcagtaaggg tcggcgaagg cggtgtgcac cactccgccg acctcgccgt agcaggtgtg
     5881 ctcgcggccc caggtgcggt cgatcacctg gtcgggcacc acgaaggcgc ccgggccgtg
     5941 ctcggcgacc agcgagccga ccgcagccgg cgcgatcacc tgacggacgc ccaggctgcg
     6001 caaggcccac aggttcgcgc ggtagttcac cttgtggggc gggtattcgt ggttgacgcc
     6061 gtggcgtggc aggaaggcga ccttgcgtcc ggcgacctca ccgacggtca gcggcgcact
     6121 gggcgttccg aagggggtgt ccacctcgac gcgctcggga ttgctgagga aggtgtagaa
     6181 gccgctgcct ccgatgatgc cgatctctgc gtccatgcgg gtgagcctaa tggccaccgc
     6241 cgacacccgc gattccgcac cccatgggaa cgaactcaga gccgcatcag ctcgtcaagg
     6301 gtcaggctcg ggttcgccgt cccggctcgc atcacttcga ccatgaaccg ctggcagtcg
     6361 tcgatcacct cgaccggcag gtggtccacc tggcgttcca actagcagcg cagcccaccc
     6421 gagccgtcat cgtccatcac ggtcaggtag cagagcaggc tgaacgcacc gttgcagacc
     6481 cagtcgaccg aagccactcc gccaagatcc atcagccggg tgccgagcga aaacctgtag
     6541 aacccagaac ctgcgcatga ctcccccaag taccgcgccg ttcgagccgt cccgggaacc
     6601 ggtcggctcc ttctactcca atctgccacc gcgtggcggg ttggcagccg aaccgcagca
     6661 attcgccgcc cgagtaattc gctccagtgg gggcctactt cttcttttcg gaagtctcct
     6721 gcgtggacag cgcggcgacg aaggcctcct gcggcacctc gacccggccg accattttca
     6781 tccgcttttt gccttccttc tgcttctcca gcagcttgcg cttgcgggtg atgtcaccgc
     6841 cgtagcactt ggcgagcacg tccttgcgca tggcacggac ggtttcgcgc gcgatcaccc
     6901 gggcgccgat ggccgcctgg atcggtacct cgaactgctg ccggggaatc agctccttca
     6961 gcttcttggc catggccacg ccgtagctgt aggcggcatc ggtgtgcacg atcgagctga
     7021 acgcgtccac cggttcgccg tgcagcagga tgtcgacctt caccagtttg gccacctgct
     7081 cgccgtcctc gtcgtagtcg agagaggcga agcccttcgt ccgcgacttc agggcgtcga
     7141 agaagtcgaa cacgatctcg gcgagcggca gccggtagcg gatctcgaca cggtcctcgc
     7201 tgaggtagtc catgccggtc tgctcaccgc ggcgcgcctg gcagagttcc atgatcgtgc
     7261 cgatgtactc ggccggtgcg aggatagtcg ccctgaccat gggctcgtgc acctcggcga
     7321 tcttgccgtc ggggtactcg gacgggttgg tcacctcatg ttcggtgccg tcctccatga
     7381 ccacgcgata gaccacgttc ggcgcggtgg agatcaggtc gaggttgaac tcgcgctcca
     7441 gccgttcgcg gacgatctcc atgtgcagca ggccgaggaa gccgacccgg aagccgaagc
     7501 cgagcgcgcc ggaggtctcc ggttcgtaga ccagcgcggc atcgttgagc tgcaacttgt
     7561 ccagcgcttc ccgcagctcg gggaagtcgg cgccgtcgat cgggtagagg cccgcgtaga
     7621 ccatcggatg cggcggacgg tagccgccga gatcctgctt ggccggacgg gaggccgcgg
     7681 tcacggtgtc gccgacccgc gactgccgca cctccttcac gccggtaatc aggtaaccga
     7741 cctcgccgac gccgagcgac tgcgccttca ccggctcggg cgagatgacc ccgacttcga
     7801 gcgtctcgtg ggtggccttg gtggacatca tcaggacgcg ttcgcggtgg ctgagctcgc
     7861 cgtccaccac gcgcacgtag gtgaccacac cgcggtaggt gtcatagacc gagtcgaaga
     7921 tcagcgcgcg cggcggcgca tccggatcgc cgacggggtg cggaatgtcg gccacgacgt
     7981 ggtcgagcag ttccttgaca ccctcgccgg tcttggcgct cacccgaaag atgtcgtcgg
     8041 ggtcgcagcc gaccaggtgc gcgatctcgg cggcgtactt ctccggctgc gcgctgggca
     8101 ggtcgatctt gttgagcacc ggcacgatgt gcaggtcggc ctcgagcgcg agatacaggt
     8161 tcgccagggt ctgcgcctcg attccctgag cggcgtcgat cagcaggatc gcgccctcac
     8221 acgctgccag cgaacgggag acctcgtagg tgaagtcgac gtgccccggg gtgtcgatca
     8281 tgttgaggat gtagtcagtg ccgtcgacct gccagggcat ccggacggcc tgggacttga
     8341 tcgtgatgcc gcgctcgcgc tcgatgtcca tgcggtccag gtactgggca cgcatcgagc
     8401 gggcgtccac caccccggtg agctgcagca tgcggtcggc aagggtggac ttgccgtggt
     8461 cgatgtgcgc gatgatgcag aagttgcgaa tcagcgcggg tggggtcttg cctggtgatg
     8521 gagtcggcat gcactttcct ggttgcggga cagccagcac acgataccga acccagccgg
     8581 atcgggcctc ttcgagctgc atccgggctc aggccgagcg agcccggcga gggcgggcag
     8641 gcccggagcg gtcaggcgat cgagctggtc ggtgggagac atggcccaag catcctggct
     8701 ccgaccagcc gaacagcggg ggtgatttga ggctggcttg gctgcctgat aaagttcgaa
     8761 atcgcgtccg tgcggcgcag cgtagtcaaa caccagcgaa agagctcatc ggcgtggcga
     8821 acatcaagtc tcagaagaag cggatcctga ccaacgagaa ggccaggctg cggaacaagg
     8881 ccgtgaagtc cggtctgcgt accaacgtcc gcaagttcaa cgaggccgtc gctgccggcg
     8941 atagcgagaa ggcgcaagag ctggcgaaga ccgcgacccg ttcgctggac aaggccgcga
     9001 gcaagggcgt catccacaag aaccaggcag cgaaccgcaa gtcggccatc gcagccaagg
     9061 ccgccgcact ctgatcttcc tcacaaaacg ccgctcggtt gcccgagcgg cgttttgttg
     9121 tgcgctcgga cgctaacgcc gctcgctgcg acggcactca atcacgccga gcaccaattg
     9181 ctcaagcgcg aacccgggat cgctggcagc acccttgatc tgggcgtcgg cgatcgctac
     9241 ctgccggatg gcagcggcga ccgcggtcgg cttccagtcg cgggacagcc gggagaggtc
     9301 cttcaacttc cacggcggca cgcccactac ccgggccagt tcggcgtcgc gaagccgggc
     9361 gtcccggccg tcgagatact tgccgaggct gcgcaggttg ttcgaaagcg cgctcgtgat
     9421 caggaccggc gcgacaccgg tcgaaagggc ccagcgcaac ttgccgagcg caccgtcgac
     9481 gcgtccgctg agtacgtcgt cggcaaccgc gaaactggtc acctccgccc ggccgccgaa
     9541 gtagcgacgc acggcgacct cggtgagcag gcggtcgggc gaatcgtcca gcagttggcg
     9601 aaccgcacca gccagtgctc gagtgtcgct gccgaccgcc tcgatgagcg cgcccgcggt
     9661 tcttgcgtcc acccgtccgc cgcaccggcg aacttcgccc gccacgaact ggggcagatc
     9721 ccagggcttg atggcggcga agtcctgcgc cgggacgcgc agcttcttca gccggtcgag
     9781 cagcgccttg cctttgactc ccccgccgtg caccagcacc agtgccaggt cgtccccagg
     9841 gtcggtggcg agtgcgacca catggtcggc caactcggcg ggcaactccg ccaggttact
     9901 gaccaccacg atcgaggcgg acgcgaacag cgaacccccg gcggcttcgg ccaaagcgcc
     9961 cgcatccagc tctccggcct gcagccggac gacggccgcg tccgggcgct ccgcggtagc
    10021 ccgccccacg aaatcggcta cggcacgctc ggccagcagt gactccgggc cggtgaccag
    10081 tagagcggtt gcgaaggcgg tttcgggcac gggctaagca tgccaggctg caccgacagc
    10141 tccggccgag cgctggatca gctgaacggc cggcccgctg cctgggcgag ccagcccggc
    10201 acctcagcgg gatgatcgag ccgcgtgccg aacggcaccg tcaccgcggt gacgccgtcg
    10261 gcatccatgc acaccaggtc cagccggagc gaggacatgc tggtcaccga gatgatcgcc
    10321 gcgggcgcgt tggggtagcc gagggcaccg accaggatcc gcaggtcgaa ctgatgctcc
    10381 cgctcgatgc actcgacgat cccggctacg tccagcagcc aggattcgga gcgggccgaa
    10441 gcgtaggcgt ccagcgcgac cgggatcgcg caggcccggc acagatcggt ggccggggcg
    10501 aggaccgtga ccgcttcgag atcgacctcg acgaccgtga cctcggccgg gtcgacggca
    10561 tcgagatggt gctgcagcag gtggttgatc tccggacgcg cgatcgggca gaccgcgtcg
    10621 ccacaacggc cgtcgaggcg cagcacgcca aggccaggga caccggcccg gcaggtgatg
    10681 cggaagccgc cttcgggcag ccgcggatcc acgtcagcca ccaggtaggc cgagccgttg
    10741 ccgtccacca cgatgccgag attgccttgg ctgattacgc gacatcgtgt gcgcagattg
    10801 aagatcaagg gtggcatcga cggggccgtg gcccaggtgg gcgcatgctc gcgcaccgac
    10861 tgccgccaca acgcagatct cgaatgcacc gcggacgccg tccgcgtggg tgcgggcgcc
    10921 gacggcatgg ccgccaactg cctcacctac gagccggcct gacagttgcc tgacgattgg
    10981 ggcggtcgac gcgagtcggc cgccccaatc gcgttcagcc aggcttggtc gtcacccgct
    11041 gcgctgggtg gtgaccgtca actccccatc gcgccgggag accgcgatcg caccgctgcg
    11101 gtcggtgcgg aagatctgcg ccccagttcc ggcgacggag tccacggtgc gcgcggccgg
    11161 gtgaccgtag tcgttttcgg caccaacgct gatcagcgca acgctggcgt ggaccgcggc
    11221 cagatacgcc gcgtcctggt gagcagaccc atgatgcggt accagcatca cctcggcact
    11281 caggtcggcg acgcgggtga ccgcgttgcg ctgcccagcc tcctcaacgt caccggagag
    11341 gagcaatctg agctcaggag tcgtcactcg cagcaccagc gacgagtcgt tctgttcagc
    11401 cgagtcctcg ccggcgactt cgctgccgct caccagtgga cgaacggcca gcacttcgac
    11461 tgtggttgcg ccggcggcca ccaccatgcc gggcacggca acctgttcgg ggttgccggc
    11521 cagcgccgcc ctgactccat gccagttggc ggcaggctcg gtgatgccgg agtacagcag
    11581 ccgtccgacc gagcgtcccg aggccacccc ggccactccg ccggcgtggt cggaatggag
    11641 gtgggtgagg atcagccacg ggacatcgac gatgcccaat tggtcgaggc acttgtcgac
    11701 cagccctggt tcaggcccgg tatcgaccac gatcgcctgc ccttcgccaa tgctgatcac
    11761 ggtggcgtcc ccttgaccga catcgcagct gaccaccgcc cattcggttg gtggccaacc
    11821 cggagtggtg accggacgca gcagcactag cacctgcgcc gccactgcga acacgagcag
    11881 ccacggacgc cgcaacagtc gcggcatcag gaccagggtt ccggcgcaga gcaccaccat
    11941 caccgcagtg gccaggggtg tcgcgggcca ggccagagtc gcgcccggca gcgcgctgcc
    12001 ggcttccgcg atccagcaga ggctctgggc gaaaccgccg gcggcccagc cgagcagtgc
    12061 cgccaccggc gggctcgcta cggacacccc ggccgcgagg aagccgagca ccgtgccggg
    12121 cccgacgagt ggcccagcca ccaggttcgc aaccagaccc accaggctca cttgtcccga
    12181 gatcgctgac acgatcggct gggtggccag ttgcgcagcg gccgggacag ccaatgcctc
    12241 ggccagccac gcaggcaccc atgcctgcag cactcccgcc cacggccgcg cccagagcac
    12301 gatgcccgcg ctggcgcaca ccgacagggc aaacccgacc gagcgggaca gccacggatc
    12361 gaccagcaac agtccgatca cggcccagga caagtagcgg aggccctgcc gcgggccacc
    12421 ccaccccagc gcggccagac ccaccacgcc catcgccgcc gcacgcagga cgctcgcttc
    12481 agcccggcac aggaggacga aggccgccac cccgacgacg gccaacagcc gccgccacca
    12541 gcccagcact ccgacccgcg ccgcggccca ggtgagcgca gccaggagga gggtgagatt
    12601 ggcccccgag accgccgtga gatgggttag cccggtggca cgaaagcgat cctgaagggc
    12661 ttcggtcatg ccgctggtat cgcctacgac gagagccggc acgagcgccc tgggatcggg
    12721 cgggagccct gccaccgacg cccgcaatcc ggcccgaacc gaagtcacgg cctgatcgac
    12781 cgcacccggc tcgaccacga cggtcggaga tgcccgggct cgtgcccagg ccccgaccga
    12841 ttcgcctggt tcggcgggac tcagccgaac cagggcccgc actgtcgagc cggccggcag
    12901 gttggcccac gactcgagtt gcgtaccgcc ggccgagatg cggacggagg cgccggtgat
    12961 ccaagcgtca ccgcgacctt cggcatccac cagagcagca gtcgcgatcc acagcggacc
    13021 accgactccg ccgtccgtga tctggccgcc ggagacccgc gcggtgatag tgaccaccgc
    13081 gttgtcggcc gcccaggtgg ggatgggacc cacgctcgcc agccacacgc gtgcccacgc
    13141 cgttcccacc accaccagca tggtcacggc gatggcgatg cgaagccagt gccgcgaccg
    13201 ccaggccagc agaccgagtg ccagagccag cagcccgcac ccgacggccc accagttcac
    13261 ccccgaggtc gccgcccacg cccctgccca cgacgccatc gcgagaaacg ggcctcgcca
    13321 gtcgatcggc gcggcgtcca cggaaggcgc gactggcgtg ggtccgcgac tcatgggcag
    13381 gcccgagggt ggctgtggga cggctgggtg gtctcgacaa gcccgaccag ccgagaaccg
    13441 ggttggcgaa ccagccgaat cccagcttgg ccgaccagcc gagaccctgg cgggcgggcc
    13501 aacgtcaact cacacgcgca catggggttc gatctccgcg tagatcttgg ggccgattcc
    13561 gtccacctct tgaagctcgc ctacggatga gaacctgccg tgggcgtccc gccaatccag
    13621 gatcttcttg gccgtcaccg ggccgacccc aggcaacgtg tccaactgcg cgagggtggc
    13681 cgtgttcagc gagatcgtgc cagaacccga cgagccagcc gagcccgagc ttccccctgc
    13741 tccgcgcacc tcgccgccgg ggtcggcgtc ggtgccgatc accaactggt caccgtcagc
    13801 caccaacgcc gcaaggttga gttcgcccgg gtcggccgaa gcggacaatc cgcccgcagc
    13861 ttcgatggcg tccgcgaccc ggctgcccgt cggcaagtcc accagacctg gcttgcggac
    13921 cgccccgagc acgtgcacca gcagtctcgg ctcgggtgtg ggcgtcggcg tcggactcgc
    13981 caccaccgac ggcaccgcgg cgaccgccac cggggtggag cgggcttgca gcatggtgta
    14041 agcggtccag gcacagccca ccagcaggat gaccaccaca gcggccaggt gctcgcgccc
    14101 gaagtcgagc aatcgagtgc gcagctgggt gatgccgttg tggcccggcg ggtttgcggc
    14161 tcggcgcggc ggcggtgggt cggcagcccc ctggttcgaa ccggttgcgg gccggcccaa
    14221 accgggcgac ggcaggtgga agctgcctgg atcctgcggt gaggcgcgtg gcggaccggc
    14281 cgtggaagta tcgaccggcg cccacccttc cgtgccggac ggctgcaggc gacgcggctc
    14341 gacctccgcc agcagcgctt ccagccgcgc gcgcaccacc tcgctcatca tgggctctgg
    14401 cgcaaccttc acacctgcac tatcgacagc ttccaccgaa tccggtcagg cgaatctgcc
    14461 ggctgtggaa aacctccccc ggaatctcag ctatccacag cctgacttcg atcggagcca
    14521 tgcgccgggt tacccttggt gcgctcaacc ccgatcgcat cacaggaaat tcaaatggct
    14581 atagatatcg acctcaatga gaagaagacc gaaggcggcg gctgcggctg cggttgcggc
    14641 acccgggccg gcgtcccgga actacaggcc gccgcgttgc cgaaggtgat ccgccacggc
    14701 gcaatcatcg gcgggctgac cagcatgaag tccggcgagg cgctcgtgct cgtggtaagc
    14761 cacgacccgc tgccgctgct cagccagctc aaccgagtcg ctccggacgc cttcgacctg
    14821 gagtacctcg agcgcggccc cgaggcctac cggctgcagt tcaccaagcg ctgaggcgct
    14881 ccgcccaggc tcagggaacc tcgacaggtt cgaccaaacg cgacacgccc gccgacccag
    14941 gggtcggcgg gcgttcgctt acgtcacacc cttagcccgg gacgatgctg accagcttcg
    15001 gggcgcgcac gatcacctgg cgcacctcgc gtccggccag cgcgcgctgc actccatcgt
    15061 ccgccagcgc caccgcggtc aggtcgtcgg cggagatgcc gggcgaaacg tccagcttgg
    15121 cccgcacctt gccctgaacc tgcaccacac acgtgaccga ctcggtgacc agcagcgcgg
    15181 gatcggccac cggccagttc gcgttggcca cggacggtgc gccgccaagc agttcccaca
    15241 tctcctcggc gacgtacggc gcaacggtgc tcagagtgac cgccaccgtc tccacggcct
    15301 cccgaactgc cgcgtccgca ccaccgacac ccgagtcgat cgccttgcgg gtcgcgttca
    15361 ccaactccat gattcgcgcg accgccacgt tgaaccggtg gctctcgatc gattcggtga
    15421 tctcggcgat cgccttgtgg gtcacccgcc gcatcgccag gtcaccggcg gcgaagcccg
    15481 caccgggcgc gctggtcacg tccgcggcca gccggtaggc acgctgcagg aacttcaggg
    15541 atgaacccgg cgacatgtcc gcccagtcga tgtcgtcctc gggcggaccg gcgaacacca
    15601 cagtggtgcg gatcgcatcc accccgaagc gatcgatctg ctcaccgaga tcgacgccgt
    15661 tgcctagcga cttgctcatc gccttgccct ggttgatcac ctgaccctgg ttcatcagcc
    15721 ggctgaacgg ctcgctgaag tcgaccaggc cgagatccgc gagcaccttg gtgaagaacc
    15781 gcgagtacag caggtgcagg atggcgtgtt ccacaccacc gacgtactgc gcgaccggca
    15841 tccacttggc cacttcggcc acgtcgaacg gcccttcggt gtagttcggc gagcagtagc
    15901 ggaagtagta ccaggacgag tcgacgaacg tgtccatcgt gtcggtgtca cgcaaggccc
    15961 cgccaccgca cttcgggcag gtcgtgttca cccagtcggt cgcggacgcc agcgggctgg
    16021 tgcccttcgg ctgcagggcg gcgccccgca ggtcgggcag cgtcaccggc aactggtcgt
    16081 cggggacggg cacctcgccg cactcggggc agtgcacgat cgggatcggg cagccccaga
    16141 agcgctgccg actgagcagc cagtcgcgca gtcggaaggt gaccgcggct tcgccggtgc
    16201 cagcctcggt cagcacctcg ttggtcttgg cgaccgcctc cttcacgctg gtcataccgg
    16261 tcagcgggcc ggcgttcacg taggtgccgt cgcccttggt ggcgatgcca gactcggcag
    16321 ggtgcggatc gccactgtcc agcacgatcc gaaccggcag gtcgaatgcg cgggcgaagt
    16381 cgaggtcacg ctggtcgtga ccgggcatgc ccatcaccgc gccggtgccg tactcggcca
    16441 gcacgtagtc ggccgcgtag accggcagcc gctccccgtt gatcgggttg atcgcgtgga
    16501 cgccgaggaa aaccccggtc ttcggacgct cggacgactg ccgctcgatc tcagtggccc
    16561 gcttggtctg ctccagatag gcctcgaact cggcccgggc cgtgggcgcg cagatctcgt
    16621 ccgcgatcgc agcctcgggc gcgaccacga agaaggtggc gccgtagagg gtgtccggac
    16681 gcgtggtgaa gacccggacc ggcgcctcgc ggccctcaat ccgaaagtcg acccaggcgc
    16741 cctcggaacg gccgatccag ttacgctgca tcgcaagcac gcggtcgggc cacttgcctt
    16801 cgatctgctc catgtcgtcc agcagtcgct gggcgtaatc ggtgatcttg aagtaccact
    16861 gggtgagctt gcgcttggtc accaccgcgc tgcagcgctc gcacagcccc tgcacgacct
    16921 gctcgttggc cagcaccgtc tggtcgttgg ggcaccagtt gacgtaggag tccttgcggt
    16981 aggccaggcc gcgctcgaag aagcgcagga acagccactg ggtccagcgg tagtactccg
    17041 ggtcggaggt gtgcagccgc cgcgaccagt ccaggctgag cccgtaccgc ttgaaggagc
    17101 gcgcctgggt gtcgatgttg gcgtaggtcc aatcggccgg gtgcgcgtcg cgggcgatcg
    17161 cggcattctc ggccggcagg ccgaaggagt cccagccgat cgggtgcatt acgtcgtaac
    17221 cggccaggcg ccagtagcgg gacacgatgt cacccatgac gaatgcttcc gcgtgaccca
    17281 tgtggaggtc gccggacggg tacgggaaca tgtcgagcat gtagcgacgt tccttcgtgc
    17341 catcgtcggc tgggctgaac gtaccgtcgg cttcccagaa ccgctgccac ttctcctgcg
    17401 ctgcggccgc gtcgtagccg atccgttcct cggtcacttc gtctccttcg tgtggtgtgc
    17461 gcacccggac aagaaaaaac ccccgcgggc cgtcaggctc gccaggggcc gccgcggctg
    17521 ccgaaagcag tcgcggctag aaaagcaggg tgtacgtctg cacgatcgcc aatcctaacg
    17581 gatctggcgg acgctgttcg cccgcccagt cccagcgtca ccggagcggc ttcgccaggc
    17641 tcagccagca ttcgcggggg caggttccag acgttgtcgc agacggcggc agaaccgaga
    17701 gcccactgac ctaattcacc gcacaccttg aacgggtcac catccgagca ctttaggaca
    17761 gcctcacctt gcttgcgact agggcaaatg ccgacaacac tggagttgtg accatcacca
    17821 ttccggcact cgaggcgagc gagcagtccc tgtccgcccg actcaactgg ctgcgtgcgg
    17881 gcgtgctggg tgccaacgac gggatcgtat ccaccgccgg cctggttttc ggggtcgccg
    17941 gcgccactgc tgactcactc gccctactca tcgccggact ggccggactg gtggctggtg
    18001 cgctgtcgat ggccggcggc gagtacgtct cggtgagcag tcagaaggac accgagaccg
    18061 cagccttggc cgctcaatca cagcggctca gcgacgatcc cgaaggcgaa ctccagcgac
    18121 tcgccgatca ctacgccggc agcggactgt cctccggcct ggcccggcag gtggccgagg
    18181 agctcaccag ccacgcggca ctcgacgccc acgcccgggt cttcctgcac ctcgacgcga
    18241 acgagcgcgt ctctccatgg tcagcagccc gcgcgtccat gatcgcgttc acgatcggag
    18301 cgctgatccc cctgctggcc atcgtgttga ctccgcaaac gctgcggcta cccctaaccg
    18361 tggtggcggt gctcgcggca ctcaccctca ccggcgtcgt gagcgcacga ctcggcggcg
    18421 cgcgtgtgtg gcgagcgata gtgcgcaatg tcttggtcgg cagcctggcc atgggactca
    18481 cctacctggt cggctctctg gtagggcagc ttctctgacc cggtcggcca gctcgtccga
    18541 ccactccgcc agccacgcca gcaccggacg gcgaagctcc ggccaggcgc ccggcggcac
    18601 catctcgtga ttcagcttgt cgatgaccgc cgccaacggc aggccgggat cgattcgatc
    18661 gaggcgttcc aggcagacca tctgcagggc accgtcaccg gtctgccagg cagccatggc
    18721 caacagcccc aagggcaccg cctggtaacc gagcaggcaa tgccgcacca cacccgtcca
    18781 cagctccagt tgctcggacg cccgctcgcg gctcagggcc cgcagcgcga tcagcatgcc
    18841 ggccggttcg tccaccagca gcgccagccg tgcctgcgcg aacagatcgg gttccggctc
    18901 gcgcaacagg cggcgcagca agcgggcacg agccgaggcc gaggcacggt cgagcgcagc
    18961 cgctgcccgc tcgaacatcc cgagcagatg gtcgatctcc gcgtcgggtg gaccggcgat
    19021 gccctcggcc agcgccgccc gcgacgcccg agccggcagc ccaagcaccg cggcctgcgc
    19081 cgccgcggcc gtcacggtgc cgttgagcgc gccgcttggc ccgtccggat ggtctgcgcg
    19141 ccagaccgta tcggagacct gcaggagccg gccgacccga gtggccccga ccaggccgga
    19201 gcacgcgccg agcacctccc aggcgcacga gtcgtcggcg gagtaggcga agaaccagcc
    19261 aaccgcgcgt gggaatcgat cgaacaatcg gccgatcagg tcatccagcg cccctggctc
    19321 ggccacggcg gcaagatcca ctctcgcggt gacctccacc tggccggact cgatcaccac
    19381 gaccaccagt gactcggctg gatggaagcc gatcaggaac ggcaccaggc cgaccaggtc
    19441 gtcgatgttg cgtacggcca accgggccgg cggttccgag acgttcacac cagagcattg
    19501 tcggcaggtc gggctgattt ccgtcaccca tccacaggcg atgccggccg atcgacgata
    19561 tccacaggcg agcggtcgtt acctggtgaa gtccggcggg tagcgtagat gccgatgaca
    19621 tccgaaccca accgcgatcc cgcgtcccag cagccgcccg cgtccgcgcc gcacccgtcc
    19681 gcgccaccgg cgggctgggc acccacaccg ctctccggtg tcacgcggcc cgcagtcccg
    19741 cgaccagccg ccggcggcgg accgtccgct ccccttggcg ggtcgaacct gccgtccacc
    19801 aggatcgacg agttcgccgc accacgttcc tggtggccac tggtcgtcgg cgtagtcgcg
    19861 gtcctcgcgg ccgccctgat ctgggccagc accaccttgc ggccagcgct gccctcggcg
    19921 accaaaccgt ccccgactcc cgcccccaca gcgaccgcgc ccggacgccc cttcgtcagc
    19981 ccgaacgagc gatattcggg tcgctgggag atcctgcagt ccgagtggac cgacagcggg
    20041 gtggaggtgc agattcgcat ctcggtggac aagggcccgc tcagctactc cttcgtggct
    20101 ttcgagaaca acagcgtcac cgccagcgat ccgctgcccg ccgcccattc gccgcgcttc
    20161 tccggctacc cgatcgaaac cggcgacacc gagaccggct ggttgctctt cccgctggag
    20221 cgcgggcagg ccaccgtgat ccttgccaat gccgcgggca gccagatgtc ggccctgccc
    20281 atccccggtt gagccgctgc cagccgccaa gccgggctaa gcccggatcc accgatggcc
    20341 gtcgtcgcga gcggcaccag acgggtggcc tggctggccg aaccacgcga tggcataccg
    20401 ggcgcatgtt gagcgtggtc agcgacgcca gggtgcccgg gggtcgcgca gcagacggaa
    20461 atcggtggat ccgggctaag gtgtgcgggt gacggcgcca ctatcgatcg gcatcttcac
    20521 cgacgacttc tatcccgaat cgggtggtgt ctcgcggtcg atccaactgc agttgcagca
    20581 gttgagccag gccggacacg gggtcactct gttcgccccc aacttccagt tcactgcgcc
    20641 gtccgagtgc gcctgggagg gcctgccgta ctggcggctg cccggcacgc cgagctatct
    20701 atgctcgctg cagaccggac gtcggctggc tcgccggatc gccgcggacc accggctgga
    20761 cgtggtgcat tcgcagaacg agcgcggctc ggtgttcctg gccgcccaag tggcccgggc
    20821 cgcaaacgtg ccgcaggtgc acacctttca ttccaactac gtgggtaccc accgcaccac
    20881 gccgctggtc tctggcctga actcgttcac ctatctggac tggtcgggac gctggctgcg
    20941 ccgcaccgcc ggtcgcggta acgacgtcac gatcagatcc tcgagcgatc cggacgccgg
    21001 cagcgactcg cggttcgcca accgcgactg gcgaagcctc gctcgactcg cgtccgccgt
    21061 ggacgctttc acctcaccgg ccgcctacct tgtcgaagcg gtggtggacg cctcaggtgg
    21121 cgagttggac ggacgcggtc acgtcgtagc cagcggcgtg gcggacgcct tcgtccacgc
    21181 cgagcgcaag ctgcccagag gcggacgcgt ccgcttcctt agttgtggcc gtctcggccc
    21241 ggagaagcgg gtggacgtga tcctcgatgc gttcgaacgg ctcggccgcg acgacgccga
    21301 gctgcatatc gtcggcaccg gatcagccga gggtgccctg cgccagcagg cctcgcggat
    21361 ccggcacggg accgtccgct tccttgggca cttcgacgat gccgcgctga tcgcccagga
    21421 gatcgcggac gcggacgtgt tcgtgctcgc gtcctaccga ttcgacaccc agggcatggt
    21481 gctggccgag gccgccgccg cgggcacgcc catcctctac tgcgacgatc ggctgagcgt
    21541 tggggtgtca ggcgagaacg cgctgctcac cggcccgagc gcggcggaac tggccgctgg
    21601 catggccgaa ctggcggacg caccggagcg gctggtcgcc atgtcgaagg cgtcgaagag
    21661 actcggcgcg acgctcacgg ccgaggccat ggccgagcgc tacctcgcgg tctaccgcca
    21721 ggtgatctcg ggctgagctg ccccgagagc tgcgggccgg gctcaggctg gatcgggctg
    21781 agtcgccggc tgcgtggccg ggcgggccac ctgcgcgtcc gggtggtact tcgagagcac
    21841 cacgacgccg gcgaccgcca ctgcgccgct gatcaccatg ccgaccgcgt cccagccgcc
    21901 cagcaggacg ccctcgccga gcactgccaa gccgaaggtg acggcaacga tcgggtcggt
    21961 ggtggtcatc gaaccaacca cgatctcggc cggaccgttg gcgtagccct gctgcaccat
    22021 ccagccgcca accaggtagc agaccagcag gaacgccacg gcggtccaga acagcggacg
    22081 cgcggtgaag ttgcccatcc gcaccagttc ggcgatggtc ttgatcagcg ccgaggagag
    22141 cccgtagaag aacgagccgc ccgaggccca catcaggcag cggcgcgacg gcgagccgaa
    22201 gcgccccagc cagcccagaa cgccgccggc tgcgaacacg atcaggcagg acacgatgat
    22261 ccgtgccggt ggcagttcag tatccggcgc tgccgtgctg gccgagaaca gggtgaacac
    22321 gacgatcccg aggatggtga gtgcgaccgc cccccagatc accttcgtcg gccggaagtg
    22381 gtgaatcttc gccgccagca gcaccgccca gggcaccgcg aggatgccga ccggctggac
    22441 gaccgtgact ggcgcgagca tcagtcccac gatgtgcagg ctggcgccaa ggccgacggt
    22501 aagcaggccg agcagccacc gccggttcgt cagcagcgcg agcacctggc gcaggcccat
    22561 cgaccggttc tcggactcct tgtccaccgt ccggctgacc gcgaggtgct ggatggtgga
    22621 gccgccggcg aacaggaagg acgcaaacac cacgatcaga atggccagcg gaatgttctg
    22681 ctcgaacaac caggcgggcg cggccagcgg aattgacgcg gtcatggggt ccttgtctgc
    22741 tcaaaggcgg ccagcttatc ccagccgtcc cgacccagtt ggctgcccac cgctcaacca
    22801 gcttcggaga atcggacggc ggcccgcagc tgggcggcga gattggcgcc gacccccatg
    22861 ggccagcggt ggttcacgta gtcagccgct tcgtccaccg acagctgccg ggtggagttg
    22921 ccggtacgca gccagaactc cggtgacgcg gctttgcccg cacgcaggta caccggccgc
    22981 ggggacgggg tcgccgtcac ccggcacacc agcgcgggaa cgccggcagc gtcggggagt
    23041 tcggcgaact cgacgccgac ctgggctgcg gcgttctggc ccatcgacga ggtgatcagg
    23101 tcgcgcaacc agagctcgaa ccggtcggcg tccgcgcttt tcagggtgcc gaagtcgggc
    23161 gccaggccga gcggcacacc cgcatcgtcc acgccgatca gcagcgtgcc gccgtccgcg
    23221 ttcaggaagg cgcagatcgt cttggcaacc acctgctcca tgcgctcgtc cttggctccg
    23281 gtgcgcaggt tcacccgggc cgtcgacttg aactcgaccc gatccgactc acctgcgtgc
    23341 agcagctcgg ctgtggtggc ccgctgcggg tgctggaagt gggcggccac cgcggcgtag
    23401 ccggcgatgc cgagcacgga gctgccgagc gccagcgcta gcgtcagcgg gctccacagc
    23461 aacacctgag ggctgatcca gcctgcgagg aaaagcccga ccgaggtgcc gagcaccgag
    23521 atgaccatcg acgtggcgac gcccagcctc gcacgtccgc gcaataccca gcgggcgatc
    23581 gcgccgaaca cccaggccac cagcagcacc agcggcagcg ccgcggccac cgcccacacc
    23641 ggcacgtccc gaagcagcac tccattcatg atggggcaac cgtagcgtgc gggcgccggt
    23701 cgggtttcgg cgggtcagct caccagcccc caccgccgcc accaccgaaa ccgccgccgc
    23761 cggagaannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncgc cgcagccggg
    23881 tgaaggtcca gtccttcttg ccggtctggg tgatctggag gtgcccgcgc accgcgaggt
    23941 cgatgatcgt tgcggtcacg tcggagttgt ccgcggtcgc gtccagcagc gtgccgagtt
    24001 cacccggacg cgcgccgtcc ggcgggttga agcggaccgc gaccggtgcg tcctggctcg
    24061 cgtagccgac gctgacctcc tgattcggcg ccggcacgac cccgggggtg aggccgaggt
    24121 agacctggtc acgccggctg cgccgcgtcc ggcggaagac ggcagcgaga ccgagcgcgg
    24181 caagcaccgc cgtcacacca ccggtcaccg gagtcaccgg gaacatgttc gaaacggtga
    24241 accgacgctc cagccgggcc tcagccccag tgaacgtgcc cgcggggaag cccgcgacca
    24301 cctgcatgcc ggtgccctcg ccgataccca tggtcgagaa ggtggcgctc tcgccggatt
    24361 gctgcgcaac gcaggcctcg gtgaagccat agccggtgaa gcaggcgctc ttggtgacgt
    24421 aggccggacc acgaacggtc gccgacgcgt tgtcgatcgg aatctcccaa cccgtgccga
    24481 cggcgttcca gttgaactcg tccagcccgg attgcgcgtg gtcgggtgcg atcagcccgc
    24541 gcatggacag gtagatcaca taggactgag tgccgctgaa ctcctggtgc tcgttgccga
    24601 cccgcaccag caggttgccg tcctcggtct tggtctcaag ttcggcgttc gcgccgctcg
    24661 ggctgttcgc cccggccacc tcgatgtcga tcatccgcca gtgatcgggg tcgtcggcga
    24721 tctcttggcg aagcgggaag gtcaagtagg ggccgtggcc gctgtcactg ccgaagtcga
    24781 agtccagccg caactcgatg gcggctttgc ccgaggcgtc gaccttcgcg atcacctggt
    24841 agttcgagac cgagtcatcg tcggagtcgg cgaaggcctg ccccggtgcc cacgacagca
    24901 caccgagcac gaccagagca ccagccagcg acctgcacag catccggatc atgcgttcga
    24961 gcctaacaag agcatctgac ggccgctgcc cgtggctatc ctgactagac cctggtccgg
    25021 ccgcaggtcg gatcgtgggt gggagcgttg gagttgggcc gatcgattgg ggtgcgatga
    25081 ctgcgcaggc ggtggctgcg gagcttctgg ccgtcgttgt ggccatggtc gatggacagc
    25141 cgtccgtcct caccctcggc gagccaccgc ggctaccggc gggcccgctg ctgccgagcc
    25201 atcgctcgtt gcaggcggcc accagggcgt gggtcgagga gcagaccgga cgccggcttg
    25261 gctacctcga acagctgtac accttcgccg atctggaccg cgacgacggt cggggcggac
    25321 ggccgatctc ggtctcctac ctcggcctga ccaatcccgg cgatgagctc gcgatcggca
    25381 agtggcagcc gtggtacgac ctgctgccct gggaggaccg ccgtcccgat gtggccggga
    25441 tcgatgtcac cggcccgctg ctcgcttggg cggacaccga tctggagacg agcacggagc
    25501 gacggcagcg cgtcgcgatc aacttcggcc tcgacgaaca ctcctggcag ccggaactgg
    25561 tgctgcagcg ctatgagttg ctctacgaag ctgggctggt gccggagtcc ccgtccgcgt
    25621 ggcgacggcc ggacgcggaa ctcgcggccg gcgtccgcat gctcggcgat catcggcgga
    25681 ttctcgccac ggcactggcg cggctgcggg ccaagatcac ctatcggccg gtggtgttcg
    25741 aactgctgag cccgaccttc acgctgcgcc aactgcagga ctgcgtcgag gccctggccg
    25801 gacgtgcagt gcacacccag aacttccggc gcctgatcga ccagcaggcg ctggtcgagg
    25861 agaccggcga actggagacg agcaccggcg gacgccccgc gcggctctat cggttccgcc
    25921 gccaggtgct cgccgaacgc cacttctcgg gcaccaagct gccgacgagc cgcgtccgct
    25981 gacgcccctt cgggctggtc gagctgtcga gaccaccacc atcggctgag tccaactggc
    26041 ggactaccct ttgcaccgct tttgctcaga tgtagcataa gtgtatcgag aggggttcct
    26101 atgaccgcca acgtgaccgt ggagaccgcg gccgactgct atccccgcgt cgccaacgtc
    26161 gtcccagccg tcgagtgggc caccatggcc gacgatgtgg acgcgattct gcggctcaag
    26221 cgtgggcgca acgccgtgat cctggcgcac aactacatga cgcccgagat cttccatggt
    26281 gtcgctgaca tcgtcggcga ctccctggcg ctggcacgcg aggccaccac cgtcgaagcc
    26341 gacgtgatcg tgctggccgg cgtccatttc atggccgaaa cagccaagct gctcaacccc
    26401 agcaagaccg tactgatccc cgatctggcc tccggatgct cactcgccga gtcgatcacg
    26461 ccggcggacg tccgcgcact gcggtccgca caccccggcg tgccggtggt cacctacgtg
    26521 aacacctcgg cggcagtgaa ggccgaatcg gacatctgct gcacctccgg caatgccgtc
    26581 cgggtcatcg agagcctgca ggtgccgaag gtgatcatga tcccggaccg cttcctcgcg
    26641 gcgaacgttg ccaggcagac cggggtggag gtgatcgtgc acccgggtgc ctgcgaggta
    26701 cacgagcggt tcacccctgc cgacatcgag caggtacgcg ttgactatcc gggcgtgacc
    26761 gtgctggccc atcccgagtg cccgcccgaa gtcgtggacg tagccgacta cgccggatcc
    26821 accgcccaga tgctgaacta cgttgaagta cgtcgccctg ccaaggtggc cctgatcacc
    26881 gagtgctcga tgagcgacaa cgtcgcccag cagttccccg aactggagtt cgtccggccc
    26941 tgcaatttgt gcccgcacat gaagcgcaac acgctcgcca atatccggta ctcgctggag
    27001 accctgacca atgaggtcat cgtgcccgag gacgtggccg accgggcacg cctcgcggtc
    27061 gagcggatgc tagcggttgg taccggcaaa tgactcacgt tgtggacgca ggctgcccgg
    27121 tcatcatcgg cgcaggtctg gccggcctgg tcgccgctat cgaactcagc ccaatgccct
    27181 gcatcgtgct cagcgccggc gcggtcagca ccggaacttc caccggctgg gctcagggtg
    27241 gtgtggccgc agcggtcggc ccagacgacg accccaccct gcacgcgcag gacaccctcg
    27301 ccgcgggcgc cggactgtgc gaaccggaag ccgtccaagc gatcaccgcg gcagcaccgg
    27361 acgcgatccg ctggctcgcc gagcagggtg cccgcttcga ccacgaggcc gacggcagcc
    27421 tgcggctcgg cctcgaaggc gcgcacagcc gacgaaggat cgtccacgcc cagggcgacg
    27481 gcacgggcgc cgaactgttg cgcaccgtgg tcgacagcgc acgtgaactg agttccgtat
    27541 cgctgtggga ccactccccc gccaccgacg tcatcgtttc gggcggacga gtcgtcggcg
    27601 tccgggtgag cacgccggac ggaccggtgg aagtcctgtc cccgactgtg atcctggcga
    27661 caggcggcat cggcggcctg ttcagccaca ccaccaaccc actcggatcg cgcggtcaag
    27721 ggctggcgct cgcccatcgc gccggtgcca cgctgcgcga cgtggagttc gtgcagttcc
    27781 acccgaccgc gctcgacgtg cacctcgacc cgatgccgct ggtcagcgag gccgtgcgcg
    27841 gcgaaggcgc gatcctggtg aacgagggcg gcgagtgggt gctggcgaac ccgctctcgg
    27901 cccgcgacgt cgtctcccgc gccgaatggg cgcaactcca ggccgggcat cgggtcttcc
    27961 tggacgctcg agagcatccc ggcgagcgct tcgccgcgct cttcccgtcc atccacgcca
    28021 ccgcgctggc cgcaggactc gacccggccc gcgacctact gccggtccgg ccggcggcgc
    28081 attaccacat gggtggcgtg ctggttgagc tcagcggtgc cagcagcgtg tctgggctgt
    28141 acgcggtcgg cgaggtggcc tcgaccggac tgcacggcgc gaaccgtctg gccagcaact
    28201 cgctgctcga agccgtggtg tgcgggcgct ggaccgcaca gcatctgcgc aggacggcca
    28261 acctcacgcc gacgaaggca ccgctgccgg actggcgcac ctggggagac gtgaccggta
    28321 gccggccggc cgatccgcgg gtggccgcgg tgcggcagat cgtcagccgg cacgcgggcg
    28381 tgctgcgcga cgaggatggt ctcgccaccg cgctcaccgc gctccagccc tacctcgaca
    28441 ccgatgccgg gttggtcgcc gaactgttgg tttccgcagc ccgtggacgc accgaatccc
    28501 gcggcgccca cacccgcacc gaccatccgg gaaccgtgga gccggctgag cacacgcaca
    28561 ccgccgccct ggaaggagcc gcccgatgag ggcactgcct cgaatcgtga tcaaaccgct
    28621 ggtgcgcgcc gcgttgctcg aagacctcgg ccgcgcgggc gacctcacca cggacgcgat
    28681 cattgacgcc gaagctaccg ccgaagtcgc cctggtcacc cgcgaaccgg gagtcgtcgc
    28741 cgggttgggt tgtgcggcgc tcgcctggga gctggtcgat ccgcgcatcg ttctcaccgt
    28801 cgaacgaccg gacgggaccc gcgtgcaacc cggtgacgtg atcgctaccg cgtccggccc
    28861 ggccaggggc ctgctcaccg gcgagagggt agccctgaac ttcctcggtc acctctccgg
    28921 cgtagccacc ggcaccgcaa cgatcgcgga cgccatcgcg cacacggcag ccagggtggc
    28981 cgacacccgc aagaccatcc ccggcctgcg cgcgctgcag aagtacgccg tcgtggccgg
    29041 cggcggggcc aaccatcgct tcgggctcga cgacgccgtg ctgatcaagg acaaccacat
    29101 cgcggtcgcc ggtggcgtcc gcgcggcggt gacccgcgtc cgcgcgcacg tgggccacct
    29161 ggtgaaggtc gaagtcgagg tcgactcgct cgcccagttg gccgaactac tggaggtggg
    29221 tgccgacgcc gtcctgctcg acaacatgac gcccgaactg ctgcgcgaag cagtcgcgat
    29281 ggtcgacgga cgcatcgtca ccgaggcctc cggccgaatc acgccggaaa cggcggtacc
    29341 gatcgccgaa gccggtgtcg acctgatctc ggtcggctgg ttgacccact cggcgcgggt
    29401 gctggacatc ggcctcgacc acgtgaccgg tacggcaggc tgatcggttg ttcacctgcc
    29461 ggctgctgct cgtcgatgaa ggcccccgta ctacgacggc gaacgacgac tcaccggacc
    29521 cgacgatcgg cgtttcggcg accctgaacg cacccctgtg cccctgcgca acgtcggccg
    29581 ggtaacaggt tggttcgtcc tgaggttcga ggccatccac cgcggccggc cggccggccg
    29641 gcttgcgccg ggcgcgtcga gcgggcctac agcattccgc tggaagcact gcccgccagc
    29701 aacgcgctgc tccaaaaccg tgcggatacg gcgccaccgc cgcccgaact gtagcgcgcc
    29761 atgcccgagg atcccctcag catcgccgag caccccggcg cgtccatcgg gttcatcgag
    29821 gacgcgcagc gagccgagtg ggcccagctg gggctgggct ggcggcgggg gacgttcgcg
    29881 atcagcggca cctggttcga ggatgcccaa agctacgtgc tcctcctgcg attcgctgga
    29941 gtggctccgc gatcagcacc cgagatattc cgcgccgcgc acctaccagg tggtcgacaa
    30001 ggtgaccggc caaatcacca ccgaatcagc ggctgccgac agcccactgg ctgatcgcct
    30061 gagccgagcc acccgccacc aggacgggcc gtgggcagga ctgtggtggt ccacgcctcg
    30121 aaccggggcg cctgacgagt ttcgccaccg tgacctcgta gcgttacggt gtcccaatga
    30181 ccgctgacac tgtcaacgaa acaagcctgc ccgcagcgcc gaccgacgag tcgcccagca
    30241 ccgattcccg ccaggtggga cccatgttcc cgccgacgag tccgatcgcg ctggcgcccg
    30301 tgacctcacc ggcgttctcg gtagacggct tcgtccgcga attcctgcgt gaactcaaca
    30361 ccggacaggg cgtggccctg tcccgatcca ccatcaacga ccagtacctc gcgctcgcgc
    30421 gcaccgtccg ccactacctg atggcacgct ggctggagac cctgcgacac aaccgcacca
    30481 ccaaggccaa ggtcgtcggc tacctgtcgg cggagtacct gctcggtcgg cagctcggca
    30541 acgcgctgat ggcgtccgac ctcaacgaca tcgccgacaa ggcgatggcc gcctgcggtc
    30601 tcgacctggc caccctccgc gcccaggagg tcgagcccgg cctcggcaac ggcggcctcg
    30661 gtcgcctggc cgcctgcttc atcgactcgc tggcgacgat gagcgtcccc tgcatcggct
    30721 acggcatccg ctacgagtac ggcatcttcc ggcagacctt tgtggacggc cgccaggtcg
    30781 agcagccgga cacctggctc tcactcggcg gtccgtggga gttcccccac ccggaggccg
    30841 cggtccaggt cgacttcggc ggcagcaccg agacctacga cgaccatgga atcgagcgca
    30901 cgcgctgggt gccggcctgg aacgttctgg gcattcctta caactacatg gtgccgggct
    30961 accagaacgg acgcgtcaac accctgcggc tgtggagcgc gcaggcgacc aaggcgttcg
    31021 acctgcacat cttcaactcc ggcgactacg ccgaggccgt gcgggcgcag actttcgccg
    31081 agaacatctc caaggtgctc taccccgagg actcaacccc gcagggcaag gaactccggc
    31141 tgcagcagca gtacttcttc gtcgcatgct cgatccgtaa cttcatcgag gaggtgctcg
    31201 aacaggactt cgacctgcac aacctgtccg agcggatcat cttccagctc aacgacaccc
    31261 acccggtgat cgcggtgcca gagctgatgc gaatcctggt ggacgagaag aagttcgaat
    31321 gggacgaagc ctgggaggtc acccgcaagt gcttcgccta cacttgccac acgctgctgc
    31381 ccgaggcgct cgaagtctgg ccggtcgagc tgatggagcg actgctcccc cggcacatgg
    31441 agatcatcta ccggatcaac gaggacttcc tcgctgaggt gcgtgcggct tacccggacg
    31501 acgaactccg cgcccgccgc atgtcgatcg ttgccgacta cccgacccgc tcggtgcgga
    31561 tggcctacct ggccaccgtg gcgggcacca aggtgaacgg cgtggccgcg ctgcactcgc
    31621 aactgctggc cgacaaggtg ctgcccgact tctccggctt ctggcccgag aagttcacca
    31681 acgtgaccaa tggcgtgacc ccgcggcgct tcctgaagat ggccaacccg tcgctgtcca
    31741 gtctgatcac ggacgcgatc ggtccgacct gggtgactaa cctggaacgg ctgcaggagc
    31801 tggagcccta cgccgaggat gaggagttcc ggagcgcgtt ccgcgaggcc aaggagaaga
    31861 acaaggcccg gctccacgcc ctgctccgcg tccgcgacgg catcgacctg cccggtggcc
    31921 acctgctgga cgtgatggtc aagcggctgc atgaatacaa gcggcagtcg ctgaaactgc
    31981 tgcacctggt gacgctctac gaccagctga tcagcggaac ggtcgacccg gcgacgatca
    32041 cgccgcgcac cgtcgtcttc ggcgccaagg ctgcccctgg ctaccggatg gccaaggaga
    32101 cgatctacct gatcaaccgg gtgggtgcga ccatcaactc cgacccgcgc gtggccggac
    32161 ggttgagcgt ggccttcccg gccaactaca acgtcacgtt ggccgagaag ctgatccccg
    32221 cggcggacct gtccgagcag atctcgctgg ccggcatgga ggcgtccggc accggcaaca
    32281 tgaagttcgc cctcaacggc gcgctgacag tgggcacgga cgacggcgcc aacgtggaga
    32341 tccggcagtt ggtcggcgac gacaacttct tcctgttcgg catggccgag cccgaggtcg
    32401 ctgcactggg tgcggccggc taccagccca ccgcttacta cgaggccaac ccacagctga
    32461 agtcggcgat cgacctgatc tcctcgggcg ccttctcgga cggcgaccgg cacgccttcg
    32521 agcaggtggt atcgaacctg ctctatgagg acaggttcat ggccctggcc gactaccagt
    32581 cctacatcga cgcccaggcc gaggtggaac gcagatacgc cgacgtggac gcctggacga
    32641 agtccgcgat cctgaacgtc gcgcgggccg gcttcttctc ctccgaccgt tcgatgcgcg
    32701 attacctggc ccgtatctgg ggtgcctccc cggagatgtg agccgcaacg gtctctgagg
    32761 aacgagcgtt cctcagagac cgttcatgtc ccgagcggga ccgcgacaag ttgatcgtcg
    32821 tccgcgcccg ggttgccgcg ggcggtgttg ttggtcagca cccagagctg gtcaccgacg
    32881 gcctcgaccg ctcggatccg gcccaggtcg cgcaggaaca cgtccggctc gctgagaccg
    32941 ttcgaggtga gccggctgtg ccagacccgt tcgccgcgga gggccgccag gtagatctca
    33001 ccaaccggtg tgaccgcgat accgctgggg gaagcttcgt cggtcgtcca gacgagcagc
    33061 ggcgcggtga ccccgctcgc gttcgtggcg ccctcggtct gcggccaacc gtagttcgcg
    33121 ccagctttga tcaggttgag ctcgtcatac gcgttctgcc cgaactcact ggcgtagagc
    33181 cgcccggacg cgtcccaccc gagtccctgc acgttgcggt ggccgtagct atagacggcg
    33241 ttgccgaacg ggttgtcgtc cgggatcgag ccgtcgggat gtacccgcag gatcttgccg
    33301 ccgagcgagt ccctgtcttg ggagttccgg gtgttgccgg catcaccggt gccgatgtag
    33361 aggttgccgt cagggccgaa gcgcagccgt ccgccgttgt ggttgccggc cttcgggatg
    33421 ccggtgacga tggggcgcga atcggccagc ccaccgtcgt agcggtaacg caccacccgg
    33481 ttgtcagccg ccgcggtgta gtagaggtac agcagttgat cgctggcgaa ggctggcgat
    33541 agtgcgatgc cgagcagccc gccttcgccg ccaggacgga caccgtccac cttggcgacc
    33601 gtctgcaatt ctccggacga cgcgagccgg accagctcgc cacggtcccg cagggtgatc
    33661 agcacgctct cgtcgggcag ccggacgaag tcccacggca cgtcgagctt cgtggcgagc
    33721 acgcggcgag cagcactcgc cggagccgag ccggtggcgt ccgggctagt cgagacctcg
    33781 gttccgctgg tcgagccacc cactccaccg gtagagccgg tcgagacccc ggacgtgcac
    33841 ccgcagagca gcactccagc gagcagggcg accactatcc ggctcatcgg gcaagtctgc
    33901 ccgcggcgat caagccagcg gccggttctt ggcttcgggt agcagaagtg cggcgcccgc
    33961 ggccagcagg aaggccacgc cgaacacccc gaagaccagc acgagtccgc caccggccag
    34021 gaacagcggg acgctgagcg gagcgaggat cgaagcgatc cgtccgaatg cggccgcagc
    34081 cccggtcccg gtgccgcgca caccggtcga gtagatctcc gggccgatgg cgtacagggc
    34141 accccaggcg ccgagattga agaacgagag cgcagagccg gccgcgatga tggccgcgtc
    34201 cgtgccagcc aacccgaaag ctgcggccga gatcgccgag cccagcagga aggtggccag
    34261 ggtgctgcgc cgtccccact tctcgatcag ccaagcggcg accgcgtaac cgggcagctg
    34321 agcaagggtg atgatcaccg tgaagccgaa cgatttcacc agcgggaagc cgcgttccac
    34381 cagaagggtc ggcagccagg tgaacgctcc gtagtagctg aggttgatca ggaaccagac
    34441 tgcccacagc gcagcggtcc gccgtcgata gaccacagac cagatcgaga gttcgctggc
    34501 cggcacgtgc ggtaccacgt cggggctcgg caccgactcc acgcccgcgg cctgctcgaa
    34561 accgaccacg gcctgctcgg cctcagtgaa gcggcccttg agttccagga agcggacgga
    34621 ttccggcagg ccccagcgca ccacggcggc gtaggcggtg gggatcacgc cgatcgccag
    34681 cgcccagcgc cagccgttcg cgacgttcgg gatgagcagg tagccgatca gtgcggcggc
    34741 gagccaaccc agagcccaga aggcctccag tgccaccacc atgcggccgc ggatccgcct
    34801 cggtgcgaac tcgctgatca gcgtggacgc gaccggcaac tcggcaccga gcccgagacc
    34861 gaccacgaac cgcagggcga tcagggcagc caccccggtc gccagagcgg acgcaccggt
    34921 cgcgatgccg tagaccagca gggtgatggc gaacacctga cggcgtccga tgcggtcggc
    34981 aaggagtccg ccgagggtcg cgccgagggc catgccgacg aagccgatcg agccgatcca
    35041 cgacagcgtg gtggtatcga gctgccactc ggtgcgcagc gcggccatga cgaaggcgat
    35101 caggcccacg tccatcgcgt cgagtgccca accgacacct gagccgacga gcagcttggt
    35161 gtgcttgctg gtgaaaggta gccggtccag gcgctcggcg cgcgtgggtg gctggcgttc
    35221 ggcactggtc attcccggcc cccgtcctta gtcccgggca tcgtagcggc tgctacccgg
    35281 ccgaatcggt agctccggat tgctgagcgg acgtgccttc ctccgcactg gaagcaacgg
    35341 gcgagtcctc gttcggcacg tccccactgg gtgtctctgg gctcggcgac tccgggctcg
    35401 gggttggggt tggagttggc gtcggcgacg gggtcactgc cggggtctcg gtgccggttg
    35461 atgacggcgt attggacgca ggtgcggagc tgtcggttcc ggccgcactg gtgtcgctgg
    35521 cgccggcagc cacgggactg atcggcgccg gcgtgctggt cgtggtactc ggggtcgcgc
    35581 tggacggctt tggcgtccta gtgcttgcgt acgggctcga cgtgtccgtg ccggacggtg
    35641 agggcattcc ctgaggcata cggctgccca cgcggcgact cagggccgcc ttggtctgat
    35701 cgccggtgag gaaacgcatc gggtcgacgt atgcgccgtc caggatcact tcgaagtgca
    35761 tgtggcaagc ggtggagcgt ccggtcgagc cgatccgtcc gatggtttcg gcctgcttca
    35821 cggcctggcc gacggtcacg tcgatctggc tgaggtggct gtaggcggtc tcgaaggtgt
    35881 ggccggagcg gtcggcatgc ttcaggatga tccggttgcc atagccgccg gcccatccgg
    35941 tgtaggtgac cgttccgtcc agtgaggccc aggccgggtc gccacaaggc tggccgagat
    36001 caacgccgtc atgccagcgt tggcggtggg tgaacggatc gctgcggtaa ccgaatccgg
    36061 tgctcggtgt gtggtcggtt accggccagc cgatcaggcc atcgacgtcg gcgagggtcg
    36121 tgccggtcac cacaccgatc gggttggcag tcaggtcgtc agtctcgacg attcccccga
    36181 cgagcgccgc cacggccgtg accgagggct gaaccacctg cacgaccacc ggcgcggcca
    36241 gcggcaccgg agtgggtccg cccggagccg caccagaggg aatcggcagg aggatgtagg
    36301 ccaacggacc gacgacgagg gcgaccaccg tgaccaggag cgcccggcgg ccgatgcggc
    36361 cgatgagtcg gcggagccga cccgaagggc gggcagcaaa tcgcttcccc gcacccatca
    36421 ccggcaccag cccctctgct cgacccgaca aggatcgccc ccggacgggg tggttcccaa
    36481 tagccgattg gagaatctga gcagaatctc aggtggcgct caaggccctc agctctgact
    36541 cagcaatcgc tccctattgg cgcccacctc gccccggggc tccggtggat cgtcgatcag
    36601 accagtgctg cgtccagagt gatggtggtg ccggtgagcg cggcggacac cgggcagcca
    36661 gtcttggcgt cctcagcgaa cttggcgaat tcttccgcgg tcaggccagg gacgcttgcg
    36721 cggaccgtga ggacaatgcc agtgatgccc tggccggcca cgaaggtgac gtcggcgcgg
    36781 gtgtcgaccg ctgcgggcgg cgtgccgttg ccggccaggg cgtgggaaag ggccatcgag
    36841 tagcaggcgg agtgggccgc ggcgatcagc tcctcggggc tggtgcggcc ctcggtctca
    36901 gctgagcgtg agcgccagtc aacctggacg ctgcccaggt tggagttgag gaactcaacc
    36961 tcaccctcac cttcgatgag cgaaccttcc cagtgggcct gagccgagcg aaccgtcatg
    37021 tcgttgcctt tcggagttgg aaaccttgcc gctttgaacc cgagtacccc gcgacgtatt
    37081 cctcgactcg gctagcctca agcaatgagc actcccccac cggtggctgc cagcccggtg
    37141 gatcgtgccc tgctggtgac cggcggggtg gggatcgccg tgttcacggc gctgatcctg
    37201 gcgagcgcct gggtatacga cgcggtggtg gacgcggacg gggtgagcgg tctcgaccgg
    37261 ccggtgctcg actgggctct gggcgtgcgg acgccggcgg cggagtactg ggtgaccgcg
    37321 ttcacgaatc tcggcaagac ggtgccgatg gtgatcctcg gcctgatcct gaccagcctg
    37381 atcgggtggc gctggcggcg tcggacggcc tgggtgctga tgctggtcgc gtccgccggt
    37441 tcggtgacgt tcacgatcgt gagcaaggcg atgatcgcgc gcgcccgtcc gccgctggtg
    37501 gacgcggtgc cgccatacga ggacagcttc tcgttcccat ccggccatac gctgaacagc
    37561 acagtcgtcg cgggaatgct cgcctacctc gtggtctggc tcagtcggac gctatgggtg
    37621 cgcctcctgg cggtgctcgg agcggtgttg tgggcgggcc cgatggggtt gagccgggtc
    37681 ttcctcggcc accactggct caccgacgtg atgttcgcct ggatgttcgg cctgggctgg
    37741 ctggcgctgc tgatcaccgt ccacctggtg gttctcggcg tgcaacgagg gcgtcaagac
    37801 cggatttccc aaaggtccgc ggactaggta aggtaaggaa ccgtcctacg ggacatgggg
    37861 ctatggcgca gttggtagcg cgcttccatg gcatggaaga ggccacgggt tcgaatcccg
    37921 ttagctccac c
//
LOCUS       DAOPRQ010000018        37413 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_858_length_37413_cov_11.479763, whole genome shotgun sequence.
ACCESSION   DAOPRQ010000018 DAOPRQ010000000
VERSION     DAOPRQ010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 37413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37413
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_858_length_37413_cov_11.479763"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(15..641)
                     /locus_tag="PKJ61_03625"
     CDS             complement(15..641)
                     /locus_tag="PKJ61_03625"
                     /inference="COORDINATES: protein motif:HMM:NF037627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOC12948.1"
                     /translation="MTSRRARVRPQDSGLPAMDGRRRVAGLKREEVALLAGVSVDYYG
                     RLERGQLGGVSTSVLDAIADALKLDEAERQHLRDLAQAAAAPERTLRSRTAKTPSVRP
                     SIRALLDGMTGVPAYVRNARADIVGANDLCLALYDGILARDEPFNLARFVFLDGRAHG
                     FFLDWDEVADGGSAVGVARGCTKPLARLTSDKGFHLVAPTGFEPALPP"
     gene            799..1650
                     /locus_tag="PKJ61_03630"
     CDS             799..1650
                     /locus_tag="PKJ61_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOC12949.1"
                     /translation="MRTVTLNNGVEMPILGFGVFQITDPAECERAVSEALAAGYRLID
                     TAASYQNEEAVGRAIAASGIPREQLFVTTKLWIQGAGEGRTRAAFERSLQRLGLDHLD
                     LYLIHQPFGDVYGAWRDMEKINAEGLSRAIGVSNFHPDRLVDLIMHNEVIPAVDQVET
                     HPFHQRVADQELMAVEGVQIESWGPFAEGRNGLFTNPVLSSIAGAHGKSVGQVVLHWL
                     TQRGVVVIPKTVRSERMAENLDVFDFELTDQEMAAIADLDTGASQFFDHRDPAMVKWL
                     SGRTLDV"
     gene            1662..2222
                     /locus_tag="PKJ61_03635"
     CDS             1662..2222
                     /locus_tag="PKJ61_03635"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOC12950.1"
                     /translation="MKTRNLGTLQVSGLSLGGRGMSVHYGPSPERGDLIAFTRTAFDL
                     GVTFFDTAQVYGPFTNEDLVGVALEPLRDWVVIATKFGFDIDPADPRRRAIPKRSDAG
                     MQRAIASGMIKINVSAHLSQIFTGALRGVLAANPAMVDSRNWMAPARSAVEQESASFT
                     GLAASASPGPRLYPDPCPRSGRRPCG"
     gene            2240..2476
                     /locus_tag="PKJ61_03640"
     CDS             2240..2476
                     /locus_tag="PKJ61_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007077707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HOC12951.1"
                     /translation="MDIEITTDFIRLGQLLKYANLVADGAEAKSLIAAGGVRVDGEPE
                     NRRGRQVSVGAVVEVDLPGGPQQVRVVAEASGQE"
     gene            2576..2785
                     /locus_tag="PKJ61_03645"
     CDS             2576..2785
                     /locus_tag="PKJ61_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012924381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6458 family protein"
                     /protein_id="HOC12952.1"
                     /translation="MGLGIGIVLLVIGAVLSFAVRDSVPGVDLSMVGYICMGAGAIAL
                     VLGLVTNTQATRTKHTSVVERHDEH"
     gene            complement(3001..4332)
                     /locus_tag="PKJ61_03650"
     CDS             complement(3001..4332)
                     /locus_tag="PKJ61_03650"
                     /inference="COORDINATES: protein motif:HMM:NF014741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HOC12953.1"
                     /translation="MEEQQRDRLPVAAALGQLRAVLDRVDADLGGATPSALLGEATAA
                     RSLTGRLDALASLLLAKADRANASERSAGTPTSTWLAIDQNLTKREAAGALHRASELA
                     AHPVLGQAAVAGRVGTGQVRAINTVLGGLAAQLDAAQQAQAEELLVGLADSMDSDALG
                     RAAPQVLAKVAPADADELLETKLQREAEAAHHNRSLRFWRQGGSVRFAGSLPKLAGEQ
                     FITLLDAHSEALRRTAVEARDPLYVNATPEQRRADAFTHLLGQAATAQPKPGVGTAKV
                     IVKLDYDQLRKGAAGAGVVGDGIQLSAGELRQACCDAELIPVVLRGGSEVLDVGRSRR
                     LVTPAMRAALIARDGGCVFPGCTTPPSACEAHHVVPWWLGGLTALSNLVLMCHHHHGL
                     VEPAKYYQRDQWQVRISEADRLPEFAPPARHPQAGVWLRHARHRHTERTAA"
     gene            4478..5326
                     /locus_tag="PKJ61_03655"
     CDS             4478..5326
                     /locus_tag="PKJ61_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002548577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOC12954.1"
                     /translation="MRIDLHTHSNVSDGTDTPAELVAKASAALEVVALTDHDTFDGLA
                     EAVAAGVQAGVEVLRGIEISAQHRGASVHLLGYGCLADAPGLAAELAHVREGRAQRVP
                     AMLANLARYGMPVPVDVLARQVGDSPSVGRPHLADAMVELGYVADRREAFDNWLADDK
                     PIFVHRYATELKTALRLIRQAGGVALVAHPWGRGSRAQLPPELLAELAASDLLDGVEV
                     DHQDHDQATRADLRALAAELGLLATGSSDYHGAGKQEHDLGCNLTAPEVLTEIRRRVA
                     ERGGRL"
     gene            5375..6487
                     /gene="trpS"
                     /locus_tag="PKJ61_03660"
     CDS             5375..6487
                     /gene="trpS"
                     /locus_tag="PKJ61_03660"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015070711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOC12955.1"
                     /translation="MTDQPVDAGDQLAASTSEASLRRTEARSAEIEAQIAKDPSGFRV
                     MTGDRPTGNLHLGHYFGSLANRVRIQDAGVETFILIADYQVITDRDGTGPIRERVLGM
                     LADYLAIGLDPSRTTIYAHSAVPALNQLLLPFLSLVTDAELRRNPTVKAELEATGDRP
                     MSGLLLTYPVHQAADILFCKANLVPVGKDQLPHLEQTRVVARRFDERYGRVDPARPIF
                     PQADALLSKASTVLGLDGTKMSKTKGNVIEIGMDADTTAKLIKRAVTDSDRFITYDPA
                     NRPEVSNLVLLVSLCTGREPIEIAGEIGDGGGGGLKKLVTESVNEFFAPIRARRAELV
                     ADPGYLLDVLRRGNAHANEIGNATLDEVRAAMNMTY"
     gene            complement(6558..8054)
                     /locus_tag="PKJ61_03665"
                     /pseudo
     CDS             complement(6558..8054)
                     /locus_tag="PKJ61_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004811067.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
     gene            complement(8287..8760)
                     /locus_tag="PKJ61_03670"
                     /pseudo
     CDS             complement(8287..8760)
                     /locus_tag="PKJ61_03670"
                     /inference="COORDINATES: protein motif:HMM:NF013341.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RIO1 family regulatory kinase/ATPase"
     gene            complement(9201..10034)
                     /locus_tag="PKJ61_03675"
     CDS             complement(9201..10034)
                     /locus_tag="PKJ61_03675"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HOC12956.1"
                     /translation="MSVTVTDLPRRAFVEQIMGLPISIHVRGPEAGSDAIAGLVTDAF
                     AALRADDAMFSTWKPDSPVSRIRDGRDQLRNAHPRIRQVAALCELAGQRTGGAFSAWR
                     PGPDGRIRFDPTGLVKGWAVQQAFDVLTTRLADHGRHDALANAGGDIAVHSTRTDTPD
                     WVIAIEDPRDRSRMLRTLCLRTGAVATSGTAARGEHIADPRTGAPATGLLSATLIGPE
                     LTWADVYATAAFVKGGRALPWLATLEGHAAVLVDREDRISTVAAPGTAGPIRAQASVA
                     R"
     gene            complement(10031..10558)
                     /locus_tag="PKJ61_03680"
     CDS             complement(10031..10558)
                     /locus_tag="PKJ61_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017934750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HOC12957.1"
                     /translation="MRKIIVAVMSTISGLVLLFSYHTSTNSQATEATEPATDVGTEQS
                     APSTPNAAEASGSAPDASESTAAPEPASATESSSAASGSSGTYTGDAVETHWGTVQVQ
                     ITVVDGKITKAAAIEYPTENRKDQQINAYAIPKLNAEAVAAQSARIDAISGATVTSDG
                     YIESLQSALDAAHLG"
     gene            complement(10589..12013)
                     /locus_tag="PKJ61_03685"
     CDS             complement(10589..12013)
                     /locus_tag="PKJ61_03685"
                     /inference="COORDINATES: protein motif:HMM:NF013918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin reductase family protein"
                     /protein_id="HOC12958.1"
                     /translation="MALLTEPAPVRTEPPFAQPVLRHPVSTPGWWRDAVGIATWLSML
                     VVVALWVSGGGAQDLTGWASAITSLGRLTGLISANLLLVQVLLMARIPLVERSYGQDE
                     LARRHRLVGFWSFWLMVAHLVAITVGYALSASENVFVQFWQLVVDYPGMLLALVGTLL
                     LVLVVATSIRNARRKLRYESWHLLHLYAYLGVGLAIPHMLWTGQDFLGNPVATTYWWT
                     LWAAAAGSILVWRIGVPLWRSLRHGLRVTEVVRESPDVVSVTMHGRDLDRLPAQAGQF
                     FNWRFLTGSGWTRSHPYSISAAPDGRSLRVTVKDLGDGSHALAAVETGTRVLIEGPYG
                     RLHPGVRTQPKVTLLASGIGISPMRALLEGLPQQPGAVTLIYRARDDADLVLRGEIER
                     IAQRRGARVFYVLGRRLQGRDSWLPEAAAGLSDAEALRQLVPDIASHDVYLCGAPAWM
                     DAAAAAARECGVPASRIHAERFSW"
     gene            complement(12163..12387)
                     /locus_tag="PKJ61_03690"
     CDS             complement(12163..12387)
                     /locus_tag="PKJ61_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002513382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3107 domain-containing protein"
                     /protein_id="HOC12959.1"
                     /translation="MEIKIGIQHVAREITVETESTAKDVEANLEKALADGSLLAVTDE
                     KGRKVLVPADKIAYVDLGQEKPRTVGFGAL"
     gene            complement(12624..13454)
                     /locus_tag="PKJ61_03695"
     CDS             complement(12624..13454)
                     /locus_tag="PKJ61_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOC12960.1"
                     /translation="MAICPAGHETGQTDFCDVCGLPMDGSQQQPSAETVAAATTINCP
                     NCSTPNVAEALFCEACGYDFTTGVAPGPAHPTAVDSPSPSGADADAEAEATDRAGVSD
                     PEVTNAEPVAPAEASAAPEPAAPTDVPAPAAAATRPVVEWVAELWIDPDWYAAQGSTD
                     PMPSAGLPDIVPLVKESNLIGRVSHSRGIFPDVDCELDNGCSRRHAMLSTDGTRWWIE
                     DLNSANGTFVGAATGMLPAMPIPQGRVELNPGQRIYVGAWTRIVIRKATDDELEAFAG
                     "
     gene            complement(13456..14736)
                     /locus_tag="PKJ61_03700"
     CDS             complement(13456..14736)
                     /locus_tag="PKJ61_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016456644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOC12961.1"
                     /translation="MAEFTAAVYQNEFLPDGGTDVNAIVTVRCTGAGAAGRTGSGDAG
                     EIIAVDTSGSMGVANMNAAKQAAMIALDNILDGTYFAVVAGNHKAYLAYPMVQSGAGM
                     VRMDARTRAEARRAISFFRAEGGTAMGTWLNLCKALFNSIGNLAQKHVILLTDGENHN
                     ETTAQLDSAIANCIGHFQVDARGVGVDWKVAEIRKIAQALLGSVDIIGDPNQLSAVFA
                     ELMRNSMSRGVANAELRVWIPQGAQVLFIRQVSPTVEDLSGRGTPVNALTMSYPTGSW
                     GDEERDYHVAVRLPAKAVGQEQLAARVQLAVGADLLTQGLVKAKWSSDNNLTAQINPE
                     VAHYTGQAELAQMIQEGLSARSAGDERTATTKLGRAVQLATETGNAEATAKLRKVVDI
                     TDARTGTVRLRRAVDKADEMALDTASTKTTRIRS"
     gene            complement(14729..15982)
                     /locus_tag="PKJ61_03705"
     CDS             complement(14729..15982)
                     /locus_tag="PKJ61_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HOC12962.1"
                     /translation="MTEAEWPLWSEEHPVPAQDVQPLEADSAPIGPEQGAAAATAASP
                     EGPSCPVCGAAVSPSASFCETCGGTLTPTVVPPPAVPSGEAFSSQTRRLGERVSALAS
                     CPACGGTVAADGYCQVCGAKAPSARDHFTAAPAAWVGGVCDRGVQHHRNEDAMALWAE
                     EDRAVLVVCDGVSTSIDSDVAALAAARKALDVLVTSTSVGVAGDDSRDAAMAQALVDA
                     AHEANEAVVGTTAPDSTNAASATFAAAVIAADRVHHANLGDSRVYFIGAGQQVLLSVD
                     DSMAQAFIEQGMPRAEAEAMPRAHAITKWLGRDSLDIVPRTGVHELTEPGWLVVCSDG
                     LWNYASEPAELAVQLVAASAATTDPVAVAQALVTWANAQGGQDNVTVALARWGGVPAA
                     AASAGRSSLGEAPNDRPGKEQPANG"
     gene            complement(15979..18282)
                     /locus_tag="PKJ61_03710"
     CDS             complement(15979..18282)
                     /locus_tag="PKJ61_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016669209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOC12963.1"
                     /translation="MKCRQPGCTGTIVDGYCDVCGMPGGPASAPVGRGREGGRPSRQS
                     SGPCRQPGCSGRMVDGYCDVCGSPDMAAPVPEPTTGSTATRTSIQLSSTALGSARVTS
                     FGSRPKRQTTDRLVTGRIGAGLTRVPPAPAIDPSKAVLANPEVPLDARVCPRCGTRVG
                     QGAENVATRSEGYCPTCGAAYSFTVKLHPGDVVAGQYEVIGALAHGGMGWIYIAKDRN
                     VSGRWVVLKGLLNSDDRDALAATIGELRFLARVEHPLIVEIYNFVTHDDASYIVMEYV
                     GGRSLKQLLKQRMTANGGRSDPLPVDQALAFLIEILPALGYLHELGLLYCDFKPDNVI
                     QVGDSLKLIDLGGVRHAGDEDSAIYGTVGFQAPEVATLGPSVASDIFTVGRTLMVLCA
                     EVRGYQSEYEFKIPPAETMPAFAEHDSLYRLLLKCCAPDPSDRFTSAEELRLQMLGVL
                     REVVAAATPGVATTAASSVLFEAPTVSSERYGMRQLPRLRPDQTDPQVGWLAKINADD
                     PIERLSALSAAPGQSPEVQLARGYAALDANNPRVLDECVRQMLTADPWEWRAVWMAGL
                     GALQAQDYANAQSSFNAVYGQVPGELAPKLALAVACELGGEYDIAENLYRICASTDAA
                     YVTPAAFGLARVRAARRDLAGSLAALELVPSTSRGYPESQRLKADHLVRLATDASGLT
                     EAFAAIAAARLDPAVQAQYRALLYAKAEPLAEKGNLKIAGQTMTRPLLRELQVTNYLE
                     LGRLSDDPQVRSAYIDQANSLRPWSLL"
     gene            complement(18279..19406)
                     /locus_tag="PKJ61_03715"
     CDS             complement(18279..19406)
                     /locus_tag="PKJ61_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC12964.1"
                     /translation="MNATAPAAPGQLGVAARPTEVLEYLGELDRWLTERRAELDSLDA
                     QILATGRQSELTSDMALAMALWQAAKTRQGLLLNAWDSGRVGQQELEKLSALIWGRLD
                     TTGVEATQLQSMTVSLPEAGRLCDALVAQLRTRLNTDPGAEQQQIRLRQLRAQLERIR
                     DQVKLEPKALAPAALAKAEALAARTEELAGKRTRGGDIAGLLGSLEMEAAKFERDLIV
                     GSAQRREARDLLAQAREQFARAESNQRAVRTLADRVSAAVFPAPTATVPQLDALGPLP
                     NTRVALIDYARALSTYERELDQVHAQLTKALAEVDATAALLPALQAKAAEHGFAEHPD
                     LVELADLISRRLAQRPLARPVLQQLLTAYSAQLDYLDGGGR"
     gene            complement(19403..20683)
                     /locus_tag="PKJ61_03720"
     CDS             complement(19403..20683)
                     /locus_tag="PKJ61_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC12965.1"
                     /translation="MAAISATSETQSPATQAAPLAVTPTTGSAEGFAPPTPSRTPRLL
                     RQVQVVAALVVGLITALGVWAVLDLRADVAAAPSVVDQYVRVGQIERGLIEAGNQATL
                     AVINTETASGSHATASMAQLTEVSSLLVAAAQARPSDGPQLQQLAAYVMTYSQQLGGV
                     TGLTKTDATARLATADDQLSQLRAGLAQLQGELLSDATAGSWAQNLAWMVVASLVALA
                     VLAWLSWLVARRTHRVLNLGLVAALVAVLVTLAFAVSAQGSAGTAGELARGTELQRMV
                     NVANGTTQLAVARQMQLRAVLQQNWGAAEQDEYSTAFAAASAAADKDDALPGLNGFKN
                     AHTKLADLLSKSDWPAAAKLVLDDADGSLAAAATRFDDAAARASTEAVQAATDATAQA
                     QRELLVPLMVMIAVGILGALFAVLGIAQRIKEYL"
     gene            complement(20689..22302)
                     /locus_tag="PKJ61_03725"
     CDS             complement(20689..22302)
                     /locus_tag="PKJ61_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012923528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOC12966.1"
                     /translation="MKTANLAAALGKLSEAARAAGFDQHAAAEEGLVLSAAIAESNRG
                     AFLQWCAEVGVETSAQTFMDAAVRGRRYRRAPTPLASQAAATTGRAASAYVKALGEVC
                     QSAALLGEASPAALGAASLAAAAQLATHPVSPVPTAPDDAAQREFVRQAPSILNDVLS
                     RLSASQDRLLDVSRLDPQSPGAFPGLGGVALPGIPGTPTTGDPAALPAQPAPVAPAAT
                     EPDAAEPEAEPEPEKTVEEWLAELDELIGLANVKTEIHRQTAILRVDALRTKAGLKSP
                     TITRHLVFTGNPGTGKTTVARLVAGIYKAIGLLSKGQLVEVDRSELVAGYLGQTAIKT
                     AEVAAKAYGGVLFIDEAYSLNGDQYGEEAINTLVKEMEDHRDDLVVIVAGYPAPMEVF
                     ISNNPGLASRFRTTVYFDNYTDEELRSIFTQMAAKSDYDLGDGCLDEFNRQLAEQVRD
                     ETFGNGRYARNLLEAAIGHHAWRLREVNEPTLVELRTLLPGDLEPESVPTPPETPTSE
                     AVPGEPVRLTPPSSGAQISPVEFPAGGES"
     gene            complement(22299..22808)
                     /locus_tag="PKJ61_03730"
     CDS             complement(22299..22808)
                     /locus_tag="PKJ61_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC12967.1"
                     /translation="MGFWDRLLGREDEPAEAEPEIATAPTAHDITASLAGVDELVSDP
                     AVPAVVRARARRVTDTVRKTLPRLELLGLGSYDSYSVVATATDYLPEAISAYLRLPRD
                     WADSRPVEGGKTSLLLLIDQLDLLAVTMDSIYDAANTTDAAALIRHGKFLKERFGHDQ
                     APELNLGQP"
     gene            complement(22813..24036)
                     /locus_tag="PKJ61_03735"
     CDS             complement(22813..24036)
                     /locus_tag="PKJ61_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016456650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HOC12968.1"
                     /translation="MSTPAQPSNQAVQLAPPDMLNLTPPAPPATVTATQAPAIAPQVE
                     AAQVPVLDAKVEDFLTALSTAQQNSPAFSAQADAVRSMGDVDIRKAAETSNRLLDTPV
                     RAIEQGGLADTSKVGKTLMELRRTVENLDPAQAKDQGFWSKLGFGRQVTDYFRKYQSS
                     QTHLNGILHALRSGQDELTKDNVALNMEKQNLWNVMGRLNQYVYIAERLDAKLSAQIA
                     ELEIADPEKAKALNQDVLFYVRQKHQDLLTQLAVSIQSYLAIDIIIKNNIELIKGVDR
                     ASTTTVSALRTAVIVAQALGNQKLVLDQITALNTTTSDMIKRTSEMLKENSAQIQEQA
                     ASSTIGIEQLQAAFANIYATMDSIDQFKLKALDTMSATIGTLETEVAKSRQYLDRVAQ
                     QDTRAASLPAITIDK"
     gene            24213..27329
                     /locus_tag="PKJ61_03740"
     CDS             24213..27329
                     /locus_tag="PKJ61_03740"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HOC12969.1"
                     /translation="MTISTSRTLPALDADQAAVLDGWGAEALLLLGGPGTGKTTVLAH
                     AAALDRPSGAGAAAGGAPLVLAASRTAASHLRNAVTAEFGAGAWQPTVTTVHALSRSL
                     WRRFAERPQLRLLAAPEQEFRVRELLAYGSATRWPDHLRPALGTRGFAAQVRLAIARA
                     RQFGLDPEDLVAFGESAGERGWVGLGRFFAEYLDVLDAEGVLDYAELVHRARLLLAEP
                     AVGAALAAEISAVLVDDFTELDPAQIRFVAAVAGGAPVLATGDPDSVATAFRGAAPRA
                     VVEFQEVFATAARPARVDGLQRGHRLGPATCAALAGLRQRLPRPAGSSLRNLRATDAA
                     DSVTVLTCAGAAEQAAAVAAELARARLEGLSYGDMAVLVRSGRRQLGALVQALVAAGI
                     PVEVAGDEIPLADAPAVRPLLLGLGIAMRDQVTPEEAVRLLTSPLGGFDSIGLRRLAR
                     QWRRTQPEAALIGLPQQLAAAVNEPGWLAGVDTTEAVKLRAVLELVTEARALVADGAA
                     VDVVAWKLWQGTDWPNRLNRESFAGGALGSRADTDLDAVCAFFELAAEADRRGGAGGV
                     RAFLAELAGQQIPADRERESRVRKRGVQVLTAHRARGREWELVVVAGVQEGLWPSGRR
                     MSAVLDAGLLTPDGLTGRTEHRDQLAAERRLFHLACSRARRRLVVTATAGTEGEADAP
                     SRFLAELGVAPQTPQPDAQPLSLGSLTAQLRRAATDPAEPRAMRSAAADELAWLADQS
                     DASGRPLVPAADPANWWGVRELSSLPGATDAQVRLSPSQVTSVLTCPRRYFLSRQAGG
                     EAGVGLAATLGSLIHLLVQQVVTEGLELTDLRSRLDEVWERLPFGARWLSASERVEVE
                     LALQRFLNWRAARKAELIGVEVPFALNLTVGGYDVLLEGKVDWLERTADGLRVVDFKT
                     SRSQPTKAEIAGLEQLGVYQLAIDNGGFDARGAGSVGAAAVYLRQAGRPEDVPKEFGQ
                     ESLKVLPHLSTEPAELAFPTWVHHRVAAAAAVVAEGSYPATPGAHCRACVFATSCPAS
                     ERGRQVAQ"
     gene            27326..30490
                     /locus_tag="PKJ61_03745"
     CDS             27326..30490
                     /locus_tag="PKJ61_03745"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HOC12970.1"
                     /translation="MKLTDPAQLSQALGIPFSEQQLAAITAPLEPSVIIAGAGSGKTT
                     VMAARVVWLVGSGQVRPEQVLGLTFTRKAAGELGGRVRRALLAAGVLDADVEAGEELI
                     LTYDAFAGRLVAEHGLLLGIEASSRLITTAARFRLAARIVAETAGLDHLSRLRPAAVA
                     QRVVELSAELRSHLVEPAQLQAQADNFTRALAAAPRNPRGAEYAALQLAAAATAERLE
                     LAALVQRYEALKLELGYVEFADQMAAAARLAVEVPSVSAALRSEFAVVLLDEYQDTSA
                     AQGTLLRALFSGAEPAQGRGHPVTAVGDPCQAIYGWRGAAASNILDFGAHFPTSDGSA
                     ATSYALTVNRRSGQLVLDAANELAAGVRADPALMAHGLDLDLVAPPNTPPGQVDAASF
                     ATWPDEVAALADRVAELHDTGAVPAWSEIAVLARRNVQVADVYAALSARDIPAEIVGL
                     GGLLELPPVADVVATLTVLDDPTANPSALRLLASTRWAIGIPDLAQLGRRARELAEAK
                     HTAGADSGTADPSKLANTDPAAAPSILEAVADPGPLPYSAAARERFREFEGELRQLRR
                     HSGDAVTELVQRVIAVLGLAVELEAIDPGGSAPLATFVQAVAAYTDIDADASLSGLLA
                     YLEAEREYGTGLEQSVLSAADSVKVLTVHKAKGLEWTAVFVPALAADVFPTDRVSGNW
                     LRNAATLPYPLRGDAAALPQLGVVDNDHFTRFAADLREQQRLSEDRLAYVAVTRARQL
                     LVASTHTWGAGLAKPRVASDYFDVLVSHAQSTALAEDVPTRNPLLGLASVVGWPAVSG
                     GEARTALLAAAARVEQSRATRRTTGDDPQPPSLDADQRERVAAWRRRAEQLLAAELEL
                     GAARTSVGLPEYLSVTGFGRLAKDPLGFARELRRPMPRLVSDAQRWGIAFHSWLEQRF
                     QGQTPLLDGEDDELAGVEVAELRAGFEAGPYANAVPIAVETPFTLVLGGRLVRGRIDA
                     VFAGPDGRPQVVDWKTGDARRADPLQLACYRLAWAELKGLDPDEVDAVFYDLRHSVVV
                     RHPDLPDRPALEKLLTTVPLALPR"
     gene            30487..31458
                     /gene="nudC"
                     /locus_tag="PKJ61_03750"
     CDS             30487..31458
                     /gene="nudC"
                     /locus_tag="PKJ61_03750"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF001299.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HOC12971.1"
                     /translation="MSSPLGPGPASFSIQRRGELLRGPGASWLDHSVLDRVPDNRAET
                     AWVDGLWESADAVVLAAAEAQVAVDSTGLRTEPSAGDYDPESHFLLGLVAGTPWFAAA
                     GGEGLAPLRSVMDSLGTTELELAFTAAGLVGWHARAGYCPTCGAATRAVAGGTVRRCT
                     ACEQEQYPRTDPAVIVAILDDDDRLLLGRQPVWPAGRHSVFAGFVEVGESLEQAVHRE
                     MAEEVGLTLHGLEYLGSQPWPFPRSLMVGFLARADGTEITLAGDEIERARWFTREQLR
                     TALDGEVILPPVTSIARRMIEAWLAGRLTAGAGDPVRCLGTADQRLV"
     gene            complement(31570..33369)
                     /gene="recD"
                     /locus_tag="PKJ61_03755"
     CDS             complement(31570..33369)
                     /gene="recD"
                     /locus_tag="PKJ61_03755"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HOC12972.1"
                     /translation="MIDLATSLRPGLLLDVHASGALSWGDVHVAQKVSHLYGETDQSV
                     QLALALTVRALRAGSTCLDLTTTDQSLAGVDEDAVGVPVELWPQSEPWRAAITASPMA
                     NVGADAPGDRPLRLVGDLLYLERYWQEETEVADELLRRRAEAPAEVEPERLAAAVAEL
                     LDADADADQAVAAIMPALSMVTVIAGGPGTGKTHTLARLLSTLWRTLPRPPLVALAAP
                     TGKAAVRMQEALAEELVRLPEDLAGELGRLRATTIHRLLGWVPESRSRFQHHRANPLP
                     HDVVVVDEASMVNVTLMARLLAALRPQARLVLVGDPNQLAPVEAGPVLADVVAAPATV
                     PAAARAALQLVGAPESDTVVRLRHNYRSVRSIADLATAVLAGDPDETLRLATSGTHGI
                     HFAERAEETELRDVVERTGSAMVAAARAGRVEDALASLEFHRLLCAHRRGPFGVSVWS
                     RLVEDWLAASIEGFDPAQSWYPGRPLLVTANSPDLGLYNGDTGVVARTVGEQLRAFFA
                     RGRELHSVSPFLLDGVQTIHAMTVHKAQGGQYEHVTVVLPPPDSPLLTQELLYTAITR
                     AKQRVTLIGSVESLRRGVQQRARRASGLQARLR"
     gene            complement(33366..36716)
                     /locus_tag="PKJ61_03760"
     CDS             complement(33366..36716)
                     /locus_tag="PKJ61_03760"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOC12973.1"
                     /translation="MTTPTQLALHSLEIPTAHDPAAPLPRGTTVLEASAGTGKTYAIA
                     ALATRYLAEGLVEADRIAVISFSRMASAELRSRVRERLGCTVTALTDHLAGLPVELDE
                     TDRLLADGPPEAVHERIARLHRAIAGLDGAAIMTIHQFCQAMLDELGVLAAQDPQATL
                     VEDLSLVRDQVVNDVYLARYANATPAPPFDLATARLLAREAAELADAPLVPAAATGRA
                     AERLAFAAAVRAELAARKLRLGLYSFDDQLSRLRESLTGPTGAAAAARLRRRCQVVLV
                     DEFQDTDPVQWAILRDTYAGHVPLVLIGDPKQSIYAFRGADVSAYTDAVATAADKLSL
                     LVNHRADAGVVQAVNTLFHGVTLGAGIPVPAVTASHHERRLLPSDFSGWAAAARIRCV
                     TEAEPMLAAQARGRIVADLTAELVSLLDSDVQVVTKDGPRPVVASDVAILVNTNARGR
                     AIADALTAAGVPVAFSGADSIFASHAAKDWLTLLRALEQPRQTATRAAMLTDFVGADL
                     TMLALADETEFVTWASLLQGWARTLNAQGVAALFAAIQASSVPGRPGFAERVLGRPRG
                     ERDLTDFRHLAEILHAQHSDGMRGQTLVSWLAEATDRAAATSDRVRRLETDREAVQIM
                     TVHKAKGLQFPIVLLPEAADLWLPDDDSGAPIAFHADGRRVLDLGGADAPGRAERLAT
                     YAQEEAEERLRGLYVALTRAQSHVTMWWSRTRQNTSAAPLHRLLFRRRDVLGAPAASY
                     PVTEPPGDGHPRELLWLAEAGIGVEESEPISTSPRRDRPSIPDRLVLPKFTREIDQFW
                     RRTSYSGLTEAVHARPPVALGELAWTEDEPAEESPAAAVASAGTPSPMAALPGGTGFG
                     SLVHEVLENLDWHTSDEAELTERLLAAATEACAHFAIPELSAAALAKGLLPSLRTDLG
                     RLTGGLPLAAIPIGDRLSELNFELALGGDAQAATLTATLGDVAATLRRWLPADDPLAD
                     YPDALADPLLAGQALRGFLTGSIDSVFRIPGPTGPRFVVVDYKTNRLGPVDLTLEHYS
                     QAAMAAEMIRTHYPLQALLYCVALHRFLAARLPGYSPEAHLGGVGYLFVRGMGGSGVG
                     PETGVFNWFPPAGLVAELSELLAGRRTS"
     gene            complement(36713..>37413)
                     /locus_tag="PKJ61_03765"
     CDS             complement(36713..>37413)
                     /locus_tag="PKJ61_03765"
                     /inference="COORDINATES: protein motif:HMM:NF037683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC12974.1"
                     /translation="RRAEWLRGEVPPFELGRQLMTEVVQEAERSRRQLPSDLADARLH
                     DLEVLVPVPGVGDVKLVGRVRAHGSQLVQAEFSSLAPRHRLEAWIRLLALAAAIPGEW
                     SARVIGKGKVATYAAPPQPGALTILGKLLAIYSRGLSEPLPAFPRVCMEWSSLRESRR
                     DPLDPKVGMNRLRDRWKWETDEAWALFFEFPELVSTELGDREIPGADPSERLLLGALA
                     SAIWAPLLAAEVTR"
BASE COUNT         5541 a        12516 c        13310 g         6046 t
ORIGIN      
        1 agcacgccgc cctctcaagg cggtagcgcg ggttcgaatc ccgtcggggc tacaaggtga
       61 aaccccttgt cagacgtaag tctggcaagg ggttttgtgc atccacgggc aacgccgaca
      121 gcgctcccgc cgtcggcgac ctcgtcccag tcgaggaaga agccgtgggc gcgaccgtcg
      181 aggaaaacga agcgggcgag gttgaacggt tcgtcgcggg cgaggattcc gtcgtagagc
      241 gccaggcaca ggtcgttggc gcccacgatg tcggcgcgtg cgtttcggac gtaagccggg
      301 acgccggtca tgccgtccag aagggcacgg atgctgggcc gcacggacgg cgtcttggcg
      361 gttcggctcc gcagggtgcg ttcgggcgca gcggcggcct gggcgaggtc gcggagatgt
      421 tgtcgctcgg cttcgtcgag cttgagtgcg tcggcgatgg cgtcgaggac tgaggtggac
      481 acgccgccga gctggccgcg ttcgaggcgg ccatagtagt cgacgcttac cccggcgagg
      541 agggcgacct cttcgcgctt gaggccggca acgcgtcggc ggccgtccat cgcgggtagg
      601 ccggagtcct ggggccggac gcgggcgcgt cggctggtca ggaactcgtt gatctcgcgg
      661 cggttgtccg cccgacgacg ctaccgccgg accgtccgcg cgtaacaggt actggcggtg
      721 cgtggtacgg caggtgcttc cgcgtccgcg cgggaagcgg tgtagtggtt gggcaacagc
      781 ccagcggaag gacaactcat gcgaactgtg accctgaaca acggcgtcga gatgccgatc
      841 ctcggcttcg gcgtcttcca gatcaccgac cccgccgaat gtgaacgagc ggtgagcgaa
      901 gccctcgccg ccggctaccg gctgatcgat accgccgcgt cctaccagaa cgaggaagcc
      961 gtcggacggg cgatcgcggc cagcggtatc ccgcgtgagc agctgttcgt cacgaccaag
     1021 ctgtggatcc agggcgccgg cgaaggcagg acccgtgccg ccttcgaacg ctcgctgcag
     1081 cgactcggcc tcgaccacct cgacctgtac ctgatccacc agccgttcgg cgacgtgtac
     1141 ggcgcatggc gggacatgga gaagatcaac gccgaggggc tttcccgggc gatcggggtg
     1201 tcgaacttcc atccggaccg gctggtcgac ctgatcatgc ataacgaggt gatccccgcg
     1261 gtcgaccagg tcgagaccca cccgttccac caacgtgtcg ccgaccagga gctgatggct
     1321 gtcgagggcg tccagatcga gtcgtggggg ccgttcgctg aaggcaggaa cggtctgttc
     1381 accaacccgg tcctctcctc gatcgccggg gcccacggaa agagcgtcgg acaggtggtg
     1441 ctgcactggt tgacccagcg aggtgtcgtg gtcatcccca agaccgtgcg ttctgagcgg
     1501 atggcggaga atctggacgt gttcgacttc gaactcaccg accaggagat ggctgccatc
     1561 gccgacctgg acaccggtgc cagccagttt ttcgatcacc gcgatccggc catggtcaag
     1621 tggctctccg gccgcacact ggatgtctga ggagccggcg catgaagacc cgcaatctcg
     1681 gcaccctgca ggtgtccggg ctcagccttg gcggcagggg catgagtgtg cactacgggc
     1741 cgtcgccgga gcggggcgac ctgatcgctt tcacccgcac cgcgttcgac ctcggtgtga
     1801 cgttcttcga caccgctcag gtctacggcc cgttcacgaa cgaggatctt gtgggtgttg
     1861 ccctcgaacc gctgcgggac tgggtcgtga tcgcaaccaa gttcgggttc gacattgacc
     1921 cggccgaccc gcgccgccgg gcgatcccca agcggtccga cgcgggtatg cagcgcgcga
     1981 tcgcgtccgg catgatcaag atcaacgttt ccgcgcacct gagccagatc ttcaccgggg
     2041 ccctgcgggg ggtgcttgct gcgaacccgg ccatggtgga ttcacgcaat tggatggcac
     2101 ccgcccggag tgcggtcgag caggaaagtg cgagctttac gggcttggcc gctagcgcgt
     2161 ctcccggacc gcggctctac ccggacccat gcccacggtc cgggcggcgt ccgtgcgggt
     2221 agtgcgccag acttgcgggg tggatatcga gatcactacg gacttcatcc ggttgggcca
     2281 actgctcaaa tacgccaacc tcgtcgctga cggagccgaa gccaaatcgc tgatcgccgc
     2341 gggcggcgtc cgggtcgatg gcgaacctga aaacagacgc ggccggcagg tcagcgtcgg
     2401 tgcggtggtc gaggtggacc tgcccggtgg accgcaacag gtgcgcgtgg ttgccgaggc
     2461 gtcgggccag gagtaacgcc gggtcaaggg tcgcgtaggt tcggccgacg tcgggacggg
     2521 cgtttcgtgc accgcagctg gggtaccggg cgtctgaacc gacgagagga tgatcatggg
     2581 cctgggtatt ggaatcgttc tgctggtgat tggcgccgtc ctcagcttcg cggtgcggga
     2641 ttccgttccc ggtgtagacc tgtcgatggt cggctacatc tgcatgggcg cgggcgcgat
     2701 agccctggtg ctgggcctgg tcaccaacac ccaggccacc cgcaccaagc acaccagcgt
     2761 ggtggagcgg cacgacgagc actgacgtga actgacgggc ttcgtcaggc tcagccagcg
     2821 atgcgctgcc gggttttgtc aggctcggcg agcggtctta cccgggcttc gtcaggctca
     2881 gccaccgatc gggcccgggc ttcgtcaggc tcggccagcg agcgggccgg gcaccgctgg
     2941 gctcggcgag tgggtcggtg ggcaggcttc gcggccggcc caagctgcgg gccggtcggg
     3001 tcaagccgct gttcgctcgg tgtgccggtg gcgggcgtgt ctgagccaca cgccggcctg
     3061 cggatgcctg gcaggtgggg cgaactcggg tagccggtcg gcttcactga tccgaacttg
     3121 ccactggtcg cgctggtagt acttggctgg ttcgaccagg ccgtggtggt ggtgacacat
     3181 caggaccaga ttcgataggg cggtcaaacc acccagccac cagggcacca cgtgatgcgc
     3241 ctcgcacgct gatggtggcg tagtacaccc agggaacacg cacccaccgt cgcgggcgat
     3301 cagcgccgcc cgcatcgcgg gggtcaccag ccggcgcgag cggcccacat ccaacacctc
     3361 gctaccgcca cgcagcacca ccgggatgag ttcggcgtca caacaggcct gccggagttc
     3421 accagccgac agctgaatgc cgtcaccgac caccccggcg ccagcagcac ccttgcgcaa
     3481 ctggtcgtag tcgagtttca cgatcacctt cgccgtgccc acaccgggtt tgggttgggc
     3541 ggtcgccgcc tggcccagca ggtgagtgaa ggcatcggcg cggcgttgtt cgggggtggc
     3601 gttcacgtac agcgggtcgc gggcctccac ggcggtgcgc cgcaacgctt cgctgtgcgc
     3661 gtcgagcagg gtgatgaact gctcaccggc cagcttgggc agacttcccg cgaaccgcac
     3721 cgaaccaccc tgccgccaga accgcagcga ccgattgtgg tgcgccgctt cagcctcacg
     3781 ctgcaacttg gtttcgagta gctcgtcagc gtcggccgga gcgaccttcg ccagcacttg
     3841 aggagcagcc ctgcccaacg cgtccgaatc catgctgtcg gccaggccca ctagcaactc
     3901 ctcagcttgg gcctgctgcg ccgcgtccag ctgagcagcc aatccgccaa gcacggtgtt
     3961 gatcgcccgc acctgaccgg tgcccacccg tccagcaacc gccgcctgac cgagcactgg
     4021 atgcgcagcc agctcactgg cccgatgcaa cgcaccagca gcttcacgct tggtcaggtt
     4081 ctgatcgatc gccagccacg tcgaggtcgg cgtcccagcc gaacgctccg acgcgttggc
     4141 acgatcggct ttggccagca acagcgaggc cagcgcgtcc agccgtccgg tcagactgcg
     4201 ggccgcggtg gcctcaccaa gcagggcaga cggcgtcgcc ccacccagat cggcatccac
     4261 ccgatccagg acggcccgga gctggcccag cgctgccgcc accggcaacc gatcccgctg
     4321 ctgttcttcc atgaaccaag caaatcggtc gggtctgaca gtttcgtccg tcgcagcgcc
     4381 aaaccgagtg tgcgccccga ttctcagtca actttcagga agctggtgtc gaaccctcac
     4441 cggcccgctt gtcagtgggg cttggcagga tgtgcacatg aggatcgacc tgcacaccca
     4501 ttcgaacgtc tcggacggca ccgacacgcc ggccgagctg gtggccaagg cgtccgccgc
     4561 gctggaggtg gtggcactca ccgaccacga cacgttcgac gggctggccg aggccgtcgc
     4621 ggcgggggtt caggccggcg tcgaagtgct ccgcggcatt gagatctcgg cccagcaccg
     4681 tggtgcctcg gtgcacctct tgggctacgg ctgcctggcg gacgcgccgg ggctcgctgc
     4741 cgaactggcc cacgtccgcg aaggccgggc gcaacgggtg ccggcgatgc tcgccaacct
     4801 ggcgcgctac ggcatgccag ttccggtcga cgtgctggcc aggcaggtcg gcgacagccc
     4861 ttcggtcgga cgtccgcatc tcgcggacgc gatggtcgaa ctcggttatg tcgccgaccg
     4921 cagggaggcg ttcgacaact ggctggccga tgacaagccg atcttcgtgc atcgctacgc
     4981 caccgagctg aagaccgcgc tccgcctgat ccggcaagcc gggggagtgg ctttggtcgc
     5041 ccatccctgg ggacggggca gtcgtgctca gctgccgccc gagttactgg ccgagctggc
     5101 cgcgtccgac ctgttggacg gagttgaggt cgaccaccag gaccatgacc aggccacgcg
     5161 tgcagacctg cgcgcgctcg ctgcggaact cggcctgctg gccaccggct cgtccgacta
     5221 tcacggtgcc ggcaagcagg aacacgatct gggctgcaac ctcaccgcgc cggaggtgct
     5281 caccgaaatc cggcgacggg tagccgagcg aggtggccgg ctgtagcggt gtaaagtcac
     5341 tcgtcgatcc cacgaccgaa aaggcagtgc actgatgacc gaccagcccg tcgacgccgg
     5401 ggaccagcta gccgcatcca cttcggaggc gtcgctgcgc cgcaccgagg cgcgcagcgc
     5461 cgagatcgag gcccagatcg ccaaggatcc gtccggcttc cgcgtgatga ccggcgaccg
     5521 gcccaccggc aacctgcatc ttggccacta cttcggttcg ctggcgaacc gcgtccgcat
     5581 ccaggacgcg ggggtcgaaa ccttcatcct gatcgccgac taccaggtga tcaccgaccg
     5641 cgacggcacc gggccgatcc gcgagcgggt gctggggatg ctggccgatt acctggcgat
     5701 cggcctcgac ccgtcccgca ccaccatcta cgcgcactcc gcggtgcctg ccctgaacca
     5761 actgctgctg ccgttcctct ccctggtgac ggacgcggaa ttgcgccgca accccacggt
     5821 caaggccgaa ctcgaagcca ccggcgaccg gccgatgtcc ggcctgctgc tcacctatcc
     5881 ggtgcaccag gccgcggaca tcctgttctg caaggccaac ctggtgccgg tgggcaagga
     5941 tcagctgccg caccttgagc agacccgggt ggtggcgcgg cgtttcgacg agcgttacgg
     6001 ccgggtcgac cccgcccgtc cgatcttccc gcaggcggac gcgctgctgt cgaaggcctc
     6061 gacggtgctc ggccttgatg gcacgaagat gagcaagacc aagggcaacg tcattgagat
     6121 cgggatggac gccgacacga ccgcgaagct gatcaagcgc gccgtgaccg actctgaccg
     6181 gttcatcacc tacgacccgg cgaaccgccc ggaggtgtcg aacctggtgc tgctggtgtc
     6241 gctgtgcacc ggacgcgaac cgatcgagat cgccggggag atcggcgacg gtggtggcgg
     6301 cggcctgaag aagctggtca ccgagtccgt caacgagttc ttcgcaccga tccgtgcgcg
     6361 ccgagctgaa ctggtcgccg accccggtta cctgctcgac gtgctgcgcc gtggaaacgc
     6421 ccacgccaac gagatcggca atgccaccct cgatgaggtg cgggccgcga tgaacatgac
     6481 ctactgacca gttcggcgac agcgcctgga ggtaagaaag tggggccgga tctttcgatc
     6541 cggccccact ttcgcgatca ggaggcgctt tccggctgat tctcggccga tccaccgccg
     6601 gagcggcgac gccggcggcg acgcggacgg ctggcctcgc catccgcagc ggtcgcgggc
     6661 tgggcatccg cagcttcgac cggcacggtc tcgaccgccc cgtccacggg ctcgccgttg
     6721 cgcagccgac gacggttgcg caacggctgg tcaccgcggt ctttgtggtg accgtgcttg
     6781 tctcgatcgc gcttgtcgtg gccgtgcttg tggcccttgt gctcggccga cgactcacgc
     6841 ggggcggcgt ccttgagtcg gcccttcgtg ccttcaggaa tctctaggtc actgaacaga
     6901 tgcgcggacg tggagtaggt ctcctgcggc tccggaaagt cgagttcgag cgccttgttg
     6961 atcaccttcc agcgggtgat gtcagcccag tcgacaaaag tcaccgcgat gcccttggcg
     7021 ccggcgcggc ccgtccggcc gatcctgtgc acgtaggtca tctccgagtc agggcactcg
     7081 tagttgatca cgtggctgac gtcggtgatg tcgatgccgc gcgcggccac gtcggtggcg
     7141 accagcaccg acaccttgcc ctcacggaac ttcttcagcg ccttctcgcg ggcgacctgg
     7201 ttcagatcgc cgtgaatcga ggtcgcctcg aagccgcggt cgaccaattc gtcggccagg
     7261 cgctgggcgg cccgcttggt gcgggtgaag atcatggtgc gagtgcagct cggcgcctgc
     7321 aggatgcgcc cgacgatctc gggcttgtcc aggtcgtgcg cctggtagat gaactgggtg
     7381 gtgtccggca cggtcagttg ggcgtcgtgc gtctccgcgc ggacgttcac cggctggttc
     7441 aggtgcattc gggccagcgc gacgatcgcg gccggcatcg tggcggagaa cagcagtgtc
     7501 tgccgcgact taggcgtctt ggcgatcagc cgctccacgt cgggcaggaa tccgaggtcg
     7561 agcatctcgt cggcctcgtc cagcaccagc accttcacct gcgaaaggtc gagggagcgc
     7621 cggttggcca ggtcgagtag gcgtccgggg gtgcccacta ccacgtccac gccgtcggtg
     7681 agggtctcca gctggctctc gtaaccgacg ccgccgtaga cggtcaacac ccgcgcgcgg
     7741 cggacggacg atgcgtccgc caggtcacgg gtgacctgca gcgcgagctc gcgggtcggg
     7801 cacatcacca gcgcctgcgg tgcgcccggc ttgaccagat cggcgtagcc gggatcggtc
     7861 gggacggcga tccgctgcag caggctgacg ccgaacgcga gggtcttgcc ggttcccgtg
     7921 cgggcctggc cgatcatgtc ggtgccgcca atggcgatcg gaatcgcgag ggactggatg
     7981 cggaacgggt tgacgatgcc cagtttggcc agagcttcgg tgatctcggg aatgacgccc
     8041 aggtcggcga acgtgtgttc ggcgtcggcc tgaggcaggt ttgtctcggt caggatcttg
     8101 ccttcttcat tgactgcagc ccagcaggcg cagtgagtct cggtccgccg gcggcagggt
     8161 ggcgccgctc caggcaggct cgcactttgg tgtgcggcag gctcagtcta gcggcagcgc
     8221 cacggttggc gggactcggg cactgtacga gtgcacggag ccccgtcaac tgggcgctac
     8281 ccaggttcac caggcgtccg ccagtaggtc ggcgaagagt tcctccggat cgaccggcag
     8341 accccgggag gtgaaccagg tcgctgtgtt gacacaatcg cgatgcagga agtcgagcgc
     8401 gttcgggttg gccactagat cgaccagttg cggcacttcg atgatcacca gacgcggctc
     8461 ggctgcgtcc ggttaggcca cgagcaggtt gtacgggctg aggtcgccgt gcacgacgcc
     8521 gatcgcggtc atggcctgca tggctgcaac caactgctcg aacagcggtc ccgtccagct
     8581 ggacgggtag ggcaccggca caccagccgt gaagagccgg ttcagcgccg cccactccgc
     8641 ggctgcccac agcccggcgg cgacggacct gccataggcg gtcccggcgg tcagggctct
     8701 ggagtcgcgg ctgttccgga cgcggcggcc ctcggtgtaa ccggcgtccc ggtggaacat
     8761 ccgatgctcg ctgttgcggt agcgcttggc cgccagcagg cagttggacg cgggctcgcc
     8821 gggaacggca cgctgcaaca ggatgacatc ggcctccttg ccggtcttga gcacgccaag
     8881 gtcggtgtcg atagctgcgg ccgaggtgac cagccactcc ggctacggct ccgggccacg
     8941 gcaggatcgc tcgacgcatt gccacgtgga ccagcgttga ttggcgctca ggtcttcgct
     9001 gagcgcgcga ttgttcaggg tttcccagtc gatgggacgg tactgcgttc tggcaagggt
     9061 gttttctctc tggagagagg cacccgggta aaacctcaga cctcgggagc ctcgactgaa
     9121 ggggcgttgg cgaacgtccc cagcacggtg ggtgacatct cgcgacctcc tgtcaggcga
     9181 gtggcgcgat cagcgccgaa tcaacgtgcc acgctcgctt gggcccggat agggcccgca
     9241 gtgccgggtg ccgcaactgt gctgatccgg tcctcgcggt ccaccaacac cgcggcatgg
     9301 ccttccagag tggccagcca gggcagtgcc cggcctccct tgacgaaggc agcggtggcg
     9361 tagacgtcgg cccaggtgag ttccgggccg atcagggtcg ccgacagcag cccggtcgcg
     9421 ggagctccgg tgcgggggtc ggcgatgtgc tccccgcgtg ctgccgtgcc ggaggtggcc
     9481 accgcaccgg tacgtaggca tagcgtgcgc agcatcctcg agcggtcgcg gggatcctcg
     9541 atcgcgatca cccagtcggg ggtgtcggta cgagtgctgt gcacggcgat gtcaccgccg
     9601 gcgttggcca gggcgtcgtg ccggccgtgg tcagccagcc gggtggtcag cacgtcgaag
     9661 gcctgttgca ccgcccagcc tttgaccagt ccggtcgggt cgaagcggat gcgtccgtcg
     9721 gggccggggc gccacgccga gaaagcgccg ccggtgcgtt gtccggccag ctcgcagagc
     9781 gcggcgacct gccgaatccg gggatgggcg ttgcggagct ggtcgcgtcc gtcgcgaatc
     9841 cggctgaccg ggctgtccgg cttccaggtg ctgaacatgg cgtcgtcagc gcgcagggcg
     9901 gcgaaggcgt cggtgaccag gccggcgatc gcgtccgagc cagcctccgg gccacggaca
     9961 tgaatgctga tcggcaggcc catgatctgc tcgacgaagg ctcgccgggg caggtcggtg
    10021 acggtgacgc tcatccgagg tgggccgcgt cgagggccga ctgcaacgac tcgatgtagc
    10081 cgtcgctggt gaccgtggcg ccggaaatcg catcgattct ggccgactgt gccgcgacgg
    10141 cctcggcgtt cagcttcggg atcgcgtagg cgttgatctg ctggtctttg cggttctcgg
    10201 tcgggtactc gatcgcggca gccttggtga tcttgccgtc caccaccgtg atctgcacct
    10261 ggacggttcc ccagtgggtc tcgacggcgt caccggtgta ggtgccggag cttccggacg
    10321 cagccgacga tgattcggta gccgaggccg gttccggcgc cgctgtcgat tcggacgcgt
    10381 ccggggccga ccccgaagcc tcggcagcgt tcggggtgga cggcgcggac tgctcggtgc
    10441 cgacgtcggt ggcaggttcg gtggcctcgg ttgcctggct gttggtgctg gtgtgatagc
    10501 tgaacagcag caccaggccg ctgatcgtgc tcatcacagc gacgatgatc ttgcgcattg
    10561 gttctctctc tctctcagga ttgctgattc accagctgaa gcgttcggcg tggatgcggg
    10621 atgcggggac gccgcactcg cgggcggcgg ccgcggccgc gtccatccag gcaggcgcac
    10681 cgcacaggta aacgtcgtgg gacgcgatgt cgggcaccag ttgccgcaga gcctcggcgt
    10741 cgctcagccc ggccgcggcc tccggcagcc agctgtcccg gccctggagg cgacggccca
    10801 gaacgtagaa gacccgggcc ccgcgccgtt gggcaatccg ctcgatctcg ccgcgcagca
    10861 ccaggtcggc gtcgtcgcgg gcgcgataga tcagggtgac cgcgcccggc tgctggggca
    10921 ggccctccag gagggcgcgc atcgggctga ttccgatgcc ggacgccagc agggtcacct
    10981 ttggctgcgt ccgcacgccc gggtggaggc gtccgtacgg gccctcgatc agcacccggg
    11041 tgccggtctc gaccgctgcg agggcatgac tgccgtcacc gaggtccttg acggtgactc
    11101 gcagcgagcg cccgtccgga gcggccgaga tcgagtaggg gtgcgaccgc gtccagccag
    11161 aaccggtgag gaaccgccag ttgaagaact ggccggcctg tgcgggcagc cggtcgaggt
    11221 cgcgtccgtg catggtgacc gaaaccacgt ccgggctctc ccggaccacc tcggtcaccc
    11281 gaaggccgtg acgaagactg cgccaaagcg ggacgccgat ccgccaaacc aggatcgaac
    11341 cggccgcagc cgcccacagc gtccaccagt aggtggtggc gaccgggttg ccgaggaagt
    11401 cctggccggt ccacagcatg tgcggaatgg ccagcccaac gccgaggtag gcgtacaggt
    11461 ggagcaggtg ccaggactcg tagcgcagct tgcgacgggc attgcgaatg ctggtggcca
    11521 cgacgagcac cagcagcagg gttccgacca gggccagcag cattccgggg tagtcgacca
    11581 ccaactgcca gaactggaca aacacgttct cgctcgcgct gagcgcgtag ccgacggtga
    11641 tcgcgaccag atgggccacc atcaaccaga aggaccagaa gccgaccagt cggtgcctac
    11701 gggccagctc gtcctgtccg taggagcgtt ccaccagggg gatccgagcc atcaacagga
    11761 cctggaccag caacaggttc gccgagatca ggccggtcaa gcggccaagg ctcgtgatcg
    11821 cgctggccca gccggtcagg tcctgggcac cgccgccgga gacccagagc gcgaccacca
    11881 ccagcatgct cagccaggtg gcgatgccca ctgcgtcccg ccaccagccg ggcgtgctga
    11941 ccggatggcg gaggaccggc tgcgcgaagg gtggttcggt gcgaaccggt gccggctcgg
    12001 tgagaagtgc catgtcgctc cctgtggatc ggatcgttct ccaccacagt gacgtctgat
    12061 acctgcaccc aggttagggt gagctgtgcc gttgctgtga aaggtgacag cttcgacagg
    12121 ctcagccggc gtcttccagc cttggcctgg atccgggctt ggtcagagcg cgccgaaacc
    12181 gacggttcga ggcttctcct ggccgaggtc gacataggcg atcttgtcgg cggggacgag
    12241 caccttgcgg cccttttcgt cggtcactgc caacagggag ccgtcggcga gcgccttctc
    12301 caggttggcc tcgacgtcct tggcggtgga ctcggtttcc accgtgatct cgcgcgccac
    12361 gtgctgaatg ccgatcttga tctccaccgt tgctccttgc tgggtaaccg gaggcccaat
    12421 ctacccacgc gcgctctgca agtgacgccg cgtacgccca gagcgcaacc atgctcgcgt
    12481 ccgccggacc agcttcaagc cagcgatcct gaccttcgcc agggaatctt cgccggcaag
    12541 agtcagaatc gccggcgttt gtgggtgttg gcggtcgtgt ccccattgtc ccgcgctcag
    12601 tgcgctggtg tgggtggccg gattcagccg gcgaaggctt ccaactcatc atcggtggcc
    12661 ttgcggatga cgatgcgggt ccaggcgccg acgtagatgc gctggccggg gttcagttcc
    12721 acgcggccct gggggatcgg cattgccggc agcatgcctg tggccgcgcc cacgaaggta
    12781 ccgttggccg agttcaggtc ctcgatccac cagcgggtgc cgtcggtgct gagcatcgcg
    12841 tggcgtcggc tgcagccgtt gtcgagttcg cagtccacgt ccgggaagat gccgcggctg
    12901 tgcgagaccc ggccgatcag gttgctttcc ttgaccagcg gcacgatgtc gggcaggccg
    12961 gccgacggca tcggatcggt cgatccttgg gctgcgtacc agtcggggtc gatccacagc
    13021 tcagccaccc attccacgac cggacgcgtc gctgccgcgg cgggtgccgg cacgtcggtg
    13081 ggcgccgcgg gctccggtgc tgcggatgcc tcggcagggg ccaccggctc ggcattcgtg
    13141 acctcggggt cggaaacgcc ggcgcgatcg gtcgcctccg cctccgcgtc cgcgtccgcg
    13201 ccgctcgggc tgggcgaatc gaccgccgtg ggatgggccg gtccgggcgc aacgccggtg
    13261 gtgaagtcgt agccgcaggc ctcacagaac agcgcttcag ccacgttcgg ggtgctgcag
    13321 ttggggcagt tgatcgtcgt ggccgcggcc accgtttcag cggacggctg ctgctgcgat
    13381 ccgtccattg ggagtccgca cacatcgcag aagtcggtct ggccggtctc atgtccggct
    13441 gggcagatgg ccatgtcagc tcctgattcg cgtggtcttc gtggaagcgg tgtccagtgc
    13501 catctcgtcg gccttgtcca ccgcccgccg cagccggacg gtgcccgtcc gcgcgtcggt
    13561 gatgtccacg accttacgca acttcgcggt ggcttcggcg tttccggtct cggtggcgag
    13621 ttgcaccgcc cggccgagct tcgtggtggc cgtccgttcg tcacctgccg acctggccga
    13681 caggccctcc tggatcatct gcgccaactc ggcctggccg gtgtaatgcg cgacctccgg
    13741 gttgatctgc gcggtcaggt tgttgtcgga agaccacttc gccttcacca gcccctgggt
    13801 gagcaggtcg gcgcccaccg ccagctgcac ccgcgcggcc aactgctcct gcccgaccgc
    13861 cttggccggc aatcggacgg caacgtggta gtcgcgctcc tcatcgcccc acgagccggt
    13921 cgggtagctc atcgtcaggg cgttcaccgg ggtgccacgt ccgctcaggt cttcgacggt
    13981 gggggacacc tgccgaatga acagcacctg ggcgccctgc gggatccata cccgcagctc
    14041 ggcattggcc acgccacggg acatcgagtt tcgcatcagc tcggcgaaca ccgcgctgag
    14101 ctggttcgga tcaccgataa tgtccaccga gcccagcagt gcctgggcga tcttgcggat
    14161 ctcggcgacc ttccagtcaa cgccgacgcc gcgggcgtcc acctggaaat ggccgatgca
    14221 gttcgcgatc gcagagtcga gctgggcggt ggtctcgttg tggttctcgc cgtcggtgag
    14281 caggatgacg tgcttctggg cgaggttgcc gatcgagttg aacagcgcct tgcagaggtt
    14341 cagccacgtg cccatcgccg tgccaccctc ggcgcggaag aagctgatcg cgcggcgcgc
    14401 ctcggcgcgc gtccgggcgt ccatgcgcac catgccggcg ccggactgca ccatcgggta
    14461 ggcgagatag gccttgtggt tgccggccac gaccgcgaag taggtgccgt cgaggatgtt
    14521 gtccagcgcg atcatggccg cctgtttggc ggcgttcatg ttcgcgacgc ccatcgaacc
    14581 ggacgtgtcc accgcgatga tctcgcccgc gtccccactg ccggtacgtc cggcagcacc
    14641 ggctccggtg catcggacgg tcacgatcgc attgacatcg gtaccgccgt cgggcaggaa
    14701 ctcattctgg tagacggcgg cggtgaactc agccattggc cggctgctcc ttcccgggac
    14761 ggtcgttggg ggcttcccca agactagacc gaccagcgga cgctgcggcg gcgggcactc
    14821 cgccccaccg ggccagcgcg acggtcacat tgtcctgccc gccctgagca ttggcccagg
    14881 tcaccagcgc ctgcgcgact gccaccgggt cggtggtggc cgcgctcgct gcgaccagct
    14941 gcactgccag ttcggccggt tcggacgcgt agttccacaa gccgtccgag cacaccacca
    15001 gccagcccgg ctcggtcagt tcgtggacgc ccgtgcgcgg cacgatatcg agtgagtccc
    15061 ggcccagcca tttggtgatc gcgtgcgcgc gcggcatggc ctcggcctcg gcccgcggca
    15121 tgccctgctc gatgaaagcc tgtgccatcg agtcgtccac gctgagcagg acctgctggc
    15181 cggccccgat gaagtacacc cgggagtcgc ccaggttggc gtggtgcacc cgatccgcag
    15241 cgatcacggc ggccgcgaac gtggccgagg ccgcgttggt gctgtcggga gcggtggtgc
    15301 cgaccaccgc ttcgttcgcc tcgtgtgccg cgtcgaccag tgcttgtgcc atggctgcgt
    15361 cccggctgtc atcgccggcc acgccgactg aggtgctcgt caccagtacg tccagggcct
    15421 tgcgggcggc tgcgagcgcg gcaacatcgg agtcgatact cgttgaaacg ccgtcgcaga
    15481 cgacgagaac cgcgcggtcc tcctcggccc agagcgccat cgcgtcctcg ttgcggtggt
    15541 gctggacgcc gcggtcgcac acccccccga cccaggcggc gggcgcggcg gtgaagtggt
    15601 cgcgggcact gggtgctttc gcgccgcaga cctggcagta gccgtcggcg gccaccgtcc
    15661 caccgcaggc cgggcaggac gccagtgcgc tcacccgctc accgagccga cgggtctggg
    15721 aggagaatgc ctcgccggac gggaccgcgg gcggcgggac gacggttggc gtcagggtgc
    15781 ctccgcaggt ttcacagaac gaggcggacg gcgagactgc agctccgcag accgggcaac
    15841 tcggaccttc cgggctggct gcggttgcgg ctgcggcacc ctgctcgggt ccgattggtg
    15901 cggagtccgc ctcgagcggc tggacgtcct gggctggaac cgggtgttcc tcagaccaga
    15961 gcggccattc agcttcggtc acagcaggct ccagggtcgt agcgaattgg cttggtcgat
    16021 gtacgccgac cgcacctgcg ggtcgtcgct gaggcggccg agttcgaggt agttggtcac
    16081 ctgcagctcc cgcagtagcg gcctggtcat ggtctgcccg gcgatcttca ggttgccctt
    16141 ctcggccagc ggctccgcct tggcatacag cagcgcgcgg tactgcgcct gcaccgctgg
    16201 atccagccgg gcagccgcga tcgccgcgaa ggcttcggtc aggccggagg cgtcggtcgc
    16261 gaggcgcacc agatggtcgg ccttgaggcg ttgcgactcc gggtagcccc gggacgtgct
    16321 cggcaccagt tccagcgcgg ccagcgagcc ggccagatcc cgtcgggccg cccggacgcg
    16381 ggccagcccg aacgccgccg gggtgacgta agcggcgtcc gtggaggcgc agatccggta
    16441 caggttctcg gcgatgtcgt actcaccccc gagttcgcag gccactgcca gcgcgagctt
    16501 gggcgccaac tcgccgggca cctggccgta gaccgcgttg aaggagctct gcgcgttggc
    16561 gtagtcctga gcctgtaatg cgcccagtcc ggccatccag accgcccgcc actcccatgg
    16621 atcggcggtc agcatctgcc ggacacactc gtccagcaca cgggggttgt tcgcgtccaa
    16681 agcggcatac ccgcgggcga gctgcacctc cggcgattgc cccggtgcgg cactgagcgc
    16741 ggatagtcgc tcgatcggat cgtcggcgtt tatcttggcc agccaaccca cctgcggatc
    16801 ggtctgatcc ggacgaagtc gaggcagttg gcgcatgccg tagcgctcag acgagaccgt
    16861 cggcgcctcg aaaagcaccg aggacgcggc cgtggtggcg acgcctgggg tggcagcggc
    16921 gacgacttcg cgcagaacac cgagcatctg cagccggagc tcctccgccg aggtgaaccg
    16981 gtcgctcgga tcgggtgcgc agcacttcag caacaaccgg tagagcgagt cgtgctcggc
    17041 gaaggcaggc atcgtctccg ccggcggaat cttgaactcg tactcggact ggtagccgcg
    17101 aacctcggcg cacagcacca tcagcgtccg gccgaccgtg aagatgtcgg acgcgaccga
    17161 gggcccgagc gtggccacct ccggtgcctg gaacccgacc gtgccgtaga tcgccgaatc
    17221 ctcgtcgccg gcgtggcgga ccccaccaag gtcgatcagc ttcagcgaat cgcccacctg
    17281 aattacgttg tcaggcttga aatcgcaata gagaaggccg agttcgtgca ggtagcccag
    17341 cgccggcagg atctcgatga ggaaggccag cgcctgatcg accggcagcg ggtcggaacg
    17401 gccgccattg gcggtcatcc gctgcttgag cagctgtttg agcgacctgc cgcccacgta
    17461 ctccatcacg atgtagctgg cgtcgtcatg ggtgacgaag ttgtagatct ccacgatcag
    17521 cggatgctcg actcgggcga ggaagcgcag ctcgccgatc gttgctgcca gcgcgtcccg
    17581 atcgtccgag ttcagcaagc ccttcagcac cacccagcgc ccggaaacgt tgcgatcctt
    17641 ggcgatgtag atccagccca tgccaccgtg tgcgagtgcg ccgatgacct cgtactgccc
    17701 ggcgacgaca tcgccggggt gcagcttcac ggtgaacgag taggccgcgc cgcaggtggg
    17761 gcagtagccc tcagagcggg tagccacgtt ttcggcgcct tgaccgactc tggtgccgca
    17821 acggggacag acccgggcat cgagcggcac ctcggggttg gccagtacgg ccttactggg
    17881 gtcgatcgcc ggggcgggcg ggacgcgggt cagcccggcg ccgatacgtc cagtgaccag
    17941 tcgatcggtg gtctgccgct tcggccgact gccgaacgaa gtgacccggg ccgaacccag
    18001 cgccgtggag ctgagctgga tcgaggtgcg ggtcgcggtc gaaccggtag tgggctcggg
    18061 gacgggcgca gccatgtccg gtgagccgca gacgtcgcag tagccgtcca ccatgcgtcc
    18121 ggaacagccg ggctgccgac acggtcccga cgactggcgg gacggccggc cgccttcgcg
    18181 gccgcgtccg accggagcgg acgcgggacc gccgggcatg ccgcagacgt cgcagtagcc
    18241 atcgacgatc gtcccggtgc agccgggctg ccggcacttc accgcccacc tccgtcgagg
    18301 tagtccagct gggccgaata ggcggtgagc agctgttgca gcaccggacg ggcgagcgga
    18361 cgctgcgcca gtcgacggct gatcaggtcg gccaactcga ccaggtcggg gtgttcggca
    18421 aagccgtgtt cggcagcctt ggcctggagc gcaggcaaca gggcagcggt ggcgtccacc
    18481 tcggcgagcg ctttggtgag ctgggcgtgc acctgatcga gttcgcgctc gtaggtggat
    18541 agcgctctcg cgtagtcgat cagcgcgacc cgggtgttgg gcagcggccc gagcgcgtcc
    18601 agctgtggca cggtcgccgt tggtgcgggg aacaccgcgg ctgaaacccg gtcggccagc
    18661 gtccggacgg ctcgttggtt cgactcggcg cgcgcgaact gctcccgggc ctgggcgagc
    18721 aggtcacggg cctcacgccg ctgggctgaa ccgacgatca ggtcgcgctc gaacttggcc
    18781 gcttccattt cgagtgaacc caggagcccg gcgatgtcgc caccacgggt gcgcttcccg
    18841 gcgagctcct cggtgcgcgc ggcgagcgct tcagctttgg ccagcgcggc cggcgccagc
    18901 gctttgggct ccagcttgac ctggtcgcgg atgcgctcca gttgggcgcg cagttgccgc
    18961 agccggatct gctgctgctc cgctccggga tcggtgttca gccgggtgcg cagttgggcg
    19021 accagggcgt cgcacaggcg tccggcctcc ggcagggaga cggtcatcga ctgcaactgc
    19081 gtggcctcga cgccggtcgt gtcgaggcgg ccccagatca gggcggaaag cttctccaat
    19141 tcctgctgtc cgacccggcc ggaatcccag gcgttgagca gcaggccttg gcgggtcttg
    19201 gcggcctgcc acagcgccat cgccagcgcc atgtcggagg tcagctcgga ctgccgtccg
    19261 gtggccagga tctgcgcgtc cagcgagtcg agttcggctc gacgctcagt cagccaccgg
    19321 tcaagctcgc cgaggtactc caacacctcg gtgggtcggg cggctacccc gagttggccg
    19381 ggtgcggcgg gggcggtggc gttcacaggt actccttgat ccgctgggcg atcccgagca
    19441 ccgcgaacag cgcaccgagg atacccacgg cgatcatcac catcaggggg acgagcagtt
    19501 cgcgctgggc ctgcgcggtt gcgtcggtgg ccgcttggac cgcttcggtg gacgctcggg
    19561 cggcggcatc atcgaaccgg gtagcggccg cggccagcga accgtccgca tcatcgagga
    19621 cgagcttggc cgcggccggc cagtcggatt tgctcagtag atcggcgagc ttcgtgtggg
    19681 cgttcttgaa accattgagt ccgggcagcg catcatcctt gtcagcggct gcggacgcgg
    19741 ccgcgaaggc ggtcgagtac tcgtcctgct cggctgcacc ccagttctgt tgaagcactg
    19801 cgcgcagctg catctgccgc gcgactgcca gctgggtggt gccgttagcc acgttgacca
    19861 tgcgttgcag ctcagtaccg cgagccagtt cgccggcagt cccggccgaa ccctgcgcgg
    19921 acacggcgaa cgcgagcgtg accaggacgg caacaagtgc cgcaaccagg ccgaggttga
    19981 gcactcgatg cgtccgccgt gccacgagcc agctgagcca ggccagcacg gcgagcgcaa
    20041 ccagactggc caccaccatc caggccaggt tctgcgccca gctgcccgcg gtggcgtcgc
    20101 ttagcagctc gccctgcaac tgcgcgaggc cggcgcgcaa ttggctgagc tggtcatcgg
    20161 cggtggccag ccgtgcggtc gcgtccgtct tggtgaggcc ggtcacgccg ccgagttgct
    20221 ggctgtaggt catgacgtag gccgcgagtt gctgcagctg tggaccgtct gacggacgcg
    20281 cctgcgctgc tgcgaccaga aggctggaga cttccgtgag ctgcgccatg gacgcggtcg
    20341 cgtggctgcc cgacgcggtc tcggtgttga tcacggcgag cgtggcctgg tttccggctt
    20401 cgatcaatcc acgctcgatc tgcccgaccc ggacgtactg atcgaccaca ctcggcgcgg
    20461 ccgccacatc cgcgcgaagg tccagcaccg cccagacccc cagcgcagtg atgaggccga
    20521 ccaccagtgc ggccaccacc tgcacctgcc ggagcagccg gggcgtccgg ctgggtgtgg
    20581 gcggggcgaa gccctcagcg ctcccggttg tgggcgtcac cgccagcggg gcggcctgag
    20641 tcgccggtga ttgcgtttcg ctggtggcgc tgatggccgc cacgaagctc atgattcgcc
    20701 gccggcggga aactcgaccg gtgagatctg ggctcccgaa ctcggcgggg tcaacctgac
    20761 gggttcaccc ggaacggcct ccgaagtcgg cgtttcaggc ggggtcggca ctgactcggg
    20821 ttcgagatcc cccggcagta gggtgcgcaa ctcgaccagc gtgggttcgt tgacttcgcg
    20881 cagccgccaa gcgtggtgtc cgatagcggc ctccagcagg ttgcgggcgt agcggccgtt
    20941 gccgaaggtc tcatcgcgca cctgctcggc gagttgccga ttgaactcgt ccaggcagcc
    21001 gtcgcccagg tcgtagtcgg acttcgctgc catctgggtg aagatcgagc gaagctcttc
    21061 gtcggtgtag ttgtcgaagt acactgtagt acggaaccgg ctggcgaggc cagggttgtt
    21121 ggagatgaag acttccatcg gcgccgggta accggcaacg atcacgacca ggtcgtcgcg
    21181 gtgatcctcc atctccttga ccagggtgtt gatcgcctcc tcgccgtatt ggtcgccgtt
    21241 gagcgagtag gcctcgtcga tgaacagcac accgccgtac gccttcgcgg cgacctctgc
    21301 ggtcttgatc gccgtctggc caaggtaacc ggccaccagt tcggagcggt cgacctccac
    21361 caactggccc ttgctgagca gcccgatcgc cttgtagatg cctgccacca ggcgtgcgac
    21421 cgtggtcttg ccggtgccgg ggttgccggt gaacacgagg tgccgggtga tcgtagggct
    21481 cttcaggccg gccttggtgc gcagcgcgtc gacccgaagg atcgcggtct gacggtgaat
    21541 ctcggtcttg acgttcgcga gcccgatgag ctcgtcgagt tcagcgagcc actcttcgac
    21601 ggtcttctcc ggttccggct cggcttcggg ctcggctgcg tcgggctcgg tcgcggctgg
    21661 cgctaccggt gcaggctggg ccgggagtgc agccggatca ccagtcgtcg gggtgcccgg
    21721 gatgccagga agcgctacgc cacccaggcc gggaaacgcg ccgggcgact gtgggtcaag
    21781 ccgggacacg tccagcagtc ggtcctggct cgctgagagc ctggacagca cgtcgttaag
    21841 gatggacggt gcctgccgga cgaactcgcg ctgggccgca tcgtccgggg cggtcggaac
    21901 cggggacacc ggatgggtgg cgagctgggc tgcagcggcc aggctggctg cgccgagcgc
    21961 agcggggctg gcttcgccca gcagcgcggc ggactggcag acctcgccga gcgccttgac
    22021 ataggcggac gcggcgcgtc cggtcgttgc ggccgcctgg ctggccaggg gcgtcggggc
    22081 gcgccggtag cgccggccgc ggacggccgc gtccatgaag gtctgggccg aggtctccac
    22141 gccgacctcg gcgcaccact gcaggaatgc gccacggttc gactcggcga tggcggccga
    22201 cagcaccagg ccttcttcgg cggcggcgtg ttggtcgaac ccggcagcgc gagcggcttc
    22261 gctcagcttg cccagcgcgg cggcgaggtt ggcggtcttc atggttgtcc caggttcagt
    22321 tcgggggctt ggtcgtggcc gaaacgttcc ttcaggaact tgccgtgccg gatcagcgcg
    22381 gccgcgtcgg tggtgttcgc ggcgtcgtag atgctgtcca tcgtgaccgc gagcaggtcg
    22441 agctggtcga tcagcagcag cagcgacgtc ttgcccccct ctaccgggcg ggaatccgcc
    22501 caatcgcggg gcagtcgaag gtaggcgctg atcgcttcgg gcaggtaatc ggtggcggtg
    22561 gcgaccaccg aataggagtc gtagctgccc agaccgagca gttccaggcg cggcagggtc
    22621 ttgcgtacgg tgtcggtgac ccggcgagcc cgcgcacgaa ccaccgcagg caccgccggg
    22681 tcggacacca gttcgtccac cccggcaagg gaggcggtga tgtcatgcgc cgttggcgca
    22741 gtcgcgatct cgggctcggc ctcagccggc tcgtcctcgc gtccgagtaa ccgatcccag
    22801 aagcccattt gcctacttgt cgatggtgat ggcgggtaga gaggctgcgc gcgtgtcctg
    22861 ctgtgcgacg cgatcgaggt actggcggct cttcgccacc tcggtctcca gggtgccgat
    22921 ggtcgccgac atggtgtcga gggccttcaa cttgaactgg tcgatcgaat ccatggtcgc
    22981 gtagatgttg gcgaacgcgg cctgcagctg ctcgatgccg atcgtggagg acgccgcctg
    23041 ctcctggatc tgggccgagt tctctttgag catctcggaa gttcgcttga tcatgtcgga
    23101 cgtggtcgtg ttgagcgcgg tgatctggtc gagcaccagc ttctggttgc cgagcgcctg
    23161 cgccacgatc accgccgtgc gcagggctga aacggtggtg gtggacgccc ggtcgacacc
    23221 cttgatcagc tcgatgttgt tcttgatgat gatgtcgatc gcaaggtagc tctggatcga
    23281 caccgccaac tgcgtgagca gatcctggtg cttctgccgc acgtagaaca gcacgtcctg
    23341 gttgagggcc ttggccttct cgggatcggc gatctccaat tcagcgatct gagcggagag
    23401 cttcgcgtcc aggcgttcgg cgatgtagac gtactggttc agccggccca tcacgttcca
    23461 caggttctgc ttctccatgt tgagcgcgac gttgtccttg gtcagctcgt cctggccgga
    23521 ccgcaacgcg tgcaggatgc cgttcaggtg ggtctgcgag ctctggtatt tgcggaagta
    23581 gtcggtgacc tggcggccga agcccagctt cgaccagaag ccctggtcct tggcctgggc
    23641 cgggtcgaga ttctcgacgg tgcggcgcag ttccatcagc gtcttgccga ccttggaggt
    23701 gtcggccagc ccgccctgct cgatggcgcg caccggcgtg tccagcagcc ggttggaggt
    23761 ctccgcggcc ttgcggatgt cgacgtcgcc catgctgcgc accgcgtccg cctgcgcgga
    23821 gaacgccggc gagttctgct gggcggtgct gagtgcggtg aggaagtcct ccaccttggc
    23881 gtcgagcacg ggcacctggg cggcctcgac ttgcggggcg atggccggcg cctgcgtcgc
    23941 ggtgaccgtg gccggcggcg cgggcggggt caggttcagc atgtcgggcg gcgcgagctg
    24001 gacggcctga ttggacggct gcgcaggtgt gctcatcaga tgacctcttc ggttgctggc
    24061 aatatgccaa atgtagcggc ccgcaccgac agcggaaccc ccacttgcat gcccgctgga
    24121 acgggttcgg ggaaccagtg gggacaaccc tggccaaggc gggggttcgg gcccttccgc
    24181 gcactgtcag tggggcgtga ttgcatggtg cggtgaccat ctcgacgagc cgcacgctgc
    24241 cggcactgga cgccgaccag gccgccgtcc tggacggttg gggtgccgag gcgctgctgc
    24301 ttctgggcgg tccgggcacc ggcaagacca cggtgctcgc gcatgcagca gccctggatc
    24361 gacccagcgg ggccggggcc gctgccggtg gggcaccgtt ggtgcttgcg gccagccgca
    24421 ccgcagccag tcaccttcgc aatgcggtca cagccgagtt cggcgcgggt gcctggcaac
    24481 cgacggtgac cactgtgcac gcgctcagcc gcagcctgtg gcgacggttc gccgagcgcc
    24541 cgcagctgcg gctgctggcc gcgcctgagc aggagttccg ggtgcgggaa ctgctcgcct
    24601 acggctcggc gacaaggtgg cccgaccacc tgcgacccgc tctgggtact cgggggttcg
    24661 ccgcccaggt tcggctggcc atcgcgcgcg cccgccagtt cgggctcgac ccggaagacc
    24721 tggtcgcctt cggcgaatcg gccggcgagc ggggctgggt cgggttgggg cggttcttcg
    24781 ctgaatacct cgatgtgctg gacgccgagg gtgtgctcga ttacgccgaa ctggtccacc
    24841 gcgcacggct gctgctcgcg gagccggcgg tcggcgccgc cttggctgcg gagatctcag
    24901 cggttctggt ggacgacttc accgagctcg accccgccca gatccgattc gtggccgcgg
    24961 tggccggcgg ggcgccggtg ctggccaccg gagatccgga cagcgtcgcg accgcgttcc
    25021 gcggggccgc gccgcgagcg gtcgtcgagt tccaggaggt gttcgcgacc gctgcgcgac
    25081 cggcacgggt cgacgggctg cagcgggggc atcgactcgg cccagccacc tgcgcagcgc
    25141 tcgccggctt gcggcagcga ctgccgcgtc cggccgggtc ctcgttgcgc aacctgcgag
    25201 ctaccgacgc ggcggactcc gtgacggtgc tgacctgcgc gggtgcagcc gagcaggccg
    25261 cagcggtcgc agccgagctt gcccgagccc ggctcgaagg gctctcgtac ggcgacatgg
    25321 ccgtcctggt ccgctccgga cgccggcagc tcggtgcgct ggtgcaggcc ctggtggcag
    25381 ccggcatccc ggtcgaggtg gctggtgacg agatcccact ggcggacgcg cccgccgtgc
    25441 gtccgcttct gctcgggcta ggcatcgcga tgcgcgacca ggtgacccca gaggaagcgg
    25501 tgcggttgct gacctcgcct ctgggcgggt tcgactcgat cgggttgcgg cggctggcgc
    25561 ggcagtggcg gcggacgcag ccggaagccg ccctgatcgg gctgccacag cagttggccg
    25621 ctgcggtgaa tgagccgggc tggctggccg gggtcgacac tacggaggcg gtgaagctgc
    25681 gggcggtact ggaactggtg accgaggccc gggccctggt ggccgacggc gctgccgtgg
    25741 atgtggtcgc gtggaagctg tggcagggca ccgattggcc gaaccggctc aaccgggagt
    25801 cgttcgccgg gggcgctttg ggcagccgag cggacaccga tctcgacgcg gtctgcgcct
    25861 tcttcgaatt ggcagccgag gctgatcgtc gcggcggtgc cggtggggtg cgggccttcc
    25921 tggccgagct ggccgggcag cagatcccgg ccgaccggga gcgcgagtcg cgcgtccgca
    25981 agcgcggggt gcaggtgctc accgcacacc gtgcgcgcgg ccgggagtgg gagctggtgg
    26041 tggtggccgg agtgcaggaa gggttgtggc cgagcggacg ccggatgagc gctgtactcg
    26101 acgccggcct gctcacgccc gacggcctca ccgggcgcac cgagcatcgc gaccagctgg
    26161 ccgccgaacg gaggctcttc catcttgcgt gttcgcgggc acgccgccgg ttggtggtga
    26221 ccgcgaccgc tggcaccgaa ggcgaggcgg acgcgccgtc ccggttcctg gccgagctgg
    26281 gggtcgcgcc gcagaccccg caacccgacg cccagccgtt gagcctcggt tcgctcaccg
    26341 cgcagctacg ccgcgctgcc accgatccgg ccgagccccg ggcgatgcga tcagccgctg
    26401 ccgatgagct ggcgtggctg gccgatcagt cggacgcgtc cgggcgtccg ctggttccgg
    26461 cggctgatcc ggccaactgg tggggtgtgc gggagttgag cagtctgccc ggcgccaccg
    26521 atgcgcaggt gcggctcagt ccgagccagg tgaccagcgt gctgacctgt ccacggcgct
    26581 acttcctctc ccgccaagcc ggcggtgaag ccggcgtcgg gctggcagcc accctggggt
    26641 cactgatcca cctgctcgtg cagcaggtgg tcaccgaggg actggaactg accgatcttc
    26701 gcagccggct cgacgaggtg tgggagcggc tgccgttcgg ggcgcgctgg ctgtccgcgt
    26761 cagagcgggt cgaggtggag ctggcgttgc agcgattctt gaattggcgc gccgcccgga
    26821 aggcggagtt gatcggagtg gaagtgccgt tcgcgctgaa cctgaccgtt ggcggctacg
    26881 acgtgctgct ggaggggaag gtcgactggt tggagcgcac cgccgacggc ctgcgcgtgg
    26941 ttgatttcaa gacgtcccgc agccagccga cgaaggccga gatcgccggc ctggagcagc
    27001 tcggggtcta ccagttggca atcgacaacg gtggcttcga cgctaggggt gcgggctcgg
    27061 tgggcgctgc cgcggtctat cttcgccagg ctggacgccc cgaggacgtg cccaaggagt
    27121 tcggccagga atcgctcaaa gtgcttccgc acctgtccac tgagcccgcc gagttggcgt
    27181 tcccaacctg ggtgcaccac cgggtggcgg ccgccgcagc ggtggtggcc gagggcagct
    27241 acccggccac cccaggtgcc cattgccgcg cctgcgtctt cgccaccagc tgtccggcgt
    27301 ccgagcgtgg tcgacaggtg gcccagtgaa gctcaccgat ccggcccagc tcagccaggc
    27361 gctgggcatc ccgttctccg agcagcagtt ggctgcgatc accgcaccgc tggagccgag
    27421 cgtaatcatc gccggcgccg gctccggcaa gacgaccgtg atggccgcgc gagtggtctg
    27481 gctggtgggt tccgggcagg ttcgccctga gcaggtgctc ggcctcacgt tcacccggaa
    27541 ggccgccggt gagctaggcg gacgggtccg ccgggcgctt cttgccgccg gagtgctgga
    27601 cgcggacgtc gaagccggcg aggaactgat cctcacctac gacgccttcg ccggccggct
    27661 ggtcgccgag cacggcttgc tgctcggaat cgaggccagt tcgcgcctga tcactaccgc
    27721 agcccgcttc cggctggctg cccggatagt tgccgagacc gccggccttg atcatttgtc
    27781 gcggctgcgc ccggccgcag tcgcccagcg tgtggtcgag ctctcggcgg agctgcgttc
    27841 gcacctggtc gagccggccc agctgcaggc ccaggcggac aacttcaccc gggccctggc
    27901 cgccgcaccg cgcaatcctc gtggcgccga gtacgccgcg ttgcagcttg ccgccgctgc
    27961 gacggccgag cggttggagt tggcggccct ggtgcagcgc tacgaggcgt tgaaactgga
    28021 gctgggctac gtggagttcg ccgaccagat ggcggcggcc gctcggttgg cggtggaggt
    28081 tccctcggtt tccgcggccc tgcgaagcga attcgcagtg gtgctgctcg acgagtacca
    28141 ggacacttca gcagcgcaag ggacgctgct gcgggcactg ttctccggtg ctgaacccgc
    28201 gcaggggcgc gggcatccgg tgaccgcggt gggtgacccg tgtcaggcga tctatggctg
    28261 gcgcggcgcc gcggccagca acatcctgga tttcggcgcg cacttcccga cctccgacgg
    28321 gtcggcggcg accagctacg cgctcacggt gaaccggcgc agcggccagt tggtgctgga
    28381 cgctgccaac gaactcgcag ccggggtgcg tgccgatccg gcgttgatgg cgcacggcct
    28441 cgacctcgac ctggtcgccc caccgaacac acctccgggc caggtggatg cggcgagctt
    28501 tgccacctgg ccggacgaag tggccgcact ggccgaccgg gtggccgagt tgcacgacac
    28561 cggggcggtg ccggcgtggt cggagatcgc ggtactcgcg cggcgtaacg ttcaggtggc
    28621 tgatgtctac gccgctttat ctgctcgcga catcccggcc gagatcgtcg ggttgggcgg
    28681 gctgctcgag ctgccgccgg tggccgatgt ggtggcgacc ctgaccgtgc tggacgaccc
    28741 gacagcgaat cccagcgccc tgcgactgct cgcctctacc cgctgggcga tcggtatccc
    28801 agacctcgcc cagcttggtc gccgggcccg tgagctggcc gaagccaagc acaccgccgg
    28861 cgccgattcc gggacagcgg acccatccaa gctggcgaac accgatccgg cggccgctcc
    28921 gagcatcctg gaggcggtgg ccgatcccgg gccgctgccg tatagcgcgg ccgcaagaga
    28981 gcgtttccgg gagttcgaag gtgagctgcg gcaactccgc cggcacagtg gcgacgcagt
    29041 gaccgagctg gtgcagcgcg tcatcgctgt gctgggcctg gccgtggagc tggaggccat
    29101 cgatccaggt ggcagcgcac cgctggccac ctttgtccaa gcggtggcgg cctacaccga
    29161 catcgatgcg gacgcttcgc tctcgggcct gctggcctac ctggaggctg aacgtgagta
    29221 cgggacgggc ctggagcagt ccgtgctttc ggccgccgac tcggtcaagg tgctcaccgt
    29281 gcacaaggcg aagggcctgg agtggaccgc ggtgttcgtg ccggccctgg ccgccgacgt
    29341 gttcccgacc gatcgggtct ccggcaactg gctgcgcaac gcggcgaccc tgccgtaccc
    29401 gttgcgcggt gatgcggccg cgctgcccca actcggcgtg gtggacaacg accatttcac
    29461 ccgtttcgcc gccgacctgc gtgaacagca gcggctcagc gaggaccggc tggcctacgt
    29521 ggcggtgacc cgggctcggc aactgctggt cgccagcacg cacacctggg gtgcagggct
    29581 ggcgaaaccg cgggtcgcgt ccgactattt tgacgtgctg gtcagccacg cccaatccac
    29641 ggccctggcc gaggatgtgc ccacgcggaa cccgctcctg ggtctggcct cggtggtcgg
    29701 gtggccggca gtgtccggtg gtgaggcacg aacggcactt ctcgccgcgg cagccagggt
    29761 ggagcaatcc agggcgacgc ggcggaccac cggcgacgac ccgcagccgc cttcactgga
    29821 cgccgaccag cgggagcggg tcgcggcctg gcgaagaagg gctgagcaac tgttggcagc
    29881 ggagctggaa ctgggcgcag cgcggacgtc ggtcgggctg ccggagtatt tgtcggtgac
    29941 cggcttcggc cggttggcca aggacccgct gggtttcgcc cgcgagctgc gtcggcccat
    30001 gccccggctg gtgagcgatg cccagcggtg ggggatcgcg ttccacagct ggctggaaca
    30061 gcgtttccag ggtcagacgc cactgctcga cggcgaggac gacgaactgg ccggcgtcga
    30121 ggtggccgaa ctgcgtgccg ggttcgaagc cggtccgtat gcaaacgcgg taccgatcgc
    30181 agtcgagacg cccttcacgt tggtgctcgg tgggcggttg gtgcgtggac gcatcgacgc
    30241 ggtgttcgcc gggccggacg ggcgtccgca ggtggtggac tggaagaccg gcgacgcgcg
    30301 gcgggccgat ccgctccagc tggcctgcta ccggctggcc tgggcagaac tgaaaggact
    30361 cgacccggac gaggtggacg ccgtcttcta cgacctgcgt cactccgttg tggtgcggca
    30421 ccccgacctg ccggaccggc cggcgctgga gaagctgctc acaaccgtgc cgctagcctt
    30481 gccaaggtga gttccccgct cggtcccggt cctgcttcat tctcaatcca gcgcagaggc
    30541 gagttgctgc gtggccccgg tgcgtcctgg ctcgaccaca gcgtgctgga tcgcgtgccg
    30601 gataatcggg ccgaaacagc gtgggtggac gggttatggg agtcggcgga cgcggtggtg
    30661 ctcgccgcag ccgaggcaca ggtggccgtc gactcgaccg ggctgcggac ggagccaagc
    30721 gccggcgact acgaccccga gtcacatttc ctgcttggtc tggtggcggg cacgccctgg
    30781 ttcgcggccg ctggcggcga gggtctggcg cccttgcgaa gcgtcatgga ctcactcggc
    30841 accactgaac tggagttggc gttcaccgca gccggactgg tcggctggca cgcccgggcc
    30901 ggctactgtc cgacctgcgg ggcggcgacc cgggccgtgg ctggcggcac ggtgcggcgt
    30961 tgcacagcct gcgagcagga gcagtacccg cgcaccgatc ccgctgtgat tgtggcgatc
    31021 ctggacgacg acgaccggct gttactgggc cgacaaccgg tgtggccggc cggccgccat
    31081 tcggtgttcg ccgggttcgt cgaagtggga gagtctctgg agcaggccgt gcaccgtgag
    31141 atggccgagg aggtcgggct caccctgcac gggcttgagt acctgggttc gcaaccgtgg
    31201 ccgttcccta ggtcgttgat ggtcgggttc ctggcgcggg ccgacggcac cgagatcacg
    31261 ctcgccggcg acgagatcga gcgggcacgc tggttcaccc gggaacagct tcggacggcg
    31321 ctcgacggcg aggtcatcct gccgccggtc acctcgattg cccggcgaat gatcgaagcg
    31381 tggctggctg ggcggctcac cgctggtgcg ggtgaccccg tccgctgcct aggaaccgct
    31441 gatcagcgcc ttgtctaggg aaccgctgat catcgccggc caacgctggc tgagcctgac
    31501 gaagcccgta ggcaacgccc gaagcgacga cccttcgtca agctcaggga tcgttgatca
    31561 ctgccttgcc tatcgcagcc gagcctgcaa gccggaggcc cgccgagccc gctgctgcac
    31621 gccgcggcgc agcgattcga ccgagccgat cagcgtcacc cgctgcttcg cccgcgtgat
    31681 cgcggtgtaa aggagctcct gggtcagcag cggggaatcc ggcggcggca gcaccacggt
    31741 cacgtgttcg tactggccgc cctgggcctt gtggaccgtc atggcgtgaa tggtctgcac
    31801 gccgtccagc aggaagggcg aaaccgagtg cagctcccgg ccacgggcga agaacgcgcg
    31861 caactgctcg ccaacggtac gcgccaccac ccccgtatcg ccgttgtaga gccccagatc
    31921 tggtgagttg gcggtgacca gcagtggtcg ccccgggtac caggattggg ccgggtcgaa
    31981 gccttcgatg ctcgccgcca gccaatcctc gaccagccgc gaccacaccg acacgccgaa
    32041 tggaccacgc cggtgcgcac acagcagccg gtggaactcc aagctggcca gcgcatcctc
    32101 gacccgcccg gcgcgggcgg ctgccaccat cgccgacccg gtgcgctcca ccacgtcgcg
    32161 cagttcggtc tcctcggccc gctcggcgaa gtggattccg tgggtccccg acgttgccaa
    32221 ccgcagcgtc tcgtctgggt cgccggccaa cactgccgtg gcgaggtctg cgatcgagcg
    32281 cacggaccgg tagttgtggc gcagccgcac cacggtgtcg gattcgggtg caccgacgag
    32341 ctgcagggca gctcgcgcgg ccgccggcac cgtagccggt gcggccacga cgtcggccag
    32401 caccggacca gcttccaccg gcgccaactg gttcgggtcg cccaccagca ccaaccgagc
    32461 ctgcgggcgc agcgcggcga gaagcctggc catcagggtg acgttcacca tcgacgcctc
    32521 gtccaccacc acgacgtcgt gcggcagcgg gttcgcccgg tgatgctgga accgactccg
    32581 cgactccggc acccagccga gcaggcggtg gatcgtggtc gcccgaagcc ggccgagctc
    32641 gccggccagg tcctcgggca gccggacgag ttcctccgcg agagcctcct gcatgcgcac
    32701 cgcggcctta cccgtcggcg cggccagcgc gaccagcggg ggacggggca aggtgcgcca
    32761 caacgtgctc agcagccggg ccagggtgtg ggtcttgccg gtacccggac cgccggcgat
    32821 cactgtcacc atgctgagcg ccggcatgat cgcggctaca gcctggtcgg cgtccgcgtc
    32881 cgcgtcgagc agttcggcta ccgctgcggc caagcgctcg ggctcgactt ccgcgggcgc
    32941 ctcggcgcgt cgccggagga gttcgtcggc tacctcggtc tcctcctgcc aatagcgctc
    33001 cagatacagc agatcgccga ccagccgcag cgggcggtcg cccggcgcgt ccgcgccgac
    33061 gttcgccatc gggctggcgg tgatcgccgc ccgccacggc tccgattgcg gccagagctc
    33121 gaccggaacg ccgaccgcat cctcgtcgac gccggcgagg ctctggtcgg tggtggtcag
    33181 gtccaggcag gtagacccag cgcgcagcgc gcgcacggtc agcgcgagcg ccagctgcac
    33241 ggactggtcg gtctcgccgt acaggtgact caccttctgc gccacgtgca catcgcccca
    33301 ggaaagtgcg ccactcgcat gcacgtcgag caggagtccg ggccgcaggc tggtggcgag
    33361 gtcgatcatg aggtcctccg tccggcgagt aactcagaga gttcggccac caggccggcc
    33421 ggcgggaacc agttgaacac accggtttcc ggaccaactc cgcttccgcc cataccccga
    33481 acgaacaggt agccgacgcc gcccagatgt gcctcgggcg agtagcccgg gagccgagcg
    33541 gcgaggaagc gatgcagtgc cacgcagtag agcagcgcct gcaacgggta gtgcgtacgg
    33601 atcatttccg ctgccattgc ggcctggctg tagtgctcga gggtcaggtc caccggcccc
    33661 agccggttcg tcttgtagtc caccaccacg aaccgcggcc cagtcggacc gggaatccgg
    33721 aagaccgagt cgatgctccc ggtgaggaac ccccgcaatg cctgccccgc caacagtggg
    33781 tcggccaggg catcggggta gtcggccaac gggtcgtcgg cggggagcca cctgcgaagc
    33841 gtcgcagcca catcgccaag agtggcggtc agggtggctg cttgcgcgtc gccgccgagg
    33901 gctaattcga agttcagctc actgagtcgg tcgccgatcg ggatggcggc cagggggagc
    33961 ccgccagtca ggcggccgag gtcggtgcgc aggctcggca acaacccctt tgccagtgcc
    34021 gctgcgctga gttcgggtat ggcgaagtgg gcgcaggctt ccgtggccgc tgctagcagc
    34081 cgctcggtga gttcggcctc gtcactggtg tgccaatcga ggttctccag cacctcgtgc
    34141 accaggctgc cgaagccggt gccgcccggc agtgcagcca tcggggacgg tgtgcccgcg
    34201 ctggccaccg ctgcggcggg ggactcctcg gccggctcgt cctcggtcca cgccagctcg
    34261 ccgagggcca ccggcggtcg ggcgtgcacc gcctcggtca gccccgaata cgaagtgcgg
    34321 cgccagaact ggtcgatctc cctggtgaac ttcggcagca ccagccggtc ggggatgctg
    34381 ggacggtcgc ggcgtgggct ggtgctgatc ggctcgcttt cctccacgcc gatcccagct
    34441 tcggccagcc aaagcagctc gcgggggtga ccgtccccgg gcggttcggt gaccggatag
    34501 ctcgccgccg gcgcgccgag gacatcccgg cgccggaaca gcagccggtg cagcggtgcc
    34561 gcggacgtgt tctgccgggt gcgcgaccac cacatggtga cgtgggactg ggctctggtg
    34621 agtgccacgt acagcccgcg gaggcgttcc tcggcctcct cctgcgcgta ggtcgcgagc
    34681 cgctcggcgc gtccgggcgc atccgcgccg ccgaggtcga gcacgcgccg gccgtcggcg
    34741 tggaaggcga tcggcgcgcc ggagtcgtcg tcgggtagcc acaggtcggc cgcctcgggc
    34801 agcaggacga tcgggaactg cagtcccttg gccttgtgca cggtcatgat ctgcacggcc
    34861 tcgcggtcgg tctcaagccg gcggacgcga tcactggtcg cggcggcacg atcggtagcc
    34921 tcggccaacc aggacaccag ggtctggccg cgcatcccgt ccgaatgctg ggcgtgcagg
    34981 atctcggcca ggtgccggaa gtcggtgaga tcccgctcac cccgcggacg cccaagcacc
    35041 cgctcggcga accccggtcg gcccgggacg ctgctggcct ggatcgctgc gaacagcgcg
    35101 gccacgccct gggcgttgag agttcgcgcc cagccctgca ggaggctggc ccaggtgacg
    35161 aactcggtct cgtcggccag cgccagcatg gtcaggtcgg ccccgacgaa gtcggtgagc
    35221 atcgccgccc gcgtcgcggt ctggcggggc tgctcaagcg cgcgcagcag cgtgagccag
    35281 tccttggcgg catggctggc gaagatcgaa tccgcgccgg agaacgccac gggcacgccg
    35341 gcggcggtga gggcatcggc gatggcgcga ccgcgggcgt tggtgttcac caggatcgcg
    35401 acgtcactgg cgacgaccgg acgcggcccg tccttggtca caacctgcac atcgctgtcc
    35461 agcaggctca ccagttcagc ggtcagatcg gcgacgatcc ggccccgcgc ctgggccgcg
    35521 agcatgggtt cggcctcggt cacgcaccgg attcgggccg ccgccgccca cccggagaag
    35581 tcggacggga gcagccgacg ctcgtggtgg ctcgcggtca ccgccgggac cgggatgccg
    35641 gcgccgaggg tgacgccgtg gaacagggtg ttgaccgcct gcaccacgcc ggcatcggcg
    35701 cggtggttga ccagcagcga gagcttgtcg gcggcggtgg cgacggcatc ggtgtaggcc
    35761 gagacgtcgg ccccgcggaa ggcgtagatc gactgcttcg ggtcgccgat cagtaccagc
    35821 ggcacgtggc cggcgtaggt gtcgcgcagg atggcccact gcaccggatc ggtgtcctgg
    35881 aactcgtcga ccagtacgac ctggcatcgc cggcgcagtc gagcggccgc ggcggcgccg
    35941 gtcgggccgg tgagcgactc ccgtagccgg gagagctggt cgtcgaacga gtacaggccg
    36001 agccgcagct ttcgtgcggc cagttcggcc cgcaccgccg ccgcgaacgc gagtcgctcg
    36061 gcggcacggc cggtcgccgc cgccggtacc agcggcgcgt ccgccaactc ggcggcttcg
    36121 cgggccagca accgagcggt cgccagatcg aacggtggtg ccggcgtggc gttggcgtag
    36181 cgggcgaggt agacgtcatt gaccacctgg tcgcgaacca gtgacaggtc ctccaccagc
    36241 gtggcctgcg gatcctgggc cgcgagcacc ccgagttcgt ccagcatcgc ctggcagaac
    36301 tggtggatgg tcatgatcgc cgccccgtcc aggccggcga tggcgcgatg cagccgtgcg
    36361 atgcgctcgt gcacggcttc cggcggcccg tccgcgagca gccgatcggt ctcgtcgagc
    36421 tcgaccggta gcccggcaag gtggtcggtg agtgcggtca ccgtgcaacc cagtcgctcg
    36481 cggacgcggg aacgcaactc ggccgaagcc atccggctga agctaatcac cgcgatccgg
    36541 tcggcctcga ccaatccctc ggcgaggtag cgggtggcca gcgccgcgat cgcataggtc
    36601 ttgccggtgc cggcgctggc ttccaggacc gtagtgcccc gcggcaacgg ggcggccggg
    36661 tcgtgtgcgg tcggtatctc cagcgagtgg agcgccagct gcgtcggcgt ggtcatcggg
    36721 tcacctccgc ggccagcagg ggcgcccaga tcgcactggc cagcgcaccc agcagtaggc
    36781 gctcgctggg gtctgcacct gggatctccc ggtcgccgag ttcggtcgac accagttcag
    36841 ggaactcgaa gaacaatgcc caggcttcgt cggtctccca cttccagcga tcgcgcagcc
    36901 ggttcatgcc caccttcggg tcgaggggat cgcggcgact ctcccgcagc gaggaccact
    36961 ccatgcacac gcgggggaac gccggcagcg gttcgctcag cccgcgggaa tagatggcga
    37021 gcagtttgcc gaggatggtc agcgcacccg gctgcggcgg tgcggcatag gtggccacct
    37081 ttcccttccc gatcacccgg gcgctccact ccccgggtat ggcggcggcc aacgccagca
    37141 ggcggatcca tgcctccagc cggtgtcgcg gcgcgaggct ggagaactcc gcctgaacga
    37201 gttggctgcc gtgggcgcgc acccgcccga ccagcttgac gtctccgact ccgggcacag
    37261 ggacgagcac ctccaggtca tgcaaccggg catcggcaag gtcggacggg agctgccgcc
    37321 gcgaacgctc cgcctcctgg accacttcgg tcatcagttg ccgacccagc tcgaacggcg
    37381 gcacctcacc gcgcagccat tcggctcgcc gga
//
LOCUS       DAOPRQ010000019        36776 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_884_length_36776_cov_11.912475, whole genome shotgun sequence.
ACCESSION   DAOPRQ010000019 DAOPRQ010000000
VERSION     DAOPRQ010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 36776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36776
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_884_length_36776_cov_11.912475"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="PKJ61_03770"
     CDS             complement(<1..415)
                     /locus_tag="PKJ61_03770"
                     /inference="COORDINATES: protein motif:HMM:NF017072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HOC12975.1"
                     /translation="MTATVEGRPDRGLTIGVMGRSRNENEHRPTLHPAHLAKQPAEVS
                     GRLYLEKGYGESFSASDAELGQLVAGLRSHAELVAECDVILQPKPVPGDIVELRPGQV
                     FWGWPHCVQDAELTQLAIDRRLTIIALEAMNHWNTD"
     gene            566..3136
                     /locus_tag="PKJ61_03775"
     CDS             566..3136
                     /locus_tag="PKJ61_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016668185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HOC12976.1"
                     /translation="MTVSFPEVTDLGAIPTGDMPGDKVQITPSHVGKAQVIFPELWTQ
                     LQPLLQRPSGRAVVAVCGGSGVGKSETGSLLAYGLNAHGIGAYVLSGDNYPRRIPAEN
                     DAERLHTFRIAGLQGLADAGLVDDDVRAELAALQSDGRDADPAQASVYAWFDTYFQAG
                     RNALADYLGTSAEVDFDQISGILAAFHNGATTLHLKRMGRTPDALWYDDVDVSDVRVI
                     VIEWTHGNNDLLHGVDIPVLLNSTPEETLAHRRARSRDGAVDSPFTTLVLGLEQAKLH
                     SQAAKAKIIVSKDADLLDQAAYARVMGGRQQTAGPMLNVYPDSIGGTLADLVEFVQRP
                     ELAGVFSSAYLLPSVFNTDLDRGFSVIDYELSDLFATRADLEALTAAGIDFKFDFILN
                     HASVLSPQFQDILAKGASSDYADFFIDWNKFWAGHGTMTADGYLQPDPELIKDMFFRK
                     PGLPILMVRFPDGREVPYWNTFYQEVRYTAPDAQDLMRAAGLQYGRAEVLAGRLADAL
                     ASGKRPAEADFSGYESVRDAVVNHVESRRKYLGQMDLNISSPLVWEFYADTLDKLAGY
                     GAQIVRLDAFAYAPKEPGAKNFLNDPGTWELLGKVKDLADARGLQLLPEIHSTYAEGI
                     HETIAGLGFMTYDFFLPGLLIDAFDRRDASTLKRWIGELLAKKIRTVNMLGCHDGIPL
                     LDLKGLLDEERIASLIDTIVGRGGYVKDLHGAKNMYYQVNATYHSALGESDARLLLAR
                     AIQCFMPGKPQVWYLDLFAGKNNHAAVEKAGSGGHKEINRTNLSADDVAEGLAKPVVQ
                     RQLELLRFRNSFGAFGFDATCEVAETASDKLSITWRKDGLWARLDADLASETFEITTS
                     "
     gene            complement(3199..3636)
                     /locus_tag="PKJ61_03780"
     CDS             complement(3199..3636)
                     /locus_tag="PKJ61_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC12977.1"
                     /translation="MTDPERFFREVPRAETAVAAAPTAGWAYWRHAWTRRHTVAALIA
                     LPLLWTLNAGAARWPSEVGTLALLALIAAIGAVVLASFLPVAGSKALSGGVCALAPAC
                     GVFAATMMLNRMAIDPAYSWLSLGLIATAAALRYFGGGTCSTR"
     gene            complement(3710..5227)
                     /locus_tag="PKJ61_03785"
     CDS             complement(3710..5227)
                     /locus_tag="PKJ61_03785"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012919346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102662 - malonate-semialdehyde
                     dehydrogenase (acetylating, NAD+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HOC12978.1"
                     /translation="MTSDVATQAPKRITHLIGHEYWEGTAERTSPVFNPATGEITGSL
                     DLASKNLVDEVVEQADAAAKQWGATSLAKRTKVLFAYRELLNANKQRIAELITAEHGK
                     VTSDALGEVSRGLEVVEFACGIPQLLKGGHSENVSTNVDAYSLLQPLGVVGVISPFNF
                     PAMVPMWFVPVAVAAGNAVVIKPSEKDPSAANAMAELWREAGLPAGVVNVVHGDKEAV
                     DALLDHPKVKALSFVGSTPIAKYVYARGTANGKRVQALGGAKNHMLVLPDADLDLAAD
                     AAVSGGFGSAGERCMAVSVVLATDSIADELVAKIVERMRTLKTGDGSTGCDMGPIVTE
                     AARDRITGYIAAGAEAGATLVMDGRDGPFAGSEDGFFVGPTLFDHVATDMTIYTDEIF
                     GPVLSVVRVSGYQEGLDIINASEYGNGTVIFTNDGGAARRFEVEVEAGMVGINVPVPV
                     PMAYYSFGGWKNSLFGDSHAHGIEGVHFFTRTKAVTRRWPDPSHRGLQLGFPQNS"
     gene            complement(5298..6317)
                     /locus_tag="PKJ61_03790"
     CDS             complement(5298..6317)
                     /locus_tag="PKJ61_03790"
                     /EC_number="3.5.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008362227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008660 -
                     1-aminocyclopropane-1-carboxylate deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0042217 - 1-aminocyclopropane-1-carboxylate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-aminocyclopropane-1-carboxylate deaminase"
                     /protein_id="HOC12979.1"
                     /translation="MRLQEFPRYPLTFGPSPVQHLKRLSQHLGGAQVWAKREDVNSGL
                     AFGGNKVRKLEYIVPDVLASGADTLVSIGGYQSNHTRQVAAVAAHLGLKCRLVQEQWV
                     PWDDPTNTQVGNILLSRMMGADSRLDPSGFDIGIRDSWKNALAEVEASGGKPYAIPAG
                     ASEHPLGGLGFANWAFEVAEQEQQLGVHFDTIIVCTVTGSTHAGMIAGFAALEELTGV
                     RRRVLGIDASATLAKTRDQVARIARHTAELIELGRDVRDDEITVLEGWAGDLYGIPVD
                     STMAAIRLGAELEAMITDPVYEGKSLAGLIDLVSSGEISRDSNVLYAHLGGQPAINAY
                     HRLWS"
     gene            complement(6430..7404)
                     /locus_tag="PKJ61_03795"
     CDS             complement(6430..7404)
                     /locus_tag="PKJ61_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017935243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HOC12980.1"
                     /translation="MTGIDLPLAELQRYVCDEPAPADFDSVWTQTLQVAREVPMLPRV
                     ERVETGLRLVESYDVTFPGFGGDPIKAWLTVPAGADGPLPVVVGYQGYGGGRGLPLEH
                     ITWANAGYAELFMDSRGQGSSWGSGGHTPDPHGSGPAFPGYLTLGVQSFETLYYRRLI
                     TDAVRAVDAARVLPQVDPERVVVTGISQGGGLTLAVAGLADGLAAVMPDVPFLCNFRR
                     AVDVAETDPYVEIARYLKVHRASVDEVFGVLAYADGVHHAARASAPALFSVAIADQIC
                     PPSTVYAAYHAYAGAKSLEVYPFNDHEGGQTVQVARQLEWLESIGLAP"
     gene            complement(7404..8624)
                     /locus_tag="PKJ61_03800"
     CDS             complement(7404..8624)
                     /locus_tag="PKJ61_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008358189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HOC12981.1"
                     /translation="MITNWIELPAHRLWLARHFQHLLEFGRRTPHPSGGAAWLTDAGV
                     PWLDQGVQTWITCRTVHVYCLGALAGRPGSRPVAAAALAGLTGVLHDTESGGWFSAVA
                     PDGSTPDEKAAYTHAFVVLAASSATAAGLEGAAELLAEALAVFEERFWDEASGRSVDT
                     WNRDFTVLDPYRGINANMHTVEAFLAAADVTGDRVWLDRASRVAAFAVAQAEANEWRL
                     PEHYDSDWTPQLELNVDRPDDKFKPYGATIGHGLEWARLLLHLEAAGATGVEWLPAAA
                     ALFDRAIADGWHADGADGFVYTTDWSGRPVVRDRMHWVTAEAIGASAALFRRTGNHTY
                     SAHYARYWDYAERFVLDHENGSWHHQLDPQNRPADSVWPGKADLYHAAQATLIPTLPL
                     APSLATALARGLHA"
     assembly_gap    8800..8899
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8992..10101
                     /locus_tag="PKJ61_03805"
     CDS             8992..10101
                     /locus_tag="PKJ61_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC12982.1"
                     /translation="MAFPDARAAYAALANRSATLADLRQIVTDFPELRPWVAAYPGTD
                     EALLGWLAALQDPQVDAELATRNAGAAAWSAPVTTGSGVAPSIFAPASPVTNAQAAAP
                     AYQAQPGLPGRTTKRFGWPLVVGAVIVLGLVAGGLSAAFATGMFAPKAASGTSSAGPV
                     RTASAVPSEAETASQVATPGESGAASRFVCWDGAESDTAGDCILPEGKDEYWSFLTYA
                     FPSIASQPGCTEADSTGKSTYVGVTVMWECELGDALIRYRYWEVPSDAEEHYARKFNA
                     KTVLKTYDLLVGGAPTEGWAKTDKDTVDGPAGVQRVVLTIWLPEDQLSVSVEGDTSAA
                     MWAALEQVRTRPGGQMYGHPAQERAVEAPITVEER"
     gene            10124..11143
                     /locus_tag="PKJ61_03810"
     CDS             10124..11143
                     /locus_tag="PKJ61_03810"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF024744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HOC12983.1"
                     /translation="MTNDYRPRRAESSRGGWPRLGAVGVLAALLVLGILGFALLRPVP
                     ATAPTPVAGSAAASATSASVVASGTAVASAAVSETQTPTPEVSREAVTVSSVPNFRDV
                     AGVGIELADGATMATGVVYRAAKLSTLSKADAAKLADAGVSDIFDLRTPDVARRSPDV
                     KIEGATNRLVNLYALDRAPSPPRTNAAAAKDFMRGLYVGFVDDADQRKRIASVLKEVA
                     QVEQPVIVHCSEGKDRTGWVSAMLQFVAGADRETVLEQYLLSNQEREALIAKSYAAAK
                     RTSGVAAADIDRALKTVEASYLNAGLAQVEKRYGSVDGYLSKGLGLKQETLDALRAKL
                     RVEAR"
     gene            complement(11252..11641)
                     /locus_tag="PKJ61_03815"
     CDS             complement(11252..11641)
                     /locus_tag="PKJ61_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC12984.1"
                     /translation="MADSYVFVANGDSCPICAGLDGSFVPAGFKAHAGCTCNTVKMDD
                     GERTCEFEYSENNTYFNGSYRGFRVQLDLTITCPDGSTHTATGYDVGLDTLSTGDSDD
                     TLEDWAHEACDQFCDQAGSPGDEFLCC"
     gene            complement(11651..12769)
                     /locus_tag="PKJ61_03820"
     CDS             complement(11651..12769)
                     /locus_tag="PKJ61_03820"
                     /inference="COORDINATES: protein motif:HMM:NF025589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOC12985.1"
                     /translation="MSALLASTGFRQCEKYADAIVAVRCNGEISSIAEAPMALISDIP
                     TITATQASALESVTLFTLDDLLRTERGALVRRLPQITLTEIRGWQAIAELSQLDTITV
                     AEAAALQAAGVSGIDEFSDWDLARLRPILTQRDDEQLLALAKGSVRLRHTGVVNGNVR
                     LRDETPVEGARVTVAGRDAVTDARGRFRVIGLPLDNRVAVAATHPQLGHKLLTAVPVR
                     RTGALVAVTLVLGGRPRAPRRYSQRRGDTLPPLGSSVITTRVESTPPETDDLLVISGR
                     YARGDYRVASEFLDFEDGVFVRRLYRLPAASVPAAAVVSDGLGWRNDAWTIRKHCAKD
                     VARRVRLRAAARRLSGTPGSAAEASAQARAILAAASDR"
     gene            complement(12773..13393)
                     /locus_tag="PKJ61_03825"
     CDS             complement(12773..13393)
                     /locus_tag="PKJ61_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015071028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOC12986.1"
                     /translation="MIRIAMADDHPIVRSGLSSLLQGMDDVTVVAEHGSAEALLTWLD
                     DNVCDLVLLDLQFGKGRLTGAEATRRISASGGPAVLILTTYATDADILAALDAGARGY
                     LLKDTPTDELERAVRAAAVGQPALSPAVQQRLLQRVLDPATTLTQRELEVLQRAAGGL
                     SNHQIAAELFVTVATVKTHLAHSYAKLGAPSRTAAIVIARERGLID"
     gene            complement(13390..14574)
                     /locus_tag="PKJ61_03830"
     CDS             complement(13390..14574)
                     /locus_tag="PKJ61_03830"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOC12987.1"
                     /translation="MELGSERVTAHLLVGLDLLAALLLVVAVASPSGAAWTVWAAGAV
                     FAFGWLVVRLSVRVLGEPMEARGGWWPGGAAASGLLAAYCLVLLTSESGMWLAFPVML
                     LQLHLLGPRHGVVAVTLTTVAAVVLGAVVRGQVALGFVLGPVIGAFFAVAAVVGLESL
                     ARLVVQRQRALHELEDAQQRLLDAERERLRAEERANLARDIHDTLAQDFAAIDLHLRR
                     TAALMGTDSPAAPAVALARQATAEGLAQARRFIAGEPGHRQDATMGAIQHAAERAQAD
                     SGGRTKVAVRSMGTEPVLSAELSTEILRMTQSALANVVQHAAAANASVSLTWEPDRVL
                     LDITDDGRGFNPDGEEAVGGFGLPALRARVRELGGTVGVESEPGEGTAIAISLPLGSE
                     SA"
     gene            14730..15155
                     /locus_tag="PKJ61_03835"
     CDS             14730..15155
                     /locus_tag="PKJ61_03835"
                     /inference="COORDINATES: protein motif:HMM:NF022521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2871 family protein"
                     /protein_id="HOC12988.1"
                     /translation="MRRIYLAAIVYAALGLLGGLGYREVTHARDFTGYTQLAVLHTHL
                     LALGMLFMLIVLALDATLGISGSRSFSWFFTTYNAGLVITAGALTWHGLLQVFGQAAG
                     AAIAGIAGLGHILLTIGIGCLLVAINKPLRARLADAANV"
     gene            15158..15550
                     /locus_tag="PKJ61_03840"
     CDS             15158..15550
                     /locus_tag="PKJ61_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsmA family protein"
                     /protein_id="HOC12989.1"
                     /translation="MLALAIILISLALAFYTLGVWAERRTGELRWWHVAAFAAGLTAD
                     VSGTVVMTMIANGGGATGVEQSPVLAQLMAATGLVALLLMALHLGWAVVTMIRDRFDE
                     KRVFHRFSIVVWVIWLIPYFTGMAAAMA"
     gene            complement(15884..16117)
                     /locus_tag="PKJ61_03845"
     CDS             complement(15884..16117)
                     /locus_tag="PKJ61_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC12990.1"
                     /translation="MRSDTRYKHGPFIPAACTPTTPTATSPVPTGTTPAATTPTSSTS
                     ADAPTRMVVVYFGNTVLDPEVDCSKVWGAARTH"
     gene            16130..16408
                     /locus_tag="PKJ61_03850"
     CDS             16130..16408
                     /locus_tag="PKJ61_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004085174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOC12991.1"
                     /translation="MIVSFRHKGLEQLYRTGSKKGVQTAHVPKLLRILSLLDVAQAAD
                     DLNIPSFRTHPLKGELAGHWSIWVNGNWRVTFRFVDSDVELVDYQDYH"
     gene            16423..16710
                     /locus_tag="PKJ61_03855"
     CDS             16423..16710
                     /locus_tag="PKJ61_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010893244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HOC12992.1"
                     /translation="MVMQNPVHPGEILREDVLADLGLSVGEAASRLGISRVTLSRVLH
                     GHARISPNLAVRLEVAGVGTARAWLAMQSAHDLAAERAAGLPTVTRLDPVA"
     gene            complement(17321..17977)
                     /locus_tag="PKJ61_03860"
     CDS             complement(17321..17977)
                     /locus_tag="PKJ61_03860"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HOC12993.1"
                     /translation="MLPFHRRLAEIGLAAIGEFGFVLAGGYAISANGMGDRPSEDVDL
                     FTNRSDPEQFATAVDRLREAYVAAGLRVEDGRLRATFAEFDVTDPVTGEASSIQLGLD
                     FRAFPPATLDIGPVLDARDAVGSKMSALWSRGEARDYIDIDTVVESGEFSRDDVLALV
                     DQFEATPLDRRALADRFREASRHHEREFARYGVDADQRGQIVANFASWADELDPERAG
                     "
     gene            complement(17978..18322)
                     /locus_tag="PKJ61_03865"
     CDS             complement(17978..18322)
                     /locus_tag="PKJ61_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC12994.1"
                     /translation="MSATVQRGFRFEQTIPYDTPNSLDELHGPTAGTVQVRPRIDTSP
                     DPVYDLADPNQRWSLYSAVVRDGLSTEQTEILNRVLLVGLWAELNLPARCRAIWEAKF
                     PELAEVSRPRAS"
     gene            complement(18461..18880)
                     /locus_tag="PKJ61_03870"
     CDS             complement(18461..18880)
                     /locus_tag="PKJ61_03870"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HOC12995.1"
                     /translation="MILVDSGPLIAAAITDDRDHRRCVDLFATLHLNSERLLVPQTVV
                     AEVAYSIQKLGGTDQELMFLQSIADEDLVLVDLIAEDIPRVIELVNTYRDFPLGTTDA
                     SVIALAERLAITEIATLDQRHFPNVRPRHTDYLTLLP"
     gene            complement(18877..19113)
                     /locus_tag="PKJ61_03875"
     CDS             complement(18877..19113)
                     /locus_tag="PKJ61_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC12996.1"
                     /translation="MSEAREELIHLIDGLPDEKVGTLLTTARALSTPSPKSGRSWPPA
                     FFGSIEKAANGRNDNASHVDELLAESGFGQDFRE"
     gene            complement(19226..19441)
                     /locus_tag="PKJ61_03880"
     CDS             complement(19226..19441)
                     /locus_tag="PKJ61_03880"
                     /inference="COORDINATES: protein motif:HMM:TIGR01764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOC12997.1"
                     /translation="MTAERITRQPVEYLTLQEAADRYHVSVMTLRRRIRSGALPAVHA
                     GRRLIRIPAAALEQLFTPVPNSTWWTS"
     gene            complement(19967..20566)
                     /locus_tag="PKJ61_03885"
     CDS             complement(19967..20566)
                     /locus_tag="PKJ61_03885"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019044557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HOC12998.1"
                     /translation="MALKQAAARLADEFTGLYGTQTIERFLASSFDQFADRATVTRFL
                     PLLADRFARQRLYALARIEGLHSDGKPVVLFLCTHNAGRSQMALGFFQALTGDTAVTW
                     SGGSEPGTQVNPVAIQAMAERGIDISHEFPKPWTDETVRAADVVITMGCGDACPIFPG
                     KRYESWDLDDPAGQGLGTVRLIRDDLERRVRALIGSLDL"
     gene            complement(20619..21281)
                     /locus_tag="PKJ61_03890"
     CDS             complement(20619..21281)
                     /locus_tag="PKJ61_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020392397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOC12999.1"
                     /translation="MELMDRARAFAALGEESRLAIIDLLALADLAPSELGSRLGIPTN
                     LMTHHLHILEQAGLIVRRRSEGDARRVYVQRTQACNQLMGAAGGLPRPHRVAFVCSHN
                     SARSQLAESYWRTVSDIPVVSAGTRPADQVHPRAVTVGRRHGLDLTRAAPKLAEDVLT
                     GDELLIAVCDRAYEELHNRPLHWSIRDPAPADSLAAFETAYYDLTSRVDLLAASLEGD
                     PA"
     gene            21379..21804
                     /locus_tag="PKJ61_03895"
     CDS             21379..21804
                     /locus_tag="PKJ61_03895"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020385338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HOC13000.1"
                     /translation="MSNPTVLFVCIHNAGRSQMAAGYLRHLGAGRVEVLSAGSAPAGS
                     INPVAVQAMAEEGIDIAAEQPKILTTDAVKTSDVVITMGCGDTCPFYPGKRYEDWVLD
                     DPAGQGIEAVRPIRDEIRRRVENLVEELLPPHERALTRA"
     gene            21874..22299
                     /locus_tag="PKJ61_03900"
     CDS             21874..22299
                     /locus_tag="PKJ61_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13001.1"
                     /translation="MAVAERGDCDLGAVAFALEKMVDWRTAFPLLAVTTIAAPLGGPT
                     RLPADAESGTSGLRSAYRRRHEQRHRDPAVLFGLPHPHLPEARFDWTLLALTTTTSIA
                     GAQWGALFMARRVKSLTLTRVFAAALVLLALQRIFALLG"
     gene            22336..22653
                     /locus_tag="PKJ61_03905"
     CDS             22336..22653
                     /locus_tag="PKJ61_03905"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOC13002.1"
                     /translation="MVVSRSAEQLASSAGLLSAVADPIRLQLMERLAVTKACVCELQA
                     EVPIAANLLSYHLKVLREAGLIEGTKRGRWIDYELTPGALDRLRDALPGAALPERPLV
                     VVR"
     gene            22650..23702
                     /locus_tag="PKJ61_03910"
     CDS             22650..23702
                     /locus_tag="PKJ61_03910"
                     /inference="COORDINATES: protein motif:HMM:NF015715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOC13003.1"
                     /translation="MSTTTQHDTRRQAARRWLLLTAAAAAWAGLYAVNEVFWTWLYRD
                     ALALDITTGWPSMLHFFCYDTVKITLLLTGIIFVVTVLRSFMSVERTRALLGGKREGV
                     GNVLAASLGVVTPFCSCSAVPAFIGFVASGIPLGVTMSFLIASPLVNEVAIALLLGLF
                     GIGPTLLYVGAGLVVAIVAGMVIGRLRLEDQVEAFVYETRLRGEVIDSTAGLTWADRL
                     AMGREEAASILRKIWPYLLVGIGLGAVIHGWAPADFFARYAGPDNPFAVLVAVAIGVP
                     LYSNAAGILPLVDALYAKGMPMGTLLAFMMATVALSLPEMILLRRVLKPRLIATFAAV
                     TALGIVTVGYLFNALL"
     gene            23714..23950
                     /locus_tag="PKJ61_03915"
     CDS             23714..23950
                     /locus_tag="PKJ61_03915"
                     /inference="COORDINATES: protein motif:HMM:NF024590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOC13004.1"
                     /translation="MHVKILGSGCANCVNLEKATRQAVNDLGLEATFEKVTDYADIAA
                     YGIMRTPGLVVDEQVLLAGRVPTARQVKDLLAAR"
     gene            complement(23951..24733)
                     /gene="arsM"
                     /locus_tag="PKJ61_03920"
     CDS             complement(23951..24733)
                     /gene="arsM"
                     /locus_tag="PKJ61_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HOC13005.1"
                     /translation="MTDNDVVVEQVRNRYAAAARAAMGTGRRTLDILDDLDGAGSGCC
                     GGDDCGDVFGSALYDTEQADEVPTEAVTASLGCGSPTAVADLNPGDRVLDLGSGGGLD
                     VLLSARRVGPSGFAYGVDMTDEMLELARANAAKAGATNVKFLKGRIEQVPLPDGAVDV
                     VISNCVINLSVDKSAVLAEMFRVLAPGGRVGVSDVVAEDQLCVAEREERGSHVGCVAG
                     ALSRQEYVDGLTAAGFTGIEVRFTHEVAPQMHGAIVRAVKPA"
     gene            complement(24742..25134)
                     /locus_tag="PKJ61_03925"
     CDS             complement(24742..25134)
                     /locus_tag="PKJ61_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020575167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOC13006.1"
                     /translation="MSSQEVPRRLNLVSGLCCSPLLQAPLGADDALALATALKVLADP
                     ARLRLLSLIKSTEGGRATTGVLAAQVRLTQPTVTHHLGALFEAGFVERERDGRQTWYW
                     VSSDALQAIQQLLDPAPAKQLLIIGPRK"
     gene            complement(25203..26330)
                     /locus_tag="PKJ61_03930"
     CDS             complement(25203..26330)
                     /locus_tag="PKJ61_03930"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HOC13007.1"
                     /translation="MGRRGFGYIRRLPSKRYQASYTGADLQRHVAPLTFTLKSDAEAW
                     LADERRLLESGDWQPPAHRRALALRDANTETLEEYGRRWLAERRVKGEPLRPSTVLDY
                     ERALRLYITPHLGDLPVPRITKADVRNWHNNLLRGVKDRPKAKAYAVLRTIMNAAVDE
                     ELIDTSPVSIRGAGVARRKHGIEPASLEELAVIVEAMPERFRLMVLLATWCALRYGEL
                     AELRRKDVDLAHGVIKVRRAVSFVPGATIVGPPKTEAGIRDVAVPPHLHDALRHHLDE
                     FTKPGRDSLLFRSDMGRHLHESVLYRYWNTARKAAGRAELRFHDLRHTGATLAAQVGA
                     TTAELQARLGHTTADAAMIYQHAAKGRDEQLATALSRLAIG"
     gene            complement(26500..26575)
                     /locus_tag="PKJ61_03935"
     tRNA            complement(26500..26575)
                     /locus_tag="PKJ61_03935"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(26540..26542),aa:Arg,seq:cct)
     gene            27142..28434
                     /locus_tag="PKJ61_03940"
                     /pseudo
     CDS             27142..28434
                     /locus_tag="PKJ61_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015069687.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0070569 - uridylyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
     gene            28494..28874
                     /locus_tag="PKJ61_03945"
     CDS             28494..28874
                     /locus_tag="PKJ61_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13008.1"
                     /translation="MSGRWHIALGFALTGDELDLDVAADGGPGDEPNPERLVTQLEQG
                     GWSRERILEHALSRVTGDLSWPHFVPAELRTGCGAAQFHAALGATRALLDLQVLESRP
                     PSGRTRLSPDEERLLRDVPPHHGV"
     gene            complement(29013..29378)
                     /gene="mscL"
                     /locus_tag="PKJ61_03950"
     CDS             complement(29013..29378)
                     /gene="mscL"
                     /locus_tag="PKJ61_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017935786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HOC13009.1"
                     /translation="MKGFKDFLMRGNLIDLAIAFILGASFGNVVTAFTQIIMDIIGLI
                     GGNPDFSSVNIGGVNVGVFITAVVSFVIMAAVLYFGVVKPYESIKAYTAKTEAEPEAG
                     APTSEDLLTEIRDLLKAQK"
     gene            complement(29431..29982)
                     /gene="cpaB"
                     /locus_tag="PKJ61_03955"
     CDS             complement(29431..29982)
                     /gene="cpaB"
                     /locus_tag="PKJ61_03955"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HOC13010.1"
                     /translation="MFAAIAVFAALSVVAARGDTGTFAVVAVRTIAGGAVVTEADVGV
                     VRVPASVLPAEAFTEADAVVGQTVIAPVPSRRVLSPSDLLGGSSQVADGKLALPVRFE
                     DAGALGLLRVGTIIDVLGASETGSGFGVIAAKVRVVAIPQSEGGGLLGASETKIVLIE
                     VTPAQAESISAASSVATLSFALH"
     gene            complement(30101..30391)
                     /locus_tag="PKJ61_03960"
     CDS             complement(30101..30391)
                     /locus_tag="PKJ61_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004810823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOC13011.1"
                     /translation="MPTYQYRCTSCANELEAVQKFTDDPLTDCPDCDGNLRKVYNAVG
                     VVFKGSGFYATDNRTKGKTNAAKPVETSAAGSATSDAKSSAPKSSTGASTAA"
     gene            complement(30517..31119)
                     /locus_tag="PKJ61_03965"
     CDS             complement(30517..31119)
                     /locus_tag="PKJ61_03965"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HOC13012.1"
                     /translation="MTPGASEGQPRASHSALAKAALREQLRAARMARIADARAESSRT
                     SRAVTLASGAAVVAAYVSRPDEPDTALLLEWLHHSGVRVLLPVLTREPDWAWYSGQAG
                     LVAGPLGIRQPSGRPLGADALAQADVIWLPGLAGTTAGDRLGTGGGWYDRALGSARSD
                     AQLGLLLFDEEVLPDLPTEDWDRRVHFLVTPSRLVTCAAE"
     gene            31137..32417
                     /locus_tag="PKJ61_03970"
     CDS             31137..32417
                     /locus_tag="PKJ61_03970"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HOC13013.1"
                     /translation="MPLFTRAKEEAPAEALVPEPKLLPGAPDASAAAVRSIDDHRDYL
                     LANVPSLRPFGIGLLDAAGLTLCESITADIDLPTFTSATTDGWAVRAANLVGASESRP
                     VVLPVVDEIAAGGFRGAPLTPGTAVKVEQGAPIPEGADAVVPLADGVVLGEDVRFVGE
                     ATFQQNLQMLGSRISDGDALLETGVKLTPRAIGLLAEVGHDKVLARPRPRVVVLTAAR
                     ELVEPGLPLTQLHQRYDSGTVLLAASARTDGAQVFPAAIADPEPKALERSLSEQLVRA
                     DLVLLVADATDELVNLLGTVGAVDTAEVDALPGRQVFSLVGSEKTPVLVLPNEPVAAY
                     LTYLLFGRPLLQKLAGVEAYLPTEVTAPVTTDIEADADRTRLLLARHTSRGVTPIPVA
                     VPGAAELADANAVVVVPAGDNIAAHGDVSLWLLY"
     gene            32425..33054
                     /locus_tag="PKJ61_03975"
     CDS             32425..33054
                     /locus_tag="PKJ61_03975"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012923322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HOC13014.1"
                     /translation="MRVHWPVRLTHGAITLRPLRRRDEAEWARIRRRAGAWFKPWDSS
                     RPADAVEPGLTFRQLVAQFSARARRGQMLPWAVEYTPSDTKRRLFVGQVTVTGITRGS
                     ACWAQIGYWIDPKWAGRGIIPTAVAMACDYCFTTLRLHRVEIAIRPENTNSLAVVRKL
                     GFRYEGRRERYMHVDGDWRDHDMFVLTAEEVPEGVLARYERAKSVPSAG"
     gene            33162..33890
                     /locus_tag="PKJ61_03980"
     CDS             33162..33890
                     /locus_tag="PKJ61_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13015.1"
                     /translation="MTGLIFAVIAAFWLIYLIPYFLHHRNVETAEDDADAAIPFTPDV
                     TIVRSGTSLAEADPGTVPVSTPLTRRAQLRELRLLDERAAQRRRRVLMFLLVVQLIVA
                     GLVVLGVGAWWSALIPAGLIVAFLVVARFSVRTLRAGLAERARKIRECGDEETVAIVL
                     TADQVAEHEHSVELSVPIGKVGSLWDPIPITRPTYVSTPLAPRTVRTIDLSFPVAAPA
                     RMPVTADPVSPAAAIEPESQEDVG"
     gene            complement(33961..35289)
                     /locus_tag="PKJ61_03985"
     CDS             complement(33961..35289)
                     /locus_tag="PKJ61_03985"
                     /inference="COORDINATES: protein motif:HMM:NF014741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HOC13016.1"
                     /translation="MDTAEITAELQSLLDCCIAAELAAMPSAELAPWQFNEAELLAEL
                     EQARRVRDQHNARWLALLAEAEKREACLRRDSVPTSNWLVNQNSHSARSARNEVRLAM
                     KLVAAPMIADALAAGRMSTEQAAAIVNGLERLPGQLDQQQREQITQQLVGFAGEFGPS
                     GLARLVNRAVEVVAPEIAEDADRKAVERLDAQQRRDRYLTWRQDLEGAWIINGKLPTL
                     VGEQLIGVLRALASKHRKQVLLTGEPIARSQAAADALVMLVDHFQSCGVAPTYGADRP
                     RVIITIDHETLCGKLGTATLLSTDQPITAADARRLACDADILPVVMNGNSVPLDVGRE
                     KRLFTNELRQLLILRDRGCTLPGCDRPPAETEAHHRKPWWDGGQTSLDNGVLLCSFHH
                     HLVEPNPNGPPDRQWLIRLDHRGLPEFAAPLRPGQTQRIWRQHERFRGVG"
     gene            35572..36141
                     /locus_tag="PKJ61_03990"
     CDS             35572..36141
                     /locus_tag="PKJ61_03990"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOC13017.1"
                     /translation="MPSLTARPANAILFDRGVLTRVRPQDAADVIAAVAANADQLRDW
                     IPWADVPEARRAKAEGAEADWEADRAYVYALRETPLGPVIGGLGAYRRVGPGAIELGY
                     WLASEHVGRGLASAATAALASVVSALPDIERVELHIDEANARSVAVARRLGFRLARTD
                     AYEPRTAAETGRLQIWVRAVEMEPGSPSA"
BASE COUNT         5807 a        12162 c        12467 g         6240 t
ORIGIN      
        1 catcggtgtt ccagtggttc atcgcctcaa gcgcgatgat ggtgagccgc cgatcgatgg
       61 ccagctgagt gagttcggcg tcctggacgc agtgcggcca gccccagaac acctggcccg
      121 ggcgcagttc aacgatgtcg ccgggcactg gtttgggctg caggatcaca tcacactccg
      181 cgaccaactc ggcgtgactg cgtagccctg ccaccagttg gcccaactcg gcgtcgctgg
      241 ccgagaagct ctcgccgtag cccttctcga ggtacaggcg tccgctcact tcggcgggct
      301 gcttcgccag gtgcgctgga tgcagggtcg gtcggtgctc attctcgttg cgcgaccggc
      361 ccatcacgcc gatcgtcagc ccgcggtcag gccggccttc aacggtggcc gtcatttggt
      421 caggctagcc gcggacgcgc gccgacgcag cgattcagat gttcgcgacg tctgatcgat
      481 ttttttgctc aaaccattta gcaaatccac cctaggggca ctacgctgct gtcaacgcag
      541 cggcgcgacc caggaggacg acacaatgac cgtgagcttc cccgaggtga ccgatctcgg
      601 tgccattccc actggcgaca tgcctggcga caaggtgcag atcaccccga gccacgtcgg
      661 caaggcgcag gtgatcttcc ccgagctctg gacgcagctc caaccgctgc tgcagcgccc
      721 gtccggacgg gccgtggtgg cggtctgcgg tggctcgggc gtcggcaagt ccgagaccgg
      781 ttcgctgctg gcctacggtc tgaacgctca cggcatcggc gcctacgtgc tctccggcga
      841 caactacccg cgccgcatcc cggcagagaa cgatgccgag cgactgcaca ccttccggat
      901 cgccgggctc cagggccttg cggacgcagg cctggtcgac gatgacgtcc gcgccgagtt
      961 ggccgcgctg cagtccgacg gacgcgatgc cgatcccgcg caggcgtccg tctacgcctg
     1021 gttcgacacc tacttccagg ctggacggaa cgcgctggcc gactacctgg gcacgtcggc
     1081 cgaggtcgac ttcgaccaga tcagcggcat cctggctgcc ttccacaacg gcgcgaccac
     1141 gctgcacctg aagcggatgg gtcgcacccc ggacgcactc tggtacgacg acgtcgacgt
     1201 cagcgacgtc agggtgatcg ttatcgagtg gacgcatggc aacaacgacc tgctgcacgg
     1261 cgtggacatc ccagtcctgc tgaacagcac gcccgaggag accctcgcgc accgccgtgc
     1321 gcgcagccgc gacggtgccg tcgacagccc gttcaccacc ttggtgctgg gcctcgaaca
     1381 ggccaagctg cactcccagg cggctaaggc gaaaatcatc gtttcgaagg acgccgacct
     1441 gctcgatcag gccgcctacg cccgggtcat gggcgggcgg cagcagaccg cgggcccgat
     1501 gctcaacgtt tatccggaca gcatcggtgg caccctcgcc gatctggttg agttcgtcca
     1561 gcggccggag ctggctggag tgttcagttc ggcctatctg ctgccaagcg tgttcaacac
     1621 cgatctcgac cggggattct cggtgatcga ttacgaactc agcgacctgt tcgccacccg
     1681 tgccgacctt gaggcgctca ccgcggccgg catcgacttc aagttcgact tcatcctcaa
     1741 ccacgcgtcg gtgttgtcgc cgcagttcca ggacatcctc gccaagggcg cgtcctcgga
     1801 ctatgcggat ttcttcatcg actggaacaa gttctgggcc ggtcacggca cgatgaccgc
     1861 ggacggttat cttcagcccg atccggaatt gatcaaggac atgttcttcc gcaagcccgg
     1921 gctgccgatc ctgatggtcc gtttccccga cggccgtgag gtgccgtact ggaacacctt
     1981 ctaccaggag gtgcgctaca ccgctcccga cgcgcaggac ctgatgcgcg ctgctggcct
     2041 gcagtacggt cgcgccgagg tgctggcggg tcggctggcg gacgcgctcg cgtccggcaa
     2101 gcgtcccgcg gaggccgact tcagcggtta cgaaagcgtc cgggacgcgg tggtcaacca
     2161 cgtcgagagc cgccgcaagt acctgggcca gatggacctg aacatcagtt cgccgctggt
     2221 ctgggagttc tacgccgaca ccctcgacaa gctggccggc tacggcgcgc agatcgtccg
     2281 gctggacgct ttcgcctacg cgcccaagga gccgggcgcg aagaatttcc tcaatgaccc
     2341 gggcacctgg gagttgctgg gcaaggtgaa ggatctcgcg gacgctcgcg ggctgcaact
     2401 gctgccggaa atccactcca cctacgccga gggcatccac gagaccatcg ccggcctcgg
     2461 cttcatgacg tacgacttct tccttcccgg gctgctgatc gacgccttcg atcgaaggga
     2521 cgcgtccacg ctgaagcgct ggatcgggga actgttggcg aagaagatcc ggacggtgaa
     2581 catgctgggc tgccacgacg gcatcccact gctggatctg aagggcctgc tggacgagga
     2641 gcgcatcgct tccctgatcg acaccatcgt gggccgcggc ggctacgtga aggatctgca
     2701 cggcgcgaag aacatgtact accaggtcaa cgcgacatac cacagcgcgc tgggggagtc
     2761 ggacgcccga ctgttgctcg cgcgggcgat ccagtgcttc atgcccggca agccgcaggt
     2821 ctggtacctc gacctgttcg ccggcaagaa caaccacgcc gcggttgaga aggccggttc
     2881 cggcggccac aaggagatca accggacgaa cctgtcggcc gatgacgtgg ctgaagggct
     2941 cgccaagccg gtggtgcagc ggcaactgga gctgctgcgc ttccggaaca gcttcggcgc
     3001 cttcggcttc gacgcgacct gcgaggttgc cgaaaccgcg tccgacaagc tgtcgatcac
     3061 ctggcgcaag gacggactat gggctcgcct ggacgccgac ctggcgtccg agaccttcga
     3121 gatcacgacc agctgatcgc gagcgtttgg gtctcgaaag ctcaaccagc aggaagctca
     3181 atcgggcccg ggcggtggtc agcgcgttga acaggtaccg ccgccgaagt agcgcagggc
     3241 ggcggcagtg gcgatcaggc ccaggctcag ccagctgtag gcggggtcga tcgccattcg
     3301 gttcagcatc atggtcgcgg caaacacccc gcacgccggt gccagtgcgc acacgccgcc
     3361 gctgagcgcc ttcgacccgg cgaccggcag gaagctggcc agcaccacgg ccccaattgc
     3421 ggcgatcaac gccagcagtg cgagcgtccc cacctcggac ggccatcgcg ccgcgccggc
     3481 attcagcgtc cagagcagcg gcagcgcgat cagcgcggcc acggtgtggc gccgcgtcca
     3541 ggcatgccgc cagtacgccc agccagccgt gggtgccgcc gccaccgcag tctcggcacg
     3601 gggcacctcg cggaagaacc gctccggatc ggtcatcgaa caactcccat caataccctc
     3661 tggggtatcg taccgcgctg gcccaatgaa ggcgggccct cggcgagggt cagctgttct
     3721 gcgggaagcc caactgcagt ccgcggtggc tgggatcggg ccagcgccgg gtgaccgcct
     3781 tcgtccgggt gaagaagtgc acgccctcga ttccgtgggc gtgggagtcg ccgaacagtg
     3841 agttcttcca gccgccgaac gagtaatacg ccatcggcac cggcacgggc acgttgatgc
     3901 ccaccatgcc ggcctccact tccacttcga agcggcgggc cgcgccgccg tcgttggtga
     3961 agatcacggt gccgttgccg tactcgctgg cgttgatgat atccagaccc tcctggtagc
     4021 ccgacacccg cactaccgag agcaccggtc cgaagatctc gtcggtgtag atggtcatgt
     4081 cggtggccac gtggtcgaac agtgtcggcc caacgaagaa gccgtcctcc gatccggcga
     4141 acgggccgtc gcggccgtcc atgaccagcg tcgcaccggc ttcggcgccg gcggcgatat
     4201 agccggtgat ccggtcccgc gcggcctcgg tgacgatcgg gcccatgtcg cagccggtcg
     4261 agccgtcacc ggtcttcagc gtccgcatcc gttcgacgat cttcgcgacg agttcgtccg
     4321 cgatggagtc ggtggcaagc accaccgaga ccgccatgca acgctcgccg gccgaaccaa
     4381 aaccgccgct gaccgctgcg tccgcggcca ggtccaggtc ggcgtccggc agtaccagca
     4441 tgtggttctt cgcgccgccg agagcctgga cgcgcttgcc gttggcagtc ccgcgcgcgt
     4501 acacgtactt ggcgatcggc gtcgagccca cgaacgacag cgccttcact ttggggtggt
     4561 cgagcagcgc atccacggcc tccttgtcgc cgtgcaccac gttcacgacg cctgccggta
     4621 gcccggcttc gcgccacagt tcggccatcg cgttggcagc ggacgggtcc ttctcggacg
     4681 gcttgatcac cacggcgttt ccggcggcga ccgcgaccgg aacgaaccac atcggcacca
     4741 tcgccgggaa gttgaacggc gaaatcacgc ccaccacgcc gaggggttgt agcagcgagt
     4801 aggcgtccac gttggtggac acgttctcgg agtggccgcc cttgagcaac tgcggaatgc
     4861 cgcaggcgaa ctcgaccact tccaggcccc ggctaacctc gccgagcgca tcggaggtga
     4921 ccttgccgtg ctcggcggtg atcagttcgg cgatccgctg cttgttggcg ttcagcagtt
     4981 cgcggtaggc aaacagcacc ttcgtccgct tggccagcga agtggcaccc cactgtttcg
     5041 cggccgcgtc cgcctgctcg acgacctcgt ccaccaggtt cttggacgcg aggtcgaggc
     5101 tcccggtgat ctcgccggtg gccggattga agaccggcga ggtgcgctcg gctgtcccct
     5161 cccagtactc gtgcccgatc aggtgagtga ttcgcttggg tgcttgcgtg gctacgtcag
     5221 aggtcatgac ctaaatgtta gtacattagg acttagtacg gtcgggggta ttcttgctta
     5281 gtccagccac cggacggtca gctccaaagc cggtggtagg cgttgatagc gggctggccg
     5341 ccgagatggg cgtagagcac gttcgagtcg cggctgatct cgcccgagga caccaggtcg
     5401 atcagcccgg ccagcgactt cccttcgtaa accgggtcgg tgatcatcgc ctccagttcg
     5461 gcgccgagcc ggatcgcggc catcgtggaa tccaccggaa tgccgtacag gtcaccggcc
     5521 cagccttcaa gcacggtgat ctcgtcgtcg cgcacgtcgc ggccgagctc gatcagttca
     5581 gcggtgtgcc gggcgatccg ggcgacctgg tcacgggtct tcgccagcgt ggcggacgcg
     5641 tcgatgccga gcacccgccg ccggacgccg gtgagctcct ccagcgcggc gaagccggcg
     5701 atcatgcccg cgtgggtgga gccggtcacc gtgcagacga tgatcgtgtc gaagtggacg
     5761 cccagttgct gctcctgctc ggccacctcg aacgcccagt tggcgaagcc gagcccgccg
     5821 aggggatgct cggacgcgcc ggccgggatc gcgtacggtt tccctccgga cgcctccacc
     5881 tcagcgaggg cgttcttcca ggagtcccgg atgccgatgt cgaagccgga cgggtcgagc
     5941 cgggagtcgg cgcccatcat ccgcgagagc aggatgttgc ccacctgggt gttggtcggg
     6001 tcatcccagg gcacccactg ctcctgcacc agccggcact tcagcccgag gtgggcggcg
     6061 acggccgcga cctgccgggt gtggttggac tggtagccac cgatcgagac gagagtgtcg
     6121 gcaccggacg cgagcacgtc cggcacgatg tactccagct tgcgtacctt gttgccgccg
     6181 aaggccagac cgctgttcac gtcctcgcgc ttggcccaga cctgcgcgcc gcccagatgc
     6241 tggctgagcc gcttgaggtg ttggacgggg ctcggcccga aggtgagcgg gtagcgcggg
     6301 aattcctgaa gacgcattgg gttcctccgt tcggtaggtg gcggcagcct acgcccagcg
     6361 tccgttccga cggctgcaga agggaaccgt cgccgctcga tggtccgctc gttcctcgag
     6421 cgcaatcggt tagggcgcca ggccgatgga ctccaaccat tcgagctgac gggccacctg
     6481 gacggtctgg ccgccctcgt ggtcattgaa ggggtagacc tccagggact tggccccggc
     6541 gtaggcgtgg taggccgcgt acaccgtcga aggcgggcag atctggtccg cgatcgccac
     6601 cgagaacagc gccggggcgg acgctcgggc ggcgtggtgc accccgtccg cgtaggccag
     6661 cacaccgaac acctcgtcca cactggcgcg atgcactttc aggtagcggg cgatctcgac
     6721 gtatggatcg gtctcggcca catcgacggc ccgccggaag ttgcacagga agggcacgtc
     6781 gggcatcacc gcagccagtc cgtccgccag accggcgaca gcaagcgtca acccgccgcc
     6841 ctgactgatg ccggtgacca ccacccgctc cgggtcgacc tgcggcagca cgcgagccgc
     6901 gtccacagcc cgcactgcat cggtgatcaa ccggcggtag tacagcgtct cgaagctctg
     6961 cacgccgagg gtcaggtagc ccgggaaggc cggacccgag ccgtgcggat cgggcgtgtg
     7021 cccgccgctg ccccaggacg agccctgccc gcgcgagtcc atgaacagct ccgcgtaccc
     7081 ggcgttggcc caggtgatgt gctccaacgg caggccacgg ccgccgccgt agccctggta
     7141 gccgaccacc accggcagcg ggccgtcggc cccggccggc acggtcagcc acgccttgat
     7201 cggatcgccg ccgaaaccgg ggaaggtgac gtcgtagctc tcgaccagcc gcagcccggt
     7261 ctccacccgt tcgacccggg gcagcatcgg tacctcccga gccacctgca gggtttgcgt
     7321 ccagaccgaa tcgaagtcgg ccggcgcggg ctcgtcacag acgtaacgct gcagctccgc
     7381 gagtggcagg tcgatgccgg tcatcaggcg tgcaagcccc gagccagcgc cgtggccagg
     7441 ctgggcgcga gcggcagggt tgggatcaag gttgcctgcg cggcgtggta aaggtcggcc
     7501 ttgcccggcc acaccgaatc ggctggacgg ttctgcgggt cgagttggtg gtgccaggat
     7561 ccgttctcat gatcgagcac gaagcgctcg gcgtagtccc aatagcgcgc gtagtgcgcc
     7621 gaatacgtgt ggttgccggt gcgccggaac agcgccgcgg acgcgccgat ggcctccgcg
     7681 gtcacccagt gcatccggtc ccgaaccact ggacggccgg accagtcggt ggtgtagacg
     7741 aagccatccg cgccatcggc gtgccagccg tccgcgattg cgcggtcgaa cagcgcggct
     7801 gcggccggca gccactcgac cccagtcgcg ccggcggcct ccaggtgcag cagtagccgg
     7861 gcccactcca ggccgtggcc gatggtcgcg ccataaggct tgaacttgtc gtcgggacgg
     7921 tccacgttca gctcgagctg tggcgtccag tcgctgtcgt agtgctcggg cagccgccac
     7981 tcgttggctt cggcctgggc gaccgcgaac gcagccaccc ggctcgcccg gtcaagccag
     8041 acccgatcgc cggtcacgtc cgccgcggcg aggaacgcct cgacggtgtg catgttcgcg
     8101 ttgatgccac ggtagggatc gagcacggtg aagtcgcggt tccaggtgtc cactgaacgg
     8161 ccggacgcct cgtcccaaaa ccgctcctcg aagacggcca gcgcctcggc cagcaactcg
     8221 gccgcacctt cgagacccgc agccgtcgct gaagaggcgg ccagcaccac gaacgcgtgc
     8281 gtgtaggcgg ccttctcgtc cggcgtcgaa ccgtccggcg ccaccgcgga gaaccaaccg
     8341 ccggattcgg tgtcgtgcag cacgccggtc agcccggcga gcgccgctgc cgccaccgga
     8401 cggctgccgg gacgcccggc gagcgcaccg aggcagtaga cgtgcactgt gcgacaggtg
     8461 atccaggtct gcacgccctg gtcgagccag ggaacgccgg catcggtcag ccaggccgca
     8521 ccgccgctcg ggtggggcgt cctccggccg aactcgagca ggtgctggaa gtgcctggct
     8581 agccagagcc gatgggcagg caactcgatc cagttggtga tcatgcgcac ccctcttcgt
     8641 tgaggagccc tcgcgacctt gacccctttg gcacctatcc tgccagtcca agccccgccg
     8701 caacgttccc gagcccggaa gtcccgctgc cgaaccgacg acctgcggac tctgaatgcc
     8761 gagacccgcg accgagcggt gagacggagg tcagccgcan nnnnnnnnnn nnnnnnnnnn
     8821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8881 nnnnnnnnnn nnnnnnnnng cgctgcttca ccgatctgcc tcggtcgagg gggcgcgtcc
     8941 atatgctgaa gccacgtggt cgcgacacga tcgccagatt ggagaacgac ggtggcattc
     9001 ccggacgcgc gcgcggccta cgcggcgctc gccaaccggt cggcgacact tgccgacctg
     9061 cggcagatcg tcaccgactt ccctgagctg cggccgtggg tggctgccta tccgggcacg
     9121 gacgaggcgc tgctcggctg gctggcggcc ctgcaggacc cgcaggtgga cgccgaactg
     9181 gccacacgga atgcaggcgc ggcggcctgg tcagcgccgg tcacaactgg ttcgggagtt
     9241 gcgccgtcca tcttcgctcc cgcctcgccc gtgacgaacg cgcaagcggc cgcacccgcc
     9301 taccaggctc aacccggtct gcctggacga acgaccaagc gcttcggctg gccgttggtc
     9361 gtcggtgcgg tgatcgtgct gggtttggtg gccggcgggc ttagcgccgc gttcgccacc
     9421 gggatgttcg cgccaaaggc cgcgtccggc acgtccagcg ccggacccgt gcggactgcg
     9481 tccgccgtcc catccgaggc ggaaaccgcg agccaggttg ccacccccgg ggagagcggc
     9541 gccgccagcc ggtttgtctg ctgggacggg gccgaaagcg acacggccgg ggactgcatc
     9601 cttcccgaag gcaaggacga gtactggtcc ttcctgacct acgccttccc ttcgatcgct
     9661 tcgcagcctg gctgtacgga ggccgactcg accggaaagt cgacgtacgt cggggtgacc
     9721 gtgatgtggg aatgcgaact cggcgatgcg ctgatcaggt accgctactg ggaggtgccc
     9781 agcgacgcgg aggagcatta cgcaaggaag ttcaacgcca agacggtgct caagacctac
     9841 gacctgctgg ttggcggtgc gccgaccgaa ggatgggcga agaccgacaa ggacaccgtg
     9901 gacgggccgg ccggtgtgca gcgggtcgtg ttgaccatct ggctgccgga ggaccaactg
     9961 tccgtctcgg tcgagggcga cacgagcgct gcgatgtggg cagctctcga gcaggtacgc
    10021 acgcggcccg gcgggcagat gtacggccat ccggcccagg agcgagcggt ggaggctcca
    10081 atcacggtcg aggaacgttg atcacgaggg gaacactggc aacatgacca acgactaccg
    10141 cccacggcgg gccgagtcca gccgtggcgg atggccgcgg ttgggtgcgg tcggcgtgct
    10201 ggcagcgctg ttggtgcttg gcatccttgg tttcgcgcta ctgcgtccgg ttccggcgac
    10261 ggcaccgacc cccgtggcgg ggtctgcggc agcgtccgcc acgtctgctt cggtggtcgc
    10321 cagcgggacg gcggtggcgt ccgcggcggt ttcggagact cagacaccca ctccggaggt
    10381 gagccgggag gccgtcacgg tgagtagcgt ccccaacttc cgggacgtgg ccggtgtcgg
    10441 catcgaactc gccgacggcg ccacgatggc caccggtgtg gtctaccgcg cggcgaagct
    10501 cagcaccctg tcgaaggcgg acgcggccaa gctggcggac gcgggggtga gcgacatctt
    10561 cgacctgcgc acgccggacg tggcccggcg ttcaccagac gtgaagatcg agggtgcgac
    10621 gaaccgcctg gtgaacctct atgcgctcga ccgggcaccg tccccacccc ggacgaacgc
    10681 ggccgcggcg aaggatttca tgcgcggctt gtatgtcggg ttcgtggacg atgccgatca
    10741 acgcaagcgg atcgcgtcgg tgttgaagga ggtcgcgcaa gttgagcagc cggtgatcgt
    10801 gcactgctcg gaggggaagg accgcaccgg ctgggtctcg gcgatgctgc agttcgtggc
    10861 cggcgccgat cgcgagaccg tgctggagca gtacctgctc tcgaaccagg agcgggaagc
    10921 tctgatcgcg aagtcgtatg cggcggccaa acggacgtcg ggggtcgcgg ctgcggacat
    10981 cgatcgagcg ctcaagaccg tggaggcgag ctatctgaac gcgggcctgg cgcaggtgga
    11041 gaagcgatac ggcagcgtgg acggctacct gagcaagggc ctcgggctca agcaggaaac
    11101 tctggacgca ctccgcgcga agctgcgcgt cgaggcgcgc tgagctggcc aagtccgttt
    11161 agcccggatc cgggcttagg tgtggctgcg cacaccgtcc gcggtgtgct gactcacagt
    11221 caggcggccc aaggcgttgg ccgaaggcga gctagcagca gaggaactcg tcgccaggag
    11281 atccagcctg atcgcagaac tggtcgcaag cttcgtgagc ccagtcttcg agggtgtcgt
    11341 ccgagtcgcc ggtggagagg gtgtccaggc cgacgtcgta gccggtagcg gtgtgcgtgc
    11401 tgccgtccgg gcaggtgatg gtcagatcga gctgcacccg gaagccgcgg tagctgccgt
    11461 tgaagtacgt gttgttctcg gagtattcga actcgcaggt tcgctcgccg tcgtccatct
    11521 tgacggtgtt gcaggtgcag cccgcgtggg ccttgaagcc tgctggcacg aacgagccgt
    11581 ccagcccagc gcaaatcggg caggaatcgc cgttggcgac gaagacgtag gagtcggcca
    11641 tagcagtctc ctatcggtcc gatgcggccg cgagtatggc tctggcttgg gcggacgcct
    11701 ccgccgccga ccccggcgtc cccgaaagcc gacgggcggc ggcgcgcagc ctgacccggc
    11761 gggccacgtc cttggcgcag tgcttgcgga tcgtccaggc gtcattgcgc cagcccaatc
    11821 cgtcgctgac gaccgcggcg gccgggactg aagcggccgg cagccggtag agccgccgga
    11881 cgaacacccc gtcctcgaag tcgaggaact cggacgcgac ccggtagtcg ccgcgggcgt
    11941 agcgtccgga gatcaccagc aggtcatcgg tctcgggcgg agtcgactcg acccgggtgg
    12001 tgatcacgct gctgcccagc ggcggcagcg tgtcgccgcg gcgttggctg tacctgcgcg
    12061 gtgcgcgggg ccgtccgccg agcaccaggg tcaccgccac cagcgcgccg gtgcggcgca
    12121 ccggcaccgc ggtcagcagt ttgtgcccca gctgcggatg cgtcgccgcg accgccaccc
    12181 ggttgtcgag cggtagcccg atcacccgga agcggccgcg ggcgtcggtc actgcgtccc
    12241 ggccggcaac ggtgacccgt gctccctcca ccggcgtctc gtcgcgcaac cggacgttgc
    12301 cgttgaccac cccggtgtgc cgcagccgga cggagccctt ggccagcgcc agcagttgct
    12361 cgtcgtcgcg ttgggtcagg atcggacgga gccgtgccag atcccagtcg gagaactcgt
    12421 cgataccgct gacgccggca gcctgcaggg cagcggcctc ggcgacggtg atcgtgtcca
    12481 gctgactgag ttcggcgatc gcctgccagc cgcggatctc ggtcagtgtg atctgtggga
    12541 gccggcgaac cagggcaccc cgctccgtcc gcaacaggtc gtcgagggtg aaaagggtga
    12601 ccgactccag cgcgcttgcc tgggtcgccg tgatggtggg gatgtcgctg atcagggcca
    12661 tcggtgcctc cgcgattgag ctgatctcac cattgcagcg caccgcaacg atggcatcgg
    12721 cgtacttctc gcactggcga aatccagtgg acgcgagtag tgcgctcacc tttcagtcga
    12781 tgagcccgcg ctctctagcg atgacgattg cagcggtgcg cgagggtgcg ccgagtttgg
    12841 cgtagctgtg ggcgaggtgg gttttcacgg tggccaccgt gacgaacagc tcggcggcga
    12901 tctggtggtt ggacagcccg ccggccgccc gttgcaggac ttccagttcc cgctgggtga
    12961 gggtcgtggc ggggtcgagg acgcgttgca gcaggcgctg ctggacggcc ggcgaaagcg
    13021 cgggctgacc gacggcggcg gcccggacgg ctcgttccaa ctcgtcggtg ggggtgtcct
    13081 tgagcaggta gccgcgcgcg cccgcgtcca gtgcggcgag gatgtccgcg tcggtggcgt
    13141 aggtggtgag gatgagaacg gcggggcctc ccgatgccga gatgcggcgc gtggcttcgg
    13201 ccccggtgag tcgccccttg ccgaactgga ggtcgagcag cacgaggtcg cagacgttgt
    13261 cgtccaacca ggtcaacagc gcctcggcgc tgccgtgttc ggccacgacg gtgacgtcat
    13321 ccatgccctg caacaggctc gacaggccgc tgcgcacgat cgggtggtca tcggccatcg
    13381 caatccggat catgcggact ccgaccccaa cggcagactg atcgcgatcg cggtcccttc
    13441 acctggctcc gactccactc ctacggtgcc accgagttcg cggacgcggg cgcggagcgc
    13501 cggcagcccg aagccgccga cagcctcttc tccgtccgga ttgaaacccc ggccgtcgtc
    13561 ggtgatgtcg aggagcacgc gatcaggctc ccaggtaagg ctgaccgagg cgttcgccgc
    13621 tgcagcgtgc tggacgacgt tggcgagcgc ggactgagtc attcgcagga tctccgtcga
    13681 tagttccgcc gacaggacgg gttcggttcc catggatcgg accgcgacct tcgtccgtcc
    13741 gccgctgtcg gcttgcgcgc gctcagccgc gtgctgaatg gcgcccatgg tggcgtcctg
    13801 ccgatgtccc ggctcgcctg cgatgaaccg gcgggcctgg gcgagccctt ccgctgtcgc
    13861 ctgccgcgcg agcgcgacgg cgggggctgc cggactgtca gtgcccatga gggccgccgt
    13921 ccggcgaagg tgcaggtcga tcgcagcgaa gtcctgcgcg agggtgtcgt ggatgtcccg
    13981 cgcgaggttc gctcgctcct cggcccgcaa acgttcacgt tcggcgtcca gcaaccgctg
    14041 ctgggcgtct tccagctcgt gcagggcgcg ttggcgctgg acaaccagtc gcgccaggga
    14101 ttccagaccg accacggcag cgaccgcaaa gaaggcgccg atcaccggac cgaggacgaa
    14161 gccgagcgcg acctgacccc gcaccacggc gccgagcacc actgcggcca ccgtcgtcag
    14221 cgtgaccgca accacgccat gacgcggccc gagcaggtgc agttgcagca gcatcaccgg
    14281 aaaggcgagc cacatgccgg attcgctggt gagtagcacg aggcagtagg ccgccagcag
    14341 cccagaagcg gccgcaccgc ccggccacca gccgccgcgc gcctccatcg gttcgccgag
    14401 cacgcgcacg ctgagccgga ccacgagcca gccgaacgcg aacaccgcgc cggcggccca
    14461 caccgtccat gcagccccgg acggggacgc gacggcgacg acgaggagca gcgcggccag
    14521 caggtcgagg ccgaccaaga gatgcgcagt cacccgctcc gaacccagct ccacccggga
    14581 actgtagccc gccctcgcgc gtccgccatc agccgaaaga tgcaggcatt cctcagccga
    14641 acgagccgcg aataccgtcc gcgggcgtga tgtccgtgcc gcggcgaggc gggagcatcg
    14701 attccaaccg aaacctcttg gaggaaccta tgcgtcggat ctacctggcc gccatcgtct
    14761 acgccgctct cggcctgctg ggcggactcg gctaccgcga ggtcacccat gcccgtgact
    14821 tcaccggcta cacccagctc gcagtgctac acactcacct gctcgccctc ggcatgctct
    14881 tcatgttgat cgtgctcgct ctcgatgcca ctttggggat cagcggcagc cgatcattct
    14941 cgtggttctt cacgacctac aacgccgggc tggtgatcac cgccggagcc ctcacctggc
    15001 acggactgct tcaggtcttc ggccaggcgg ccggcgctgc gatcgccggc atcgcgggcc
    15061 tgggccacat cctgctgacc atcggcatcg gctgcctgct ggtggccatc aacaagccgc
    15121 tgcgtgcacg cctcgccgac gcggcgaacg tctgaccatg cttgccctcg ccatcatcct
    15181 gatcagcctc gcgttggcgt tctacaccct cggggtgtgg gccgaacgtc gcaccggcga
    15241 gctccgctgg tggcatgtgg ccgcgttcgc agccggtctg accgcggacg tcagcggcac
    15301 cgtcgtgatg accatgatcg ctaacggcgg cggtgccacc ggggtcgagc agagccccgt
    15361 cctcgcccag ttgatggcgg ccaccggtct ggtcgcgctt ctgctgatgg cgctccacct
    15421 tggctgggcc gtagtcacga tgattcgcga tcgcttcgac gagaagcggg tcttccatcg
    15481 cttcagcatc gtggtttggg tgatctggct gattccgtac ttcaccggga tggccgctgc
    15541 gatggcctaa atgattcaca gattcggtta gtggagcttg tcgagacctg ttctgaccgt
    15601 ggatccaccc cggcgagatg cttcccagcg cccatcaact cctgaacggg attgggtcgc
    15661 agttgtcgtt gtgcgtaccg cagtagctct gcagccattc ccagaacggc ttcggctcgc
    15721 cgtcgaacgc gtacagggcc cgcggcgtcc ctcctgcctg ccgggccgtg gtgtcgggcg
    15781 gccagcatcg cggcactacc gcacgaggtg ctggcgttcg gtatcaactg ccgtaggtcc
    15841 ttcaggtcga cataggtcgt gtctccggag accttcgcgc tgatcagtgc gtccgcgccg
    15901 cgccccacac cttcgagcag tccacttcgg gatcgagcac ggtgttgccg aagtacacca
    15961 ccaccattcg ggtcggggcg tctgcggacg tcgatgaggt cggagtggtg gccgccggtg
    16021 ttgtgccggt cggcactggg ctcgtcgcgg tgggcgtggt cggagtgcag gcggcgggga
    16081 tgaacgggcc atgcttgtag cgtgtatcgc tacgcaatac actcggctgg tgatcgtcag
    16141 tttccggcac aaagggctgg agcagctgta ccgcaccggc tcgaagaagg gcgtgcagac
    16201 cgcgcacgtg cccaagctgc tgcgcattct gtctctgctg gacgtcgcgc aagccgccga
    16261 cgacctgaac atcccgtcct ttcgaactca cccgctgaag ggagagctgg ccggccactg
    16321 gtcgatctgg gtcaacggga actggcgggt cacattcagg ttcgtcgaca gcgatgtcga
    16381 actggtcgac tatcaggact accactgaag gaggtgaaca ccatggtgat gcagaacccg
    16441 gtccatcccg gcgagatcct gcgcgaggac gtcctggccg atctcggcct cagcgtcggc
    16501 gaagccgcgt cgcggctggg gatctcgcga gtcacgctga gccgcgtgct gcacggccat
    16561 gcccggatca gccccaatct tgctgtccgg ctggaggtgg ctggtgtcgg cacggcccgc
    16621 gcctggctgg cgatgcagtc cgcgcacgac ctcgccgcag agcgcgccgc cggactccca
    16681 acggtcaccc ggctcgaccc cgtcgcctga ccctcaccga ggtcccagcg acatcaccga
    16741 caatgcacat ggtgtgtgtc tcgatgggtg ttcgttccat ccaccgatag cgcgccgtgg
    16801 caacggggtg gttccggcct cagggtcggc cgtcgcccat acgggcgttt ccggcagatg
    16861 agtggagctt cggtggctga cgccagagcg tggagcgaag gctcgagctg caagcgttgg
    16921 cgcacatcct ccaccaccaa gcccgtcttg gcccaaccgt ggtggcgccg tcggtgtcgc
    16981 aggcttcaac ccgaactgcc gcgtgatcac caattgcggc tctcctgcgg tcagggtcca
    17041 tccccaacgg gcccggcgtg caacgtacgt gcaacatgat ccggttcgag agccgatgtg
    17101 tgtctgctcc tgtgcctctg ggatcactcg ccgggccgcg catttgtgct tgtcagaagg
    17161 acagatcacg accagcgtgg ttggcgaccc cagcaagatt cgaacttgcg acacaaggtt
    17221 taggaaggcc gtcgcaacag cgccttggct aggtcttatt gcacgccgag cagccatcgt
    17281 gcaacatgcg tgcaacatgt cgatcggggc gaaagagggt ttagcctgct cgctcggggt
    17341 cgagctcgtc ggcccagctg gcgaagttgg cgacgatctg gccgcgctgg tcggcgtcca
    17401 caccgtagcg ggcgaactcc cgctcatgat gccggctggc ctcccggaac cggtcagcga
    17461 gggctcgtcg gtccaggggt gtcgcctcga actggtcaac gagggccaac acgtcgtctc
    17521 ggctgaactc gcccgactcg accacggtgt cgatgtcgat gtagtcgcgg gcctcgccgc
    17581 gcgaccagag cgccgacatc ttcgacccca ccgcgtcgcg ggcgtccagg acgggaccga
    17641 tgtcgagcgt ggcgggcggg aaggctctga agtcgaggcc cagctggatg ctgctggcct
    17701 cgccggtgac cgggtccgtg acgtcgaact cggcaaaggt ggctcggaga cgtccgtcct
    17761 cgacccggag accggccgcc acgtatgcct cgcgaagccg atcgacggct gtggcgaact
    17821 gctcggggtc ggagcggttg gtgaagaggt cgacatcctc cgacggccgg tcgcccatgc
    17881 cgttcgcgga gatggcgtag ccgccggcca ggacgaaccc gaactcgccg atcgctgcca
    17941 ggccgatctc ggcgagccgg cggtggaagg gcagcattca ggacgccctg ggtcgtgaca
    18001 cctcagccag ctcggggaac ttggcttccc agatcgcccg gcaccgcgca gggaggttca
    18061 actcggccca caggccgacc agcaacaccc ggttcaggat ctccgtctgc tcggtggaca
    18121 gcccgtcgcg gaccacggcg ctgtacaaac tccagcgctg gttggggtca gccaggtcat
    18181 aaaccgggtc ggggctggtg tcaatgcgcg gtcgcacttg gacggtccca gcggtcggac
    18241 cgtgcaactc gtccaaggag ttgggcgtgt cgtatgggat cgtctgctcg aaccggaatc
    18301 cgcgctgaac tgtggcactc atctggtcga gcctccttcc cggtgcgccg ttgtcgatga
    18361 caaggctacc gagcgacgcc gacgcagcgc aggagggttc gctgagatgg cctcgcccca
    18421 caaccgcgaa gcatgcgttt cggccgcccg cgtgggtgat tcacgggagc agcgtcaagt
    18481 agtcggtgtg gcgtggtcgc acgttgggga agtgcctttg gtcgagcgtg gcgatctcgg
    18541 tgatggcgag gcgttcggcg agtgcgatca cggacgcgtc cgtggtgccg agcgggaagt
    18601 cccggtaggt gttcaccagt tcgatgactc gcgggatgtc ctcggcgatg aggtcgacga
    18661 ggacgaggtc ttcgtccgca atcgactgca ggaacatcag ctcttgatcg gttccgccga
    18721 gcttctggat gctgtaggcg acttctgcga cgaccgtctg cgggacgaga aggcgttcgg
    18781 agttcaggtg gagggtggcg aacaggtcga cgcagcgacg gtgatcgcgg tcgtcggtga
    18841 tggcggcggc gatgagcgga ccggagtcca ccaggatcat tcgcggaagt cctgaccgaa
    18901 cccggactcg gccagcaact catcaacgtg ggaggcgttg tcatttcggc cgttcgcggc
    18961 cttctcgatg ctgccgaaga acgccggcgg ccaggatcgg ccactctttg ggctcggcgt
    19021 gctgagagcc cgcgcagtgg tgagcagtgt gcccaccttt tcgtctggga gcccatcgat
    19081 gaggtggatg agttcctctc gcgcctcgct catgtgccga gtctagttct tggcaggacg
    19141 gcagcgctga acgtcagcga gtgccccaga gacgaggctc tgactgctac gagggtgacc
    19201 gatcggttct cggccgaaca ggggatcacg acgtccacca cgtcgagttc ggcactgggg
    19261 tgaacagctg ctccagggcg gccgcgggga ttctgatcag gcgtcgtccg gcgtggacgg
    19321 cgggcagggc gccggagcgg atgcgccgcc gcagggtcat gacgctgacg tggtagcggt
    19381 cggcggcctc ctgcagcgtg aggtactcga cgggctggcg ggtgatgcgc tcggctgtca
    19441 tctcgttctc ctgtctcttg cgcgtcagat gctgatgcct cggtcgcggt cgtggctgtg
    19501 gtgatagctg ggttcgtctt cgggtcggcg cgtggctggg cgtgcctcgg tgatcggtgg
    19561 ccagccgcgg ggcacgcgcg tgtgtttgct ctggtggcgt tcgaggcgga aggcgagcgc
    19621 gccgcacatt gtgtgtctgt cccggagtgt gtgttcggtt ctacgcgtcg tgagctgcgg
    19681 attggggcgg atcggcggtg tcgctggcgt ttgagagctg aggtggtcgt cagggatgcc
    19741 ttgattggct gtgctcgcgt tgggcgagga agcccgctgg tcaacctcgg cgcgattccc
    19801 cgcgttctca ccaaatccga cggcggctcg gtgcggatga tcttggtggc gacctcatgc
    19861 gacaggaggc tgcagagcgt ccacagttcc ttgcctgggc gggagtcctg cagcagtgca
    19921 gagagtcgaa atcgagggtc gtccaggtcg ccagtccgat gcttggtcaa aggtcgagcg
    19981 agccaatgag cgcgcggacg cggcgttcca ggtcatcgcg gatgaggcgg acggtgccca
    20041 ggccctggcc ggcggggtcg tcgaggtccc agctttcgta ccgcttgcca gggaagatgg
    20101 ggcaggcgtc gccgcagccc atggtgatga cgacgtcggc cgcgcggacg gtctcgtcgg
    20161 tccatggttt ggggaactcg tgcgagatgt cgatgccgcg ttcggccatg gcctggatgg
    20221 cgacggggtt gacctgggtg cctggctctg agccgccaga ccaggtgacg gcggtgtcgc
    20281 cggtaagggc ctggaagaag ccgagtgcca tctgggagcg gccggcgttg tgagtgcaca
    20341 ggaacagcac aaccggcttg ccgtcggagt ggagtccctc tatacgggct aaggcgtaga
    20401 gacgttgacg ggcgaaccga tcggcgagca ggggcaggaa cctggtgacc gtcgcgcgat
    20461 cggcgaactg gtcgaacgag ctggccagga agcgttcgat ggtttgggtg ccgtacaggc
    20521 cggtgaactc gtcggcgagt cgtgccgcgg cctgcttgag cgccaacttt cgtcggcgcg
    20581 aaggctttcc caggatcgtt ccgaaacggg gtgagtggtc atgccgggtc tccttcgagg
    20641 ctggccgcga gaaggtcgac gcggctggtg aggtcgtagt aggcagtctc gaacgcggcc
    20701 aagctgtcgg cgggggccgg gtcgcggatc gaccagtgca gcggtcggtt gtgaagttct
    20761 tcgtaagcgc ggtcacacac ggcgatcagg agttcgtctc cagtgagaac gtcctcggcc
    20821 agtttggggg cggcacgagt caggtcgagg ccgtgcctgc ggccgacggt gactgcgcgc
    20881 gggtgcacct gatcagcggg gcgggtgccg gcggagacga ccggtatgtc gctgacggtg
    20941 cgccagtacg actcggcaag ctgtgaccgg gccgagttgt gggagcagac gaacgcgacc
    21001 cggtgcggcc gcggcaggcc gccggcggct cccatgagct ggttgcaggc ctgcgtgcgc
    21061 tggacgtaga cgcgccgagc gtctccctcg gaacggcgac ggacaatcag cccggcttgt
    21121 tcgaggatgt gcaggtggtg ggtcatcagg ttggtcggaa tcccgagccg tgagccgagt
    21181 tcactggggg ccaggtcggc caaggcaagc aggtcgatga tggccaagcg cgactcctcg
    21241 cccagtgcgg cgaaggcgcg ggcccggtcc atcagctcca tctcgatccc tccacacttg
    21301 ttgcctcaac actaactgag ctattttgga agtcaagcct cgcttctggg gcttccttca
    21361 gccaaggagt cgccaagaat gagcaatcca acggtcctgt tcgtgtgcat ccacaatgct
    21421 ggacgctcac agatggccgc cggctacctg cgtcacctgg gagcgggacg ggtcgaggtg
    21481 ctctcggccg ggtcggcgcc agcgggctcg atcaacccgg ttgcagtgca ggcaatggcc
    21541 gaggagggca tcgacatcgc cgccgagcaa ccaaagatcc tcactaccga cgcggtgaag
    21601 acctccgacg tcgtgatcac gatgggctgc ggcgacacct gcccgttcta tcccggcaag
    21661 cgctacgagg attgggttct tgacgaccca gccggccagg gcatcgaggc cgtccgcccc
    21721 atccgcgacg agatccgccg tcgagtggag aatctggtgg aagagcttct gccgccgcat
    21781 gagcgagcgc tgacgcgcgc ttgaaagccc ggcccgcgcc cctccggtgc tggcgatgtt
    21841 cgggctggag ctcaagcaca agatcatgcc gctgtggctg tggctgaacg gggtgactgc
    21901 gatctcgggg ccgtcgcctt tgctcttgag aagatggtcg attggcgcac cgctttcccg
    21961 ttgctggccg tgaccacgat cgcggcgccg ctagggggtc cgaccaggct tcctgctgat
    22021 gccgagtctg gtacttctgg gctacgcagc gcgtaccgcc gccgccacga acagcgccat
    22081 cgtgaccctg ccgtcctttt cggccttcct cacccacacc tccccgaggc acgcttcgat
    22141 tggacgctgc tggccctgac cacgaccact tcgatcgccg gcgcccaatg gggcgccctg
    22201 ttcatggcca gacgggtcaa gagcctcacc ctcacccgag tcttcgccgc cgcgctcgtc
    22261 ctgctcgcgc tgcaacgaat cttcgccctg ctcgggtgag ttgacagctg tcatccaggc
    22321 gggaatgatt caaacatggt tgtttcaaga tcagctgaac agctagcgtc ctcggctggc
    22381 ctgctctccg ccgtggccga cccgatcagg ctccagctga tggagcggct cgccgtgacc
    22441 aaggcgtgcg tgtgcgaact ccaggctgag gtcccgatcg cggcgaacct gttgagctat
    22501 cacctgaagg tgctgcgcga ggcgggcctg atcgagggca ccaagcgtgg ccgctggatc
    22561 gactacgaac tgacgccagg cgcattggac cgtctccgcg acgcgctccc tggggccgcc
    22621 cttcccgagc ggcccctggt ggtcgtgcgg tgagcactac gacccagcac gacacccgca
    22681 ggcaggcagc ccgccggtgg ctgctcctga ctgccgctgc cgctgcctgg gccgggcttt
    22741 acgcggtgaa cgaggtcttc tggacctggc tgtacaggga cgcgctggcg ctcgacatca
    22801 ccaccggctg gccgtcgatg ctgcacttct tctgctacga caccgtgaag atcactctgc
    22861 tgctgaccgg gatcatcttc gtggtcaccg tactgcgcag cttcatgagc gtcgaacgca
    22921 cccgggccct tctaggcggc aaacgtgaag gggtgggcaa cgtgctggcc gcctcgctcg
    22981 gggtagtaac cccgttctgc tcgtgcagcg ccgtgccggc gttcatcggt ttcgtcgcct
    23041 ccggcattcc cttgggggtg acgatgagct tcctgatcgc cagcccgctg gtcaacgagg
    23101 tcgcgatcgc cctgctgctc ggcctgttcg gcatcggccc caccctgctg tacgtcggcg
    23161 cggggctggt ggtcgcaatc gtggccggca tggtgatcgg gcgactccgg ctggaggatc
    23221 aggtcgaggc cttcgtttac gaaacccggc tccgcggcga ggtcatcgat tccaccgccg
    23281 gtctgacctg ggccgacagg ctcgcgatgg gacgcgagga agcggcctcg atcctgcgca
    23341 agatctggcc gtacctgcta gtcggcatcg ggctcggcgc ggtcatccac ggctgggcac
    23401 cggcggactt cttcgcccgt tacgccggtc cggacaatcc attcgctgtg ctggtcgccg
    23461 tggcgatcgg cgtgccgctg tactcaaatg ccgccgggat cctgccgctc gtcgacgcgc
    23521 tgtacgccaa gggcatgccg atgggcaccc tgctcgcctt catgatggcc accgtcgcgc
    23581 tcagcctgcc cgagatgatc ctgctccgcc gcgtcctaaa gccccgcctg atcgccacct
    23641 tcgccgccgt taccgccctc ggcatcgtca ccgtcggcta cctcttcaac gccctcctct
    23701 gaaaggacaa cacatgcacg tcaaaatcct cggttccggc tgcgccaact gcgtcaacct
    23761 cgagaaggcc acccggcagg ccgtcaacga tctcggcctg gaggccacct tcgagaaggt
    23821 caccgactac gccgacatcg ccgcctacgg gatcatgcgc accccgggcc tggtggtgga
    23881 cgagcaggtc ctgctggccg gacgcgtccc caccgcccgc caggtcaagg acttgctcgc
    23941 cgcccgctga tcaggccggc ttgaccgccc gcacgatcgc gccgtgcatc tgcggggcca
    24001 cctcgtgggt gaaccgcacc tcgatgccgg tgaacccggc agcggtgagc ccgtcgacgt
    24061 actcctggcg ggacagggcg ccggcgacgc agccgacgtg gctgccccgc tcctcgcgct
    24121 cggcaacgca gagctggtcc tcggcgacaa catcggacac accaacccga ccgcccgggg
    24181 cgaggacgcg gaacatctcc gccaacacgg ccgacttgtc cacggacagg ttgatcacgc
    24241 agttggagat caccacatcc accgcgccgt ccggcagcgg aacctgctcg atacgtccct
    24301 tcaggaactt cacgttggtc gcaccggcct tcgccgcatt cgctcgtgcc agctcgagca
    24361 tctcgtcggt catgtcgact ccgtaggcga agcccgatgg cccgacccgt cttgcggaca
    24421 gcagtacgtc caggccgcca cccgagccga ggtcgagcac ccggtcgcct gggttgaggt
    24481 ccgcgaccgc ggtcgggctc ccacacccca ggctcgccgt caccgcctcc gtgggtacct
    24541 cgtcagcctg ctcggtgtcg tacagcgcgg atccgaacac gtcgccgcag tcatccccgc
    24601 cgcagcagcc actgccggcg ccgtcgaggt cgtccaggat atcgagcgtc cgtcgcccgg
    24661 tgcccatagc tgcgcgggca gcggccgcgt accggttccg gacctgctcc accaccacat
    24721 cgttgtcggt catcgctgca ctcacttcct tggtccgatg atcaggagtt gcttggccgg
    24781 ggccgggtcg aggagctgct ggatcgcttg cagcgcgtcg gaactgaccc agtaccaggt
    24841 ctggcggccg tcgcgctccc gctccacgaa ccccgcctcg aacagcgcgc ccaagtggtg
    24901 ggtcacggtc ggctgggtta gcctcacctg cgcggccagc acgccggtcg tcgcgcggcc
    24961 gccctccgtc gacttgatca ggctcaacag ccgcagccgg gcaggatcgg ccaacacctt
    25021 cagcgcggtc gcgagagcga gcgcgtcatc ggcccccagc ggggcctgga gtagcggtga
    25081 gcagcacagg ccgctgacga ggttgagccg gcgcggcacc tcttgagaac tcatagacct
    25141 aaatctatca tatcgactac catcaaccaa tcggctactt gagccatgag cgcgcgtctc
    25201 ggtcatccga tcgcgagtct cgagagcgcc gtcgcgagtt gctcgtcgcg ccccttggcc
    25261 gcgtgttggt agatcattgc ggcgtcagct gtggtgtggc cgaggcgggc ttgaagctcg
    25321 gccgtggtcg cgccgacctg agcggcgagg gtggcgcccg tgtggcggag atcgtgaaag
    25381 cgaagctcgg cccgcccggc ggccttacgg gcggtgttcc aataccgata gagcactgac
    25441 tcgtgcaaat ggcgccccat gtcgctgcgg aacaacaggc tgtcgcgtcc cggcttggtg
    25501 aactcatcca agtggtgccg aagcgcatcg tgtaggtgcg gtgggacggc cacgtctcgg
    25561 atgccggcct cggtcttcgg gggtccgacg atggtcgcgc cgggcacgaa cgacacggcg
    25621 cggcggacct tgatcacgcc gtgggcgagg tccacgtctt ttcgtcggag ttcggccagt
    25681 tcgccgtaac gcaaggcgca ccaggttgcc agtagcacca tgagccggaa ccgctcgggc
    25741 atcgcctcga cgatgaccgc cagctcctcc aatgaggccg gttcgatgcc gtgcttgcgt
    25801 cgcgccaccc cggcgccgcg gatgctgacc gggctggtgt cgatgagttc ctcgtcgaca
    25861 gcggcgttca tgatcgtccg cagcacggcg tacgccttgg ccttcggccg gtccttcaca
    25921 ccgcgcagca ggttgttgtg ccagttgcgg acgtcggcct tcgtgatccg ggggactggc
    25981 aggtcaccca ggtgcggcgt gatgtagagc cgcagcgcac gttcgtagtc gagcacagtg
    26041 cttggccgca gcggttcccc cttgacgcgg cgctcggcca gccaacgcct cccgtactcc
    26101 tccagcgttt cggtgtttgc gtcacggagg gccagagcac gccggtgcgc tggcggctgc
    26161 caatcacccg attcgagcaa gcgccgctcg tcggcgagcc atgcctcggc gtcactcttg
    26221 agggtgaacg tcaacggcgc cacatgccgc tgcaggtctg ccccggtgta gctggcctgg
    26281 tagcgcttgg agggcagccg cctgatgtag ccgaagcctc gtctgcccat ggtcgcctcc
    26341 tgagcgttcc gcacgtcctc ccgctatcgc gcgacgtgcg gtccacgtgc gataagtccc
    26401 gctacgagtg ctcattcgtg tccccgagag aacacttcag tgcgtctggg aggctgcgcg
    26461 tttctgcttg tcagaagcat atctcacgct tacgacgtct ggcgacccca gcaagattcg
    26521 aacttgcgac acaaggttta ggaaacctct gctctatccc ctgagctatg gggcctcgcc
    26581 ccaactggag ctcaaccagc ctaccgcttg ggggcgtgcg tccggacgcg gcggtgcggc
    26641 aacccgccta ccggtccgcg tccgaccact cgtgcagccg gtctccgtag tacgcctggt
    26701 gtgcgccgtc atggtgctcg gccatgcggc actccagggt cggaccgtcc gatcgctgtg
    26761 accactgcga gccgcagctc cccggcgact tcggcaccgc atggctcatc ccggtttccc
    26821 tcacctaacg cagacgtcgc ccgcaccgat tcatgacgcc gtactctgcc acaaagctcg
    26881 gcgaatacct acgactacgg cttttagtgc gccttggggc aaacactcct gtggaaaacc
    26941 tgtgaaatgg tgggtgggct ttgttttggg gttcccagtg ggctaaagtc tcactcagag
    27001 tgacgcactc gatgttgtct cctgggggtg ggtagacggc ttcaagccct tcgtggtgcg
    27061 tcactctctt actgtcttcg ggccgttccg cgaacggagt tgtggctcgg ccaaaggcgc
    27121 cgcaccgtta cgatcggccc catgagcgct accgccttgg agcagtgcct gcagaagatg
    27181 cgggacgccg acatcagcca ggccgcgatc gacaacttcc gccactactg gcaactgtgc
    27241 gcggacgggg cgaccggcct gatccgcgag gacaccatca cgccgctgct ggatccgccc
    27301 cagctcgccg aggttgagat ctcggacgcc gaccagcgtg acgccttgag caagaccgtc
    27361 atcatcaagc tcaacggtgg cctcggtacc tcaatggggc tggatcgcgc caagaccctg
    27421 ctcaccgtcc gcgacggcaa gaacttcctc gacctgatcg tcggccaggt gaagtacgcc
    27481 cgcgagcact tcggcgtccg cctgccgctc gtcttgatga acagcttccg cacccgcgac
    27541 gacaccctcg cgcacctcgc cacacacccc gacattgcgg tggatgggtt gccgctcgac
    27601 ttcctgcaga acgccgagcc gaagatcctc gccagcgacc tcagcccggt ctcgtggccg
    27661 gccgatccca gtcttgagtg gtgcccgccc ggccacggcg acctgtatcc cgcgctctgg
    27721 ggcaccggcc tgctggacgc cctggtagag gccgggttcc gctacgcgtc cgtcgccaac
    27781 ggcgacaacc ttggcgcagc cccggacgcc cggttggccg gctggttcgc cgcctccgga
    27841 gcgccctatg cggccgaggt ttgcccgcgc accatcaacg atcgcaaggg cgggcacctc
    27901 gcgatccgcc gcgccgacgg ccagctgatt ctgcgcgaca ccgctcagac cgcgtccgaa
    27961 gagatggact acttcaccga cgagcatcgc cacccgtact tccacgccaa caacctgtgg
    28021 ctcgacctcg tgcagttgcg cgacatcctc gccgagcgca agggcgtgct cggcctgccg
    28081 ctcattcgca acgagaagac cgtcgatccc gccgatccga cctcgccgaa ggtgctccag
    28141 ttggagagcg caatgggcgc ggccatcgag gtgttccacg gagccatggc catcgcggtc
    28201 ggacgggacc ggttcctgcc cgtcaagacc accaacgaac tgctgctgct gcgctcggac
    28261 gtgttcgacc tcggtgtgga cggacggctg gtgagccagg tcgagcgcat cccggagatc
    28321 gatctggaca gccactacaa gctcgtcgac gacttcgacg aattggtgca ggtggtgcca
    28381 tcgctgcgca acgccgagtc cctgcgggtc gcgggggagt ggcatttcga tgcaccttcc
    28441 tccgtggtcg ggcaggtgca gctcgacgac ccggggacgc cggcgaccta ccggtgagcg
    28501 gacgctggca catcgctctc gggtttgcgc tgaccgggga cgagctggac ctcgatgtgg
    28561 ctgctgatgg cggaccgggt gatgagccga atcctgagcg gctggtcact caactcgagc
    28621 aaggcggctg gtcgcgggag cgcattctag agcatgcact gagccgggta acaggcgacc
    28681 tcagttggcc gcacttcgtg cccgcggaac tacggacggg ctgtggcgcg gcgcagttcc
    28741 atgccgcgct cggggccacc cgggcactgc tcgacctaca ggtgctcgaa agccgtcccc
    28801 cgtccggacg gactcgactg agtccggacg aggaacggct tctgcgggac gtgccgcccc
    28861 atcacggtgt gtgagcctgg ccgctagcag tcgggccgag ctccccgatc acgcccagag
    28921 atcccggcct gcgccgggat gactgtgtgg tgggatcccg gcctacgccg ggatgactgt
    28981 gtaggccggg atgacggggg tggcggccgg tgctacttct gtgccttcag caggtcgcgg
    29041 atctcggtga gcagatcctc ggacgtcggt gcgccggcct caggctcagc ctcggtcttc
    29101 gcggtgtacg ccttgatcga ctcgtacggc ttgacgaccc cgaagtacag caccgcggcc
    29161 atgatcacga acgagaccac cgcggtgatg aacacgccca cgttcacgcc gccgatgttc
    29221 accgagctga agtcggggtt gccgccgatc agcccgatga tgtccatgat gatctgggtg
    29281 aacgcggtga cgacatttcc gaacgacgcg cccaggatga acgcgatcgc caggtcgatg
    29341 aggtttcccc gcatcaggaa atccttgaag cccttcataa ttctccctcc attggctagc
    29401 gcgggtgatg ttgcccggtt tctgcgctga tcagtgcaag gcgaagctca atgttgcgac
    29461 gctggaggcg gccgagatcg attcagcctg cgctggcgtg acttcgatca agacgatctt
    29521 ggtttcgctt gccccgagca gcccgccacc ttcggattgc ggaattgcca ccacccgtac
    29581 cttggccgcg atcaccccaa agccggatcc ggtctcgctg gcgcccaaga catcgatgat
    29641 cgtgcccacc cggagcaggc cgagcgcgcc agcgtcctcg aagcggaccg gcagcgcgag
    29701 cttgccgtcc gccacctggc tgctgcctcc cagcagatcg gacgggctga gcacccggcg
    29761 gctgggtacc ggtgcgatca ccgtctgccc gacgaccgcg tccgcttcag tgaacgcctc
    29821 cgctggcaag actgatgcag gcacgcgcac cacgccgacg tccgcctcgg tcaccaccgc
    29881 gccgccggcg atcgtgcgga cggccaccac cgcgaacgtc ccggtgtccc cgcgcgcagc
    29941 cactaccgag agcgcggcga aaacggcgat cgccgcgaac aaggcggcga agaagcgtcg
    30001 atgccaacgg aaggcgcgac acaggctatg cagatggcga tgagcttgca cacccacatc
    30061 ttcggcaggt gagcggaatt tccgtcaccc atccacaggc tcaggctgcg gtgctcgccc
    30121 ctgtggatga cttgggcgcg gacgacttgg cgtcactcgt cgccgagcct gccgcagatg
    30181 tctcgacggg cttggccgca ttggtcttgc ccttggtgcg gttgtcggtg gcatagaagc
    30241 ccgaaccctt gaacaccacg ccgacggcgt tgtaaacctt gcgcaggttg ccgtcgcagt
    30301 cggggcaatc agtcagcgga tcgtcggtga acttctggac ggcttccagt tcgttcgcac
    30361 acgacgtgca gcggtactga taggtgggca tcttcacttc ctggttcgcg ctggccggca
    30421 ggttgctcgg tggcttcggt gccgcttggg aaggttcgcc aagggcgccg agggaaatct
    30481 taccgagcac ctagcgaaac tcggaaaccc gctcggctat tccgcagcgc acgtcaccag
    30541 ccgcgacggc gtgaccagga agtggacgcg ccgatcccag tcctccgtcg gcaggtcggg
    30601 cagcacctct tcgtcgaaga gcaacaggcc gagttgcgcg tccgatcggg cggagccgag
    30661 ggcgcggtcg taccagccac ccccggtgcc gagccggtcc ccagcggtgg tgccggccag
    30721 cccgggcagc cagatcacat ccgcttgggc gagcgcgtcc gcgcccagcg gccggccgct
    30781 cggctgccgg atgcccagcg gtccagcaac cagacccgcc tgacccgagt accaggccca
    30841 gtccggctcc cgggtgagca ccggcagcag cacccgaaca ccgctgtggt gcagccattc
    30901 caggagcagt gcggtgtccg gctcgtccgg acgcgagaca taggcagcga ccacagcagc
    30961 gcctgaggcg agcgtgaccg cacggctggt gcggctcgat tcggccctgg cgtcggcgat
    31021 ccgggccatc cgcgccgcgc gaagctgctc gcgcagcgcc gccttcgcga gagcggaatg
    31081 gctggctcgc ggctggcctt cgctggcgcc gggtgtcatt gacttatcgt aaagccatgc
    31141 cactcttcac ccgagccaag gaagaagcgc ccgccgaggc actggttcca gaacccaagc
    31201 tattgccggg tgcacccgat gccagcgccg ctgcggttcg cagtatcgac gatcatcggg
    31261 actacctgct ggcaaacgtg ccgagcctgc ggccgttcgg catcgggctg cttgacgcgg
    31321 ccgggctgac cctgtgcgag tcgatcaccg ccgacatcga tctgcccacc ttcacttcgg
    31381 ccacgacgga cggatgggca gtgcgcgcgg cgaacctggt cggcgccagc gaatcccgtc
    31441 cggtggtgct accggtggtg gacgagatcg cggccggtgg gttccgcggc gccccgctga
    31501 ccccgggcac cgcggtcaag gtcgagcagg gcgcacccat ccccgaaggt gcggacgcgg
    31561 tcgtcccgct ggccgatggc gtggtgctgg gcgaggacgt ccgcttcgtc ggcgaagcga
    31621 cgttccagca gaacctgcag atgctgggct cacggatctc cgacggcgac gcgctgctcg
    31681 aaaccggggt gaagctcacc ccgagggcaa tcgggctgct ggccgaagtc ggccacgaca
    31741 aggtgctggc gcggccgcgt ccgcgggtgg tggtgctcac cgccgcgcgc gagctggttg
    31801 aacccggact gccgctgacc caactgcatc aacgctacga ctccggcacg gtgctgctgg
    31861 ccgccagcgc gcgcaccgac ggggcgcagg tgttcccggc cgcgatcgcc gatccggagc
    31921 cgaaggcgct ggagcgatcg ttgtcggagc agttggtgcg ggccgacctg gtgctgctgg
    31981 tcgccgatgc caccgacgag ctggtgaacc tgctcggcac ggtcggcgcg gtcgacaccg
    32041 ctgaggtgga cgcgctgccc ggtcggcagg tgttctccct tgtgggcagc gagaaaacgc
    32101 cggtgctggt gctgccgaat gagccggtag ccgcctacct gacctacctg ctgttcgggc
    32161 gtccgttgct acagaagctg gctggcgtcg aggcctacct gcccaccgag gtgactgcac
    32221 cggtgaccac cgacatcgag gcggacgcgg accgcacccg gctgctgctg gccaggcaca
    32281 cctcccgtgg ggtgacgccg attccggtcg ctgtgccagg tgctgccgaa ctcgcggacg
    32341 cgaacgcggt ggtcgtcgtg cccgccggcg acaacatcgc agcgcacggc gacgtgtcgc
    32401 tgtggctgtt gtattgagcc cgggatgaga gtccactggc cggtacggct gactcacggc
    32461 gcaatcacat tgcgtccgtt gcgtcgccgg gacgaggccg agtgggcacg catccgccgc
    32521 cgtgccggag cgtggttcaa gccgtgggac tcctcccgtc cggctgatgc ggtcgaaccg
    32581 ggactgacgt tccggcagtt ggtggcgcag ttctcggcgc gtgcccggcg cggacagatg
    32641 ctgccgtggg cggtcgaata caccccgtcg gacacaaaaa ggcggctgtt cgtcggccag
    32701 gtgaccgtta ccggcatcac ccgcgggtcg gcctgctggg cgcagatcgg ctactggatc
    32761 gacccgaagt gggccggtcg cgggatcatc cccacggcgg tggcaatggc ctgcgactac
    32821 tgcttcacga cgctccggct gcaccgcgtc gaaatcgcaa tccgcccgga gaacacaaac
    32881 agcctcgcgg tggttcgcaa gctcggcttc cgctacgagg gacgccgcga gcgctacatg
    32941 cacgtggacg gcgactggcg cgatcacgac atgttcgtgc tgaccgcaga agaggtgccc
    33001 gaaggagtgc tcgcccgata cgaacgcgcc aagagcgtgc cgagcgctgg ctgagctggt
    33061 cgcgaccaag gaatattgcg ccaaaccgcc tgatttgctc gacactccgc ccgcggtcgg
    33121 ctggttcgag acgctcgcac aatacggttc gaggtgtggg aatgaccggg ctgatcttcg
    33181 ctgtgatcgc agccttctgg ttgatatacc tcattcccta cttcctgcac catcgcaacg
    33241 tcgaaaccgc cgaagatgat gcggacgcgg cgattccgtt caccccggac gtgacgatcg
    33301 ttcgcagcgg cacgtcgctg gctgaggccg atccggggac ggtgccggtt tcgacgccac
    33361 tcacccggcg agcacagctg cgcgagctgc ggctgcttga cgagcgtgcc gcgcagcgcc
    33421 ggcgacgggt gctgatgttc ctgctggtgg tgcaactgat cgtcgctggc ttggtggtgc
    33481 ttggcgtggg cgcttggtgg tcggcgctga ttccggccgg cctgatcgtg gcgttcctgg
    33541 tggtggccag attcagtgtc cggacgttgc gggcgggact ggctgagcgt gcccggaaga
    33601 tccgcgagtg cggcgatgag gagacggtgg ccatcgtgct gaccgccgat caggtggctg
    33661 agcatgagca ttccgtggag ctgagcgtgc cgatcggcaa ggtgggctcg ttgtgggatc
    33721 cgatcccgat cacgagaccc acttacgttt cgacgccgct ggcgccgcgc accgtccgta
    33781 ccatcgacct gtcgttcccg gtggccgccc cggcgcgtat gccggtgact gccgacccgg
    33841 tttcacccgc cgcggcgatc gagcctgagt cccaagagga cgtcggctag tcgagaccag
    33901 ccttgtcatc ccggcgcagg ccgggatctc cgaactgccg ccagtacagc gcagccccaa
    33961 tcagccgacc ccgcgaaatc gttcgtgctg tcgccagatg cgttgggttt gtccgggtcg
    34021 tagtggggca gcgaactcgg ggagtccgcg gtggtcgagt cggatcagcc actggcggtc
    34081 gggtggtccg ttcgggttgg gttcgacgag gtggtggtgg aagctgcaga gcaggacgcc
    34141 gttgtcgagg gaggtttgac cgccgtccca ccagggtttg cggtggtggg cctcggtttc
    34201 ggcgggtggc cggtcgcagc cgggtagggt gcagccgcgg tcgcgcagga tgagtagttg
    34261 gcgcagttcg ttggtgaaca gtcgcttctc ccggccgacg tccaagggga cggagttgcc
    34321 gttcatcacg accggcagga tgtcggcgtc gcaggccagc cggcgcgcgt ccgctgcggt
    34381 gatcggctgg tcggtgctga gcagggtggc ggtgccgagt ttgccgcaca gggtttcgtg
    34441 gtcgatggtg atgatgacgc ggggacggtc ggcgccgtac gtgggcgcga caccgcagga
    34501 ctggaagtgg tcgaccagca tgaccagggc gtctgctgcg gcttgggagc gggcgatcgg
    34561 ctcaccagtg agcagcacct gtttgcggtg cttgctggcc agcgcacgca gcaccccgat
    34621 gagctgttcg ccgacgaggg tgggtagctt gccgttgatg atccaggcgc cttcgaggtc
    34681 ttggcgccag gtgaggtacc ggtcgcggcg ctgttgcgcg tccaggcgtt caactgcctt
    34741 gcgatcggcg tcctcagcga tttcgggtgc cacgacttcc acggcccggt tcaccagccg
    34801 ggccaaaccg gacgggccga actcaccggc gaagccgacg agttgttggg tgatctgttc
    34861 gcgttgctgc tggtcgagct ggccgggtag ccgttcgagc ccgttgacga ttgctgcggc
    34921 ctgttcggtg gacatccgcc ctgcggcgag cgcgtcagcg atcatcgggg ccgcgaccag
    34981 cttcatggcg aggcgcactt cgttgcgggc ggaccgggcg gagtggctgt tctggttgac
    35041 cagccagttg ctggtgggga cgctgtcgcg gcgcagacag gcttcgcgtt tctcggcctc
    35101 agccagcagg gcgagccagc gggcgttgtg ttgatcgcgg acgcgtcggg cctgttcgag
    35161 ttcggccagc agctcggctt cgttgaactg ccacggagcg agttctgccg agggcatggc
    35221 ggcgagttcg gccgcgatgc agcagtcgag cagcgactgc agttcagcgg tgatctcggc
    35281 cgtatccatg tgtacgattc taccgcttga caacgccaaa gcacaatgta tttctgtcac
    35341 atctgtccca acttttcggc aggtgttggc tttcgtcctg gatctgcttc agcttggtca
    35401 ccccgcccac ttcagtcggc tatcccgcac acttcgcttg cgtcgtcccg ccacttccct
    35461 tcgccgtccc gccacttccc ttcgtcatcc cgccacttcc cttcgtcatc ccggcgcagg
    35521 ccgggatctc cctactgcct cagcgccgcg ccgggccgta cgatcgacgc catgccttcg
    35581 ctcaccgccc gtccggccaa cgcgatcctg ttcgaccgcg gtgtgctcac ccgggtccgt
    35641 cctcaggatg cggcggacgt gatcgccgcg gtcgcggcaa acgcagacca gttgcgcgac
    35701 tggattccct gggccgacgt tccggaagcc aggcgagcca aggcggaagg ggcggaagcc
    35761 gattgggagg ctgatcgcgc ctacgtctac gcattgcgag agacgccgct cggtccggta
    35821 atcggaggtc tcggtgccta ccgccgggtc gggcccggtg cgatcgagct gggctactgg
    35881 ctcgcgtccg agcacgtcgg tagggggctg gcgtcggccg ccacagccgc actcgcttcg
    35941 gtggtgtccg ccttgccgga cattgagcga gtcgaactgc acatcgacga ggcgaacgcc
    36001 cgaagtgtgg ccgtcgcccg ccggctcggc ttccggctcg cgcggacgga cgcctacgag
    36061 ccgcgaaccg cggccgagac gggtcgactg cagatctggg ttcgggcagt tgaaatggag
    36121 ccaggatcac cctccgcttg agggtcttgg cgagatcgtt acattcggtt gtcgtcaggc
    36181 gaggaattca ccgccgggtt gcggcgtgcg ggcgcctgac cagccaggcc caagcggggt
    36241 gcctcggcca tagatgggcc acggccgggg cggccggcac cgactgcgct gccagaagcg
    36301 gtttcgaccg ccaaagggcg cgatcgttgc ctcggcagag gcccgatttt gggcggtgag
    36361 tgcctttccg tgccggttct agctccagct acagggcgcg aacaattcac tgcggaattg
    36421 ccgatcgccg gcggacgagg tcgccgataa gccagcacgc gatgagtccg gcgagccagc
    36481 ttcgatagat attctggaat cgtttgacag tcgcctttgg aggcgagaaa gtgtgcgcga
    36541 gaacttcaag tgaagtcctc gctgcgcaac cggtcgaccc accccatccc ctcccagggc
    36601 cggtaaggga cattccgttt tgcgacgcaa gttttcaact agtgtcggga gtttttcatg
    36661 cgtgcaacgc tcaagcgtta ctttgacgcg ctcatcgcgc gtcgcgaaga gggcaaggac
    36721 gagggcttct cgctcatcga gctcatcgtc gtcgtcgcca tcctgggcat cctggt
//
LOCUS       DAOPRQ010000020        35034 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_948_length_35034_cov_11.320306, whole genome shotgun sequence.
ACCESSION   DAOPRQ010000020 DAOPRQ010000000
VERSION     DAOPRQ010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 35034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35034
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_948_length_35034_cov_11.320306"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1179
                     /gene="gltX"
                     /locus_tag="PKJ61_03995"
     CDS             <1..1179
                     /gene="gltX"
                     /locus_tag="PKJ61_03995"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014847264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOC13018.1"
                     /translation="EQLIASGHAYYCWCSSERLAELRAEQQAAKVSVTGYDRLCLGKT
                     REERAALPGFSEAPVVRMLIPDDAPLSFTDLIRGEVSAPRPDDQVILKADGFPTYHLA
                     VVVDDHLMGITHVVRGEEWISSTPKHLLLYRWLGWDEPAFAHMPLLRNTDKSKISKRK
                     NPAARLTWFVEQGYLPEALLNFLQLLGYPPVDAEHEVSSFDDFVANFEWSKVNTVGPI
                     FDLKKLDWLNGHYLRSLTPAELADRVVAFALRSGQWTDAPDAATVELLRSAVPLVQER
                     LVLLSEALPKLAYLFTADADLMIDPEALAKAGADASAVVAAATTALSGLGEWNHETIQ
                     AALREALVDGLGLKPKFAFGPVRLAITGSNVSPPLFESMELLGRESSLARLVKLQTLL
                     "
     gene            1176..1913
                     /locus_tag="PKJ61_04000"
     CDS             1176..1913
                     /locus_tag="PKJ61_04000"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOC13019.1"
                     /translation="MSEVVAIFGGRSEIGLAIATRLAPGATIVLAARPGGLGAALAAC
                     RDAGAARVETVEFDADDLDAQAGVVAEIEGRVGPIDVAVLAFGVLGNQALAEQDPLAA
                     AAVLNTDFVAQAALLTVLAERMRTRGRGTLVAFSSVAGVRVRRANYVYGSAKAGLDGF
                     ASGLADALHGSGVHLIIARPGFVIGRMTEGMEPAPFSSTPEQVADAVVRRIRSGRGVD
                     LWIPWQLRGMFAIARLVPRPLWRRMPR"
     gene            complement(2105..3037)
                     /locus_tag="PKJ61_04005"
     CDS             complement(2105..3037)
                     /locus_tag="PKJ61_04005"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOC13020.1"
                     /translation="MTLGLLASLAFATSGPFVRPLFDIGWTPGAAVFWRVAAAAVLMV
                     PVGLWLVRGRTRDLLAQWRAVLAFGVLAVATPQLMYFAAVSRMSVSIALLIEYMAPVA
                     LVLLAWVRTRHAPSRLVLSGTLAAVLGLLCVLDLTGATPDALGVLFGFGAMIGAAAYF
                     VLSARPTTLPPIALAAFGLAAGAVVLGLALLVGLLPYSVAFVPVQLLGLSVAWWVPLG
                     VVVVSATALAYALGVAGISLMGERLASFVSLSEVLFAALLAAVVLGEVPSAIQILGGV
                     LIIFGVLLIRFASGRAPAGLPTLPDELEAAESGH"
     gene            3359..3670
                     /gene="rpsJ"
                     /locus_tag="PKJ61_04010"
     CDS             3359..3670
                     /gene="rpsJ"
                     /locus_tag="PKJ61_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014847259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HOC13021.1"
                     /translation="MAGQKIRIRLRAYDHEVIDSSAKKIVDTVTRTGAKVAGPVPLPT
                     EKNVFCVIRSPHKYKDSREHFEMRTHKRLIDILDPTPKTVDSLMRLDLPAGVDIEIKL
                     P"
     gene            3682..4341
                     /gene="rplC"
                     /locus_tag="PKJ61_04015"
     CDS             3682..4341
                     /gene="rplC"
                     /locus_tag="PKJ61_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016455369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOC13022.1"
                     /translation="MSERNVKGLLGTKLGMTQVWDENNRVVPVTVIQAGPCVVTQVRT
                     PDADGYSAVQLGFGAVKAKKVTKPAAGHFEKAGVTPRKHLVELRTGDASTYTLGQELT
                     ADVFDAAEIIDVTGVTKGKGTAGVMKRHGFHGLRASHGVHRKHRSPGSIGACATPGHV
                     FKGTRMAGRMGVEKVTVQNLTIHAVDAERGLILVKGSVPGPKGALVVLRTAAKKDGAK
                     A"
     gene            4338..5003
                     /gene="rplD"
                     /locus_tag="PKJ61_04020"
     CDS             4338..5003
                     /gene="rplD"
                     /locus_tag="PKJ61_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HOC13023.1"
                     /translation="MSETRTVKVLGGKKSATAELPAELFDVQTNIPLIHQVVVAQQAA
                     ARQGTHSTKTRGEVAGGGAKPWRQKGTGRARQGSRRAPQWTGGGVVHGPQPRSYAQRT
                     PKKMIAAALRGALSDRARDGRVHVVDSIVTGETPSTKAALKALAAIGGSKLLVVLDRD
                     EDVAWLSLRNVASVHALAVDQLNAYDVLLSDDVVFTTAALAVFAGAPSAAEAAIETEE
                     DAK"
     gene            5000..5308
                     /gene="rplW"
                     /locus_tag="PKJ61_04025"
     CDS             5000..5308
                     /gene="rplW"
                     /locus_tag="PKJ61_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002523451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HOC13024.1"
                     /translation="MSDLKIRDHRDILLAPVVSEKSYGLLDENKYTFLVSPSANKTEI
                     KIAVEAVFGVKVTGVNTSNRQGKRKRTKAGWGKRPDTKRAVVSVAEGQRIDIFSGPLA
                     "
     gene            5338..6174
                     /gene="rplB"
                     /locus_tag="PKJ61_04030"
     CDS             5338..6174
                     /gene="rplB"
                     /locus_tag="PKJ61_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015889918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOC13025.1"
                     /translation="MGIRKYKPTTPGRRGSSVADFVELTRSTPEKSLLAPKPKTGGRN
                     NSGRITTRHIGGGHKQAYRLIDFRRYDKDGVPAKVAHIEYDPNRTARIALLHYADGEK
                     RYIIAPNGLAQGALVEAGEGADIKPGNNLPLRNIPVGTTVHAVELRPGGGAKMGRSAG
                     AKIQLVAREGKMATLRLPSGEMRLVDVRCRATIGEVGNAEQSNINWGKAGRSRWKGVR
                     PTVRGVAMNPIDHPHGGGEGKTSGGRHPVTPWGKPEGRTRDKNKASSRLIIRRRKSGK
                     KR"
     gene            6197..6478
                     /gene="rpsS"
                     /locus_tag="PKJ61_04035"
     CDS             6197..6478
                     /gene="rpsS"
                     /locus_tag="PKJ61_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002516048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HOC13026.1"
                     /translation="MPRSLKKGPFVDDHLVKKVDAQNEKGTKQVIKTWSRRSMITPDM
                     IGHTIAVHDGRKHVPVFVTDSMIGHKLGEFAPTRTYRGHVKEDKKSRRR"
     gene            6505..6903
                     /gene="rplV"
                     /locus_tag="PKJ61_04040"
     CDS             6505..6903
                     /gene="rplV"
                     /locus_tag="PKJ61_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002520596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HOC13027.1"
                     /translation="MSNKERPTRRAALLGDRPGSYAIARHVRMSPMKVRRVVDLVRGM
                     PVTDALDVLKFAPQAASEPVYKVVASAAANAESTEGLRREELVVSAAFVDEGMTLRRI
                     RPRAKGSASRILKRASHITVVVEPKTEKGV"
     gene            6906..7730
                     /gene="rpsC"
                     /locus_tag="PKJ61_04045"
     CDS             6906..7730
                     /gene="rpsC"
                     /locus_tag="PKJ61_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014847252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HOC13028.1"
                     /translation="MGQKINPHGFRLGITTDHKTRWYADKNYSDLVAEDVKIRRYLAK
                     TLERAGISSVEIERRSERVTIFLYAARPGIVIGRNGAEAERVRAELEKLTGKQVQLNI
                     LEVKNPETDAQLVAQGVAEQLGARVAFRRAMRKAQQSAMRAGALGIRIQCSGRLGGAE
                     MSRSEFYREGRVPLHTLRADIDYGFYEARTTFGRIGVKVWIYKGDVSGNRAERAAQKA
                     ARQAAGGQRRPARGPRRDGGAGAGRGERTERGGRRRAEGVAEGAAAPEAVESNAGA"
     gene            7733..8152
                     /gene="rplP"
                     /locus_tag="PKJ61_04050"
     CDS             7733..8152
                     /gene="rplP"
                     /locus_tag="PKJ61_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014847251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HOC13029.1"
                     /translation="MLIPRRLKHRKQHHPKRSGAAKGGTKLAFGDYGIQALESSYLTN
                     RQIESARIAMTRHIKRGGKVWINVYPDRPLTKKPAETRMGSGKGSPEWWVANIKPGRV
                     LFELSGVGEDVAREAMRLAIHKLPFKARFIKREAGDI"
     gene            8152..8382
                     /gene="rpmC"
                     /locus_tag="PKJ61_04055"
     CDS             8152..8382
                     /gene="rpmC"
                     /locus_tag="PKJ61_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014847250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HOC13030.1"
                     /translation="MATSVTANELRGLSRDQLNAKVVELKEELFGLRFAAATGQLESH
                     GRLREARKDIARIYTVLQERNLGIVPDPDEDK"
     gene            8388..8654
                     /gene="rpsQ"
                     /locus_tag="PKJ61_04060"
     CDS             8388..8654
                     /gene="rpsQ"
                     /locus_tag="PKJ61_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002514860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HOC13031.1"
                     /translation="MSDNEKRGARKVREGIVVSDKMDKTVVVAIEQRRKHPLYGKVMT
                     SSERLKAHDEANEAGVGDRVRVMETRPLSATKRWRLVEILEKAK"
     gene            8671..9039
                     /gene="rplN"
                     /locus_tag="PKJ61_04065"
     CDS             8671..9039
                     /gene="rplN"
                     /locus_tag="PKJ61_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HOC13032.1"
                     /translation="MIQQETRLKVADNTGAKELLCIRVLGGSKRRYAGLGDKIVATVK
                     DAIPGGNVKKGEVVKAVIVRTVKETRRPDGSYIKFDENAAVILKNDGEPRGTRIFGPV
                     GRELREKKFMRIISLAPEVL"
     gene            9042..9419
                     /gene="rplX"
                     /locus_tag="PKJ61_04070"
     CDS             9042..9419
                     /gene="rplX"
                     /locus_tag="PKJ61_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013160501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOC13033.1"
                     /translation="MANTLHVKKGDRVKVIAGKDKGRIGEIISVDPQAGRVIVQGVNL
                     VKKHRRETPNNSTRGNNAGGIITTEAPIDASNVQLVVKVDGKDVTTRVGYERQVVTKR
                     RADGTEYTAERSVRIARKTGKEI"
     gene            9419..10048
                     /gene="rplE"
                     /locus_tag="PKJ61_04075"
     CDS             9419..10048
                     /gene="rplE"
                     /locus_tag="PKJ61_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015071230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOC13034.1"
                     /translation="MAATATKVLPRLQEKYRSEIVPALNTEFGYENVMQIPGLVKIVV
                     NMGVGEAARDSKVIDAAIRDLTAITGQKPTVTKARKSIAQFKLREGMPIGCHVTLRGD
                     RMWEFADRLLSLALPRIRDFRGLSARQFDGKGNYTFGLTEQVMFHEIDQDRIDKLRGM
                     DITFVTTAKTDDEGRALLKALGFPFKAPADEVAKVVKKKGPAFTGKKKR"
     gene            10057..10242
                     /locus_tag="PKJ61_04080"
     CDS             10057..10242
                     /locus_tag="PKJ61_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003948630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HOC13035.1"
                     /translation="MAKTALKVKQARKPKFAVRAYTRCNKCGRPKAVYRKFGLCRICV
                     RELAHAGQLPGVIKSSW"
     gene            10325..10732
                     /gene="rpsH"
                     /locus_tag="PKJ61_04085"
     CDS             10325..10732
                     /gene="rpsH"
                     /locus_tag="PKJ61_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOC13036.1"
                     /translation="MTMTDPIADMLTRLRNANQAYHETTSMPHSKIKVGIAEILKAQG
                     YISGFEVLEPGAGEVGKTLKLTLKYGQNRERSIAGVRRISKPGLRVYAKSTALPKVLG
                     GMGIAIISTSQGLLTDKEANTRCVGGEVLAYVW"
     gene            10750..11292
                     /gene="rplF"
                     /locus_tag="PKJ61_04090"
     CDS             10750..11292
                     /gene="rplF"
                     /locus_tag="PKJ61_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002514854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOC13037.1"
                     /translation="MSRIGRLPIAIPAGVDVTLDGQTVSVKGPKGALAHTVKEPIKVG
                     RNDEGQLEVTRPNDERESRSLHGLTRTLVSNMVIGVTDGYEKKLEIVGVGYRVIFKSP
                     EQLEFNLGFSHPVVVNAPAGITFSVESNTKFTVYGIDKQAVGEVAANIRKIRKPEPYK
                     GKGVRYAGERVRRKVGKAGK"
     gene            11292..11669
                     /gene="rplR"
                     /locus_tag="PKJ61_04095"
     CDS             11292..11669
                     /gene="rplR"
                     /locus_tag="PKJ61_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012923671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOC13038.1"
                     /translation="MAISLSTNKGMADRQAARLRRQARGRKKIAGSPERPRLVVFRSS
                     RHISAQVIDDAAGVTLASASSLELRGADGDKSAKAKEVGKLVAERAKKAGVENVVFDR
                     AGNKYHGRIAALADSAREAGLGF"
     gene            11693..12322
                     /gene="rpsE"
                     /locus_tag="PKJ61_04100"
     CDS             11693..12322
                     /gene="rpsE"
                     /locus_tag="PKJ61_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOC13039.1"
                     /translation="MNGTQRRGGGAGTGTGGEQRRGGREDRRGRDAAAEKDKSNYLER
                     VVAINRVAKVVKGGRRFSFTALVVVGDGDGMVGVGYGKAKEVPAAIAKGVEEAKKQFF
                     RVPRIQGTIPHPVQGEKAAGVVMLRPASPGTGVIAGGSARAVLECAGLRDVLAKSLGS
                     DNAINVVHATVAALQSLEEPEAVAKRRGLPVADVTPAAILRAREEGAHA"
     gene            12319..12501
                     /gene="rpmD"
                     /locus_tag="PKJ61_04105"
     CDS             12319..12501
                     /gene="rpmD"
                     /locus_tag="PKJ61_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002514851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HOC13040.1"
                     /translation="MSRLKVTQIKSGISGKQNQRDTLRSLGLKRIGDVVVIEDRPELV
                     GMVKTIPHLVSVEEVE"
     gene            12503..12943
                     /gene="rplO"
                     /locus_tag="PKJ61_04110"
     CDS             12503..12943
                     /gene="rplO"
                     /locus_tag="PKJ61_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020388091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HOC13041.1"
                     /translation="MALKVHHLRPAPGAKTEKTRVGRGEGSKGKTAGRGTKGTGARKN
                     TPENFEGGQMPLHMRLPKLRGFNNPFRVEYQVVNVARIAELFPAGGEVTPAELVAKGA
                     VRDGQLVKVLGNGDLTVAVQVSAHAFSASAKEKIAKAGGSVTEL"
     regulatory      13072..13136
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01763"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="guanidine-III (ykkC-III) riboswitch; guanidine
                     riboswitches control genes whose products are involved in
                     modification or pumping out guanidine as a toxic compound
                     from bacteria; this is a third class of guanidine
                     riboswitches based on ykkC-III RNA motif; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="guanidine"
                     /db_xref="RFAM:RF01763"
     gene            13149..13463
                     /locus_tag="PKJ61_04115"
     CDS             13149..13463
                     /locus_tag="PKJ61_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family transporter"
                     /protein_id="HOC13042.1"
                     /translation="MSWLVLVLSGILESVWAIALGRSEGFSRPLPTVIFGVALLFSMG
                     GLAYAMRELPTGTAYAVWVGIGATITVAYGMFTGEEPVSVLRILLLGVIVAGVIGLKL
                     LH"
     gene            13616..14938
                     /gene="secY"
                     /locus_tag="PKJ61_04120"
     CDS             13616..14938
                     /gene="secY"
                     /locus_tag="PKJ61_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020388090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOC13043.1"
                     /translation="MLSAFANALRTPDLRRKILFTLMILAVFRLGSVIPTPNVNIVNV
                     DQCRTLATSGDSAGLYSMIQLFSGGALLQLAIFALGIMPYITASIILQLLTVVIPRLE
                     ALKKEGAAGTAKITQYTRYLTLVLAVLNSTAFVTLAVNGGLFQGCSLDLVYDKGLFAI
                     ITMILTMTAGTGVIMWLGELITDRGVGNGMSVMIFTQIAAQFPASLWSVKESREGAQG
                     WLLFILVLAVGLAVMAGVIFMEQAQRRIPVQYAKRMVGRRLLGGTTTYIPIKVNQAGV
                     IPVIFASSMLYLPVLYAQFQPGTVDNPNPVSAWISANLAQGSWVYELLYFVLIIAFAY
                     FYVSITFDPVEVSDNMKKYGGFIPGVRAGRPTERYLAHVLSRLTAPGSLYLALVALIP
                     AIAFVLLGTHNKFPFGGTSILIIVGVGLDTVKQIQSQLQQRNYEGFLR"
     gene            14935..15504
                     /locus_tag="PKJ61_04125"
     CDS             14935..15504
                     /locus_tag="PKJ61_04125"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HOC13044.1"
                     /translation="MRLLIMGPPGVGKGTQATGIAAHYGVPAISTGDIFRANVKNQTP
                     LGQEVSRIMADGGYVPDVITNAIVADRLTEADAANGWLLDGYPRTIGQVEALDEALGE
                     GPGIDAVISLVADTDVLVDRLLKRAELEGRADDNAETIRNRMVIYTEATDPLLQTYRD
                     RDLLVEVDGIGGIEEVGQRITAALDAKLS"
     gene            15529..16371
                     /gene="map"
                     /locus_tag="PKJ61_04130"
     CDS             15529..16371
                     /gene="map"
                     /locus_tag="PKJ61_04130"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012923665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOC13045.1"
                     /translation="MRRRPGIELKSADQIRRMRRAGLVVGRTLAAVRAAAAPGVTTAE
                     LDAIAREQIAAAGALSSFLGYGGGFGLPPFPAVTCISVNSEIVHGIPGERVLASGDLV
                     SVDFGVSIDGWHGDSAVTFGVGELSPEASALSEATREAMWAGIASARIGGRIGDIANA
                     IETSIHSHSVGYGIVSEYTGHGIGTAMHQPPDVPNLGRAGRGELLAKGICLAIEPMVT
                     LGSANTATLEDEWTVVTRDGSVAAHWEHTIALTPAGVWVLTAADGGEAELTARGVKFG
                     PLAD"
     gene            complement(16639..17760)
                     /locus_tag="PKJ61_04135"
     CDS             complement(16639..17760)
                     /locus_tag="PKJ61_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13046.1"
                     /translation="MREFHICTAAPGPDWVYAGYHELDAAISVEMYDEDNSTPTDQVQ
                     AWHADQVHGRKALLLALPGPAPADALIGRYGLPVVPAEPVKVLGITEIYLPTTDNLHL
                     LDNVSIMVRADQRRAGIGAALAREVRRIAVDEGRDTIVGWSQHSPGAPARADDLVGPD
                     GGGHVPLDHVSRFALAAGYSLAQVERQSRLLLPLAPELLAQLLAEAEAKASPEYRAIS
                     WAGPTPAELRDGVARMHAALSTDAPLGDVDWQPEIWDAARVQQMEERVHRTSRLLTTV
                     AVEVASGDVVALTDLHLPNAHPQRLEQGTTTVDRAHRGHRLGLLVKVANLQLLAQTQP
                     DAVHVDTWNAGENEWMLAINTALGYRRYTNFGIWQAKLG"
     gene            17831..18352
                     /locus_tag="PKJ61_04140"
     CDS             17831..18352
                     /locus_tag="PKJ61_04140"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HOC13047.1"
                     /translation="MSTTAKRRVLAGLVDLGLMLLWVAVVAAVGVPLYLTGVTATVGP
                     LAQNLIGLLCVVVPVTLGLTLAECGRYEATPGKLKFGLRVRTDTGDRVTWPRSLLRNL
                     LKLGLPWTLGHAGAVAVAYGGGWDAQLGAVLSLLVPVAYLVSLFAGSGRTIYDHLAGT
                     TVIETAPGRRFAD"
     gene            complement(18370..19710)
                     /locus_tag="PKJ61_04145"
     CDS             complement(18370..19710)
                     /locus_tag="PKJ61_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020386775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOC13048.1"
                     /translation="MKRLSLPSWLTRLPSWLRRGRVADAGATAVNSASALLSPVARAD
                     RFGFADLLMEATAGIGSRPGRLVMTILGTVLGIGSLIATIGFAQTAAGQIARQFDAAA
                     ATQVVITPKTSRTGSGASVATVRLPWDASERVGRLAGVQSAALFSEVGLGEGTVTAVP
                     VNDPSAASAAVPAVVAASPGLMDALSGHIVTGRCFDSGHDQRADRVAVLGVRVAERLG
                     VSRVDRQPSIFISGLAYAVIGIADDFQRKAELKDSVIIPMGAGREDFGVTAPGELQVK
                     IVVGAGPQVGKQAAIALAPDAPENIEVAAPSGHSELSTNVQADVNIVFLVLGVVALLA
                     GGLGIANVTMLSVLERVGEIGLRRAVGATGRQIAAQFVLESVLIGLLGGLIGASLGVF
                     AVVGVALIQQWTPIIDPWLAVAAAALGAVVGLLAGWFPAHRASRVEPVNALRAG"
     gene            complement(19710..20390)
                     /locus_tag="PKJ61_04150"
     CDS             complement(19710..20390)
                     /locus_tag="PKJ61_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012918215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOC13049.1"
                     /translation="MTATPLVELVDLTRAFPGPPEVQALKDVNLNIAAADYVSIVGPS
                     GSGKSTMLNILGLLDRPSVGEYRLAGTPTRDLTEDDRAAVRARVVGFVFQAFHLLPNR
                     SVLDNVLLPMLYSGVPRAEREDRARTALSRVGLAHRVNFLPGTLSGGERQRVAVARAV
                     VSGPQLLLADEPTGNLDQGTSGEVMSLFEELHADGLTLVVITHDAAVAKRATRRIRIL
                     DGRLTEAA"
     gene            complement(20387..22003)
                     /locus_tag="PKJ61_04155"
     CDS             complement(20387..22003)
                     /locus_tag="PKJ61_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13050.1"
                     /translation="MARRRPGAADAEPVAQGEALAAAADDPESPDSPDPQAAAAASPG
                     HGRAAGWLRIFRENRALWVTAAVATACLVAGLVVGRFVLSPADAAARTAAPEAGYITV
                     PVAFGTLTNDVTMRGQVGYADSTEVKIAAPEGADAAVVTGQVPKAGAELDAGDIALEV
                     AGRPVFVLPGKLPAYRTLRVGTSGPDVKQLKQALADQGISVTSGSKFDNDTAKAVRTL
                     YLRAGYTAPVDSTAAEGVRTAREALRSADSGLASARKALAEARKGPSAVTRKEADNAV
                     AEARRALQAAQAADSPDALEVARLKDELALAKLRRKEALKRGDTSAEAGALSSAKQQR
                     KDAAKQLADAKKAALPYLPAGEVLYLTQLPRRVDEVKVERGDILDGAAMSVSGAKLEL
                     SGSVSAADAKLLEVGKDAFFELPDGNQHRAVIATIEAADKGDRSTIGFTPDPLTAEQT
                     EQLKGSNVKLTIAVGSSDGDVLSVPLAALTAGPGGEARVEVVEGDPRDKAQAKTRLVV
                     VRTGLAAAGYVEVAPVAGSLVKDDLVVVGT"
     gene            complement(22003..23055)
                     /locus_tag="PKJ61_04160"
     CDS             complement(22003..23055)
                     /locus_tag="PKJ61_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13051.1"
                     /translation="MPRPRRLTSAIAIAAFTLAGCTGAPGLGAPSALKAEDSPLTKYL
                     ANLSLGPGPDATKEEQQAYYDEQQKKTEQLVAECMKKEGFEYKPNTTTSVTVGGTEEN
                     PWKPDDREWVKQYGYGSVNFPGKDDAPVEEPTGTEQVDPNQEYLASLTEAEQQAYNEA
                     LYGGQAATAEPTDAEPTDEAQTWDWTKQGCYGAAQHEVMGDQPYEKAEFKPLIDALQK
                     FYEGLPTNTEYSALDSEWVACMDTAGHTGFKTQSEASNSIYDLVNKYYEEAYPADGGE
                     ETEMDPNLGTLKDPAFAEIGKKEVELALADLDCREKTDYRNRQLTIQFKLEDQFISDH
                     KAELDALKASADQGKR"
     gene            23296..24009
                     /locus_tag="PKJ61_04165"
     CDS             23296..24009
                     /locus_tag="PKJ61_04165"
                     /inference="COORDINATES: protein motif:HMM:NF014741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HOC13052.1"
                     /translation="MDERLRDLPVREALARIRAVLDRVNPDRKALAPQDRLFLATAAR
                     SLTGRLEALGSLLLAGADRANASERSAGTPTSTWLALDQNLTKREAAGALHRATELAA
                     HPALGEAAVAGRVGTGQVRAINTVLSGLAAQLNDQQQAQAEELLVGLAACMDSDVLGK
                     AAAQVLAKVAPADADEVLETKLQREAEAAHRPAVWAGREACPPTAERRRLSFTEPSSS
                     SGIGGRAATGQASWRPEAA"
     gene            complement(24054..25277)
                     /locus_tag="PKJ61_04170"
     CDS             complement(24054..25277)
                     /locus_tag="PKJ61_04170"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HOC13053.1"
                     /translation="MIGIVGSGLAGLAAAARLSREGHQVVVFDPGASFEPGVDGLGQS
                     GGWAQAAGIEVASEPDHDVFTLPAAWRDLFYKSGRTLDAELAKRGLGLAPAPAREYRF
                     SNGATLALPSDRGEQWTVLGEALGEPAAVAWRDLLDHLDDVWQVQRRLGLEAEFTGLE
                     QLTAEVREVLRPRESLARLAGRLPTAELSEMVLDVAARLGQDPRRLPGWHAARLSVER
                     TFGRWRLVDGAGAPQPANRLVELLLERVAGCGVEIRTEAVSAIRRDARGLRCDTAFGS
                     TYLNAVISTLDPAAQVDLTQNRTDGRVAARLVRSPSGGPRWDSWRTLLSLPKLTTGVP
                     GVLAASAWSPGGPDAWAQLLTGALVAYRVHSDLTGADIRPTNKAYRRPRPIPRPRPTR
                     SAPEASAPRLASRED"
     gene            complement(25274..25855)
                     /locus_tag="PKJ61_04175"
     CDS             complement(25274..25855)
                     /locus_tag="PKJ61_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HOC13054.1"
                     /translation="MDVVALTFASGWASGINAYATVFLLGVLGRFAGISEIPDGFQRT
                     DVLIVMGVLAAAEFIADKVPYLDSAWDGISTFIRPVAGALIGALLAGASGELLPITLG
                     ALGGVTALISHAAKSSVRLAVNTSPEPFSNVGASLTGDVALVGVVSLAIAYPVQAAII
                     AALLLFATIALAVLLIAQSRKGVIAFRAWLART"
     gene            26010..27206
                     /gene="dinB"
                     /locus_tag="PKJ61_04180"
     CDS             26010..27206
                     /gene="dinB"
                     /locus_tag="PKJ61_04180"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HOC13055.1"
                     /translation="MRIDGDGARADRTIMHVDMDAFYASVELRRRPELREVPMWVGGA
                     GRGVVLSANYPARAHGVRGGMSSTAARRLCPQAVSLPPDFDAYTEVSDGVFAIFEAFT
                     ARVETASIDEAYLDATGALNPGLSASALGELLRATVQDEQAITCSVGIGPNKLVAKMA
                     SNAAKPDGLLEVPPEAVVAFLHPQPVEHLVGVGESTAARLRQLGIHTIGELAKAERTG
                     LRQVFGPRAGMLLSELAWGRDSSRVVARPGERGVGCQETFGRDTDDSNTVQTELLRVS
                     VKVASRMRAAEVLGTTVTLSLRYADFSTVSRSLTLAAPTDRTDEIHGAALRLLARLVP
                     QRTRIRRVGVRVTGLKDRARVYLQPTLDAPEHGWREAEVAADKAIRKFGPNAVRRAAL
                     TGRQRP"
     gene            27295..27663
                     /locus_tag="PKJ61_04185"
     CDS             27295..27663
                     /locus_tag="PKJ61_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3040 domain-containing protein"
                     /protein_id="HOC13056.1"
                     /translation="MALSDEEQRLLDQMEAALAAEDPKLANTLRGSTRQWQRRRLIIS
                     GLGFVLGVVALIGGMELHPVISVLGFLVMLASAAVAVTAWQRSGAAADGHGQGGAGSD
                     RDFMGKMEERWRRHQDESGL"
     gene            complement(27676..29796)
                     /locus_tag="PKJ61_04190"
     CDS             complement(27676..29796)
                     /locus_tag="PKJ61_04190"
                     /inference="COORDINATES: protein motif:HMM:NF023418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HOC13057.1"
                     /translation="MTGKDRISLAAVAAMLSAGLALTPLTTDRTYLMLALVLMLTSAG
                     VGAAVRRLTESELTVRITQLLPAGLLLWLAPATREPIELIEETVGFVQESVAPMEFHI
                     GFAVFCVLLLWLLYLVVESMAAGLGTPAWTFPALFTPYLITALVAYLEAPPWLFLLPA
                     AGYALLLATDTRNRAEPDSGDHVPTGPERMAAESWRSGVARAALAASVAALVGTLIAG
                     LAIPERYRDWSGPSGPGAVQLGDPSLDLIRNLNSTSDKPVITYRTSSETGEYLRLAAL
                     PVLDNTGFHLSATELVGLPLRNNIAEDLYSDRSRTEIEVGEFASEYLPVPWAPLNVAV
                     AGEWRYDPRTLAVVAGGDDRRTASRKTSYSVVSARMFSLDQLLPQLTEAGAVGDDGIS
                     LNVPEGISPDVVELTQTLFEGTTTAGERAIAIRDHLRSGEYTYSTVTAPGTTMDTLND
                     FLVGRRIGYCEQFAGAMAVLSRMAGIPSRVVIGFLPGTRDGQRWQVSPRNMHAWAELY
                     FGDDIGWIAVDATPPGAVAGNGTPSASPSARPTPTTASPSAASASPSATAAPTAPGGF
                     GLPPAWLGWTALGLAVVALAGFGPGVARRTLRSRRLRDSGLPFESAWEELAALAKDRN
                     IPWPDGSSRAVAVDLAPELAGPARVDFERLALDLQRSRYSAEPVPVAELTARVERIEA
                     AMKKRWAEPTAWLDRWWPRSLRPW"
     gene            complement(29793..31004)
                     /locus_tag="PKJ61_04195"
     CDS             complement(29793..31004)
                     /locus_tag="PKJ61_04195"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOC13058.1"
                     /translation="MRSGAGLTGRGWTMLGAGVLWCVGAGFVGQRDLWWPGLFLILLP
                     VLSWLLLLPNATGLSARRSVAPSRVIVGGQTEVEVMLAPRTRMLGGVTRVRDRVPATL
                     GQTSWYGLPTRAGRWVQRLRYQLSPSRRGRYRIGPLERTSGDGLGLARAAQVVPAYSE
                     LLVTPRVEVLANLGAASGLGVAPDTTLLRTGHGSADDVLIREYHHGDDMRRIHWRSTA
                     RTGELMVRREERSRDPIATIVLDNRARSYGGHAADERFEWAISAAASIGVHLLADGFD
                     VSLVMADGSLVSPPRAGADRENVLLEHLAEVDTVGNRHLSEAMATCTRGEEGQLLIAV
                     LGRVEAADSVALTEACQRGRACWALLVNSGGRDVIGLNTLVAAGWRCGTAEAGVGVAD
                     AWRNLGRQESR"
     gene            complement(31021..31980)
                     /locus_tag="PKJ61_04200"
     CDS             complement(31021..31980)
                     /locus_tag="PKJ61_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOC13059.1"
                     /translation="MTSTDTEIGEVLALAQAVRATVSQVIEGKPEAVDLAITALLAES
                     HLLIEDVPGVGKTMLAKSLARAIDCTVRRIQFTPDLLPSDITGVSVWAAGEFQFKPGG
                     IFANIVIGDEINRASPKTQSALLEAMEERRVSVDTTTHLLPRPFLVIATQNPIEMEGT
                     YPLPEAQRDRFGLRISMGYPSTEAEVAMLDHHGERDMLEPLVPVTDAAAVMRAVQVVR
                     RVFVHSSLKSYLVGIVNETRSQSATRLGASPRAGLQLMRAAKARAAMSGRNYVIPEDI
                     SDLAEPTLAHRILLNAQAQRGPNSSSELIHACVRAVPVPTGRR"
     gene            32142..32570
                     /gene="mraZ"
                     /locus_tag="PKJ61_04205"
     CDS             32142..32570
                     /gene="mraZ"
                     /locus_tag="PKJ61_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HOC13060.1"
                     /translation="MFLGTYTPKLDDKGRFFLPAKFRDQFGSGLVITRQQDRCLAIWP
                     TEAFMAEVAAAATGPSTVRGVREYQRMMASGASDESPDSQGRVTVPVPLRAYAGLAKD
                     IVVIGAFNRLEVWDATTWAEYSAAQEEAFAALDDVAPAAG"
     gene            32713..33687
                     /gene="rsmH"
                     /locus_tag="PKJ61_04210"
     CDS             32713..33687
                     /gene="rsmH"
                     /locus_tag="PKJ61_04210"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015070006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOC13061.1"
                     /translation="MAEVTGGAASLHVPVMAERILELLAPALGAPGSVYVDGTLGMGG
                     HAQLVLSACPQARLVGIDRDPDALALAGERLAPFGDRVELHHAVYHELPDVLADAGLK
                     RVQAICLDLGLSSLQIDRAERGFAYSVDAPLDMRMDPGAELSAASIINTWPAKELARI
                     LRVHGEERFANRIADRIVAARADAPFTTSARLVQVIADAIPAAARHTGGHPAKRTFQA
                     LRIVVNGELESLAGVLPAGLSALALGGRMAVLAYHSGEDRLVKQAFAKASSDRVPPGV
                     PAVPAGYAAEFSPLTRGAERPSAAEIEANPRAASARLRAITRNQEVDR"
     gene            33684..34370
                     /locus_tag="PKJ61_04215"
     CDS             33684..34370
                     /locus_tag="PKJ61_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13062.1"
                     /translation="MTALLAPRPGKQGARPQLRPVARPRGRMATIPFALVIALVLAAG
                     TVGLLVLTTALQNQAFAVQSRQHEATVLEIQLASLQAEVADARSIQHLAVAAQQLGMR
                     PNPYGAQLRLSDGKVLGEASAVVGGEVPTARYLTKEQAEAQVIALDKAEAERIAKAKA
                     EAKQRAEAKKEAEAKKKAEAKKKAEAEKKAEAKKKAEAKKKAEAKKKAEAKKKAEAAK
                     KAKAKKGKQP"
     gene            34367..>35034
                     /locus_tag="PKJ61_04220"
     CDS             34367..>35034
                     /locus_tag="PKJ61_04220"
                     /inference="COORDINATES: protein motif:HMM:NF015662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOC13063.1"
                     /translation="MSRPLDRFFASDASKKKRKVKRLPLAPTVGRLRVLLISIAVVFS
                     LAAGRAVQVQAIDATNVAEQAANQITVAHDLPAFRGEITDRNGEVLAMTADTVKIIAD
                     AKMIATNGRMNAEMTTKDREVAAAAPQQIADLLVRFLGGTADEYLPKLTATGKGQSYQ
                     LIAKNVSGATYREIALAMTAAGLVGLRSESNPTRVYPNGALASNVLGFLSEDGTGASG
                     LEQAL"
BASE COUNT         5812 a        11753 c        12120 g         5349 t
ORIGIN      
        1 gagcagctga tcgcgtccgg gcacgcctac tactgctggt gctccagcga gcggctggcc
       61 gagttgcggg ccgagcagca ggccgcgaag gtttcggtga ccggctacga ccggctgtgc
      121 ctcggcaaga cccgggagga gcgggccgcg ctgccgggct tcagcgaggc cccggtggtg
      181 cggatgctga ttccggacga cgccccgctg agcttcaccg acctgatccg gggtgaggtg
      241 agcgccccgc gtccggacga tcaggtgatc ctgaaggcgg acggtttccc cacttatcac
      301 ctggccgtgg tcgtggatga ccacctgatg ggcatcaccc atgtggtgcg cggcgaagag
      361 tggatctcgt ccacgccgaa gcacctgctg ctgtaccgct ggctgggctg ggacgagccg
      421 gcgttcgcac acatgccgct gctgcggaac accgacaagt ccaagatctc caagcgcaag
      481 aacccggcgg cccggctcac ctggttcgtc gagcagggtt acctaccgga ggcgctgctg
      541 aacttcctgc agctgctggg ctacccgccg gtggacgccg agcacgaggt gtcgagcttc
      601 gacgacttcg tggcgaactt cgaatggtcg aaggtgaata ccgtcgggcc gatcttcgac
      661 ctgaagaagc tggactggtt gaacgggcac tacctccgct cgctgacgcc ggccgaactc
      721 gccgatcggg tggttgcatt tgcgctgcgc tccggccaat ggacggacgc gcccgatgcg
      781 gctacggtcg aattgctgcg cagcgcggtg ccgctggtgc aggagcggct ggtgctgctg
      841 agcgaggcat tgcccaagct cgcgtacctg ttcaccgccg atgccgatct gatgatcgac
      901 ccggaggcgc tggcgaaggc tggtgcggac gcgtccgcgg tggtggctgc ggcgaccacg
      961 gcgctgagcg ggctggggga gtggaaccac gagaccattc aggctgcgct gcgcgaggcg
     1021 ctggtcgacg ggctcgggct gaagccgaag ttcgcgttcg ggccggtgcg gctggccatc
     1081 accgggtcga atgtctcacc gccgctgttc gagtcgatgg aattgctggg acgggaatcg
     1141 tcgctggcgc ggctggtgaa gctgcagacc ctgctgtgag cgaggtcgtc gcgatcttcg
     1201 gcgggcgtag cgagatcggg ctggcgatcg ccacccggct ggctcccggc gccacgattg
     1261 tgctcgcggc gcgtcctggc ggtttgggtg ccgctttggc tgcctgccgg gacgccgggg
     1321 cagcccgcgt ggaaacggtc gagttcgatg ccgacgatct ggacgcccag gctggcgtgg
     1381 tggccgagat cgagggccgg gttggcccga tcgatgtcgc ggtgctggcc ttcggcgtgc
     1441 tcggcaacca ggcgttggcc gagcaggacc cgctggccgc cgccgcggta ctgaacaccg
     1501 atttcgtggc ccaggctgcg ctgctcaccg tgctggccga acgcatgcgg acacgggggc
     1561 gaggcacctt ggtcgcgttc tcctcggtgg caggcgtgcg ggtgcggcgc gcgaactacg
     1621 tctacggctc agcgaaggct gggctggacg ggttcgcgtc cgggttggcg gacgcgttgc
     1681 acggcagcgg agtgcacctg atcatcgccc ggcctggctt cgtgatcggc cggatgaccg
     1741 agggcatgga gccggctccg ttctcgtcca cccccgagca ggtggccgac gctgtggtgc
     1801 gccggattcg ctcgggccga ggtgtggacc tgtggatccc gtggcaattg cgcggcatgt
     1861 tcgcgatcgc ccgcctggtt ccccgtccgc tctggcggcg gatgccccgc tgaagtcgtc
     1921 cctcacgcct cccgccctag tcggaccctc caactttgct ccctttcggg agcaaagttg
     1981 agtcggacgg acgtttgggg ctggtcgaac tggtcgagac ttcggcgatc agaagttgaa
     2041 tgggatggat ggacgcgccg acgaccgacg ggcggaccga cccgcccggg acgcggggtc
     2101 aggctcagtg gccggactcg gcggcctcca actcgtcggg cagggtgggc aggccggcgg
     2161 gggcgcgtcc gctggcgaag cggatcaaca ggacgccgaa gatgatcagg acgcccccca
     2221 agatctgaat cgcgctcggc acctcgccca gcacgacggc ggccagcagg gccgcgaaga
     2281 gcacctccga caggctcacg aacgaggcca gccgctcgcc catcaacgag atcccggcca
     2341 ctcccagcgc ataggccagc gcggtcgccg agaccaccac cacgccgagc ggcacccacc
     2401 atgccaccga caacccgagc aactgaaccg gcacgaacgc cactgagtag gggagcagtc
     2461 ccaccaggag agccagcccg agcaccacgg caccggcggc caggccgaac gctgccaacg
     2521 cgatcggggg cagcgtggtc ggccgggcgg acaacacgaa gtaggcggcg gcaccgatca
     2581 tcgcgccgaa accgaacagc acgcccagag cgtccggggt ggcgccggtc agatcgagaa
     2641 cgcacaggag cccgagcacg gcggccagcg tcccgctcag caccagccgg gacggtgcgt
     2701 gccgtgtccg cacccaggcc agcagcacca acgccaccgg cgccatgtac tcgatcagca
     2761 gcgcgatgct cacgctcatc cgcgagacgg cagcgaaata catcagttgc ggagtggcca
     2821 cggccagcac accgaacgcg agcacggccc gccattgcgc caacaggtcg cgcgtccgac
     2881 cccggaccaa ccacagtccc accggcacca tcaagaccgc ggcggcagca acccgccaga
     2941 acaccgcagc cccgggcgtc cagccgatgt cgaacagcgg acggacgaac ggtccggacg
     3001 tggcgaaggc caatgaagcc agcagcccca gggtcacgcc aaggcctagc cgggtaggct
     3061 gattcggcgc ttcgggacta ctgctggggc tggtcacccg cctaatcctg ccgcagccgg
     3121 gagatttggc atctattggc ccgccatgaa agaatcgaca ggttgctccg aaccggacgc
     3181 ctgcgtggcg gccggcccgg atgtctggcg ctcgggtgcc gggccctacc acgaccaatc
     3241 aaagttcggt catgtgcggc ccagaatcgg tccgacacgc ccgaccgcgg gggtcggagc
     3301 agcagctcga tcggaagatg caccgggcga gcaacgacga acagagaagg acgaaccagt
     3361 ggcgggacag aagatccgga ttcgactccg ggcttacgac cacgaggtca ttgactcctc
     3421 ggcgaagaag atcgtcgaca ccgtgacccg taccggcgcc aaggtcgcag ggcccgtgcc
     3481 cttgccgacc gagaagaacg tgttctgcgt gatccgttct ccgcacaagt acaaggacag
     3541 ccgcgagcac ttcgagatgc ggacgcacaa gcggctgatc gacattctcg acccgacgcc
     3601 gaagaccgtc gactcgctga tgcggctcga ccttccggcc ggtgtcgaca tcgagatcaa
     3661 gcttccgtga ggtcggacga gatgagcgaa cgcaacgtaa agggactgct gggcaccaag
     3721 ctcggcatga cccaggtttg ggacgagaac aaccgcgtcg tccccgtcac cgtgatccag
     3781 gccggcccgt gcgtggtcac ccaggtccgc acccccgatg ccgacggcta ctccgccgtc
     3841 cagctgggct tcggtgcggt caaggccaag aaggtcacca agccggctgc cggccacttc
     3901 gagaaggccg gcgtcacccc ccgcaagcac ctcgtcgagc tgcgcaccgg ggacgccagc
     3961 acctacaccc tcggccagga gctcaccgcc gacgtgttcg acgcggctga gatcatcgac
     4021 gtcaccggcg tcaccaaggg caagggcacc gcgggcgtga tgaagcgaca cggcttccac
     4081 ggtctgcgcg cctcccacgg tgtgcaccgc aagcaccgct cgccgggctc gatcggcgcc
     4141 tgcgccaccc cgggccatgt cttcaagggc acccggatgg ccggccgcat gggtgtcgag
     4201 aaggtgaccg tgcagaacct gaccatccac gccgtcgacg ccgagcgcgg gctgatcctg
     4261 gtcaagggct ccgtgcccgg ccccaagggc gccctcgtcg tgctgcggac ggccgccaag
     4321 aaggacggag ccaaggcatg agcgagacca ggaccgtgaa ggtgctgggc ggcaagaagt
     4381 ccgccaccgc tgagctgccc gccgagctgt tcgatgtgca gaccaacatc ccgctgatcc
     4441 accaggtcgt ggtggcccag caggccgccg cccgccaggg cacccactcc accaagaccc
     4501 gcggtgaggt cgccggtggt ggcgccaagc cgtggcgcca gaagggcacc ggccgcgccc
     4561 gccagggttc gcggcgcgcg ccgcagtgga ccggcggtgg cgtcgtgcac ggcccgcagc
     4621 cgcgcagcta cgcccagcgc accccgaaga agatgatcgc cgccgccctg cgtggcgcgc
     4681 tctccgatcg ggcccgcgac ggccgcgtcc acgtggtcga cagcatcgtg accggtgaga
     4741 ccccgtccac gaaggctgcg ctgaaggcac tggccgcgat cggcggcagc aagctgctcg
     4801 tcgtgctcga ccgcgatgag gacgtggcct ggctgagcct gcgcaacgtc gccagcgtgc
     4861 acgcgctggc cgtcgaccag ctcaacgcct acgacgtgct gctcagcgac gacgtggtct
     4921 tcaccaccgc cgcgctggct gtcttcgccg gcgccccgag cgcggccgag gccgccatcg
     4981 agaccgagga ggacgccaag tgagcgacct gaagatccgc gaccaccgcg acatcctgct
     5041 ggcccccgtc gtcagcgaga agagctacgg cctgctggac gagaacaagt acacgttcct
     5101 cgtctccccg agcgccaaca agaccgagat caagatcgcg gtcgaggcgg tgttcggcgt
     5161 gaaggtcacc ggcgtgaaca ccagcaaccg gcagggcaag cgcaagcgca ccaaggccgg
     5221 ctggggcaag cgtcccgaca ccaagcgggc cgtggtgagt gtcgccgagg gccagcgcat
     5281 cgacatcttc tccgggccac tggcctgagg ctgagccgag aataggaaaa cgaactcatg
     5341 ggtatccgta agtacaagcc gaccaccccg ggccgtcgtg gctcgagcgt ggccgacttc
     5401 gtcgaactga ctcgctccac cccggagaag tccctgctgg ctcccaagcc gaagacgggc
     5461 ggacggaaca actccggccg catcaccacc cggcacatcg gtggcgggca caagcaggcc
     5521 taccgcctga tcgacttccg tcgctacgac aaggacgggg tgccggccaa ggtcgcacac
     5581 atcgagtacg acccgaaccg caccgcgcgg atcgcgctgc tgcactacgc ggacggcgag
     5641 aagcgctaca tcatcgcccc caacggtctc gctcagggcg ccctggtcga agccggcgaa
     5701 ggcgcggaca tcaagcccgg caacaacctg ccgctgcgca acatcccggt cggcaccacg
     5761 gtgcacgcgg tggagctgcg tcccggcggt ggcgccaaga tgggccgctc cgccggcgcc
     5821 aagatccagc tggtcgcccg cgagggcaag atggccacgc tgcggctccc gtccggtgaa
     5881 atgcggctgg tggacgtgcg ctgccgcgcc acgatcggcg aggtcggcaa cgccgagcag
     5941 tcgaacatca actggggcaa ggccggccgt agccgctgga agggcgtccg cccgaccgtc
     6001 cgcggtgttg cgatgaaccc gatcgaccac ccgcacggcg gtggcgaggg caagacctcc
     6061 ggtggtcgcc acccggtcac cccgtggggc aagcccgagg gccgcacccg cgataagaac
     6121 aaggcgagct ctcgcctgat cattcgtcgc cggaagtccg gcaagaagcg ctgattggac
     6181 aaggtgagat aagcaaatgc cacgcagcct gaagaagggt cccttcgtcg atgaccactt
     6241 ggtcaagaag gtcgacgcac agaacgagaa gggcaccaag caggtcatca agacctggtc
     6301 ccggcgctcc atgatcaccc cggacatgat cggtcacacg atcgcggtgc acgacggccg
     6361 caagcatgtg ccggtgttcg tgaccgactc gatgatcggc cacaagctgg gcgagttcgc
     6421 cccgacgcgc acctaccgcg ggcacgtcaa ggaagacaag aagtcgcgcc gccgctgacc
     6481 cggcgagaag aggaattggt tcaagtgagc aacaaagagc gacccacccg tcgcgcagcc
     6541 ctgctcgggg atcgtcctgg ttcgtacgcg attgcgcgtc acgtccggat gagcccgatg
     6601 aaggtacgcc gggtcgtcga cctggttcgt ggaatgccgg tgaccgacgc cctggacgtg
     6661 ctgaagttcg caccccaggc tgcgtccgag cccgtgtaca aggtggtggc cagcgcggcc
     6721 gccaacgctg agagcaccga gggcctccgt cgcgaggaac tcgtcgtctc cgcggcgttc
     6781 gtggacgagg gcatgaccct gcgccgcatc cgcccgcggg cgaagggttc ggccagccgg
     6841 atcctgaagc gcgcgagcca catcaccgtc gtcgtcgagc cgaagaccga gaagggagtc
     6901 tgagcatggg ccagaagatc aacccgcatg gcttccgcct cggaatcacc accgaccaca
     6961 agacccgttg gtacgcggac aagaactact ccgacctggt ggccgaggat gtcaagatcc
     7021 gtcgctacct tgccaagacc cttgagcggg ccggcatctc cagcgtggag atcgagcgcc
     7081 gcagcgagcg ggtgaccatc ttcctgtacg ccgcgcgccc gggcatcgtc atcggccgta
     7141 acggtgccga ggccgagcgc gtccgcgccg agctggagaa gctcaccggc aagcaggtgc
     7201 agctgaacat cctcgaggtg aagaaccccg agaccgatgc gcagctggtc gcccagggcg
     7261 tggccgagca gctgggtgcc cgcgtggcgt tccgccgcgc gatgcgcaag gcgcagcaga
     7321 gcgcgatgcg cgctggtgcg ctcggcatcc ggatccagtg ctccggtcgt ctgggtggcg
     7381 ccgaaatgag ccgttcggag ttctaccgcg agggacgcgt gccgctgcac accctgcgcg
     7441 cggacatcga ctacggcttc tacgaggccc gcaccacctt cggccggatc ggcgtgaagg
     7501 tgtggatcta caagggcgac gtctccggca atcgcgccga gcgcgccgcc cagaaggctg
     7561 cccggcaggc tgccggcggc cagcgccgtc ctgcccgtgg cccgcgtcgc gatggcggcg
     7621 ccggtgcggg tcgtggcgag cgtaccgagc gtggcggacg ccgtcgcgcc gagggtgtcg
     7681 ccgaaggcgc tgctgcgccg gaggccgtcg agagcaacgc aggagcctga ccatgttgat
     7741 tccccgcaga ctcaagcacc gcaagcagca ccaccccaag cggagtggtg cggccaaggg
     7801 cggtaccaag ctggcattcg gtgactacgg catccaggcg ttggagtcct cgtacctgac
     7861 caaccgtcag atcgagagcg cccgtatcgc gatgacccgc cacatcaagc gtggtggcaa
     7921 ggtgtggatc aacgtctacc ccgaccgtcc gctgaccaag aagccggccg agacccggat
     7981 gggttccggc aagggctctc cggaatggtg ggtcgccaac atcaagcccg gacgcgtgct
     8041 cttcgagctg tccggcgtcg gcgaggacgt ggcccgcgag gccatgcgcc tggccatcca
     8101 caagctgccg ttcaaggctc gcttcatcaa gcgggaagca ggtgacatct gatggctacg
     8161 tcagttactg ccaacgagct tcgcggcctg agccgtgatc agctcaacgc gaaggtcgtc
     8221 gagctgaagg aggagctgtt cgggctgcgg ttcgccgccg ccaccggtca gctcgaatcc
     8281 cacggccggc tgcgcgaggc ccgcaaggac atcgcgcgga tctacaccgt gctgcaggag
     8341 cgcaacctcg gcatcgtgcc cgacccggat gaggacaagt gagtcagatg agcgataacg
     8401 agaagcgcgg cgcgcgcaag gtccgtgagg gcatcgtcgt ttcggacaag atggacaaga
     8461 ccgtggtcgt ggccatcgag cagcggcgca agcacccgct gtacggcaag gtcatgacct
     8521 cctcggagcg cctgaaggcg catgacgagg ccaatgaggc cggtgtcggt gaccgtgtgc
     8581 gggtgatgga gacccggccg ctgagcgcta ccaagcgctg gcgtctggtc gaaatcctcg
     8641 aaaaggcgaa gtgaccagga gagatagcag atgatccagc aggagacgcg actgaaggtc
     8701 gccgacaaca ccggtgcgaa ggaactcctt tgcatccgcg tgctgggtgg ctccaagcgt
     8761 cgctacgccg gccttggcga caagatcgtc gccacggtca aggacgccat ccccggtggc
     8821 aatgtgaaga agggcgaggt cgtcaaggcg gtcatcgtcc gtacggtgaa ggagacccgt
     8881 cgtccggacg gttcctacat caagttcgac gagaatgccg ccgtgatcct gaagaacgac
     8941 ggggagcccc gcggcacccg catcttcggt ccggtcggcc gtgagctgcg cgagaagaag
     9001 ttcatgcgga tcatctcgct cgcgccggag gtgttgtgag catggcgaac acgctgcatg
     9061 tgaagaaggg tgaccgggtc aaggtcatcg ccggtaagga caagggccgc atcggcgaga
     9121 tcatctcggt cgacccgcag gccggacggg tgatcgtcca gggcgtgaac ctggtcaaga
     9181 agcaccgccg ggagaccccg aacaactcca cccgcggcaa caacgcgggc ggcatcatca
     9241 ccaccgaggc ccccatcgac gcgtccaacg tgcagttggt tgtgaaggtg gacggcaagg
     9301 acgtgaccac tcgggtgggc tacgaacggc aggttgtgac caagcgtcgc gccgacggca
     9361 cggagtacac cgctgagcgg agcgtccgga tcgctcgcaa gaccgggaag gaaatctgat
     9421 ggccgcgacc gcgaccaagg tattgccaag gcttcaggag aagtaccgct ccgagatcgt
     9481 cccggcgctg aacactgagt tcggctacga gaacgtcatg cagatccccg gtctggtgaa
     9541 gatcgtggtg aacatgggtg tcggtgaggc cgcgcgcgac tcgaaggtga tcgacgcggc
     9601 catccgcgac ctcaccgcga tcaccggcca gaagccgacc gtgaccaagg cccgcaagtc
     9661 gatcgcgcag ttcaagctgc gcgagggcat gccgatcggc tgccacgtca ccctccgcgg
     9721 tgaccgcatg tgggagttcg ccgaccggct gctcagtctg gccctgcccc ggatccgcga
     9781 cttccgtggc ctctcggccc ggcagttcga cggcaagggc aactacacct tcggcctgac
     9841 cgagcaggtg atgttccacg agatcgacca ggaccgcatc gacaagctgc gcggcatgga
     9901 catcacgttc gtcaccacgg ccaagaccga cgacgagggc cgcgccctac tgaaggcgct
     9961 cggcttcccg ttcaaggccc cggccgacga ggtcgccaag gtcgtcaaga agaagggccc
    10021 ggcgttcacg ggcaagaaga agaggtgaac tgaaccatgg cgaaaaccgc tctgaaggtg
    10081 aagcaggctc gcaagccgaa gttcgccgtc cgcgcctaca cccgctgcaa caagtgcggc
    10141 cggcccaagg ccgtctaccg caagttcggc ctgtgccgga tctgcgtccg ggaacttgcc
    10201 cacgcgggcc agctccccgg cgtcatcaag tcctcctggt gatctgatca ccgcgagcta
    10261 actacaaccc gctgaaggtc cgcgacaccg cggaaaccat ggcgagaaag agacaccaca
    10321 cgtcatgacc atgaccgatc cgatcgcgga catgctgacc cggctgcgga acgccaatca
    10381 ggcgtaccac gagacgacca gcatgccgca cagcaagatc aaggtgggga tcgccgagat
    10441 cctcaaggct cagggctaca tttccggctt cgaggtgctt gagccgggcg ccggcgaagt
    10501 cggcaagacc ctgaagctca ccctcaagta cggccagaac cgcgagcggt cgatcgctgg
    10561 cgtgcgccgc atctccaagc cgggcctgcg ggtctacgcc aagtcgaccg cgctgcccaa
    10621 ggtgctgggc ggcatgggga tcgccatcat ttccacctcc caaggtctgc tgaccgacaa
    10681 ggaggccaac actcgttgcg tcggcgggga agtcctcgcc tacgtctggt aacggaagga
    10741 gctgaacaga tgtcacgaat tggcaggctc cccattgcta tcccagcggg agtggacgtc
    10801 accctggacg gtcagaccgt gtcggtgaag ggccccaagg gcgctctcgc tcacaccgtc
    10861 aaagagccga tcaaggtcgg gcgcaacgac gaaggtcagc tcgaggtcac ccggccgaac
    10921 gacgagcgcg aaagccgctc gctgcacggc ctgacccgga ccctggtctc caacatggtc
    10981 atcggcgtga ccgacggcta tgagaagaag cttgagatcg tgggcgtcgg ctaccgggtg
    11041 atcttcaaga gccccgagca gctcgagttc aacctcggct tctcccaccc ggtcgtcgtc
    11101 aacgcgccag ccggcatcac cttctcggtg gagagcaaca ccaagttcac cgtgtacggg
    11161 atcgataagc aggccgttgg tgaggttgcc gccaacatcc gcaagatccg caagccggag
    11221 ccctacaagg gcaagggcgt gcgctacgcc ggcgagcggg ttcgccgcaa ggttggaaag
    11281 gcgggtaagt gatggctatc tcactctcga cgaacaaggg catggccgat cgccaggctg
    11341 cgcgcctgcg gcgtcaggct cgtggtcgca agaagatcgc cgggtcgccg gagcgtccgc
    11401 gcctggtggt cttccgctcc agccggcaca tctccgctca ggtgatcgac gacgcggccg
    11461 gggtcaccct ggcctcggcg tcctcgctgg agctgcgcgg tgcggacggc gacaagtcgg
    11521 ccaaggccaa agaggtcggc aagctggttg ccgagcgcgc gaagaaggcc ggcgtggaga
    11581 acgtcgtctt cgaccgggcc ggcaacaagt accacggacg gatcgcggcg ctggctgaca
    11641 gcgcccgcga agccggcctc ggattctgac ggatcgaaag gtaagcaaag cgatgaatgg
    11701 aacccagcgc cgcggtggcg gtgccggcac cggtaccggt ggtgagcagc gtcgcggcgg
    11761 ccgagaagac cgtcgtggcc gtgatgccgc cgccgagaag gataagagca actacctcga
    11821 gcgggtagtc gcgatcaatc gagtggccaa ggtcgtcaag ggtggtcgtc ggttcagctt
    11881 caccgcgctg gtcgtggtgg gcgacggcga cggcatggtt ggtgtgggct acggcaaggc
    11941 caaggaggtg cccgcggcaa tcgccaaggg tgtcgaggag gccaagaagc agttcttccg
    12001 cgtgccccgc atccagggca cgatcccgca cccggttcag ggcgagaagg ctgccggtgt
    12061 cgtgatgctg cgtccggctt cgccgggtac cggcgtcatc gccggcggtt cggctcgcgc
    12121 cgtgctggaa tgcgcgggcc tgcgcgacgt gttggccaag tctcttggtt cggacaacgc
    12181 gatcaacgtg gtgcatgcca cggtagccgc gctgcagagc ctggaggagc cggaggcggt
    12241 ggccaagcgc cgcggtcttc cggtggcgga cgtcaccccg gcggcgatcc tgcgggctcg
    12301 cgaagagggg gcgcacgcat gagccggttg aaggtgaccc agatcaagtc tgggatcagt
    12361 ggaaagcaga atcagcgcga caccctgcgt tcgctcgggt tgaagcgcat cggcgacgtc
    12421 gtggtgatcg aggatcgccc cgaactggtc gggatggtca agaccattcc gcacctcgtc
    12481 tccgtcgagg aggttgagtg acatggcact gaaggttcat cacctgcgtc cggccccggg
    12541 cgcgaagacc gagaagaccc gcgtcggccg cggtgaaggg tccaagggca agaccgcagg
    12601 tcgaggcacc aagggcaccg gcgcacggaa gaacaccccg gagaacttcg agggtggcca
    12661 gatgccgctg cacatgcggc tgcccaagct gcgtggcttc aacaacccgt tccgagttga
    12721 gtaccaggtg gtcaacgtgg cccggatcgc cgagctgttc ccggccggtg gcgaggtgac
    12781 tcccgccgaa ctggtggcca agggcgccgt ccgcgacggt cagctggtga aggtgctcgg
    12841 caacggcgac ctcacggtgg ccgtccaggt gagcgcgcac gcgttctctg ccagcgccaa
    12901 ggagaagatc gcgaaggccg gcggttcggt cactgaactg tagtcagcct tgtcagtacg
    12961 gcccccggca gcagccgggg gccgtactgc gttccggtgc ggatcgggga gtgcgcccgg
    13021 accgggtcgg tcgccggttt ggcggctctg attcgagcgt gccaaactgg tgctgaaccc
    13081 agacttggga tcccggacga gggagccgta cccggaacgc gacaagacgg ccttcgaaag
    13141 gttgatcgat gtcctggttg gtactggtgt tgtccggaat cctcgaatcg gtgtgggcca
    13201 ttgcgctcgg ccggtcggag ggtttctccc gtccgctgcc cacggtgatc ttcggggttg
    13261 ccctgctgtt cagcatgggc ggactggcct atgcgatgcg cgagctgccc accgggacgg
    13321 cctatgccgt gtgggtcgga atcggtgcca cgatcacggt cgcctacggc atgttcaccg
    13381 gcgaggagcc ggtctcggtg ctgcgcatcc tgctgctggg cgtgatcgtc gcgggcgtca
    13441 tcggactgaa gctgctgcat tgatcaaacc gcgggccccg ctgcggcgcc cgaatacgtg
    13501 atccggcgta cggcttgata gagtttgccg ggttgtcccc ccgcggttcg ccgcaggcat
    13561 tcgggtgctg cgctcgtgca ggtcccatcg cagatgtaag gagtaggtcc acttcatgct
    13621 ttccgccttc gcgaacgcgc tacggacgcc tgacctgcgc aggaagattc tcttcaccct
    13681 gatgatcctc gcggtgttcc gcctcggctc ggtgatcccg accccgaacg tgaacattgt
    13741 gaacgtcgac cagtgccgca cgctggccac cagcggtgac tcggccggcc tgtactcgat
    13801 gatccagctg ttctccggcg gtgcgctgct gcagctggcg attttcgcgc tgggcatcat
    13861 gccgtacatc accgccagca tcatcctgca gctgctcacc gtggtgattc cgcggttgga
    13921 ggcgctgaag aaggaaggcg cggccggcac cgccaagatc acccagtaca cccggtacct
    13981 gacgctggtg ctcgcagtgc tgaactcgac cgcgttcgtc accctcgcgg tcaacggtgg
    14041 cctattccag ggctgctcgc tcgacctggt ctacgacaag ggcctgttcg cgatcatcac
    14101 catgatcctg accatgaccg caggcaccgg cgtgatcatg tggctgggcg agctgatcac
    14161 cgaccggggc gtcggcaacg gcatgtccgt gatgatcttc acccagatcg ccgcgcagtt
    14221 ccccgcgtcc ctgtggtcgg tcaaggagag ccgcgagggc gcccagggct ggctgctgtt
    14281 catcctggtg ctggccgttg gcctcgcggt gatggccggc gtcatcttca tggagcaggc
    14341 gcagcggcgc attcccgtcc agtacgccaa gcggatggtc ggtcggcggt tgctcggcgg
    14401 gaccaccacc tatatcccga tcaaggtgaa ccaggccggc gtcatcccgg tgatcttcgc
    14461 ctcttcgatg ctctacctgc cggtgctcta cgcgcagttc cagccgggca cggtggacaa
    14521 tccgaacccg gtgtcggcct ggatctccgc gaacctcgcc caaggcagct gggtgtacga
    14581 gttgctctac ttcgtcctga tcatagcttt cgcctacttc tacgtttcga tcaccttcga
    14641 cccggtcgag gtcagcgaca acatgaagaa gtacggcggc ttcatccccg gcgtccgcgc
    14701 cggccgtccc accgagcgct acctggcaca cgtgctgtcg cggctgaccg cgcccggctc
    14761 gctgtacctg gccctggtcg cactgatccc ggcgatcgcg ttcgtcctgc tgggcacgca
    14821 caacaagttc cccttcggcg gtacctcgat cctgatcatc gtcggagtcg gtctggacac
    14881 tgtgaagcag atccagagcc aattgcagca acgaaactat gaaggattcc tcagatgaga
    14941 ctgctcatca tgggcccccc cggagtgggc aagggcaccc aggccaccgg tatcgccgcg
    15001 cactacggtg tgccggccat ctcgaccgga gacatcttcc gggccaacgt caagaaccag
    15061 acgccgctcg gccaggaggt ctcgcggatc atggctgacg gcggctacgt gccggacgtg
    15121 atcaccaacg cgatcgtggc cgaccggctc accgaggccg acgcggccaa cggctggctg
    15181 ctcgacggtt acccccgcac gatcggccag gtcgaagccc tcgacgaagc gcttggcgag
    15241 ggccccggca tcgacgccgt gatctcgctg gtcgccgaca ccgatgtgct ggtcgatcgg
    15301 ctgctgaagc gggccgagct cgaaggccgc gccgacgaca acgccgagac catccgcaac
    15361 cgcatggtga tctacaccga ggccaccgac ccgctgctgc agacctaccg cgaccgtgac
    15421 ctgctggtcg aggtcgacgg catcggcggc atcgaagagg tcggccagcg gatcaccgct
    15481 gcgctcgacg ccaagctctc ctgagcttca cggctggcta ggacggacgt gcgtcgccga
    15541 cccggaattg agctgaagtc tgcggaccag attcggcgga tgcgccgggc cggcctggtg
    15601 gtcggccgga cgctggccgc ggttcgcgcg gccgcggcgc ctggcgtcac caccgcggag
    15661 ttggacgcga tcgcgcgcga gcagatcgcg gctgcgggag cgctgtcgtc attcctgggc
    15721 tacggcggcg ggttcggcct gccgccgttc ccggccgtca cctgcatctc ggtcaactcc
    15781 gagatcgtgc acggcatccc gggggagcgg gtgttggcgt ccggagatct cgtcagcgtt
    15841 gacttcgggg tcagcattga cggctggcac ggtgattcgg ccgtcacctt cggggttggc
    15901 gagctgtcac ccgaagcgtc cgcgctgagc gaggcgaccc gggaagcaat gtgggccggg
    15961 atcgcgtccg cgcggatcgg cggccggatc ggcgacatcg ccaatgccat cgagaccagc
    16021 atccacagtc attcggtcgg gtacgggatc gtgtccgagt acaccggtca cggcatcggc
    16081 accgccatgc accagccgcc ggacgtgccc aacctcgggc gcgccggacg tggcgagctt
    16141 ctcgccaaag gcatctgcct ggccatcgag ccgatggtca cgctgggctc ggccaacacg
    16201 gcgaccctgg aagacgagtg gaccgtggtg accagggacg gctccgtggc tgcgcactgg
    16261 gagcacacga tcgcactcac accggccggg gtatgggtgc tgaccgcagc ggacggcggc
    16321 gaagccgaac tgaccgcgcg tggggtgaag ttcggcccgc tggcggactg ataaagcgcg
    16381 tccatctggt cgggttgggg tgcgatcgcc gctcagccaa gcgcagccgt cattggctgg
    16441 tacggatcac gtcggatcgt tccggaagag cggtatgtcg ccgcatcggc gtcccacaga
    16501 ggcgaattgc cccggctcca gaaccggcgc agatcgacaa cccggcacag atcgcgtccg
    16561 ctgcacgccg atcgcgacac tcaccgcgtg tcgccgccaa tcggacgcga ttcgcgccgc
    16621 ggccgatcga cgagcggatc aacctagctt tgcttgccag atgccgaagt tggtgtatcg
    16681 gcggtagccg agcgcggtgt tgatggcgag catccactcg ttctcgcccg cgttccaggt
    16741 gtcgacgtgg actgcgtccg gctgggtctg ggcgaggagc tgcagattcg ccaccttcac
    16801 caggagtccg agacgatgcc cacgatgggc tcgatcgacg gtcgtggtgc cctgctcaag
    16861 tcgctgcgga tgcgcgttcg gcaggtgcag gtcggtcaag gcgaccacat ctccgctggc
    16921 cacctcgacc gcgacggtgg tcagcagtcg tgaagtgcga tggacccgct cttccatctg
    16981 ctggacgcgc gcggcgtccc agatctccgg ctgccagtca acgtcaccga gcggtgcgtc
    17041 ggtggagagc gcggcgtgca tccgggccac gccgtcgcgg agctcggctg gtgtcgggcc
    17101 ggcccaggag atcgcccggt actccgggga cgccttggct tcggcttcgg ccagcaactg
    17161 agccagcagt tccggggcca gcggcagcag caaccgggat tgccgctcga cctgcgccag
    17221 cgagtaacca gcggcgagtg cgaagcggga cacgtggtcg agcggcacgt ggccgccgcc
    17281 gtccggaccg acgagatcgt ccgcacgcgc cggggcgccg ggggagtgct gcgaccagcc
    17341 gacgatggtg tcgcgaccct cgtcgacagc gatccgccgc acctcgcggg cgagggctgc
    17401 gccgatgccc gcgcgccgct ggtcggcccg aaccatgatc gagacgttgt ccagcaggtg
    17461 caggttgtcc gtggtgggca ggtagatctc ggtgatcccg agcactttga ccggctcggc
    17521 gggcacaacc ggcaggccgt agcgaccgat cagtgcgtcc gccggtgcgg gccctggcaa
    17581 cgccagcagc agcgccttgc ggccgtgcac ctgatcggcg tgccaggcct gcacctgatc
    17641 ggtgggggtg gagttgtcct cgtcgtacat ctcgacgctg atcgcggcgt ccagctcgtg
    17701 gtagcccgcg tagacccagt cgggtccggg cgctgccgtg cagatgtgga actcgcgcat
    17761 tcgcgaagga taaccggtgt gctgtgcgcc gccacagcgc gggggacggt caagcgctag
    17821 cctttggctc gtgtccacca ccgccaagcg tcgcgtcctg gccggcctgg tcgacttggg
    17881 gctgatgctg ctttgggtag ctgtggtggc cgcggtcggc gtcccgctct acctcaccgg
    17941 agtgaccgcc acggtcgggc cgttggcgca gaacctgatc gggctgctgt gcgtcgtcgt
    18001 gccggtcacg ctcggactga ccctcgccga atgcggacgc tatgaagcca ccccagggaa
    18061 gctgaagttc ggcctccggg tgcgtaccga caccggcgac cgggtcacct ggccgcgctc
    18121 gctgctgcgc aatctactca agctgggact gccctggacg ctcggtcacg ccggggcggt
    18181 cgcggtcgcg tacggcggcg gctgggacgc gcaactcggt gcggtgctca gcctgctggt
    18241 gccggtggcg tacctcgtct cgctcttcgc gggcagcgga cggacgatct acgaccacct
    18301 cgccggcacc acggtgatcg agaccgcgcc gggacggcgg ttcgcggact gattcgggcc
    18361 gcgcgggcct cagccggcgc gcagggcgtt caccggttcc acccgggacg ctcgatgtgc
    18421 ggggaaccag cccgccagca ggcctacgac tgcgccgagc gcggccgccg caacggccag
    18481 ccacgggtcg atgatcgggg tccactgctg gatcagcgcc acacccacga ccgcgaacac
    18541 cccaagggat gcgccgatca ggccgccgag caggccgatc agcactgatt cgaggacgaa
    18601 ttgcgcggcg atctgccgtc cggtcgcgcc gacggcgcgg cgcaggccga tctcgccgac
    18661 ccgttccagc acggacagca tggtcacgtt ggcgatgccg agcccaccgg ccagcagggc
    18721 aactacgccg agtaccagga agacgatgtt gacgtcggct tgcacattgg tgctcagctc
    18781 actgtgtccg gacggagccg cgacctcgat attctcgggt gcgtccggtg ccagcgcgat
    18841 cgccgcctgc ttgccgacct gcgggccggc gccgaccacg atcttcacct gcagttcacc
    18901 gggggcggtg acgccgaagt cctcgcggcc ggcacccatc ggaatgatca cggagtcctt
    18961 cagttcggcc ttgcgctgga agtcgtcggc gatgccgatc acggcgtagg ccaggccgga
    19021 gatgaagatg gacggctgcc ggtcgacccg gctcacgccg agccgctcgg cgacccgtac
    19081 gcccagcacc gcgacccggt cggcccgctg gtcgtgtccg gagtcgaagc agcgtccggt
    19141 gacaatatgg ccgctgagcg cgtccatcag tcccggggat gccgccacca ccgcgggcac
    19201 ggccgccgag gccgcagacg ggtcgttcac cggcaccgcg gtcaccgtgc cctcgcccag
    19261 acccacctcg ctgaacaacg cggcggactg caccccggcc agccggccga cccgctcgga
    19321 cgcgtcccac ggcaaccgga ccgtcgccac gcttgccccg ctgccggtgc gtgaggtctt
    19381 cggggtgatc accacctgcg tcgcggccgc cgcgtcgaac tggcgcgcga tctggccggc
    19441 cgcggtctgg gcgaaaccga tcgtcgcgat cagcgaaccg atgccaagca ccgtgcccag
    19501 gatcgtcatc accagccgtc ccggacgcga accgatgccg gcagtggcct ccatcagcag
    19561 gtcggcgaag ccgaaccgat ccgcccgtgc caccgggctg agcagggccg acgcgctgtt
    19621 cacagcggtc gcacccgcgt ccgccacccg gccgcggcgc agccaggatg gcaaccgggt
    19681 cagccacgag gggagggaga gccgcttcat caggccgcct cggtcagccg gccgtcgagg
    19741 atccggattc gacgtgttgc ccgcttcgcc accgcggcgt cgtgggtgat cacgaccagg
    19801 gtcagtccgt ccgcgtgcaa ctcctcgaac agcgacatca cctcgccgga cgtgccctgg
    19861 tcgagattgc cggttggttc gtcggcgagc agcagctgag gtccgctgac cactgcgcgg
    19921 gccaccgcca cccgctggcg ctcaccgccg gaaagcgtgc ccggcaggaa gttcactcga
    19981 tgggcgaggc ccacccggga cagcgcggtg cgagcgcggt cctcgcgttc ggcgcgcggc
    20041 accccgctgt acagcatcgg cagcagcacg ttgtcgagca ccgaacgatt cggcagcagg
    20101 tggaaggcct ggaacacgaa gcccacgacg cgcgcccgga cggccgcacg atcgtcctcg
    20161 gtcagatcgc gggtgggtgt gccggccagc cggtactcgc cgaccgaggg ccggtccagc
    20221 aggccgagga tgttcagcat ggtcgacttg cccgaaccgc tcggcccgac gatcgacaca
    20281 tagtcggcgg cggcgatgtt caggttcacg tccttcagcg cctgcacctc cggcggcccg
    20341 gggaaggcgc gggtcaggtc gaccaactcc accagcgggg ttgcggtcac gtacccacca
    20401 ccacaaggtc gtccttcacc agggagccgg cgaccggcgc gacctcgacg tatccggctg
    20461 cggccagtcc cgtccgcacc accaccaggc gggtcttggc ttgggccttg tcccgcgggt
    20521 cgccctcgac gacctcaacc cgcgcttcgc cgcccggacc agcggtgagc gcggccagcg
    20581 gcaccgaaag cacgtcaccg tcggaagagc cgaccgcgat cgtcagcttc acattgctgc
    20641 ctttcagctg ctcggtctgc tcggcggtga gcggatcggg ggtgaacccg atcgtcgagc
    20701 gatcgccctt gtcagcggcc tcgatggtcg cgatcacggc gcgatgctgg ttgccgtccg
    20761 gcagttcgaa gaacgcatcc ttgccgactt cgaggagctt ggcgtcggcg gccgagaccg
    20821 agccgctgag ttcgagcttg gccccggaca cgctcatcgc agcgccatcg aggatgtcgc
    20881 cacgctcgac cttcacctcg tccacgcgtc ggggcagctg ggtcaggtag agcacttcgc
    20941 cggccggcag gtagggcagg gctgccttct tggcgtccgc tagctgcttg gccgcatcct
    21001 tgcgttgctg cttggccgag ctcagtgctc cggcctccgc gctggtgtcg ccgcgcttca
    21061 acgcctcctt gcgacgcagc ttggcgagcg cgagctcgtc cttcagccgg gcgacctcaa
    21121 gcgcgtccgg gctgtcggcg gcctgagcag cctggagcgc ccggcgagcc tcggccacgg
    21181 cgttgtccgc ctccttgcgg gtgaccgcgg aggggccctt tctggcttcg gcgagagcct
    21241 tgcgcgctga agccagtccg ctgtcggccg agcgcagggc ttccctggcg gtgcggacgc
    21301 cctccgcagc ggtactgtcc accggagcgg tgtagccggc ccgcaggtac agggtgcgga
    21361 cggccttcgc agtgtcgttg tcgaacttgc ttccgctggt caccgagatg ccctggtcgg
    21421 cgagcgcctg cttgagctgc ttcacatccg gcccggacgt gcccacccgc agcgtgcggt
    21481 aggccggcag cttgccgggc aggacgaaca cgggccggcc ggccacttcc agcgcgatgt
    21541 caccggcgtc gagttcggcg ccagctttcg ggacctggcc ggtgaccacg gctgcgtccg
    21601 cgccctcggg ggcagcgatc ttcacctcgg tggagtcggc gtagcccacc tggccgcgca
    21661 tggtcacgtc gttggtcagg gttccgaacg cgaccggcac ggtgatgtag cccgcctccg
    21721 gcgcggcggt tcgggcggct gcgtcggctg gggagagcac gaagcggccc accaccaggc
    21781 cagccaccag gcaggcggtg gcgaccgctg cggtcaccca gagcgcccgg ttctcgcgga
    21841 agatgcgcag ccagcccgcg gcgcgtccat gtccaggact ggctgcggca gcggcttgtg
    21901 gatcgggcga gtcgggtgac tcagggtcgt cggcggcggc tgccagtgct tccccttggg
    21961 cgaccggctc ggcgtccgct gcccctggac gacgccgcgc cactagcgct ttccctggtc
    22021 agccgaggct ttgagtgcgt ccagttcggc cttgtgatcg gagatgaact ggtcctccag
    22081 tttgaactgg atagtcagct ggcgattgcg gtagtcggtc ttctcccggc agtcgaggtc
    22141 ggccagggcc agctcgacct ccttcttgcc gatctcggcg aaggcagggt ccttcagcgt
    22201 gccgaggttc ggatccatct ccgtttcctc gccaccgtcg gccgggtatg cctcctcgta
    22261 gtacttgttg accaggtcgt agatcgagtt cgaggcttcg gactgggtct tgaagccggt
    22321 gtggccggcc gtgtccatgc aggcgaccca ttcgctgtcc agcgccgaat actcggtgtt
    22381 ggtcggcagc ccctcgtaga acttctgcaa cgcgtcgatc aaaggcttga actccgcttt
    22441 ctcatagggt tgatcgccca tcacctcatg ttgggccgcg ccgtagcagc cctgcttggt
    22501 ccagtcccag gtctgtgcct cgtcggtggg ctccgcatcg gtcggctcgg cggttgcggc
    22561 ctgcccgccg tagagcgctt cgttgtaggc ctgctgctcg gcctcggtca ggctcgccag
    22621 gtactcctgg ttcgggtcga cctgctcggt accggtcggc tcctcgaccg gcgcatcgtc
    22681 cttgcccggg aagttcaccg agccgtagcc gtactgcttc acccactcgc gatcgtcggg
    22741 cttccaagga ttctcctctg tgccgccgac cgttaccgag gtggtggtgt tcggcttgta
    22801 ctcgaagcct tccttcttca tacattcggc gacgagttgc tcggtcttct tctgctgctc
    22861 gtcgtagtac gcctgctgct cttccttggt tgcgtccgga ccaggcccga gggagaggtt
    22921 ggcgaggtac ttcgtcaacg gtgaatcctc cgccttcagt gccgatggcg cgcccaggcc
    22981 gggtgcgccc gtgcagccgg ccagcgtgaa tgccgcgatt gcgattgctg aggtgaggcg
    23041 tcttggtcgt ggcatccgtg gcttctttct ccggcgaggg tggtcggaat tccacggtag
    23101 cggaccacgg tgggtgtatg gcgtcgtccc tagggatgat tcgtgctcgt ccgttgggcg
    23161 gatcaatccg tgctatttcg gcgaagcgcc ctttccggag ccttatttct gaagatctac
    23221 tgagaatcgt ggcgcgcaat ccgttttggg cggttctgga cgaagagggt cagaaccggg
    23281 ttgtttgatt ggttcatgga cgagcgattg cgggatctgc cggtgcgcga agcactggca
    23341 cggattcgcg ctgtcctgga tcgggtgaat cccgaccgca aggcgttggc gccgcaggac
    23401 cgtctgtttc ttgcgaccgc ggcccgaagc ttgaccggac gcttggaggc gctgggctcg
    23461 ctgttgctgg ccggagctga ccgtgccaac gcgtcggagc gttcggctgg gacgccgacc
    23521 tcgacgtggc tagcgttgga tcagaacctg accaaacgtg aagctgccgg cgcgttgcat
    23581 cgagctactg agctggctgc gcacccggcg ctgggggagg cggcggttgc tggacgggtg
    23641 ggcaccggtc aggtgcgggc gatcaacacc gtgcttagcg gactggctgc tcagctcaac
    23701 gaccagcagc aggcgcaggc cgaggagttg ctggtgggcc tggccgcctg catggattcg
    23761 gatgtgttgg gcaaggccgc cgcgcaggtg ttggcgaagg tcgccccggc cgatgctgac
    23821 gaggtactcg aaaccaagtt gcagcgtgag gccgaggctg cgcaccggcc cgcagtctgg
    23881 gccggccgcg aagcctgccc accgaccgcc gagcgacgcc gactgagctt cacggagcct
    23941 tcgtcaagct cagggatcgg tggccgcgcc gcgactggtc aagcttcttg gagacccgaa
    24001 gccgcgtgat ctcgacaggc tcgatccatc gaactcacgg ccgcccgacg agctcagtct
    24061 tcgcggctgg cgaggcgtgg cgccgaggcc tccggagcgc tgcgggtcgg cctgggacgg
    24121 gggatgggcc ggggccggcg atacgccttg ttggtcggac ggatgtcggc gccggtcagg
    24181 tcgctgtgca cccggtacgc gacgagggca ccggtcagca actgcgccca cgcgtccggt
    24241 ccgccgggcg accaggccga tgcagccagc acgcctggca ctccagtggt gagcttcggc
    24301 agtgacagca gcgtccgcca tgagtcccag cgcggtccgc cagagggcga gcggaccagg
    24361 cgcgcggcca cgcgaccgtc cgtccggttc tgggtcaggt ccacctgggc agccggatcg
    24421 agcgtgctga tcaccgcatt caggtaggtc gagccgaagg ctgtgtcaca gcgcagccca
    24481 cgcgcgtctc gtcgaattgc gctgaccgcc tccgtccgaa tctccacacc gcagccggcc
    24541 acccgctcca acagcaactc caccagccga ttcgcgggct gtggcgcacc cgcgccgtcc
    24601 accaaccgcc agcgtccgaa ggtgcgttcc acgctgagcc gcgcggcgtg ccatcccggg
    24661 aggcgccggg ggtcctgacc gagtcgggcg gcgacatcga gcaccatttc gctaagttcg
    24721 gccgtcggca gccggcccgc cagacgggcc aggctctccc ggggtcgcag tacctcgcgg
    24781 acctcagcgg tcagctgttc gagtccagtg aactccgcct ccaggccgag ccggcgctgc
    24841 acctgccaga cgtcgtccag gtgatcgaga aggtcacgcc aagccacggc ggccggctcg
    24901 ccgagggctt cgccgagcac cgtccactgc tcaccacgat cggacgggag cgccagcgtc
    24961 gcgccattgc tgaaccggta ctcgcgtgca ggtgccggcg cgaggccgag cccccgcttg
    25021 gccagttcgg catcgagggt gcggccggac ttgtagaaca ggtcccgcca cgcggccggc
    25081 agggtgaata cgtcatggtc gggctcgctc gccacctcga tccctgccgc ctgggcccaa
    25141 ccgccgctct ggcccagccc atcaacaccg ggttcgaacg acgcacccgg atcgaacacg
    25201 accacctgat gcccctcgcg ggaaagccgg gcggcggcgg cgagtccagc cagcccgctg
    25261 ccgacgatcc cgatcaagtt cgggccagcc aggcccggaa ggcaatgacg cccttgcggc
    25321 tctgggctat cagcagcacg gccagcgcga tcgtggcgaa cagcaacagt gcggcgatga
    25381 tcgcggcctg caccgggtag gcgatcgcga gtgagaccac gccgaccagg gcgacgtcgc
    25441 cggtcagcga ggcacccacg ttactgaacg gctccggcga agtgttcacc gccagccgga
    25501 cactcgattt ggcggcatga ctgattagtg cggtcacccc gcccagggcg ccgagcgtga
    25561 tcgggagcag ttcgccgctc gctccggcca gcagggcccc gatcagggcg ccggcgactg
    25621 gccggatgaa ggtggagatc ccgtcccacg cggagtcgag atagggcacc ttgtcggcga
    25681 tgaactcggc cgcggccagt acgcccatca cgatcagcac atcggtgcgc tggaacccgt
    25741 ccggtatctc cgagatgccc gcgaaccgtc cgagcacgcc cagcaggaag acggtcgcgt
    25801 aggcgttgat gccgctcgcc cagccgctgg cgaaggtgag tgcgacgacg tccatgccgg
    25861 aatggtagtc agcgttgccg cacggtggtg aggctgccgg acagatgttg ggccgcgtgg
    25921 agccctgcat cccactcagt tcgtcgcgca aacagctggt cgagtgaaac gaacaactgt
    25981 acgattgtct gaccaggcaa ggagttggca tgcggattga cggcgatggt gcgcgtgcag
    26041 atcggacgat catgcacgtc gacatggacg cgttctacgc ttcggtcgag ctgcggcgcc
    26101 gccctgagct gcgcgaggtg ccgatgtggg tgggtggcgc ggggcgcggg gtggtgctct
    26161 cggcgaatta tccggccaga gcccacggcg tgcggggtgg aatgtcctca accgcggctc
    26221 gaaggctgtg tccgcaggcg gtcagcctgc ctccggattt cgatgcctac accgaggtgt
    26281 cggacggggt cttcgcgatc ttcgaagcct tcaccgcccg ggtcgagacc gcttccatcg
    26341 atgaggccta cctggatgcg accggggccc tcaatcccgg cctgtcggcg tccgcgctgg
    26401 gtgagttgct gcgcgccacc gtccaggacg aacaggcgat cacgtgttca gtcggcatcg
    26461 ggcccaacaa gctggtggcc aagatggctt cgaacgcggc gaaaccggac gggcttctgg
    26521 aagtgccgcc ggaagcggtg gtcgctttcc tgcacccaca accggtggag catctggtcg
    26581 gagtgggtga gagcaccgcc gcacggcttc gccagctcgg catccatacg atcggcgagt
    26641 tggcgaaggc cgagcgcacc ggactgcgcc aggtgttcgg cccacgcgct ggaatgctgc
    26701 tttcggagtt ggcctggggt cgtgattcca gccgggtcgt ggcgcggccc ggggaacgcg
    26761 gtgtcggctg ccaggagacc ttcggccggg acaccgacga ctcgaacacg gtgcagaccg
    26821 agttgttgcg ggtctcggtg aaggtcgctt cgcgaatgcg ggccgccgag gtgctgggca
    26881 ccacggtgac gctcagcctg cggtacgccg acttcagcac cgtctctcgc tcgctgaccc
    26941 tggctgcgcc aaccgaccgg acagacgaga tccacggcgc cgcgctacgg ctgctggcca
    27001 ggctcgtccc acagcggacg cggatccggc gggtcggcgt ccgggtgacc ggcctcaagg
    27061 accgcgcccg ggtctatctg caacccaccc tggacgcgcc ggagcacggc tggcgtgagg
    27121 ccgaagttgc ggccgacaag gcgattagga agttcgggcc gaatgcggtg cggcgcgcgg
    27181 ccctgaccgg ccggcagcgc ccatgagttt gtgtgcctag tcgaacgggt ttcgtatcgg
    27241 cgtcaagcgg cctaaacttg gcatctcagg gccactagac accaggaggc gcccatggcg
    27301 ctctcggacg aagagcagcg actcctcgac cagatggagg ctgccctggc ggcagaagat
    27361 ccgaaactgg ccaacacgct gcgcggaagt acccggcaat ggcaacgtcg ccggctcatc
    27421 atctctggtc tgggcttcgt gcttggcgtc gtcgccctga tcggaggcat ggaactgcac
    27481 cccgtgatca gcgtgctcgg gttcctggtc atgctcgcct cggccgcggt cgcggtcacc
    27541 gcttggcagc gctccggcgc ggcagcggac ggtcacggcc aaggcggggc cggcagcgac
    27601 cgcgatttca tgggcaagat ggaggagcgc tggcgtcggc atcaggatga gagtggcctg
    27661 tagggctaag gctcctcacc acggccgcag cgaccgcggc caccaacgat ccaaccaagc
    27721 ggtgggctca gcccaccgct tcttcattgc cgcttcgatg cgttcgaccc gagcggtcag
    27781 ctcggccacc ggcaccggct ctgcggagta tcgggaccgc tgtaagtcga gggccagccg
    27841 ttcgaagtcg acacgcgccg gcccagccag ctcaggggcg aggtcgacgg caaccgcacg
    27901 gctggaaccg tcgggccacg ggatgttgcg atccttggcc agtgcagcga gttcctccca
    27961 ggcactctcg aacggcaacc ctgaatcgcg cagccggcgc gagcgcagcg tccggcgtgc
    28021 caccccgggc ccgaaaccgg ccagggcgac gaccgccagc ccgagcgccg tccagcccag
    28081 ccaggcgggc gggagcccga agccaccggg tgcggtcggc gcggccgtcg cggacgggga
    28141 cgcggacgcc gcgctggggc tggcagtggt cggagtcggc cgggcactcg ggctggccga
    28201 tggcgtgccg ttaccagcaa ccgcgcccgg cggcgtcgcg tccacggcga tccagccgat
    28261 gtcatcgccg aagtagagct ccgcccaggc gtgcatgttc cgcggcgaga cctgccagcg
    28321 ttgtccgtcc ctcgtgccgg gaaggaaacc gatcactacc cgggaaggga tgccggccat
    28381 ccgggaaagc acggccatgg cgccggcgaa ctgctcgcag tagccgatcc gccgcccgac
    28441 caggaagtcg ttcagggtgt ccatcgtcgt cccgggtgcc gtgaccgtgc tgtaggtgta
    28501 ctcgccggag cgaaggtggt cgcggatggc gatcgcgcgt tcgccggctg tggttgtgcc
    28561 ctcgaacagg gtctgggtca actcgactac atcgggggag atcccttcgg gcacgttcag
    28621 gctgatgccg tcgtcgccca ccgcgccggc ttccgtcagc tgtggcagca gttggtcgag
    28681 ggagaacatc cgtgcgctca ccaccgaata cgaggtcttc cgggacgccg tccggcgatc
    28741 gtcaccgccc gccaccaccg cgagggtgcg cgggtcgtac cgccactcac cggcgaccgc
    28801 aacgttgagc ggcgcccacg gcaccggcag gtactcgctg gcgaactcgc cgacctcgat
    28861 ctctgtgcgg gacctgtcgg agtacaggtc ctcggcgatg ttgttgcgca gcggcaggcc
    28921 caccagttcg gtagcggaca ggtggaaccc ggtgttgtcc agcaccggca gcgctgccag
    28981 tcgcagatac tccccggtct cgctggaggt gcggtaggtg atcaccggct tgtcgctggt
    29041 tgagttcagg ttccggatca ggtcgagact cggatcgccc agttgcaccg cgcccgggcc
    29101 gctcgggccc gaccagtcgc ggtaccgctc cgggatggcc agcccagcga tgagcgtgcc
    29161 gacgagcgcg gccaccgaag ctgccagcgc ggcccgtgcg acacctgagc gccaactctc
    29221 cgcagccatg cgctcgggcc cggtcggcac gtggtcaccg ctgtcgggtt cggcccggtt
    29281 gcgggtgtcg gtggccagca gcaacgcata gcccgcggcc ggcagtagga acagccaggg
    29341 cggggcttcg agataggcca ccagcgcggt gatgaggtac ggcgtgaaca gcgcagggaa
    29401 ggtccaggcc ggtgtgccaa gcccagcggc catcgactcg acgaccaggt agagcagcca
    29461 cagcagcagt acgcagaaca ccgcgaagcc gatgtggaac tccatcggcg ccaccgattc
    29521 ctgcacgaag ccgaccgttt cttcgatcag ttcgatcggt tcgcgggtgg ccggcgccag
    29581 ccagagcagc agcccggcag gcagcagctg ggtaatgcgg acggtgagtt cgctctcggt
    29641 gaggcggcgg acggccgcgc cgacgccggc gctggtcagc atcaggacca gcgcgagcat
    29701 caggtaggta cggtcggtcg tcagcggggt gagcgcgagc ccggcggaca gcatcgcggc
    29761 tacggcggcc aggctgattc gatccttgcc ggtcaccggg actcctgccg accgaggtta
    29821 cgccaggcgt ccgccacgcc gacgccggcc tccgcggtgc cgcagcgcca accggcggcg
    29881 accagcgtgt tcagcccgat gacgtcgcgg ccgccggagt tcaccaggag cgcccagcag
    29941 gcgcgtcccc gttggcacgc ctcagtcagc gccacgctgt cggcggcctc gacgcggcca
    30001 aggaccgcga tcagcagctg gccctcctcg ccgcgggtac aggtggccat cgcctcgctc
    30061 aggtgccggt tgcccacggt gtccacctcg gccaggtgct cgagcaggac gttctcgcgg
    30121 tcggcgccgg cccggggcgg gctgaccagg ctaccgtcgg ccatcaccag gctcacgtcg
    30181 aagccgtccg cgagcaggtg gacgccgatg ctcgccgcag ccgaaattgc ccactcgaag
    30241 cgctcgtcgg cagcgtgccc gccgtagcta cgcgcccgat tgtccagcac gatcgtcgcg
    30301 atcgggtcac gggaccgctc ctcgcggcgc accatcagct cacccgtccg cgcagtcgag
    30361 cgccagtgga tgcggcgcat gtcatcgccg tggtggtact cccggatcag cacgtcgtca
    30421 gcgctgccgt ggccggtgcg cagcaacgtg gtgtccggag cgacgcccag cccggaagcg
    30481 gcccccaggt tggccagtac ttcgactcgc ggggtgacca ggagttcgct gtaggcgggc
    30541 acgacctggg cggctcgggc cagcccgagg ccgtcgccgc tggtccgctc gaggggaccg
    30601 atccggtagc ggccgcggcg gctggggctg agttggtagc gcaggcgttg cacccaccgg
    30661 ccggcgcgag tgggcaggcc gtaccaggag gtctggccca acgtcgccgg cacccgatcg
    30721 cggacgcggg taacaccgcc gagcatccgc gtgcgtgggg ccagcatgac ctcaacctcg
    30781 gtctgtccgc cgacgatcac ccggctcggt gccacgctgc ggcgggcgct gagcccggtg
    30841 gcgttgggca gcagcaggag ccaactcaac accggcagca ggatgaggaa gagaccgggc
    30901 caccacaggt cccgctggcc gacgaaaccg gcgccgacac accacagcac gccggcgccg
    30961 agcatcgtcc agcctcgtcc ggtcaaccct gcgccggatc tcatcgtgac ctcagcccgc
    31021 tcacctgcgt ccggtgggta ccgggacggc gcggacgcac gcgtggatga gttccgagct
    31081 gctgttcggc ccgcgctgag cctgtgcgtt cagcaggatg cggtgcgcca gggtcggttc
    31141 ggccaggtcg gagatgtctt cggggatcac gtagttgcgt ccggacatcg cggctcgcgc
    31201 cttcgctgcg cgcatcaact gcagcccggc ccgcggcgag gccccgagcc gggtggccga
    31261 ctgcgagcgg gtctcgttca cgatgccgac caggtatgac ttcaggctgg agtggacgaa
    31321 cacgcggcgc acgacctgca ccgcgcgcat cactgcggcc gcatccgtca ctggcaccaa
    31381 cggttcgagc atatcgcgtt cgccgtggtg gtcgagcatt gccacctcgg cttcggtgct
    31441 tgggtagccc atcgagattc gcaggccgaa gcggtcgcgc tgggcctcgg gcaggggata
    31501 ggtgccctcc atttcgatcg ggttctgggt ggcgatcacc aggaagggac gggggagcag
    31561 atgagtggtg gtgtccaccg agacccgccg ctcctccatt gcctccaaca gcgccgactg
    31621 ggtcttgggg gaggcgcggt tgatctcgtc gccgatcacg atgttggcga agatgccgcc
    31681 gggcttgaac tggaactccc cggccgccca caccgagacc ccggtgatgt cgcttggcag
    31741 caggtcggga gtgaactgaa tgcgtcgcac ggtgcagtcg atcgcccggg ccagcgactt
    31801 ggcgagcatg gtcttgccca cgccgggcac gtcctcgatc agcaggtggc tctcggcgag
    31861 cagggccgtg atcgccaggt cgaccgcctc gggctttccc tcgatcacct ggctgaccgt
    31921 ggcccgcacc gcctgcgcca gcgccagcac ctcaccgatc tcggtgtccg tactcgtcat
    31981 cattcgcgac tcctcccgtc cgcccaaccc tattcgcggc gtcctagcgc tggctcaagg
    32041 gtgccgggtt agggcccaca aatcggcacc gaaagggatg ctgggtggtg gaaagtggag
    32101 tagggtggcg ctaagtggag gagagcgaag ggagtgggcg catgtttctc ggcacctaca
    32161 cccccaagct cgacgacaag ggacggttct ttctgcccgc gaaattccgc gatcagttcg
    32221 gatcgggcct ggtcatcacc cggcagcagg accgctgcct ggccatctgg ccgaccgaag
    32281 ccttcatggc cgaggtcgcc gctgcggcca cgggtccctc caccgtccgt ggggtgcggg
    32341 agtaccagcg aatgatggcc tcgggcgcct cggacgaatc gccggacagc cagggacggg
    32401 tcaccgtccc ggttcccttg cgggcctacg cgggcctggc caaggacatc gtggtgatcg
    32461 gggccttcaa ccgcctcgaa gtgtgggacg cgacgacctg ggcggagtat tcggcggcgc
    32521 aggaggaggc cttcgcggcc ttggatgacg tagcacctgc ggcgggatga ccgtcgcttg
    32581 gctgactcgt gcggtgcggc ttcgatccgc tcgtgggccg gctccctggc gcaccttccc
    32641 cggtgccagg taccggctga gcacgcggat cgaaaccgga tcgcacgatt cgaatgacga
    32701 ggatggcggc gcatggctga agtgaccggt ggggcggcgt ccctgcacgt gccggtcatg
    32761 gccgagcgca tcctcgaact gctcgccccg gcgctcggag cgccgggctc ggtgtacgtg
    32821 gacgggaccc tcggcatggg cggacacgcc caactggtgc tcagtgcctg cccccaggct
    32881 cgcttggtcg gcatcgatcg cgatccggac gcgctcgcgc tggccggtga gcggctggcg
    32941 cccttcggtg accgtgtcga actgcaccac gccgtctacc acgaactccc cgacgtgctg
    33001 gccgacgccg gactgaagcg ggtgcaggcg atctgcctcg acctgggcct ctcctcgctg
    33061 cagattgacc gcgccgagcg cggcttcgcc tactcggtgg acgcgccgct cgacatgcgc
    33121 atggatccgg gcgccgaact ctctgcggcc tcgatcatca acacctggcc ggccaaggaa
    33181 ctggcgcgca ttctgcgcgt tcacggcgag gagcggttcg ccaatcgcat cgccgaccgg
    33241 atcgtcgctg cccgtgccga cgcgccgttc acgacctcgg cccggttggt gcaggtgatc
    33301 gcggacgcga tcccggctgc cgcccgacac accgggggac acccggccaa gcgcaccttc
    33361 caagccctgc ggatcgtggt caacggagag ctggagtcgc tcgccggcgt gctgccggca
    33421 ggtctctcgg cgctggccct aggcggacgg atggccgtgc tggcctacca ctccggtgag
    33481 gatcggctgg tcaagcaggc cttcgccaag gcgtccagcg accgggtgcc gcccggtgtg
    33541 ccggcggtcc cggccggcta cgccgccgag ttcagcccgc tcacccgcgg cgccgaacgc
    33601 ccctccgctg ccgagatcga ggccaatccg agggccgcgt ccgcccggct acgtgccatc
    33661 acccgcaacc aggaggtcga ccgatgaccg ctttgctggc tccgcgcccc ggaaagcagg
    33721 gggcgcgtcc gcagctgcgt ccggtcgccc gtccgcgcgg acggatggcc acgatcccgt
    33781 tcgcgctggt gatcgcactt gtgctggccg cgggcacggt cggcctgctc gtgctcacga
    33841 cggccctgca gaaccaggcg ttcgcggtgc agagccgcca acacgaggcg acggtactgg
    33901 agatccagct ggcctcgctg caggccgaag tggcggacgc ccgctcgatc cagcacctcg
    33961 cggtggcggc gcagcagttg ggcatgcgtc caaacccgta cggagcgcag cttcggctgt
    34021 ccgacggcaa ggtgctcggc gaggcgagcg cggtcgtcgg cggtgaagtg cccaccgccc
    34081 gctacctgac caaggagcag gctgaagccc aggtgatcgc cttggacaag gccgaagccg
    34141 agcggatcgc caaggccaag gccgaggcca agcagcgcgc ggaagccaag aaggaagctg
    34201 aggcgaagaa gaaggctgaa gccaagaaga aggccgaagc cgagaagaag gccgaagcca
    34261 agaagaaggc cgaagccaag aagaaggccg aggccaagaa gaaggccgag gccaagaaga
    34321 aggccgaagc ggcgaagaag gccaaagcca agaagggcaa gcagccgtga gccggccgct
    34381 ggatcggttc ttcgcgtccg acgcgtcgaa gaagaagcgg aaggtgaagc ggctgccgct
    34441 ggcgcccacg gtcggacggc tgcgggtgct gctgatctcg atcgccgtcg tcttctcgct
    34501 ggctgctgga cgcgccgtgc aggtgcaggc catcgacgcc accaacgtgg ccgagcaggc
    34561 cgccaaccag atcacggtgg cgcacgacct gccggcgttc cgcggcgaga tcaccgatcg
    34621 caacggtgaa gtgctggcga tgacggccga cacggtgaag atcatcgccg acgcgaagat
    34681 gatcgcaacc aacggccgga tgaacgccga gatgaccaca aaggaccgcg aggtcgcagc
    34741 tgccgcgccg caacagatcg ccgacctcct ggtgcgcttc ctcggcggca ccgccgacga
    34801 gtacctgccg aagctgaccg cgaccggcaa gggccagtcg taccagctga tcgcaaagaa
    34861 tgtgtccggc gccacctacc gcgagatcgc gctcgcgatg accgctgccg ggctggtcgg
    34921 cctgcgctcg gagagcaacc cgacgcgcgt gtatccgaac ggcgccctgg cttcgaacgt
    34981 gctgggcttc ctctccgagg acggcaccgg cgccagcggc ctggagcagg cgct
//
LOCUS       DAOPRQ010000021        34836 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_959_length_34836_cov_13.104712, whole genome shotgun sequence.
ACCESSION   DAOPRQ010000021 DAOPRQ010000000
VERSION     DAOPRQ010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 34836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34836
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_959_length_34836_cov_13.104712"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(15..953)
                     /locus_tag="PKJ61_04225"
     CDS             complement(15..953)
                     /locus_tag="PKJ61_04225"
                     /inference="COORDINATES: protein motif:HMM:NF039905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HOC13064.1"
                     /translation="MSSSQEPAGVVREPGPWAVVANPTKLDDPDATKARFAEICADHG
                     WPEPRWYETTAHDPGEGQAKQAHADGATLVCPLGGDGTVRAVASGLVDAGVPVGLLPG
                     GTGNLLARNLRLPVGDLDAALTVALTGRDLPIDVGEVRWDDGQPAIFLVMAGMGLDAE
                     MMAGVDDGLKRTVGWMAYLISGAKALFKLGFAVRVRAAEERAMSLHARAVVVGNCGEL
                     AGGLALMPEASLTDGKLDTVLASPNSISAWLAVGVHVLTRHRRGHRSLVSMVSDRVVV
                     DTREPVEGQLDGDAVGPRQRMTCEVRPGALVVRMPA"
     gene            complement(1089..1958)
                     /locus_tag="PKJ61_04230"
     CDS             complement(1089..1958)
                     /locus_tag="PKJ61_04230"
                     /inference="COORDINATES: protein motif:HMM:NF013722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HOC13065.1"
                     /translation="MSPADREVELLPRLPKLGPLFAKALLPSLDRGPARIPNRTAMVA
                     GVTQNRARLADYDRVCGFTLRDEVPPTWLHVLTFPLHVHLLSSADSSVRLAGVVHVSN
                     TMSLHRPVRADEVLDVAVSVRNLRPHRRGALLDLVGEVRVADELVWEGVSTYLAPGAS
                     TSGPPDASERPAFEARTPIAGWRLPAKLGVDYRRVSGDPNPIHTNPLAARAFGFKRPI
                     IHGMWTHARLLAALQPRLPDAYRAEVDFTRPILLPAKVGAWWQPTDTGWEAAVTTTDG
                     SKPYLLAKFSALP"
     gene            complement(1958..3382)
                     /locus_tag="PKJ61_04235"
     CDS             complement(1958..3382)
                     /locus_tag="PKJ61_04235"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: protein motif:HMM:NF006110.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HOC13066.1"
                     /translation="MAGILERVFYSGAGQAVAGRLGLPEPEELRRGRTLPTGSVALAS
                     LAGSGLGAETLSLLGLASVDALRDSEAEPPAYAERIGAIVVDACGLREIVQLEDLRAV
                     LRPAVKGLAASGRVILLADAEVDGLEANAVRQGLDGINRTVGKELRRGATSNLVYVTR
                     EVRSANLASTLSFLLEGRSAFVDGQSWTVGAGETASEIDGPAALRDAAGSSLTGSSSG
                     SGFDQVHPLAGRIVVVTGAARGIGAQIAAICARDGARVVCVDVPAAGEALAQVANRLH
                     GTALQLDITAPDAGEQIAAHVASRFGASARIHGIVHNAGITRDKLLANTDAARWAQVL
                     QVNLAAQLRINPILLDGRPGGLADHGRIVGVASTSGIAGNRGQTNYAASKAGVVGVVR
                     AMAPELAGRGITVNAVAPGFIETEMTAKIPFVQREIFRRTNSLGQAGLPVDVAETIGY
                     FLDPASAGVTGQVIRVCGQNLVGA"
     gene            complement(3382..4650)
                     /locus_tag="PKJ61_04240"
     CDS             complement(3382..4650)
                     /locus_tag="PKJ61_04240"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007077867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HOC13067.1"
                     /translation="MALPRTAVIVGGNRTPFAKAGGPYAAASNSDLLTAALDGLVARF
                     GLAGRRLDEVAGGAVLKHTRDFNLTREAVLGSALHPTTAAVDLQQACATGLETVIYVA
                     NKISLGQADVGVGCGVDSASDAPIVVSEPLRRALLAANRARTLPQRLAALAAIRPGQL
                     APVAPAVVEPRTGLSMGEHQAKTAKAWGISREAQDELSFASHQNLAAAWANGFFDDLV
                     TPYLGVTRDTILRPDTTLEKLAALKPVFGDTMTAGNSTALTDGASAVLVADADWAAAE
                     GLPALAKIVDAQVAAVDFPGGDDLLLAPAHAAARLLDRLGMSLGDFELYELHEAFAST
                     VLATLAAWESAEFCRDQLGLAGPLGSIERDKINVSGSSLATGHPFAATGGRIVPTLAK
                     LLHARGPGARGLISICAAGGQGVVAVLEAV"
     gene            4721..5383
                     /locus_tag="PKJ61_04245"
     CDS             4721..5383
                     /locus_tag="PKJ61_04245"
                     /inference="COORDINATES: protein motif:HMM:NF039596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOC13068.1"
                     /translation="MSTTDGRDTRWAEHRETRRRELVEDTLRAIRKHGAGVGMDEIAT
                     QAGTSKTVIYRHFGDRAGLYAAVVDSVHGFIHRGLATAMQLTDPGDLSALTHDLADAY
                     LALVERDPEIYRFVLAPPSPDAASSVDPYGGLPEIMGDHVTAAIADHLRRRGQDPACA
                     GTWGHGLVGFIRAAADRWMASEPREPREAVLSHIDDFFTPALASGLAAVPQLSAPLPQ
                     EH"
     gene            5383..7326
                     /locus_tag="PKJ61_04250"
     CDS             5383..7326
                     /locus_tag="PKJ61_04250"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_function: GO:0003997 - acyl-CoA oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HOC13069.1"
                     /translation="MSLLMDRTDQAAALGAALTRALDGQFADQRADARANFPVENLLR
                     DPCLTIEQARDWVLSRLKELSAAGFGASGMKTSADGDADPAAALIAFEMLAHGDLSVN
                     IKSGVQFGLFGGAVTNLGTAWHHDTYLADITSMKLLGCYAMTELGHGSDVSGLETTIT
                     YVPESDEFEVHSETPSATKAYIGNAARDGRMAVVYGQLLVRGKNHGVHAILVPIRDED
                     GHDLPGVTTGDQGYKGGLLGVDNGTIRFARVRVPRQLLLDRYGGVDASGVYTSPIENP
                     NRRFFTMLGTLVRGRVCIASGGAIAARRGLSIATRYALSRRQFTAPEHPDGVVLLDYL
                     THQRRLFPAIARAYALGFAQNELAAKLVHIQAGGEHTERDQRELETRAAGLKAMATRF
                     ANDTLQTCREACGGAGFMSANQLVGLRADADIYATFEGDNTVLLQLVAKALLTDYKQL
                     WAGLDRVGVAQATARFIGEGVLERTAANRLIERLVATASRRPEDLTLVDRGWHAYLFE
                     ERERHALESLARRMRTASRRGFDALNALGDHLLFVARAHLERVMLEAFIAGIDACTDP
                     DAKAVLERLCSLYALSSISEDRAWFLEHNRISGGRAKAIGAQINALCGELRPDALALV
                     EGFGIPESWLGAELLSRPEWPAA"
     gene            7460..8206
                     /locus_tag="PKJ61_04255"
     CDS             7460..8206
                     /locus_tag="PKJ61_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13070.1"
                     /translation="MGEKSRRWLVSAGLLAGAAPLIAAAVTSYWQPCAGSMLSGSVFN
                     GYRYGPDFSDACLAAMDEAFMFPMPLPGSGWTLAGASGLSALMLLAAAWLVLLPTTRL
                     PWLARLAAASPALFSIALAGLSLVAAVSPADWMTPLMSGLWVLIEVAALVAMVVIGVG
                     GSTGLVFLRYVIVLGASTAAGWFHQIFEYLVAISLSDASWDSPPGTGYFTVLMIAASA
                     LATAGLWAVAGRTAAQRADASGLRQAGQPT"
     gene            complement(8180..8491)
                     /locus_tag="PKJ61_04260"
     CDS             complement(8180..8491)
                     /locus_tag="PKJ61_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013160605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HOC13071.1"
                     /translation="MSLGAWVLFAAATAYLIKLAGYLLPQSFLDRPDVHELAAALTVG
                     LLASLTVMNTLGKGQAVALDARLLSLVAAAIALKLRAPYIVVVVVGALAAGLGRLAGL
                     P"
     gene            complement(8488..9156)
                     /locus_tag="PKJ61_04265"
     CDS             complement(8488..9156)
                     /locus_tag="PKJ61_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013160606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HOC13072.1"
                     /translation="MLKPTPATRMGLSIAAAVGVYGVSFGALSVAAGLNVWQTCALSA
                     LMFTGGSQFAFIGVIAGGGTGAAAWAAATLLGVRNGVYGMQLKALIRPPAKLIPLMAQ
                     LTIDESTATASGQDEHDERVRGFWTAGVGVFILWNLFTLVGALAGDAIGDPGRWGLDG
                     AAVAAFLGLLWPRLTGRDPVAIAIVAAMTTIIALPLVPPGIPVLLAAATTALWWFWRK
                     RRTA"
     gene            9264..9908
                     /locus_tag="PKJ61_04270"
     CDS             9264..9908
                     /locus_tag="PKJ61_04270"
                     /inference="COORDINATES: protein motif:HMM:NF024304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOC13073.1"
                     /translation="MQRTLTPSRMAGRGRTDRQALDELLDEVLVGYLGVSLDEGPLVL
                     PVGFARDGDRVLLHGSTGSHRMRVLAEGADVCFTVATMDALKVARSAFGTGMQYRSAC
                     LFGVCEVLEGDDKASALAAYTNRYLPGRIDEVRPITAKERAATMVLALPIETWSMKVA
                     GGFPDDDPDDVAGDAWAGVVPLLTGYGAPLRNPDLRVGVGVPASVLALQPKVEV"
     gene            complement(10036..11361)
                     /locus_tag="PKJ61_04275"
     CDS             complement(10036..11361)
                     /locus_tag="PKJ61_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008361145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HOC13074.1"
                     /translation="MALWTLHGDGTLQPGAVVAPDERLGWGKMFGLGAQHVVAMFGAT
                     FLVPLITGFPIPATLFFSGVGTLIFLLITGNKLPSYLGSSFAFISPVMASKVDGDIGK
                     ALFGIMLAGILLAIVGAVVHLAGSAWIDALMPPVMTGTIVMLIGFNLASAAWGVNETS
                     GFRAAPLTGWITIAAIVLITVLFRGLIGRLSILLGVLVGYVAALFQGQVDFKSVGEAA
                     WFGLPAFTLPTPDISVTALFLPVVLVLIAENVGHVKSVALMTGRNYDKSMGRALLADG
                     VATTIAGLGGGSGTTTYAENIGVMAATKVYSTALYWIAGCIAITLSFIPKFGSAIATI
                     PAGVIGAAGTVLYGMIGLLGARIWIENKVVFTNPVNLIPAAVGLIMGIADFNFTIGNM
                     TFGGVTVGTVTALALFHLMKAIAKARGTDLEEPAITTDFEPQHEPDEVR"
     gene            complement(11513..13018)
                     /locus_tag="PKJ61_04280"
     CDS             complement(11513..13018)
                     /locus_tag="PKJ61_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007433393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HOC13075.1"
                     /translation="MGWAVTIVITVIAGLLRFQGLAYPNKLIFDETYYAKDAWTLLHF
                     GYEKAWPKDPNDAVTSGDVNSYLTTAEYVVHPPVGKWLIALGEQWFGMNSFGWRFMPF
                     VFGTLLVFITIRLARRLSRSTLIGGIAGILLTLDGLAFTMSRTALLDIFQAFFLVAGV
                     ACVVADRDWYRRWLAARLDALGVPDFGGRFGPILWLRPWRLLAGLMFGLAIGTKWNSM
                     FVLAAMGVLSVLWDVGARRLAGADWHSWLALLVDGIPAFVRMVLVAVLVYIASWWGWL
                     TTSGGYYRDWGATHADHPWTIHLGEMWASFLHYQQEIFNFHTGEYINGVTHSYASNPW
                     GWLLLARPTGFDAVNGIKPGVDGCWATGDDTCLRVISAIGTPALWWMAALALVVAIIW
                     WLGGRDWRFGVPVVAALSTYVPWLLTTGGRPLFFFYAITIIPFTVIALSLVFGLILGP
                     ADSPKRRRGAIIVGVAMALVAINFAYIYPVLTDQMMLYKDWLARMWLRSWI"
     gene            13352..14209
                     /gene="rsmI"
                     /locus_tag="PKJ61_04285"
     CDS             13352..14209
                     /gene="rsmI"
                     /locus_tag="PKJ61_04285"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016669317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOC13076.1"
                     /translation="MDDRLAGGVLVLAGTPIGNVSDASAALLEALAGADLIAAEDTRR
                     LRDLVSRLEISTRARVVSYFDGNEASRVDELLAALRDGLRVVVVSDAGMPTVSDPGYR
                     VVAAATAAGLPVRVLPGPSAVLVALALSGLPTDRFCFEGFPPRKGGERRRRLAELATE
                     ARTMVFFEAPHRLADFLADARAAFGDDRLAAVSRELTKTYEETRRGSLAELAAWASAG
                     VRGEITVVISGAAAAAVTVADALPLVSERVAGGEPFSAAVAAVAADAGLSRKALYQAA
                     LAARKDADG"
     gene            14202..15140
                     /locus_tag="PKJ61_04290"
     CDS             14202..15140
                     /locus_tag="PKJ61_04290"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016669318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOC13077.1"
                     /translation="MADEFGSPSVDLDKLDQPGAGSTLAELDEALAHLNLPLRPESLP
                     VPTTDAHTHAGSTTEYSKLSLEHILAAARSVNVTRIVEVGTDVVSSRQAIALADTQPG
                     VVAAVAIHPNDAARLGARLPAELAALEALVGSSTRVRGVGETGLDYFRTKDPAGQALQ
                     KEAFAAHIAWAKTHDLALVIHDRDAHADILDVLAAEGAPDRVQMHCFSGDAEFARACL
                     GRGYWLSFPGTVTFKPNEALRQALDLTPPDKLLVETDAPYLTPLPYRGRANSSYLLPH
                     TVRFIAERRGVDLAELCAQLATNASAVFGDWGDGDA"
     gene            15359..16255
                     /gene="rsmA"
                     /locus_tag="PKJ61_04295"
     CDS             15359..16255
                     /gene="rsmA"
                     /locus_tag="PKJ61_04295"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013161613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HOC13078.1"
                     /translation="MADGLLDPRTIRELAAELDLRPTKQRGQNFVHDPNTVRRIVGAA
                     GLGAEDVVLEVGPGLGSLTLGLLEQVSQVIAIEIEPSLAARLPRTAAQRLPARADRLR
                     VIEADALTVDVIPDPQPTAVVANLPYNVSVPVLLHVLARFPGIRSGLVMVQAEVADRL
                     VAPPGSKTYGIPSVKLAWYAAARRVGSVPPKVFWPVPNVDSGLVELIRRDPPTTTASR
                     EQVFAVVDTAFAQRRKMLRSALSGLFGSSAAASAALEGAGVDPQARGEVLGVSDFVRI
                     AEALADNPRGLASVHGIPGDRD"
     gene            16230..17156
                     /locus_tag="PKJ61_04300"
     CDS             16230..17156
                     /locus_tag="PKJ61_04300"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HOC13079.1"
                     /translation="MASPVTETEAVRVRVAGKVNLALRSGPRRPDGYHSLATVFQAVS
                     VFDEVEATWAKPGQITVSVVGEQADLVPSGESNLAVKAARLLAATAGAGQPLGAELLV
                     RKAIPVTGGMAGGSADAAGALLACAVLWDLDVTPDELRELGAQLGADVPFCLAGGTAL
                     GTGRGDQLAPVLSRGTYHWVLAFNEAELSTPAVFAKFDELGLATEPLTVPSELLNALV
                     SGDVQALGRTLVNDLEPAALALRPELRQVLDAGRELGAIAAMVSGSGPTVAFLAPNEP
                     AAVDLSVKLSGAGLCRAVKRVSGPVPGARLVV"
     gene            complement(17246..18427)
                     /locus_tag="PKJ61_04305"
     CDS             complement(17246..18427)
                     /locus_tag="PKJ61_04305"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOC13080.1"
                     /translation="MISLGSPKAALAVVLLFFYNGLVIGVYAAAIPTLVLRFNLAPIH
                     LSMLFVAIGLAAIASMQASGRLADRFGARRISLAMIPVLAIGILGIGLAPTLPVLVAS
                     AILLGLGNGGIDVAMNAVGVQVEQNRPRPIMSFFHGMWSVGNLVGAALIVGLAPFFAR
                     NPEPTVAAAAAIASALGLVALGVAWRITPETAVISHADETGAKTPIPRSAYLLGLMAI
                     AFGLGEGTANDWSGLHVTQIAGVDATTGALGFTCVAAFMVVIRLFGDFLVTRFGRRAV
                     TGFGGACSALGYLIAATGATLPVLLIGWSLVGLGIGMIAPQVYAVAGHTAGGRGLAVV
                     VTFGYATFLIAPALVGMLVQTIGIQHTMFVPAALLLGLLLVARIMPGREVAVEELSDA
                     R"
     gene            complement(18524..19201)
                     /locus_tag="PKJ61_04310"
     CDS             complement(18524..19201)
                     /locus_tag="PKJ61_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002516631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOC13081.1"
                     /translation="MSSAAATSRARRSRVRMTRAERREQLIDVARALFAERGLEGTSV
                     EEIAAHAGVSKPVVYEHFGGKEGLYAVVVDREVRTLHDAIRTALTTPRANPRALIELG
                     ALALLDYIDDHPNGFRIISRDSSATSPEGSFATILSDIASRVEGLLANEFTKRELDAQ
                     TAPMYAQMLVGLVALAGQWWMDTREEQQLDKPVVAAHLVNLAWYGMRNLRPNPTLYVR
                     ERAERDS"
     gene            19463..19541
                     /locus_tag="PKJ61_04315"
     tRNA            19463..19541
                     /locus_tag="PKJ61_04315"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19498..19500,aa:Gln,seq:ttg)
     gene            complement(19634..20014)
                     /locus_tag="PKJ61_04320"
     CDS             complement(19634..20014)
                     /locus_tag="PKJ61_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13082.1"
                     /translation="MTPRTERVIFIAHGVIMLAAAIVLVVFPAAIPATVGIALARSEY
                     LLPYVLAAPELGIGLLSLGAARLRDPDAIRLIALSFAAFHGATAVLEVIPLAAIGVSG
                     VLIANIVVRMLVSGLFVLIARRRR"
     gene            20205..21674
                     /gene="glmU"
                     /locus_tag="PKJ61_04325"
     CDS             20205..21674
                     /gene="glmU"
                     /locus_tag="PKJ61_04325"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016669323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HOC13083.1"
                     /translation="MAEQDAVVDNRVAAVVIMAAGAGTRMRSSVPKVLHPIAGKSMIA
                     LAIDTADALAPERLVVVVGHQREQVTAHLAEVAPHVTIAVQAQLLGTGDAVRAGLAAL
                     PGISGAVVVTSGDVPLLAGETLQGLVATHRSEGNAITVLTSIVPDPTGYGRIVREGDH
                     VARIVEQKDASPDELDLNEINSGIYVFEADVLGEGIEALEAKNAQGELYLTDVIAYAR
                     QAGRRVGAQLLADHLQTEGVNDRIQLAERGAELNRRILNRWMAAGVTVIDPATTWVHA
                     GVDLAEDVTLLPGTSLEGATSVASGAIIGPETTLIDVEVGENARVLRTHAELVVIGPE
                     ATVGPFTYMRPGTELAARVHVGAFVETKKAIIGEGTKVPHLTYCGDARIGAGSNIGAG
                     TIFANYDGVGKYTTTVGSHSFVGSDSVLVAPVEIADGAYVAAGSTITGDVGPGELAVA
                     RGQQRNIAGWVARKRAGTKSASAAAAALAAKAEAPGASE"
     gene            21671..22648
                     /locus_tag="PKJ61_04330"
     CDS             21671..22648
                     /locus_tag="PKJ61_04330"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016669324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0044249 - cellular biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HOC13084.1"
                     /translation="MSGIKRPTEKHLMLCTGRAFPELAEDIAKLLGVDLVPTRSLTYA
                     NSEIYVRFEESVRGADAFVIQSHPAPVNEWLMEQLIMIDALKRASAKRITVVSPFYPY
                     GRQDKKHAGREPISARLVADLYKTAGANRLMSIDLHAAQIQGFFDGPVDHLLALPVLV
                     KYVKENYDVSEMTVVSPDAGRVRLADMWSDQLGTPLAIIHKRRDPNKANEVAVGEVVG
                     DVNGRVCLLVDDMIDTAGTICQAAAALKAHGAKAVIAAATHPILSGPAPQRLNESEFE
                     EVIVTNTLPISEGVNIPKLTVLSMAPLIAQAIQAVFEDGSVASLFNGAH"
     gene            22837..23448
                     /locus_tag="PKJ61_04335"
     CDS             22837..23448
                     /locus_tag="PKJ61_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HOC13085.1"
                     /translation="MTDSKIAAVTRTEFGKGAARRTRRAGLVPAVLYGHGTDPVHLSL
                     PGHEVQLALRVANAVLELSVDGGKTELALAKQAQRNPIKGNVEHLDLVIVKKGEKVTV
                     EVPLLVVGDHPADRMIVMDQQTISLEVEATHIPTGVEINIAGLEIGDTVTAKDLVLPK
                     GAVFPGEPDDLILSVAATPSQAALDAELAGTDEGAEAEAAEEA"
     gene            23458..24060
                     /gene="pth"
                     /locus_tag="PKJ61_04340"
     CDS             23458..24060
                     /gene="pth"
                     /locus_tag="PKJ61_04340"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013161604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOC13086.1"
                     /translation="MADWLVVGLGNPGPTYASHRHNVGFMVVDELARRAGVRFSRSLT
                     LRAETAQTRITASGGIGGIGADADRVVLLKSRTFMNESGIAVSKAANYFKTPADHLVV
                     VHDELDLDFGRIRLKFGGGDNGHNGLKSIRRHLGTGDFFRARYGVGRPPGRQVAADYV
                     LSPFPSALSEDVAVEVSRCADAVESLLTQGLDATQGRFNS"
     gene            24101..27694
                     /gene="mfd"
                     /locus_tag="PKJ61_04345"
     CDS             24101..27694
                     /gene="mfd"
                     /locus_tag="PKJ61_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020391392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HOC13087.1"
                     /translation="MILDGLIELVGSEPVIAEAIADSDTGRLPALDLTMPEPLRPVFI
                     AALARRTSRTILLVTSTFREAEALTSACTSLLDEHDVAYYPAWETLPHERLSPRSDTV
                     GRRLAVLRRLAGNDVLPAPRVVIAPVRSVLQPQVVGLGNLLPVTLVTGEDYGLEDVAR
                     GLVDAAYIRVDLVERRGEFCVRGGIIDIFPPTLEHPVRVDFFGDTVEEIRPFSVADQR
                     SFDETLPEVVASPCRELLITESVKARAAELARTHLNAPGNLGELFDKIASGHAVDGVE
                     ALAPVLVDGLELLIDVLPADTQILLSDPERIRARAHELVITSEEFLRASWAAAAEGGV
                     APIDLGASAYRSLADVRAHALELGQAWWSLSPFSAGPDESADGISPATVESATVSAWD
                     VSAVPSRTVQATEAPVWRGDTEAAVASIRESAAAKHRTILVADSSGLAARLVEILKSA
                     DIPASRPEQLAETPEPGTVTVVRAELRHGFSLPGSDLDVFTAGDLSGQREADKASRKM
                     PARRKNQIDPLELKSGDSVVHETHGVGRYVEMVQRTVAGATREYLVLDYASSKRGQPA
                     DRLFVPMDQLQLVTRYVGGENPTLDRLGGADWKNRKQRARKAVRQIAAELIKLYAARQ
                     ASRGHAFAPDTVWQNELEDAFAYVETPDQLSAITEVKADMEQVVPMDRLICGDVGYGK
                     TEIAVRAAFKAVMDGKQVAVLVPTTLLVQQHHATFADRYAGFPVTVSALSRFQTEAEK
                     KKVVAGLADGGIDVVVGTHRLFSDQIDFKDLGLVIIDEEQRFGVEHKEALKKLRLNVD
                     VLSMSATPIPRTLEMAITGIREMSVIATPPEERHPVLTFAGPYDAGQVRAAIRRELAR
                     EGQAFYIHNRVQSIEKTAQQIAELVPEARVAVAHGQMNEHTLEQVMVDFWERRFDVLV
                     CTTIVEAGLDISTANTLLIERADLLGLSQLHQLRGRVGRGRERGYAYFLYPPEKPLTE
                     TAHDRLATMAAHTDLGAGMAIAMKDLEIRGAGNLLGGEQSGHIAEVGFDLYIRLVGEA
                     VAEFRGEDTEPEPEMKIELPVDAHLPGDYVSSERLRLEMYKRLAAVTSAADIAAVREE
                     LVDRYGEPPAPAEALLAVAAFRLAARAHGVNEVVAQGNMIRFAPAVLPDSAQLRLSRL
                     YPGSQVRKATELILVPRPMTRPIGGQPITDAPLLEWASKVLSDVFTVPASR"
     gene            27703..28257
                     /locus_tag="PKJ61_04350"
     CDS             27703..28257
                     /locus_tag="PKJ61_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13088.1"
                     /translation="MAHETKVGQMHKAAFKLAGLVAGTVALVGCAGGNPTVAAYVGAE
                     TISQAKVDGVARVLADATADATDTAGGYGQTVVAILVQSKLAAQAAATKGITVSDAER
                     QQVYDSNELYTVLLNNPATADFMREYANTAVIFSTEEGKAGVNELLGKTTITVNPRLG
                     TWDAAMGGVVQGSTGSLSELASAK"
     gene            28299..28892
                     /locus_tag="PKJ61_04355"
     CDS             28299..28892
                     /locus_tag="PKJ61_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG family protein"
                     /protein_id="HOC13089.1"
                     /translation="MHRLRSGCPWDAEQTHLSLVRYLVEETLEVVEAIEAGDDDQLTE
                     ELGDLLLQVVFHAEIAAESRRFDIEDVARRIADKLVSRHPYVFAEADVPSDLVSSWER
                     AKAVEKGRDSALDGIPERLSALTRAQKVLGRSRSHGLDLASLGVEPADQAAPELGTAF
                     LSLVARADELGLDAEQAARSALRDLESRVRVAEANSR"
     assembly_gap    29042..29141
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            29380..30663
                     /gene="eno"
                     /locus_tag="PKJ61_04360"
     CDS             29380..30663
                     /gene="eno"
                     /locus_tag="PKJ61_04360"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002516702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOC13090.1"
                     /translation="MALIEFVHARQILDSRGNPTVEVQVVLDDDTEGRAAVPSGASTG
                     AFEAAELRDEDAAHYGGKGVLKAVENVVEQIAPEVIGMEASEQRLLDQAMIELDGTDN
                     KSKLGANAILGVSLAVAQAAAESAGLPLYRYLGGPTASILPVPMMNILNGGSHADSNV
                     DIQEFMIAPIGAATFADALEMGAGVYHALKAVLKGRGLSTGLGDEGGFAPNLESNAAA
                     LDLILEAIGNAGFVPGKDVALALDVAASEFYNEDGTYNFEGGKKTAAEMTAYYAELVA
                     KYPLVSIEDPLNEDDWDGWKTITDELGDKVQLVGDDLFVTNVSRLQKGIDTQTANALL
                     VKVNQIGSLTETIDAVDMAHRAGYKTMMSHRSGETEDVTIADLAVALGCGQIKSGAPA
                     RSERVAKYNQLLRIEEDLDDAARYAGASAFPRFKA"
     gene            30838..31401
                     /locus_tag="PKJ61_04365"
     CDS             30838..31401
                     /locus_tag="PKJ61_04365"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HOC13091.1"
                     /translation="MARGTRSPRNDTAGPGRPTRRTRAGSRASGPDTPSEPPPRDRRD
                     EVPVWRRGLHVTRRAIVLILVVVALVISFGGSLRGYLAQQHELAVAEQEIRDRSAQIA
                     DLEAELARWDDPDYVKAQARDRLGWVMPGETGYRVIGADGKPIGGGVVIESAEKLPAG
                     EHEPVWWDRLWGSMQAADAPARKVTSP"
     gene            31589..32146
                     /locus_tag="PKJ61_04370"
     CDS             31589..32146
                     /locus_tag="PKJ61_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002516630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF501 domain-containing protein"
                     /protein_id="HOC13092.1"
                     /translation="MPSFEPLTQSDVAAVTAQLGREPRGSAGVAWRCPCGKPGVVATN
                     PRLPDGTPFPTTYYLTCPRATAGCSTLEAEGLMADMTERLTSDPELASGYTAAHEAYL
                     ADRAELGDVTEIAGISAGGMPSRVKCLHVLAAHSLAAGPGVNPLGDETLTRLGEFWSR
                     PCLSATDPEISPDQPGREPEPADRG"
     gene            32139..33065
                     /locus_tag="PKJ61_04375"
     CDS             32139..33065
                     /locus_tag="PKJ61_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002526958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HOC13093.1"
                     /translation="MAELFAAIDCGTNSVRLLITRLVDGRLEEVDRRLHITRLGQGVD
                     ATGAFHPDALARTLDAMADFGAQLDGSGVQHRRVVATSAARDAANSAEFFEGVKARVG
                     VPAEIISGAEEAYLSFTGAVTALPHLPQPVLVMDIGGGSTELVVGSPHGTDVAISLNI
                     GSVRLRESHLHSDPPSAAEVATASVRIDELLDDSGIDFRMAASWVGVGGTVTSLSALA
                     QGLHTYDRTRVHGSVLSQPALSALTARLLAMPIAEVAALPTMVPGRADVICAGALICA
                     RVGARMSRTDLTVSEADILDGIIARLATEAGM"
     gene            33166..33531
                     /locus_tag="PKJ61_04380"
     CDS             33166..33531
                     /locus_tag="PKJ61_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13094.1"
                     /translation="MSPNSLRTAKGGRPVRRVSLPSRDAQGGFTREEDPWSLSGHALT
                     AVGTEMDAIAAVVIGGTLLTGGYGFVLGSALGVLVLGLIQTIISFEGTLSSWWTKIFI
                     GGLLLVFIILQKVLTSRRT"
     gene            33716..34600
                     /locus_tag="PKJ61_04385"
     CDS             33716..34600
                     /locus_tag="PKJ61_04385"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOC13095.1"
                     /translation="MERAIKQLRYIPSPAARALVTRRSRTIGLITTDSPDFGPSTSAV
                     HINEAARDERYAVIMASLRRTDAPALRQAAEVLVRQNVEAIILITSSRSVLGILQGME
                     LGVPFLAVASQGSGLHRVSLDQYQGARRAVRHLIELGHTDIRHVSGPSDSMDAIERIR
                     GWRDALGEHGLVAHEPLPGDWSPDSGYPAGRHLLNETLTAVFCANDQMALGLMHALTE
                     AGRSIPGQVSVVGFDVIPEAAHFSPPADHHAPGLCASGQGRDGADASCAQRLRHPNPL
                     LRLPQLIERGSAAPPQRR"
     gene            34761..34834
                     /locus_tag="PKJ61_04390"
     tRNA            34761..34834
                     /locus_tag="PKJ61_04390"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:34795..34797,aa:Leu,seq:taa)
BASE COUNT         5648 a        11819 c        11945 g         5324 t
ORIGIN      
        1 ctatctgcgc ggagtcaagc gggcatccgc actacgagag cccccgggcg cacctcgcag
       61 gtcattcgtt gcctgggtcc gacagcatcg ccgtcgagct ggccctccac cggttcgcgg
      121 gtgtccacga ccacccggtc ggacaccatc gacaccagcg atcgatggcc gcggcggtgc
      181 cgggtcaaca catgtacccc gacggcgagc cacgccgaga tcgagttcgg cgaggccagg
      241 accgtgtcga gcttgccgtc ggtcagcgac gcctcgggca tcagcgccag cccgccggcc
      301 agttcgccgc aattgccgac caccaccgcg cgcgcgtgca gactcatcgc ccgttcctcc
      361 gccgcgcgga cgcggaccgc gaaccccagc ttgaacagcg ccttcgcccc tgaaatcagg
      421 taggccatcc agccgacggt gcgcttcagt ccgtcgtcca ctccggccat catctcggcg
      481 tccagcccca tcccagccat taccaggaag atggcgggct gaccgtcgtc ccagcgcact
      541 tcgccgacgt cgatcggcag gtcccgcccg gtcagggcga cggtaagcgc ggcgtccagg
      601 tcgccgaccg gcagccgaag gttgcgtgcc agcaggttcc cggtaccgcc tggcagcaat
      661 ccgaccggca cgcccgcgtc caccaggccg ctcgccaccg cgcggacggt gccgtcgccg
      721 cccagcgggc agaccagcgt cgccccgtcc gcgtgcgcct gcttcgcctg gccctcgccg
      781 ggatcgtggg cagtggtctc gtaccagcgc ggttcgggcc agccgtggtc ggcacagatc
      841 tcggcgaagc gagccttggt cgcgtccggg tcgtcgagct tggtcgggtt ggcgaccaca
      901 gcccacgggc cgggctcgcg gacgacgccc gctggctcct gactgctgct cactgggcct
      961 ccttggctgg gactttaccg gctgggtctc gaccaggcgg tgcgcgtaca aaacgccctg
     1021 tcgatgtcga gatcggcccg ttccagcact gtcacgcgat cgacagggca aattgtccgg
     1081 acgcctcgtt acggaagcgc cgagaacttc gccaacaggt aaggcttgct gccgtcagtg
     1141 gtggttacgg cggcttccca gccggtgtcg gttggttgcc accaggcgcc gacctttgcc
     1201 ggcagcagga tgggcctggt gaagtcgacc tctgcccgat acgcatcggg cagccgcggt
     1261 tggagcgcag ccagcagtcg cgcgtgcgtc cacatgccgt ggatgatcgg acgcttgaac
     1321 ccgaacgccc tagccgcgag cggattggtg tggatcgggt tcggatcgcc ggacacccgc
     1381 cggtagtcga ccccgagttt ggccggcaac cgccagccgg cgatcggggt gcgggcctcg
     1441 aacgcaggtc gctcggacgc gtccggtggg ccggacgtgc tcgcacccgg cgcgagatag
     1501 gtgctgaccc cttcccagac cagctcgtcg gcgaccctaa cctcgccgac cagatcgagc
     1561 agtgcgcccc ggcggtgcgg ccggaggttc ctgacgctca ctgctacgtc cagcacctcg
     1621 tcggcgcgaa ccgggcggtg cagcgacatg gtgttcgaga cgtgcaccac cccggccaac
     1681 cgcacactcg aatcagcgct tgagagcaga tggacgtgca gcgggaacgt gagcacgtgc
     1741 aaccaggtcg gcggcacctc atcgcgcagg gtgaagccgc agacccggtc gtagtcggcc
     1801 aaacgtgccc ggttctgggt gacgccggcg accatagcgg tgcggttcgg gatgcgcgcc
     1861 ggaccacggt cgaggctcgg cagcagcgcc ttggcgaaca gcggtccgag cttcggcagc
     1921 cggggcagca gctcgacctc gcggtctgcg ggcgacatca ggcgccgacc aggttctggc
     1981 cgcacacccg aatcacctgg ccggttacac cagcggatgc ggggtcgagg aagtagccga
     2041 tggtttcggc cacgtcgacc ggcaagccgg cctggccgag gctgttggtg cgccggaaga
     2101 tctcgcgctg cacgaacggg atcttcgcgg tcatctcggt ctcgatgaag cccggggcga
     2161 cggcgttcac cgtgatgccg cggccggcga gttcgggtgc catcgcccgg acgacgccga
     2221 ccaccccggc cttggatgcc gcgtagttcg tctggccgcg gttcccggcg atgccggagg
     2281 tggacgcgac accgacgatg cgtccgtggt cggccaggcc gccgggacgc ccgtccagca
     2341 ggatcgggtt gatccgcagc tgggccgcca ggttcacctg cagcacctgc gcccagcggg
     2401 cggcgtcggt gttggcgagc agcttgtcgc gggtgatgcc ggcgttgtgc acgatgccgt
     2461 ggatgcgtgc gctcgcaccg aaccgggatg cgacgtgcgc cgcgatctgt tcgcccgcgt
     2521 ccggtgcggt gatgtcgagc tgaagcgcag tgccgtgcag ccggttggcc acctgcgcga
     2581 gcgcctcgcc ggccgccggg acgtcgacgc agaccactct cgcgccgtcc cgcgcgcaga
     2641 ttgctgcgat ctgcgcaccg atgccgcggg ccgcgcccgt gaccacgacg atccggccgg
     2701 ccagcgggtg gacctgatca aaaccgctcc ctgaggagct gccggtgagg gacgagccgg
     2761 cggcgtcacg aagggcggcc gggccatcga tctcagaggc cgtttcgcca gcgcctaccg
     2821 tccaggactg cccgtcgacg aacgccgagc ggccttcgag gaggaacgac agcgtggagg
     2881 caaggttcgc cgatcggacc tcgcgggtca cgtagaccag gttcgaagtg gcgccgcggc
     2941 gcagttcctt gccgacggtg cggttgatgc catccagccc ctggcggacg gcgttcgcct
     3001 ccagcccgtc cacttcggcg tcggccagca ggatcacgcg cccggacgcg gccaagccct
     3061 tcacggccgg acgcagcact gcccgcaagt cctcgagttg aacgatctcg cgcagtccgc
     3121 acgcgtccac gacgatcgcg ccgatgcgct cggcgtaggc gggtggttcg gcttccgagt
     3181 cgcgcagggc gtctaccgaa gccagcccga gcaagctgag cgtctccgcg ccgaggcccg
     3241 agccggcgag actggccaat gcgaccgacc cggtcggcaa cgtgcggccg cggcgaagct
     3301 cttcaggctc gggcaagccg aggcgtccgg caacagcctg gccggcgccg gagtagaaga
     3361 cgcgttcgag aatcccggcc atcacaccgc ctccaggact gcgaccacgc cttggccgcc
     3421 ggcggcgcag atcgagatca gcccgcgcgc gcccgggccg cgcgcgtgca gcagcttcgc
     3481 cagagttggg acaattctgc cgccagtcgc cgcgaaaggg tggccggtgg caagcgagga
     3541 gccactgacg ttgattttgt cccgttctat cgagcccagc ggcccagcca agccgagttg
     3601 gtcgcggcag aactccgccg attcccaggc ggccagggtg gccagcacgg ttgaagcgaa
     3661 cgcctcgtgc agttcgtaca gctcgaagtc gcccagcgac atgccgagac ggtcaagcaa
     3721 gcgggctgcc gcgtgggccg gtgccaacag caggtcgtcg ccgccaggga agtcgaccgc
     3781 ggccacctgt gcgtccacaa ttttcgccaa ggcgggcagc ccctcggcgg ccgcccaatc
     3841 ggcatcggcc accagcacgg cggacgcccc gtcggtcagc gcggtcgagt tgcccgcggt
     3901 catggtgtcg ccgaagaccg gcttcagcgc ggccagcttc tccaaggtgg tgtccggacg
     3961 caggatcgtg tctctggtca cccccagata gggcgtcacc agatcgtcga agaacccatt
     4021 tgcccaagcc gcagccaagt tctgatgcga cgcgaaggag agctcgtcct gtgcctcccg
     4081 ggagataccc cacgccttgg ccgtcttggc ctggtgctca cccatcgaca gaccggttct
     4141 tggctcgacg accgccgggg cgaccggagc caactgcccg ggacgaatcg cggccagcgc
     4201 ggccagtcgc tgcggcaggg tcctggcccg attcgccgcc agcaacgctc ggcgcagcgg
     4261 ctcgctgacc acgatcgggg cgtcggacgc cgaatccacg ccgcagccga cgccgacgtc
     4321 cgcctgaccg aggctgatct tgttcgccac gtagatcacc gtctccagcc cggtggcgca
     4381 ggcctgctgc aggtcgaccg ccgcggtggt ggggtgtagc gccgaaccga gcacggcttc
     4441 gcgggtgagg ttgaagtcgc gggtgtgctt cagcactgcg ccgccggcga cctcgtccag
     4501 ccgacgcccg gcgagcccga aacgggcgac caacccgtcc agcgcggccg tgagtaggtc
     4561 gctgttactg gccgcggcgt acgggccgcc ggccttcgcg aacggcgtcc ggttgccgcc
     4621 gacgatcact gcggtacggg ggagagccat ggaacctcct cggcagattt actcgatacc
     4681 gagggtagca ggtacggcca gtactgggta ccctgggtgt atgagtacca cggacggccg
     4741 cgacacccgc tgggcggagc accgcgaaac ccggcggcgc gagctcgtcg aggacaccct
     4801 gcgcgcgatc cgcaagcacg gcgccggcgt cggcatggac gagatcgcca cccaggccgg
     4861 cacgtcgaag actgtgatct accggcactt cggcgaccgg gccggactgt atgccgccgt
     4921 ggtcgactcg gtgcacgggt tcattcatcg tgggctggcc acggcgatgc agctcaccga
     4981 cccgggagac ctgtccgcgc tcacccacga tctggcggac gcctacctcg ccctggtcga
     5041 acgcgacccc gagatctacc ggttcgtgct cgccccgccg tcgcccgacg ccgcgtccag
     5101 cgtcgacccg tacggcggtc tgcccgagat catgggcgac cacgtgacgg ccgcgatcgc
     5161 cgaccatctt cgccgccgcg gccaggatcc cgcgtgtgcc ggcacctggg gccacggact
     5221 ggtcgggttc atccgggccg cggccgaccg ctggatggcg tccgagccgc gcgagccgcg
     5281 ggaagccgtc ctctcccaca tcgacgattt cttcaccccg gcgctggcct ccgggctggc
     5341 tgccgtcccg cagttgtccg caccacttcc acaggagcac tgatgagctt gttgatggac
     5401 cgtaccgacc aggccgcagc cctcggcgca gccctcaccc gggcactcga tggccagttc
     5461 gccgaccagc gcgccgatgc ccgggcgaac ttcccggtag agaacctgct gcgcgacccg
     5521 tgcttgacca tcgaacaggc ccgcgactgg gtgttgagcc ggctcaagga gttgtcggcc
     5581 gcgggtttcg gcgcgtccgg aatgaagacc agcgcggacg gtgacgccga ccccgcggcg
     5641 gcgctgatcg cgttcgagat gctcgctcac ggcgacctct cggtgaacat caagtccggc
     5701 gtccagttcg ggctgttcgg cggcgcggtg accaacctgg gcacggcctg gcaccacgac
     5761 acctacctcg ccgacatcac ctcgatgaaa ctgctcggct gctacgcgat gaccgagctg
     5821 ggccacggct cggacgtctc cggcctggag acgacgatca cctacgtgcc ggagtcggac
     5881 gagttcgaag tgcattccga gactccgtcc gctaccaagg cctatatcgg caacgctgcc
     5941 cgcgacggcc ggatggcggt ggtctacggg cagttgctgg tgcgcggcaa gaaccatggg
     6001 gtgcacgcga tcctggtgcc gatccgggac gaggacggcc atgatctgcc cggcgtcacc
     6061 accggcgacc agggctacaa gggcgggctg ctgggtgtcg acaacgggac gatccggttc
     6121 gcacgggtcc gggtgccgcg gcagctgctg ctcgatcgct acggcggcgt ggatgcgtcc
     6181 ggcgtctaca cctcgccgat cgagaacccc aaccggcggt tcttcaccat gctcggcacg
     6241 ctcgtgcgcg gccgggtctg catcgcgtcc ggaggtgcga tcgcggcccg acgagggctc
     6301 tcgatcgcga cgcgttacgc cttgtcaaga cggcaattca cggccccaga gcatccggac
     6361 ggggtggtgc tgctcgacta tctgacccac cagcggcggc tcttcccagc gatcgcgcgg
     6421 gcctacgcgc ttggcttcgc acagaacgag ctggccgcca aattggtgca catccaagcg
     6481 ggcggcgaac ataccgagcg ggaccagcgc gagctcgaaa cccgcgccgc cggactgaag
     6541 gcgatggcga cccggttcgc caacgacacg ctgcagacct gccgcgaagc gtgcggagga
     6601 gcaggcttca tgtcggccaa ccagctggtc gggttgcgcg cggacgccga catctacgcc
     6661 accttcgagg gcgacaacac ggtgctgctg cagctggtgg cgaaggcgct gctgaccgac
     6721 tacaagcagc tttgggcggg cctggatcgg gttggggtgg cccaggccac ggcccggttc
     6781 atcggcgagg gcgtgctgga gcgcactgct gcgaatcggc tgatcgagcg gctggtggcg
     6841 accgcgagcc ggcgtccgga ggacctcacg ctggtcgacc gcgggtggca cgcctacctg
     6901 ttcgaggagc gcgaacggca tgcgctggag tcgctggccc gaaggatgcg gacggcctcc
     6961 cggcgtggtt tcgacgcgtt gaacgcgctc ggcgaccacc tgctgttcgt cgccagggcg
     7021 cacctggagc gggtgatgct ggaggcgttc atcgccggaa tcgacgcctg caccgatccg
     7081 gacgcgaagg ctgtgctgga gcggctctgc agcctctacg cgctgagctc gatctcggag
     7141 gatcgggcct ggttcctgga gcacaaccgg atctccggtg gacgcgccaa ggcgatcggc
     7201 gcccagatca acgcgctgtg cggtgagctc cgtccggacg cgctcgcgct ggtcgagggc
     7261 ttcggtatcc ccgagtcctg gctcggcgct gaactgctga gccgcccgga gtggccagca
     7321 gcctgatccg gctcaacctg gatccggagc ttagctgtgg ctgggtgcac cgtccgtggt
     7381 gtgcccagcc acagtctccg tcgggcagac gctggacgat aaccgccgcg cccgatcgcg
     7441 gaggggctat cgtccgagca tgggggagaa gtcgcgacgc tggctggttt cggccggact
     7501 gctggcgggg gcagctccac tgatcgctgc ggcggtgacc agctactggc aaccgtgcgc
     7561 cggcagcatg ctttcgggaa gtgttttcaa cggctaccgc tacggtccgg acttctccga
     7621 cgcctgtctt gccgctatgg acgaagcatt catgttcccg atgccgctgc ctggttcggg
     7681 ttggacactg gccggcgcgt ccggactgtc ggcgctcatg ctgttggccg cggcctggtt
     7741 ggtgctgctg cccacgaccc ggctgccttg gctcgctcgg ctggcggcgg cgtcgccagc
     7801 cctgttctcg atcgcgctgg ccgggctgtc cctggtcgcg gctgtgtcgc cggccgactg
     7861 gatgacgccg ctcatgagcg gcctgtgggt gctgatcgaa gtggcagctc tggtggccat
     7921 ggtggtcatc ggagttgggg gcagcaccgg tctggtcttc ctgcgctacg tgatcgtcct
     7981 tggcgcaagt acggctgcgg gctggttcca tcagatcttc gagtatctgg tggcgatcag
     8041 tctgagtgac gcgagctggg acagtccgcc cggcaccggc tacttcaccg tcctgatgat
     8101 cgctgcgtcc gcgttggcca ccgcaggttt gtgggccgtc gccgggcgga cggctgcgca
     8161 gcgcgcggat gcgtccgggt tacggcaggc cggccagccg acctaacccc gcggcgaggg
     8221 caccaaccac gaccaccacg atgtagggcg ccctcagctt cagtgcgatc gcggccgcca
     8281 cgagcgaaag caggcgggcg tccagggcca cggcctgacc cttgccgagc gtgttcatca
     8341 ccgtgagtga ggcgagcagc ccgaccgtca gcgccgcggc gagttcgtga acgtccggac
     8401 ggtcgaggaa cgactgcggc aacaggtagc cggccagctt gatcaggtag gcggtagccg
     8461 cggcgaacag cacccaggcg ccgagactca tgctgtcctc cgtttccgcc agaaccacca
     8521 cagggcggtc gtcgccgcag ccagcagcac cggtatcccc ggtggcacca ggggcagcgc
     8581 gatgatcgtg gtcatggcgg ccacgatggc gatcgcaacc gggtcgcgtc cggtgagccg
     8641 cggccagagg aggccgagga aggcagcgac cgcggctccg tccaatcccc aacggcccgg
     8701 gtcgccgatc gcatcgccgg ccagggcgcc gaccagggtg aacaggttcc acagaatgaa
     8761 gacaccgacg ccggctgtcc agaagccgcg gacgcgttcg tcatgctcgt cctgacccga
     8821 ggccgtcgcg gtggactcat cgatggtcaa ctgcgccatc agcgggatca gcttggccgg
     8881 gggtcggatg agagccttga gctgcatgcc gtagacgccg ttgcgcacgc ccagcagggt
     8941 ggccgcagcc catgctgcgg cgccggtacc gccgccggcg atcacaccga tgaacgcgaa
     9001 ttgggagccg ccggtgaaca tcagggcgct cagcgcgcag gtctgccaga cgttgaggcc
     9061 ggccgccacc gaaagggcgc cgaaggagac gccgtacacg cccaccgcgg cagcgatcga
     9121 cagccccatg cgcgtggcag gggtgggctt gagcatgcgg gccaagctag cgcatgtcgt
     9181 gatttcgcag ggcgatcgtt tccagccctg gaatcggggc cccgaccagc atccagcttc
     9241 gttatcgcga catagttgtg cccatgcaac gtacgctgac gccctcccgt atggccggac
     9301 gcggccgcac cgaccgccaa gcgctcgacg aactgctcga cgaggtcctg gtcggctacc
     9361 tcggggtcag cctggacgag ggtccgctgg tgctgccggt ggggttcgcc cgcgatggtg
     9421 accgggtgct gctgcacggc tcgaccggtt cgcaccgcat gcgtgtgctg gccgagggcg
     9481 ccgacgtgtg cttcacggtg gccacgatgg acgcgctgaa ggtggcgcgg tcggcgttcg
     9541 gcactggcat gcagtaccgc agcgcgtgcc tgttcggcgt ctgtgaggtg ctggaaggcg
     9601 acgacaaggc gtccgcgctg gctgcgtaca cgaaccgcta cctgcctgga cggatcgacg
     9661 aggtgcgtcc gatcaccgcg aaggagcggg ccgcaaccat ggtgctcgct ctgccgatcg
     9721 agacctggtc gatgaaggtg gccggcggct ttccggacga cgaccccgac gatgtggccg
     9781 gcgacgcctg ggccggtgtg gtgccgctgc tcaccggcta tggggcgccg ctacgcaacc
     9841 ccgacctgcg cgtcggcgtc ggcgtcccgg cgtcagtgct cgccctccaa cccaaggtgg
     9901 aggtctagtc tcccgaaccc gttcggagcg ccgagagcca gccttggcgc cgagaaccag
     9961 cgcacagagc tagctctcgg cggctaaggc tggcgctcgg tattgggacc cgtccgaatc
    10021 agcgagcgtc ctcggtcagc gaacctcgtc cggctcgtgc tgcggctcga agtcggtggt
    10081 gatcgccggc tcctcgagat cagtgccgcg agccttcgcg atggccttca tcaggtggaa
    10141 gagcgccagc gcggtcacgg tgccgacggt cacgcccccg aaggtcatgt tgccgatggt
    10201 gaagttgaag tcggcgatgc ccatgatcag gccgacggcg gccgggatca ggttcaccgg
    10261 gttggtgaag acgaccttgt tctcgatcca gatccgggcg ccgagcaggc cgatcatgcc
    10321 gtagagcacg gtgccggccg cgccgatcac gccggccgga atcgtggcga tggccgagcc
    10381 gaacttcggg atgaacgaga gcgtgatggc gatgcagccg gcgatccagt acagcgccgt
    10441 cgaatagacc ttggtggctg ccatcacgcc gatgttctcg gcgtaggtag tggtgccgga
    10501 gccgccgccg agaccggcga tggtggtggc cacgccgtcg gcgagcagcg cgcgtcccat
    10561 ggacttgtcg tagttgcgtc cggtcatcag tgcgaccgac ttcacgtgtc cgacgttctc
    10621 tgcgatcagc accagcacca ccgggaggaa gagcgcggtg acgctgatgt cgggggtcgg
    10681 cagggtgaag gcgggaaggc cgaaccaggc tgcttcgccg accgacttga agtccacctg
    10741 gccctggaat agtgcggcga cgtagcccac cagcacgccc agcaggatcg agagccgtcc
    10801 gatcaagcca cggaacaaga cggtgatcag cacgatcgcg gcgatcgtga tccagccggt
    10861 gagcggcgcg gcgcggaaac cggacgtctc gttgacaccc cacgctgcgc tggccaggtt
    10921 gaagccgatc agcatgacga tcgtgccggt catcaccggc ggcatcagcg cgtcgatcca
    10981 cgccgagccg gccaggtgca cgaccgcacc gacgatcgcg agcaggatgc cggccagcat
    11041 gatgccgaac agcgccttgc cgatgtcgcc gtcgaccttc gaggccatca ccggcgagat
    11101 gaaggcgaag gacgagccga gatagctcgg cagcttgttg ccggtgatca gcaggaagat
    11161 cagcgtgccg acgccgctga agaagagcgt tgcggggatc gggaagccgg tgatcagcgg
    11221 caccaggaag gtggcgccga acatggccac cacatgctgg gcgccgagcc cgaacatctt
    11281 gccccaaccg aggcgttcat caggggcgac gactgctccc ggttgcagag tgccgtcgcc
    11341 gtgcagcgtc cataaggcca tggtggagtt cccctttgtc gagcagccgt gcgaactcta
    11401 ttgccactcg gacgccggct cgtcggccgc gaaaaagtga gatgcccttc ggcagtctca
    11461 gcgaacgttc gtcaggctca gacccaacgc ccggctcagg gagcgttggg gcctagatcc
    11521 aggagcgcag ccacatgcgg gcgagccaat ccttgtagag catcatctga tcggtcagca
    11581 cggggtagat gtaggcgaag ttgatcgcca ccagcgccat cgccacaccg acgatgatcg
    11641 ctccgcgccg tcgtttcggg ctgtcggccg gcccaaggat cagcccgaac accagggaca
    11701 gcgcgatcac cgtgaacggg atgatcgtga tcgcataaaa gaagaacagc ggacgcccgc
    11761 ccgtggtcag aagccacggt acgtaagtcg agagcgccgc gacgaccggg acgccgaagc
    11821 gccagtcgcg tccgccgagc caccagatga tcgccaccac cagcgccaac gcggccatcc
    11881 accacaacgc cggggtgccg atcgccgaga tcacccgcag gcaggtgtcg tcgccggtgg
    11941 cccagcaccc gtccacgccc ggcttgatgc cgttgaccgc gtcgaagccg gtgggccgcg
    12001 ccagcagcag ccagccccac gggttcgagg cgtaagaatg ggtcaccccg ttgatgtact
    12061 cgccggtgtg gaagttgaag atctcctgct ggtagtgcag gaacgaggcc cacatctcgc
    12121 cgaggtggat cgtccacgga tggtcggcgt gggttgcgcc ccaatcccgg tagtaccccc
    12181 cggacgtggt cagccagccc caccaactcg cgatgtacac gagcacggcc accagcacca
    12241 tccggacgaa cgcggggatg ccgtccacca acagggccag ccagctgtgc cagtcagccc
    12301 cggccaaccg gcgcgcgccc acgtcccaca gcacgctgag cacgcccatc gcggccagca
    12361 cgaacattga gttccacttc gtgccgatcg cgaggccgaa catcaggccg gccagcaacc
    12421 gccagggacg cagccagagg atcgggccga aacgtccgcc gaagtcaggg acgccgagcg
    12481 cgtccagccg ggcggccaac cagcggcggt accagtcgcg gtcggcgacc acgcaggcaa
    12541 cgccggctac caggaagaac gcttggaaga tgtccagcag ggcggtgcgg gacatggtga
    12601 acgcgaggcc gtcgagggtc agcaggatgc ccgcgatgcc gccgatgagc gtggagcggg
    12661 acaaccgccg agccagccga atggtgatga acaccagcag cgtgccgaac acgaacggca
    12721 tgaatcgcca gccgaacgaa ttcatgccga accactgctc gccgagcgcg atcagccact
    12781 tgcctaccgg cgggtgcacc acgtactcgg cggtggtcag gtagctgttg acgtcgccgg
    12841 acgtgaccgc gtcgttggga tccttcggcc aggccttctc gtagccgaag tgcagcagcg
    12901 tccaggcgtc cttggcgtag taggtctcgt cgaagatcag cttgttgggg taggcgaggc
    12961 cctggaagcg cagcagtccg gcgatgaccg tgatcacgat ggtgaccgcc cagcccagcg
    13021 cccgatcgcg aagctgggcc tgcccgtcgc ggagccgctc gaccgtggtg aggccctcgc
    13081 ggttcaccgc ccggcgcaac cgcggccaag cgcgcttggg ttctccggaa tcgtcgggct
    13141 ggtcgaggcc ggcaagttct gcggcccagg cttgctcatc ggcgtccgcg gcttcctcgg
    13201 tgacgtccgc taccaaccga gtttcagggt ggctcggctc gccacgggac gcgttcgcgt
    13261 ccggccccca ttcggcttcg gtcactcgca catcctaggt gggcgtccgg gtaggcatgc
    13321 cgcacgcgcg cggatcgggg atactgcacg ggtggacgac aggttggctg ggggtgtgct
    13381 ggtgctggcc ggcaccccga tcggcaatgt ctcggacgcc tccgcggcct tgctagaagc
    13441 gctcgccggt gccgatctga tcgctgcgga ggacacccga cggctgcgcg acttggtttc
    13501 ccggctggag atctcgacca gagctcgggt ggtttcctat ttcgacggca acgaggcttc
    13561 tcgggtggac gagctgctgg ccgcgctgcg ggacggactg cgcgtcgtgg tggtgagcga
    13621 cgcggggatg ccgacggtgt ccgatccggg ctatcgggtg gtggccgcgg cgactgcggc
    13681 ggggttgccg gtgcgggtgt tgcccggacc gtccgcggtg ctggtggcgc tggcgttgag
    13741 cggcctgccg accgaccggt tctgcttcga ggggttcccg ccgcgaaagg gtggcgagcg
    13801 gcgacgacgg ttggccgagc tggccaccga ggcgcgcacc atggtgttct tcgaggcccc
    13861 gcatcggctt gccgatttcc tggcggacgc gcgtgctgcg ttcggggacg accggctggc
    13921 ggcggttagc cgggagctga ccaagaccta tgaggagacc cggcgggggt ctttggccga
    13981 gctcgcggct tgggcgtccg ctggcgtgcg cggtgagatc acggtggtga tctcgggggc
    14041 cgccgctgcg gcggtcacgg tggcggacgc gttgccgctg gtttccgaaa gagtggccgg
    14101 tggggaaccg ttctcagctg cggtcgccgc tgtggctgcg gacgccggac tgtcccgcaa
    14161 agcgctctac caggcggcgc tcgccgcgcg aaaggatgcc gatggctgac gaattcggtt
    14221 ccccgtcggt ggatctcgac aagctcgatc agccgggtgc gggcagcacg ctggctgagc
    14281 ttgacgaagc cctcgcccac ctcaatctgc cgctgcgccc cgagtcgctg cccgtgccca
    14341 ctaccgacgc ccacacccac gccggtagta caacggagta cagcaagctc tcgcttgagc
    14401 acatccttgc cgctgctcgt tcggtgaacg taacccggat cgtcgaagtg ggcaccgacg
    14461 ttgtctcttc gcggcaagcg atcgctctgg ctgacaccca gcctggtgtg gtggccgcgg
    14521 tcgcgattca ccccaacgat gcggcgcggc tcggcgcgcg gctgccggcc gaactcgccg
    14581 ccctggaagc gttggtgggc tcgtcgaccc gcgtccgcgg cgtgggtgag acgggactcg
    14641 actacttccg caccaaggac ccggccggcc aggcgctgca gaaggaagcc ttcgccgcgc
    14701 acatcgcctg ggctaagacg cacgatctgg cgctggtgat ccacgaccgc gatgcccatg
    14761 ctgacatcct tgacgtgctg gccgctgagg gtgcgccgga ccgggtgcag atgcactgct
    14821 tctccggcga cgccgagttc gcccgggcct gcctgggccg gggttactgg ctgtcgttcc
    14881 cgggcacagt caccttcaag ccgaatgagg ccctccgcca ggcgctcgat ctcacgccgc
    14941 cggacaagtt gctggtcgag accgacgcgc cctatctcac cccgctgccg tatcgcggcc
    15001 gggcgaactc gagctatctg ctgccgcata ccgtccgctt tatcgccgag cgccgaggcg
    15061 tcgacctggc cgaactctgt gcgcaactgg cgacgaacgc gtccgctgtg ttcggggatt
    15121 ggggcgatgg tgacgcctga gcgcggatca acccactttc gacaggttat gaaggctcga
    15181 ttcggcgcct gcgcgcgacc aacccatttt ctgcaggcta tgcgggtcga aatgggctcc
    15241 gtaacctgta gaaagtgggt tggattcgag tgcaactcgg gatcggccct cataacctgt
    15301 ccgaattggg ttcaactgca aagaaccgcg ctcggtaatg ccatcgcaga ggagcggcat
    15361 ggctgacgga cttctcgacc cgcgcacgat ccgggagctg gccgccgagc tcgacctgcg
    15421 gcccaccaag cagcgcggcc agaacttcgt gcacgatccc aacacggtgc gccggatcgt
    15481 cggcgctgcc gggctcggtg ccgaagacgt cgtgcttgag gtcggtcccg gtttgggatc
    15541 gctgacgctg ggcttgctgg agcaggtgtc gcaggtgatc gccattgaga tcgagcccag
    15601 cctcgccgcc cggcttccgc ggacggccgc tcagcggctg cctgcacgcg ccgaccgcct
    15661 tcgggtgatc gaagcggacg cgctcacggt cgatgtcatc cccgacccac aaccgaccgc
    15721 ggtggtcgcc aacctcccct acaacgtgag cgtcccggtt ctgctgcatg tgctggcccg
    15781 cttcccgggc attcgcagcg gcctggtgat ggtccaggcc gaggtggctg accggctggt
    15841 cgccccgccg ggctctaaga cctacggcat cccttcggtg aagctcgcgt ggtacgccgc
    15901 tgctcgccgg gtgggcagcg tgccgccgaa ggtcttctgg ccggtgccca acgtcgactc
    15961 ggggttggtc gaactgatcc gccgcgaccc gccgaccaca accgcgtcca gggagcaggt
    16021 gttcgcggtg gtcgataccg ccttcgccca gcgccgcaag atgctgcgtt cggcgctgtc
    16081 cgggctgttc ggctcctcgg ctgcggcgtc cgctgcgctg gaaggtgccg gcgttgatcc
    16141 gcaggcgcgc ggcgaggtgc tgggcgtgtc cgatttcgtg cgaatcgcgg aagctctcgc
    16201 cgataatccg cgtgggctag cctcagtcca tggcatcccc ggtgaccgag actgaagccg
    16261 tccgcgtgcg cgtggcgggc aaagtcaacc tcgccctgcg ctccggtcca cgacgcccgg
    16321 acggctacca cagcctggcc accgtctttc aagcagtgtc ggtcttcgat gaggtcgagg
    16381 ccacgtgggc gaagcctgga caaatcaccg tcagcgtggt gggtgaacag gccgatctgg
    16441 tgccgtccgg ggagtcgaac ctcgcagtga aggcggctcg gctgctcgcg gcaacagccg
    16501 gcgcagggca gccgctgggc gccgaactgc tggttcgcaa ggccattccg gtgactggag
    16561 gcatggctgg tggttccgcg gacgcggctg gcgcgctgct ggcgtgcgct gtgctctggg
    16621 atcttgacgt gaccccggac gagcttcgcg agctgggcgc ccagctgggc gcggacgtcc
    16681 cgttctgcct ggccggtggc accgcgctgg gcaccggacg cggcgaccag cttgccccag
    16741 tactgagtcg gggcacctac cactgggtgc tcgccttcaa cgaggccgag ctgtcgacgc
    16801 cagccgtgtt cgccaagttc gacgagctgg ggctggccac cgagccgctc acggttccgt
    16861 ccgagctgct gaatgccttg gtcagcggcg atgtgcaggc gctcggccgg acgctggtca
    16921 acgacctgga gccggcggcg ctggcgctgc ggccggagct ccgccaggtg cttgacgccg
    16981 gccgcgaact aggcgcgatc gccgcgatgg tgtcgggttc cggcccgacc gtggcgttcc
    17041 ttgcgccgaa cgagccggcc gcggtcgact tgtcggtgaa gctctccggg gcgggactgt
    17101 gccgcgccgt gaagcgggtg tccggaccag ttccaggtgc ccgcctggtc gtctagcgtc
    17161 ggcaggccga gggatggtcg acccctccac ttgtcatccc ggcgcaggcc gggatctccg
    17221 tgctgccgcc gaggccgcac gccgcctacc tggcgtccga gagctcctcg accgctacct
    17281 cacggcccgg catgatccgg gccaccagca gcagacccag cagcagcgcg gccggcacga
    17341 acatggtgtg ctggatgccg atggtctgca ccagcatgcc gacgagtgcg ggcgcgatca
    17401 ggaaggtggc gtagccgaag gtgacgacca ccgcgagacc gcgtccgcca gcggtgtggc
    17461 cggccactgc gtagacctga ggggcgatca ttccgatccc aagtccgacc agcgaccagc
    17521 cgatcagcag caccggcagc gtcgcgccag tggcagcgat caggtagccg agcgccgagc
    17581 aggcgccacc gaagccggtg accgcacggc gtccgaatcg ggtgaccagg aagtcgccga
    17641 agagccggat caccaccatg aatgcggcca cgcaggtgaa gccaagggcg ccggtggtcg
    17701 cgtccacgcc ggcgatctgg gtgacgtgca gcccagacca gtcgttggcg gtgccctcgc
    17761 cgaggccgaa ggcgatcgcc atcaggccga ggaggtacgc cgagcggggg atcggggtct
    17821 tggcgccggt ctcgtcagcg tggctgatca cggcggtctc gggtgtgatc cgccaggcga
    17881 ctccgagcgc gaccaaaccg agtgcggacg cgatcgcggc agcggcggca actgtcggct
    17941 ccgggttgcg ggcgaaaaac ggcgccagtc cgacgataag ggcggcgccg acgaggttgc
    18001 cgaccgacca catgccgtgg aagaagctca tgatcggtct gggacggttc tgctcgactt
    18061 ggacgccgac cgcgttcatg gccacgtcga tgccaccgtt gcccagtccg agcaggatcg
    18121 cggacgcgac cagcaccggc agcgtggggg cgagtccgat gccgaggatg ccgatcgcca
    18181 gcaccgggat catcgccagg ctgatccgac gggcaccgaa acgatcggcg agccgtcccg
    18241 atgcctgcat ggacgcgatg gcggccaggc cgatcgcgac gaacagcatc gacaggtgga
    18301 ttggggccag gttgaagcgc agcaccagcg tcggaatggc cgcggcgtag acgccgatca
    18361 ccagcccgtt gtagaagaag agcaggacga cggcgagtgc agccttgggc gagccgagcg
    18421 agatcacccg ctcactctat ggtcgaaggg cagattgtga aaacgctctc aggtggctgg
    18481 gctccgatcg cttccagctg ttgccgtggc gcggtggtga gcgtcaggaa tcgcgctcgg
    18541 cccgctcccg cacgtagagc gtgggattcg gtcgcagatt gcgcatgccg taccaagcca
    18601 ggttcaccag gtgcgcggcg accacgggct tgtcgagttg ctgctcctcg cgagtgtcca
    18661 tccaccactg accggcgagc gccacgaggc cgaccagcat ctgggcgtac atcggggcgg
    18721 tctgggcgtc cagctcgcgc ttggtgaact cgttggccag aaggccctcg acccgcgagg
    18781 caatgtcgga gaggatcgtg gcgaaagagc cttcggggga ggtggccgag gagtccctgg
    18841 agatgatccg gaacccgttc gggtggtcat cgatgtagtc gagaagggcg agcgcgccca
    18901 gttcgatgag cgcgcgaggg ttggctcgcg gggtggtgag ggcggtgcgg atggcgtcgt
    18961 ggagcgtccg tacttcgcgg tcgaccacga cggcgtaaag gccctccttg ccgccgaaat
    19021 gctcgtacac cacgggcttg ctcaccccgg cgtgcgcggc gatctcctcg accgaagtgc
    19081 cttccaggcc gcgctcggcg aacagcgctc tggctacgtc aatcagctgt tcgcgacgct
    19141 ccgctctggt catgcggacg cggctgcgcc gggcacgaga ggtggccgcg gctgaggaca
    19201 tggcccaagt ctggccccgt cagcgcggga tcgccaatcg cttgggccaa ctggccgaac
    19261 cggtgtgagc tagtcagtgt gacgatttgc actggcgatg acgggaagcg cgaatgcggc
    19321 acgatttacg agcatcggca gggcaaatcg tcacgggcag gcctgacgtg gtcggggaac
    19381 aagcatctag cggcgcggtc gcgggatgca tccaccccgc gccgacccgt agagttgacc
    19441 ccggccgtcc gcggacggcc gttccccggt tggtctgccg gcagggcccg cctgactttg
    19501 aatcaggact agcgacgtag gttcgactcc tacccgggga gcttcggagg attcgtacca
    19561 ttgagattct gccgagatcg aaactgagaa tctgccgact aggccacgga ctcgatagtt
    19621 acccggttcc agctcaccgg cgtcgccgcg cgatcaatac gaacaagcca gagacaagca
    19681 tccgcaccac gatgttcgcg atcagcactc cgctcactcc aatcgcggcg agagggatga
    19741 cttccaggac agcagtggcg ccatggaagg cggcgaagct cagcgcgatc agccggatcg
    19801 cgtccgggtc acgaagccgg gcggccccga gtgagagcag gccgatgccc agctcgggcg
    19861 cggccagaac gtagggcagc aagtactcgc tgcgcgcgag cgcgatgccc acggtcgcgg
    19921 ggatggccgc gggaaacacc accagcacta tcgccgccgc cagcatgatc acgccgtgcg
    19981 cgatgaagat gacgcgctcg gtccgtggtg tcaccgccca agcgtcccac cgccggccgt
    20041 ccctgtgatc gtggctatcg cgggattcgc ccacccgccc gacctgtgag cagacccgcg
    20101 agactcggta gggtgtaacc gcgcttccca cggcgcgtcg atccggcgaa gcccgccgct
    20161 aggtgcccgg ggagcggata tgcaaccaga ccttgggagt gcccgtggcc gagcaggacg
    20221 cagttgtgga caaccgggtg gcagcagttg tcatcatggc ggccggagcc ggcacccgaa
    20281 tgcgatcgtc agtcccgaaa gtgctgcacc cgatagcggg caagtcgatg atcgcgctgg
    20341 cgatcgacac cgcggacgcg ctggctcccg agcgactagt cgtggtcgtc ggccaccagc
    20401 gcgagcaggt gaccgcacac ctcgcggaag tcgccccgca cgtcacgatc gccgtccagg
    20461 cacagctcct gggcaccggc gacgccgtcc gcgccggcct ggccgcgctg cccggcatct
    20521 ccggcgcggt ggtcgtcacg tccggcgacg tcccgctgct cgccggtgag accctgcagg
    20581 gactagtcgc cacgcaccgc agcgagggca acgcgatcac cgtcctcacc tcgatcgtgc
    20641 ccgacccgac cggctacgga cgcatcgtcc gcgagggcga ccacgtggcc cgcatcgtcg
    20701 aacagaagga cgcgagcccg gacgaactcg acctcaacga gatcaactcc ggcatctacg
    20761 tcttcgaggc cgatgtgctc ggcgaaggca tcgaagccct ggaagccaag aacgcccagg
    20821 gcgagctgta cctcaccgac gtgatcgcct acgcccgcca ggccggacgc cgggtgggtg
    20881 cccagctgct tgccgaccac ctgcagaccg agggcgtcaa cgaccgcatt caactcgccg
    20941 agcgcggcgc ggagctgaac cgccgcatcc tgaaccgctg gatggccgcg ggtgtcaccg
    21001 tgatcgaccc ggccaccacc tgggtgcacg ccggcgtcga cctggccgag gatgtcaccc
    21061 tcctgccagg cacctcgctc gaaggagcaa cctccgtagc cagcggcgcg atcatcgggc
    21121 cggagacgac cctgatcgac gttgaggtcg gcgagaacgc ccgcgtgctg cgcacccacg
    21181 ccgaacttgt cgtgatcggg cctgaggcca ccgtcgggcc gttcacctac atgcgtccgg
    21241 gcaccgagct ggccgctcgc gtccacgtcg gcgcgttcgt cgaaaccaag aaggcgatca
    21301 tcggtgaggg caccaaggtg ccgcacctca cctactgcgg ggacgcgcgg atcggtgcgg
    21361 gcagcaacat cggtgccggc acgatcttcg ccaactacga cggggtgggg aaatacacca
    21421 cgaccgtcgg ttcgcactcc ttcgtcggca gcgattcggt gctggtcgca cccgtcgaga
    21481 tcgcggacgg cgcgtacgtc gcagccggct cgaccatcac cggcgacgtc ggtccgggcg
    21541 agttggccgt cgcccgcgga cagcagcgca atatcgccgg ctgggtcgcg cggaaacgtg
    21601 cgggcaccaa atcggcgagt gcggccgcag ccgctctggc cgccaaggcg gaggctccgg
    21661 gggcttccga gtgagcggca tcaagcgtcc gaccgaaaag cacctcatgc tttgcaccgg
    21721 acgggccttc cccgaactcg ccgaggacat cgccaagctg ctgggcgtcg acctggtgcc
    21781 gacccgctcg ctcacctacg caaactccga gatctacgtg cgtttcgagg agtcggtgcg
    21841 cggtgcggac gcattcgtga tccagtcgca ccctgcgccg gtgaacgagt ggctgatgga
    21901 gcagttgatc atgatcgacg cgctcaagcg ggcctcagcc aagcgcatca ccgtggtctc
    21961 gccgttctac ccctacggcc gccaggacaa gaagcacgcc ggacgcgagc caatctcggc
    22021 ccggctcgtc gccgacctct acaagaccgc gggcgctaac cggctgatgt cgatcgacct
    22081 gcacgccgcg cagatccagg gcttcttcga cggcccggtc gaccacctgt tggcgctgcc
    22141 ggtgttggtc aagtacgtga aagagaacta cgacgtctcc gagatgaccg tggtctcccc
    22201 ggacgccggt cgggtgcgcc tggccgacat gtggtccgac caactgggca ccccgctagc
    22261 gatcatccat aagcgccgcg acccgaacaa ggccaacgag gtcgcggtcg gcgaggttgt
    22321 cggcgacgtg aacggacggg tctgcctact ggtcgacgac atgatcgata ccgctgggac
    22381 gatctgccag gccgcggcag cgttgaaggc gcacggagcg aaggcggtga tcgccgccgc
    22441 gacccacccg atcctttccg ggcctgcccc gcagcggctg aacgagtccg agttcgaaga
    22501 ggtcatcgtc accaacacac tgccgatcag cgagggcgtc aacatcccca agctgacggt
    22561 gctctcgatg gcgccgctga tcgcgcaggc gatccaggcc gtgttcgagg acggctcggt
    22621 cgcgtccctg ttcaacggcg cccactgagc ccgcgtccgc tcgattcgtc cggatttctc
    22681 ccgacgtgag cggacgcgat aagctttccg ggttgcctcg gcgaggggcc cggcgaggac
    22741 gccaacggcc cgtgatcgac aaggcggagc atgtgcgccg ctgatcactc cctcgcccag
    22801 gattggtaaa tggatgaggt tgaggagcaa gagcaagtga ctgacagcaa aatcgcagca
    22861 gtgacccgta ccgagttcgg caagggcgcg gcgcggcgca cccggcgggc tggactcgtt
    22921 cccgccgtcc tgtacggcca cggcaccgat ccggtgcacc tgtcgctgcc cggccacgag
    22981 gtgcagctcg cactgcgcgt cgccaacgcg gtgctggagc tgtccgtcga cggtggcaag
    23041 acggagctgg cgctggccaa gcaggcccag cggaacccga tcaagggcaa cgtcgagcac
    23101 cttgacctgg tgatcgtgaa gaagggcgag aaggtcaccg tcgaggtgcc gctgctcgtc
    23161 gtcggcgacc acccggccga ccgcatgatc gtcatggatc agcagaccat ctccctcgag
    23221 gtcgaggcga cccacatccc gaccggtgtc gagatcaaca tcgccggcct cgagatcggc
    23281 gacaccgtca ccgccaagga cctggtgctg cccaagggcg ccgtgttccc gggcgagccg
    23341 gacgacctga tcctctccgt cgccgccacc ccgagccagg ctgcgctgga cgctgaactg
    23401 gctggtactg acgaaggcgc cgaagccgag gccgccgaag aggcgtgatc tggcttcatg
    23461 gctgattggc tggtggtcgg gctcggtaat ccgggcccga cctacgcatc tcaccggcac
    23521 aatgtcggtt tcatggtggt ggacgagttg gcgcggcgcg ccggcgtccg cttctcccgg
    23581 tcgctaacgc tgcgcgcgga gaccgcccag acccgcatca ccgcgtccgg tggtatcggt
    23641 ggcatcggtg cggacgccga ccgggtggta ctgctgaagt cgcgcacctt catgaacgaa
    23701 tccggtatcg cggtgtcgaa agccgcgaac tacttcaaga cccccgccga ccacctggtg
    23761 gtggtgcacg acgaactcga tctcgatttc ggacgcatcc ggctgaagtt cggcggcggc
    23821 gacaacggcc acaacggcct taagtcgatc cgccggcacc tcggcaccgg cgacttcttc
    23881 cgggcgcgct acggggtggg gcgtccgccc ggccgccagg tcgcagctga ctatgtgctc
    23941 agcccgttcc cgtccgcgct gagcgaggac gtggcggtag aggtctcccg ctgtgcggac
    24001 gcggtcgaat cgctgctcac ccaggggctg gacgcgaccc agggtcgctt caactcctga
    24061 ccgttcagtt ggtgtcggat gccgcgagta gggtgatgcg gtgattcttg acggcttgat
    24121 cgaactggtc ggcagcgagc cggtgatcgc ggaggccatc gccgacagcg acaccggacg
    24181 gctgcccgcg ctcgatctga ccatgccgga gccgctgcgg ccggtgttca tcgccgccct
    24241 cgctcgccgg accagtcgga cgatcctgct ggtcacgtcc actttccggg aggcggaagc
    24301 actaaccagc gcctgcactt cgctgctgga cgagcatgac gtcgcctact acccggcctg
    24361 ggagaccctg ccgcacgaac ggctcagccc gcgctcggac acggtcggac gccggctcgc
    24421 ggtgctgcgt cggctggccg gaaacgacgt gctgccggct ccgcgggtgg tgatcgcacc
    24481 ggtgcgttcg gtgctgcagc cgcaggtggt cgggctcggc aacctgctgc cggtgacgct
    24541 ggtgaccggc gaggactacg gactggagga cgtcgcccgc ggcctggtcg acgccgccta
    24601 catccgggtc gacctggtcg aacgacgggg cgagttctgc gtccgcggcg gcatcatcga
    24661 catcttcccg ccaacgcttg aacacccggt acgcgtcgac ttcttcggcg acaccgtcga
    24721 ggagatccgc ccgttcagcg tcgccgacca gcgctccttc gacgaaaccc tgcccgaagt
    24781 cgtcgcatca ccttgtcgcg agctgctgat caccgaatcg gtgaaggccc gcgcggccga
    24841 gcttgcccgc acccatctca acgctcccgg caacctgggc gagctcttcg acaagatcgc
    24901 ctccgggcac gcggtggacg gcgtcgaagc gctcgcgccg gtcctggtgg acggactcga
    24961 actgttgatc gacgtgctgc ccgccgacac ccagatcctg ctgtccgacc ccgagcgcat
    25021 ccgcgccaga gcccacgagc tggtgatcac cagtgaggag ttcctgcgcg cctcctgggc
    25081 cgccgcggcc gagggcggcg tggcgccgat cgacctggga gcgtccgcgt atcgctcgct
    25141 ggcggacgtc cgcgcgcacg cgcttgagct gggccaggcc tggtggagtc tttcgccctt
    25201 cagcgccggt ccggacgaga gtgcggacgg gatatcaccg gccaccgtcg aatcagcgac
    25261 ggtatccgcc tgggacgtga gcgcggtccc gtctcgcacg gtccaggcca cggaggcgcc
    25321 ggtctggcgc ggcgacaccg aggccgcggt cgcgtccatc cgggaatccg cagccgcgaa
    25381 gcaccggacg atcctggtgg ccgactccag cgggctcgcg gcccgcctgg tcgagatcct
    25441 caagtccgcc gacatcccgg ccagccgtcc cgaacagctc gccgaaaccc ccgaaccggg
    25501 cacggtcacc gtggtcaggg ccgagttgcg gcacggcttc tcacttcccg gcagcgatct
    25561 ggacgtgttc accgccggcg acctctccgg ccagcgcgag gccgacaagg cgtcccgcaa
    25621 gatgcccgcc cgccggaaga accagatcga tccgctggag ctgaaatccg gcgactcggt
    25681 ggttcacgaa acccatggcg ttggccgcta cgtcgaaatg gtgcagcgga cggtcgccgg
    25741 cgccacccgc gagtacctgg tcctcgacta tgcgtcgtcc aagcgcggcc agccggcgga
    25801 ccggttgttc gtcccgatgg accagctgca actggtcacc cgttatgtgg gtggcgagaa
    25861 tccgaccctc gaccggctcg gcggtgccga ctggaagaac cgcaagcaac gggcccgcaa
    25921 ggccgtccgg cagatcgcgg ccgaactgat caagctttac gcggcccggc aggcgtcccg
    25981 cggccacgcg ttcgctcccg acacggtctg gcagaacgaa ctcgaggatg cgttcgccta
    26041 cgtcgagacg cctgaccagc tgtcggcgat caccgaggtc aaggccgaca tggagcaggt
    26101 cgtgccgatg gatcggctga tctgcggcga cgtgggctac ggcaagaccg agatcgccgt
    26161 ccgcgctgct ttcaaggcgg tgatggacgg caaacaggtg gcggtgctgg tgccgacgac
    26221 cctgctggtg cagcagcacc acgccacctt cgccgaccgc tatgccggct tcccggtgac
    26281 cgtgtccgcg ctcagccgct tccagaccga agcggagaag aagaaggtgg tcgccgggct
    26341 ggccgacggc ggcatcgacg tcgtggtggg cacgcaccgg ctgttctccg accagatcga
    26401 tttcaaagat ctcggtctgg tgatcattga cgaggagcag cgcttcggcg tcgagcacaa
    26461 ggaggcgctg aagaagctgc gactgaacgt cgacgtgctc tcgatgagcg ccacgccgat
    26521 tccgcggacg ctggaaatgg ccatcaccgg cattcgcgag atgagcgtga tcgccacccc
    26581 gccggaggag cggcaccccg tgctcacctt cgccggaccc tacgatgccg gccaggtgcg
    26641 ggcagcgatc cggcgcgaac tggcgcgcga aggtcaggcg ttctatatcc acaaccgggt
    26701 gcagtcgatc gagaagaccg cccagcagat cgccgaactg gtgccagaag cgcgagtcgc
    26761 ggtcgcccac ggccagatga acgagcacac cctcgaacag gtgatggtcg acttctggga
    26821 gcgccgcttc gacgtgctgg tctgcaccac gatcgtcgag gccggcctgg acatctccac
    26881 cgccaacacc ctgctgatcg agcgcgccga cctgctcggc ctctcccagc tgcaccagct
    26941 tcgcggacgg gtcggacgcg gccgcgagcg tggctacgcc tacttcctgt atccgccgga
    27001 gaagccgctc accgagaccg cccacgaccg gttggcgaca atggccgcac acaccgacct
    27061 cggcgcgggc atggcgattg cgatgaagga cctggagatc cgcggcgccg gcaatctgct
    27121 gggcggcgaa cagtccggtc acatcgccga agtgggtttc gacctctaca tccggctggt
    27181 cggtgaagcc gtcgcggagt tccgcggaga ggacaccgag cccgagccgg agatgaagat
    27241 cgagctgccg gtggacgcgc acctgcccgg cgactacgtc tcgtccgagc ggctgcggct
    27301 ggagatgtac aaacggctgg ccgcggtcac gtccgcagcc gacatcgccg ccgtccgcga
    27361 ggaactggtc gatcgctacg gcgaaccgcc ggcgccggcc gaggcgctgc tcgcggtggc
    27421 cgcgttccgg ctggccgcgc gggcgcacgg agtgaacgag gtggtcgcgc aagggaacat
    27481 gatccggttc gcgccggccg ttctgccgga ttcggcgcag ttgcggctct cccggctcta
    27541 cccgggctcg caggtgcgca aggcgaccga gctgatcctg gtgccgcggc cgatgacccg
    27601 tccgatcggc ggccagccga tcaccgacgc gcccctcctg gaatgggcga gcaaggtgct
    27661 gagcgacgtc ttcaccgtcc ccgccagccg ataggatcgc cgatggcgca cgaaacgaaa
    27721 gtgggacaga tgcacaaggc agccttcaag ctcgcaggac tggtcgccgg gacggtggcc
    27781 ctcgtcggtt gcgcgggcgg gaacccgaca gtggcggcct atgtcggggc ggagaccatc
    27841 tcgcaggcca aggtggacgg ggtcgcgcgc gtgctcgccg acgccacagc cgatgcgacc
    27901 gacaccgccg gtggctacgg ccagaccgtg gtggcgatcc tggtgcagtc gaagctcgcc
    27961 gcccaggccg ccgcgacgaa gggcatcacg gtctccgacg ctgagcgcca gcaggtctac
    28021 gacagcaacg agctgtacac cgtgctgctg aacaacccgg cgaccgccga cttcatgcgc
    28081 gagtacgcga acaccgcggt catcttctcc accgaggaag gcaaggccgg cgtcaacgag
    28141 ctgctcggca agaccacgat cacggtgaat ccgcgcctcg gcacctggga cgccgcaatg
    28201 ggcggcgtcg tccagggcag caccggatcg ctttccgaac tggccagcgc gaagtagtcg
    28261 acgtggcgca gccacagctc gagcggctgg tcgccgtgat gcaccggctg cgctcgggtt
    28321 gcccctggga cgcggagcag acccacctct cgctggtgcg ctacctggtc gaggagaccc
    28381 ttgaggtggt cgaggctatt gaggcgggcg acgacgacca gctcaccgag gaactcggcg
    28441 acctgctgtt gcaggtggtg ttccacgccg aaatcgcagc cgagtcccgc cgtttcgaca
    28501 ttgaggatgt ggctcggcga atcgcggaca agctggtgtc ccggcatccg tacgtgttcg
    28561 ccgaggcgga cgtgccgtcc gacctggtca gctcgtggga gcgggcgaag gcagtggaga
    28621 agggccgcga ttcggcattg gacgggattc cggagcgcct gtccgcgctg accagggccc
    28681 agaaggtgct cggccgctcg cgcagccacg gactcgatct cgcttccttg ggcgtcgagc
    28741 cggccgatca ggccgccccg gaacttggca ccgcgttcct gagcctggtc gccagagccg
    28801 acgagcttgg cctggacgcc gagcaggccg cacgctcggc ccttcgcgac ctggagtccc
    28861 gcgtccgcgt agccgaagcc aactctcgct gatacccgcg aacggtccct gaggaggctg
    28921 gtgaggcacg agccagccgc ccgcgttggg tcaccgcgac aacacattgg acgcagcgtc
    28981 cacccgcacc caccgtcatc cgctcgcacc caccgtcatc cgctcgcacc caccgtcatc
    29041 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    29101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncacccaccg tcatccgctc
    29161 gcacccaccg tcatccgctc gcacccaccg tcatccgctc gcacccaccg tcatccactc
    29221 gcacccaccg tcatccactc gcacccaccc gtcatcccgg cgcaggccgg gaactgacca
    29281 agacatcgac gtggcgtcca gtaacttccg gcgggctgcc cccgagggcg gtcaacgcta
    29341 gggtagaggc gaacacaatt ccgcttgaaa ggcacacacg tggcacttat cgaattcgtt
    29401 catgcgaggc agatcctcga ctcgcgtggc aaccccaccg ttgaggtcca ggtcgtgctc
    29461 gacgacgaca ccgagggccg cgctgcggtc ccgtccggcg cttcgaccgg tgcgttcgaa
    29521 gcagccgaac tccgggacga ggacgctgcc cactacggcg gcaagggcgt gctgaaggcc
    29581 gtcgagaacg tcgtggagca gatcgctccc gaggtgatcg gcatggaggc ctccgagcag
    29641 cggctgctgg accaggccat gatcgagctg gacggcaccg acaacaagtc caagctgggc
    29701 gccaacgcga tcctgggcgt gtccctggcc gtggcgcagg ccgctgccga gtccgccggc
    29761 ctgccgctgt accgctacct cggtggcccg accgccagca tcctgccggt gccgatgatg
    29821 aacatcctca acggtggctc gcacgccgac tccaacgttg acatccagga gttcatgatc
    29881 gccccgatcg gcgcggcgac cttcgcggac gccctcgaaa tgggcgccgg cgtctaccac
    29941 gcgctgaagg cggtgctgaa gggccgcggc ctgtccaccg gcctcggcga cgagggcggc
    30001 ttcgccccga acctggagag caacgccgcc gcgctcgacc tcatcctcga ggccatcggc
    30061 aacgccggct tcgtcccggg caaggatgtc gcgctcgcgc tcgacgtggc cgcgtccgag
    30121 ttctacaacg aggacggcac ctacaacttc gagggcggca agaagaccgc tgccgagatg
    30181 accgcctact acgccgagct ggtcgccaag tacccgctgg tctcgatcga ggacccgctg
    30241 aacgaggacg actgggacgg ctggaagacc atcaccgatg agcttggtga caaggtgcag
    30301 ctggttggcg acgacctgtt cgtgaccaac gtctcccgcc tgcagaaggg catcgacacc
    30361 cagactgcca atgcgctgct ggtgaaggtg aaccagatcg gctcgctgac cgagaccatc
    30421 gacgcggtcg acatggctca ccgcgccggc tacaagacga tgatgagcca ccgttccggc
    30481 gagaccgagg acgtcaccat tgccgacctc gcggtcgcgc tcggctgtgg ccagatcaag
    30541 tccggcgccc cggctcgttc ggagcgcgtg gccaagtaca accagctgct gcgcatcgag
    30601 gaggacctgg acgacgccgc gcgctacgcc ggtgcgtccg ccttcccgcg gttcaaggcc
    30661 tgattgacac ctgtcccgtc ggagattccg gcctgcgccg gaatgacggt gacctggcgt
    30721 gcgagcgcgt cggcggattc cgccggcgcg ctttgccgtc cttgggagcg gtcccgcgcg
    30781 tccgcgtggc gcgccgcgag ggcccgccta tccttgacca gatgagaatc gagggccatg
    30841 gctcgcggca cgcgtagccc gcgcaacgac accgccggcc ccggacgccc cacgcggcgc
    30901 acccgggccg gcagccgtgc gtccggtccg gacacgccga gcgagccgcc accgcgggat
    30961 cgcagggacg aggtgccggt ctggcgccgt ggcctccacg tcacccggcg cgccatcgtc
    31021 ctgatcctgg tggtcgtggc cctggtcatc tccttcggtg gttcgctgcg cggctatctc
    31081 gcccagcagc acgaactggc ggtggctgag caggagatcc gcgatcgctc ggcgcagatc
    31141 gccgaccttg aggccgaact ggcccgctgg gacgatccgg actacgtcaa ggcgcaggca
    31201 cgcgaccgtc tcggctgggt gatgccaggg gagaccggct atcgcgtgat cggcgcggac
    31261 ggcaagccga tcggtggcgg agtggtgatc gaatcggccg agaaactgcc ggccggcgag
    31321 cacgaaccgg tctggtggga tcggctctgg ggctcgatgc aggccgccga cgcgcctgct
    31381 cgcaaggtca cctcgccgta gcaaaccgga aaccgggcgg cagccatgac gggatatcgg
    31441 agccgtggcg tcagcccgag tgacggggca gcgtcaggac ggatccgctg ccgagatcgc
    31501 gacccggtgc agcctcgaca ggcttgacct cccgaactcc gtcgacggtc tcaatcaccc
    31561 gagcttcacc catgggagaa tgtccggcgt gccgagcttt gaacccctga cccaatcgga
    31621 cgtggccgcg gtcaccgccc aactcggacg cgaacctcgc ggctcggccg gggtggcctg
    31681 gcgttgtccc tgcggtaagc cgggcgtggt ggccaccaac ccgcggctgc cggacggcac
    31741 cccgttcccc acgacctact acctgacctg tccgcgcgcc actgcgggct gttccacgct
    31801 ggaagccgaa gggctgatgg ccgacatgac cgagcggctc accagcgatc ccgaactggc
    31861 gtccggctac accgccgccc acgaggcgta cctcgctgat cgcgccgaac tcggcgatgt
    31921 gaccgagatt gccggcatca gcgccggtgg catgccgtcc cgggtgaagt gcctgcacgt
    31981 gctcgccgcg cactcgctgg ccgcgggccc gggtgtgaac ccgctgggcg acgaaactct
    32041 gacaaggctt ggcgagttct ggtcgcggcc gtgcctcagc gccaccgacc ccgaaatcag
    32101 ccccgaccag cccggacgcg agccggagcc cgcggatcgt ggctgagctc ttcgcggcaa
    32161 tcgactgcgg caccaactcg gttcggctgc tgatcacccg cttggtggac ggccgccttg
    32221 aggaggtgga tcgtcggctc cacatcactc gactcggtca gggtgtggac gccaccggcg
    32281 ccttccaccc cgacgcgctc gcccgcactc tcgacgccat ggccgacttc ggtgctcagc
    32341 tggacgggtc gggcgtccag catcgccggg tggtggccac gtccgcagcc cgggatgcgg
    32401 ccaactcggc cgagttcttc gaaggtgtaa aggcacgggt cggagtgccg gccgagatca
    32461 tctccggcgc ggaggaggcg tacctgtcgt tcaccggtgc ggtcaccgcg ctgccgcacc
    32521 tgccgcagcc ggtcctggtc atggacatcg gtggcggctc gacggaattg gtggtgggct
    32581 caccgcacgg cacggacgtc gcgatctcgc tgaacatcgg ctcggtgcgg ctgcgggaga
    32641 gccacctgca ttccgacccg ccgtccgcgg ctgaggttgc gaccgcgtcc gtccgcatcg
    32701 acgaactgct ggacgacagc ggcatcgact tcaggatggc agccagttgg gtgggcgttg
    32761 gcggcaccgt gaccagcctc tcggcgctgg cgcagggcct ccacacctat gaccggacgc
    32821 gtgtgcacgg ctccgtgcta tctcagccag ctctttcagc cctcaccgcg cgcctgctcg
    32881 cgatgccgat cgccgaggtc gcagcactgc ccaccatggt gcccggacgc gcggacgtga
    32941 tctgcgccgg cgccctgatc tgcgcccgcg tcggcgcacg catgtcgcgg accgacttga
    33001 ccgtgagcga ggccgacatt ctcgacggaa tcatcgccag attggctacc gaagccggaa
    33061 tgtgagcgtt acgcagcgaa ctgcaagaag cgccaaatag gtggccagtc gcgcttgata
    33121 acgatgcggt aacttctagg ggaaactgat tgttagggtt cacatgtgag ccctaacagc
    33181 ttgcgtacgg ccaagggcgg ccgtccggtc cggagagtct ccctcccgtc cagggacgca
    33241 caaggaggtt tcacacgtga agaagaccct tggtctctgt ccggccacgc gctcaccgcg
    33301 gtgggtaccg agatggacgc gatcgcggcc gtggtgatcg gcggcacgct gctgaccggt
    33361 ggttacggct tcgtgctggg ttcggcgctg ggcgtactgg tgctgggcct aatacagacg
    33421 atcatcagct tcgagggcac tttgagttcg tggtggacga agatcttcat cgggggactc
    33481 ctgctggtgt tcatcatcct gcagaaggtg ctcacatcca ggagaacttg agcgatcgag
    33541 gacgggagca tcagcgcccg agggatccac cacgaggaag ccagcgggcg tgcacgagga
    33601 cgaacgcgca agagccgcga cgatcttcga tgtcgcgcgc ctggggggtg tcccaccaga
    33661 ctgtctccag ggtgctcaac gagctggcga acgtccgtcc ggccaccggg agcgggtgga
    33721 gcgagcgatc aagcagttgc gctacatccc gtccccggct gctcgggcgc tggtcacccg
    33781 gcgttcgcgg acgatcgggc tgatcaccac cgactcgccc gacttcggcc cttcgacctc
    33841 cgctgtgcat atcaacgagg cggcgcgcga cgagcgctat gcggtgatca tggcgagcct
    33901 ccgccgaacg gacgcgccgg cgctgcgtca ggccgctgag gtgctggtgc ggcagaacgt
    33961 ggaggcgatc atcctgatca cgtccagtcg ttcggtgctc ggcatcctgc agggcatgga
    34021 gctgggcgtg ccctttcttg cggtggcgtc gcagggctcc gggctgcatc gggtgtcgct
    34081 cgaccagtac cagggcgccc ggcgtgcggt tcggcacctg attgagctgg ggcacaccga
    34141 catccgccac gtgagcggac cttccgactc gatggacgcg atcgaacgca tccgcggttg
    34201 gcgcgacgcc ctcggcgagc acggcctggt ggcccatgaa ccgttacccg gggattggtc
    34261 gcccgacagt ggttatccgg ccggacggca cctgctcaac gagacgttga cggccgtgtt
    34321 ctgtgccaac gaccagatgg cgctcggcct gatgcacgcg ctcaccgagg cgggccggtc
    34381 cattccggga caggtgagcg tggtgggctt cgacgttatt cccgaagcgg cgcacttctc
    34441 gccccccgct gaccaccatg caccaggact ttgcgcaagt gggcaggggc gcgatggagc
    34501 tgatgctagc tgtgctcaac gactacgaca ccccaacccg ctgctccggt tgccgcagtt
    34561 gatcgagcgc ggaagcgcgg cgccaccgca gcggcgctga tcccagtctg ctgatcattc
    34621 ggtgggcgat gaccgcccgc cgcgcggaca gccgaatcat cccttcactc aagcgggtga
    34681 acgaggatcg attttcggcc aagcacccgg tttgcgttcc gagcggcttc gcgccgaccc
    34741 cgccgtagac tcatgccgcg gcccccatgg cccaaccggc agaggcaagc ggcttaaacc
    34801 cgctcaagta tgggttcgaa tcccattggg ggcacg
//
LOCUS       DAOPRQ010000022        34671 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_971_length_34671_cov_13.309539, whole genome shotgun sequence.
ACCESSION   DAOPRQ010000022 DAOPRQ010000000
VERSION     DAOPRQ010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 34671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34671
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_971_length_34671_cov_13.309539"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(65..1057)
                     /locus_tag="PKJ61_04395"
     CDS             complement(65..1057)
                     /locus_tag="PKJ61_04395"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF008277.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HOC13096.1"
                     /translation="MTDDALILLANDSYRIEIMPLGATLRRFEVRLPDGSWRNILLGH
                     PDPADYPSGLGYLGATVGRFANRIARGRFSLDGVDYQVDVNEAPNQLHGGTKGFHTHA
                     WSVLGSGPDWVELGITSPDGDQGYPGEVQASARFELIPGGAQVTYRATTDAPTVVNLT
                     THPYFNLDGEASGSTDDHRLTIHASRYTPVYPDLIPTGEVRDVTGSAADFRSGPLLGK
                     AREAAMVEGIALGGGFDHNFVVDGEGMREHCRLAGSTGLTLTVVSDQPAIQVYGGAHF
                     DGVTPGTSGAPYVSRAGVALETQHYPDTPNHPSFPSTVLRPGEEYVATTRWLVG"
     gene            complement(1443..2717)
                     /locus_tag="PKJ61_04400"
     CDS             complement(1443..2717)
                     /locus_tag="PKJ61_04400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HOC13097.1"
                     /translation="MTSTPTTRVGSIDLGPQWLFLVILAGLGMVGPFSIDTIFPAFAQ
                     MEREWGVTELALQQLISVYLLSFAVMSLLHGPLSDALGRKPVIIGGTLLFIAASIGCA
                     LSPSLGVLLFFRSLQGISAGAGQIISRAMVRDVFDNDQAQRTMSHIAMIFGLAPALAP
                     IVGGWVLGAGSWRGIFWFLTAFGVVLLALVIFALPETHPPEQRSKFDVPVLASGLLAV
                     WRDSNGRRLAFTGMFNFAGMFLYISSAPLLVVKLLGKGEQDFWMLFVPLISGMVIGSW
                     VSGRLAGRMSGRRLASLGYVISLVAAVVNLGLALVPATQVLPWPVVALPFYSFGIALA
                     FPILTLAMLDLFPRSRGAASSVQSFVSLVANALIAGALAPAVGFSLWSLAVTSLLLTL
                     VAWWLWRRHLAVTHVEPHTTVDAPAYEPTDEM"
     gene            2795..4009
                     /locus_tag="PKJ61_04405"
     CDS             2795..4009
                     /locus_tag="PKJ61_04405"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOC13098.1"
                     /translation="MPRALNLAYVSMHTSPAQLPGSGDAGGMNVVERAQAEELAELGH
                     QVELITRRTSPDEPDVVQLAPGVVLRHLDAGPPTPLPKSEIDAHIAEFSDGLAKLEPA
                     DLVHSQHWMSGVAALPVARAWGVPHVQSYHSVAALPGSPLSSGEPPESSARVAGEAMV
                     ARESQAVVAISAAEADTVIFRCGADADKVTIISPGVDQELFRPLAPGEALWTWPDAGP
                     WPLGYVLFAARLQPLKGPDLAIGALAHLDPAIRPHLVIAGDTSADFASYENELRRLVT
                     DLGLADDVTFVGPQPRPELARLMRSARVVLVPSHSETFGLVALEAEASGTPVIAAAAG
                     GLREAVAHGETGQLMDSRLPEDWGKALTKLLTQPGLAEQMGVVARVHARRFEWRWAAQ
                     RLVAVYEDLLQR"
     gene            4006..4737
                     /locus_tag="PKJ61_04410"
     CDS             4006..4737
                     /locus_tag="PKJ61_04410"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOC13099.1"
                     /translation="MTPDFTRVVFLHAHPDDETLVTGALLAALAASGREVAVVTATRG
                     EQGEIVPGTFTGSGPALVAHREAELASALAALGVTRHAFLGAPPALAIGALARRYTDS
                     GMRWVTPTVAGPSATAGPDSLTAADPDAAAADLVAYLEHVGATALVSYDAHGGYGHPD
                     HVSCHEIAVRAAERSGVPLFEIVSEPLLPDAAAVEFANPERLPAVKRALAHHASQLTV
                     AGEELVHVGGQRQPIMTSVWLRPRS"
     gene            5038..6201
                     /locus_tag="PKJ61_04415"
     CDS             5038..6201
                     /locus_tag="PKJ61_04415"
                     /inference="COORDINATES: protein motif:HMM:NF022702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HOC13100.1"
                     /translation="MSRRTAMIGAIGVAGAAVVGCSTQPPTGSTSAPAPNPGAPEATS
                     ATPSPTPTEDTTPRWPLTGKVLDDPEDAKRVVVAVKVPDNKGEHPQIGLDKADIVYVQ
                     LDGYPAAVGQSGTRLVPVYHSKYAETVNPVRSIRPVDIPMLSPMTGIIGSTGAYPWVL
                     RYAAEFEDYLVTGKQYMTTKGTGSYSILSSRVRTLNGQTYYDRAVACHPATLAKQSKK
                     FSEGPQQHYLPWAATDEEVSTEVAGQPASFVSVPWKKGDTYNMSYSYSKKKKRYLRSM
                     PWGKHVLADGTQVSCDNVLVICAKQVFGKIYSSGKIKTSGPYHMEPIHQIINTKGTFY
                     YAHGGKFVTGTWTKGEVSEVFQFTLEDGTPLKMAPGQTYIELPNKGAKVTIKE"
     gene            6242..8083
                     /locus_tag="PKJ61_04420"
     CDS             6242..8083
                     /locus_tag="PKJ61_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016668767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOC13101.1"
                     /translation="MRSMVRILGVARELLPAYAAIVLAGLLTAATGLAVPFIVKAATD
                     EVVAATTGAGGGGVPAVLWLAFLLLLTDLANTLITNFGGYLGDVMAIRLRSILSRNYF
                     EKLLRLPQRYFDSELTGTIISRLQRSITETTGFLNMFANNFLPMLLTVAASLVIIAWY
                     SWWLALLMIAVYPVFTWLTMLTSRRWQRLETAKNAEFDIAGGRFAEVVGQIKVVKSFG
                     AEARELSQFSGRYDSTVALTREQSTYWHRMDVARRGALNVIFFGIYAIIFVATISGSF
                     TLGVMVLLIQLVSLARQPVQSMSYLVDASQRAITGSRDYFKVMDEPTETASIELEPTV
                     IPATRVRPASGVIPAEAGIPTATPVVEFRDAHFGYTEAAQVLAGVSFGVFRGERVAFV
                     GESGVGKTTIASLLLGLYPLTSGSLLVNGREVAAIPLAELRSQVGVVFQDPSLFSGTI
                     RENLAYGRPSATEDEIVTAAKRAHAHVFISALPGGYDAEIGERGVKLSGGQKQRIAVA
                     RAMLKDAPILVLDEATSSLDSKAERLVQDGLEELMTDRTTLIIAHRLSTISSVDRIIT
                     LREGQIDEIGTPAELARTGGIYAELLALQASSTKAARKLLQRYEILG"
     gene            8162..9337
                     /locus_tag="PKJ61_04425"
     CDS             8162..9337
                     /locus_tag="PKJ61_04425"
                     /inference="COORDINATES: protein motif:HMM:NF018613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOC13102.1"
                     /translation="MSEATGAADAKKRILFLFSDTGGGHRSATEAMIEALDLEFPGRF
                     DSHKVDFFREYYPSPLRFAPEIYPPFSRIPNAWGLSFKASDGKSRARALNGMTYPYLR
                     RSAKRLVNQNPADLIVSVHPLVNTTLMRAMRNSPRPYITVVTDLVSTHAFWFDRRADL
                     VVVATEEAKAKAIEYGIPEGNLRVIGLPVADRFTHPEFDRTAWRQQMGWRTDLPVILL
                     VGGGDGMGPLKRVAKAINDANLPASLVVICGRNAELKAELEAVEWRMPTHIYGFTTEM
                     PKFMTASDMLVTKAGPGTISEAFIARLPLILYARLPGQEDGNVTYVENERAGVWAPRP
                     REVVDAVRHWVENPAAREAAAIASARVARPNAARDIARLIADQVGVRPTTVRVESQA"
     gene            9670..10419
                     /locus_tag="PKJ61_04430"
     CDS             9670..10419
                     /locus_tag="PKJ61_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13103.1"
                     /translation="MVSQQNEPPEKEEKEPIKVSFSLPQLIGGALAAATAAAVGSSLG
                     VAGTIFGAAVASVVGGVGGTLYSAGIDRTHRKVASVITRTGPVPVEPADGVDLTLLDV
                     TAGAPPVAGSASGSASRSAGGDDPSGRKKRIWKVAAITAAAIFAISLVVITVVELGLG
                     RAFGGGEGTTVSQVVRPKANPSKSVSPTPKPTPSESASSSAPEPSPSESVTPAPSEPT
                     ESSVVPTPAATPTVTVTQVASTEAAAVEPTG"
     gene            complement(10481..10867)
                     /locus_tag="PKJ61_04435"
     CDS             complement(10481..10867)
                     /locus_tag="PKJ61_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020574117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="globin"
                     /protein_id="HOC13104.1"
                     /translation="MPAEASLYDRVGGHETFVKLIDEFYRGVAADEPLRALYPEADLG
                     PAKQRLLLFLEQYWGGPHTYSQTRGHPRLRLRHAPFQVTPDMRDRWLRHMNAALDLLE
                     LPQAELAEFREYVTRAAQFMINSFDN"
     gene            complement(11070..11936)
                     /locus_tag="PKJ61_04440"
     CDS             complement(11070..11936)
                     /locus_tag="PKJ61_04440"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOC13105.1"
                     /translation="MHQLRPLRSASVVLACAVALTGCTTPTPDAPKVTSASAPSLRSA
                     VLGAAVPDTSPAVDLRAHSATEAMPAGTLAGRLIVIDPGHNGVWTKKLGKLVPSGNGR
                     KKACNSSGTATNSGYAEHAYNWAQANTLAEELRARGAEVLLTRSDDSSQGPCVNLRST
                     LANARGADVLISLHADGSYSKKARGFHVIISTTMVGGAALEAESKALAKDLRSALETG
                     TAMPRSTYIGKGTALSPRSDIATLNLSKRVAVMMEMGNMRHPKDAALLKSAKFRTQAA
                     VALADGIASYLG"
     gene            12172..12453
                     /locus_tag="PKJ61_04445"
     CDS             12172..12453
                     /locus_tag="PKJ61_04445"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007117347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOC13106.1"
                     /translation="MTEEFSHNQAESRFEVHIDGELAGECHYRLNGDVAVFDHTVVPP
                     EFGGRGIAGRLVTHAMDEVRTAGAWKVRPACSYVAGWFGKHPEYADLLA"
     gene            12553..14907
                     /locus_tag="PKJ61_04450"
     CDS             12553..14907
                     /locus_tag="PKJ61_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13107.1"
                     /translation="MSSQPIIIDAEQTRQRSEACLASGDFKAADAGYAELRRLGALSA
                     SDAGKWLRAVRALTQLGELDENDQRPSLEELVAAFPDDQDLLEFVAAEVWQRAESHAV
                     PGEEDAEIARRAWQAALHMAGPVPESDAAGVRLIRLVDRFFQAGRRQQTPIGSYPEYE
                     QALVRFAAAGPESTLRAADLLAAESPVLALELVATSEISAASVLVRAVASAEMEDHVG
                     ATSLFDELTARWGQGFETLDVEDRSRWVQSMTDVGRFAEAEAACLRTIEQIEHSPETL
                     AGMDGAQDHWRHVYHLLAYRLAAQQGRYREAWDHLRRASTVLDADLSPELRRSVLRVR
                     ILLADRDEAEGILAELAELASCDPYDTAYAEACLWAGTQWGTPDADASHHASGGTFSG
                     ARYAGQAAARALLAQAGPEVSSRQLIRLALLAGDDDRAQALLTAEPLVASDPWPAKVL
                     AALVALRQGDDLTANRLIAEVMPQRRHDLDLRVLDAQASLISGDYKVSLREAMAITDS
                     VGVHILARVIRAESEFEAALAMVADEDASHGSVENAQQLMAAVSDYRTVADLQRDTIG
                     YLRTAAAPDAVAIGSEPLPPRLFTEVCRRGLHAAILAQEALDRLGLRGDRQLVTDAQD
                     LVQHLRSVTRPCCRTWGSRPSRLLHQVRHLVDRDEATRLALLMISHRWSAWRRRLQNF
                     GFIGLGALVTWAGLTNVLPGPESDTIRALVLGLGVLLLLMPFARSLKVGVVELSRDAD
                     AAPLSGRSKSLRTSRLLLRANHLGTFPLPSPPEKGRRAHAQSRD"
     gene            complement(15083..15559)
                     /locus_tag="PKJ61_04455"
     CDS             complement(15083..15559)
                     /locus_tag="PKJ61_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002544326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOC13108.1"
                     /translation="MPTPDFIIELRNHVGSMPLWLSGATGVVLREQADASEVLLVRRS
                     DNGAWTPVAGIVDPGEHPHHTAIREIAEETGVVAEIDRLVWLTVTEVITYENGDQSQY
                     IDHVFRCRWTSGEPYPADGEASAAAFFPLDQLPRMSARHRRQVEVAVANAPETLLG"
     gene            15946..16443
                     /locus_tag="PKJ61_04460"
     CDS             15946..16443
                     /locus_tag="PKJ61_04460"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020579218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOC13109.1"
                     /translation="MFRTEASPGPAVLVRPMRTGDAAGVLAVQLAAFTIESELYDDPE
                     LPPLLEPHADLNHWLDAGGLGFVAEADGRLVGSIRIELDGDVVLISRLSVAPEYQRRG
                     IGEALLRHAESVTPAVTARLFTGHLSAANLRLYARLGYTEERRQPVDAKVTLVHLRKD
                     LRPSD"
     gene            complement(16517..18271)
                     /locus_tag="PKJ61_04465"
     CDS             complement(16517..18271)
                     /locus_tag="PKJ61_04465"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOC13110.1"
                     /translation="MPIIVSGLVSSLLSVGPAGAAPAAAIAEQVKTGLAANVGAATTS
                     AKEAKRVDRVRVSKLKWHKASDGSGYSATVKLPLDYDKPKGAKVRVALFKVPAADPTN
                     RVGTLFLNPGGPGGSGVALARTASTFLSQEILNKFDIVGFDPRGTNGSTQVRCFSSAA
                     KQTSALSGMSVAFPVDAAQEELYLKSAKKLGKACSKRARKLASAMSTAEVARDLDVLR
                     RTLGGKSAKLSYLGFSYGTYLGEVYASLFPDRFRAMALDGVVDPTAWAGNSSTAGSPT
                     TIRIRAAEGAWQALQTGLERCAAKGPQYCPLADPKADFDRVAAELRIRGWVDVADDGG
                     AFRFTYTEYISAVLSLLYYPSGMDYVAELTARLDDVLYPGSSATLRRAGKNLMTLVKS
                     YQRAARTQSVLIGTYDNELDAYSSVICTDSREPRWPGTWQLAATRADAAVPHFGRLWA
                     WSDVQCATQYWKAKDEDVFRGSFIQPTAAPVLLVGNYQDPATNYDNAVQTARLMPNSY
                     LLSSDSWGHTAYGTSACVTSRVDAYLLTGAQPDGTVCIGDEQPFSSPLESVSDTASAL
                     AKDLAREGLPPVEPPVAG"
     gene            complement(18423..18920)
                     /locus_tag="PKJ61_04470"
     CDS             complement(18423..18920)
                     /locus_tag="PKJ61_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin/nitric oxide synthase"
                     /protein_id="HOC13111.1"
                     /translation="MSALIVTESIFGNTLAIAQAISGGLADVLGAREVQLMHASDAPD
                     SLPAEVALLVIGAPTHTLSMPNAGSRADAAGQGATVAERTGVREWIEVLELPKGIRVA
                     TFDTSIQERQRLGTAARAAAIALRDRGAETLIGPSFYVTGMEGPLADGELGRATAWGR
                     ELATA"
     gene            complement(19251..19430)
                     /locus_tag="PKJ61_04475"
     CDS             complement(19251..19430)
                     /locus_tag="PKJ61_04475"
                     /inference="COORDINATES: protein motif:HMM:NF019057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HOC13112.1"
                     /translation="MGKFEIYPDAAGKYRWRLKASNGQIIASGQGYATERSCRDGIES
                     VKKHAASAEVVEVEG"
     gene            complement(19514..21202)
                     /locus_tag="PKJ61_04480"
     CDS             complement(19514..21202)
                     /locus_tag="PKJ61_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012918930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HOC13113.1"
                     /translation="MSTQASTTRPTADENWWRSAVVYQIYPRSFADSNGDGVGDINGI
                     RAKLGHLSALGVDAIWINPWYPSPMKDAGYDVSDYRAIEPVFGTMADAESLIAEAHAA
                     GIRVILDIVPNHTSDQHRWFTEALEAGPGSPERERFIFREGRGPAGDEPPNDWMSEFG
                     GPAWTRVTEADGLPGQWYLRLFAPEQPDVNWDHPDIQAEFEETLRFWFDRGVDGFRID
                     VAHGLVKDAGLADVNGLPFPLPADTPEDLDHPHWDRPGVHDIFREWRRVADSYDPPRV
                     FCGEIWVGRTHRLTAYLRPDELHTAFNFEFLTSPFRAEAMRVTIEATLASHAGVGAPT
                     TWVLGNHDICRPVSRYARPQELVGPTDRNLSHFIDLPADFEAGTRRARAAALLSLALP
                     GGAYIYQGEELGLPEVEDIPLAAMQDPMVAGNNYENLGRDGCRVPLPWTASGSNYGFS
                     PDGSAAPWLPQPSGWSVLSAEIEALDEASTLNLYRRALALRAEHPALGDGTLTWVEAG
                     ADALAFTREPGFGCWVNFGAKPLALPDGASVLLASAELVAGLLPPDAAAWLQLA"
     gene            complement(21330..21899)
                     /locus_tag="PKJ61_04485"
     CDS             complement(21330..21899)
                     /locus_tag="PKJ61_04485"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HOC13114.1"
                     /translation="MHPLPRALPFRLVAILAGAFITLPVTQNAASATAAAVPAVVTSG
                     YTVKYEVSSMDNEVLRLVNEARSAKRKCGKTTYKKVKPLVWSDSLGQAAVAHSTDMAS
                     GNYFSHYSRNGASPFTRIKATGYRYSSAGENIAAGYDTPAQVVKAWLKSPGHCKNIMK
                     KSYTQLGVGYAEGGKYGTYWTQDFGKPKK"
     gene            22161..23069
                     /locus_tag="PKJ61_04490"
     CDS             22161..23069
                     /locus_tag="PKJ61_04490"
                     /EC_number="3.1.3.16"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase"
                     /protein_id="HOC13115.1"
                     /translation="MSERLAVGGDLSANFRRARSQLDELVAAGITHIVDLRSEWTDET
                     LVAEWAPQVRYLHHPVADAGQVIGPDWFAELVGWVDDALDDPQAKVLLHCHMGVNRAP
                     SAALAVLLSQGVELRPALNAIRGARPVAIIDYARHALDWHLLTSGADARARRNARRRL
                     GRWRGSNQLDATQVIRAIRDTETAANRWWLRLDEATPDLLAELLESAGELAIGLPVTA
                     EPPELSLFDEVFLITVQGLVGRALVVGPPRAGESDEKLLPLLARELFPPVALTLPRRV
                     ANWLRGARPNLRRIPDAEYRALTLAN"
     gene            complement(23112..24488)
                     /locus_tag="PKJ61_04495"
     CDS             complement(23112..24488)
                     /locus_tag="PKJ61_04495"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016668883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOC13116.1"
                     /translation="MAVKLTFGGGAGTVTGSKYLVNSDDRRILVDAGVFQGEKRLREL
                     NWTPFPTAPESIDTVLITHAHTDHVGYLPVLVRNGFHGEVLATQATCELAEIVLRDAA
                     FLAERDAEHAASHSYSRHQPPLPLFTAADAERALDRMRVIDFDTEVSLGHGLAAAWTR
                     AGHILGSASIRLGTPTGSVLFSGDLGRATHPVLRPREIPAGADVVLIESTYGDREHHE
                     PSGPEHEPLADLIRRTAKRGGSVLIPAFAVDRTEVVLQTLTDLLHAGRIPELPVFVNS
                     PMALAALRVYTSATHADELRPDLDPRSFVDLPGLREVRSTEESIKLNDPKVPCIIISS
                     SGMATGGRVLHHLEHMLPDDRHSVVLVGYQAEGTRGRLLADGVRSLKMHGRNVAVRAE
                     ILQDEEFSVHADRSELLDWLGALEPRPQQVYTVHGTEDSASAFAREITVRLSIPARPA
                     ELLETVEL"
     gene            24754..28128
                     /locus_tag="PKJ61_04500"
     CDS             24754..28128
                     /locus_tag="PKJ61_04500"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOC13117.1"
                     /translation="MLAVPAVAEAAPAGTDDTLTTARSSGRVSTTLTPRVLAGGALVD
                     VMVQLEADPVAVAEAKAERTFTAAERTTHKNKLRKAQDAITDDIAGKGGKVISRMQSA
                     YNGMRVRINRSRADALAALPGVSGVHAITAKTLDTSVSVPYLGVPSVWQDTGFTGKGV
                     KVAIIDSGIDYTHAVFGGPGTVAAFEAAAATATQAANPEWFGPDAPRVKGGWDLVGDA
                     YDSDAEPGSPAATPVPDPNPLDCNGHGTHVAGIAGGSGVTASGDVYTGAYNAKTAKTQ
                     FSIGPGVAPQVDLYAVRAFGCEGSSEVVAEAIDWAVERGMDVINLSMGSTFGRADDPD
                     AVAASNAVGAGVVVVTSAGNEGPNPYLAGSPGVGEGVISVAAVDSTPSFPGASLSFAG
                     QQLTAINANAADLPGTALTVVNVTDDPDTAEVESLGCSVAAFTQAGIVPDGHQIAVVE
                     RDGCSRPAKAIFGQQAGAAAVVMINTIADGYPPLEGEITYNPDDGMPYQVTIPFLGVP
                     NTAAAALAGAEGAAMTMTATTVANPALGEIAAFSSAGPVNGSSEVSPSLAAPGVSITS
                     AGIGTGDGTTAMSGTSQAAPHVTGVAALGVQAHPKWSAQDIAATLVNTADPAKVSGYS
                     LTLAGSGLVDAAQTVTTESLVTGDRYRTSSGVMAEPSLSFGFAEPSLTFLGTKWITIH
                     NRGCEAITYKLGAEASAASRPASLKFGAKKVKVPARSKVMVPVTLLVNAAKIGSSLTD
                     TQAGFREVSGNIVLTTGGDELRVPYLLVPRARAQVSAKALDRFPKPVAAPEYLAADAA
                     DAADESAAEVEANSPAESVNVVEPVEDLGADPDAEVAAGEPESSAGADGVAEAAQQVT
                     DDPIEEPVAEAVEAVEEIAPTAPTAPNSTAQTTTVRVSLRNPLGAVDASADFYTWGLT
                     DPKDPSARGGYDLRAAGVQSYANGADTLLLFAVNNHSRWSTAATQEFDVSVDTNGDGA
                     ADYVVLSYDSGYIATGTYNGLTGVFVYDVAAGAMSASGYNAVAPTDSSTILLPVNASA
                     LGLSADAGDFSYTVDSYSILDPVAGDSFGAWANYNPWAKAIGDSAYVTVRRNRTLKVP
                     VEVDPVALGTQRPLGVMVVAFDNQSGASEALLLKAR"
     gene            complement(28183..28572)
                     /locus_tag="PKJ61_04505"
     CDS             complement(28183..28572)
                     /locus_tag="PKJ61_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13118.1"
                     /translation="MVRAALVSTMAVLLCGCASRGEMGERPPLQTMSPLPSVTPSAGT
                     VATPSPQQWDAILADLAARQVPTTDVRVVTSRRVTWNDGSLGCPKPGQLYTQAQVPGM
                     QVVVSVAGVEYDYRFGRSGEPRLCRPA"
     gene            complement(28703..30247)
                     /locus_tag="PKJ61_04510"
     CDS             complement(28703..30247)
                     /locus_tag="PKJ61_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13119.1"
                     /translation="MPTVPANEDSRRELASTVKRLRVAVTSDPSRTAELADAMVALNA
                     TRLQAWAFSEAAADAPDAVVQAARILAQGGASGPYTSLPDGVRFFTASAQLAAVQGFL
                     GLAEAAARTLDGLDAWRNQLGRLPLVDHLSAAAVVWSLLARARAYVDDPALANAYADA
                     ASAWRFVTPMPAAQTIAVHLTAADCRWAGGHPDQALAQHRLALTAHESLLAELPARPR
                     PALVATAAATAAATYEPYASRLESAGQVDFALGVRRAHLALLDRLGAEHAEGQRWLAA
                     ALRRAGRAAEADEFASADGVATAPPTPGRPVTWTRLDASAELSNRDLTSGYAASERAA
                     QESTQAAATARRDAEAAETRHRDQAAERARQLAEDELAAATAAARAAQQEAAARQERE
                     RTEAAAAAERRAKAERAARQAAEQHRQQAELQARLAARAAAEVQDELTLARSAANAAA
                     ESPADRAHNLDRLAGLLRPLTALEAYRDELATTLEALVGLRWQLGEADASREAARELK
                     ALTGSR"
     gene            30344..31006
                     /locus_tag="PKJ61_04515"
     CDS             30344..31006
                     /locus_tag="PKJ61_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007077860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOC13120.1"
                     /translation="MSELIEQPRLTIRRISVSSMDNNVYLLTTRTGGRQLLIDAAHEP
                     DAIAELLASAAGDADVPPRLELILTTHSHHDHLGALAAVASAHPEAQTLAGAADAGAI
                     TAATGVPIARRLEHGDAIALGDLELQVIGLRGHTPGSVAVAYSEPDQQTHVFTGDSLF
                     PGGVGNTNRDAERFNSLFSDVVQRIFEVYRGDSIVHPGHGAATTLSSERPHLAEWEAR
                     GW"
     gene            complement(31036..32385)
                     /locus_tag="PKJ61_04520"
     CDS             complement(31036..32385)
                     /locus_tag="PKJ61_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HOC13121.1"
                     /translation="MADTREPTFDEHFHEDALEPKHLVRRANVVLRAGILMLGAGTSG
                     LRVREVMQAVARTVGIGNIRAQVTFTDIVLTVQRRGTFRTQVGEVRKPGVNAHRIDLM
                     QRLGQNLPERATVAEVDAMLDDIEHTPRLYPAWALILLVALACGSVTILGNGGWREVL
                     AVLPASALAYWLHRTLTGLQLNHLAVVLTSAATATGLYAVFTRGLDLALGSPSPRMAA
                     GLICAAIFLIPGFPLVTAGLDLTRIDLLAGVPRLFYAAMVLLSITIGVWLVVTPTGVS
                     PDAVPPLAIQPALLWPVQLAASFFAVFGWATMFNSPLSAAVMSGVVALLGNIPRLLLL
                     QNGVKAHIATFVCCFVMGLLCALAARLFDLNKIIMTVPTVLVAIPGASALRALIYFDQ
                     ADVVSALQQGVATVLAVIAMVAGLSGARMLTDPEWAFTTDDPPGTAWHLVQRLFRRS"
     gene            32504..32647
                     /locus_tag="PKJ61_04525"
     CDS             32504..32647
                     /locus_tag="PKJ61_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13122.1"
                     /translation="MLLGPGLNLKRHPAGGRNFEYFSEDPLLSGKAAAALVRGIQSEG
                     GSR"
     gene            complement(32775..33653)
                     /locus_tag="PKJ61_04530"
     CDS             complement(32775..33653)
                     /locus_tag="PKJ61_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020578808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter Tol"
                     /protein_id="HOC13123.1"
                     /translation="MAGRELAAGQVCEVHTYDLRTATDSVVYVDTERLFEAPNWTPDD
                     RLILNGDGLLWTLPADGSAPPTEIPLVGAPELNNDHVLAPDGENIYLSGNDRHLYVAP
                     LAGGETRRVTRDRGSLMHFLHGVSPDGTTLAYVNIDPTQGWGCVNLHTVGVDGSDDRE
                     VLPGWGPDDGPEYSVDGEWLYFNTERFSTTPGHAQIARVRPDGTEFTQLTFDERVNWF
                     PHTAPGGRHWVYLSFPAGTLGHPADLTVQLRLVVRDAWADAVTVAELPGGQGTINVNS
                     WSPDGTRFAYVAYPFR"
     gene            complement(33890..34189)
                     /locus_tag="PKJ61_04535"
     CDS             complement(33890..34189)
                     /locus_tag="PKJ61_04535"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOC13124.1"
                     /translation="MMKLLPLSGSGEASADTEVGWHLHSEAWGNGYAAEAGQRLLDRA
                     WKHGLTTVYAVTYPENLASQAVCRRLGMTHLGRTDRYNDVACELFRADRQSPRLG"
     gene            complement(34211..>34671)
                     /locus_tag="PKJ61_04540"
     CDS             complement(34211..>34671)
                     /locus_tag="PKJ61_04540"
                     /inference="COORDINATES: protein motif:HMM:NF019884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HOC13125.1"
                     /translation="TSPTARALRALELLQNQPGITAAELADALAVTERAARRYVAILR
                     QAEIPIRAVPGPGGGYTVGRGIRLPPLMFSATEALGVVMAVLESHDASADDPVGTALR
                     KIMRARWEVVRFLGRTPAVLADRSQAEERVAAWTAFDGALHGVWAIVRSG"
BASE COUNT         5500 a        11689 c        12032 g         5450 t
ORIGIN      
        1 ccagacccca cggcgtgtcg tcggcgtgtc gcgttaccac cgagacaaaa cggacaaggg
       61 gcggtcagcc gaccagccac cgggtggtgg ccacgtactc ctcgccggga cgcagcacag
      121 tcgaagggaa gctcgggtgg ttgggcgtat ccgggtagtg ctgggtctcc agcgccacgc
      181 cggcccggct gacgtagggc gcaccggaag tacccggggt gacgccgtcg aagtgggcgc
      241 cgccgtacac ctggatggcg ggttgatcgc tcacgacggt cagggtgagt ccggtcgaac
      301 cggctaaccg gcagtgctca cgcatgccct cgccgtccac cacgaagttg tggtcgaagc
      361 cgccgcctag ggcgatgccc tcgaccatcg cggcctcccg cgccttaccg agcagtggac
      421 cggaccggaa gtcagcggcc gacccggtca cgtcgcggac ctcgccggtt ggaatcaggt
      481 ccgggtacac cggcgtgtag cgggacgcgt ggatggtgag ccggtggtca tcggtgctgc
      541 cggacgcctc cccgtccagg ttgaagtacg ggtgcgtggt caggttcacc acggtcgggg
      601 cgtcggtggt ggcccggtag gtgacctggg ctccccccgg gatcagctcg aagcgtgcgg
      661 acgcctgcac ctcgcccggg tagccctgat cgccatcagg gctggtgatg cccagttcga
      721 cccaatctgg ccccgagccg agcacggacc aggcgtgggt gtggaagcct ttcgttccgc
      781 cgtgcagctg gttgggcgcc tcgttgacat ccacctggta gtccacgccg tcgaggctga
      841 accggccgcg tgcgatccgg ttcgcgaacc gtccgacggt cgcacccagg tagcccagcc
      901 cggaagggta gtccgccgga tccgggtggc cgagcaggat gttgcgccag ctgccgtccg
      961 gcaagcgcac ctcgaagcgt cgcagggtcg caccgagcgg catgatctcg atccggtagg
     1021 agtcgttcgc cagcaggatg agcgcgtcgt cagtcacggt gataacgcta tcaacgttcg
     1081 gctggatcgc gccttgcgac caaggtggcc ctggtaacgg ccgagcgcag ggaaaccact
     1141 tctggctggt tatgaggccg ctttcgctgg ccgatttcgg ttcaacccga gaaccgcagg
     1201 ttatggagct cgaaagcggc cgcataacct gcatttcttg ggttgggcgg gcgagggtcc
     1261 tctggggagg ggtcataacc agccagaagt gggttgagcc agacggcggc ccttcgttac
     1321 ggccggctcg ttccgcaccg gcctcgtcag ggaacggttt gtgaggggct cgttcctcag
     1381 cgaccgattg tgagcggctc gttccgcact gggtctcgac cggctcggcc agccgtcggc
     1441 ggctacatct cgtcggtggg ttcgtaggcc ggggcgtcga ctgtggtgtg cggctctacg
     1501 tgggtgactg ctagatggcg gcgccacagc caccaggcga ccagggtgag cagcagtgaa
     1561 gtcaccgcca ggctccacag cgagaagccg accgccgggg cgagcgctcc ggcgatcagg
     1621 gcgttcgcca ccagcgagac gaacgactgc accgaggacg ccgccccgcg ggaccgcggg
     1681 aacaggtcga gcatggccag cgtcaggatg gggaacgcca gcgcgatccc gaatgagtag
     1741 aacggcagcg ccaccaccgg ccacggcagc acctgggtag ccggcaccaa cgccaggcca
     1801 aggtttacga ctgccgcgac caggctgatc acataaccaa gggacgcgag ccgccgtccg
     1861 ctcatccgtc cggccagccg tccgctgacc caggaaccga tcaccatgcc ggagatcagc
     1921 ggcacgaaca gcatccagaa gtcctgctca cccttgccca gcagcttcac caccagcagc
     1981 ggggccgagg agatgtagag gaacatgccg gcgaagttga acatgccggt gaaggcgagt
     2041 cggcgaccgt tcgagtcccg ccacaccgcc agcaacccgg acgcgagcac gggcacgtcg
     2101 aacttcgacc gctgctccgg cgggtgcgtc tccggtaacg cgaagatcac cagcgcgagc
     2161 agcaccacgc cgaacgcggt caggaaccag aagatcccgc gccaactgcc tgcgcccagc
     2221 acccagccgc cgacgatcgg cgccagcgcc ggagcgaggc cgaagatcat cgcgatgtgg
     2281 ctcatcgtcc gctgggcctg gtcgttgtcg aagacgtcac gcaccatcgc gcggctgatg
     2341 atctggccgg cgccggcgct gatgccctgc agcgatcgga agaaaagcag caccccgagg
     2401 ctcggcgaca gcgcacaacc gatggacgca gcgatgaaca gcagcgtgcc gccgatgatc
     2461 accggcttgc ggcccagtgc gtccgacaat gggccgtgca gcaggctcat caccgcgaag
     2521 gagagcaggt agacgctgat cagctgttgc agtgcgagct cggtgacccc ccactcgcgc
     2581 tccatctggg caaaggcggg aaagatggtg tcgatcgaga acggccccac catgcccagc
     2641 ccggcgagga tcaccaggaa caaccactgc gggccgagat cgatgctgcc gacgcgagtg
     2701 gtcggggtgg aagtcattgc gtgattcttc cacgggctga gagcgctctg cgctcgcgtc
     2761 cggtcgctgt gcatcgggtg actatgctca gcgcgtgcca cgcgctctaa atctcgccta
     2821 tgtctccatg cacacctcgc cggcgcagct gccgggttcg ggggacgccg ggggcatgaa
     2881 cgtggtcgag cgggcgcagg ccgaagagct cgccgaactc ggccaccagg tggaactcat
     2941 cacgcggcgc accagtcccg acgagccgga cgttgtccag ctcgcccctg gcgtggtcct
     3001 gcgccatctg gacgccggcc cgcccacgcc gctgcccaag agcgaaatcg acgcccacat
     3061 cgctgaattc tcggacgggc tggcgaaact cgaaccggcc gacctggtgc actcccagca
     3121 ttggatgtcc ggtgtcgcag cgctgccggt ggcccgcgcc tggggcgttc cacatgtgca
     3181 gagctaccac tcggtcgcgg cgctgcccgg ctcaccactc tcgtccggcg agccgccgga
     3241 atcgtccgcc cgggtggccg gcgaggcgat ggtggcccgg gagtcccagg ccgtggttgc
     3301 gatcagcgcc gccgaggccg acacggtgat cttccggtgt ggcgcggacg cggacaaggt
     3361 cacgatcatc tcgccggggg tcgaccagga gctgtttcgt ccgctggccc cgggggaggc
     3421 gttatggacg tggccggatg cgggcccgtg gccgctcggc tatgtgctct tcgccgctcg
     3481 gctgcagccg ctgaaaggcc cggatctggc gatcggcgcg ctggcgcatc tcgatccggc
     3541 gatccggccg cacctggtga tcgcgggtga cacgtccgct gacttcgcca gttacgagaa
     3601 tgagttgcgg cggctggtga ccgatctcgg gctggccgat gacgtcacct tcgtcgggcc
     3661 gcagccgcgt ccggagttgg cccggctgat gcgctcggcg cgggtggtgc tggtgccgtc
     3721 acattcggag acgttcggcc tggtcgcgct cgaagccgag gccagcggga cgccggtgat
     3781 cgcggccgcg gcgggtggac tgcgcgaggc ggtggcgcac ggcgagaccg gtcagctgat
     3841 ggactccagg cttcccgagg actggggcaa ggcgctgacc aagctgctca cccagccggg
     3901 cctggccgag cagatgggcg tggtggcccg cgtccatgcg cgccggttcg aatggcgctg
     3961 ggcggcgcag cggctggtcg ccgtgtacga ggacctgctg cagcgatgac ccccgacttc
     4021 acccgtgtgg tcttcctgca cgcgcacccg gacgacgaga cgctggtcac cggtgccctg
     4081 ctggccgcgc tggccgcatc cggacgcgag gtggcggtgg tcaccgcgac tcgcggcgag
     4141 cagggtgaga tcgtgccagg gacgttcacc ggcagcgggc cagcgttggt cgcgcaccgg
     4201 gaggctgagc tcgcgtctgc cctggccgcg ctcggggtga cccggcacgc cttccttggc
     4261 gccccacccg ccttggcgat tggcgcgctg gcaaggcgat acaccgattc gggcatgcgc
     4321 tgggtcacgc cgacggtggc cggtccgtcc gccaccgccg gcccggacag cctcaccgct
     4381 gccgacccgg acgccgccgc ggccgatctg gtggcctacc tcgagcacgt cggggcgacc
     4441 gcgctggtca gctacgacgc gcacggcggc tacggacacc ccgaccacgt gtcctgccac
     4501 gagatcgccg tgcgggcggc cgagcgcagt ggggtgccgc tgttcgaaat cgtctcggag
     4561 ccactgctgc cggacgcggc cgcggtcgag ttcgccaacc ccgagcggct acctgcggtg
     4621 aagcgagccc tggcgcacca tgccagccag ctgaccgttg ccggtgagga gttggtgcac
     4681 gtcggtggcc agcgccagcc gatcatgact tcagtctggc tgcgtccgcg aagttgagcc
     4741 tgcggagggg aacgaaccgg atttcgggag gtgatggcgg cgatgctgct gcccgcgcct
     4801 cagcaaaccc gagtcggtag gcttatcggg ctggggtccg gcttgataac ccagccaagt
     4861 cgggtcgcgt ctcctcggga gcagtggcgg ggggcacgca gccaagcctt cgaatcagct
     4921 gtgaaccgac ccagcggacg cgaagcgtgc gggcttgcgg gcacgcgccc cttaccatgg
     4981 acgcggtgag cgtggcagca ggcccgcaca acagcagtcg gaggaagcat gggatcggtg
     5041 agccgtcgca cggccatgat cggcgcgatc ggcgtcgcag gagcagccgt ggtggggtgt
     5101 tcgacccaac ctccgaccgg gtccacctcg gccccagcgc ccaaccccgg cgcgcccgag
     5161 gccacgtccg caacaccgag cccgacgccc accgaggaca ccacgccccg ctggccgctg
     5221 accggcaagg tgctggacga ccccgaggac gccaagcggg tagtcgtggc cgtgaaggtc
     5281 ccggacaaca agggtgagca cccgcagatc ggcctcgaca aggccgacat cgtctacgtc
     5341 cagctcgacg gctacccggc agcggtcggc cagtccggca cccggctggt gccggtctac
     5401 cactccaagt acgccgagac ggtgaacccg gtgcgctcga tccgtccggt ggacatcccg
     5461 atgctctcgc ccatgaccgg catcatcggc agcaccggcg cgtacccgtg ggtgctgcgc
     5521 tatgccgccg agttcgagga ctacctggtc accggcaagc agtacatgac caccaagggc
     5581 accggttcct actcgatcct gagcagccgt gtccgcaccc tcaacggcca gacctactac
     5641 gaccgcgcgg tggcctgcca cccggccacc ctggccaagc agagcaagaa gttcagcgaa
     5701 ggtccgcagc agcactacct gccgtgggcg gcgacggacg aagaggtcag cactgaggtc
     5761 gccggccagc cggccagctt cgtgagcgtc ccctggaaga agggcgacac ctacaacatg
     5821 agctacagct acagcaagaa gaagaagcgc tacctgcgca gcatgccgtg gggaaagcac
     5881 gtgctggcgg acgggacgca ggtctcctgt gacaacgtgc tggtgatctg cgccaagcag
     5941 gtcttcggga agatctactc cagcggcaag atcaagacgt ccgggccgta ccacatggag
     6001 ccgatccacc agatcatcaa caccaagggc accttctact acgcgcacgg cggcaagttc
     6061 gtcaccggca cctggacgaa gggcgaagtc agcgaggtgt tccagttcac ccttgaggac
     6121 ggcactccgc tgaagatggc gccgggccag acctacatcg agttgccgaa caagggcgca
     6181 aaggtcacga tcaaggagtg aggccgaacc ggccactccg actggacgaa gtgaggctgg
     6241 gatgaggtcg atggtgcgga tcctgggcgt tgcccgggag ttgctgcctg cgtacgccgc
     6301 gatcgtgctg gccgggctgc tcaccgctgc gaccggcctg gccgtgccgt tcatcgtcaa
     6361 ggccgcgacg gacgaggtcg tggccgcgac cactggagcc ggcggaggcg gggtgccggc
     6421 ggtgctctgg ctggcgttcc tcctgctgct gactgatctg gcgaacacgc tgatcaccaa
     6481 cttcggcggc tatctcggtg atgtgatggc gatccggctg cgctcgatcc tgtcccggaa
     6541 ctacttcgag aagctgttgc ggctgccgca gcggtacttc gactccgagc tcaccgggac
     6601 gatcatttcg cggctgcagc gctcgatcac cgagaccacc ggcttcctga acatgttcgc
     6661 gaacaacttc ctgccgatgc tgctaaccgt ggccgcctca ctggtgatca tcgcctggta
     6721 ctcgtggtgg ctggcgctgc tgatgatcgc ggtctacccg gtgttcacct ggctgaccat
     6781 gctcacctcc cggcgctggc agcggctgga gaccgcgaag aacgccgagt tcgacatcgc
     6841 cgggggacgc ttcgccgagg tcgtcggcca gatcaaggtg gtgaagagct tcggcgcgga
     6901 ggcccgcgaa ctgtcgcaat tcagcggacg ctacgactcg accgtcgcgc tcaccaggga
     6961 gcagtcgacc tactggcacc gcatggacgt ggcccgtcgc ggcgccctga acgtcatctt
     7021 cttcggcatc tacgccatca tcttcgtggc cacgatctcg ggttcgttca cgctcggcgt
     7081 gatggtgctg ctgatccaac tggtcagcct ggcgcgccag ccggtgcagt cgatgagcta
     7141 cctggtggac gcctcccagc gtgccatcac cggtagtcgc gactacttca aggtgatgga
     7201 cgagcccacc gagaccgcgt ccatcgaact tgagcccact gtcatcccgg caacaagagt
     7261 tcgccccgcc tcaggtgtca tcccggcgga ggccgggatc ccgacggcga cccccgtggt
     7321 cgagttccgc gacgcccact tcggctacac cgaggcagcc caggtgctcg ccggcgtgag
     7381 ctttggggtg ttccgcggcg agcgggtggc gttcgtcggc gagagcggag tgggcaagac
     7441 gacgatcgcg tccctgctgt tggggctgta ccccctgacc agcgggtcgc tgctggtcaa
     7501 cggacgcgag gtggcggcca ttccgctggc cgagttgcgg tcgcaggtcg gagtggtgtt
     7561 ccaggatcca tcgctgttct ccggcacgat ccgggagaac ctcgcctacg gccgcccgtc
     7621 cgcgaccgag gacgagatcg tcaccgcggc caagcgggcg cacgcccatg tgttcatcag
     7681 cgcgctgccc ggcggctacg acgccgagat cggcgagcgc ggggtgaagc tgtccggtgg
     7741 ccagaagcag cggatcgcgg tggccagggc gatgctgaag gacgcaccga tcctggtgct
     7801 ggacgaagcc acgtcctcgc tggattccaa agccgagcgc ctggtgcagg acggactcga
     7861 agagctgatg accgatcgca ccacgctgat catcgcgcac cggttgagca cgatcagctc
     7921 ggtcgaccgg atcatcacgc tgcgcgaagg ccagatcgac gagatcggta cgcctgccga
     7981 actcgcccgt accggcggca tctacgccga actgctcgcg ctgcaggcgt ccagcaccaa
     8041 ggccgcccgc aagctgctgc agcgctacga gatcctcggc taaggccccc ggccgcgcac
     8101 ccagcgggtc gcggctgtga catgctctag tgaggcagga ccagcgggag acgggatggg
     8161 catgagcgaa gcgacaggtg cggcggacgc gaagaagcgc atcctgttcc tgttctccga
     8221 caccggtggc ggccaccggt cggccacgga ggcgatgatc gaggcgctcg acctggagtt
     8281 ccccggccgt ttcgattcgc acaaggtcga cttcttccgc gagtactacc cgagcccgct
     8341 gcggttcgcg ccggagatct atccgccgtt ctcacggatt ccgaacgcct gggggctctc
     8401 cttcaaggca tcggacggga agagccgcgc gcgcgcactg aacggtatga cctatccgta
     8461 tctgcggcgc tcggcgaagc ggctggtcaa tcagaatccg gccgacctga tcgtttcggt
     8521 gcatccgctg gtgaacacga cgttgatgcg ggccatgcgg aactcgccgc ggccctacat
     8581 cacggtggtc accgacctgg tctcgacgca cgcgttctgg ttcgaccggc gcgctgacct
     8641 ggtcgtggtg gccaccgagg aagcgaaggc gaaagcgatt gagtacggca tccccgaggg
     8701 caacctgcgg gtgatcgggt tgccggttgc cgaccggttc acccatcccg agttcgatcg
     8761 gacggcctgg cgccagcaga tgggctggcg gacggacctg ccggtgatcc tgctggtcgg
     8821 tggcggcgac ggcatgggtc cgctgaagcg ggtcgcgaag gcgatcaacg acgcgaacct
     8881 gcccgcgtcc ctggtggtga tctgcgggcg caatgccgaa ctgaaggccg aactcgaagc
     8941 tgtggagtgg cgcatgccca cccacatcta cgggttcacc accgagatgc cgaagttcat
     9001 gaccgcgtcc gacatgttgg tgaccaaagc cggcccgggc acgatcagcg aagcgttcat
     9061 cgcgcggttg ccgctgatcc tgtacgcccg gctgcccggg caggaggacg ggaacgtcac
     9121 ctacgttgag aacgagcggg ccggggtgtg ggcgccgcgt ccgcgcgagg tggtggacgc
     9181 ggtccggcat tgggtggaga acccggccgc ccgcgaggct gcggcgatcg cttcggcccg
     9241 ggtggcccgt ccgaatgccg ctcgggacat cgcccgcctg atcgccgacc aggtcggagt
     9301 gcggccgacc accgtccgtg ttgagagcca ggcttagccc gggtccaacg atgggcatcg
     9361 tcttgggcgg cgccaggccg gtggcctggt tggcttcgtg cagttgaatc cgactttgat
     9421 ggcatctggg gcgggtctgt ccgactgttg caggtggaac caggtttcga tggcttatgc
     9481 gctgctggtg aggctcgaca tgccatccaa tcgggtcagg cttgctcgcg acggagccag
     9541 gtcttggata tgccatcaaa gtcgggttag ccgcgattcg gtgccaaggc tggcgctcgg
     9601 tgttggctct gccgcccggt agcgcgggca ccggctacct tggaatgccg gaacgcgtta
     9661 ggagcagtct tggtcagcca acagaacgaa ccgcccgaga aggaggagaa ggagcccatc
     9721 aaggtctcct tctcgctgcc gcaattgatc ggcggcgcgt tggcagcggc aactgcggcc
     9781 gccgtcggct ccagtctggg cgtggctggg acgatcttcg gcgcggcggt ggccagcgtc
     9841 gtcggcggcg tgggcggcac cctctacagc gcgggtatcg accggacgca tcgcaaggtc
     9901 gcatcagtga tcacgcggac ggggcccgtc ccggtcgaac cggccgacgg tgtcgacctg
     9961 accctgttgg acgtgaccgc tggcgcgcct ccggtcgcgg gatcggcttc gggatcggct
    10021 tcgcggtcgg ccggtggcga tgacccgtcc ggacggaaga agcggatctg gaaggtggcc
    10081 gcgatcactg ctgcggcgat cttcgcgatc tcgctggtgg tgatcaccgt cgtggaactc
    10141 ggcctgggcc gcgcattcgg tggaggtgag gggacgacgg tctcgcaggt ggtccgcccg
    10201 aaggcgaacc cgagcaagtc ggtgagcccg accccgaagc cgactccgtc cgagtcggcg
    10261 tcttcgtccg cgccggagcc gagcccgagc gagtcggtga cgccggctcc gtccgagccg
    10321 accgagtcga gcgtcgtgcc cactccggcc gcgacgccga cggtcacggt cacccaggtg
    10381 gcaagcaccg aagctgcggc ggtggagccc accggctagc tcgccaggaa tccctcggtt
    10441 ggtcgagcct ggccctgagc ttgtcggagg gccgaggccg ctagttgtcg aagctgttga
    10501 tcatgaactg cgcggccctg gtgacgtatt cgcggaactc cgccagttcg gcctgcggca
    10561 gctcgagtaa gtcgagggcg gcgttcatgt ggcgcaacca gcgatcgcgc atgtccgggg
    10621 tgacctggaa cggcgcgtgc cgcagccgga gccgcggatg cccacgggtc tgggaatagg
    10681 tgtgcggacc gccccagtac tgctccagga acagcagcag ccgctgcttg gccgggccga
    10741 ggtccgcctc cgggtagagc gcgcgcagcg gctcgtccgc cgcaaccccg cggtagaact
    10801 cgtcgatcag cttcacgaac gtctcgtggc cgccgacccg gtcgtagagg gacgcttccg
    10861 caggcatccg tccattcttc ccgggcggac gcgtggacgc catcgcgagc gtttcacacg
    10921 ccttctgccg aatcgccgga tggaactgtg atggcgcatc aaggaactgt ccccagaatc
    10981 atcggtgcca gtgagagccg agtggcgata cggaaaggaa ccgtcccccg agttgctgcg
    11041 ggcttggctg ccgacacacg gcccccggat cagcccagat aggacgcgat cccgtccgcc
    11101 agtgcgaccg cggcctgggt gcggaacttc gccgacttga gtagggctgc gtccttggga
    11161 tggcgcatat tgcccatctc catcatcacg gcgacccgct tcgacaggtt caacgtggca
    11221 atgtccgagc gggggctgag cgcggtgccc ttgccgatat aggtcgaacg cggcatcgcg
    11281 gtcccggtct ccagcgcgct gcgcagatcc ttggcgaggg ccttcgattc ggcttccagg
    11341 gcggcgccac cgaccatcgt cgtgctgatg atcacgtgga acccgcgcgc cttcttggaa
    11401 tagctgccgt cagcgtgcag cgaaatcagc acgtccgcgc ctcgcgcatt ggccagtgtc
    11461 gagcgaaggt ttacgcacgg tccctggctg gagtcgtccg agcgggtgag cagcacctcg
    11521 gcgccgcggg cgcgcaactc ctcggcaagg gtgttggcct gcgcccagtt gtaggcgtgt
    11581 tcggcgtagc cggagttggt tgccgtgccg gaggagttgc aggccttttt cctgccgttc
    11641 ccggacggca ccagcttgcc gagcttcttg gtccagaccc cgttgtgccc tggatcgatc
    11701 acgatcagcc tgccggcgag ggtgccagcc ggcatcgcct cggtcgcaga gtgtgcgcgt
    11761 aggtcaaccg ccgggctggt gtcggggacg gccgcgccga gcactgcgct ccggagcgag
    11821 ggggcggagg ccgacgtgac cttcggggcg tccggcgtcg gcgtcgtgca acccgtcagc
    11881 gcaactgcgc acgccagcac gacggacgca gatcgaagcg gacgcagctg gtgcacccga
    11941 ccagtatccc ctcttgcgcc acccaagtaa tctgagcaac tctcagcttt ttctaggctt
    12001 ttcctcagtt tggacgcatg gttgttccgg cagaactcaa gaagtagcta cgccagcccc
    12061 tgacgagctg gtcgcgtccc cccgacacga ttcgaccggc tgcgcgttcg cgtccggaat
    12121 acccggtcac gctgttgcgg ttggcacagg taacccagtg agaggattgc cgtgaccgag
    12181 gagttcagtc acaaccaggc cgagagccgg ttcgaggtgc acatcgacgg cgagctggcc
    12241 ggcgagtgcc actaccgcct caacggggac gtggccgtgt tcgaccacac cgtggtgcca
    12301 cccgagttcg gtggccgcgg gatcgccgga cgcttggtca cccatgcaat ggacgaggtc
    12361 cgcaccgccg gcgcatggaa ggtgcggccg gcctgcagct acgtagccgg ctggttcggc
    12421 aagcatcctg agtacgccga tctgctggct tgaaacgagg gggcgtgtgg cgggtgagca
    12481 attctgacgc ttgccgccac ctcccgtccg agcggacgat tcggccaaga ccgcattttg
    12541 ccggggggtc gattgtccag tcagccgatc atcatcgacg ccgagcagac gcgtcagcgc
    12601 tccgaggcgt gcctggccag cggtgacttc aaggcagcgg acgcgggcta cgccgaattg
    12661 cgtcggctgg gcgcgctttc ggcaagcgat gccggcaagt ggctgagggc agtccgtgcg
    12721 ctcacccagc tcggcgagct tgacgagaac gaccagcgac ccagcctgga agagctggtc
    12781 gccgccttcc ccgacgacca ggatctgctg gagttcgtcg ctgccgaagt ctggcagcgc
    12841 gccgagtccc acgcggtccc aggcgaagag gacgccgaaa tcgcccggcg agcgtggcag
    12901 gccgccctgc acatggccgg ccccgtccca gaatcggacg cggccggcgt ccgcctgatc
    12961 agactggttg accgcttctt ccaagccgga cgccgccagc agacgccgat cggcagctat
    13021 cccgagtacg agcaggcgct ggtcaggttc gccgccgccg ggccggaatc gacgctgcga
    13081 gcagcagatc tgctggccgc tgagagtcct gtcctcgcgc tggaactggt tgcgaccagc
    13141 gagatcagcg cagccagcgt gctggtgcgg gcggtcgcgt ccgccgagat ggaagaccac
    13201 gtgggcgcga cgtcattgtt cgacgagctg acagcgcgct gggggcaggg cttcgagacc
    13261 ctggacgtgg aggaccgttc ccgctgggtc cagtcgatga ccgacgtcgg ccggttcgct
    13321 gaggccgaag cggcttgcct ccgcacgatc gagcagatcg agcacagccc ggagaccttg
    13381 gccggaatgg acggcgccca agaccactgg cgccacgtct accacctgct ggcctaccgg
    13441 ctggccgcgc agcagggccg ctaccgcgaa gcctgggacc acttgcggcg cgccagcacc
    13501 gtcctggacg cggacctctc gccggagttg cgccgctccg tcctgcgggt gcgcatcctt
    13561 ctcgccgatc gggacgaggc cgaaggcatc ctcgccgaac tggcagagct ggccagctgt
    13621 gacccctacg acaccgcgta cgccgaagcc tgcctgtggg cgggcacgca gtggggcacc
    13681 ccggacgcgg acgcgtcgca ccacgcctcc ggcggcacct tcagtggcgc tcgctacgcg
    13741 ggacaggccg ccgcccgcgc cctgctggct caggccggtc cggaagtgag cagccgccag
    13801 ctgatccggc tggcgctgct ggccggcgat gacgaccgcg cacaggcgtt gttgaccgcc
    13861 gagccgctgg ttgcgtccga tccctggccg gcgaaagtac tggcagcgct ggtggcgctg
    13921 cgccagggcg acgacctcac cgccaaccgg ctgatcgccg aagtcatgcc gcagcgtcgc
    13981 cacgacctcg acctgcgggt gctggacgcg caggcttccc tgatcagcgg tgactacaaa
    14041 gtgtcgttgc gcgaggcgat ggcgatcacc gacagcgttg gcgtacacat cctggcccgg
    14101 gtgatccggg cagagagcga gttcgaggcc gcgctcgcga tggtggcgga cgaggacgcg
    14161 tcccacggtt cggtggagaa cgcccagcag ctgatggcgg ccgtctccga ctaccggacc
    14221 gtggccgacc tgcagcgcga cacgatcggc tatctgcgca ccgcagcggc ccccgacgct
    14281 gtcgcgatcg gatcggagcc gctaccaccg cggttgttca ccgaggtgtg ccggcgcggg
    14341 ctgcacgccg cgatcctggc ccaggaggcg ttggaccggc tcggcctacg cggggaccgg
    14401 cagttggtga ccgatgccca ggacctggtt cagcacctta ggtcggtaac ccgtccgtgt
    14461 tgccggacgt ggggctcgcg accgtcccgc ctactgcacc aggtgcggca cctggtcgac
    14521 cgggacgagg ccacccggct ggcactgctg atgatctcgc accgctggtc ggcttggcgc
    14581 cggcggctgc agaattttgg cttcatcggg ctcggcgcgc tggtcacgtg ggcggggctg
    14641 acgaacgtcc tgcccggccc cgagtcggac acgatccgcg cgctggtgct cggtctcggc
    14701 gtcctgctgc tgctgatgcc gttcgcgcgc agcctgaagg tgggcgtggt cgaactcagc
    14761 cgggacgcgg acgccgcccc gctgagcgga cgctcgaagt ccctgcgcac ctcccgcctg
    14821 ctgctgcgag ccaaccatct cgggacgttc ccgctgccgt ccccaccgga gaagggccgc
    14881 cgggcacacg cccagtcgcg tgattgagcg tcccctgagc tacccggctt ttgctaccga
    14941 aattggcgtt tgccagcgaa attcccgtag caaacgccaa tttcggcagc aaacctggga
    15001 atgcgaacgc agccatcggt gcggtcgtcc cctatggaac ggtcctcgca agctcaggac
    15061 aaagctcgtc agggtccctt tctcagccga gcagcgtctc gggagcgttg gccaccgcga
    15121 cctcgacttg gcggcgatgc cgtgcgctca tccggggtag ctggtcgagc gggaagaagg
    15181 cagcggcgga cgcttccccg tccgccgggt acggctcgcc cgacgtccag cggcagcgga
    15241 agacgtggtc gatgtactgg ctctggtcgc cgttttcgta ggtgatcacc tcggtgacgg
    15301 tgagccagac caaccgatcg atctccgcga ccacaccggt ttcctccgcg atctcgcgga
    15361 tcgcagtgtg gtgaggatgc tcgccgggat cgacgatgcc ggcgaccggc gtccacgcgc
    15421 cgttgtcgga tcgtctcacc aggagcacct cggacgcgtc cgcctgctcc cgcaggacca
    15481 ccccggtagc accggagagc caaagcggca tgctgccgac atggttgcgc aactcgatga
    15541 tgaagtcggg tgtgggcacc ggaagagcct acgcctgcgg ctgggtatcg aacacgatcg
    15601 ccgcgaacac gatggcgcca gggccggagc accgtccccc cagatcagtg atcccggccc
    15661 tggccgcctc gagcatcagc ccccatgtag ctgagcgctc ttcgagttcc cccgagcgtc
    15721 cgacactggg ttcgagtcgt ttcggacgga ttctcggcga ggtcaccgtc gactggtgtc
    15781 cccgaccacg tgccccccgt acgggcgggt catcgatgag tcgcaggtgg tttgaccacc
    15841 tctgggctta accctaaagg cgtactgaac ccgtggggaa gcctctgact accggttttg
    15901 cagatctgtg cggagtagaa gtactcatcg gtctacgctc ttgacgtgtt ccgaacggaa
    15961 gcgtccccgg gtcctgcggt gctggtgcgg cccatgcgga ccggcgatgc tgcgggcgtg
    16021 ctggccgtcc agttggccgc attcacgatc gagtccgagc tgtacgacga tcccgaattg
    16081 ccgcccctgc tggagccgca cgcggacctc aaccactggc tggacgcggg cgggctcggc
    16141 ttcgtcgccg aggcggacgg acgcctggtc ggctcgatcc ggatcgaact ggacggcgac
    16201 gtcgtactga tcagccggct ctcggtcgca cccgaatacc agcgcagggg catcggcgaa
    16261 gcgctgctgc gccacgccga gtccgtgacg cccgcggtca ccgcgagact gttcaccgga
    16321 cacttgagcg ccgcgaacct gcgcctctac gcccggctcg gctacaccga ggagcgccgc
    16381 cagccagtcg acgccaaagt cacccttgtg caccttcgca aggacctgcg cccgtccgac
    16441 taactgggct atccgagcga agttgaagag gggatggacg gcgggggttt cgacagggtc
    16501 gaccggttgg ggtcggctag ccagctaccg ggggctcgac tggggggagt ccttcgcggg
    16561 cgaggtcctt cgccaacgcg gacgcggtat cgctgaccga ttccaggggc gagctgaacg
    16621 gctgctcgtc accgatgcag acggttccgt ccggctgggc gccggtgagc aggtaagcgt
    16681 ccacgcggga ggtgacgcac gcggacgtcc cgtaggcggt gtgcccccac gagtcgctgc
    16741 tcagcaggta gctgttcggc atcagccggg cggtctggac ggcgttgtcg tagttcgtgg
    16801 ccggatcctg gtagttcccg accagtagca ccggtgcggc cgtcggctgg atgaagctgc
    16861 cgcggaacac gtcttcgtcc ttggccttcc agtactgggt ggcgcactgc acatccgacc
    16921 aggcccacag cctgccgaaa tgcggcacgg ccgcgtccgc gcgcgtcgcg gccaactgcc
    16981 aggtgcccgg ccatcgcggc tcgcgtgagt cggtgcagat cacgctcgag taggcgtcca
    17041 gctcgttgtc gtaggtaccg atcaggaccg actgggtgcg ggccgcgcgc tggtacgact
    17101 tcaccagcgt catcaggttc ttccccgcgc gtcgtagcgt tgcgctcgag ccggggtaca
    17161 gcacgtcgtc tagccgggcg gtgagctcag ccacatagtc catcccggac gggtagtaga
    17221 gcaggctgag caccgccgag atgtactcgg tgtaggtgaa gcggaacgcc ccaccgtcat
    17281 cggcgacgtc cacccagccc ctgatgcgca gttccgccgc cacccggtcg aaatcggctt
    17341 tggggtcagc cagtgggcag tactgcggac ccttcgccgc gcaccgttct agcccggtct
    17401 gcagcgcctg ccaggcgccc tcggccgccc ggatccggat ggtggtcggc gacccggcag
    17461 tactcgagtt gccggcccac gccgtcgggt cgaccacgcc gtccagagcc atcgctcgga
    17521 accgatcggg gaacaggctg gcgtagacct cgccgagata ggtgccgtag gagaagccga
    17581 gatagctcag cttggcggac ttaccgccca gcgtgcggcg cagcacgtcc agatcgcggg
    17641 ccacctctgc ggtcgacatc gcggacgcca gcttgcgggc gcgcttggag caggccttgc
    17701 ccagcttctt cgccgacttg aggtagagct cttcctgcgc ggcgtcgacg gggaaagcga
    17761 ccgacatgcc ggacagcgcg gacgtctgct tcgccgcact gctgaagcag cgcacctggg
    17821 tggagccgtt cgtcccccgc ggatcgaacc cgacgatgtc gaacttgttg aggatctcct
    17881 gggaaagaaa ggtggaggcc gtgcgggcca gcgccacccc ggaaccgcca gggccgccgg
    17941 ggttgaggaa cagcgtgccg acccggttgg tcggatcggc cgcgggcacc ttgaacagcg
    18001 ctacccgcac tttcgcgccc ttgggcttgt cgtagtccag cggcaacttg accgttgccg
    18061 agtacccgct gccgtcactt gccttgtgcc acttcagctt ggatacgcgg acgcggtcga
    18121 cccgtttggc ttccttggcg gacgtggtcg cagccccgac gttcgcggcg agcccggtct
    18181 tcacctgctc ggcgatcgcc gcggcgggag cagcgccggc cggaccaacc gacagcaggc
    18241 tcgaaacgag cccgctgaca atgattggca cgagtctatg gcggcgtctc cccgattgca
    18301 tgcgcctctc ctggacgttg ctgaaccgat cgcccgatga gatcccggcg ccgccgagtg
    18361 acggtggatc cgagcgaggc agcggcctgt tcggccaaat atagcccgtg ggtgcgtacc
    18421 gttcaggcgg ttgccagttc tcgtccccag gctgtggccc ggccgagttc cccgtccgcg
    18481 agcgggccct ccattccggt gacgtagaag gacggcccga tcagggtctc cgcgccgcgg
    18541 tcgcgcaagg cgatggccgc cgcccgcgcc gccgtcccga gacgctgccg ctcctggatc
    18601 gaggtgtcga acgtggctac ccgaataccc ttcggcaact ccaagacctc gatccactcg
    18661 cgcacgccgg tgcgctcagc gacggtcgcg ccctgcccgg ccgcgtccgc acgcgagccg
    18721 gcgttcggca tcgagagagt gtgggtcggt gcgccgatca ccagcagcgc cacctccgca
    18781 ggcaagctgt ccggggcgtc ggaggcgtgc atcagttgca cttcccgagc tcccagcacg
    18841 tccgccagcc cgccgctgat ggcctgggcg atggcgagag tgttcccgaa gatcgattcg
    18901 gtcacgatga gggcgctcat tagggcgttt cctttccacc ggtcacctta cgcacgctcg
    18961 ccgcagggtc cggacggtgg tcagaaatcg acccattgat tgaatggtca aacgatttaa
    19021 catccggccc gctcgaggcc ttcgtccagg ccgatgtcat caacacccat gcgctcaagg
    19081 cgaccggtgg caagacctac gcccagctcg ataaggagga tccgctgcgg gccaccgcta
    19141 tgaacggctc cttcctgcgc tcctcgctgt tcacctcggt gatcgcgttc ggcgttgccc
    19201 tgttcgcgat cggcgtgggc gtgctggccc tgctgcgttc agtggctggc tcagccttct
    19261 acttccacga cctccgcgga cgcggcgtgc ttcttgaccg actcgatgcc atcgcggcag
    19321 ctgcgctcgg tggcgtagcc ctggccgctg gcgatgatct ggccgttgct ggccttcagg
    19381 cgccagcggt acttgccggc ggcgtccggg tagatctcga acttgcccat gatctctcct
    19441 cctcaggcgc ggacgtcggc gtccgctcct aaggaaggtt attgaagagt tggcgtcgcg
    19501 gtgccgagat tgctcaggcg agttgcagcc aggccgcggc atccgggggt agaaggccgg
    19561 ccaccagctc ggcgctggcc aggagcacgg acgcgccgtc cggcagtgcg agtggcttgg
    19621 cgccgaagtt cacccagcag ccgaaaccag gttcccgggt gaacgccaaa gcgtccgcac
    19681 cggcctcgac ccaggtcagc gtgccgtcgc cgagcgctgg atgctcggcg cgcagcgcaa
    19741 gcgcgcggcg gtacaggttc agcgtggacg cctcgtccaa ggcttcgatc tcggcgctaa
    19801 gcacgctcca gccggacggc tggggcagcc agggcgcggc cgacccgtcc ggtgagaacc
    19861 cgtagttgct accggacgcg gtccagggca gcggcacccg gcaaccgtcc cggccgaggt
    19921 tctcatagtt gttgcccgcg accatcggat cctgcatcgc ggccagcggg atgtcctcca
    19981 cctccggcag gccgagctcc tcgccctggt agatgtaggc gccaccgggc agggccaggc
    20041 tcagtagtgc tgcagcccgg gcgcgtcgcg tcccggcttc gaagtcggcc ggcaggtcga
    20101 tgaagtggct gaggttgcgg tccgtcggac cgaccagctc ctgcggtcgg gcgtaccgcg
    20161 acaccgggcg gcagatgtcg tggttgccca gcacccaggt ggtcggtgcg ccgaccccgg
    20221 cgtgcgaggc gagagtggct tcgatcgtca cccgcatcgc ctcagcgcgg aacgggctcg
    20281 tcaggaactc gaagttgaac gcggtgtgca gttcgtccgg acgcaggtac gcggtcagcc
    20341 ggtgggtgcg gcccacccag atttcgccgc agaagactcg tggcggatcg taggagtcgg
    20401 ccacccgccg ccactcgcgg aagatgtcgt ggacgccggg acggtcccag tgcgggtggt
    20461 ccaggtcctc gggcgtgtcc gccggcagcg ggaagggcag gccgttgacg tcggcgagac
    20521 cggcgtcctt gaccaggccg tgggccacgt cgatgcggaa gccgtccacg ccgcggtcga
    20581 accagaagcg cagggtctcc tcaaactcag cctggatgtc ggggtggtcc cagttcacgt
    20641 ccggctgctc gggggcgaac agccgcaggt accactggcc cggaaggccg tccgcttccg
    20701 tgacgcgcgt ccacgccggg ccgccgaact cactcatcca gtcgttcggc ggctcgtcgc
    20761 cggccggtcc gcggccctcg cggaagatga accgctcccg ctccggcgac cccgggccgg
    20821 cttcgagcgc ttcggtgaac cagcggtgct ggtcggaagt gtggttgggc acgatgtcga
    20881 ggatcacccg gatgccggcc gcgtgggctt cggcgatcag tgactccgcg tccgccatgg
    20941 tgccgaagac cggctcgatc gcgcggtagt cggaaacgtc gtagcctgcg tccttcatcg
    21001 gggacggata ccagggattg atccagatcg cgtccacgcc gagggcgctg aggtggccga
    21061 gcttggcgcg gatgccgttg atgtcgccga ccccgtcccc attgctgtcg gcgaagctgc
    21121 gcgggtagat ctgatagacc accgcactgc gccaccagtt ctcgtcggcg gtcggacggg
    21181 tcgtggaagc ctgagtgctc atgggtgcct tccggatgtg ctcctgctgg gaagtaccag
    21241 cctacggccg tccggaaagc gctcgactgt ggaatcctgc agcacagacg gtgcacgatt
    21301 ccacagtccg ccccgggcct cgtgacgcgc tacttcttgg gcttgccgaa gtcctgcgtc
    21361 cagtaggtgc cgtactttcc gccttcggcg tagcccacac cgagctgggt gtagctcttc
    21421 ttcatgatgt tcttgcagtg gccggggctc ttcagccacg ccttcaccac ctgggcgggc
    21481 gtgtcgtagc cggccgcgat gttctcaccg gcggacgagt accggtagcc ggtggctttg
    21541 atccgggtga acggcgaggc gccgttgcgc gagtagtggc tgaagtagtt gccgctggcc
    21601 atgtcggtgc tgtgcgcaac cgcggcctgg cccagcgagt cactccagac gagcggcttc
    21661 accttcttgt aggtggtctt gccgcacttc cgcttggccg aacgggcctc gttgaccaga
    21721 cgaagcacct cgttatccat cgaggacact tcgtacttga cggtgtagcc actggtcacg
    21781 acagcgggga cggcagcagc agtagcgctg gcggcgttct gagtgacggg aagggtgatg
    21841 aaggctccgg ccaggattgc gaccaggcgg aacggcagag cacgcgggag ggggtgcatg
    21901 cgcgcgactt tctatgtgca gttgctcgca gccgaaactg cgaactcccc cttgccccgc
    21961 cccctactct gccgcagacc tgtgaaacgc accagcgatt ctcagaactt ggccaatttc
    22021 gtcccagatc tgaacggagg cggtaagcgg ccgcaccaat tgtggtcaca attgggctca
    22081 tggcgggccg ggcggcaaag cgatctgagc agggtgctca ggcagctgcg cgagcgtttg
    22141 ggcaagccaa cgccagtttc gtgagcgaac ggctcgcggt cggcggtgat ctgtccgcca
    22201 acttccgccg cgcgcggtcc cagctcgacg agctggtcgc cgcgggcatc acccacatcg
    22261 tcgacctccg ctccgagtgg accgacgaga cgctggttgc cgagtgggca ccacaggtgc
    22321 gctacctgca ccatccggtg gcagacgcgg ggcaggtgat cggcccggac tggttcgccg
    22381 agctggtggg ttgggtggac gatgccctgg atgatcccca ggcgaaggtg ctgctgcact
    22441 gtcacatggg cgtgaaccgg gccccgtccg ccgcgctggc ggtgctgctc agccagggtg
    22501 tcgaactgcg gcctgcgctg aacgcgatcc gcggcgcccg tccggtcgcg atcatcgact
    22561 acgcgcgaca cgcgctggac tggcacctgc tcacgtcggg ggcggacgcg cgcgcccggc
    22621 ggaacgcgcg tcgccggctg ggccgctgga ggggctcgaa ccagctcgac gcgacccagg
    22681 tgatccgggc catccgcgac accgagactg cagccaatcg ctggtggttg cggctggacg
    22741 aggcaacgcc ggacctgctg gcggagttgc tcgaatcggc tggcgaattg gccatcgggc
    22801 tgccggtgac cgccgaaccg cctgaactga gcctgttcga cgaggtcttc ctgatcaccg
    22861 tccagggcct ggtgggacgt gcgctggtgg ttggcccacc ccgcgccggt gagtcggacg
    22921 agaagttgct gccgctgctg gcgcgggaac tgttcccgcc ggtggcgctg accctcccgc
    22981 gccgggtcgc caactggctg aggggcgcac gccccaacct tcgtcggata ccggacgccg
    23041 agtaccgcgc gctcacgctc gccaactgaa cggccggtga ccactagccc ctggtgggct
    23101 cagggagcgg tctagagttc gacggtctcg agcaactcgg ccggacgcgc gggaatgctg
    23161 agtcggacgg tgatttcccg ggcgaacgcg gacgcggagt cttccgtccc gtgcaccgtg
    23221 tacacctgct gcggccgggg ctcaagagcg cccagccagt cgagcaactc ggagcgatcg
    23281 gcgtgcaccg agaactcctc gtcctgcagg atctcggcgc gcaccgccac gttgcgaccg
    23341 tgcatcttga ggctgcggac gccgtcggcc aacagccggc cacgtgtccc ctcagcctga
    23401 tagccgacca gcacaaccga gtgccggtcg tccggcagca tgtgctccag gtggtgcagc
    23461 acccgcccgc cggtggccat gccggaggac gaaatgatga tgcacggcac ctttgggtcg
    23521 ttgagcttga tcgactcctc ggtactgcgc acctcgcgca gtccgggcag gtcgacgaac
    23581 gacctcgggt ccaggtcggg acgcaactcg tcggcgtgcg tggccgacgt gtacacccgc
    23641 agcgctgcca gcgccatcgg cgagttcacg aacaccggca gttccggaat ccgaccggcg
    23701 tgcagcaggt cggtcagggt ctgcagcacc acctcggtgc ggtccaccgc gaacgccggg
    23761 atcagcaccg aaccgccgcg cttggccgtg cgtcggatca ggtcggccag cggctcgtgt
    23821 tccgggccgg acggctcgtg gtgctcccgg tcgccgtagg tcgactcgat caacaccacg
    23881 tccgcccctg ctgggatctc ccgcggacgc agcaccggat gggtggcccg gcccaggtcg
    23941 ccggagaaaa gcacggaccc cgtcggcgtg cccagccgaa tgctcgccga gccgaggatg
    24001 tgcccggccc gcgtccatgc cgcagccaac ccgtggccca gcgagacctc ggtgtcgaag
    24061 tcgatgaccc gcatccggtc gagcgcacgc tccgcgtccg cagcggtgaa cagtggcagc
    24121 ggcggctgat gccgggagta gctgtggctg gccgcgtgct cggcatcccg ctcggcgagg
    24181 aaggccgcgt cccgcagcac gatctcggcc aactcgcagg tcgcctgggt ggccagcacc
    24241 tcgccgtgga agccgttgcg caccagcacg ggcaggtagc cgacatggtc ggtgtgcgcg
    24301 tgcgtgatca gcacggtgtc gatggactcc ggagccgtag gaaacggcgt ccagttgagt
    24361 tcgcgaagcc gcttctcccc ctggaacacc ccggcgtcca ccagaatccg gcgatcgtca
    24421 gaattcacca gatacttcga gccagtgacg gtgccggcac caccaccgaa ggtgagtttc
    24481 actgccatgc ccccagcttg gcactacgcg tcctgattag gagtttcttc gacaagatcg
    24541 gccgaccgcg ccagtccagc cgcctagtgg tgaaagtgaa ccgagggcgt ccacatcgtg
    24601 aacagattcc atcaatcgat aaagagggtt gatgccgtcg cgtgccgcgc cgtagcctgt
    24661 cggagtcgag catccctcca gctcgtctgc gcccgaggaa ggtaggcaat ggtgccctat
    24721 cgcaatcccg ccctgctcgt ttcaatcgct ttcttgctgg ccgttcccgc ggtcgccgaa
    24781 gccgcgcccg ccggcaccga cgacacgctc accacggctc gcagcagcgg ccgggtgagc
    24841 acgacgctca ccccgcgagt gctggctggg ggcgccttgg tggatgtgat ggtgcaactc
    24901 gaagccgacc cggtcgcggt tgcggaggcc aaggccgagc ggacgttcac cgcggccgag
    24961 cgcaccaccc acaagaacaa gctgcgcaag gcgcaggatg cgatcaccga cgacatcgcc
    25021 ggcaagggcg gcaaggtgat ctcgcggatg cagtcggcct acaacggcat gcgggtgcgg
    25081 atcaaccgtt cccgggcgga cgcgctcgcc gcgctgcccg gcgtgagcgg tgtgcacgcg
    25141 atcacggcga agaccctgga cacctcggtc tcggtgcctt acctcggtgt gccgtcggtc
    25201 tggcaggaca ccggcttcac cggcaagggc gtgaaggtcg ccatcatcga ctccgggatc
    25261 gactacaccc acgccgtttt cggcggtccg ggcacggttg cggcgttcga ggcggctgca
    25321 gccactgcca cccaagccgc gaatccggag tggttcgggc ccgacgcccc gcgggtgaag
    25381 ggcggctggg atctggtcgg cgacgcctac gactcggacg ccgagccggg cagtccggca
    25441 gccacgcccg tccccgaccc caacccgctg gactgcaacg gccacggcac ccatgtcgcc
    25501 ggtatcgccg gcggctcggg cgtgaccgca tccggtgatg tctacacggg cgcctacaac
    25561 gccaagaccg cgaagacaca attctcgatc ggcccgggcg tcgcgccgca ggtcgacctg
    25621 tacgccgtcc gcgccttcgg ctgtgaaggg tccagcgaag tggtcgcgga ggcgatcgac
    25681 tgggcggtcg agcggggcat ggacgtaatc aacctctcca tgggcagcac cttcggccgc
    25741 gcggatgacc cagatgcggt cgcggccagc aacgcggtcg gcgcgggcgt ggtcgtggtg
    25801 acgtccgccg gcaacgaggg cccgaacccc tacctggccg gttcgcccgg agttggtgag
    25861 ggcgtcatct cggtggctgc cgtcgacagc acgcccagct tcccgggagc ttcgcttagc
    25921 ttcgctgggc agcagttgac cgcgataaac gcgaacgcgg ccgatctgcc gggcacggct
    25981 ctcaccgtgg tgaacgtcac ggacgacccg gacaccgcgg aggtcgaatc gctcggttgt
    26041 tcggtcgcag ccttcaccca ggccggcatc gtgccggacg ggcaccagat cgcggtcgtc
    26101 gagcgggacg gctgctcgcg tccggcgaaa gccatcttcg gccagcaggc cggcgcggcc
    26161 gcagtggtga tgatcaacac gatcgcggac ggatacccgc cgctggaggg tgagatcacc
    26221 tacaacccgg acgacgggat gccttatcag gtgaccattc cgttcctcgg ggtacccaac
    26281 acggcagcgg cggcactggc cggcgcggag ggcgccgcga tgacgatgac cgcgaccacg
    26341 gtggccaacc ccgcgctggg cgagatcgcg gccttcagct cagccggtcc ggtcaacggc
    26401 agctcagagg tgagcccgag cctggccgcg ccaggggtct cgatcacgtc cgccggcatc
    26461 ggcaccggtg acggcacgac ggcgatgtcg ggcacgtcgc aggccgcccc gcacgtcacc
    26521 ggcgtggcgg cgctcggcgt gcaggcgcat ccgaagtggt ccgcgcagga catcgccgcg
    26581 accctggtga ataccgccga cccggcgaag gtcagcggct attcgctcac cctcgccggc
    26641 tccggcctgg tggacgctgc ccagaccgtg accaccgagt ccttggtcac gggcgaccgg
    26701 taccggacca gttccggcgt tatggccgag ccgtcgctca gcttcggctt cgccgagccc
    26761 tcgctcacct tcctgggcac caagtggatc acgatccaca accgcggctg cgaggcgatc
    26821 acctacaagc tcggcgccga agccagcgcg gcctcgcgcc cggcgagcct gaagttcggc
    26881 gcaaagaagg tgaaggttcc ggcgcgaagc aaggtgatgg tgccggtgac gctgctggtc
    26941 aacgcggcca agatcggatc gtcgctgacc gacactcagg ccggcttccg tgaggtttcc
    27001 ggcaacatcg tgctgaccac gggtggtgac gagttgcggg tgccctacct gctcgtgccg
    27061 cgcgcacgcg ctcaggtcag cgccaaggcg ctggacaggt tccccaagcc ggtcgctgcc
    27121 cccgaatacc tggcagcgga cgcagcagac gcagcggacg agtcagcagc cgaggtcgag
    27181 gccaactcac cggcggagtc ggtcaacgtc gtcgagcccg tggaagacct gggtgcagac
    27241 ccggacgcag aggtagctgc cggtgagccg gaatcctccg ctggtgcgga cggcgtcgcc
    27301 gaggccgccc aacaggtgac cgacgatccg atcgaagagc cggtggcgga agccgtggaa
    27361 gccgtggaag agatcgcgcc gaccgcaccg accgcgccca acagcacggc gcagaccacg
    27421 acggtgcggg tgtcgctgcg caacccgctc ggtgcggtgg acgcgtccgc ggatttctac
    27481 acctgggggc tcaccgaccc gaaggatccg tccgcccgcg gggggtacga cctgcgcgcg
    27541 gccggcgtcc agtcctatgc gaacggcgcg gacacgctgt tgctgttcgc ggtgaacaat
    27601 cactcccggt ggtcgaccgc ggccactcag gagttcgacg tgtcggtgga caccaacggt
    27661 gacggcgctg ccgactacgt ggtgctcagt tatgactcgg gctacatcgc caccggcacc
    27721 tacaacggac tgaccggggt gttcgtctac gacgtggccg ccggagcgat gtccgcgagc
    27781 ggctacaacg cggtggcgcc gaccgactcg agcacgatcc tgctgccggt gaacgcgtcc
    27841 gcgctggggt tgagcgcgga cgccggcgac ttcagctaca ccgtcgacag ctacagcatc
    27901 ctggatccag tggccggtga cagcttcggc gcgtgggcga actacaaccc gtgggcgaag
    27961 gccattggcg attcggccta cgtcaccgtc cggcgcaatc ggacgctgaa ggtgccggtc
    28021 gaggtggacc cggtggccct gggcacgcag cggccactgg gcgtcatggt ggtggccttc
    28081 gacaaccagt ccggtgcttc ggaggccctg ctgctgaagg cccgctgacc cacgctcccc
    28141 gagcccaccc acgctccccg agcccgacga aggggcggtg gctcaggccg gccggcagag
    28201 cctcggctcg cccgagcgtc cgaagcggta gtcgtattcg actccggcca cgctgacgac
    28261 cacctgcatg ccgggcacct gagcctgggt gtacagctgg ccgggcttgg gacagcccag
    28321 ggaaccgtca ttccaggtga cccggcgcga agtcaccacc cggacgtccg tcgtcggaac
    28381 ctgacgggcg gccagatcag cgaggatcgc gtcccactgc tgtggggacg gggtggcgac
    28441 cgtcccggcg ctcggcgtca ccgacggcag cggggacatg gtctgcagtg gtggtctttc
    28501 gcccatctcg cctcggctcg cgcagccgca cagcagcact gccatcgtgc tgaccagcgc
    28561 agcccggacc atgtcgcctc ctgggtcaag cgtacgagct cggacggttc gagatcccgg
    28621 ccaacgcccg gatgacgtgg cctgtggaca atgggatgac gtggccttgg ctggcatgcg
    28681 cccagcctgc catgccagct cgtcagcgcg acccggtgag cgccttcagt tcccgggccg
    28741 cctcgcggga cgcgtccgct tcgccgagtt gccagcgcag cccgaccagc gcttccagcg
    28801 tcgtggcgag ttcgtcccgg tatgcctcca gcgcggtcag cggacgcagc aacccggcca
    28861 gccggtccag gttgtgggct cggtcggccg gggattcggc cgcggcgttt gccgcggagc
    28921 gggccagggt cagctcgtcc tgcacttcgg ccgctgcccg cgcggccagg cgcgcctgaa
    28981 gttcggcctg ctgtcggtgc tgttcggcgg cctgccgggc ggccctctcc gccttggccc
    29041 tacgctcggc cgctgcggcg gcttccgtcc gttcccgctc ctggcgggcg gctgcctcct
    29101 gctgagccgc tcgcgcagcc gcggtcgccg cggccagttc gtcctctgcg agctgtcggg
    29161 cgcgttcggc agcttgatca cgatggcggg tttccgcggc ttcggcgtcc cgtcgcgcgg
    29221 tcgcagcggc ctgggtggac tcctgggccg cacgctcgga cgcggcatag ccgctggtca
    29281 gatcgcgatt gctgagctca gcggacgcat cgagccgcgt ccacgtcacc ggccggcctg
    29341 gtgttggcgg ggccgtggcg accccgtcgg cggacgcgaa ctcgtccgct tcagcggcgc
    29401 gcccagcccg gcgcagggca gccgccagcc aacgttggcc ttcggcgtgc tcggcaccca
    29461 gacggtccag gagggcgagg tgggcgcggc gcacgcccag cgcgaaatcg acctggccgg
    29521 ccgactccag ccgcgacgca tacggttcgt aggtcgcggc cgcggtggcg gcagcggtcg
    29581 ccaccagtgc cggacgcggt cgtgccggca attcggccaa cagcgactcg tgcgcggtca
    29641 gcgccagccg gtgttgcgcc agcgcctggt cgggatggcc gccggcccag cggcagtcgg
    29701 ccgcggtcag gtgcacggcg atggtctgcg ctgccggcat cggggtaacg aatcgccacg
    29761 cgctggcggc gtccgcataa gcgttcgcca gcgctgggtc gtcgacgtag gcccgcgccc
    29821 gagcgagcag cgaccagacc acagcagcgg cgctcaggtg atcgaccagc gggagtcggc
    29881 ccagctggtt gcgccacgcg tccagcccgt caagcgtgcg ggcggcggcc tccgccagcc
    29941 cgaggaaccc ctggacggca gcgagctgtg ccgacgcagt gaagaagcgg acgccgtccg
    30001 gtagcgaggt gtacggaccg gacgcgccgc cctgggccag gatgcgcgcc gcctgcacga
    30061 cggcgtccgg tgcgtccgcg gccgcctcgg agaaggccca cgcctggagt ctggtcgcgt
    30121 tcagggcgac catcgcgtcc gccagttccg ccgtccgcga cgggtccgag gtgaccgcga
    30181 cgcggagccg cttcaccgtg gacgcaagct cgcgccgcga atcctcgttg gctggcactg
    30241 tcggcatcgg ccacctcctt ctggtcaagc atgctccaca ccgccgcccc ccgttcgcgg
    30301 aaaggtgctc gcgactggtc catacgggca cacttggcac accatgagtg aactgatcga
    30361 gcaaccccgg ctcacgattc gccggatttc ggtgtcgagt atggacaaca acgtctatct
    30421 actcaccacc cgcacgggcg gtcgccagtt gttgatcgac gctgcccacg agccggacgc
    30481 gatcgcggaa ctactcgcgt ccgctgcggg ggacgcggac gtaccgccgc ggctcgaact
    30541 gatcctgacc acgcactccc accacgacca cctcggggcg ttggccgcgg tcgcgtccgc
    30601 tcaccccgag gcgcagacgc ttgccggggc agcggacgcc ggcgcgatca cggcagccac
    30661 cggcgtcccg atcgcgcggc gtctggaaca cggcgacgcc atcgcgctcg gcgacctgga
    30721 actgcaagtg atcggtttgc gcggacacac gcccggctcg gtcgcggtgg cgtacagcga
    30781 gcccgaccag cagacgcacg tgttcaccgg cgactcgctg ttccccggcg gcgtgggcaa
    30841 caccaaccgg gatgcggagc ggttcaacag cctgttctcg gacgtggtcc agcgcatctt
    30901 cgaggtctac cgcggagact cgatcgtgca ccccggccac ggcgcggcga ccacgctctc
    30961 cagcgagcgt ccgcacctgg ccgagtggga agctcgcggc tggtgagttg gtccccgacg
    31021 cgtgaacggc gaggctcagc tgcgccggaa tagccgctgc accaggtgcc aggcggtgcc
    31081 gggcggatcg tcggtcgtga acgcccactc cgggtcggtc agcatgcggg caccggacag
    31141 tccggccacc atggcgatta ccgcgagcac tgtcgcgacg ccctgctgca gcgcggacac
    31201 aacgtcggcc tgatcgaagt agatcagcgc ccgcagggcg gaggcgccgg ggatcgcgac
    31261 cagcaccgtg ggcacggtca tgatgatctt gttgaggtcg aacagccgag cggcgagcgc
    31321 gcacagcagg cccatcacga agcagcacac gaaggtggcg atgtgggcct tcacgccgtt
    31381 ctgcagcagc aggaggcgcg ggatgttgcc cagcagcgcc accaccccgg acatgaccgc
    31441 ggccgacagc ggcgagttga acatcgtcgc ccaaccgaag accgcgaaga agctggcggc
    31501 cagttgcacc ggccagagca gcgccggctg gatcgcgagt ggcgggacgg cgtccggtga
    31561 aaccccggtg ggggtgacca ccagccatac cccgatcgtg atcgacagca gcaccatcgc
    31621 ggcgtagaac aaccgcggca ctccggccag caggtcgatt cgggtgaggt cgagaccggc
    31681 ggtgaccagc gggaaaccgg gaatcaggaa gatggccgca cagatcaatc cggcggccat
    31741 gcgcgggctg gggctgccga gggcgagatc gaggcccctg gtaaacacgg cataaaggcc
    31801 ggtggccgtg gcggcggagg tgagcacgac ggcgagatgg ttgagttgca ggccggtcag
    31861 cgtccggtgc aaccagtagg cgagtgcgga cgccggcagc accgccagca cctcgcgcca
    31921 gccgccgttg ccgaggatgg tgaccgagcc gcacgccagc gcgaccagca ggatcagcgc
    31981 ccacgccgga tagagccgcg gggtgtgctc gatgtcgtcg agcatcgcgt caacttcggc
    32041 gacggtggcc cgctcgggaa ggttttggcc gaggcgctgc atcaggtcga tgcggtgggc
    32101 gttgacgccc ggcttgcgga cctcgcccac ctgcgtgcgg aaagtgccgc gtcgctgcac
    32161 ggtcagcacg atgtcggtga acgtgacctg tgctctgatg ttgccgatgc cgaccgtccg
    32221 ggcgaccgcc tgcatcactt cgcgtacccg cagcccggag gtgccggcgc cgagcatcag
    32281 gatgccggcc cgcagcacca cgttcgctcg ccggaccagg tgcttgggct ccagggcgtc
    32341 ctcatggaag tgctcgtcga aggtcggttc ccgggtgtca gccacgtgcg ccaagcctac
    32401 gcgggttgat gggcggtctc cgacgttcga acggtggacg ggatgcgacc ggctcagcct
    32461 ggatccaggc aaggcccgcg aaagcggccc cgagaacgtc ggggtgctgc tcggaccggg
    32521 cctgaacctc aagcgccatc cggccggcgg acgcaacttc gagtacttct ccgaagaccc
    32581 gctgctctcc ggcaaggcgg ccgccgcgct ggtgcgtggc atccagtccg agggtggaag
    32641 ccggtgaatt caaagtgcgc gtgggcgcgt cctcgcggga catccgcgcc cgcgcgtccg
    32701 tccatgtcga gttggccgac cggatcacgc ccgtgcccaa gcccgctggg aaggtcgaga
    32761 ctgatcctag gggcctagcg gaacggataa gccacgtagg cgaaccgcgt cccgtccggc
    32821 gaccaactgt tgacgttgat ggtgccctga ccgccgggta gctcggcgac ggtgaccgcg
    32881 tccgcccagg cgtcgcggac gaccagccgc agttgcacgg tcaggtcggc cgggtgtccg
    32941 agagtcccgg cagggaagct caggtagacc cagtgccggc cgcccggggc ggtgtgcggg
    33001 aaccagttca cgcgctcatc gaaggtgagc tgggtgaact cggttccgtc cgggcgcacg
    33061 cgcgcgatct gtgcatgccc gggagtcgtg gagaaccgct cggtgttgaa gtacagccat
    33121 tccccgtcga ctgagtactc ggggccgtcg tccgggcccc agccgggcag cacctcgcga
    33181 tcatccgatc cgtcgacgcc aacggtgtgg aggttgacgc agccccagcc ctgggtcgga
    33241 tcgatgttga cataggcgag cgtggtgccg tccgggctga ctccgtgcag gaagtgcatc
    33301 aggctgccgc gatcgcgagt cacgcgccgg gtctcgccgc cggccaacgg cgccacgtag
    33361 aggtgccggt cgttgccgga taggtagatg ttctcgccgt ccggggccag tacgtgatcg
    33421 ttgttcagct caggggcgcc cactagtgga atctcggtcg ggggagcgga gccgtcggcg
    33481 ggcaatgtcc agagcaggcc gtcgccgttc aggatgagcc ggtcgtcagg tgtccagttg
    33541 ggtgcctcga acagccgctc ggtgtccacg tacaccaccg aatcggtcgc cgtccgcagg
    33601 tcgtaggtgt gtacctcgca gacctgccct gctgcgagtt cgcgtccggc catctgtcgc
    33661 cacctttcga tcaggcatcc attctcgcag cgtccgcccc tccgcaagct ggacagttgc
    33721 agccgacatc ggctccagat gtccagtttc tgagcgaggt gatcagctgg tcgacgagtg
    33781 gcgcgtccgg acggatcacg gttcggtgac ccggcacaaa atgcactgtc gatcaccaga
    33841 atccgcagta atcagcgaaa ccagcgatcg acagtgcaaa ttgtcccgtt cagcccagcc
    33901 ggggtgactg ccgatcggcg cggaacagtt cgcacgccac gtcgttgtag cggtcggtgc
    33961 ggccgaggtg ggtcatgccc aatcgtcggc acaccgcttg cgaagccaga ttctcgggat
    34021 aggtgactgc atagaccgta gtcagcccat gtttccaggc gcgatcaagc agtcgttgcc
    34081 cagcttcggc ggcatacccg tttccccagg cctcggagtg caggtgccag cccacttcag
    34141 tgtcggccga tgcctcgccc gacccgctca gggggagcag cttcatcagc gccgtcccga
    34201 tcgtttgcga ctagcctgac cgcacgatcg cccaaactcc gtgcagcgcg ccgtcgaatg
    34261 ctgtccacgc agccacccgc tcctcggcct gagatcggtc ggccagtacc gccggcgtcc
    34321 ggccgaggaa ccggacgacc tcccagcggg ctcgcatgat cttgcgcaac gccgtgccca
    34381 ccgggtcgtc ggcggacgcg tcgtggctct ccaggactgc catcaccacc ccaagggcct
    34441 cggtggcgct gaacatcagg ggtggcagcc gaattccgcg tccgacggtg tagccgccgc
    34501 caggaccggg cacggcgcgg atcggaattt cggcttggcg caggatcgcc acgtagcgtc
    34561 gggcggcccg ctcggttacc gccagcgcgt cggccagctc ggccgcggtg atgcctggct
    34621 ggttctgcag cagttcgagg gcgcgcaggg ctcgggcggt ggggctggtc t
//
LOCUS       DAOPRQ010000023        32844 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_1050_length_32844_cov_11.601757, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000023 DAOPRQ010000000
VERSION     DAOPRQ010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 32844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32844
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1050_length_32844_cov_11.601757"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            168..602
                     /gene="arfB"
                     /locus_tag="PKJ61_04545"
     CDS             168..602
                     /gene="arfB"
                     /locus_tag="PKJ61_04545"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008363515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HOC13126.1"
                     /translation="MRIPLAPGLPGGLVIPSAELVERYSHASGPGGQGVNTSDSRVQL
                     SFDVEASGALDETQRARLLHRLRPRLVGTTVTVVGAEFRSQRQNRAAARERLAALLID
                     ALAPPSPPRRATRPTRGSIDRRLTSKRLRSQLKRSRTRPLDE"
     gene            634..>1670
                     /locus_tag="PKJ61_04550"
     CDS             634..>1670
                     /locus_tag="PKJ61_04550"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOC13127.1"
                     /translation="MDQSAGTPTFDAIVIGAGPNGLVAANALADAGWDVLVLEAQAEA
                     GGAVRSGTVTADGFTTDLFSAFYPLAAASPLIRDLELDRHGLEWVQAPNVLAHQLDDG
                     RAAVLHRDPERTAAGLDEFAPGDGDAWLELYGQWERIRDGLLSALFTPFPPVMAGTRM
                     LRALRVAGALRLARLGVTPVRRLGEELFRGDGGRLLLTGNAMHSDVPPDGAGSGMFGW
                     LLAMLGQDVGFPVPKGGAGRLAGALRARAESLGAEVRTSSPVTEVLVRSRRAHAVRLA
                     DDTMISARRAILADVTAPALYGDLLDSVTLPSRVREDLTKFHWDHATLKLNWALDAGV
                     PWLAEESRQAGT"
     assembly_gap    1671..1770
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1889..2374)
                     /locus_tag="PKJ61_04555"
     CDS             complement(1889..2374)
                     /locus_tag="PKJ61_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017932924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HOC13128.1"
                     /translation="MTGSSEAGHRKDPGPTISRRVAAPADAVWSVLADGWSYANWVVG
                     AARVRDVDSGWPAKGTRVHHSFGLWLLLIQDFTRVESVTPQRELVLTARGWPVGEAHV
                     HISVRPDGAEASEVTITEDAVSGPGKLIPPPIRHLLLGPRNRETLHRLALLAEGHFRR
                     D"
     gene            complement(2538..2831)
                     /locus_tag="PKJ61_04560"
     CDS             complement(2538..2831)
                     /locus_tag="PKJ61_04560"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOC13129.1"
                     /translation="MTRRRVEPANESPRVVLITGASSGIGRAVAQQLAGRGYRLVLCS
                     RSDVALAAVADECRQLGASDATVLVVPTDVRDAAAVDRLFEAAVDRFGRVDAA"
     gene            2937..4004
                     /locus_tag="PKJ61_04565"
     CDS             2937..4004
                     /locus_tag="PKJ61_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13130.1"
                     /translation="MRKWCAVFVAMFALGGCSAVAPTTPATAPPRGGITPQLLALLAE
                     EHLGSAAAVTGQSGPVVAVRPTEWVDGLSATAYYRATPGQPAGWLTVSYSPMPYAGAD
                     CTGATCTERDGVQVRSDPDEVIARRDPGFVQVEASAAYFEGELLEAAIALAADPRIAP
                     TVDGNLLNKAAANPRWRDTDDLGCGDASLQAPIALPEPDDSSQPATPQALAAVVASQV
                     DASCAGDESATNLVRGTVYLGADTERVSLAINTEPIQCPYLDACEQQGDLIVARQLDV
                     PTDYPVRIVLARPLPDGNHWVVVEHASLRAQPSGNFPVPLSTLKSLVSDARVGAQVDA
                     ALNRAGDELPLRWRLTPRTSE"
     gene            4036..4782
                     /locus_tag="PKJ61_04570"
     CDS             4036..4782
                     /locus_tag="PKJ61_04570"
                     /inference="COORDINATES: protein motif:HMM:NF039283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5911 domain-containing protein"
                     /protein_id="HOC13131.1"
                     /translation="MSRQIEDYALVSDCRTGALISRDGSVDWLCLPRYDSASTFGALL
                     GDPDHGRWLVAPTDPDAAASRRYLDRTFVLVTTWRTATGELEVTDFMPHGGGRSDLVR
                     RLRCLHGEVEVHEDLRVRFGYASALPWVRQLTEDAKPVLIAVAGPDAVVRRGPRLTAE
                     GHAHTGDYRLRARGHRARHGRGRCPLPRSGRATFADRGRALPHEPGDGGHRRLSARAC
                     RVSPHALGVTRPALGRLSRGWPAPDPARSC"
     gene            complement(4739..6184)
                     /locus_tag="PKJ61_04575"
     CDS             complement(4739..6184)
                     /locus_tag="PKJ61_04575"
                     /inference="COORDINATES: protein motif:HMM:NF025670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13132.1"
                     /translation="MAQHPRIYPTGAALLSLSLLLACMPASVAAATTALPSVAGKCPG
                     EAARDIGDIDGDGVADPVVETFGPTGGGPSYLDVHNSTAPEQVIGGADVGIPAVASLG
                     GTTLVVDVNRDGCADVVTNGNDGIYLIPGSGAGLVPSAAVRLARPEGEDDWGSSFAVM
                     KDLRLLVVGAPGSTASPHGGGAVYLYPLTNDGTPGTAAILHQDSPGIPGNSEKGDAFG
                     STIAVDGKLLVIGAPGESDRKKRSGAVTLLKFTGVGLAFNAVRWTQDSSGFETRAASD
                     EQMGDQLSVRANYIAIGVPEERFGSGYAVGIVHVVALANSQKPKVKKVYNFHQNSPGI
                     PGKNENTDRWGSAVALAANVGCTPMSVVIGAQGDKVGKTPSGAVTVVGLGKRCASRWL
                     PKATFGLSFRYFGREIVTLSSAAGGKAPDVVLVGDAFSRAIGDDVFETGQVVAVTTTG
                     KGKWKAAAVPPRDGYHAFSNYGRGLGLANHG"
     gene            6271..6528
                     /locus_tag="PKJ61_04580"
     CDS             6271..6528
                     /locus_tag="PKJ61_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HOC13133.1"
                     /translation="MSEAVIHSARGVVKFWRDEKGWGVISSEALPPGRDAWAHFSVID
                     ADGFRSLAQGQAVDFRYHATKQDSFDFVADWVRAVDEPDFG"
     gene            6613..7506
                     /locus_tag="PKJ61_04585"
     CDS             6613..7506
                     /locus_tag="PKJ61_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13134.1"
                     /translation="MGATRGAAQRPRAPWSDRTRVLVIAVCLLTVAGLALTAWSLRAG
                     WGVVPATPAAAETKVMIDVFSGRENPVVTLDAAVAEELYLMLADHESAGEVHAGAAPD
                     AVLGFRGLVVTPADDSRPTLRILPDTFHFERDDSWWRIDDTGQSFYDRVYHAIRPLVS
                     ADVRKLLPDSNPPIPEVSAVVPPAQGVTATWRLADPAEVSADSVSLSLLVTRLECSGG
                     KTGELMEPVVSVSAEDIVIRVDAVPNPAGTYTCPGNDEVAVAVTLTEPVGVRPLVDAA
                     CLAGDAVRTSYCADGAVRWRP"
     gene            complement(7653..8897)
                     /locus_tag="PKJ61_04590"
     CDS             complement(7653..8897)
                     /locus_tag="PKJ61_04590"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOC13135.1"
                     /translation="MTDARGVERLYLLLTLLTTLAASFIWGVNTLFLLDAGLSNTEAF
                     LANAFYTLGMVLFEVPTGVVADTRGRRFSFLLGTGTLLASTLLYLWMWDLRAPMWGWA
                     ISSVLIGLGFTFFSGATEAWLVDALAATGFKGSLDAVFGRAQVVAGGAMLTGSVLGGV
                     IAQFTDLGMPYLVRAGVLALTFAVGWWAMRDLGFTRQAHASPVAAVRHVLAGAVAGGL
                     RNRPVRWVMLAAPFTAGTGVYVFYALQPHLLQLYGDPNAYGVAGLAAALSAGAQIVGG
                     LLAPRLRRLFRLRTSVLMVGGLLGVVLLGLLGLVSNFVVALALVAIWSMAGAAVAPLR
                     QSFVNGAIPSAQRATVLSFDSLLGSAGGVVAQPALGRVADAAGYPASFLASAGVQVLA
                     LPFILLARRERAASDPIVAVDR"
     assembly_gap    9061..9160
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(9224..11410)
                     /gene="glgX"
                     /locus_tag="PKJ61_04595"
     CDS             complement(9224..11410)
                     /gene="glgX"
                     /locus_tag="PKJ61_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017933056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HOC13136.1"
                     /translation="MQVWPGSPYPLGATYDGRGTNFALFSEVADRVQLCLFDGDRTES
                     RLDLTEVDAHVWHGYLPQVDPGQRYGYRVHGPHDPANGLRCNPNKLLLDPYAKATDGQ
                     IEWHQSLFGYNFGDENSRNDEDSAERMMLGVVVNPFFDWTGDRRLNVPYNESIIYEAH
                     VKGLTALHPDVPEAERGTYAGLVQPAVLKHLQSLWVTAVELMPVHQFVHDATLVEKGL
                     RNYWGYNTLGFFAPHAEYSAIRDGGGQVQEFKGMVKTLHEAGIEVILDVVYNHTAEGN
                     HLGPTLSFRGIDNPAYYRLVDDRRQYYLDYTGTGNSLNVGNPHSLQLIMDSLRYWVLE
                     MHVDGFRFDLAAALAREFYDVDRLATFFELVQQDPVISQVKLIAEPWDVGPGGYQVGN
                     FPPLWTEWNGKFRDSVRDFWRGEPNLGEFAARIAGSADLYEQSGRRPFASINFVTAHD
                     GFTLRDLVSYNDKHNEVNGEDNRDGESFNRSWNHGVEGATDDPEVLAERARAQRNFLS
                     TLLLSQGVPMLLHGDESGRTQNGVNNTYAQDSDISWVHWDALDAPLMEFTATIAKLRA
                     EHPTFRRKRFFTGRASHVGEEQRLNDIVWLHPDGRPMADADWTADGARVIGMFLNGDA
                     IAGKDGTGNAIVDDSFLLYFNGGADDVELTLPPAEYAPEWDVAIDTAADGPEAAPLAA
                     GAARTLAGSSALVLIEHANYDAEPEMSAAASVAAMIKIEDEETNGG"
     gene            complement(11451..11960)
                     /locus_tag="PKJ61_04600"
     CDS             complement(11451..11960)
                     /locus_tag="PKJ61_04600"
                     /inference="COORDINATES: protein motif:HMM:NF017758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HOC13137.1"
                     /translation="MADKGLEDLFHDTLRDVYYAERHILKTLPKLEASATLDELKAAF
                     NKHTAETEGQIQRLESVFGLIDRKPSAKKCDGIEGILTEGDHVLSEYEGTSALDAGLI
                     SSAQAVEHYEITRYGTLRRWAKMLALPDAYDLLSETLEEESRTDELLTKIADAEANAK
                     AKAAKAKSK"
     gene            complement(12032..13312)
                     /locus_tag="PKJ61_04605"
     CDS             complement(12032..13312)
                     /locus_tag="PKJ61_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008355047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOC13138.1"
                     /translation="MPRSRSPLLTSLLGLRGNGRACVYTEPMWGLSMMLVLPYASVFM
                     LALGVHDQQIGLLATISVLSQVVFGLLSGVITDKLGRRATTAIFDIIAWSIPCLIWAF
                     AQNFWWFLVASVINGAWQVTQNSWDCLLVEDVDRGEITHVYSLIKVAAEFSALFAPIA
                     AVLVAEFGLEPAVRILYLNAFVIMSAKIYILWRWSTETEVGRQRQQQTRGVSIWRLLA
                     EYRGVLGLILKSKGTIFSLAISALVGAVALVNGTFWQVVINQHLLVPDPLLPFFPMIR
                     SVLSVVFFFTVIPWLTHQKHLRLPTLLGFLVYLAGQVLLVVIPAPDGAANWSTYAMLG
                     VCLLLDAFGASILFMLAESLVALHVDRDERSRVMAIQRTCVMLVTAPFGWISGWLSGM
                     DRTWPFILTSLLLVVGVVVTSRFWVTTHSEPVTV"
     gene            complement(13376..14833)
                     /locus_tag="PKJ61_04610"
     CDS             complement(13376..14833)
                     /locus_tag="PKJ61_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HOC13139.1"
                     /translation="MSTNTAATVEPEVVDRLPATSVRVLAVLLVGAFVVILNETALNV
                     ALSRIMTDLSIDERTAQWLTTGFMLTMAVVIPITGWLMERFATRTVFTLAMSLFSVGT
                     LVAALGWAFPSLLAGRIIQASGTAIMMPLLMTTVMELVPAKLRGRVMGTISLVISAAP
                     AIGPTLSGVILQFLPWRFVFVLVLPIALAILLLGLRQVPNLNTPVAVKLDVLSIPLTG
                     FGFGGLVYGLSLVGSPTAGWLLEAALGVGVVSLGLFIWRQLVLQRTDAALLDLRTFTY
                     PIFTTALGVMAIAMAVLFGTIIALPLILQQVLHAEPLYVGLLLLPGALLTGLLGPVVG
                     RLYDRVGPRWLIVPGAVAVSVALGLFGQLAVTTQWWQLLIAHLTLSLGLAFMFTPLFT
                     ITLGALPPHLYGHGSAMLGTVQQVAGAAGTALFVTVMAGVAAAQPETLGAEQSLAAGA
                     RAAFLTVGAIWLVGFLATLFLRKPEDSEAGAPPVH"
     gene            15024..15983
                     /locus_tag="PKJ61_04615"
     CDS             15024..15983
                     /locus_tag="PKJ61_04615"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOC13140.1"
                     /translation="MIRLATIGTSSITRTLATAVAQTDGIQIDVVYSRDAERAAALAI
                     DLRAPESSSDLTAMLAREDLDAVYIASPNSLHAQHASAAIGAGKHVLLEKPAAPSATE
                     WAELVGHAQAAGVVLLEAMRTAYDPGLQAVRQLLPELGRLRRVSFRYEQRSSRYANVL
                     AGERVNIFDPAMAGGALYDLGVYCIHPLISLFGEPSRVVGESVLLDTGADGAGVALAV
                     YPDFIADLSYSKITSSELPSEIQGEDATLTIDQIASPRLLRLARRDGQVTEHQVTGPP
                     QHGLHGEVAHFVRLIEAGESAARDQTLTLQTLRVIDQLRAARV"
     gene            16546..17127
                     /gene="idi"
                     /locus_tag="PKJ61_04620"
     CDS             16546..17127
                     /gene="idi"
                     /locus_tag="PKJ61_04620"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002516279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HOC13141.1"
                     /translation="MTDRDRRAITTATSADADLVVLLDAHGRPAGTAPRAEVHGFDTP
                     LHLAFSSHLFDDSGRVLLTRRALTKRTWPGVWTNSCCGHPRPGEHAADAIRRRVADEL
                     GAEVTGLRLVLPEFRYRATDDSGVVENEICPVWVGQLRGTLRPNPAEVMATTWAEWDE
                     VVTLAKATPALLSPWAVQQVPLLGIELRKAGAA"
     gene            17124..18251
                     /locus_tag="PKJ61_04625"
     CDS             17124..18251
                     /locus_tag="PKJ61_04625"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOC13142.1"
                     /translation="MSLATHPDRSHRRLESSPAESDNATVAAVDRLIEGTLATLREHW
                     TDLAAGVVPRPGVLHTDSVLELLAGLAMSGGKHLRPRMVHWGWVAASGRVSGGYEDLV
                     RAGAALELLHVFALIHDDVMDQSETRRGRPSVHAEARRQHLRLRGLGDSERYGENIAI
                     LAGDLAHSEADALVAGLPERMQQLWRTLSTELMIGQGRDLVGAANGRRDLEHAREVAR
                     AKSGAYTVWRPLQLGAAAAGAAEATFTALERYGIAVGEAFALRDDILGVVGDAKATGK
                     PVGDDLLAGKPTVLLALATQRFSPRWRRVLARAGTANVRAGDVAALRGELVRSGVIDE
                     VEGMIAAAVEAATHALDDPAIAPEAAVGLHALARRVAWRTA"
     gene            18248..19867
                     /gene="crtI"
                     /locus_tag="PKJ61_04630"
     CDS             18248..19867
                     /gene="crtI"
                     /locus_tag="PKJ61_04630"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HOC13143.1"
                     /translation="MSRVIVVGAGLAGLASACRLVGDGHEVTLIERATDPGGRNGIIR
                     EGGFTFDSGPTVFTMPHLLDRAFGHLGRRLADEVRLARLDPAYRAVYADGTQLRVRAE
                     LGDQLAEIRTVVSERDAAAFTDFVKWLEELYAVEMPNFIERNFDSPLDLLTRPGAAAK
                     LIGLGGFGRLGKAVGQRFADDRLVKLFTFQAMYAGLAPADALAIYAVITYMDSVAGVW
                     FPEGGMHAVPAALASALGESGAELRFGTSVTSILRDSSGRVAGVVAGGEQLRADAVVV
                     TLDLPVAYRELLSDLTPPKVLAGAQYSPSAVVWHVGVRGLPGAGVAHHNIHFGSEWTR
                     SFEQLIRRKELMSDPSRLVTIPSLDAPDMAPDGHSALYVLEPVPHLGARIDWTRESGP
                     MRERLHRFLGDHGYPDDVVVERLVTPADWAAQGMAMGSPFSLAHTFFQTGPFRPRNFE
                     PRVPGVFFAGSGTTPGVGVPMVLISGELAGVRVRQYLNAASASSQRPPSSRLTSSSSR
                     PTSSSSRPTSSSSRRRPGSQSPITTASQAER"
     gene            19879..20772
                     /locus_tag="PKJ61_04635"
     CDS             19879..20772
                     /locus_tag="PKJ61_04635"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HOC13144.1"
                     /translation="MTELAAGYAECARLTREYGTTYYWGALLLPKARRRHVYAIYALC
                     RLADDVVDAPGATTDRTPETRAALAGFQQRFEAALAGSPDDAVMAAIAASVTECGIDP
                     ECFDRFFSAMAMDLDVDRYENWADLCAYMEGSAAVIGEMMLPVLQPASAEAREPARAL
                     GFAFQLTNFLRDVNEDLDRGRVYLPQADLARFGASPWHRLVDEPWRRTMALLIARNRE
                     LYAQARTGIPLLPSRSARCVATALTLYSQILDRIERNDYDVFSGRLRVPTWRKAATAL
                     TILATGPRLAKAPALGGQLAS"
     gene            20762..21118
                     /locus_tag="PKJ61_04640"
     CDS             20762..21118
                     /locus_tag="PKJ61_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase domain-containing protein"
                     /protein_id="HOC13145.1"
                     /translation="MRAEYLLLLAACLVITLPLEFVLGARVYRRPRRLTLAVLPVLVV
                     FYLWDAIAIARGHWDYHPGRTSGVILPGAMPLEELAFFIVIPLCGLLTYEAVGNVLRN
                     SRARRTRARAVGERHA"
     gene            21111..21425
                     /locus_tag="PKJ61_04645"
     CDS             21111..21425
                     /locus_tag="PKJ61_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase domain-containing protein"
                     /protein_id="HOC13146.1"
                     /translation="MPEYTIATLVAVVVVVVVELRWARTGIFATAQYWLAMAIVFAFQ
                     VIVDGWLTRLPDAIVRYAPQHFLGVRFPLNIPVEDFGFGFALVTATIIAWKVAERREP
                     VS"
     gene            21422..22948
                     /locus_tag="PKJ61_04650"
     CDS             21422..22948
                     /locus_tag="PKJ61_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012921467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOC13147.1"
                     /translation="MRAWRPGRDPKAVRHGPSTSADGSLAVADTQPQVLVVGGGIAGL
                     AAATGLAERGVRVTVLEAEGTLGGRVRAWPVAAPGGGTTTMSRGFHAFFRQYYNLRSL
                     LRRADPALSRLLPIEDYPLQLAPSTSRPAPVRDSFRNVPRTPPWNLVGFVAQSPSFGL
                     RDLARVDVREALGLLQVRFPQTYTDLDGISAAEVLDRLHFPDSARHLALEVFARSFFA
                     DPREFSGGELIAMFHTYFVGSAEGLLFDVPDDDYDTALWAPLGRYLAALGVNVATGSR
                     VRGLEHSGAGIVAHSDAGEVHADAVVLALDTRGLQQVVAESPWLGDAAWRQRIEGLRI
                     APPFGVGRRWLSGRFPDVASFLGTSAYGPLDNLSRVDAFESGAARWAARSGGTVVEAH
                     GYALTDTSDATRDRLWAEAARLHPELGAAKVLAEEWLVEADCALVDTSPWRLRPEVTT
                     PDPRVVLAGDLVRCDWPIALMERAATTGWQAANALLARLGKPGHELWSPPLRGLLARS
                     "
     gene            complement(22964..24175)
                     /locus_tag="PKJ61_04655"
     CDS             complement(22964..24175)
                     /locus_tag="PKJ61_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HOC13148.1"
                     /translation="MSAAPAPQQRRAHRPGETDAPRVERDGSVWRIRSLETARQVLRA
                     RNLTTQAGFTSEAIPKGYLKHHPILISDGPSHDEQRRKVGRFFAPAVVAERYTGLMES
                     CADALLAEAAASGKFRLDDLALYYAVEVTAEVVGLTDSPVRRMSRRLVSFFNQPPFDI
                     TRPDLGRTPRQWAQAAVNGLGPVLRFYLSDVRPAIRERRKRPRQDVISHLIAEDYTNA
                     DILVECVTYGTAGMVTTREFIAMAAWHLLTNDPLRERYLVAGQPERVAILNEIIRLEP
                     VVGHLYRRAQGEFEIADGDQTWTVAAGDLLDLSVRTANTDPAAVGEAPLGLCPERPLP
                     SGVNPAVLSFGDGAHKCPGQPLAILESDVFLTRLLALKPTIVTEPELGWDDLIAGYWL
                     RNFQLSFPATI"
     gene            complement(24172..25077)
                     /locus_tag="PKJ61_04660"
     CDS             complement(24172..25077)
                     /locus_tag="PKJ61_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOC13149.1"
                     /translation="MDGTDARGQVRDFLVSRRARITPERAGLPTWGGNRRVAGLRREE
                     VALLAGVSVEYYVRLERGNLAGVSESVLEAIATALQLDDAERSHLHDLARTVGTKGRK
                     PRVLPRDRLRPPVQWLLDAMTGSAAYVRNARLDLLAANRLGAALYAPIFEMAGRPNVA
                     RFIFLDLRGREFFVEWDRVAAESTALLRTLAGENPYDRGLSELIGELSTRSDEFARLW
                     AEHNVRQHRTGTKRFRHPIVGELTLAYESMDLTTDPGLRLNAYVAEPGSPSAEAFGLL
                     ASWATTPVVSLARIEDRATGDGGLN"
     gene            complement(25090..26103)
                     /locus_tag="PKJ61_04665"
     CDS             complement(25090..26103)
                     /locus_tag="PKJ61_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HOC13150.1"
                     /translation="MYGAVIHAPGDVRYEERAEPTIIEPTDAVVRTVAACVCGSDLWR
                     YRGISPVPEPTPIGHEFCGIVEVVGDAVRSVKPGDFVVGGFNPSDNTCPVCRKGATAN
                     CQHGSHYDGCQAERIRIPHADGTLLATPGQPDDDLIPSILALSDVMCTGWHAAVSAGV
                     GPDTTVAVVGDGAVGLSAVLAAVQLGAPTVIAMSRHADRQRVATEFGATHIVAARGAD
                     GAAKVKELTDGIGADAVLECVGTEDSRLQATECVRDGGNIGLVGVPHGALPMDSLFWR
                     NVGVKGGPAHVRAHLPKLLELVLDRKIDPGLVFDLELPLSEVAEAYRAMDERRAIKAL
                     LRP"
     gene            complement(26202..27287)
                     /locus_tag="PKJ61_04670"
     CDS             complement(26202..27287)
                     /locus_tag="PKJ61_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008358519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HOC13151.1"
                     /translation="MTVLHATEPATVHLSLWARVAEVKVADVEAALYNSRELVKQLAM
                     RRTLFVFPRELLPAAWGSASARVAGTEFRRLVADIERAGVAEDGAAWVETAAVAVLER
                     LAGGNEADALALRNEVPLLQSRIVFGGGKWSTDAPVAPRVLTLLGARGELVRGPNGGH
                     WRISRPRWTTMSTWLRQSDDELQRDTESDGYAELVRRWLWTFGPGTETDLAWWLGATK
                     TAVRRALADVGAEQVGLDSGDLGWVLPGDTEAEEVRPWAALLPVLDATTMGWKQRGFY
                     LDADDVPYLFDSAGNGGTTAWWNGRIVGCWVQTPDGSVQVVLRHDPGRLARAALNAEA
                     ERLTAWLGGVVVSSVYSSQQMRGARLP"
     gene            complement(27386..29149)
                     /locus_tag="PKJ61_04675"
     CDS             complement(27386..29149)
                     /locus_tag="PKJ61_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011758024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOC13152.1"
                     /translation="MSHDIAEAPADSTNQTTFPDLGLGDRLLRTLRDVGYETPSPIQA
                     ETIPALLDGRDVVGLAQTGTGKTAAFALPILDRLDLKQKAPQALVLAPTRELALQVCE
                     AFQKYAARLTGVHVLPVYGGQGYGVQLSALARGVHVVVGTPGRIMDHLEKGTLDLTQL
                     RFLVLDEADEMLNMGFAEDVETILADTPSDKQVALFSATMPGPIRKLSKKYLNDPVEV
                     RVVGKTQTAANVRHRYVVVSYPQKVEALTRILEVENFEAMIVFVRTKNETETLAEKLQ
                     ARGFSAAAINGDVAQAQRERTIGHLKSGKLDVLVATDVAARGLDVERISHVVNFDIPL
                     DTESYVHRVGRTGRAGRSGDAISFVTPRERRLLGAIEKATRQTLTPVPMPTAEDVNAT
                     RLSRFDDAITSALDSERLDFFREVVAHYVNEYDVPEVDVAAALALQLQGEEPMLIDPK
                     REPVAPDARSERGGKDRRDRYDSDDRPERGGRPQREARGSEAPMATYRLAVGKRHRVE
                     PRQIVGALANEGGLRRADFGHIDIRPDHSLVELPAHLPQHVWDALRTTRISGKLIELS
                     PVQPRRRPDDGNKPYRKGRRS"
     gene            29237..30016
                     /locus_tag="PKJ61_04680"
     CDS             29237..30016
                     /locus_tag="PKJ61_04680"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HOC13153.1"
                     /translation="MDAIATESERFIAAIEQTEPADRVPTCPEWTADDLLWHLTEVHG
                     YWAGVLHRNAQTEAEAEEVEANAPERAEDREQIIELFNANTAALIGELEQRSDAEPAW
                     FWLESAKTVGSTRRMQAHETTMHRVDAELAAGLESVGVDPDLAADGIAHAFDVMWAWW
                     GTLPGFAFVEVGGPVELVASDLGRSWLAQPGRWQGTGQSGKHYDQSGAVLRPAGEPVA
                     HYTGTVEQLYRWLWGRGPEPDASGDAVSLDGLRAAQAQGMQ"
     gene            30013..30636
                     /locus_tag="PKJ61_04685"
     CDS             30013..30636
                     /locus_tag="PKJ61_04685"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOC13154.1"
                     /translation="MTATEPSEFWEARYAGQPQVWSGRVNRVFAEVVGQFAPGRALDL
                     GCGEGGDAVWLAEHGWTVTAVDISATAVERGRGAAVERGVADRIEWVAADLGSWRTDA
                     RFDLVTASFLQSPVSLPRGQILRAAAELVAPGGHILIVAHAAPPPWASQLHGHHREFL
                     TAAEQLAELGLDETWRVELAETRSRQATGPDGVEATLDDSVVLATRS"
     gene            30633..31496
                     /locus_tag="PKJ61_04690"
     CDS             30633..31496
                     /locus_tag="PKJ61_04690"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOC13155.1"
                     /translation="MTSRRALLTGAGAVLAAHLTGCQSAGAKTPPSAATSPPGRPVMP
                     ATPSRSSRPLRFVVGSDGHLGDPKAPGDPYPAFVKTVNALHAQASLDFVVVNGDITQG
                     GVKRQQQVKGVLAGLKMPYFVVPGNHDELTRAQWRKVWRAEPNLVERFGDRSIILANT
                     SNSAGDYLCAKRSWLQEALHDQASQQDVLVFMHITPDDWTKFGVDCPNIRKVLSQTPN
                     LRAVFNGHDHDQDGQMTSAGVDYFFDAHLGGHWGTDYVGFRLSSLDGNSLTTQMVKLD
                     GTLLPETSLSW"
     gene            31520..32548
                     /locus_tag="PKJ61_04695"
     CDS             31520..32548
                     /locus_tag="PKJ61_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012918889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HOC13156.1"
                     /translation="MQAVVYDRPGATPVLTEVPAPVCPAHGAVIAVRATGVCRSDWHA
                     WKGHDPVPLPNIPGHEFAGVVAQVGSAVHNFSVGDRVTVPFVNGCGQCGYCRKGQAQV
                     CPTQTQPGFTHPGSFGELVAVRAADFNLVRLPESVGFVAAAALGCRFATSFHALVAQA
                     ELGAGEWLVVFGCGGVGLSAIQVARALGARVVAVDPSAAARELAEQLGAEATLAVSEP
                     AAIADLTGGGAHVSMDAVGSAATSGAAVRSLRRRGRHVQVGLMLAADADAGLPWGELV
                     AKELRVIGAHGMAAADYPAMLAMIADGRLDPTALIGRIVGLPEAGAALMAMDGPAVGA
                     GLTVVELA"
BASE COUNT         5110 a        11199 c        11216 g         5119 t
ORIGIN      
        1 gccgccagcc cggcaagcac caaagcccgc cgccggctga gccggaacgc gtccaatccg
       61 ttcgccattt gagcattctc tggcaagccg tccggtcccg cagcccggat agctcacttg
      121 ccacgacaac gcagcgccgg acgacctacc gttgcctggt gagggagttg cgcataccgc
      181 tggcaccggg attgcccggc gggttggtga tcccgtccgc cgaactcgtg gagcggtact
      241 cgcacgcatc cgggccaggc ggccaagggg taaacacgtc cgactcccgc gtccagttgt
      301 ccttcgatgt cgaggcctcc ggtgccctcg acgagaccca acgggcacgc ctgctgcatc
      361 gcttacggcc acggctggtc ggcaccacgg tcacggtcgt cggggccgag tttcgttcgc
      421 agcgccagaa ccgtgcggcc gcaagggaac gcctcgcagc gctcctcatc gacgccctcg
      481 cgccaccttc gccgccgcgg cgcgccaccc gtccgacccg tggttcgatc gatcggcggc
      541 tcaccagcaa gcgcctgcgc agccaactca agcgcagccg gacgcggccg ctggacgaat
      601 agcaatgcgc tgacaccggg taaggaggac gtcatggacc aatctgctgg cacgcccacc
      661 ttcgacgcga tcgtgatcgg agccggcccc aacgggctgg tggccgccaa cgccctcgcc
      721 gacgccggtt gggacgtgct cgtcctcgag gctcaggccg aagcgggcgg cgcggtgcgc
      781 agcggcaccg tcaccgcgga cggcttcacc accgacctgt tcagcgcgtt ctacccgttg
      841 gcggccgcga gcccgctgat ccgcgacctc gaactggacc ggcacggcct cgaatgggtg
      901 caggcgccca acgtgctcgc ccaccagctc gacgacggcc gtgcggccgt cctgcaccgc
      961 gatccggaac gcaccgccgc ggggttggac gagttcgcac ccggtgacgg cgatgcgtgg
     1021 ctggagttgt acgggcagtg ggagcgcatc cgcgacggcc tgctcagcgc gctgttcaca
     1081 cccttccctc cggtcatggc cggcacccga atgctgcgtg cgctgcgggt cgccggggca
     1141 cttcgactgg cccgacttgg cgtgacccca gtgcgtcggc tgggcgagga gttgttccgc
     1201 ggcgacggcg gacggctgct gctcaccggc aacgccatgc actccgacgt gcccccggac
     1261 ggcgcgggca gcgggatgtt cggctggctg ctggcgatgc tcggccagga cgtgggcttc
     1321 cccgtcccca agggtggcgc ggggcgcctg gccggtgcct tgcgcgcacg ggctgaatcg
     1381 ctcggcgccg aggtgcggac gagctcaccg gtgaccgagg tgctggtgcg gtcgcggcgt
     1441 gcccacgccg tccggctggc cgacgacacc atgatctccg ctcgtcgcgc gatcctggct
     1501 gacgtcaccg ccccggcgct ctacggcgac ctgctcgatt cagtgacgct gccgtcccgg
     1561 gtacgcgaag acctgaccaa gttccactgg gaccacgcca cgctgaagct caactgggcg
     1621 ctcgatgctg gcgtgccctg gctggccgag gagtcccggc aggcgggcac nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gccggggcga taagggcgag ccggggcgat
     1801 aaaggcgggc cgggatggcg aagtcgcgca acggagctct ccgtcatccc ggcgcaggcc
     1861 gggatctcgt caacgggcga acgctcagct agtcgcggcg gaagtgaccc tcggcgagca
     1921 gagcgaggcg gtgcagcgtc tcccgattcc gggggccgag cagcaggtgc cggatcggcg
     1981 gcgggatcag cttgccgggc ccggataccg cgtcctcggt gatggtcacc tcgctggctt
     2041 cggcgccgtc cggacggacg ctgatgtgca cgtgcgcctc gcccaccggc cagccgcggg
     2101 cggtcagcac cagctcgcgt tgcggcgtca cgctctcgac tcgggtgaag tcctggatga
     2161 gcagcagcca cagtccgaac gagtggtgca cccgggtgcc cttcgccggc cagcccgaat
     2221 cgacatcgcg gacgcgcgcc gcgcccacca cccagttggc gtacgaccag ccgtccgcca
     2281 acaccgacca gaccgcgtcc gccggagcgg ccacccgacg cgagattgtt gggcctgggt
     2341 ctttccgatg cccagcttcg gagcttccgg tcatcgagct tccttcctct ggttggcttc
     2401 caaagcgtcc gtacccacca ctgaccgccg cgcccgcggg aaccgcctgc atcacattcc
     2461 ccgcagtcgt cgggatgtcg gctcgacggc cctgtgccag cgcggaatag aggggtccta
     2521 cgagcagatc gaaaagctca ggcagcgtcc acgcgtccga atcggtcgac cgccgcttcg
     2581 aacagccggt cgaccgcagc cgcgtcccgg acatcggtag gcaccaccag gacggtcgca
     2641 tcggaagcgc cgagctgtcg gcattcgtcc gccaccgcag ccagggccac atccgagcga
     2701 gagcagagca ccagccggta gccgcgtccg gccagctgct gagcgaccgc tcggccgatc
     2761 ccgctgctgg cgccggtgat cagcaccacg cgcggagatt cattcgctgg ttccacccga
     2821 cgcctggtca tgcactgctg ttaccccgca gctcccgcct aggttacgtt cgtctcagcc
     2881 tggtctgcgg caaagctgca taagctgagc tttcgcggtg ctggacggag gtatgcgtgc
     2941 gcaagtggtg cgcggtgttc gtggcgatgt tcgccctggg tggctgctcg gctgtggctc
     3001 cgaccacccc cgcaaccgcg ccgccacgtg gtgggatcac cccgcaactg ctggccctgc
     3061 tggccgagga acacttggga agcgcggccg cggtgaccgg gcagtccggg ccggtcgtgg
     3121 cggttcgtcc caccgaatgg gtggacggac tcagtgctac cgcctactac cgcgcgaccc
     3181 caggacaacc ggccggctgg ctcacggtca gctattcgcc gatgccgtat gccggcgccg
     3241 attgcactgg cgcgacgtgt accgagcggg acggcgtcca agtgcgaagc gatcctgacg
     3301 aggtcatcgc caggcgcgac cctgggttcg tgcaggtcga ggcctcggcg gcctacttcg
     3361 aaggcgagtt gctggaggcc gcgatcgcgc tcgccgcaga cccccggatc gcacctaccg
     3421 ttgatgggaa tctgctcaac aaggccgcag ccaacccgcg ctggcgggac accgacgacc
     3481 tgggctgcgg ggacgcgtcg ctacaggctc cgattgccct ccctgaaccg gacgactcca
     3541 gccagccggc gaccccgcag gcactggccg cagtggtcgc tagccaggtg gacgccagtt
     3601 gcgcgggcga cgaaagcgcc acgaacctcg tccgcggcac ggtgtatctc ggcgcggaca
     3661 ccgagcgggt gagcctggcc atcaacaccg agcccatcca atgcccgtat ctggacgcct
     3721 gcgaacagca gggcgacctg atcgtcgccc gccagctgga tgtgcccacc gactatcccg
     3781 tccggatcgt cctggcccgg ccgctgccgg acggcaacca ctgggtggtg gttgagcacg
     3841 ccagcctccg cgcgcagccg agcgggaact tcccggtgcc gctgtccacg ctcaagtccc
     3901 tggtcagcga tgcccgcgtc ggcgcccagg tggacgcggc cctcaaccgc gccggcgacg
     3961 aactgccgtt gcgttggcgg ctcacgccac gcacgtccga gtagccgagc cggcaccttg
     4021 gggtaactgc ccagggtgag ccggcagatc gaggactacg cgctggtcag cgactgccgc
     4081 accggcgcgc tgatctcgcg cgacggcagc gtcgactggc tgtgcctgcc gcgctacgac
     4141 tcggcttcga ccttcggcgc gctcctcggt gaccccgatc acggccgctg gctggtcgcc
     4201 cccactgatc cggacgcagc cgcgtcccgg cgctacctgg accgcacctt tgtgctggtc
     4261 acgacctggc ggacggctac cggcgagctg gaggtcaccg acttcatgcc gcacggcggc
     4321 ggccgctccg atctggtccg ccggctgcgc tgcctgcacg gtgaggtcga ggtgcacgag
     4381 gatctgcggg tccgcttcgg gtacgcgtcc gccctgccgt gggtgcgcca gctcaccgag
     4441 gacgccaagc cagtgctgat cgcggtggcc gggccggacg cggtcgtccg acgcgggcca
     4501 cggctaacgg ccgaaggtca cgcgcacacc ggcgactacc ggctgcgggc gcgaggacac
     4561 cgagcacgcc atggtcgcgg gcgatgtcct ctacctcgct ccgggcgcgc gacattcgct
     4621 gatcgcggac gcgccctgcc acatgagcct ggtgatggtg gacaccgacg cctgagcgct
     4681 agggcatgtc gcgtaagccc gcacgcgctc ggggttacgc gacccgccct gggccggctc
     4741 agccgtggtt ggccagcccc agaccccgcc cgtagttgct gaacgcgtgg tagccgtccc
     4801 gcggtggcac ggcggcagcc ttccatttgc ccttgccagt ggtggtcacc gcaacgacct
     4861 gtccggtctc gaagacgtcg tcgccaatcg cccgggagaa cgcgtcgccc accagcacca
     4921 cgtccggggc cttcccgccg gctgccgaac tcaaggtgac gatctcgcgt ccgaagtagc
     4981 ggaaggacaa cccgaaggtc gccttcggca gccagcgcga cgcgcaccgc ttgcccaggc
     5041 cgaccacggt caccgcacct gaaggcgtct tgccgacctt gtcgccctgc gcaccgatca
     5101 cgacgctcat cggcgtgcaa ccaacgttgg ctgccagggc caccgcgctg ccccagcggt
     5161 cggtgttctc gttcttgccc ggaatcccgg ggctgttctg gtggaagttg tagaccttct
     5221 tcaccttcgg cttctggctg ttggccagcg caaccacatg cacgatgccg accgcgtaac
     5281 cgctaccgaa ccgctcctcg gggacaccga tcgcgatgta gttcgcgcgc acgctcagtt
     5341 ggtcgcccat ctgctcatcg ctcgcggcgc gggtttcgaa accggagctg tcctgcgtcc
     5401 agcgcaccgc gttgaaggcc agaccgaccc cggtgaactt cagcaaggtg accgcacccg
     5461 agcgcttctt gcggtccgac tcaccgggcg caccgatcac caggagcttc ccgtccacag
     5521 cgatcgtcga gccgaatgcg tcgccctttt cactgttgcc cgggatgcct gggctgtcct
     5581 ggtgcaggat ggccgccgta ccgggcgtac cgtcgttggt cagtggatag aggtacacag
     5641 ccccgccgcc atgcggtgaa gctgtggagc ctggcgctcc gaccacgagc agccggagat
     5701 ccttcatcac cgcgaaggac gagccccagt catcctcacc ctcgggcctg gccaagcgga
     5761 cggctgcgct cggcaccagc cctgcccccg accccggaat caagtagatg ccgtcattgc
     5821 cgttggtcac cacgtccgcg cagccgtccc ggttcacgtc caccaccagc gtcgtgccgc
     5881 ccagcgacgc gaccgctgga atgcccacgt ccgcgccgcc gatcacctgc tcgggggcgg
     5941 tcgagttgtg cacgtccagg taggacggac cgccgcccgt cggtccgaag gtttccacga
     6001 ccggatcggc cacaccgtcg ccgtcgatgt cgccgatgtc gcgagcggcc tcgcctggac
     6061 acttgccggc gacggacgga agcgctgtcg tcgcagccgc cacagatgcg ggcatgcagg
     6121 cgagcagcag cgacaaactc agcagtgcgg cgccggttgg gtagatcctg gggtgttgag
     6181 ccatgtcacc acggtaactc cgttcgaccc ggacgcaggt gtttcgcccc ggcagtcggc
     6241 tgatctgccc ggaggctagg gtgcatgtac atgagcgaag cagtgatcca ctcagcccgc
     6301 ggcgtggtca agttctggcg ggatgagaag ggctggggcg tgatcagcag tgaagcgctg
     6361 ccgccgggac gggacgcctg ggcccacttc tcggtgatcg acgcggacgg cttccgctcg
     6421 ctcgcccaag gccaggcggt cgactttcgc tatcacgcaa cgaagcagga cagcttcgac
     6481 ttcgtcgccg actgggttcg cgccgtcgac gagcctgact tcggctgatc aaccatggct
     6541 gcaacggacc cgcgcgtccg gggcgtgtgc gtagtgtgag tatgcacgtc gagccggccg
     6601 aaggaggctg cgatgggagc gacaaggggc gctgcacagc gaccgcgggc accgtggagt
     6661 gaccgcacgc gcgtcctcgt catcgcggtg tgcctgctga cggtcgccgg cctcgcgctg
     6721 acggcgtggt cccttcgcgc cggctggggc gtcgtcccgg ccactccggc agcagccgag
     6781 accaaggtga tgatcgacgt cttctcgggt cgggagaatc cggtggtgac cctcgacgct
     6841 gcggtcgccg aagagctcta cctgatgctc gccgaccacg agagcgcagg cgaggtgcac
     6901 gccggggctg ccccggatgc ggtcctcggc ttccgcggcc tggtggtgac accggcggac
     6961 gactcgcgtc cgacgctgcg gatcctgccg gacacgttcc acttcgagcg cgacgacagc
     7021 tggtggcgca tcgacgatac ggggcagtct ttctatgacc gggtctacca cgcgatccgt
     7081 ccgctcgtct ccgcggacgt gcgcaagcta ctgccggaca gcaacccgcc gatcccggaa
     7141 gtgagcgccg tcgtaccgcc tgcgcagggg gtgacggcga cctggcgact cgcggatccc
     7201 gccgaggtga gcgccgactc ggtgagcctg tcactgctgg tgacccggct ggagtgttcc
     7261 ggtggcaaga ccggggagct gatggaaccc gtggtctcgg tgagcgcgga ggacattgtg
     7321 atccgcgtcg acgcggtccc gaaccccgcc ggcacctaca cctgcccggg caacgatgaa
     7381 gtagcggttg ccgtcaccct gaccgagccg gtgggcgtcc gaccgctcgt cgacgccgcc
     7441 tgcttggctg gggacgcggt gcgcaccagc tactgcgcgg acggggccgt ccgctggcgg
     7501 ccctgaggaa ccgctgatca acgttctcca acggtggctg aggtggtcca acggtggctg
     7561 agcctgacga agcccgtagt caacgcgcgg atggacgacc cttcggcaag ctcagggatc
     7621 gttgatcagc gtctccccac gcgtccgtct gatcagcgat cgacagccac gatcgggtcg
     7681 gacgcagccc gctcgcgccg ggcgagcaga atgaacggca gtgccagcac ttggacgccg
     7741 gcgctggcca gaaaggatgc ggggtagccc gccgcgtccg ccacccggcc cagtgccggc
     7801 tgcgcgacga caccgccggc cgagcccagc agcgagtcga acgacagcac ggtcgcccgc
     7861 tgggcggacg gaatcgcgcc gtttacgaac gattggcgca gcggcgccac cgcggctccg
     7921 gccatcgacc agatcgccac cagcgcgagc gcgaccacga aattgctgac cagaccgagc
     7981 aaccccagca gcaccacccc gagcagaccg ccgaccatca ggacgctggt gcgcagccgg
     8041 aacagtcgcc gcagccgcgg tgcgagcagc ccaccgacga tctgcgcacc ggcggacagt
     8101 gcggcggcca gcccggcgac cccgtacgca ttcgggtcgc cgtacaactg cagcaggtgc
     8161 ggctggagcg cgtagaagac atagacgccg gttccggcgg tgaaaggcgc ggccagcatc
     8221 acccaccgca ccggacggtt acgcaggcca cccgccaccg caccagccaa cacgtgccgc
     8281 accgcggcca ccgggctggc atgggcctgg cgagtgaacc cgaggtcgcg catcgcccac
     8341 cagcccactg cgaaagtgag cgcgagcacg cccgcccgaa ccaggtacgg catgcccaga
     8401 tcggtgaact gggcgatcac cccacccagc accgagccgg tgagcatggc gccgcccgca
     8461 accacctggg cgcggccgaa gaccgcatcg agactgccct tgaaaccggt cgcggcaagg
     8521 gcgtccacga gccaagcctc agtggcgcca gagaagaacg tgaatcccag cccgatcagc
     8581 actgaggaga tcgcccagcc ccacatcggt gcccggagat cccacatcca caggtacaac
     8641 agggtggacg cgagcagcgt gcccgtgccg agcaggaatg agaagcgacg cccgcgagtg
     8701 tcggccacca ccccggtcgg cacctcgaac aacaccatgc cgagggtgta gaacgcgttc
     8761 gccaggaagg cttcggtgtt gctcaggccg gcgtccagca ggaagagcgt gttgacgccc
     8821 cagatgaagg acgcagccaa ggtggtgagc agcgtcagca gcaagtagag ccgctccacg
     8881 ccgcgggcat cggtcaccgc gcaaggctag ctcaagggtc gagtcagctg gggctcggca
     8941 ggctcgacca gcgggggctt cgtcaagctc agccaccgtt ggggttcagg cttcgtcagg
     9001 ctcggccagc gggggcttcg tcaagctcag ccaccgtttc ggctcaggct tcgtcaagct
     9061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ggcttcgtca agctcagcca
     9181 ccgtttcggc tcaggcttcg tcaagcttag ccaccgttcg ggctcaacca ccgtttgtct
     9241 cctcgtcctc gatcttgatc atcgcggcta cggacgcggc ggcggacatc tccggttcgg
     9301 cgtcgtagtt ggcgtgctcg atcagcacca gggcgctgct gcccgcgagg gtgcgggcgg
     9361 cgccggccgc caggggtgcg gcctcgggac cgtccgctgc ggtatcgatc gcgacgtccc
     9421 actccggcgc gtactcggcg ggcggcaggg tcaattcgac atcgtcggca ccgccgttga
     9481 agtacagcag gaagctgtcg tcgacgatcg cgttgccggt accgtccttg ccggcgatcg
     9541 catcgccgtt caggaacatg ccgatcaccc gggcgccgtc ggcggtccag tccgcatccg
     9601 ccatcggacg gccgtcaggg tgcagccaca cgatgtcgtt cagccgctgc tcctcgccga
     9661 cgtgacttgc tcgtccggtg aagaagcgct tgcgccggaa ggtggggtgc tcggcccgca
     9721 gcttcgcgat cgtcgcggtg aactccatca acggcgcgtc caacgcgtcc caatgcaccc
     9781 aggagatgtc ggaatcctgg gcataggtgt tgttcacccc gttctgggtg cggccgctct
     9841 cgtcgccgtg cagcagcatc ggcacgccct ggctcaacag cagcgtgctc aggaagttgc
     9901 gctgcgcccg cgcgcgctcg gccagcacct cgggatcgtc cgtggcacct tccaccccgt
     9961 ggttccagga gcgattgaag ctctcgccgt cgcggttgtc ctcgccgttc acctcgttgt
    10021 gcttgtcgtt gtacgacacc aggtcgcgca gcgtgaagcc gtcgtgagcg gtgacgaagt
    10081 tgatgctcgc gaacgggcgg cgtccggatt gctcgtagag gtcggcggag ccggcgatcc
    10141 gggcggcgaa ctcgcccagg ttcggctcgc cccgccagaa gtcgcggacg gaatcgcgga
    10201 acttgccgtt ccactccgtc cacagcggcg ggaagttgcc gacctggtag ccgccggggc
    10261 cgacgtccca cggctcggcg atcagcttca cctggctgat caccggatct tgctgcacga
    10321 gttcaaagaa ggtggccagc cggtccacgt cgtagaactc ccgggccagc gccgcggcca
    10381 ggtcgaagcg gaaaccgtcc acgtgcatct ccagcaccca gtagcgcagc gagtccatga
    10441 tcagctgcag cgaatgcggg ttgccgacgt tgagggagtt gccggtgccg gtgtagtcga
    10501 ggtagtactg ccgccggtcg tccaccagcc ggtagtaggc cgggttgtcg atgccgcgga
    10561 agctcagcgt cgggccgaga tggttgccct ccgcggtgtg gttgtagacc acgtcgagga
    10621 tcacctcgat gccggcttcg tgcagggtct tgaccatccc cttgaactcc tgcacctggc
    10681 cgccgccgtc cctgatggcc gagtattcgg cgtgcggcgc gaagaagccg agggtgttgt
    10741 agccccagta gttgcgcagc cccttctcca ccagggtggc gtcgtgcacg aactggtgga
    10801 cgggcatcag ttcgaccgcg gtcacccaga gcgattgcag gtgcttgagc acggccggct
    10861 gcaccaggcc ggcgtaggtg ccgcgctcgg cctcgggcac gtcggggtgc agcgcggtga
    10921 gtcccttcac gtgcgcctcg tagatgatgc tctcgttgta gggcacgttg agccggcggt
    10981 cgccggtcca gtcgaagaac gggttgacca ccacacccag catcatgcgt tcggcggagt
    11041 cctcgtcgtt gcgcgagttc tcgtcgccga agttgtagcc gaacaacgac tggtgccatt
    11101 cgatctgccc gtccgtggcc ttggcgtacg ggtcgagcag cagcttgttc ggattgcagc
    11161 gcagcccgtt cgccggatcg tgcggaccgt ggacgcggta gccgtaccgc tggcccgggt
    11221 ccacctgggg caggtagccg tgccagacgt gcgcgtccac ctcggtgagg tcgagccggc
    11281 tctcggttcg gtcgccgtcg aagaggcaga gctgcactcg gtcggccact tcgctgaaca
    11341 gcgcgaagtt ggttcctctg ccgtcatacg tcgcgcccag tgggtagggc gatccgggcc
    11401 agacctgcaa tccatgctcc ttggtcgaaa tggttggtgg gttgcgccta ctacttggac
    11461 ttggccttgg ccgccttcgc cttggcgttg gcttccgcgt ccgcgatctt cgtgagtagt
    11521 tcgtcggtgc gcgactcctc ctcaagagtc tcgctcaaca ggtcgtaggc gtccggcaac
    11581 gcgagcatct tcgcccagcg gcgcagggtg ccgtaccggg tgatctcgta gtgctccacg
    11641 gcctgcgcgg acgagatcag ccccgcgtcc aatgcgctgg tgccctcgta ctcggagaga
    11701 acgtggtcgc cctcggtgag gatgccctcg attccgtcgc acttcttcgc cgacggtttg
    11761 cgatcgatca ggccgaacac cgactccagt cgctggatct ggccctcggt ctcagcggtg
    11821 tgcttgttga aggctgcctt cagttcgtcc agggtggcgg acgcttcaag cttgggcagt
    11881 gtcttcagga tgtgccgctc ggcgtagtag acgtcgcgca gggtgtcgtg gaacaggtct
    11941 tcgaggccct tgtcggccat ggtggtcctc ctcggtgtag ttggtcaccg gttctgtacc
    12001 catggcatcc gtagctaacc cctgcgcagc ctcagacggt caccggctcg ctgtgcgtgg
    12061 tcacccagaa ccgcgaggtc acgacgaccc cgaccaccag gagcagtgag gtcaggatga
    12121 agggccaggt gcggtccatg cccgacagcc acccggagat ccagccgaac ggagcggtga
    12181 ccagcatcac gcaggtgcgc tggatcgcca tcactcggga acgctcatca cggtcgacgt
    12241 gcaacgcaac cagcgattcg gcgagcatga acaggatgct cgcgccgaac gcgtccagca
    12301 gcaggcagac cccgagcatc gcgtaggtgg accagttcgc ggccccgtcc ggcgccggaa
    12361 tgaccaccag cagcacctgg ccggccaggt acaccaggaa ccccaggagc gtgggcaggc
    12421 gcaggtgctt ctggtgcgtc agccagggga tcacggtgaa gaagaacacc accgagagca
    12481 ccgagcggat catcgggaag aacggcagca gcgggtcggg caccaacagg tgctggttga
    12541 tcaccacctg ccagaaagtg ccgttgacca gggcgacggc ccccacgagc gccgagatcg
    12601 ccagcgagaa gatggtgccc ttcgacttga ggatgagccc gagcacgccc cggtactcgg
    12661 ccagcagccg ccagatgctc actccgcggg tctgctgctg tcgctgccgt ccgacctcgg
    12721 tctcggtcga ccagcgccag aggatgtaga tcttcgcgct catgatcacg aacgcgttca
    12781 gatacaggat gcggacggcc ggctccagcc cgaactccgc taccaggacg gcggcgatcg
    12841 gcgcgaacag cgcggagaac tcggcggcca ccttgatcag cgagtagacg tgggtgatct
    12901 caccgcgatc gacgtcttcg accagcaggc agtcccagga gttctgggtg acctgccagg
    12961 cgccattgat gacggacgcg accaggaacc accagaagtt ctgtgcgaac gcccagatca
    13021 ggcaggggat cgaccacgcg atgatgtcga agatggcggt ggtggcccgg cggccgagct
    13081 tgtcggtgat cacgccgctg agcaggccga acaccacctg ggagagcact gaaatggtcg
    13141 ccagcaaccc gatctgctgg tcgtgcacgc cgagcgccag catgaacacg gacgcgtacg
    13201 gcagcaccag catcatcgaa agtccccaca tgggctcggt gtacacgcag gcacgaccgt
    13261 tgccgcgcag cccgagcagg ctggtcagca atgggcttct cgatctcggc accggatcag
    13321 ttaagcactc gcggtgacac gattcatccg gacgcggagt ggcccgcgac cccgatcagt
    13381 gcacgggcgg ggcacccgct tccgaatcct ccggcttgcg caggaacaag gtggccagga
    13441 agccaaccag ccagatggcg ccaacggtca ggaatgccgc ccgggcgccg gcagcaaggg
    13501 actgctcggc gccgagggtc tccggctggg cagcggccac accggccatc accgtgacga
    13561 agagtgccgt cccggccgca ccggcgacct gctgcaccgt accgagcatc gccgagccat
    13621 ggccatatag atgcggaggc aaggcaccga gggtgatggt gaacaagggc gtgaacatga
    13681 acgccaggcc aaggctgagg gtgaggtggg cgatcaacag ctgccaccac tgcgtggtca
    13741 ccgccagctg gccgaacagt cccagcgcaa cggacactgc cactgcgccc ggcacgatca
    13801 gccaacgcgg cccgacccgg tcgtagaggc gtccgacgac cggtccgagc agaccggtga
    13861 gtagcgcgcc gggcagcagc agcaggccga cgtacagcgg ctcggcatgc agcacctgct
    13921 gcaggatcag cggcagcgcg atgatcgtgc cgaacagcac cgccatggcg atcgccatca
    13981 cccctagcgc cgtggtgaag atcgggtagg tgaaggtgcg caggtcgagc agagcggcgt
    14041 ccgtccgctg caggacgagc tgtcgccaaa tgaacaggcc gagcgagacc acgcccactc
    14101 cgagggcggc ttcgagcagc cagcccgcgg tcgggctgcc gaccagactc agtccgtaca
    14161 ccaggccacc gaacccgaag ccggtcagcg gaatgctgag cacgtcgagc tttaccgcca
    14221 ccggggtgtt caggttcggc acctgccgca aaccgagcag caggatcgcc agtgcgatcg
    14281 gcaacaccag tacgaacacg aaccgccacg gcaggaactg caggatcact cccgaaagcg
    14341 tcggcccgat ggccggcgcc gccgagatca ccaggctgat cgtgcccatc acccggccac
    14401 gaagcttggc cggcaccagc tccatcaccg tggtcatcag caggggcatc atgatcgccg
    14461 taccgctggc ctggatgatt cgtccggcca gcaggctcgg gaacgcccag ccgagcgctg
    14521 caaccagggt gccgacgctg aacagcgaca tggcgagggt gaacaccgtc cgggttgcga
    14581 accgttccat caaccagccg gtgatcggaa tgacgacggc catggtcagc atgaagccgg
    14641 tggtcagcca ttgcgcggtg cgctcatcga tgctcaggtc ggtcatgatc cgcgacagcg
    14701 caacgttcag cgcggtctca ttgaggatca ccacgaacgc gcccaccagg agcacggcga
    14761 gcacgcggac ggaggtcgcc ggcagccggt cgacaacttc gggctcgacg gtagcggcgg
    14821 tgttggtgga catctatcgg tactcccatg cggttcggca gctgaggtcg gcggcgcgcg
    14881 gccgcgggca ggactagccg ttcgggatca aggcgctgtc tgatgggtat attcccgagt
    14941 gaagcccgga cagctcgaca ccggtcgatg aagccagcgt cgtgggcagg ttgggctaag
    15001 caagaatgcg gaagaggaga cccatgatca gactggccac gatcggcacc agttcgatca
    15061 cccggacgct cgcgacggcg gtcgcccaga ccgacggcat ccaaatcgat gtcgtctatt
    15121 cccgggacgc cgagcgtgcg gcagccctcg cgatcgatct gcgtgcaccg gagtcgagct
    15181 cagacctgac cgcgatgctg gcccgcgagg acctggacgc ggtctacatc gccagtccca
    15241 acagcctgca cgcgcagcac gcatcagcag cgatcggggc cggcaagcat gttctgctgg
    15301 agaagccggc ggcgccctcc gctacggagt gggcggaatt ggtcggtcat gcccaggccg
    15361 ccggtgtggt gctcctggag gccatgcgca ccgcgtacga ccccggcctg caggccgtcc
    15421 gccaactcct gcccgagctg ggtcggctgc ggcgggtgtc cttccgctac gagcaacgct
    15481 cgtcgcgcta cgccaacgtc cttgccggcg aacgggtgaa catcttcgat cccgccatgg
    15541 ccggcggtgc gctgtacgac ctcggtgtgt actgcatcca cccgctgatc agcctgttcg
    15601 gggaaccgtc gcgcgtggtc ggtgagtcgg tgctgctgga caccggtgcg gacggggctg
    15661 gcgttgcgct cgcggtgtac ccggacttca tcgccgacct gtcctactcc aagatcacct
    15721 cctcggagct gcccagcgag atccagggcg aggatgccac cctgacgatc gaccagatcg
    15781 ccagcccgag gttgctgcgc ctggcccgtc gcgacggcca ggtcaccgaa caccaggtca
    15841 ccggaccgcc acagcatgga ctacacggcg aggtggcgca cttcgtccgc ttgatcgagg
    15901 ccggggagtc ggccgcgcgc gaccagacgc tgaccctgca gaccctccgg gtgatcgacc
    15961 agctgcgggc cgcgcgggtg tgaacctcag ccaagcctgg atcgaggcta aggggctgag
    16021 gcatctgttg ctcggcgatc attgagcccg cagccaccga ctcgggtggt cgagcttgtc
    16081 gagaccagct cagcgggtga atctgggctg acaccggtag gttcaaccca gttttgaccg
    16141 cttatgaagc ccccaccatt gcccccggaa agagcaaccc aactttgacc ggcaatggag
    16201 ccagaatccg gcccgataac cggtcgaagt tgggttcact gcggcgaggc cacaaggacc
    16261 gtcgccaaat ccggctgaaa gtgggttcat tctcctccgc gcctcctcaa gatcaaagca
    16321 gaccccagca gtacgaccaa ccgggccccc gagcccggcg cctgacccct ctgaactgcg
    16381 attcagcacc gacttcgaca agctcagccg gcgctacctc agattgattc gcgctttgga
    16441 gagtccggat ccggctaaga attatgacta gccggcctag acaagcgttg tccggccgcg
    16501 ggcccgttgg cagactcttg gaaggcgaac tgaacgggag cccggatgac cgatcgagac
    16561 cgacgtgcca tcaccaccgc gacgagcgcg gacgccgacc tggtcgtgct gctggacgcg
    16621 cacggccgcc cagccggcac ggcacctcgg gccgaggtac acggcttcga cactccgttg
    16681 catctagcct tctcaagtca ccttttcgac gactccggac gggtgctcct gacccgccgc
    16741 gccctcacca agcgcacctg gccgggggtg tggacgaact cctgctgcgg gcatccgcga
    16801 ccaggcgagc atgcagccga tgcgatccga cggcgagtgg ccgacgaact gggcgcggag
    16861 gtgaccggcc ttcggctggt gcttcccgag ttccgttacc gggctactga cgacagcggg
    16921 gtggtggaga acgagatctg cccggtgtgg gtaggacaac tgcgcgggac cctgcggccg
    16981 aatccggccg aagtgatggc gaccacctgg gccgaatggg acgaggtggt caccctcgcc
    17041 aaagccacgc cggcgctgct cagcccatgg gcagttcagc aggtgccgct gttgggaatt
    17101 gaactacgaa aggccggtgc cgcatgagcc tcgccactca tcccgaccgt agccatcgcc
    17161 gtctggagag ctcaccagcc gagtcggaca acgcgacggt cgccgccgtc gaccgcctga
    17221 tcgaaggcac actcgcaacg ctccgcgaac actggacgga tctcgccgca ggggtggtgc
    17281 cacgcccagg ggtgctgcat accgactcgg tgctggagtt gctggccgga ctcgccatgt
    17341 ccggcggcaa gcacctgcgc ccacgtatgg tgcattgggg ctgggtagcc gcctccgggc
    17401 gggtgagcgg aggctacgaa gatctcgttc gtgccggtgc cgcgctggag ctgttgcacg
    17461 tgttcgcgct tatccacgac gacgtcatgg accagtcgga gacccgacgc gggcgtccgt
    17521 ccgtgcatgc agaggcgcgt cgccagcatc tccggttgcg cggtcttggc gactccgaac
    17581 ggtacggcga gaacatcgcc atcctcgcgg gcgacctcgc gcacagcgaa gcggacgcgc
    17641 tggtggccgg cctgccggag cggatgcaac agctgtggcg caccctgagc accgagctga
    17701 tgatcggcca gggcagggat ctggtcgggg cggccaacgg acgccgcgac ctcgagcacg
    17761 cccgcgaggt ggcccgggcc aagtcgggtg cttacacggt ctggcgtccg ttgcagctgg
    17821 gcgcggcggc cgccggggcc gctgaggcga ccttcaccgc cctggagcgt tacggcattg
    17881 cggttgggga ggcgttcgcg ctgcgcgacg acatcctcgg ggtggtcggg gacgccaagg
    17941 ccaccggcaa acccgtgggt gacgacctgc tggccggcaa gccgaccgta ctgctggcgc
    18001 tggccacgca gcgattctcg ccgcgctggc gacgggtgct cgcccgagcc ggcaccgcga
    18061 acgttcgggc cggtgacgtc gcggcgctgc gtggcgagtt ggtcaggtcg ggggtgatcg
    18121 acgaggtgga aggcatgatc gctgccgcgg tcgaggcggc cacccacgcc ctggacgatc
    18181 cggcgatcgc tccggaagcc gcagtcgggc tgcatgctct tgcccgccgg gtggcctggc
    18241 ggacggcgtg agccgggtca ttgtggttgg cgccggcctt gccgggttgg cgtcggcgtg
    18301 ccggctggtc ggggacgggc acgaggtcac gctgatcgaa cgcgcgaccg accctggtgg
    18361 gcgcaacggc atcatccggg agggcgggtt caccttcgac tcgggcccga ccgtcttcac
    18421 catgccgcac ctgctggatc gggccttcgg gcatctcggg cgccggctgg ccgacgaggt
    18481 tcggctggcc aggctcgatc ccgcctaccg agcggtgtac gccgacggca cgcagctgcg
    18541 cgtccgggcc gagctcggtg accagctggc cgagatccgc accgtagttt cggagcggga
    18601 tgctgcggcg ttcacggact ttgtgaagtg gctggaggag ctttacgcgg tcgagatgcc
    18661 gaatttcatc gaacgcaact tcgattcgcc gcttgatctg ctgacccggc ccggagccgc
    18721 ggccaagctg atcggcctgg gcggattcgg ccgcttgggc aaggccgtcg gccaacgctt
    18781 cgccgacgac cggctggtga agctgttcac cttccaggcg atgtacgccg ggctggcgcc
    18841 ggcggatgcg ctggcgatct acgcagtgat cacctacatg gattcggtcg ccggtgtgtg
    18901 gttcccagag ggcggcatgc acgcggtgcc cgcggcgctc gccagtgcgc tgggggagtc
    18961 tggtgccgaa ctgcggttcg gcacgtcggt gaccagcatc ctgcgggatt cgtccggacg
    19021 ggtcgcgggc gtggtcgcgg gcggtgagca gctgcgcgcg gacgccgtgg tggtcaccct
    19081 cgatctcccg gtggcgtacc gcgagctgct gtctgacctg acgccaccca aggtgctggc
    19141 cggcgcgcag tactcgccgt ccgcggtggt gtggcacgtc ggcgtccgcg gcctgccggg
    19201 ggctggcgtg gcccaccaca acatccattt cggcagcgaa tggacccggt ccttcgaaca
    19261 gctgatcagg cgcaaggaac tgatgagcga tccgtcccgg ctggtcacta ttccgagcct
    19321 ggacgcgcct gacatggccc cggacggcca cagcgccctc tacgtgctgg agccggtgcc
    19381 gcacctgggc gcccgcatcg actggacgcg ggagagcggg ccaatgcggg agcggctgca
    19441 tcggttcctt ggcgaccacg gctaccccga cgacgtggtg gtcgagcgcc tggtcacgcc
    19501 ggcggactgg gctgcgcaag ggatggcgat gggcagtccg ttcagcctcg cccacacctt
    19561 cttccagacc ggacccttcc gcccgcggaa cttcgagccg cgggtgcccg gcgtcttctt
    19621 cgccggcagc ggcacgactc ccggtgtcgg tgtgccgatg gtgctgatca gcggcgagtt
    19681 ggcgggggtt cgcgtccggc agtacctgaa tgcagcgtca gcgtcatccc agcgtcctcc
    19741 gtcatcccgg ctcacctctt cgtcatcccg gcctacctcg tcgtcatccc ggcccacctc
    19801 ttcgtcatcc cggcgcaggc cgggatctca aagcccgatc accaccgcga gtcaggccga
    19861 gcgatgaggg caagcgacgt gactgaactg gcagccggct acgccgaatg cgcccggctg
    19921 acccgcgagt acggcaccac ctactactgg ggcgcgctgc tgctccccaa ggcgcggcgc
    19981 cgccatgtgt acgcgatcta tgcgctctgc cgactcgccg acgacgtggt ggacgcgccc
    20041 ggcgccacca ccgatcgaac cccggagacc cgcgctgcgc tggcaggctt ccaacaacgc
    20101 ttcgaggccg ccctcgccgg ctcacccgac gacgcggtca tggcggcgat cgccgcgagc
    20161 gtgaccgaat gcgggatcga tcccgagtgt tttgaccgct tcttcagcgc gatggcgatg
    20221 gacctcgacg tcgaccgcta cgagaactgg gccgacctct gcgcctacat ggagggctct
    20281 gcggcggtga tcggcgagat gatgctgccg gtgctgcaac ccgcgtccgc ggaggccagg
    20341 gagccggccc gggcgctcgg cttcgccttc cagctcacca acttcctgcg cgacgtcaac
    20401 gaagacctcg accgtgggcg ggtctacctg ccccaggccg acctggcccg gttcggtgcc
    20461 tcgccgtggc accgcttggt ggacgaaccg tggcggcgca cgatggcgct gctgatcgct
    20521 cgcaaccggg agctctacgc ccaggctcgc acggggattc cgttgctgcc cagccgctcg
    20581 gcgcgctgtg tggccaccgc gctcacgctg tactcgcaga tcctcgaccg gatcgagcgc
    20641 aacgactacg acgtcttctc cggacggctc cgggtgccga cctggcgcaa ggccgccacc
    20701 gccttgacga tcctggccac tggtccacgg ctcgccaagg ccccggcgct tggaggccaa
    20761 cttgcgagct gaatacctac tgctgctggc agcctgcctg gtgatcaccc tgccgctcga
    20821 gttcgtgctc ggcgcgcggg tgtaccggcg gccccggcgg ctcaccctgg cggtgcttcc
    20881 cgtgctggtc gtcttctacc tctgggacgc gatcgccatc gcccgcgggc actgggacta
    20941 ccatcccggg cgcaccagcg gagtgatcct gcccggcgcg atgccgcttg aagaactggc
    21001 cttcttcatc gtgattccgc tgtgcgggct gctcacctac gaagccgtcg gcaacgtgct
    21061 gcgcaactcg cgggcacgac ggacgcgggc gcgagcggtg ggggagcggc atgcctgagt
    21121 acaccatcgc cacgctggtg gcggtcgtcg tggtggtcgt cgtcgaactg cgctgggcgc
    21181 gaaccggcat cttcgccacc gcccagtact ggctggccat ggcgatcgtg ttcgccttcc
    21241 aggtaatcgt ggacggctgg ctgaccaggc tgccggacgc gatcgtccga tacgccccgc
    21301 agcacttcct cggtgtccgg ttcccactaa acattccggt cgaggacttc ggcttcgggt
    21361 tcgccctggt caccgcgacg atcatcgcgt ggaaggtggc agagcgtcgg gagccggttt
    21421 cgtgagggcc tggcgtccgg ggcgagaccc gaaggcagtt cgtcatggcc catcgacttc
    21481 ggcggacgga tcgctggccg tcgccgacac ccaaccgcag gtcctggtgg tcggtggcgg
    21541 catcgccggc ctggccgccg ccaccggact ggccgagcgc ggcgtccgcg tgacggttct
    21601 cgaggccgaa gggactctcg gcggacgcgt ccgcgcctgg cccgtcgccg cacccggcgg
    21661 gggtaccacc acgatgagcc gcggcttcca cgcctttttc cggcagtact acaaccttcg
    21721 ttcgctgctc cgccgggccg acccagcgtt gagccggctg ctgccgatcg aggactaccc
    21781 gctgcaactg gccccgtcga ccagccgacc agcgccggtt cgggactcgt tccggaacgt
    21841 gccacgtacg ccgccgtgga acctggtcgg cttcgtcgcg cagagcccgt cttttgggtt
    21901 gcgggatctg gcccgggtgg acgtccgcga ggcgctgggg ctgctgcagg tgcgcttccc
    21961 gcagacctac accgatctgg acgggatcag tgcggccgaa gtgctcgacc ggctgcactt
    22021 tccggattcg gcgcggcacc tggccctgga ggtgttcgcc cgcagcttct tcgctgaccc
    22081 gcgggagttc tccgggggcg agttgatcgc gatgttccac acctacttcg tcggctcggc
    22141 cgaagggctg ctgttcgacg tgcccgacga cgactacgac accgcgctgt gggctccgct
    22201 cggccgctac ttggccgcac tcggtgtgaa cgtggcgacc gggagtcgag tgcggggtct
    22261 ggagcactcg ggtgcgggca tcgtcgcaca ctcggacgcg ggcgaggtgc acgcggacgc
    22321 ggtcgttctc gcgctggaca cccgcggcct gcagcaagtc gtggcggaat ctccgtggct
    22381 gggcgatgcg gcctggcgcc agcggatcga gggcctgcgg atcgcgccgc cgttcggcgt
    22441 gggccggcgt tggctgtccg gacggttccc ggacgtggcg tccttcctcg gtaccagtgc
    22501 ctacggcccg ctggacaacc tcagccgggt ggacgccttc gagtccggcg cagcccgctg
    22561 ggccgctcgg tccggcggca cggtggtgga ggcgcacggc tacgcgctca ccgacacctc
    22621 ggacgccacc cgtgaccggc tctgggccga ggcggcccgg ctgcacccgg aactcggcgc
    22681 cgccaaggtg ctggcggagg aatggctcgt cgaagccgac tgcgccctgg tcgacacctc
    22741 gccgtggcgg ctgcgtccag aggtgacgac cccggatccg cgcgtggtgc tcgccggtga
    22801 cctggtgcgc tgcgactggc cgatcgccct gatggagcgc gccgccacca ccggctggca
    22861 ggcggctaac gcgctgctcg cccgcctggg gaaacccggc cacgagctgt ggtcgccacc
    22921 gctacgcggc ctgctcgccc gctcttgatc cgccgcctgc gatctaaatc gtcgcgggga
    22981 agctgagctg gaagttgcgc agccagtaac cggcgatcag gtcgtcccag ccgagttcgg
    23041 gctcggtgac gatagtcggc ttcagtgcca gcaggcgggt gaggaagacg tccgattcca
    23101 ggatggccaa aggctggcct gggcacttgt gggcaccgtc gccgaagctg agcacggccg
    23161 ggttcacccc cgacggcagc ggacgctccg ggcagagtcc gagcggcgct tccccgaccg
    23221 cagcggggtc ggtgttggcc gtccgcacgg acaggtcgag cagatcaccc gcggccacgg
    23281 tccaggtctg gtcgccgtcg gcgatctcga actcaccctg cgccctgcgg tagaggtgcc
    23341 cgaccaccgg ttcgagccgg atgatctcgt tcaggattgc gacccgctcg ggctgtccgg
    23401 ccaccaggta gcgctcgcgc agcgggtcgt tcgtcagcag gtgccaggcc gccatggcga
    23461 tgaactcgcg cgtggtcacc atgccggcgg tgccgtaggt gacgcactcc accaggatgt
    23521 cggcgttggt gtagtcctcc gcgatcaggt ggctgatcac gtcctggcgc ggacgcttcc
    23581 gccgttccct gatcgcgggc cgcacgtccg acaggtagaa acgcagcacc ggtccgagcc
    23641 cgttgaccgc ggcctgggcc cactgccggg gcgtgcgacc gagatccgga cgggtgatgt
    23701 cgaacggcgg ctggttgaag aagctcacca agcgacgcga cattcgccgc accggcgagt
    23761 cggtgagtcc gactacttcc gcagtcacct cgaccgcgta gtacaacgcg agatcgtcca
    23821 gccgaaactt cccggaggcg gccgcctcgg ccagcagcgc gtccgcgcac gactccatca
    23881 ggcccgtgta ccgctcggcc acgaccgccg gcgcgaagaa ccggcccact tttctgcgct
    23941 gctcgtcgtg cgacgggccg tccgagatca ggatcgggtg atgcttgagg tagcccttcg
    24001 ggatcgcctc ggacgtgaac cccgcctggg tcgtcaggtt acgggcgcgc agcacctggc
    24061 gcgcggtctc cagagaccgg attcgccaca cactcccgtc ccgctcgacc cgtggcgcgt
    24121 cggtttcacc cggacggtgc gcccgccgct gctgaggtgc cggtgccgcg ctcaattcaa
    24181 gcccccatcg cccgtcgcac ggtcttcgat cctcgccagt gacacgaccg gagtcgtggc
    24241 ccagctggcc agcagtccga acgcttcagc agacgggctg cccggctcgg cgacataggc
    24301 gttcagccgc agccccgggt cggtcgtcag gtccatcgac tcgtaggcga gcgtcagttc
    24361 accaacgatc ggatggcgga agcgcttcgt accggtgcga tgttggcgca cgttgtgctc
    24421 ggcccacagc cgtgcgaact catcgctgcg agtgctgagc tcgccgatca gctcgctcag
    24481 gccgcggtca tagggattct cgccggccag cgtgcgcaac agcgcggtcg actcggccgc
    24541 cacccggtcc cactcgacaa agaactcgcg acctcgcagg tcgaggaaga tgaagcgcgc
    24601 gacgttgggc cgtccggcca tctcgaagat cggtgcgtac agggctgcgc cgagccggtt
    24661 ggccgccagc aggtcgagcc gggcattgcg gacgtaggca gccgaaccgg tcatcgcgtc
    24721 cagtagccac tgcaccggcg gccggaggcg gtcgcgcggc aggacccgcg gtttccgtcc
    24781 cttggtgccc accgtccggg ccaggtcgtg caggtggctc cgttcggcgt cgtcgagctg
    24841 cagggcggtg gcgatggcct ctaggacgct ctccgagaca ccggccaggt tgcctcgttc
    24901 cagccggacg tagtactcga cactcacccc ggcgaggagt gccacctcct cgcggcgcag
    24961 cccggccacc cggcgattgc cgccccaggt ggggaggccg gctcgctccg gggtgattcg
    25021 ggcacgtcgg gacaccagga agtcccgaac ctggccgcgg gcgtctgttc catccatggc
    25081 tgctccggct cagggacgca gcagcgcctt gatggcacgc cgctcgtcca tggcccggta
    25141 ggcctcggcc acctccgaaa gcggtagctc gaggtcgaac accagcccgg gatcgatctt
    25201 gcgatccagc acgagttcga gcagcttcgg caggtgggcg cggacgtggg ccggcccgcc
    25261 tttgacgccg acattgcgcc agaacaacga gtccatcggc agtgcgccgt gcggcacgcc
    25321 gaccagcccg atgttgccgc catcgcggac gcattcggtt gcctgaagcc gcgagtcctc
    25381 ggtgccgacg cattccagga ccgcgtccgc cccgattccg tcggtgagtt ccttcacctt
    25441 ggcggctcca tccgcaccgc gtgcggcgac gatgtgagtg gccccaaatt ccgtcgcgac
    25501 ccgttgccga tcggcgtgtc ggctcatcgc gatgaccgtc ggggcaccga gctggacagc
    25561 ggccagcacc gcgctgagcc cgaccgcgcc gtcaccgacc accgcgacgg tggtgtccgg
    25621 gccgacgccg gcgctgaccg cggcgtgcca gccggtgcac atcacgtccg agagggccag
    25681 gatgctggga atcaggtcgt cgtccggctg gccgggtgtg gcgagcaggg tgccgtccgc
    25741 gtgcgggatg cggatccgtt cggcctggca gccgtcgtaa tgcgacccgt gctggcagtt
    25801 cgcggtggcg cccttgcggc agaccggaca ggtgttgtcg ctgggattga agccgccgac
    25861 cacgaagtct cccggtttga cggacctgac cgcgtcgccg acgacctcga cgatcccgca
    25921 gaactcgtgc ccgatcggcg ttggctcggg cacgggcgag atgccgcggt agcgccacag
    25981 gtccgacccg cacacacagg ccgccaccgt gcggacgact gcgtcggtgg gctcgatgat
    26041 cgttggctcg gcgcgttcct cgtagcgaac gtcgccgggg gcatggatga cagctccgta
    26101 catggcttca gtccaccacg tctgcggccg cgttgggagg tactgtcaag ccctgtctgc
    26161 cgaaccccca ccacggtcct gaatgtgccg gtgaggaacg atcagggcag ccgggcgccg
    26221 cgcatctgct gtgaggagta gaccgaggac accaccacgc cgcccaacca cgcggtgagc
    26281 cgctcggcct cagcgttcag ggcggcacgg gcgagccgtc cggggtcgtg gcgcagcacc
    26341 acctgaacgc tgccgtcggg cgtctgcacc cagcagccga cgatccgtcc attccaccag
    26401 gcggtggtgc cgccgttccc ggccgagtcg aacaggtacg gcacgtcatc cgcgtcaagg
    26461 tagaacccgc gttgcttcca gcccatcgtg gtggcgtcga gcaccggcag cagggccgcc
    26521 cagggccgga cttcctcggc ttcggtgtcg ccgggcagca cccagccgag gtcaccggag
    26581 tccagaccca cctgctcggc accgacatca gccaaggcac gacgcactgc ggtcttcgtc
    26641 gctcccagcc accaggccag atcggtctcg gtgcccggcc cgaacgtcca caaccatcgc
    26701 ctcaccagct ctgcatagcc gtccgactcc gtgtcacgtt gaagttcatc gtcagactga
    26761 cgaagccaag tcgacatggt ggtccaccgt ggccgagaga tccgccagtg accgccgttg
    26821 ggtcctcgca ccaactcgcc acgggcgccg agcaaggtga gcactcgtgg tgcgaccggg
    26881 gcatccgtgc tccacttgcc gccaccgaac acgatccgcg actgcagcag cggtacctcg
    26941 ttgcgcagcg cgagcgcgtc cgcctcgttt ccgccagcga gccgttccag caccgcgacc
    27001 gcagcggtct cgacccaggc cgcgccgtcc tcggccacgc cggcgcgctc gatatcggca
    27061 acgagtcgtc ggaactccgt cccggccacg cgagccgagg cgctgcccca ggcggcgggc
    27121 agcagctccc gtgggaacac gaaaagcgtg cgacgcatcg ccaactgctt cacgagttca
    27181 cgcgagttgt agagcgcggc ctccacatcg gcgaccttca cctcggcaac ccgcgcccac
    27241 aaggagagat gcacggtggc cggttcggtc gcgtgcagca cggtcattgc ccgggtcgcg
    27301 tcgaccggct cggcgaggcg actctcggga tgcagcccgt gccgaaccgc caggcgcgca
    27361 cggcgcttct cgtcggtgaa gtgcttcagc tccggcgacc cttccggtac ggcttgttgc
    27421 cgtcatcggg gcggcggcgg ggctggacgg gggagagctc gatcagcttt cccgagatgc
    27481 gggtcgttcg cagcgcgtcc caaacgtgtt gtggcaggtg cgcggggagt tcgaccagcg
    27541 aatggtcggg ccgaatgtcg atgtggccga aatcagcgcg tcgcaagccg ccctcgttcg
    27601 ccagggcacc gacgatctgg cggggttcca cccgatgccg cttgcccacc gccagccggt
    27661 aggtggccat cggcgcctca ctgccgcggg cctcccgctg gggccgacca ccgcgctccg
    27721 gccggtcgtc gctgtcgtag cggtcgcggc gatcctttcc gccacgctcg gatcgcgcgt
    27781 ccggtgccac cggctcacgc ttcggatcga tcagcatcgg ttcctcaccc tgcagctgca
    27841 gtgcgagcgc ggcagcgacg tccacctctg gcacgtcgta ctcgttcacg tagtgcgcca
    27901 ccacttcgcg gaagaagtcg agtcgctcgg aatcgagggc agaggtgatg gcatcgtcga
    27961 accggctgag ccgggtggcg ttcacgtcct cggcggtcgg catcggcact ggggtgagcg
    28021 tctgccgggt cgccttctca atcgcgccga gcagccgccg ctcgcgcggg gtgacgaacg
    28081 agatggcgtc gccgctacgg ccggcccgtc ccgtccgccc gacgcggtgc acatacgact
    28141 cggtgtcgag cgggatgtcg aagttgacca cgtggctgat ccgctcgacg tccagccctc
    28201 gggccgcaac atcggtggcc accagcacgt ccagcttgcc cgacttcaga tggccgatgg
    28261 ttcgctcgcg ctgcgcctgg gcgacgtcgc cattgatcgc ggcagccgag aatccgcggg
    28321 cctgcagctt ctcggcgagc gtctcggtct cgttcttggt gcggacgaag acgatcatgg
    28381 cctcgaagtt ctcgacttcc aggatgcggg tcagcgcttc gaccttctgc gggtaggaga
    28441 ccactacgta gcggtggcgg acgttggccg cggtctgggt cttgcccacc acgcgcactt
    28501 cgaccgggtc gttcaggtac ttcttgctga gcttccggat cggcccgggc atggtggcgg
    28561 agaacagcgc cacctgcttg tcggacgggg tgtcggcaag gatcgtctcc acgtcctcgg
    28621 cgaagcccat gttgagcatc tcgtcggcct cgtccagcac caggaagcgc aactgggtca
    28681 ggtcgagcgt ccccttctcg aggtggtcca tgatccgtcc gggtgtaccg acaaccacat
    28741 ggacgccgcg ggcgagggcg ctcagctgca cgccgtagcc ctggccgccg tagaccggca
    28801 gtacatgtac gcccgtgagg cgcgctgcgt acttctggaa cgcctcacag acctgcagcg
    28861 ccaactcccg ggtgggggca agcaccagcg cctgcggcgc cttctgcttc aggtcaagcc
    28921 gatcgaggat cgggagcgcg aaggcggcgg tcttgccggt gccggtctgc gcgagcccga
    28981 ccacatcgcg cccgtccagc agcgcgggga tggtctcggc ctgaatgggc gatggagttt
    29041 cgtagccgac gtcccgcagc gtacgcagca gccggtcgcc gaggccgagg tctgggaagg
    29101 tggtctggtt cgtcgaatcg gcgggggctt cagcaatgtc gtggctcacc ccctgaactt
    29161 accgtgaaca ccccggacaa ggctcgtctg cgctgcgccg gacacaccca tgtggtgtga
    29221 ttgccgaatg gacttcatgg acgcaatcgc gaccgaatcg gagcgcttca tcgccgccat
    29281 cgagcagacc gagccagcgg accgggtgcc gacctgcccg gagtggacag cggatgacct
    29341 gctctggcac ctgaccgagg tgcacggcta ctgggccggc gtcctgcacc ggaatgcgca
    29401 gaccgaggcc gaagccgagg aggtggaggc gaacgcaccc gaacgcgcgg aggaccgcga
    29461 acagatcatc gaactcttca atgccaacac cgccgcactg atcggcgaac ttgaacagcg
    29521 cagcgatgcg gagcccgcct ggttctggct ggagtccgcc aagaccgtgg gctcgacccg
    29581 ccggatgcag gcccacgaga cgaccatgca ccgggtggac gccgaactgg ccgccggtct
    29641 ggagtcggtc ggtgtcgatc ccgacctggc cgcggacggc atcgcccacg cgttcgacgt
    29701 gatgtgggct tggtggggca ctctgcccgg tttcgcgttc gtcgaggtgg gcggtccggt
    29761 cgagttggtc gcgtccgacc tcggccggag ctggctggcg cagccggggc gctggcaggg
    29821 taccggccag tccggtaagc actacgacca gtcgggtgcg gtgctacggc cggccggcga
    29881 gccggtcgcg cactacaccg gcacggtcga gcagctctac cgctggttgt ggggacgcgg
    29941 tccagaaccg gacgccagcg gcgatgccgt cagcctcgac ggtttgcggg ctgcgcaggc
    30001 gcagggtatg cagtgaccgc caccgagccg agcgagttct gggaagcgcg gtacgccggg
    30061 cagccccagg tatggtcggg ccgggtcaat cgggtgttcg ctgaagtggt gggacagttc
    30121 gcgcccggac gagccctcga cctgggctgc ggcgagggtg gggacgcggt ctggctggct
    30181 gagcacggct ggacggtgac cgcggtcgac atctccgcca cggccgtcga acgcggtcgg
    30241 ggtgccgcgg tcgagcgcgg cgtagccgac cggatcgagt gggtggccgc cgatctcggc
    30301 tcgtggcgga ccgacgcccg cttcgacctg gtgacggcca gcttcctgca atcgccggtc
    30361 tcgttgccgc gcgggcagat cctgcgcgct gctgccgaac tggtcgcgcc cggcggtcat
    30421 atactgatcg tcgcccatgc cgcaccgccg ccgtgggcga gccagttgca cgggcaccat
    30481 cgtgagttcc tcaccgctgc cgagcagctg gccgaactgg ggctggacga aacctggcga
    30541 gtagagctgg cggagacccg ctcgcgccag gcaaccggtc cggacggagt cgaggcgacc
    30601 cttgacgatt cggtggtact ggcgacccgg tcatgaccag ccggcgcgcg ctgctcaccg
    30661 gtgcgggtgc ggtgctggcc gcgcacctga ccggttgcca gtcggctggc gcgaagacac
    30721 cgcccagcgc ggccaccagc ccacccggaa ggccggtgat gccggcaacg ccgagccgat
    30781 cgagccgtcc gctccggttc gtggttggta gcgacgggca cctcggcgat ccgaaggccc
    30841 cgggcgatcc ctaccctgcg ttcgtgaaga cggtgaatgc gctgcacgcg caagcatccc
    30901 tcgacttcgt ggtggttaac ggtgacatca cccaaggcgg ggtgaagcga cagcagcagg
    30961 tgaaaggcgt cctggccggg ctcaagatgc cctacttcgt ggtgccgggg aatcacgacg
    31021 agctgaccag ggcacagtgg cgcaaggtgt ggcgggccga gcccaacctg gtcgaacggt
    31081 tcggagatcg cagcatcatc ctcgcgaaca cgtccaactc ggccggggac tatctctgcg
    31141 ccaagaggag ctggctgcag gaggcgctcc acgaccaggc gagccagcag gacgtgctgg
    31201 tgttcatgca catcacgccc gacgactgga cgaagttcgg cgtcgactgc cccaacattc
    31261 gcaaagtgct gtcgcagacc cccaacctgc gggcggtgtt caacggacac gaccacgacc
    31321 aggacggcca gatgaccagc gcgggggtgg actacttctt cgacgcccac cttggcgggc
    31381 attggggtac cgattacgtc gggttccggc tgagcagtct ggacggcaac agcctcacta
    31441 cccagatggt gaagctggac ggcacgctgc tccccgagac cagtctcagc tggtagctgt
    31501 tcagcgatag aaacgagcca tgcaagctgt cgtctacgac cgtccgggcg cgaccccggt
    31561 gctcaccgag gtgccggcgc cggtctgccc ggcccacggg gcggtgatcg cggtgcgcgc
    31621 caccggggtg tgccgttcgg actggcatgc atggaaaggc cacgacccgg taccgctgcc
    31681 caacatccca gggcacgagt tcgccggcgt ggtggcgcaa gtgggctccg ccgtgcacaa
    31741 cttcagcgtc ggcgatcggg tgaccgtgcc cttcgtcaac ggctgcggtc aatgtgggta
    31801 ctgccgaaag gggcaggcgc aggtgtgccc gacacagacc cagcccggct tcacccaccc
    31861 cggctcgttc ggggagctgg tggcggtgcg cgcagccgac ttcaatctgg tgcggctgcc
    31921 cgaatcggtc ggcttcgtgg ccgctgcggc gctcggttgc cgcttcgcca cgtccttcca
    31981 cgcactggtc gcacaggcgg agctgggcgc gggggagtgg ctggtcgtct tcggctgcgg
    32041 tggagtgggg ctttcggcca tccaggtggc ccgcgcactc ggtgctcgag tggtggcggt
    32101 ggatccgagc gcggcggccc gcgagctggc cgaacagctc ggcgcggagg ccactttggc
    32161 tgtctcggag cccgcggcga tcgcggacct gaccggcgga ggagcgcacg tcagcatgga
    32221 cgcggtgggc tccgcggcaa cgtcgggtgc tgcggtgcgg agcctgcgtc ggcggggtag
    32281 gcacgtccag gtcgggctga tgctggccgc cgacgcggac gcgggcctgc cctggggcga
    32341 gttggtcgcg aaggagctgc gggtgatcgg cgctcacggc atggccgccg cggattaccc
    32401 ggcgatgctg gcgatgatcg cggacggcag gctcgatccg acggcgttga tcggccggat
    32461 cgtcggcctg cccgaggcgg gcgccgcgct gatggctatg gacgggccag cggtgggcgc
    32521 gggcctgacc gtggtcgagc tggcctgatt cgtcgcgggc tctcgacacc cgaagaccgg
    32581 gcggctaccg acgtgaacgt ccagcgagta ctcatgggga aatcgagttc ggctgcaccg
    32641 ctgaataccg cggtcgtcgt cggcgtgctc acggtgacac tgttggcggc cgggatgaat
    32701 ttcaacggct ccctgattga gtggtcgggt cggcatcatc gagctggggg catccctggt
    32761 cgcactgacc tgtcgggtcg gggcgtgcgg attgatgggc tggcgttcga cgccgttcct
    32821 gtggttcgcg ctgacgtact ggct
//
LOCUS       DAOPRQ010000024        32214 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_1083_length_32214_cov_13.480519, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000024 DAOPRQ010000000
VERSION     DAOPRQ010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 32214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32214
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1083_length_32214_cov_13.480519"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(252..515)
                     /locus_tag="PKJ61_04700"
     CDS             complement(252..515)
                     /locus_tag="PKJ61_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019484296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HOC13157.1"
                     /translation="MQLEESDLVGVLKRLKRAQGQIGGIIRMIEEGRDCKEVVTQLAA
                     VSKALDRAGFAVIAVGLRQCITADPEGTGVDTSSLEKLFLSLA"
     gene            complement(574..951)
                     /gene="trxA"
                     /locus_tag="PKJ61_04705"
     CDS             complement(574..951)
                     /gene="trxA"
                     /locus_tag="PKJ61_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016455308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOC13158.1"
                     /translation="MATTELTAAVFEQTIAENDIVLVDFWAAWCGPCRMFAPVFEAAS
                     EQHPDLVFGKVDTETERDLAAAARIMSIPTLMVFREGILVYAQPGAMPAPALEELISA
                     VRAADMDAVRAELDEQNSEASNA"
     gene            complement(988..2622)
                     /locus_tag="PKJ61_04710"
     CDS             complement(988..2622)
                     /locus_tag="PKJ61_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020575907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOC13159.1"
                     /translation="MTGTGTTEHVSALAPFGNRAFKWLWIGVVISSVGAWAQMVGAQW
                     LFISDPNAATIVALVQTASVLPMLLFGLAAGALADAFDRRRLLLFVQLYSGTVAIILA
                     VLTWLDRMPPLLLLAFTFLIGAGTAMQVPTWQPLITELVPRSQIAQATRLDMVSVNVA
                     RAVGPAIAGLIIAAWGVPLVFLLTAVSALFLIGALLAWRRPDESPRARERFLPALRAG
                     GRYVRHEPVVRLILGRLAVFVIPGTAMWTLLPLIANRQLGLGASGYGLLFAFLGLGAV
                     AAALSLGRVTKHFSGNQVLSWSAVLFAGAFALTMVVPGLFAAVPLLAILGFTWTATIS
                     TLNAELQLYLPGWVRARALAVYLMVFAGVQAVAAPVWGLVAQHVSLSAAVWAAAALLA
                     AGGLAGLAWHFPESSQLDRSPLVYWGDAQVLYSPEPDAGPVQVMVEYVVPSDRVADWR
                     AAMEDLRRSRMRSGAMRWELYRVGERADTYLEIFAVPSWQEHLHQHEQRLTAQDQEIE
                     LRAFGFATNPPIGTHLLPPPDGPDEDIGSTGPAGGK"
     gene            complement(2724..2796)
                     /locus_tag="PKJ61_04715"
     tRNA            complement(2724..2796)
                     /locus_tag="PKJ61_04715"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2761..2763),aa:Thr,seq:cgt)
     gene            complement(2978..4549)
                     /locus_tag="PKJ61_04720"
     CDS             complement(2978..4549)
                     /locus_tag="PKJ61_04720"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOC13160.1"
                     /translation="MPSTIRRLVVIALALALGACTPTPAPPSAPSASVVPENGIRISS
                     VPGPAVDRPQFAATQPDVEGLVDGLTGDGLSGYLKQSVEWSPCDEDECARILAPLDYA
                     NPAAAAVTLSLRRQPATKSPRLGTLFVNPGGPGASGTDLAESFDNDGLEQYDILGWDP
                     RGTGASTPVQCFSDRQADAFNNLDGSPDDEAERAALIEGVFDFGKSCWERSGSLLEHI
                     STIETVRDLDLMRELVGDAKLSYLGYSYGTQIGATYAELFGEFTGRLVLDAAVNITDT
                     DEVIQAMGFDLALGNFASWCAKEACALGSSKDEVLDLITGLLDKLDSAPLKVGERKLT
                     QTLAATGIAALLYGGKSTWPTLAASVLAATKGRGDALLWGADFLNSRDEDGTHDPMFF
                     AFPAISCADDSTEQGVLDADRLWAEDQQKAPIFGKYFGPQYTCALWPVRPARRLDIRG
                     ASAQPLLVIGGTGDNATPYQQAVDMADQLKSATLVTYEGEGHGTFGGKSECVDRIVVN
                     YLVKGTMPAEGVRCS"
     gene            complement(4592..5428)
                     /gene="ricT"
                     /locus_tag="PKJ61_04725"
     CDS             complement(4592..5428)
                     /gene="ricT"
                     /locus_tag="PKJ61_04725"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HOC13161.1"
                     /translation="MPRVLAVAYEPLGRLYYFDPGEEHVSFGQQVLVPSGAGAEVATC
                     VWGPVTLEWDTELPRLIGIAQPSDLDRDARNRAKRAEIAAVARRLIAQHDLGMRVVGV
                     DYVDRSEEFDQLAVVYFEAPGRVDFRALIPDLARALKARIDLRQVGSRDAAAILGGVG
                     ACGREYCCASIGLPEAVISMRLARTQDLPANPAQLLGGCGRLMCCLAYEHDLYREFRE
                     RAPGLGARVTTAEGPGRVVGHVVPLEAVMVQIGHEQVRCPLASIGSPMDPPRPARKAP
                     PG"
     gene            complement(5519..6796)
                     /locus_tag="PKJ61_04730"
     CDS             complement(5519..6796)
                     /locus_tag="PKJ61_04730"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HOC13162.1"
                     /translation="MTEPRDFEPVVDADVPAPDATPVSDTPEPELSTWRPVQPAEPAA
                     VDETPAQPEASAQFETPAQAEPFAGPEPTIEAPAVAEPVLESQPPAAQPEVVPAPLDE
                     PESVSVPEPEPVATPEPEPVATPEPEPAPVPEPVAVPEPAPTQARTPEPQPVVAPEPE
                     LVIDSEPTTQAETVAIVPAEPETAETVAIRPVAGPPMVEPTIALPNEPAPTAAVPTES
                     IFRPVSPSQEPTTVEQLSDEEAKLAAERAARRDARVAALAATAPMAIAAPAPVIVHKR
                     TNDKFWGSLGLFLLRLVVAVIFAIRGLNIVTDIPAAQATFAKTILPEPQLMAIVTGVA
                     SLLIALSLVLGLLTRLAGLGIALIAGGVLAFVLWGPWSPFVAGQPGFLGEFELLLAAV
                     GLLFLLIGGGGWSLDRSFRAGRERDKAERNAAS"
     gene            complement(7000..8178)
                     /locus_tag="PKJ61_04735"
     CDS             complement(7000..8178)
                     /locus_tag="PKJ61_04735"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012918265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HOC13163.1"
                     /translation="MNSTSTFADGVWTELIGQDRVVEVLQRAVRGAGADGNRHAMTHA
                     WLFTGPPGSGRSNAARAFAAALQCQFGGCGDCNTCLTALSGAHPDVTLVRTEQLSIGV
                     DEVRDLVRRSAMAPTLGRYQVLVVEDADRITERGADALLKSIEEPAPRTVWLLCAPTS
                     NDVVATIRSRCRLMELSTPSTAAIAELLQRRDGVEPQLAGFAARVAQGHIGRARALAR
                     SETARARRADVLALPGRLTSLGACLSAAASMVDAATEEAAAVTSELDAREKAELSEAL
                     GFGTRGARPRNAQAALRELEDQQKARAKRLQRDALDRVLTELTAFYRDVLAIQTGSGA
                     ALINAEIADAIGIAARRGSADATVARLDAILACRTALETNVAPLLAMESLLLALADWH
                     "
     gene            complement(8175..8813)
                     /gene="tmk"
                     /locus_tag="PKJ61_04740"
     CDS             complement(8175..8813)
                     /gene="tmk"
                     /locus_tag="PKJ61_04740"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOC13164.1"
                     /translation="MGEGVFIVFEGGDGVGKSTQATLLAEWAAGQGREVVVTLEPGGT
                     AIGRVLRDLVLNPSWGDVSPRAEALMYAADKAQHVYEVVKPALGRGAVVISDRYVDSM
                     LAYQGAGRVLSVERVEQVARWATEDLLPDLTVLLDLDPDHGLATIAEKDRLEGAGTDL
                     HVRARQFFLDLAARDPEHYLVLPARTGIESIAAAVRERVGELLSVPSGKLTP"
     gene            complement(8933..11653)
                     /gene="topA"
                     /locus_tag="PKJ61_04745"
     CDS             complement(8933..11653)
                     /gene="topA"
                     /locus_tag="PKJ61_04745"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013161892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HOC13165.1"
                     /translation="MATEHKSRLVIVESPTKAVSLAKFLGSGFVVESSRGHVRDLPTG
                     AAEVPAKYKGEKWARTGVNIAADFEPLYVVAADKKGTIRDLKAKLKNADELLLATDED
                     REGEAIAWHLLDELKPKVPVKRMVFHEITPEAIHAAVENTRELDTDLVDAQETRRILD
                     RLYGYEVSPVLWKKVMPKLSAGRVQSVATRLVVDRERERIAFRAAAYADIEAVLDAGV
                     DADPRTFTAKLTAFGSRRIAQGRDFDSVGKLAGKDLLQLGIDAAQRLASDLAKAGFSV
                     GSVEAKGYTRKPYAPFRTTTLQQEAGRKLGFTAQRTMSVAQELYERGFITYMRTDSVT
                     LSSTAIEAARAQVKELYGGKYLPAQPRVYASKVKSAQEAHEAIRPAGATFRTPQQTGL
                     HGDQFRLYELIWKRTIASQMKDAEGETVSVRIGATLPERVSVTDAATGEVVDSGRATF
                     NASGRTITFLGFLKAYVESVDDDSSTDDAQARLPQLASGQRLEAETISAQAHETKPPA
                     RYTEPSLVAKLEELNIGRPSTYAAIIRTITSRDYVFKKGSALVPTWLAFAVTRLLEEH
                     FAKLVDYAFTAEMEQTLDEIAAGDAARVAVLKDFYFGPEGSAHEGLEKLVTELGEIDA
                     RKLSTFNLGDDVDVRVGRYGTYVEDSEGHRANVDPDLAPDALTLDFARELLAKPAIEE
                     RTIGIDPASGNAIVAKDGRFGPYVTEVLPEDAPKSAKPRTGSLFASMSTETVTLEQAL
                     QLLSLPRLVGKAADGEEITAQNGRYGPYLKKGTDSRSLTSEDQIFEITLADAEAIYAQ
                     PKTRGRAAAASGPLRELGADPVSGKPVVVKDGRFGPYVTDGEFNATLRRTDSVEAMTL
                     ERAAELLAEKRAKGPAPKRTTARRTTTTKKATTTKKPATKKTTTTTKPAK"
     gene            complement(12196..13968)
                     /locus_tag="PKJ61_04750"
     CDS             complement(12196..13968)
                     /locus_tag="PKJ61_04750"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOC13166.1"
                     /translation="MAELDDIRPDAPQGADATEPTQVLPSVPVPPLPAASAPTSPWAR
                     PADLSGNWAAPDATKRFDATTPNAPAAANPTTRIPSAATGPTTPIPTTPGGGQYPTAQ
                     QYPGQAYGQQPYFRPGAGQQQAGYYQPGYYGQQYSQQPYGQQVYGQQPHGQQPHGQQA
                     YGQQAYGQQAYGQQPGHAQYGPGYQQDAQRPAQSAYGQSPYGPNGYYQPQHTTTPTKK
                     RWPIAVLAMLAAFLLIASGSFWAARQFDGALPSSSRTTQPTIEPYYPRDEPSEDPAQI
                     QPTQPEEETGGSATSNGVSDGQAAGVVLIEAETASGLAAGTGMILTADGRVLTNYHVV
                     AGSTKLAVTDAVSGNTYSATVVGFDQTKDVALLQLKDASGLNTVTIDDDPVDVGDAVA
                     AVGNANGGGELVKAPGEVTGTDQDLTVSSDSPWGNTEDLTGLIETDAGAVPGDSGGPM
                     FDDENEVLGMTTAGSTREGTSYAVPIATALAVVEQVESGKDAGTVRVGPAGFLGIKVA
                     DADQRSAGKTITEVVADSPAAKAGVKAGSTLTRVGDTTIKASTNLATVIRALEPGQQV
                     SIEWTASNGSQKEATVTLGSSPVN"
     gene            complement(14067..15518)
                     /locus_tag="PKJ61_04755"
     CDS             complement(14067..15518)
                     /locus_tag="PKJ61_04755"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOC13167.1"
                     /translation="MLTDSAIAQLRADVLAADYTLDGLTERLGSAAIAALARNTSVAA
                     ARALGDATDPQADAIRLWLLQLPVPAQRLANWTSLPALKAAGLVVGDAELRATIELKP
                     HGSDRREGWICSDHTPLDERVPQPRPDFVLGASPASTTLTQLVPPRRYPRVLDLGTGC
                     GIQALHLDAAEIVATDLNPRALELARIGLGLSNVEAELRLGSLYEPVAGERFDLIVTN
                     PPYVLSPPSEAKLVYREGGFSGDGLMRTVVAQAADHLTDDGLLIVLGNWAVTDPEQWE
                     QHLKEWIPDGCDALVLQRELLDPYEYIEIWLTDAGLQGTDEYASRYAEWLDYFVSLRI
                     VGVGMGWIGLAKSKCPAPVVRIEEWPHAVHQPVGQAFSDFFTAIPTTQLTDAELLATS
                     LRLHPGVVQETIGMPGAAHPQHIVLRQQFGFGRAVEAGTALAAIVGACDGDLPLGVLI
                     GAVAGLLDTDTVELAAEVLPQLRRLVADGFVVA"
     gene            15665..16315
                     /locus_tag="PKJ61_04760"
     CDS             15665..16315
                     /locus_tag="PKJ61_04760"
                     /inference="COORDINATES: protein motif:HMM:NF015400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HOC13168.1"
                     /translation="MRIVLLGASGNTGREITRLLSPMLAEDDQVVLAGRDRGRLEATA
                     EVVSGPAEVELRQVDATVDSAVRDLVEGAQQVVVTASLPDRVGALAQIVADAGADWID
                     TLLSSPAKLAALRALEPVLRARGRCFVTDGGFHPGLPAAMVRWAGDQVDELITADVFG
                     GMRIDWRADSLAESTVAEMLTEFGDFDLSTWVDGGTRKLRMSECPKVDFGPPTDRE"
     gene            16458..18701
                     /locus_tag="PKJ61_04765"
     CDS             16458..18701
                     /locus_tag="PKJ61_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013161894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOC13169.1"
                     /translation="MAVPAWLLDDPRLRHHHHRAAAHGSTADFPDWLSERLREPLSAQ
                     GITRLWEHQAVAAEAAFAGRHVAMATGTASGKTLGYLLPVLAATADGTVGFDIDHGPL
                     DLGRRHSAIYLAPTKALAHDQARVCRSLALPGWRTTTLDGDSSTEERRFARDFGRYVL
                     TNPDMLHRSVLPRHQQWTRLLSTVRYVVIDEAHRYRGVFGAHVAAVLRRLRRLANHYG
                     ADPVFVSASATVADAGGLLSLLAGVDDVLEVAADTSRRAALDYLIWQPEADPHHEAAD
                     MLARLVSEGRQTLAFTTSRVQAELVALRAQRKGGDDSDIQPYRGGYLAEDRRELEAGL
                     QSGRLRGVACTNALELGVDISGVDAVLTCGFPGTRAALWQQAGRAGRAGRDALAVLIA
                     RPDPLDAFFCEHPEAIFDAPVERTVLHPENPNVLAPHIAAAAAELPVTLADRKWFGET
                     LPALLDGLTAAGHLRKRPGGWFWTRSDRPADAIDLRSIGGASVEVVETVTGRVIGQVD
                     PAAADRTVHTGAVYLHQGDQWRVLEYDPAGHTALAQRVQEGYFTQALGASDVRILGVQ
                     QERVLGAGRVYFGAVELSSQVTGFLRRDEASGKVWDSHPLDLPQRTLRTQAVWYTVPP
                     AALGGIPVRDLPGAAHAAEHTAIGLLPAFAPCDRWDIGGLSTALHADTGQLTVFVHDG
                     HPGGAGFAQRGFESVEAWLTATRDRLLQCPCAAGCPACVVSPKCGNGNQPLEKAAAAS
                     LLALLIT"
     gene            complement(19162..19554)
                     /locus_tag="PKJ61_04770"
     CDS             complement(19162..19554)
                     /locus_tag="PKJ61_04770"
                     /inference="COORDINATES: protein motif:HMM:NF041390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE family type IV pilus minor pilin"
                     /protein_id="HOC13170.1"
                     /translation="MNYRKRVCGSWNWKASLGQRDRQRGMVTAEIAFASIGAALATVG
                     LAWVLVVLALLVRCQDTAAEVARQEARADRSAVAKAIADRPTGAQVLVGNHGNEVRVE
                     VRLDARPWAAWLPAIPVSATATVIREPA"
     gene            complement(19758..19952)
                     /locus_tag="PKJ61_04775"
     CDS             complement(19758..19952)
                     /locus_tag="PKJ61_04775"
                     /inference="COORDINATES: protein motif:HMM:NF025395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4244 domain-containing protein"
                     /protein_id="HOC13171.1"
                     /translation="MAGQLVRRHNERGMTTAEYAVGTVATVSVVGVIIGILNNPDFQQ
                     IIWQILKAIIAVILQAISGS"
     gene            complement(20178..20900)
                     /locus_tag="PKJ61_04780"
     CDS             complement(20178..20900)
                     /locus_tag="PKJ61_04780"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOC13172.1"
                     /translation="MSGTWLAALSVALAVLAWGSPSARLAAGLVAPTGRRRLIQPSQR
                     LGMAFGTAVSALLLTAGLGWWALPAAAAIGVASYLVLGRLETSNTVRRNAELVAGLPQ
                     VCDLLAVCLEAGLPLRAAVAVVGDAVDEPLRGVLGQIGGQVGLGTDEGAAWADAAAAE
                     PALAGLCREVARSVVSGTTLAKTMRALGADARRESLAAKEVRAKRVGVRSVLPLMVCF
                     LPAFLLLGVVPIIGGVAAHLFG"
     gene            complement(20897..21706)
                     /locus_tag="PKJ61_04785"
     CDS             complement(20897..21706)
                     /locus_tag="PKJ61_04785"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOC13173.1"
                     /translation="MTAIAVLAAFLAGWVGFAPSAVTRLRPVEGGAAWSPSSQRRLGA
                     AFPLVVVAVASIIGLMVWGGAGGSVAFAVTLPLVTAWTVWRRHRRRSTAAATARDVAT
                     ACQQLAGLLRVGHVPGVALRLAAQDAPVLADAAAIHAIGGEVAAALRRQSAVGGYGGL
                     AQLGIAWDVAERTGASLTATLDALAERLAADRAVHNVVAAELSGPRATGRLLAVLPLV
                     GLGLGYGFGGDPLEFLTASVPGQLCLALGALLGCVGVFWTERIAGSEEDLS"
     gene            complement(21703..22845)
                     /locus_tag="PKJ61_04790"
     CDS             complement(21703..22845)
                     /locus_tag="PKJ61_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013161907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadA family conjugal transfer-associated ATPase"
                     /protein_id="HOC13174.1"
                     /translation="MPEPDLDAVRTHLGRLGQQPGPVEVGAALRSLGMLVSDSAVRST
                     VAALRRESVGAGVLEPLLALPGVTDVLVNGPDHVYLDRGGGLEPAGVRFASEAELRRL
                     AVRLAASVGRRLDDGSPFVDARLPNGVRVHAVLPCLADTGTCLSLRVPNRQRLGLHDW
                     VASGSVSADAAKLLAGLVAARRAFLISGGTGSGKTTLLGAMLAQVPPDERIVVVEDSR
                     ELQPEHPHCVRLEARPPNAEGAGAVTLTDLVRQALRMRPDRIVVGEVRGGELADLLRA
                     MNTGHEGGCGTVHANSAADVPARLEALGALGGLERDALHAQLGAALRVLIHVRRLPAG
                     RRWVDEIQLVEPSPDGRCRTVPAVRFSAEGAVPGPGFDELMALAAS"
     gene            complement(22838..23887)
                     /locus_tag="PKJ61_04795"
     CDS             complement(22838..23887)
                     /locus_tag="PKJ61_04795"
                     /inference="COORDINATES: protein motif:HMM:TIGR03815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13175.1"
                     /translation="MAEASSGRDVLVVAGDGETQDVILSAVAALALDAEVVSDLERIG
                     QPWRAAGTVLVASDQAARLAARALPRRDGVFLVGSDASELSRWSAPLSARVIQLPDGG
                     AWLGAVLSDRTSRDRAPVVTVLGASGGVGASTLAAALAQLAADSGGGAALVDVDPLGG
                     GIDLLLGAERTDGWRWPRLNGADGQVGDLRPYLPVVEQVSVVSMARTATLDLAREPLA
                     AIVGSLRTWHSLVVLDAGRSLGPACREAVRLGRVLLVVSAGVRGVAAARAAVEVLGLD
                     GAELVLRRRRGGLAADVVADAIGLPVLAELPEEPRLVAAAELGEPPARGGRRRYRASV
                     ARVLTAVRSEEPGHA"
     gene            24035..24811
                     /locus_tag="PKJ61_04800"
     CDS             24035..24811
                     /locus_tag="PKJ61_04800"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOC13176.1"
                     /translation="MATPGAFPAAAVAWLAPTRPGSLLAIGSASAPTATHFEQLGNRV
                     TITARAPEDVRRACARHTGFHGVAAAPDALPFLPCSFDAVLVAQGMHRLPPGLVLAEF
                     ARVLVPGGQLAVVATFRDDSVPWVRRLAAILQRYDPDAMRGADESAATIPASAHFPEV
                     EQRDFRRWVPITRDGMLKLVSSAPKLANLDIGVAEPLLAEVAELYDSSAPRAEPLLLP
                     YAVRCSRAEVDHAELSSVLRLPEDGLQINLQSRPFVSDSD"
     gene            24845..25315
                     /locus_tag="PKJ61_04805"
     CDS             24845..25315
                     /locus_tag="PKJ61_04805"
                     /inference="COORDINATES: protein motif:HMM:NF018980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HOC13177.1"
                     /translation="MADQSGIADVIKSIQDDVRTIVKGELELAKAELLPQAKSAGIGA
                     GLFGGAAYLGIMGATLLFCGLSFWLSFGFQSWFGIDLLAALAWGFAVMAILMFLLAGV
                     LSLIGKQRLNLPGPQATTASAQASVDAVKGAITKGQERVAATSILGGEPRKELE"
     assembly_gap    25452..25551
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(25603..26259)
                     /locus_tag="PKJ61_04810"
     CDS             complement(25603..26259)
                     /locus_tag="PKJ61_04810"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002517131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HOC13178.1"
                     /translation="MSFPVPVPDGLRAAAAQFDRPDALSFIQGARTPQGKRRAAVLIL
                     LSGEPNAYEIVLVEKRPDLRSHAGQIAFPGGSLEPQDLNPVAAALREANEEVGIDPSA
                     VVVLGSLPDAHIPRSGFDVTSVVGWWGEPVRLQVVDVGELAGVHQVPVSRLLDPANRS
                     TWAHPGGFRGPAFTLDDLYVWGFTAYLLDGLFDLLGWTQPWATDLVTEIPPRFLSERL
                     "
     gene            complement(26256..26843)
                     /locus_tag="PKJ61_04815"
     CDS             complement(26256..26843)
                     /locus_tag="PKJ61_04815"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOC13179.1"
                     /translation="MALLLTLAGCSPSVPSANRFPTPFESYSPATPKTVDLVTARKAA
                     GIADCPKPSEAPVVPGGLPDLTLDCLGGDSQVRLAGLRGPMLINLWAQWCEPCRAEAA
                     DLSAFASKQEDVAVLGINYSDPQPELAIEFAQLMAMKYPQLVDEEATIRGPLGVAGIP
                     HTVLIDADGVVVARHPGIFTSLEEIESWVAAGLAA"
     gene            complement(26951..27652)
                     /gene="nth"
                     /locus_tag="PKJ61_04820"
     CDS             complement(26951..27652)
                     /gene="nth"
                     /locus_tag="PKJ61_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOC13180.1"
                     /translation="MTATESRLALVRRARAINRGLGELYPDAHCELDHASPLQLLVAT
                     VLSAQSTDRRVNTVTPALFARYPDAAALASADRTELEELIKPTGFFRAKTDALIRLGT
                     ALVADHGGVVPGRLAELVKLPGVGRKTANVVLTDGFGVPGITTDTHFIRLSQRFGWTA
                     SAKPEVIEAEVGELFPKSDWIRLNHRVIWHGRRRCHARRPACGACSVASYCPSFGEGP
                     TDPKVAAKLVREPRG"
     gene            complement(27649..28107)
                     /locus_tag="PKJ61_04825"
     CDS             complement(27649..28107)
                     /locus_tag="PKJ61_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012918130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOC13181.1"
                     /translation="MSHSARLAELGIALPTVPAPVAAYVPATISGGQVLTAGQLPFVD
                     GKLTDTGKVGAQVSPERAAELARTAALNAVAAAASVVGGVDGIARVVRLVVFVASDPG
                     FTGQPAVANGASLLLQEVFGEAGIHVRSAVGVAVLPLDSPVEIELVVEAA"
     gene            complement(28104..28262)
                     /locus_tag="PKJ61_04830"
     CDS             complement(28104..28262)
                     /locus_tag="PKJ61_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HOC13182.1"
                     /translation="MTKWEYVTVPVLVHATKQILDNWGADGWELVQVVPGPNPQNLVA
                     YLKRPVVE"
     gene            complement(28333..28611)
                     /locus_tag="PKJ61_04835"
     CDS             complement(28333..28611)
                     /locus_tag="PKJ61_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016669081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HOC13183.1"
                     /translation="MPLGVEDWTLQAKCRGLEDQLFPDGADQKRVRSVCYTCPVRNEC
                     LAEALDNRIEWGVWGGMTERERRQLLRQRTDITSWTSILCPKHSRCDL"
     gene            28790..31150
                     /locus_tag="PKJ61_04840"
     CDS             28790..31150
                     /locus_tag="PKJ61_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015071580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HOC13184.1"
                     /translation="MGKRLYSLVMFLVVAIIGGLLAAGLVVPIAGMATDTTTSMVESL
                     DQLPAELETPPQSERSRLLNADGSLLAYFYNENRVYKELKDISPIMQKAQVAIEDHRF
                     YEHGAMDLQGTLRALVSTSQGNTQGGSSLTQQYVRLVLVENAEAKNDKAGVIAAKENT
                     VARKIRELRYAIAIEEKLSKDEILQNYLNIAYYGDGAYGVEAAAKHYFGVSAAKLDLA
                     QSAMLAGLVRNPNATNPVSHVDLAIERRNNVLDRMLELKLITSDEAAAAKAVEFDQSK
                     VKDYLAGCANSKFPFVCDYAYRTLLKTPSLGSTEEERKQLIYRGGLTIMTQIDPAAQK
                     KAEKTIANYVSAKDPVVSVITMMEPKTGLIRAMAQNRSEWGNNWKKGQTNWNYAVGAD
                     MGGADGYQGGSTFKAFVAAAALEENMGVNFTVRVKRSVNYQNKIFPTCNGDLKVRDDW
                     TVDNPESGTMNLLQGTKYSVNNYYVALEQAVGLCAVTNMAKRLGLKASDGRDFVETEQ
                     VPSFTLGIVEVTPLSMVEAYGTFANRGVHCNPIILKSINGKDGAAFDVPTAGCKRVLS
                     EEVADAVNKVFQGPYTPGGTAYTAKVPGYTMAGKTGTVPKNKAVWTVGYTPDLVGAAV
                     ISYDNNKNYRKFWKGKPSFLRYVKLPASGRWLSGFSGNNAGANLLKPAMTAALADIEE
                     RTSFVEPPAEVLRGQTVGIPSCAGMGAAGCAATLRRAGFSSFTYTVKSDKPKGTFLGT
                     NPSGGAPKGSQIGLMVSQGPKKKEEPTPEPSASASPQEKVKEQPRR"
     gene            31147..32058
                     /locus_tag="PKJ61_04845"
     CDS             31147..32058
                     /locus_tag="PKJ61_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015011782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOC13185.1"
                     /translation="MIGKVIGGLAAGAAVCVGYGVLCEANAFRVRRVRVPVLPTGAPK
                     IRVLHLSDIHLARGQRKKLAFVSRLAALEPDLVISTGDNIAHGDALEPLLAVLRPLLE
                     VPGAFVFGSNDYVEARMSNPLAYLARSTSHDGVREELTPLPTEALRAGLASGGWADVT
                     ALRATLHVRGVQLEFRGTDDAHLHRDDYSLVAGPAASGTDLSIGVTHAPYLRVLDGMA
                     ADEVPLVFAGHTHGGQVCVPGWGALTTNCDLPPAMAKGLHEYVGRDGGRSWLHVSAGI
                     GSSPYAPYRFACPPEVTLLTLTPVPNG"
     gene            32112..32188
                     /locus_tag="PKJ61_04850"
     tRNA            32112..32188
                     /locus_tag="PKJ61_04850"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32146..32148,aa:Pro,seq:cgg)
BASE COUNT         5271 a        11214 c        10693 g         4936 t
ORIGIN      
        1 gctgcgcggc cggcagaacc tgctcggtca gttcctgcgc ttcatcgcct ttgacctgcg
       61 gatcatgcgg atggttgcga agggccacca ctgattaaat gcatttagcg gacggcgctt
      121 atcgggccgt ccgctaaatg caagtctggc gaccccgaaa agtcaggggc gtacgctagt
      181 cgacaccgca atcgaaagca aatcgccggc gccgcttctc aattaaaacc ggacgccggt
      241 tcgcttgacg attaggccag ggagaggaac agcttttcca gggacgaagt gtcgactccg
      301 gtgccttccg gatcggcggt gatgcactgc cgcagcccga ccgcgatgac cgcgaaccca
      361 gcccgatcga gcgccttcga cacggcggcg agctgggtga cgacctcctt gcaatcgcgg
      421 ccttcctcaa tcattcgaat gataccgccg atttggccct gagctcgctt aagccgcttg
      481 agcactccaa ccagatcgct ttcttcgagc tgcattacga aattgccttc cacccgttca
      541 gtggattcca ctgtaccggc atggatcggt tcttcaggca ttgctggctt ccgagttttg
      601 ctcatcgagt tcggcgcgta ccgcgtccat gtcggccgcg cggacggcac tgatcagctc
      661 ctccagcgcc ggcgcgggca tggccccagg ctgggcgtag accagaatgc cttcgcggaa
      721 gaccatcagc gtcgggatcg acatgatcct cgctgcggcc gccaggtcac gttcggtctc
      781 ggtgtcgacc ttgccgaaga ccaggtcagg gtgctgctcg gaagcggcct cgaatacggg
      841 ggcgaacatg cgacacgggc cacaccaggc tgcccagaag tcgaccagta cgatgtcgtt
      901 ctcagcgatc gtctgctcga aaaccgccgc cgtcagttca gtcgttgcca tgccagaatc
      961 ccctcaagag ttataccccc aagggtatca tttgccgccc gccggccccg ttgagccgat
     1021 gtcctcgtcc gggccgtccg gcggcggcaa caggtgggtt ccgatcggcg ggttggtcgc
     1081 gaagccgaag gcccgcagct cgatctcctg gtcctgagcg gtgagtcgct gctcgtgctg
     1141 gtgcaggtgt tcctgccagg acggcaccgc gaagatctcc aggtaggtgt cagcgcgctc
     1201 ccctacccgg tagagctccc agcgcatcgc accactgcgc atccgcgagc gccgcaggtc
     1261 ctccatcgcg gcccgccagt cggccacccg gtcggaaggc acgacgtact cgaccatcac
     1321 ctgcaccggc ccagcgtccg gctcgggcga gtagagcacc tgggcatcac cccagtagac
     1381 cagcggcgac cggtccagct gggagctctc cgggaagtgc caggccaagc cggcgaggcc
     1441 ccccgcagcc agtagtgcgg ccgccgccca gaccgcggcg gacaggctca catgctgcgc
     1501 gaccagtccc cagaccgggg ccgcgacggc ctgcacaccg gcgaagacca tcaggtagac
     1561 cgcgagggcc cgggcgcgca cccacccggg caggtagagc tggagctcgg cgttcaaggt
     1621 ggagatggtt gccgtccagg tgaagccgag gatcgccagt aacgggaccg cagcgaacag
     1681 tcccggaacg accatcgtga gcgcgaaggc gccagcgaac aggacggcgc tccaactgag
     1741 cacctggtta ccggagaagt gcttggtaac ccgtccgagc gacagcgccg cagcaaccgc
     1801 gcccaagccg aggaaggcga acagcaggcc gtaaccgctc gcaccgaggc cgagctggcg
     1861 attcgcgatc agcggcagca gcgtccacat ggccgtgccg gggatcacga acacggcgag
     1921 ccgacccagg atcagccgca ctaccggctc gtgccgcacg tagcgtccgc cggcgcgaag
     1981 ggccggcagg aagcgctcgc gggcgcgcgg ggactcgtcc ggacgtcgcc aggccagcaa
     2041 tgcgccgatc aggaacaggg cgctgaccgc ggtgagcagg aagaccagcg ggacgcccca
     2101 agccgcgatg atgagcccgg ctatcgccgg gccaaccgcc cgcgcgacgt tgacgctgac
     2161 catgtcgagg cgggtggcct gcgcgatctg ggagcgcggc accagctcgg tgatcagcgg
     2221 ctgccaggtg ggcacctgca tggcggtgcc ggcgccgatc aggaaggtga aggcgagcag
     2281 gagcagcggg ggcatccggt cgagccaggt gagcacggcg aggatgatcg cgacagtgcc
     2341 cgagtagagc tggacgaaga gcagcagccg gcgccgatcg aacgcgtcgg ccagcgcccc
     2401 tgcggcgagt ccgaagagca gcatcggcag cacggacgca gtctggacga gtgcgacgat
     2461 cgtagcggcg ttcgggtcgc tgatgaacaa ccactgcgcc ccgaccatct gcgcccacgc
     2521 gccgacgctg ctgatcacca caccaatcca gagccacttg aacgcccggt tgccgaatgg
     2581 ggcgagcgcg gagacgtgct cagtggtgcc agtgccggtc atcggcgcag cgttcggttc
     2641 agccaacgca tacctcacca catctggagg cccaccggga gagctgggcc attggttgca
     2701 gggcttactc cccacgacga cggagccacc tgcgggaatc gaacccgcga cctacgcttt
     2761 acgagagcgt cgctctaccg actgagctaa ggtggcgagg cttttcagcc gaggcacaac
     2821 tataggggca agccgcccaa gctgccgaaa cgacgcccca cgcgtcccac gacctgctgg
     2881 atggtcgagt tcgtcaggac tgagtgcggc ggaaagcggt ctcgacaagc tcgaccagcc
     2941 gaaggactcc ccagccgagg gctcggacgc cgacaagtca gctgcagcgg acgccttccg
     3001 cgggcatcgt gcctttcacc aggtagttca ccacgatgcg gtcgacgcac tcggacttgc
     3061 cgccgaaggt gccatggccc tcaccctcgt aggtcaccag cgtggccgac ttgagctggt
     3121 cggccatgtc cacggcctgc tggtaggggg tcgcattgtc ccccgtcccg ccgatcacca
     3181 gcaggggctg cgcgctggcg ccgcggatat ccagccgacg ggccggccgc accggccaca
     3241 gcgcgcaggt gtactgcggg ccgaaatact tgccgaagat cggggccttc tgctggtcct
     3301 ccgcccatag ccggtcggcg tccagcaccc cctgctcggt ggagtcgtcc gcacagctga
     3361 tcgcagggaa tgcgaagaac atcgggtcgt gggtaccgtc ctcgtcgcgc gagttcagga
     3421 agtccgcgcc ccacagcagc gcgtcgccgc ggcccttcgt cgccgccagc acggacgcgg
     3481 ccaacgtcgg ccaagtgctc ttgccgccgt acaacagcgc cgcgatcccg gtggcagcca
     3541 gcgtctgggt gagtttgcgc tcgcccacct tcagcggcgc cgaatccagc ttgtccagca
     3601 ggccggtgat cagatcgagc acctcgtcct tgctggaacc gagcgcacac gcctccttgg
     3661 cgcaccagga cgcgaaattc cccagcgcca agtcgaagcc catcgcctgg atcacctcgt
     3721 cggtgtcggt gatgttcacc gccgcgtcca gcaccaggcg tccggtgaac tcgccgaaga
     3781 gttcggcgta ggtcgcaccg atctgcgtgc cgtaggagta acccaggtaa ctcaacttcg
     3841 cgtcgcccac cagctcgcgc ataaggtcga ggtcgcggac ggtctcgatc gtggagatgt
     3901 gctccagcaa cgacccactg cgctcccagc acgacttgcc gaaatcgaag accccttcga
     3961 tcagcgcagc gcgttcggcc tcgtcgtcgg gtgaaccgtc caggttgttg aaggcgtccg
     4021 cctgccggtc gctgaagcac tgcaccgggg tggacgcgcc agtgcctcgc ggatcccagc
     4081 cgaggatgtc gtactgctcc agtccgtcat tgtcgaagct ctcggccaga tcggtaccgg
     4141 acgcgcccgg cccgcccggg ttgacgaaaa gggtgccgag ccgcgggctc ttcgtggccg
     4201 gctggcgacg cagtgacagg gtgaccgcgg ccgcggccgg gttcgcgtag tcgagcggag
     4261 cgagaatccg tgcgcactcg tcctcgtcgc acgggctcca ctccacggac tgcttgagat
     4321 agcccgagag cccgtcaccg gtcaggccat ccacgaggcc ttcgacgtcc ggctgcgtcg
     4381 cggcgaactg cggacgatcc accgccggac cggggacgga cgagattcga atcccattct
     4441 ccggaaccac ggacgcggac ggcgcggacg gcggcgccgg cgtcggcgtg cacgcgccta
     4501 gcgcgagcgc cagcgcgatg acgacgaggc ggcggatcgt agaaggcact cgttcagggt
     4561 agccaaaccc cccgaacgcc ggtttcatgg ttcacccggg cggcgctttc ctcgcgggtc
     4621 gtggcggatc cattggactg ccgatactgg ccagcggaca gcgcacctgc tcgtgtccga
     4681 tctgaaccat caccgcttcc agcggcacca catgtccgac cacccgtccg ggcccctccg
     4741 cggtggtcac cctggcaccc agtcccggag cgcgctcgcg gaactcgcgg tacaggtcat
     4801 gctcgtaggc gagacaacac atcaagcggc cacaacctcc caggagctgc gccgggttcg
     4861 cgggcaggtc ctgcgtccgc gcgagccgca tcgagatgac ggcttctggc agcccaatcg
     4921 aggcgcagca gtactcccgg ccgcacgcgc ccacgccgcc caggatcgcc gccgcgtccc
     4981 gggatccgac ctggcggaga tcgatccgag ctttgagcgc acgcgccaaa tctgggatca
     5041 gggcccggaa gtccacccgt ccgggagcct cgaagtagac caccgccagt tggtcgaact
     5101 cctcgctccg atcgacgtag tcgacgccca ccacccgcat gcccagatcg tgctgggcga
     5161 tcagccgccg ggcgaccgca gcgatctcgg cccgcttcgc ccgattgcgg gcgtcccgat
     5221 cgaggtcgga cggctgggcg atcccgatca gccggggtag ctcggtgtcc cactccagcg
     5281 tgaccgggcc ccagacgcag gtggccacct cagcgccggc cccggacggc accagcacct
     5341 gctggccgaa gctgacgtgc tcctcgccgg ggtcgaagta gtagagccga cccagtggct
     5401 cgtaggcgac agccaatacc cggggcatag cgcaaggcta aggcttgaga acctggtccg
     5461 agtgtgatca ggacaagctg acgacagtct cagcaagcgt tcccgactat gcggttcgct
     5521 aactggcagc gttgcgttcg gccttgtccc gctcgcgacc ggcacggaag ctgcgatcca
     5581 ggctccagcc gcccccaccg atcagcagga agagcaggcc gaccgcggcg agtagcaact
     5641 cgaactcacc gaggaagccc ggctgaccgg cgacgaacgg gctccacggc ccccacaaca
     5701 cgaaggccag caccccgccg gcgatcagcg cgatgcccag gccggccaga cgggtcagca
     5761 gtccgagcac gagtgacagg gcgatcagca gggacgcgac cccggtgacg atcgccatga
     5821 gctgcggctc cggcaggatc gtcttggcga aggtggcctg cgcggccggg atgtcggtga
     5881 cgatgttcag cccgcgaatc gcgaagatca ccgccaccac cagtcgcagc aggaacaggc
     5941 cgagcgaacc ccagaacttg tcgttggtgc gcttgtggac gatcaccggc gccggtgcgg
     6001 cgatcgccat cggcgcagtc gcggcaagcg cggccacgcg ggcgtcacgg cgggcggcgc
     6061 gttcggcggc cagcttggcc tcctcgtccg acagttgctc gaccgtggtc ggctcctgcg
     6121 aggggctcac cggacggaag atcgactcgg tgggcacggc tgccgtcggt gccggttcgt
     6181 tgggcagcgc gatcgtcggt tcgaccatcg gcggcccggc aaccggacga atggccacag
     6241 tctctgcggt ctccggctcg gccggcacga tagccacggt ctccgcctgg gtggtcggct
     6301 cgctgtcgat caccaactcg ggctcgggcg ccaccacggg ctgcggctcg ggagttcgag
     6361 cttgcgtcgg cgctggttcg gggactgcta ccggctcggg aacaggtgcc ggctcaggct
     6421 cgggcgtggc gaccggctca ggctcgggcg tggcgaccgg ctcgggctcg ggcaccgaga
     6481 ccgactcggg ctcatccagt ggcgcaggga ccacctcagg ttgggccgcc ggcggctggg
     6541 actccagcac cggctccgcg actgccgggg cctcgatcgt cggttccggc ccggcgaacg
     6601 gttccgcctg agcgggagtc tcgaactgag cggatgcctc gggctgggcg ggcgtctcgt
     6661 ccacggctgc gggctcggcc ggctgcaccg ggcgccaggt gctcagctca ggttcagggg
     6721 tgtcgctgac cggagttgcg tccggtgcgg gcacgtcggc atcgactacg ggctcgaaat
     6781 cgcgcggctc ggtcacagga agtcctctca acaagggcac actctcgtgt ccatgcttgc
     6841 atccgtcgac gcggacggcc gtgagcgaca cggcgcaggg cgcgctctgc gaagtccctg
     6901 atcaggttgc gggcatcgaa aagggggttt cccgaccggg atcgaccacc agaacccgca
     6961 tttcaatgcc cggaccgcga cgagcgggcg gattcgcgtt cagtgccagt cagccagcgc
     7021 cagcagcagc gactccatcg ccagcagcgg cgcaacgttg gtttccagtg ccgtgcggca
     7081 ggccagaatc gcgtccagcc ttgccaccgt ggcatcggcc gatccgcgac gggcggcgat
     7141 gccaatggcg tccgcgattt cggcgttgat cagcgccgcc ccagagccgg tctggatcgc
     7201 caggacatcg cggtagaagg cggtgagctc ggtgaggacg cggtcgagcg cgtcgcgctg
     7261 cagtcgcttg gcgcgggcct tctgctgatc ctccagctcc cgcaacgccg cctgcgcgtt
     7321 ccgcggccga gcgccacgag tgccgaaccc cagcgcttcg ctcagttcgg ccttctcgcg
     7381 tgcgtccagc tcggacgtga ccgcggcagc ctcctcggtc gcggcgtcga ccatgctcgc
     7441 cgccgcgctc aggcaggcgc caagggaggt cagccgaccc ggcagcgcca gcacatcggc
     7501 tcggcgtgcg cgggcggtct cgctgcgggc cagcgcgcgg gcgcgtccga tatggccctg
     7561 ggcgacccgc gctgcgaaac cagccagttg cggctcgacg ccgtccctcc gctgcagcag
     7621 ttcggcgatg gcagcggtgc tcggagtgct cagttccatc aggcgacagc gggagcggat
     7681 cgtcgccacg acatcgttcg acgtgggtgc gcacagcagc cagacggttc ggggagccgg
     7741 ctcctcgatg ctcttcagca gggcgtccgc gccccgctcg gtgatgcggt ccgcgtcctc
     7801 gaccaccagc acctggtaac ggcccagcgt cggcgccatc gccgagcgtc gtaccaggtc
     7861 gcgcacctca tcgacaccga tgctcagctg ctcggtgcgc acgagggtga catcggggtg
     7921 cgcaccgctg agcgccgtca ggcaggtatt gcagtcaccg cagccaccga actggcactg
     7981 cagcgcagcg gcgaaggctc tggctgcgtt cgagcggccc gagcccggcg gaccggtgaa
     8041 cagccaggcg tgggtcatgg cgtgccggtt gccgtccgcg ccggcgccac ggacggcgcg
     8101 ttgcagcact tcgaccacgc gatcctggcc gatcagctcg gtccagaccc cgtcggcgaa
     8161 agtgctcgtc gagttcacgg cgtcagcttg ccagacggca ccgacaacag ctcgcccacc
     8221 cgctcgcgca cggcggcagc gatgctttcg atgcccgttc tggcgggcag caccagatag
     8281 tgctccgggt cccgagcggc gagatccagg aagaactgcc gcgcccggac gtgcaggtcg
     8341 gtgccggcac cttcgaggcg atccttctcg gcgatcgtcg cgagcccgtg gtcggggtcg
     8401 agatcgagca ggacggtgag gtcgggcagc aggtcttcgg tggcccaacg cgccacctgc
     8461 tcgacccgct cgacggacag cacccgaccc gcgccctggt aggccagcat cgagtcgaca
     8521 taacggtcgc tgatcaccac tgcaccacgg cccagcgccg gcttgaccac ctcgtagacg
     8581 tgttgcgcct tgtcagcggc gtacatcagg gcttcggccc gcggactgac atcaccccag
     8641 gacgggttca gcaccaggtc ccgcagtacc cgcccgatcg ccgtcccgcc cggctcaagg
     8701 gtgaccacga cttcgcgtcc ctgcccggcc gcccactcgg ccagcagcgt cgcctgggtc
     8761 gacttgccca cgccgtcacc gccctcgaag acgatgaaca ctccctcgcc catcgtgtct
     8821 cctctccccc cgtccgcctt ccggaaactt cgctcccgaa agggacccgc gctcccgaat
     8881 attcgggagc tagcgtccct ttcgggagcg aacttgccag ggagcgggcg ggctacttgg
     8941 ccggcttggt ggtggtggtg gtcttcttgg tggccggctt ctttgtggtc gtggccttct
     9001 tggtggtcgt ggtgcggcgt gcggtggtgc gcttcggcgc cggtcccttt gcgcgcttct
     9061 cggccagcag ttcggcggcc cgctccagcg tcatggcctc gaccgaatcg gtgcggcgca
     9121 gggtcgcgtt gaactcgccg tcggtgacat acggaccaaa tcggccgtcc ttgaccacca
     9181 ccggcttgcc gctaaccggg tcggcaccca gttcgcgcaa cggcccggac gccgcggccg
     9241 cccgtccacg ggtcttcggc tgggcgtaga tcgcctccgc atcagcgagg gtgatctcga
     9301 agatctggtc ttcactggtc agcgagcgcg agtccgtccc cttcttcagg tagggaccgt
     9361 agcgcccgtt ctgcgcggtg atctcctcac cgtccgcggc cttgcccacc aggcgcggca
     9421 ggctgagcaa ctgcagcgcc tgctccaacg tcacagtctc ggtgctcatg gacgcgaaca
     9481 gcgatccggt ccgcggcttc gctgacttcg gcgcgtcctc gggaagtact tcagtgacgt
     9541 acggaccgaa gcgtccgtcc ttcgcgacga tcgcgttacc cgaggccggg tcgatgccga
     9601 tcgtccgctc ctcgatggcc ggcttggcca gcagttcacg ggcgaagtcg agcgtcagtg
     9661 cgtccggcgc caggtcggga tcgacgttgg ctcgatgccc ctcggaatcc tcgacatagg
     9721 tgccgtagcg tccgacccgc acgtccacat cgtcgccgag gttgaaggta gacagcttac
     9781 gcgcgtcgat ctcgccgagt tcggtgacca gcttctccag accctcgtgg gccgagcctt
     9841 cagggccgaa gtagaagtcc ttgagcaccg cgacccgggc ggcatcgccg gcggcgatct
     9901 cgtcgagggt ctgttccatc tctgcggtga acgcgtagtc gaccagcttc gcgaagtgct
     9961 cctccagcag ccgcgtgacc gcgaacgcca gccaggtcgg caccagcgcc gaacccttct
    10021 tgaacacgta gtcccgcgac gtgatcgtgc ggatgatcgc cgcatacgtc gagggacgcc
    10081 cgatgttcaa ctcctccagc ttcgccacca gggacggttc ggtgtagcgg gccggcggct
    10141 tggtctcgtg ggcttgcgcg ctgatcgtct ccgcttccag gcgctgcccg gacgcgagtt
    10201 gcggcagccg ggcctgcgcg tcgtcggtgg acgaatcgtc gtcgaccgac tcgacgtagg
    10261 ccttcaggaa gccaaggaag gtgatcgtgc gaccggacgc gttgaaggtc gcgcggcccg
    10321 aatcgaccac ctcgccggtg gccgcgtcgg tcactgacac acgctcgggc agggtcgcgc
    10381 cgatgcggac gctgaccgtc tcgccctcgg cgtccttcat ctgggacgcg atcgtgcgct
    10441 tccaaatcaa ctcgtagagc cggaactggt caccgtgcag gccggtctgc tgcggcgtgc
    10501 ggaacgtcgc gccagcgggg cgaatggctt cgtgcgcctc ctgcgcgctc ttcaccttgg
    10561 acgcgtacac ccgcggctgc gcgggcaggt acttgccgcc gtacagctcc ttgacctggg
    10621 cgcgcgcggc ttcgatagca gtgctcgaga gcgtcaccga gtcggttcgc atataggtga
    10681 tgaagccgcg ttcgtagagt tcctgggcca ccgacatcgt gcgctgcgcg gtgaagccga
    10741 gtttgcggcc agcttcctgc tgcagggtgg tggtgcggaa gggcgcgtac ggcttgcggg
    10801 tgtagccctt cgcctccacg ctgccgaccg agaaaccggc cttggcaagg tcggacgcga
    10861 ggcgctgcgc cgcatcgatg ccgagctgca gcaggtcctt gccggcgagc ttgccgaccg
    10921 agtcgaagtc gcggccctgg gcgatccggc gcgatccgaa tgcggtgagc ttggcggtga
    10981 aggtgcgcgg atcagcgtcc acaccggcgt ccaggaccgc ctcgatatcg gcgtaggcgg
    11041 cagcgcggaa cgcaatccgt tcgcgctcgc gatcaacgac cagacgggtc gccaccgact
    11101 gcacgcggcc cgccgacagc ttcggcatga ccttcttcca gagcaccggc gagacttcgt
    11161 agccgtaaag ccggtcgagg atgcgccggg tctcctgcgc gtccaccagg tcggtgtcga
    11221 gctcgcgggt gttctcgact gcggcgtgga tcgcctcggg cgtgatctcg tggaacacca
    11281 tccgcttgac cggcaccttc ggcttcagct cgtcgagcag gtgccaggcg atcgcctcgc
    11341 cctcgcggtc ctcatcggtg gcgagcagga gttcgtccgc gttcttcagc ttggccttga
    11401 ggtcgcggat cgtgcccttc ttgtcggccg cgacgacgta cagcggctcg aagtcggcgg
    11461 cgatgttgac gccggtgcgg gcccacttct cgcccttgta cttcgccggc acctcggcgg
    11521 caccggtcgg caggtcgcgg acgtggccgc ggctggactc gaccacgaag cccgacccga
    11581 ggaacttggc caggctgacc gccttggtcg gggactcgac gatgacgagc ctggacttgt
    11641 gctcagttgc cacttggctt cttcctggcg ttcggggaca cgatcgatcg ctcactctag
    11701 ttcttcgcca cggatgctgg tgatgtgtcc ctcaactggc gagtcagttg tcggaggcac
    11761 cactggccaa ccgtggaatc ggtagggccg caccagaccg gtgccctggc cagccgagcc
    11821 acgagatggc agatcaggtc gtactgagcg tggccggcga cgccaggcgc ccggatggag
    11881 tcgcgcagcg gcctcgggtt tgccggcacc gaatcgcggc gaacccgact tggatggcat
    11941 ctcggcgacc tggcttcgta gcgagcgagc ctgacccgac ttggatggca tatcgagcct
    12001 caccagcagc ccataagcca tcgaaaccgg gtttcgcctc gaacagccgg acagacccgc
    12061 cccaaatgcc atcaaagtcg ggttcaaccg cgcgaagcca accaggccgc gccgatcagg
    12121 tgccgctcgc gacgacagcc atcggtggat ccgggctgaa ccgggctaac ccggatccga
    12181 tctggtcgca actgctcagt tgactggact gctgcccagg gtgaccgtcg cctccttctg
    12241 cgagccgttc gaagccgtcc actcgatgct cacctgctgg cccggttcga gcgcccggat
    12301 cactgtggcc aggttggtgc tggccttgat cgtggtgtcg cccacccggg tcagggtgct
    12361 gccggccttg accccggcct tcgcggccgg gctgtccgcc accacttcgg tgatggtctt
    12421 gccggcggag cgctggtcgg cgtccgccac cttgatgccc aggaagccgg ccgggccgac
    12481 gcggacggtg ccagcgtcct tcccggattc gacctgctcc accacggcca gggcggtggc
    12541 gatcgggacg gcgtaactgg tgccctcgcg agtggagccg gcggtggtca tgccgagcac
    12601 ctcgttctcg tcgtcgaaca tcgggccgcc ggaatcgccc gggacggcac cggcgtcggt
    12661 ctcgatgagg ccggtgaggt cctcagtgtt gccccacggc gagtcggacg agacggtgag
    12721 gtcctgatct gtgccggtga cctcgccggg ggccttgacc aactctcccc caccgttggc
    12781 gttgccgact gcggccacgg catcgccgac gtcaacgggg tcgtcgtcga tcgtgacggt
    12841 gttcagcccg ctggcgtcct tcaactgcag cagggccacg tccttggtct ggtcgaagcc
    12901 gacgacggtg gcggagtagg tgtttccgct cactgcgtcg gtgacagcca gcttcgtcga
    12961 tccggcgacc acgtggtaat tggtgagcac ccggccgtcc gcagtgagaa tcatgccggt
    13021 gcccgcggcg agcccggagg cggtttcggc ttcgatcagc accacgccgg ctgcctgccc
    13081 gtcgctgacg ccgttgctgg tcgccgagcc gccggtctcc tcttcgggct gggtcggctg
    13141 gatctgggca gggtcctcgg acggttcatc gcgcgggtag tacggctcga tcgttggctg
    13201 cgtggtgcgg ctcgatgacg gcagcgcacc atcgaactgg cgggccgccc agaacgaacc
    13261 ggacgcgatc agcaggaatg ccgccagcat cgcaagcact gcgatcggcc agcgcttctt
    13321 cgtcggcgtg gtggtgtgct gcggctggta gtagccgttc ggcccgtagg gactctgtcc
    13381 gtacgctgac tgagccggac gttgggcgtc ctgctggtag cccgggccgt actgggcatg
    13441 gcccggttgc tgaccgtaag cctgctggcc gtaagcctgc tggccgtaag cctgctggcc
    13501 gtgaggctgc tggccgtggg gctgctgacc gtaaacctgc tggccgtagg gctgctggga
    13561 gtactgctgc ccgtagtagc cgggctggta gtaacccgcc tgctgctggc cagctcccgg
    13621 acggaagtag ggctgctgtc cgtacgcctg gccgggatac tgctgcgcgg tcgggtactg
    13681 gccgccgccg ggcgtggtgg ggatcggtgt ggtcggaccg gtcgccgcac tcgggatccg
    13741 ggtggttgga ttggccgctg cgggcgcatt cggggtggtc gcatcgaagc gcttggttgc
    13801 gtccggcgcc gcccaattgc cactcaggtc cgccgggcgc gcccaagggg atgtcggcgc
    13861 cgaagcagcc ggcagcgggg gcaccgggac actcgggagc acctgggtgg gctcggtcgc
    13921 gtccgcaccc tgcggggcat cgggtcgaat atcgtcaagc tctgccatcg tcttggactc
    13981 cgttctcttc tgacctccag tgaacgcggt cactcaatgg ggaagctgta ccaatcctgt
    14041 gagaacctgt gaaggatcgc cggcgctcac gcaactacga agccatcggc caccagccgc
    14101 cgcagttgcg gtagtacttc cgcggccagc tctacggtat cggtgtcgag aaggcccgcg
    14161 accgcgccga tcaggacgcc gagtggcaga tcaccatcgc acgcgcccac gatcgcggcg
    14221 agcgctgtgc cggcctcgac cgcgcgtccg aagccgaatt gctggcgcag cacgatgtgc
    14281 tgggggtgcg ctgcgccggg catcccaatg gtctcctgga ccacgccggg gtgcagccgc
    14341 agcgaggtgg cgagcagttc ggcgtccgtc agttgggtgg tcggaatcgc cgtgaagaag
    14401 tcactgaagg cttgtccgac cggctgatgg acggcgtgcg gccattcctc gatccgcacg
    14461 accggcgccg gacacttcga cttggccaag ccgatccagc ccatgccaac gcccacgatg
    14521 cgcagactca cgaagtagtc cagccattcg gcgtagcggg acgcatactc gtcggtgcct
    14581 tggaggccgg cgtcggtgag ccagatctcg atgtactcgt aggggtcgag cagttcgcgc
    14641 tgcagcacga gggcatcgca gccgtccggt atccactcct tgaggtgctg ctcccactgc
    14701 tcgggatcag tgaccgccca gttacccagc acgatcagca gtccgtcgtc ggtgaggtgg
    14761 tcggcggcct gagcgaccac ggttcgcatc agtccgtcgc cgctgaatcc gccctcgcgg
    14821 tagaccagct tggcctcgct cggcgggctg agcacgtagg gcgggttggt cacgatcagg
    14881 tcgaaccgct caccggcgac cggctcgtag agcgagccga ggcgcagctc ggcctcgacg
    14941 ttcgagagac cgagtccgat cctggcgagt tcgagggccc gcgggttcag gtcggtggcg
    15001 acgatctcgg ctgcgtccag gtgcagcgcc tggataccgc agccggtgcc gaggtcgagc
    15061 accctcgggt agcggcgcgg cggcacgagt tgggtgagcg tggtggacgc cggcgaggca
    15121 cccaggacga aatctggacg cggctgcgga acccgttcat cgagcggggt gtggtcggag
    15181 cagatccagc cctcgcggcg gtcggagccg tgtggcttga gttcgatggt ggcgcgaagc
    15241 tctgcgtctc cgacgaccag cccagctgcc ttcagcgcgg gcaggctggt ccagttcgcc
    15301 agtcgctgcg ccggaacggg tagctgcagc agccagagcc ggatcgcgtc cgcttggggg
    15361 tccgtcgcgt cgccgagcgc ccgggcggcg gcgaccgaag tgttgcgggc cagcgcggcg
    15421 attgccgctg agcccagacg ctccgtcagc ccgtcaaggg tgtagtcggc ggcgagcacg
    15481 tcggcgcgca gctgggcgat ggcggagtcg gtgagcaccg gtcaagcttg ccagcaatcg
    15541 ctctgcacac agcgccgcgg gatcgtggac gcaacggtgg ctcaggccag gtcgactttt
    15601 gccaactgca cgccggcgca ggtcggccct aggaaagtgg agttgccgag atgcgggagg
    15661 agccatgcgg attgtcttgc tcggggcgtc gggcaatacc gggcgcgaga tcacccgact
    15721 gctgtcgccg atgctcgccg aggatgacca ggtggtgctg gctggacgcg atcgcggacg
    15781 gctggaggcc accgccgaag tggtgagcgg gccggccgaa gtggaactca ggcaggtgga
    15841 cgcaaccgtc gacagtgccg tacgggacct ggtcgagggc gcgcagcagg tggtggtgac
    15901 tgccagtctt cccgatcggg tcggcgcgct ggcacagatc gtggcggacg cgggcgcgga
    15961 ctggatcgac accctgctct cctcgccggc caagctggcc gcgctacgtg ccctggagcc
    16021 ggtgctgcgc gcccgcgggc ggtgcttcgt gactgacggc ggcttccatc cggggctgcc
    16081 ggcggcgatg gtgcggtggg ccggtgacca ggtcgatgag ctgatcaccg ccgacgtgtt
    16141 cggtgggatg cggatcgact ggcgggccga cagcctggcc gagtcgaccg tggccgagat
    16201 gctgaccgag ttcggcgact tcgatctgag cacctgggtg gacggtggca cccggaagct
    16261 gcggatgtcg gagtgcccga aggtggactt cggcccgccc acagaccgag agtgatctgg
    16321 gacctcccgc aggctgaccg cctgcggcct cggtgcacct cccccaacgg ctcctgagct
    16381 ccactgattc ggctggtcaa gcaggtcgag acccacccga ttgcagccgg ggaatacttg
    16441 cgtggcaggc tgtccgcgtg gcagttccgg cttggcttct agacgatccg cggctgcgcc
    16501 accaccatca ccgcgcggcg gcgcacggca gcaccgctga ctttcccgat tggctcagcg
    16561 aacggctgcg ggagccgctg tcggcacagg gcatcactcg actctgggag caccaggccg
    16621 tcgcagcgga agccgctttc gccggcaggc atgtggcgat ggccaccggc acggcttccg
    16681 gaaagacgct cggctacctg ctgccggtgc tcgccgctac cgcggacggc acggtcggtt
    16741 ttgacatcga ccacggacca ctagatcttg gtcgccggca cagcgccatc tacctggcac
    16801 ccaccaaagc cctggcccac gaccaggccc gggtgtgccg ctcactggcc ctgcccgggt
    16861 ggcgcaccac cacccttgac ggcgactcca gtaccgagga acgacggttc gcccgcgatt
    16921 tcggccgtta tgtgttgacc aaccccgaca tgctgcaccg ctcggtactg ccccgacacc
    16981 aacagtggac gcggctactg tccaccgtgc gctacgtcgt gatcgacgag gcccaccgtt
    17041 atcgcggcgt cttcggcgca catgtcgccg cggtgctgcg ccggcttcgg cggctggcca
    17101 accactacgg cgccgacccc gtgttcgtct cggcgtcagc aaccgtcgcg gacgcgggcg
    17161 gtctgctctc gctgctcgcc ggcgtggatg acgtcctcga agtcgccgcg gacacgtccc
    17221 ggcgggccgc gctcgactac ctgatctggc aaccggaggc tgaccctcac cacgaggctg
    17281 ccgacatgct cgctcgactc gtgagcgaag gtcgccagac tcttgccttc accacctcgc
    17341 gggtgcaagc cgaactggtc gcgcttcgtg cccaacgcaa aggcggcgac gactccgaca
    17401 tccagccgta tcgcggcggc tacctggccg aagatcgccg cgaactcgag gccgggctgc
    17461 agtcgggccg cctccgcggg gtggcctgca ccaacgcgtt ggaactcggc gtcgacatct
    17521 ccggcgtgga cgcggtgctc acctgcggct tcccgggaac ccgcgccgcg ttgtggcagc
    17581 aggccggccg ggccgggcga gccggacgcg acgcgctcgc ggtcctgatc gcgcgtccgg
    17641 acccactgga cgcgttcttc tgtgagcatc ccgaagccat cttcgacgct ccggtcgagc
    17701 ggacggtgct tcaccccgag aacccgaatg tgctggctcc ccacatcgcc gcggctgcag
    17761 ccgagttgcc ggtcacgctg gccgaccgga aatggttcgg cgaaaccctg cccgccctgc
    17821 tggacgggtt gaccgcggcc gggcacctgc gcaagcgtcc gggcggttgg ttctggacgc
    17881 gctctgatcg tccagcggac gcgattgacc tgcgctcgat cggcggtgcc agcgtcgagg
    17941 tggtcgagac tgtgactggt cgcgtgatcg gccaagtcga tccagccgcg gcagaccgga
    18001 ccgtgcacac cggcgcggtc tacctgcatc agggtgacca gtggcgggtg ctcgagtacg
    18061 acccggctgg tcacaccgct ctggcgcaac gggtgcagga gggctacttc acccaggccc
    18121 tcggcgccag cgacgttcgg attctcggtg ttcagcagga gcgggtcctc ggtgccggca
    18181 gggtgtactt cggcgcagtg gaactctcca gccaggtgac cggcttcctg cgtcgggacg
    18241 aggcttccgg aaaggtctgg gactcgcacc ccctcgacct gccccaacga accctgcgca
    18301 cccaggcggt ctggtacacc gtcccccccg ctgcgctcgg cggcattccc gtccgcgatc
    18361 ttcccggtgc ggcgcacgct gccgagcaca ccgcgatcgg cctgctgccg gcgttcgcgc
    18421 cgtgcgatcg ctgggacatc ggcgggctgt cgaccgccct gcatgccgac accggccagc
    18481 tcaccgtgtt cgttcacgac ggccaccccg gtggcgccgg tttcgcccag cgcggcttcg
    18541 aatcggtcga ggcttggctc accgcgaccc gggatcggct cttgcagtgc ccctgcgccg
    18601 ccggatgccc agcctgtgtg gtctcgccga aatgtggcaa cggaaaccag ccactggaga
    18661 aggctgcggc ggccagcctg cttgccctcc tgatcacctg atcgcggcca cctcggtggc
    18721 acatccggta cccgagccac ctcggtcacc ccagcccggt gcgcccggct cgcgccgtgg
    18781 cttgcacccg ctgcggcagc accggcgctc caaaccgtac ggaacgcgca accgtgacca
    18841 cgaccacgaa gtccaaggtg tcgccgacta ctcgacagcc agtcaccgcc acttggttgg
    18901 cgctagccgt gctccgagcg gctcggcacg cgtcggctcc ccgaacgcgc tcggcagcgg
    18961 ccgacagtgc caccaggtcg gcggcaccgc gagcctcatt cagggcggcc aggtagccga
    19021 tcaccacggc cagagcgccc acaacgacca agagcacaag ggaaaccgcc gccatctgcg
    19081 cggtgccgga gccgcgctcc acctcaggtg gacgaaaacc acgccttcgc tcggcgcgac
    19141 ggacgcggga tgcgcggcgc ttcatgccgg ctcccgaatg accgtcgcgg tggcggacac
    19201 cggaatcgcg ggtaaccagg ccgcccatgg ccgagcatcg agacgtacct ccacccgcac
    19261 ctcgttcccg tggttgccga ccaacacctg ggccccggtc ggccggtcgg cgatcgcctt
    19321 ggccacagcg ctccgatcgg cccgcgcctc ctgccgggcc acttcagccg cagtgtcctg
    19381 gcagcgcacc agcagggcca gcaccaccag cacccacgcc agccccacag tcgcgagagc
    19441 cgccccaatg ctggcgaaag ctatctcagc ggtgaccatt ccccgctgcc ggtcgcgctg
    19501 gcccagcgat gccttccagt tccacgaacc acagacccgc ttgcggtagt tcatccgtcc
    19561 cttcctcttt taggcgccta ttcgccgtac ggccaggagg ctgctgaacc tcgcggctcc
    19621 gagcaccagg tgccgatcgc acgcgaggct cagcagtccc tgaccaccga ccgcttcgcc
    19681 agatccaccg tctgcacatg gcgattccgt aaaggccccg gatgacggcg ggctcggcag
    19741 aggcgaccgg cgccggttca gctgccgctg atggcctgca ggatgaccgc gatgatcgcc
    19801 ttgaggatct gccagatgat ctgctggaaa tccgggttgt tcaggattcc gatgatcacc
    19861 cccaccacgc tcaccgtggc gaccgtgccc accgcgtact ccgcggtagt catgccgcgc
    19921 tcgttgtgcc gacgcaccaa ctgacctgcc attccttctc cttccgtccg cgtccaaccg
    19981 gacgcgtcca taccttcggc aagtcggtcg ggattcggtc acccaacttc ttgggctgtg
    20041 gacaacactc cgggcacccc gaaaactgtg gaaaccagcg tccactcatc atctgctcgg
    20101 ccatgttgag accgggggca cggatctaga cacgctcggt cactcggcgg tcgagcatgg
    20161 gcggcgggcg cagctcatca gccgaacaga tgtgcggcca cgccaccgat gatcggcacc
    20221 acaccgagca gcaggaacgc cggcaggaaa cacaccatca gcggcaggac gctgcgcaca
    20281 cccactcgct tggcacgcac ctctttggca gccagtgact cgcgtcgggc gtccgccccg
    20341 agcgcacgca tggtcttggc caacgtcgtg cccgatacga cgctgcgagc aacttcacgg
    20401 cagagcccgg ccagggccgg ctccgccgca gcggcgtccg cccatgccgc gccttcgtcg
    20461 gtgccgaggc cgacctgacc accgatttgg cccagcacgc cccgaagtgg ctcgtccacc
    20521 gcgtcaccca cgaccgccac cgcagcccgc aacggaagtc cggcttccaa acagaccgcc
    20581 agcaggtcac agacctgcgg cagtccggcg accaactcgg cgttgcggcg aaccgtgttg
    20641 ctggtttcca gccgaccaag caccagataa ctcgcgaccc cgatcgcggc cgccgccggc
    20701 agagcccacc agccgagccc cgccgtcaac agcagcgcac tcaccgcagt gccgaacgcc
    20761 atcccgagcc gctgactggg ttggatgagc ctccgccttc cagtcggcgc aaccaacccc
    20821 gccgccagtc gagcggacgg gctgccccag gccaacaccg ccagcgccac cgaaagcgca
    20881 gccagccacg tcccgctcaa ctcaggtcct cttccgagcc ggcaattcgc tcggtccaga
    20941 acacgcccac gcagccgagc agggcaccta aggccaagca gagttgcccc ggcaccgacg
    21001 cggttaggaa ctccagcgga tcaccgccga acccgtagcc aagtccaagt cctaccaacg
    21061 gcaacacggc gagcagccgc ccggtggctc gtggaccgga cagttcggca gccactacgt
    21121 tgtgcaccgc ccgatctgcg gcgagccgct cagccagggc gtccaaagtg gcggtcaggg
    21181 acgcaccagt tcgctcggcc acatcccaag cgattcccag ctgcgccagg ccgccgtagc
    21241 ccccgacggc gctctgcctc cgcagggcgg cggccacctc cccaccgatc gcgtggatag
    21301 cggcggcgtc cgccagcacc ggtgcgtcct gcgcggccaa cctcagagcc accccgggga
    21361 cgtgacccac tcgcagcaat ccggccagtt gctggcaggc ggtggcgacg tcccgagccg
    21421 tcgcggctgc ggttgaacgc cgccggtgcc gccgccaaac cgtccaggcc gtcaccagtg
    21481 gaagcgtcac tgcgaaggcg accgatccgc cggcgccacc ccagaccatc aggccgatga
    21541 tgctcgccac agcaaccacc accaagggaa acgccgcacc aagccgccgc tgggagcttg
    21601 gcgaccaggc cgcaccgcct tctaccggac gcaacctcgt caccgccgat ggcgcgaacc
    21661 ccacccaacc ggcgaggaac gcggccagca ccgcgatcgc ggtcatgacg ccgccagcgc
    21721 catcagctcg tcgaacccgg gaccgggcac ggcaccctcg gctgagaacc gcaccgccgg
    21781 aacggttcgg cagcggccat ccggggacgg ctcgacgagt tggatctcgt ccacccagcg
    21841 tcgcccggcg ggcagccggc gcacatggat cagcacccgg agcgcggccc cgagttgagc
    21901 gtgcaacgca tcccgttcga ggccaccgag tgcgccgagc gcctccagcc gggcggggac
    21961 gtccgcggcc gagttggcgt gcacggtgcc acagccaccc tcgtggccgg tgttcatcgc
    22021 gcgcagcagg tcggcgagtt caccgccgcg cacctcgccc acgacgatcc gatcggggcg
    22081 catccgtaac gcctgccgaa ccaggtcggt gagggtgacg gcaccagcac cctcagcgtt
    22141 gggcggacgc gcctccagcc ggacgcagtg tgggtgttca ggctggagtt cgcgagagtc
    22201 ctcgaccacc acgatccgct cgtctggcgg cacctgggca agcatcgccc ctagcagagt
    22261 ggtttttccc gaaccagtgc cgccggagat caggaacgcc cgcctagctg ccaccagccc
    22321 ggccagcagc ttcgcggcat ccgcagagac gctgcccgac gcgacccaat cgtgcagccc
    22381 caatcgctgt cgattgggca cccgcagcga gaggcaggtg ccggtgtcgg caaggcaggg
    22441 cagcaccgcg tgcacccgga ctccgttggg caaccgggca tccacgaatg gtgagccgtc
    22501 gtcgagccgt ctcccgacgg aggcggccag ccgcaccgcc aatcgccgca gttccgcctc
    22561 gctggcaaag cggacgccag ccggctcaag cccgccgcca cggtcgaggt agacgtgatc
    22621 gggaccgttc accagcacgt cggtcacacc gggaagcgcc aacagcggct ccaaaacccc
    22681 agcccccaca ctttcccgac gtaacgcagc cacggttgaa cgcaccgcag agtcgctcac
    22741 tagcatgccc agactccgca acgcggcgcc gacctcaacc gggccaggct gctgtccgag
    22801 ccggccaagg tgggtccgca cggcgtccag gtcgggttca ggcatgaccg ggttcctcgc
    22861 tccgcacggc cgtcagcact cgggcaaccg aagcccggta gcggcgccgc ccgcctcgtg
    22921 ccggcggctc ccccagttcg gcggcggcaa ccaggcgcgg ttcctcgggc agttcggcca
    22981 gcacgggcag cccgatcgcg tcggcgacca cgtcggcggc caacccacct cttcggcgtc
    23041 gaagcaccag ctcggcgccg tccaacccca gcacctcgac cgcagccctg gcagcggcca
    23101 ccccgcgcac tccggcggac accaccaata gcacccgacc cagccggacc gcctcacggc
    23161 aggccggccc aagtgatcta cccgcgtcga gaaccaccag cgagtgccag gtgcgcagcg
    23221 atccgacgat cgcggccaac ggctcgcggg caagatccag agtggccgtg cgcgccatcg
    23281 acaccaccga aacctgctcc accaccggca ggtacggacg caggtcgccc acctgaccgt
    23341 ccgcaccgtt gagtcgtggc cagcgccagc cgtcggtgcg ctccgcgccc agcagcaggt
    23401 cgatgccgcc gccgagtggg tctacgtcca ccaatgcagc gccgccgccg gagtctgcag
    23461 ccaactgcgc cagcgccgca gccagggtcg aggcgcctac gccgcctgag gcgcccagca
    23521 cggtcaccac cggagcccga tcacggctgg tgcgatcgga cagcactgca cccagccagg
    23581 cgccaccgtc aggcagctga atcaccctgg ccgacagcgg tgccgaccat ctggataact
    23641 ccgacgcatc cgacccgacc aggaacaccc cgtcgcgtct cggcagtgcg cgggcggcca
    23701 ggcgcgctgc ctggtcactc gcgaccaaca ccgtgccggc cgcccgccat ggctgcccga
    23761 tccgctcgag gtcgctcacc acctcggcgt ccagcgcaag ggccgcgacc gcagaaagga
    23821 tcacgtcctg ggtctccccg tccccagcga ccaccagtac gtcacggcca ctactcgcct
    23881 cagccacgtc cacaccttcg gcaaccacac cggaattccg tcagcccaac cgtccgcctg
    23941 tggataacca ccgctggccg ggagttgtcc acaagtcgag gagccccgga gattcgaacg
    24001 gccgccgagc ccttggtcga cctacgattc ccccatggca actcccggag cgttccccgc
    24061 agccgcggtt gcctggctcg cgcccacgcg tccgggcagc ctgctggcga tcggatcagc
    24121 ctccgcgccc accgccacgc acttcgagca gctggggaac cgcgtcacca tcactgcacg
    24181 cgcccctgaa gacgtccgcc gtgcctgtgc tcgccacacc ggcttccacg gcgtcgcggc
    24241 agcaccggac gcactaccct tcctgccgtg ctccttcgac gcggtgctcg tggcacaggg
    24301 catgcaccga ttgcccccgg gcctggtact cgctgagttc gcccgcgtgc tcgtccctgg
    24361 cgggcaactc gcggtggtgg ccaccttccg cgacgactcg gtgccctggg tgcgtcgctt
    24421 ggcggccatt ctgcagcgct acgaccccga tgcgatgcgc ggcgccgacg agtctgccgc
    24481 gaccattccc gcgtccgcgc atttccccga ggtcgagcag cgtgacttcc ggcggtgggt
    24541 gccgatcacg cgcgacggga tgctgaagct ggtttcgtct gcaccgaagc tggcgaacct
    24601 cgacatcggc gtcgccgaac ccctgttggc cgaggtcgcc gagctgtacg actcgtcggc
    24661 tccacgcgcc gaacctctgc tgctgcccta cgccgtccgc tgctcacgag ccgaggtcga
    24721 ccacgccgag ctgagcagcg tgctccgcct ccccgaggac ggactgcaaa tcaacctgca
    24781 gtcccgcccg tttgtcagtg atagcgatta ggctgaccac aaatcggggc cgaaggagcg
    24841 tggaatggcc gatcagtccg gaatcgccga cgtgatcaag agcatccagg acgatgtgcg
    24901 caccatcgtc aagggcgaac tcgagctggc caaggccgag ttgctgcccc aagcgaagtc
    24961 ggccggaatc ggcgctggcc tcttcggggg ggccgcctat ctgggcatca tgggcgcgac
    25021 tctgctgttc tgcgggctct ccttctggct gtcgttcggc ttccagagtt ggttcggcat
    25081 cgacctgttg gccgcgctgg cttggggttt cgctgtcatg gcgatattga tgttcctgct
    25141 ggccggcgtt ctttcgctga tcggcaagca gcgtctcaac ctgcccggcc cgcaggccac
    25201 gactgccagc gcgcaagcgt cggtggacgc ggtcaagggc gccatcacca agggccagga
    25261 gcgggtggcc gcgacgtcca tcctcggcgg ggagccgcgc aaagagcttg agtgagcgga
    25321 cgcgggcctc gacgggctct accagccggg agcccgtttg agacggtgag cgaacgatcc
    25381 ttcgacaagc tcaccacctg atccacctat cccgctgaga gatcccggcc tccgccggga
    25441 tgacgaaggg annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nggatgacga
    25561 agggacgccg ggacgacggt gggatcgggc tccggagggt ggttacaggc gctcgctcag
    25621 gaagcgtggc ggaatctcgg tgaccaggtc ggtagcccag ggctgcgtcc agcccagcag
    25681 gtcaaacagc ccgtccaaca ggtaggcggt gaaaccccac acgtacagat cgtcgagagt
    25741 gaacgccggc ccgcggaagc cacccgggtg cgcccacgtc gatcggttcg ccgggtcgag
    25801 cagacgggac accggcacct ggtgaacgcc cgccaactcg ccgacgtcca ccacctgcag
    25861 tcgcaccggc tcgccccacc agcccacgac ggaggtcacg tcgaacccgc tacgcgggat
    25921 gtgtgcgtcc ggcagtgaac cgagcaccac caccgcggac gggtcgatgc ccacctcctc
    25981 gttcgcctcc cggagtgcgg cggcgaccgg attgaggtcc tgcggctcca gcgatccacc
    26041 ggggaacgcg atctggccgg cgtgactacg cagatccgga cgtttctcga ccagcacgat
    26101 ctcgtaggcg ttgggctcgc cgctgagcag gatcagcacg gcagcccgcc gcttgccctg
    26161 cggtgtccgc gcaccctgaa tgaagctcag cgcgtccgga cggtcgaatt gtgcagccgc
    26221 agcccgcagc ccgtccggca cgggaaccgg gaagctcacg cggccagccc cgctgccacc
    26281 cacgactcga tctcttccag cgaggtgaag atgccgggat gccgggccac cactacgccg
    26341 tcggcatcga tcagcaccgt gtgcgggatg ccggccaccc ccagcggacc gcgaatggtc
    26401 gcctcttcgt caaccaactg ggggtacttc atcgccatca actgcgcgaa ctcgatggcc
    26461 agttctggtt gcggatcgga gtagttgata ccgagcacgg ccacgtcctc ctgcttgctg
    26521 gcaaacgcgg acaggtccgc ggcctcggcc cggcagggtt cacaccattg agcccacagg
    26581 ttgatcagca tgggtccgcg cagcccggcc agccgaacct gactgtcccc gccgaggcaa
    26641 tcgagggtga ggtcgggcag gccgcccggc accactggcg cttcactcgg cttcgggcag
    26701 tccgcaatcc cggccgcctt ccgcgcggtc accaggtcga cggtcttggg ggtggccggc
    26761 gagtagctct cgaaaggagt cgggaaccgg ttcgccgaag gcacggacgg gctgcagccg
    26821 gccagggtca gcaggagcgc cacccccagc gctagtgctc gcagcggacg agggccaagc
    26881 ggcctcgacc cctcgacagg ctcagggcag gccaagctcg accaaccgag gctagaccaa
    26941 cgaagacgct tcatccgcgc ggctcccgca ccagtttcgc ggccaccttc ggatcggtcg
    27001 gcccttcacc gaaggacggg cagtagctgg ccaccgaaca agcgccgcat gccggtcgcc
    27061 gggcgtgaca gcgccgccgg ccgtgccaga tcacccggtg gttgagccgg atccagtccg
    27121 acttcggaaa gagctcgccg acttcggcct cgatcacttc cggcttggcc gaagcggtcc
    27181 agccgaaccg ttgcgagagc cggatgaagt gggtgtcagt ggtgatgcct ggcacgccga
    27241 aaccgtcggt gagtacgacg ttcgcggtct tgcggccaac gccgggcagc ttcacgagct
    27301 cggcgagccg ccccggcacg acgccgccgt ggtcggccac cagcgcggtg ccgagccgga
    27361 tcagcgcgtc cgtcttcgcc cggaagaagc cggtgggctt gatcagttcc tccagttcgg
    27421 tgcgatcggc agacgcgagc gcggccgcgt ccgggtagcg cgcgaacaga gccggcgtga
    27481 ccgtgttcac ccggcggtcg gtcgactggg ccgagagcac cgtggccacc agcagctgca
    27541 ggggtgaggc gtgatcgagc tcgcaatgcg cgtccgggta caactcgccg agtccgcggt
    27601 tgatcgccct ggcccggcgg accagagcga gcctcgactc ggtggcggtc acgccgcctc
    27661 gaccaccagc tcgatctcga ccggcgagtc cagtggcaac acggccaccc cgacagcgct
    27721 gcgcacatgg atgccggctt cgccgaagac ctcctgcagc agcagggacg cgccgttggc
    27781 aacggccggc tgcccggtga aaccggggtc gctggccacg aagacgacca gccgcaccac
    27841 ccgcgcgatc ccgtcgacgc ccccgacgac ggacgcggcc gcggcgaccg cgttcagcgc
    27901 tgccgtccgg gccaactcag cggcccgttc cgggctgacc tgtgcaccca ccttgccggt
    27961 gtcggtcagc ttcccgtcca cgaacggcag ctggccggcg gtgagcacct gcccacccga
    28021 gatcgtggcc ggcacgtacg cggctaccgg ggcggggacg gtcggcagcg cgatgccgag
    28081 ttcggccagc ctggcggagt gactcattcg accaccggtc gcttcaggta ggccaccagg
    28141 ttctgcggat tcggtcccgg cacgacctgc accagctccc agccgtccgc accccagttg
    28201 tcgaggatct gcttggtggc gtgcaccagc accgggacgg tcacgtactc ccacttagtc
    28261 atggagtcac cctagacagc tcggccgcac aggtgcacca cccagccggg gctcgcgcga
    28321 aaacgctacg gctcagaggt cgcagcgcga atgcttcggg cagaggatcg aagtccagga
    28381 ggtgatgtct gtgcgctggc gcagcaactg gcggcgctcc cgctcggtca tgccacccca
    28441 cacgccccac tcgatgcggt tgtccaaggc ttcggcgagg cactcgttgc gcaccgggca
    28501 ggtgtagcag accgaacgca cacgtttctg gtcggcaccg tcggggaata actgatcttc
    28561 caatccacgg cacttggcct ggagggtcca gtcttcgact ccgagcggca ttgctctcac
    28621 ctttcaccgg ggtggtcact gacatcggcg acagtaagca attgctaggc gaacgagccg
    28681 tctgctgtca agaactttgg cccggctagg gaagattccg ttcgaacccg tccgccggca
    28741 gcgtccgcga gccgattggg tgccgtgtcg taccctagaa gtcatgcgca tggggaaaag
    28801 gctgtactcg ctggtcatgt tcttggtggt ggcgatcatc ggcggactgc tggcggccgg
    28861 cctggtcgtc cccatcgccg gcatggcgac cgacaccacc acgtccatgg tggagagcct
    28921 cgaccagttg ccggccgagc ttgagacccc accgcagtcc gagcgttcca ggctgttgaa
    28981 cgcggacggt tcactgctcg cctacttcta caacgagaac cgggtctaca aggaactgaa
    29041 ggacatctcg ccgatcatgc agaaagcgca ggtcgccatc gaggatcacc gcttctacga
    29101 gcatggcgcg atggacctgc agggcacctt gcgcgcgctg gtcagcacca gccagggcaa
    29161 cacccagggc ggttcgagcc tcacccagca gtacgtccgg ctggtcctgg tcgagaacgc
    29221 cgaagccaag aacgacaagg ccggcgtgat cgcagccaag gagaacactg tcgctcgcaa
    29281 gatccgcgag ctgcgttatg cgatcgccat cgaggagaag ctcagcaagg acgagatcct
    29341 gcagaactac ctcaacatcg cctactacgg cgacggcgcc tacggggtgg aagccgcggc
    29401 caagcactac ttcggggtga gcgcggccaa gctcgatctc gcgcagtcgg cgatgctggc
    29461 cggcctggtt cgcaacccca acgcgaccaa cccggtcagc cacgtcgatc tggcgatcga
    29521 gcggcgcaac aacgtcctcg accgcatgct cgagttgaag ctgatcacct cggacgaagc
    29581 ggcggcggcc aaggcagtcg agttcgacca gagcaaggtc aaggactacc tggcgggctg
    29641 cgcgaactcc aagttcccgt tcgtctgcga ctacgcctac cgcacgctgt tgaagacgcc
    29701 cagcctcggc agcaccgagg aggagcgcaa gcagctgatc taccgcggcg ggctgacgat
    29761 catgacccag atcgaccccg cggcccagaa gaaggccgag aagacgatcg ccaactatgt
    29821 gtcggcgaag gatccagtcg tctcggtgat cacgatgatg gagcccaaga ccggcctgat
    29881 ccgggcgatg gcccagaacc gttcggagtg gggcaacaat tggaagaagg gccagaccaa
    29941 ctggaactat gcagtcggcg ccgatatggg cggtgcggac ggctaccagg gtggctcaac
    30001 cttcaaggcc ttcgtcgccg cggctgccct cgaagagaac atgggcgtga acttcaccgt
    30061 ccgcgtcaag cgctcggtga actaccagaa caagatcttc ccgacctgca acggcgacct
    30121 gaaggtccgc gacgactgga cggtggacaa ccccgaatcc ggcacgatga acctcctgca
    30181 gggcaccaag tactcagtga acaactatta cgtagcgctg gaacaggccg tcggcctttg
    30241 cgcggtcacc aacatggcca agcgcctcgg cctgaaggcc tctgatggtc gggacttcgt
    30301 cgagaccgag caggtgccgt ccttcaccct cggcatcgtc gaggtgacgc cgctgtctat
    30361 ggtcgaggcc tacggcactt tcgcgaaccg tggcgtgcac tgcaacccga tcatcctcaa
    30421 gtcgatcaac ggcaaggacg gcgccgcctt cgatgtgccg acagcgggct gcaagcgcgt
    30481 gctgagcgaa gaggtggcgg acgccgtgaa caaggttttc caggggccct acaccccggg
    30541 cggcaccgcg tacaccgcca aggtgcccgg ctacacgatg gccggcaaga ccggaaccgt
    30601 gccgaagaac aaggcagtct ggacggttgg ctacacgccc gacctggtcg gcgcggcggt
    30661 gatcagctac gacaacaaca agaactaccg gaagttctgg aaaggcaagc cgagcttcct
    30721 gcgctacgtg aagctgccgg cgtccggacg gtggctgagc ggcttctccg gcaacaacgc
    30781 cggggccaac ctgctgaagc ctgcgatgac ggcggcgctc gctgacatcg aggaacgcac
    30841 ctcgttcgtc gaaccgccgg ccgaggtgct ccgcggacaa acggtcggta tcccgagttg
    30901 cgccgggatg ggcgccgctg gttgtgccgc caccttgagg cgggccggct tcagctcctt
    30961 tacctacacg gtgaagtccg acaagccgaa gggcactttc ctcggcacga atccgagtgg
    31021 tggcgcgccg aagggcagcc agatcggact gatggtaagc cagggcccga agaagaagga
    31081 agagcccaca ccggagccgt cagccagcgc gtcaccgcag gagaaggtga aggagcaacc
    31141 gcgcaggtga tcggcaaggt gatcggcggt ctggccgccg gtgcggcggt gtgcgtcggc
    31201 tatggcgtgc tgtgcgaggc gaacgccttc cgggtgcggc gggttcgcgt cccggtgctg
    31261 ccgacgggtg cgccgaagat ccgggtgctg cacctctcgg acatccactt ggcacgagga
    31321 cagcggaaaa agctggcgtt cgtgtcgcgg ttggctgctt tggaacccga tctggtgatc
    31381 agcaccggcg acaacatcgc acacggggac gcgcttgagc cgctgctggc cgtgctgcgt
    31441 ccgctacttg aggtgcctgg ggcattcgtg ttcggttcga acgactatgt cgaggcacgg
    31501 atgtcgaacc cgctggccta tctggcgcgc tcgacgagcc acgacggcgt gcgtgaagag
    31561 ctgactccgc tgcccaccga ggctctgcgc gccgggctgg cgtccggtgg ttgggcggac
    31621 gtgaccgcgc tccgggcgac gctgcacgtc cggggagtgc aactggagtt tcgggggacg
    31681 gacgacgcgc acctgcatcg cgacgactat tccttggtgg ccggaccggc ggcttccggg
    31741 acggacctgt cgatcggggt gacccatgcg ccataccttc gggtgctgga cgggatggcc
    31801 gctgacgagg taccgctggt gttcgccggt cacacgcacg gaggccaggt gtgcgtgccc
    31861 ggttggggtg cgctgacgac gaactgcgac ctgccgccgg ccatggcgaa gggattgcac
    31921 gagtacgtgg gacgcgacgg tggccgatcc tggctgcacg tgtcggccgg tatcggcagc
    31981 tcgccgtacg caccgtaccg gttcgcatgc ccacccgagg tgaccctgtt gacgctcacg
    32041 ccggtcccga atggctgagc ggcgaatgat cggctaaact gccactcggc cctgacccag
    32101 tcagggtcgt tcggggtgtg gcgcagcttg gtagcgcgca tcgttcggga cgatgaggtc
    32161 gcaggttcaa atcctgtcac cccgaccagc ggaaagccgc tctgactagg gatt
//
LOCUS       DAOPRQ010000025        31280 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_1137_length_31280_cov_12.670905, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000025 DAOPRQ010000000
VERSION     DAOPRQ010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 31280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31280
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1137_length_31280_cov_12.670905"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(8..592)
                     /locus_tag="PKJ61_04855"
     CDS             complement(8..592)
                     /locus_tag="PKJ61_04855"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOC13186.1"
                     /translation="MTTPVSGRTDGRVARRQGTRDAIMDAATELFAARGVTSTSIDDI
                     AAAAGIAKGSIYYNFDSKSGLVEAIMIRSSERLAGALAEAKQERSGDALREAVVRALL
                     LLVQENASSARVMVTEMFRTERSWRDSIETWREIALSPLVEDSREAGVDPVVAGVQAA
                     AIVGATLMAGLEWLVFHPERSAEQVASAVLGTLR"
     gene            complement(603..3167)
                     /locus_tag="PKJ61_04860"
     CDS             complement(603..3167)
                     /locus_tag="PKJ61_04860"
                     /inference="COORDINATES: protein motif:HMM:TIGR03062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HOC13187.1"
                     /translation="MFAWTSHGTELKRFARLPITRAAVAVMLLIPLLYGAMYVWAFWD
                     PTTRMNDLPVALVNQDQPSTDGDGKTVNFGNDVVDQLVDDDSIGWAQVDQAEALAGMD
                     AGRYYFTVTIPADFSARITSLGEADPKAANIQVTYDDSNSFLASTLGRSAMLEVRDAV
                     AEKVSKEAVSTLLVGVGDARNGFAKASDGAFELSDGLATASDGADRLNVGASDLADGA
                     AKLADGTGDLSTGAHTLADKLGDYVQGISDASAGVEQLRSGAAGLTALQAGLAQAADP
                     TTGAPALAAGVKTLVGGATSLAEGATAYAEGADGFASSAGTWVTGAEQWRQTVAAATG
                     GPLAQGSKDLAAGAKQLSAATASDGSLAQGADAIASGNTKLAAGLGQLDDALAQAQQA
                     LDDGDTATAAAILSSARGKLQDAHANASELAAGAAKVSAGIASVHTGAAGLATGAATL
                     NAGYAQLNGAVADGGAVATGITKLDAGQRALSTAAGQLDAGAAKLADPATLVQAQQLS
                     QGASSLATALQTMSAGASDSAKGIPALQAGLTQLASGFTNADPEHGLVSGAKALRSGA
                     RTLAAGLETAADGATKLSDGSGEIAANTPALASGLADAHSGADELATKLADGAEEIPD
                     DSAAVQESRATAIATPVNTATAHVHEADSWGEGFAPFFISLALWVGALITWLLLRPLQ
                     TRALMTSVNGFRMAWGSLNSALLLSLGQVAIMLAVMHFAIGLTPANLLATFAFTMLAA
                     AAFFSLQQFFQVTLGSAVGKVVIIVLLMVQLASAGGTYPIQTTPSFLQAISPYLPMTY
                     VVNGLREAITGGIETRFWSSVVVLAAIFVVSLAATSLASARKRIWTMSRLHPALSI"
     gene            complement(3234..3986)
                     /locus_tag="PKJ61_04865"
     CDS             complement(3234..3986)
                     /locus_tag="PKJ61_04865"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOC13188.1"
                     /translation="MSSLLTQLPQTETAQPPADPLVAARPLAVAASALQLDGNRGRIY
                     GPVDLAIETGTLNLITGAAGTGKTSLLLTLVGRMRPNRGAELTVLGRPLPRRAPSVQR
                     RSAAVGIHGLDDLDENVTVAATVREREAWLARWYRIVRTPDDARIAQLCAPVFGDDPA
                     PRARQLIHELDEVENLRLRLALAMLSSPEVVVIDDVDSLHDLDSRRSLWESLRALTEQ
                     GITVIATASTAGDLSRLGWADLPNHIALPSVR"
     gene            complement(4240..4455)
                     /locus_tag="PKJ61_04870"
     CDS             complement(4240..4455)
                     /locus_tag="PKJ61_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13189.1"
                     /translation="MKKTMSKRTEAWVYIGAGVVAIFAALYFMSVESSIFDWFILGMG
                     VVSLWQGVRGFMALRNGSSGTPGAGRS"
     gene            complement(4601..5455)
                     /locus_tag="PKJ61_04875"
     CDS             complement(4601..5455)
                     /locus_tag="PKJ61_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HOC13190.1"
                     /translation="MTIAVTGATGHLGGLAITHLLRRGTPAEQVVALVRDVDRAGQLA
                     ALGVQVRHFDFDQPDALAEALADVDALLLVSGNALGRRAAQHRAVIDAALKAGVGKLV
                     YTSAPHVQASVNPVAPEHKATEEALAASGLPHVILRNGWYHENYLRELEAVAETGVLL
                     TAAGDGRVASAARSELAEAAAVVLAGPLDAGTITLTGDVAWTQEDLAADLASVLGRDI
                     EVRHVSALQKADVLAQAGVDAGMAGFAVGVDEAIAAGELGEVNGELARLIGRPTSPLI
                     ETLRAAGR"
     gene            5537..5878
                     /locus_tag="PKJ61_04880"
     CDS             5537..5878
                     /locus_tag="PKJ61_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOC13191.1"
                     /translation="MDRPFDVFSAHCPSRDLVEHVTNRWSSLVLVALLDNPRRFAETA
                     RTVDGISDRMLSRTLTTLVEDGLVTRTEVAGQHVEYDLTPAGRRVAAALRGVVDAVYE
                     VMPTVLSARHG"
     gene            6065..6217
                     /locus_tag="PKJ61_04885"
     CDS             6065..6217
                     /locus_tag="PKJ61_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13192.1"
                     /translation="MAFDRPGRLVAGAIGIDPDLAWDEDGTCYLTWCSFAHDGGIAQA
                     RWDRSH"
     gene            complement(6794..7396)
                     /locus_tag="PKJ61_04890"
     CDS             complement(6794..7396)
                     /locus_tag="PKJ61_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13193.1"
                     /translation="MNEARKPRRPSFREPAALEALGEPSDPFADLHAAHETAAVLLHT
                     GRAADDPAVTARLVTLTEEVGLSTLAEIWSSRPARTLPGALWRLYLLREWVLTKPHEA
                     AREYAAGVRFTEPNHAVAGVDPPGPDEVQRVADEILRGVFAGDFAVALERAAAFCHVV
                     SSGRADVTAGAAAVQQAGRLQTMAEDLTACARLWRAGALE"
     gene            complement(7446..8972)
                     /locus_tag="PKJ61_04895"
     CDS             complement(7446..8972)
                     /locus_tag="PKJ61_04895"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HOC13194.1"
                     /translation="MTRLIEPAATAVTREELPDAVASQLARGLRLALVAGHEDRPTDD
                     FRVVYVLLGAANERVEVVVRVPRDDAWLPTLAGLSYPAGRFERELRDLFGIVPRDHPQ
                     PYRLVRHAHWPTGYHPMLADADPRPVFDGAAGFPFVEVAGKGIYEIPVGPVHAGLIEP
                     GHFRFSVVGETIVRMYQRLWFVHRGVEKLFEGRPVADGIALAERISGDTAVGHSLAYA
                     MAVEDALGIVVPERDRVVRALLLECERLYNHVNDLGAIVNDVGFGIINAHAGRLRERL
                     LRHNARLTGHRLLRGAIRLGGAWLLAEPDLDLLASMAAEVAELAEISTGHSLVADRLD
                     RTAVLPSDQAAQLGVLGYVARASGLGIDARVHQPFVSLGDAFRVVQRETGDVRARFEV
                     RAEEFAVSAAVIADLAARLASSVIPAQAEISTPPRAVRAIQTTATASAGLGVVEAWRG
                     TLVHRVEVNDDGTLARVKVVDPSFCTWPALGVALADTIVPDFPLANKSFNCSYAGNDL
                     "
     gene            complement(8969..10543)
                     /locus_tag="PKJ61_04900"
     CDS             complement(8969..10543)
                     /locus_tag="PKJ61_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOC13195.1"
                     /translation="MAVWIWLLLLLPLFLAGVSYGLRRSAWTRWLPVIAAVLLLVGGI
                     TLVWLVGLSGPVATASGLLRVDALSAWMLVVVGAVAVVALWGGMDRSVSPVPPSSNPV
                     QPSSRRRPGSLSATDGAALRPTTDPILASGSFAALLCLFLAAMTLAVVADNIGLMWVA
                     IELTTITTAFLVASQGGRDSLEAAWKYVVLGAVGVAIAFLGITLLSAASLTSGEATVS
                     WVLLLQHADALDPTLAKAAGALAVLGFATKAGLAPMHSWLPDAHSQAPAPVSGLMSGV
                     LLSVAFYVILRIQAITDAAVGPELMRGLLLVAGLLSLAVTAGLVLTQRDYKRLLAYSS
                     IEHMGLLALGAAAGGPLAIAAVLLHMLGHGLVKSAMFVVAGRILDYVGSHRVADVRNL
                     LRLRPDLAGPFLLGSAALLGFPPFVTFFTEVGILVAGFQSGLLWPMLIACALLLIVFA
                     GIARHVLSMTVGVPADHPQTVPVTRRAPDTSAISPPQRISLPIALALTATTVLGFAWP
                     LLGPLTAAVAALGGAQ"
     gene            complement(10543..11202)
                     /locus_tag="PKJ61_04905"
     CDS             complement(10543..11202)
                     /locus_tag="PKJ61_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13196.1"
                     /translation="MTSERYPQLIALTTGLLLLTAVLQVWGRSLVRSIGLLAAQGVAL
                     AGLVLTVGFHVGEPGALFVAALVLVVKAIVLPWAMLRSARLIGETRERAALVNTAVEL
                     IVAAALTMLAYVVTGSLFGIGSDAAAHAVPVGFALVLLGFWQLLIRRSAVTQLIGFLV
                     LDNGIATVSFLTSGGLPLTVELGASLDVLLVVLILGVLVVRMQAVHGHVDISELKELH
                     D"
     gene            complement(11215..12156)
                     /locus_tag="PKJ61_04910"
     CDS             complement(11215..12156)
                     /locus_tag="PKJ61_04910"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020578949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HOC13197.1"
                     /translation="MTAILVVLVHLAFALAITPVLIGVMRTVRARLEGRSGGGIGQPW
                     RDVLKLLAKEPLRAPGSTPVLSAAPVLLMASSLVLSALVPLVSTLALPSVPGDLFVVV
                     SVLLLGTVALALLGLESGTAFGGMGSSRHMVIAALVEPTVLLSIYAISGPVGSSVLSV
                     IVAARSADPASLLNPVSLLAIVALGVVVIAETGRLPVDNPSTHLELTMVHEAMILESG
                     GRDLGWLEFGSWLRLTALLGLVANLAVPFGVAPDASLVGLLVGVATGAVKLLLAGALL
                     AAVEVFLAKLRLFRVPELLAGSFVLAFLSVSAAYLVF"
     gene            complement(12153..14165)
                     /locus_tag="PKJ61_04915"
     CDS             complement(12153..14165)
                     /locus_tag="PKJ61_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOC13198.1"
                     /translation="MNLVDAGLFAQLCLAAAAILLAVFAPVGHRNLAAGIGVAALGVV
                     GAATGVTALLGVQGAGLRVPLALPGFDAIDPLTLAPDRLGGFFLAIVGVVVAVAAVFG
                     IGYAHGPVASRTGWTAFAAFALGMQLVPSAGDALTFLLAWELMAGGSTVLLLADHAAR
                     EPVRRATLWYAVMTHLSFLLLVAGFGVLIAASGGTTWSRIAATSINEPLASGAFLLLI
                     AGFATKAGLVPVHIWLPRAHPEAPSHVSAAMSAAMVKMGVYGVLLVVLRLLPMGPRWW
                     AIVLVVVGAASALYGILQAAVQSDLKRLLAYSTTENIGLIVTAIGVGLLLRDSGQTGA
                     GDVAMLAALLLAVSHAAFKTVLFLGAGAVLHATGERDLDLLGGLAARMPWTASAFGVG
                     ALSAAALPVTSGFVAEWALLQALVHANSQVDRLIAVVLPITMGVVALTIGLGLLTFVK
                     AFGIGFLARPRTPAAADAHEVGPTMLAALVFGAVVVVGLGLVPGPLAEALATAVNAGG
                     VGTVGATGLALGGFGVLLHPLALTLLTGAVLVPLVAVNFALARRAPRRDVDLAWGCGG
                     ERTSPRMEYNATSYSEPLVRVFRGSLRASRSVDVVRADGAPLLVEEMAFTQETVDVIE
                     DRLYLPAARGVRRFGDFARKLQNGSIHRYIGFSFAALVLVLLWVAR"
     gene            complement(14162..14680)
                     /locus_tag="PKJ61_04920"
     CDS             complement(14162..14680)
                     /locus_tag="PKJ61_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HOC13199.1"
                     /translation="MSTTRLLKLIARTGRIAEPLLAQAETDASPEIQVREGFKPPRAL
                     RGSAQVRHVDAGSCNGCEIEIAAAFGPVYDAERVGARLVASPRHADVVAVTGVVTQNM
                     AEPLVNTVGAVPNPRVVVSIGDCTRGCGLFAGSYAVAGSVDDFVEVDVHVPGCPPPPA
                     AIVAALRAVTGR"
     gene            complement(14677..15030)
                     /locus_tag="PKJ61_04925"
     CDS             complement(14677..15030)
                     /locus_tag="PKJ61_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOC13200.1"
                     /translation="MPLPRHEAKAELFRTLSHPIRIRVLELLAEGERPVYELLANMHT
                     EPSALSQQLAVLRRAGLVRQRRDGGEVLYSLLIPAIGDVLATARVALGELLADQAQLR
                     ADLDAEASASGAARA"
     gene            complement(15510..16934)
                     /locus_tag="PKJ61_04930"
     CDS             complement(15510..16934)
                     /locus_tag="PKJ61_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HOC13201.1"
                     /translation="MQTSSALPIVIQGGMGIAVSSWQLAREVSLAGQLGVVSGTALDG
                     VLARVLQDGDPGGHRRRAMAHFPSPAMAQRILDAYFIEGGRPDGAPYRPHPTLTIAPG
                     KAAIELSLVGNFTEVWLAKEGHSGLVGINMLEKVQTANASAILGAMLADVDYVIMGAG
                     VPREIPRLLTEYAAGRTGHLTIDVVGANRVHRAALDPVMYLGAELPKLRRPKFLAIVS
                     LHVLATYLNKDPEIRPDGFVVEGPRAGGHSAPPRGKLTLDEAGEPIYGPKDDADLEKV
                     AALGVPFWLAGAYASPQMVAEARAAGAAGVQIGTLFAMASESGLTDDLRGRLVAQLAD
                     ETLVVRNDPRASPTGFPFKVAELSDTLSEDANYAARERICDLGYLRSPVERADGKIAY
                     RCASEPVHMYARKGGADEDTSGRKCLCNALMANIGLGQVRRGGYVEEPAVTLGQDLSG
                     AQALLALHPDGWTARQAVDWLLST"
     gene            complement(16994..17980)
                     /locus_tag="PKJ61_04935"
     CDS             complement(16994..17980)
                     /locus_tag="PKJ61_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017936417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC/Alx family metal homeostasis membrane
                     protein"
                     /protein_id="HOC13202.1"
                     /translation="MTLPVLEIAIGSPEIWWITIAAVLALFVLDFVITRRPHEVSMKE
                     AVGWSAFYIALPLAFGVWIWTTFGSERGLEFYAGYLVEKSLSVDNLFVFMLLLSSFAV
                     PRELQQRVLLIGVAGALVLRGVFIALGAAMLANFAWTFLLFGAILLATAVKVLRDTMN
                     PAEEEVNPGELRIVRLVRRFWPVTDGYRGPRMIVVEDGKRALTPLALATLAILATDVV
                     FAVDSVPAVFGITGDAYLVFVTNAFALLGLRALYFVLEGALGALVHLGYGLAAILAFI
                     GAKLVMHWAHGIWSWVPEVDTLFSLGVIVGILAIVITTSLIANKRQARAIQH"
     gene            complement(18114..18467)
                     /locus_tag="PKJ61_04940"
     CDS             complement(18114..18467)
                     /locus_tag="PKJ61_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13203.1"
                     /translation="MPVKTDDFWLLSRAWSTPAGLVRGAQVVFRAIGSAARRRKAVGM
                     GAALASSFLAVALKQGTQVWLSAPVEELIVAGDGSFLRHNLNFTRQRMSWGRFLSAPV
                     SGYAIPLHLAVCRHY"
     gene            complement(18542..18913)
                     /locus_tag="PKJ61_04945"
                     /pseudo
     CDS             complement(18542..18913)
                     /locus_tag="PKJ61_04945"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
     gene            19000..19560
                     /locus_tag="PKJ61_04950"
     CDS             19000..19560
                     /locus_tag="PKJ61_04950"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOC13204.1"
                     /translation="MPRTVRASNRGPAAAAANREAILTSARELLAEQGFQVPLSAIAR
                     RAGVGQGVLYRHFPTRLDLAYAVFEEDFQELENLAAGADGDPFGRLWQRLLALTIEST
                     AFIEMVVVARHEIPDDLGNQRLEAILADPLASAQAEGRVDASWTIADLVLILTMVYGA
                     ASSALDQAEATRKASRALELLGLPGF"
     gene            complement(19702..22410)
                     /locus_tag="PKJ61_04955"
     CDS             complement(19702..22410)
                     /locus_tag="PKJ61_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13205.1"
                     /translation="MSAARRAEVRTSRLRQWRNGWAMALRMARRDVRRDWGRSLFVWL
                     MIVLPIGVIAALLVVVASNTISAAERLDLKLGTAHSVLTQVGYGFQASWDGYGTASSG
                     GKDVEPRPIPGWGNTVAEHQDAVAALIGRPAVAVTETYLEFGKGQVTTTLLGIDPESG
                     ESMFKLISGRNPQTSSEALVTPNGMAAGLPASGTVTLVRDGVARTVTIVGIADAHLDG
                     LVELVGLPEADADRISFFVAAGTPLAWTEAQNLAGYGFETASRGIAADPPSGQEFHPS
                     ELGGPFLTWMVILAAMIEVALVVGPAFAIGAARQRRGLALAASNGASIAQLRRSALGQ
                     ALLLGVSGATLGAALGAGVGVLVWQRMASEPTELHGPLEVPWTQLAGVLALGTLTAVA
                     AALVTARGLARLDLVGSLRGSLRSAPVRKGAPVLGGVLVGAGLVGVWLAMLVAPEWRF
                     LLWTVSALAVLIGVLLCVPALLGVAARLAGGAPMGVRMALRDTSRQRGRATSTVAAIL
                     AGGLILGTVWTLVLSFEADAARRYQPSMPLGQASLYFGGESAAGLRQVIADIDPVLRT
                     YQVGQADGRGSDPEDPQSFAALRPGCTTDDLPAEAGACASLSSDGGWYGQGILIAKVD
                     ELTELFGLDASQRKELAAGKLLVATDTHPNLGDGEANWAEHQIVNGAITFAYATIPES
                     GPTQFELVEVPASPVAWAVIERGASVQWNGALISSESAANLGWVSRSNELRIVDPRGA
                     ISPELEARLKQAITPWGNALSLSVERGYQSQPQPVLWGVTATLVLLAVVAAVIATVMS
                     TIELRPFLETFAAIGAGPGLSRRLAATQAVLLGVLGTTLGCGIGTVVAVPLATSVTGR
                     TDGFMPPVVDFPWLLMAGLVLGIPALAAIVAALCVPARPMLVRRAT"
     gene            complement(22407..23117)
                     /locus_tag="PKJ61_04960"
     CDS             complement(22407..23117)
                     /locus_tag="PKJ61_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002512929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOC13206.1"
                     /translation="MSAPILRLTEVTRVHGSGETAVHALREVSLDVHPGELVAVMGPS
                     GSGKSTLLNLAGALDRPTSGEVEISGQRLSALKPDELARLRRRRVGVVFQDYNLIPSL
                     TAAENIGLPLELDGQHVVAARQEARAVLKELGLGRLADRFPDQLSGGQQQRVAIARAL
                     IGERRLVLADEPTGALDSVTGEDVLSVLRARCDAGAAGLLVTHEPRHAAWADRVVYLR
                     DGVVVDGSAVREPARAIR"
     gene            complement(23114..23752)
                     /locus_tag="PKJ61_04965"
     CDS             complement(23114..23752)
                     /locus_tag="PKJ61_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOC13207.1"
                     /translation="MSVKYGLLALLAEGASYGAALKSAFEARTGGTWPVNVGQVYTTL
                     DRLERDGLVVAGEPDVEGRIAYRLTNAGLEAVERWWRSPVDREVTPREELVIKLAIAI
                     TSPGVDSAEVAQTQRAATMHRLQQLTRLKQSVDPATDLAWLLVVEHQLFAAETEIRWL
                     DQVEASVARYGRPAPFVEVEEQADVRHRYPGAGRDLITQPADSSRDDNEARA"
     gene            complement(23961..24185)
                     /locus_tag="PKJ61_04970"
     CDS             complement(23961..24185)
                     /locus_tag="PKJ61_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13208.1"
                     /translation="MQRQGPNPAPGAPVQGGVLTLGSGDFDETSGKILAAGGTVAVPK
                     AALPGMAWQGYFLDTEGNVFGVHQPDPEAR"
     gene            complement(24707..26353)
                     /locus_tag="PKJ61_04975"
     CDS             complement(24707..26353)
                     /locus_tag="PKJ61_04975"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOC13209.1"
                     /translation="MPDLVVRGDEDLDDAGLVAQTRAGNREAFGVLWRRHAKMAGGVA
                     RSFTNSVDPDDLVAESFARVYLAISNGGGPNDAFRPYLLTTLRNTARTWGRRSHESAT
                     DSIEEYIEEVDPDPILMGLANRELIQRTFASLPTRWQEALWWSEVEGRSASEIAELTG
                     LSDNAAAALCYRAREGLRQAWIQTQVPAESANPECRWTLTRIGAFVRSGLSANHAERV
                     QGHLDDCRRCAGIAARAARIGSTLGIALVGAGSSGIALAAALSATAGAGAVPVTGFAK
                     LKNLTTSNPTVGIAAALVLVLLLAVPATAASGVWRGSSSEAAAAAAAVVPPAVEPPAK
                     VSVPPPPDKTDPAPPVSSEPHPDPTPAVPTASPTPTAGSSSASERKSDGEKPKPEPKV
                     SPTPSATPPAVVPTSTAPTAAVPPTPPASPSVPTEPVVTPSPEIELEPSPTPSEPSPP
                     SESPSPSPTEPTVPPTEPTPSPTEPSPSPTEPGGTTEPDPELDPGGGGGGQCERPGHH
                     QWGCGGWGDHDRDQPDEPKPPQVPDPPLVKEPDPDVFPAP"
     gene            26719..27390
                     /locus_tag="PKJ61_04980"
     CDS             26719..27390
                     /locus_tag="PKJ61_04980"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HOC13210.1"
                     /translation="MTERRTKTADRVPHLPPVRLGQLTEGTADQFAGDEDLDGFSFAG
                     LRFEELVWTGRRRLGSSRVDGATARSWRAPGALFSGSVLTGLDVLTLTADGSGWRQVE
                     LLESRVGSLELFGSTLRSVHFIGCRIGYANLRDTHLTDVAFTDCVIDDLDLMRATATR
                     VALTGTRLRRLELTGAKLADFDLRGAQLAEVSGIEGLRGAIISTEQLVDLAPILAARL
                     GIIVP"
     gene            complement(27396..28331)
                     /locus_tag="PKJ61_04985"
     CDS             complement(27396..28331)
                     /locus_tag="PKJ61_04985"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOC13211.1"
                     /translation="MKTFALGSTELQVPAVVAGVMRIQDKSDAEVRAWYEAALASGIS
                     YFDHADIYGDGDAHGCETRFAEALQLSSAERESIVLQSKAGIVKNGPYFDFSYAHIID
                     AVEGSLAALRTDYLDVLLLHRPDALVEPEEVARAFDELADAGKVRHFGVSNHTPNQIE
                     LLRRHVRQPLVANQLQLSITHAPIVAQGVAMNMADLDQSISRDLGVLDYCRLHDLTVQ
                     AWSPFQAGFFNGVFLGNRGFPELNTVIDRLAAKYEVPTEAIAVAWITRHPARIQVVLG
                     TTTPERVAAAALGADVELTRPEWYELYRAAGHLVP"
     gene            complement(28427..29146)
                     /locus_tag="PKJ61_04990"
     CDS             complement(28427..29146)
                     /locus_tag="PKJ61_04990"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOC13212.1"
                     /translation="MQPSALSRMFDRGASRYDLLVGLNPGYHAELRRAAGALAGRIGP
                     SGAPKVIDLACGSGASTRALLAALPGQPSVLGLDASAGMLAQAIRKSWPDGVRFAEAV
                     AGALDVADLEPGSWDGVFTAYLFRNVPEAGRDRALAEVYELLAPGGWVVVQDYSVAGD
                     RWATLRWHLVSWLVIIPLGLVADRNPGLYRYLWRSVLDFDDRQRFGDRLEAAGFAEVA
                     HRTASGWQRGILHTFVARKPG"
     gene            complement(29218..>31280)
                     /gene="treS"
                     /locus_tag="PKJ61_04995"
     CDS             complement(29218..>31280)
                     /gene="treS"
                     /locus_tag="PKJ61_04995"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009269 - response to desiccation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HOC13213.1"
                     /translation="GRNPAYIAWLEQQSMLHDATVLGRQLAGHHEMWASPYAHPDPRA
                     AVQQASVWFTAYPLSLITRPGQSFLGALGAQELWAAFAEIGIDAIHTGPLKLAGGISG
                     WLPTPSVDGHFDRISMQIDPVFGTEDEFREMCANAARRRGTIIDDIVPAHTGKGADFR
                     LAELNYLDYPGIYHMVNIPEEAWDLLPDVPEGLDSVNLTADAERALAEAGYIIGELQR
                     VIFYEPGVKETNWSVTPPIYDWKGVQRRWVYLHYFKEGQPSINWLDPTFAGMRLVMGD
                     ALHSLLDLGSGGLRLDANGFLGVEKSLDQAPGWSEGHPLSQAANQLIGSMVRKVGGFT
                     FQELNLAMDDILHTSAVGPDLSYDFVTRPAYQFALCTGDTEFLRLTLREALQLGIDQA
                     SLVHALQNHDELTYELVHFAARHRDDAYTLGGVDYTGAELAEHVREAMRAKLTGDSAP
                     YNATFVQNGIACTTASVIAASLGITDLETITDADARRIANAHLLLSMYNAWQPGVFAL
                     SGWDLVGALPLDRAKVRRLISGGDTRWIERGAYDLLGEQPDAKRSSSGMPIATALYGP
                     LPDQLASPTSFASRLAGILKIRKRHGLATGTLLDVPEVAVKSMLVLVNRLESGAIQIT
                     ALNFGAEKISGRVQSEHIPVGRVIDLATRRKVASVDDLGGFTVDLPAFGGLAMVVKEP
                     TPEA"
BASE COUNT         5261 a        10923 c        10662 g         4434 t
ORIGIN      
        1 gggcaactca gcgcagcgtc cccagcaccg cggacgcgac ctgctcggca ctgcgctcgg
       61 ggtggaacac cagccactcc agcccggcca tcagggtggc tccgacgatc gcggccgcct
      121 gcaccccggc cacgaccgga tcgacaccgg cttcacggga gtcctcgacc agcggtgaca
      181 gcgcgatctc acgccaggtc tcgatcgagt cccgccacga gcgctccgtc cggaacatct
      241 cggtcaccat cacccgcgcc gacgacgcgt tctcctgcac cagcagcaac agggcccgca
      301 ccaccgcttc gcgcaacgcg tccccggagc gttcctgctt cgcctcggcc aacgcgccgg
      361 ccagccgctc gctgctgcgg atcatgatcg cctcgaccag gcccgacttg gagtcgaagt
      421 tgtagtagat cgagcccttc gcgatacccg ccgccgcggc gatgtcgtcg atcgaagtcg
      481 aggtcacccc ccgggcggcg aacagttcgg tggcggcgtc catgatcgcg tcccgggtgc
      541 cctggcgccg ggccacccgg ccgtcggtcc ggccgctgac gggtgtggtc atgctcgtct
      601 cctcagatgc tcagcgccgg gtgcagccgc gacatcgtcc aaatccgctt ccgtgcggac
      661 gcgagcgacg tggccgccag cgacaccacg aagatagcgg cgagcacgac gacgctgctc
      721 cagaacctgg tctcgatgcc gccggtgatc gcctcgcgca gcccgttgac cacataggtc
      781 atcggcaggt acgggctgat cgcctgcagg aacgacggcg tggtctggat cggataggtg
      841 ccgcccgccg aagccagctg caccatcagc agcacgatga tcaccacctt gccgaccgcc
      901 gagcccaggg tcacctggaa gaactgctga agcgagaaga acgccgcggc ggcgagcatg
      961 gtgaaggcga acgtggccag caggttggcc ggcgtcagcc cgatcgcgaa atgcatcacc
     1021 gccagcatga tcgccacttg tccgagcgac agcagcagcg ccgagttcag cgacccccaa
     1081 gccatccgga agccgttcac cgaagtcatc aatgcgcggg tctgcagcgg acgcagcagc
     1141 agccaggtga tcagcgcgcc cacccacagc gccagcgaga tgaagaacgg tgcgaagcct
     1201 tcgccccagg aatcggcctc gtggacgtgc gcggtcgcgg tgttgaccgg ggtggcgatc
     1261 gcggtggccc ggctctcctg caccgcggcg gagtcgtcag ggatttcctc ggcaccgtcg
     1321 gcgagcttgg tggccagctc gtccgcaccg gagtgtgcgt ccgccagccc ggacgccagc
     1381 gcgggcgtgt tcgcggcgat ctcgccgctg ccgtcgctca acttggtggc gccatcggct
     1441 gccgtttcca ggccggcggc gagcgtcctc gccccgctgc gcagcgcctt ggccccactg
     1501 accaggccat gttccggatc ggcgttggtg aatccggacg cgagctgggt caacccggcc
     1561 tgcaaggcgg gaatgccctt ggccgagtcg gacgcgcccg cgctcatcgt ctgcaacgcg
     1621 gtggccaggc tggacgcgcc ctggctgagt tgctgtgcct gcacgagcgt cgccgggtca
     1681 gcgagcttgg cggcaccggc atcgagttgc ccggcagcgg tgctcagcgc acgctggccg
     1741 gcgtcaagct tggtgatccc ggtggcgacc gcgccgccgt ccgcgaccgc gccgttgagc
     1801 tgggcgtagc cggcattcaa ggtagccgct ccggtggcca ggccggccgc gccggtgtgg
     1861 acgctggcaa taccggcgct caccttggct gcccccgccg ccagctcgga ggcgttggcg
     1921 tgcgcatcct gcagcttgcc gcgagcgctg gagaggatgg cggcggccgt cgcggtgtcg
     1981 ccgtcgtcga gggcctgctg tgcttgggcg agcgcgtcgt cgagctggcc caggccggcg
     2041 gccagcttgg tgttgccgct cgcgattgcg tccgcgccct gggccaacga gccatcggac
     2101 gcggtggccg ccgagagctg cttggccccg gcggcgagat ccttgctgcc ctgggccagc
     2161 ggtccgccgg tcgcggcggc gacggtctgc cgccactgct ccgcgccggt gacccaggtg
     2221 ccggcgctgg aggcgaagcc gtccgcgccc tcggcatagg cggtcgctcc ctcggccagc
     2281 gaagtagcac cgccgaccag cgtcttcacc ccggcggcca gggcgggcgc gccggtggtg
     2341 gggtcggcgg cctgcgccag gcccgcctgc agcgcggtca gaccggccgc acccgaacgc
     2401 aactgctcga caccggcgct cgcgtccgag atgccctgca cgtagtcgcc gagcttgtcg
     2461 gcgagcgtgt gcgcgccggt ggagaggtcg ccggttccgt ccgccagctt cgcggcaccg
     2521 tcggccaggt cggacgcacc gacattcagg cggtcggcac cgtcgctggc cgtcgccagc
     2581 ccgtcggaca gttcgaacgc accatccgag gccttggcga agccgttccg ggcatcgccg
     2641 acgccgacca gcagcgtgct gacggcttcc ttcgagacct tctcggccac cgcgtcgcgc
     2701 acctcgagca tcgccgaacg gccgagggtg gaagccagga aggagttcga gtcgtcgtag
     2761 gtcacctgga tattggcggc tttggggtcg gcttcaccga gcgaggtgat ccgagcggag
     2821 aaatccgccg ggatggtgac cgtgaagtag tagcggcccg cgtccatgcc ggcgagcgct
     2881 tcggcctggt cgacctgagc ccagccgatg gagtcgtcgt cgactagctg gtcgaccacg
     2941 tcgttgccga aattgacggt cttgccgtcg ccgtcggtgc tgggctgatc ctggttgacc
     3001 aatgccaccg gcaggtcgtt catgcgggtg gtcgggtccc agaacgccca gacgtacatc
     3061 gcgccgtaga gcagcgggat cagtagcatc accgccacgg cggcgcgggt gatgggcagg
     3121 cgggcgaacc gtttcagttc ggttccgtgt gaggtccagg caaacatcta ttcttctgcg
     3181 attcgggttg atttggcccg ccttgttcgc gggcaagttg gggctggctg ggttcagcgg
     3241 acgctgggca gcgcgatgtg gttgggcagg tcggcccagc cgagccggga gaggtcgccg
     3301 gcagtggacg cggtcgcgat cacggtgatt ccttgctcgg tcagcgcacg cagcgattcc
     3361 cagagacttc ggcggctgtc caggtcgtgg agggagtcga catcgtcgat cacgaccacc
     3421 tcgggtgagc tcagcatggc caatgccaac cgcagccgca ggttctcgac ctcatccagt
     3481 tcgtggatca gctggcgggc gcgcggcgcc ggatcgtcgc cgaagaccgg cgcgcagagc
     3541 tgcgcgatgc gcgcgtcgtc gggtgtgcgg acgatccggt accagcgggc cagccaggcc
     3601 tcgcgctccc ggacggtggc ggcgacggtg acgttctcgt ccagatcatc gaggccatgg
     3661 atgccgaccg ccgcgctgcg ccgctgcacc gacggtgccc gacgcggcaa cggccggccg
     3721 agaacggtga gctcggcgcc gcgattgggc cgcatccgtc cgaccagggt gagcagcagc
     3781 gaggtcttgc cagtgccggc cgcaccggtg atcaggttga gcgtgccggt ttcgatggcc
     3841 aggtcgaccg ggccgtagat gcggccgcgg ttgccgtcca gctggagggc ggacgcggcc
     3901 accgcgagcg ggcgagcagc aaccagcgga tcggccggcg gctgcgcggt ctcggtttgg
     3961 ggaagctggg tcagcaggct gctcatgaac ggttcctttc gtcacatcga gagtagttga
     4021 actgaccggt cattacaaat acatgccgtt cgctccaact catggacacc catcgcactc
     4081 cctggagtcc gcacaggatc gtgcgggctg ggaccggcca ccaacgcgca aactggacac
     4141 ttccagccgt tgtcggctgg aagtgtccag tttgagttgg ggcggggcca gaccacaggg
     4201 tcgtcgaccc cggccgggtc gcttagcttg gcagaaggct caactccggc cggcgcccgg
     4261 cgtcccgctg gagccattgc gcaacgccat gaacccgcgg acgccctgcc agagcgagac
     4321 cacccccatg ccgaggatga accagtcgaa gatgctggac tcgacgctca tgaagtaaag
     4381 ggcggcgaag atagccacca ctccggcacc gatgtacacc caggcctcgg tccgcttgga
     4441 catggtcttc ttcatgcatc gcagcctaga cgagtcgcgt aaccccgcac gcaccgggca
     4501 catcctctcg tgcgcactgg gctacgcaaa cacgccctag acgagggtgc gagccagggc
     4561 gggacgcggg ccccatagcg tcccgccccg gccggcacgt tcagcgtccg gcggcccgca
     4621 gggtctcgat caggggtgag gtcgggcgac cgatcaggcg ggccagctca ccgttcacct
     4681 cgcccagctc gccggcggcg atcgcctcgt ccacgccgac cgcgaagccg gccatgcccg
     4741 cgtccacgcc cgcttgcgcc agcacgtccg ccttctgcag cgccgaaacg tgccgcacct
     4801 cgatgtcgcg tcccagcacg gacgcgaggt cggcggccag atcctcctgc gtccaggcga
     4861 catcgccggt gagcgtgatc gtgcccgcgt ccagcggtcc ggcgagcacc accgcagcgg
     4921 cttcggcgag ttcgctgcgg gcagcactgg ccacccgtcc gtcgccggcg gcggtaagca
     4981 gcaccccggt ctcggcgacc gcctcgagtt cgcgcaggta gttctcgtgg taccagccgt
     5041 tgcgcaggat cacgtgcggc agaccggacg cagccagcgc ctcctcggtg gccttgtgct
     5101 cgggcgcgac cggattgacg gacgcctgca cgtgcggcgc actggtgtag acgagcttgc
     5161 caacgccggc cttgagcgcg gcatcgatga ccgcccggtg ctgggcggcc cgccggccga
     5221 gcgcattgcc ggagacgagc agcagcgcgt ccacgtcggc aagggcttcg gcgagcgcgt
     5281 ccggctggtc gaagtcgaag tgacgcacct gcacgccgag cgcggcaagc tggccggcac
     5341 gatcgacgtc gcggacgagc gccaccacct gttcggcggg cgtgccgcgc cgcagcagat
     5401 gcgtgatcgc gagtccgccg agatggccgg tggcgccggt gacggcaatg gtcatgtttt
     5461 cctccgaagt tgcttacgat tggttagcag tatgcccgta ctatacttcg gttagcaact
     5521 cacgcaaggg gaagtcatgg atcggccatt cgacgtcttc agcgcgcact gcccgtcccg
     5581 cgacctcgtg gagcacgtga ccaaccggtg gtcttcgctg gtgctggttg ccctgctcga
     5641 caatccccgc cggttcgccg agaccgcccg cacggttgac gggatcagcg accgcatgct
     5701 ctcgcggacg ctcactaccc tcgtcgaaga cggcttggtc actcgcaccg aggtcgccgg
     5761 ccagcacgtc gagtacgacc tgaccccagc cggacgccgg gtcgccgcgg ctctgcgcgg
     5821 cgtggtcgac gccgtctatg aggtcatgcc gaccgtgctg agcgctcgcc acggctagcg
     5881 tgaatccacg ggcctgagcg gggttcaaca agctcgacca gccgaattag tggatccggg
     5941 cttgcctgtt gccgtaggca tcgattcgcc aacaaccgga agcaattcac cggacgatct
     6001 gccgggtggg cgcggagtac ttcctcgcca actcgagctt cgagtacttc cctggagccc
     6061 cgctgtggcg ttcgatcgac ctggtcgact ggtcgccggc gccatcggca tcgatcccga
     6121 cctcgcctgg gacgaggacg gcacctgcta tctgacctgg tgcagcttcg cgcacgacgg
     6181 cgggatcgcc caggccaggt gggaccgttc gcactgacct tcagccaagc aacggcgccg
     6241 tcgagcgagg tcaccctggt gattgcggcc gggccggcac caggggccga cgcactggga
     6301 agcaacctag gaccggacga ggttcaactc ggctatcgct ccggcggggc gctggtggcg
     6361 ctcacccggc tggatggccg ctacttcagc cccgaggtgg ccggcggttt caccggaagg
     6421 gcgccgggac gctggtgcag gccgcgagga gcgaggttag gagaccgatc gcgccgaaca
     6481 gcgcaagcct ggggtgggca acatccagtc agcctaaccg gagtggtggt gagagcgtcg
     6541 ggccgggagc gcctctcggc gcgtggccgc tcgtagcggc gaatgttgga gcccggggct
     6601 accgcggccc gacgcctgat cgaggatagg cgtcccccgc cgctccccca aatcttgaac
     6661 ggatagggga cggttccttt ctcgttcaag ttatccggga cgcgtcttgc ggtcgcgggc
     6721 tcgacacggg cgggcccagc tcggggacgg tctgtggaca agctgaacgg aaaaggaacc
     6781 gtccccttct ccttcactcc agggcgccag cgcgccagag tcgggcgcag gcggtgaggt
     6841 cctcggccat ggtttgcagg cgtccggcct gctggacggc agccgcgcct gcggtgacgt
     6901 ccgcgcgtcc ggacgagacc acgtggcaga acgccgcggc gcgctccagc gccaccgcga
     6961 agtcgccggc gaacacgccg cgcaggatct cgtccgccac tcgctgcacc tcgtccggac
     7021 cgggcgggtc gacgccggcc accgcgtggt tcggttcggt gaagcggacg ccggccgcgt
     7081 actcgcgcgc agcctcatgc ggcttcgtga gcacccactc ccgcagcagg taaagccgcc
     7141 agagcgcacc gggcagcgtc cgcgccggac gcgaggacca gatctctgcc agcgtcgaca
     7201 ggcccacctc ctcagtgagc gtcaccaatc gggccgtgac cgcggggtcg tcggcggcgc
     7261 gtccggtgtg cagcagcacc gccgcggtct cgtgcgcagc gtgcaggtca gcgaacgggt
     7321 cggacggctc ccccagcgcc tccagggccg cgggctcccg gaagctcggc cggcggggtt
     7381 tgcgggcttc attcacccgg caagggtagt tcgaatccga gggtcggggg tcggggcccg
     7441 gactgttaga ggtcgttgcc ggcgtatgag cagttgaagc tcttgttggc cagcgggaag
     7501 tcggggacga tcgtgtccgc caacgccacg cccagcgcgg gccaggtgca gaaggacggg
     7561 tcgacaacct tcactctggc gagcgtgccg tcgtcgttga cctcgacccg gtggacgagg
     7621 gttccgcgcc aggcctcgac gacgcccagg ccggcggacg cggtcgcggt ggtctggata
     7681 gcccggacgg ctcgcggtgg ggtggagatt tcggcctgcg ccgggatgac ggacgaggcc
     7741 agccgagcag cgagatcggc gatcacggca gcggaaactg cgaactcctc ggcgcgcact
     7801 tcgaagcggg cgcggacgtc accggtctcg cgctgcacca cccggaacgc gtcacccagc
     7861 gagacgaacg gctggtgcac ccgggcatcg atgccaagac cggacgcccg cgccacgtag
     7921 cccagcactc cgagctgcgc ggcctggtcg gacggcagaa ccgcggtccg gtccaggcgg
     7981 tcggccacca gactgtgccc ggtcgagatc tcggcaagct cagccacttc agcagccatg
     8041 gacgcgagca ggtccaggtc aggttcggcg agcagccagg caccacccag ccggatcgcg
     8101 ccgcgcagca atcgatgacc ggtgagccgg gcattgtgcc gtagcaggcg ctcgcggagg
     8161 cgtccggcat gggcgttgat gatgccgaac ccgacatcgt tcacgatcgc gccgaggtcg
     8221 ttgacgtggt tgtacagccg ctcgcattcc agcagcagcg cccgcaccac ccggtcgcgc
     8281 tcgggcacga cgatgcccag cgcgtcctcg accgccatcg cgtaggccag gctgtgtcca
     8341 accgcggtgt cgccgctgat ccgctcggcg agcgcgatcc cgtccgcgac tggacgtcct
     8401 tcgaagagct tctccacgcc gcgatggacg aaccacaacc gctggtacat ccggacgatg
     8461 gtctcgccga ccacggagaa gcggaaatgc cccggctcga tcagtccggc gtgcaccggc
     8521 ccgaccggga tctcgtagat tcccttgccg gcgacttcga cgaacgggaa gccggcggca
     8581 ccgtcgaaga ccggacgcgg gtcggcgtcc gccagcatcg ggtggtagcc cgtcggccag
     8641 tgcgcgtgcc gcaccaggcg atagggctgc ggatggtccc gcggcacaat gccgaacagg
     8701 tcccgcaact cacgctcgaa gcgtccggcc gggtaggaca ggccggccaa ggtgggcagc
     8761 caggcgtcgt cgcgcggcac ccgcacgacc acctccaccc gctcattcgc ggcgccgagc
     8821 agcacgtaca ccacgcggaa gtcatcggtc ggacggtcct cgtggccggc caccagcgcc
     8881 agacgcagcc ctcgcgcgag ctgggacgcg accgcgtccg gcaactcctc gcgggtgaca
     8941 gctgtggccg cgggttcgat caggcgcgtc attgggcacc tcccagggca gcgacggctg
     9001 cggtgagcgg gccgagtagt ggccaggcaa acccgagcac ggtggtcgcg gtgagggcta
     9061 gggcgatcgg gaggctgatt cgttgaggtg gtgaaattgc agaggtgtcg ggggcccgtc
     9121 gtgtcaccgg gaccgtttga gggtggtctg ccggcactcc cacagtcatc gaaaggacgt
     9181 ggcgggcgat gccggcgaag acgatcagca ggagcgcgca ggcgatcagc atcggccaca
     9241 acaggccgct ctggaaaccg gccacgagga tgccgacctc ggtgaagaag gtcacgaacg
     9301 gcgggaagcc gagcagagcg gcggagccga gcaggaacgg tcccgcgaga tcaggacgca
     9361 ggcgcagcag gttccgtacg tccgccaccc gatggctgcc gacgtagtcg aggatgcgtc
     9421 cggcaaccac gaacatggcc gacttcacca agccgtggcc aagcatgtgc aacagcaccg
     9481 cggcgatcgc cagcggacca ccggcggcgg cgcccagagc cagcaggccc atgtgctcaa
     9541 tactgctgta ggcgagcagg cgcttgtagt cgcgctgggt gagcaccagt ccggcggtca
     9601 cggccaacga cagcaacccc gcgaccagca ggagaccgcg catcagttcc gggccgacgg
     9661 ccgcgtccgt gatcgcctgg atccgcagga tcacatagaa cgccaccgac aacagcaccc
     9721 ctgacatcag cccgctgacc ggggcgggcg cctggctgtg cgcgtccggc agccaggagt
     9781 gcatgggcgc cagcccggcc ttggtggcga agcccagcac cgccagcgcg ccggctgcct
     9841 tcgccagggt cgggtcgagc gcgtccgcgt gttgtagcag cagcacccag gagacggtcg
     9901 cttcgccgga cgtgaggctc gccgccgaca gcagcgtgat gccgaggaat gcgatcgcca
     9961 caccgaccgc gccgagcacg acgtatttcc aggcggcctc cagcgaatcc cgtccgccct
    10021 gggacgcgac caggaaagct gtggtgatcg tggtcaactc gatggccacc cacatcaggc
    10081 caatgttgtc cgccacgacc gcgagggtca tcgcggccag gaacaggcag agcagcgccg
    10141 cgaatgagcc gctggcaagg attgggtcag tcgtcgggcg cagggcggct ccatcggtcg
    10201 cgctgagaga tcccggccta cgccgggatg acggttgaac cgggttcgat gacggcggta
    10261 ccgggctcac cgaacgatcc atcccacccc agagcgcgac cacggctacg gcgccgacga
    10321 ccaccagcat ccaggcgctc agggcgtcca cgcgtagcag tccggacgca gtcgccactg
    10381 gccccgacaa cccaaccagc cacacgagcg tgatcccacc aaccagcagc agcaccgcgg
    10441 cgatgacggg caaccagcgc gtccacgccg accgacgcaa gccgtagctc actccagcta
    10501 ggaacaacgg cagcaggagc agcaaccaga tccagacagc catcagtcgt gcaactcctt
    10561 cagctcggag atgtcgacat ggccgtgcac cgcctgcatc cgcaccacca ggacgccgag
    10621 aatcaatacc accagtagta catcgaggga cgcgccgagc tcgaccgtca gcggcagccc
    10681 gccggaagtc aggaaagaca ccgtggcgat gccgttgtcc agcaccagga atccgatcag
    10741 ctgggtgacc gccgagcggc ggatcagcag ttgccagaag cccagcagca ccagggcgaa
    10801 ccccaccggt actgcgtgcg cagccgcgtc cgaaccgatg ccgaagagcg aaccggtcac
    10861 gacgtaggcg agcatggtca gggcggccgc gacgatcagt tcgaccgcgg tgttcaccag
    10921 cgcggcccgc tcgcgggtct cgccgatcaa ccgggccgac cgcagcatcg cccagggcag
    10981 cacgatcgcc ttcaccacca gcaccaacgc cgcgacgaag agcgcccccg gttcgccaac
    11041 gtggaacccg accgtgagca ccagcccggc cagcgccacg ccctgggcag caagcagtcc
    11101 gatcgaccgc accagcgagc gtccccagac ctgcaggacg gcggtcagga gaagcagtcc
    11161 ggtggtcagg gcgatcagct gcggataccg ctcggacgtc atcagcgcac cccctcagaa
    11221 caccaggtag gcggcgctga ccgaaaggaa cgccagaacg aaggagccgg cgagcagttc
    11281 cggcacccgg aacagccgca gcttggccag gaacacctcg acggccgcca gcagggcgcc
    11341 ggcgagcagc agcttcaccg cgccggtcgc gacccccacc agcaacccga ccaacgatgc
    11401 gtccggcgcc accccgaacg gcaccgcgag gttggccacc agcccgagca gcgcggtcag
    11461 ccgcagccag gagccgaact cgagccagcc gaggtcgcgt ccgccggatt cgaggatcat
    11521 cgcctcgtgc accatcgtca gttccaggtg cgtggacggg ttgtcgacgg gcaggcgtcc
    11581 ggtttcggcg atcacgacca cccccagagc gacgatagcg agcaggctca ccgggttgag
    11641 cagggacgcc gggtcggcgc tgcgggccgc gacgatcacc gaaagcaccg aagacccgac
    11701 cggaccgctg atcgcgtaga tgctcagcag cactgtcggt tccaccagcg cggcgatcac
    11761 catgtgccgg ctcgagccca tgccgccgaa agcggtcccg gattccaggc cgagcagggc
    11821 cagcgccacc gtcccgagca gcagcaccga caccaccacg aacaggtcgc ccggcacgct
    11881 gggcagcgcg agcgtgctga ccagcggcac caatgcgctc agcaccaggc tggacgccat
    11941 cagcagcacc ggggccgcgc tcagcaccgg ggtcgagccc ggagcgcgca gcggctcctt
    12001 ggcgagcagc ttgagcacgt cccgccacgg ctgcccgatg ccgccgccgc tgcggccctc
    12061 cagccgcgcg cgaaccgtcc gcatcacgcc gatcagtacc ggcgtgatgg cgagcgcaaa
    12121 cgccaggtgg acgagtacca ccaagatcgc ggtcatcgcg ccacccacag caggaccagc
    12181 accagcgcgg cgaacgagaa gccgatgtag cggtggatgc tgccgttctg cagcttgcgg
    12241 gcgaagtcgc cgaagcgtcg aacgccgcgc gcggccggca ggtagagccg gtcctcgatc
    12301 acgtcgacgg tctcctgggt gaaggccatc tcctcgacca gcagcggggc gccgtcggcg
    12361 cggacgacgt ccaccgaccg gctggctcgc agcgacccac ggaagacccg gaccagcggc
    12421 tcggagtagc tggtcgcgtt gtactccatt cgcgggctgg tgcgctcgcc gccacaaccc
    12481 caggccaggt cgacgtcgcg gcgcggcgcg cgccgcgcaa gcgcgaagtt caccgcgacc
    12541 agtgggacga gcaccgcccc ggtcaggagc gtgagtgcga gcgggtgcag caggacaccg
    12601 aagccgccaa gcgccaaccc ggtggcaccg actgtgccga cgccgccggc gttgaccgcg
    12661 gtggccagtg cttcagcgag cgggcccggc accaggccga ggccgaccac gacgaccgcc
    12721 ccgaagacca gggccgccag catggttggg ccgacctcgt gggcgtccgc tgctgccggg
    12781 gtgcgcgggc gggcgagaaa gccgatgccg aacgccttga cgaaggtgag caggccaagc
    12841 ccgatcgtca gtgcgaccac gcccatcgtg atcggcagca ccaccgcgat cagccggtcc
    12901 acctgcgagt tcgcgtgcac cagcgcctgc agcaacgccc actcggccac gaaaccggac
    12961 gtgaccggca gcgcggcggc cgacagcgcg cccacgccga aggcggacgc cgtccacggc
    13021 attcgggcgg ccaggccgcc gagcaggtca agatcgcgct cgccggtggc gtgcagtacc
    13081 gcgccggccc cgaggaacag gacggtcttg aaagcggcgt ggctaaccgc gagcagcaac
    13141 gcggccagca tcgcgacgtc gccggcaccg gtctggccgg aatcgcgcag cagcagtccg
    13201 accccgatcg cggtgacgat cagtccgatg ttctcggtgg tcgagtaggc cagcagccgc
    13261 ttcaggtcgg actggacggc ggcctgcaga atcccgtaga gagcggacgc cgcacccacc
    13321 acgaccagca cgatcgccca ccagcgcggg cccatcggca gcagccgcag caccaccagc
    13381 agaacgccgt agacacccat cttgaccatg gccgcgctca tcgcagcgga gacgtggctc
    13441 ggcgcctccg gatgggcgcg aggcagccag atgtgcaccg gcaccagccc ggccttggtg
    13501 gcgaaaccgg cgatgagtag gaggaatgca ccactggcca ggggctcgtt gatacttgtt
    13561 gccgcgattc gggaccatgt cgtgccgccc gaagcggcga tcagtacccc gaaaccggca
    13621 accaacagca ggaagctgag gtgggtcatg accgcgtacc agagggtcgc gcggcggacg
    13681 ggctcgcggg cggcgtggtc ggcgagcagc agcaccgtgg acccgccggc catgagttcc
    13741 caggccagca ggaacgtcag tgcgtcccca gcggacggaa ccaactgcat gccgagggcg
    13801 aaggcggcga aggccgtcca accggtgcgg gaggcgaccg ggccgtgggc gtagccgatg
    13861 ccgaagaccg cggcgaccgc gaccaccacg ccgacgatcg cgaggaagaa gccgccgagc
    13921 cggtcggggg cgagcgtgag cggatcaatc gcgtcgaagc cgggcagcgc gagcgggact
    13981 cgcaagccgg cgccctggac gccgagcagg gccgtcactc cggtcgcggc gccgacgacg
    14041 ccgagggcgg ccacgccgat gccggcggcg aggttgcggt gcccgaccgg tgcgaacact
    14101 gcgagcagga ttgcagccgc ggcgaggcaa agctgggcga acaggcccgc gtccacgagg
    14161 ttcatcggcc ggtcaccgcg cgaagggcgg ccacgatagc cgccggcggc ggcggacagc
    14221 cgggcacgtg cacgtccacc tcgacgaagt cgtcgaccga accggccacg gcgtaggagc
    14281 cggcgaacag tccgcagccg cgggtgcaat cgccgatgga gacgaccacg cgagggttcg
    14341 gcacggcccc gacagtgttg accagcggct cggccatgtt ctgggtgacc acaccggtca
    14401 cggccaccac gtccgcgtgc cggggggacg cgaccagccg cgcgccgacg cgctcggcgt
    14461 cgtagaccgg gccgaatgcg gccgcgatct cgatttcaca gccgttgcac gagccggcgt
    14521 ccacgtggcg cacctgggcg ctgccgcgaa gcgcgcgcgg tggcttgaag ccttcacgta
    14581 cctgaatctc gggactggcg tcggtctcgg cttgggctag tagcggctca gcgatccggc
    14641 cggtgcgggc gatgagcttc agcagccggg tggtactcat gcgcgcgcag caccgctggc
    14701 cgaagcctcg gcgtccaggt cggcgcgcag ctgggcctgg tcagccagca gttcaccgag
    14761 agctacgcgt gcggtagcga gcacgtcgcc gatcgccggg atcagcagtg agtagagcac
    14821 ctcgccgccg tcccgccgct gccggacgag gcccgcgcga cggagcacgg caagctgctg
    14881 cgacaacgca ctgggttcgg tgtgcatgtt cgcgagcagt tcatagaccg gacgctcgcc
    14941 ttcggccagc aactcgagca cgcggatccg gatgggatgg ctgagggtgc ggaagagctc
    15001 ggccttcgct tcgtggcgag gcaggggcat gcagactcct cgaattgcta acttctgcat
    15061 gtacgttaca gcaacgacgc ccactcaggc cgcgttgctc aacaatcctg acgggaatcg
    15121 gaccgccgag agggccgtag ccgcccatag ccactacaga cacgcaccgg gccaaatcac
    15181 gtcacgctcg aagacgaaaa ccgcgccgag cgggaccacc gtgtcgtgat ctcggcaaga
    15241 tcgggcccgg cttggcgctg aacgcaaccc ggacgccacc atggctgcga gatcacgtca
    15301 cggctgcggt ctcgaccggc tcgaccacca ggctggagcc gccaacagac tggagccgcc
    15361 aacccagact tcgctcccga atgggaccct tgctcccgat aattcgggag cgcgagtccc
    15421 attcgggagc gaagtgcagc tgggcaggaa cctctgcgtg ctgcccggca ttgccggccg
    15481 gtagcggacg agccggggcg gacgggcggt caggtggaga gcagccagtc gacggcctgg
    15541 cgagcggtcc agccgtccgg gtgcagagcc agcaaggctt gagcgccgct gaggtcctga
    15601 ccgagggtga ccgcgggctc ctcgacgtag ccgccgcggc gcacctgccc cagcccgatg
    15661 ttggccatca gcgcgttgca caggcacttg cggccgctgg tgtcctcgtc cgcaccgccc
    15721 ttgcgcgcgt acatgtgcac cggctcggac gcgcagcggt aggcgatctt gccgtccgca
    15781 cgctcgacgg gcgagcgcag atagcccagg tcgcagatcc gctcccgggc cgcgtagttg
    15841 gcgtcctcgg aaagggtgtc gctcagctcg gcgaccttga acgggaaccc ggtcggcgat
    15901 gcgcgggggt cgttgcgcac gaccagggtc tcgtccgcga gttgcgccac cagccgcccg
    15961 cgcagatcgt cagtcagtcc ggattcactg gccatcgcga acagcgtccc gatctggacg
    16021 ccggccgcac cggccgcgcg ggcctcggcc accatctggg gggacgcgta cgcgccggcc
    16081 agccagaacg gcacccccag ggcagccacc ttctccagat cggcgtcgtc cttcggcccg
    16141 tagatcggct cccccgcctc gtccagggtc agctttccgc gcggcggcgc gctgtgcccg
    16201 ccagcgcgtg ggccctcgac gacgaacccg tccggacgaa tctccggatc cttgttcagg
    16261 tacgtcgcca gcacgtgcag tgagacgatc gccaggaact tcggccgccg cagcttgggc
    16321 agctcagcac cgaggtacat caccgggtcg agcgcggcgc gatgcacccg attggcgccg
    16381 acgacgtcga tggtcaggtg gccggtgcgc ccggcggcgt actcggtgag cagtcgcgga
    16441 atctcgcgcg gcacgccggc gcccatgatc acgtagtcga cgtcggcgag catcgcaccg
    16501 aggatggcgg acgcgttcgc ggtctgcacc ttctccagca tgttgatgcc gaccagcccg
    16561 gaatggccct ccttcgccag ccacacctcg gtgaagttgc ccaccaggct gagttcgatg
    16621 gctgccttcc cgggagcgat cgtcagcgtg gggtgcggac ggtagggcgc gccgtcgggg
    16681 cgcccaccct cgatgaagta ggcgtccagg attcgctgcg ccatcgccgg cgacgggaag
    16741 tgcgccatcg cgcgacgccg atgaccgccg gggtcgccgt cctgcaggac gcgggccagc
    16801 accccgtcca gagcggtgcc ggaaaccacg ccgagctgtc cggcaaggga tacctcgcgg
    16861 gcaagctgcc aggaagagac cgctatgccc atgccgccct gaataacgat cgggagggct
    16921 gaggaggttt gcattacctt acgataccgt aggttacggc ttcgtaggta gtcggctgct
    16981 gatacgaaac cagctagtgc tgaattgcgc gcgcctggcg cttgttcgcg atcaggctgg
    17041 tggtgatcac gatggccagg atgccgacga tcactcccag cgagaacagc gtgtccacct
    17101 cgggcaccca cgaccagatg ccgtgcgccc agtgcatcac gagcttggcg ccgatgaacg
    17161 cgaggatcgc ggccaggccg tagccgagat gcaccagcgc acccaacgcg ccttcaagca
    17221 caaagtagag cgcacgcagg cccagcaggg cgaaggcgtt ggtgacgaac accagatagg
    17281 cgtcccccgt gatgccgaag accgcaggca ctgaatcgac cgcgaacacc acgtccgtag
    17341 ccaggatcgc cagcgtggcc agcgccaggg gcgtgagggc gcgcttgccg tcctcgacca
    17401 caatcatccg cgggccgcgg tagccgtccg ttaccggcca gaaccgccgc accaagcgga
    17461 cgatgcgcaa ctcgcccgga ttcacttcct cctcggcggg attcatcgtg tcccgcagca
    17521 ccttcaccgc ggtggccagc aggatcgcgc cgaacagcag gaacgtccac gcgaagttcg
    17581 ccagcatggc cgcgccgagg gcgatgaaca cgccgcgcag caccagggca ccggcgacac
    17641 cgatcaacaa caccctctgc tgcagttcac ggggtaccgc gaacgagctg agcagcaaca
    17701 tgaacacgaa caggttgtcg acgctgagcg acttctccac cagatagccg gcgtagaact
    17761 ccaggccgcg ctcggacccg aaggtcgtcc agatccagac gccgaaggcg agcggcagcg
    17821 cgatgtagaa cgccgaccag ccgaccgcct ccttcatcga cacctcgtgc ggacgccggg
    17881 tgatcacgaa gtcgagcacg aacagtgcca gcacggcagc gatggtgatc caccagattt
    17941 cgggcgatcc gatggcgatt tcgagtacgg gcaaggtcat tgggcctcct cacggcaatg
    18001 ccggtgaggt ctccttcacc gtctccggcc cagctgcgag aacgcgattg cgcgttcgta
    18061 atgaccgcca actgactggg aagtactccc ctcaccgaaa tcgtagagca ggtttagtaa
    18121 tgtcgacaaa ccgctaagtg cagtggaatt gcgtagcccg acactggcgc ggaaaggaac
    18181 cgtccccaac tcatccgctg cctagtgaag ttgagattgt gacgcagaaa ggaaccgtcc
    18241 cccgcaacga tgagttcctc gaccggtgcc gagagccaga cctgcgtccc ctgcttcagc
    18301 gcgaccgcaa ggaaggagga cgccagggcc gcgcccatgc cgacggcctt gcgccgccgg
    18361 gccgcgctgc cgatggcgcg gaacaccacc tgcgcgccgc gcaccaggcc ggccggggtc
    18421 gaccaggccc gggacagcag ccagaagtcg tcggtcttga ctggcatcgg gacgccgtcc
    18481 tggcccgcga agtggcccac cactcgccga tctcgcgaac gtcgaaaggc tccacctcga
    18541 tcgcacggcc gatcttgccg ccgggacgtt cggggtagta atccgggtag tccttggcgc
    18601 gggcgaattt catgccgagc cgctcggcga ggttcacgaa cccggcgacc ccgtcgacga
    18661 aggcctcctt gcggacgcgg gacgtcgccg gtccggcatc gccgaccgtc gcttccagat
    18721 aggcgagggc gtcttcgcgg gagtcgccgg cgccggcgcg ctgcatcagc gggttgttcg
    18781 gcagccacag gcccccgccg gacatggcgc tgctgccgcc ccagcgggga gtgctctcaa
    18841 gcatcagcac cttcagcccg gcgtcggcag cggttatcgc agcagcgaaa gccgccccgc
    18901 cggttccgcc caccactacg tcgaatacct gatcccactg ctcgcttatg accggcctcc
    18961 aaccaaccgg acaaccttgt ccggttctag actgccagca tgccaaggac cgtccgcgcg
    19021 agcaataggg gcccggctgc agctgcggcc aatcgggagg cgatcctcac ctccgcccgg
    19081 gaactgctgg ccgagcaggg tttccaggtg ccgttgagtg cgatcgcgcg ccgcgccggg
    19141 gtcggtcaag gcgtgctcta ccggcatttc cccacccgcc tcgacctggc ctacgccgtg
    19201 ttcgaggaag acttccagga actcgaaaat ctcgctgcag gcgcggacgg ggatccgttc
    19261 ggccggctct ggcagcggct gctcgccctg accatcgagt cgaccgcctt catcgagatg
    19321 gtggtcgtgg cgcggcacga gatccccgac gacctgggca accagcggct ggaggcgatc
    19381 ctggcagacc ccctcgcgtc cgctcaggcc gagggccgcg tggacgcaag ctggacgatc
    19441 gccgacctgg tgctgatcct gaccatggtc tacggcgccg ccagctcagc gctcgaccag
    19501 gccgaggcca ctcgcaaagc cagtcgcgcc ctggagctcc tgggcctgcc cggcttctga
    19561 atctccgggg acggttcctt tgcacggcgt cagactgtgg ctgcgcgcac cgccgacggt
    19621 ggactgagcc acagtcaagc ggtccgtgcc gtagtccctc gccaatacgg acggagcgtg
    19681 caccaaatga tggcgcaggc gtcaggtcgc gcgacgcacc agcatcggac gggctgggac
    19741 gcacagcgcg gccacgatcg cggccaaagc cgggatgcct agtaccaggc cggccatcag
    19801 cagccagggg aagtcgacca ccgggggcat gaacccgtcc gtacgtccgg tcaccgaggt
    19861 ggccagcggc accgcgacca ccgtaccgat cccgcagccc agcgtcgtcc cgagcacacc
    19921 gagcagcact gcctgcgtcg cggccagccg ccggctcaac ccaggcccgg cgccgatcgc
    19981 tgcgaacgtc tcgaggaagg gccgcagttc gatcgtagac atcaccgtcg cgatcaccgc
    20041 ggccaccacg gccagcagca ccaaggtcgc ggtcacaccc cacagcacgg gctgcggttg
    20101 cgactggtac ccccgctcga ccgagagcga gagcgcgttg ccccacggcg tgatcgcctg
    20161 cttcagccga gcctccagct ccggcgagat cgcgccgcgc gggtcaacga tgcgcagttc
    20221 attcgagcgg gaaacccagc cgaggtttgc cgcgctttcg ctgctgatca gcgcaccatt
    20281 ccattgcacg gacgcgcccc gctcgatcac cgcccaggca actggggacg ccggcacctc
    20341 gaccagctcg aactgggtgg ggcccgactc cgggatggtc gcgtaagcaa aggtgatcgc
    20401 accgttgacg atctggtgct cggcccagtt tgcttcgccg tcgcccaggt tgggatgcgt
    20461 gtcggtggcc actaatagct tgccggccgc cagttccttc cgctgggacg cgtccagtcc
    20521 gaacaactcg gtcagttcat ccaccttggc gatcaggatg ccttggccgt accagccacc
    20581 atcgctgctc agcgaggcgc aggcgccggc ttcagcgggg agatcgtcgg tggtgcagcc
    20641 cggacgcagc gccgcgaagc tctgggggtc ttcgggatcc gacccgcgtc catcggcctg
    20701 cccgacctgg taggtgcgca acaccgggtc gatatcggca atcacctgcc gcagaccggc
    20761 cgcggattca ccaccgaaat agaggctggc ctgccccagc ggcatcgacg gctggtaacg
    20821 gcgggccgcg tccgcttcga agctgagcac gagcgtccag accgtgccga gaatcagccc
    20881 gccggccagg atcgcagcca cggtggacgt tgcccggccg cgctgccggg aggtgtcccg
    20941 aagcgccatt cggacgccca tcggcgcacc gccggccagc cgcgcggcta ccccaagcaa
    21001 tgccgggacg cagagcagca ccccgatcag caccgccaat gcggaaaccg tccagagcag
    21061 gaaccgccat tccggagcca ccagcatcgc cagccacacg ccgaccagcc cggccccgac
    21121 cagcaccccg ccgagcaccg gcgcgccctt gcgcacgggt gcggaacgga ggctgccgcg
    21181 caggctgcca accagatcga gccgggccaa tcctcgggcg gtgaccagtg ccgcggcgac
    21241 ggcggtcagg gtgccgagcg ccagcacgcc ggccaactgc gtccacggca cttccagcgg
    21301 accgtgcagt tcggtgggct cggacgccat tcgctgccac accaggaccc cgaccccagc
    21361 gcccagcgca gcgccgagcg tcgccccgga aacgcccagc agcagcgcct ggcccagcgc
    21421 cgagcggcgc aactgcgcga tgctcgcccc gttggacgcg gccagcgcca ggccgcgccg
    21481 ctgccgggcg gcaccgatgg cgaacgccgg gccaaccacc agcgcgactt cgatcatcgc
    21541 ggccaggatc accatccagg tcagaaaggg cccgccgagt tcactggggt ggaactcctg
    21601 gccgctcggc ggatcggcgg cgatgccacg actggcggtc tcgaaaccgt agccggccag
    21661 gttctgcgcc tcggtccagg ccagcggggt gccggcggcc acgaagaagc tgatccgatc
    21721 ggcgtcagct tcgggcaggc cgaccaactc gaccaggccg tcgaggtgcg cgtccgcgat
    21781 gccgacgatg gtgaccgtcc gggcaacgcc gtcgcgcacc agcgtcacgg tcccggacgc
    21841 gggtagcccg gccgccatcc cgttcggtgt gaccaacgcc tcgctcgacg tctgcgggtt
    21901 gcggcccgag atgagcttga acatggactc gcccgactct ggatcgatcc cgagcagggt
    21961 ggtagtcacc tgacccttgc cgaattcgag gtaggtctcg gtgaccgcga ccgccgggcg
    22021 tccgatcagt gcggcgaccg cgtcctgatg ctcggcgacg gtgttgcccc agccgggaat
    22081 gggccgaggc tcgacgtctt tgccaccgct ggacgccgtg ccgtagccgt cccaggaggc
    22141 ctggaaaccg tacccaacct gggtgagcac actgtgcgcg gtcccaagct tgaggtcgag
    22201 ccgctcggca gccgagatgg tgttggacgc gaccaccacc agcagcgcag cgatgacgcc
    22261 aatcggaagg acaatcatca gccacacgaa caagctgcgc ccccagtcgc gacggacgtc
    22321 gcggcgcgcc atccgcaggg ccatcgccca gccgttgcgc cattgccgca gccgactcgt
    22381 ccgcacctcg gcgcggcggg ccgcgctcat cggatcgctc gggcgggttc gcggacggcg
    22441 ctgccgtcta cgactacacc gtcgcgaagg tagaccaccc ggtcggccca ggcggcgtgg
    22501 cgcggttcgt gggtgaccag cagcccggct gcgcccgcgt cgcaccgcgc gcgcagcacc
    22561 gacaggacgt cttcaccggt gaccgaatcc agcgcgccgg tcggctcatc ggccagcacc
    22621 aaccgacgct ccccgatcag cgcccgcgcg atcgcgaccc gctgctgctg gccgccggaa
    22681 agctggtcgg ggaagcggtc agcgagcctg cccaggccga gttccttgag caccgcgcgg
    22741 gcctcctgcc gggccgcgac gacgtgttgg ccgtccaact cgagtggcag tccgatgttc
    22801 tcggcagcgg tgagggacgg gatcaggttg tagtcctgga acaccacgcc cacgcgacgg
    22861 cggcgcagtc gcgccaactc gtccggcttc agcgcagaga gccgctgacc cgagatctcc
    22921 acctcgccgc tagtcggacg gtcgagcgcg ccggccaggt tgagcagcgt ggacttcccg
    22981 ctgcccgaag gtcccatcac cgccaccaac tcgccgggat ggacatctag cgacacttcg
    23041 cgcagcgcat ggacggcggt ctcgccgctg ccgtgcaccc gggtgacctc agtgagccgc
    23101 aggatcggcg cgctcatgct cgggcctcgt tgtcgtcgcg gcttgagtcg gccggttgcg
    23161 tgatcagatc ccggcctgcg ccgggataac ggtggcggac gtccgcttgc tcctcgacct
    23221 cgacgaacgg cgcgggacgg ccgtatcggg cgacactggc ctcgacctgg tcgagccaac
    23281 ggatctcggt ttcggcggcg aacagctggt gctcgacgac gagcagccag gcgagatcgg
    23341 tagcagggtc aaccgattgc ttcagccggg tgagctgttg caggcgatgc atggtagcgg
    23401 cacgctgcgt ctgggcgacc tcggcggaat ccacaccagg gctcgtgatc gcgatggcaa
    23461 gtttgatcac gagttcctcc cgcggagtca cctcgcgatc gaccggagat cgccaccacc
    23521 gttcgaccgc ttcgagcccg gcgttggtga gccggtaggc gatccgaccc tcgacatcgg
    23581 gctcgccggc cacgaccaga ccgtcgcgtt ccagccggtc gagggtggtg tagacctgtc
    23641 cgacgttcac cggccaggtg ccgccggtgc gcgcctcgaa agccgacttc agcgcggcgc
    23701 cataactcgc accctcggca agcaacgcca gcagtccgta cttgaccgac atgtccgctc
    23761 ctacatactc agtattagcc ctcgcaggag aatgtatacc gagtatgtgt gggccgcgtg
    23821 caggtggctc actcgctgga acccacctcg gctaaccgga aagacctgcg gaaacgccgg
    23881 cggacgcagt cgcgccggat tcatcgactg gtcttagtac gcgcacgctc gactggcgga
    23941 cgccggctcg agcggcccgg ctagcgggct tcggggtcgg gctgatggac gccgaacacg
    24001 ttgccctcgg tgtcgaggaa gtagccctgc cacgccatgc ccggaagggc cgccttcggc
    24061 acggccacgg tgccaccggc ggcgaggatc ttgcccgagg tctcgtcgaa atcgcccgag
    24121 ccgagggtga ggacgccgcc ctggacgggc gcgcccggag ccgggttcgg gccctggcgc
    24181 tgcatgatcg ccccgttgat cccgggctcg tcctcggtgc cggtggtgcc gccgaactat
    24241 ggcatgccgg cgtggtcgct ccagtcgtcg gtcgtccaat cgaacaccgc ctggtagaag
    24301 gcgaccgctc gcgggacgta ttcggcatgg atctcgaagt ggatcaccct gctcatctgg
    24361 aagccccgcc tgatcggcca gcgccatcac cggcaccaac cctgctgacg acacaagatt
    24421 cggatcatcg aacgcaaccc tggtcgcctt cagactgtga gaaaacaaca cccgacgcat
    24481 gcccttcgtg accgatcgat ttggaaccct agacaagtcc aacctcccag tcacaagggc
    24541 atccgcactt cgcgccacgc ccaacccacc cacctgatcg gtggatcgag ctaagcccag
    24601 atccaccgat cgcgctgaga gatcccggcc tccgccggga tgacggtgga acagcgccga
    24661 gtttgacgaa gcgacaagct cgaccatccg aatcggtgga tccgggctac ggcgcgggga
    24721 agacgtccgg atcgggttcc ttcaccagcg gcggatccgg cacctgcggt ggcttcggct
    24781 cgtccggctg gtcccgatcg tggtcgcccc aaccaccaca accccattga tggtgaccgg
    24841 gccgctcgca ttgtccaccg ccaccaccgc cgggatcgag ttcgggatcc ggctcagttg
    24901 tccctccggg ctccgtaggg gacggactcg gttccgtggg cgaaggggtg ggttcggtgg
    24961 gcggcacggt gggttccgtg ggcgacgggc tggggctctc gctcggcggc gatggttccg
    25021 acggggtggg cgaaggctcc agttcgatct cggggctcgg cgtgaccacc ggctccgtgg
    25081 gcacggacgg gctggccggc ggcgtgggcg gcactgccgc ggtcggcgcc gtggacgtcg
    25141 ggaccacggc gggcggagtt gcggacgggg tcggcgagac cttgggttcg ggcttgggct
    25201 tctccccgtc cgacttgcgc tcgctggccg aactcgaccc ggcggtgggc gtcgggctcg
    25261 cggtcggcac cgccggagtc ggatccggat gcggctcgga gctgacgggc ggagccggat
    25321 cggtcttgtc cggaggcggc ggcacgctca ccttcgccgg cggctcaacc gccggtggga
    25381 cgactgccgc agccgcggcc gcagcctcgc tcgaggaacc cctccatacc ccggaggcgg
    25441 ctgtggccgg gacggccagc aacaacacca gcaccaacgc cgcggcgatc cctacggtcg
    25501 ggttggacgt ggtcaggttc ttcagcttcg cgaacccggt gaccggcacc gcaccggcgc
    25561 ccgcggtggc ggacagcgct gccgccagcg cgatcccgct cgaacccgcc ccgaccaggg
    25621 cgatcccaag ggtcgacccg atccgggccg ccctcgccgc gatcccggcg caccttcggc
    25681 aatcgtccag gtgtccctga acccgttcag cgtggttggc gctgagtccg gagcggacga
    25741 aggcgccgat ccgcgtcagc gtccagcgac attccggatt ggcggattcg gcgggcacct
    25801 gggtctggat ccaggcctgg cgcaggcctt cgcgcgcccg gtagcagagt gccgccgcgg
    25861 cattgtcaga aaggccggtc aactcggcga tctcggacgc gctgcgcccc tcgacctccg
    25921 accaccacaa cgcttcctgc cagcgggtgg gcagcgaggc gaaggtgcgc tgaatgagct
    25981 cgcgattcgc cagccccatc aggatcgggt cgggatcgac ctcctcgatg tattcctcga
    26041 tgctgtcggt ggccgactcg tgcgacctcc gtccccaggt gcgcgcggta ttccgcagcg
    26101 tggtcagcag gtagggccgg aaggcgtcgt tcggcccacc accgttactg atcgccaggt
    26161 agacccgggc gaaggattcg gcgaccagat catcggggtc gacggagttg gtgaacgacc
    26221 gagccacgcc gccggccatc tttgcgtgcc gccgccagag cacgccgaac gcttcacggt
    26281 tgccggcgcg cgtctgcgct accagcccgg catcatccag atcctcgtcc ccgcggacga
    26341 caaggtccgg cacttcacgg gacggattca agttcgagac ccccacttga ggccggcgcc
    26401 cattccccaa tgaacagccg acaccacagg tgagtcccaa tcctaaacca acgagcgctc
    26461 cggcccaaag aggggctgac cgggtcgcgc accaggtcag cccctctccg agaattcgcc
    26521 gccgtcccct cccaggcagc ggcgtcatcc cgctgcagcc agaatccaac ctggctacat
    26581 aggaatagag gtgacaatcg ccggttcatg acgccatttc cgaagacgtc ctcggattcg
    26641 gtgccggggc agccttgggt tactgctgcc ttcgtcgttc tggctgtcgg gcccgctggc
    26701 gcggcgtagg ctctgccgat gaccgagaga cggacgaaaa ctgcagaccg cgtaccgcac
    26761 ctgccgcccg tccgcttggg ccaactgacc gaaggcaccg ctgaccagtt cgccggcgac
    26821 gaggacctgg acgggttcag tttcgccgga ctccgcttcg aggagctggt ttggacgggc
    26881 cgtcggcggc tcggctccag ccgtgtggac ggcgcgaccg cgcgcagctg gcgagcgccc
    26941 ggggccctgt tcagcggatc ggtcctcacc gggctggacg tgctcaccct aactgcggac
    27001 ggctcgggct ggcgacaggt ggaactgctg gagagccggg tcggctcact ggaactcttc
    27061 gggagcacgc ttcgatccgt ccacttcatc ggctgccgga tcggctacgc caacctgcgc
    27121 gacacccacc taaccgacgt cgcgttcact gactgcgtga tcgacgacct cgacctgatg
    27181 cgcgccactg ccacgcgcgt cgcgctcacg ggcacccggc tgcggcggct ggaactgacc
    27241 ggcgccaagc tggccgactt cgacctgcgc ggcgctcagc tcgccgaggt gagcggtatc
    27301 gagggcctcc gcggcgcgat catctcgacc gaacaactag tcgatctggc gccgatcctg
    27361 gccgcccgcc tcggcatcat cgtgccctaa ccccttcagg ggaccaagtg gccggcagcc
    27421 cggtacagct cgtaccactc cggacgggtc aactcgacgt cggcgccaag ggcagcagcg
    27481 gccacccgct cgggcgtggt ggtgccgagc accacctgga tcctggccgg atggcgggtg
    27541 atccaggcga ccgcgatcgc ttcggtcggc acctcgtact tggccgctag ccggtcgatc
    27601 accgtgttca actcggggaa gccgcggttg cccaggaaga cgccattgaa gaaccccgcc
    27661 tggaacggtg accaggcctg gacggtgagg tcgtgcaggc ggcagtagtc gagtacgccg
    27721 aggtcgcggc tgatcgactg gtccaggtcg gccatgttca tcgcgacgcc ctgggccacg
    27781 atcggcgcgt gcgtgatcga taactgcaac tggttggcca ccaagggctg ccggacgtgg
    27841 cggcgcagca gctcgatctg gttgggggtg tgattggaga caccgaagtg ccgcaccttg
    27901 cctgcgtccg ccagttcgtc gaacgcgcgg gcgacctcct cgggttcgac caacgcgtcc
    27961 ggacggtgca gcagcaggac atcaaggtag tcggtgcgca gcgcagccag cgaaccctcg
    28021 acggcgtcga tgatgtgcgc gtaggagaag tcgaaatacg gaccgttctt cacaatgccg
    28081 gccttgctct gcagcacgat gctctcgcgc tcggccgagc tgagctgcag cgcctcggcg
    28141 aagcgggtct cgcagccgtg cgcgtccccg tccccgtaga tgtcggcgtg gtcgaagtag
    28201 ctgatcccgg acgcgagcgc ggcctcgtac caggcgcgca cttccgcgtc cgacttgtcc
    28261 tggatgcgca tcacgccggc caccacagcc ggaacctgca actcggtgct gcctagggcg
    28321 aacgtcttca tggggctcct cgcgggctgt ggactacttg aacggaaaag gaaccgtccc
    28381 ctttccgttc accttcctac ccccggacgg cacagcgcaa caatcctcag ccaggcttgc
    28441 gggccacaaa ggtgtgcagg atgccgcgtt gccagccgga cgcggtgcga tgcgcgactt
    28501 ccgcgaaccc tgccgcctcg agccgatcac cgaaccgttg ccgatcatcg aagtccagca
    28561 cgctgcgcca caggtagcgg tagagcccag ggttgcggtc ggcgaccaga ccgagcggaa
    28621 tgatcaccag ccagctgacc aggtgccagc gcagcgtggc ccaacgatcg ccggccaccg
    28681 agtagtcctg caccaccacc cagccgccgg gcgcgagcag ttcgtacacc tcggccagcg
    28741 cgcggtcgcg tccggcttcg gggacgttgc ggaacaggta ggcggtgaac accccgtccc
    28801 aggagcccgg ctcgaggtcg gctacgtcga gtgcgccggc cacggcttcg gcgaagcgga
    28861 cgccgtccgg ccagctcttg cggatcgcct gggcgagcat gcctgcggac gcgtccagtc
    28921 cgaggacgga cggctgcccc ggcagagcag cgagcagagc ccgggtggag gcgccggagc
    28981 cgcaggccag atcgatgacc ttcggcgcac cgctcggccc gatgcgcccg gccagggcgc
    29041 ccgccgcacg gcgcaactcg gcgtgataac ccgggttcag tccgacgagc aggtcatagc
    29101 gggacgcgcc ccggtcgaac atccgcgaca gggccgaagg ctgcatccgc agatgctagc
    29161 ggtaatcgcc accggaaggt cgctcaggct ccgacaggtt cagccaacag ttggcggtca
    29221 ggcctcagga gtgggctcct tcaccaccat cgcgaggccg ccgaaagccg gcagatcgac
    29281 ggtgaacccg ccaagatcgt ccacggacgc aaccttgcgg cgggtggcca ggtcgatcac
    29341 gcgtccgacc gggatgtgct cggactgcac ccgtccgctg atcttctccg cgccgaagtt
    29401 cagcgcagtg atctggatcg ctcccgactc cagccggttg accagcacca gcatgctctt
    29461 cactgcgacc tccggcacgt ccagcagggt gccggtggcg aggccgtggc gcttgcggat
    29521 cttcaggatg cccgccaacc gggacgcgaa cgacgtcggg ctggccagct gatcgggcag
    29581 gggtccgtac aacgcggtgg cgatcggcat ccccgagctc gaccgcttgg cgtccggctg
    29641 ctcgccgagc aggtcgtagg cgccgcgctc gatccagcgc gtgtcgccgc cgctgatcag
    29701 ccttcgcacc ttggctcgat ccagcggcaa cgccccaacc agatcccagc ccgacagcgc
    29761 gaacaccccc ggctgccagg cgttgtacat cgacagcaac aggtgggcgt tggcgatgcg
    29821 gcgcgcgtcc gcgtccgtga tcgtctcgag atcggtgatg ccgaggctgg cggcgatgac
    29881 cgaagccgtg gtgcacgcga tgccgttctg cacgaaggtc gcgttgtagg gggccgaatc
    29941 cccggtcagc ttcgcgcgca tcgcctcgcg gacgtgttcg gccagttcgg cgccggtgta
    30001 gtccacacca ccaagggtgt aggcatcgtc gcggtgccgc gcggcgaaat ggacgagttc
    30061 gtaggtcagc tcgtcgtggt tctgcagcgc gtgcacgagg gacgcctggt cgatcccgag
    30121 ctgcagggcc tcacgcaggg ttagccgcag gaactcggtg tcgccggtgc agagcgcgaa
    30181 ctggtaggcc ggacgggtca cgaagtcgta ggagaggtcg gggccgaccg cgctggtgtg
    30241 caggatgtcg tccatcgcaa ggttcagctc ctggaaggtg aacccgccca ccttgcgcac
    30301 catcgacccg atcaactggt tcgcagcctg cgagagcgga tgcccctccg accagcccgg
    30361 cgcctggtcg agtgatttct ccactccaag gaagccgttg gcgtcgagtc gcaggccacc
    30421 cgaaccgagg tcgagcagcg agtgcagcgc gtcccccatc accaggcgca tgccggcgaa
    30481 agtcgggtcg agccagttga tggacggctg gccctccttg aagtagtgca ggtagaccca
    30541 gcgacgctgg acacccttcc agtcatagat cggcggggtc accgaccagt tggtctcctt
    30601 gaccccgggt tcgtagaaga tcacccgctg caattcaccg atgatgtaac ccgcctcagc
    30661 caacgcacgc tcggcgtcgg cggtcaggtt gaccgagtcc aggccttcgg ggacgtcggg
    30721 cagcagatcc cacgcctcct cggggatgtt gaccatgtgg tagatgcctg ggtagtcaag
    30781 gtaattcagt tcggccagcc ggaagtcggc gcccttgccg gtgtgcgctg gaacgatgtc
    30841 gtcgatgatg gtgccacgtc ggcgcgcagc gttcgcacac atctctcgga attcatcctc
    30901 ggtgccgaac actggatcga tctgcatgct gatccgatca aagtgaccgt ccacgctcgg
    30961 tgtgggcaac caacccgaga tgccaccggc aagcttgagc ggcccggtgt ggatcgcgtc
    31021 gatgccgatc tcggcgaacg cggcccacag ctcctgcgcg ccgagcgcgc cgaggaagga
    31081 ctgccccggg cgggtgatca gcgagagcgg gtaggccgtg aaccaaaccg acgcctgctg
    31141 aacggctgct cgcggatccg gatgcgcgta cgggctggcc cacatctcat ggtgaccggc
    31201 cagctggcgg ccgagcaccg tcgcatcgtg cagcatcgac tgctgctcca gccaggcgat
    31261 gtaggccggg ttgcgtccgt
//
LOCUS       DAOPRQ010000026        30800 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_1164_length_30800_cov_12.123012, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000026 DAOPRQ010000000
VERSION     DAOPRQ010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 30800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30800
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1164_length_30800_cov_12.123012"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="PKJ61_05000"
     CDS             <1..411
                     /locus_tag="PKJ61_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13214.1"
                     /translation="MRRDAIITAGALSLGVGLALLAIGAWLQAVPVAILLIGWIFAGL
                     GMGLAVASTSLGVMQLSPAVELGRSTSSLQVGEALGNSLGAGLAGTLYLLAAGVLPTA
                     LGFAPLLTAMAGLAVLGTLASRRIGELANHSTAA"
     gene            769..1257
                     /locus_tag="PKJ61_05005"
     CDS             769..1257
                     /locus_tag="PKJ61_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012923277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOC13215.1"
                     /translation="MDEVDRILAAWSRELPEVDVSPLGSLSRVSRLAKHLDRARAEAF
                     AAHELEVWEFDVLAALRRQGRPFVLSPGELIHETLSTSGTMTNRINRLEARDLVVREP
                     NPDDGRGVRVRLTSKGRDCVEAALADLLAYERRVLAAIEPPERAELAGVLARLLAPFE
                     RS"
     gene            1328..>2092
                     /locus_tag="PKJ61_05010"
     CDS             1328..>2092
                     /locus_tag="PKJ61_05010"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HOC13216.1"
                     /translation="MALSSSVLAELAAALGIATEFWDWKGRLTEITDETVIAILAGMD
                     VDASTPASAQAALDAHRLRPWTRLLPPCVVMEQGTAKTVQVHLPHGDWVRVSVRLEEG
                     GWRDAEQVADDEPPRLVDGVLTGQASFALPADLPLGYHRLQATTAAGHSESTLVVSPA
                     FLGFPASMGEKRTWGYAAQLYSIRSADSWGMGDLLDLADLATWSATQQFAGFVLVNPL
                     HAAQPMPPLEPSPYLPTSRRYVNPLYIRPEAVPEYAE"
     assembly_gap    2093..2192
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2193..3648
                     /gene="malQ"
                     /locus_tag="PKJ61_05015"
     CDS             <2193..3648
                     /gene="malQ"
                     /locus_tag="PKJ61_05015"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HOC13217.1"
                     /translation="RYVNPLYIRPEAVPEYAELDERSRTRIAQRKAQLRKHLAGETFI
                     QRDLIWAAKIDALQVVYAHGLRPARRMAFNDFLRREGRGLTQFATWSALVNSYGDDWR
                     EWPAELRRPSSPEVAVFAEEHLDLIIFFAWLQWIADDQLRAAQSAATDAGMRVGIMND
                     LAVGVGGDSAEAWTYSNLFADGISVGAPPDHFNQTGQDWGQAPWRPDRMDDLSYAPFR
                     SMVSGILRHSGGIRVDHIMGLFRLWWIPDGMPPSQGTYVRYNHEAMVGILALEAHRAG
                     ALVVGEDLGVVEPWVRDYLRERGILGTSIAWFERDSAGRPLPPEAWREYCLASVTTHD
                     LPPTAGYLAAEHVRLQHRLGLLTEPLDAELANAERDRNAYLDMLRERGFLAVGEPTTE
                     DIVEALHRYLVATPARVLCAALTDAVGDRRTQNQPGTSTEYPNWKIPLSGPDGDPLTL
                     EDLYQDERAFRLASVMNDFSSPLQVPGSGTSLVV"
     gene            complement(3724..4326)
                     /locus_tag="PKJ61_05020"
     CDS             complement(3724..4326)
                     /locus_tag="PKJ61_05020"
                     /inference="COORDINATES: protein motif:HMM:NF018749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1349 domain-containing protein"
                     /protein_id="HOC13218.1"
                     /translation="MRLVRGEPDGTRRVAWSEGVWTNPPAAVTTAEKALEVTAVEGSD
                     AWRHTAYGFVHDTEHALLAPLAVGEAVEVVLQTTFSGQFDQAGVLVRADAEHWVKAGC
                     EYADGALNLGAVVTLERSDWSVAPVDWNGQEITVRVSRDAESLTVRAKVAGGDFRLVR
                     VAPFVTGEVSAGPYVCAPTRPDFTVRFLSWRVVPGDASLH"
     gene            complement(4324..4908)
                     /locus_tag="PKJ61_05025"
                     /pseudo
     CDS             complement(4324..4908)
                     /locus_tag="PKJ61_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016455201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
     gene            complement(4905..6293)
                     /locus_tag="PKJ61_05030"
     CDS             complement(4905..6293)
                     /locus_tag="PKJ61_05030"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HOC13219.1"
                     /translation="MTLLSSVSPSTLRSGQLSTALLTDHYELTMVRAALRSKTALRRS
                     VFELFARRLPEGRRYGIVAGAGRAMDAIQAFRFDEEQLNFLLEQNVIDTDLADWLSRY
                     KFKGNIWGYPDGEAYFPGSPLLIVEGTFAEAVILETVLLSIYNYDCAVASAASRMTAM
                     AGDRPCIEMGSRRTNESAAVAAARAAYIAGFQATSNLEAGRTYGIPTTGTAAHSFTLL
                     HDTEEDAFKAQLKTLGKGTTLLVDTYDIEEAVRTGVRLSKGKLGAIRLDSGDLAEQAA
                     DVRKLLDSLGAEKTKIIVTSDLDEFQIAALRSAPVDGYGVGTSLVTGSGAPTCGFVYK
                     LVSRATSEAKDAELLPVAKKSVNKGSVGGRKFALRRLNAAGRAEAEVIGIGTPPVNDG
                     NDRPLLVQLMAKGKPIAREPLEAARERHTRVRAELPLAAHKMSKGDPALPTILLDEVG
                     EVTDNPYAAVKR"
     gene            complement(6970..7242)
                     /locus_tag="PKJ61_05035"
     CDS             complement(6970..7242)
                     /locus_tag="PKJ61_05035"
                     /inference="COORDINATES: protein motif:HMM:NF020265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HOC13220.1"
                     /translation="MAGGTKTRRLEARLEPTLDDLISEAARTVGQSRSEFILIAVRES
                     AERVLARADITIMSAHQFDVLVESLAERAPLDKLARAAASDRPYRR"
     gene            7426..7689
                     /gene="clpS"
                     /locus_tag="PKJ61_05040"
     CDS             7426..7689
                     /gene="clpS"
                     /locus_tag="PKJ61_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HOC13221.1"
                     /translation="MAVSERPVESALTGWVTIVWDDPVNLMSYVTHVFTTYFGYPESK
                     ATDLMLQVHNSGKAVVSTGAREQMETDVDAMHGYGLWATLEPV"
     gene            7689..8243
                     /locus_tag="PKJ61_05045"
     CDS             7689..8243
                     /locus_tag="PKJ61_05045"
                     /inference="COORDINATES: protein motif:HMM:NF020989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2017 family protein"
                     /protein_id="HOC13222.1"
                     /translation="MRDFRRQGPVINMRLTDYEATLLESLVEQFCGLLEADAEAVDHA
                     DPFARWQAELDGGEPLDRTDPVIVRLFPDAYPEDPIGSDEFRRLTQSRQRTERLDQAE
                     IVMSALRDSDAGKHPVQVRLIDLPSWLKTLTAVRLSLAVRLGIRTSEDAEELDELPET
                     DPKAYVFRVYEWVAYLTENLLALA"
     gene            8311..8541
                     /locus_tag="PKJ61_05050"
     CDS             8311..8541
                     /locus_tag="PKJ61_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13223.1"
                     /translation="MYVGIDLGSAPLHTSLTLVGAPDGSRQRLSCLGLRVGSTFSLLS
                     RTAGGGRVAMVSGSRIALGSSLIKELRAEVVA"
     gene            8541..10586
                     /gene="feoB"
                     /locus_tag="PKJ61_05055"
     CDS             8541..10586
                     /gene="feoB"
                     /locus_tag="PKJ61_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015071061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HOC13224.1"
                     /translation="MPVTETKPNRQLNLLVASVPTPSVKACCQTGTGTGAGPDAPAVA
                     LVGAPNTGKSTLFNALTGSRVTMGNWPGTTVEVSRGVWKTTRDAATCDCEDCTCDESD
                     QKLDITLVDLPGAYSLDPHSPDEALTRELLVGVPADERPDLCIVVCDAARLANSLYLV
                     SQLRELGLRLVVALTMLDVAAKNGVSVDTAALSKALGCPVVGIDPRRRSGLEGLAGTV
                     RETLGTPAPAPRPMAAELDSLELDDDRFSWIAQAVEVGTKAPADPQHTWSDKVDRWVT
                     APVLGPAIFLGVMWLVFQITTAVAAPLQDFLDGLFTGPLTDGALWLFAQLGLTGTWLE
                     GLVVNGIIAGVGMLLTFVPLMTLMFVLLALLEDSGYLARAAVVTDRLMRAIGLPGRAF
                     LPLIVGFGCNVPAISATRILPSSRQRILTALLVPFTSCTARLTVFVMLGSVFFGPYAG
                     TAVFAMYVISILLVVFTGLILRRTLWRTMGAEPLIIDLPAYQRPTLRLTASVTWVRLK
                     GFLQTAAGVIVITVCAVWLLQSIPTQPGERFGDVPVADSVYGVAAQAVAPVFGPAGFG
                     SWETTSTLVVGFVAKEAVISSWAQTYAVEEPEDSAAPGDLGDAVLASFTESSGGHPVP
                     AALAFMIFLLAYTPCVATVAAQWREIGGRWTLFGIGLQLAIAWGLAVVVFQVGSLFL"
     gene            10583..10888
                     /locus_tag="PKJ61_05060"
     CDS             10583..10888
                     /locus_tag="PKJ61_05060"
                     /inference="COORDINATES: protein motif:HMM:NF020581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoC-like transcriptional regulator"
                     /protein_id="HOC13225.1"
                     /translation="MTAAAGVRRGPLSLVLDEMAVGTPTVAQMARNLALDEQVVRAAV
                     DHLVRSGRVSARELSMGCPASGCGGCASAVDGEPGCGLPAPVPGRRAGLVTLTLTRR"
     gene            complement(11015..11176)
                     /locus_tag="PKJ61_05065"
     CDS             complement(11015..11176)
                     /locus_tag="PKJ61_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13226.1"
                     /translation="MADQIDAARIVLRTLRWLDDGRRKLDESWRAFTGREEKPAAAPA
                     DRREPPEIW"
     gene            11236..12042
                     /gene="murI"
                     /locus_tag="PKJ61_05070"
     CDS             11236..12042
                     /gene="murI"
                     /locus_tag="PKJ61_05070"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HOC13227.1"
                     /translation="MIASDAPIGVFDSGFGGLTVARALLDQLPGEDMVYLGDTARAPY
                     GTKSIPEVREYALECLDYLAESGVKALVIACNSASSAVLRDARERYDVPVIDVILPAA
                     RRAVRASKSGRIGLICTEATATSASYDDALAAVPGVQLLTAPCPLFVEYVEAGITGGP
                     ELLDAARGYLLPLREAGIDTLILGCTHYPLLAGVISYVIGDEVTLVSSAEECAKEVFA
                     TLTDTGLVRSAPRTPTLRFLTTGSPELFAGIGRRLMGGFVDEVEQVYTIA"
     gene            12155..12313
                     /locus_tag="PKJ61_05075"
     CDS             12155..12313
                     /locus_tag="PKJ61_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13228.1"
                     /translation="MTRFRPTIVRDGTLASFTGVVGADRVISREGPGKAIICEIYRWV
                     GESRRGVS"
     gene            complement(12490..12945)
                     /locus_tag="PKJ61_05080"
     CDS             complement(12490..12945)
                     /locus_tag="PKJ61_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007078862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOC13229.1"
                     /translation="MSSRRFTGRLAAAAVTLLASAALLSGCSSTSVSVSAGPTATAAP
                     AAGSSLDASAFAAAARLPGTVLLDVRTAEEFAAGHLPGAVNIDVESPDFAAGIASLDP
                     SKPYAVYCRSANRSKVAMTAMQGVGFTNLYDLAGGINAWKSAGGEVVTG"
     gene            complement(12942..13148)
                     /locus_tag="PKJ61_05085"
     CDS             complement(12942..13148)
                     /locus_tag="PKJ61_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13230.1"
                     /translation="MLGSSFENGDCEMCRPVTCKVCGKTTWAGCGQHVAQVKAMVPAN
                     QWCGGHAAATAPEKSSGLFKIFGR"
     gene            13233..13958
                     /gene="rph"
                     /locus_tag="PKJ61_05090"
     CDS             13233..13958
                     /gene="rph"
                     /locus_tag="PKJ61_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016669555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HOC13231.1"
                     /translation="MSTPRADGRSPLDLREVRITRNWLDHAEGSVLVEFGATRVLIAA
                     SVTEGVPRWRKGSGLGWVTSEYAMLPRATHSRSDRESVKGRIGGRTHEISRLIGRSLR
                     SVIDYRALGENTIVLDCDVLQADGGTRTASITGAYVALVDAVTWLRNRGALAGEPLTG
                     SVAAVSVGVVDGTPMLDLCYPEDSSAEVDLNVVATGGGEFVEVQGTAEGKPFARPVLD
                     ELLDLGLAGCAELTRLQQAALAR"
     gene            13955..14557
                     /gene="rdgB"
                     /locus_tag="PKJ61_05095"
                     /pseudo
     CDS             13955..14557
                     /gene="rdgB"
                     /locus_tag="PKJ61_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002523591.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
     gene            14695..15489
                     /locus_tag="PKJ61_05100"
     CDS             14695..15489
                     /locus_tag="PKJ61_05100"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020392040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HOC13232.1"
                     /translation="MQQYLDLLQHVLDHGTAKSDRTGTGTRSVFGYQMRFDLAVGFPL
                     LTTKRLHTRSIFGELLWFLRGSTNVEWLHDNGITIWDEWADANGDLGPIYGYQWRSWP
                     TPDGGHVDQIKGVIESIQNFPDSRRHIVSAWNVGQLGEMALPPCHAFFQFYVADGKLS
                     CQLYQRSADIFLGVPFNIASYALLTHLVAQVTGLEVGDFVHTLGDAHLYSNHLEQAKL
                     QLTREPRPLPRLLVDPSVTEIDAFTLADLEVVDYDPHPGIKAPIAV"
     gene            15492..16007
                     /locus_tag="PKJ61_05105"
     CDS             15492..16007
                     /locus_tag="PKJ61_05105"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016669558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HOC13233.1"
                     /translation="MRIIAIAIVGANGVIGDGERQPFEIAEDWARFKRVTLGHPLIMG
                     RKTHDAIGRWLPKRTTIVVTSHPETIALPEPTRVVEPIETLPPRTAYAVASLDEALDL
                     AAGLDAEIYVAGGGSIYRQAWPRLTDLDLTEVHQVADGSVLFPDVRPAEWVEVSREPR
                     DGFDFVHYRRR"
     gene            16011..16652
                     /locus_tag="PKJ61_05110"
     CDS             16011..16652
                     /locus_tag="PKJ61_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13234.1"
                     /translation="MLLPARIAAGVRDGTITLAFRRWEQPRVRVGGTQLTSAGIVGFD
                     AVEEVFGAERLTDADARAAGFASLTALRRQLYPPEPPPEEVAPREPRWVGPRASKGGY
                     HVYRVRLSWVGDDPRVALRDALPSAEELTEITAAVAKLDAAKRTGGWTRPILEWIRDH
                     PGVISTELAALLQRELQPMKTDIRKLKALGLTESLRVGYRLSPRGRAYLESLD"
     gene            16655..17950
                     /locus_tag="PKJ61_05115"
     CDS             16655..17950
                     /locus_tag="PKJ61_05115"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008361831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HOC13235.1"
                     /translation="MRPQASIMHLDLDAFFAAVEQRDKPSLRGKPVIVGGIGLRGVVS
                     TASYEARKFGVGSAMAMGEARRRCPHAAFLAGRFGSYREASAIVMRLLHELSPLVEPL
                     SLDEAFVDLAAGPERDLDDDSLLALAQDLRARLADATGGLTASVGLGSSKFIAKVASE
                     LAKPNGVRLVAPGTEVATIAELPARAIPGVGPATMDRLSRLGVSTVADLQRLSRAELV
                     RELGKSGGEHLSDLAWAVDDRPVEPERETKSISVEDTFERDLADADVLAGIADRQAIS
                     VVQRLTKAELFARTVTVKVRWPDFSSISRSRTLNGATDRVEVVAQVARSLLEGLDTSQ
                     GVRLLGVGVSGLVEVAQEPLFDLVDSAPPRVVEEAEAPLTRRPSGWLAGADVVHVQFG
                     QGWVWGSGLGRVTVRFETADTGPGPVRTFAADDPELSPA"
     gene            18552..18633
                     /locus_tag="PKJ61_05120"
     tRNA            18552..18633
                     /locus_tag="PKJ61_05120"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18586..18588,aa:Leu,seq:tag)
     gene            complement(18673..19758)
                     /locus_tag="PKJ61_05125"
     CDS             complement(18673..19758)
                     /locus_tag="PKJ61_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012924063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOC13236.1"
                     /translation="MPIGVGLVGAGNISTQYLTNLTTYPDLKVVAIDDLIPERARAQA
                     EKFGVDTWGGAGTVCANPEVEVVVNITPPEVHLSVSAQAIRAGKHVWSEKPLGLDRTA
                     ARTLLDEAAALGVQVCCAPDTVLGPGIQAALRSMDAGSIGRPLAGLAIMQVPGPDLWH
                     PDPAFLFQTGAGPVLDIGPYYFTSLVLGLGPVTSVVAAGGRAREVRTVVSGPKAGTEF
                     AVTVPTTANVLLRHASGASSVCLYSFDSGLARAGVLEFQGSTGTIVAPDPNMFDGTVR
                     THVGGELTGEVAIEAAGHGRGIGVVDLVRSLAEGRRPRLNGELAYHVLDIMLAVEESI
                     ASGEPVAVTSAPPAVDLLPDSWSPLSA"
     gene            complement(19758..20486)
                     /locus_tag="PKJ61_05130"
     CDS             complement(19758..20486)
                     /locus_tag="PKJ61_05130"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOC13237.1"
                     /translation="MARVSIQLYTLRDQLASDPETTLAAVAGMGVDEVEPFGIEHFDW
                     LADSLAAHGLKAGSAHAGLLDDPEKVLAAATALGVRTVFQPYWEPVQWTSADGIRGLA
                     EALNSQVDRFAEAGVTIGYHNHDFEFTAPDVDGVPAYDYFVSLLDERVVLEVDTYWAA
                     AGGRDVPALLAKYGSRITHLHVKDGPLLPSDSGALNAVLGTGSMDLPPILDASPDRVW
                     VVEFDLATSPLEEVGQSIGYLRER"
     gene            complement(20593..21324)
                     /locus_tag="PKJ61_05135"
     CDS             complement(20593..21324)
                     /locus_tag="PKJ61_05135"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOC13238.1"
                     /translation="MARVFITGSSDGLGHAAAATLLAVGHEVVVHVRNQSRITAVQGL
                     IAQGAKAVVGDLSDLTATMDVAEQVNRLGRMDAVIHNAGVVSGPAVLPVNVVAPYLLT
                     ARIDRPGRLIYLSSGLHRGGRPDLSGLDWGGASVTATYSDSKLFVTALALAVARFWPG
                     VLSNAVDPGWVPTRMGGPGAPDDLRLGHLTQEWLATSEAAPALVSGGYWHHQRQRPPH
                     PSTADPTFQDSLLTALADHTGTVLG"
     gene            complement(21338..21919)
                     /locus_tag="PKJ61_05140"
     CDS             complement(21338..21919)
                     /locus_tag="PKJ61_05140"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOC13239.1"
                     /translation="MENVPAPARDAREAIIRATIDLIRHQGVAGTSISDIVARSKTSA
                     GAIYHHFGNKERLILEVGQSILAKPMEMVLRTSPELSPTELFRAALAQMARDETTPEL
                     LLQIWAGAKSDAKLGRLIHSQAMVARSAVRTFMANWCAEHSSDTDPDDLVDLIMGLVA
                     GYAALRAMSVNRDPEAYGELGSRLIAQALATGL"
     gene            22031..26374
                     /locus_tag="PKJ61_05145"
     CDS             22031..26374
                     /locus_tag="PKJ61_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002548511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HOC13240.1"
                     /translation="MPQQFALGLDIGSTTVKAVVLNAGTVRFSAYRRHNADARGALRT
                     LLADVAQRYPGQQFRCAVTGSAGLGVADLLGVEFVQEVIASTAAIERFIPDADVVIEL
                     GGEDAKITFLRPVVEQRMNGTCAGGTGAFIDQMATLLHTDAGGLDDLAAKHKHLYPIA
                     SRCGVFAKSDLQPLLNQGAAHTDLAASVFAAVATQTIAGLACGHPIRGNLVFLGGPLH
                     FLPQLRRAYERALATQVDSFTTPDEAHLYVAIGAALLTGSGSGVAIEDLIGRLRERTL
                     IPLASTRMRPLFKDQAERDEFDVRHGSETIARGDLSAASGPMFLGIDAGSTTVKAVVL
                     TASGELVFSHYQGSNDPVGVALSILRTVLTSLPADAYLARACVTGYGEGLVRAALRLD
                     DGEIETMAHYRAAERIAPGVTSVIDIGGQDMKYLRIRNGAVDSIAVNEACSSGCGSFL
                     QTFAASMDTDVAAFASMALESSAPVDLGTRCTVFMNSSVKQAQREGAAVGDIAAGLSY
                     SVVRNALYKVIKLKDASQLGELVVVQGGTFLNDAVLRAFELQTGAQVIRPDIAGLMGA
                     YGAALTAQRNWSDGERSQVLGLDELDEFRVDTHLDTCRLCQNHCQLTISTFGDGTRHV
                     SGNRCERGASTEKVPKKSELPNLYDYKYRRMFGYRRLTADLAFRGDIGIPRVLNMYEN
                     YPLWFTILTRLGFRVTLSGRSSHEVFERGMESIPAENACYPAKIAHGHIESLLDRGIN
                     TIFYPCVPLEAKEVKGADNHFNCPVVAFYPQVLQRNLVRLQEPGVRYLSPFLNLDNPE
                     KLAKRLVEVFADWDVTLAEATAAVEAGFIEDANAKADIRAEGRRALEYMTAHGVKGIV
                     LAGRPYHVDPEINHGIPSLINGLGMAVLTEDAVVEPSATALARPIRVRDQWAYHTRLY
                     ESAAVVAKQPDLHLVQLNSFGCGVDAITTDQVQEILEQAGEAYTLLKIDEVSNLGAAT
                     IRLRSMKAAAAERRGSATVSTFGPLGERRLFDENARDTHTIYAPQMAPMHFRLLMPVF
                     GKVGLNMKLLERASSDDVEVGLKYVNNDACFPAIMVVGQLVNKIRSGEADPDRTSVAI
                     TQTGGMCRATNYVGLLRKALADAGYPQVPVLAISVQGLEANPGFKITPGLIHPALQAL
                     VLGDLMQTVLLRVRPYEVVPGSAKALYDRWDRICQEFLQHSGYSATLGRSVGYTALIR
                     RLVREFDELPLREVPRKPRVGVVGEILVKFHPDANNHVVDVIEAEDCEAVIPGILQFF
                     LMPLYSAQYQWETLGIGRKSHHVKKLATWLIEKYQQPVAQALAKTGKFDVPPRMSDLA
                     KKADGVISLGTRAGEGWLLTAEMVELIEIGAPNIICAQPFACLPNHVVGKGMFAELRR
                     LHPEANIVAVDYDPGASETNQLNRIKLLVATAHKRHHAEQHIGRADSVGLGEPQLGTA
                     PVSPADVWDLV"
     gene            complement(26375..26818)
                     /locus_tag="PKJ61_05150"
     CDS             complement(26375..26818)
                     /locus_tag="PKJ61_05150"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HOC13241.1"
                     /translation="MDQDVERRHPRGPLVLRTLAMPRDTNPRGDIFGGWILSQMDIAA
                     GIMAAEIAQGRVVTVTVTDVVFHQPVAVGDTICVHAELLKVGRSSLDLKLEVWARGLV
                     AIYEEERELVAEGVFRYVAVDEDGRPRPVADNPAFFTRPTSEPNE"
     gene            26976..28775
                     /locus_tag="PKJ61_05155"
     CDS             26976..28775
                     /locus_tag="PKJ61_05155"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HOC13242.1"
                     /translation="MTDTGNEVDLSYRDASLPVAERVEILLGQMTLPEKAGLFFQTMI
                     TMGADGSLTDGDPVFGLPSTDEYVNGRLMNHFNILTAAPTPEAMATWHNRLQELAAST
                     RLGIPVTVSTDPRHSYSDNPLAGLSAGCFSVWPEPLGLAAIGDEAVVERFGDIARQEY
                     TAVGIRVALHPQIDLATEPRWSRQLQTFGEDVELTSRLGAAYIRGFQGRVLGTDSVAT
                     MTKHFPGGGPQLDGEDPHFAHGREQVYPGGQFELHLKPFEAALEAGTSQMMPYYGMPI
                     GTELEEVGFGFNKAVVTGMLRERYGFDGIVCTDWGLINDAAIMGAPFPARAWGVEHLS
                     PRERMVKVLAAGCDQFGGEACPELLIELVESGEISEERLDVSARRLLREKFVLGLFDR
                     PFVEVARASAVVGSPEFVAAGLAAQRASLTLLTNRAYGAGESVLPLGTGRKLYVEGID
                     SAVAGRYGTVVGEPGEADVAILRLQAPWEQRPTMFENFFHSGSLDFADDVVAHVREVA
                     AVVPTVLDVFLDRPAILTPFVDVVTAIVANWGASPTALLDVLTGVDQPQGSLPFDLPS
                     SMAAVEANLPDVPFDTAEPLFRFGHGLRYASAG"
     gene            complement(28954..>30800)
                     /locus_tag="PKJ61_05160"
     CDS             complement(28954..>30800)
                     /locus_tag="PKJ61_05160"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HOC13243.1"
                     /translation="RLHLELTPSGLLRSRAELANTGTDDYQLNDLVLAFPVPSTAREL
                     LDLAGRWGRERVPQRREFTVGTHLREGRKGRTGADAATVLHAGVPGFSFGDGEVWGVH
                     VGWSGNHTHYAERTFTGHQVLGGGELLLPGEIVLGPGHGYATPWLYGAYGRGLDAVAR
                     RFHRFLRSRPQHPGTKRPVTLNVWEAVYFNHDLPTLLQLAEKAAAIGVERYVLDDGWF
                     GSRRDDFSGLGDWTVSADVWPDGLHPLVNRVRELGMEFGLWVEPEMVNLDSDLARAHP
                     EWVMATGGRLPVASRHQQVLNLGIPECYDHIRDALLAILTEYDIAYLKWDHNRDLIDA
                     GTQPSGRPGVHLQTAAVYRLLDELRAAFPHLEIESCSSGGGRVDLGVLERTDRIWVSD
                     CIDPHDRQQMLRWTGQLVPPEMLGSHVASGISHTTGRSHDLNFRAATALFGHFGIEWN
                     LNTATAAELAELTEWIAFYKEHRELLLGGDVVRLDFPDAALLAGGVVSPDRSQAIYSF
                     AAMAGTDAVMLGRLTFPGLDPDRRYRVTPVQVGQAPSGLRPPLWWGLERDYTNELADL
                     HSGAGPRFVATDRNLGIELPGSVLLHTGLTDAAVNPDHALLYLVTAVD"
BASE COUNT         4764 a        10315 c        10620 g         5001 t
ORIGIN      
        1 atgcgccggg acgcgatcat caccgcgggc gcgctcagcc tcggcgtggg gctcgcgttg
       61 ctggctatcg gcgcttggct ccaggcggtg ccggtggcga tcctgctgat cggctggatc
      121 ttcgccggcc tggggatggg cttggcggtg gccagcactt cgctaggggt gatgcagctc
      181 tcgccggccg tggagttggg ccgcagcacc tcgtccctgc aggtggggga ggcgctcggc
      241 aacagcctcg gtgcaggcct ggccggcacg ctctacctgc tggccgccgg agtgctgccg
      301 actgctctcg gcttcgcgcc cctgctcacc gcgatggccg ggctggcggt cctcggtacc
      361 ctggcgagcc gtcggatcgg cgagctggcc aaccactcca cggccgcctg accgaacgag
      421 tcgaccatcc gttgctgccg cccacggcgc gccgaacaag gtggacgctt tcgcactggg
      481 acggcgagaa acggtccacc ctgggaaccg gggaggtgct cgcctcacag cgcggtgtcg
      541 tgaactcgta gcccgttgcc actggcttcg ctcagccaag tggcaccttg cgccgatccc
      601 tacgacttgg cgacatcggc gctggggacg atggcacggg catgacgcga tttggacccg
      661 aaacgaaacc ccagagcggt ggccagctat gacgagaacc tcgcgggacc aaatcgaggc
      721 agccctcggg tcggctaaag tcttgatatc gagataccag gaggtgcgat ggacgaagtc
      781 gaccgcatcc tggccgcgtg gagccgtgag ctgcccgagg tcgacgtcag cccgctgggc
      841 agcctgtccc gggtgagccg cctggccaag catctagacc gcgcccgcgc tgaggccttc
      901 gctgcgcacg agttggaggt ctgggagttc gacgtgctcg ccgcacttcg tcggcagggg
      961 cggccattcg tgctctcacc aggagagttg atccacgaga ccctttcgac ttcgggcacc
     1021 atgacgaacc ggatcaaccg gctggaggcc cgcgacctcg tggtgcgcga gcccaatccg
     1081 gacgatggcc gcggcgtccg cgtccggttg accagcaagg gccgggactg cgtggaagcc
     1141 gcgctggcag acctgctcgc gtacgaacgc cgggtgctcg ctgccatcga accgcctgag
     1201 cgggcggagc tcgccggcgt gctggccagg ctgctggcgc cattcgagag aagctgaccg
     1261 ctttcggata gggttagctg tactacctgc accaaagacc tacctgcacc aaagaaggcg
     1321 tcccggcatg gcgttgtcct cctccgtact ggccgagttg gccgctgccc tcggcatcgc
     1381 caccgagttc tgggactgga agggccggct gaccgagatc accgacgaga cggtcatcgc
     1441 gatcctcgcc ggcatggacg tggacgcgtc cacaccggct agcgctcagg ccgcactgga
     1501 cgcacaccgg ctgcggccgt ggacccggct gctgccgccg tgcgtggtga tggagcaggg
     1561 tacagccaag acagtccagg tgcacctgcc ccacggcgac tgggtgcgag tctccgtccg
     1621 cctggaagag ggtggctggc gcgatgccga gcaggtggcc gacgacgagc cgccgcgctt
     1681 ggtggacggg gtgctgacgg gccaggcgtc cttcgcgcta cccgccgacc tgccgctcgg
     1741 gtaccaccgg ctgcaggcga ccaccgcggc gggccatagc gaatccaccc ttgtggtgag
     1801 cccggcgttc ctcggtttcc cggcttcaat gggggagaag cggacgtggg gctacgccgc
     1861 gcagctgtac tcgatcaggt ccgccgactc ctggggcatg ggcgacctgc tcgacctcgc
     1921 cgacctggcc acctggtcgg cgacccagca gttcgccggt ttcgtgctgg tgaacccgct
     1981 gcacgctgcc cagccgatgc cgcccctgga accgtccccc tacctgccga cctcgcgccg
     2041 ctacgtgaac ccgctctaca tccggcccga agcggtgcct gaatacgccg agnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnccgctacg tgaacccgct ctacatccgg
     2221 cccgaagcgg tgcctgaata cgccgagttg gacgagcgct cgcgcacccg gatcgcgcag
     2281 cgcaaggcgc agcttcgcaa gcatctggcc ggcgagacct tcatccagcg ggacctgatc
     2341 tgggcggcca agatcgacgc gttgcaggtg gtctacgcgc acgggctgcg tccggcccgg
     2401 cggatggcct tcaacgactt cctgcgccgc gaaggccgcg ggctgaccca gttcgccacc
     2461 tggtcggcgc tggtgaacag ctacggcgac gactggcggg agtggccggc cgagctgcgg
     2521 cgtccgtcct cgcccgaggt ggcggtgttc gccgaagagc acttggacct gatcattttc
     2581 ttcgcctggc tgcagtggat cgccgacgac cagctgcggg ccgcgcagtc ggcggcaacg
     2641 gacgcgggca tgcgggtcgg catcatgaac gaccttgccg tgggcgtcgg cggcgacagt
     2701 gccgaggcgt ggacctactc gaacctgttc gcggacggga tcagcgtcgg cgcaccacct
     2761 gaccacttca accagaccgg ccaggactgg gggcaggcgc cgtggcgtcc ggaccgcatg
     2821 gacgacctgt cctacgctcc gttccgcagc atggtctcgg gcatcctgcg gcactccggc
     2881 ggtattcgcg tggaccacat catggggttg ttccggctgt ggtggatccc ggacggcatg
     2941 ccgccgagtc agggcaccta cgtgcgctac aaccacgagg cgatggtcgg cattctcgcc
     3001 ctggaggcgc atcgcgccgg cgcgctggtg gtcggcgaag accttggagt ggtggagccg
     3061 tgggtgcgcg actacctgcg tgagcgcggc atcctgggca cgtcgatcgc ctggttcgag
     3121 cgggactcgg ccggccgtcc gttgccgccg gaagcctggc gggagtactg cctggcttcg
     3181 gtgaccaccc acgacctgcc gccgacggcc gggtacctgg ctgccgagca cgtgcggctg
     3241 cagcaccggc tgggcctgct gaccgagccg ctggacgcgg agttggccaa cgccgaacgc
     3301 gaccgcaacg cctacctgga catgctgcgt gagcgcggct tcctggccgt gggcgagccc
     3361 accactgagg acatcgtcga ggcgctgcac cgttacctgg tggctacccc ggcgcgggtg
     3421 ctgtgcgcgg ccctcaccga tgccgtgggc gaccggcgga cgcagaatca gcccggcacg
     3481 tcgacggagt acccgaactg gaagatcccg ctgtccgggc cggacggcga cccgctgaca
     3541 ctggaggacc tctaccagga cgagcgcgcg ttccgcctgg ccagcgtgat gaacgacttc
     3601 agctccccgc tgcaggtgcc cggttctggg acttcgctgg tcgtctgatt ggcgccgtga
     3661 cgctgagact gtggacaact tgaacgaaaa gggaaccgtc cccttttcgt tcacagcgcg
     3721 gagtcagtgc agggacgcgt ccccgggaac gacgcgccag ctcaggaagc ggacggtgaa
     3781 gtccggccgg gtaggggcgc agacgtaggg ccctgcggac acctcgccgg taacgaaggg
     3841 cgccacccgc accaaccgga agtcgccgcc ggcaaccttc gcgcggaccg tgagcgactc
     3901 cgcgtcccgg ctcacccgga cggtgatctc ctggccgttc caatcgaccg gtgcgaccga
     3961 ccagtccgaa cgctccagcg tcaccaccgc acccaggttg agcgcgccgt cggcgtactc
     4021 gcacccggcc ttcacccagt gttcggcgtc ggcgcgcacc agcacgccag cctggtcgaa
     4081 ctgccccgag aacgtcgtct gcagcaccac ctcgaccgcc tcgccgacgg cgagcggggc
     4141 cagcagcgcg tgctcggtgt cgtggacgaa tccgtaagcg gtgtgccgcc aggcgtccga
     4201 gccttccacc gcggtcactt caagcgcctt ctcggcggtt gtcacggcgg cgggcgggtt
     4261 ggtccagact ccttccgacc atgccacgcg gcgggtaccg tccggctcgc cccgaaccag
     4321 ccgcacaccg gccgcctcga actcggccag caccgagggc aggcgctccg ggctcaccgc
     4381 agcggtgtag tcgagcagca cggtggtagc gaagccgtcg cggacggcgt ccagcgcggt
     4441 cgcgcggacg cagtggtcgg tcgcgatgcc gcagacgacc accttggtca cgctgcggtc
     4501 caccagccag tcggcgagcg cgaccccgtc cgccctaccc tcgaaaccgg agtaggccgc
     4561 ggcgtattcg cccttgtcga agaccgcttc caaggtcgac ccgagatccc ggcctgcgcc
     4621 gggatgacgg cggtccggag ttgagccgag cagcgccgaa tgcagctcgg cgccgggtgt
     4681 cccggccacg cagtgcaccg gccaggagtc cacgtagtcg ggtgtcgcgg agaagtgcgg
     4741 tcccgggtcg atgtggtgat cgcgggtagc gacgacatgg tcgtaaccgt gctcgccagc
     4801 taacagagca ccgatccgag cagccaccgc agccccgccg gtcaccgcca gcgaaccacc
     4861 ttcgcagaag tcgttctgaa cgtcgacgac gatcagcgcg gtactcatcg cttaaccgcc
     4921 gcgtaagggt tgtcggtcac ctcgccgacc tcatcgagca ggatggtggg cagcgctggg
     4981 tcgcccttgc tcatcttgtg cgcggcgagc ggcagttcgg cccgcaccct ggtgtgccgc
     5041 tcccgggcgg cttcgagcgg ttcgcgagcg atcggcttgc ccttggccat cagctgcacc
     5101 agcagcggac ggtcgttgcc gtcgttgacc ggcggggtgc cgataccgat cacctccgcc
     5161 tccgcccggc ccgccgcgtt cagccggcgc agcgcgaact ttcgtccgcc caccgacccc
     5221 ttgttgacgc tcttcttcgc caccggcagc agttcggcgt ccttcgcttc ggacgtggct
     5281 ctcgacacca acttgtagac gaagccgcag gtcggcgccc ccgaaccggt gaccagcgag
     5341 gtgccgaccc cgtaaccgtc caccggggcg ctgcgtagcg cggcgatctg gaactcgtcc
     5401 aggtcagaag tgacgatgat cttcgtcttc tcggcgccca gggagtccag cagcttgcgc
     5461 acgtccgctg cctgctcggc gaggtcgccg gagtcgagcc ggatcgcgcc cagcttgcct
     5521 ttgctgaggc ggacgccggt gcgtacggcc tcttcgatgt cgtaggtgtc caccagcagc
     5581 gtggtgccct tgcccagcgt cttcagctgc gccttgaacg cgtcctcttc ggtgtcgtgc
     5641 aggagggtga acgagtgcgc ggccgtgccg gtggtcggaa ttccgtaggt gcgtccagct
     5701 tcgaggttcg aggtggcctg gaagcccgcg atgtaggcgg cgcgggcggc ggccaccgcg
     5761 gcagactcgt tggtgcgccg ggagcccatc tcgatgcagg gccggtcgcc ggccatcgcg
     5821 gtcatccgcg aggcggcgga cgcgaccgcg cagtcgtagt tgtagatgct cagcagcacg
     5881 gtctccagga tgaccgcttc ggcgaaggtg ccttcgacga tcagcagggg agagccgggg
     5941 aagtaggcct cgccgtccgg gtagccccag atgttgccct tgaacttgta gcggctgagc
     6001 cagtcggcca ggtcggtgtc gatgacgttc tgctcaagca ggaagttcag ctgctcctcg
     6061 tcgaagcgga aggcctggat cgcgtccatc gcgcggccgg caccggccac gattccgtag
     6121 cggcgcccct cgggcagtcg gcgggcgaag agttcgaaca cgctcctgcg aagtgcggtt
     6181 ttggaacgca atgcggcccg aaccatggtc agctcgtagt ggtcggtgag cagcgcagtg
     6241 ctgagctggc cggagcgcag cgtcgatggt gacacggacg acaggagagt catggcggtc
     6301 agcctagggc gtgtcgcaca actccgtgcg attgcgaggt gcaccgcagc gcgtgctggg
     6361 ttgtgcgacg cggcctagca tcccgacagg tccagaccga agggcatcgg ccatccgttg
     6421 ggttcagccg tggagaccct tcaccccgga acagtcgtgc gttacgcctc ggaaccagcg
     6481 ttgagcacgc ctgcagcgaa ctgatcctgg cgtacaggcg gcgggttccg ctgatcgggg
     6541 tgaaggcgtg tgcgcggtag aagctggacg cggcgtcatc gattgcgtcg acgaccagga
     6601 ggcggccgcc gcccgtggtg ctggccgcga ccacgagttc gaccgcgtgc agcagcaggt
     6661 acgtgcccag gccgcctccc tggaggctcc ggtcgagggc gaggcgagac aacaggtagg
     6721 cgggaatcac ggaatgcccg ccgtgcgcag ctcgggtcag gccctcgcgt cgcacctcgg
     6781 ttggggcgac cgaatagtag gcgaccaccg tggggtcgtc cgggcgagtc cagacgattg
     6841 tgcgcgcggt tccttgggcg tcggcgcgcg cggcgaactg tttgagccat gtgttcaggg
     6901 actccggtcc ggagtcgaac ccgtcgagaa ggtggctggg gctgatcctc gacgagaccc
     6961 agtgcgccgt caacggcggt acgggcggtc ggacgcggcc gcacgggcca gcttgtccag
     7021 cggcgctcgc tcggcgaggc tctcgacgag gacgtcgaac tggtgggcgg acatgatggt
     7081 gatgtcggcg cgagccaaca cccgctcagc cgactcgcgg acggcgatca ggatgaactc
     7141 cgaacgcgac tgcccaaccg ttctcgcggc ctcactgatg aggtcgtcca atgtgggctc
     7201 gagcctcgcc tccagccgac gtgtcttggt cccacctgcc atagaaggct tgcacggcag
     7261 tcgtacgaca ttctcatggc cggggtcaac aggagtgtcg gtcggcccgc ggctaccggg
     7321 cttgagcgtc ctgccgcaat tgggggctat gccagctttg tcggcgctgc ctgacacaat
     7381 ggttcggtgg caggcgagag cacgaccgag cggccgagcg caggggtggc ggtatccgag
     7441 cgtccggttg agtcggcgct gaccgggtgg gtgaccatcg tctgggacga tccggtcaac
     7501 ctgatgagct acgtcaccca cgtgttcacc acctacttcg gctatccgga gtcgaaggcg
     7561 accgacctga tgctgcaggt gcacaactcc ggcaaggccg tggtttcgac cggagcgcgc
     7621 gaacagatgg agaccgacgt ggacgcgatg cacggctacg ggctctgggc cacgctggaa
     7681 ccggtctgat gcgtgacttc cgccggcagg gcccggtgat caacatgcgc ctgaccgact
     7741 acgaggccac cctgctggag tcgctggtcg agcagttctg cggtctgctg gaagcggacg
     7801 ccgaggccgt cgaccatgcc gacccgttcg cgcggtggca ggctgagctc gacggcggtg
     7861 aaccactgga ccggaccgac ccggtgatcg tgcggctctt tccggacgcc tacccggagg
     7921 acccgatcgg ctccgacgaa tttcgccggc tgacgcaatc ccggcagcgg actgagcgcc
     7981 tcgaccaggc tgagatcgtg atgtcggctt tgcgggattc cgatgccggc aagcacccgg
     8041 tgcaagtgcg gttgatcgac ctgcccagct ggctgaagac cctcaccgcg gtgcgattga
     8101 gcctggccgt ccgattggga atccgcacca gcgaagatgc tgaggaattg gatgaactgc
     8161 ccgaaaccga tccgaaggca tacgtgtttc gggtgtacga gtgggtggcc tatttgaccg
     8221 agaatttgct cgctctcgcg tgatcgagcg ggcgcctacc gtttttggaa cgaggtgcgc
     8281 agctatttct cttaggctag ccttactaac atgtacgtcg gtatcgacct gggcagcgca
     8341 ccgctccaca ccagcctgac gctcgtcggc gcgccggacg gatcgcggca gcgcctctcc
     8401 tgcctgggcc tgcgggtggg gtcgacgttc tccctgctct cacggacggc cggcggcggc
     8461 cgggtcgcaa tggtgtccgg ctcccggatc gctctcggct ccagcttgat caaggagttg
     8521 cgggccgagg tcgtcgcctg atgccggtca ccgaaaccaa gccgaatcgg caactgaacc
     8581 tgctggtggc gagcgtgccg acgccctcgg tgaaggcgtg ctgccagacc ggcaccggta
     8641 cgggagccgg gccggacgcg ccagcggtgg ccctggtcgg cgccccgaac accggcaagt
     8701 cgacgctttt caacgctctc accggctcgc gcgtcacgat gggcaactgg ccgggcacca
     8761 ccgtcgaggt gtcccgtggc gtttggaaga ccaccaggga tgcggccacc tgcgactgtg
     8821 aagactgcac ctgcgacgag tccgaccaga aactcgacat caccctggtc gacctgcccg
     8881 gtgcgtactc tctcgacccg cactcgccgg acgaggcact gaccagggaa ctgctggtcg
     8941 gagtgccggc ggatgagcgc cccgacctgt gcatagtggt ctgcgacgcg gcccggctgg
     9001 cgaacagcct ctatctggtg tcgcagttgc gcgagcttgg cctgcggctc gtggtcgcat
     9061 tgacgatgct cgatgttgcg gccaagaacg gcgtcagcgt ggataccgca gccctttcga
     9121 aggcgctcgg ttgcccggtg gtggggatcg atccgcgtcg gcgcagcggc ctggagggcc
     9181 tcgcgggtac cgtccgcgaa acgctcggca cgcccgcgcc agccccgcgt ccgatggcgg
     9241 cggaactcga ctcgctcgaa ctggacgatg accgtttcag ctggatcgcg caggccgtgg
     9301 aggtcggcac caaggcgccg gccgatccgc agcacacgtg gtctgacaag gtcgaccgct
     9361 gggtgaccgc cccggtgctc ggcccggcga tcttcctcgg cgtgatgtgg ctggtgttcc
     9421 agatcaccac cgcggtcgcg gcgccgctgc aggacttcct ggacggcctg ttcactggcc
     9481 cgctgactga tggcgcgctg tggctgttcg cgcagttggg gctcaccggc acctggctcg
     9541 agggcctggt ggtgaacggg atcatcgccg gcgtcggcat gctgctcacc ttcgtgccgc
     9601 tgatgaccct gatgttcgtc ctgctggcgc tgcttgagga ctccggctac ctcgcacgcg
     9661 ctgccgtggt caccgatcgg ttgatgcgcg ccatcggcct gcctggacgc gccttcctgc
     9721 cattgatcgt cggtttcggc tgcaatgtgc cggcgatctc tgcgacccgg atcctgccca
     9781 gctcgcggca gcggattctg accgcgctgc tggtgccgtt cacgtcctgc actgcccggt
     9841 tgaccgtctt cgtgatgctc ggcagcgtgt tcttcggccc gtacgccggg accgcggtgt
     9901 tcgcgatgta cgtgatttcg atcctcctgg tcgttttcac cgggctgatc ctgcgccgga
     9961 cgctttggcg gacgatgggc gcggagcccc tgatcatcga cctgcccgcg taccagcgtc
    10021 cgaccctgcg cctgaccgct tcggtgacct gggtgcgact gaagggcttc ctgcagaccg
    10081 ccgccggcgt catcgtgatc acggtgtgcg cggtgtggct gctgcagtcg atccccaccc
    10141 agcccggtga gaggttcggg gacgtgccgg tggccgactc tgtctacggc gtcgcggccc
    10201 aagcggtggc gccggtcttc ggcccggcgg gcttcggctc gtgggagacc acctccacgc
    10261 tggtggtcgg gttcgtggcc aaggaggcgg tgatctcgtc ctgggcgcag acctacgcag
    10321 ttgaggagcc tgaggattcg gctgcccccg gcgacctcgg cgatgcggtg ctggcctcct
    10381 tcaccgaatc ctccggcggg catccggttc cggcggcgct tgcgttcatg atcttcctgc
    10441 tggcctacac gccctgcgtg gccactgtgg ctgcgcagtg gcgcgagatc ggtggccgct
    10501 ggacgctgtt cggcatcggg ctgcagttgg cgatcgcctg ggggctcgcc gtcgtggtgt
    10561 tccaggtcgg gagcctgttc ctatgaccgc cgcggctggg gtgcggcgcg gcccgttgag
    10621 cctggtgctc gatgagatgg cggtcggaac gcccacggtc gcgcagatgg cccggaatct
    10681 cgcgctcgac gagcaggtgg tgcgggctgc ggtggaccac ctggttcgtt cgggacgggt
    10741 atccgcgcgc gaactctcga tgggctgccc ggcctcgggt tgcggcggct gcgcgtccgc
    10801 ggtggacggc gagcccggct gcggccttcc tgctcccgtg cccggccggc gcgccggcct
    10861 ggtgacgctg accctcaccc gccgctgaac cgcggttgac cgcgtccgcg tccggccccg
    10921 aagttgccgg cgaaccgggg cacgaggcgg aaggcgtcac gaagggcggc cgctaccttt
    10981 gtggtctggt ttggcgaggg gccgcctcac agcctcacca gatctccggc ggttcccgtc
    11041 gatcggccgg cgccgcggcc ggcttctctt cgcgtccggt gaacgcgcgc caggactcgt
    11101 ccagcttgcg ccggccgtcg tccaaccagc ggagggtgcg cagcacgatc cgggcggcgt
    11161 cgatctgatc ggccaagtcc catccctccg gctgcggttg gcacccattc tgccgcgggc
    11221 ctaaagtcgg tgctcgtgat cgcgtccgac gccccgatcg gggtcttcga ctccggcttc
    11281 ggcgggttga ccgtagctcg tgcgctgctc gaccagctgc ccggtgagga catggtctat
    11341 ctcggtgaca cggcccgcgc gccgtacggc accaagtcga ttcccgaggt gcgcgaatac
    11401 gctctggaat gcctggatta ccttgccgag tccggagtca aagccctggt catcgcctgc
    11461 aactcagcct cgtccgcggt gttgcgggac gcacgcgagc gctacgacgt acccgtcatc
    11521 gacgtgatcc tgccggccgc gcgccgcgcc gtccgcgcca gcaagagcgg acgcatcggg
    11581 ctgatctgca ccgaagccac ggcgacttcg gcgagctatg acgacgcgct cgcagccgtg
    11641 cccggtgtac agctgcttac cgctccgtgc ccgctgttcg tggagtacgt cgaagccggc
    11701 atcaccggcg gccccgagtt gcttgacgcc gcccgcggct atttgttgcc gttgcgggag
    11761 gccggcatcg acacgctgat cctcggctgc acgcactacc cgctgctggc cggcgtgatc
    11821 tcctacgtga tcggcgacga ggtgaccctg gtctcgtccg ccgaggagtg tgccaaagag
    11881 gtgttcgcca cccttaccga caccggcctg gtgcgcagcg ctccgcggac gccgaccctg
    11941 cgttttctga ccaccggcag cccggaactc ttcgccggca tcggccgacg gctgatgggc
    12001 ggtttcgtcg atgaggtgga gcaggtctac acgatcgcgt gacgcgtccg gacccgcctt
    12061 cgcgtgtcgt gatctcgaag ctggatgctc ggcagcgaac gcatctggcc tgatcggggc
    12121 atcgcgctgc gagatcgcgc catcgcgcgg tggcatgacg cgattccgac cgacgatcgt
    12181 ccgggacgga accctcgcgt cgtttacggg tgtcgtgggt gcggatcgcg tcatctcgcg
    12241 cgaagggccg ggcaaggcga tcatctgcga gatctaccgc tgggttggag aatcgcggcg
    12301 gggcgtgtcg tgatctcgga ggtggatgct cggcagcgaa cgcatctggc ctgatcgggg
    12361 catcgcgctg cgagatcgcg ccatcgcgcg gtggcatgac gcgattccga ccggcaatcg
    12421 tccgggacag ggctcttgcg tcgtttgcgg ggcgtcccgg gaccagattt tccgtcagcc
    12481 tcgggtgagc tagccggtga cgacctcgcc gccagccgac ttccaggcgt tgatgccgcc
    12541 ggccaggtcg tagaggttgg tgaagccgac gccctgcatg gcggtcatcg cgaccttgga
    12601 gcggttcgcg ctgcggcagt aaaccgcgta cggcttactg gggtcgaggc tggcgatgcc
    12661 ggccgcgaag tcgggcgact ccacatcgat gttgaccgct cccggcaggt gtccggctgc
    12721 gaactcctcc gcggtgcgga cgtcgagcag cacggtgccc ggcagcctcg cggccgcggc
    12781 gaaggcgctg gcatccagtg aggagcccgc ggccggtgcg gcagtggctg tcgggccggc
    12841 agagacgctc accgaggtgc tcgagcagcc gctgagcagg gcagccgatg cgaggagcgt
    12901 gaccgccgct gcagccaaac gtccggtgaa tcggcgagag ctcatcggcc gaagatcttg
    12961 aacagtccgc tggacttctc cggcgcggtc gcggccgcgt gtccaccgca ccactggttg
    13021 gccgggacca tggccttgac ctgagcgacg tgctggccgc agccagccca ggtggtcttg
    13081 ccacagacct tgcaggtaac cggacgacac atctcgcaat ctccattctc gaacgatgaa
    13141 ccaagcatac cccagggggt atcagtctga acaaatcggc gtcccgcacg gcccgctcag
    13201 gcgttgtcgg agccgcccga tagggttggc gcatgtcaac accaagagcc gacggccgat
    13261 ctccactgga cctccgcgag gtgcggatta cccgcaactg gctcgaccat gccgaaggat
    13321 cggtgctcgt cgagttcggt gctaccaggg tactgatcgc ggcttcggtg accgaaggcg
    13381 tgccgcgctg gcgcaagggc agcgggctcg gctgggtgac cagcgagtac gcgatgctgc
    13441 cgcgggccac gcattcgcgg agcgatcgcg agtcggtcaa gggccgcatc ggcggacgca
    13501 cccacgagat ctcccggttg atcggccgca gcctgcgctc ggtgatcgac tatcgcgcgc
    13561 tcggcgagaa caccatcgtg ctcgactgcg acgtgctgca ggccgacggg ggcacgcgga
    13621 cggcgtccat caccggggcg tacgtggcgc tcgtggacgc ggtgacctgg ttgcgaaacc
    13681 gcggcgcgct cgctggcgaa ccgctcaccg gcagcgtggc tgcggtatcg gtcggagtcg
    13741 tcgacggcac ccccatgctc gacctctgct atcccgagga ctcctccgcc gaggtcgacc
    13801 tgaacgtggt cgcaaccggc gggggcgagt tcgtcgaggt gcagggcacc gcagagggca
    13861 agccgttcgc gcgtccggta ctggacgagt tgctcgatct gggtctggcg ggctgtgcgg
    13921 aactcacccg gctgcagcag gcggcgttgg ccagatgagc atcgtcgtcg tcgccaccca
    13981 caacgcgaag aaggccgccg agttgcaccg cgtgctcgct gcggcggggg tggacgcgga
    14041 tgtgcgcggc ctggatgcct tctccgccta tcccgagccg gccgagaccg agcggacctt
    14101 cgagggcaac gcgctgctca aagcgcgggc tgcggcggcg gccaccggtg agctggcggt
    14161 ggccgacgac tcgggcatcg aggtcgatct gctgaacggg atgcccggag tccgctcggc
    14221 acgctggtca gggccggacg cgaccgacca gcgcaacctc gacctgctga tcgcgcaact
    14281 caccgacacg acagacgccg accgcaccgg acgcttcgtg tgcgccatgg cgctggtgac
    14341 gccggagggc gccgaacacc tggtgcgggg ggtgatggag ggacggcttg tcctcgaacc
    14401 gcgcggcacg aacggcttcg gttacgaccc gatcttcatc gctgagggta acgaggtcac
    14461 caacgccgag ttgacccccg agcagaagga cgcgatcagc catcgcggcc aagcggtgcg
    14521 cgcgatggtc ccgatcctga gcgaccttct aggccgatcg aagtcgtcga cccaggacgg
    14581 agcggtccag tcatcgtcat ctcagccctc accttcctca tcccagccct caccttcgtc
    14641 atcccggcgt aggccgggat ccccggatcc tggacctgag ccgaaggaaa gctgatgcag
    14701 caatacctcg acctactgca acacgtgctg gatcacggca cggcgaagtc tgaccgcacc
    14761 ggcaccggca cgcggagcgt gttcggctac cagatgcgct tcgacctagc cgttggcttt
    14821 ccgttgctga ccaccaagag gctgcacacc cgcagcatct tcggcgagct gttgtggttc
    14881 ctgcggggct cgacaaacgt cgagtggctg cacgataacg gcatcacgat ctgggacgag
    14941 tgggccgacg cgaacggcga cctcgggccg atctacggct accagtggcg gtcctggccc
    15001 accccggacg gcggtcatgt cgaccagatc aagggcgtga tcgagtcgat ccagaacttc
    15061 ccggacagcc gtcggcacat cgtttcggcc tggaacgtcg gacagttggg ggagatggcg
    15121 ctgccgccgt gccacgcgtt cttccagttc tacgtggcgg acgggaagct gtcctgtcag
    15181 ctctaccagc gctcggccga catcttcctc ggggtgccgt tcaacatcgc gtcctatgcg
    15241 ctgctgaccc atctggtcgc gcaggtgacc gggctggaag tcggcgactt cgtgcacacc
    15301 ctcggcgatg cgcacctgta ctcgaaccac ttggagcagg ccaagctcca gctgacccgc
    15361 gagccgcgtc cgctgccgag gctgctggtc gacccgagcg tgaccgagat cgacgccttc
    15421 accctggccg acctcgaggt ggtcgactat gacccgcacc ccggaatcaa agccccgatc
    15481 gcggtctagc catgcggatc atcgccatcg caatcgtggg cgccaacgga gtgattgggg
    15541 acggggagcg ccagccgttc gagatcgctg aggactgggc ccgattcaag cgggtgacgc
    15601 ttggacatcc gctgatcatg ggacgaaaga cgcacgatgc gatcggccgc tggctgccca
    15661 agcgcaccac gatcgtggtc acgtcgcacc ccgagacgat cgcgctccct gagcccactc
    15721 gggtcgtcga gcctatcgaa accctgccgc cccgtaccgc ctacgcggtg gcctccctgg
    15781 acgaggcgct cgacctggcg gccggcctcg acgccgagat ctacgtcgcc ggcggcggca
    15841 gcatctaccg gcaggcttgg ccgcggctga ccgacctcga cctgaccgag gtgcatcagg
    15901 ttgcggacgg ttcagtgctc tttcccgacg tgcgcccggc cgagtgggtg gaggtgtccc
    15961 gcgagccccg cgacggtttc gactttgtcc actaccggcg gcgctgagcg atgttgctcc
    16021 cggccaggat cgctgccggc gtgcgcgacg gcaccatcac tttggcgttc cgccggtggg
    16081 agcaaccccg cgtccgggtc ggcggcaccc agctgaccag tgccggcatc gtcggcttcg
    16141 atgccgtcga ggaggtcttc ggtgcagagc ggctcaccga tgcggacgcc cgcgcggccg
    16201 gcttcgcgag cctgaccgcc ctgcgtcgcc agctctaccc gcccgaaccg ccccctgagg
    16261 aggttgctcc gcgagagccg cgttgggtcg gcccccgcgc cagcaaaggt ggctaccacg
    16321 tgtaccgggt ccgtctctct tgggtgggcg acgatccacg tgtcgcgctc cgcgacgccc
    16381 tcccgagcgc cgaagagctg accgaaatca ccgcagccgt cgccaagctt gacgcagcca
    16441 agcgcaccgg gggttggacg cgtccgatcc tcgagtggat tcgcgaccac cctggggtga
    16501 tttccaccga actcgcagcg ctgctgcaac gggaactgca gccgatgaag accgatatcc
    16561 gcaagctcaa ggcgctcggg ctcaccgaga gcctccgggt cggctaccgg ctatcaccgc
    16621 ggggtcgggc ctatctcgaa tcgctggact gaccttgcgt ccgcaagctt cgatcatgca
    16681 cctcgatctg gacgcgttct tcgcggcggt cgagcaacgc gacaaaccgt cgttgcgcgg
    16741 caagccggtg atcgtcggcg gcatcggact gcgcggcgtg gtctcgacag cgtcctacga
    16801 ggcacggaag ttcggggtgg gctcggcgat ggcgatgggg gaggcgcggc ggcgctgccc
    16861 gcacgccgcc tttctggccg ggcggttcgg ctcctaccgc gaagccagcg cgatcgtaat
    16921 gcggctgctg catgagcttt cgccgctggt cgagccgctg agcctggacg aggctttcgt
    16981 cgacctggcg gccggccccg agcgcgactt ggatgacgac tccctgttgg ccctcgcgca
    17041 ggacctgcgc gccaggctgg cggacgcgac gggcgggctc actgcttcgg tcggtctggg
    17101 cagttcgaag ttcatcgcga aggtggccag cgaactggcg aagccgaacg gcgtccggct
    17161 ggtcgccccg gggactgagg ttgccacgat cgccgaactg ccggcccggg caatcccggg
    17221 cgtcggtccg gcgacgatgg accgattgtc ccgattgggt gtaagcacgg tggccgacct
    17281 ccaacggctc agccgcgccg agttggtacg cgagctcggc aagtccggcg gcgagcacct
    17341 gtccgatctc gcctgggcgg tggatgaccg accggtcgaa ccggaacgcg agacgaagtc
    17401 gatctcggtt gaggacacct tcgagcgcga cctggcggac gcggatgtcc tggccggcat
    17461 cgccgaccgc caagccattt cggtagttca acgtctgacg aaggccgagt tgttcgcgcg
    17521 gacggtgacc gtgaaggtgc gttggccgga cttctcttcg atcagtcggt cccggacgct
    17581 caacggcgcg accgaccggg tggaagtggt cgcgcaggtg gcccgcagcc tgctggaggg
    17641 gctggacacc agtcagggtg tccgcctgct cggcgtgggc gtctccggtc tggtcgaggt
    17701 ggcgcaggag ccattgttcg acctggtgga cagcgctcca ccgcgggtcg tcgaggaggc
    17761 cgaggccccg ctgaccaggc gtccgtccgg ttggctggcc ggagcggacg tggtgcacgt
    17821 ccagttcggc cagggctggg tatggggctc cgggctcggc cgggtcaccg tccgcttcga
    17881 gaccgcagac accggtcccg gcccggtgcg cacgttcgcg gccgacgacc cggaactgag
    17941 ccccgcctga tcgcgctcgc gtcgtacccg cgtgtcgcct gatcgcactc gaatcggacc
    18001 cgtgtcccgc ttaccccgct caaccgccga gggctggcct tggcggttgg gacgcccacc
    18061 tcaggttcga gcccaaggct ctaagcctgg attcaccgat ggcagtcgtc gcgagtggca
    18121 ccgggcgggt gccctggccg gccgagccaa gcgatggcat gccgggtccg tgtagttgag
    18181 cgtggtcggc tgcgccaggg cgcctgtata gggttgcgca gcagaccgaa ccggtgatcc
    18241 ggggctgcct gggcctgacc aatgctcatg tccacaactc agtcggcggc cgcgctcacc
    18301 tgctgcgggt tcaccggcac cgattcgcgg ctaaccccca tcgaaaccgg gtttcacctg
    18361 caacagtcgg acagacccgc accagatgcc atcaaagtcg ggctcaactg cccgacgcca
    18421 accaggacac cggtctggcg ccgcccgtga tgacggccag cggtgcatcc gggccaagct
    18481 aaggctgacc ctcggtgagc ggacgggcgt ggtcagctgg ggcagacgcg cccgctagac
    18541 tcaagccgcc agccggagtg gtggaattgg cagacacgca ggatttaggt tcctgtgcct
    18601 tcgggcgtga gggttcaagt cccttctccg gtaccgcgcg actcgtgttg tcgcacgatg
    18661 aaagcactcc attcaggccg agagcgggct ccaggaatcc ggcaacaggt cgaccgctgg
    18721 cggagcagat gtcacggcca ccggctcccc ggacgcgatc gactcctcta cggccagcat
    18781 gatgtcgagc acgtggtagg ccagttctcc attcagccgc ggacgccgcc cctcggccag
    18841 ggaccgcacg aggtcgacga ctccgatgcc gcgaccgtgt ccggcggctt cgatcgccac
    18901 ctcgccggtg agttcgccac cgacatgggt gcgtaccgtt ccgtcgaaca tgttggggtc
    18961 cggcgccacg atcgtgccgg tgctgccttg gaactccaac accccggcgc gcgcgagccc
    19021 cgagtcgaac gagtacaggc acaccgacga agccccggac gcgtgccgca gcagcacatt
    19081 ggcggtggtt ggcacagtca cggcgaactc cgtcccagcc ttcggcccgg acacgaccgt
    19141 ccgcacctcc cgagcccgac cgcccgcggc gaccacggaa gtaaccggcc caagtcccag
    19201 caccagtgag gtgaagtagt acggcccgat gtccagcacc ggccccgcgc ccgtctggaa
    19261 caggaaggcg gggtccggat gccaaaggtc cgggccgggc acctgcatga tcgccaggcc
    19321 agcgagcgga cgcccgatcg agccggcgtc catcgaacgc aatgccgcct ggatgccagg
    19381 accgagcacc gtgtcaggtg cgcaacagac ctggacgccc agcgcggcgg cctcatcgag
    19441 caacgtgcgc gccgccgtcc ggtccagacc gagcggcttc tcgctccaca cgtgcttgcc
    19501 ggcccggatc gcctgggccg agaccgacag atgcacctcg ggtggcgtga tgttgaccac
    19561 cacctcgacc tcagggttgg cgcaaaccgt gcccgcgccg ccccacgtat cgacaccgaa
    19621 cttctccgct tgtgcccggg cgcgctcggg aatcaggtcg tcgatcgcga ccactttcag
    19681 gtcgggatag gtggtgaggt tggtcaggta ctgcgtcgag atgttcccgg caccaaccag
    19741 gcccacgccg atcggcatca ccgctcccgg aggtagccga tgctctggcc gacctcttcg
    19801 agtgggctcg tcgccagatc gaattcgacc acccagaccc ggtcgggcga tgcgtcgagg
    19861 atgggcggca ggtccatcga cccggtgccg aggacagcgt tcagcgcgcc actgtctgac
    19921 ggcagcaagg gcccgtcctt cacatgcagg tgggtgatcc ggctgccgta cttcgccagg
    19981 agggcgggca cgtcgcgtcc gccggccgcg gcccagtagg tgtccacctc gagcacgacg
    20041 cgctcgtcca gcagggacac gaagtagtca taggccggga cgccgtccac gtcgggcgcc
    20101 gtgaactcga agtcgtggtt gtggtaaccg atggtcacac cggcctcggc gaaacgatcg
    20161 acctggctgt tcagcgcttc ggccaatccg cggatcccgt ccgcggacgt ccactgcacc
    20221 ggctcccaat acggctggaa caccgtgcgg acgccgagcg ccgtcgccgc tgcgagcacc
    20281 ttctccggat cgtccagcag ccccgcgtgg gcgcttccgg ctttcaggcc gtgcgcggca
    20341 agcgaatcgg cgagccagtc gaagtgttct atgccgaatg gctcgacctc gtccacaccc
    20401 atcccggcga cggccgcgag ggttgtctcc gggtcggacg cgagctggtc tcgaagtgtg
    20461 tagagctgga tgctgacgcg ggccacagtg cctcctggat ggaaatgatc gcgctctcac
    20521 agtatcgcgg aaccgtccct ccgcaacgtc ccgcgagaat cgattcgtcg gtgcgggcca
    20581 ggtcggggcg agtcagccga gcacggtacc ggtgtgatcg gcgagcgcgg tcagcaggga
    20641 gtcctggaag gtggggtcag ccgtactcgg gtgtggcgga cgctggcgtt ggtggtgcca
    20701 gtagccacca gagaccagcg caggcgccgc ttcgctggtc gcaagccatt cctgggtcag
    20761 gtgcccgagt cgcaggtcgt ccggcgcgcc cggaccaccc atcctggtcg gcacccagcc
    20821 ggggtcgacc gcattgctca acacaccggg ccagaaccgg gcgaccgcaa gggccagcgc
    20881 ggtgacgaac agcttgctgt cggaatacgt tgcggtcacg ctggcgccgc cccagtcgag
    20941 accggacaag tcgggacggc cgccacggtg caggccgctg cttaggtaga tcagccgtcc
    21001 cggacgatcg atgcgagcgg tgagtaggta gggtgcgacc acgttcaccg gcaacaccgc
    21061 cgggcccgag accacgcccg cgttgtgaat gaccgcgtcc atccggccaa ggcggttcac
    21121 ctgctcggcg acgtccatgg tcgcggtgag gtcgctgaga tcgccgacga cagccttcgc
    21181 tccctgcgcg attaggccct gcacggcggt gattcgtgac tggttgcgca cgtgaaccac
    21241 gacttcgtgg cccacggcca gcagggtggc ggctgccgca tgcccgagac catcgcttga
    21301 cccggtgatg aagacgcgcg ccatgtgccc ccctcggtca gaggccggta gccagggcct
    21361 gtgcgatcag tcggctaccc agctcgccgt aggcctccgg atcacggttg accgacatgg
    21421 cgcgaagtgc ggcgtagccg gccacgaggc ccatgatcag gtcgaccaga tcgtccgggt
    21481 cggtgtccga cgaatgctcg gcacaccagt tcgccatgaa ggtgcgaacg gcgctccggg
    21541 ccaccatcgc ttgggaatgg atcaggcggc ccagcttcgc gtccgacttc gccccagccc
    21601 agatctgcag gagcagttca ggggtggttt cgtcgcgcgc catctgcgcg agggcggccc
    21661 ggaagagctc cgtcggcgac agttccggac tggtccgaag caccatttcc atcggcttgg
    21721 ccaggatgct ctgaccgacc tcgaggatca accgctcctt gttgccgaag tggtggtaga
    21781 tggcccccgc ggacgtcttc gagcgggcga cgatgtcgga gatcgaagtg ccggcgacac
    21841 cctggtgccg gatcagatcg atagtggccc ggataattgc ctcgcgggcg tcgcgcgcgg
    21901 gcgcgggcac gttctccaca atctgcaggg tactgccgcg ccgctcacga ttcggccatg
    21961 gccagaatgc agaacgatcg ttctgctagg ctcgcccacg agcagaacga tcgttctgat
    22021 tggagggccg atgccccagc agttcgcgct cggactcgat atcgggtcga ccacggtcaa
    22081 ggccgtcgtg ctcaacgccg gtacggttcg gttcagcgcc taccgtcgcc acaacgcgga
    22141 cgcgcgcggc gcactgcgga ccctcctggc tgacgtcgcg cagcgatacc cagggcagca
    22201 gttccgctgc gcggtgaccg gctccgctgg tctgggggtg gccgacctgt tgggcgtcga
    22261 gttcgtgcag gaggtcatcg ccagcaccgc ggccatcgag cgcttcattc cggacgccga
    22321 tgtggtaatc gaactcggcg gtgaggacgc gaagatcacc tttctgcggc cggtggtcga
    22381 gcagcggatg aacggcacct gcgccggcgg caccggtgcc ttcatcgacc agatggccac
    22441 gctcctgcac accgatgccg gcggactgga cgacctcgca gccaagcaca agcacctcta
    22501 cccgatcgcc tcccgctgcg gcgtgttcgc caagtccgat ctgcagccgc tgctgaacca
    22561 gggcgcggcc cacaccgacc tggccgcatc cgtgttcgcc gcggtcgcca cccagacgat
    22621 cgccggcctg gcctgcggtc atccgattcg cggcaacttg gtcttcctcg gtggaccgtt
    22681 gcacttcctg ccgcaactcc ggcgtgcgta cgagcgcgcg ctcgcaactc aggtcgactc
    22741 gttcaccacg ccggacgagg cgcacctcta cgtcgccatc ggggcggcgc tgctgaccgg
    22801 ctcgggctcc ggtgtggcca tcgaggacct gatcggacgg cttcgcgaac gcaccctgat
    22861 cccactcgcc agcacgcgga tgcgtccgct gttcaaggac caggccgaac gcgacgagtt
    22921 cgacgtccgg cacggcagcg agacgatcgc gagaggcgac ctcagcgcgg cgtccgggcc
    22981 gatgttcctc ggcatcgacg cagggtcaac gacggtcaag gcggtggtgc tgaccgcgtc
    23041 gggtgaactc gtgttctcgc actaccaggg cagcaacgac cctgtcgggg tggcgctcag
    23101 catcctgcgg accgtgctca cgtcgcttcc agcggacgcc tacctggctc gcgcctgcgt
    23161 gaccggctac ggcgaaggcc tggtgcgggc cgcgctgcgc ctcgacgacg gtgagatcga
    23221 gacgatggcc cactaccggg ctgcggagcg gatcgcgccc ggggtgactt ccgtgatcga
    23281 catcggcggc caggacatga agtacctgcg catccgcaac ggtgctgtcg actccatcgc
    23341 cgtcaacgaa gcctgttcgt ccggctgcgg ctcgttcctg cagacgttcg ccgcgtccat
    23401 ggacaccgat gtcgccgcct tcgccagcat ggcgctggag tcatccgcgc cggtcgacct
    23461 gggcacccgc tgcacggtct tcatgaactc gtcggtcaag caggcgcagc gtgagggtgc
    23521 ggcggtcggc gacatcgctg ccgggctctc ctactcggtg gtgcgcaacg cgctgtacaa
    23581 ggtgatcaag ctcaaggacg cgtcccagct cggcgagttg gtggtggtgc agggcggtac
    23641 cttcctgaac gacgcggtgc tgcgggcctt cgaactgcag accggcgccc aggtgatccg
    23701 tccggacatc gccggcctga tgggcgccta cggtgcggct ctgaccgccc agcgcaattg
    23761 gtcggacggc gaacgctcgc aggtgctcgg cctcgatgaa ctggacgagt tccgcgtgga
    23821 cacgcatctg gacacctgcc ggctctgcca gaaccactgc cagctgacca tttccacctt
    23881 cggcgatggc acccggcatg tctctggcaa ccgctgcgag cgcggtgcga gcactgagaa
    23941 ggtgccgaag aagtccgagt tgccgaacct ctatgactac aagtaccggc gcatgttcgg
    24001 ctaccggcga ctgactgctg acttggcatt tcgcggcgat atcgggattc cgcgggtgct
    24061 gaacatgtac gagaactacc cgctgtggtt caccatcctg acccggctcg gcttccgggt
    24121 gaccctttcg gggcggtcca gccacgaggt gttcgagcgc gggatggagt cgattccggc
    24181 cgagaacgcc tgctacccgg ccaagatcgc gcacggccac atcgagagcc tgctcgaccg
    24241 gggcatcaac acgatcttct acccgtgcgt cccgctcgag gccaaggagg tcaagggggc
    24301 ggacaaccat ttcaactgcc cagtggtcgc gttctacccg caggtgctgc agcgcaatct
    24361 ggtgcggctg caggagccgg gcgtccgcta cctcagcccg ttcctgaacc tggacaaccc
    24421 cgagaagctg gccaagcggc tggtcgaggt gttcgccgac tgggacgtca ccctggccga
    24481 ggccacggct gcggtcgagg cggggttcat cgaggacgcg aacgccaagg ccgacattcg
    24541 ggccgaggga cgccgtgcac tggagtacat gaccgcccac ggcgtcaagg gcatcgtgct
    24601 ggccggccgg ccgtaccacg tcgacccgga gatcaaccat ggcatcccca gcctgatcaa
    24661 cggcttgggc atggccgtac tgaccgagga cgcggtcgtc gaaccgtccg cgacggccct
    24721 ggcccggccg atccgcgtgc gcgaccaatg ggcctaccac acccggctgt acgagtcggc
    24781 ggcggtggtc gcgaagcagc ccgacctgca cctggtgcag ctcaactcct tcggctgcgg
    24841 ggtggacgcg atcaccaccg accaggtgca ggagatcctg gagcaggccg gcgaggccta
    24901 cacgctgctc aagatcgatg aggtgtcgaa cctcggcgcg gcgacgatcc ggctgcggtc
    24961 gatgaaggcg gcggcggccg agcgccgcgg ctcggccacg gtgagcacgt tcggaccgct
    25021 gggggagcgg cggctgttcg acgagaacgc ccgcgacacc catacgatct acgccccgca
    25081 gatggcgccg atgcacttcc ggctgctgat gccggtgttc ggcaaggtcg gactgaacat
    25141 gaagttgctg gagcgggcca gcagcgacga cgtggaagtc gggctcaagt atgtgaacaa
    25201 cgacgcctgc ttcccagcga tcatggtggt cggccaactg gtgaacaaga tccgcagcgg
    25261 cgaggctgac ccggaccgca cctcggtggc gatcacccag accggcggga tgtgtcgtgc
    25321 aacgaactac gttggactac tacgcaaggc ccttgccgac gcgggctacc cgcaggtgcc
    25381 ggtgctggcc atcagcgtgc agggcctgga ggcgaatccc ggcttcaaga tcacgccggg
    25441 actgatccat ccggcactac aggcgctggt gctgggtgac ctgatgcaga cggtgctgct
    25501 tcgcgtccgg ccctatgaag tggtgccggg ttcggccaag gcgctctatg accgctggga
    25561 ccggatctgc caggagttcc tgcagcactc gggctactcg gcgacgctgg ggcgcagcgt
    25621 tgggtacacc gcgttgattc gtaggctggt gcgggagttc gacgaactgc cgctgcgtga
    25681 ggttccgcgc aagccgcgcg tcggcgtggt gggggagatc ctggtgaagt tccacccgga
    25741 cgcgaacaac cacgtggtgg atgtgatcga ggccgaggac tgcgaggcgg tcattcccgg
    25801 cattctgcag ttcttcctga tgccgctgta ctcggcgcag taccagtggg agaccctcgg
    25861 catcggtcgc aagtcgcacc acgtgaagaa gctggcgacg tggctgatcg agaagtacca
    25921 gcagccggtg gcgcaggcgt tggcgaagac cggcaagttc gacgtgccgc cgcgaatgag
    25981 cgatctggcc aagaaggcgg acggggtgat ctcgctcggc acccgggcgg gcgagggctg
    26041 gctgctgacc gcggagatgg tcgagctgat cgaaatcggc gcgccaaaca tcatctgcgc
    26101 ccagccgttc gcctgcctgc ctaaccatgt ggtcgggaag gggatgttcg ccgaactgcg
    26161 gcgactgcac ccggaggcga acatcgtcgc ggtcgactac gacccgggcg cctcggagac
    26221 caaccagctc aaccggatca agctcctggt cgccaccgcg cacaagcggc atcacgccga
    26281 gcagcacatc gggcgcgctg attcggtcgg gcttggggag ccgcagctcg gtacggcgcc
    26341 ggtctcgccc gcggacgtct gggatctggt ctgactattc gttgggttcg ctcgtcggcc
    26401 tggtgaagaa ggccgggttg tcggcgacgg ggcgcggacg cccgtcctcg tcgacggcca
    26461 cgtagcggaa gacgccctcg gcgacgagtt cgcgttcctc ctcatagatc gcgaccaggc
    26521 cgcgggccca gacctcgagc ttgaggtcca gcgagctgcg tccgaccttg agcagttcgg
    26581 cgtggacgca gatcgtgtcg ccgaccgcga ccggctggtg gaacaccaca tcggtgacgg
    26641 tcacggtgac cactcgtccc tgggcgattt cggcggccat gatgccggcc gctatgtcca
    26701 tctgcgagag gatccagccg ccgaagatgt cgccgcgagg gttggtgtcc ctgggcatgg
    26761 ccaaggtgcg cagcaccagc ggaccgcgcg gatggcgccg ctcaacgtct tgatccatgt
    26821 tggtcccttg ccattgccgg tttgaactca ggctatgggt agcggggtgg cgtcgccacc
    26881 tggatggatg tgaacagtgg gaggttttcg ccgcgcggtc cggagccaca tgggatcatc
    26941 gaagttccat gcacgggatc gcacagggga gtgagatgac cgacaccggc aacgaggtgg
    27001 acctgagcta tcgcgacgcg agtctgccgg tcgctgaaag ggtggagatc ctgctcggcc
    27061 agatgacgct gccggagaag gccggtctgt tcttccagac catgatcacc atgggcgcgg
    27121 atggctcgct caccgacggc gacccggtct tcggactgcc gagcacggac gagtacgtca
    27181 acggccggct gatgaaccac ttcaacatcc tcactgcggc gccgacgccc gaggcgatgg
    27241 ccacctggca caaccggctg caggaactcg ccgcgtccac ccgtctcggc attccggtga
    27301 cggtctccac cgacccacgg cactcttact ccgacaaccc gctcgccgga ctgagcgcgg
    27361 gctgcttctc ggtctggccg gagccgctcg ggctcgccgc gatcggcgac gaggcggtgg
    27421 ttgagcgctt cggcgacatc gcgcgccagg agtacaccgc ggtcggcatc cgggtggcgc
    27481 tgcatccgca gatcgacctg gccaccgagc cgcgctggtc gcggcagctg cagaccttcg
    27541 gtgaggacgt cgaactgact tctcgcctgg gcgcggcgta catccggggc ttccagggac
    27601 gcgtgctcgg caccgactcg gtggccacca tgaccaagca cttcccgggc ggcggccccc
    27661 agctcgacgg cgaggatccg cacttcgccc acggccgcga acaggtctac ccaggtggcc
    27721 agttcgaact gcatctcaag cccttcgagg cggccctcga ggccggcacc agccagatga
    27781 tgccgtacta cgggatgccg atcggcaccg agctcgagga ggtcggcttc ggcttcaaca
    27841 aggcggtcgt caccggcatg ctgcgcgagc gctacggctt cgacggcatc gtctgcaccg
    27901 actggggcct gatcaacgac gccgcgatca tgggtgcacc gttcccggcc cgcgcctggg
    27961 gtgtcgagca cctcagcccg cgtgagcgca tggtgaaggt gctggcggcc ggctgcgacc
    28021 agttcggtgg cgaagcctgc ccggagctgt tgatcgagct ggttgagtcc ggcgagatca
    28081 gtgaggagcg gctggacgtg tccgcgcgcc gactgcttcg ggagaagttc gtcctcggac
    28141 tgttcgaccg gccgttcgtg gaggtcgcga gggcgtccgc ggtggtcgga tcgcccgagt
    28201 tcgtcgccgc aggattggcc gcacagcgtg cgtccctgac cctactcact aaccgtgcgt
    28261 acggtgccgg tgaatcggtg ctgccgctcg gcaccgggcg aaagctgtac gtcgaaggca
    28321 tcgactcggc ggtcgcaggc cgctacggga cggtcgtggg cgagccgggg gaggcggatg
    28381 tcgcgatcct ccggctgcag gcgccgtggg agcagcgtcc gacgatgttc gagaacttct
    28441 tccactccgg atcgctcgac tttgccgacg atgtggtcgc gcatgtgcgc gaggtagcgg
    28501 cagtggtgcc gacggtgctg gacgtgttcc ttgaccgtcc ggcgatcctg accccgttcg
    28561 tggacgtggt tacggccatc gtcgccaact ggggcgccag tccgaccgcg ctgctggacg
    28621 tgctgaccgg cgtcgaccag ccgcagggct cgctgccctt cgacctgccg agcagcatgg
    28681 ccgccgtcga agcgaacctg ccggacgtgc ccttcgacac cgccgagccg ctgttccggt
    28741 tcggccacgg tctgcgctac gcgtccgccg gctgaggctg cgctcggaca aaggggccgg
    28801 cggaacccgt aggatcgatg tcgcgatctg ggcaatagat ggtgggggtc gtcggcaccg
    28861 acgcttctag gacgtcaccg cttcgagatc gcgacacgcg gctggtcggt ggcgccagtc
    28921 gggatcccct gcggccggct cgtcgaacga ctgtcagtcg acggcggtta ccaggtagag
    28981 cagcgcgtgg tcggggttga ccgcggcatc ggtcaggccg gtgtgcagca gcaccgagcc
    29041 gggcagttcg atgccaaggt tccgatcggt ggccacgaag cgggggcccg cacccgagtg
    29101 caggtcggcc aactcgttgg tgtagtcgcg ctccagaccc caccacagtg gcggacgcag
    29161 tcctgacggc gcctgcccga cctgtaccgg cgtcacccgg tagcgccggt ccggatccag
    29221 gccggggaag gtcaaccggc cgagcatcac tgcgtccgtc ccagccatcg ccgcgaacga
    29281 atagatcgcc tggctgcggt cgggggagac cacaccaccg gccagcagcg cagcgtccgg
    29341 gaagtcgagc cgaaccacgt cgccgcccag cagcagctcg cgatgctcct tgtagaacgc
    29401 aatccactcg gtcagctccg cgagttccgc agcggtggcc gtgttcaggt tccactcgat
    29461 gccgaagtgg ccgaacagcg cggtggccgc ccggaagttg aggtcgtggc tgcgtccggt
    29521 ggtgtgcgag atgccggacg cgacgtgcga acccagcatc tccggcggca ccagctggcc
    29581 ggtccagcgc agcatctgct ggcggtcgtg cgggtcgatg cagtccgaga cccatatccg
    29641 atcggtgcgc tccaggacac ccaggtccac ccggccgccg ccggaagagc aggactcgat
    29701 ctccaggtgc gggaaggccg cccgcagttc gtccagcaac cggtacaccg cggcggtctg
    29761 caggtgcacg cccgggcgcc cggacggctg ggtgccggca tcgatcaggt cgcggttgtg
    29821 gtcccacttg aggtaggcga tgtcgtactc ggtcaggatc gcgagcagcg catcgcggat
    29881 gtggtcgtag cactccggga tcccgaggtt cagcacctgc tggtgccgcg aggccaccgg
    29941 caaccggccg ccggtggcca tcacccattc ggggtgagcc ctggcgaggt cggaatccag
    30001 gttcaccatc tctggctcca cccaaagccc gaactccatg ccgagctccc gcacccggtt
    30061 gaccagcggg tgcagcccgt ccggccacac gtcagcagac accgtccagt cgccgaggcc
    30121 cgagaagtcg tcgcgccgcg aaccgaacca gccgtcatcg agcacgtacc gctccacgcc
    30181 gatggccgcc gccttctcgg ccagctggag cagggtgggc aggtcgtggt tgaagtacac
    30241 cgcctcccag acgttcagcg tcaccggacg cttcgtcccc gggtgctgcg gacgcgagcg
    30301 caggaaccgg tggaagcggc gggccaccgc atccaggccg cggccgtagg cgccgtagag
    30361 ccacggcgtg gcgtagccgt gccccgggcc cagcacgatt tcgccgggca gcagtagctc
    30421 gccgccgccc agcacctggt gcccggtgaa ggtgcgttcg gcgtagtggg tgtggttgcc
    30481 gctccagccg acgtgtacgc cccaaacctc cccgtcgccg aagctgaagc cgggaacgcc
    30541 ggcatggagc acggttgctg cgtccgcacc ggtacggccc ttgcgccctt cgcgcaagtg
    30601 cgtgccgacc gtgaactcgc gacgttgcgg gacgcgttcg cggccccagc ggccggccag
    30661 gtcgagcagc tcgcgagccg tggacggcac cgggaaagcg agcacgaggt cgttcagctg
    30721 gtagtcgtcg gtgccggtgt tggccagctc tgcgcgcgag cgcagcagcc ccgacggcgt
    30781 cagctccagg tgcaggcgca
//
LOCUS       DAOPRQ010000027        30511 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_1178_length_30511_cov_13.185579, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000027 DAOPRQ010000000
VERSION     DAOPRQ010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 30511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30511
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1178_length_30511_cov_13.185579"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..823
                     /locus_tag="PKJ61_05165"
     CDS             <1..823
                     /locus_tag="PKJ61_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13244.1"
                     /translation="AEGVLVGGKAGTGPALLRLTGTTPSESLDLRPAEPAAAEATLIS
                     VSVARDEVSAIGRVFGGAHSNPRLTIWDGTDPPGTLTSRPQEFFTFGGHDAGPLLGIL
                     DVAGEPVIVGSRATTEGGEAVLWTRTGTTWEKHAGLGAAVQSDARRVLGFAAAAQAGT
                     QVLIGGDEVSLAGELQQRPAVWLGSVSGSWQQLTPAVPSALAPVGGQLSRVTSLACSQ
                     LDNCWAAGWTAGRPVAWSVDAAGATVGDPELLPGDAPRDGDPEALVTMLGETPPG"
     gene            948..2651
                     /locus_tag="PKJ61_05170"
     CDS             948..2651
                     /locus_tag="PKJ61_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEDD exonuclease domain-containing protein"
                     /protein_id="HOC13245.1"
                     /translation="MTTTLLQPSFEDLGRPLSDVTFCVVDLETTGSAAEDCITEFGAV
                     KVRGGEVLGEFQTLVNPNAHIPAIVAVLTGITDSMVAGAPKLAEALPSFLEFAAGSVL
                     VAHNARFDVGFLQRACTLHGYPWPDPEVIDTVALARQVLLRDEVPNVKLATLAAHFRT
                     STTPNHRALADARATVDVLHGLLERIGNLGGGTLEDLVEFTRRVSPERRAKRGFAKGL
                     PTAPGVYQFVADLTGPDGATRRQVLYVGKSRNLCRRVASYFTAAESRPRIDEMVRIST
                     GVETTVCRTPLEADVLELRLIAAHSPRYNRRSKFPERQVWLKLTIEAFPRLSIVREIA
                     DDGARYFGPFRRRASAEQVLMAIYDAFPIRQCTARLSARRPTNACALAGMGRCCAPCE
                     LAVTPQEYAAVTEQVLQCLSADVRPLAHAVEPRLNQLVRTQRFEDAAVLTERLDAFSR
                     ATLRHHRLASLAACSQIVAACRSEAGWEVHVVRYGRLAAAALARPGDVPQQVARDAVT
                     TAEWVARPLGPAPAAIVEETERVADWLERPGVRLIEIDGDWCWPVGIGPSVASQIRIS
                     A"
     gene            2648..2926
                     /locus_tag="PKJ61_05175"
     CDS             2648..2926
                     /locus_tag="PKJ61_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HOC13246.1"
                     /translation="MITAIVFVKADVARIPEVAQQIADLDGVSEVYSVTGKIDLIAMV
                     RVRGMDDVATVIADQLNKVPGVQSTETHIAFRAYSTHDLEAAFAIGAE"
     gene            complement(2956..3486)
                     /locus_tag="PKJ61_05180"
     CDS             complement(2956..3486)
                     /locus_tag="PKJ61_05180"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOC13247.1"
                     /translation="MSKKATLHTNHGDIVINLFADQAPVTVDNFVGLATGTKEYTDPS
                     TGEKKTGNFYDGLGFHRVIDGFMIQGGCPLGTGTGDPGYRFDDEVHPELVFNKPYLLA
                     MANAGIQFGRGTNGSQFFITVVPTPHLNRKHTIFGEVADEASRKVVDAIAKVPTGRMD
                     RPIEPVVINSVEITEA"
     gene            3594..>4360
                     /locus_tag="PKJ61_05185"
     CDS             3594..>4360
                     /locus_tag="PKJ61_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HOC13248.1"
                     /translation="MKTKKLLAAVEDGTQTAVEYLTPYVEQALREGGEFADATYTKLR
                     PVLNDARVRGARVAADTFEKVHPSIDDALDRVSPAVEAGVRKVRPAVEEALTQIPPTV
                     DFARSKVQDEFLKNLSKTLAKAASQPLSTEVKAAVATAALAKQLSNTIAPKKRSGWRT
                     FGKILLAGALLGGVVAAIKKLLADPSTGWETHSPRSSYVADPVNPAEEAKTKVSAFAS
                     KAKDVAEDVEDKVKDAADEVVDKAKDVADDLSDQAADV"
     assembly_gap    4361..4460
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4461..4780
                     /locus_tag="PKJ61_05190"
     CDS             <4461..4780
                     /locus_tag="PKJ61_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015011448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13249.1"
                     /translation="DVADKVEDAKDAAKDVAEDVEDKLARLADETDSAEGDDASPLAG
                     SPYGEGSYVGDEPPEGYVIKGNDRSMKYHLPGSAAYERTIAEVWFASEEAAEAAGFVR
                     AQR"
     gene            4865..5125
                     /locus_tag="PKJ61_05195"
     CDS             4865..5125
                     /locus_tag="PKJ61_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13250.1"
                     /translation="MEQNYYWVQAAPFRALLEQMIEATGTPWPELAGRAHVPLRLTHA
                     LLFGQNGRQLPRVPYDFARRIILLAEVMGQVPPGTAARAYAA"
     gene            5128..5811
                     /locus_tag="PKJ61_05200"
     CDS             5128..5811
                     /locus_tag="PKJ61_05200"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HOC13251.1"
                     /translation="MYAVLLALGLAALSAVLAGPLLRWCPVPTEAWTDTDPDLIAANF
                     GALLGPRFRWTVFIASLSAGALAFAVLPGHLWAVWAPLVVLGPVLAVVDLQTTFLPSR
                     IVYLGIALALLGASWAAWAQASWQPLLNAVGGSLSATAFFWLLWRLSGGQLGFGDVRL
                     AGFIGMACGTSGPQAVWQALLLGTLVGAAWGLITRWRRGSDGAFPYGPALLFGPGLWL
                     LLSWLSRLW"
     gene            complement(5786..6832)
                     /gene="trpD"
                     /locus_tag="PKJ61_05205"
     CDS             complement(5786..6832)
                     /gene="trpD"
                     /locus_tag="PKJ61_05205"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014847074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HOC13252.1"
                     /translation="MTFTWPGVLSDLINRVDLSPEAAAWAMDQILSGDTTAVQVAGFA
                     VALRAKGETPAEITGLASAMLAKATPIRLNPEAVDVVGSGGDRANTVNVSTMAAILTA
                     SCGVPVVKHGNRAASSACGTADCLEALGVAIDLPPAAQAGVLAEAGIVFLFASLYHPS
                     LRFVASARRELGVQTTFNFLGPLANPARPVAQAVGVADARMAPLMAQVFAGRGQRGLV
                     FHGADGLDELTTTTTSDVWLVRDGEVVQTSLDPAELGVIPATRSDLIGGTPEHNAQVV
                     RDVLAGRPGAIRDIVALNAAAAQLAFVGPELDAPLAGQLSEPLRRAYAAIDTGAAGRT
                     LDTWVRATRAAKAN"
     gene            complement(6829..7245)
                     /locus_tag="PKJ61_05210"
     CDS             complement(6829..7245)
                     /locus_tag="PKJ61_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014847075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOC13253.1"
                     /translation="MTEDQGTARVLLYSDDRAVREQVRLALGKRVAADLPELEVFEVA
                     TQPAVLQAMDAGGIDLAILDGEAVPSGGMGLCYQLKNEIEDCPPVLLLVARVADAWLA
                     TWSKADAIAAHPLDPVKLPTTVAELLRARPSAEATA"
     gene            complement(7242..8243)
                     /locus_tag="PKJ61_05215"
     CDS             complement(7242..8243)
                     /locus_tag="PKJ61_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020390395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HOC13254.1"
                     /translation="MEVTGPLLPLHADPGNQPPPLDWLRLFIEWKWDWLFAVPASIGL
                     FGYLWLVWRLHRRGDRWPAVRTWSWCVGGIGTAVVAGMSSLGAYDTVLFSVHMVQHMM
                     LTMITPVFLAVGAPVTLLLRNLGKRGRRRAMRVLHSPPLRVLFFPPLATALLITTPFA
                     LYLTELYRYTLDVDWAHDLLHVYMVTVGCLFFFPLLGNDPMPNRLPYPMRILLLFITM
                     PAQAFLGTTIMGSSRLIAEDWYLAFGRAWPPSPIDDQYIAGAIMWATGDLTMGVIMAV
                     LLAQWYSESKREAARIDRQLDREERLAARRASAESPPQNHYDEAVDRAVAEMDGKDQ"
     gene            8304..9035
                     /locus_tag="PKJ61_05220"
     CDS             8304..9035
                     /locus_tag="PKJ61_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-copper oxidase subunit III"
                     /protein_id="HOC13255.1"
                     /translation="MAHTTPLSAPVGTPHGALHPIASSRLQGLRGRPDPLSVGVIVWL
                     ASELMFFAALFAAYFSLREITNAAAAAAGSTPMWEWGAAHLDNDWFGVTFPWFATINT
                     TILILSSVTCQLGVHAAEKGQVGRTGSLLKVGGWGLREWYTLTFIMGAIFIGGQAYEY
                     ANLIGEGFLLSTNAYTSVFFLATGFHGLHVIGGLVAFLLMLGRTYMSRRFTHEQAVHA
                     IVTSYYWHFVDAIWVVLFGVLYLLR"
     gene            9088..9876
                     /locus_tag="PKJ61_05225"
     CDS             9088..9876
                     /locus_tag="PKJ61_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HOC13256.1"
                     /translation="MRRRKAARPVLLLLSLALVGVLYAAITPAAQSAAEGASNEQLVA
                     TGKQLFAVSCASCHGLNGEGFAQQGAPSLAGVGAAAVDFQVRTGRMPMTNPLVQAPRK
                     DNTFTEDEIAAMAAFVASLGDGPGIPTEDQYDPTGVNAEGAARGGEMFRTNCSACHNF
                     KGAGGALPLGVVAPSLQGVEPKEILEAMRTGPGQMPVFASGAIPDENVQEMIAYLQRV
                     NATPSAGLTFGGIGPVAEGLWAWILGIGSLVLFAIWITTRGARS"
     gene            9873..10922
                     /locus_tag="PKJ61_05230"
     CDS             9873..10922
                     /locus_tag="PKJ61_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002513973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HOC13257.1"
                     /translation="MNHHAAVEEHPVTDPGIEQHPPRQSDVDPAVADRAYRRIVGMLM
                     AVPVLVVAFLVVYFAVPRDAELIVFGANLKMQTTLLGLSAGIAVLLVGMAAIVWARSL
                     MTDEEIVDDRHELSSPPEVREQVLADIEQGIEETGIRRRKLIGSGMAGALGLLALPAV
                     VSLADMGPWPTADLRAKTIEKTVWAEAMQLVNDVNHLPLKADEIQVGQLINAQPKALQ
                     DLHGTAYQQAKAKAAIIVVRMDPNTIRIPESRRDWQVDGILCYSKICTHVGCPISLWE
                     QQTHHLLCPCHQSTFDLGNSGVVVFGPAARSLPQLPIMVDKDGYLVARSDFTVPVGPS
                     YFERDSSKDFQDGDR"
     gene            10922..12595
                     /locus_tag="PKJ61_05235"
     CDS             10922..12595
                     /locus_tag="PKJ61_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004811858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase cytochrome b
                     subunit"
                     /protein_id="HOC13258.1"
                     /translation="MAKPAQVSAESPALETHTPPKPEAANPLAGPATWVDDRVGLAKV
                     VGNIRKVFPEHWSFLLGEIALYSFIILLLTGVFLTIWFKPSMAEVEYAGTYELMHGIP
                     MSEAFASALAISFDVRGGLLVRQIHHWSAMLFVAAITVHMLRVFFTGAFRKPRELNWI
                     IGVGLFSMALVEGFAGYSLPDDLLSGTGLRFVDGLIRSIPLVGTWAEFFVFGGEFPGE
                     LVIPRLYMVHILLIPGLLLGLISAHMALLVYHKHTQWPGPGRTQKNIQGYPLFPVYVA
                     KAGGFFFIVFGITILMGALMQINPVWLFGPYNPAQVTAGSQPDWYMGFVEGAIRIIPN
                     WESHLWGTTWSWNIFLPGVGLMGLLFTGLAVYPFIEAWITGDKSEHHLLDRPRNAPNR
                     TAFGVAGMTAYGMFWLSGGNDILATQFHVSLNAVTYFMRVAVFVAPVLAFIITKRICL
                     SLQRADRERVLHGSESGVIVRNPEGGYTEAHVPVSHEEAFNLTRHPQLPAQEVKGKLR
                     GRLSRWFADGQVPKPTKAEIAGAHHPQSPALPGEEDAPALSGSQSEDQH"
     gene            complement(12592..13203)
                     /locus_tag="PKJ61_05240"
     CDS             complement(12592..13203)
                     /locus_tag="PKJ61_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015069822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HOC13259.1"
                     /translation="MTDKIDFKKLLGCYQAGREFAVVEVPEMQYLMIDGSGDPNAGGP
                     FAEAVTALFSIGYPLKFASKRELGRDYTVMPLEGLWSAADMASFTTALDKSTWDWTLL
                     MMVPDWITPGLFAEAASSARDKAGEVVERVRLERLTEGRCVQILHLGPFDAEAETLHR
                     LHTEYLPAHGLTPSGRHHEVYLSDFTRTAPDRLRTILRQPVAG"
     gene            13307..14752
                     /locus_tag="PKJ61_05245"
     CDS             13307..14752
                     /locus_tag="PKJ61_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014847076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GuaB1 family IMP dehydrogenase-related protein"
                     /protein_id="HOC13260.1"
                     /translation="MRFLSGKPEFDLTYDDVFMAPRRSSVTSRLDVDMTSTDGLNLPL
                     PMIVANMTAVAGRRMAETVARRGGIAIIPQDLPTDIVARAIGKVKAAPTVFDTALTID
                     PHATVGEALMLLPKRAHGMVVVVDADGAPIGAVSEAEMAGLDRFAQVHEAMTTDLTLL
                     PADATPTEVFDELSARRHKAALAVRDGKLVGLMTLKGAVRAALYQPALDASGKLKIGA
                     AVGISGDVETRAEALLNAGADVLVMDTAHGHQERMISATERCRAVVDALDRLVPIVAG
                     NVVTADGTADLIEAGADVVKVGVGPGAMCTTRMQTGVGRPQFSAVLECAAEAHDSGKS
                     IWADGGVRHPRDVALALAAGSGAVMIGSWFAGTHESPGELLFASDGRPYKESFGMASA
                     RAVRARTRTQSAFDRARAALFEEGISSSRMYLDSARPGVEDLIDFIVSGVRSSCTYAG
                     ARNLTEFAAQAIVGVQSASGYNEGRPLHSSW"
     gene            complement(14900..15295)
                     /locus_tag="PKJ61_05250"
     CDS             complement(14900..15295)
                     /locus_tag="PKJ61_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002527710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 4"
                     /protein_id="HOC13261.1"
                     /translation="MKAERWIFVFLSIFFLVVVPIYWFMSREVIGTFALLMTFALCAM
                     LAVYLSIQSNKLDARPEDRKDGEIIEGAGEMGFFPSRSIWPFWAALTVTVMLLGPVFG
                     WWITLLGVGMGIWSITGWTYQYYRDEYQH"
     gene            complement(15292..17004)
                     /gene="ctaD"
                     /locus_tag="PKJ61_05255"
     CDS             complement(15292..17004)
                     /gene="ctaD"
                     /locus_tag="PKJ61_05255"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002547344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HOC13262.1"
                     /translation="MTVARMTLDEVEARPLVERKRWGKFAMNLLTTTDHKVIGNMYFV
                     TSLLYFAFAGLLALAIRAELAFPGLQFMHYETFNQFFTMHGTLMLLMFATPMFVAFAN
                     AIMPLQIGAPDVAFPRLNAFSFWLYLFGSLTSCAGFLTPGGAASFGWFAYVPLSDAIN
                     SPGIGGDLWLMGLYVMGLSTILGAVNFITTIMTLRAPGMTMFRMPIFTWNILVTSIMI
                     LICFPVLAAGLLVLEADRRLGTHILDPATGGAILWQHLFWFFGHPEVYIIALPFFGII
                     TEVLPVFSRKPIFGYVGLVAATLAIGALSIAVWAHHMFVTGAVNLPFFSFMTFMIAVP
                     TGVKFFNWIGTMWGGAISFDTPMVWSIGFLTTFLFGGLTGIMLASPPLDFPVSDTYFV
                     VAHFHYVLFGTVVFAMFAGFYFWWPKLTGRMLDERIGKLHFWLLFIGFHLTFLVQHWL
                     GVEGMQRRIADYLPGEGFTFLNQVSTVGAFLLGISFLPFIWNVWISRKSPLVNTDDPW
                     GWGRSLEWATTSPPPRFNFNKLPRIRSEAPAFDLNHPAEAVAAPINPVQEAIDETTQK
                     EVEQ"
     gene            complement(17001..17825)
                     /gene="coxB"
                     /locus_tag="PKJ61_05260"
     CDS             complement(17001..17825)
                     /gene="coxB"
                     /locus_tag="PKJ61_05260"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015069952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HOC13263.1"
                     /translation="MLGAGFAGLLALVLTGCSSETLAQWGRFGLPEAATDRAPFIGNL
                     WVGAWIASLVVGVFVWGLIFWVMVRYRRKHPDQAPRQTRYNLPLELLYTLVPFLIIGV
                     LFFFTVNAQNNVLAKEKEPQRTVNVIGQKWSWTFNYMEAENPAVGTVIHEAGTVDRIP
                     DLYLPVNEAVRFNLVSADVIHSFWIPAFYFKLDVIPGHPNSFDVTPDKLGTYSGKCAE
                     LCGTYHAAMLFTVHVVTAEEYEAYLGRLKAAGQTGEIKAPEYPNTVPSVPAEEEHK"
     gene            complement(17925..18275)
                     /locus_tag="PKJ61_05265"
     CDS             complement(17925..18275)
                     /locus_tag="PKJ61_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017933943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HOC13264.1"
                     /translation="MTVDSQTVTDGIVLTEGAASKVKDLISNEDVDSLALRISVAPGG
                     CSGLRYQLQLDDRQLDGDVVADFFGVSVVTDRMSAPYLSGATIDFVDTIEQQGFTIDN
                     PNAQGSCACGDSFH"
     gene            complement(18373..19323)
                     /locus_tag="PKJ61_05270"
     CDS             complement(18373..19323)
                     /locus_tag="PKJ61_05270"
                     /inference="COORDINATES: protein motif:HMM:NF014634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HOC13265.1"
                     /translation="MTFRVVLAADRIGALDSMTAGVALARGFAANAQVAVVPIAAGGP
                     DLANAFASLVGSEAPAATADRWVVRAPGRILLGLSQPRTPNWRPDATTSHLGEWVAAN
                     VSEEEEVVLDLTGLNAQDGGAGLLAAARSVLEGRRLVGVVAADELALPATGIGGGLAK
                     RAFADRVDVADLLRADAALVAWADRLAPGLATAPGGGAAGTTALAVLALGGELVSGTQ
                     FCHRQAGLGGTLAAADLVVTGCSELSALDRGGPVVSAVTGWAADAQLPCVLFTTGAGL
                     ARRELRTLGLEAAHLLASATPSEADLTAAAARIATGWVST"
     gene            complement(19320..19658)
                     /locus_tag="PKJ61_05275"
     CDS             complement(19320..19658)
                     /locus_tag="PKJ61_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13266.1"
                     /translation="MNELREQAELAYQRALSGLHGARAELADVMAARRRLVFDRTRLD
                     PAVANQREAELEAGRVALESRVGRLRAEAEELRRAVRRMTDASDEPLEVEPGPDADGF
                     EQPPFLQDRP"
     gene            19737..20324
                     /locus_tag="PKJ61_05280"
     CDS             19737..20324
                     /locus_tag="PKJ61_05280"
                     /inference="COORDINATES: protein motif:HMM:NF022686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3043 domain-containing protein"
                     /protein_id="HOC13267.1"
                     /translation="MGLFRPYERSEADQPKPEAASSSASSSAEAGKPAKKEVPTPSRR
                     EAEAARRERLNPTLTPKEAKARERTARAALRDEQFARTEGTPLKVLMRDFVDSHRGVA
                     QYSMPILMVTLAVSLLLTNFSQELAVGVTMFTYAMFLMIALDVFLMWRRFKKLAAERL
                     PGQPLKGMLGYMINRSINLRRLRLPAPRVKPGDEI"
     gene            20324..20527
                     /locus_tag="PKJ61_05285"
     CDS             20324..20527
                     /locus_tag="PKJ61_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13268.1"
                     /translation="MGYYWAATPEPADEVSRDLGLEVEFDSQGEAEAWLTASYAELAD
                     AGVHAVALYESDRLVYGPMSLDA"
     gene            complement(20614..21357)
                     /locus_tag="PKJ61_05290"
     CDS             complement(20614..21357)
                     /locus_tag="PKJ61_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012924324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HOC13269.1"
                     /translation="MTRPVPAASAALRVLRYLSERTTPVPAARIAHDLGLPRSSMYHL
                     LTAMAAESFVVHYPEDQLWGVGVAAWEVGQGYTRQAPLTRLARVPIARLVDSLGLSAH
                     LAVLLGADVVYLVEERATGADRLVTDVGVRLPAHLTASGRAILAGLPPAHVRALYPSG
                     AALVRRTESGPRTLAELRTLLAVTRRRGYAEEDSDVTAGFASVAVGLRSASNLAAVAV
                     TWRTPLAGDPERVLERLRQTATVIEGRLR"
     gene            21436..23049
                     /gene="hutH"
                     /locus_tag="PKJ61_05295"
     CDS             21436..23049
                     /gene="hutH"
                     /locus_tag="PKJ61_05295"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002550469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HOC13270.1"
                     /translation="MEIVLGAVPLTPANVVAVARAGARVRLSSEATAAIDASREHVAA
                     LAATAEGVYGVSTGFGALATRSIPAPERAQLQRSLIRSHAAGNGPLVEREVVRAMMLL
                     RIATLAKGFSGVRLATVEAYLGLLNSGLVPAVHEYGSLGCSGDLAPLAHCALVLMGEG
                     EVLDGSVVRPAAAALAEHGLAPIELAEKEGLALINGTDGMLGQLVLALADLALLLDTA
                     DLAAALSIEGQLGTDRTFAADLMALRPQLGQSLSAANLRAFLAGSPILASHRYGDGVV
                     QDAYSLRCTPQVHGSLRDTADFASRIAERELAAAIDNPSVLADGRVESHGNFHGAPLA
                     HVLDFLAIGVADVASISERRTDRFLDVHRNRGLPPFLADDPGIDSGLMIAQYTQAGIV
                     STLKRLAVPASVDSIPSSALQEDHVSMGWAAARKLRTALDGLARVLAIEILTASRGVE
                     LRAPLRPGVATAAAISVLRETIPGPGPDRFLAPEIEAAYVLVRSGALVAAAHQATDAD
                     PGLRRDDVVEKTVIPGLPRDLPSAPEENR"
     gene            23046..24692
                     /locus_tag="PKJ61_05300"
     CDS             23046..24692
                     /locus_tag="PKJ61_05300"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008358364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HOC13271.1"
                     /translation="MNHEPVRAPRGTALSAKSWQTEAPLRMLMNNLDPEVAERPEDLV
                     VYGGTGRAARSWEAFDAIVASLRDLADDETLLVASGKPVGIFRTHEWAPRVLIANSNL
                     VGDWATWPEFRKLEAAGLTMYGQMTAGSWIYIGTQGILQGTFETFGAVARKRFGGTLA
                     GTLTLTAGCGGMGGAQPLAVTMNEGAVLIVDVDASRLARRVEHGYLDEFTSDYAEAVA
                     KVLAAKAERRALSVGLVGNAAEVFPRLLAEGVAVDVVTDQTSAHDPLAYLPVGVSVDE
                     WRELAATDPEGFTERARASMAAHVEAMLGFQDRGAEVFDYGNSLRAEAQLGGCERAFD
                     FPGFVPAYIRPLFCEGKGPFRWAALSGDPADIAVTDAAILDLFPDDAHLRRWLTAASE
                     KVHFEGLPARICWLGYGERHLAGLKFNELVASGAVSAPIVIGRDHLDSGSVASPYRET
                     EAMADGSDAIADWPLLNALLNTASGATWVSIHHGGGVGIGRSIHAGQVVVADGTDLAA
                     QKIARVLTNDPGTGVMRHVDAGYERAREVARERGVRIPML"
     gene            24692..25891
                     /locus_tag="PKJ61_05305"
     CDS             24692..25891
                     /locus_tag="PKJ61_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012924315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016813 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear
                     amidines [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate amidohydrolase"
                     /protein_id="HOC13272.1"
                     /translation="MSGANELLAEVAGIGRESGGGYTRLSWDAATMELREWFAGQADA
                     LGLDLVVDGNGNQVAWWGSGTGALLLGSHLDSVPNGGPLDGPLGVTSALAAVAALQRE
                     GWRPARPIAVANFTDEEGGRFGVACLGSRLVTGAIATETALGLRDADGNTLAEVMAAR
                     GFAPEAAGVADWLDQVSSYIELHVEQGRWLAPAAAPVGVATAIWPHGRYRFDFCGQGN
                     HAGATAMADRHDPMLAYAATVLAARELASATDQRATLGRVRIEPNSTNSIPSRVTAWL
                     DARASSEAGLEALVAAIVSKAQLAAEVDGTSVEVRAESVSGAVEFDHALRHRLAALLD
                     APVLPTGAGHDAGILAAKLPTAMLFVRNPTGVSHAPEEFATPEDIDAGVAALIQVVRE
                     VGSEAAR"
     gene            25888..27015
                     /locus_tag="PKJ61_05310"
     CDS             25888..27015
                     /locus_tag="PKJ61_05310"
                     /EC_number="3.5.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020390211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamate deiminase"
                     /protein_id="HOC13273.1"
                     /translation="MSGATYPAFANCHSHAFHRALRGRAGGSDFWGWREGMYRVAQVL
                     DPDLYYRLARATYAEMALAGIGAVGEFHYLHHDRGGVRYAEPNAMANAVIAAAWEVGL
                     RICLLDTCYLRVGFDDAEPAAAQLRFFHADAAEWAAGATDLAEKYADNPAVVIGAAIH
                     SVRAVDEASLPIVASALPFAPLHVHVSEQPAENAACMAATGRNPVELLADAGVWTPRT
                     TAVHATHLTDADIATLGAAGGFVCFCPTTEAELADGIGPSIALRAAGARITLGSDSNT
                     VIDLFAEARGLALHERLASGFRDGWSAAKLWQAATTDGAASLGFEGLGGFELTESVRT
                     AGAGEPIWAASAADVKPAPDLDPGWVTAELQAAIDEVWSRT"
     gene            27012..28208
                     /gene="hutI"
                     /locus_tag="PKJ61_05315"
     CDS             27012..28208
                     /gene="hutI"
                     /locus_tag="PKJ61_05315"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017935941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HOC13274.1"
                     /translation="MSAASLAITSIGELTTWDDERPIRHDAGLVIEAGRVVWVGASGD
                     TPSADRRIDAQGGAVIPGFVDSHTHLVFAGDRAEEFAARMAGKPYAAGGIRTTVAATR
                     SATEPELERLARHRLAELRAQGTTTVEIKSGYGLDVATEERLLRVASALTEETTFLGA
                     HVVPSGWEANAYVELVTGEMLARCAPFAKWVDVFCERGAIDADQARLVLSAGIAAGLQ
                     PRVHANQLGPGPGVQLACELGAAAADHCTYLSDSDVSALAETGVVAGLLPGVEFSTRH
                     PYPDARRLLDAGVRVAIATDCNPGTSYTSSMPFCIALAVREMGMTPTEALHAATQGGA
                     GSLRREDVGHLRLGARADLAFLDATSHVHLAYRPGVPLVKRVLVGGDAWPGLSPRGRR
                     APRTGA"
     gene            complement(28168..29262)
                     /gene="gcvT"
                     /locus_tag="PKJ61_05320"
     CDS             complement(28168..29262)
                     /gene="gcvT"
                     /locus_tag="PKJ61_05320"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008357348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HOC13275.1"
                     /translation="MTDPLKSPLHERHEALGAKFSEFGGWLMPLQYSGVVAEHRAVRN
                     AVGVFDVSHLGKVRVLGTGAAEFVNSCLTNDLGRIQPGQAQYTLACNDAGGAIDDIFT
                     YLISPDEVFLVPNAANNATVYGLLSAAAPAGIEVRNEHRDHAVIAVQGTNSDETLAAL
                     GLPVGHDYLTFLMGSYGGVDLIVCRTGYTGERGYELIVPASAAVQVWDAVLAAGREYD
                     ILTCGLGARDTLRTEMGYPLHGHDLSPEISPVMANLNWAVGWTKPTFWGADALRAQRE
                     AKAVPLLRGLKALGRGIPRPHMAVQAGGVGVGEVTSGTFSPTLGAGIALALLDRSVAV
                     GDQVDVLVRDRLEPFEVVKPPFVEPGVREG"
     gene            complement(29290..30426)
                     /locus_tag="PKJ61_05325"
     CDS             complement(29290..30426)
                     /locus_tag="PKJ61_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015069293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOC13276.1"
                     /translation="MISFNFATAGRIAFGAGRSSELLDVVASLGRRPFLCTGSDASRY
                     LSVYETLADYATFAVAGEPTMDVVRAGAKAAREHGADVVIGLGGGAVLDAAKVIAALV
                     TNGGDPLDYAEVVGAGRPLRVASLPVVALPTTSGTGSEVTANGVVTSTEHRVKVSLRS
                     TSMLPRVAIVDPELTLTCPPATTAHAGLDALVQCIEPFVSPFATPLTDGFCREGIRRA
                     ASGLQRAYHHPDDLDARTDVSVCSLLSGLALANGKLGAAHGLAGPLGGFLGAPHGAIT
                     ASVMVAVSEHNLAVLKASDDRQGLARYAEVGELLTGRREAAAFLGWFTDTAAELGVPG
                     LAALGLADADVDGIAVAATNASSSKGNPVPMTPEAFAAVLRRSM"
BASE COUNT         4833 a        10358 c        10483 g         4737 t
ORIGIN      
        1 cgccgaaggc gtcctcgtcg ggggcaaggc cgggactggt ccggcactgt tgcggttgac
       61 cgggaccacg ccctcggaga gccttgacct gcgaccggcc gagcctgccg ccgcggaagc
      121 gaccctgatc tcggtcagcg tggctcgcga tgaggtgtcc gccatcggtc gagtcttcgg
      181 cggtgcgcat tcgaacccac gcctcacgat ctgggacggg accgacccgc cgggcacctt
      241 gaccagccgg ccgcaggagt tcttcacctt tggcggccat gacgccggtc cactgctcgg
      301 catcctggac gtggccggcg aaccggtgat cgtgggcagc cgggccacga ccgagggcgg
      361 cgaggccgtg ctctggaccc gcaccggcac cacctgggag aagcacgccg ggctcggcgc
      421 cgcggtgcag tcggacgctc gacgggtcct cgggttcgcc gcggctgccc aagccggcac
      481 gcaggtcctc atcggcggcg acgaggtgag cctggccggc gaactccagc agcgaccagc
      541 agtgtggctc gggtcggtgt caggcagttg gcagcaactc acgcccgcgg tcccgtccgc
      601 gctggctccg gtcggcggcc aactctcccg agtgacgtcg ctcgcctgct cacagcttga
      661 caactgctgg gcggcgggct ggacggctgg tcgccccgtg gcctggtcgg ttgacgcagc
      721 cggcgccacc gtgggcgacc cggagctctt gcccggcgac gccccgcgcg acggtgaccc
      781 ggaggcgctg gtgacgatgc tcggcgagac gccccccggc tgagccggtc accgcggtcg
      841 ctgcgtcacc gtccggcctg tacgccttga ccgcctccgg actgtggtgg cgggaaacgc
      901 cagcctgctg acggaagttg tcagtggcac ggcctaacgt gctgttcgtg accacgaccc
      961 tgctgcagcc gagcttcgaa gaccttggcc gaccgctgtc ggacgtcacg ttctgcgtgg
     1021 tcgacctgga gaccaccggc agcgccgccg aggactgcat caccgagttc ggcgcggtca
     1081 aggtgcgtgg cggcgaggtg ctcggcgagt tccagaccct ggtgaacccg aacgcacaca
     1141 tccccgcgat cgtcgccgtg ctaaccggca tcaccgactc catggtcgcc ggggctccca
     1201 agctggccga ggcgctgccg agcttcctcg agttcgcggc cggtagcgtc ctggtcgcgc
     1261 acaacgcccg cttcgacgtc ggtttcctgc agcgcgcctg cacgctgcac ggctacccgt
     1321 ggcccgaccc ggaagtgatc gacacggtcg cgctcgcccg gcaggtgctg ctgcgcgacg
     1381 aggtgccgaa cgtcaagctc gccacgctcg ctgcgcactt ccgcaccagc accacgccca
     1441 accaccgcgc tttggcggac gcacgcgcaa ccgtcgacgt actgcacgga ctcctggagc
     1501 ggattggcaa tctcggtggt ggcacgctcg aagacctggt cgagttcacc cgccgggtct
     1561 cgcccgaacg ccgggccaaa cgtggcttcg ccaaggggct accgactgca ccgggcgtct
     1621 atcagttcgt cgccgacctc accgggccgg acggagccac ccgcaggcag gtgctctacg
     1681 tcggcaagag ccgcaacctg tgccgccgag tggccagcta tttcaccgcc gccgagagcc
     1741 gtccgcggat cgacgagatg gtgcgcatct cgaccggagt ggagaccacg gtctgccgca
     1801 cgccgctgga agcggacgtc ctcgagcttc ggctgatcgc cgcgcattcg ccgcggtaca
     1861 accggcgatc caagttcccc gagcgccagg tgtggctgaa gctcaccatc gaggcgttcc
     1921 cacggctgtc gatcgtccgg gagatcgcgg acgacggcgc ccgctacttc ggcccgttcc
     1981 gtcgccgagc cagtgccgag caggtgctga tggccatcta cgacgccttt ccgatccggc
     2041 agtgcaccgc ccgattatcg gcacgaagac cgacgaacgc ctgcgcgctg gcgggcatgg
     2101 gccgctgctg cgcgccgtgt gaactggccg tcaccccgca ggagtacgcg gccgtcaccg
     2161 agcaggtgtt gcagtgtctc tccgctgacg tgcgtccgct ggcgcacgcg gtggaaccca
     2221 ggctgaacca gttggttcga acgcaacgat tcgaggacgc ggcggtgctc accgagcggc
     2281 tggacgcgtt cagccgggcc accctgcgcc accaccggct ggcgagcctt gccgcttgtt
     2341 cccagatcgt ggccgcgtgc cgcagcgagg ccggctggga ggtgcatgtg gtccgctacg
     2401 gccgcctggc ggcagccgcg ctggcccgtc ccggcgatgt accgcagcag gtggcgcgcg
     2461 acgcggtcac gaccgcggag tgggtggcgc gtccgctcgg cccggcgccg gccgcgatcg
     2521 tggaggagac cgagcgcgtg gccgactggc tggagcgtcc cggcgtccgc ctgatcgaga
     2581 tcgacggcga ctggtgttgg ccggtcggca tcggacccag cgtcgcttcg cagattagga
     2641 tcagcgcatg atcaccgcga ttgtgttcgt caaggcagat gtggcccgca ttcccgaggt
     2701 ggcccagcag atcgccgact tggacggcgt gtccgaggtg tattcggtga cgggcaagat
     2761 cgacctgatc gcgatggtcc gcgtccgcgg tatggacgat gtggccacgg tcatcgccga
     2821 tcaactgaac aaggtgcccg gtgtccagag cactgagacc cacatagcgt tccgcgccta
     2881 ctcgacccac gacctggaag ccgcgttcgc catcggcgcc gagtgaacga aaaggggacg
     2941 gttccagata ggcattcagg cttcggtgat ctccacggag ttgatcacga ccggctcgat
     3001 cggacggtcc atgcgtccgg tcggcacctt cgcgatcgcg tccacgacct tgcgggacgc
     3061 ctcgtcggcg acctcaccga agatggtgtg cttgcggttg aggtgcggag tgggcacgac
     3121 ggtgatgaag aactgcgagc cgttggtgcc gcggccgaac tggatgccgg cgttggccat
     3181 cgccagcagg tagggcttgt tgaagaccag ttcggggtgg acctcgtcgt cgaaccggta
     3241 gcccgggtcg ccggtgccgg tgccgagcgg acagccgccc tggatcatga agccatcgat
     3301 gacccgatgg aagccgagtc cgtcgtagaa gttgccggtc ttcttttcac cggtgctcgg
     3361 atcggtgtac tccttggtgc cggtggccag accgacgaag ttatccacgg tcaccggcgc
     3421 ctggtcggcg aaaaggttga tcacgatgtc gccgtgattg gtgtgcaggg ttgccttctt
     3481 ggacatgggg aaccttcctt cacaggtatc agccacccat cctgacagat gcaccctgaa
     3541 ccggcgcagc tacgggtacg gtgggagcat cagagcttcg agcaggaggc acagtgaaga
     3601 ccaagaagtt gctagcggcg gtcgaggacg gcacgcagac cgccgtcgag tacctgacgc
     3661 cttacgttga gcaggccctc cgcgagggtg gtgagttcgc tgacgcgacc tacaccaagc
     3721 tccgtccggt gctgaacgac gctcgtgtac gcggcgcccg agtggccgcg gatactttcg
     3781 agaaggtcca tccgagcatc gacgatgcgc tcgaccgggt gtcccccgcc gtcgaggccg
     3841 gcgtccgcaa ggtgcgtccc gcagtggagg aagcactgac gcagattccg ccgacggtgg
     3901 acttcgcgcg cagcaaggtg caggacgagt tcctgaagaa cctgagcaag accctcgcca
     3961 aggcggcgtc acagccgctg tcgaccgagg tcaaggctgc ggtggcgacg gcagcgctgg
     4021 caaagcagct ctcgaacacc atcgcaccga agaagcggtc aggttggcgg acgttcggca
     4081 agatcctgct ggccggcgca ctgctgggcg gcgtggtcgc ggcgatcaag aagctgctcg
     4141 ccgacccgtc caccggctgg gagacccatt cacctcgcag ttcctacgtc gcggatccgg
     4201 tgaacccggc cgaagaggcc aagaccaagg tgagcgcgtt cgccagcaag gccaaggacg
     4261 tggccgagga cgtagaggac aaggtgaagg acgccgctga cgaggtggtg gacaaggcca
     4321 aggatgtggc cgatgacctg tcggaccagg ccgcggacgt nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn cggacgtcgc cgacaaggtt gaggacgcca aggacgccgc
     4501 gaaggatgtg gccgaggacg tagaggacaa gctcgccagg cttgccgacg agaccgactc
     4561 cgccgaagga gacgatgcct cccctttagc aggtagcccg tacggcgagg gctcgtacgt
     4621 gggtgatgag ccgccggagg gctatgtgat caagggcaac gataggtcga tgaagtacca
     4681 cctgcccggc agcgccgctt acgagcgcac gatcgccgag gtctggttcg cctctgagga
     4741 ggccgccgag gctgcggggt tcgtgcgcgc gcagcgttga aactactgga ccggttcctg
     4801 aagaagtgct gagaatccgg ggtgccgact cacaatcggc ccccggattt cgacttattc
     4861 gggtgtggaa cagaactact actgggtgca ggctgcgccg tttcgcgccc tgcttgagca
     4921 aatgatcgag gccaccggaa cgccgtggcc cgaactggcc ggacgcgcac acgtgccgct
     4981 gcggctgacc cacgccctgc tcttcggtca gaacggacgc caactgccgc gggttccgta
     5041 cgatttcgcc cggcggatca tcctgctggc cgaggtgatg ggccaagtcc cacccgggac
     5101 ggccgcgcga gcctacgctg cttgatcatg tacgccgtcc tgctggctct gggcctggcc
     5161 gcgctgagcg ccgtgctggc cgggccgctg ctgcgctggt gtccggtgcc gacggaggcc
     5221 tggacggata cggatccgga tctcatcgcc gccaacttcg gcgcgctcct cggccccaga
     5281 ttccgctgga cggtgttcat cgcttcgctg tcggcggggg cactcgcctt cgcggtgctg
     5341 ccgggacatc tctgggcggt ctgggcgccg ctggtggtgc tgggcccggt gttggcggtg
     5401 gtcgatctgc agaccacgtt cctgccctca cggatcgtct atctggggat cgcgctggcc
     5461 ctgctcggcg cctcctgggc ggcttgggcg caggcgtcct ggcagccgct gctcaacgct
     5521 gtgggtggca gcctgtccgc cacggccttc ttctggctgc tctggcgact gagcggcggt
     5581 cagttggggt tcggggacgt ccggctggcc ggcttcatcg gcatggcctg cggcacctcc
     5641 gggccacagg ccgtctggca ggcgctgctg ttgggcacgc tggtcggcgc ggcctggggg
     5701 ctgatcacgc gctggcgccg cggaagcgac ggcgcctttc cttacggccc ggcgctcctg
     5761 ttcggcccag ggctctggct gctgctcagt tggctttcgc ggctctggta gcccgcaccc
     5821 aggtgtccag cgtgcgcccg gcggcaccgg tgtcgatggc tgcgtaagcc cgccgcaacg
     5881 gctcactcag ctgtcccgcc agcggcgcgt ccagttccgg gccgacgaac gccagctggg
     5941 ccgcggcagc gttcagcgcc acgatgtctc ggatggctcc tgggcggccg gccaacacgt
     6001 cgcgcaccac ctgggcgttg tgctctgggg tgccgccgat cagatcgctc cgggtggcgg
     6061 ggatgactcc gagttcggcg gggtcgagcg aagtctgcac aacctcgccg tcgcgcacca
     6121 accagacgtc agaggtggtc gtggtggtca gttcgtccag accatcggcg ccgtggaaca
     6181 ccagcccacg ctggccgcgc ccggcgaaga cttgggccat caggggcgcc atccgtgcgt
     6241 ccgcgacgcc cacggcctgc gccaccgggc gggccgggtt ggccagcggc ccgaggaagt
     6301 tgaacgtggt ctgcacgccc agctcgcggc gtgcggacgc gacgaagcgc agcgacgggt
     6361 ggtagagcga ggcgaacagg aacacgatgc cggcttcggc cagcacaccg gcttgagcgg
     6421 ccggcggcag gtcgatcgcg accccgagcg cctccaggca gtccgcggta ccgcaagcgc
     6481 tggacgccgc acggttgccg tgcttcacca ccggcacgcc gcagctcgcc gtcaggatcg
     6541 ccgccatcgt ggaaacgttc accgtgttcg cgcggtcacc accggaaccg acgacgtcca
     6601 cagcctccgg gttcagccgg atcggggtcg ccttcgccag catcgcggac gcgagcccgg
     6661 tgatctccgc cggtgtttcg cccttggccc gcagcgccac cgcgaaaccg gccacctgca
     6721 cggcggtggt gtcgccggag aggatctggt ccatcgccca ggccgcggcc tccgggctca
     6781 ggtcgacccg gttgatcagg tcggacagca cgcccggcca agtgaaagtc acgcggtcgc
     6841 ctcagcgctc gggcgggccc gcagcaactc ggccacggtc gtcggcagct tcaccggatc
     6901 gagcgggtgg gccgcgatcg cgtccgcctt cgaccaagtg gccagccagg cgtctgccac
     6961 ccgcgcgacc agcagcagca ccggcgggca gtcctcgatc tcgttcttca actgatagca
     7021 cagccccatg ccacccgagg gcaccgcttc gccgtcaagg atcgccagat cgatgccgcc
     7081 ggcgtccatc gcctgcagca ctgcgggctg ggtcgcgacc tcgaacactt ccagttcggg
     7141 caggtccgcg gccacccgct tgccaagggc gagacgcacc tgctcacgca ccgctcggtc
     7201 gtcggagtag agcagcaccc gggcagtgcc ctgatcttcg gtcattggtc tttcccatcc
     7261 atttccgcta ccgcgcgatc gactgcttca tcgtaatggt tctgcggtgg cgattcggcg
     7321 ctcgcccgcc gggccgccag ccgttcctcc cggtccagtt gccgatcgat ccgggcggcc
     7381 tcccgcttgc tttcggagta ccactgcgcc agcagtaccg ccatgatcac gcccatggtg
     7441 aggtcgccgg tggcccacat gatcgcgccg gcaatgtact ggtcgtcgat cgggctgggc
     7501 ggccaggcgc ggccgaaggc caggtaccag tcctctgcga tcagccgcga ggaccccatg
     7561 atcgtcgtgc cgaggaaggc ctgggcgggc atcgtgatga acagcagcag gatccgcatc
     7621 gggtagggca gccggttcgg catcgggtcg ttgccgagca aggggaagaa gaacaggcag
     7681 ccaaccgtga ccatgtagac gtgcagcagg tcgtgcgccc agtccacatc cagcgtgtag
     7741 cggtacagct cggtgagata gagcgcgaac ggcgtggtga tcaacagcgc ggtggccaac
     7801 ggtgggaaga acagcacccg cagcggcggt gagtgcagca cccgcatcgc gcggcggcgt
     7861 ccgcgcttac cgagattgcg cagcagcagg gtcaccggcg cgcccactgc gaggaacacc
     7921 ggcgtaatca tcgtgagcat catgtgctgc accatgtgca cgctgaacag cacggtgtcg
     7981 taggccccga gagaactcat gccggccacg actgcggtgc cgatcccgcc aacgcaccac
     8041 gaccaagtgc ggacggccgg ccagcggtca ccgcgtcggt gcagccgcca gaccagccac
     8101 agatacccga acaagccgat gctggccggg acggcgaaca gccagtccca cttccattcg
     8161 atgaataacc gcagccagtc cagcggcggt ggctggttgc ccgggtcagc gtgcagcggc
     8221 agcagcggcc cggtgacttc cataccggac attatcggcg cagatgggtg cccctcgggc
     8281 accggattgg acataatggc gcaatggccc acaccacgcc gctgagtgct cccgtcggta
     8341 ccccgcacgg cgcgctgcat ccgatcgcat ccagccggtt gcagggtctg cgcggccgtc
     8401 cggatccgct atcggtaggc gtgatcgtgt ggctggccag cgagctgatg ttcttcgccg
     8461 cgctgttcgc cgcctacttc tcgttacgcg agatcacgaa cgcggcagcg gccgccgccg
     8521 ggtccacccc aatgtgggaa tggggtgctg cgcacctcga caacgactgg ttcggcgtca
     8581 ccttcccgtg gttcgccacc atcaacacca cgatcctgat cctctcgtcg gtcacctgcc
     8641 agctcggcgt ccatgccgcg gagaagggcc aggtcggacg caccggctcg ctgctgaagg
     8701 tcggcgggtg gggcctgcgc gagtggtaca cgctgacttt catcatgggc gcgatcttca
     8761 tcggcgggca ggcgtacgag tacgcgaacc tgatcggtga gggcttcctg ctttcaacaa
     8821 acgcctacac gtcggtgttt ttcctcgcca cgggcttcca cggcctgcac gtgatcggcg
     8881 gcttggtggc atttctactt atgctcggcc gcacctacat gtcccgccgg ttcacgcacg
     8941 aacaggccgt tcacgccatt gttacctcgt actactggca cttcgtggac gcgatctggg
     9001 tcgtgctgtt cggtgtcctt tacctgctgc gctgaaaact gactcctgaa ggaggaaccc
     9061 gtgaagagag cctcgggaag tcggccgatg cgacggcgta aggcggccag accggtcctg
     9121 ctgctgctca gccttgccct ggtgggcgtc ctttacgccg ccatcacgcc ggcggcgcag
     9181 tcggccgccg aaggcgcgtc gaacgagcaa ctggtcgcga ccggcaaaca gctgttcgcg
     9241 gtgtcctgcg ccagctgcca cggcttgaac ggggagggtt tcgcccagca gggtgcgccc
     9301 agcctggccg gtgtcggcgc cgcggcggtc gacttccagg tgcgcaccgg acgcatgccc
     9361 atgaccaacc cgctggtgca ggctccccgc aaggacaaca ccttcaccga ggacgagatc
     9421 gccgccatgg ccgccttcgt ggccagcctc ggtgacggcc ccgggatacc caccgaggac
     9481 cagtacgacc cgaccggcgt aaatgctgag ggcgcggccc gcggtggcga gatgttccgg
     9541 acgaactgct cggcctgcca caacttcaag ggtgccggcg gcgcactccc gctcggcgtg
     9601 gtcgcgccga gtctgcaggg cgtcgagccc aaggaaatct tggaggccat gcgcaccggc
     9661 cccggccaga tgcccgtctt cgcgtccgga gcgatccccg acgagaacgt ccaggagatg
     9721 atcgcctacc tgcagcgagt gaacgcgacc ccgtccgctg ggctcacgtt cggtggcatc
     9781 ggcccggtgg ccgaaggcct ctgggcctgg atcctcggca tcggttcgct ggtgctgttc
     9841 gcgatctgga tcaccaccag aggggcgcgc tcatgaacca ccacgcagcc gtcgaagagc
     9901 acccggtgac cgacccgggc atcgagcagc atccgccccg ccagagcgat gtcgaccccg
     9961 cggtcgccga tcgggcctac cgccggatcg tcggcatgct gatggccgtc ccggtgcttg
    10021 tggtcgcctt cctggtggtc tacttcgccg tgccgaggga cgccgaactg atcgtcttcg
    10081 gcgcgaacct caagatgcag accaccctgc tgggtctcag cgccggcatc gcggtgctgc
    10141 tggtcgggat ggccgcgatc gtctgggcgc ggtcgctgat gaccgatgag gagatcgtcg
    10201 acgaccggca cgagctgtcc tcgccgccgg aggtgcgcga gcaggtgctc gccgacatcg
    10261 agcagggcat cgaggagacc ggcatccgcc ggcgcaagct gatcggttcc ggcatggctg
    10321 gcgcgctggg cctgctggcg ctaccggccg tggtcagcct cgcggacatg gggccgtggc
    10381 cgacggcgga ccttcgcgcc aagacgatcg agaagaccgt ctgggccgag gccatgcagc
    10441 tggtcaacga cgtgaaccac ctgccgttga aggcggacga gattcaggtc ggtcagctga
    10501 tcaacgcaca gcccaaggcg ctgcaggacc tgcacggcac cgcgtaccag caggccaagg
    10561 cgaaggccgc gatcatcgtc gtccggatgg acccgaacac gatccgcatt cccgagtcgc
    10621 ggcgcgactg gcaggtggac ggcatcctct gctactccaa gatctgcacc cacgtgggct
    10681 gcccgatcag cctgtgggag cagcagacgc accatctgct ctgcccctgc caccagtcca
    10741 cgttcgacct cggcaactcg ggagtagtgg tcttcggccc ggcggcgcgg tcgctgccgc
    10801 agctgccgat catggtcgat aaggacggct acctggtcgc gcgcagcgac ttcactgtcc
    10861 cggtcggacc gagctacttc gaacgtgact cttccaagga cttccaggac ggtgatcggt
    10921 aatggccaag cctgcacagg tgagcgccga gtccccggcc ctcgagaccc acaccccgcc
    10981 gaagccggaa gcggcgaacc cgctcgccgg cccggccacc tgggttgacg atcgcgtcgg
    11041 cctggccaag gtggtcggca acatccgcaa ggtgttcccg gagcactggt cgttcctgct
    11101 gggcgagatc gcgctctact cgttcatcat cctgctgctc accggcgtct tcctgaccat
    11161 ctggttcaag ccgtcgatgg ccgaggtcga gtacgccggc acctacgagt tgatgcacgg
    11221 catcccgatg tcggaggcgt tcgcgtccgc gctggcgatc agcttcgacg tccgcggcgg
    11281 cctgctggtg cgccagatcc accactggtc ggcaatgctg ttcgtggcgg cgatcaccgt
    11341 ccacatgctg cgggtgttct tcaccggcgc gttccgcaag ccccgtgagc tgaactggat
    11401 tatcggcgtc ggcctgttct cgatggcgct ggtcgagggc ttcgccggct actcgctgcc
    11461 cgacgacctg ctctcgggca ccggcctgcg gttcgtggac ggcctgattc gctcgatccc
    11521 gctggtcggc acctgggcgg agttcttcgt cttcggcggc gagttccccg gcgaactggt
    11581 gatcccgcgg ctgtacatgg tgcacatcct gctgatcccc ggactgctgc tcggcctgat
    11641 cagcgcccac atggcgctgc tggtctacca caagcacacc cagtggcctg gcccgggtcg
    11701 cacccagaag aacatccagg gctacccgct gttcccggtg tacgtggcga aggccggcgg
    11761 cttcttcttc atcgtgttcg gcatcacgat cctgatgggc gcgctgatgc agatcaaccc
    11821 ggtgtggctg ttcgggccgt acaacccagc gcaggtgacc gcgggctcgc agcccgactg
    11881 gtacatgggc ttcgtcgaag gggcgatccg gatcatcccg aactgggagt cgcacctttg
    11941 gggtaccacc tggtcgtgga acatcttcct gccgggcgtc ggcttgatgg gtctgctgtt
    12001 caccggcttg gcggtctacc cgttcatcga ggcgtggatc accggcgaca agagtgagca
    12061 ccacctgctc gaccgtccgc gcaacgcgcc gaatcggacg gcgttcggcg tggccggaat
    12121 gaccgcgtac ggcatgttct ggctttccgg cggcaacgac atcctggcca cccaattcca
    12181 cgtctcgctg aacgcggtga cctacttcat gcgggtcgcg gtgttcgtag cgccggtgct
    12241 ggcgttcatc atcaccaagc ggatctgcct ctcgctgcag cgggccgacc gcgagcgggt
    12301 gctgcacggg tcggagtctg gcgtgatcgt ccgcaacccc gaaggtgggt acacggaggc
    12361 gcatgtgccg gtctcgcacg aggaggcgtt caacctcacc aggcacccgc agctgccggc
    12421 ccaggaggtc aagggcaagc tgcgcggacg gctgtcgcgg tggttcgcgg acggccaggt
    12481 gccgaagccc accaaggcgg agatcgcggg tgctcaccat ccgcagagcc ctgccttacc
    12541 cggcgaggag gacgcgccag cgctgtcagg cagccagtct gaggaccagc actaaccagc
    12601 gacgggctgg cgcaggatgg tgcgcagccg gtccggggcg gtgcgggtga agtcggagag
    12661 gtagacctcg tggtggcgcc cggacggggt taatccatgg gctggcagat actcggtgtg
    12721 gagacggtgc agcgtctctg cttcggcatc gaaggggccc agatgcagga tctggacgca
    12781 tcgcccctct gtcagtcgct ccaggcggac gcgttcgacg acctcgcccg ccttgtcccg
    12841 agcagaggaa gcagcctcgg cgaacagccc aggagtgatc cagtcgggca ccatcatcag
    12901 cagcgtccag tcccaggtgg acttgtccag cgcggtggtg aagctggcca tgtcagcagc
    12961 ggaccacaag ccctccagcg gcatcaccgt gtagtcgcgt ccgagttcgc gcttactggc
    13021 gaacttgagc ggatagccga tgctgaacag cgcggtgact gcttcggcga agggcccacc
    13081 tgcgttgggg tcaccgctgc cgtcgatcat caggtactgc atttcgggca cctctacgac
    13141 cgcgaactcc cgtccggcct ggtagcagcc cagcagcttc ttgaagtcga tcttgtcggt
    13201 catgcgtcca gcctcggttc agacgtctcg aatcggcaac accctgacat ggtggcagag
    13261 tccagccacc cgtccgtctg aacgggggtg ccggtaggct tggcgggtgc gattcctgtc
    13321 tggcaagccc gagttcgacc tgacctacga cgatgtcttc atggcgccgc ggcgctcctc
    13381 ggtgacgtca cgcctcgacg tcgacatgac cagcacggac ggcctgaacc tgccgctgcc
    13441 gatgatcgtg gccaacatga ccgcggtcgc cggacgccgg atggcggaaa ccgtcgctcg
    13501 gcgcggcggc atcgcgatca tcccccagga cctgcccacc gacatcgtcg cgcgggcgat
    13561 cggcaaggtg aaggctgcgc cgaccgtctt cgacaccgcg ctgaccatcg atccgcacgc
    13621 caccgtcggc gaggcgctga tgctgctgcc caagcgcgcg cacgggatgg tcgtcgtggt
    13681 ggacgcggac ggcgccccga tcggagccgt ctccgaggcc gaaatggccg ggctcgaccg
    13741 cttcgcccag gtgcacgaag cgatgaccac cgacctcacc ctgctgcccg ctgacgccac
    13801 gcccaccgag gtgttcgacg agctctccgc gcggcggcac aaggctgccc tggcggtgcg
    13861 ggacggcaag ctggtcggcc tgatgaccct gaagggtgcg gtgcgcgcag cgctctacca
    13921 gccggctctg gacgcgtccg gcaagctcaa gatcggcgcc gcggtgggca tctccggcga
    13981 tgtcgagacc cgtgcggagg cgctgctgaa cgcgggggcc gacgtgctcg tcatggacac
    14041 tgcgcacggc caccaagaac ggatgatcag cgccaccgag cggtgtcgcg ccgtggtgga
    14101 cgcgctcgac cggttggtgc cgatcgtggc cggcaacgtg gtcacggcgg acggcaccgc
    14161 cgacctgatc gaggccggag ccgacgtggt gaaggtcggc gtcggcccgg gcgcgatgtg
    14221 caccacccgg atgcagaccg gggttgggcg tccgcagttc tcggcggtgc tggagtgcgc
    14281 ggccgaggcc cacgattcgg gcaagagcat ctgggcggac ggcggcgtcc ggcatccccg
    14341 tgacgtggcc ctggcgctcg cggccggatc gggtgcggtg atgatcggtt cgtggttcgc
    14401 cggcacgcac gagtcgccgg gtgagctgct gttcgcgtcc gacggccggc cgtacaagga
    14461 gtcgttcggc atggcgtccg ctcgcgcggt gcgggcacgg acgcggacgc agtcggcttt
    14521 cgaccgagct cgcgctgctt tgttcgagga gggaatctcc tcgtcccgga tgtatctgga
    14581 ctcggcccgg cccggcgtgg aggacctgat cgacttcatc gtgtccggcg tccgctcgtc
    14641 ctgcacgtac gccggcgccc ggaacctcac cgagttcgca gcccaggcga tcgtcggcgt
    14701 gcagtccgcg tcgggctaca acgagggccg cccgctgcac tccagctggt gacctgaccc
    14761 gcgatcccga tgtaacgcaa cttcgctccc gaatgggact cccgctcccg aattatcggt
    14821 ggcaaacgtc ccattcggga gcgaagttca agccctaggc gtaaccgggc tcactcgtcc
    14881 agcggtcaga tcgaccgggt cagtgctggt attcgtcccg gtagtactgg taggtccagc
    14941 cggtgatcga ccagatgccc atgccgacac cgagcagcgt gatccaccaa ccgaagaccg
    15001 ggccgagcaa catcaccgtc acggtgaggg ctgcccagaa cggccagatg ctgcgcgagg
    15061 ggaagaagcc catctcgccg gcgccctcga tgatctcccc gtccttgcgg tcctcaggac
    15121 gtgcgtccag cttgttggac tggatcgaca ggtagaccgc cagcatggcg cagagtgcga
    15181 aagtcatcag cagtgcgaac gtgccgatga cctcccggct catgaaccag tagatcggca
    15241 ccaccaccag gaagaagatc gacagaaaga cgaagatcca ccgctcggcc ttcactgctc
    15301 aacctccttc tgggtggtct cgtcgatcgc ttcctgcacc ggattgatcg gcgccgcgac
    15361 cgcctcggcc gggtggttca ggtcgaacgc cggcgcctcc gagcgaatcc ggggaagctt
    15421 gttgaagttg aaccgcggcg gcgggctcgt ggtcgcccac tccaacgacc ggccccaacc
    15481 ccacgggtcg tcggtgttca ccagcggcga cttgcggctg atccaaacgt tccagatgaa
    15541 cggcaggaac gagatgccaa gcaggaacgc cccgaccgtg gagacctggt tcaggaaggt
    15601 gaaaccctca ccgggcaggt agtcggcaat tcgacgctgc atgccttcca cacccagcca
    15661 gtgctgcacc aggaacgtca ggtggaagcc gatgaacagc agccagaagt gcagcttgcc
    15721 gatccgctcg tcgagcatgc gtccggtcag cttcggccac cagaagtaga agccggcgaa
    15781 catggcgaac acgaccgtgc cgaaaaggac gtagtggaag tgcgccacca cgaagtaggt
    15841 gtccgacacc gggaagtcca gcggcgggga cgcgagcatg atgccggtca ggccgccgaa
    15901 caggaaagtg gtgaggaagc cgatggacca gaccatcggg gtatcgaagc tgatcgcacc
    15961 accccacatc gtgccgatcc agttgaagaa cttcaccccg gtggggaccg cgatcatgaa
    16021 ggtcatgaac gagaagaaag gcaggttcac cgcaccggtg acgaacatgt ggtgggccca
    16081 aaccgcgatc gacagcgcgc cgatcgccag ggtggccgcg accaggccga cgtagccgaa
    16141 gatcggcttg cggctgaaga ccggcagtac ctcggtgatg atgccgaaga acggcagcgc
    16201 aatgatgtag acctcggggt gcccgaagaa ccagaacagg tgctgccaca ggatcgcgcc
    16261 accggtggcc ggatccagga tgtgcgtgcc gagacgacga tcagcctcca gcaccagcag
    16321 gccggcggcg agcaccggga agcagatcag gatcatgatc gaggtcacca ggatgttcca
    16381 ggtgaagatg ggcatccgga acatggtcat gccgggcgcg cgcagggtca tgatcgtggt
    16441 gatgaagttc accgcgccga gaatcgtgga caggcccatc acgtacaggc ccatcagcca
    16501 caggtcgccg ccgatgccgg gcgagttgat cgcgtccgac agcgggacgt aggcgaacca
    16561 gccgaagctg gctgcgccgc cgggggtgag gaagccggcg cacgaggtca gcgagccgaa
    16621 caggtacagc cagaagctga aggcgttcag ccgtgggaag gcgacgtccg gcgcaccgat
    16681 ctgcagcggc atgatcgcgt tggcgaacgc cacgaacatc ggggtggcga acatcagcag
    16741 catcagcgtg ccgtgcatgg tgaagaactg gttgaacgtc tcgtagtgca tgaattgcag
    16801 tccggggaac gccaattcgg cccggatcgc cagcgccagc aggccggcga acgcgaagta
    16861 gagcagcgag gtgacgaagt acatgttgcc gatcaccttg tgatcggtgg tggtcagcag
    16921 gttcatcgcg aacttgcccc agcgcttgcg ctcaaccagc ggacgcgcct cgacctcgtc
    16981 caacgtcatg cgggcgaccg tcatttgtgc tcctcctccg ccgggacgct gggcaccgtg
    17041 ttcgggtact cgggcgcctt gatctcgccg gtctgaccgg ctgccttgag ccggcccagg
    17101 tacgcctcgt actcctcagc cgttaccacg tggacggtga acagcatcgc cgcgtgatag
    17161 gtgccacaga gctcggcaca cttaccggaa tacgtaccga gcttgtccgg ggtgacgtcg
    17221 aaggagttcg gatgcccagg gatcacgtcc agcttgaagt agaacgcggg gatccagaac
    17281 gagtggatga cgtcggctga gaccaggttg aaccgaacgg cctcgttcac cggcagataa
    17341 aggtcgggga tgcgatcgac cgtcccggcc tcgtggatca cggtgccgac ggccgggttc
    17401 tcggcctcca tgtagttgaa cgtccacgac cacttctggc cgatgacgtt caccgtgcgc
    17461 tgcggttcct tctccttggc cagcacgttg ttctgtgcgt tcacggtgaa gaagaacagc
    17521 actccgatga tcaggaacgg caccagggtg tagagcaact ccagtgggag gttgtagcgg
    17581 gtctgccgcg gtgcctggtc aggatgcttg cggcggtacc gcaccatcac ccagaagatc
    17641 aggccccaaa cgaagacgcc caccaccagg gacgcgatcc aggcacccac ccagaggttg
    17701 ccgatgaacg gcgcgcgatc ggtcgcggcc tcagggagac cgaagcggcc ccattgggcg
    17761 agggtttcag aactgcagcc ggtcagaacg agggccagca ggccggcgaa cccggccccc
    17821 aacaacttct tcgagcgtag ctgtcgaccc acgaggcgcc tttccggtcc gatccctacc
    17881 cctacctgac ttcgatgggc gaaaccctag ccgtccgggt tcactcagtg gaagctatcg
    17941 ccacaagcgc aggagccctg cgcgttcggg ttgtcgatgg tgaagccctg ctgctcgatg
    18001 gtgtcgacga agtcgatcgt ggcaccactg aggtaggggg cgctcatgcg gtcggtgacc
    18061 accgaaacgc cgaagaagtc ggccaccacg tcgccgtcga gttgccgatc gtcgagttgt
    18121 agctggtatc gcagacccga gcagccaccc ggtgcgaccg agatgcgcag cgcgaggcta
    18181 tcgacgtctt cgttggagat caggtccttg accttgctgg ccgcgccctc ggtgagcacg
    18241 atgccgtcgg tcacagtctg ggagtcaacc gtcatatctt cgaatttcct tccgcaacgg
    18301 tttgtcacct gagccaacac tcccagcggg agcgttgttc caagcagtca gttcagttta
    18361 ggtgaggttc gactaagtag atacccagcc ggtcgcgatc cgagccgccg ccgcggtgag
    18421 gtcggcctcg gacggcgtgg cagacgcgag caagtgggcg gcctccaacc ccagcgtgcg
    18481 gagttccctt cgggccaggc cggcgccagt ggtgaataac acgcagggta gctgcgcatc
    18541 ggccgcccag cccgtcaccg cgcttacgac cggcccgcca cgatcgaggg cggacagctc
    18601 cgagcagccg gtgacgacga gatcggcggc ggccagcgtg ccgcccagtc cggcctgccg
    18661 gtggcaaaac tgcgtacccg agacgagctc cccgcccaac gcgagcacgg cgagagcggt
    18721 cgtgccggcc gctcccccgc cgggcgcggt ggccaaacca ggggccagcc ggtcggccca
    18781 cgcgaccagc gccgcgtccg ctcgtagcag gtcggcgacg tccacccggt cggcgaacgc
    18841 gcgcttggcc agcccgccgc cgatgccggt cgccggcagt gccaactcgt ccgctgcgac
    18901 cactccgacc agccgccgac cctccaggac ggagcgcgcc gcggccagca ggccggcacc
    18961 gccgtcctgg gcgttcaggc cggtcaggtc gaggaccacc tcttcttcct cgctcacgtt
    19021 ggccgccacc cactcaccga ggtgggacgt ggtcgcgtcc ggacgccagt tcggggtgcg
    19081 cggctggctg agccccagca ggatgcgtcc gggggcgcgg acgacccagc ggtccgcggt
    19141 tgccgcaggg gcctcgctgc ccaccagcga cgcgaaggcg ttggcgagat ccggcccgcc
    19201 ggccgcgatc ggcaccactg cgacctgcgc attcgcggcg aagcctcgag ccagtgccac
    19261 gccggcggtc atcgaatcca aggcgccgat gcggtcagcg gccaggacca cacggaaggt
    19321 catggccggt cctgcaggaa cgggggttgc tcgaacccgt ccgcgtccgg gccgggctcg
    19381 acctccagcg gctcatcgga cgcgtccgtc atcctgcgca ccgcgcgacg aagctcctcc
    19441 gcctcggcgc gcaacctgcc cacccgggac tcgagcgcga cgcgtccggc ctccaattcg
    19501 gcctcacgct ggttggcgac ggctgggtcc agccgcgtcc gatcgaagac cagccgccgg
    19561 cgagctgcca tcacgtctgc cagctcggcg cgagcgccgt gcaaccccga aagtgcgcgc
    19621 tggtacgcga gctctgcctg ctcgcgcagt tcgttcacgg cacctccggg tgagtttgac
    19681 cggccacgaa tgaccgtagg atgtggcaga catcgtcgca ccaagcgagg tttgaagtgg
    19741 gattgttccg cccgtacgag cgctccgaag ccgaccagcc gaagccggag gccgcaagtt
    19801 cgtccgcgag cagcagcgct gaggccggca agccggccaa gaaggaagtg cccacgccat
    19861 cgcgtcgtga ggcggaggct gcccgccgtg agcggctgaa cccaacgctc acgccgaagg
    19921 aggcgaaggc gcgggagcgg accgccaggg cagctctgcg cgacgagcag ttcgccagga
    19981 ccgagggcac gccgctcaag gtgctgatgc gtgacttcgt cgactcccac cgcggggtgg
    20041 cgcagtactc aatgccgatc ctgatggtca ccctcgcggt gtcgctgctg ctcacgaact
    20101 tcagccagga gttggcggtc ggggtgacca tgttcaccta cgcgatgttc ctgatgatcg
    20161 cgctggacgt gttcctgatg tggcggcggt tcaagaagct cgccgccgag cgcctgcccg
    20221 ggcagccgct caagggaatg ctcggctaca tgatcaaccg ctcgatcaac ctgcgccggc
    20281 tgcgcctgcc tgctccacgg gtgaagccgg gcgacgagat ctgatgggtt actactgggc
    20341 ggcgacccca gagccggcgg acgaggtttc gcgcgacctc ggccttgagg tcgagttcga
    20401 ttcccagggc gaggccgagg cctggctcac cgccagctac gccgaactgg cggacgccgg
    20461 cgtgcacgcc gtcgcgctgt acgagtcgga ccggctggtc tacggaccga tgagcctgga
    20521 cgcctgagcc tggcccacca cgatccctga gcctggcgaa ggtccgtcca gcaacgggct
    20581 tcgacaagct cagccaccgt ttgctgctct gggttagcgc aagcgtccct cgatgaccgt
    20641 cgcggtctgc cggagccgct ccagtacgcg ttcgggatcg ccggccagcg gcgtccgcca
    20701 ggtgaccgcg accgccgcca gattggacgc cgaccgcagc ccgaccgcga cagacgcgaa
    20761 gcccgcggtc acgtccgaat cctcctcggc gtagccccgg cgacgggtga ctgcgaggag
    20821 cgtccggagc tcggcaagcg tccgtgggcc gctttcggtg cgccgcacca gcgcagcacc
    20881 ggacgggtag agcgcccgca cgtgcgccgg cggcagtccc gccaggatcg cgcgtcccga
    20941 agcggtgagg tgcgcaggca accggacgcc gacgtcagtg accagccggt ccgctccggt
    21001 tgcacgctcc tccaccaggt agacgacgtc tgcgcccagc agcaccgcga ggtgtgcgga
    21061 gaggccgagc gagtcgacca gacgggcgat cggcacccgg gccagccggg tcagcggtgc
    21121 ctgccgggtg tagccctggc ccacctccca ggcggcgaca ccgactcccc agagctggtc
    21181 ttcggggtag tgcaccacga acgactcggc cgccatcgcg gtgagcaggt ggtacatgct
    21241 cgacctgggc aggccgagat cgtgcgcgat ccgcgccgcc ggaaccggcg tggtgcgctc
    21301 ggagaggtag cgcaggacgc gaagcgccgc cgaggctgca ggcactggac gggtcattgc
    21361 ccaagtctcg catccgagac aactggctcc ggtcgagggt agcgaaagcg ggcgtggctg
    21421 gcttgaatct gacccatgga gatcgtgttg ggagcggttc cgctcacccc ggccaacgtg
    21481 gtggcggtcg ctcgcgcagg cgcgcgggtg cggctgtcca gcgaagccac ggcagcgatc
    21541 gacgcgtcga gggagcacgt ggcggcactg gccgcgaccg ccgaaggcgt ctacggggtt
    21601 tccaccggct tcggggcgct ggccacgcgc agcatcccgg cgcccgagcg ggcgcagttg
    21661 cagcgcagcc tgattcggtc acacgcggcc ggcaacggtc cgctggtcga gcgcgaagtg
    21721 gtacgggcga tgatgctgct acgcatcgcg acgctggcca agggcttcag cggggtacgg
    21781 ctggcgaccg tcgaggcgta cctcggattg ctgaactcag ggctggtgcc ggcggtgcac
    21841 gagtacggca gcctcggctg ctcaggtgat ctggctcccc tggcgcactg tgcgctcgtg
    21901 ctgatgggtg agggtgaggt gctggacggt tcggtggttc gtccagcggc cgccgcgttg
    21961 gccgagcatg ggctggcccc gatcgagctt gccgagaagg aaggcctggc gctgatcaac
    22021 ggcaccgacg gcatgctcgg gcagctggtg ctggcgctgg ccgacctcgc gctgctgctg
    22081 gacacggccg atctggctgc cgcgctgagt atcgagggcc aactcggcac cgaccgcacc
    22141 ttcgccgccg acctgatggc gctgcgtccg cagttggggc agtcactctc ggccgccaac
    22201 ttgcgggcgt tcctggccgg ttcgccgatc ctggccagtc accggtacgg ggacggggtc
    22261 gtgcaggacg cctactcgct gcgctgcacg ccgcaggtgc acggctcact gcgggacacc
    22321 gccgatttcg ccagccggat cgccgagcgc gagctggctg cggccatcga caacccgtcc
    22381 gtgctggcgg acggtcgggt cgagtcacac ggcaacttcc acggggcgcc gctcgcgcac
    22441 gtgctcgatt tcctggccat cggtgtggcg gacgtggcct cgatcagcga acgtcgcacc
    22501 gaccgcttcc tggacgtgca ccgcaatcgc ggactgccgc cgttcctcgc cgacgacccc
    22561 gggatcgatt cgggcctgat gatcgcgcag tacacccagg ccggaatcgt gtcgacgctg
    22621 aagcggctgg ccgtgccggc gagcgtggac tcgatcccgt ccagcgccct gcaggaggac
    22681 cacgtctcaa tgggctgggc agcggcgcgc aagctgcgga cggctctgga cgggctcgcg
    22741 cgggtgctcg ccatcgagat cctgactgct tcgcggggcg tggagctgcg ggcgccgctg
    22801 cgtccggggg tggccacggc ggcggctatc tccgtcctgc gggaaacgat ccccggcccc
    22861 ggcccggatc gcttcctcgc cccggagatc gaggcggcct acgtgctcgt ccgctccggc
    22921 gcattggttg ccgccgccca ccaggccacc gacgcggatc ccggccttcg ccgggatgac
    22981 gtggtggaga agaccgtcat cccgggcttg ccccgggatc tcccgtccgc acccgaggag
    23041 aaccgatgaa ccacgaaccc gtccgcgcgc cgcggggaac cgccctcagc gcgaagtcct
    23101 ggcagaccga agctcctttg cgaatgctga tgaacaacct cgaccccgag gtcgccgagc
    23161 ggcccgagga cctggtggtt tacggcggca cgggccgcgc ggcccgctcg tgggaggcgt
    23221 tcgacgccat cgtcgccagc ctgcgcgacc tcgccgacga cgaaaccctc ctggtggcgt
    23281 ccggcaagcc ggtcggcatc ttccggacgc acgaatgggc gccacgcgtc ctgatcgcca
    23341 actcgaacct ggtgggcgac tgggccacct ggccggaatt ccgcaagctg gaggccgccg
    23401 gcctaaccat gtacggccag atgaccgccg gctcatggat ctacatcggc acccagggca
    23461 tcctgcaggg cacctttgag actttcggcg cggtcgcgcg caagcggttc ggcggcacgc
    23521 tcgccggcac gctcaccctc accgccggct gcggcggcat gggcggagcg cagccgctgg
    23581 ccgtgaccat gaacgagggc gcggtgctga tcgtcgacgt ggacgcgtcc cgcctcgccc
    23641 ggcgggtcga gcacggctac ctcgacgaat tcacgtccga ctacgccgaa gccgtggcca
    23701 aggtgctggc cgcgaaggcc gagcggcgag cgctttcggt gggcctggtc ggcaacgccg
    23761 ccgaagtgtt ccctcgccta ctcgctgagg gggtcgcggt ggacgtggtc accgaccaga
    23821 cgtcggcgca cgacccgttg gcctacctgc cagtaggggt ttcggtcgac gagtggcgtg
    23881 aactcgcggc gaccgatccg gagggcttca ccgagcgcgc gcgtgcgtcc atggctgccc
    23941 acgtcgaagc gatgctcggc ttccaggacc gcggcgccga ggtcttcgat tacgggaact
    24001 cgctacgcgc ggaggctcag ttgggcggct gcgagcgggc cttcgacttc cccggcttcg
    24061 tgccggcgta catccgtccg ctgttctgcg aagggaaggg accgttccgc tgggccgcgc
    24121 tgtccggcga cccggccgac atcgcggtca cggacgcggc gatcctcgac ctcttccccg
    24181 acgatgccca cctgcgccgc tggctgaccg ccgcgtccga gaaggtgcac ttcgagggcc
    24241 tgcccgcccg gatctgctgg ctcggctacg gcgaacggca cctggccggg ctcaagttca
    24301 acgagttggt tgcttctggg gcggtctcgg ccccgatcgt gatcggacgc gaccacctcg
    24361 attcgggctc agtcgcttcc ccctaccgcg agaccgaggc gatggccgac ggctcggacg
    24421 cgatcgccga ctggccgctg ctgaacgccc tgctgaacac ggcatccggc gccacctggg
    24481 tgtcgatcca ccacggcggg ggcgtgggga tcggccgttc gatccatgcc gggcaggtag
    24541 tggtggccga cggcaccgac ctggccgcgc agaagatagc gcgggtgctg acgaacgacc
    24601 ccggcaccgg cgtgatgcgc cacgttgatg ccgggtacga acgtgcccgc gaggtcgcgc
    24661 gcgagcgcgg cgtccggatt ccgatgctct gatgtccgga gcaaacgaac tgctggccga
    24721 ggtcgccggc atcggccggg agtccggcgg tggctacacc cggctgtcgt gggacgcggc
    24781 cacgatggag ttgcgcgagt ggttcgccgg ccaggcggac gcgctcgggc tcgatctggt
    24841 cgtagacggc aacggcaacc aggtggcctg gtggggctcg ggaactggtg ccctgctgct
    24901 gggctcgcat ctggattcgg tgcccaacgg tggtccgctg gacgggccgc tgggggtcac
    24961 gtcggcgctc gctgcggtgg cggcattgca gcgggagggc tggcggcctg cgcgtccgat
    25021 cgcggtggcg aacttcaccg acgaggaggg cgggcgcttc ggggtggcct gcctggggtc
    25081 gcgactggtc accggagcga tcgcgaccga aaccgcgttg gggctgaggg acgcggacgg
    25141 caatacgctc gccgaggtga tggctgcccg cggtttcgca cccgaggctg cgggcgttgc
    25201 cgactggcta gaccaggtgt cgtcatacat tgaactacac gtggagcagg gtcgctggct
    25261 tgcgccagct gcggccccgg tcggcgtggc caccgctatc tggccgcacg gccgctatcg
    25321 cttcgacttc tgcggacagg gcaaccatgc cggcgcgacc gcgatggcgg accggcacga
    25381 cccgatgctg gcctatgccg ccacggtgct ggccgcccgc gagctggctt cggcgaccga
    25441 ccagcgggcc acgctcggcc gggtgcggat cgaacccaac agcacgaact cgatcccttc
    25501 gcgggtcacc gcttggctgg acgctcgggc ttcgtccgaa gcggggctgg aggcactggt
    25561 cgcagcaatc gtcagcaagg cccaactcgc cgccgaagtt gatggcacca gcgtcgaggt
    25621 gcgcgccgaa tccgtcagcg gcgcggtcga gttcgaccac gcgcttcggc atcggctcgc
    25681 cgcgctgctc gatgcccccg tgctgcccac cggagcgggc cacgacgccg gcatcctggc
    25741 ggcgaagctg cccacggcga tgctgttcgt gcggaacccg accggggtgt cgcacgcgcc
    25801 cgaggagttc gcgacccccg aagacatcga cgccggggtc gccgcgctca tccaggtcgt
    25861 gcgggaggtc gggtccgagg cagcacgatg agcggcgcca cctatcccgc cttcgcgaac
    25921 tgccattcgc acgcttttca tcgcgccctg cggggtcgag ccggtggcag cgacttctgg
    25981 ggttggcgcg agggcatgta ccgggtggcg caggtgctcg atcccgacct ctactatcgg
    26041 ctggcccgcg cgacctacgc cgagatggcc ctggccggga tcggggcggt gggcgagttc
    26101 cactacctgc accacgaccg cggtggcgtc cgctatgccg agccgaacgc gatggccaat
    26161 gcggtgatcg ccgctgcctg ggaggttggc ttgcggatct gcctgttgga cacctgctac
    26221 ctgcgcgtgg gcttcgacga cgccgaaccg gcagctgcgc agctgcggtt cttccacgcc
    26281 gatgccgccg agtgggctgc gggtgcgact gatctggccg agaagtacgc cgacaatccc
    26341 gccgtagtga tcggggcagc gatccattcg gtgcgtgccg ttgacgaagc ctcgctgccg
    26401 atcgtcgcca gcgcgctccc ctttgcaccg ctacatgtgc acgtttccga acaaccggcg
    26461 gagaacgccg cctgtatggc agccaccggt cgcaaccccg tcgagctgtt ggccgacgcc
    26521 ggcgtctgga cgccgcgcac caccgcggta cacgccaccc acctgaccga tgccgacatc
    26581 gccaccctcg gcgcggccgg cggcttcgtc tgcttctgcc ccaccaccga ggccgaactg
    26641 gcggacggga tcggccccag cattgctctg cgcgcagccg gcgcccggat caccctcggc
    26701 tcggattcca acaccgtgat cgacttgttc gccgaggccc gcgggctcgc gctgcacgaa
    26761 cgcttggcca gtggcttccg cgatggctgg tcggcggcaa agctctggca ggcagcgacc
    26821 acggacggcg ccgcgtcgct gggcttcgag ggtctgggcg gcttcgaact caccgaatca
    26881 gtgcggaccg ccggcgcggg cgagccgatc tgggctgcct ccgccgcgga cgtgaagccg
    26941 gcgcccgacc tggatccggg ttgggtgacc gccgaactgc aggcagccat cgacgaggtc
    27001 tggagtcgga catgagcgca gcttcgctag ccatcaccag tatcggcgaa ctgaccacct
    27061 gggacgacga gcggcccatc cgccacgacg cgggcctggt gatcgaagcg ggccgggtcg
    27121 tctgggtggg tgcgtccggc gacaccccgt ccgccgatcg caggatcgac gcccagggtg
    27181 gggcggtgat cccaggcttc gtcgactcgc acacccatct ggtcttcgcc ggcgaccggg
    27241 ccgaggagtt cgcggcccgc atggccggca aaccgtatgc agccggcggc atccgcacca
    27301 cggtagcggc gacgcggtca gccaccgagc ccgaacttga gcgcctcgcc cggcaccggc
    27361 tagccgagct ccgcgcccag ggcaccacca cggtcgagat caagagcggc tacggcctcg
    27421 acgtggccac cgaggagcgg ctgctgcggg tcgcgtccgc gttgaccgag gagaccacct
    27481 ttctgggcgc gcacgtcgtg ccgtccggct gggaagcgaa cgcctacgtc gagctggtca
    27541 ccggcgagat gctggcccgt tgcgccccgt tcgccaagtg ggtggacgtg ttctgcgagc
    27601 ggggagccat cgacgccgat caggcgcggc tggtgttgtc ggccggcatc gccgccgggc
    27661 tgcagccgcg ggtgcatgcc aaccagctcg gccccggtcc gggcgtgcag ttggcctgcg
    27721 aactgggcgc cgcggcggcc gatcactgca cctacctgag cgattcggac gtgtcagcgc
    27781 tggctgagac cggagtggtc gccgggctgc tgccgggagt ggagttctcg actcggcatc
    27841 cctatccgga cgcccggcgc ctactggacg ccggggtgcg ggtggcgatc gccaccgact
    27901 gcaatccggg cacgtcctac acctcgtcga tgccgttctg catcgcgctg gctgtgcgcg
    27961 aaatggggat gactccgacc gaggcgctgc acgcggccac ccagggcggc gccgggtcgc
    28021 tgcgccgcga ggatgtcggc cacctgcggc tgggtgcgcg ggccgatctc gcgttcctgg
    28081 acgccaccag ccacgtgcac ctcgcctacc gcccgggcgt gcccctggtg aagcgcgtgc
    28141 tggtcggcgg cgacgcttgg cccgggctta gccctcgcgg acgccgggct ccacgaacgg
    28201 gggcttgacc acctcgaagg gctccagccg gtcccggacg agcacgtcga cttggtcacc
    28261 gaccgcgacc gaacggtcca ggagcgccaa agcgatgccc gcgcctagcg tcggcgagaa
    28321 cgtccccgac gtcacctcac caacgcccac gccgccggcc tgcaccgcca tgtgcggccg
    28381 cggaattccc cgtccgagcg ccttcaggcc ccgcagcagc ggcacggcct tcgcctcgcg
    28441 ctgggcgcgc aacgcatccg caccccagaa ggtgggcttg gtccaaccca ccgcccagtt
    28501 caggttcgcc atcactgggg agatctccgg gctcaggtcg tgaccgtgca gcgggtagcc
    28561 catctcggtg cgcagggtgt cccgcgcacc cagtccacag gtgaggatgt cgtactcccg
    28621 gccggcggcc agcactgcgt cccacacctg caccgccgcg gacgcgggca cgatcagctc
    28681 gtagccgcgc tcccccgtgt agccggtgcg gcagacgatc aggtcgacac ccccgtagga
    28741 acccatcagg aaggtcaggt agtcgtgccc gaccggcagc ccgagcgccg caagggtctc
    28801 gtccgaattc gtgccctgca ctgcgatcac ggcgtggtcg cgatgctcgt tgcgcacctc
    28861 gatgccggcc ggcgccgccg cgctcagcag cccgtaaacc gttgcgttgt tggcggcgtt
    28921 gggcaccagg aagacctcgt ccggcgagat caggtaggtg aagatgtcgt cgatcgcccc
    28981 gccggcgtcg ttgcaggcca gcgtgtactg cgcctgaccc ggctgaatgc ggccgagatc
    29041 gttggtgagg cagctattca cgaattccgc ggcgcccgtc ccgagcacgc gcaccttgcc
    29101 gagatggctg acgtcgaaca cgccgaccgc gttccggacg gcccgatgct cggccaccac
    29161 cccggagtac tgcagcggca tcagccagcc gccgaactcg gagaatttgg cgcccagcgc
    29221 ctcgtggcgt tcgtgcagcg gggacttcag tggatcagtc atcttctgcc ctcctggcga
    29281 tcagggtagt cacatcgagc ggcgaagcac cgcagcgaac gcttccggcg tcatcggcac
    29341 ggggttgccc ttgctgctgg acgcgttcgt ggccgccacg gcgatcccgt ccacgtcggc
    29401 gtccgccaag cccaaagcgg ccagcccggg aacgcccagt tcggcggcgg tatcggtgaa
    29461 ccagccgagg aaggccgcgg cctcgcggcg gccagtgagc agttcgccca cttcggcgta
    29521 acgcgccagc ccctgccgat catcggacgc cttcagcacc gccaggttgt gctcgctgac
    29581 cgccaccatg acggacgcgg tgatcgcacc atgcggcgcg ccgaggaacc cacccagcgg
    29641 gccggccagg ccgtgcgccg cacccaactt gccgttggca agcgccaagc cggagagcag
    29701 cgagcaaacg ctcacgtcgg tgcgcgcgtc caggtcgtcg gggtggtggt acgcgcgctg
    29761 cagtccggac gcggcccgcc ggatgccctc tcggcagaac ccgtcggtga gcggcgtggc
    29821 gaacggcgac acgaacggtt cgatgcactg cacgagggcg tccagaccgg cgtgggcggt
    29881 ggtcgccggc gggcaggtga gggtcagttc gggatcgacg atcgccaccc gcggcagcat
    29941 ggacgtcgac cgcaggctca ccttcacccg atgctccgtg gacgtgacca cgccgttggc
    30001 ggtgacctcc gagccagtgc cggacgtggt cggcagcgcg accaccggca gcgaggcgac
    30061 gcgcagcggc cgtcccgcac cgaccacctc ggcgtagtcg agcgggtcgc cgccgttggt
    30121 gaccagggcg gcgatcacct tggccgcgtc cagtaccgca ccgccgccga gaccgatcac
    30181 cacatcggcg ccgtgctcgc gcgcagcctt ggcgccggcc cggacgacgt ccatggtcgg
    30241 ctccccggct accgcgaaag tggcgtagtc agccagcgtt tcgtagaccg agaggtagcg
    30301 ggacgcgtcc gagccggtgc agaggaacgg ccgccggccg agactggcga ccacgtccaa
    30361 cagttcggag ctgcggccgg cgccgaaggc gatgcgtccg gcggtggcga agttgaagga
    30421 gatcacggac tcatcctgcc cccatcgccc gaccatcccg caagctggac atctgccgcc
    30481 gacatcggcg atttcggtcc accttgcgga g
//
LOCUS       DAOPRQ010000028        29038 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_1271_length_29038_cov_13.054722, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000028 DAOPRQ010000000
VERSION     DAOPRQ010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 29038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29038
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1271_length_29038_cov_13.054722"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2781)
                     /locus_tag="PKJ61_05330"
     CDS             complement(<1..2781)
                     /locus_tag="PKJ61_05330"
                     /inference="COORDINATES: protein motif:HMM:NF024209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HOC13277.1"
                     /translation="MSRLRGLAVAVAASVTFLALPPVTASAATFNDTMPDANYRACVN
                     TKLGLAADADPSAEQLATITELSCTGKGITDVTGTAALTNLTKLFLGGNSITNIAGLA
                     SSTKIFSLGLENNQLSNIAPLSALSKLSAVTLAGNKLRDLSVLGTLPAYSSGSGTRYG
                     QQAVAAAAATGVAAIVPTIVNAAGSLVVPTTPAGATVSGTTVTYAGPGSYEWTFRDPD
                     DFYFNGKVTVVVTDTADTVPDAALRACLNTRIGNADTTVQPTVAQLAALTGALSCANK
                     GVQDLTGLNLATGLTGLTLNGNSISDVSPISALVNVTTANLSLNEITSIAGLGTLPKL
                     GTIQLQQNLASTKAKLQTLAGVNKLTALTAITANYTAIRSLAPLAGNTSLKNLTATNN
                     QLSDLTPLATAGSQLANVDLSSNQISDLSPVKSKSYTKLAVTTQTIAAADATATQETA
                     APAVTKKDGSALVATPPAGVTTSNSKVTYASAGSYNWTFSFTDSLQGVTFGGTITQVV
                     KDAPPPVVEVAVPDASFKACLAGLLKQDTSAAITADQLEALTSVTCIDQGIVELTGAE
                     HLVNATELVLSTNAVSNVAPLAGLTKLTKLYLPGNAISDPSALASLTGLDELLLSYNP
                     ISSISALAPLVNLTNLEVTQKFGHTGADLTSLDGVQGMTKLTRLVANNSSLTSLEPVT
                     GLKSLKKLYVSNNQITDLSPVQGLTELTGFGAFSNRISDVSPLAGLTKLTDVDLEYNQ
                     IGDLSALSGLTNLGYLGLKAQGQVVTAKSVPAQFTTSAPKPRTNTGTAATLTAPDGIT
                     VTDQKVRYPAEGSYTWTFSAKDAESSTEFFAGTITQPVTAPVSGAADVPDANLRSCLA
                     EAVGLSASDVPTEAELGTVTSADCAAGGITDLTGAELLTAATALNLSGNPLGNIAPLE
                     KLSKLSELNLAST"
     regulatory      complement(2884..2937)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(3085..3972)
                     /locus_tag="PKJ61_05335"
     CDS             complement(3085..3972)
                     /locus_tag="PKJ61_05335"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HOC13278.1"
                     /translation="MPDFTTWIPVNSLTSIGVVIVVAIVARFLLVRLVRGITNRALAK
                     GKKRRERELSVAEKLLNTVTLANQERYEQRTATLGSVIASVIGVVVWTIATLTILNIL
                     GIPMGPLLTSAGVGGVALAFGAQSLVKDFLSGIFMIMEDQYGVGDLVNTGEVTGTVEE
                     VGLRTTRLRDGTGTVWYVRNGEILRIGNQSQGWSTAIVDVPVAYDEDATKVIGILEEV
                     AAEIAEDPDYAEVLLDKPAVAGVNAVTATTMSIRVTAKTATNQHWGVQRSLLERSLNA
                     LAKAGVRSPTTMILPPNAG"
     gene            4167..5360
                     /locus_tag="PKJ61_05340"
     CDS             4167..5360
                     /locus_tag="PKJ61_05340"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012917980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HOC13279.1"
                     /translation="MQLGIFSVGDVTTDPTTGRTPTEHERILAAVAIAKKAEEIGLDV
                     FATGEHHNPPFHPSSPTTLLGFIAAQTSKLILSTATTLITTNDPVKIAEDYSVLQHLA
                     EGRVDLMMGRGNTGPVYPWFGRDIRDGISLAIENYALLRKLWRDEVVNWEGKFRTPLQ
                     GFTLAPQPLDGVPPFVWHGSIRSPEIAEQAAYYGDGFFHNNIFWNLSHTKRMVNLYRR
                     RFEHHGHGPADTAIVGLGGQFFMRRNSQDAINEFRPYFDVAPVYGYGPSLEEFMTETP
                     LIVGSPQQVLERTLSFTDAVGHYQRQLFLIDHAGLPLKTVLEQLDLLGEILPELRKGY
                     AEGRPAHIPDAPTHSSLVAARDARSLGEAADAVSTGATARGFVDEVTGRSPYDTATTS
                     QGVER"
     gene            5357..5953
                     /locus_tag="PKJ61_05345"
     CDS             5357..5953
                     /locus_tag="PKJ61_05345"
                     /inference="COORDINATES: protein motif:HMM:TIGR04037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOC13280.1"
                     /translation="MTSIVVVSAGTGTPSSSRLLGERISDAVVVALAEANTQAEVTHI
                     ELRTIAVELAQHLTTRVPGANLRRALAAVRAADGLVVVSPVMNGSYSGLFKLFFDALE
                     EDALRGHPVLLAASGGTARHSLMIDQAMLPLFFYLKALVAPLSVFAATKDWGTAGGKL
                     ERRIASAAQAFATLVLSADPIETTDELTVTDFSELLGG"
     gene            complement(5954..6817)
                     /locus_tag="PKJ61_05350"
     CDS             complement(5954..6817)
                     /locus_tag="PKJ61_05350"
                     /inference="COORDINATES: protein motif:HMM:NF025215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation family protein"
                     /protein_id="HOC13281.1"
                     /translation="MQLRRAAVVLALGLALAGCTSTTAPSPSTPTSAPASSAGPSTPA
                     ESVTPEVPAATVSVPQVGECTGPVDLSGASIASLTAIPCAQPHYYEVQARVPISGDAY
                     PGAEALGEQAMTKCSDGFADYVGAEPGYSRYSSAYVVPDEVAWATPANRVITCLVGSA
                     DGGLTGSAKGDFLVFPKKGQCTGPQDVPALELELIDCASEHHYEVFAASNVKGKKAPT
                     DAEVEKLFASVCLAGFQKFVGVAAGQSKYEVTYFIASAELWEKVADHRIVCSAGSPKG
                     GVKGSLKGVKK"
     gene            6893..8134
                     /locus_tag="PKJ61_05355"
     CDS             6893..8134
                     /locus_tag="PKJ61_05355"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOC13282.1"
                     /translation="MRGNDVWFPPGATRHDWVLDAALAAGLGLLTIPPYGVRGFAEAF
                     TTGQFASAVLMIGALVFRRHSPLLALAGVSVAALMQLVLSSTPMATLVAVPVVAYSVA
                     RWIPGRQARIALAIGSIGAVLGPVRWIVEDPYYLSLRDLIWVVLAWFVCMGLVVTPYA
                     VGRRVLESTQTHHHRVLAAEERYRTTLAERDQEARLAESRARTQIARELHDIVAHSLS
                     VMIVQAEGGRAMATKKPEAATQALTTIAETGREALSEMRRILGVLRSEPEMLPQADFA
                     PSPRLTDIPDLVARTSDRVRLTVQGQPPRVSPALELTVYRVVQEALTNFLKHAGPQAT
                     AAVTITYGMREINIDVIDNGVATPSAVPQNPGHGLRGMSERVAAMGGRLLTRARPTGG
                     FQVTAVLPLPLYTTAQEDDQR"
     gene            8131..8787
                     /locus_tag="PKJ61_05360"
     CDS             8131..8787
                     /locus_tag="PKJ61_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012919972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOC13283.1"
                     /translation="MTIRVFLADDQELVRSGFRMLIEAEDDLQVVGEAPDGAGTVVGV
                     LRHPCDVVLMDIRMPVMDGVEATRRIVASGVPTRVLVLTTFDLDEYVFSALKAGASGF
                     MLKDARPSDLLNAIRTVAAGEAVMAPSATRRLLDHVVPRLPNNPEGPNPRLDVLTDRE
                     REVLVEIAAGATNAEIAAKLYMAEGTVKTHITRLLSKLQCRDRVGLVLFAYETGLVRH
                     "
     gene            complement(8926..9948)
                     /locus_tag="PKJ61_05365"
     CDS             complement(8926..9948)
                     /locus_tag="PKJ61_05365"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HOC13284.1"
                     /translation="MRLLFLGAGAIGTYVGGSLAAAGHHVTFSEQPEAARIIAEQGLR
                     LTRDNRTTSVRDFAIFDSADAALAAGPYDAMVFALKSFDTESALTGLQATGAELPPVL
                     SLQNGVDNEPKLVAALGADRVITGTVTTAVSKPGIGQVIEEKRRGIGIALDHPLGEAL
                     VSALNDADLNGVGYPQPGPMKWSKLLTNLTGNATSAILDEPVGALFADRRTYAIEVAV
                     LRECLAVMAALGYRPVDLPGTPVRALALAAQRLPRFIAQPALTRALGGGRGDKMPSFH
                     IDLHGGRGQTEVQFLNGAVVRHGDELGVPTPVNRVLTETLEDLSAGRQSLDTYRHNPD
                     ALLKLL"
     gene            complement(10012..10575)
                     /locus_tag="PKJ61_05370"
     CDS             complement(10012..10575)
                     /locus_tag="PKJ61_05370"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HOC13285.1"
                     /translation="MPDFTVKPTLAGELVTLRPAAAADAAILHQAMADAEVSRLTGSV
                     HSSTATDEEQWTVADLEEIYGRWATAPDRIVWVVIENSTGKIVGESVLNDLDEGNLSC
                     GFRIWITGARDRGLGTEAVRLSVGHAFASGLNRVELEVYAFNPRARHVYEKVGFQLEG
                     TKRQALRFDDEWVDCHVMGLLAQDWTG"
     gene            complement(10568..10741)
                     /locus_tag="PKJ61_05375"
     CDS             complement(10568..10741)
                     /locus_tag="PKJ61_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="HOC13286.1"
                     /translation="MTSNTAARNQRRTLVYSVRLSPSESNAIQKIADARHLPASTLVR
                     SWILDRLDQEQGA"
     gene            complement(10767..11027)
                     /locus_tag="PKJ61_05380"
     CDS             complement(10767..11027)
                     /locus_tag="PKJ61_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13287.1"
                     /translation="MALRFASKSDEYLACCGVAAVGRLILEAPDDLSLVELLTARAAD
                     ELWRVREAAAIAGQLIGDQDAVRLRTLVADWRQRPTQTDLFN"
     gene            complement(11232..12476)
                     /locus_tag="PKJ61_05385"
     CDS             complement(11232..12476)
                     /locus_tag="PKJ61_05385"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOC13288.1"
                     /translation="MRSYREILAIPGALKFSAAGVLARSGGAMMGLGIVLMVSALYGS
                     YGLAGALAAANGVAWAIGTAVLSNLVDRYGQRRVMYPAVVVSATMLMVVVVFAVLQLP
                     AWTLFPPVIISGATGGAPGALVRARWNHATSDPGQLHTAYSLESTLDELTFVIGPVAA
                     TALSVGVHPAAGLVAPIILSLVGAKLFYSQRETEPPVMPRPASQQQTPLWRRLILMAP
                     GVGAVVAVNLLIGCVFGAIDVSVVAAATSWDVRAASGVVLAVFSLASGLAGFYYGSRT
                     WSSPLPGRFVVGVLALFASTLGLLLANSILLLSAVGFLVGVTVAPTLINGNTLIGRLV
                     PRERLTEGLSWMGTGIGIGASIGSSVAGQLIDSSGYHASLVSVAAFALVAAAIALASL
                     RTLRRAAVNTDPEYAEDADAVH"
     gene            12611..13471
                     /locus_tag="PKJ61_05390"
     CDS             12611..13471
                     /locus_tag="PKJ61_05390"
                     /inference="COORDINATES: protein motif:HMM:NF014600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HOC13289.1"
                     /translation="MARGRRLVAAVAVVLLSWASPGLAEVADARPLGLISEISPPAET
                     PSPEPPPAPSPEPTPEPTPSPTPEPTPEPTPTPEPTPTLTPTPTPEPDCRGALDDRKS
                     AKAKRAPFVVCDTVVISKKHRVTSAYKPSLSRVQVPTYGLSGVYLQAKAGTQLKAMFA
                     AAKKAGYTLVVRSAYRSYATQKRIYRPGAKLTAPAGASEHQSGLAVDLAWKRGRIVRG
                     YTFGSSKAGAWVRAHAADYGFILRYPKGKQKITGIPYEPWHFRYVGESVANGVRRTST
                     KTLEQYLRID"
     gene            complement(13479..13739)
                     /locus_tag="PKJ61_05395"
     CDS             complement(13479..13739)
                     /locus_tag="PKJ61_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13290.1"
                     /translation="MGMARFRKRYRGRNFGANFFGIGGPDEDSIAGRVVYVDVARAFN
                     GHGLCDTGLPWVISGLRPAAAGPPNLLGQAAYAAVLYGAGVR"
     gene            14462..14881
                     /locus_tag="PKJ61_05400"
     CDS             14462..14881
                     /locus_tag="PKJ61_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015583456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5318 family protein"
                     /protein_id="HOC13291.1"
                     /translation="MWSKREVVDYALQRRNSLQALRRPQRSLESLSICDADPLLIRAA
                     THHGEESRVPCPVCEAERTTTLNYVFGDQLGQYSGRIKSTVELDEMQSDYGEFKVRVV
                     EVCVRCGWNFLITSYLLGDGVTRRPPRRQPTVEDIYG"
     gene            14874..17075
                     /locus_tag="PKJ61_05405"
     CDS             14874..17075
                     /locus_tag="PKJ61_05405"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HOC13292.1"
                     /translation="MADPKRPAAAPHSQGKNKKKRIWLRVLAWTMSILLVLGLAGVGV
                     IAYAYSTIEVPDPNSDFQTNTSFVYYADGKSKIGSFQVQNRQSITYEEMPQYVKDAVV
                     AAENRTFWEDPGISVTGLFRAALGLVGISTGGTESGGGSTITQQYIKIMYLTQEKSFT
                     RKAKEILLAAKIGQELTKEEILERYLNTVYFGRGAYGIQAAAQAYFNKSAAKLTLAES
                     VALAAILNSPGNLDPANGDAEAADLLERYQYTLNGMVELGMITEAQKAEIYRTLPEFP
                     EPKTNSQMGGTKGFLLSLVQKELLRAGFSEEEISGRGLKIVTTFDKAAQAAAVDAAKE
                     TALQAAGGDKKAAAKLHPALASIDNATGGVVALYGGPDYVKSQWNWATNPRPTGSTFK
                     PYALAAALRNEWTLNDKLNGNTFTPDGDSRPVRNAGGANYGRITLQQATTKSVNTAYT
                     DLVSQIENGPQEVVTAATDAGLPESTDWDPSNRIPLGRTEVSPLNQASAYSTFANQGV
                     HRTPHVVAEVFDVQGRSIYAADTNGSQTIETDVAIDVTYALTKVTQDGTGYRASQLGY
                     EVAGKTGTYYVADGGRSETRAVWFVGFTRQLTTAVMYVKGDQGTTDLGSGWFGSGWPL
                     TTWLAYMRTAMDGKEHEGFDPPTVRVSTQTPTAKPSKTAEPATENPLPTETVPTEPVP
                     TVTVPGEPTVPQPTQPTQPTETASVPSGQPTVTEGAQAAVDPGAQTVAAGP"
     gene            17085..18431
                     /locus_tag="PKJ61_05410"
     CDS             17085..18431
                     /locus_tag="PKJ61_05410"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HOC13293.1"
                     /translation="MTQTPAALSNVLGGALGKHVRPGGVWFNPAPWTYLALTLSWLVL
                     ALRQLPCRNSSDMFPNMCYTDITVLYYWRGLQDGKIPYLDSDVEYPVLTGAIMELGRR
                     LVVLFGGRSEPGLSSTEISHAAGLFFGINIVLLFGLFVVLVWAHLQFHRPWDALMIAI
                     APAIWTSALINWDGLVLALTALAMLAWKRKSPVWTGVWLGLGIAAKLYPVLLLVPLAV
                     LCLRTSRWRPFFITLGATVASWLAVNLPVYLATPSGWLNFWTFNVDRGGDLGSIWYVL
                     GLAGYQVDSVSKLVAAIMVAGTVVICGLLLLAPRRPRFAQGVFLIVALFLMVNKVYSP
                     QYVLWLLPLLVLARPRWLDWAVFSIAEGFYFAAIWAHLDGRSASGDGHDRMYWLSVLL
                     RIAVQFWLCVRVALDMVRPERDVVRAGSLDDPDGGIFDHASDAPWLVRLNQRLAAR"
     assembly_gap    18511..18610
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            18711..19802
                     /locus_tag="PKJ61_05415"
     CDS             18711..19802
                     /locus_tag="PKJ61_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015069215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000009 - alpha-1,6-mannosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0004376 - glycolipid mannosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006506 - GPI anchor biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyltransferase family protein"
                     /protein_id="HOC13294.1"
                     /translation="MTTSVHRSADARLVVQTWFATRLLMALVGVWLMLTEGRKFSDLV
                     GNWDVQHFLAIAADGYVARHNIAFFPGWPLLLRLTSTTGLPIMIAGTLLALLASGFAA
                     AALYRMAGAPAAIAWLLAPTAVFTMVPYTESVFCAAAFWAWERATAKQWGAAAVLAAV
                     AAAVRVSGVFLIVALAVLAITSAGSSRDKGKRLLWLLFPLCVVVAYLAYLYSMTGNWA
                     AWYEAQAAGWSRGFTWPWDSLRHTFEAFAPGAYADHPEWAWVFRAEVVSMAVGVVVTL
                     GSLVRRRWGEATWVGLQVAAFSVSYWFMSVNRAVLLWFPLWTWLGELGGSRRKLPTWR
                     AILIGLLVAVALVTQAIWAWLFFTGRWAA"
     gene            complement(19863..20252)
                     /locus_tag="PKJ61_05420"
     CDS             complement(19863..20252)
                     /locus_tag="PKJ61_05420"
                     /inference="COORDINATES: protein motif:HMM:NF027567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOC13295.1"
                     /translation="MIVRPPAGSVCMLTTRAVDGRLRSRPVTVFHGADDAEVTIFTAA
                     SARKVTDISSDPAVTLAGPVADGWWSAEGSASIDQDATSELAAAGIPTAPYSCVLRVR
                     LQLVRQWTMHSSQPWDNTVEEWELTSR"
     gene            20693..21808
                     /gene="pdhA"
                     /locus_tag="PKJ61_05425"
     CDS             20693..21808
                     /gene="pdhA"
                     /locus_tag="PKJ61_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HOC13296.1"
                     /translation="MVGGDVPIQLLDPSGRRVEHPDFAFTGTDEEVAGYLRDMVLARR
                     IDTEATALQRKGELGLWPPMLGQEAAQVGSARATRAPDEIIPSYREHGVAWCVGIDPL
                     ELLGTFRGTAMVDWDSADKRFHLYSFVIGTQTLHAVGMAMAMQRDGLVGNQDPADDGA
                     AVVYFGDGATSQGDVNEAFIFAASYAAPVVFFCQNNQWAISEPIKLQSKIPLYRRASG
                     FGFPGVRVDGNDVLAVKAVTDWALDRARRGEGPTLIEAYTYRMGAHTTSDDPTKYRLA
                     AELDEWRAKDPLARVQAYLLASGAIDQAWLDALAAESDALAERLRAGCVSLPEPQLAE
                     MWGRVYADTPDLLHRQREEYLAYEESFSDGVGSGGVR"
     gene            21805..22785
                     /locus_tag="PKJ61_05430"
     CDS             21805..22785
                     /locus_tag="PKJ61_05430"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020577849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HOC13297.1"
                     /translation="MTNMTMAKALNAGLRRALGADPKVLLAGEDIGKLGGVFRVTEGL
                     QAEFGRDRVVDSPLAESGIVGTAVGMALRGYRPVVEIQFDGFVYPAFDQIVSQVSRLR
                     FRSHGRLSVPMVVRIPYGGGIGAIEHHSESPEAYFAHTPGLKVVTPSNPSDAYWMIQQ
                     AIAADDPVIFFEPKRRYHEKGEVDPDGESAPPSLYQARVVKPGADATLLSYGPMVRTC
                     VEAAVAAADEGRNLEVVDLRSLSPLDDATIFDSVRRTGRAIVVHEAVGTLGLGAELAA
                     RIQAECFYDLQAPVLRVTGYDLPYPPSRVEDGYLPDLDRVLDAVDRSLAY"
     gene            22796..24295
                     /locus_tag="PKJ61_05435"
     CDS             22796..24295
                     /locus_tag="PKJ61_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HOC13298.1"
                     /translation="MREFKLPDPGEGLLEAEIVSWKVAVGDTVAVNDILLEIETAKSL
                     VELPSPHAGVVSAILVAEGDTVEVGTPIIRIGDAGEAQESSQPTVIPASQPNVIPAQA
                     GISEATPTTETAAVTGNIQDELGEVPVAATDKPTEPPAQSGAMLVGYGVKPGAVARRP
                     RKGPGVVAVPETDQVQESYDTANPVSRRVDEVTPTSRVSAQPSGAHLPPPGRPPAATP
                     PDGPPRAKPPVRRIARDLGIDLNGIEGTGPGGTITAEDVVAAAGGPSADRRVPLRGVQ
                     RQMFRTMTESLKVPQATAMVEADVTGTMELLETLKSRREFTGLRVSPLLVFAKAACMA
                     IARNPSINSGYDEATDEIVLHGAVNLGIAAATPRGLVVPNIKGADRMNLLELAQALNQ
                     MVAVAKEGRVQPGDLASGTFTITNVGVFGVDGGTPILNEGEAAIMCLGTVRRKPWVVG
                     TGDEERIVPRSVCMVSLTFDHRLVDGEAASKFLADVATIMAEPGLSLLF"
     gene            24305..25192
                     /locus_tag="PKJ61_05440"
     CDS             24305..25192
                     /locus_tag="PKJ61_05440"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017935075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HOC13299.1"
                     /translation="MSTRSEVLVSISELPDAMRAGAQLLDVRWRLGEPHDAGYQRFLD
                     GHLPGARFLDLEAVLTGPLTDPLLGRHPLPDADQLAAGLGALGIEPELPVVVYDEPGS
                     FAAGRAWWVLRWAGLSVRVLDGGVKAWTAAGGELVSGAAPAVEPTQLRLTVGHLPTLT
                     ADEVAAFDGVLIDVRAPERFRGEVEPIDPKPGHIPGAINRPVGLLWDANGLLPSDDEL
                     RKLTHPEPVDGSEPTLRHPGGGRDLPTGNRIAVYCGSGVSAAQTMLALAALGIEAALY
                     PPSWSGWSADPERPIATGE"
     gene            25196..26314
                     /gene="alr"
                     /locus_tag="PKJ61_05445"
     CDS             25196..26314
                     /gene="alr"
                     /locus_tag="PKJ61_05445"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016454988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOC13300.1"
                     /translation="MLAPPTLATTRLDQIQANLSAIRGRVGDRQVLVAVKANAYGHGA
                     VAVSQMIEATGAADWLGVATVAEALELRASDIELPILKLSVARGPEVEAAVAAGVALC
                     VVDAASIVEAADAAVRQGVVAELHLKIDTGMRRIGAEPLDAVRLAEMVDATSGVRLGG
                     LFSHLPVSDVPSGVEFTRAQTALFAEVAARVETARGPVPLKHLANSGAVLGHPNTWLD
                     LVRPGIMAYGAYPDPAAERTVPLLPGLEWTSRVTFVKPIRAGETVSYGRTWTAERDTW
                     IATVPVGYGDGYSRLLSNRGRMLIGGRSYPIAGRVCMDQTMLDLGPETDVAVGDEVVL
                     IGRSGDEEITTAEIAELMGTIPYEVTCLITQRVARAYA"
     gene            complement(26529..27206)
                     /locus_tag="PKJ61_05450"
     CDS             complement(26529..27206)
                     /locus_tag="PKJ61_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020574984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HOC13301.1"
                     /translation="MTNEGIGKQVVARAPAVVGGMLRSIVEFAIDGNSSFPGAKATAA
                     KALQTKGEREAAIDSLVTQHIGLASAQGFLTSVGGLLTLPVGLPANIAGMGVLSVRMI
                     ASIAHLRGYDVDDRRVRAALTLAMLGDDEVRRMVADGRLPATPLAIATAPMFDPSLDQ
                     AINERVMGSLAGRLGGKHLAVVVVRRIPLVGGGVGAAVDGWLTFGLAAYAKREFVARH
                     QASIPRQ"
     gene            27407..28096
                     /gene="aat"
                     /locus_tag="PKJ61_05455"
     CDS             27407..28096
                     /gene="aat"
                     /locus_tag="PKJ61_05455"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002514166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HOC13302.1"
                     /translation="MARSGLFGPAREWPDQDLIAFSDEFDETLALEAYSCGVFPMPIG
                     QYSASEMGWWSPLRRGVMPLDALRVTRSLRKSAKRYTTTIDQAFDEVLARCADPKRPH
                     GWIDDAVRRVYTTLHEAGRVHSVEVWDSSGRLVGGLYGVCIGGLFAGESMFHDPEHGR
                     DASKVALLRLVEFLSDEHAEDRIIDVQWQTEHLASLGIIEIDRDEYLNLLDEALAVPE
                     PHWPAREERHA"
     gene            28089..28952
                     /locus_tag="PKJ61_05460"
     CDS             28089..28952
                     /locus_tag="PKJ61_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020574981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HOC13303.1"
                     /translation="MPELPEVEALRGFLERTCVGRTVARVELAAFSSLKTYTIPLSAL
                     AGLEVDAVERRGKFVCLSIGGLWLAFHLARAGWLTWSDAMPDAPARPGRGPLAARMIF
                     DDGSGFRLTEAGTQKRLALYLVNNLDEVPGIASLGPDPLAAEFTPEVLAGILATAGRA
                     QLKGVLRDQRIIAGIGNAYSDEILHAAKLSPFKPSNGLDEVELAALYAAIRDELSAAI
                     QRSAGLAAKDLKGDKKLNLRVHGRTGQPCGVCGTTIAEVSFADSSLQYCPGCQTGGKP
                     LADRRLSRLLK"
BASE COUNT         4607 a         9611 c         9894 g         4826 t
ORIGIN      
        1 agtcgaggcc aggttcagct cggacagctt gctgagcttc tccagcgggg cgatgttgcc
       61 cagcgggttg ccggacaggt tcaatgccgt ggccgcggtc agcaactcgg caccggtgag
      121 atcggtgatc ccgccagcgg cgcaatcggc gctggtaacg gtcccgagtt cggcctcggt
      181 gggcacgtcg gacgcgctga gcccgacggc ttcggccaga caggaccgca ggttcgcgtc
      241 cggtacgtcg gcggcaccgc tgaccggggc ggtcaccggc tgggtgatcg tgccggcgaa
      301 gaactcggtg ctgctttccg catccttggc ggagaacgtc caggtgtagc tgccctcagc
      361 cgggtagcgc accttctggt ccgtcaccgt gatgccgtcc ggtgcggtca gcgtcgcggc
      421 cgtcccggtg ttggtgcgcg gcttcggtgc ggaagtggtg aactgcgccg ggacggactt
      481 cgcggtgacc acctggccct gtgccttcaa ccccaggtaa cccagattgg tcaggccgct
      541 gagtgcggac aggtcgccga tctggttgta ttcgaggtcg acatcggtca gtttggtgag
      601 cccggccagc ggactgacat cgctgatccg attgctgaac gcgccgaaac cggtcagctc
      661 ggtgaggccc tgcaccgggc tcaggtcggt gatctggttg ttgctaacgt agagcttctt
      721 cagcgacttc agcccggtca ccggctccag tgaggtcagc gaagaattgt tcgccaccag
      781 ccgggtgagc ttggtcatgc cctgcacccc gtccagcgag gtgaggtccg cgccggtgtg
      841 gccgaacttc tgggtgacct cgaggttggt caggtttacc agcggggcga gcgcggagat
      901 cgaactgatc gggttgtagc tgagcagcag ctcgtccagc ccggtcagcg aagcgagcgc
      961 ggacggatca ctgatcgcgt tgcccggcag gtagagcttg gtcagcttgg tgaggccggc
     1021 cagcggcgcc acattgctga ccgcgttggt gctcagcacg agttcggtgg cgttgacgag
     1081 gtgctcggct ccggtcagct cgacgatgcc ctggtcgatg caggtgacgc tggtcagcgc
     1141 ctcaagctgg tccgcggtga tcgcagcgga cgtgtcctgc ttcagcaagc cggccaggca
     1201 cgccttgaag ctcgcgtccg gaacggcgac ctcgaccacc ggtggtgggg cgtccttcac
     1261 gacctgggtg atcgtgccgc cgaaggtgac gccctgcagg ctgtcggtga agctgaacgt
     1321 ccagttgtag ctaccggcgg acgcgtaggt cactttgctg ttcgaagtgg tgaccccagc
     1381 gggtggggtg gcgaccaggg cgctgccgtc cttcttggtg acggccggtg cagcggtctc
     1441 ctgggtggca gtcgcgtcgg ccgctgcgat cgtctgcgtg gtcacggcga gcttggtgta
     1501 gctcttggac ttgaccggcg acaggtcgga gatctggttg ctgctgagat cgacgttggc
     1561 caactgacta cccgccgtgg ccagcggggt gaggtcactg agctggttgt tggtggcggt
     1621 caggttcttc aagctggtgt tcccggcgag cggcgccaga ctgcggatcg cggtgtagtt
     1681 ggcggtgatc gcggtcagcg cggtcagctt gttcaccccg gccagtgtct gcagcttggc
     1741 cttggtggac gcgaggttct gctgcagctg gatggtgccc agcttcggca gcgtgccgag
     1801 cccggcgatc gaggtgatct cgttcagcga gaggttcgcg gtggtgacat tcaccagcgc
     1861 ggagatcggc gagacgtcgc tgatcgagtt gccgttcaga gtcaggccgg tgagcccggt
     1921 ggccaggttc agtccggtca ggtcctgcac gcccttgttg gcgcagctga gcgcaccggt
     1981 cagcgcggcg agctgggcca cggtgggctg aacggtcgtg tcggcgttgc cgatccgggt
     2041 gttgaggcag gcacgcaggg ctgcgtccgg gaccgtgtcc gcggtgtcgg tgactaccac
     2101 ggtcaccttg ccgttgaaat agaagtcgtc gggatcgcgg aacgtccact cgtagctgcc
     2161 gggaccggcg taggtgaccg tcgtgccact gaccgtagcg ccggcaggtg tggtgggcac
     2221 gaccaggctg ccggctgcgt tgacgatggt cggcacgatg gcggcgacac cggtggcggc
     2281 ggccgcggcc accgcctgct ggccgtagcg cgtcccggaa ccggacgagt aggcgggcag
     2341 ggtgccgagc acggaaaggt cgcgcagctt gttgccggcg agcgtgaccg cgctcagctt
     2401 gctcagtgcg ctcagcgggg cgatgttgct gagctggttg ttctccaggc cgagggagaa
     2461 gatcttggtt gaactcgcta gcccagcgat gttcgtgatc gagttgccac cgaggaagag
     2521 cttggtcagg ttggtcagcg ccgcggtgcc ggtgacgtca gtgatgccct tgccggtgca
     2581 ggacagttcg gtgatggtcg cgagctgttc ggcagacgga tcggcatccg cggccagccc
     2641 cagcttggtg ttgacgcagg cgcggtagtt cgcgtccggc atggtgtcgt tgaaggtagc
     2701 ggcggacgcg gtgactggcg gcaaggccag gaaggtgacg ctggcggcta ccgcgaccgc
     2761 gagccctctg agtcgtgaca tgtgaacctt tcaagtacgg ctcacgcacg gcatcagtga
     2821 cgcagtagca gtggttcagg cccgattccg cgcgggagcg tgttccgtta cgttgggcat
     2881 cggtccggct cctgcgtggc agttacggtt gcgggtcagc ccggggttcg cacccggttc
     2941 tccgtccaac gtgctgaggc tatcagtcaa tcggcagtcg ctgagttcga ctccggctcc
     3001 ggcgaaacct accgtcatcc cggcaagatc tactgtcatc ccggcgaagg ccgggatctc
     3061 tcggtctgat cggtggatcc gggctcagcc cgcgttcggc gggaggatca tcgtcgtcgg
     3121 gctgcggacg ccggccttcg ccagtgcgtt caggctgcgc tccagtagcg agcgctgcac
     3181 gccccagtgc tggttggtgg cggtcttcgc ggtcacgcgg atgctcatcg tcgtcgcagt
     3241 gacggcattg accccggcga cggcgggctt gtccagcagc acctcggcgt agtcggggtc
     3301 ttcggcgatc tcggcggcga cctcctccag gatgccgatc accttcgtag cgtcctcgtc
     3361 gtaggccacc ggcacgtcca cgatcgcggt cgaccagccc tggctctggt tgccgatccg
     3421 caagatttca ccattgcgga cgtaccacac ggtaccggtg ccgtcgcgca gccgggtggt
     3481 tcgcagcccg acctcctcga ccgtgccggt gacctcgccg gtgttgacca ggtcgccgac
     3541 gccgtactgg tcctccatga tcatgaagat gccggagaga aaatccttca ccaggctctg
     3601 cgcgccgaag gcgagcgcga cgccgccgac cccggcggac gtgagcagcg gccccatcgg
     3661 aatccccaga atattcagga tggtcaaggt cgcgatcgtc cagaccacca ctccgatcac
     3721 gctggcgatc acggagccga gggtcgccgt ccgctgttcg tagcgctcct ggttggccag
     3781 ggtgaccgtg ttcagcagct tctccgccac gctcaactcg cgctcgcggc gcttctttcc
     3841 cttggccagc gcgcgattcg tgatgccgcg cacgaggcgc accagcagga accgggcgac
     3901 gatcgcgacg acgatcacca cgccgatgga ggtcaggctg ttgaccggaa tccaggtggt
     3961 gaagtcgggc atgggcccat tcttgcccat cgttcgtgaa cgcggccgtc cacttggctc
     4021 ctgtcgggat ttcggagtcg gtcggtgctc cagggcccaa gtccgggtat tcggccaacg
     4081 gtggcttcga gatcgcgaca cggaatagtt gcgcgcgcag caaggttgag cagatagttg
     4141 aatcaacaac catttggagc aggaaaatgc agctgggtat cttcagtgtc ggtgatgtga
     4201 cgaccgatcc gacgacggga cggacgccga ccgagcatga gcggatcctg gctgcggtgg
     4261 cgattgcgaa gaaggcggag gagatcggcc tggatgtgtt cgccaccggt gagcatcaca
     4321 acccgccgtt ccatccgtcc tcgccgacga ccctgttggg gttcatcgcc gcgcaaactt
     4381 cgaagctgat cctgtcgacc gcgaccacgc tgatcaccac gaacgatccg gtgaagatcg
     4441 ccgaggacta ctcggtgttg cagcatctgg ctgagggccg ggtcgacctg atgatgggac
     4501 gcgggaacac cggcccggtc tatccctggt tcggccgcga catccgtgac ggcatcagct
     4561 tggccatcga gaactacgcg ctattgcgga aactgtggcg tgacgaagtg gtgaattggg
     4621 aaggtaagtt ccgcaccccg ctgcaggggt tcacgctggc gccgcagccc ttggacgggg
     4681 tgccgccgtt cgtatggcac ggctcgatcc gttccccgga gatcgccgag caggccgcct
     4741 actacggcga cgggttcttc cacaacaaca tcttctggaa cctgtcccac accaagcgga
     4801 tggtgaacct gtaccggcgc cgcttcgaac accacggcca cggccccgcc gataccgcga
     4861 tcgtcgggct cggtgggcag ttcttcatgc gccgcaactc ccaggacgcc atcaacgagt
     4921 tccgcccgta cttcgacgtc gctccggtgt acggctacgg gccgtccctg gaggagttca
     4981 tgaccgagac tccgctgatc gtcggctccc cgcagcaggt cctcgaacgc accctgtcgt
     5041 tcacggacgc ggtcggtcac taccagcggc agctgttcct gatcgatcac gccggcctgc
     5101 cgctgaagac cgtcctggag cagctcgacc tgctcggcga gatcctgccc gaactccgca
     5161 agggctacgc cgaaggccga ccagcgcaca tcccggacgc gcccacccac tcgtccctgg
     5221 ttgccgcaag ggacgcccgg agcttgggtg aggctgcaga cgcggtctcg accggcgcga
     5281 ccgcccgcgg gttcgtggac gaggtcaccg gccgctcgcc ctacgacact gcgaccacca
     5341 gccaaggagt agagcgatga ccagcatcgt cgtggtcagc gccggcaccg gcacgccgtc
     5401 ctcgagccgg ctgttgggtg agcggatcag tgacgccgta gtcgtcgcgc tcgcagaagc
     5461 caacacccag gccgaggtca ctcacattga actgcggacg atcgcggtgg agttggcgca
     5521 gcacctcacc acccgcgtcc ccggcgccaa cctgcggcgg gcgctcgctg cggtgcgggc
     5581 cgcggacggg ctggtcgtgg tttcgccggt gatgaacggc tcctactccg gcttgttcaa
     5641 gctgttcttc gacgcactcg aggaggacgc gctgcggggc catccggtgt tgctggcagc
     5701 gtccggcggc acggcgcggc actcactgat gatcgaccag gccatgctgc cgctgttctt
     5761 ctacctgaaa gcgctggtag caccgctctc ggtgttcgca gcgaccaagg actggggcac
     5821 ggccggtgga aagctcgaac gccggatcgc gtccgccgcc caggcgttcg cgacgctggt
     5881 gctgtcggcc gacccgatcg agaccacaga cgaattgacc gtcaccgatt tcagcgaact
     5941 gcttggcggc tgactacttc ttcacgccct tcagcgaacc cttcacgccg cccttgggtg
     6001 aaccagccga acacacgatt cggtgatcgg cgaccttctc ccagagctcg gcggacgcga
     6061 tgaagtaggt cacctcatac ttggactgtc cggcggcgac cccgacgaac ttctggaagc
     6121 cagccaggca cacggacgcg aacagcttct ccacctcggc gtcggtgggc gccttcttgc
     6181 ccttcacgtt cgaggcggcg aagacctcgt agtggtgctc gctcgcgcag tcgatcagct
     6241 ccagctctag ggccggcacg tcctggggcc cggtgcactg gcccttcttc gggaagacca
     6301 ggaagtcacc cttggctgag ccggtcaggc caccgtccgc ggaaccgacc aggcaggtga
     6361 tcactcggtt cgccggcgtg gcccaagcca cctcgtccgg gacgacatag gccgatgagt
     6421 agcgcgaata accgggctca gcgccgacgt agtcggcgaa gccgtccgag cacttcgtca
     6481 tcgcctgctc gccgagcgcc tcggcacccg ggtaggcgtc gccgctgatc gggacgcgcg
     6541 cctgcacctc gtagtagtgc ggctgcgcgc acggaatcgc ggtgaggctg gcgatcgatg
     6601 cgccgctcag gtccaccgga ccggtgcact ccccgacctg tggcacggac accgtcgccg
     6661 ccggcacctc cggggtgacc gattcggccg gcgtcgaagg cccggccgaa gacgccggcg
     6721 cactggttgg ggtggacggg ctcggcgccg tcgtggacgt gcatcccgcg agggcgagcc
     6781 cgagcgcgag caccaccgcg gcgcgcctca actgcatctg tcaccctcct gtcgactccg
     6841 cccatggtaa tgggccctgc actggtgggc ccggcctcta gtcttggcag cgatgcgagg
     6901 taacgacgtc tggtttccac ccggtgccac ccggcacgac tgggtgctcg acgccgcgct
     6961 cgccgctggc ctcggcctgt tgacgatccc gccgtacggc gtccgcgggt tcgccgaagc
     7021 gttcaccacc gggcagttcg ccagcgccgt actgatgatc ggtgcgctgg tgttccggcg
     7081 gcactcgcca ctgctcgcac tggcgggcgt gagtgtcgca gcgttgatgc aattggtgct
     7141 ctcatcaacc ccgatggcca ccctggtggc ggttccggtg gtcgcctact cggtcgcccg
     7201 ctggattccc ggacggcagg cgcgaatcgc gctcgccatc ggctcgatcg gcgccgtcct
     7261 cggcccggtg cgctggatcg tcgaagaccc ctactacctc agcctgcgcg acctgatctg
     7321 ggtggtgctg gcttggttcg tctgcatggg gctggtggtc accccgtacg cggtcggacg
     7381 ccgggtgctc gagtcgacgc agacccacca ccaccgcgtc ctggccgcgg aggagcgcta
     7441 ccggacgacg ctggcagagc gggaccagga agctcggctg gccgaatcgc gggcccggac
     7501 gcagatcgcc cgcgagctgc acgacatcgt ggcgcactcg ttgtcggtga tgatcgttca
     7561 ggccgaaggc ggacgggcga tggcgacgaa gaagccagag gctgccaccc aggccctgac
     7621 cacgatcgcc gagaccggac gcgaggcgct cagcgagatg cgccggatcc tcggagtgct
     7681 gcgctcggag ccggagatgc tgccgcaggc cgacttcgcg ccgtcgccgc ggctgaccga
     7741 cattcccgat ctggtcgcgc gaaccagcga ccgggtgcgg ctgaccgtgc agggtcagcc
     7801 gccgcgagtc tcgccggcat tggagctgac cgtgtaccgg gtggtccagg aagcgcttac
     7861 gaacttcctc aagcatgccg gtccgcaggc cactgccgcg gtgacgatca cctacggcat
     7921 gcgggagatc aacatcgacg tgatcgacaa cggtgtcgcg accccgtccg cggtgccgca
     7981 gaacccggga cacggcctgc gcgggatgag cgagcgggtc gcggcgatgg gcggacggct
     8041 gctgaccagg gcgcgtccga ccggcggctt tcaggtgacc gcggtgctgc cgctgccctt
     8101 atacaccaca gcgcaggagg atgaccaacg atgactatcc gagtgttcct ggccgacgac
     8161 caggagctgg tgcgcagcgg cttccgcatg ctgatcgagg ccgaggatga cctgcaggtg
     8221 gtcggtgagg ctccggacgg cgccggcaca gtggtggggg tgctgcggca tccgtgcgac
     8281 gtggtgctga tggatattcg catgccggtg atggacgggg tggaggcaac ccggcggatc
     8341 gtcgcttccg gtgtgccgac ccgggtactg gtgctgacca ctttcgacct ggacgagtac
     8401 gtattcagcg cgctgaaggc cggggcttcg gggttcatgc tgaaggacgc gcgtccgtcc
     8461 gacctgctga atgcgatccg gacggtcgcc gccggcgaag cggtgatggc gccgtcggcc
     8521 acccggcggc tgctcgacca tgtggtgccg aggctgccga acaaccccga aggccccaac
     8581 cctcgcctgg atgtgctcac cgaccgcgag cgcgaggtgc tggtcgagat cgccgctggt
     8641 gccaccaacg ccgagatcgc agccaagctc tacatggccg aaggcaccgt taagactcac
     8701 atcacgcggc tgctgtcgaa gcttcaatgc cgcgaccggg tgggtttggt cctgttcgcc
     8761 tacgagaccg gtctggtgcg ccactagccc aaatcggtcc cgccgagctt tgcgcgggac
     8821 gagccccacc gttggtggag aggccggttt cacgtcatcc cggcgtaggc cgggatctct
     8881 caccctggcg ggccaaccgg gacagtccct ttcgccgccg ccgtctcaga gcagcttcag
     8941 tagcgcgtcg ggattgtggc ggtaggtgtc caggctctgc cggccggcgc tgaggtcttc
     9001 cagggtctcg gtgaggacgc ggttcaccgg ggtcggcaca ccgagttcat ccccgtggcg
     9061 caccaccgct ccgttcagga actgcacctc ggtctggccg cgtccgccgt gcaggtcgat
     9121 gtggaaggac ggcatcttgt cgccgcgtcc gccgccaaga gccctggtca gcgcaggttg
     9181 ggcgatgaat cgcggcagcc gctgggcggc caaggcgagc gcacgcaccg gcgtccccgg
     9241 tagatccacc ggacggtagc cgagcgcggc catcacggcc aggcactcgc gcagcaccgc
     9301 gacctcgatc gcgtaggtgc ggcggtcggc gaacagtgcc ccgaccggct cgtccagaat
     9361 cgcggacgtc gcgttgccgg tgaggttggt gagcagcttc gaccacttca tcggtccggg
     9421 ctgggggtag ccgacaccgt tcaggtcggc atcgttgagt gcgctgacca gtgcctcgcc
     9481 gagcgggtgg tccagggcga tgccgatgcc gcgacgcttc tcctcaatca cctgacctat
     9541 gcccggcttg ctgaccgcgg tggtcacggt gccggtgatc acccggtcgg cgccgagcgc
     9601 ggcgaccagt ttcggctcgt tgtccacgcc gttctgcagc gagaggacgg gcggcaactc
     9661 ggcgccggtc gcctgcaggc cggtgagcgc tgattcggtg tcgaacgact tcaacgcgaa
     9721 gaccatcgcg tcgtaaggtc ctgcggccag ggccgcgtcc gccgaatcga agatggcgaa
     9781 atcgcggacg gaggtagtcc ggttgtcccg ggtcagccga agaccctgct cggcgatgat
     9841 ccgggcggct tcgggttgct cgctgaaggt gacgtggtgc ccggcggcag ccagcgaacc
     9901 gccgacgtag gtgccgatcg caccagcccc aaggaacagc aggcgcatgt cactcctctc
     9961 gattggcccg catcagtcta ttcacggctg ttgctgactc tgatcggcgg ttcagcccgt
    10021 ccagtcctgc gccagcaggc ccatgacgtg gcaatccacc cactcgtcgt cgaaccgcag
    10081 cgcctgccgc ttggtgcctt cgagctggaa cccgaccttc tcgtacacgt ggcgggcgcg
    10141 cgggttgaag gcgtatacct cgagttccac ccggttcagt ccggacgcga acgcgtgccc
    10201 gacgctcagc cgcaccgctt cggtgcccaa tccgcggtcc cgggcaccgg tgatccagat
    10261 ccggaagccg caactgaggt tgccctcgtc aagatcgttc agcaccgact cgccgacgat
    10321 cttgccggtc gagttctcga tgaccaccca gacgatgcga tcgggcgcgg tcgcccagcg
    10381 gccgtagatc tcttccaggt cggcgaccgt ccactgctct tcgtcggtcg cggtgctgga
    10441 gtgcaccgat ccggtgagtc ggctgacctc ggcgtccgcc atggcctggt gcaggatggc
    10501 tgcgtcggcc gctgcggctg gccgcagggt gaccagttcc ccggcgaggg tgggcttgac
    10561 ggtgaagtca ggcaccctgc tcctggtcga gccggtcaag gatccacgag cggacgagtg
    10621 tggacgcggg cagatgccgt gcgtcagcga tcttctggat ggcgttgctc tcggacggac
    10681 tgagccgcac cgagtagacg agggtgcggc gctgattgcg ggcggctgtg ttggaggtca
    10741 tgtagtacaa ggtaatactc caccagtcaa ttgaacagat cagtctgggt tggtcgttgt
    10801 cgccagtcgg caaccagggt gcgcagccgg acggcatcct gatccccgat caactggcca
    10861 gcgatggcgg cggcttcgcg cacccgccag agctcatccg ccgcccgggc ggtgagcagt
    10921 tcgaccaacg aaagatcgtc cggtgcttcg aggatcagcc ggccgacagc agccactccg
    10981 cagcacgcga ggtactcatc cgacttgctc gcgaagcgta gagccacttc gcgaggcagt
    11041 acgtcggccg cggctgccag cagggtcagg ttcgagcgcg gaccgggcag gccactgcgc
    11101 cgccatgcta gtgagaccgc cgccccgggt gggaagcggc ccctcgtgac gcgcttggct
    11161 tcgatcctca gccgagcgct cctcagcgac cgtttccggg acgcactcgg gcgggatgac
    11221 cgcggcacgg ctcagtgcac tgcgtccgcg tcctcggcgt actcgggatc ggtgttcacc
    11281 gcagcgcggc gcagcgtccg caacgacgcg agcgcgatcg cggctgcgac gagggcgaag
    11341 gccgcgaccg acaccaggct ggcgtggtag cccgaagagt cgatcagctg tccagcgaca
    11401 ctcgaaccaa tcgaggcgcc gatcccgata ccagtgccca tccacgagag accctcggtg
    11461 aggcgctcac gcggcaccag ccgtccgatc aacgtgttgc cgttgatcag cgtgggcgcg
    11521 acggtcactc cgacgaggaa cccgaccgcc gaaagcagca ggatcgagtt cgccagcagc
    11581 aggcccagcg tgctcgcaaa cagggccagc acgcccacca cgaaccgacc cggcagcggc
    11641 gaggaccagg tgcgcgagcc gtagtagaat ccggccagcc cggacgcgag cgagaacacc
    11701 gccagcacca ccccggatgc cgcccggacg tcccaggagg tggctgccgc gaccacgctg
    11761 acatcgatgg cgccgaagac gcagccgatc agcaggttca ccgcgaccac tgctccgacg
    11821 cccggcgcca tcaggatgag ccgtcgccac aacggggttt gctgctgcga ggccggtcgc
    11881 ggcatgaccg gcggctcggt ttcgcgctgc gagtagaaca gcttcgcccc caccagagac
    11941 aagatgatcg gcgcaaccag accggccgcc ggatgcacgc cgaccgagag ggccgtagcc
    12001 gccaccggac cgatcacgaa ggtcagttcg tccagcgtcg attcaagcga gtaggcggtg
    12061 tgcaattgcc cggggtcgct ggtggcgtgg ttccagcggg cgcggacgag cgcgccgggc
    12121 gcgccaccgg tggcgccgct gatgatcacc ggcgggaaca gcgtccatgc cggcaactgc
    12181 agcacggcga acacgacgac gaccatgagc atggtcgccg aaaccaccac cgcggggtac
    12241 atcacccggc gctggccgta acggtcgaca aggttcgaga gcaccgcggt gccgattgcc
    12301 caagccacgc cgttcgctgc cgcgagggca ccggccaggc cgtagctgcc gtagagcgcg
    12361 gagaccatca ggacgatgcc gaggcccatc atcgccccgc ccgatcgggc cagaacgccg
    12421 gccgcgctga acttcaatgc ccccgggatc gcgaggattt cccggtaaga acgcatccaa
    12481 cgagtgtgtc acattgaacg cgctctcatc gaatcgctaa cgcttgtgga atcgtttcgg
    12541 acgcgggcct tcggcgcccc cgttgaaccc ggatgtggga cggcgcgaac gcccgctagg
    12601 ctctgccgac gtggctcggg gacgcaggct ggtggcggcg gtagcggtcg tgctgctgag
    12661 ttgggcctcg ccggggctgg ccgaagtggc ggacgcgcgt ccgctcggcc tgatcagcga
    12721 gatatccccg ccggccgaga ccccgtctcc ggaaccgccg cccgctccgt ccccggagcc
    12781 gacgccggaa cctacgccga gtccgacccc ggagccgacg cccgaaccca cgcccactcc
    12841 cgaaccgacc ccaaccctga cgcccactcc gaccccggaa ccggactgcc gcggagcctt
    12901 ggacgatcgg aagtcagcga aggccaagcg cgccccgttc gtggtgtgcg acaccgtggt
    12961 gatctcgaag aagcatcggg tcacgtccgc ctacaagccg agcctcagcc gggttcaggt
    13021 gcctacctac gggctcagtg gggtctacct ccaagcaaaa gccggcaccc aactgaaggc
    13081 gatgttcgcc gcagcgaaga aggccggcta cacgctggtc gtccgctcgg cttatcgctc
    13141 ctacgccacg cagaagcgca tctaccgccc cggcgcgaag ctgaccgctc ccgccggtgc
    13201 cagcgagcac cagtccggac tggccgtcga cctcgcctgg aagcgcggac ggatcgtccg
    13261 gggctacacc ttcggtagct cgaaagctgg agcctgggtg cgcgcgcatg cggctgacta
    13321 cgggttcatc ctgcgctacc ccaaaggcaa gcagaagatc accggcatcc cgtacgagcc
    13381 gtggcacttc cgctacgtcg gcgagtcggt cgccaacggc gtccgacgca cgtccacgaa
    13441 gacgctggag cagtacctgc gaatcgactg aagccgactc agcgcacgcc ggctccgtac
    13501 agcacggcgg cgtaagcggc ctggccgagg aggttcggcg gcccggccgc agccggacgc
    13561 agcccgctga tcacccaggg caggccggta tcgcacaggc cgtggccgtt gaacgccctg
    13621 gcgacgtcga cgtacaccac gcgcccagcg atcgagtctt catcgggtcc cccgatcccg
    13681 aagaagttgg caccaaaatt acgcccgcgg tacctcttgc ggaatcgggc catgcccatc
    13741 gaaatgaggg ttctgcgggc tctaagcggg ctgagaacac ccatttcgac ggccgggccg
    13801 ccaacgaagg ctggtcggcc atgaccacca ggaccgtgct cgactgcgac tcacccgttc
    13861 ggacttcccg gttccgcggg aggttctcaa cgccgagcat gccgagttcc agctgcggcg
    13921 attacccttg ccaccggcgc tttcgcgggc tcaggacggc catgcggtcc ggatgctgat
    13981 cgataccttc atggatccgg cgaatgcgga cgcggtgctg gtcgccaccg gaccgctcac
    14041 caatgtcgaa accctgggcc gacggtaggt ccaagtcgca cctccgccga cgtccgtcgt
    14101 gaactcggtg acctgctcga acggctgacc gcccactacg gccgcgcgcc gatcctctac
    14161 gccaccccgg aggcatacga gcgctatctc gcggcgaact tcgtggtcaa cgacatctgg
    14221 atccgggaca tctggcgcga gcctgccctg tccgacggcc cgcgactgga ccttctggca
    14281 atacagcgac cggaatcgac tggccggcta ctccggcgag gaagccttca tcgaccagaa
    14341 cgtcttcgct ggagatcgcg acgcctgggc cgcctactgc cgctgatccg ctcggtgccc
    14401 aagccgccct gaacgcatcc gggtttcgac cgattgggaa gctccatcta cccttggagt
    14461 catgtggtcc aagcgtgaag tggtcgatta cgcgctgcag cgacgcaatt cgctgcaggc
    14521 gttgcggcgt ccacaacgct cgctggaaag cctctcgatc tgcgacgccg acccgctgct
    14581 gattcgggcg gccacacatc atggtgagga atcgagagtg ccctgcccgg tgtgcgaagc
    14641 cgagcggacc actaccttga actacgtgtt cggcgatcaa ttgggccagt actcggggcg
    14701 catcaaatcc accgtcgaac tcgacgaaat gcaatccgac tacggcgaat tcaaggtccg
    14761 cgtggtcgag gtgtgtgtgc ggtgcggctg gaacttcctg atcaccagct acctgctcgg
    14821 cgacggggtc acccgccgtc cgcctcggcg acaaccgacc gttgaggaca tttatggctg
    14881 atcccaagcg cccggcggcc gcgccgcact cacagggaaa gaacaagaag aagcgaatct
    14941 ggctgcgggt cctggcctgg accatgtcga ttctgctggt gctcggtctg gccggggtgg
    15001 gggtgatcgc ctacgcctac agcaccatcg aagtgcccga tccgaattcg gatttccaga
    15061 ccaatacgag tttcgtgtac tacgcggacg gaaagtcgaa gatcggctcg ttccaggtgc
    15121 agaatcggca gagcatcacc tatgaggaga tgccgcagta tgtgaaggat gccgtggtcg
    15181 cggccgagaa ccgcactttc tgggaagacc cgggaatctc cgtgaccggc ttattccggg
    15241 ccgcattggg attggtcggc atcagcacgg gtggtaccga atccggcggc ggctcgacga
    15301 tcacccagca gtacatcaag atcatgtacc tcacccagga gaagagcttc acccgcaagg
    15361 ccaaggagat cctgctggcc gcgaagatag gccaggagct gaccaaggaa gagatcctcg
    15421 agcgctacct gaacacggtc tacttcggcc gcggcgcgta cggcatccag gccgccgcgc
    15481 aggcctactt caacaagtcg gccgccaagc tcaccctcgc tgagtcggtg gcgctggccg
    15541 cgatcctgaa ctcgccgggc aatctcgacc cggccaacgg ggacgccgag gctgccgacc
    15601 tgctcgagcg ctaccagtac acgctcaacg gcatggtcga gctcggcatg atcaccgaag
    15661 cccagaaggc tgagatctat cgcaccctgc cggagttccc cgagcccaag accaactcgc
    15721 agatgggcgg gacgaagggt ttcctgctga gcctggtgca gaaggagctg ctccgggccg
    15781 ggttctccga ggaggagatc tccggccggg gcctgaagat cgtcaccacg tttgacaaag
    15841 ccgcccaggc cgcggcggtg gacgcagcca aggagaccgc gttgcaggcc gcgggcggcg
    15901 acaaaaaggc ggcggccaaa ctgcacccgg cgctcgcgtc catcgacaat gccaccggtg
    15961 gcgtggtggc cctctacggg ggcccggact acgtgaagag ccaatggaac tgggccacga
    16021 acccgcgtcc gactggttcg acgttcaagc cctacgcgct cgccgcggcg ctgcgcaatg
    16081 aatggacgct gaacgacaag ctgaacggca acaccttcac ccccgacggc gattcacgcc
    16141 cggtgcgcaa cgccggcggc gcgaactacg gccggatcac actgcagcag gccaccacca
    16201 agtcggtcaa caccgcctac accgacctgg tcagccagat cgagaacggg ccgcaggagg
    16261 tggtcacggc agccacggac gcgggcctgc ccgaatcgac cgactgggat ccgtccaacc
    16321 gcattccgct tgggcgtacc gaggtgagtc cgctcaacca ggcgtccgcc tattcgacgt
    16381 tcgccaacca gggcgttcac cgcacgccgc acgtggtggc cgaggtgttc gacgtccagg
    16441 gccgctcgat ctacgcggcg gacaccaatg gcagccagac catcgaaacc gatgtggcga
    16501 tcgatgtgac ctacgccctg accaaggtga cgcaggacgg caccggctac cgcgcgtccc
    16561 agctcggcta cgaggtggcc ggcaagaccg gcacctacta cgtggcggat gggggccgct
    16621 ccgagacccg ggcggtctgg ttcgtcggct tcaccaggca gctcaccaca gcggtgatgt
    16681 acgtgaaggg cgatcagggc accaccgacc tcggcagcgg ctggttcggt tcgggctggc
    16741 cgctgaccac gtggctggcg tacatgcgca cggcgatgga cggtaaggag cacgagggct
    16801 tcgatccgcc gaccgtccgg gtgtcgacgc agacaccgac cgcgaagccg tccaagaccg
    16861 ctgagccggc gaccgagaac ccgctgccga ccgagaccgt gcccaccgaa ccggtgccga
    16921 ccgtgaccgt cccgggcgag ccgactgtgc cccaaccgac gcagccgaca cagccgaccg
    16981 agacagcgtc ggtgccgagc gggcagccca cggtcaccga aggggcgcaa gccgccgtcg
    17041 accccggcgc ccagactgtc gccgcgggcc cgtaggatca cggggtgacc cagactccgg
    17101 cagcgctgtc gaacgtgctc ggcggcgcac tgggtaagca cgtgcgtccg ggtggggtgt
    17161 ggttcaaccc cgctccgtgg acatatctgg cactgacgct gtcctggttg gtgctcgcgc
    17221 tacggcagtt gccgtgccgc aacagctcgg acatgttccc gaacatgtgc tacaccgaca
    17281 tcacggtgct gtactactgg cgcgggcttc aggacggcaa gatcccgtac ctcgacagcg
    17341 atgtcgaata cccggtgctc actggcgcga tcatggaact cgggcggcgc ctggtcgtgc
    17401 tcttcggcgg acgcagtgag ccgggtctga gcagcaccga gatcagtcac gcggctgggc
    17461 tgttcttcgg catcaacatc gtgctgctgt tcggactgtt cgtggtgctg gtctgggcgc
    17521 acctgcagtt ccaccggccg tgggacgccc tgatgatcgc gatcgccccg gcgatctgga
    17581 cttccgcgct gatcaactgg gacggactgg tgctcgccct gaccgcgctg gcgatgcttg
    17641 cttggaaacg gaagtcgccg gtgtggacgg gagtgtggct ggggctgggc atcgcggcga
    17701 agctgtatcc ggtgttgctg ctggttcccc tggctgtgct gtgcctgcgc acgagccggt
    17761 ggcggccgtt cttcatcacc ctgggggcga ccgtagcgtc ctggctggcg gtgaacctgc
    17821 cggtatatct ggccacgcct tcgggttggt tgaatttctg gaccttcaac gttgaccgcg
    17881 gcggcgacct cggctcgatc tggtacgtgc tcggcctggc cggctaccag gtggattcgg
    17941 tgtcgaagct ggtggccgcg atcatggtgg ccggcacggt ggtgatctgc ggcttgctgc
    18001 tgctggcgcc acgccggcca cggttcgctc aaggggtctt tctgatcgtc gccctgttcc
    18061 tgatggtgaa caaggtctac tcgccgcagt acgtgctctg gctgctgccg ctgctggtgc
    18121 tcgcccgtcc acgctggctg gactgggcgg tgttctcgat cgcggagggc ttctacttcg
    18181 cggccatctg ggcgcacctg gacggtcgca gtgcgtccgg cgacggccac gatcggatgt
    18241 actggctctc ggtgctgctt cggatcgcgg tgcagttctg gctgtgcgtt cgcgtcgcgc
    18301 tcgacatggt gcgcccggag cgggacgtgg tgcgcgccgg cagcctcgac gacccggacg
    18361 gcggcatctt cgaccacgca tccgacgccc cctggctggt gcgcctgaac cagcgcttgg
    18421 ccgcccggtg aacggtgtcc ccgatcggcg cattttcatc ccggcgaacg gtgcccccaa
    18481 ccggcgcatt gtcatcccgg cgcaggccgg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18601 nnnnnnnnnn tcatcccggc gaacggtgcc cccaaccggc gcattgtcat cccggcgcag
    18661 gccgggatct ctaggtgcac gcgcggctgc cagccccacg acaagacgcc atgacaacta
    18721 gcgtccaccg ctcagccgac gcgcgcctgg tcgtgcagac ctggttcgcc acccggctgc
    18781 tgatggccct ggtcggcgtc tggctgatgc tgaccgaggg ccggaagttc tccgacctgg
    18841 tgggcaactg ggacgtccag cacttcctcg ccatcgccgc ggacggctac gtagcccgcc
    18901 acaacatcgc cttcttcccc ggttggccgc tgctgctgcg gctgacctcg acgaccgggc
    18961 tgccgataat gatcgccggc actctgctgg cgctgctcgc gtccggtttc gctgcggccg
    19021 cgctgtaccg catggccgga gccccggcgg cgatcgcctg gttgctggcc ccgaccgcgg
    19081 tgttcacgat ggtgccgtac accgagtcgg tgttctgcgc ggcggcgttt tgggcttggg
    19141 agcgcgccac cgccaagcag tggggtgcgg cagcggtgct cgctgcggtg gcggcggccg
    19201 tccgcgtctc tggggtgttt ctgatcgtgg cgctggcggt gctggcgatc acgtccgccg
    19261 gatcgtcacg ggacaagggc aaacggttgc tgtggctgct gttcccgctc tgcgtggtgg
    19321 tggcgtacct cgcctacctc tattcgatga ccggcaactg ggcagcctgg tacgaagccc
    19381 aggcggcagg ctggtcgcgc ggcttcacct ggccgtggga ctcactgcgg cacaccttcg
    19441 aggccttcgc gccgggtgct tacgccgacc atccggaatg ggcctgggtg ttccgcgccg
    19501 aggtggtgag catggcggtc ggggttgtgg tgaccctggg cagcctggtg cgccgacgct
    19561 ggggcgaggc gacctgggtg ggcctgcagg tggccgcgtt ctcggtttcg tactggttca
    19621 tgagcgtgaa ccgcgctgtg ctgctgtggt tcccgctgtg gacgtggctc ggcgaactcg
    19681 gcggcagccg acgcaagcta cccacctggc gcgcgattct gatcggcctg ctggtggccg
    19741 tcgccctggt gacccaggcc atctgggcct ggctgttctt caccggccgc tgggctgcct
    19801 agttcggatg gccgttccgg ccgcgtccgc tcacggtttc ggtagcaaac gtgaagcagc
    19861 ggtcagcggc tggtgagctc ccactcctcg accgtgttgt cccagggctg ggacgaatgc
    19921 atcgtccact gacgcaccag ttgcaggcgg acgcggagca cgcaggaata gggcgcggtc
    19981 gggattccgg cggcggcgag ctcactggtc gcgtcctggt cgatgctcgc cgaaccctcg
    20041 gccgaccacc agccgtccgc caccggacca gccagagtga ctgcaggatc ggacgagatg
    20101 tcggtcactt tgcgggcgga ggctgcggtg aagatggtca cctcagcatc gtcggcgccg
    20161 tggaaaacgg tgaccggacg ggaccgcagc cggccgtcga cggcacgcgt ggtgagcatg
    20221 cagaccgaac cagctggtgg ccgaacgatc atccacgtct ccttgtcaat cgcttgctgg
    20281 gatgcagggg actcgcgtcc gctgctcacg aggctaatct cccaccattt cggcgacagc
    20341 ggtcccacta cgtgaaacag ggtgtcgtag tggacggcat taccttagcc acgcgcctgg
    20401 cgagctgatc tgcgctctgg atctgtgaca ttgggcgaag ccagtcaccg gacgcgattc
    20461 tggcgtgcgg gcctgtgtgt gggtgtacgc gtaccggctg gcaggctggc tcaagcattg
    20521 acacctatgg ttacgtaacc gtaagttaga tgaaccaagg agcgtcagcc accaggagag
    20581 cccgttgacc gagcccgcga gcaacaccca ttccgcgagc ccaaaccgtc gggccgaggc
    20641 gagcagcgga gatcccgcca ccagcctcgg cccggacggg ctcaaccccg caatggttgg
    20701 cggcgacgtg ccgatccagc tgctcgatcc gtccggacgc cgcgtcgagc accccgactt
    20761 cgcattcacc ggcaccgatg aagaggtcgc tggctatctg cgcgacatgg tgctggccag
    20821 gcggatcgac accgaggcca ccgcgctgca gcgcaagggc gagctcgggc tctggccgcc
    20881 gatgcttggt caggaggcgg cccaggtcgg ctcggcccgc gcgaccaggg cacccgacga
    20941 gatcattcct tcctaccgcg agcatggcgt ggcctggtgc gtcggcatcg atccgctcga
    21001 actgctcggc accttccgag gcacggcgat ggtcgattgg gattcggctg acaagcgctt
    21061 tcacctctac tcgttcgtga tcggcaccca gaccctgcac gccgtcggca tggccatggc
    21121 catgcagcgg gacgggctcg tgggcaacca agacccggcc gacgacggcg ctgcagtggt
    21181 ctacttcggc gacggtgcca ccagccaggg cgacgtgaac gaggccttca tcttcgccgc
    21241 ctcgtatgcc gcgccggtgg tcttcttctg ccagaacaac cagtgggcga tctcggagcc
    21301 gatcaagctg cagtcgaaga tcccgctgta ccggcgagct tccggcttcg ggttccccgg
    21361 cgtccgggtg gacggcaacg atgtcctcgc agtcaaggcg gtcaccgact gggccttgga
    21421 ccgcgcccgt cgaggcgagg gcccgacgct gatcgaggcc tacacgtatc gcatgggcgc
    21481 gcacaccacg tccgacgacc ccaccaagta ccggctcgcc gccgagctcg acgagtggcg
    21541 cgccaaggac ccgctcgcgc gcgtgcaggc ctacctgctc gcgtccgggg cgatcgacca
    21601 ggcttggctg gacgcgctcg ccgcggaatc ggacgccctc gccgagcggc tgcgcgccgg
    21661 ctgcgtgtcg ttgcccgaac cccagctagc cgagatgtgg ggacgcgtct acgccgacac
    21721 ccccgacctg ctgcaccgac agcgcgagga gtacctcgcg tacgaggagt cgttctccga
    21781 tggcgtgggt tcagggggcg tgcgatgacc aacatgacca tggccaaggc gctgaacgcg
    21841 ggcctgcgcc gcgcgctggg cgccgatccg aaggtgcttc tggccggtga ggacatcggc
    21901 aagctcggcg gcgtcttccg ggtgaccgag ggcctccagg ccgagttcgg acgcgaccgc
    21961 gtggtcgact ccccgctggc cgagtcgggc atcgtcggca ctgcggtcgg gatggcgctg
    22021 cgcggctacc ggccggtggt ggagatccag ttcgacggct tcgtctatcc ggcgttcgac
    22081 cagattgtct cgcaggtgtc gcggctgcgc ttccgctcgc acggacgcct gagcgtcccg
    22141 atggtcgtcc ggattccgta tggcggtggc atcggcgcga tcgagcacca ctccgaatcg
    22201 ccggaggcct acttcgcgca cacacccggg cttaaggtcg tgacgccgtc caacccgtcc
    22261 gacgcctact ggatgatcca gcaggcgatc gcagccgatg atccggtgat cttcttcgag
    22321 cccaagcgcc gctaccacga gaagggcgag gtcgacccgg acggcgaatc agccccgccg
    22381 tcgctctacc aggcgcgagt ggtcaagccg ggcgcggacg cgaccctgct cagctacggc
    22441 ccgatggtgc gcacctgtgt ggaggccgcc gtcgccgctg ccgatgaggg ccgcaacctt
    22501 gaagtggtgg acctgcgctc gctctcgccg ctggacgatg cgacgatctt cgactcggta
    22561 aggcggacgg gacgcgcgat cgtcgtccac gaagccgtcg gcacgttggg gctcggcgcc
    22621 gaactggccg cccggattca ggccgaatgc ttctacgatc tgcaggctcc cgtgctacgt
    22681 gtgaccggct atgaccttcc ctatccgccc agccgggtcg aagatggcta cctgcccgat
    22741 ctcgatcggg tgttggacgc ggtcgaccgt tcgctggcct actaaggaag ctgtgatgcg
    22801 cgagttcaaa ctgcccgacc ccggtgaggg cctgctcgag gctgagatcg tgtcctggaa
    22861 ggtcgcggtc ggcgacacgg tggccgtgaa cgacatcctg ctggagatcg agaccgccaa
    22921 atcgctggtc gaactgcctt cgccgcacgc gggcgtggtg tcggcgatcc tggtcgccga
    22981 gggcgacacc gtcgaggtgg ggacgccgat catccgcatt ggcgatgccg gcgaggctca
    23041 ggaatcgtcc cagcccaccg tcatcccggc gtcccagccc aacgtcatcc cggcgcaggc
    23101 cgggatctcc gaagccacgc caaccaccga aaccgccgcg gtcaccggca acatccagga
    23161 cgaactcggc gaagtgccgg ttgctgccac cgataagccc accgaaccac ccgcccagtc
    23221 cggcgccatg ctggtcggct acggggtcaa gccgggagcg gtcgcccgcc gtccgcgcaa
    23281 ggggccgggc gtggtcgccg tgccggagac cgaccaggtg caagagtcgt acgacactgc
    23341 gaacccggtt tcgcgccgcg tggacgaagt cacgccgacc agccgggtga gcgcgcagcc
    23401 gtccggggcg cacctgccgc cgccaggacg tccgccggcc gcgaccccac cggacggccc
    23461 gccgcgcgcc aagccgccgg tgcgccggat cgcgcgcgac ctgggtatcg acctgaacgg
    23521 cattgagggc accggtccgg gtggcacgat cacggctgag gacgtggtgg ctgcggccgg
    23581 cggaccgtcc gccgatcgtc gggtgccgct gcgcggcgtc cagcgccaga tgttccgcac
    23641 gatgaccgag tcgctcaagg tgccgcaagc gaccgcgatg gtcgaggccg acgtgaccgg
    23701 cacgatggaa ctgctggaga ccctgaagtc acgccgcgag ttcaccggac tacgggtttc
    23761 accgctcctg gtgttcgcga aggcggcctg catggcgatc gcgcgcaacc cgtccatcaa
    23821 ctccggctac gacgaggcga ccgatgagat cgtgctgcac ggcgcggtca acctcggcat
    23881 cgctgccgcg actccgcgcg gtctggtggt gccgaacatc aagggcgctg accggatgaa
    23941 cctgctcgag cttgcgcagg cgttgaatca gatggtcgcg gtggccaagg agggacgcgt
    24001 ccagcccggc gacctcgcgt ccggcacgtt cacgatcacg aacgtcggtg tcttcggggt
    24061 ggacggcggt acgccgatcc tcaacgaggg cgaggccgcg atcatgtgcc tgggcacagt
    24121 gcggcgaaag ccgtgggtgg tcggcacggg cgacgaggag cggatcgtgc cgcgctcggt
    24181 ctgcatggtt tcgctcactt tcgaccatcg actggtggac ggcgaggccg cctcgaagtt
    24241 cctggccgac gtcgccacga tcatggccga gcccgggctg tcgctgttgt tctagagttc
    24301 aggcgtgtcc actcgtagcg aggttctggt cagcatcagc gaactcccgg acgcgatgcg
    24361 tgccggcgct cagttgctgg acgtgcggtg gcgtttgggc gagccccacg acgcgggcta
    24421 ccagcgcttc ctggacggac acctgcccgg tgcccggttc ctcgacctgg aggctgtgct
    24481 gaccggaccg ctcaccgacc cgctgctcgg ccgacatccc ttgccggacg cggatcagct
    24541 ggcggccggg ttgggggcac tcgggatcga gccggagctg ccggtcgtgg tctacgacga
    24601 gcccggatcg tttgccgccg gtcgcgcgtg gtgggtgctg cgctgggctg gtttgtcggt
    24661 gcgggtgctc gacggcggcg tgaaggcctg gacggccgcc gggggcgagt tggtcagcgg
    24721 cgcggcgcct gcggtggaac ccacccaact gcggctgacc gttgggcacc tgcccacgct
    24781 gaccgcggat gaggtggccg ctttcgacgg cgtgctgatc gacgtccgcg ctcccgagcg
    24841 gttccgcggc gaggtggagc cgatcgaccc gaaacccggt cacatccctg gtgcgatcaa
    24901 tcgtcccgtg ggcctgctct gggacgccaa tggactgttg ccgtccgacg atgaactacg
    24961 aaagctaacc caccctgagc ccgtcgacgg gtctgaaccc accctccgtc atcccggcgg
    25021 aggccgggat ctaccaactg gcaaccgaat cgccgtctat tgcggttccg gagtctccgc
    25081 agcccagacg atgctggcgt tggccgctct tggcatcgag gcggcgctat acccgccgtc
    25141 ctggtcggga tggtcggccg acccggagcg tccgatcgcg accggggagt aaccgatgct
    25201 cgcgccgccc acgctcgcca ccacccggct cgaccaaatc caggcgaacc tgtccgcgat
    25261 tcgcggacgg gtcggcgatc gccaggtgct ggtggcggtc aaggcgaacg cctatgggca
    25321 cggcgcggtg gccgtcagcc agatgatcga agccactggc gcggccgact ggctgggagt
    25381 ggcgaccgtc gctgaagcgc tcgagctgcg cgcgtccgac attgaactac cgattctcaa
    25441 gctgtcggtg gcgcgcggtc ccgaagtcga ggctgcggtg gcggccggtg tggcgctctg
    25501 cgtggtggac gcggcatcca tcgtcgaggc tgcggacgcc gccgtccgcc aaggcgtggt
    25561 ggccgagttg cacctcaaga tcgacacggg catgcgccga atcggcgccg aaccattgga
    25621 cgccgtccgc ttggccgaga tggtggacgc gacctccggc gtccgcttgg gtgggctgtt
    25681 ctcccatctc ccggtctcgg acgtgccgtc cggcgtggag ttcaccaggg cacagaccgc
    25741 gctgttcgcc gaggtggctg cgcgggtgga gacggcgcgc ggccccgtcc cgctgaagca
    25801 cctggccaac tcgggggcgg tgctcgggca cccgaacacc tggttggacc tggtcaggcc
    25861 aggaatcatg gcctacggcg cctatcccga cccggcggct gagcggacgg tcccgctgct
    25921 ccctggcctg gaatggacga gccgagtgac cttcgtgaag ccgatcagag ctggcgagac
    25981 cgtgagctat ggccgcacct ggacggccga gcgggacacc tggatcgcga ccgtgccggt
    26041 gggctacggc gacggctact cgcgcctgct gtcgaatcgc ggccgcatgc tgatcggtgg
    26101 ccgcagctac ccgatcgccg gccgggtgtg catggaccag accatgctcg atctcggccc
    26161 cgagaccgac gtcgcggtgg gcgatgaggt ggtgctgatc ggacgctccg gtgacgaaga
    26221 gatcactacc gcggagatcg ccgagctcat gggcacgatc ccctacgagg tcacctgcct
    26281 gatcacccag cgagtggccc gcgcctacgc ctagccaaaa gctaggggga agctcggggg
    26341 acggtttctt tccgcgtcac aacccttcgc tgacaaggtc aattgccagc cggggacggt
    26401 tcctttgtgt cgcgagcgct tccgccgagg ggatcccggc cttcgccggg atgacgttgt
    26461 aacggcggga tgacgttgga acagcaggat gacgttggaa cagcaggatg acgttggagg
    26521 agtgttggct actgccgcgg aatcgaggcc tggtgcctgg ccacgaactc gcgcttggcg
    26581 taggcggcca agccgaaggt gagccagccg tccacagcgg cgccgacgcc acctccgacc
    26641 agtggaatcc gccgcaccac cacgacggcg aggtgcttgc cgcccagacg tccggccagt
    26701 gaacccatca cgcgttcgtt gatcgcctgg tcgagcgacg ggtcgaacat cggtgcggtg
    26761 gcgatggcca gcggggtggc gggcagcctt ccgtccgcca ccatccggcg cacctcgtcg
    26821 tcgcccagca tggccaaggt gagggcggcg cgaacccggc gatcgtccac gtcatagccg
    26881 cgaaggtgcg cgatcgaagc gatcatccgg acgcttagca cgcccatgcc ggcgatgttg
    26941 gccggcagtc cgaccggcag ggttagcagg ccgccgacgc tggtcaggaa tccctgcgcc
    27001 gaagccagac cgatgtgttg ggtgaccagc gagtcgatcg cggcttcgcg ctcccccttg
    27061 gtctgcagcg ctttggctgc ggtcgctttc gccccgggga agctgctgtt cccgtcgatt
    27121 gcgaactcga cgatgctgcg cagcatgcca ccgaccacgg ctggcgcgcg ggccacgacc
    27181 tgcttgccga tcccttcgtt ggtcattggg ttcctttcac gccgaccctg caagcgtagg
    27241 cggcttcccg tggtggctgc gtccggtgcg tccctgaacc gtccctcaac acccatgtcg
    27301 tgatttcgaa gcttcagggg ctgcggacgc gaatctgggc accccgggca gtcatggctt
    27361 cgaaatcacg acaccggttg tcggaggcgg ctggcagaat cggcccatgg cgcgctcagg
    27421 actgttcgga ccggcgcggg agtggcccga ccaggatctg atcgccttct cggacgagtt
    27481 cgacgaaaca ctcgccctgg aggcgtacag ctgcggcgtg ttcccgatgc ccatcggcca
    27541 gtactccgcc agcgagatgg gttggtggtc gccgctgcgg cgcggggtga tgcccctgga
    27601 cgcccttcgg gtcacccgat cgctgcgcaa gtcggccaag cgctacacca ccacgatcga
    27661 tcaggccttc gatgaggtgc tggcgcggtg cgccgatccg aagcgtcccc acggctggat
    27721 cgacgatgcc gtccggcgcg tgtacaccac gctgcacgaa gccgggcggg tgcactcggt
    27781 tgaggtctgg gactcgtccg gacgcctcgt cggtggcctc tacggcgtct gcattggtgg
    27841 cctgttcgcc ggcgagtcga tgttccacga ccccgagcac gggcgggacg cgtccaaggt
    27901 ggcactgctc cgcttggtgg agttcctctc cgatgagcat gctgaggacc ggatcatcga
    27961 cgtccagtgg cagaccgaac acctggcctc gctggggata atcgaaatcg accgcgacga
    28021 gtacctgaac ctgctcgacg aagcgctggc tgtgcccgag ccgcactggc cggccagaga
    28081 ggagcgccat gcctgaactg ccggaagtgg aagctctgcg cgggtttctt gagcgcacct
    28141 gcgttggcag gacggtcgca cgcgtcgaac tggccgcctt ctcaagcctg aagacctaca
    28201 ccatcccgtt gtcggcgctc gccgggctcg aagtggacgc ggtcgagcgc cgcggaaagt
    28261 tcgtctgcct ctcgatcggc gggctgtggc tggcattcca cctggccagg gccggctggt
    28321 tgacctggtc ggacgcgatg ccggacgcac ccgcacgccc gggacgcggg ccgctggcgg
    28381 cgcgaatgat cttcgacgac ggctccgggt tccggctcac cgaggcgggc acgcagaagc
    28441 ggctggcgct ctacctggtg aacaacctgg acgaggtgcc cggcatcgcc agtctggggc
    28501 ccgacccgct ggcggcggag ttcacccccg aggtgctcgc cgggattctg gccacggccg
    28561 gacgcgcgca gctgaagggc gtgctccgtg accagcggat catcgctggc attggcaacg
    28621 cttactccga tgaaattctg cacgctgcga agttgagtcc gttcaagccg tcgaacggct
    28681 tggacgaggt cgaactggct gccctctacg ccgcgatccg cgacgagcta tcggctgcga
    28741 tccagcgttc cgcaggactg gcggccaagg atctcaaggg cgacaagaag ctcaacctgc
    28801 gggtgcacgg ccgcaccggc cagccctgcg gtgtctgcgg caccacgatt gccgaggtct
    28861 ccttcgccga cagttcgctg cagtactgcc ccggctgcca gactggcggc aaacccttgg
    28921 ctgatcggcg gttgtcacgc ttgctgaaat aggcggcccg ccgtcttcgt caatcaaccg
    28981 cgccgggtat cccgccaccc taggcgtgag cttgccgtcc gcttcgtaac ggtccggt
//
LOCUS       DAOPRQ010000029        28715 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_1292_length_28715_cov_10.056001, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000029 DAOPRQ010000000
VERSION     DAOPRQ010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 28715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28715
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1292_length_28715_cov_10.056001"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="PKJ61_05465"
     CDS             <1..374
                     /locus_tag="PKJ61_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016669940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HOC13304.1"
                     /translation="VFRAVKAATPGAYTFILRATREAPKVMLHPKKKTVGVRVPAHTT
                     ALALLQALGEPLMSSTLMLPGEEHPMTDGWQVNDAIGNQLDAVLDSGDCGLDPTTIVD
                     LSEDEPEVLRYGAGDPAPFEE"
     gene            complement(505..1542)
                     /locus_tag="PKJ61_05470"
     CDS             complement(505..1542)
                     /locus_tag="PKJ61_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012918725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOC13305.1"
                     /translation="MTRKRAARATKPRLLDIADLAKVSTATVSRVLNGKPGVGDEVRR
                     SVIAAMDILGYERPTAIKEKARGLIGVVVPELMNPIFPAFAQGIEGRLTQLGFTALLC
                     TQFPGGTTEDEAIDLLLDHGVSGIIFVSGRHADSSSSLQRYHDLRARGIPLAFINGWA
                     DGVDGTFVATDDTAAMDVVVRHLAALGHTRIGLAIGPERLVPARTKIAAFSAALRDHL
                     GETKPEKHVSKSLYTLEGGHAAAAQLIASGHTAIICGSDLMALGAIRCARGLGLSVPD
                     EVSIVGYDDSPLVAFVDPPLTTVHQPVGAMCQAVVSAVLGELDGNPGPRGELLFRPEL
                     IVRSSTHAAPK"
     gene            1739..2959
                     /locus_tag="PKJ61_05475"
     CDS             1739..2959
                     /locus_tag="PKJ61_05475"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter substrate-binding
                     protein"
                     /protein_id="HOC13306.1"
                     /translation="MRALSACASGPSATTAPATTAPTTTASATESTAADYSGATLTVW
                     VDDTRLSATQAAAKAFETATKAKVTLVKKNFADIQADFTSQVPTGKGPDITIGAHDWL
                     GNLIQNGVVAPIELGDKAADFENVALQAFTNEGQVYGVPYAIENIGLIRNTKLAPEAP
                     ASWDDALAAGKAAGTKYPFLIQVGDTGDPYTMYPLQASFGAPVFTQNEDGSYSSELAM
                     GGENGEKFAQWLADQGKAGTLDTAITYDIAVDAFAKGKSPFIIGGPWMLEKFKDLDLS
                     IDAIPSAGGQPASPFVGVQGFFISAQSKNAIVANDFLTNYIATTDVQYAMYEAGQRTP
                     ALKTAAEKAASDTLTAGFAKVAANAVPMPSIPEMAAVWTYWGVTEANIISGKQKPAAG
                     WQKMIADIQSKIKK"
     gene            3029..4618
                     /locus_tag="PKJ61_05480"
     CDS             3029..4618
                     /locus_tag="PKJ61_05480"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOC13307.1"
                     /translation="MGDLQQSVLTGKESAWNSRVFGVGFVVKLVLMALVNALGVYVIW
                     AAWNASSWVLMGAMVVIIAALDYVYFSNKHTLPGKYVAPGLVFLLIFQIFVIFYTGYV
                     SLTNYGDGHNLDKEQAVAAILAQNEKRVPGSASYPLTVVYTGDQLGFAIVDNGQVKVG
                     TADEPLRVAADATLAGNKVTAIPGFSIASSQQIFANQQAVTSLRVPFSANATEGSIRT
                     QNGTIGYLYKSIFAYDAAAGTMTDTENNVVYTASDRGQFVAPDGTALPTGWRVLVGFD
                     NFTKAFTDTQYSGPFLRVMLWTFAFALLSVLTTFLLGLFLAIVLNEERLRGRKLYRTL
                     LLLPYAFPGFLSALIWSGLLNEKFGFINQVLLGGTNIPWLTDPWLAKLSVLGVNLWLG
                     FPYMFLIATGALQALPNDIKEAAKVDGAGPLRTWLSVTGPLVLITTAPMLIASFAFNF
                     NNFSLIYMLTQGGPRFGDTSAPIGATDLLITMAYSVSGVDGNAPKNYGLASALSIMIF
                     VLIAVISGLGFRQTRKLEEVL"
     gene            4618..5520
                     /locus_tag="PKJ61_05485"
     CDS             4618..5520
                     /locus_tag="PKJ61_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004808745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOC13308.1"
                     /translation="MTAIQTVETMPDALPGERRKTPKQWLSQLGWRHLIGLIAVVYAA
                     FPIVYVMSASVASGGTLTGSNELFATVSWTNYVTLNSTRFWAWMGNSLLIASITAVGT
                     VIMGASAAYAFSRFRFTGRRAGLTTLLIIQMFPQMLAFVALFLLLLTFGEVVPALGLD
                     SRLSLICIYLGGALGANTFLMYGFFNSVPKEIDEAAKIDGATHAQTYWKIILPLVIPI
                     LAVVGLLSFIATFGEFIIARVVLQSEENWTLAVGLFGWVSSQLDANWGQFAAGAVLAA
                     IPVLALFLFLQKYIVSGLTAGAVK"
     gene            complement(5591..7321)
                     /locus_tag="PKJ61_05490"
     CDS             complement(5591..7321)
                     /locus_tag="PKJ61_05490"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase/ligase"
                     /protein_id="HOC13309.1"
                     /translation="MRTAPDSAAFEVRQGPGTELWRSVSATEFHGAVRRLAKGLIAAG
                     VRSGDRVAILADTRYEWAVADFACWFAGAVVVPIYPGSAPSQVAGIVTDAGVRLGLAG
                     TGEQRDLLQAALAAGTLRSWSMDAAETGDLAALAALGAHVSDEELEVRRQHATPDSVA
                     TIVYTSGATGEAKGAQITHGNLLHQVLNVAAAYTEVIREGGATVIFLPLAHVLARALQ
                     LVCLANGMRIAHLSDPREVVPALARLRPTFLVVVPRVLQKIRAAVAHRAARLGPLWRD
                     AERTAISWGRHLEAAQTSATRPPVALRLRHSGYERLFYRRIRTLFGGQLDYLLSGAAA
                     LDPELCRFFRGVGLPVLEGYGLTETCAPLTGNRPGSIRAGWVGTPLPGTTVRIGADGE
                     VLAKGSGVFIGYHGQDTVFPDGFFHTGDVGELDAEGRLRILGRLKEVIVTSTGKTVAP
                     AVWEQAMEQQPLVAHAVMVGESRPFLGGLVLLDRESLQAWAEREGVPELVQLPAAGEL
                     IELTHARLRRAVGSIVEVANRRVAKTEQARRFAVLLAGDSLVTPTLKLKRTRLIAQAD
                     AIIERLYQRD"
     gene            7526..8407
                     /locus_tag="PKJ61_05495"
     CDS             7526..8407
                     /locus_tag="PKJ61_05495"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HOC13310.1"
                     /translation="MNADLKSTITSLAGIAAAVLLGVGLAAAGSQHGVRVGGVPAFAI
                     AVVLAFVVQWLAFVPAYVLQTERFYDLMGSLTFITVTGMLVAVTPGVDARGLLAAALV
                     VIWAVRLGSFLFARVSRAGADDRFAEIKVSLPRFLLVWTMQGLWVSSTAAAAWIAISS
                     INRVALDAFALVGLLIWAVGFGIEVAADRQKSRFRADPGNRGRFIASGLWSRSRHPNY
                     FGEIVLWLGMALLALPTFTGWQWVGLISPVFVTVLLTRISGIPLLEVRADAKWGGQSD
                     YEDYKRRTPVLIPRLGR"
     gene            8426..8908
                     /locus_tag="PKJ61_05500"
     CDS             8426..8908
                     /locus_tag="PKJ61_05500"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOC13311.1"
                     /translation="MFAPYSPGRGEQPIQVAVVDLATEQIARCVEIVLQREGGHSDAW
                     RSRLEKAPTAPDQVTLVALVETQVVGYGSARRLTPRTLKPDAAVPDGWYLTGVIVDRP
                     SRRRGVASRLTEARLRWLSGRTGRVWYFVSDRIRPPSIRTPGLGSDWWPGISGFPASA
                     "
     gene            complement(8947..9576)
                     /locus_tag="PKJ61_05505"
     CDS             complement(8947..9576)
                     /locus_tag="PKJ61_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13312.1"
                     /translation="MADPAVVSQALADAWRSLLPEALGLGTELLERWSEPHRNYHALP
                     HLAHALDSLRRVGGGSPTEQLAVWFHDAVYTGTPGTDEEASAELAAARLPLSGLPPAA
                     VDEVVRLILVTIDHSPASGDLPGARVSDADFAILGSTSPRYLASVAALRAESPRLDAQ
                     FWRAGRRERVVELLGRDFLFYTDFGRARWEPLARANLNVELTALADSVR"
     gene            complement(9555..9992)
                     /locus_tag="PKJ61_05510"
     CDS             complement(9555..9992)
                     /locus_tag="PKJ61_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002515781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13313.1"
                     /translation="MSPILAITLKALVVIAIGLVAVLVGSPVVTAVFRRADPPPEVTG
                     SGGDQEPADPDADAPGIRSAASTLRGGRWIGMLERLAVYAAILTGFPEGIAVVLAIKG
                     LARYPELKATSSGAAERFIIGTFASVLFAAACAGLAWWLIRLW"
     gene            complement(9989..10639)
                     /locus_tag="PKJ61_05515"
     CDS             complement(9989..10639)
                     /locus_tag="PKJ61_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOC13314.1"
                     /translation="MKSNAFMFALVADQVSSRLGPDRVPLALAALSPTAMALPFERTA
                     GDELQGLCNEPAAVTTAVSQLTRLGDWRIGIGVGPVASPLPDSTRAARGPAYVAARTA
                     IERARRSPVQLALALTEHVSAADYPELTDATTDAETALWLWRSTLSRRSTEGWELMDL
                     LDQGLTKADAAAQLGISPSAVSQRLGRAAAVEDARGAALATRLLARVQELGGTVQP"
     gene            complement(10684..10887)
                     /locus_tag="PKJ61_05520"
     CDS             complement(10684..10887)
                     /locus_tag="PKJ61_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13315.1"
                     /translation="MNRLFAWFDRLIEADQRYACGVCDDRFNDLALAADHVLHCHPEF
                     RAVALYDGTEAEPISAAQPAFSR"
     gene            complement(11074..11499)
                     /locus_tag="PKJ61_05525"
     CDS             complement(11074..11499)
                     /locus_tag="PKJ61_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009375644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HOC13316.1"
                     /translation="MVEPKTKPTDADVGAFLEAATPAKRRADGLTLAEIFGAVTGVEP
                     LLWGPTMIGYGTYRYVSPVNPRNRGDWPKTGFSPRKAQLSLYGLKDLPAGAELLPKLG
                     KYTEGAGCVYVRRLEDIDLDILRELIAIAWSRADDPDPV"
     gene            complement(11704..13101)
                     /gene="lpdA"
                     /locus_tag="PKJ61_05530"
     CDS             complement(11704..13101)
                     /gene="lpdA"
                     /locus_tag="PKJ61_05530"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015582649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HOC13317.1"
                     /translation="MSQHFDVVILGAGPGGYVAAIRAAQLGKSVAVIEKQWWGGVCLN
                     VGCIPTKALLRNAELAHIFNREAKTFGIEGEVSFNYGVAFDRSRAVSDRMVKGVHFLM
                     RKNKITEINGWGTFVDAKTIKVATDAGEDTITFDSCIIAVGATTKLLPNTTRSARIFT
                     YEEQILSNQLPESIVICGAGAIGTEFAYVLANYGVQVTIVEYFDRMVPNEDPEVAAEL
                     LKAYKKLGVTVLTSTKVDSIEEWAEGVRVHVSPAAGGEGKVLEAERVLQSLGFQPRTT
                     GYGLENTGVALTERGAIAIDDFMRTNVDGIYAIGDVTAKLMLAHTAEAQGVVAAETIA
                     GAETMAIDYDMIPRATYCQPQIGSFGYTEAQAKDRGFEVKVAKFPFSANGKAHGLGDA
                     TGFVKIVADARYNELLGAHMVGPDVSELLPELTLAQQWDLTADEVSRNIHAHPSLSEA
                     IKEAVEGIAGHMINL"
     gene            13210..14736
                     /locus_tag="PKJ61_05535"
     CDS             13210..14736
                     /locus_tag="PKJ61_05535"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020385969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOC13318.1"
                     /translation="MKDLLPSSLGMLIRDARKQRGLTQQELADVLGTSQSAVHRIESG
                     GQNLSLEMINRIATALDSPLITAGSGGPTHLRIQGPVTLSGEIAVRSSKNAAVALLCA
                     ALINHGRTVLHGIAQIEEVNRILEVLTSIGVRATWSEDKSELVLVRPAKLDLADMDVA
                     AARRTRSIIMFLGPLLHSEDTFDLPYAGGCSLGARTITPHLQALRHFGLSVRTTQGLY
                     HADVDQACTGDRRITLTERGDTVTENVLMAAAQFPGRTEIRNASSNYMVQDLCFFLRE
                     LGVQVDGIGTTTLTVHGLDRIDADVAYTPSEDPIEAMSLITAAIVTGSELTVQRCPIE
                     FLDIELAILAEMGLNYSLTEEYAAYNGQTRLVDVTVKPSRLHSSADKIHPMPFPGLNI
                     DNLPFFAVIAAAAEGRTLIHDWVYENRAIHLIELNKLGADVQLLDPHRIAVNGPTRWR
                     GQELVCPQALRPAVCLLLAALAARGTSVLRDVYVINRGYEDLPNRLNALGAKIEVFHD
                     "
     gene            complement(14844..15698)
                     /gene="pdxY"
                     /locus_tag="PKJ61_05540"
     CDS             complement(14844..15698)
                     /gene="pdxY"
                     /locus_tag="PKJ61_05540"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase PdxY"
                     /protein_id="HOC13319.1"
                     /translation="MTTILSIQSSVAYGHVGNSAATFPLMRLGVEVWPVITVHFSNHT
                     GYGAWRGPLLAPGDVADVVAGIADRGVLGRVDAILSGYQGAAEMGAEILQAVRLVRKE
                     NPAAIYCCDPVIGDIGRGIYVRPGIPEFMRDQVVPAADVVTPNHFELDFLTGRTTTTL
                     AEVLGAARELQQRGPKVVLVTSTVLEGDHPDEINMLVVSADGAWQVTTPRLERNFTGS
                     GDLTAALFLAHLLNSETPGHAVGATADAVYSLLRRTAELESAELALVAAQQELVDPSH
                     HFEVRQLA"
     gene            15768..16550
                     /locus_tag="PKJ61_05545"
     CDS             15768..16550
                     /locus_tag="PKJ61_05545"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HOC13320.1"
                     /translation="MNVGDFPFLDHRFVAIAHRGGWTRPADAARENTVYAFERAVDLG
                     FSFLEIDVRTTADGKLVLFHDARLERLTGASGRVRERTWRQLRDLRIGGFDPVPLLED
                     VLDRFPATVFNIDLKDNAGVPALVALLRRMDVSDRIVVASFSTLRIRRFASRSPGIAL
                     GATPLGVGWDGFVPGLRRWRVDPAPVLQVPARVLGNLLPLVRRDLIDFLHSTGRKVHV
                     WTIDDRAEMERLIDLGVDGLISNDLVTLKSVLTERGLWEADE"
     gene            16547..17884
                     /locus_tag="PKJ61_05550"
     CDS             16547..17884
                     /locus_tag="PKJ61_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOC13321.1"
                     /translation="MRSTPAQRSWYWYDWANSAYITTTATVIIGPYLTSLANNAGCPQ
                     LAEGEICTNPVLLLGIFPVLPGALHPFLGTLSTIVSAILLLFVGAAADRSTKPHRWLA
                     TTAWMGALAGSLMFFLEGDNWQLGAWLMIIAAFCFGASTVVYDSILVRIAGPDERDKV
                     SSRGWAFGYLGGGLLLLANFALMSLHEQLGISYGLAVRLSLLSAGLWWGLFTIIPVFG
                     LRRLPREVSTPVARRNPFSQLMATFASMRNYPQTMLFLLAFLFFNDGIQTVIVSSSLY
                     ASSELGMATSQVMMTFLVTQFVAFGGALLFGRVAARIGAKRTVLGGIAIWLAVVLSAF
                     LLPAGNFGALIGLGAAIGLVMGGTQALSRSMYSQLIPLGREAEYFSFYQAMERGTSWL
                     GTLTFALVFQFSHSYRLALVALVVFFVVGGVLLTRVDMRKGITDAGNALPKVI"
     gene            17943..18278
                     /locus_tag="PKJ61_05555"
     CDS             17943..18278
                     /locus_tag="PKJ61_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein RbpA"
                     /protein_id="HOC13322.1"
                     /translation="MADRALRGFGLGAKSHADPSGVELAPRQEIGFECPKGHQFNVVF
                     AEEAELPTEWECPRCGATSRRSDGVEPEAKETKAPRTHWDMLRERRSIEELEDLLAER
                     LEEIRGDGQ"
     gene            complement(18360..18908)
                     /locus_tag="PKJ61_05560"
     CDS             complement(18360..18908)
                     /locus_tag="PKJ61_05560"
                     /inference="COORDINATES: protein motif:HMM:NF016102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HOC13323.1"
                     /translation="MNRNLFAGRWVLPALVVGFILVAVAEVWLLTFVGSRIGALPTLG
                     ILLAEALVGAWLLRREGSKAWTALTQAINTGRMPTGQLADAALVLIGGIMLMLPGFFT
                     DFIGVFFLLPLTRPLVRKSLGFLIARQAAKSGVDFGVIKAKYGPGTVIRGETADDVPS
                     PATDAGTSVVIEGEIEPGGRPA"
     gene            complement(19028..19765)
                     /locus_tag="PKJ61_05565"
                     /pseudo
     CDS             complement(19028..19765)
                     /locus_tag="PKJ61_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002545252.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
     gene            complement(19743..21428)
                     /gene="lnt"
                     /locus_tag="PKJ61_05570"
     CDS             complement(19743..21428)
                     /gene="lnt"
                     /locus_tag="PKJ61_05570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HOC13324.1"
                     /translation="MFADRPGQPGLDELDQPVGSASATRAPTFSHWLANRHWGVRAAL
                     AILAGVLTALGFEPFGAWPLVIPGVAGLTLAVLAARRLRGAAGLGFAFGVGFLLLGVG
                     WMQVIFLQAMLGLVLVEALFYAVLGAAIKLASRTAWWPLLAAACWTGIEFAYSRFPFS
                     GFGWMRLGYAMVDSPLAAMLPLVGVATLGFSTAMAGQLLAWLVLSPSKQRTALVASGV
                     AGIAVLALGGLLVPAGLQTGTTSVGYVQGGAPGGGVYGLGEARTITRNQAAETARFAD
                     QMSETDRLADDVDTGTRPPPAFIVWPENGTDMDPFLDAETNRLVQAALDRAGVPILVG
                     AILEGPGKDERRTASLWWDPEAGVQGTYIKRGIVPFGEWVPFRSILLPLIPELQYVGA
                     QSIPGTAPGVLPVTLADGTALKLGILVCYDLVYDDFAYDTVRAGGQVQIVQSSNAMYQ
                     GTGQIEQQFAITRVRARELRREMLVVTTSGVSGLINPDGSVAFTEPDHVSASGVVELP
                     TREGVTPAAWGASWIELTITLLAVVGLGLSLRYGRMDVEVTQLGEANGLPGRS"
     gene            21482..22399
                     /locus_tag="PKJ61_05575"
     CDS             21482..22399
                     /locus_tag="PKJ61_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease"
                     /protein_id="HOC13325.1"
                     /translation="MADRLLAFDTASLYFRAFFGLPSTLRAADGRPVNAVRGLLDFLA
                     RFITDYSPTQVACGWDNNWRPTWRVDLVPSYKAHRVAYGVVEEVPADLVGQVPLILEV
                     LAALGLSVIGADDHEADDVLGTFATVAGVPTDVVTGDRDLFQLVDDEREVRVLYCGRG
                     VANHDRVDAAWLLAKYGIDGAGYVDFAVLRGDPSDGLPGVPGIGERTAARLVADYGDL
                     EGIVAAAVSGRLSPTLTARLKAAVDYLGAAREVVAVRRDLDLAPVDLTLRAAPADPVR
                     FAELAEELNLGGSAERILAAMNLRGAGPS"
     gene            complement(22451..>22828)
                     /locus_tag="PKJ61_05580"
     CDS             complement(22451..>22828)
                     /locus_tag="PKJ61_05580"
                     /inference="COORDINATES: protein motif:HMM:NF018696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HOC13326.1"
                     /translation="HSYDVVVLSRTIQTIHRPFEVLQEMGRIADRLIVSVPNFGYWRH
                     RLRLLRGRMPMSREIPHAWYDTPNLRHTTLVELEELFDRLGLTVERRIVLSPAGTPLP
                     SGNRFANVVAGSAVYALRSNHTD"
     assembly_gap    22829..22928
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<22929..23981)
                     /locus_tag="PKJ61_05585"
     CDS             complement(<22929..23981)
                     /locus_tag="PKJ61_05585"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016669902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HOC13327.1"
                     /translation="MAGSTQAGSVGLVNTQTVRLFGADVPLELAQGGTLGPVDVAYET
                     YGTLSPAADNAVFVCHALTGDAHAAGFHEGAKRPGWWDNLIGPGKPLDTDRFFVVSAN
                     LIGGCQGSTGPSSTNPATGSAYGLDFPVLMMSDLVNVHRALMRHLGISRLLAALGGSL
                     GGMQVLQWGLDHPDEIANALVIAASSRLTAQNIAFSAVARRAIMTDPNFADGRYAEAG
                     VNPDTGLSIARMMAHITYLSEDALTEKFGRKPHPSRSHPGFGVDFAVESYLDHQGQVF
                     LERFDALSYLYLTRVMDYFDAFASPDALAKISVHPVNWLVLSFASDWRFGTDHSRRIV
                     RNLEHAGQPVTFREIPS"
     gene            24081..24548
                     /locus_tag="PKJ61_05590"
     CDS             24081..24548
                     /locus_tag="PKJ61_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015070224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HOC13328.1"
                     /translation="MSELKDKINADKVAAMKAKDEMALSTLRMALAAIQTEEVAGKVA
                     RELSDADVLAVLNREVAKRRDSAEAYTTGNRPELAAKELAEVEVLQRYLPAALSDEEL
                     DAIVTAALAAAAAELGAAPTLRQMGQVIKAVNAEVAGRAEGAQVAAKVKAALA"
     gene            complement(24600..25874)
                     /locus_tag="PKJ61_05595"
     CDS             complement(24600..25874)
                     /locus_tag="PKJ61_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011758041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOC13329.1"
                     /translation="MAAVREVFADTTPLRNPHFRRLWQANIITVIGAQLTVVAVPAQI
                     YAMTQDSGYVGLTGLFGLVPLIVFGLWGGALADHFDRRRLLQVTTVGLIVTSGLFAVQ
                     SALGINNVWLLLGLFALQQAFFGVNQPARSAVLPRLLPEAQLPAANSLNMTVMMAGAI
                     AGPMIGGALIPVLGYAWLYLIDTVFLFATLRAVWLLPSLSVENRTGSPGLKSVVDGIS
                     YLKGHPILLMSFVVDLIAMIFGMPRALAPQIAHQSFGGPIEGGTEFALLMAAMPLGAV
                     LGGVLSGWISKVTRQGRAVVVSILIWGAAMALLGLAVGLAGAWRTPMLVAALVCLAVG
                     GAADMASAAFRQAMLQSAADDAVRGRLQGVFIVVVAGGPRIADVLHGYAAGGIGTAWA
                     TGGGGLLVILFTILVTMAVPAFWRYVPKSVSG"
     gene            complement(25896..26450)
                     /gene="thpR"
                     /locus_tag="PKJ61_05600"
     CDS             complement(25896..26450)
                     /gene="thpR"
                     /locus_tag="PKJ61_05600"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HOC13330.1"
                     /translation="MAERLFVAVLPSASVREALDTFLEPRRTNALNLRWTLPESWHLT
                     CAFMADVHPSAVPDLEAALAEVAARTRPFAVTIEGAGAFPDPDHARAFWLGVSAGGTE
                     LAQLAKRSRTAVTRSGVEVEGGRLRPHLTIARANGISGTGWLEVFNTIPAQTWAVERF
                     SLIRSQSLPGGAGYQSLAEFDLAN"
     gene            complement(26443..27030)
                     /locus_tag="PKJ61_05605"
     CDS             complement(26443..27030)
                     /locus_tag="PKJ61_05605"
                     /inference="COORDINATES: protein motif:HMM:NF015674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HOC13331.1"
                     /translation="MSLIEPVSVAPEELVKRLWWLTLTRGIFAIALGLFALFVPGWTV
                     SALFMLFGVFTLADGIVALGIGIFAPRTVWGSSVFQGIAGIVIGLLVLRYPQTVAAIV
                     VVIFAIWALVIGLFQIVLAFRLRANGGHWLWVLVSGLVTAFLGIYFLVNPDTGVAVLA
                     ITIGIFALIAGAVLIFGAVQVRRFHDELKVLRVDG"
     gene            27112..28287
                     /locus_tag="PKJ61_05610"
     CDS             27112..28287
                     /locus_tag="PKJ61_05610"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOC13332.1"
                     /translation="MEPDQTAADASETELSTAMLAEPDATNPTPSGKRQLRVLADLGH
                     PFAWGFLAAAGLLVATSLGGALSALSTVAVSIGVALFIALALEPLVRWLGKHGMSRGM
                     SIAVVFVGFAVFLGGVLAIVIPMVAVQITQFAQAIPGYITNLQNAEWFQNLLAASGSS
                     DLYENLLNQARTWLSNPANLLTIGGGALAVGTGLINMISGTMIAIVLALYFLASINTI
                     KSAFTSLAPAYARPRLGKMTDHVTESVGQYVSGMAILALCNSIFAFILLSILGVPFAF
                     LMAFLALLITMIPMIGSVLFWLLASLVALLHAPLTGLIFLVVYFAYMQVEAYVMTPRV
                     MNKAVDIPGSLVLIGAMVGGTLLGLLGALIAVPVTATLLMILKEVFIPRQDAKVVPD"
     gene            28440..>28715
                     /locus_tag="PKJ61_05615"
     CDS             28440..>28715
                     /locus_tag="PKJ61_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13333.1"
                     /translation="MDERTGLVPVEVALARIRVALNSIDPDRSRLAPEARLRVATAAR
                     SLTGRLDALTSQLLAEADASHASERATGTPTSTWLAIDQNLTKREAAG"
BASE COUNT         4768 a         9390 c         9636 g         4821 t
ORIGIN      
        1 acgtgttccg cgcagtgaag gcggccacac cgggcgccta caccttcatc ctgcgggcca
       61 cccgagaagc gccgaaggtc atgctgcacc cgaagaagaa gaccgtcggg gtgcgcgtcc
      121 ccgcccacac caccgcgctg gcgctgttgc aggcgctggg ggagcccctg atgtcctcga
      181 cgctgatgct gccgggcgag gagcacccga tgaccgacgg ctggcaggtc aacgacgcga
      241 tcggcaacca gctcgatgcc gtgctcgatt cgggggactg cggcctcgac ccgaccacga
      301 tcgtcgacct cagcgaggac gagccggagg tcctgcgcta cggggccggt gacccggcgc
      361 ccttcgagga gtagggaagc gcttcaacca tccctgcgat tgacgaacgg tcgtccgcct
      421 gcgcgtccgc tcacccgccg cccatcgatc actgtggaat cacccaccac ggacggtggg
      481 catacccacg gactgaggct cgaactactt cggcgcggca tgcgtcgagc tgcgcacgat
      541 caactccgga cggaacagca actcgcctcg ggggccggga ttgccgtcca gttcgccgag
      601 cacagccgaa acgaccgcct ggcacatggc gcccaccggc tggtggaccg tggtcagcgg
      661 cgggtcgacg aaagccacga ggggggagtc gtcgtagccg acgatcgaga cttcatcggg
      721 cacggacagc cccaggcccc gagcgcaacg gattgcgccg agggccatca ggtcggaacc
      781 gcagatgatc gcggtgtgcc cggacgcaat cagttgcgcg gctgcggcgt ggccgccctc
      841 gagcgtatag agcgacttgc tgacgtgctt ctccggcttg gtttcgccga ggtggtcgcg
      901 cagcgcggca ctgaaggccg cgatcttggt ccgcgccggc accagccgtt ccggtccgat
      961 cgccagtccg atcctggtgt ggcccagcgc ggccaggtgc cgcaccacca cgtccatcgc
     1021 cgcggtgtcg tcggtcgcga cgaaggtgcc gtccacgccg tccgcccagc cgttgatgaa
     1081 ggccaacgga atcccgcggg cgcgcagatc gtggtagcgc tgcaggcttg acgagctgtc
     1141 cgcgtgtcgg ccagagacga agatgatgcc cgagacgcca tggtcgagga gcaggtcgat
     1201 ggcctcatcc tccgtagtcc cgccgggaaa ctgggtgcaa aggagggcgg tgaaccccag
     1261 ttgggtgagg cggccctcga tgccctgggc gaaagcgggg aagatcgggt tcatcagttc
     1321 gggcacgacc accccgatca gcccgcgcgc cttctccttg atcgcggtcg gtcgctcgta
     1381 gccgaggatg tccatcgcgg cgatcaccga tcgacggacc tcgtcgccca cgccgggctt
     1441 gccgttgagc acccgcgaca ccgtggcagt gctcaccttc gccaagtcag caatgtcgag
     1501 caggcgcggc ttggttgcgc gcgcggctcg ctttctggtc atggtcactc ctgtaacggc
     1561 gtcgtcgttg gcaaaagtta ccggtatctg tgcgcagatg ggggtttcaa ggtctcgatt
     1621 cggtaacgat acgtaacgcg ttgcgtaact ggtgcaagaa cgcactaacg ttcgttacgt
     1681 ccaaagggcc gtcccgcagc cgggacgagc atcccagtgc agacaggaag aggagcagat
     1741 gcgcgcgctg agcgcgtgcg cgtccggccc gtccgccacg accgccccgg ccaccacggc
     1801 cccgaccact accgcgagtg ccaccgagag caccgcagcc gactacagcg gcgccacgct
     1861 gaccgtatgg gtggacgaca cccggctttc tgcaacgcag gccgcagcca aggctttcga
     1921 gaccgcgacc aaggcgaagg tgaccctggt caagaagaac ttcgccgaca tccaggccga
     1981 cttcacctcc caggtgccca ccggtaaggg ccccgacatc accatcggtg cgcacgactg
     2041 gctgggcaac ctgatccaga atggcgtcgt cgccccgatc gagctcggcg acaaggcggc
     2101 cgacttcgag aacgtcgcgc tgcaggcctt caccaacgag ggccaggtct acggcgtccc
     2161 gtacgcgatc gagaacatcg gcctgatccg caacaccaag ttggcaccgg aagctcccgc
     2221 gagctgggac gacgcgctgg ccgcaggcaa ggccgccggc accaagtacc cgttcctgat
     2281 ccaggtcggc gacaccggtg acccgtacac catgtacccg ctgcaggcct ccttcggcgc
     2341 accggtgttc acccagaacg aggatggctc ctacagcagc gagctggcca tgggtggcga
     2401 gaacggtgag aagttcgcgc agtggctggc cgaccagggc aaggccggaa cgctcgacac
     2461 cgccatcacc tacgacatcg cggtggacgc gttcgccaag ggcaagtcgc cgttcatcat
     2521 cggcggcccg tggatgctgg agaagttcaa ggatctcgac ctctccatcg acgcgatccc
     2581 gtccgccggt gggcagcccg ccagcccgtt cgtcggcgtc cagggtttct tcatctccgc
     2641 ccagtccaag aacgccatcg tggccaacga cttcctgacc aactacatcg ccaccactga
     2701 tgtgcagtac gcgatgtacg aggccgggca gcgcaccccc gcactgaaga ccgcagccga
     2761 gaaggccgcc agcgacacgc tgaccgccgg cttcgccaag gtcgccgcga acgcggtgcc
     2821 gatgccgtcc atccccgaga tggccgccgt gtggacctac tggggtgtca ccgaggcgaa
     2881 catcatctcc ggcaagcaga agccggccgc cggatggcag aagatgatcg ccgacatcca
     2941 gtcgaagatc aagaagtagt ctgcagccag cggggacgtc gatgacagca tcggcgtcca
     3001 cgctggcgca ccagcgcgaa gggcgatcat gggcgatctg caacagtcgg tgctgaccgg
     3061 caaagagagt gcgtggaact cacgggtgtt cggcgtgggc ttcgtggtga aactggtcct
     3121 gatggcgttg gtcaacgcct tgggcgttta cgtcatctgg gcggcgtgga acgcttcgtc
     3181 ctgggtgctg atgggcgcga tggtggtgat catcgccgct ctcgactacg tctacttcag
     3241 caacaaacac acgcttccgg gcaagtacgt agcgcctggt ctggtgttcc tgctgatctt
     3301 ccagatcttc gtgatcttct acaccggcta cgtctcgctg accaactacg gcgacggcca
     3361 caaccttgac aaggagcagg cggtcgcggc gatcctcgcc cagaacgaga agcgcgtccc
     3421 tggctcggct tcctacccgc tcaccgtggt ctacaccggc gatcaactcg gtttcgcgat
     3481 cgtcgacaac ggccaggtga aagtcggtac cgcggacgag ccgctacgcg tcgctgcgga
     3541 cgcgacgctg gccggcaaca aggtcaccgc gattccgggc ttcagtatcg ccagctcaca
     3601 gcagatcttc gccaaccagc aggctgtgac cagcctccgg gtgccattca gcgcgaacgc
     3661 gaccgagggc tcgatccgca cccagaacgg cacgatcggt tacctctaca agtcgatctt
     3721 cgcctacgac gcagccgccg gcacgatgac cgacaccgag aacaacgtgg tctacaccgc
     3781 gtccgaccgg ggccagttcg tcgcacctga cgggaccgca ctgcccaccg gctggcgggt
     3841 cctggtgggc ttcgacaact tcaccaaggc cttcaccgat acgcagtact ccggcccgtt
     3901 cctgcgggtg atgctgtgga cgttcgcgtt cgccctgctg tcggtgctca ccaccttcct
     3961 gctgggactt ttcctcgcga tcgtgctcaa tgaggagcgg ctgcgcggac gcaagctcta
     4021 ccgcacgctg ttgctgttgc cgtatgcctt cccgggcttc ctctcggcgc tgatctggtc
     4081 gggcctgctg aacgaaaagt tcggcttcat caaccaggtg ctgctgggcg gcacgaacat
     4141 cccgtggctg accgacccct ggctggcgaa gctgtcggtg ttgggcgtga acctttggct
     4201 gggcttcccc tacatgttcc tgatcgccac cggggcgctg caggcgctgc ccaatgacat
     4261 caaggaagcc gcgaaggtgg acggtgccgg cccgctgcgc acctggctat cggtcaccgg
     4321 cccgctggtg ttgatcacca ctgcgcccat gctgatcgcg agcttcgcct tcaacttcaa
     4381 caacttctcc ctgatctaca tgctcaccca gggcgggccg cgattcggcg acacgtccgc
     4441 accgatcggg gccaccgacc tgctgatcac catggcgtac tcggtctccg gcgtggacgg
     4501 caatgcaccg aagaactacg gcctggccag cgccttgtcg atcatgatct tcgtcctgat
     4561 cgcggtgatc tccggtctcg gcttcaggca gacccgcaag ctcgaggagg tgctgtgatg
     4621 actgccatcc agacggtcga aaccatgccg gacgctctgc cgggtgagcg tcgaaagacc
     4681 cccaagcagt ggctcagcca gctcggctgg cggcatctga tcggcttgat cgccgtggtg
     4741 tacgccgcgt tcccgatcgt ctacgtgatg tcggcatcgg tcgcttccgg tggcacgctg
     4801 accggctcga acgagttgtt cgccaccgtg tcctggacga actacgtcac gctaaacagc
     4861 acccggttct gggcctggat ggggaactcg ctgctgatcg cgtcgatcac cgcggtgggc
     4921 accgtgatca tgggcgcgag cgcggcctac gccttctcgc ggttccggtt caccggtcgt
     4981 cgggccgggc tcaccacgct gctgatcatc cagatgttcc cgcagatgct ggctttcgtg
     5041 gcgctgttcc tgctgctgct caccttcggt gaagtggtgc cggcgctcgg cctggactcg
     5101 cggctgtccc tgatctgcat ctacctcggt ggggcgctgg gtgcgaacac cttcctgatg
     5161 tacgggttct tcaactcggt gccgaaggag atcgacgagg cggcgaagat cgacggcgcc
     5221 acccacgccc agacctactg gaagatcatc ctgccgctgg tgatcccgat cctggccgtg
     5281 gtggggctgc tgagcttcat cgcgacgttc ggtgaattca tcatcgcccg ggtggtgctg
     5341 cagtccgagg agaactggac gctggcagtc ggactgttcg ggtgggtgtc gtcgcagttg
     5401 gacgccaact ggggccagtt cgctgccggc gctgtgctcg cagccattcc ggtgctggcg
     5461 ctgttcctct tcctgcagaa gtacatcgtt tccgggttga ccgcaggcgc ggtcaaatag
     5521 tttccattcg acgggggacc gtccggcccg gcagccgtag gtgaggcacg gctgccgggc
     5581 cggccgtaca tcaatcgcgt tgatacagcc gttcgatgat cgcgtccgcc tgcgcgatga
     5641 ggcgcgtccg cttgagcttc aaggttggcg tgacgaggga gtcgccggcg agcagcacgg
     5701 cgaaacggcg tgcctgctcc gtcttggcca cccgtctgtt cgccacctcg acgatgctgc
     5761 cgaccgccct ccgcaggcgg gcgtgagtga gctcgatcag ctcgccagcc gccggtagct
     5821 ggactagttc ggggacgcct tcgcgctcgg cccatgcctg cagcgactcc cggtcgagca
     5881 gcaccaggcc acccagaaag ggacgggatt ctccgaccat gacggcgtgg gcgaccagcg
     5941 gttgctgttc catggcctgt tcccaaaccg caggcgccac cgtcttgccg gtcgaggtga
     6001 cgatgacttc cttgaggcgg ccgagtattc gcagccggcc ttcggcgtcg agctcgccca
     6061 catcgccggt gtggaagaag ccatcaggga aaacggtgtc ctgtccgtgg tacccgatga
     6121 acacaccgct gcccttggcg agtacttccc cgtccgcccc gatcctgacg gtggtgccgg
     6181 ggaggggagt cccaacccag cctgctcgga tcgagcccgg tcggttcccg gtcagcggag
     6241 cacaggtctc ggtgaggcca tacccttcga ggaccggcag gccgacaccc ctgaagaacc
     6301 ggcaaagctc ggggtcgagt gcggctgccc cagagagcag gtagtcaagc tgcccaccga
     6361 agagcgtccg gatgcggcgg tagaagagcc gttcgtagcc cgagtgccgg agcctgagcg
     6421 cgactggcgg ccgggtggcg gaggtttgag cagcctcaag gtgacgcccc cagctaatcg
     6481 ccgtccgttc ggcgtcgcgc cagagcggac cgagtctggc ggctcggtgt gcgacagccg
     6541 cgcgtatctt ctgtagcacc cgcggcacga ccaccaagaa agtcggacgc aaccgcgcga
     6601 gcgccggcac cacctcgcga gggtcggaca ggtgagcgat gcgcatgcca ttcgctaggc
     6661 agaccagctg gagtgcccgt gcgagcacat gggccagtgg caggaagatg acggtggcgc
     6721 caccttcgcg gatcacctcg gtgtatgcgg cggcgacgtt gagaacttgg tgaagcaggt
     6781 tgccgtgcgt gatttgggcg cccttagctt ccccggtcgc accggaggtg tagacgatgg
     6841 tcgccacgct atccggagtg gcgtgctgtc gtctgacctc caactcctcg tcactcacgt
     6901 gagcgccaag ggcggcgagg gcggcgagat caccagtctc cgcagcgtcc atcgaccagc
     6961 ttcgcagcgt gcctgcggcc agcgctgcct ggagcaggtc gcgctgctcc ccggtgccgg
     7021 ccagacctag gcggacgccg gcgtcggtga ctatgccggc gacttgcgac ggggctgagc
     7081 ccgggtagat cggcaccacc accgcaccgg cgaaccaaca ggcgaaatcg gctaccgccc
     7141 actcgtagcg ggtgtcggcc aggatggcca cgcgatctcc tgacctgact ccggcagcga
     7201 tcagtccctt cgccagacgg cgtaccgcac catggaactc agtggcagag acggaccgcc
     7261 acagctccgt cccggggccc tgacgaactt cgaatgccgc agagtcgggt gctgtccgca
     7321 cccgttccag cagaagatcg gtggtgttgc ggcgccatgc cagctcagcc agaaccggcg
     7381 tgcttgcttc tcgcacgggc gcagcgcctc ctattgccaa ctcagatgcg cgtcaagact
     7441 gccaatgccg ggccgggtgg accaagccca ggcacaacct ttgacaagcg gaagccaagc
     7501 gagttccggt tcggttgagt cgggtgtgaa tgcagatctc aaatcaacga tcacctcttt
     7561 agccggcatc gcagcggcag tcttgttggg agtcgggctc gccgcagccg gcagccagca
     7621 cggcgttcga gttgggggcg tgccggcgtt cgcgatcgct gttgtcctcg ccttcgtcgt
     7681 gcagtggctg gcgtttgtgc ccgcctacgt cctgcagacc gagcgcttct atgacctgat
     7741 gggaagcctg acgttcatca cggtgaccgg gatgcttgtg gcggttacgc caggggtgga
     7801 tgcccgcggc ctcctcgccg ccgctttggt ggtgatctgg gcggttcgcc tcggcagctt
     7861 cctgttcgct cgcgtgtcac gcgctggcgc ggatgatcgg ttcgccgaaa tcaaggtctc
     7921 gttgcccaga ttcctgctgg tgtggacgat gcaaggtctg tgggtcagca gcaccgcggc
     7981 ggccgcctgg attgcgattt cttccatcaa tcgcgttgct ctggacgcgt ttgcgctggt
     8041 ggggttgctg atctgggcgg tcggcttcgg catcgaagtc gccgcggacc ggcagaagag
     8101 ccgtttccgg gcggacccgg gcaatcgagg acggttcatc gccagcggtc tttggtcgcg
     8161 atccagacac ccgaactact tcggcgagat cgtgctctgg ctcgggatgg cgctgctggc
     8221 gttgcccacc ttcaccggct ggcagtgggt tgggctgatc tctcccgtgt tcgtgacggt
     8281 tctgttgacc cggatcagcg ggattccgct gctggaagtc agggccgacg cgaagtgggg
     8341 cgggcagagt gactacgaag actacaagcg gcgaacgccg gtgctgatcc ccaggttggg
     8401 taggtagggt ctccaagtta gttccttgtt cgccccgtac tctcccggtc gcggcgaaca
     8461 acccatccag gtggcggtgg tcgacctcgc taccgagcag atcgcgcgat gcgtggagat
     8521 tgtgttgcag cgcgagggtg gacactccga cgcctggcgc agccggctcg agaaggcgcc
     8581 gaccgcgccg gatcaggtga cgttggtggc gctggtcgag acgcaggtgg tcggctatgg
     8641 ctcggcccga cgactcaccc cacgcacttt gaagcctgac gccgcggtgc cggacggttg
     8701 gtacctgacc ggggtgatcg tcgatcggcc ctctcgccgc cgcggggtcg cctcccggct
     8761 gaccgaggcg cggctgcgct ggctgagcgg acgtactggg cgggtgtggt acttcgtctc
     8821 cgacagaatc cggcctccat cgatccgcac gcccggtttg ggttcggact ggtggcccgg
     8881 gatatctggt ttcccggcgt cagcgtgact ggatcggccc tgctgtacgc ggtcgatctc
     8941 tccaggtcag cggacggaat ccgcgagcgc ggtcagttcg acgttgagat tggcgcgggc
     9001 cagaggttcc cacctggcgc gtccgaaatc ggtgtagaaa aggaaatccc ggccgagtag
     9061 ttcgacgacc cgctcgcgac gcccagctcg ccagaattgc gcgtcaaggc ggggcgactc
     9121 ggcgcgtagc gcagccaccg aggctagata acgtggcgac gttgagccca gaatggcgaa
     9181 atcggcgtcg ctgacgcggg caccgggcag atcgccggaa gcgggggagt ggtcgatcgt
     9241 gacgaggatg aggcggacga cctcgtccac agccgctggc gggagcccgg agagcggtag
     9301 tcgcgccgcg gccaactcgg ccgaagcttc ttcgtccgta cccggagtcc cggtgtacac
     9361 cgcgtcgtgg aaccagaccg cgagctgctc ggtcgggctg ccgccgccca cccggcgaag
     9421 cgagtcgagc gcatgcgcga gatgggggag tgcgtgatag ttgcggtgcg gctccgacca
     9481 gcgttcgagg agctcggtgc cgaggccgag ggcttccgga agcagcgagc gccaggcgtc
     9541 cgccagggcc tgactcacca cagccggatc agccaccagg cgagtccggc gcaggcggct
     9601 gcgaacagga cgctggcgaa ggtgccgatg atgaatcgtt cggcggctcc gctcgaggtc
     9661 gccttgagtt cggggtagcg ggcgaggccc ttgatcgcga gcaccacggc gatgccttcg
     9721 gggaagccgg tgaggatcgc ggcgtagacg gccaagcgtt ccagcatgcc gatccagcgg
     9781 ccgccgcgca gagtgctcgc ggccgaccga atgccgggtg cgtccgcgtc cgggtctgcg
     9841 ggctcctgat cgccgccaga accggtgacc tccggcggag gatccgcgcg gcggaagacg
     9901 gcggtgacca ccgggctgcc gacgagaacg gcgaccaagc cgatcgcgat caccacgagc
     9961 gccttcagcg tgatcgccag aatcgggctc atggctgcac cgttccgccg agttcctgca
    10021 cgcgcgccag gagccgggtg gcgagggcgg ctccccgagc gtcctcgacc gccgctgcgc
    10081 gaccgaggcg ttggctcacc gcagacgggc tgatgccgag ttgggcagca gcgtcggcct
    10141 tggtgaggcc ctggtcgagc agatccatca actcccatcc ttcggtgctg cggcgcgata
    10201 gcgtcgaccg ccacagccag agcgcggtct cggcgtccgt ggtggcgtcc gtcagttcag
    10261 ggtagtcagc cgcactgaca tgctcggtca gcgcgagggc gagttggacg ggggagcggc
    10321 gcgcccgctc gatggccgtt cgggcagcga cgtaggcggg cccacgagca gctcgcgtgg
    10381 agtcaggcag gggtgacgca actggtccga cgccgatgcc gatccgccag tcgcccagcc
    10441 gggtcagttg gctgaccgct gtcgtgacgg cggcgggctc gttgcagagt ccctgtagtt
    10501 cgtcacccgc ggtgcgctcg aacggtagcg ccattgctgt gggtgacagg gcagccagag
    10561 caagcggcac ccggtcggga ccgagacggc tggacacctg atcggcgacg agagcgaaca
    10621 tgaaggcgtt tgacttcatt cctcgatgtt aagccttcag ccttaagtgc gcaactgtct
    10681 ggctcagcgc gagaaggcgg gctgcgcggc cgagatcggt tcggcctcgg tgccgtcata
    10741 gagcgcgacc gcacggaact ctgggtggca gtgcaggacg tggtcggcag caagagcgag
    10801 atcattgaag cggtcgtcgc agacgccgca cgcatagcgc tggtcggcct cgatcaggcg
    10861 atcgaaccag gcgaacagcc ggttcatggg agctcctggg gggtggatcg ccgaccagcc
    10921 tagccgtagc gggcggccga tagtaacgac acggccaagt tgctggcgaa tgcgcgcgtg
    10981 gggcaggcgt gaggagtcca aagtgacata atgcaagtta tcggacacat gctgtggagg
    11041 ctgtggggtc gccataccgc ccgctctggt tgcctacacc ggatcggggt cgtccgcccg
    11101 cgaccaagca atcgcaatca gttcccgcaa gatgtccaga tcgatgtcct caagcctgcg
    11161 gacgtacacg cagccggcgc cttcggtgta cttgcccagt ttcggcagca actccgcacc
    11221 cgccggcaga tccttcaacc cgtacagcga caactgcgcc tttcgtggcg aaaacccggt
    11281 cttgggccaa tcgccgcgat tgcgtggatt cacgggcgag acgtagcggt aggtgccgta
    11341 gccgatcatc gtcggccccc acagcagcgg ctccactccg gtgaccgcac caaagatctc
    11401 ggcgagcgtc agcccgtcgg cgcggcgctt ggctggtgtt gccgcttcga ggaacgcgcc
    11461 cacgtcggcg tccgtcggtt tcgtcttggg ttcgaccatc gcgtgatcct aacggtcgtc
    11521 tggaagtcgc caccggtggc gggcgatcac ctggtcaagg ctgccgtcca gcctccagag
    11581 gcctggttga ctgtggcatc gcccaccgcg gacggtgggc tcagccacag tctgtggcgg
    11641 cggaaactgt tccgccgggt tggttccgca cccggcggtt cttcaggaac cgttggtggc
    11701 gggctagagg ttgatcatgt ggccggcgat gccctccacg gcctccttga tcgcttcgga
    11761 aaggctcgga tgcgcgtgga tgttgcggct gacctcgtcc gcggtgaggt cccactgctg
    11821 ggccagcgtc agttcaggca gcagctcgga gacgtccggg ccgaccatgt gcgcgccgag
    11881 cagctcgttg tagcgggcgt cggccacgat cttcacgaag ccggtggcgt cgccaaggcc
    11941 gtgcgccttg ccgttcgcgc tgaacgggaa cttggccacc ttgacctcga agccgcggtc
    12001 cttggcctgc gcctcggtgt agccgaacga accgatctgc ggctggcagt aggtcgcgcg
    12061 cgggatcatg tcgtagtcga tggccatagt ctcggcgccg gcgatcgtct cggccgcgac
    12121 cacgccctgc gcctccgcgg tgtgcgccag catcagcttc gcggtcacgt cgccgatcgc
    12181 gtagatgccg tccacgttcg tgcgcatgaa gtcgtcgatc gcgatcgcgc cgcgctcggt
    12241 cagcgccacg ccggtgttct ccagtccgta gccggtggtg cgcggctgga agcccaggga
    12301 ctgcagcacg cgttcggcct ccagcacctt gccctctccc ccggcggcag ggctgacgtg
    12361 gacgcggacg ccctcggccc actcctcgat cgagtcgacc ttggtgctgg tgagcacggt
    12421 cacgccgagc ttcttgtacg ccttgagcag ttcggccgct acctctggat cctcgttggg
    12481 caccatccgg tcgaagtact cgacgatcgt cacctggacg ccgtagttgg ccagcacgta
    12541 ggcgaactcg gtgccgatcg cgccggcgcc gcagatcacg atcgattccg gcagctgatt
    12601 ggagaggatc tgctcctcat aggtgaagat ccgggcgctg cgggtggtgt tgggcagcag
    12661 cttggtggtt gcgccgacag cgataatgca gctgtcgaag gtgatcgtgt cctcgccggc
    12721 gtccgtggcg accttgatcg tcttggcatc cacgaaggtg ccccagccgt tgatctcggt
    12781 gatcttgttc ttccgcatca ggaagtgcac gcccttgacc atgcgatcgg agactgcccg
    12841 gctgcggtcg aacgccacgc cgtagttgaa gctcacctcg ccctcgatgc cgaaggtctt
    12901 cgcttcgcgg ttgaagatgt gcgcgagttc ggcgttgcgc agcaacgcct tcgtcgggat
    12961 gcagccgacg ttcaggcaca ccccgcccca ccactgcttc tcgatcaccg ctacgctctt
    13021 gccgagctga gctgcgcgga tggctgcgac gtagccgcca ggacccgcac cgaggatgac
    13081 gacatcgaag tgttggctca tgccggttac tctatccact gtcctgacga gcggcgcagg
    13141 gggcgcctac ggattggcgg cgagtcgcaa cccagttgct catatctaat gtgagataag
    13201 ctgtcgctcg tgaaggacct tctaccgagc agtctgggca tgctgattcg ggacgcgcgc
    13261 aagcagcgcg gcctgaccca acaggaactc gcggacgtgc tcggcacctc ccagagcgcg
    13321 gtgcaccgca tcgaatccgg cgggcagaac ctgtcgctgg aaatgatcaa tcgcatcgcc
    13381 accgccctgg acagcccgct gatcaccgcc ggcagcggcg ggccgacgca cctacgcatt
    13441 cagggcccag tgacgctgtc gggtgagatc gccgtccgct cgtccaagaa tgcagcggtg
    13501 gccctgctgt gcgctgcctt gatcaaccac gggcggacgg tgttgcacgg catcgcccag
    13561 atcgaagagg tgaaccgcat cctcgaggtg ctcaccagca tcggcgtccg ggcgacctgg
    13621 agcgaggaca agtcagagct cgtgctggtg cgtccggcca agctcgacct tgccgacatg
    13681 gacgtggccg ctgcgcgccg gacgcgctcg atcatcatgt tcctcgggcc gctgctgcat
    13741 tccgaggaca cgttcgacct gccctatgcg ggcggctgca gcttgggcgc gcgcacgatc
    13801 acgccgcacc tgcaggcact gcgacatttc gggctcagcg tccgcaccac tcagggcctg
    13861 taccacgccg atgtcgacca ggcctgcacc ggcgaccgtc ggatcacgct caccgagcgc
    13921 ggcgacacgg tcaccgagaa cgtgctgatg gcggccgcgc aattcccggg tcgcactgag
    13981 atccgcaacg cgtccagcaa ctacatggtg caggacctgt gcttcttcct tcgtgaactt
    14041 ggcgtgcagg tggatggcat cggcaccacc acgctcaccg tccatgggct ggaccgcatt
    14101 gacgccgatg tggcgtacac accttcggag gatccgatcg aagcgatgag cctgatcacg
    14161 gccgcgatcg tcaccggctc cgagctgacc gtgcagcgct gcccgatcga attcctcgac
    14221 atcgaactcg cgatcctggc cgagatgggc ctgaactaca gcctgaccga ggagtacgcg
    14281 gcgtacaacg gccagacccg gctcgtcgac gtgaccgtga agccgtccag actgcactcc
    14341 tccgcggaca agatccatcc gatgccgttc ccagggctca acatcgacaa cctgccgttc
    14401 ttcgcggtga ttgcggccgc ggcagagggt cggacgctga tccacgactg ggtgtacgag
    14461 aaccgcgcga tccacctgat cgagctgaac aaactgggcg cggacgtgca actgctcgac
    14521 ccgcaccgga tcgcggtgaa cggccccacc cgctggcgcg gccaggaact ggtctgcccg
    14581 caggcactgc gtccggcggt gtgcctgctg ctggccgcac tggcggcccg cggcaccagc
    14641 gtgctgcggg atgtctatgt gatcaaccgc ggctacgagg acctccccaa ccggctgaac
    14701 gcactgggcg cgaagatcga ggtcttccac gactgaacga aaaggggacg gtgcccttat
    14761 cgttcaactt gtccacagcc tcggtagcga tctggcttca ggagtggaaa acctggatga
    14821 aaaaggaacc ctcccctttt cgttcaggcg agctggcgca cctcgaagtg gtggctcggg
    14881 tcgaccagtt cctgctgggc tgcgaccagg gcgagttcgg cggactccaa ctcggcggta
    14941 cggcgcagca gtgagtagac cgcgtccgcg gtggcaccta cagcgtggcc gggggtctcg
    15001 ctgttcagca gatgcgccag gaacagcgcc gcggtcagat cacccgagcc ggtgaaattg
    15061 cgctcgagtc tcggggtggt cacctgccag gcgccatcag ctgacaccac cagcatgttg
    15121 atctcgtcgg ggtggtcgcc ttcgagcacg gtggacgtga ccaacaccac tttcggcccc
    15181 cgctgctgca gttcccgggc tgcgccgagc acttcggcca gggttgtggt ggtgcgtccg
    15241 gtgaggaagt cgagctcgaa gtggttcggc gtgaccacat ccgcggccgg caccacctgg
    15301 tcgcgcatga actccggaat gcccggacgc acgtagatac cgcggccgat atcgccgatg
    15361 accgggtcgc agcagtagat cgccgcgggg ttctccttcc gcaccagccg cacggcttgc
    15421 aggatctccg cacccatctc cgcggcaccc tggtagccgg aaaggatggc gtccacgcgt
    15481 ccgagcacgc cacgatcggc gatgcctgcc acgacgtccg ccacgtctcc gggggccagc
    15541 agcggcccac gccaggcgcc gtagccggtg tggttggaga aatggacggt gatcaccggc
    15601 cacacctcaa cacccaggcg catcagcgga aacgtggccg cactattacc cacgtggccg
    15661 taggcgaccg aggactggat cgagagaatc gttgtcaccg ccatattgtg cggccgatcc
    15721 gagccagtcg agcgtcacgt ccagccctgc ggccctagag tgccctcgtg aacgtcggcg
    15781 acttcccctt cctcgatcat cgcttcgtcg cgatagcgca tcggggtggc tggacgcggc
    15841 cggcggacgc cgccagggag aacaccgtct acgccttcga gagggcagtc gacctcggct
    15901 tcagtttcct ggagatcgac gttcgcacca ccgcggacgg gaagctggtg ctcttccacg
    15961 atgcccggct ggaacggctc accggcgcca gcgggcgggt gcgcgagcgc acctggcgcc
    16021 aactccgtga cctgcggatc ggcgggttcg atcccgtccc gctgcttgag gacgtgctgg
    16081 accggttccc ggcgacagtg ttcaacatcg acctgaagga caacgccggc gtgcccgcgc
    16141 tggtagcgct gctccgccgg atggacgtct ccgaccggat cgtggtggcg tccttctcca
    16201 ccctccgcat ccgtcgtttc gccagccgct cccccggcat cgcgctcgga gcgacgccgc
    16261 tcggcgtcgg ttgggacggt ttcgtgcccg gactgcgccg ctggcgggtc gatccggcac
    16321 cggtcctgca ggtgccggct cgggtgcttg gaaacctgtt gccgttggtg cggcgcgacc
    16381 tcatcgactt cctgcacagc accggacgga aagtgcacgt ctggacgatc gacgatcgcg
    16441 ccgagatgga gcgcctgatc gacctgggag tggacgggct gatctcgaac gacctggtga
    16501 cgctgaagtc ggtgctcact gaacgcggac tctgggaggc ggacgaatga ggagcacacc
    16561 cgcgcagcga tcctggtact ggtacgactg ggccaactcc gcctatatca ccacgactgc
    16621 gacggtgatc atcgggccct acctgaccag cctggccaac aacgctggct gcccgcagct
    16681 ggccgagggc gagatctgca ccaatccggt gctgctgctc ggcatcttcc ccgtgctgcc
    16741 gggcgcgctg caccctttcc tcggcacgct gagcacaatc gtctcggcaa tcctgctgtt
    16801 gttcgtcggc gccgcggccg accgctcgac gaagccgcac cgctggctcg ccaccaccgc
    16861 ctggatgggc gcgctggccg gaagcctgat gttcttcctt gagggcgaca actggcagct
    16921 gggcgcatgg ctgatgatca tcgcggcgtt ctgcttcggt gcctcgacgg tggtgtacga
    16981 ctcaatcctg gtgcggatcg ccggacctga cgagcgcgac aaggtctcca gccgaggctg
    17041 ggccttcggc tatctgggcg gtggcctgct cctgctcgcg aacttcgcgc tgatgagcct
    17101 ccatgagcaa ctgggcatca gctacgggct ggcggtgcgg ctcagcctgc tctccgccgg
    17161 tctctggtgg ggcctcttca cgatcatccc ggtctttggc ctgcgcaggc ttccccgcga
    17221 agtgtcgact ccggtggctc gccggaatcc gttcagccag ttgatggcca ctttcgcgag
    17281 catgcgcaac tatccgcaga cgatgctctt cctgctcgcg ttcctgttct tcaacgacgg
    17341 catccagacg gtgatcgtct cgtccagcct gtacgcgtcc tccgagctgg gcatggcgac
    17401 cagccaggtg atgatgactt tcctggtcac ccagttcgtg gcgttcggcg gtgcgctgct
    17461 gttcggccgg gttgccgcgc ggatcggtgc gaagcggacg gtgcttggcg ggatcgcgat
    17521 ctggttggcc gtggtgcttt ccgctttcct gctgccggcc ggcaacttcg gtgcgctgat
    17581 cggcctcggt gcagcgatcg ggctggtgat gggcggcacc caggcgctgt cacgctcgat
    17641 gtatagccag ctgatcccgc tcggacggga ggcggagtac ttcagcttct accaggcgat
    17701 ggagcgcggc acgagctggc tcggaaccct aacgttcgcg ctggtgttcc agttctcgca
    17761 ttcgtaccgg ctggctctgg tcgcgctggt ggttttcttc gtcgtcggcg gggtactgct
    17821 cacccgcgtg gacatgcgca agggaatcac ggacgccggc aacgcgttac caaaggtgat
    17881 ctagatggcc gccgtcacct gcgtcggtcg acccggctac ggtggaaggc ggaggtaacg
    17941 agatggctga tcgagcattg cgcgggttcg gactcggtgc gaagagtcac gccgacccga
    18001 gcggggtcga actcgcaccc cgccaggaga tcggtttcga gtgcccgaag ggccaccagt
    18061 tcaacgtcgt gttcgccgaa gaggcggaac ttccgaccga gtgggagtgc ccgcggtgcg
    18121 gcgccacttc gcgtcgttcg gacggggtgg agccggaggc caaggagacc aaggcgccgc
    18181 gaacccactg ggacatgctg cgcgagcgtc gctcgatcga ggagctcgaa gacctgctcg
    18241 ccgagcgcct cgaggagatc cgcggcgacg gccagtaggg cggcgccgaa gccgttgccg
    18301 gacttgtgcg gacgaccctt cgacaggctc agggatcgtc ggccggggcg accggatcgt
    18361 taggcgggcc gaccgccagg ttcgatttcg ccctcaatca cgacgctggt tcccgcgtcc
    18421 gttgccggcg acggcacatc gtccgcggtc tcgccgcgaa tcaccgtgcc tgggccatac
    18481 ttggccttga tcaccccgaa gtccactcct gacttcgccg cctgtcgagc gatcaggaag
    18541 cctagtgact tccgcaccag cggacgggtg agcggcagca ggaagaacac tccgatgaag
    18601 tcggtgaaga agcccggcag catcagcatg atgccgccga tcagcaccag ggccgcgtcc
    18661 gccaactgac cggtgggcat gcgtccggtg ttgattgcct gggtgagtgc cgtccaggcc
    18721 ttgctgcctt cacgacgcag cagccaagcc ccgaccagcg cctcggcgag aaggatccca
    18781 agggtcggca gtgctccgat ccgtgagccg acgaaggtca gcagccagac ctcggctacc
    18841 gcgaccagga tgaacccgac caccagcgcc ggcagcaccc aacggccggc gaacagattg
    18901 cggttcatgt cgtgcctccc tggccgcgct gctgccacca cttggtgacc tgaccacgca
    18961 ggcggcccac cttgcgtcct gcctcctgag cagcctggcc agccgcagca cccagcctct
    19021 cggtgccgtc cttcacctgg ccgagccggt agcgcaagcc ccactgggcg gtcagcagca
    19081 tcgcctccgc gacgatctgg ccactcatct tcgaatcgcc gtactcgcgc tcgatgaact
    19141 cgatcggaac ctcggccacc tcgaaaccgt ttcgcagagc acgccaggcc aggtcgacct
    19201 ggaagccata gccggcagcc tcgacgccat ccaggtcgat accgcgcaga gtggcggccc
    19261 gccatgcggt gaagccaccg gtggcgtcct tgaccttgag gccgagcatc agccgggtcc
    19321 acaggttgcc gccgcgggag agcagttcgc ggtgcaacgg ccaacctttg gtcgagccgc
    19381 ccttgatgta ccgggagccc ttgaccgcgt ccgcggtctc caggcgcttg agcatggcgg
    19441 gcaggtactc gggctggtgg gagccgtccg catcgtgttc caccagcacg tcatagccct
    19501 cagccagtcc ccagcggaag ccggcgacgt aggccgcgcc gagcccctct ttgcccgttc
    19561 ggtggagcac atgcaggtgg tcgtcggccg cagccagttc gtcggcgagg gcgccggtgc
    19621 cgtccggtga gttgtcgtca gctatcagga tgtgcgcgga cggaaccgct gcgcggatgc
    19681 gactggtgat cggtcgaagg ttttcggctt cgttgtaggt ggggatgatg acgagcacac
    19741 gctcaagacc tgccgggtag gccattcgct tctccaagtt gggtaacttc cacatccatt
    19801 ctgccgtacc gcagtgacag cccgagtccg acgactgcga gcagggtgat ggtgagctcg
    19861 atccaggacg ccccccaggc cgccggcgtg accccttcgc gggtcggcaa ctcgaccacg
    19921 ccggacgcgc tgacgtgatc cggttcggtg aaggcgacgc tcccgtccgg attgatcagg
    19981 ccgctcaccc cgctggtggt caccacgagc atctcgcggc gaagctcccg cgcgcgcacc
    20041 cgagtgatgg cgaactgctg ctcgatctgg ccggtgccct ggtacatggc gttgctggac
    20101 tgcacgatct gcacctgacc gccagcacgg acggtgtcgt aggcgaaatc gtcgtacacc
    20161 aggtcgtagc agaccaggat gcccagcttc agggcggtgc cgtccgcgag ggtgaccggc
    20221 agtacccccg gcgcagtgcc agggatggac tgcgcgccca cgtactgcag ttcggggatc
    20281 agcggcagca ggatcgagcg gaacggcacc cattcgccga agggaacaat gccccgcttg
    20341 atgtaagttc cctggacccc tgcctccggg tcccaccaca gcgaggcggt ccggcgctcg
    20401 tcttttccag gaccttccag aattgccccg accaggattg gcacgccagc gcgatccagc
    20461 gcggcctgaa ccagtctatt ggtttccgca tcgaggaacg ggtccatgtc ggtgccgttc
    20521 tcgggccaca caatgaacgc tggcggtggg cgggttcccg tgtccacgtc gtcggcgagg
    20581 cggtcggttt ctgacatctg gtcggcgaac cgggcggtct ccgcggcttg attgcgggtg
    20641 atcgtccgcg cctctccgag accgtagacg cccccgccgg gcgcgccgcc ctgcacgtag
    20701 ccgactgagg tggtgccggt ctgcagtccg gccggaacga gtaggccgcc gagggcgagg
    20761 acggcaatgc cggcgacccc tgacgcgacc aacgcggttc gttgcttgga cggcgacagc
    20821 acgagccagg cgagcagctg gcccgccatg gccgtcgaga agccgagcgt ggccacgccg
    20881 accaggggca gcatggcggc gagcggtgag tcgaccatgg cgtagccgag acgcatccag
    20941 ccgaagccgc tgaacgggaa cctggaatag gcgaactcga tgcccgtcca gcaggcggcg
    21001 gccagcagcg gccaccaggc ggtacggctg gccagcttga tcgctgcgcc gaggaccgcg
    21061 tagaacaggg cttccaccag gaccaatccg agcatggcct gcaggaagat cacctgcatc
    21121 cagccgacgc ccagcagcag gaagccaaca ccgaaggcga acccgagacc ggccgcgcca
    21181 cgcaaccggc gggcggcgag cacggcgagc gtcagcccgg ctacaccggg aatcaccaat
    21241 ggccaggctc cgaacggctc gaagccgagt gccgtcagta cgccggcgag gatcgccagg
    21301 gcggcccgca caccccagtg ccggttcgcc agccagtggg agaaggtcgg agcgcgagtg
    21361 gccgaagctg acccgaccgg ttggtcgagc tcatcgagac ccggctgtcc cggtcgatcc
    21421 gcaaacacat gcgcagccta gtggggcgac ggtagcggcc ccgaactggc agcatggggc
    21481 gatggctgac cgattgctgg cattcgatac cgcttcgctc tatttccgtg cgttcttcgg
    21541 gctgccgtcg acgttacggg ccgcggacgg gcgtccggtg aacgcggtgc gtgggttgct
    21601 ggacttcttg gcgcggttca tcaccgacta ctcccccacc caggtcgcct gcggctggga
    21661 caacaactgg cggccgacct ggcgggtgga cctggtgccg tcgtacaagg cgcatcgggt
    21721 ggcctacgga gtcgtcgaag aggtgccggc cgacctggtg ggccaggtgc cgctgatcct
    21781 tgaggtcctg gctgcgctgg ggttgtcggt gatcggcgcg gacgaccacg aggcggacga
    21841 cgtgctcggc accttcgcca ccgttgccgg cgtgccgacc gatgtggtca ccggtgaccg
    21901 cgacctcttc caattggtgg acgacgagcg cgaggtgcgc gtgctgtact gcggacgcgg
    21961 cgtcgccaac cacgatcggg tggacgcggc ctggctgctg gccaagtacg gcatcgacgg
    22021 cgcgggatat gtcgacttcg ctgtgctgcg cggtgatccc tccgacggcc ttcctggggt
    22081 gcccgggatc ggtgagcgga ccgcggcgcg gctggtggcc gactacggcg atctggaggg
    22141 catcgtggcg gcagcggtgt ccggccggct ctcccccacc ctgaccgcac ggctgaaggc
    22201 cgccgtcgac tatctgggtg cggcgcgcga ggtggtcgcg gttcgccgtg accttgatct
    22261 cgcccccgtc gacctgacgt tgcgcgccgc gccagcggat cccgtccgct tcgccgagct
    22321 ggctgaagag ctgaacctcg gcggttccgc cgagcgaatc ctagcggcca tgaaccttcg
    22381 aggcgccggc ccttcgtgac ggtcgcagcg cgacctcctc agggatcatt ttgctcggcc
    22441 tagagagggc tcaatccgtg tggttcgagc gcagcgcgta gaccgcggag ccggcgacca
    22501 cattggcgaa gcgattgccg ctgggcagcg gcgtccctgc tgggctgagc acgattcgcc
    22561 gttcgacggt cagtcccagg cggtcgaaca gttcttcgag ttccaccagg gtggtgtgcc
    22621 gcaggttggg cgtgtcgtac caggcatgcg gaatctcccg cgacatcggc atgcggccgc
    22681 gcagcaggcg caggcggtgc cgccagtagc cgaagttggg gacggagacg atcagccggt
    22741 cggcgatgcg acccatctcc tgaagcactt cgaacgggcg atggatcgtc tggatcgtgc
    22801 gcgacaagac caccacgtcg taggagtgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22921 nnnnnnnngg acgggatctc gcggaacgtc accggctgcc cggcgtgctc caggttgcgg
    22981 acgatccggc gggaatggtc cgtgccgaac cgccagtcac tcgcgaacga gagcaccagc
    23041 cagttcaccg gatggacgct gatcttcgcg agcgcgtccg gcgaggcgaa ggcgtcgaaa
    23101 tagtccatca ctcgggtcag gtagagatag ctcaaggcat cgaagcgttc caggaacacc
    23161 tggccctgat ggtcaaggta gctctccacc gcgaagtcga cgccgaaccc gggatgactg
    23221 cggctggggt gcggcttgcg accgaacttc tcagtgagtg cgtcctcact gaggtaggtg
    23281 atgtgggcca tcatccgggc gatcgacagc cccgtgtcgg ggttcacgcc ggcttcggcg
    23341 taccgtccgt ccgcgaagtt ggggtcggtc atgatggctc gccgggcgac ggccgagaac
    23401 gcgatgttct gcgcggtcag gcggctcgaa gcagcgatga ccagggcgtt tgcgatctcg
    23461 tccgggtggt ccaggcccca ctgcagcacc tgcatgccac ccagtgagcc gccaagcgcg
    23521 gccagcagtc gcgagatgcc gaggtgccgc atgagcgcgc gatgcacgtt gaccaggtcg
    23581 ctcatcatca gcaccgggaa gtccaggccg taggcgctgc ccgtggccgg gttggtcgag
    23641 gacggtccgg tggagccttg gcagccgccg atcaggttgg cgctgaccac gaagaagcgg
    23701 tcggtgtcca gcggcttgcc tggcccgatc aggttgtccc accagcccgg ccgttttgca
    23761 ccttcgtgga acccggccgc gtgggcgtcc ccggtgagcg cgtggcaaac gaacaccgca
    23821 ttgtcggcgg cagggctcag cgtcccgtag gtctcatagg ccacgtccac cggcccgagc
    23881 gtgccgccct gggcgagttc gaggggtaca tcggcgccga acaggcggac ggtctgggtg
    23941 ttcacgagac cgaccgagcc ggcctgcgtg cttcccgcca tctgttctcc tcgtccgccg
    24001 gtgggggcca aagcataccg tcggcccggt gtcggcaggg cgtgggacaa tcgctggcga
    24061 gacgatcgag aggaccggga atgagcgagc ttaaggacaa gatcaacgcc gacaaggtgg
    24121 ccgcgatgaa ggccaaggac gagatggcgc tgtccacttt gcggatggcg ctcgccgcga
    24181 tccagaccga ggaggtcgcg ggcaaggtgg cccgcgaact gtcggacgcc gacgtgctcg
    24241 ccgtcctgaa ccgcgaagtg gccaagcgca gggattccgc cgaggcgtac accacgggga
    24301 atcgcccgga gttggcggca aaggagctcg ccgaggtgga ggtgctgcag cgctacctgc
    24361 cggccgcgct ctcggacgag gagttggacg cgatcgtcac cgccgctctg gccgccgctg
    24421 ccgccgaact gggcgccgcg ccgacgctgc gacagatggg ccaggtgatc aaggccgtca
    24481 acgccgaggt ggccggacgc gccgagggcg cgcaggtcgc cgccaaggtg aaggccgcgc
    24541 tcgcctgatc aaactggcgc gacgaacagc cgagaccccc tgcggtgacc tcgtgtcgat
    24601 cagccggaaa ccgacttcgg cacgtaccgc cagaacgcgg gcacggccat tgtcaccagg
    24661 atggtgaaca ggatcaccag gagtccgccg ccaccggtgg cccaggcagt accgatgccg
    24721 ccggccgcgt agccatgcag cacgtcggcg atccgcgggc ccccggccac caccacgatg
    24781 aacacgccct gcaaccgccc gcgcacggca tcatcggctg ctgactgaag catggcctgg
    24841 cggaaggcgg ccgaagccat gtcggctgcg ccgccgaccg ccaggcaaac gagcgcggcc
    24901 accagcatcg gcgtccgcca ggctccggca agcccgacgg ccaaacccaa cagtgccatc
    24961 gccgcacccc agatcaggat gctgaccacg acggcacgtc cctgtcgggt gaccttggag
    25021 atccagccgg agagcacccc gcccagcacc gcgccgagcg gcatggccgc catcagcagc
    25081 gcgaactccg tcccgccttc aatcggtccg ccgaacgact ggtgcgcgat ctggggcgcc
    25141 agggcccgcg gcatgccgaa gatcattgcg atcaggtcca cgacgaacga catcagcagg
    25201 atcgggtggc ccttgaggta gctgatcccg tccaccacgg acttcagccc cggcgacccg
    25261 gtgcggttct cgaccgacag gctcggcagc aaccacacgg cccgcagcgt ggcgaacagg
    25321 aagaccgtgt cgatcaggta gagccaggcg tagcccagca ccgggatcag ggcgccaccg
    25381 atcatcggtc cggcgatcgc tccggccatc attacggtca tgttcagcga gttggcagcc
    25441 ggcagttggg cttcgggcag cagcctcggc agcaccgcgc tgcgcgccgg ctggttcacc
    25501 ccgaagaagg cctgctgcag ggcgaacagg ccgagcagaa gccacacatt gttgatcccc
    25561 agcgcggact gcaccgcgaa cagcccgctg gtgacgatca acccgacggt ggtcacctgc
    25621 aacagccggc ggcggtcgaa gtggtcggcc agcgcaccac cccaaagccc gaagacgatc
    25681 agtggaacca gtccgaacag gccggtgaga cccacgtagc ccgagtcctg agtcatcgcg
    25741 tagatctggg cggggacggc gaccacggtc agctgggcgc cgatcacggt gatgatgttg
    25801 gcctgccaca gccggcggaa atgcgggttg cgcaagggtg tggtgtctgc gaacacctct
    25861 cggacggcgg ccatggagcg agagcctaag cctgctcagt tcgcgaggtc gaactcggcc
    25921 aggctctggt agccggcacc gccgggcagc gactgcgaac ggatcaggct gaagcgctcc
    25981 accgcccagg tctgcgccgg gatggtgttg aacacctcca gccagccggt gccggagatg
    26041 ccgttggctc gtgcgatcgt caggtgtgga cgcaacctgc cgccctcaac ttcaacgcca
    26101 ctacgggtga cggcagtacg ggagcgcttg gccaactggg cgagttcggt cccgccagca
    26161 ctcacaccca accagaatgc ccgcgcgtga tcggggtcgg ggaaagcgcc ggcgccttcg
    26221 atcgtgaccg cgaacgggcg cgtccgcgcg gctacctcgg cgagcgccgc ttccaggtcg
    26281 gggacggcgg acgggtggac gtcggccatg aaggcgcagg tcaggtgcca actctccggc
    26341 aatgtccagc gcaggttcag ggcgttggtg cggcgcggtt cgaggaacgt gtccagggct
    26401 tcgcggaccg acgccgaggg caggaccgcg acgaagagcc gttcagccat cgacgcgcag
    26461 caccttcagt tcgtcgtgga agcggcgtac ctgaactgcg ccgaagatca gcactgcgcc
    26521 ggcgatcagg gcgaagatgc cgatcgtgat cgccagcacc gccacacccg tgtcggggtt
    26581 gaccaggaag tagattccga ggaaggcggt gaccagcccg gacaccagca cccacagcca
    26641 atgcccgccg ttggcgcgca gccggaacgc gagcacgatc tggaacaggc cgatcaccag
    26701 tgcccagatg gcgaagatca ccacgacgat ggcagcgacg gtctgcggat agcgcagcac
    26761 gagaaggccg atcacgatgc cggcgatgcc ctgaaacacc gagcttcccc agaccgtccg
    26821 cggggcgaag atgccaatgc ccagcgccac gatgccgtcc gcgagcgtga agacgccgaa
    26881 gagcatgaag agtgcagaga ccgtccagcc cggcacgaac agcgcgaaca ggccgagtgc
    26941 gatggcgaag atgccgcggg tgagggtgag ccaccacagc cgcttcacga gctcttcggg
    27001 cgctacggac acgggttcga tcaggctcac ggtcaactcc tggcgtggct ggtgcttctc
    27061 atctgggctt gacgctactc cgtccggccc ggctggtcat aggctgcgtc catggagcca
    27121 gatcaaaccg ccgccgacgc gtccgaaacc gaactgtcga cagcgatgct ggcggagccg
    27181 gacgcgacca acccaacgcc gagtggaaag cggcagctgc gggtgctcgc cgatctcggc
    27241 cacccgttcg cctgggggtt cctagcggct gccggcttgc tggtcgcgac ttcgctgggc
    27301 ggcgcgctga gtgcgctctc cacggtcgcg gtctcgatcg gcgtggcact gttcatcgcg
    27361 cttgcgctcg aaccgctggt gcgttggctg ggtaagcacg gcatgtcgcg cggcatgagc
    27421 atcgcggtgg tgttcgtggg tttcgcggta ttcctcgggg gcgtgctcgc gatcgtgatt
    27481 ccgatggtcg cggtgcagat cacccagttc gcgcaggcca ttcccggcta catcaccaac
    27541 ctgcagaacg ccgaatggtt ccagaacctg ctggctgcca gcggttcgag tgacctctac
    27601 gagaacctgc tgaaccaggc tcgcacctgg ctctccaacc cggcgaacct gctcacgatc
    27661 ggtggtggag cgctcgcggt gggcacgggt ctgatcaaca tgatttccgg gacgatgatc
    27721 gcgatagtcc tcgccctcta cttcctggct tcgatcaaca cgatcaagtc ggcgttcacg
    27781 agcctggcac cggcctacgc gcggccacgg ttaggcaaga tgaccgacca tgtcaccgag
    27841 tcggtcggtc agtacgtgtc cggaatggcg atcctggcgc tgtgcaactc gatcttcgct
    27901 ttcatcctgc tcagcattct cggggtaccg ttcgcgttcc tgatggcgtt cctggcgttg
    27961 ctgatcacga tgattccgat gatcggttcg gtgctgttct ggctgctcgc atccctggtg
    28021 gcgctgttgc atgcgccgct caccggactg atcttcctcg tggtctattt cgcgtacatg
    28081 caggttgagg cgtacgtgat gactccgcgc gtgatgaaca aggccgtcga tatcccaggc
    28141 tcgctggtgc tgattggcgc catggtgggc ggcacgctgc tgggtctgct cggtgcgctg
    28201 atcgcggtgc ccgtaaccgc cacgctgctg atgatcctca aggaggtctt catcccgagg
    28261 caggatgcga aggtggttcc cgactaagac gactagggac gatcccctcg ttctggtccg
    28321 gaacaggcga gaccctcatc agtgtacgtt tcctgagagt ttgctgagat tcgctgcgcg
    28381 tggccgcttt ggggctgttc tggacgaaaa gtgtcagagc cgacttgttt gcttgtttca
    28441 tggatgagcg gacgggtctg gtgccggtgg aggtagcgct ggctcggatt cgtgttgcgc
    28501 tgaactcgat cgatccggat cgttcccggt tggcgccgga agcccggctg cgggtggcta
    28561 cggccgctcg gagtctgacc gggcggctgg acgcgctgac ctcgcagttg ctggctgagg
    28621 ctgacgcctc ccacgcttca gaacgcgcga cggggacgcc gacttcgacc tggctggcga
    28681 tcgatcagaa cctgaccaaa cgtgaagcgg cgggt
//
LOCUS       DAOPRQ010000030        28526 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_1308_length_28526_cov_11.750518, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000030 DAOPRQ010000000
VERSION     DAOPRQ010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 28526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28526
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1308_length_28526_cov_11.750518"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            149..1687
                     /locus_tag="PKJ61_05620"
     CDS             149..1687
                     /locus_tag="PKJ61_05620"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016455216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HOC13334.1"
                     /translation="MKKLTEAVTKARETARLGGGEARLAKQREAGKMTARERVDALID
                     PNSFCEIGLFRKNRTVTFGMDTADMPADGVVTGSATVLGRPVHIASQDFTVMGGSAGE
                     THSIKVVEMMNAALENGTPFVFINDSGGARVQEGIDSLSGYGQVFYHNVLLSGVVPQI
                     SIIAGPCAGGAAYSPALTDFVIQTRKAHMFITGPSVIKQVTGEEVTQDALGGADTHMA
                     VSGVNHFVADDDEQAILIAKKLLSFLPQNNSEEPPFIEGDYDVENDPKMLDLVPTDGK
                     QGYDIHDVIARLVDHADFLEVQAGYAANIVVGFGRINGRSVGIVANQPNVMAGVLDIN
                     ASDKGSKFIRFCNAFNIPIVTLVDVPGYMPGVAQEYGGIIRHGAKMLYAYSAASVPKI
                     TVVLRKAYGGAYIAMACKDLGADKVFAWPTAEIAVMGAEGAASIVFRKEIAAAEDPVA
                     KQAELVEEYRSTFSTPFMAASRGLVDDIIDPGDTRRKVAVALELHASKRELRPAKKHG
                     LGPV"
     gene            1697..1954
                     /locus_tag="PKJ61_05625"
     CDS             1697..1954
                     /locus_tag="PKJ61_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13335.1"
                     /translation="MSQDEELLELVRSLTDRIEGLEIKIKQLELRTEDVPEEVMVAIA
                     AAVAAYLGHRAKRRQPRFSTSRGWTSSTRRSQHSHHPLYTR"
     gene            1964..2326
                     /locus_tag="PKJ61_05630"
     CDS             1964..2326
                     /locus_tag="PKJ61_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016668188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HOC13336.1"
                     /translation="MKLKVTVNGVAYDIDVEVEEQEAPQLGAIVIGGSSGGPAVPSTA
                     SVQASSANAVVAPLAGSVARVLVEAGQEIEAGEVLLVLEAMKMETEITAPKAGKVAQV
                     LVEKGTPVQAGQALIELD"
     gene            complement(2426..3178)
                     /locus_tag="PKJ61_05635"
     CDS             complement(2426..3178)
                     /locus_tag="PKJ61_05635"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012918667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOC13337.1"
                     /translation="MSERRLALVTGASSGIGAATARSLAAAGFEVICAARRADRIESL
                     AAEIGGRAVACDVTSADEVAALAASIGDRLAVLVNNAGGAFGQEPVAAADTDAWERMY
                     AVNVIGTERVTRALLPALVSARGAIVFVTSVAADGPYEGGAGYCGAKAAERMIAGTLR
                     LEFFDQPVRITEISPGMVHTEEFSLTRYGGDQDRADAVYEGVPNPLRAEDVAEAITWM
                     ATRPEHVNIDRLTIRPVAQPAQHKVFRGTYSA"
     gene            3352..4101
                     /locus_tag="PKJ61_05640"
     CDS             3352..4101
                     /locus_tag="PKJ61_05640"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002549579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglyceromutase"
                     /protein_id="HOC13338.1"
                     /translation="MTTKLILLRHGESEWNAKNLFTGWVDVDINEKGIGEAKRGAELL
                     KAEGLLPDVVHTSVLRRAIHTAYLALDGCDRHWIPVRRSWRLNERHYGNLQGKNKAET
                     LEAYGEEKFMLWRRSYDTPPPELDADNTYTQFDDPRYAFLPPEARPLTECLKDVVIRM
                     LPYWYDSIVPDLRDGKVVIVVAHGNSLRALVKHLDGISDADIAGLNIPTGIPLVYELD
                     DDLRPTVPGGRYLDPEAAAAAIEAVKNQGKK"
     gene            complement(4273..4938)
                     /gene="phoU"
                     /locus_tag="PKJ61_05645"
     CDS             complement(4273..4938)
                     /gene="phoU"
                     /locus_tag="PKJ61_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HOC13339.1"
                     /translation="MRDNYHESLESVVTDLVSMSESVQVAVREATVALLEADLATAER
                     VISADAQIDTLHDELEARCFTLLARQAPVAGELRTVVAAMRMVADLARMGDLAAHVAK
                     IARLRYPEHAIPEGLVANFTLMARVAEEMVQTAGRSLRERNAHDAGKLAESDEEMDEL
                     RKSQFRVMLDKEWPYTVEAAVDVALLGRYYERIADHAAIMASRVIYVVTGLFPEGENW
                     PVA"
     gene            5139..6260
                     /locus_tag="PKJ61_05650"
     CDS             5139..6260
                     /locus_tag="PKJ61_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002531186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOC13340.1"
                     /translation="MTPIALGLLGLVLGAGLGALAVWLAVRAATARSASQIVSRSELA
                     EILAPLRAAVAVLDDTDAVLAASAFAKACGLVRGSSLGPNQVAVMLAAARAEGRPQER
                     ELRLPPGPASPAVELAVKVIPLANGGLLVVGEDRSADLRFSETRRDFVANITHELKTP
                     IGAISLLAEATDAAADDPDAVHNFLGKLSRETDRLNELVSQIIALSRLQAADPLLAAH
                     PVDVTELVTAAVGRCRHLAEAGSIQLSLAPAPGLQVLGDADQLETAVTNLVQNAIAYS
                     EPGARVAVTSHGVGDQVEVRVSDNGIGISEGDQARVFERFYRVDADRSRASGGTGLGL
                     SIVKHVAAAHGGDVTLWSKLGQGSTFTLRLPSSQDGAPA"
     gene            6257..6934
                     /locus_tag="PKJ61_05655"
     CDS             6257..6934
                     /locus_tag="PKJ61_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004810606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOC13341.1"
                     /translation="MTRVLIIEDEASYRETLGFLLAKEGFEVVEAADGAAGLAAYDRQ
                     GADIVLLDLMMPGMSGTEVCRQLRTRGSVPIIMVTARDTEIDKVVGLELGADDYVTKP
                     FSHRELVARIRAVLRRGTESELIADVIEAGGVRMDTDRHETTVNGVAVQLALKEFELL
                     EVLLRNAGRVLTRGQLIDRVWGADYVGDTKTLDVHVKRLRAKLEDDPANPVLLVTVRG
                     LGYKLNA"
     gene            7115..8068
                     /locus_tag="PKJ61_05660"
     CDS             7115..8068
                     /locus_tag="PKJ61_05660"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020390351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HOC13342.1"
                     /translation="MTDITVFSGHAHREFAEEMCDILGVELSPTRLSHFSNDCMQVQL
                     RANCRQRDVYIVQPLVPPTQENLMELLMMVDAARGASAGHVTVVMPHYAYARSDKKDA
                     PRISIGGRLVADLIHTAGANRVLTMALHAQQVHGFFSMPVDHLTALPVLAKHFKARDL
                     RNTVVVSPDFGNAKEANLFARMLGVGMAAGSKRRQADDKVVIDAIVGKVGGKDVIILD
                     DEIATAGSVVELIEAVRRYHVNSISVATTHGLFTGKAIERLNAQPDIIEIVATNTVPA
                     PAGLNRLTVTSVAPLFAEAVRRINCGESVSGLFSDEDTYGE"
     gene            8192..8677
                     /locus_tag="PKJ61_05665"
     CDS             8192..8677
                     /locus_tag="PKJ61_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014136563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HOC13343.1"
                     /translation="MTFTVGETVVYPNHGAAIIEALENRTIKGEEKLYLVLRIVGQND
                     LVVRVPACNLDLVGVRDVVDADGLERVFGVLRAPHAEEPTNWSRRYKANLEKLHSGNV
                     MKVAEVVRDLWRREKERGLSAGEKRMLAKARQILVSELALAEKVAEDRAEVLLDEVLA
                     S"
     gene            8702..9394
                     /gene="ispD"
                     /locus_tag="PKJ61_05670"
     CDS             8702..9394
                     /gene="ispD"
                     /locus_tag="PKJ61_05670"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:TIGR00453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HOC13344.1"
                     /translation="MKPVVAIVVAAGSGSRLGGEVPKALREAGGRPLVSRSLDALAAG
                     GVTRAIVVIAEGLLPEFETVLTDSPIPAAVVTGGAERQDSVLAGLEAIVVDPELAAAE
                     FVLVHDAARALVPAAVVARVIDALRSGAAGCVPVIPVVDSIRRLVASGSEIVDRSDLV
                     AVQTPQGFVREIVHRGHLHVRERGLLVTDDAAVIEALGEQVVLVDGSRDAFKITEPLD
                     LVIAEALLQGRW"
     gene            9391..9867
                     /gene="ispF"
                     /locus_tag="PKJ61_05675"
     CDS             9391..9867
                     /gene="ispF"
                     /locus_tag="PKJ61_05675"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017932950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HOC13345.1"
                     /translation="MNLRVGLGTDVHAFADGVPLWVAGLEFRDEPRGLAGHSDGDVAA
                     HAVCDALLSAAGLGDLGSNFGTNHPAWAGASGVSFLTETARRVRAAGFDVVNVSVQVI
                     GNRPKLAPRRAEAEAVLSAAVGAPVSVAGTTTDGLGLTGRGEGVAAIATALLTSAG"
     gene            complement(9864..11126)
                     /locus_tag="PKJ61_05680"
     CDS             complement(9864..11126)
                     /locus_tag="PKJ61_05680"
                     /inference="COORDINATES: protein motif:HMM:NF016143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HOC13346.1"
                     /translation="MTQQWGGQGANQQWGQPTWNRPTQAWGAPPAGTQWQPQQPQYQQ
                     PQYPQGPYQYPTQGGGYGQYQAPQPGYQQPGNFSPQNQGPKRRSPFRSLLLGLLMLAG
                     IALFAMTMISFLNGGSDDESAGEPADLPTVIVPTTEPDSSTSTPPPTEPTQEPGNAVE
                     APAPDFNPSDIPQPDTYSQATKWLTANAVYAESVANPTDCAVPELNMRTASVSQLTAH
                     LNDLTACLWKVWSPPLTSAGFELPRPPVTVYTEPITTGCGSLDDVNAVYCAADQRIYY
                     AKPLYKIFPADQQQARFVVETVLAHEFGHTIQARTGLLISSMAWEQKSSKAEAKVYSR
                     RLEVQADCLSGMFSSAVATSSGLSDAELANLAKVMYNLGDDVLSGKANYDGDHGLGRS
                     RQAWLATGLANTAVGKCNTFVAPASAVR"
     gene            11365..13056
                     /locus_tag="PKJ61_05685"
     CDS             11365..13056
                     /locus_tag="PKJ61_05685"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HOC13347.1"
                     /translation="MTRTGLRRWIAGVIATSGIFWWAAPAANAAETVQSFAVNATVNA
                     DGSLQVASTLTFDGAPPATLTQALAIRVRTADSYEYRFQIANVAVTSNGQALPAQVDA
                     GADATTVSVDTAGVTGPIEIGYTVVGAAMPTANDTTTIAWALLQGLNVPVAAVDATIA
                     VPAQFTEVDCAAGHPSSPGTCVYYGGGTHDSPSPFLHDENRAAGDVVVATVRFPRSAV
                     AANAQVRQLWTAERAFSVAPWPLGLATGLLALGGLGLWAAHRKYGRDALARSAATPTP
                     VAQFEAVGPGQNEFRVIDDVRPGEVGTLVDERVDPVDVTATLLDLAVRGHLVITQLPS
                     ETAYGVTDWTFARKHSDDRLLDYEHTLLDAVAPVQGEPVQVSNLAGSVGAVIGKVQSE
                     LYEEVVSRGWFVDRPDATRNRWSLIGWIGLGAAVLAAVLLAAFTRFGLVGLVLVALAL
                     GLLFAAQQMPWRTAAGASILGGLDALRAAIQVQPTDSVVVGKAYAGLSAVLPYAVVLG
                     GKERWLDALAAADVDDTADATELDWYHGPEGWNLADLPASLANFITTVQGTLFAR"
     gene            complement(13376..14815)
                     /locus_tag="PKJ61_05690"
     CDS             complement(13376..14815)
                     /locus_tag="PKJ61_05690"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA];
                     GO_process: GO:0035999 - tetrahydrofolate interconversion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine hydroxymethyltransferase"
                     /protein_id="HOC13348.1"
                     /translation="MTDLASTASSVYRQALGVIEQVEPRIAAATRAELADQRASLKLI
                     ASENYASLPVLMTMGTWLSDKYAEGTIGHRFYAGCQNIDTVEDVAAEHARELFGAEYA
                     YVQPHSGIDANLVAYWAILAHRIESPTLERLGAKTVNDLSEADWEELRATFNQQRVMG
                     MSLDAGGHLTHGFRHNISGKMFAQHSYGTDPATGLIDYAKLLADAKEFKPLILVAGYS
                     AYPRRVNFAKMREIADAVGATLFVDMAHFAGLVAGKVFTDEENPVPYADVVATTTHKS
                     LRGPRGGLLLATKEYAPSIDRGCPMVLGGPLGHVMAAKAVALAEARQPSFQTYAQAVA
                     DNAKMLADGLLKRGVNLVTGGTDNHLVLLDVGSFGLTGRQAESALLDSGIVTNRNSVP
                     NDPNGAWYTTGIRVGTPALTSRGFGADEFDRVADLITDVLRNTSPVTTAAGTPGKAKY
                     TLADGVADRTRAAAAELLDANPLYPGLEL"
     gene            15054..16100
                     /locus_tag="PKJ61_05695"
     CDS             15054..16100
                     /locus_tag="PKJ61_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020387490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOC13349.1"
                     /translation="MGPRNSVSMRDVAVLAQVSVGTVSNVLNSPDRVADATRARVQDA
                     IAKLGWVPNESARQLRAGRSRSIGMVVMDIANPFFIDIARGAEAFLYGEQYTVQIGNS
                     DKDAERELAILERFEQQRIGGVLYAPIDDTVERVSRLRQRGIPVVLVDRASGSADYCS
                     VGVDDLAGGRIAGQHLLDTGHRRLAFVGGPSALAQVRDRRRGLELAVEQAGADVSLLA
                     VTTPELTIEAGVSSAGELAALPAAERPTAVFAASDLLAIGLLQGFVTAGLRVPDDVAI
                     IGYDDIDFAAAAAVPLSSVRQPREEIGRRSAELLLAEIDAAEGGPSHQHEAVRFTPQL
                     VVRRSTQLKRRRKG"
     gene            16172..17689
                     /locus_tag="PKJ61_05700"
     CDS             16172..17689
                     /locus_tag="PKJ61_05700"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012917937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOC13350.1"
                     /translation="MQSKDAIPVLELKGVSKSFGAVVALRNADLTLHAGSIHAVVGEN
                     GAGKSTLVKIMAGLYQRDTGTFEMDGHPVSFRTTAEAKAAGVAVIYQEPTLFPDLSVT
                     ENVFMGRQPTGRFGRIDKAAMRAEVVELMTRLGVRIDPDRITQGLSIADQQIIEIAKA
                     ISLDARVLIMDEPTAALSGVEVDRLFAVARSLRDEGRALMFISHRFDEVFALCDTVTV
                     MRDGTYIDTVPTAETNVPAMVRMMVGRDVNELFPKIDVPIGEAVLEVSGLTSPGLFAD
                     ISFNVRAGEIVALAGLVGAGRSEVARAVFGVDGYTEGHVRLNGVALPKGKPAAAMKAG
                     IGFVPEDRRQQGLVLEAAIATNITDAIRDRLVSNAVITRKAENAAAQEWARALEVKAA
                     SLDTQAGTLSGGNQQKVVLAKWLATRPKLLIIDEPTRGIDVGTKSEVHRRMSELAGEG
                     LAILMISSELPEVLGMADRVLVMHEGRLTAELSRAEATPESVMFAATHSPEEVPA"
     gene            17686..18723
                     /locus_tag="PKJ61_05705"
     CDS             17686..18723
                     /locus_tag="PKJ61_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012917938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOC13351.1"
                     /translation="MTGIQATQDRVATVSIGSRLLGLLRRRETSILAAFLIMVIATAA
                     VSPIFVFSPDGWRNLLLNPAIYMVLAIGVAFVIITRSVDLSVGSTMGLTAFGVGSLYI
                     AWPGMPAVVAFLVGTAFGGLLGLLNGGLVAVAKVPGMVITLGTMYVFRGANILWAGST
                     RINASQMGPDFLAFGNAQILGIPALTILAVVVLVIAAWYLHNIRSGRELYAIGSAPEA
                     AELYGLPVRQRMLMSFVMSGAMAGLAGVMFAARYGTIASNAGTGYELQAIGAAVIGGV
                     AITGGSGTVIGAAFGAILLATINSALPVLGIQSFWQRAVVGALILGAIILDRVLAVRQ
                     KRKLIAERMEP"
     gene            18720..19745
                     /locus_tag="PKJ61_05710"
     CDS             18720..19745
                     /locus_tag="PKJ61_05710"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOC13352.1"
                     /translation="MSAVAVQTYPDYGQPLWKRIFLTREMAIIALLALTLLVASIAVP
                     HFADRLTAYNLFRDMFATLIIALPMALVMITGDIDISVGSMVGLSCSMLGLLYASGVP
                     FELAMVIVLLIGAAGGLLNGLLVTRIGLPALAVTIGTMALFRGIAVGLLGTSTITKFP
                     QAWTDLTKYEIGQTGIPFIMVFFVLLLGAFVYLLHFTRFGRSVYAIGLNSEAAAFSGV
                     NRERTRTILFVLAGTLSAMGGIYWTLLRGTARGDVGDGLELQVIAAVVLGGVSVFGGL
                     GALHGVVAGVLLIGALSSALRLVGVTAEVIKIITGVLLIGSVVAGSLITWIQDRRTKP
                     APAATGG"
     gene            19848..20903
                     /gene="rhaS"
                     /locus_tag="PKJ61_05715"
     CDS             19848..20903
                     /gene="rhaS"
                     /locus_tag="PKJ61_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012917940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0033296 - rhamnose binding [Evidence IEA];
                     GO_process: GO:0015762 - rhamnose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnose ABC transporter substrate-binding
                     protein"
                     /protein_id="HOC13353.1"
                     /translation="MKFTKTAVALSAAALALMSACTTPAATTAPSASAPATDAAASSA
                     PAASGDLKITILPKNLGNPYFDTSTKGMEAAAKEIGATTEQVGPAEAAADAQVQYIET
                     ATQQKVSAIALSANDQTALCDSVKAAQAAGVKVVTFDSDAPTCRDLFINQATSEGIAK
                     VLVEMAVDQSGGSGEVAVLSATANATNQNAWIDAMKKSLAADHPDIKLVDVVYGDDQD
                     QKSFDETQALLQKHPKLKVIVAPTTVGIAAAARYISTSDAKGKVMVTGLGTPNDMRAF
                     VKDGTVKEFALWNPEELGYLTVHAIKALADGTIKGAEGDKFTAGKLGDYTVGANQTVL
                     LGDPYKFNAENIDNFNF"
     gene            21122..22288
                     /gene="rhaI"
                     /locus_tag="PKJ61_05720"
     CDS             21122..22288
                     /gene="rhaI"
                     /locus_tag="PKJ61_05720"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012917942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HOC13354.1"
                     /translation="MTTFDSITDLLAVQAIELPSWAFGNSGTRFKVFGTPGTPRTVQE
                     KLADAAQVHKYTGLAPTVALHIPWDKVDDYAALAAYASDLGVALGTINSNTFQADSYK
                     FGSLTHVDDSIRQQAIDHHFECIDIMSATGSRDLKIWLADGSNYPGQADLRGRQDRLA
                     ESLAKIYDRLGPEQRIVLEYKFFEPAFYHTDVPDWGTSYAQVAALGEKAMVCLDTGHH
                     APGTNIEFIVMQLLRLGKLGSFDFNSRFYADDDLIVGAADPFQLFRILYEVVRGGGYG
                     PDSPVAFMLDQCHNVEDKIPGQIRSVLNVQEMTAKAMLVDAVALQEAQDAGDVLGASG
                     VFMDAFYTDVRADLAAWRESRGLPADPMKAFAASGYLARTAADRVGGTQAGWGA"
     gene            22386..22724
                     /locus_tag="PKJ61_05725"
     CDS             22386..22724
                     /locus_tag="PKJ61_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008363930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HOC13355.1"
                     /translation="MARYCLLGHVDAAKLEEYRRRHAAVWPELLVALRDAGWRNYSLF
                     LRPDGLLIGYCEADDLDAAQERVGATEINAKWQAEMSRLFASEGAPDEAWERLEEVFN
                     LEDQLAASGH"
     assembly_gap    22750..22849
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            22994..24484
                     /locus_tag="PKJ61_05730"
     CDS             22994..24484
                     /locus_tag="PKJ61_05730"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012917945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase family protein"
                     /protein_id="HOC13356.1"
                     /translation="MTARYFAAADLGASSGRVILGKLDGGRFELSETIRFENGPVPAD
                     DGIHTDARGLFENVRKGVQAAIEASGGALESVGVDTWGVDYGRLDASGELLESPFHYR
                     DDRTVGVPELVFAELPAEQLYATAGLQVMAINTIFQLISSREDERWGEVSKILFTPDL
                     LSYWLSGRRVAEVTIASTSALLDVAGRKWSDELCAHLAERYGLPLPAVLPELVEPGTV
                     LGETTPGLFAKPLKVVAVGGHDTASAVASIPATGTDFAFISSGTWSLVGLELEQPVLT
                     EASRQADFTNELGIDGTVRYLKNVMGLWVLSESQRAWKAAGRESDLRTLLAGAAELPG
                     LACVLDMTDERLLPPGDMPGRLLAMAAETGQELADDPVAITRCILDSLALAYRRTIAK
                     SCELAGREVSVVHVVGGGCQNSLLCQLTAEATGLKVVAGPAEGTALGNLLVQARACGA
                     LSGDRTALREVSIASSELVNYEPGLLPFTAEQWDAAELRAFGVQAR"
     gene            complement(24952..26601)
                     /locus_tag="PKJ61_05735"
     CDS             complement(24952..26601)
                     /locus_tag="PKJ61_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13357.1"
                     /translation="MSTYLVPQIATRPKAEPKTVYTLANGDLRLTTNVKTWDMQQQVE
                     REFAAAVESLGWNVKRTHGVNPATGHGFIDNQRAGMEVFKNVPTEAPLVVVEAVWQYS
                     HHILHGLRDHQGPILLVANFAGDFPGLVGMLNLHGSMVKAGIKHATLWSKDFTDEWAR
                     TKLAEWIETGAIVHDTSHVRDLPALPASPEKELGEALAVQLKTDKAIIAMFDEYCMGM
                     YNALIDDEMMNPAGIYKERLSQSALVAEMKNVSDEEADAVRSWLNEAGMTFHINNDGD
                     WKENLTDEQVHQQMKMYVAAVRMADDFGADVIGIQYQQGLKDMVPASDLVEGLLNNVE
                     RPPVTSRDGSRVLYEGAPIPCANEVDQGVAIDALVTNRVWTAMGLDPATTLHDVRWGD
                     QWGEDFVWVLEISGSVPASHLGGYDKAHAYRQSYYFFPLGGATLSGVSKPGEMVWSRV
                     FIMDGALHVDLGRGTCIEMPAEEVQRRLDATNPEWPIMNAVLHGVNRDQLMARHKANH
                     LNVVYAPDAETADAALVAKAAMFDAMGYQVHLAGDVLTDLA"
     gene            complement(26917..27936)
                     /locus_tag="PKJ61_05740"
     CDS             complement(26917..27936)
                     /locus_tag="PKJ61_05740"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOC13358.1"
                     /translation="MVTIYDVAAKAGVSPATVSRVFNGIKVSPRRVEAVRHAAEELGF
                     VPNRNARRLRTSSSEIIAMLIPDIENPFFTTMTRAVEDVARAGGYSVMLCNTDEDPQR
                     EQDYLRAAVSDPVAGIIVAPSTRDAKLDLALERGVPVVCVDRRAPNYPVDSVVADNVA
                     GAAAAAQKLVDAGYRRMACISGPEGVETADNRMAGWAETYQRATGVEPASELIVRTSY
                     TLTGGDRAARQLMALPEPPDAIFAANNKLAAGAIRALAELGKLPPEVGVVSFGGLPLV
                     LLVPAGVMEAHLPVREMGITAATMLLERIQGLDAPARDVVVPMVASAEDAEDAGLTAL
                     EAAHI"
     gene            complement(27936..28496)
                     /locus_tag="PKJ61_05745"
     CDS             complement(27936..28496)
                     /locus_tag="PKJ61_05745"
                     /inference="COORDINATES: protein motif:HMM:NF014801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HOC13359.1"
                     /translation="MPRTSGWVAELIAAAVDVAAADEGLFFLPYLIGERSPWWDADAS
                     GVFAGLRMHHRRPHLTRAVLEGVGYSLALCMAPLRANAGASGIDVIGGGAASDIWLAL
                     LADIWGVPVRRRSVTTQANSLGAAVTGLVGLGLADFSLAPTLSHVEAEFSPGANRAVR
                     ERQLARFADAYRALRPWFNADAEDAR"
BASE COUNT         4466 a         9427 c         9807 g         4726 t
ORIGIN      
        1 agctcaaggc cgaagccgag gcagccagcg gtgcagccaa ggccgtccgc ggtccgatca
       61 agtacaaggt gtcgctgggc ggacgcgatc acgacgtgac cgtcgaaatg gcgaagtgag
      121 ggctgggatg agcaagaagg caccatcgat gaagaagctg accgaagcgg tcaccaaggc
      181 gcgcgagacc gcccgcctcg gtggcggtga ggctcggttg gccaagcagc gcgaggccgg
      241 caagatgacc gcgcgggagc gggtcgacgc actgatcgat ccgaacagct tctgcgagat
      301 cggcctgttc cggaagaacc ggacggtcac cttcggcatg gacaccgcgg acatgcccgc
      361 tgacggcgtg gtgaccggct cggccaccgt gctcggccgg ccggtgcaca tcgccagcca
      421 ggatttcacc gtcatgggtg gttcggctgg cgagacgcac tcgatcaagg tcgtcgagat
      481 gatgaacgcg gccttggaga acggcacccc gtttgtcttc atcaacgact cgggcggcgc
      541 ccgcgtccag gagggcatcg actcgctgtc cggctacggc caggtgtttt accacaacgt
      601 gctgctctcg ggcgtggtgc cgcagatctc gatcatcgcc ggcccctgcg ccggtggtgc
      661 ggcctactcg cccgcgctga ccgacttcgt gatccagacc cgcaaggccc acatgttcat
      721 caccgggccg agcgtgatca agcaggtcac tggcgaggaa gtgacccagg acgccctggg
      781 cggcgcggac acccacatgg ctgtctccgg cgtgaaccac ttcgtggccg atgatgacga
      841 gcaggcgatc ctgatcgcga agaagctgct cagcttcctg ccgcagaaca actccgaaga
      901 gcccccgttc atcgagggcg actacgacgt cgagaacgat ccgaagatgc tcgacctggt
      961 gcccaccgac ggcaagcagg gctacgacat ccacgatgtg atcgcgcggc tggtcgacca
     1021 cgcggacttc cttgaggtgc aggccggcta cgcggccaac atcgtggtcg ggttcgggcg
     1081 gatcaacggc cgcagcgtcg gcatcgtggc caaccagccg aacgtgatgg ccggcgtgct
     1141 ggacatcaat gcctctgata agggcagcaa gttcatccgg ttctgcaacg ccttcaacat
     1201 cccgatcgtc accctggtgg acgtgcccgg ctacatgccg ggtgtggcgc aggagtacgg
     1261 cggcatcatc cggcacggcg ccaagatgct gtacgcgtac tcggcggcgt ccgtcccgaa
     1321 gatcacggtg gtcctgcgca aggcctacgg cggtgcgtac atcgccatgg cctgcaagga
     1381 cctgggtgct gacaaggtgt tcgcctggcc gaccgcggag atcgcggtga tgggtgccga
     1441 aggtgctgcg tcgatcgtct tccgcaagga gatcgccgct gccgaggacc cggtcgcgaa
     1501 gcaggccgag ctggtggagg aataccgcag caccttctcg acgccgttca tggccgcctc
     1561 ccgcggcctg gtcgacgaca tcatcgatcc gggcgacacc cgtcgcaagg tggcggtcgc
     1621 gctggagctg catgcgtcca agcgcgagct gcgtccggcc aagaagcacg gcctcgggcc
     1681 ggtctaggag ttggcgatgt cgcaggacga agaactgctg gaactggtgc ggtcgctaac
     1741 cgaccggatc gagggcctgg agatcaagat caagcagctg gagctgcgca ctgaggacgt
     1801 gcccgaggaa gtcatggttg ccatcgctgc cgcggtggcc gcctacctcg gccaccgcgc
     1861 caagcgtcgc cagccgcgct tcagcacgtc gcgcggctgg acgtcgagca cccgtcgctc
     1921 gcagcactcc caccacccgc tgtacacccg ctaaggaaga ccaatgaaac tgaaggtaac
     1981 tgtcaacggg gtcgcttacg acatcgacgt ggaggtggag gagcaggagg ctccgcagct
     2041 cggtgcgatc gtcatcggcg gctcgtccgg cggcccggcg gtgcccagca cggcgtccgt
     2101 gcaggcgtcg tcggccaacg ccgtggtcgc gccgctggcc ggctcggtgg cccgggtgct
     2161 ggtcgaggcc ggccaggaga tcgaggccgg cgaggtgctg ctggtgctcg aggccatgaa
     2221 gatggagacc gagatcaccg cgccgaaggc cggcaaggtg gctcaggtgc tcgtggagaa
     2281 ggggacgccg gtgcaggccg gtcaggccct gatcgagctc gactgatttc aagcagaacg
     2341 gcccgccccg attcggggcg ggccgtttct gttctcggcg ccgtccgcgt aacttgaacg
     2401 gaaaaggaac cgtccccttc tttgttcagg cggagtaggt gccgcggaac accttgtgct
     2461 gagccggttg ggcgaccgga cggatggtca gccggtcgat gttgacgtgc tccggacggg
     2521 tggccatcca ggtgatcgcc tcggccacgt cctcggcgcg cagcgggttg gggacgccct
     2581 cgtagaccgc gtccgcccgg tcctggtcgc cgccgtagcg ggtcagggag aactcctcgg
     2641 tgtgcaccat gcctgggctg atctcggtga tccgcaccgg ctggtcgaag aactccagcc
     2701 gtagcgtgcc ggcgatcatg cgctcggcgg ccttcgcgcc gcagtagccc gcgccacctt
     2761 cgtagggccc gtccgcggcc accgaggtga cgaagacgat cgcgccgcgg gcggacacca
     2821 gcgcgggcag caacgcgcgg gtcacccgct cggtgccgat cacgttgacc gcgtacatcc
     2881 gctcccaggc gtcggtgtcg gcagccgcca ccggttcctg accgaaagcg ccgccagcgt
     2941 tgttgaccag cacggccaaa cggtcaccga tggacgcggc cagcgcggca acctcgtccg
     3001 ctgaagtcac gtcgcaggcc actgcgcgac cgccgatctc cgcggcaagg ctctcgatcc
     3061 ggtcggcgcg gcgggccgcg cagatcacct cgaagccggc cgcggccagg ctgcgggcgg
     3121 ttgccgcgcc gattccactg ctcgcaccgg tcaccagggc caatcgtcgt tcgctcatgg
     3181 tctgattctg acaccagctg tgtcgccaaa tcgcgactcg attgcgctgc ggcgagctca
     3241 ggagctgtat tcagccatcg aactgggaat acgcgacaaa gacctctgcg gacactgtcc
     3301 ggacgcgtcc gcgcggctgc cgcgagcgtc ccggctcgat aggctgaccg catgactacg
     3361 aagctgatcc tgctccgcca tggtgagagc gaatggaacg ccaagaacct gttcaccggc
     3421 tgggtggacg tggacatcaa cgagaagggc atcggcgagg ccaagcgtgg cgctgagctg
     3481 ctcaaggccg aaggtttgct gcccgatgtc gtgcacacct cggtgctccg gcgggcgatc
     3541 cacaccgcct atctggccct cgacggctgc gaccggcact ggattccggt gcgtcgttcc
     3601 tggcgcctga acgagcgtca ttacggcaac ctgcagggca agaacaaggc cgagacgctg
     3661 gaggcctacg gcgaagagaa gttcatgctc tggcgccgct cctacgacac tccgccaccc
     3721 gagttggacg ccgacaacac ctacacccag ttcgacgatc cgcggtacgc gttcctgccg
     3781 ccggaggccc gtccgctcac cgagtgcctc aaggacgtcg tcatccggat gctgccgtac
     3841 tggtacgact cgatcgtgcc cgacctccgt gacggcaagg tggtcatcgt ggtggcccac
     3901 ggcaactcgc tgcgcgcgct ggtcaagcac ttggacggca tcagcgacgc cgacatcgcc
     3961 gggctgaaca tccccaccgg catcccgttg gtatacgagc tggatgacga cctgcgtccg
     4021 accgttcccg gtggccgcta cctcgacccg gaggccgcag ccgctgccat cgaagcggtc
     4081 aagaaccagg gcaagaagta gttccagcgc ggcggacacc gatcttccgg gttttcgcca
     4141 gcgcagcagt gccatcgcaa gctggacagt tgcagccgac tacggccgca actgtccggc
     4201 ttgttttagt gggaccgggc aagtcggccc gtcgaatagc agatcggcgg gcggtagggg
     4261 tgcgggaccg gctcaggcga ccggccagtt ctcgccctcg gggaacagcc cggtgaccac
     4321 gtagatcacc ctggacgcca tgatcgcggc gtggtcggcg atccgctcgt agtagcggcc
     4381 cagcaacgcg acgtcgacgg ccgcctcgac ggtgtacggc cattccttgt cgagcatcac
     4441 gcggaactgg ctcttgcgca gctcgtccat ctcctcgtcg ctctcggcga gcttgccggc
     4501 atcgtgggcg ttgcgttcac gcagcgaccg gccggcggtc tgcaccatct cctcggccac
     4561 tcgggccatc agcgtgaagt tggccaccag cccctcggga atggcgtgct cggggtaacg
     4621 cagccgggcg atcttggcga cgtgcgcggc caggtcgccc atccgggcca ggtcggccac
     4681 catgcgcatc gccgcgacca ccgtccgcag ttcaccggcg accggcgcct gtcgtgccag
     4741 cagggtgaaa cagcgggcct cgagttcgtc gtgcagggtg tcgatctgcg cgtccgccga
     4801 gatcacccgc tcggcggtcg ccagatcggc ttcgagcagc gccacggtgg cttcgcggac
     4861 ggccacctgc accgactcgc tcatggacac gaggtcggtg accacactct cgagactctc
     4921 gtggtagttg tcgcgcatct ggatcgtctt tcgtcggtag ctagttgcga tcgtaggccc
     4981 agcggggtcc tgcatgctca ggctaggcac tgggactgcg cccaaaccgg cccgcgtcca
     5041 aacgccaggt gaacgctcgg tgaatcttcc cgcggacgcc accgcagcgg gccggatccg
     5101 gcgatcggat gggacggggc ttgtctagac tccagagcat gacgccgatc gcgcttggcc
     5161 tgctgggcct ggttctaggc gccgggctcg gtgcgttggc cgtctggctg gcggtgcgcg
     5221 cggccaccgc ccgatcggcc agccagatcg tgagccggtc cgaactcgcc gagatcctgg
     5281 cgccgctgcg ggccgcagtc gcggtgctgg acgacacgga cgcggtactg gcggcgtccg
     5341 ctttcgcgaa ggcgtgcggg ctggtccgcg ggtcaagcct cggcccgaac caggtggcgg
     5401 tgatgctcgc cgccgcccgt gccgaggggc gcccacagga acgcgaactg cggcttccgc
     5461 ccggaccggc gagccctgcg gtcgaactgg cggtgaaggt gatcccgctg gccaacggcg
     5521 ggttgctggt cgtaggcgag gatcgctcgg ccgacctgcg gttcagcgag acccggcgcg
     5581 acttcgtggc caacatcacc cacgagctga agacgccgat cggcgcgatc tcgctgctcg
     5641 ctgaagccac ggacgcggcc gcggacgacc cggacgccgt ccacaacttc ctgggaaagc
     5701 tcagcaggga gaccgaccgg ctcaacgaac tggtcagcca gatcatcgcc ctctcccggc
     5761 tgcaggccgc agatccgttg ctggccgcgc acccggtcga cgtcaccgag ctcgtgaccg
     5821 ccgcggtcgg ccgctgccgg cacctggctg aggcgggttc gatccagctt tcgctggcgc
     5881 cagctccggg gctgcaggtg ctgggcgacg ccgaccaact ggagacggcg gtgaccaacc
     5941 tggtgcagaa cgcgatcgcc tactccgagc cgggcgccag ggtggcggtg accagccacg
     6001 gggtcggtga ccaggtcgag gtgcgcgtct ccgacaacgg catcggcatc agcgagggcg
     6061 accaggcccg ggttttcgaa cgcttctacc gcgtggacgc cgaccgcagc cgcgccagcg
     6121 gcggcaccgg gctgggcctt tcgatcgtca agcacgtcgc cgccgcgcat ggcggcgacg
     6181 tcaccttgtg gagcaagctc ggccagggct ccaccttcac gctgcggctg cccagcagcc
     6241 aggacggagc accggcttga ccagggtgct gatcatcgag gacgaagcca gctaccgcga
     6301 gaccctcggc ttcctactcg ccaaggaggg cttcgaggtc gtcgaagctg cggacggcgc
     6361 tgccgggctc gccgcatacg accggcaggg cgcggacatc gtcctgctgg acctgatgat
     6421 gcccggcatg tcgggcaccg aggtctgccg gcaactgcgg acgcgcggga gcgtcccgat
     6481 catcatggtc accgcgcgcg ataccgagat cgacaaggtc gtcggcctgg agctcggcgc
     6541 cgacgactac gtcaccaagc cgttcagcca tcgcgagctg gtggcccgga tccgagccgt
     6601 actacgaaga ggtacggaaa gcgaattgat cgcggacgtg atcgaggccg gcggcgtccg
     6661 gatggatacc gaccggcacg agacgacagt caacggcgtc gccgtccagc tggcgctgaa
     6721 ggagttcgag ctgctggagg tgctgctgcg caacgccggc cgcgtcctca cccggggcca
     6781 gctgatcgac cgggtctggg gcgcggacta tgtcggcgac accaagaccc tcgacgtcca
     6841 tgtcaagcgg ctgcgggcca agctggagga cgacccggcg aatcccgtgc tgctggtcac
     6901 cgtccgcgga cttggctaca agctgaacgc ctgaacgcct gcggtcgtcg tttgagatcc
     6961 cggcgtgcgc cgggacgacg ttgggtgggg gcggcctcca cgtcatcccg gcgaaggccg
     7021 ggatctcaga cccgacgccc aatgagcgcg gaagcgtctc gcgagtctcg ccggggtcaa
     7081 agtcgagccc cggcggcggt tatcgttgat ctccgtgacc gatatcaccg tcttctccgg
     7141 acatgcccat cgcgagttcg cggaggagat gtgcgacatc ctcggggtgg agctgtcccc
     7201 gacccggctc tcccacttct ccaacgactg catgcaggtg cagttgcgcg ccaactgccg
     7261 ccagcgggac gtctacatcg tgcagccgct ggtgccgccc acgcaggaga acctcatgga
     7321 gctcctgatg atggtggacg cggcgcgggg tgcgtccgcc ggccacgtca cggtagtcat
     7381 gccgcactac gcctacgcgc gctcggacaa gaaggatgcc cccaggatca gcatcggcgg
     7441 acgcctggtc gccgacctga tccataccgc aggggccaat cgcgtcctga ccatggcact
     7501 gcacgcgcag caggtgcacg gcttcttctc gatgccggtc gatcacctca ccgcgctgcc
     7561 ggtgctggcc aagcacttca aggcgcgtga cctgcgcaac accgtcgtgg tctcgcccga
     7621 cttcggcaac gccaaggagg ccaacctgtt cgcccgcatg ctcggggtgg gtatggcggc
     7681 cggttccaag cgccggcagg ccgacgacaa ggtggtgatc gacgcgatcg tcggcaaggt
     7741 gggcggcaag gacgtgatca tcctggacga cgagatcgcc actgccggct cggtcgtgga
     7801 actgatcgag gccgtccgcc gctaccacgt gaactcgatc tcggtcgcca ccacccacgg
     7861 cctgttcacc ggcaaggcga tcgagcggct gaacgcacag ccggacatca tcgagatcgt
     7921 cgcgacgaac accgtcccgg ctccggctgg gctgaatcgg ctcacggtaa cgtccgtcgc
     7981 tccgctgttt gccgaagccg tgcggcgaat caactgcgga gaaagcgtct cggggctctt
     8041 ctccgacgag gacacctacg gcgaatagcc ctctcgcctg catagataag agcggttgtc
     8101 aaccccccaa tccggctctg gctaggagag acggtggctc gtcccgcaat tcgtggtatg
     8161 ctggatagtc cggaaaggtg gtcgaagcta tatgacgttc acagtcggtg aaacggtggt
     8221 ctaccccaac catggtgcgg cgataatcga agccctggag aaccggacga tcaaaggcga
     8281 ggaaaagctg tatctcgtcc tgcgcatcgt cggacagaac gacctggtgg tccgtgtccc
     8341 ggcctgcaac ctcgacctcg tgggtgtccg cgacgtcgtc gatgccgatg gcctggaacg
     8401 cgtatttggg gtcctgcgcg ccccgcacgc cgaggagccg accaactggt cgcggcggta
     8461 caaggcaaat ctggagaagc tgcactccgg caatgtgatg aaggtggccg aggtcgttcg
     8521 cgacctttgg cgccgcgaga aggaacgcgg gctgtccgcc ggcgagaagc gaatgctggc
     8581 caaggcccgg cagatcctgg tctccgagct tgcgctcgct gagaaggtcg ccgaggatcg
     8641 cgccgaggtg cttctcgacg aggtgctcgc atcctgagca accccggtgc acactgaacc
     8701 catgaaaccg gtcgtcgcca tcgtggtggc ggccggttct ggttcccgcc tcggcggaga
     8761 ggtacccaag gcgctgcgcg aagcgggcgg ccgtccgctg gtcagtcgca gcctggacgc
     8821 actggccgcc ggtggggtga cccgggcgat cgtcgtgatc gccgagggcc tgctgcccga
     8881 attcgaaacc gtgctcactg actcgccgat cccggccgcc gtggtcaccg gtggcgccga
     8941 gcggcaggat tcggtgctgg ccgggctgga ggcgatcgtc gtcgatcccg aacttgccgc
     9001 agccgagttc gtgctggtgc acgacgcggc tcgagcgctc gtgccggcag cggtggtggc
     9061 ccgtgtgatc gatgcgctca gatccggtgc ggccgggtgt gtgccggtga tccccgtggt
     9121 ggactcgatc cggcgtctgg tcgcgtccgg ctctgagatc gtcgaccgct cggacttggt
     9181 cgcagtgcag acaccgcagg gtttcgtccg cgagatcgtg catcggggcc acctgcacgt
     9241 gcgagagcgc ggcctgctgg tcaccgacga cgcggccgtg atcgaggctc tgggcgagca
     9301 ggtggtgctg gtggacggga gcagggatgc gttcaagatc accgaaccgc tcgatctggt
     9361 tatcgccgaa gcgctgctgc agggtcgctg gtgaacctgc gggtcggtct gggcaccgac
     9421 gtgcatgcgt tcgcggacgg ggtgccgctc tgggttgctg ggttggagtt ccgcgacgaa
     9481 ccgcgcgggc tggccgggca ttcggacggg gacgtggccg cgcacgcagt ctgtgacgcc
     9541 ttgttgtcgg ccgccggtct tggcgatctg ggcagcaatt tcggcacgaa tcacccggct
     9601 tgggccgggg cttccggggt gtcgttcctc accgagacgg cgcgccgggt gcgggcggcg
     9661 gggttcgacg tggtgaacgt gtccgtccag gtgatcggca atcgacccaa gctggcgccg
     9721 cgtcgtgccg aggccgaggc ggtgctctcg gccgcggtgg gcgcaccggt gagcgtcgct
     9781 ggtaccacca cggacggatt gggccttacc ggtcgaggtg agggtgtggc cgcgatcgcg
     9841 acagcgctgc tcacgtccgc gggttagcgc accgcgctcg ccggagccac gaaggtgttg
     9901 cacttgccga cggcggtgtt ggccagcccg gtggccagcc aggcctgtcg cgagcgaccc
     9961 agaccgtggt cgccgtcgta gtttgctttg ccgctgagca cgtcgtcgcc gaggttgtac
    10021 atcaccttgg ccaggttggc gagttccgcg tccgagaggc cggacgaggt cgccaccgcg
    10081 gaactgaaca tcccggagag gcagtcggcc tgcacttcga gccgccgcga gtacaccttg
    10141 gcctcggcct tggacgactt ctgctcccag gccatggacg agatcagcag tcccgtccgc
    10201 gcctggatgg tgtgcccgaa ctcgtgggcc agcacggtct cgaccacgaa gcgtgcctgc
    10261 tgctggtcgg cagggaagat cttgtacagc ggcttggcgt agtagatgcg ctggtcagcc
    10321 gcgcaataga cggcgttgac gtcgtccagg gagccgcagc cggtggtgat cggttcggtg
    10381 tagacggtca ccggggggcg cggcaactcg aaccctgcgg aggtcagcgg gggtgaccac
    10441 accttccaca ggcaggcggt gaggtcattc aggtgggcgg tgagctggct caccgaggcc
    10501 gtccgcatgt tcagctcggg cacggcgcag tcagtcgggt tggccaccga ctccgcgtag
    10561 acagcgttgg cggtgagcca cttggtggcc tgtgagtagg tgtccggctg cgggatgtcg
    10621 gacgggttga agtcgggtgc gggcgcctcc acggcgttgc ccggctcctg ggtgggttcg
    10681 gtcggcgggg gagtggacgt cgacgagtcc ggctcggtgg tcggcacgat cacggtcggc
    10741 aggtcagcgg gctcgcctgc ggactcgtcg tccgagccgc cgttgaggaa gctgatcatg
    10801 gtcatggcga agagtgcgat gccggccaac atcagcagtc cgagcaggag gctgcggaac
    10861 ggactgcggc gcttcgggcc ctggttctgc gggctgaagt tgcccggctg ctgatagccc
    10921 ggctgcggag cctggtactg gccgtagccg ccaccctggg tcgggtactg gtagggaccc
    10981 tgcggatact gcggctgctg gtattgcggt tgctgcggct gccactgcgt gccggcgggg
    11041 ggcgcgcccc aggcctgcgt cggccgattc caggtgggct ggccccattg ctggttggcg
    11101 ccctgtcctc cccactgctg cgtcaccctt ccagcgtaga cgccatggcc cgcgtccgag
    11161 gctgccggcc catcacaagg tggacactcc gcatcgtccg gacgaggaac cgttcactct
    11221 gtcggtgggc gacgcccggc gccatccgca gccgacggtt gagccgtact gcggtcacag
    11281 gagcggcccg gccactaggg tgtcagttgg ctgaaccgat ccatgatctt cagccggcgg
    11341 acctggactg gacaggagca ggaaatgact cggaccgggc tccggcgctg gatcgccggt
    11401 gtgatcgcca cttcaggcat tttctggtgg gccgctccgg cggcgaacgc tgcagagacc
    11461 gtccagtcgt tcgcggtgaa cgccacggtc aacgcggacg ggtccctgca ggtggcctcg
    11521 acgctcactt tcgacggtgc cccgcccgcc acgctcaccc aggcgctggc gatccgcgtc
    11581 cgcaccgccg acagctacga gtaccgcttc cagatcgcca atgtggcggt caccagcaat
    11641 ggccaggccc tgccggctca ggtcgatgcc ggtgcggacg cgaccacagt cagcgtggac
    11701 accgccggcg tgaccggccc gatcgagatc ggctacaccg tcgtcggcgc ggcgatgccg
    11761 accgccaatg acaccacgac catcgcctgg gccctgctgc agggcctgaa tgtgccggtg
    11821 gctgcggtcg atgccaccat cgccgtcccc gcgcagttca ccgaggtcga ctgcgcggct
    11881 gggcacccgt cctcgcccgg cacctgtgtc tactacggcg gtggtacgca cgactcgccg
    11941 agcccgttcc tgcacgacga gaaccgggcg gccggcgacg tggtcgtggc caccgtccgc
    12001 ttcccgcgca gtgcagtcgc ggccaacgcc caggttcggc agctgtggac ggccgagcgc
    12061 gccttctcgg tggcgccgtg gccgctcggg ctggcgaccg gtttgctggc tctcggcggg
    12121 ctcgggctct gggccgccca tcgcaaatac ggacgggacg cgctcgcacg cagtgccgcc
    12181 acgcccacgc cggtggcgca gttcgaggcg gtcggcccgg ggcagaacga gttccgggtc
    12241 atcgacgacg tccgtccggg cgaggtgggc acgctcgtcg acgagcgcgt cgacccggtg
    12301 gacgtgaccg cgaccctgct cgacctggcc gtccgcggac acctggtgat cacgcaactg
    12361 ccgtcggaga ccgcctacgg cgtgaccgac tggaccttcg cccgcaagca ctccgacgat
    12421 cggctgctgg actacgagca cacgctgctg gacgcggtgg ccccggttca gggcgagccg
    12481 gtgcaggtgt ccaacctggc cggcagcgtc ggcgcggtga tcggcaaggt gcagtccgaa
    12541 ctgtacgaag aggtcgtttc gcgcggctgg ttcgtggacc gaccggacgc gacccgcaac
    12601 cgctggagcc tgatcggttg gatcgggctc ggggcggctg tgctggccgc tgtgctgctg
    12661 gcggccttca cccggttcgg cctggtggga ctcgtgctgg tcgccctcgc gctcgggctg
    12721 ttgttcgcgg cccagcagat gccttggcgc accgctgcgg gagcgtccat ccttggcggt
    12781 ttggacgcct tgcgcgcggc gattcaggtg cagccgaccg attcggtggt ggtcggcaag
    12841 gcctacgccg ggttgtcggc cgtcctgccg tacgcggtgg tgctcggcgg caaggagcgc
    12901 tggctcgacg cgctggccgc agccgatgtc gacgacaccg cggacgcgac cgaactcgac
    12961 tggtaccacg gccccgaggg ctggaatctg gccgacctgc ccgcgtcgct cgcgaacttc
    13021 atcaccaccg tgcagggcac gctgttcgct cgctgaaacg gtccctgagg agccgtccgc
    13081 gattaacgaa cggacggcga cacgttggcc gccgtcatga tccggctctg gtggctcccg
    13141 gtacatcgtg aacggtgacg gccagattcg gagctgaacc cggtttgggt tgcagatggc
    13201 gatggttgga tccaacccag cgaatggacc ccgacttcga tggcatttcg ggctcccaga
    13261 tggcttgcca tcaaactcgg gtcacgaacc ggagccacgt cgcgacggtg ctgataagcc
    13321 gtccaaatcg ggttgctcag cggtccgcga tcacgctcct gagtaaccga ttgggctaca
    13381 gctccaggcc ggggtagagc gggttggcgt ccagcagctc ggcggcggcg gcccgcgtcc
    13441 gatcggcgac accgtcggcg agggtgtact tggccttgcc gggcgtaccg gcggcggtgg
    13501 tcaccgggct ggtgttccgc agcacgtcgg tgatcaggtc ggcgacccgg tcgaactcgt
    13561 ccgcgccgaa gccgcgactg gtcagcgcag gcgtgccgac gcggatgccg gtggtgtacc
    13621 aggcgccgtt gggatcgttc ggcaccgagt tgcggttggt gacgattccg gagtccaaca
    13681 gcgccgactc ggcctgccgc ccggtcagtc cgaacgagcc gacgtcgagc agcaccaggt
    13741 ggttgtcggt gccaccggtg accaggttca cgccgcgctt cagcaggccg tccgccagca
    13801 tcttggcgtt gtcggccacc gcctgcgcgt aggtctggaa ggacggctgc cgcgcctcgg
    13861 ccagcgccac cgccttggct gccatcacgt ggccgagcgg acctcccaga accatcgggc
    13921 agccgcggtc gatgctcggc gcgtactcct tggtcgccag cagcaggccg ccgcgggggc
    13981 cgcgcagcga cttgtgggtg gtggtcgcga ccacgtccgc gtagggcacc gggttctctt
    14041 cgtcggtgaa caccttgccg gcgaccaggc cggcgaagtg ggccatgtcg acgaagaggg
    14101 tggcgccgac cgcgtccgcg atctcccgca tcttggcgaa gttcacccgg cgcgggtacg
    14161 ccgagtagcc ggccaccagg atcagcggct tgaactcctt ggcgtccgcc agcagctttg
    14221 cgtaatcgat cagcccggtc gccggatcgg tgccatagga gtgctgggcg aacatcttgc
    14281 cggagatgtt gtgccggaaa ccgtgggtca ggtggccgcc cgcgtccagg ctcatgccca
    14341 tcacgcgctg ttggttgaag gtggcccgca gttcttccca gtcggcctcg gaaaggtcgt
    14401 tcacggtctt cgcgccgagc cgttccaggg tcgggctctc gatccggtgg gcgaggatcg
    14461 cccagtaggc caccaggttc gcgtcgatgc cggagtgcgg ctggacgtag gcgtattcgg
    14521 cgccgaacag ttcccgggcg tgctcggcgg caacgtcttc gacggtgtcg atgttctggc
    14581 agccggcgta gaagcggtgc ccgatggtgc cttcggcgta cttgtcgctg agccatgtgc
    14641 ccatggtcat cagcaccggt agggacgcgt agttctcgct ggcgatcagc ttcagtgacg
    14701 cgcgctggtc ggcgagctca gctcgggtgg ctgcggcgat ccgcggctcc acctgctcga
    14761 tcaccccgag ggcttggcga tagacagacg aagcggtgct ggcgagatcg gtcacggtgc
    14821 tctcctggat cggcgacccg aacactctat cgagggcacc cccgctagcc gagcccctct
    14881 cgcatcgtcg aacgcccggc tcgcgcgtgc cagcgtccgc ctggagcacg caggcttgac
    14941 cagcactact ggacgccttg aacaggctgt gtccgttgac ggtcaatctt gatacgattc
    15001 aagcggtccg ttccgtgatc gaatcgtgac tattgatacg caaaggagcg gtggtgggtc
    15061 cgaggaactc ggtgagcatg cgcgatgtcg cggtgctggc ccaggtgtcg gttggcaccg
    15121 tctcaaatgt gctgaattcg cccgaccgtg tggcggacgc tacccgggca cgagtgcagg
    15181 acgcgatcgc caagctgggg tgggtgccga acgagtccgc ccgccagttg cgcgccggcc
    15241 gcagtcgctc catcggcatg gtcgtaatgg acatcgccaa cccgttcttc atcgacatcg
    15301 cgcggggcgc ggaggcgttc ctctacggcg agcagtacac cgtccagatc gggaacagcg
    15361 acaaggatgc cgagcgtgag ctggccatcc tggagcggtt cgaacagcaa cgcatcggcg
    15421 gcgtgctcta cgcgccgatt gacgacacag tggagcgggt ttcccggctg cggcagcggg
    15481 gcattccggt ggtgctcgtc gaccgagcgt ccggctctgc ggactactgc tcggtgggcg
    15541 tggacgacct tgccggaggc cggatcgccg gccagcacct gctggacacc ggacatcgcc
    15601 ggttggcctt cgtcggcgga ccgagcgcgc tcgcccaggt gcgcgaccgg cgtcgcggcc
    15661 tggagttggc cgtcgaacag gccggcgcgg acgtctccct gctagcggtt accacgccgg
    15721 agctcaccat cgaagccggg gtgagcagcg ccggcgagtt ggccgcgctg ccggccgcgg
    15781 agcgtccgac cgcggtcttc gctgccagcg acctgctggc gatcgggttg ctgcagggct
    15841 tcgtcaccgc gggcctgcgg gtgcctgacg acgtcgcgat catcggctac gacgacatcg
    15901 acttcgccgc cgccgcagcc gtcccgttgt cctcggtgcg gcagccacgt gaagagatcg
    15961 gacgccgctc tgcggaattg ctgctcgccg agatcgacgc ggccgaaggc gggccttcgc
    16021 accagcacga ggcggtgcgg ttcacgccac aactggtggt gcggcgctct actcagttga
    16081 agcgccgccg caagggttga caaggacggc gtgcgtccga gtatcgtttg aaacgttcgt
    16141 atggaacgtt tcaaacgcca atggaggccc ggtgcagagc aaagacgcaa tcccggtact
    16201 cgaactcaag ggtgtctcca agagcttcgg ggcagtggtc gcgctacgca acgccgacct
    16261 gacgctgcac gccggttcga tccacgcggt ggtcggcgag aacggcgccg gcaagtcgac
    16321 cttggtgaag atcatggccg ggctgtacca gcgcgacacc ggcactttcg agatggacgg
    16381 ccatcccgtc agcttccgca cgaccgcgga agccaaggca gccggcgtcg cagtgatcta
    16441 ccaggagccc actcttttcc ccgacctttc ggtcaccgag aacgtcttca tgggccgcca
    16501 gcccaccgga cgcttcgggc ggatcgacaa ggccgcgatg cgcgccgaag tggtcgagct
    16561 gatgacccgg ctcggcgtcc gcatcgatcc cgaccgcatc acccagggcc tgtcgatcgc
    16621 cgaccagcag atcatcgaga tcgccaaggc catctcgctg gacgcccgcg tgctgatcat
    16681 ggacgagccc acggcggcgc tgtcgggcgt cgaggtggat cggctgttcg ccgtcgcccg
    16741 cagcctgcgc gacgagggtc gggcgctgat gttcatctcg caccgcttcg acgaggtgtt
    16801 cgcactctgc gacacggtca ccgtcatgcg cgacggcacc tacatcgaca ccgtccccac
    16861 cgccgagacc aacgttccgg ccatggtgcg gatgatggtc ggacgcgacg tcaacgagct
    16921 cttccccaag atcgacgtgc cgatcggaga ggccgtcctc gaagtcagtg ggctgacctc
    16981 gcccggcctg ttcgccgaca tcagcttcaa cgtccgcgct ggtgagatcg tcgcactggc
    17041 cggcctggtc ggcgccggac gctccgaagt ggcgcgcgca gtgttcggcg tggacggcta
    17101 caccgagggt catgtgcggc tcaatggagt ggcgctcccc aagggcaagc cggcggcggc
    17161 gatgaaggcc ggcatcggct tcgtccccga agatcgccgg cagcagggcc tcgtcctgga
    17221 agccgcaatc gccaccaaca tcaccgacgc catccgcgac cgactggtga gcaacgcggt
    17281 gatcacccgc aaggccgaaa acgccgccgc ccaggaatgg gcacgcgcgc tggaggtcaa
    17341 ggccgcctcc ctggacaccc aggccggcac gctgtccggc ggcaaccagc agaaggtcgt
    17401 gctcgccaaa tggctggcga ctcgcccaaa gctgttgatc atcgatgaac ccacccgcgg
    17461 catcgatgtc ggcaccaagt ctgaggtgca ccggcgcatg tccgaactgg ccggcgaggg
    17521 cctcgcgatc ctgatgatct cgtccgagct gcccgaggtg ctcggcatgg ccgaccgcgt
    17581 cctggtgatg cacgaaggcc gcctcaccgc cgaactcagc cgcgccgagg ccacgcccga
    17641 atcggtcatg ttcgccgcaa cccactctcc cgaggaggtc cccgcgtgac cggcatccag
    17701 gccacccagg acagagtggc aaccgtgagc atcggcagtc gtctgctcgg cctgctgcgt
    17761 cggcgggaga cgtccatcct ggcggccttc ctgatcatgg tgatcgccac cgctgccgtc
    17821 agcccgatct tcgtcttcag cccggacggg tggcgcaacc tgctgctcaa ccccgcgatc
    17881 tacatggtgc tggccatcgg cgtggcgttc gtgatcatca cccggtcggt ggacctgtcc
    17941 gtcggctcga cgatgggttt gaccgcgttc ggagtgggtt cgctctacat cgcgtggccc
    18001 ggcatgccgg cggtggtggc cttcctcgtc ggcactgcgt tcggcggcct gctcggcctg
    18061 ctgaacggcg gcctggttgc ggtggcgaag gtgcccggca tggtgatcac gctcggcacg
    18121 atgtacgtct tccgcggcgc aaacatcctg tgggccggct ccacccggat caacgcctcc
    18181 cagatggggc cggacttcct cgccttcggc aacgcccaga tcctcggcat tccagcgctg
    18241 accatcctcg cggtcgtggt gctggtgatc gccgcctggt acctgcacaa catccgctcc
    18301 ggacgcgagc tgtacgccat cggttccgcc cccgaggccg ccgaactcta cggcctgccg
    18361 gtgcggcagc ggatgctgat gtcgttcgtg atgtccggtg cgatggccgg cctggccggg
    18421 gtgatgttcg ccgcccggta cggcacgatc gcgtccaacg cgggcaccgg ctacgaactg
    18481 caggcgatcg gcgcagcggt gatcggtggc gtcgcgatca ccggtggctc cggcacggtg
    18541 atcggtgcgg ccttcggcgc gatcctgctg gccacgatca actccgcact tcccgtcctc
    18601 ggcatccagt cgttctggca gcgcgctgtc gtcggcgccc tcatcctcgg cgcgatcatc
    18661 cttgaccggg tgctcgccgt ccgccagaaa cgaaagctca tcgcggaaag gatggagccg
    18721 tgagcgccgt cgccgtccag acctaccccg actacggcca gccgctgtgg aagcggatct
    18781 tcctcacccg tgagatggcg atcatcgcgc tgctggccct caccctgctg gtcgccagca
    18841 tcgccgtccc gcatttcgcc gatcggctga ccgcctacaa cctgttccgg gacatgttcg
    18901 ccacgctgat catcgcgctg ccgatggcgc tggtgatgat caccggcgac atcgacatct
    18961 cggtgggctc gatggtcggc ctgtcctgct cgatgctggg gctgctgtac gcgtccggtg
    19021 tgccgttcga gctggcgatg gtgatcgtgc tgctcattgg cgcggccggc ggtctgctca
    19081 acggcctgct ggtcacccgg atcgggctgc ctgcgctggc ggtgacgatc ggcacgatgg
    19141 cactgttccg tggcatcgcg gtgggcctgc tgggcacctc cacgatcacg aagttcccgc
    19201 aggcctggac cgatctgacc aagtacgaga tcggccagac cggaatcccg ttcatcatgg
    19261 tgttcttcgt gctgcttctg ggcgccttcg tctatctgct gcacttcacc cggttcggac
    19321 gcagcgtgta cgcgatcggc ctgaactccg aagcggcggc cttctccggc gtcaaccgcg
    19381 agcgcacccg gaccatcctg ttcgtccttg ccggcaccct ttcggcgatg ggcggtatct
    19441 actggacgct gctgcgcggt accgcccgcg gcgacgtcgg cgacggcctg gagctgcagg
    19501 tgatcgcggc ggtcgtactg ggcggggtgt cggtgttcgg cggcctcgga gctttgcacg
    19561 gcgtcgtcgc cggtgtgctg ctgatcggcg cactgtccag cgcgttgcgc ctggtgggcg
    19621 tgaccgctga ggtcatcaag atcatcaccg gtgtgctgct gatcggttcg gtggtcgccg
    19681 gctcgctgat cacctggatc caggaccgaa gaaccaaacc cgccccggcc gcaaccggcg
    19741 ggtagtcgaa atacccgggg tgcgccccgg cccaatgggg tcactcccaa ccaactgtcc
    19801 catctgccaa agccggtctg gtgggtcaca accgaaagga actccaaatg aagttcacta
    19861 agaccgcggt ggcgctgtcc gccgctgcct tggccctgat gagcgcgtgc accacgcccg
    19921 cggccaccac ggcaccgtcc gcctcggcgc ccgccaccga cgcagccgcc agctcggctc
    19981 ccgctgcgtc cggcgacctg aagatcacga tcctgccgaa gaacctgggc aacccgtact
    20041 tcgatacctc gaccaagggc atggaggcgg cggccaagga gatcggcgcc accaccgagc
    20101 aggtcggccc ggctgaagct gccgcggacg cccaggtgca gtacatcgag accgcgaccc
    20161 agcagaaggt ttccgcgatc gcgctctcgg ccaacgacca gaccgctctg tgcgactcgg
    20221 tcaaggctgc ccaggccgct ggcgtgaagg tcgtcacctt cgactccgac gccccgacct
    20281 gccgcgacct gttcatcaac caggcgacct ctgagggcat cgccaaggtg ctcgtggaga
    20341 tggctgtcga ccagtccggt ggctccggtg aggttgcggt gctgtccgcg accgccaacg
    20401 cgaccaacca gaacgcctgg atcgacgcga tgaagaagtc gctggccgcc gaccaccccg
    20461 acatcaagct ggtcgacgtc gtctacggcg atgaccagga ccagaagtcg ttcgacgaga
    20521 cccaggccct gctgcagaag cacccgaagc tgaaggtcat cgttgctccg accaccgtcg
    20581 gcatcgcggc tgcggctcgc tacatctcca cctctgacgc caagggcaag gtcatggtca
    20641 ccggtctcgg taccccgaac gacatgcgcg ccttcgtcaa ggacggcacc gtcaaggagt
    20701 tcgcgctgtg gaaccccgag gagctcggct acctgaccgt gcacgcgatc aaggcgctgg
    20761 ctgacggcac catcaagggc gccgagggtg acaagttcac cgccggcaag ctgggcgact
    20821 acaccgtcgg tgcgaaccag accgttctgc ttggcgatcc ctacaagttc aacgccgaga
    20881 acatcgacaa cttcaacttc tgagttgtcc ttggtgggtg cccggtgctt ccgggcaccc
    20941 accggccaag gcagtaccgg gcggtctcgc actttgaccg agacctgctc gtgcatcacc
    21001 ccgggtggtc gagcctgtcg agacctgccc ctgcaacaac cgggtggtcg agcctgtcaa
    21061 gacccccgac cggtcgcgcc taaccgcgcc cgaaggccaa ttgcgagaga agaacgctga
    21121 aatgaccact ttcgattcga tcaccgacct gctggctgtg caggccattg agttgccctc
    21181 ctgggcgttt ggcaactcgg gcacccggtt caaggtgttc gggacgccgg ggacgccgcg
    21241 gacggtgcag gagaagctgg cggacgccgc gcaggtgcac aagtacaccg gtctggcgcc
    21301 gacggtggct ttgcatatcc cgtgggacaa ggtggacgac tacgcggcgc tggccgcata
    21361 cgcgtccgat ctcggggtgg cgctgggcac gatcaactcc aacacgttcc aggccgacag
    21421 ctacaagttc ggctcgctga cccatgtgga cgattcgatt cggcagcagg cgatcgacca
    21481 ccacttcgaa tgcatcgaca tcatgtcggc aaccgggtcg cgcgacctca agatctggct
    21541 ggcggacggc tcgaactacc ccggccaggc cgaccttcgc ggacgccagg atcgcctggc
    21601 ggaatcgctg gccaagatct acgaccggct cggccccgag cagcggatcg tgctggagta
    21661 caagttcttc gagccggcgt tctaccacac cgatgtgccc gattggggca cctcgtacgc
    21721 gcaggtcgcg gcgttgggcg agaaggcgat ggtttgcctg gataccggcc accacgcgcc
    21781 gggcacgaac atcgagttca tcgtgatgca gttgctgcgg ctcgggaagc tcggctcgtt
    21841 cgacttcaat tcccgcttct acgccgatga cgacctgatc gtgggtgcgg ccgatccgtt
    21901 ccagctgttc cggattctct acgaggtggt ccgcggcggt ggctatggcc cggattcgcc
    21961 ggtggccttc atgctcgacc agtgccacaa cgtcgaggac aagatccccg gccagatccg
    22021 ctcggtgctg aacgtgcagg agatgaccgc gaaggcgatg ctggtggatg cggtggcgct
    22081 gcaggaagcg caggatgccg gggacgtgtt gggtgcgtcc ggggtgttca tggacgcctt
    22141 ctacaccgac gtccgtgccg acctggccgc gtggcgggaa tcccgcggcc tgccggccga
    22201 cccgatgaag gccttcgccg cgtccggcta cctggcccgg accgccgccg accgagtcgg
    22261 tggcacccag gccggctggg gcgcctgatc cccgtccaga acacatcgca aggtggaccg
    22321 aaagtggccg agacgggcgg tttcggtcca ccttgcgcac caggtgggag ctgaaggagg
    22381 gcatcgtggc gaggtactgc ttgctggggc atgtggacgc ggcgaagctg gaggagtatc
    22441 ggaggcggca cgccgcggtg tggccggagt tgttggtcgc gctgcgggac gcgggctggc
    22501 gcaactattc gctgttcctg cgtccggacg ggctgctgat cggctactgc gaagccgacg
    22561 atctggacgc ggcccaggag cgcgttggcg ccaccgagat caacgccaaa tggcaggccg
    22621 agatgagccg gctgttcgcg tccgagggtg cgccagacga ggcctgggag cgcctggagg
    22681 aagtcttcaa cctggaagac caactcgccg cgtccgggca ctgaccccgt ccccgtcaac
    22741 ttcgctcccn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt ccctttcggg
    22861 agcgaacttt gttcgggtcg tgcccggggg tgtcttgacg cgaagaggcc gccggggtag
    22921 gctatgaaac gattcaacgc cgtcgcaagg aagcgcccat gaacctcgtc gcgagccccg
    22981 gatcggtgac ccgatgaccg cccgttactt cgccgccgcc gaccttggtg cgtccagtgg
    23041 tcgggtgatc ctgggcaagc tggacggtgg tcgcttcgag ttgagcgaga ccatccgctt
    23101 cgagaacgga ccggtgccgg cggacgacgg catccacacc gatgcccgcg gcctgttcga
    23161 gaatgtccgc aagggcgtgc aggcggcgat cgaagccagc gggggagcgc tcgagtcggt
    23221 gggcgtggac acctgggggg tcgactacgg ccgcctggac gcgtccggcg agctgctcga
    23281 aagccccttc cactaccgcg acgaccgcac ggtgggcgtg cccgaactgg tcttcgccga
    23341 gctcccggcc gagcagctgt atgccaccgc gggccttcag gtgatggcga tcaacacgat
    23401 cttccagctg atctcctccc gcgaggatga acgctggggc gaggtgagca agatcctgtt
    23461 cacccccgac ctgctcagct attggctcag tggccgccgg gtggccgaag tcaccatcgc
    23521 ctccacctct gccctgctgg acgtggccgg acgcaagtgg tcggacgagc tctgtgcgca
    23581 tctcgcggag cgctatggtc tgccgctgcc ggccgtgctg cccgaactcg tcgagcccgg
    23641 cacggtcttg ggcgaaacca ccccgggatt gtttgcgaag ccgctcaagg tggtggcggt
    23701 cgggggtcac gacactgcgt ccgcggtggc ctcgatcccg gccactggca ccgacttcgc
    23761 gttcatctcg tcgggcacct ggtcgctggt cgggctcgaa ctcgagcagc cggtgctcac
    23821 cgaggccagc cggcaggccg acttcaccaa cgaactcggc atcgacggca ccgtccgcta
    23881 cctcaagaac gtgatgggcc tgtgggtgct cagcgagagc cagcgggcgt ggaaggccgc
    23941 cggacgcgag tcggacctgc gcaccctgct ggccggcgcg gccgaactgc cggggctcgc
    24001 ctgcgtgctg gacatgaccg acgagcggct gctaccccct ggcgacatgc ccggacgact
    24061 gctagcgatg gcagccgaga ccggtcagga gttggcggac gatccggtgg cgatcacccg
    24121 ctgcatcctg gactcgctgg cgctggccta ccggcgcacg atcgcgaaga gctgtgagct
    24181 ggccggacgc gaggtctcgg tggttcatgt ggttggcggc gggtgccaga attcactgct
    24241 ctgccagctc accgccgagg cgactggcct gaaggtcgtg gccggcccgg ccgaaggcac
    24301 cgcgctgggc aacctgctgg tgcaggcccg ggcgtgcggc gcgctcagcg gcgatcggac
    24361 ggcgctgcgc gaggtgtcga tcgcttcgtc cgagctggtc aactacgaac caggcctgct
    24421 gccgttcacc gccgagcagt gggacgccgc cgagctgcgc gccttcggcg tgcaagctcg
    24481 ctgagtcgcc gggctgcgcc ggacgggtcg cagcccccag tgccggctgt tcgttacggt
    24541 tgtcgcgaat tcgaagtcca gttcgtcgcg cgcgtctgcg cggcgccttg ggccagagat
    24601 agcgctccga gttcacgaca gctgccaacc tcacgtccac ggcgccgaac gtcccacccg
    24661 gccggacggg tagcggtcag cggctggtct ccaccgggct ttgtcgcgat gtcgttgctc
    24721 atcgactcag cgggggctgg tctggggcgc tgctcgatga tccggcagcg atatcgcgac
    24781 atcggtagcg cgagcgggct atcgggtgcc gtgatttcga ccccaaattc cgcagcgcgc
    24841 ccaagcagag gcctgtgtgc ggatctcggg tcagaaagac gtcacgacct gcgacgaaga
    24901 agcgaagggc gccgccgatt agctcggtgg cgcccttcgt tctgacgaga ctcaggccag
    24961 atcggtcagc acatcgccgg cgaggtgcac ctggtagccc atcgcgtcga acatggcggc
    25021 cttggcgacc agcgccgcat cagcggtctc ggcgtcgggg gcgtacacca cgttgaggtg
    25081 attggccttg tggcgggcca tcagctggtc gcggttgaca ccgtgcagga cggcgttcat
    25141 gatcggccac tccgggttgg tggcatcgag gcgacgctgc acctcctcgg cgggcatctc
    25201 gatgcaggtg ccgcggccca ggtcgacgtg cagggcgccg tccatgatga agacccgcga
    25261 ccacaccatc tcgcccggct tggagacacc cgagagggtg gcgccgccga gcgggaagaa
    25321 gtaatagctc tgccggtagg cgtgggcctt gtcgtagccg ccgaggtggg aggccggcac
    25381 cgagccggag atctccagga cccagacgaa gtcctcgccc cactggtcgc cccagcggac
    25441 gtcgtgcagg gtggtcgccg ggtcgaggcc catcgccgtc cacacccggt tggtgaccag
    25501 cgcatcgatc gcgacgccct ggtcgacctc gttggcgcac gggatcggcg cgccctcgta
    25561 gagcacccgg gagccgtcgc gcgaggttac cggcggacgc tcgacgttgt tgagcaggcc
    25621 ctcgacgagg tcggaggcgg gcaccatgtc cttgagtccc tgctggtact ggatgccgat
    25681 cacgtcggcg ccgaagtcgt cggccatccg gacggcggcg acgtacatct tcatctgctg
    25741 gtgaacctgc tcgtcggtca ggttctcctt ccagtcaccg tcgttgttga tgtggaaggt
    25801 catgccggcc tcgttcagcc agctgcggac ggcgtccgcc tcttcgtcgg agacgttttt
    25861 catctcggcg acgagcgcgc tctgggagag ccgctccttg tagatgccgg ccgggttcat
    25921 catctcgtcg tcgatcaggg cgttgtacat gcccatgcag tattcgtcga acatggcgat
    25981 gatcgccttg tcggtcttga gctggacggc cagcgcctca ccgagttcct tttccgggga
    26041 cgcgggtagc gcgggcaggt cgcggacgtg cgaggtgtcg tggacgatcg cgccggtctc
    26101 gatccactcg gcgagcttgg tgcgggccca ctcgtcggtg aagtccttgc tccacagggt
    26161 ggcgtgcttg atgccggcct tgaccatcga gccgtgcagg ttcagcatgc cgaccaggcc
    26221 ggggaagtcg ccggcaaagt tggccaccag caggatcggg ccctgatgat cgcgcaagcc
    26281 gtgcaggatg tggtggctgt actgccagac ggcctcgacc acgaccagcg gcgcctcggt
    26341 tgggacgttc ttgaacacct ccatgcccgc gcgctggttg tcgatgaagc cgtggccggt
    26401 ggccgggttc acgccatggg tgcgcttgac gttccagccg agcgactcca ccgcagcggc
    26461 gaactcacgc tcgacctgct gctgcatgtc ccaggtcttg acgttggtgg tgagccgcag
    26521 atcaccgttg gcgagggtgt agacggtctt cggctcagcc ttgggccggg tggcgatttg
    26581 aggcacgaga taggtgctca cgagttgact ccttagggtt cggctgctca tcgccgaagc
    26641 tcccgcgaac cacgctgaac tgaccgggac gagctgcttc catgctctca gcaatcgatt
    26701 tcacagtcaa gcgtttggtg gccttcggcg gcgattctga agcgattcaa tgccgcctga
    26761 tcggacgggc gtcgacactt cacccgagcc ggtcgcggaa gggggcgtct cggcgtccag
    26821 cttgggtcgg taggcccact acagaccccg gatgtggcct tgcagctcaa cccgcccagc
    26881 acgtcctcgc tcggaacagc attgttcagc aaactcctaa atgtgcgcgg cttcgagcgc
    26941 ggtcaggccg gcgtcctcgg cgtcctctgc gctggccacc atcggcacga cgacgtcgcg
    27001 ggcgggggcg tccaggccct ggatccgctc cagcagcatg gtcgccgcgg tgatgcccat
    27061 ttcgcggacg ggcaggtgcg cctccatcac gccagcgggc accagcagca ccagtggcag
    27121 cccgccgaac gacaccaccc cgacttctgg cggtagtttg ccgagctcgg cgagcgcccg
    27181 aatcgccccg gctgccagct tgttgttggc tgcgaagatc gcgtccgggg gttcgggcag
    27241 cgccatcagt tgccgggctg cccggtcgcc accagtgagg gtgtagctgg tgcgcacgat
    27301 caactcggac gcgggctcca ctccggtcgc acgttggtag gtctcggccc agccggccat
    27361 ccggttgtct gcggtctcca cgccctctgg gccgctgatg catgccatcc ggcggtagcc
    27421 ggcgtccacc agcttctggg cggctgcggc ggcgccggcg acgttgtccg ccaccaccga
    27481 gtcgaccggg tagttcggcg cgcggcggtc gacgcagacc accgggacgc cgcgttccag
    27541 ggccaggtcg agcttggcgt cgcgggtgct gggggccacg atgatgccgg ccaccggatc
    27601 gctgaccgcc gcgcgcaggt agtcctgctc gcgctgggga tcctcgtcgg tgttgcagag
    27661 catgaccgag tagccgccgg cgcgggccac gtcttcgacg gctcgggtca tggtggtgaa
    27721 gaaagggttc tcgatgtcgg gaatcagcat cgcgatgatt tcagacgagc tggtgcgtag
    27781 ccgccgggcg ttgcggttcg gcacgaagcc gagctcctcg gcggcatgcc ggacggcctc
    27841 gactcggcgc ggggagacct tgatgccgtt gaacacccgg gagaccgtgg cgggggatac
    27901 cccggctttg gcggcgacat cgtagatcgt gaccatcagc gtgcgtcctc tgcatcggcg
    27961 ttgaaccatg gacgcagtgc gcgataggcg tccgcgaagc gtgcgagttg ccgttccctg
    28021 accgcgcggt tcgcgcccgg gctgaactcg gcttccacgt gcgaaagggt aggggcgagc
    28081 gagaagtccg ccaagcccag accgaccagg ccggtcaccg cggcaccgag cgagttcgcc
    28141 tgggtggtca cgctgcggcg ccgcaccggc accccccaga tgtccgccag caaagccagc
    28201 cagatgtcgc tggccgcacc gccgccgatc acgtcgatgc cgctcgcgcc cgcgttcgcg
    28261 cgcaacggcg ccatgcacag cgccaggctg tagccgacgc cctcgagcac ggcgcgggtg
    28321 aggtgcggac ggcggtggtg catcctgagc ccggcgaaca ccccggacgc atccgcgtcc
    28381 caccaggggg aacgctcacc gatcaggtac ggcaggaaga acaggccctc gtcggcggcg
    28441 gccacatcga ccgcggctgc gatcagttcg gcgacccaac cagatgttct cggcaacgct
    28501 caagtgcgag accaggttga gttcct
//
LOCUS       DAOPRQ010000031        28225 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_1340_length_28225_cov_12.596166, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000031 DAOPRQ010000000
VERSION     DAOPRQ010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 28225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28225
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1340_length_28225_cov_12.596166"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..492)
                     /gene="purE"
                     /locus_tag="PKJ61_05750"
     CDS             complement(1..492)
                     /gene="purE"
                     /locus_tag="PKJ61_05750"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013160647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HOC13360.1"
                     /translation="MSEPLVGIVMGSDSDWPVMSAAAEACTEFNVPYEADVVSAHRMP
                     EDMVRYGREAHARGLKVIIAGAGGAAHLPGMLAALTPLPVIGVPVPLKYLDGMDSLLS
                     IVQMPAGVPVATVAIGNARNAGLLAVRILASADPVLAEQMVAFQASLREAALAKGEAV
                     RGR"
     gene            complement(485..1702)
                     /locus_tag="PKJ61_05755"
     CDS             complement(485..1702)
                     /locus_tag="PKJ61_05755"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016669506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HOC13361.1"
                     /translation="MRPSWQNDPTGGTVPASFRLDRVTSPATTIGIIGGGQLARMMHA
                     ASIALGLRIRLLAEGPDVSAAQVVREVVVGDYTDPETVLAFAEGCDVITFDHEHVPPA
                     ILHALMDRGIAVRPGPDALLHAQDKVVMRERLSALGVPCPAFEVVPDASALVEFAERH
                     GWPVIAKTSRGGYDGKGVFKLDGPGDAESPFAGLREGVQIIAEEFIDFTRELSAIVAR
                     RPGGEMVGYTITETVQTDGICTETTTPAWFEDGADALGLIEIAERIATELDVVGILAV
                     ELMQARDGRIVVNELAMRPHNTGHWTIDGAATSQFENHLRAVADLPLGSPEQDSRWTV
                     MVNILGGTRTDLLAGLGHVLARDEYLRPQLYGKDVVPGRKVGHVTVLDDDLAAARRRA
                     HAAADYLRGLIDE"
     gene            1701..2417
                     /locus_tag="PKJ61_05760"
     CDS             1701..2417
                     /locus_tag="PKJ61_05760"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002517608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase E"
                     /protein_id="HOC13362.1"
                     /translation="MTAHIVAMGGGGFSMSPLGAPTNLDRYLVELTGKSSPLVCFAPT
                     ASADDPQYINRFLLAYGTLGVRTMILTLWEGGSAGSVARLANADLILVGGGHTVNLLA
                     LWQAHGVDKAIKELHAAGDVVLAGVSAGGSCWYEGCITDSFGDFRPWRGGLGLVPGSF
                     CPHFDGEAERAPTFTDAIAGGDLPGGYGADDGAGIHYVDGVATYFISEARGKRVYRVL
                     PSDLPTASGVLVEPQQMTIL"
     gene            complement(2452..2886)
                     /locus_tag="PKJ61_05765"
     CDS             complement(2452..2886)
                     /locus_tag="PKJ61_05765"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HOC13363.1"
                     /translation="MTKAEPGTEPAAERLALRTQLVRFVISGGLSAVVDFGLLVVLMN
                     LAGLDHTPAKALSFIAGTTTAYLINRRWTFQAEPSKRRFAAVVVLYGLTFAVQVGLFA
                     WLFPLVLSWTASQTAAQVIGFVIAQGVATTVNFIVQRGLIFR"
     gene            complement(2894..3409)
                     /locus_tag="PKJ61_05770"
     CDS             complement(2894..3409)
                     /locus_tag="PKJ61_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020576847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HOC13364.1"
                     /translation="MGLPAEQLGRDEVQVLHLRTHVKALLVPAVVLVVTAVVSGFLFA
                     LLPADWQPAAGWIVALAMLVVLAIWVVAPFLSWFTTTYTFTNRRIITRSGILVKKGHD
                     LPLARINNVSYRRGPIDQLLGCGTLVMTTAAEQPVTLNDIPDVERVHVVMTELLFGGE
                     DPIRRPTLKDE"
     gene            complement(3503..4336)
                     /locus_tag="PKJ61_05775"
     CDS             complement(3503..4336)
                     /locus_tag="PKJ61_05775"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOC13365.1"
                     /translation="MRPADGKGGNPTIDANWLARRIVSADGAWGSVATVATIGSTNAE
                     LAAAARAGAATGAVLVSDHQSAGRGRFTRVWEAPPGTSLAISALLRPPASVPLARWLW
                     LPLVAGLAVADGLRAACGVAAELKWPNDVLIGGRKVCGILTERVESPKNRTVGHGETL
                     PAVIIGMGINTTLREDELPVPTATSLALAGAEVETGEVAASVLLALDAWYRRWLAGAD
                     LRADYAERCTSVGREVRVQLSEHEAVTGRATGVDPSGCLLVESDGRERAFAAGDVFHL
                     R"
     gene            4360..5934
                     /locus_tag="PKJ61_05780"
     CDS             4360..5934
                     /locus_tag="PKJ61_05780"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002514707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HOC13366.1"
                     /translation="MELEIHTTRGKIAQLGERIEQAKNPSPQASVEKVHAQGKMTARE
                     RVLQLLDEGSFTEFDEFSRHRSTLFGLQEKRPYTDGVITGVGSIHGRPVCVFSQDVTI
                     FGGALGEVYGEKIVKIIDFAIKTGCPIIGINEGGGARIQEGVVSLDLYSQIFRRNTLA
                     SGVIPQISLIMGAAAGGHVYSPALTDFVIMVDQTSQMFITGPAVIKTVTGEDVSMEDL
                     GGGRTHSTKSGNSHYLAADENDALEYVRQLIAFLPQNNLEDPPVFEEEEADLTITEHD
                     LKLDTIVPDSPNQPYDMHEVIRGVLDDDEFLEVHALYAQNIICGFGRVEGRSIGVIAN
                     QPMVFAGCLDIKASEKAARFVRTCDAFNIPILTFVDVPGFLPGTDQEWDGIIRRGAKL
                     IYAYAEATVPLLTVITRKAYGGAYCVMGSKPLGADVNLAWPTAQIAVMGAEGAVNILY
                     KRELAAADDPTSRREELIDEYNDELANPYVAAERGYVDKVIYPHETRAQVIRVLRLLR
                     TKRQQLPPKKHGNIPL"
     gene            5931..6155
                     /locus_tag="PKJ61_05785"
     CDS             5931..6155
                     /locus_tag="PKJ61_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015071092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit epsilon"
                     /protein_id="HOC13367.1"
                     /translation="MSTEPEQLVQIVAGQPTDEELAALGAVIVALRRARPEPHAPVTN
                     TLAGGWRSYWHTVRNPLLPGREAWRSTFRS"
     gene            6197..6961
                     /locus_tag="PKJ61_05790"
     CDS             6197..6961
                     /locus_tag="PKJ61_05790"
                     /inference="COORDINATES: protein motif:HMM:NF017853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HOC13368.1"
                     /translation="MSVIDEILGSIDPEQLAAAVGTDQQTAMDAAAAAIPTLINSLQA
                     NATASADGEASLLQALGQHADGIFGDQVDLGNVDTEDGRKIVAHALADDPQRLQALGG
                     FGGGLLSKLLPLLAPIVMSYLSKKLLGGGASSGGSGNVLGDLLGGILGGGATQQSAGG
                     GLGDLLGSILGGGSAQQSSGGGLGDLLGSVFGGQEPAAVPAGVGVDTSGQVFNAPDGS
                     MQINTDAPVQTYEQPPQQQQQGGDILGDLLGQILGR"
     gene            6975..7568
                     /locus_tag="PKJ61_05795"
     CDS             6975..7568
                     /locus_tag="PKJ61_05795"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017934697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HOC13369.1"
                     /translation="MVLASASPARLETLTRAGLRPQVIVSAVDEDAFTATKVTELVAV
                     LAQAKAEAVAAVLPEDGDVLVVACDSLLDLDGRPLGKPATPEEAIQRWQEMRGRTGHL
                     HTGHHVIGRLDGQTRSVAAVATTEVRFADVTDAEIGAYVATGEPQQVAGAFTIDGLGG
                     AFVTGITGDHHNVVGISLPLLRRLFAELGVGWHTLWA"
     gene            7834..8739
                     /locus_tag="PKJ61_05800"
     CDS             7834..8739
                     /locus_tag="PKJ61_05800"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HOC13370.1"
                     /translation="MSNANLPEVAPKAALLPAWLRAMRPKQWVKNVLVLAAPVAAGRL
                     FETPVLIATLWAFVGFSLVSASIYLINDSRDIEADRLHPTKRFRPIAAGELSSNLAYV
                     LAAITAAAAFAIGFWVAPLLGATLAFYWVLQVLYSLFLKNQPIIDLAMVAAGFLLRAV
                     AGGVASGIELSQWFLLVASFGSLFMVAGKRYSELSELGHKAGTRASLERYTKSYLREV
                     WSVSIAVVIMSYSLWAFEQGGGSAWGVPWAAISIAPFTLAVLRYAMKIDQGKAGEPEE
                     VVLGDRMLQGLAVAWVIPLAIAVFA"
     gene            8736..9422
                     /locus_tag="PKJ61_05805"
     CDS             8736..9422
                     /locus_tag="PKJ61_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOC13371.1"
                     /translation="MSIPVNRPVALVTGASKGIGRQIALALSATHHVLVGGRDATRVA
                     AVVAELESAEPFLAELTDAAAVTAAVAGIDKLDVLVHSAGLVAYGPLAESTTDQWRAL
                     LELNVIAPANLTRLLLPALRAATGLVVFINSGAGLTAHPGFSAYAASKFALTALADSL
                     RGEERGRVRVTSLHPGRVATDMQAELAALEGRVYHPEVHLDPAAVARAVRLAVELPDA
                     AVDAVRINPA"
     gene            complement(9419..10045)
                     /locus_tag="PKJ61_05810"
     CDS             complement(9419..10045)
                     /locus_tag="PKJ61_05810"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HOC13372.1"
                     /translation="MTLTQAVLGFTLLAAVVTVTPGLDTVLVLRQALRGRRAVAFAAG
                     LGICVGCLVWGVAAATGLAALFVASQTAYLVLKWAGIAFLLYLAYSYLRAAIRGDSHF
                     DTSGGGTDTVREAFMKGLLTNLLNPKIAVFYVTVLPLFLPAGYPPAAIGALLAGIHAV
                     LGMVWFTVVILGANALRGFLTAGRGPRIIDAAAGTAMLGFSVVLGLEH"
     gene            complement(10166..11158)
                     /locus_tag="PKJ61_05815"
     CDS             complement(10166..11158)
                     /locus_tag="PKJ61_05815"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HOC13373.1"
                     /translation="MTSSHSRAWRDGVEIAADFPLTELDQWLAAPGVLVWFDLLQPDR
                     TLLDTLAAELSLDPLAVEDAVEPRERPKASMHATHVFLTCYATGFARLDGASVELALG
                     RVSAFVLPNALITVRPDDRVDMDDALARWTDDGLLGNGVAALVHGLLDQLVDGHFDTI
                     ETMDDMLESLEDLLFDPAMKTRVVQQRVFGLRKALVQLRRVVLPMREVVTTVRRFHTE
                     GDRATSPELAGHFNDLYDHVIRAAEWTESLRDMVTSVFETNLSLQDARLNEVMKKLAA
                     WAAIIAVPTAVTGWFGQNLPYPGYMAEWGLWQSAALILVGSIGLYILFRKVDWI"
     gene            11350..13113
                     /locus_tag="PKJ61_05820"
     CDS             11350..13113
                     /locus_tag="PKJ61_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HOC13374.1"
                     /translation="MPITKVLVANRGEIAVRVVRAARDAGLGSVAIYADPDADSLFVK
                     LADEAFALNGQTPADTYLDVAKILEIAARSGADAVHPGYGFLAENANFAQAVIDAGLT
                     WIGPPPAAIDALGDKVKARHIAQKVGAPLVHGTPDPVAGADEVVAFAKEYGLPIAIKA
                     AYGGGGRGLKVARDLGEVAELFESATREAVTAFGRGECFVEQYLDRPRHVETQCLADA
                     HGNVVVVSTRDCSLQRRHQKLVEEAPAPFLNDEQIEILYTASKAVLKEAGYIGAGTCE
                     FLVAQDGRISFLEVNTRLQVEHPVSEEVSGIDLVREMFRIADGEELGYADPSLRGHSI
                     EFRINAEDAGRNFMPAPGTVTAWVPPTGPGVRVDSGYLAGMAVPGSFDSLVAKIIVTG
                     ADRPQALARARRALAETTLEGMPSVIPFHKVVLDDPAYVAADGHFGVHTRWIETEFVN
                     EIAPYVGEMGEAEETEKNTYVVEVNGRRLEVRLPSSLAAPVVKAATAKRPVKRSTGGA
                     KAKAPSSNELSAPMQGTIVKVAVMNGDEVNEGDLVVVLEAMKMEQPITAHRAGIVANL
                     SAAVGDSVASGQALLEIVDPT"
     gene            complement(13262..14662)
                     /gene="xylB"
                     /locus_tag="PKJ61_05825"
     CDS             complement(13262..14662)
                     /gene="xylB"
                     /locus_tag="PKJ61_05825"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HOC13375.1"
                     /translation="MARVIGVDSSTQSCKVVVRDSESGALLASGRASHPAGSEVDPAH
                     WWTALQAALAQAGGLDDIAAVSIAGQQHGMVVLDADGRVIRPALLWNDTRSAGAARDL
                     IAEVGADEFVRRTGSVPVASFTITKLRWLRDAEPENAARAAAVALPHDWLTWRLRGYG
                     PASESDLGPVLEELVTDRSDASGTGYWSPSSDAYDRDLLVRALGHEALLPRVLAPLES
                     VPGPGFLVGPGAGDNAGAALGLGARAGDVVVSLGTSGTVFAAAEVPAADASGAVAGFA
                     DATGGFLPLVCTLNAARVLDVVAGLLGVDHDELARLALDAGPGAGGAVLVPYFEGERT
                     PNLPDSTATLSGLTLANTTRANLARAAVEGMLGGLGAGLDAVRAQGARIDRVLLIGGA
                     AASPAVQAVAAQVFDAPVEMPSPGEYVADGAARQAAGILGGAFPDWPVELIGAPAPDP
                     RPEISEQYAAAASRYL"
     gene            complement(14666..15856)
                     /gene="xylA"
                     /locus_tag="PKJ61_05830"
     CDS             complement(14666..15856)
                     /gene="xylA"
                     /locus_tag="PKJ61_05830"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008356885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HOC13376.1"
                     /translation="MTTTPAPSDKFTFGLWTVGYNGTDPFGGPTRHPLDVVHVVEKLA
                     EYGAYGLTFHDDDLFAFGSTDAERRNQIDRLKAACAATGLKVPMITTNLFSAPVFKDG
                     GFTSNDREVRRFALRKVLRNLDLANEVGAKTFVMWGGREGAEYDQAKDIRSALERYRE
                     AVNLLAQYVLDQGYDLKFAIEPKPNEPRGDILLPTLGHALAFIETLEHPELVGLNPEV
                     GHEQMAGLNFQAGIAQALYHGKLFHIDLNGQRGIKYDQDLVFGHGDLHNAFALVDLLE
                     NGGPNGGPAYEGMRHFDYKPSRTEDETGVWDSVQANMRTYLLLKERAAAFRADPEVQE
                     ALAAAKVTELAVPTLGDGEGYAELLADTSAYEEFDSAAYLGGKGFGFVRLQQLATEHL
                     LGAR"
     gene            16075..17238
                     /locus_tag="PKJ61_05835"
     CDS             16075..17238
                     /locus_tag="PKJ61_05835"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HOC13377.1"
                     /translation="MPAVTTEGVRRSNLAATLGILYHHGPASRAALSKRTGRNRSTIA
                     SLVADLVELGLVAESDPDPIGQVGRPSPVVSIRPEVTAIAVNPEIDALTIGLVGLDGN
                     VRRVIRHEYDRIPTASELISITAAVLAGMQLELDGLLVVGIGVALPGQVRTHDGLVRN
                     APHLGWHDEPFAEPLAAATGYRVRVANDAALGALAEHSFGAGRGKSNLIYLNGGASGI
                     GGGVIAGGRLLGGSAGYAGELGHIRVSSSSVRDSAGIAGTLEAEVTRDELQTVLGLAR
                     VTPGEFSEALLADRSDAVATVVRRQLGHLGTAIAAAINVLNPQVVALGGFLATLAEYD
                     RVLLERTVKDATLTPSMEGVSITAAELGENLLMVGAAGLAFGELLADPASIAR"
     gene            complement(17278..18690)
                     /locus_tag="PKJ61_05840"
     CDS             complement(17278..18690)
                     /locus_tag="PKJ61_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone dehydrogenase"
                     /protein_id="HOC13378.1"
                     /translation="MTNVVIIGGGPGGYEAALVGRQLGGNVTLVERRGLGGSAVLTDV
                     VPSKTLIAAAEVMSKIRSAEELGLRLLDDTRHLDTAVGIDLGAVHARIRGLARAQSDD
                     IAERLRAEGVRIINGTGRLDGPQRVVAESADGVETLDADIVLLATGAHPRELDTARPD
                     GKRIFTWTQIYDLDELPDHLIVVGSGVTGAEFASAYQALGVKVTLVSSRDKVLPGQDL
                     DAATVIQDVFEGSGMTVLNQVRAVGAEVRGERVVVTLADGAEVVGSHCLMAVGAIPNT
                     ASIGLAEAGVTVNSGGYITVDRVSRTSARGVYAAGDCTGVFALASVAAMQGRIAMWHA
                     LGDSVAPLDLRTVSSNVFTAPEIATVGVSQADIEAGKLNALIMTLPLRSNARAKMQAL
                     RDGFVKVFCLPTTRIVVGGVVVAPSASELIHVLSVAVRHKLTVDQLAEAFTVYPSLSG
                     SLAEAARRLHGHSSEQVIVY"
     gene            18750..19181
                     /locus_tag="PKJ61_05845"
     CDS             18750..19181
                     /locus_tag="PKJ61_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13379.1"
                     /translation="MPRILLAAVALAGVVVSVAMVVIGLPEANAVQSVLLELSALPLS
                     VSVAAGIGILHLGAERPPGPRGRAALLVGAGLALLGLALIVWAYLVGPRAAVHPGQVL
                     IWLGLFAALLVTVRRQPKRRLVTYEIEEDADGTSTEEDASL"
     gene            19190..19993
                     /locus_tag="PKJ61_05850"
     CDS             19190..19993
                     /locus_tag="PKJ61_05850"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002549953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HOC13380.1"
                     /translation="MTETDPKSLARSAASALLERFGIEAIDLALVLGSGWSGAAVGLG
                     EAIGECELADLPGFAKPVVAGHGGALKLIRTPAGKTAALFTGRTHFYEGCGVEPVVHG
                     VRTAAAAGAKMILLTNGCGGLNPDWAPGTPVLIRDHINLTNASPLAGATFIDLTEAYS
                     LRLRELAHEVDPSLAEGVYIQFRGPQYETPAEVRMAKVLGGDLVGMSTTLETIAAREA
                     GLEVLGISLVTNAAAGISPTPLEHAEVIAAGKAAEPRLRGLLAGLLERL"
     gene            20002..21651
                     /locus_tag="PKJ61_05855"
     CDS             20002..21651
                     /locus_tag="PKJ61_05855"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007078475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HOC13381.1"
                     /translation="MDATLRDQLAAWREQDPDVATAAALDDLLARADAGDTAAAAELA
                     DAFAGPLEFGTAGLRGPLGPGPARMNRVVVSQAAAGLAAYLNARGLTGGRVVVGYDAR
                     YNSEVFARDTAEILAGAGLLPLLCSRPLPTPVVAFGIGHFDCVAGVVVTASHNPPQDN
                     GYKVYLGDGSQIIPPTDAEIAAEIAAVAARPLADIARSDDYPLVGDDLVAAYVARAAS
                     LVPADAPRELNWVYTAMHGVGAAVVDAVVAACGFGEPARVSEQIAPDPAFPTVAFPNP
                     EEPGAIDLALALAREVSADVAIANDPDADRCAIAAVIDGDWRMLTGDELGALLADDLL
                     RQGVAGTFACSVVSSTLLGTMAAAHGQPFATTLTGFKWIGRVPGMAFGYEEAIGYCTD
                     ASAVPDKDGITTLVRVLALVARLRAASEAIANRLDEIARTYGLHLTSQLSFRVADLSL
                     ISDAMARVRSMPPSVLAGEAVSARDLASGSAELPPTDGMLFEGESVRVVVRPSGTEPK
                     LKCYLQVKLPVDASQDLDAARARAAEVMAAARQDMAAALGL"
     gene            21675..22238
                     /locus_tag="PKJ61_05860"
     CDS             21675..22238
                     /locus_tag="PKJ61_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002549896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HOC13382.1"
                     /translation="MLVAGPSGSGKSRLAHAAGVPSLRLDDYYFDADHPDLPKTDYGI
                     TDWDDPRCWDAAGAVAGLQELLATGRLVAPAYSISESRRVGTHEVRLGNAQCLTAEGI
                     FAIEFLAHCRAAGLQAEAIYLDRPAAMVFWLRLRRDLAKKRKPPLILFQRGWALCRAQ
                     PALKRKALAAGFTPMRMKAAITALTHT"
     gene            22508..23497
                     /locus_tag="PKJ61_05865"
     CDS             22508..23497
                     /locus_tag="PKJ61_05865"
                     /inference="COORDINATES: protein motif:HMM:NF024734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HOC13383.1"
                     /translation="MPVSSAQTAQQDQRVHRSGPKPNQLQSLAMEQSDLVTIQQVDDS
                     GVSRKTVNRLVRDGHWQRRRNGLFDIAPGHESLLKEVWTAALQAGTPYAIGGAAALWL
                     HGLDLRVDRIVIWVPADRRPRPPTSAIIKRDHLGRVDRGRGLPVRIRVEDALIDVGQN
                     LEVEGLVGVLSDATRLRLTTLRRVRQTLDERRRVRHRTDFKDILDDLTGIESNLEYVY
                     REDVERAHRLPAGNRQDRLSAGTRTDVTYDEQSLLVELDGRLGHEDSFSSFRDLHRDN
                     AHSLLEYRTLRYGSADVRGQPCAVALQVWQAMRLRGWQGPLSPCPSCPSEART"
     gene            complement(23627..25411)
                     /gene="metG"
                     /locus_tag="PKJ61_05870"
     CDS             complement(23627..25411)
                     /gene="metG"
                     /locus_tag="PKJ61_05870"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015071117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOC13384.1"
                     /translation="MSSRILAAVAWPYANGPRHIGHVSGFGVPSDVFSRYQRMAGNQV
                     LMVSGTDEHGTAIQVQADKEGLTPQQTADKYHRVIAEDLQALGCSYDLYTRTTTLNHR
                     AVVQELFRALHANGYVTAKVQQGAISPSTGRTLPDRYIEGTCPVCGYDGARGDQCDNC
                     GRQLDPIDLIRPLSKINGEVPNFVETEHFFLDLPALAGALGEWLATRTDWRPNVMKFS
                     LNLLKELRPRAITRDLDWGVPIPLEGWSDASMKRIYVWFDAVIGYLSAAVEWARRSGD
                     PDAWKLWWQSAEARSYYFMGKDNITFHSVIWPAILLGANGEGDLGGVGSAYSPLNLPT
                     EVVSSEYLTMSGSKLASSRGHVIYVRDFLAEFGPDALRYFIAVAGPENQDTDFTWEEF
                     VRRNNFELANEWGNLVNRSISMAHKNVGAIPVAGELTDADRELLSASAAAFDVVGDLW
                     GRCKFKQATSEAMRVVGLANKYLSEQEPWKLKDDPVRRDTVLHVALQVVSDCNTLLTP
                     LLPHAAQKVFEALGGEGVWAAQPELKLVSEEGGAEYPVLTGDYTCQQARWASAPIEAG
                     RPLQKPTPLFTKLDETLGQTGPTWAPIA"
     gene            25565..26791
                     /locus_tag="PKJ61_05875"
     CDS             25565..26791
                     /locus_tag="PKJ61_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017934146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HOC13385.1"
                     /translation="MTPEFLLLSLVVVTALAFDFTNGFHDTANAMATSIATKALTPKA
                     AVTLSAILNLVGAFLSVEVAITVTNAVIRIQNPDGTPKPELMADGGHGLLLILMAGLA
                     GAIIWNILTWLWGLPSSSSHALFGGLVGATVAAMGWGGVKWVGEGAKLDGVIGKVVLP
                     ALLSPAIAIIVAMIGTKLVFWVTRGVAEKYQESGFRWGQIGTASLLSLSHGTNDAQKT
                     MGVITLALIASGHWTATHEVPLWVKIACAVAIASGTYIGGWRIIRTMGKGLVEISSPQ
                     GMAAESSSAAVILTSSQLGFALSTTHVCTGSILGSGLGRKGAKVRWGVAGRMGLAWLT
                     TLPLAGLVGAVMWFIGHIIGGWLGSTTIFLLMCAAALYMRWHSKKDPIGHHNVNDEWD
                     AEQPDADKVPVSGGVH"
     gene            26791..27099
                     /locus_tag="PKJ61_05880"
     CDS             26791..27099
                     /locus_tag="PKJ61_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13386.1"
                     /translation="MDQLFAALDASWRVLLVGVVLGAGLPTIYALGIRALAWGTGGEA
                     EVHEAGVELKPHLLGRLIAYSLFAFVVLCVLLGIGYIVAHGLGMTITFDGIVPVFTSK
                     "
     gene            complement(27086..28114)
                     /locus_tag="PKJ61_05885"
     CDS             complement(27086..28114)
                     /locus_tag="PKJ61_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13387.1"
                     /translation="MTVLALVESPAQLLHVLEWCHRHAAAERIRLVVLAPVDERTRVQ
                     LAAMADYAGEENIAVEWHDPRTALSASARVIAELRPQVADAHTLLLGDPFSGLIQALL
                     PRPGNQQVVVVDDGTATLEFAAQLAAGERLRRWHGRSFGPVRAVLAERARRFFDTARL
                     QLFTVMAVTGLPPSRVQRHSYAWTRHRFGQVRTRPGVDVIGSSLVETGVVRPGAYLDA
                     VAALAIDGSAGRYFAHRRESAEKLDRIATQTGLEIVRPSVPLEVELRRTPVAARLASF
                     PSSVGYTLPLVLAGSGVRISVQPIPEEMFAEGVTQRARSFLARLGDDVRTTARVAGRA
                     RVELATLT"
BASE COUNT         4546 a         9836 c         9388 g         4455 t
ORIGIN      
        1 ttagcggccg cggaccgcct cgcccttggc cagggcggcc tcccgcaggg acgcctggaa
       61 cgcgaccatc tgctcggcca acaccggatc ggcggaagcc agaatccgca ccgccagcag
      121 gccggcgttg cgggcgttgc cgatggcgac cgtggccacc ggcacgccgg ccggcatctg
      181 cacgatcgag agcagtgagt ccatgccgtc caggtacttc agcggcaccg ggacgccgat
      241 caccggcagc ggcgtgagcg cggcgagcat gccgggcagg tgggcggcac cgccggcccc
      301 ggcgatgatc accttcagcc cgcgggcgtg ggcctcgcgc ccgtagcgca ccatgtcctc
      361 gggcattcgg tgggccgaga ccacgtccgc ttcgtacgga acattgaatt cagtgcaggc
      421 ctcagccgcc gccgacatca ccggccaatc ggagtccgag cccatcacga tgcccaccag
      481 tggctcactc atcgatcaat cccctcagat agtcggcggc agcatgcgcc ctccgccgcg
      541 ccgcggccaa gtcgtcgtca aggacggtca cgtggccgac cttgcgtccc ggcacgacgt
      601 ccttgccgta aagctgcgga cgcaggtact cgtcgcgcgc cagcacgtgc ccgagcccgg
      661 ccagcagatc ggtgcgcgtg ccaccgagga tgttcaccat caccgtccag cgcgaatcct
      721 gttcgggcga gccaagcggc agatcggcga ccgcacgcag gtggttctcg aactgggagg
      781 tggcggcacc atcgatcgtc cagtggccgg tgttgtgtgg ccgcatggcc agctcgttga
      841 cgacgatccg gccgtcacgt gcctgcatca gctcgaccgc aaggatgccg accacgtcaa
      901 gctcggtggc gatccgctcg gcgatctcga tcagccccag ggcgtccgcc ccgtcctcga
      961 accaggccgg agtagtggtc tcagtgcaga taccgtcggt ctgcacggtc tcggtgatcg
     1021 tgtagccgac catctcgccg ccgggacgcc tggccacgat cgcgctcagt tcgcgcgtga
     1081 agtcaatgaa ttcctcagcg atgatctgga cgccttcgcg cagcccggcg aacggcgact
     1141 cggcgtcccc tggaccgtcc agcttgaaca ctcccttgcc gtcatagccg ccccgggagg
     1201 tcttggcgat caccggccag ccgtgccgct cggcgaactc gaccagcgcg gacgcgtccg
     1261 gcaccacttc gaatgccggg cagggcacgc cgagcgcgct caaacgttcg cgcatcacga
     1321 ccttgtcctg tgcgtgcagg agcgcgtcgg gcccgggccg gacggcgatg ccgcgatcca
     1381 tcagcgcgtg caggatggcc ggcgggacat gctcatggtc gaaagtgatc acgtcgcagc
     1441 cctcggcgaa cgcgagcacg gtctcgggat cggtgtagtc acccacgacc acttcacgca
     1501 ccacctgggc cgcggagacg tccgggccct cggcgagcag ccggatgcgc agcccgaggg
     1561 cgatggacgc ggcgtgcatc atgcgggcca gttggccgcc gccgatgatg ccgatggtcg
     1621 tcgcgggact agtcacgcga tcgagcctaa aggaagcggg taccgtcccg ccggtcgggt
     1681 cgttctgcca agatgggcgc atgactgcgc atatcgtcgc aatgggtggc ggcggcttct
     1741 cgatgtcgcc gctgggagcc ccaacgaacc tggatcgcta cctggtcgaa ctgaccggca
     1801 aatcctcgcc gctggtgtgc ttcgcgccca ccgcttcggc tgacgatccg cagtacatca
     1861 accggttcct gctggcgtac ggcaccttgg gcgtgcggac gatgatcctc acgctctggg
     1921 agggcggctc ggccggctcg gtggcccggc tggcgaacgc cgacctgatc ctggtgggcg
     1981 gcggccacac ggtgaacctg ctcgcgctgt ggcaggcgca cggcgtggac aaggcgatca
     2041 aggagctaca cgctgccggc gacgtcgtgc tcgctggagt atccgcaggc ggctcgtgct
     2101 ggtacgaggg ctgcatcacc gactctttcg gcgacttccg gccgtggcgt ggcggcctgg
     2161 ggctggtgcc cggaagcttc tgcccgcact tcgacggtga ggccgagcgt gcccccacgt
     2221 tcacggacgc gatcgccggc ggcgacctgc ccggtggcta tggcgcggac gacggtgccg
     2281 gcattcacta tgtggacggg gtcgcgacct acttcatcag cgaggctcgc ggcaagcggg
     2341 tctaccgggt gctgccgtcc gacctgccca ccgcgtccgg ggtgctggtc gagccgcagc
     2401 agatgacgat tctctaggac gtcttgatgc cgctgggcag gaggcctctg ctcagcggaa
     2461 gatcaggccc cgctgcacga tgaagttcac cgtggtggct acgccctggg cgatcacgaa
     2521 gccgatcacc tgagcggcgg tctggcttgc ggtccagctc agcaccagcg ggaagagcca
     2581 tgcgaacagg cccacctgca cggcaaaggt aaggccgtac agcaccacca ccgcggcgaa
     2641 ccggcgcttt gacggctcgg cctggaacgt ccagcgccga ttgatcaggt atgccgtggt
     2701 cgtgccggcg atgaacgaca gcgcttttgc gggcgtgtgg tccagtccgg cgaggttcat
     2761 caacaccacc agcaggccga aatcgaccac tgctgacagc ccgccggaga tcacgaaccg
     2821 caccagctgg gtgcgcagcg ccaacctctc agcggctggt tcggtgccgg gctcggcctt
     2881 cgtcaccggc gaatcactcg tccttcaacg tcggacgcct gatcggatcc tcaccgccga
     2941 aaagcagctc cgtcatcacc acgtgcaccc gctcaacgtc cggaatgtcg ttcagtgtca
     3001 ccggctgttc ggccgcagtc gtcatcacca aagtgccgca accgagcagc tgatcgatcg
     3061 gaccgcggcg gtacgagacg ttgttgatcc tggccagggg caggtcgtgg cccttcttca
     3121 ccaggatgcc gctgcgggtg atgatccgcc ggttcgtgaa cgtgtacgtc gtggtgaacc
     3181 aggacaggaa cggcgccacg acccagatcg ccagcaccac gagcatcgcc agggcgacga
     3241 tccaacccgc cgccggctgc cagtccgcag gcagcagcgc gaacaggaag ccgctcacca
     3301 ccgcggtcac caccagcacg accgcgggca ctagcagcgc cttcacatgc gtgcggaggt
     3361 gcagcacctg cacctcatcc ctgcccagtt gttcagccgg caatcccatg cggcaatcct
     3421 gacacacggc ttcgctcgca gaacacggag gattctgcac cgtactcggt ggacaatcct
     3481 ccgcggcaat cgccgaccag cctcagcgca gatgaaacac gtcgccggcg gcgaaggcac
     3541 gctcgcgtcc gtccgactcc accagaaggc agccggacgg gtcgacgccg gtcgccctac
     3601 cggtgaccgc ctcatgctcg gaaagctgga cgcgcacctc ccggccgacc gaagtgcagc
     3661 gttcggcgta gtcagcgcgc agatcggcgc cggccagcca gcgccggtac caggcgtcca
     3721 gtgccagcag gacggacgcc gccacctcac cggtttccac ctcagctcca gcgagcgcca
     3781 gcgacgtcgc ggtcggcacc ggcaactcgt cttcgcgaag cgtggtgttg atgcccatgc
     3841 cgatgatcac cgccggcagg gtttcgccat gcccgaccgt ccggttcttg ggcgattcca
     3901 cccgctcggt caggatgccg cacaccttcc ggcccccgat cagcacgtcg ttgggccact
     3961 tcagttcggc agccaccccg caggccgcac gcaatccgtc cgccaccgcc agtccggcga
     4021 ccagcggcaa ccacagccag cgggccagcg gcaccgaagc cggcggacgc agcagcgccg
     4081 agatcgccaa cgaggttccg ggcggagctt cccatacccg ggtgaaccgg ccgcggccag
     4141 cgctctggtg gtcggacacc agcaccgcgc ccgttgccgc gccggcccgg gctgcggctg
     4201 ccagttcagc gttggtcgat ccgatggtgg cgaccgtggc caccgagccc cacgctccgt
     4261 ccgctgaaac gatgcgtctg gcaagccaat tcgcatcgat agtgggattc ccacccttgc
     4321 catctgccgg gcgcatagcc acaacgctat cgtctgctca tggagctgga gattcacacg
     4381 acccgtggca agatcgccca actcggcgaa cgaatcgagc aggccaagaa cccctcgccc
     4441 caggcttcgg tggagaaggt gcacgcgcag ggcaaaatga ccgcccgcga acgcgtcctg
     4501 cagctgcttg acgaaggcag cttcaccgaa ttcgacgagt tctcaagaca ccgatcgacc
     4561 ctgttcggcc tgcaggagaa acgtccctac accgacggcg tgatcaccgg cgtcggctcg
     4621 atccacggcc gtccggtctg tgtgttcagt caggacgtca ccatcttcgg cggcgcgctc
     4681 ggcgaggtgt acggcgagaa gatcgtgaag atcatcgact tcgcgatcaa gaccggctgc
     4741 ccgatcatcg gcatcaacga aggcggcggc gcgcgcatcc aggagggcgt ggtctccctc
     4801 gacctgtaca gccagatctt ccgccgcaac acgctcgcgt ccggtgtcat cccgcagatc
     4861 tcgctgatca tgggcgctgc ggccggcggc cacgtctact cccccgccct caccgacttc
     4921 gtgatcatgg tcgaccagac gtcccagatg ttcatcaccg gaccggccgt catcaagacg
     4981 gtcaccggcg aggacgtttc gatggaggac ctcggcggtg gccggacgca ctccaccaag
     5041 tctggcaact cccactacct ggccgcggac gagaacgacg ccctggaata cgtgcgccag
     5101 ctgatcgcct tcctgccaca gaacaacctt gaggacccgc cggtcttcga ggaggaggaa
     5161 gccgacctga cgatcaccga gcatgatctg aagctggaca cgatcgttcc ggattcgccg
     5221 aaccagccct acgacatgca cgaggtgatt cgcggcgtcc tggacgacga cgagttcctc
     5281 gaggtgcacg ccctctacgc gcagaacatc atctgtggct tcggacgcgt cgagggccgc
     5341 tcgatcggcg tgatcgcgaa ccagccgatg gttttcgccg gctgcctgga catcaaggcg
     5401 tcggagaagg ccgcgcggtt cgtccgcacc tgtgacgcct tcaacatccc gatccttacc
     5461 ttcgtggacg tgcccggctt cctgcccggc accgaccagg aatgggacgg gatcatccgc
     5521 cgcggcgcca agctgatcta cgcctacgcc gaggccaccg tgccgctgct caccgtgatc
     5581 acccgaaagg cctacggcgg cgcgtactgc gtcatgggct ccaaaccgct gggcgccgac
     5641 gtgaaccttg cctggcccac cgcccagatc gcggtgatgg gcgccgaggg agcggtcaat
     5701 atcctctaca agcgcgaact ggccgcagcg gacgatccaa cttcccgccg cgaggaactg
     5761 atcgacgagt acaacgacga gctcgccaac ccctatgtcg cagccgaacg cggctacgtc
     5821 gacaaggtca tctacccgca cgaaacccgt gcccaggtga tccgcgtcct acggctgctg
     5881 cgcaccaagc gtcagcagtt gccgcccaag aagcacggga acattccgct gtgagcaccg
     5941 agcccgagca gctcgtccag atcgtcgctg gccagcccac cgacgaggaa ttggccgccc
     6001 tcggcgcggt gatcgtcgcg ctgcggcgcg cgcgtcccga gccccacgcc ccggtgacca
     6061 atacccttgc cggcggctgg cgctcctact ggcatacagt gcgtaaccca ttactgcccg
     6121 gccgtgaggc ttggcggagt acttttagga gttgagcggt cgagaaacgc agcccgtcgc
     6181 taggaaagga ctcgaaatga gtgtcatcga cgaaatcctg ggcagcatcg accccgagca
     6241 gctcgctgcc gctgtcggga ctgaccagca gaccgccatg gacgcggccg ctgccgcgat
     6301 cccgaccctg atcaacagcc tgcaagccaa cgcaaccgcg tcggcggacg gtgaagccag
     6361 cctgctgcag gccctcggcc agcacgccga cggtatcttc ggcgaccagg tcgacctggg
     6421 caatgtcgac accgaggacg gacgcaagat cgttgcgcac gctctggccg acgacccgca
     6481 gcgcctgcag gctctcggtg gtttcggcgg tgggctgctc agcaagctgc tgccgctgct
     6541 cgcgccgatc gtcatgagct acctctccaa gaagctgctc ggcggcggcg cctcctcggg
     6601 cggctcgggc aacgtcctcg gtgacctgct cggcggcatt ctcggcggtg gcgccaccca
     6661 gcagtccgcc ggtggcgggc tcggcgacct gctcggctcg atcctcggcg gaggctcggc
     6721 acaacagtcc tccggcggcg gcctgggcga tctactcggc tcggtgttcg gcggccagga
     6781 gccggccgca gtgccggccg gcgtgggcgt ggataccagc ggccaggtct tcaacgcgcc
     6841 ggacggctcc atgcagatca acaccgacgc acccgtccag acctacgagc agcccccgca
     6901 gcagcagcaa cagggtggcg acatcctggg tgacctgctc gggcagatcc ttggtcgctg
     6961 attcgtcacc ggccctggtg ctggcctccg cctcccccgc gaggttggag accctcacca
     7021 gggctggcct acgtccgcag gtgatcgtct ccgcggtgga cgaggacgcc ttcaccgcga
     7081 ctaaggtgac cgagttggtc gcggtgctgg ctcaagccaa ggccgaagct gtcgccgcag
     7141 tgctgccgga ggatggggac gtcctggtgg tcgcctgcga ctcgttgctg gacctggacg
     7201 gtcgaccgct cgggaagccg gcgacaccgg aagaggcgat ccagcgttgg caggaaatgc
     7261 gcggccgcac cgggcacctg cacaccggcc accacgtgat cggacgcctc gacggccaga
     7321 cccggtcggt cgcagcggtg gccaccaccg aggttcgctt cgcggacgtc accgacgccg
     7381 agatcggggc ctacgtggcc accggggaac cgcagcaagt agccggcgcg ttcaccatcg
     7441 acggcctcgg cggagcgttc gtcaccggta tcaccggcga ccaccacaac gtggtcggca
     7501 tcagcctgcc actgctgcgt cggctgttcg ccgaactcgg cgtcggttgg cacacgctct
     7561 gggcctagtc gatcctccga cccggctgtc accggctcca agatcaacgt ggacagctcc
     7621 gcgcccggat gccgccaagg cgtccactct gcgccgaagc cgcactcgaa ccagtccccc
     7681 caatcaaggt ggacgattgc gcgccgtcac gacgagtacc cgtccacctt gtctcgaagg
     7741 tgagtccacc agcttccgcg caagtcagcg tcgatgtggc gagagacagc ccgtcgacgg
     7801 agtgtcagtc gtgcccggta gccttgtgcc gctatgtcga atgcgaatct gcctgaagtg
     7861 gccccgaagg ccgcactgct tcccgcctgg ttgcgggcga tgcggcccaa gcagtgggtg
     7921 aaaaacgtcc tggtgctggc ggctccggtc gcggccggcc ggctcttcga aaccccggtg
     7981 ctgatcgcca cgctgtgggc cttcgtcggg ttcagcctgg tcagcgcgtc catctacctg
     8041 atcaacgaca gccgcgacat cgaagccgac cggctgcatc cgaccaagcg cttccggccg
     8101 atcgccgcgg gcgaactcag ttcgaacctc gcctacgtgc tggccgcgat caccgctgcc
     8161 gccgcgttcg ctatcggctt ctgggtggcg ccgctgctcg gcgcgaccct cgccttctac
     8221 tgggtgctgc aggtgctgta ctcactcttc ctcaagaacc agcccatcat cgacctggcc
     8281 atggtcgcgg ccggcttcct gctgcgcgcg gtggccggcg gcgtcgcgtc cggcatcgaa
     8341 ctgtcccagt ggttcctgct ggtggcctcc ttcggctcac tgttcatggt ggccggcaag
     8401 cggtactccg agctgagcga actcggccac aaagccggca cgcgggccag cctggagcgc
     8461 tacaccaagt cgtacctgcg cgaggtctgg tcggtctcga tcgccgtggt gatcatgtcg
     8521 tacagccttt gggcgttcga acagggcggc ggatcggcct ggggcgtgcc ctgggctgcg
     8581 atctcgatcg cgccgttcac ccttgcggta ctgcgctacg ccatgaagat cgatcagggc
     8641 aaagccgggg aaccggaaga ggtcgttctc ggggatcgga tgctgcaggg gctcgccgtg
     8701 gcctgggtga ttccgctggc gatcgcggtg tttgcatgag catcccggtg aaccgtccgg
     8761 tcgcgttggt caccggcgcg tccaagggaa tcggacgcca aatcgcgctc gcattgtcgg
     8821 ccacgcacca cgtgctggtc ggtggtcgcg acgccacccg ggtcgccgcc gtggtggctg
     8881 aactggagtc agccgagcca ttcctggccg agttgaccga tgcagcagcg gtcacggcag
     8941 cagtagccgg aatcgacaaa ctggacgtgc tggtgcactc ggccgggctg gtcgcctacg
     9001 gcccgctggc ggagtcgacg accgatcagt ggcgcgcact gctggaactg aacgtgatcg
     9061 ccccggcgaa cctcacccgg ctgctgctgc ctgcattacg cgcggcgacc ggcctggtgg
     9121 tgttcatcaa ctccggcgcc ggcctgaccg cccatccggg attctccgcg tacgctgcgt
     9181 ccaagttcgc cctgaccgcg ctggccgatt cgctgcgtgg cgaggagcgc ggacgcgtcc
     9241 gggtcacctc gcttcacccc ggacgggtgg ccaccgacat gcaggccgaa ctcgccgccc
     9301 tcgaaggtcg cgtctaccac cctgaggtgc acctcgaccc ggcagcggtg gcccgcgccg
     9361 tccggctggc cgtggagctt ccggacgcag ccgtggacgc cgtccggatc aaccccgcct
     9421 agtgctccag cccgagtacc actgagaaac cgagcatggc ggtgccggcc gcggcgtcga
     9481 tgatccgcgg gccgcgtcca gcggtgagga agccgcgtaa cgcgttcgca ccgaggatca
     9541 cgacggtgaa ccagaccatc ccgagcaccg cgtgaatacc ggccagcagt gcgccgatgg
     9601 ccgctggcgg gtagccggcc ggtaggaaca gcgggagcac cgtgacgtag aagacggcga
     9661 tcttcgggtt cagcaggttg gtcagcaacc ccttcatgaa cgcctcgcgg acggtgtccg
     9721 tgccgccgcc ggaggtgtcg aagtgcgaat cgccgcggat cgccgcccgc agatagctgt
     9781 aggcgagata gagcaggaag gcgattccgg cccatttcag caccaggtag gcagtctggg
     9841 acgcgacgaa caacgcagcc aggccggtgg cggcggcgac tccccagacc agacagccca
     9901 cacagatgcc gaggccggcg gcgaaagcca ccgcgcggcg gccacgcaag gcctggcgca
     9961 gcaccaggac ggtatccagc ccgggcgtga ccgtgaccac ggcagccagc agggtgaagc
    10021 cgagcacggc ctgcgtcaag gtcatttgcg aatgctaacc cccctcgctt ccccctatcc
    10081 aagccttcgc catccaaccc accagtcgtt tgcgagcggg agccgcgccg gagccgagct
    10141 atcggacggg cgcctggcgg ctcggtcaga tccagtcgac cttgcggaac aggatgtaca
    10201 agccgattga gccgaccagg atcagcgcgg cgctctgcca gagtccccat tcggccatgt
    10261 agcccgggta gggcaggttc tggccgaacc agcccgtgac cgcggtcggc accgcgatga
    10321 tcgccgccca cgcagccagc ttcttcatca cctcgttgag ccgggcgtcc tgaagcgaga
    10381 ggttggtctc aaacacgctg gtgaccatgt cgcgcagact ctcggtccat tcggcggccc
    10441 ggatcacatg gtcgtagagg tcgttgaagt ggccggcgag ctccggcgag gtggcgcgat
    10501 ccccttcggt gtggaaccgc cggacggtgg tgacgacttc gcgcatcggc agcaccaccc
    10561 gccgcaactg caccagcgcc ttgcgcagcc cgaacactcg ttgctggacg acacgggtct
    10621 tcatcgccgg gtcgaagagc aggtcttcca ggctctccag catgtcgtcc atggtctcga
    10681 tggtgtcgaa gtgcccgtcc acgagctggt cgagcaggcc gtgcaccaac gcggccacac
    10741 cgttcccgag caggccgtcg tccgtccacc gggccaacgc gtcgtccatg tccacccgat
    10801 cgtccgggcg cacggtgatc aacgcgttcg gcagcacgaa ggcggaaacc cggccgagcg
    10861 ccagctccac actggccccg tccagacgcg cgaagccagt cgcgtagcag gtgaggaaca
    10921 cgtgggtcgc atgcatggac gccttgggcc gctcgcgcgg ttcgaccgcg tcctccaccg
    10981 ccagtgggtc aaggctgagc tctgcagcga gcgtgtccaa aagggtgcga tcgggctgga
    11041 gcaggtcgaa ccagaccagc acaccgggag cggccagcca ctggtccagc tcggtcagcg
    11101 gaaagtcggc tgcgatctcg acgccgtccc gccaggcgcg gctgtggcta ctggtcactg
    11161 gatcagcatt gcaccggcgc cgcacccagc gacgtaaccg gcgccggttt ggccgccaga
    11221 acgtcacacc agtcctggcg gccgggagtt gcgtccactc cgcagatggg tgcgcgaatc
    11281 cgtcccgctg ttgcgggctt ggtcactaga ctccgcaaca gttgacagca gggtttccag
    11341 gaggagtcaa tgccgatcac caaagtgctt gtggccaatc gcggcgagat cgccgtccgg
    11401 gtcgtccggg cagcgcgcga cgcgggcctg ggcagcgtcg ccatctacgc cgacccggac
    11461 gcggactcgc tgttcgtgaa gctggcggac gaagctttcg cactgaacgg gcaaacgccg
    11521 gccgatacct accttgatgt cgcgaagatc ctcgagatcg ccgcgcgttc cggggcggac
    11581 gccgtccatc ccggctacgg cttcctcgca gagaacgcga acttcgccca ggccgtcatc
    11641 gacgccggac tcacctggat cggcccgccg cccgcggcca tcgacgcgct cggcgacaag
    11701 gtgaaggcgc gccacatcgc gcagaaggtc ggggcgccgc tggtgcacgg cacgcccgac
    11761 ccggtcgccg gagccgacga agtggtcgcc ttcgccaagg agtacggact gcccatcgcg
    11821 atcaaggccg cctatggcgg tggcggtcgc ggcctcaagg tggcccgcga tctcggtgag
    11881 gtcgccgagt tgttcgagtc ggccacccgc gaggcggtca ccgctttcgg tcgcggcgag
    11941 tgcttcgtcg agcagtacct cgaccgtccg cggcacgtgg agacccagtg cctggccgac
    12001 gcacatggca acgtcgtcgt ggtttccacg cgggactgct cgctgcagcg tcggcaccag
    12061 aagctggtcg aggaagcgcc tgcgccgttc ctgaacgacg agcagatcga gattctctac
    12121 acggcttcga aggccgtcct gaaggaggcc ggctacatcg gcgccggcac ctgcgagttc
    12181 ctggtcgccc aggacggacg gatctccttc ctcgaggtga acactcgctt gcaggtggag
    12241 cacccggtgt cggaggaggt ttcgggcatc gacctggtgc gcgagatgtt ccggatcgct
    12301 gacggcgaag agctcgggta tgccgacccg tcgctgcgcg ggcactcgat cgagttccgg
    12361 atcaacgcag aggacgccgg ccgcaacttc atgccggctc cgggcacggt gaccgcctgg
    12421 gtgcccccga ccggacccgg cgtccgcgtc gattccggct acctggcggg catggccgtg
    12481 ccgggctcgt tcgactcgct ggtggccaag atcatcgtca ccggcgcaga ccgtccgcag
    12541 gctctggccc gggcgcggcg cgcgctggcc gagaccacgc tggaaggcat gccttcggtg
    12601 atcccgttcc acaaggtggt tctggacgac ccggcatacg tggccgcgga cgggcacttc
    12661 ggcgtgcaca cccgctggat cgagaccgag ttcgtcaacg agatcgcgcc gtacgtgggc
    12721 gaaatgggcg aggccgagga gaccgagaag aacacctacg tggtcgaggt gaacggccgt
    12781 cgcctcgaag tgcggctgcc gagttcgctg gccgcgccgg tggtgaaggc cgccacggcg
    12841 aagcgtccgg tcaagcggag cacgggcggc gcgaaagcca aggcgccctc ctcgaacgag
    12901 ctgtccgccc cgatgcaggg cacgatcgtg aaggtcgcgg tgatgaacgg cgacgaggtc
    12961 aacgagggtg atctcgtcgt ggtgctggag gccatgaaga tggagcagcc gatcaccgca
    13021 caccgcgccg gcatcgtggc caacctgtcc gccgccgtcg gcgattcggt ggcctccggc
    13081 caggcgctgc tggagatcgt cgacccgacc tgacgctgct tccggctgag gtagcgggcc
    13141 ggtgctgccg tgtcagcaag gttcacgcca gcgatccgga cgtttgccgg tgaatgttcc
    13201 ccggcaagcg tccggatcgc tggcgtgaac cttctggcgt gaaccttgga gacctaccgc
    13261 gctacaggta gcgggacgcg gccgcggcgt actgctcgct gatctcggga cgcgggtcgg
    13321 gcgcgggcgc gccgatcagc tcgaccggcc aatccgggaa cgctccaccc agtatgccgg
    13381 cggcttggcg cgcggcaccg tcggccacgt actcacccgg cgagggcatc tcgacggggg
    13441 cgtcgaagac ctgtgcggca acggcctgca ccgccgggct cgccgcggcg ccgccgatca
    13501 gcagcacccg gtcgatccgc gcaccttgcg cccggacggc gtccaagccg gcaccgagac
    13561 cgccgagcat gccctcgacc gcagccctgg ccagattcgc gcgggtggtg ttggccagcg
    13621 tgagcccgga gagcgtggcg gtcgagtccg gaaggttcgg cgtccgctca ccctcgaagt
    13681 agggcaccag gaccgctccc ccggcgcccg gcccggcgtc cagggccagc cgggccaact
    13741 cgtcgtggtc gacacccagc agtccggcga ccacatccag cacgcgcgcc gcattgagcg
    13801 tgcagaccag cggcaggaat ccgccggtgg cgtccgcgaa gcccgcgacc gcgccggacg
    13861 cgtccgctgc cggcacctcc gccgcggcga agaccgtgcc gctggtgccg agcgagacca
    13921 ccacgtcgcc ggcgcgtgca cccagaccga gcgcggcacc ggcgttgtca ccggccccag
    13981 gaccgacgag gaaaccgggg ccgggcacgc tctccagggg cgccagcacc cgcggcagca
    14041 gcgcttcgtg gcccagcgct cgcaccaaga ggtcgcgatc gtaggcgtcc gaggacggcg
    14101 accagtagcc ggtgccggat gcgtccgacc ggtcggtgac cagctcttcc agcaccgggc
    14161 caaggtccga ttccgaagcc gggccgtacc cgcgcagtcg ccaggtgagc cagtcgtgcg
    14221 gcaacgccac cgcagccgcc cgcgccgcgt tctcgggctc ggcgtcccgc agccagcgca
    14281 gcttggtgat ggtgaacgag gccaccggca ccgaacccgt ccgacggacg aactcgtccg
    14341 cgccgacttc ggcgatcagg tcgcgggcgg cgccggccga gcgggtgtcg ttccacagca
    14401 gcgccggacg gatcacccgt ccgtccgcgt cgagtacgac catgccgtgc tgctggccgg
    14461 cgatgctcac cgcggcgatg tcgtccaagc cgccggcctg cgccagtgct gcctgcaacg
    14521 ccgtccacca gtgagcgggg tcgacctcag acccggccgg gtgcgacgcc cgtccggacg
    14581 cgagcagcgc ccccgactcg gagtcgcgca ccacgacctt gcagctttgg gtcgaagagt
    14641 cgaccccgat cacccgtgcc atggctcagc gggcgccgag caggtgttcg gtggccagct
    14701 gctgcagccg gacgaagccg aagcccttgc cacccaggta ggcggccgag tcgaactcct
    14761 cgtaggcgga cgtgtccgcg agcagctcgg cgtagccctc accatcgccc aacgtgggca
    14821 ctgcgagctc tgtgaccttg gccgcagcca gcgcttcctg cacctccgga tcggcgcgga
    14881 aggccgcggc gcgctccttg agcagcaggt aggtgcgcat gttggcctgg accgaatccc
    14941 agacgccggt ctcgtcctcg gtgcgcgagg gcttgtagtc gaagtgccgc atgccctcgt
    15001 acgccggtcc gccgttggga ccgccgttct ccaggaggtc gaccagcgcg aaggcgttgt
    15061 gcaggtcgcc gtgcccgaag accagatcct ggtcgtactt gatgccgcgc tggccgttca
    15121 gatcgatgtg gaagagcttg ccgtggtaga gcgcctgcgc gatccccgcc tggaagttca
    15181 gtccagccat ctgctcgtgc ccgacctcgg ggttcagacc gaccagctca gggtgttcga
    15241 gggtctcgat gaacgcaagg gcgtggccga gggtcggcag caggatgtcg ccccggggct
    15301 cgttcggctt gggctcgatc gcaaacttca ggtcgtaacc ctggtcgagc acgtactggg
    15361 ccagcaggtt cacggcctcg cggtagcgct ccagagccga acggatgtcc ttggcctggt
    15421 cgtactcggc gccctcgcgg ccgccccaca tcacgaaggt cttcgcaccc acctcgttgg
    15481 cgaggtcaag gttgcgcagc accttgcgca gcgcgaagcg gcgcacctcg cggtcgttgg
    15541 aggtgaagcc gccgtccttg aacaccgggg cgctgaacag gttggtggtg atcatcggca
    15601 ccttcaggcc ggtcgccgcg caggccgcct tcagccggtc gatctggttg cggcgctcgg
    15661 cgtcggtcga accgaacgcg aacaggtcgt cgtcgtggaa ggtgaggccg taggcgccgt
    15721 actcggccag cttctcgacc acgtgcacca cgtcgagcgg gtgccgggtc gggccgccga
    15781 acgggtcggt gccgttgtag ccgaccgtcc agaggccgaa ggtgaacttg tccgacgggg
    15841 ccggcgtagt agtcatcgaa agaatcccat ctctccgcag tgagtgaatg ttgtgagcca
    15901 caacatatct cttcgagagc ctgcttgtcc atcgttcgaa gcggcgaatc accgcgggcc
    15961 gactaccggg ggtcaccgtg cggcgagcgt gtcagcggtc cacatgtcga agagtctgct
    16021 taggttgcct tgaccgacaa agggccgtct aggccacgtc gaggagagga tgcaatgcca
    16081 gcagtgacca ccgagggcgt ccggcggagc aacctcgcgg cgaccctcgg catcctctac
    16141 caccacggcc cggcgtcccg cgcggctctg agcaagcgca ccggacgcaa ccgctccacc
    16201 atcgccagcc tggtcgccga cctggtggag ctgggtctgg tcgcggagag cgatcctgac
    16261 ccgatcggcc aggtcggccg ccccagcccg gtggtcagca tccgtcccga ggtgaccgca
    16321 atcgcggtga acccggagat tgacgcgctc acgatcggcc tggtcgggct ggacgggaac
    16381 gtccggcggg tgatccggca cgagtacgac cgcattccga ccgcgtccga gttgatcagc
    16441 atcaccgcag cggtcctggc cggcatgcaa ctcgagctcg atggcctgct ggtggtcggc
    16501 atcggggtcg cgctgcccgg ccaggtacgc actcacgacg gcctggtccg taacgctccg
    16561 cacctcggct ggcacgacga gccctttgct gagccgctcg ctgcggccac cggctatcgc
    16621 gtccgggtgg ccaacgatgc ggccttgggc gcactggccg agcattcttt cggcgccgga
    16681 cgcgggaaga gcaatctgat ttacctcaac ggcggtgcct ccggcatcgg cggcggcgta
    16741 atcgccggcg gtcggctact gggcggcagt gccggttacg cgggcgaact cgggcacatc
    16801 cgcgtgtcca gctcatcggt gcgcgactcc gccggcatcg ccgggacgtt ggaggccgag
    16861 gtcaccagag acgaactgca gaccgttttg ggcctggcca gggtcacgcc cggcgagttc
    16921 agcgaagcgc tgctggccga ccgctcggac gcagtggcca cggtggtgcg ccgccagctc
    16981 gggcatctcg gcacggccat cgcggccgcc atcaacgtgc tcaacccgca ggtcgtggcc
    17041 ctcggcggct tcctggccac cctggctgag tacgaccgtg tattacttga acgaacagtg
    17101 aaggacgcga ccctcacccc gtccatggag ggcgtctcga tcaccgcggc cgaactgggc
    17161 gaaaacctgc tcatggttgg tgccgcgggc ctggccttcg gcgaactgct cgccgatccg
    17221 gcgtccatcg cgcggtaagc cctaacctcg gctggtcgag cctgtcgaga ctccgcttca
    17281 gtagacgatg acctgctcgg acgagtgccc gtgcagccgc cgtgcagcct cggccagcga
    17341 gcccgacaac gacggataga ccgtgaacgc ctcggccaac tggtcgacgg tcagcttgtg
    17401 gcgcacggca accgagagca cgtggatcag ctccgatgcc gaaggcgcca ccaccactcc
    17461 cccgacgacg atccgcgtgg tcggcaggca gaacaccttc acgaaaccgt cgcgcagcgc
    17521 ctgcatcttg gcgcgcgcgt tcgatcgcaa cggcagcgtc atgatcaggg cgttcagctt
    17581 gccggcctca atgtcggctt ggctgactcc caccgtggcg atctccggcg cggtgaagac
    17641 attcgacgag accgtccgca ggtccagcgg cgccaccgaa tcccccagcg cgtgccacat
    17701 cgcgatccgt ccctgcatgg ccgccacgga cgccagcgcg aacaccccgg tgcagtcgcc
    17761 cgccgcgtag accccgcggg ccgatgtgcg tgacacccgg tcgaccgtga tgtagccgcc
    17821 gctgtttacc gtcaccccgg cctcggccag cccgatgctg gcggtgttcg gaatcgcgcc
    17881 cacggccatc aggcagtgcg aaccgacgac ttcggcgccg tccgccagcg tcaccaccac
    17941 ccgctcgccg cggacctcgg cgccaaccgc gcgcacctga ttcagcacgg tcatgccgct
    18001 gccctcgaag acgtcctgga tcaccgtggc cgcgtccagg tcctggccgg gcagcacctt
    18061 gtcacgtgag ctaaccaggg tcaccttcac ccccagcgcc tgataggcgc tggcgaattc
    18121 ggcaccggtg acgcccgagc ccaccacgat caggtggtcg ggcagttcgt cgaggtcgta
    18181 gatctgggtc caggtgaaga tccgcttccc gtccgggcgc gcggtgtcga gttcccgcgg
    18241 gtgcgcccca gtggcgagca gcacgatgtc cgcgtccagg gtctcgacgc catcggcgct
    18301 ctcggcgacg acgcgctgcg gtccgtccag ccgtccggtt ccgttgatga tccggacgcc
    18361 ctcggcgcgc agccgctcgg cgatgtcgtc ggactgcgcg cgggccagcc cacggatgcg
    18421 ggcgtggacg gcgcccagat cgatcccgac cgcggtgtcg aggtggcggg tgtcgtcgag
    18481 caggcgcagt cccaactcct cggcgctgcg gatcttcgac atcacctcgg ccgcggcgat
    18541 cagggtcttg gacggcacca cgtcggtgag caccgccgaa ccacccaggc cgcgccgctc
    18601 caccagcgtc acgttgccgc ccagttgccg gccgaccagc gccgcctcgt agccgccggg
    18661 tccgccaccg atgatcacca cgttcgtcac gccggaattc ttgcacgcgg cccgcgcacg
    18721 ttgtcggcct tggcggttag tctcgtccgg tgcctcgcat tcttctcgcc gcagtcgccc
    18781 tggccggcgt cgtcgtcagc gtcgccatgg tggtgatcgg cctgccggag gccaacgccg
    18841 tccagtcggt gctgcttgag ctgagcgccc tgccgctctc ggtctcggtg gccgcgggca
    18901 tcggcatcct gcacctgggt gctgagcgtc cgccgggccc gcgtggccgc gccgcgctgc
    18961 tggtcggcgc cgggctggca ctgctcgggc ttgccctgat cgtctgggca tacctggtcg
    19021 gcccgcgggc cgccgtccat cccggacagg tgctgatctg gctggggctg ttcgcagcgc
    19081 tcctggtgac cgtccgccgc cagccgaaac gtcgcctggt gacctacgaa atcgaagaag
    19141 atgccgacgg cacgtccacc gaagaggacg catcgttata gtcgtgtagg tgaccgaaac
    19201 cgacccaaaa tcgctggccc gatcggccgc gtccgcactg ctggagcgct ttggaataga
    19261 ggccatcgat ctggccctgg tgcttgggtc gggatggtcc ggtgcggccg tcggccttgg
    19321 tgaggcgatc ggcgaatgcg agctcgcgga tctgcccggc ttcgccaagc ccgtcgtcgc
    19381 cggccacggc ggcgccctca agctgatccg cacgcccgcg ggcaagacag ccgcgctgtt
    19441 caccggacgc acccatttct atgagggatg cggcgtcgag ccggtcgtcc acggcgtgcg
    19501 gaccgccgct gccgccggcg cgaagatgat cctgctgacc aacggctgcg gtggcctgaa
    19561 ccccgattgg gcgccaggga cgccggtgct gatccgcgac cacatcaacc tgaccaatgc
    19621 gtctcccttg gccggggcta ctttcatcga tctgaccgag gcatactcgc tgcggttgcg
    19681 cgagcttgcg cacgaggtcg atccgtcgct ggcagagggt gtgtacatcc agttccgcgg
    19741 gccgcagtac gagacccccg ccgaagtccg catggccaaa gtgctcggcg gtgacctggt
    19801 cggcatgtcg accacactcg agaccatcgc cgcgcgcgaa gccggactgg aggtgctcgg
    19861 catctcgctg gtgacgaacg ccgccgcagg catctccccc accccgttgg agcacgccga
    19921 ggtgatcgcc gccggcaagg ccgccgagcc gcgcctgcgc gggctgctcg ccggactcct
    19981 cgaacgcctc taggagagat catggacgca acgcttcgcg atcagctggc ggcctggcgc
    20041 gagcaggatc cggacgtggc cacggccgct gcccttgacg acctgctcgc tagggcggac
    20101 gccggtgaca ccgccgccgc ggcggaactg gcggacgcct tcgccggacc gctggagttc
    20161 ggcaccgccg ggctgcgcgg cccattgggc cctggcccgg cccggatgaa ccgggtagtg
    20221 gtcagccagg ccgccgctgg gctcgccgcc tacctgaacg cgcgcgggct gaccggcgga
    20281 cgcgtggtcg tcggctatga cgcccgctac aactccgaag tgttcgcccg cgacactgcc
    20341 gagatccttg ccggcgccgg gttgctgccg ttgctgtgtt cgcgtccgct gccgaccccg
    20401 gtggtggcct tcgggatcgg gcacttcgac tgcgttgccg gggtggtcgt caccgcgtcc
    20461 cacaacccgc cgcaggacaa cggctacaag gtctatctcg gggacggctc gcagatcatc
    20521 ccgccgaccg atgccgagat cgcggccgag atcgccgcgg tggcggcccg tccgctggcc
    20581 gacatcgctc gcagcgacga ctacccgctg gtgggcgacg acctcgtcgc ggcctacgtg
    20641 gcccgggccg cgtccctggt gccggcggat gcaccgcgcg agctgaactg ggtgtacacc
    20701 gccatgcacg gcgtgggcgc agcggtcgtc gacgcagtgg tggctgcctg tggcttcggc
    20761 gagcccgccc gggtgagcga gcagatcgct cccgatccgg ccttcccgac ggtggcgttt
    20821 cccaatcccg aggagccggg cgcgatcgac ctcgctctcg cactcgcccg cgaggtgagc
    20881 gccgatgtgg caatcgccaa tgacccggac gccgaccgct gcgcgatcgc ggccgtgatc
    20941 gacggcgatt ggcggatgct gaccggcgac gaactcggcg ccctgctggc cgacgatctg
    21001 ctgcgccagg gcgtggccgg cacctttgca tgctcggtgg tgtcctcgac gctgttgggc
    21061 acaatggctg cggcccacgg ccagccgttc gcgaccaccc tgaccgggtt caaatggatc
    21121 gggcgggtgc cgggcatggc cttcggttat gaggaggcga tcggctactg cacggacgcg
    21181 tccgcggtgc cggacaagga tggcatcacc accctggtac gggtgctggc cctggtggcc
    21241 cggctgaggg cggccagcga ggccatcgcc aatcggctgg acgagatcgc tcgcacctac
    21301 ggcttgcacc taacttcgca gctgtcattc cgggttgcgg acctgtcgtt gatctcggac
    21361 gcgatggccc gggtgcggtc gatgccgcca tcggtgctgg ccggcgaggc ggtttcggcg
    21421 cgcgacctgg cgtccggctc ggcggaactg ccccccacgg acggaatgct gttcgagggt
    21481 gagtcggtgc gcgtggtggt gcgtccctcc ggaaccgagc cgaagctgaa gtgctacctg
    21541 caggtgaagc tgccggtgga tgcgagccag gacctcgacg cggccagggc gcgcgcggcg
    21601 gaggtgatgg ctgcggcacg gcaggatatg gctgcggctc tgggcctgtg agtccgtccg
    21661 gccaaccaag gttggtgctg gtcgccgggc cgtccgggag cgggaagtcg cggctggcac
    21721 atgcggccgg ggtgccgtcc ctgcgcctcg acgactacta cttcgatgct gaccatcccg
    21781 acctaccgaa gaccgactac ggcatcacgg actgggacga tcctcgctgc tgggacgcgg
    21841 ccggcgcagt cgccgggctg caggaactgc tggccaccgg acgcctggtc gccccggcgt
    21901 actcgatctc ggagtcgcgc cgcgtgggca cccacgaggt gcgccttgga aatgcccagt
    21961 gcctgaccgc ggaaggcatc ttcgcgatcg agttcctggc gcactgtcgg gccgctggtc
    22021 tgcaggctga ggcgatctac ctcgaccggc cggccgcgat ggtgttctgg ctgcgcctgc
    22081 gccgcgacct ggcgaagaag cgcaagccac cgctgatcct gttccagcgc ggctgggcat
    22141 tatgccgcgc ccagcccgcg ctgaagcgca aagctctggc cgccggcttc accccgatgc
    22201 gcatgaaggc cgccatcacc gcgctcaccc acacctgagc cgccacccac ctgaccccgc
    22261 cgcagccccg cgtcgcgctc cggcccgcca cctctcgcaa actggacagt tctggcccgt
    22321 cctgggacgc atgtgtccac cttgcgaact cgccggcaaa cccccggcgg ccaccgtgcg
    22381 caagctggac agttggtgcc cgtcctggga cacatgtgtc cagcttgcgg cgggtgcatc
    22441 cctactcagg gtgcgtcacc taattcagaa ctgtggataa ccaaaacccc tatccacaat
    22501 tctttcgatg ccagtttcat cggcgcagac tgcgcagcag gatcaacgcg tgcaccgttc
    22561 tggaccgaaa cccaaccagc tacagagtct cgcgatggag caatccgacc tggtcaccat
    22621 ccagcaggta gatgacagcg gcgtcagtcg caagaccgtc aaccggttgg ttcgggacgg
    22681 tcactggcag cggcgtcgaa acgggctctt cgacatcgcg ccagggcacg agtccctact
    22741 caaggaggtg tggaccgcag cccttcaagc aggcacgccc tacgcaatcg gcggagcggc
    22801 cgcgctctgg ttgcacggac ttgatcttcg agtggatcga atcgtcatct gggtgcccgc
    22861 cgaccgtcgt ccgcgtcccc cgacgtcagc gatcatcaag cgtgaccatc tcgggcgcgt
    22921 ggatcgcggc cgtggactgc ctgtgcgaat acgggtcgaa gacgccctga tcgacgttgg
    22981 gcagaacctc gaagtcgagg gcttggtggg tgtgctgtcg gacgccacgc ggctgcgact
    23041 gacgaccctg cgccgcgtcc gccagaccct cgacgaacgg cgtcgagttc gtcaccgaac
    23101 cgatttcaag gacatcttgg acgacctgac cggcatcgag agcaacctcg agtacgtcta
    23161 tcgcgaggat gtggagcgtg cccatcgact gcccgcggga aaccggcagg atcggctctc
    23221 cgctggtacc cgaaccgacg tgacctacga cgagcagtcg ctcttggtag aactggacgg
    23281 ccggctgggc catgaggact cgttctcgtc gttccgcgat ctccatcgtg acaacgctca
    23341 ctccctgctg gagtatcgaa cgcttcgcta cggcagcgcg gacgtccgcg gccagccatg
    23401 tgccgtggcg ttgcaggtct ggcaggcgat gcgccttcga ggctggcagg ggcccttaag
    23461 tccgtgccca agctgcccga gcgaggcacg gacgtagtca aggcaagcgt catgactccg
    23521 gcccgacgcg ttcacgtcct gaacaagctg gagcgctgga tccccagacg ggccacaact
    23581 gtcccgtttg cggttggagg aggcagtggg cggacgagac ggcggctcag gcgatcgggg
    23641 cccaagtggg accggtctgg cccagggtct cgtcgagctt cgtgaacagg ggggtcggct
    23701 tctgcagcgg acgaccggcc tcgatcggcg ccgaggccca cctcgcctgc tggcacgtgt
    23761 agtcgccggt cagcaccgga tactcggcgc caccctcctc ggagaccagc ttcagctccg
    23821 gctgcgccgc ccaaacccct tcgccaccca aagcctcgaa caccttctgc gccgcatgcg
    23881 gcagcagcgg ggtgagtagc gtgttgcagt ccgaaaccac ctgcagcgcg acatgcagca
    23941 cggtgtcccg acggaccgga tcgtccttca gcttccacgg ctcctgctcg gacaggtact
    24001 tgttggccag cccgaccacc cgcatcgcct cggacgtcgc ctgcttgaac ttgcagcggc
    24061 cccacaggtc gccgaccacg tcgaacgccg cagccgaagc tgaaagcagc tcccgatcag
    24121 cgtcggtcag ctccccagca accggaatcg ccccgacgtt cttgtgcgcc atcgagatgg
    24181 agcggttcac caggttgccc cactcgttcg ccagctcgaa gttgttgcgt cgcacgaact
    24241 cctcccaggt gaagtccgtg tcctggttct ccgggccggc caccgcgatg aagtagcgca
    24301 gcgcgtccgg cccgaactcg gccaggaagt cgcggacgta gatcacgtgc ccgcgggagg
    24361 atgccagctt cgaccccgac atggtcaggt actccgaaga gaccacctct gtgggcaggt
    24421 tcagcggcga gtacgccgac ccgaccccgc ccagatcgcc ctcgccgttg gcgcccagca
    24481 ggatggccgg ccagatcacc gagtggaagg tgatgttgtc cttgcccatg aagtagtacg
    24541 agcgcgcctc agcagactgc caccagagct tccacgcgtc cgggtcgccc gagcgccgtg
    24601 cccactccac tgccgccgac aggtagccga tcactgcgtc gaaccacacg tagatgcgct
    24661 tcatcgacgc gtccgaccac ccctccagcg ggatcggcac gccccaatcg aggtcgcggg
    24721 tgatcgcgcg cggacgcagt tccttgagca ggttgaggct gaacttcatc acgttcggac
    24781 gccagtcggt ccgggtcgcc agccactcgc ccagcgcccc ggcgagcgca ggcagatcaa
    24841 ggaagaagtg ttcggtctcg acgaagttgg gcacctcgcc attgatcttc gacaacgggc
    24901 ggatcaggtc gatcggatcc agctggcgtc cgcagttgtc acactggtcg ccgcgggcac
    24961 cgtcgtatcc gcaaaccggg caggtgccct cgatgtagcg gtcgggcagc gtccgtccgg
    25021 tagacggcga gatcgcgccc tgctgcacct tcgcggtcac gtagccgttc gcgtgcagcg
    25081 cccggaacaa ctcctggacg accgcgcggt ggttcagtgt cgtggtgcgg gtgtacaggt
    25141 cgtaggagca ccccagcgcc tgcaggtcct cggcgatcac ccggtggtac ttgtcggcgg
    25201 tctgctgcgg agtgagtccc tccttatcag cctgcacctg gatggccgtg ccgtgctcgt
    25261 cggtgccgga caccatcagc acctggtttc cggccattcg ctggtaccgg gagaacacgt
    25321 cggacgggac accgaaaccg gagacgtggc caatgtgccg cgggccgttg gcgtagggcc
    25381 aggcgacggc tgccaggatg cgagaactca caatcgctca ttctaggtgt tcgcggctcc
    25441 tcgccagtac gcactcggac gcgtccccgc tccaggccag cagccggctg tgagatcgac
    25501 ctgagttccc ccgccgacac cggccacttt ccgaatctgg tccggttacc cctaccatga
    25561 aggagtgacc ccagagtttc tactcctgtc gcttgtggtt gtaacggcgt tggccttcga
    25621 tttcacaaac gggttccatg acactgcaaa tgccatggcc acctctatcg ccaccaaggc
    25681 gctgaccccg aaggcagcgg tgaccctttc ggcgatcctg aacctcgtcg gcgccttcct
    25741 gtcggtagaa gttgcgatca cggttaccaa cgcggtgatc cggatccaga acccggacgg
    25801 cacccccaag ccggagctga tggcggacgg tggccacggc ctgttgttga tcctgatggc
    25861 aggcctggcc ggcgcaatca tctggaacat cctcacctgg ttgtggggcc tgccctccag
    25921 ttcctcccac gcgctgttcg gcggcctggt cggcgcaacg gtcgcggcca tgggctgggg
    25981 cggcgttaag tgggtcggtg aaggcgcgaa gctcgacggc gtgatcggca aggtggtgct
    26041 gccagccctg ctgtcgccgg ccattgccat catcgtcgcg atgatcggca ccaagctcgt
    26101 cttctgggtc acccgtgggg tggcggagaa gtaccaggaa tcgggcttcc gttggggcca
    26161 gatcggcacc gcgtccctcc tttcgctctc ccacggcacc aatgacgcgc agaagacgat
    26221 gggcgtgatc acgctcgcgc tgatcgcgtc cgggcattgg acggcgaccc atgaagtacc
    26281 gctctgggtg aagatcgcct gcgcggtcgc gatcgcgtcc ggcacctaca tcggtggctg
    26341 gcggatcatc cggacgatgg gcaagggcct ggtcgagatc tcgtcgccgc agggcatggc
    26401 cgcagagagt tcgtccgcag cggtcatcct gacctccagc cagctcggct tcgcactctc
    26461 caccacccac gtctgcaccg gctcgatcct cggctcgggc ctcggccgca agggtgcgaa
    26521 ggtgcgctgg ggcgtcgccg gacggatggg cctggcctgg ctgaccacgc ttccgctggc
    26581 cggcctggtg ggcgcggtga tgtggttcat cgggcacatc atcggcggtt ggctgggctc
    26641 gaccacgatc ttcctcctga tgtgtgcggc cgcgctgtac atgcgctggc actccaagaa
    26701 ggatccgatc ggccaccaca acgtcaacga cgaatgggac gccgagcagc cggacgcgga
    26761 caaggtgccg gtctcgggag gggtgcactg atggatcagc tattcgcagc tctggacgcc
    26821 tcgtggcgcg tcctactggt gggggtggtg ctcggcgccg gcctgcccac catctatgcg
    26881 cttggcatcc gcgcgctggc gtggggcacc ggcggtgagg ccgaggtcca tgaagccggg
    26941 gtggagctca agccacacct gctcggtcgg ctgatcgcct actcgctgtt cgctttcgtg
    27001 gtgctgtgcg tgctgctcgg catcggctac atcgtggccc acggtctggg catgacgatc
    27061 acgttcgacg gcatcgtgcc ggtcttcacg tcaaagtagc cagttccact cgggctcgtc
    27121 ccgccactcg cgccgtagtg cggacatcgt caccgagcct ggccagaaag ctgcgtgccc
    27181 gctgggtgac gccttcggca aacatctcct ccggtatcgg ttgcaccgag atccggacgc
    27241 cgctgcccgc gagcacgagc ggcagcgtgt agccgaccga cgaagggaag ctcgccagtc
    27301 gggcggccac cggggtgcgg cggagttcta cttcaagcgg gacggacgga cgaacaatct
    27361 ccagcccggt ctgagtggcg atccggtcca gtttctcggc ggactcgcgg cggtgggcga
    27421 agtaccgtcc agcgctgccg tcgatggcaa gggcagccac cgcgtccaag taggcacccg
    27481 ggcgcaccac cccggtctcc accaatgacg agccgatcac gtccacgccg ggtcgggtac
    27541 gcacctggcc gaaccggtgc cgcgtccaag cataggaatg gcgctgcact cggctcggcg
    27601 gcagcccggt gactgccatc accgtgaaca actgcagcct ggcggtgtcg aagaaccggc
    27661 gcgcgcgttc ggccagaact gcgcgcaccg gaccgaacga acggccatgc cagcgccgca
    27721 gtcgctcgcc agcggccagc tgggcggcga actccagcgt cgcggtgccg tcatccacca
    27781 cgaccacctg ctgattgccc ggacgcggca gcagcgcttg gatcaacccg gagaacggat
    27841 cgccaagcaa cagtgtgtgc gcgtccgcca cctgggggcg aagttcggcg atcacccgcg
    27901 ccgaagccga gagcgccgtc cgcggatcgt gccattcgac cgcgatgttc tcttccccgg
    27961 cgtaatcggc catggctgcc agctggacgc gggtgcgttc gtccacgggt gccaacacga
    28021 ccagccggat gcgttcggcg gcggcgtgcc ggtggcacca ctccaagaca tgcagcagct
    28081 gagccgggct ctccaccagt gccaggacgg tcacgccacc tccgatccgg actcgctatc
    28141 ggcgaccacc ccgggcactc ggcgcagctt gcgcatcgcc tgtagttcac cgtcgtacac
    28201 ccgcttcacc ccgtcgccga gcgcg
//
LOCUS       DAOPRQ010000032        27956 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_1352_length_27956_cov_11.649009, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000032 DAOPRQ010000000
VERSION     DAOPRQ010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 27956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27956
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1352_length_27956_cov_11.649009"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(230..1114)
                     /locus_tag="PKJ61_05890"
     CDS             complement(230..1114)
                     /locus_tag="PKJ61_05890"
                     /inference="COORDINATES: protein motif:HMM:NF014741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HOC13388.1"
                     /translation="MADTHDSDALGKAATQVLAKVAPADAIEGLETKLQREAEAAHRS
                     RSLRFWRQGGSVRFEGCLPRLVGEQFITLLDAHAEALRRTAVEARDALYVNATPEQRR
                     ADALTHLLQHAATAKPTPGVGTAKVIVKLDYDRLQAGAAGAGVVGDGIQLSAGELRQV
                     CCDAEVIPVVLRGGSEVLGVGRSRRLVTPAIRAALIARDGGCVFPGCTAPPSACEAHH
                     VVPCWLGGSTALSNLVLMCHHHHGLVEPAKYYQRYQWRVRISHADRLPEFAAPARHPQ
                     AGVWLRHARHRHTERIAA"
     gene            complement(1344..2165)
                     /locus_tag="PKJ61_05895"
     CDS             complement(1344..2165)
                     /locus_tag="PKJ61_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13389.1"
                     /translation="MPAAGTPITPTGRVGVVGMALTSASAVPLIALQQFWLGVVALGF
                     GLGLALWVDRVLARTLAVVAVGLLILSATSLEADLTDLGMLRFTLVLGGAVVLPTLLS
                     RWLVAADAVRFPVRGRRWTRTQWWYLAAVIVIAYLLLPFYFVGSGAYANWPAVATANE
                     IGRLFVGVNAVGIWDELFFICIVFTLFRRHVGLRSANVLQAIVFVSFLWELGYRSWGP
                     LLTIPFALVQGWIFATTASLGYVVTVHLLFDAIVFAVLVHAHNPHLFDVFVTIPA"
     gene            2348..3274
                     /locus_tag="PKJ61_05900"
     CDS             2348..3274
                     /locus_tag="PKJ61_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOC13390.1"
                     /translation="MVSVLGSRDEFLRILETGLDADIHVRGNEITFTGTPDAVTVAAD
                     VLTELITIVRTGQGLASDTVERVLGMTANPDVSAAEVLTQNILSSRGKTIRPKTLNQK
                     RYVDAIDAHTVVFGIGPAGTGKTYLAVAKAVQALQAKEVNRIILTRPAIEAGERLGFL
                     PGTLTDKIDPYLRPLYDALHDMIDPDSVPRLLTSGTIEVAPLAFMRGRSLNDAFIILD
                     EAQNTSMEQMKMFLTRLGFGSKIVVTGDVTQIDLPGGVRSGLTAVEEILDGVHDIAFC
                     RLTSHDVVRHRLVGRIVAAYDRFEATKPRKSR"
     gene            3271..3723
                     /gene="ybeY"
                     /locus_tag="PKJ61_05905"
     CDS             3271..3723
                     /gene="ybeY"
                     /locus_tag="PKJ61_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOC13391.1"
                     /translation="MIELNNESGVEVDTERLVQLATFALAALRIHPQAELSILLVDEA
                     TMSAYHEKFMDEPGPTDVLSFPMDEMRAPEADEEPPLGLLGDIVLCPTVTAAQAADSN
                     RTPEEEADYLLIHGLLHLLGHDHAEPAEKKVMFDLNDKIIAAWTASRA"
     gene            complement(3778..4308)
                     /locus_tag="PKJ61_05910"
     CDS             complement(3778..4308)
                     /locus_tag="PKJ61_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOC13392.1"
                     /translation="MIADSVRLALPREAGTIAALQRRSWAASDADLAAQLLGSVDLAA
                     MTASWEAAILRPPLAQFRVLVAIGQERVVGFAALGPSDDPDATEGEDALVAEFVIDPA
                     AMRMGHGSRLLNAAMDTLRADGFSRATWWVRSTDDALREFLISAGWGADGAHTEVAVS
                     ETGPRLRLVRLHTALS"
     gene            4466..6148
                     /locus_tag="PKJ61_05915"
     CDS             4466..6148
                     /locus_tag="PKJ61_05915"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013161320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HOC13393.1"
                     /translation="MRDSLKTPPQLSPDTLRITPLGGLGDVGRNMTSFEINGELLFVD
                     CGVLFPSEDQPGVDLILPGLHLVEDRLDEAVALVLTHGHEDHIGAVPYLLRRRSNLPV
                     LGSKLTLALVREKLREHRIRNVDLRVVSEGDRVRIGEYDLEFMAVNHSIPDALAVVMR
                     TAGGTVLHTGDFKMDQLPLDGRLTDLRGFARLGDEGLDLFMVDSTNAESPGFTTHERD
                     IEPAMERVFAAAKQKLIVACFASHVHRVQQVMDAAVRHGRKVVYVGRSMVRNMGVARD
                     LGYLHVPENTLIDLKQIENFRDDQVVIISTGSQGEPLSALSRISNHEHPVVTAGEGDV
                     VLLASSLIPGNENSVYRVINGLAGNGVTVVHKGNALVHVSGHASAGELLYCYNLLQPR
                     NVLPVHGEVRHLIANAKLAQATGVPAANTIVVEDGAVIDLAKGRARVAGQLDCGYIFV
                     DGLTEGEVGDTELTDRKILGEEGFITLVTVVNLHAKRLVSGPDIHARGFAEDDSVFDD
                     VRPELIAVLEKALADGVEDTFQLQQLVRRTVGRWVSNHYRRRPMILPVVVAT"
     gene            6392..6577
                     /gene="rpmB"
                     /locus_tag="PKJ61_05920"
     CDS             6392..6577
                     /gene="rpmB"
                     /locus_tag="PKJ61_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HOC13394.1"
                     /translation="MAAVCDICAKGPGFGHNVPWSKKKTNRRWNPNIQRVRAVVEGTP
                     KRLNVCTSCLKAGRVTR"
     gene            6690..6980
                     /locus_tag="PKJ61_05925"
     CDS             6690..6980
                     /locus_tag="PKJ61_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13395.1"
                     /translation="MTQPTGTEPADRDPQSGWLWRLRSAAMRLPGLRAVLSAVHAGLG
                     EILIVVLLLIAMPLALLLIVGAFQFGGLPGGFLFLGVVAAAVVGWLVRSRRS"
     gene            7103..9277
                     /locus_tag="PKJ61_05930"
     CDS             7103..9277
                     /locus_tag="PKJ61_05930"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013161318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HOC13396.1"
                     /translation="MFAELAAPLSSVLGERTGKEFAALRVHTLGDLLRHIPRRYLSGT
                     ELTDLATITEGDLVAVIARVADIRRHESRGQPGRNARPGRLEVLLSDGHGRLTATFFG
                     KTHLLTWWEKQLREGVAGVFVGKVGSFRNELQMSHPTFAMMDASGRIVSTSEEKERIA
                     VLSSGGLVGMYPATAKLPTWKVAECARLALSAISGADDPWPEWLRERAGVLGLADAFA
                     AVHQPASLGEAERGADRLRFDEAMATQLTMAYRRADAARQPAQPRPQVTGGLLDAFDD
                     RLPFQLTAGQTEVSAAILADLARDRPMQRLLQGEVGSGKTVVALQAMLTVVDNGGQAA
                     LLAPTEVLAAQHYQTIRSLLGDLGEGRMLGAPDVATDVVLLSGSLPAAAKKSALLRAA
                     SGEAGIVIGTHALLADRVQFADLGLIVVDEQHRFGVEQRAQLLDKASTRPHVLVLTAT
                     PIPRSVAMTIFGDLDVSTLAELPGGRADVATVVVDAASRPQWVERAWSRVLEEVATGR
                     QVFIVAPRIGGQGADGKGVTELADELRAGPLAGVRVGVLHGQLPSDVKDQVMGDFASG
                     QLDVLVATTVIEVGVDVPNASMMVIWDADRFGISQLHQLRGRIGRGSHPGVCLLITSV
                     PAGEPARARLDAIASTRDGFELAELDLEQRREGDVLGSSQAGSRSSLRLLRVLEHADL
                     IALARDLAAECVARDPGLTTPGFADAIRATELLASPDWLERN"
     gene            9549..10133
                     /gene="rsmD"
                     /locus_tag="PKJ61_05935"
     CDS             9549..10133
                     /gene="rsmD"
                     /locus_tag="PKJ61_05935"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HOC13397.1"
                     /translation="MTRIIAGTKKGHRLRTPAHSATRPTSDRVREAAFSLIADWAGTA
                     GEAAENMLTGLGFLDLYAGSGAVAIEAASRGAAPVLAVESDSATARLARGNASDTGLA
                     VTVLAASAERTVAAQATTAYDVIWADPPYALANDRLTAVVEAALSNGWLAEDGLIVLE
                     RSSRDAPPAWPAQVTDSWHRRYGETTLYFATKGR"
     gene            10134..10622
                     /gene="coaD"
                     /locus_tag="PKJ61_05940"
     CDS             10134..10622
                     /gene="coaD"
                     /locus_tag="PKJ61_05940"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015070843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HOC13398.1"
                     /translation="MLAICPGSFDPVTYGHLDVISRAANLFDEVIVAVGQNSAKNYLF
                     TAAERVALVSQTTADLANVRVAALEGLLVDFARAHGAQVLVKGIRFASDFDFELQMAH
                     INGIVGGIETVVLPASAQWSTLSSTMIREVAAYGGDVSAFVPEPVARQIVQKRRKEEA
                     DG"
     gene            10615..11112
                     /locus_tag="PKJ61_05945"
     CDS             10615..11112
                     /locus_tag="PKJ61_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13399.1"
                     /translation="MVERTTEVLRELRELVNNAKAVPMSASCMVNRAQALDLIEKATQ
                     ALSTDVGEAQRVTATSLETLERAQAEAAQIIASAEEKASYLASQTPVMEAAKRKAAAL
                     EAKALADAEGLRREADAYVDTRIATFEASLQKTMGQVRTMRERLASRSSLDDSNTQML
                     PRAKA"
     gene            11206..11766
                     /locus_tag="PKJ61_05950"
     CDS             11206..11766
                     /locus_tag="PKJ61_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002527181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HOC13400.1"
                     /translation="MTPRLPDHRSGLVLDTHELTRRAGVLREVTRVTEAPVDLGIEMI
                     GVPPGSPVDLDLRLESVVEGVLVSGTATVRLRGTCARCLDDIDAQVEVDLQELYCYPG
                     KELDDAEALHIEGELIDLEPVLRDAVVLELPFTPLCQPDCAGLCPECGANLNRDPQHS
                     HAEPIDPRWAELEAWPAPSNESKNKE"
     gene            11771..11962
                     /gene="rpmF"
                     /locus_tag="PKJ61_05955"
     CDS             11771..11962
                     /gene="rpmF"
                     /locus_tag="PKJ61_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002514262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HOC13401.1"
                     /translation="MAVPKRRMSRSNTRARRSQWKTSAPTLVTCANAACGAKHLPHTA
                     CPECGQYGPRAARRQILES"
     gene            12015..12719
                     /gene="rnc"
                     /locus_tag="PKJ61_05960"
     CDS             12015..12719
                     /gene="rnc"
                     /locus_tag="PKJ61_05960"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HOC13402.1"
                     /translation="MIEELGIQLDPQLFRLAVTHRSYAYENGGLPHNERLEFLGDAVL
                     GIVVTEFLYRSFPDLPEGRLAKLRAAVVNAHSLAGVARSLDLGSEILLGKGEVATGGS
                     DKSSILADSLEAVLGAVLISSGRDAADRLVHHLFDPLVLDAAVLGAGLDWKTSLQELS
                     AELELGVPVYRVTESGPDHEKRFEAAAVIGARAFGTGSGTSKKQAEQGAAKSAFAALS
                     DVAAARSESALSGDSA"
     gene            12719..13576
                     /gene="mutM"
                     /locus_tag="PKJ61_05965"
     CDS             12719..13576
                     /gene="mutM"
                     /locus_tag="PKJ61_05965"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017934049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HOC13403.1"
                     /translation="MPELPEVEVVRSGLAQLIPGRRISAVTVLHPRPVRRHVGGPDDF
                     ASVLVGRRFAEPKRRGKFIWLPFTDGDALMAHLGMSGQFRVDAPEAELPPQCRVRFAF
                     DDGGPEIRFADQRMFGGLWLSPGGAELPVEVAHIARDLFDPDLDRDALVADIRRRHSG
                     IKRVLLNQTVVSGIGNIYADEALWRARVHYDTPADRLSPALVVELLDAAVAVMRESLA
                     QGGTSFDALYVNVNGSSGYFGRSLSAYGREDLPCLRCGRAIVREPFMNRSSYRCPACQ
                     RRPRTQVVR"
     gene            13644..14201
                     /locus_tag="PKJ61_05970"
     CDS             13644..14201
                     /locus_tag="PKJ61_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002527185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HOC13404.1"
                     /translation="MFADVTGAEPTQRFQRVLRQFVKFGIVGGAGVVVNMLVAIAMNK
                     ANGGTINAQRILFAIPGTNFNFRYTVLVWLVAFLVANVFNFQLNRSWTFRHTRKAPWW
                     QEFWPFLAVGSAAALLGIFIKVAFTNPTSWFYLPEPWFHENAGFASREYWAQLFTIVI
                     TMPINFVANKLWTFTHVRNKHQAAV"
     gene            14205..17765
                     /gene="smc"
                     /locus_tag="PKJ61_05975"
     CDS             14205..17765
                     /gene="smc"
                     /locus_tag="PKJ61_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HOC13405.1"
                     /translation="MYLKSLTLRGFKSFASTTNLVFEPGITCVVGPNGSGKSNVVDAL
                     AWVMGEQGAKSLRGGKMEDVIFAGTSGRAPLGRAEVVLTIDNSDGSLPIDYTEVTISR
                     TMFRTGGSEYAINGSPARLLDVQDLLSDSGIGREMHVIVGQGQLDSILQATPETRRGF
                     IEEAAGVLKHRKRKEKAQRKLEATAANLNRLSDLLGEIRRQLKPLGRQAEVARKAAVI
                     QADLRDARARLLADDLVAATSSLATDLAAEAELKQRRLRIEAELTSAREAEQAAEVAA
                     RDALPALTAAQETWYALAGLAERVASTTTIASERLRNAAAIGTDERPGRDPEAIEREA
                     AEIRKTEASVQAEVAERTAALAAATDARNAAETAHAEAEKQYSAQLRAIADRREGLAR
                     LAGEVNTLRSRSEAAGAEIERLVAAKAEAEDRATNAKRQFAQLETRLAGLSAGESGLD
                     DAYEAAEADVAARLTALEELQTAERAATAERGAAAARAEALALGLERRDGTAALLAAG
                     DQLDGLLGSVAALISVPVGKEVAIAAALGSAADAIAVTGVDAAVRAFDHLKAEDLGRA
                     GMLLGADLPADTSGWPKLPANACYAAGLVDCAASLRPAIERLLFKVAVVDDLAQGREL
                     VAKLPDVTAVTTGGDLLSSWFAAGGSSAKPSLLEVQAALDETNEKLSAAQAEVERTRF
                     AIAEAEQLLASARERSDAALARLHESDAEHAAMAEEAGELNQIVRSAQAEAGRLAQAA
                     DNAATARDHALANLSELETRLELASEQTETGEADPTERDHLGDLARAARAAEMEARLA
                     LRTVEERARAFAGRADSLLRAADAERSARAKAAARRELLRREAEVAAGVLKAAEWLAE
                     QVSASRALAETERMRAQEARSVAETELTAVRQRGRELAKAFENLVDTVHADELARAQQ
                     QMKIDALAEKALAELGLEPEALVGEYGPEQLVPVLVNADGKPLTEDDPQPDPVPYVRA
                     EQERRLRAAERDLAVLGKVNPLALEEFDALSERHTFLAEQLADLRKTRDDLNGIIADV
                     DTRVQEVFAAAYADVEQAFEDAFARLFPGGEGRLVLTEPGEWLTTGVDVEARPAGKKV
                     KRLSLLSGGERSLVAVAFLVALFKARPSPFYILDEVEAALDDTNLGRLLEIYEELRAN
                     SQLLVITHQKRTMEIADALYGVTMRSDGVSAVISQRLRDA"
     gene            17901..18146
                     /locus_tag="PKJ61_05980"
     CDS             17901..18146
                     /locus_tag="PKJ61_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13406.1"
                     /translation="MSYWIITVALVAIALCVIAVVAVGMNGSLASKAPEFANVMANTA
                     KHLNGDADPPQGLVEFFEEIEDIRPKSADSAAEAPKR"
     gene            18181..19338
                     /gene="ftsY"
                     /locus_tag="PKJ61_05985"
     CDS             18181..19338
                     /gene="ftsY"
                     /locus_tag="PKJ61_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOC13407.1"
                     /translation="MSGVENYFWYVLAAVAAVLIVAAVTIVVGNRKGLPPAEPTPELP
                     SAEREEFGEPVEPAPAEVIPAAPAEPSAPVLETPEPPASRMSRLRRKLSASNNPFARG
                     LLGLLAVDKLDADAWDDLEATLITSDLGAGPTDELITALRRELSLDAVSDPDKAKQVL
                     RTELLKLVATTADRSLNVTGADGLPGVVLVVGVNGTGKTTTIGKLSRVLVAEGHSVVL
                     AAADTFRAAAADQLQTWGSRVGVPVVRGPEGGDPASVAFDAVDQGIASGADVVVVDTA
                     GRLHTKTGLMDELGKIKRVIERKAPVTEVLLVIDATTGQNGLAQARVFAEVVDVTGIV
                     LSKLDGSAKGGIVVPVQRELGVPVKLIGLGEGADDLAPFEAEGFVDALLAQ"
     gene            19335..19826
                     /locus_tag="PKJ61_05990"
     CDS             19335..19826
                     /locus_tag="PKJ61_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HOC13408.1"
                     /translation="MTTIRPARPEDLPALLEIYNDSVVRDTASWDLLPWSPVQHAEWY
                     ATKVEHGWPVLVAEVDGEVAGYATYGQFRPKAGFTLSMEHSVYLRPAFQGRGLGRALM
                     TAIIAAARENGVHVLIGGLSADNDASFALHRSLGFVEVGRLPEVGRKFDRWLDLVFVQ
                     LTL"
     gene            19997..21565
                     /gene="ffh"
                     /locus_tag="PKJ61_05995"
     CDS             19997..21565
                     /gene="ffh"
                     /locus_tag="PKJ61_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016665670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HOC13409.1"
                     /translation="MFDTLSDRLSAAFRNLRGKTRLSDADIDATAREIRLALLEADVN
                     LGVVKDFIAAVKERAHGAEIHAALNPAQQIVKIVNEELIAILGGEARPLRFAKRPPTV
                     IMLAGLQGSGKTTLAGKLGKWLKDQGHSPLLVASDLQRPNAVNQLQVVGERAGVHVFA
                     PEPGNGIGDPVGVARTSILHAMSKLYDVVIVDTAGRLGVDAELMQQAADIRDAVNPTE
                     ILFVVDAMIGQDAVNTARAFADGVGFDGVVLSKLDGDARGGAALSIARVTGKPIMFAS
                     NGEGLGDFDAFHPDRMASRILGMGDLLSLIEQAEKTFDAEESAKAAQKLLQGKGGEFG
                     LDDFLSQMQAVRRMGPLSKILGMMPGMGQMKDQINNIDEREIDRLEAIIYSMTPAERA
                     NPKLLDGSRRARIATGSGTKVSDVNSLVNRFFEARKMMQSLGGAMTGMGGRRPAAKQQ
                     PKKKKGNHKVSGNPAKRTGGGAAALPPAASAPTSAFGLPTQPTEADLAKAVGDFQLPA
                     ELQQLFNPKNAGPR"
     gene            21883..23052
                     /locus_tag="PKJ61_06000"
     CDS             21883..23052
                     /locus_tag="PKJ61_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOC13410.1"
                     /translation="MSHHHAPPGLGEPEFFAHDHGGRESPSTRPGYPLHLQGVLLPEG
                     EPRDLWVADGLIRTEPVPDAVTVCQGAWILPGLVDAHCHIGLGANGAVPDEVAEAQAL
                     TDRDAGTLLIRDAGSPIDTHWIDEREDLPRIIRAGRHIARPKRYIRNYAAEVEPEELI
                     AEVARQAVRGDGWVKLVGDWIDRDRGDLAPLWPAEVAAAAIERAHQLGARVTAHCFGE
                     ESVQQLVRAGIDGIEHGTGLDAATIELMAERRVALVPTLVNLENFPAFADAGEAKFPA
                     YAAHMRTLHARRFETIGAAIEAGVPVFAGTDAGGSISHGLVGTEIGMLAKLGGAEFAL
                     GAASWRAREWLGAPPVVDGAWADLVVYAADPRLDPGVTRHPSLVILRGRVVASAH"
     gene            complement(23211..24476)
                     /locus_tag="PKJ61_06005"
     CDS             complement(23211..24476)
                     /locus_tag="PKJ61_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009080446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HOC13411.1"
                     /translation="MFRPDSADRPRDKARLGLLGMLGPAFVASVAYVDPGNVAANLSA
                     GADYGYALVWVLAVASLMAMVIQYQSAKLGLVTGATLPQAMADRFAGHRYQRPLTVGY
                     GLQAFVIAIATDLAEVVGGALGLYLLFGLPLWAGSIVVGVVAMVLLWVLRSRGEGVFE
                     AGVGALLAVVALGFVGALFFKAPSWDATLAGLWPSVPDRDAWPLIAAMLGATVMPHAI
                     YLHSALAIDRHRPDGHLDRPLRRLLRAQKLDVGLALVLSGSVNISMLLLGAVALAGVV
                     GDPIEAIHVTLGTDLGPWPAAVFAIGLLASGIGSAVVGTHAGARILKDMHVWQVEPTL
                     RRAITIGPAVLLLLTGVSPTSVLIWSQIVLSFGVALAAAPLALVTGDRRLMGEFVDRA
                     WQRALNWLIVTLIIGLNLVMIWWALSRGS"
     gene            24683..25111
                     /gene="rpsP"
                     /locus_tag="PKJ61_06010"
     CDS             24683..25111
                     /gene="rpsP"
                     /locus_tag="PKJ61_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016455593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOC13412.1"
                     /translation="MAVKIRLKRLGKIRSPHYRIVVMDARAKRDGRAIEEIGLYHPKN
                     DPSVIRIDSERAQYWLGVGAQPTEAVVALLKRTGDWQKFSGDTSPSGVTPQPEPKDKV
                     ALFNAALAEADSEPATAAISKSKKKAAEADAEAPAEAEEA"
     gene            25114..25368
                     /locus_tag="PKJ61_06015"
     CDS             25114..25368
                     /locus_tag="PKJ61_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HOC13413.1"
                     /translation="MLAEALEHLVRGLVPNPDDVRVRESDRGRTRTLEVRVHPEDIGK
                     VIGRQGRTAGALRTVIAALAGREQVRVDFVDVDRRPRRRS"
     gene            25458..25976
                     /gene="rimM"
                     /locus_tag="PKJ61_06020"
     CDS             25458..25976
                     /gene="rimM"
                     /locus_tag="PKJ61_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862541.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HOC13414.1"
                     /translation="MTDHLIVGTVGRAHGLRGQVTVRPRTDSIDERFASGAKVSAGDR
                     ELTVTGHGLQGGRLVVSFAEVRDRTAADALRGVDLWVQGTPTDIDDDEFHDSTLVGLR
                     ACDPAGAELGEVVAVLHNPAQDLLVIRTPLGERLVPFVTELVPEVDPAAGQVVINPIP
                     GLLSDVEVRDAD"
     gene            25966..26670
                     /gene="trmD"
                     /locus_tag="PKJ61_06025"
     CDS             25966..26670
                     /gene="trmD"
                     /locus_tag="PKJ61_06025"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016455596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOC13415.1"
                     /translation="MRIDLVSIFCDYFAPLQLSLVGKAISSGIVTLGVHDLRDWTHDR
                     HRTVDDTPYGGGAGMVMKPEPWGEALDELVPPDGPKPLLVVPTPSGVPFTQALAHELA
                     GRDWLLFACGRYEGIDARVVAHATSRMEVLEVSLGDYVLNGGEVAALAITEAVVRLLP
                     GVLGNPESLVEESHGDDLDGLLEYPVYTKPANWRGLEVPAVLLSGHHGQVSEWRLAQS
                     RERTAQRRPDLLEGSN"
     gene            26800..27528
                     /locus_tag="PKJ61_06030"
     CDS             26800..27528
                     /locus_tag="PKJ61_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002518436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HOC13416.1"
                     /translation="MATTTLTPHQRALRSTVTLKVIMAVTGLLLIAFLLMHAFGNSKM
                     FLGEDSFNHYAEWLKGQTEDGGILYPLLPAGWFIWIFRVVLLAAVVLHIYSAVTLWNR
                     ALAANPSTYAAKRDLARTYSARTMRWGGIILAGLLIFHLAQFTVHAALTGGEYGADYA
                     SSPYKMVIGEFSQWWMVLLYAVWMVAVCMHIRHGFWSAFATLGANTSAKARAVLNGCA
                     WAVALILYIGFMIMPVAVLFGWIN"
     gene            27528..>27956
                     /locus_tag="PKJ61_06035"
     CDS             27528..>27956
                     /locus_tag="PKJ61_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017934659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOC13417.1"
                     /translation="MTTTTKAPAAALPGADYFTLGANIADTKAPAGPIEGKWAKRKFE
                     AKLVNPSNRRKLTVIIVGTGLAGGAAAASLGEAGYNVLNFCYQDSPRRAHSIAAQGGI
                     NAAKNYRNDNDSTFRLFYDTVKGGDYRARETNVYRLAEVSA"
BASE COUNT         4637 a         9446 c         9565 g         4308 t
ORIGIN      
        1 cagctatgga gccggcgatc gccaccgagt tggtggctgc aaccgatcgt ttcgcacgct
       61 agttgtcaca cgcgcccgcc aaaggctttg ctgcgggtgt tcttccggcc gagccactgc
      121 tatcactgag cttgacgaag gtctgggcga ggcttcggca ggcttggccg gcgtcgcttg
      181 ggtgttgtgg gcaggctccg tggccggccc acactgcggg ccagtcgggt caagccgcta
      241 ttcgctcggt gtgccggtgg cgggcgtgtc gcaaccacac tccggcctgc ggatgccggg
      301 caggtgcggc gaactccggt agccgatcag cgtggctgat ccggactcgc cattggtagc
      361 gctggtagta cttggctggt tcgaccaggc cgtggtggtg gtgacacatc aggaccagat
      421 tcgaaagggc ggtcgaaccg cccagccagc agggcaccac gtgatgggcc tcgcacgctg
      481 atggtggtgc ggtgcagcct gggaacacgc acccaccgtc gcgggcgatc agcgcggccc
      541 gtatcgctgg ggtcaccagc cggcgggagc ggcccacgcc caacacctcg ctaccaccac
      601 gaaggacgac cgggatgacc tcagcgtcgc aacacacctg gcgcagttca cctgcggaca
      661 gttggatgcc gtccccgacc acaccggcac cagcagcacc ggcttggagt cggtcgtagt
      721 cgagcttcac aatcaccttc gcggtaccca caccaggtgt gggcttagcg gtcgcggcat
      781 gctgcagcag gtgggttagc gcgtcggctc ggcgctgctc tggggtggcg ttcacgtaca
      841 gcgcgtcccg ggcctccacc gccgtccgcc gcagcgcctc ggcgtgcgca tccagcaggg
      901 tgatgaactg ctcacccacc aacctcggca ggcagccctc gaaccgcacc gaaccaccct
      961 gccgccagaa ccgcaacgaa cgggaccggt gcgcggcctc ggcctcacgc tgcaacttgg
     1021 tttcgagtcc ctctatggcg tctgccgggg cgaccttcgc caacacctgc gttgcggcct
     1081 tgcccaacgc gtccgaatca tgcgtgtcag ccatgctcca gcaactgctc ggcctgcgcc
     1141 tgctgcccag aatccagctg tggggccagc ccagcgagca cggtgttgat cgcccgcacc
     1201 tgacccgtac tcacctttcc agcgaccgcg gcctgaccga gtacctgaca cttttcgtcc
     1261 agaacagctc caagcagaca tcatccccag attctcagca aaccctcaga aatcccattc
     1321 aatggtcagc gcggacagcc tgctcaggcg gggatcgtca cgaacacgtc gaacaggtgc
     1381 gggttgtgcg cgtgcaccag cacggcgaag acgatcgcgt cgaagagcag gtgcacggtc
     1441 accacatagc ccagcgaagc ggtggtggcg aagatccagc cctgcaccag ggcgaacgga
     1501 atcgtcagca gcggtcccca ggatcggtag cccagttccc acaggaacga cacgaacacg
     1561 atcgcctgca gcacgttggc cgaccgcagt ccgacatggc gccggaagag cgtgaacacg
     1621 atgcagatga agaagagttc gtcccagatg cctaccgcgt tcacaccgac gaacagccgg
     1681 ccgatctcgt tcgccgtcgc caccgccggc caattcgcgt atgcgcccga gccgacgaag
     1741 tagaacggca gcagcagata ggcgatcacg atcaccgccg ccaggtacca ccactgggtg
     1801 cgggtccacc gccgtccgcg gacggggaag cgcaccgcgt ccgcagccac caaccaccgc
     1861 gacaacagcg tgggcaggac gactgcaccc ccgagcacca gggtgaaccg caacatcccg
     1921 agatcggtca agtcagcctc cagcgaagtg gccgacagga tcagcaaccc gactgcgacc
     1981 accgcgaggg tgcgggccag gacgcggtcg acccaaagcg cgaggccaag tccgaagccg
     2041 agggcgacca cgcccagcca gaactgctgc agcgctatga gcgggacagc gctcgcgctg
     2101 gtcagcgcca tgccaacgac accgacccgt ccggtcgggg tgatcggcgt gcccgcagca
     2161 ggcatcgacg cctccctgga aatggttgtc cgacattgtc tccgctcgga cgcacccgcg
     2221 tcaccgccag gggagtgatg tcagtgctgc tgggtacgat gaacccgata cctgaggagg
     2281 aaccctgaac caaccgagta ccgcggcaat cgctgaacgc cgggtcgcta ttcccgcttc
     2341 catcccgatg gtgtcggtgc tgggttcgcg cgacgagttc ctgcgcatcc tggagaccgg
     2401 gctggacgcc gacatccacg tccgcggcaa cgagatcacc tttaccggca caccggacgc
     2461 ggtgaccgtc gcggccgatg tgctgaccga gctgatcacc atcgtccgca ccggccaggg
     2521 actcgcgtca gacacggtgg aacgggtgct gggcatgacc gccaacccgg acgtgagcgc
     2581 ggccgaagtg ctcacccaga acatcctgtc cagccgcggc aaaacgatcc ggccgaagac
     2641 gctgaaccag aagcgctacg tcgacgcgat cgacgcgcac accgtggtgt tcggcatcgg
     2701 cccggccgga accggcaaga cgtacctggc cgtcgccaag gctgtccagg cgctgcaggc
     2761 taaggaagtg aaccggatca tcctcacccg gccggcgatc gaggcgggcg agcggctcgg
     2821 tttcctgccg ggcaccctga ccgacaagat cgacccgtac ctgcgtccgc tctatgacgc
     2881 gttgcacgac atgatcgacc ccgactcggt gccgaggttg ttgacctcgg gcacgatcga
     2941 ggtggcgccg ctggccttca tgcgcggccg cagcctgaac gacgcgttca tcatcctcga
     3001 tgaggcccag aacacttcga tggagcagat gaagatgttc ctgacccggc tcggcttcgg
     3061 gtcgaagatc gtggtcaccg gtgacgtcac ccagatcgac ctgccgggcg gcgtccgcag
     3121 tggcctgacc gcggtcgagg agattctgga cggtgtgcat gacatcgcgt tctgccggct
     3181 gaccagccat gatgtcgtcc gccaccgcct ggtcggccgg atcgttgctg cctacgaccg
     3241 cttcgaagcg accaaaccca ggaagagccg atgatcgaac tgaacaacga gtccggcgtt
     3301 gaagtcgaca ccgagaggct cgtgcaactg gcgacgttcg cgttggccgc gctgcgcatc
     3361 catccccagg ccgaactgtc gatcctgctg gtggatgaag ccacaatgag cgcgtaccac
     3421 gagaagttca tggacgagcc gggcccgacc gacgtgctca gcttcccgat ggacgaaatg
     3481 cgcgcaccag aggcggacga ggaaccacca ctcgggttgc tgggcgacat cgtgctctgc
     3541 ccaaccgtga ccgcggccca ggcggccgac agcaaccgca cgcccgagga ggaggccgac
     3601 tacctgctga tccacggctt gttgcatctg ctcggccacg accacgccga gccggcggag
     3661 aagaaggtca tgttcgacct gaacgacaag atcatcgccg cttggacggc ttcgcgagcc
     3721 tgaggcaaga gggcgcggtc ggtctcgacg gtcctggacg ttgggacgtc cgggtgttca
     3781 gctcagcgcg gtgtgcaggc gcactagccg cagccgagga ccggtctcgc tgaccgccac
     3841 ctcggtgtgg gcaccgtcag cgccccagcc ggccgagatc aggaactcac gcagcgcgtc
     3901 gtcggtcgag cgcacccacc aggtggcacg gctgaagccg tccgcgcgca gggtgtccat
     3961 ggccgcgttc agcaggcgcg atccgtgtcc catacgcatc gctgctgggt cgatcacgaa
     4021 ctccgccacg agcgcatcct cgccctcggt agcgtcggga tcgtcggacg ggccgagcgc
     4081 tgcgaagccc accacccgct cctggccgat cgccaccagc acgcggaact gggccagggg
     4141 cggacgcagg atcgcggcct cccaactggc cgtcatggcg gcgaggtcca ccgaaccaag
     4201 tagctgggct gccaggtccg cgtccgatgc cgcccacgac cggcgttgca gcgcagcgat
     4261 ggtgcccgct tcacgtggaa gggcaaggcg gacggagtcg gcgatcacgg cgtcactgta
     4321 cccaccccac cgcgcggtac cgactcgcca tcgggtcgca tgcggcgccg ccgtcgcagt
     4381 gtggtcgaac agactgaggc aactgcgcag gctggcaggc cgtgccctca ggctgcgccg
     4441 ccgcaagaac cgataggttg gggccgtgcg agatagcttg aaaaccccac cccagctgtc
     4501 gccggatacg ctgcggatca ccccgctcgg cggcctcggc gatgtcggcc gcaacatgac
     4561 ttcgttcgag atcaacggcg agttgctgtt cgtcgactgc ggtgtgctct tcccgtccga
     4621 ggaccagccg ggtgtcgacc tcatcctgcc cgggttgcac ctggttgagg accgcctcga
     4681 cgaggccgtc gcgctggtgc tgacccacgg gcacgaggac cacatcggcg ccgtcccata
     4741 cctgctgcga cgacgcagca acctgcccgt cctgggttcg aagctgacgc tcgcgctcgt
     4801 gcgggagaag ctgcgcgagc accgcatccg caatgtcgac ctgcgggtgg tgagcgaggg
     4861 tgaccgcgtc cggatcggcg agtacgacct ggagttcatg gcggtcaacc actccatccc
     4921 ggacgcgctc gcggtggtca tgcggaccgc tggcggcact gtgctgcaca ccggcgactt
     4981 caagatggac cagttgccac tcgacggccg actcaccgac ctgcggggct tcgcccggct
     5041 cggtgacgag gggctcgacc tgttcatggt tgactccacc aacgccgagt cgccgggctt
     5101 caccacgcac gagcgggaca tcgagccggc catggagcgg gtgttcgcgg ccgccaagca
     5161 gaagctgatc gtggcctgtt tcgcttccca tgtgcaccgg gtgcagcagg tgatggacgc
     5221 ggccgtccgg catggacgca aggtggtcta cgtcggccgt tcgatggtcc gcaacatggg
     5281 ggtcgcgcgt gacctcggct acctgcacgt gcccgaaaac acgctgatcg acctcaaaca
     5341 gatcgagaat ttccgcgatg accaggtggt gatcatctcc accggctccc agggcgagcc
     5401 gctctcggcg ctgtcgcgga tctcgaacca tgaacatccc gtggtgaccg cgggggaggg
     5461 cgacgtggtg ctgctcgcgt cctcgctgat cccgggcaac gagaattcgg tctaccgggt
     5521 gatcaacggg ctggccggta acggcgtcac ggtcgtccac aagggcaacg ccctggtgca
     5581 cgtctccggc cacgcctccg cgggcgagtt gctgtactgc tacaacctgc tgcagcccag
     5641 gaacgtgctg ccggttcacg gtgaggtgcg gcacctgatc gccaacgcaa agctcgccca
     5701 ggcgaccggc gtgccggcgg cgaacacgat cgtggtcgag gacggcgcgg tgatcgatct
     5761 ggccaagggc cgggcgcgcg tggccggcca gctggactgc ggctacatct tcgtggacgg
     5821 gctcaccgaa ggcgaggtgg gcgacaccga actgaccgat cgaaagatcc tcggcgagga
     5881 gggcttcatc acgctggtca cggtggtcaa cctgcatgcc aagcggttgg tcagcggtcc
     5941 cgacatccat gcgcgcggct tcgctgagga cgactcggtc ttcgacgacg tgcgtccaga
     6001 actgatcgcc gtgctggaga aggcgttggc ggacggggtc gaggacacct tccagctgca
     6061 gcagttggtg cgccggacgg tcggacgctg ggtcagcaac cactaccgcc gccggccgat
     6121 gatcctgccg gtggtggtgg ccacgtaagc cgctgccgcc cagccgaacc aggctcaaac
     6181 ccaatttcgc tcccgaatgg gactcccgct cccgaactat cgggagcgag ggtcccattc
     6241 gggagcgaag ctgcgttccc cgaccggcac cccgtccgcc gattggcggg tgcgggcggg
     6301 gttcaggtat cgtagctagg tccctgtcgt ggggaccgac ttccgggtgc gaatcgtgcc
     6361 cgcacttgat tagacaacga ggagtactcc ggtggctgcc gtctgtgaca tctgcgccaa
     6421 gggtcccggc ttcgggcaca acgtcccctg gtcgaagaag aagaccaatc ggcgctggaa
     6481 cccgaacatt cagcgggtcc gcgctgtggt tgaaggcacc cccaagcgcc tcaacgtgtg
     6541 cacctcctgc ctgaaggccg gtcgcgtcac ccgctgaaac cctgacgaga gacccgctgc
     6601 ggcgggtctc ttcgcatgtc cggagccgtc gttgcggcgg acggctcgga cttcacccgt
     6661 actcaaccgc tcgtgggacg atctgacctg tgacccagcc aaccggcacc gaacccgctg
     6721 accgtgaccc gcagtcgggc tggctgtggc gcctgcgctc agcggcgatg cgactgcccg
     6781 gcctgcgtgc tgtgctttca gccgtccacg ccgggctggg cgagatcctg atcgttgtgc
     6841 tgctgctgat cgccatgccg ctggcgctgc tgttgattgt cggcgccttc cagttcggcg
     6901 gcctgcctgg cggcttcctg ttcctgggcg tggtcgcggc cgcggtggtc gggtggctcg
     6961 ttcgaagccg caggtcgtag gcggtcggcc gccgagctgc tcgatcacgc ggggcttccc
     7021 ggctctgccc gtgcgcgact gtcggccgga ccgcctaggc tgaccaatcg tggagatcgg
     7081 gctgagtggt tggcggacgc ggatgttcgc cgaactggcc gcgccgttga gttcggtcct
     7141 gggggagcgc accggcaagg agttcgcggc gctgcgcgtc cacaccctcg gcgacctgct
     7201 ccggcacatc cccaggcgct acctctccgg cactgaactc accgatttgg ccacgatcac
     7261 tgagggcgat ctggtcgcgg tgatcgctcg ggtcgcggac atccgccgtc acgagagccg
     7321 cggccaaccc ggccgcaacg cccgtccggg acgacttgag gtgctgctca gcgacggcca
     7381 tggccggctg acggcgacct tcttcggcaa gacccacctg ctgacctggt gggagaagca
     7441 gcttcgcgaa ggcgtggccg gagtcttcgt cggcaaggta ggcagcttcc gcaacgagtt
     7501 gcagatgagc cacccgacct tcgcgatgat ggacgcgagc ggacggatcg tctccacttc
     7561 ggaggagaag gaacgcatcg ccgttctttc cagcggcggc ctggtgggca tgtacccggc
     7621 gacggccaag ctccccacct ggaaagtggc cgaatgcgcg cggctggcgc tgtccgcgat
     7681 ctccggtgct gacgacccgt ggcccgagtg gttgcgggag cgtgcgggcg tgctgggctt
     7741 ggcggacgcg ttcgcggccg tccaccagcc ggccagcctc ggcgaggccg agcgtggcgc
     7801 cgatcggctg cgttttgacg aagccatggc cacccagctg accatggcct accggcgagc
     7861 ggacgcggcc aggcagccgg cgcagccccg tccccaggtc accggcggac tgctggacgc
     7921 cttcgatgac cggctgccgt tccagctcac cgcgggccag accgaggtca gcgccgccat
     7981 cctcgccgac ctggctcgtg atcggccgat gcagcggctg ctgcagggcg aggtgggttc
     8041 cggcaagacc gtggtggcgc tgcaggcgat gctgacggtg gtcgacaacg gcggtcaggc
     8101 cgcgctgctg gcgccgaccg aggtgctggc agcacagcac taccagacca ttcgttccct
     8161 gctgggcgac ctcggcgagg gccgcatgct cggggcgccg gatgtcgcta ccgacgtggt
     8221 gctgctgtcc ggatcgctgc cggcggccgc gaagaagtca gcgctgctgc gcgcggcgag
     8281 tggggaagcg ggcatcgtca tcggcacgca cgcgctgctg gccgaccggg tgcagttcgc
     8341 cgatctggga ttgatcgtgg tcgacgagca gcatcgtttt ggggtggaac agcgggctca
     8401 gctactcgac aaggcgagca cccgtccgca tgtgctggta ctcaccgcaa ccccgatccc
     8461 gcgctcggtg gcgatgacga tcttcggcga cctggacgtc tccacgctgg ccgagctgcc
     8521 cggcgggcgc gccgacgtgg ccacggtcgt ggtggacgca gccagccgcc cgcagtgggt
     8581 ggagcgcgcc tggagccgag ttctggagga ggtcgcgacc ggccggcagg tcttcatcgt
     8641 tgcgccgcgg atcggcggcc agggcgccga cggcaagggc gtcaccgagc tcgctgacga
     8701 gctgcgcgcc ggcccgctgg ccggggtccg ggtcggcgtg ctgcatgggc agttgccgag
     8761 cgacgtcaag gaccaggtga tgggcgactt cgcgtccggg cagctcgacg tgctggttgc
     8821 gaccaccgtg atcgaggtcg gtgtggacgt gccgaacgcc tccatgatgg tgatctggga
     8881 cgccgaccgg ttcggcatct cgcagttgca ccagcttcgc ggccggatcg gccggggcag
     8941 ccaccccggc gtgtgcctgc tgatcaccag cgtgcccgcg ggcgaaccag ctcgcgcgcg
     9001 actggacgcc atcgcgtcca cgcgggacgg gttcgaactc gccgaactcg acctggagca
     9061 gcgccgtgaa ggggacgtgc tgggcagctc gcaggcgggc agccgatcta gcctgcgcct
     9121 acttcgggtg ctcgagcacg ccgacctgat cgccctcgcc cgcgacctgg ccgccgaatg
     9181 tgtggcccgc gaccccggtt tgaccacccc aggcttcgcg gacgcgatcc gcgccaccga
     9241 actgctcgct tcccccgact ggctcgaacg caactagggc agtgctgaac gacgtgcccc
     9301 gagtcggatt ccaccgatcg cgctgagaga tcccggcctg cgccgggatg acagtaaaaa
     9361 gcgggccggg atgacagtgg aaagcgggcc gggatgacag tgggaaatgg gccgggatca
     9421 cagtggggag tggcctgggc cggcgaacga tccgtgagct tgacgaaggg tcgatcgggg
     9481 cttgcgaacc ttgtcgcgga acggcgccga aaccggcgca acccgtcggc accgttaacc
     9541 tgaccaccgt gaccaggatc atcgcgggaa cgaagaaggg ccaccggctg cggacgccgg
     9601 cgcactcggc cacgcgcccg accagcgacc gggtgcgcga ggccgcgttc tctctgatcg
     9661 cggattgggc cggaaccgcg ggcgaggccg ccgagaacat gctgaccggg ctgggcttcc
     9721 tcgacctcta cgcgggctcg ggcgcggtcg cgatcgaggc ggcgagccgt ggggcggccc
     9781 ccgtcctcgc ggtcgagagc gattcggcca ccgcgaggct ggccagggga aacgccagcg
     9841 acaccggtct cgcggtcacc gttctggcgg ccagcgctga gcgcacggtg gcggcccagg
     9901 ccactaccgc ctacgacgtg atctgggccg acccgcccta cgccctcgcc aacgatcggc
     9961 tcaccgcagt cgtcgaagcc gccctgagca acggttggct ggctgaagac gggctcatcg
    10021 ttctagagcg ctcatcgagg gatgcgccgc cagcgtggcc cgcacaggtg accgatagct
    10081 ggcaccggcg ttacggtgag accacgttgt acttcgccac gaaggggcgc tgaatgcttg
    10141 cgatctgccc ggggtcgttc gaccccgtca cctacgggca cctcgacgtg atctcgcgcg
    10201 ccgccaacct cttcgacgag gtgatcgtcg ccgtcggcca gaactcggcc aagaactacc
    10261 tgttcaccgc ggctgagcgc gtcgccctgg tcagccagac cacggccgac ctggccaacg
    10321 tccgcgtggc ggctctcgag gggctgctgg tcgatttcgc cagggcccat ggtgcgcagg
    10381 tgctggtaaa aggcatccgg ttcgcctccg atttcgattt tgaactgcag atggcgcata
    10441 tcaacggcat cgtcggtggt atcgagacag tcgtattgcc cgcgtcggcc caatggtcca
    10501 cgctttcctc gacgatgatc cgcgaagtcg cggcatacgg cggcgacgtg tcggccttcg
    10561 tgcccgaacc ggtcgcgcgg cagatcgtcc agaagcggag aaaggaagaa gctgatggtt
    10621 gagcggacca ccgaggtgct gcgcgaactc cgcgaactcg tcaacaacgc caaagccgtg
    10681 ccgatgtcgg cctcgtgcat ggtcaaccgc gcccaggccc tggacctgat cgagaaggcc
    10741 acccaggccc tttccaccga cgtgggcgag gctcagcggg tcacggcgac ctcgctggag
    10801 accctggaac gcgcccaggc cgaggccgcc cagatcatcg cgtccgcaga ggagaaggcc
    10861 agctaccttg ccagccagac cccggtgatg gaggcggcca agcgaaaggc cgctgctctc
    10921 gaagccaagg cgttggcgga cgcggaaggt ctgcgccgcg aagccgacgc ctacgtggac
    10981 acccggatcg ccaccttcga agccagcctg cagaagacca tggggcaggt gcgcaccatg
    11041 cgtgaacggc tggcgtcccg ctcgagcctc gacgacagca acacccagat gttgccgagg
    11101 gcgaaggcct gaccagcacg ttttggagac ggagcgcggc tcaaggtaga gtcttgcgtt
    11161 ggttatgacg ctgctcgatt accgcaatgt gcgaaaggaa acgtcgtgac tccccgcctg
    11221 cctgaccatc gttctgggct tgtccttgac acccatgagt tgacccgtcg ggccggagtg
    11281 ttacgggaag tcacccgggt caccgaagca cctgtggatc tcggcatcga aatgatcggg
    11341 gtgccacccg gctcacccgt cgacctcgac cttcggttgg agtcggtggt ggagggggtc
    11401 ctggtctccg gtaccgccac ggtccggctc cggggtacct gtgcacgctg tctggacgac
    11461 atcgacgccc aagtcgaggt cgacctgcag gagttgtact gctacccggg gaaggaactc
    11521 gacgatgccg aggccttgca catcgagggc gagttgatcg acctcgagcc cgtcctgcgg
    11581 gacgcggtgg tactcgaact gccgttcacg cccttgtgcc agccggattg cgccgggttg
    11641 tgccccgaat gcggggccaa cctgaatcgg gatccgcagc acagccatgc cgaacccatc
    11701 gatcctcgat gggctgagct ggaagcgtgg ccggctccgt cgaacgaatc gaagaacaag
    11761 gagtaacgcc gtggccgttc ccaagcggag aatgtcccgc agcaacacgc gggcccgccg
    11821 ttcccagtgg aagacctccg cgccgaccct ggtgacctgc gccaacgcgg cttgtggtgc
    11881 caagcacctg ccgcacaccg cctgcccgga gtgcggccag tacggcccgc gcgctgcccg
    11941 ccggcagatt ctcgagtcct gaaggctctc cggccacgtc cggtgatccg gcggcaacga
    12001 gccgctgcct cgagctgatc gaagagctgg ggatccaact ggatccccag ctgtttcgcc
    12061 ttgccgtaac ccaccgttcc tacgcctacg agaacggtgg cctgccgcac aacgagcgcc
    12121 tcgagttcct cggcgacgct gtgcttggca tcgtggtcac cgagttcctc taccgaagct
    12181 ttcccgacct gcccgagggc cgactggcca agctccgggc tgcggtggtg aacgcgcaca
    12241 gcctcgccgg ggtggctcgt tcgctcgacc tcggcagtga gatcctgctg ggcaagggcg
    12301 aggtcgccac cggcggctcc gacaagtcct cgatcctggc ggactcgttg gaggctgtcc
    12361 tcggcgccgt actgatttct tccggacggg acgccgccga ccgcctggtg caccaccttt
    12421 tcgacccgct ggtgctggac gcggccgtgc tcggtgccgg tctggactgg aagaccagcc
    12481 tgcaggaact gagcgccgag ctggagctcg gggtgccggt ctaccgggtc accgagtccg
    12541 gccctgacca cgagaagcgc ttcgaagccg ccgcagtgat cggcgcgcgt gctttcggca
    12601 ccggttccgg cacctccaag aaacaggccg agcagggcgc ggccaagagc gcgttcgccg
    12661 cgctgtccga tgttgccgct gccaggtcgg agtcggcgct ttccggcgat tccgcctgat
    12721 gcctgaacta cccgaggtcg aagtcgtccg ctccggcctc gcccaactga ttcccggtcg
    12781 ccggatctcg gccgttacag tgctccaccc gcgtccggtg cgccgccacg tcggtggccc
    12841 ggacgacttc gcgtccgtat tggtcggacg gaggttcgcc gaacccaagc ggcgcggcaa
    12901 gttcatctgg ctgccgttca ctgatgggga cgcgctgatg gcgcatctgg gcatgagcgg
    12961 ccagttccgg gtcgacgctc ccgaagctga gctgccgccg cagtgccgcg tccgcttcgc
    13021 cttcgacgat gggggaccgg agatccggtt cgccgaccag cgcatgttcg gtggcttgtg
    13081 gctcagcccg ggcggcgccg agctgccggt ggaagtggcc cacatcgccc gcgacctgtt
    13141 cgatcccgac ctcgaccggg acgcgttggt ggctgacatc cggcgcaggc acagtggcat
    13201 caagcgggtg ctgctgaacc agacggtcgt ctcggggatc ggcaacatct acgccgacga
    13261 agccctgtgg cgcgcgcggg tgcattacga cactccggcc gaccggctca gcccggcgtt
    13321 ggtcgtcgag ttgctggacg cggccgttgc ggtgatgcgc gaatcgctgg ctcagggtgg
    13381 cacgtccttc gacgccctgt atgtgaacgt caacggcagt tccggatact tcggccgctc
    13441 gctgtcggcc tacggcaggg aagacctgcc ctgcctgcgc tgcggccgcg ccatcgtccg
    13501 cgagcccttc atgaaccgct ccagttatcg ctgccctgcc tgtcagcggc gtccgcgcac
    13561 ccaggtcgtt cgatgatgtt cagcgctgag cgggaagacg ccccacgtcg agccgtcgcg
    13621 cgctgcacgc agcctctcgc gtattgttcg cagatgtgac cggagccgaa ccgacccagc
    13681 gcttccagcg cgtgctgcgg cagttcgtga agttcggcat cgtgggcggc gccggcgtgg
    13741 tagtgaacat gctggtcgcg atcgccatga acaaggccaa cggcggcacg attaacgccc
    13801 agcgcatcct gttcgccatt cctggcacca acttcaactt ccgctacacc gtgctggtct
    13861 ggctggtcgc cttcctggtc gccaacgtct tcaacttcca gctgaaccgt tcctggacct
    13921 tccggcacac ccgcaaggca ccgtggtggc aggagttctg gccgttcctc gcggtcggtt
    13981 cggccgccgc gctgctgggc atcttcatca aggtcgcctt caccaacccg acctcgtggt
    14041 tctacctgcc cgagccctgg ttccatgaga acgccgggtt cgcctcccgc gagtactggg
    14101 cgcagctgtt caccatcgtg atcacgatgc cgatcaactt cgtggccaac aagctgtgga
    14161 cattcacgca cgtgcgcaac aagcaccaag cggcggtgta gtcgatgtac ctgaagagcc
    14221 tcaccctgcg cggcttcaag tcgttcgcgt ccaccaccaa cctggtcttc gagccgggaa
    14281 tcacctgcgt ggtcggaccc aacggctccg gcaagtcgaa cgtcgtcgac gcgctcgcct
    14341 gggtgatggg cgagcagggg gccaagtcgc tgcgtggcgg caagatggag gacgtcatct
    14401 tcgccggcac ttccggacgc gccccgctcg ggcgcgccga agtggtgctg accatcgaca
    14461 actcggacgg ttcgctgccg atcgactaca ccgaggtcac gatcagccgg acgatgttcc
    14521 gcaccggcgg gtccgaatac gccatcaacg gttcgcccgc gcggctgctg gacgtccaag
    14581 acctgctcag cgactcgggt atcggccggg agatgcacgt catcgtcggc cagggtcagc
    14641 tcgattcgat cctgcaggcc acgcccgaga cccgtcgcgg cttcatcgaa gaggccgccg
    14701 gggtgctcaa gcaccgcaag cgcaaggaaa aggcacagcg caagctcgaa gccaccgcgg
    14761 ccaacttgaa ccggctctcc gacctgctcg gcgagatccg ccgccagctg aagccgctag
    14821 gccggcaggc cgaggtggcc cgcaaggctg cggtcatcca ggccgacctg cgtgacgcgc
    14881 gcgcccggct gctggccgac gatctggtcg ccgccacttc gtcgctggcg accgatctgg
    14941 ccgccgaggc cgaactcaag caacgccgac tgcggatcga ggcggagctg acctccgccc
    15001 gtgaggccga gcaggctgcc gaagtcgccg cacgagacgc cctgcccgcg ctgaccgcag
    15061 cccaggagac ctggtacgcg ctggccgggt tggccgagcg ggtggcgagt acgacgacga
    15121 tcgcgtccga acggctgcgc aacgcggcag cgatcggcac cgacgagcgt ccggggcgcg
    15181 atccggaggc cattgagcgc gaggcggccg agatccgtaa aaccgaggcg tccgtgcagg
    15241 ccgaggtggc cgaacgcacc gcggcgctgg cggccgcgac cgatgcccgc aacgccgccg
    15301 agacagcgca cgccgaagcc gagaagcagt attcggccca actgcgagcg atcgccgacc
    15361 gcagggaggg cctggcccgg cttgccggtg aagtgaacac actgcgcagc cggtccgagg
    15421 cagccggggc ggagatcgag cggctggtgg cggccaaggc cgaagctgag gaccgggcca
    15481 ccaacgccaa gcggcaattc gctcagttgg agacccggct cgccgggttg agcgccggag
    15541 agtccgggct ggacgatgcc tacgaagccg ctgaggctga cgtggctgca cggctgaccg
    15601 cactggaaga gctccagaca gccgagcggg cggccaccgc ggagcgggga gccgcagcgg
    15661 cgcgcgccga agcgctggcc ctcggcctgg agcgacgcga cggcaccgcc gccctcctcg
    15721 ccgccggtga ccagctggac ggcctgctgg gctcggtcgc ggcactgatc agcgttccgg
    15781 tcggcaagga ggttgccatc gcggccgcgc tgggttcggc tgcggacgcg atcgcggtga
    15841 ctggggtgga cgccgccgtc cgcgccttcg accacctgaa agccgaggac ctgggccggg
    15901 ccggcatgct gctgggcgct gacctgcccg cggacacgtc cggctggccg aagctgccgg
    15961 cgaacgcctg ctacgcggcc gggctggtgg actgcgcggc gagcctgcgt ccggccattg
    16021 aacggctctt gttcaaggtg gcggtcgtgg acgacctggc ccagggccgc gagcttgtgg
    16081 ccaagctgcc ggatgtgacc gctgtgacca ccggcggcga cctgctcagc tcctggttcg
    16141 cggccggcgg atcgtcggca aagccgtccc tgctggaggt gcaggccgcg ctggatgaga
    16201 ccaacgaaaa gctcagcgcc gctcaggccg aggtcgagcg gacccggttc gcgatcgccg
    16261 aggccgagca gttgctcgcg tccgctcgcg aacggtcgga cgcggcactg gcccgcctgc
    16321 acgaatcgga cgccgagcat gccgcgatgg ccgaggaggc gggcgagctg aaccagatcg
    16381 tccgctcggc ccaggccgaa gccggacgcc ttgcgcaggc ggccgacaac gcggccacgg
    16441 cccgcgacca cgcgctggcc aacctgagcg agcttgagac gcgactggaa ctggcctccg
    16501 agcagaccga gaccggtgag gccgatccga ccgaacgcga ccatctcggt gacctggccc
    16561 gcgcggcccg cgccgccgaa atggaggcgc ggctggcgtt gcgcacggtc gaggagcggg
    16621 cacgagcctt cgccggacgg gcggacagcc tgctgcgggc agccgatgcg gagcgctcgg
    16681 cccgggccaa ggccgccgcg cgtcgggagc ttctccgtcg ggaggcggag gtggccgccg
    16741 gcgtcctgaa ggccgccgaa tggctggccg aacaggtgag cgcgtccagg gctcttgccg
    16801 agaccgaacg catgcgtgcg caggaggctc gcagcgttgc cgagaccgag ctgaccgccg
    16861 tccggcagcg cgggcgggaa ctggcgaaag cgttcgagaa cctggtcgac accgtccacg
    16921 ccgacgaact cgcccgcgcg caacagcaga tgaagatcga cgcgctggcc gagaaggcgc
    16981 tggccgaact cgggctggag ccggaggcgc tggtcggcga atacgggccg gaacaactgg
    17041 tgccggtgct ggtcaacgcc gacggcaaac cgctgaccga ggacgacccg cagccagatc
    17101 ccgtcccgta cgtgcgggcc gagcaggagc ggcggctgcg ggcggctgag cgtgacctcg
    17161 cggtgctggg gaaggtgaac ccgctggcgc tggaagagtt cgacgcgctc agcgagcggc
    17221 acactttcct ggccgagcag ctggctgacc tgcgcaagac ccgcgacgac ctgaacggga
    17281 tcatcgccga cgtggacacc cgcgtgcagg aagtgttcgc ggccgcctac gccgacgtcg
    17341 aacaggcctt cgaggacgcg ttcgcccggc tgttccccgg cggcgaggga cgcctcgtgc
    17401 tcaccgagcc gggggagtgg ctgaccaccg gtgtggacgt ggaggcacgt cccgccggga
    17461 agaaggtgaa gcggctgtcg ctgctttccg gcggcgagcg ttcactggtg gcggtggcct
    17521 tcctggtggc gctgttcaaa gcccgtccca gccccttcta tatcctcgac gaggtcgagg
    17581 ccgcgctcga cgacaccaac ctcggccgcc tgctggagat ctacgaagag ctccgggcga
    17641 acagccagct gctggtgatc acccaccaga agcgcaccat ggagatcgcc gacgcgctgt
    17701 acggggtgac catgcggtcc gacggggtct cggccgtgat cagccagcga ctgcgggatg
    17761 cctgagccca gccaacgtca tcccgacgca ggtcggcatc tctgaaaatt cgccacaagg
    17821 agactgagaa ctcgaggagg ctcaactgag ccttccgccg cgtgcggcat actgttcgta
    17881 gtccatcgga aggctgtgtc ttgagttatt ggatcatcac cgtggcgctc gtcgcgatcg
    17941 ccctgtgtgt gatcgcggtg gtcgccgtcg gcatgaacgg cagcctcgcc agcaaagccc
    18001 ccgaattcgc caacgtgatg gccaacaccg cgaagcacct gaacggcgac gcggacccgc
    18061 cacaggggct ggtcgagttc ttcgaggaga tcgaggacat ccggcccaag agcgcggatt
    18121 cggccgcaga ggcgcccaag cgctgacttt ggctgggtgg tgggcctggt gttgcctacc
    18181 atgagcggcg tggagaacta cttctggtac gtcctggctg cggtcgcggc cgttctgatc
    18241 gtcgctgccg tcactatcgt cgttggtaac cgcaagggcc tgccgccggc cgagcccacc
    18301 ccggaactgc cttcggcgga gcgcgaggag ttcggcgaac cggttgaacc ggcgccagcg
    18361 gaagtgattc cggctgctcc ggccgagccc tcggcgccgg tgctcgagac gcccgaaccg
    18421 ccggcttccc ggatgtcccg gctgcggcgc aagctgtcgg ccagcaacaa tcccttcgcc
    18481 cgcggcctgc ttggtctgct cgcggtcgac aagttggacg cggacgcgtg ggacgacttg
    18541 gaggccacgc tgatcacgtc cgatctcggc gcgggcccga ccgatgagtt gatcaccgcc
    18601 ttgcgccgcg agttgtcact cgacgcagtg tccgaccccg acaaggccaa gcaggtgctg
    18661 cgtaccgaac tgctgaagct ggtcgccacc accgctgatc gaagcctcaa cgtgactggc
    18721 gcggacggcc tgcccggtgt ggtgctggtg gtcggcgtca acggcaccgg caagacgacc
    18781 acgatcggca agctttcccg cgtgctggtg gccgaggggc attcggtggt gctggccgcg
    18841 gccgacacct tccgggcggc cgcggccgac cagctgcaga cctggggaag ccgggtgggc
    18901 gtcccggttg tgcgcggtcc ggagggcggc gacccggcgt ccgtggcttt tgacgcggtc
    18961 gaccagggca tcgcgagtgg cgcggacgtg gtggtggtcg acaccgctgg ccggctgcac
    19021 accaagaccg gcctgatgga cgagctcggc aagatcaagc gggtgatcga gcgcaaggct
    19081 ccggtgaccg aggtgctgct ggtgatcgac gccaccaccg ggcagaacgg gctcgcccag
    19141 gcgcgggtgt tcgccgaggt ggtggacgtc accggcatcg tgctttcgaa gctggacggc
    19201 tcggccaagg gcggcatcgt ggttccggtc cagcgggagc tgggcgtgcc ggtgaagctg
    19261 atcgggctcg gcgagggcgc cgacgacttg gctccgttcg aggccgaagg cttcgtcgac
    19321 gcgctgctgg cgcagtgacg accatccgtc cggcccgacc ggaagacctg cccgcgctgc
    19381 tggagatcta caacgactcg gtggtgcgcg acaccgcgag ctgggatttg ttgccctgga
    19441 gcccggtgca gcacgccgag tggtatgcga ccaaggtcga gcacggctgg ccggtgctgg
    19501 tcgccgaagt ggacggcgaa gtcgccggct acgcgaccta cgggcagttc cggccgaaag
    19561 ctggtttcac cctgagcatg gagcacagcg tgtacctgcg tccggcgttc cagggacggg
    19621 gtctgggtcg tgcgctgatg acggcgatca tcgcggcagc gcgcgagaac ggcgttcacg
    19681 tcctgatcgg cggcctgagc gccgacaacg acgcttcatt cgccttgcac cgctcgctcg
    19741 gcttcgtcga ggtcgggcgg ctccccgagg tgggccgcaa gttcgaccgc tggctcgacc
    19801 tggtgttcgt gcagctaacc ctctgaaccc atccgtcccc atccgttcac ctcaaccggc
    19861 agcaaggtgg acggtttgct ggcgtctcgg cgagaaacgg tccaccttgc tgcgggcggg
    19921 tgatggggac gcttctccga ggccgatgac gctaccggta ggatgtcgga gttcccgaca
    19981 cgcgtgaggt accaccttgt tcgacaccct gtccgatcgg ctttcagctg cgttccgcaa
    20041 tctgcgcggc aagacgcggc tgtccgatgc cgacatcgac gcgaccgcgc gcgagatccg
    20101 gctcgccctg ctcgaggcgg acgtcaacct gggcgtggtc aaggacttca tcgcagcagt
    20161 caaggagcgc gctcacggcg ccgagattca tgccgcgttg aacccggcgc agcagatcgt
    20221 caagatcgtc aacgaggagc tgatcgcgat cctcggcggc gaggctcggc cgctgcgctt
    20281 cgccaagcgt ccgccgacgg tgatcatgct ggcgggcctg cagggctccg gcaagaccac
    20341 gctggccgga aagctcggca agtggctgaa ggaccagggc cattcgccgc tgctggtcgc
    20401 gtctgacctg cagcgtccga acgcggtgaa ccagctgcag gtggtcggcg agcgggccgg
    20461 agttcatgtc ttcgcgcccg agcccggcaa cggcatcggt gacccggtag gggtggcgcg
    20521 cacctcgatc ctgcacgcga tgagcaagct ctacgacgtc gtgatcgtcg acaccgccgg
    20581 ccggctgggt gtggacgccg aactgatgca gcaggctgcc gacatccggg acgcggtcaa
    20641 cccgaccgag atcctgttcg tcgtcgacgc catgatcggc caggacgcgg tcaacaccgc
    20701 ccgggcgttc gccgacgggg tcggcttcga cggggtcgtg ctctccaagc tcgacggcga
    20761 cgctcgcggt ggcgcggcgc tgtcgatcgc ccgggtgacc ggcaagccga tcatgttcgc
    20821 gtccaacggc gagggcctgg gcgacttcga cgccttccac cccgaccgga tggccagccg
    20881 aatcctcggc atgggcgacc tgctcagcct gatcgaacag gccgagaaga ccttcgacgc
    20941 agaggagtcc gcgaaggccg cgcagaagct cctgcagggt aagggcggcg agttcggcct
    21001 ggacgacttc ctgtcgcaaa tgcaggcagt gcggcggatg ggtccgctgt cgaagatcct
    21061 gggcatgatg cccgggatgg gccagatgaa ggaccagatc aacaacatcg acgagcgtga
    21121 gatcgaccgc ctggaggcga tcatctactc gatgaccccg gctgagcggg ccaacccgaa
    21181 gctgctggac ggttcccgcc gcgcgcggat cgcaaccggc tcgggcacca aggtctcgga
    21241 cgtgaattcc ctggtcaacc gcttcttcga ggcccgcaag atgatgcaga gcctgggtgg
    21301 cgcgatgacc ggaatgggcg gacgccggcc cgcggccaag cagcagccca agaagaagaa
    21361 gggcaaccac aaggtttccg gcaacccggc caagcggacg ggtggtggcg ctgcggctct
    21421 gcccccagca gcgtcggcgc ccacgtccgc gttcggcctg cccacgcagc cgaccgaggc
    21481 cgacctggcg aaggcggtcg gcgacttcca gcttccggcc gaactgcagc aactcttcaa
    21541 tccgaagaac gccggccccc gctgacgttc gcagtcgcgg ctggtcgcgt ccatacttca
    21601 ccggtcggct tcacgcgcct gatctgactc ggcccccgct tcggcttggt cgagctagac
    21661 gacgagcggt cagccgatgg tggcgttggc gtcttgacct agggatgcac cagaggccga
    21721 aagacagtcg agaccacccg tggccgccga catgtcgtga tctcgtagtc ggatctgcgc
    21781 gctggcccga ttctccggtg tctggctgag ctggctgcga ctttgcgaca cctgtgccgc
    21841 cgcgctctcg gttgacgagc ccgcgaccgc aataatgact cgatgagcca ccaccacgcg
    21901 ccgccgggat tgggagagcc ggagttcttc gcacacgatc acggtggccg cgagtccccg
    21961 tcgactcgcc ccggataccc gctgcaccta cagggggtac tgctccccga aggcgagccg
    22021 cgcgacctgt gggtggccga tggtctgatc cgcaccgaac cggtgcccga tgcggtgacg
    22081 gtctgccagg gcgcctggat cctgcctgga ctggtcgacg cgcattgtca catcggtctc
    22141 ggcgcgaacg gcgcggtccc ggacgaggtg gccgaggcgc aggcgctcac cgatcgggac
    22201 gccggcactc ttctgatccg cgacgcgggt tcgccgatcg acacccactg gatcgacgag
    22261 cgcgaagacc tgccccggat catccgggcc ggccggcaca tcgcccgtcc caagcggtac
    22321 atccgcaact acgccgcgga agtagagccc gaggagctga tcgccgaggt ggcgcggcag
    22381 gccgtccgcg gtgatggctg ggtgaagctg gtcggcgact ggatcgaccg cgaccgcggt
    22441 gatctcgcgc cattgtggcc ggcggaggtg gccgccgctg cgatcgaacg cgcgcatcag
    22501 ttgggcgcac gggtcaccgc ccactgcttc ggcgaggagt cggtgcagca actggtgcgc
    22561 gccggcatcg atggcattga gcacggcacc ggcctggacg cggccaccat cgagttgatg
    22621 gccgaacgcc gggtagcgct ggtgccgacg ctggtcaatc tggagaactt ccctgccttc
    22681 gctgatgccg gcgaggcgaa gttcccggcc tacgcggccc acatgcggac gctgcacgcg
    22741 cgccgtttcg agaccatcgg cgcggcgatc gaggctggcg tcccggtgtt cgctgggacg
    22801 gacgccgggg gcagcatcag ccacggcctg gtcggaaccg agatcggcat gctggccaag
    22861 cttggcggcg cggagttcgc gctgggtgcc gcgtcctggc gtgcccggga atggttgggc
    22921 gccccgccgg tggtcgacgg cgcctgggcc gacctggtgg tttacgcagc cgacccacga
    22981 ctcgatcctg gggtcaccag gcaccccagc ctggtgattc tccgcggacg tgttgtcgcg
    23041 tccgcacact gagcggccga acacacccgc aactcgcgtt tgccaccgaa ttgggcgctt
    23101 gccaccgaaa tttcggcagc gaacgccgac ttcggtagca aacgcgagtt gacgtggggc
    23161 gcgatgcgag ttgaggcggg gcgggatcgg acgcgggtag gcgccggggc tcagctgccg
    23221 cgggacagcg cccaccagat catcaccagg ttgagcccga tgatcagggt gacgatcagc
    23281 cagttgagcg cccgctgcca cgcacggtct acgaactcgc ccatcagccg gcggtctccg
    23341 gtcaccaagg ccaacggggc ggccgccagg gccacgccga agctgagcac gatctgactc
    23401 cagatcaaca ccgaagtggg ggagacgccg gtcagcagca gtagcaccgc cggtccgatg
    23461 gtgatggccc ggcgcagggt cggttccacc tgccacacgt gcatgtcctt caggatccgc
    23521 gcccccgcat gcgtgccgac cactgccgag ccgatgccgg acgcgagcag gccgatcgcg
    23581 aacacagccg ccggccacgg gccaaggtcg gtgccgagcg tcacgtgtat ggcctcgatc
    23641 gggtcgccga ccacgcccgc cagcgccacc gcgcccagca gcagcatcga gatgttcacc
    23701 gagccggaca gcaccagcgc gaggcccacg tccagcttct gtgctcgcag caatcggcgc
    23761 agcggacggt ccaggtgtcc gtccggacgg tgccgatcga tcgccagcgc cgagtgcagg
    23821 tagatggcgt gcggcatcac ggtcgcgccg agcatcgccg cgatcagcgg ccacgcgtcg
    23881 cggtcgggca cggagggcca cagcccggcc agggtcgcgt cccaagaagg tgccttgaag
    23941 aacagcgcgc cgacgaagcc gagcgcgacc actgcgagca gtgcgccaac accggcctcg
    24001 aacacgccct cgccgcgcga gcgcagcacc cacagcagca ccatcgccac gacgccgacg
    24061 acgatgctgc ccgcccacag cggcagcccg aacagcaggt acaggccgag cgcaccgccg
    24121 accacttcgg ccaggtcggt cgcgatcgcg atgacgaatg cctgcagccc gtagccgacc
    24181 gtcagcggac gctgataccg atggccggcg aaacgatcgg ccatggcctg gggcagggtg
    24241 gcgccggtga ccaggcccag cttcgccgac tggtactgga tcaccattgc catcagcgac
    24301 gccaccgcca gcacccacac cagcgcatag ccgtagtcgg cacccgcgct cagattggcg
    24361 gcaacattgc ccggatcgac gtaggcgacg ctggcaacga aagcgggccc gagcatgccc
    24421 agcagaccca atcgcgcctt gtcgcggggc cgatctgccg agtcggggcg gaacatgatg
    24481 ggggcaggct agccggttgg cccggtgagg acacgttctg gcacactagt aggcgcatct
    24541 ggctcggtcg gcgccctcta actccggctg atccggatcc agccgagctt cgccgtccgt
    24601 agccccacta cgaatagcgg agttcacccg tccgggccgg tggtgtcacc gtcccaagtt
    24661 ctgcacagga gaacccacac tcgtggctgt caagattcgt ctcaagcggc tcggcaagat
    24721 ccgctccccg cactaccgca tcgtcgtcat ggatgcccgc gccaagcggg acggccgggc
    24781 gatcgaggag atcggcctct accacccgaa gaacgacccg tcggtgatca ggatcgactc
    24841 cgagcgcgcg cagtactggc tcggtgtcgg cgcacagccg accgaggccg tcgtcgccct
    24901 gctcaagcgc accggtgact ggcagaagtt cagcggtgac accagcccgt ccggtgtcac
    24961 cccgcagccc gagccgaagg acaaggtcgc gctgttcaac gccgcacttg ccgaggccga
    25021 cagcgagccg gccactgccg cgatctcgaa gtcgaagaag aaggctgccg aggctgatgc
    25081 cgaagccccg gccgaggccg aagaggcctg agcatgctgg ccgaagcgct cgagcaccta
    25141 gtgcggggac tggtccccaa tccggacgat gtccgggttc gcgagtcgga tcggggccgg
    25201 acgcgcacac ttgaggtgcg ggtccatccc gaggacatcg gcaaggtcat cggacgccag
    25261 ggacgcaccg ccggtgccct gcgcacggtg atcgccgcac tggccggacg cgagcaggta
    25321 cgggtcgact tcgtcgacgt cgaccgccgc cctcgccggc gcagttgagc cgtcgcattg
    25381 agccaagcca ggttccgccc caccgcatca gtggtggggc ggaactgttt ggcgcacaat
    25441 cacgtcgtgg agccagaatg actgaccatt tgatcgtcgg gacggtcggc cgtgcccacg
    25501 gactgcgcgg ccaggtgacc gtccgtccgc gcaccgacag catcgacgaa cggttcgcgt
    25561 ccggggcgaa ggtcagcgcc ggcgatcggg aactcaccgt gaccggccac gggctgcagg
    25621 gcgggcggct ggtggtgagc ttcgccgagg tgcgcgaccg caccgcagcg gatgccctgc
    25681 gcggggtcga tctgtgggtc cagggcaccc cgaccgacat cgatgacgac gagttccacg
    25741 acagcaccct cgttggcctg cgggcctgcg acccggccgg ggccgaactc ggtgaggtgg
    25801 tcgcggtact gcacaacccc gctcaggacc tgctggtgat ccgtacgccg cttggtgagc
    25861 gcctggtgcc gttcgtcacc gaactggtgc ccgaggtcga cccggcagcc gggcaggttg
    25921 tgatcaatcc gatccccggc ctgctgagcg atgtcgaggt gcgcgatgcg gattgatctg
    25981 gtcagcatct tctgcgacta tttcgcgccc ctgcagctct cgctggtcgg caaggcaatc
    26041 agttccggca tcgtgacgct gggcgtccac gatctgcgcg actggacgca cgaccggcac
    26101 cgcaccgtcg acgacacccc gtacggcggc ggggccggca tggtgatgaa gccggagccg
    26161 tggggcgagg cgctggacga gctggtgccc ccagacggac cgaagccgct actggtggtg
    26221 cccactccgt ccggggtgcc gttcacccag gcgttggccc acgaactggc cggacgcgac
    26281 tggctgctgt tcgcctgcgg gcgctacgag ggcatcgacg cccgggtggt ggcgcacgcc
    26341 acgtcccgca tggaggtgct cgaggtcagc ctcggcgact atgtgctcaa cggcggcgag
    26401 gtggctgccc ttgcgatcac cgaagcggtg gtccggctgc tgcccggtgt cctcggcaac
    26461 ccggagtcgc tggtggagga gtcccacggc gacgatctgg acggcctgct ggagtacccg
    26521 gtgtacacga aacccgccaa ctggcggggt ctggaagtgc ccgcggtgct gctttcgggg
    26581 caccatggcc aggtctccga atggcggctg gcgcagtcgc gcgaacgcac cgcgcaacgc
    26641 cgtccggacc tcctggaggg gtcgaattag gcaacgctag gtttgcctaa aagcgctttt
    26701 tcgactgttc gtccagtgcg ggtccagatt gtgagacctg ccacactcaa aacccgctga
    26761 cagcacgaaa tgcggcggtt ttcttactag ggtggcgccg tggcaacaac aacattgact
    26821 cctcatcaga gggctctgcg atccacggtc actctcaagg taatcatggc tgtgaccggc
    26881 ctgctcctga tcgctttcct gctgatgcac gcgttcggca attccaagat gttcctcggc
    26941 gaggactcct tcaaccacta tgcagagtgg ctgaagggcc agaccgaaga cggtggcatc
    27001 ctttacccgc tgctgcccgc cggctggttc atctggatct tccgggtggt tctgctggcc
    27061 gcggttgtgc tgcacatcta ctcggctgtg acgctgtgga atcgcgcgct ggcggccaac
    27121 ccgtccacct acgcggcgaa gcgcgaccta gcccgtacct actctgcgcg caccatgcgc
    27181 tggggtggca tcatcctggc cggtctgctg atcttccact tggcgcagtt cacggttcac
    27241 gccgcgctta ccggtggtga gtacggcgcc gattacgcca gttcgccgta caagatggtg
    27301 atcggcgagt tcagccagtg gtggatggtg ctgctctacg cggtctggat ggtggcggtg
    27361 tgcatgcaca tccggcacgg cttctggagt gcgttcgcga cgctgggcgc caacaccagc
    27421 gcgaaggctc gcgcggtgct gaacggctgc gcctgggccg tcgccctcat cctctacatc
    27481 ggcttcatga tcatgccggt ggccgttctc ttcggatgga tcaactgatg actacgacta
    27541 cgaaagcgcc cgcggccgcg ctgcccggcg ccgactactt caccctcggc gccaacatcg
    27601 ccgacacgaa ggccccggcc ggcccgatcg agggcaagtg ggccaagcgc aagttcgagg
    27661 ccaagctggt caacccgtcc aaccggcgca agctgaccgt gatcatcgtc ggcaccggcc
    27721 tggccggcgg tgccgcggcg gcgtccctgg gtgaggccgg ctacaacgtc ctcaacttct
    27781 gctaccagga cagcccgcgg cgggcgcact cgatcgccgc ccagggcggc atcaacgcgg
    27841 ccaagaacta ccgcaacgac aacgactcga ccttccggct gttctacgac acggtgaagg
    27901 gcggcgacta ccgcgcccgg gagaccaacg tctaccgcct ggccgaggtc agcgcg
//
LOCUS       DAOPRQ010000033        27883 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_1363_length_27883_cov_12.537085, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000033 DAOPRQ010000000
VERSION     DAOPRQ010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 27883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27883
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1363_length_27883_cov_12.537085"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(99..272)
                     /locus_tag="PKJ61_06040"
     CDS             complement(99..272)
                     /locus_tag="PKJ61_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13418.1"
                     /translation="MNEKSREDSLRQLGWVVVRWNWQDLLNPSALRRRIEAAFEQANP
                     EKIRGHYRLTPTI"
     gene            complement(386..1699)
                     /locus_tag="PKJ61_06045"
     CDS             complement(386..1699)
                     /locus_tag="PKJ61_06045"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007078377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HOC13419.1"
                     /translation="MRISVIGCGYLGAVHAASMAELGHTVVGIDVDAAKIAALSQARA
                     PFFEPGLPELLARGIESGRLRFSTDPAQAADAEAHFIAVGTPQRRGEHGADLSYVDAA
                     VAALLPHVRPGAVVIGKSTVPVGTAAALAERLAAAQPEAALAWNPEFLREGFAVRDTI
                     EPDRIVYGLPDGAAGERAKQVLDEIYAVALAADTPLVTADYATAELVKVAANAFLATK
                     ISFINAMSELADVVGADVTKLADAIGYDARIGRRFLNAGVGFGGGCLPKDIRAFRARA
                     EELGLGDTLAFLKEVDAINLRQRERIVTLVAEALAGDVVGKRVTVLGLAFKPESDDIR
                     DSPALDVAVRLNGLGAKVTSTDPQAIANSRAKHPQLAYEPDTDLALADAEVVVLLTEW
                     PEYRTLDPDHVGGLASGRIVIDGRNALDPERWRAAGWTYVGLGRP"
     gene            1893..2723
                     /locus_tag="PKJ61_06050"
     CDS             1893..2723
                     /locus_tag="PKJ61_06050"
                     /inference="COORDINATES: protein motif:HMM:NF013431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HOC13420.1"
                     /translation="MPDPDLFPGVQIHHKGGTYAVLDAGAHVLNWTPPGQQPVLWESP
                     LARFEPGVAVRGGVPVIFPWFSAGLTGDRTPAHGFARTANWVRESVTDDVATTGRLQV
                     VHSLTADGFDSAPFDARLIAEFAADQLRVQLVVTNPGPDVFTYEEALHTYFAISDIGR
                     ISIDGLDGCAYQDKVTGTAETQAGGVRFSGRTDRIYTHAGEATIDDPGRGRRIHVGKE
                     GSANTVVWNPGRDLAMTQRDIGDYYAEFVCVEAANVGASAITLDPGETHAIAQVVRLS
                     "
     gene            2809..4317
                     /locus_tag="PKJ61_06055"
     CDS             2809..4317
                     /locus_tag="PKJ61_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016668293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HOC13421.1"
                     /translation="MSRIESELFRSKVTTAEAAASIIKHGMNVGFSGFTGAGYPKAVP
                     VALAAQIKAAHERGEEFMIGCFTGASTAPELDGVLAEVDGISFRMPYQSDPIMRNKIN
                     AGISGYADIHLSHSGPQTWAGFLGKLDIAVIEVTAITADGELIPSSSVGNNTTWLASA
                     EKVILEVNAWQSLDLHGMHDIYEGVALPPNRKPVPLTGAGDRIGSTTMKVDPAKVVAV
                     IETDAADRNTPFKPLDDESRLIASHLLDFLQNEVNQGRLPKNLLPLQSGVGNIANAVL
                     AGLLEGPFEQLTSYTEVIQDGMVDLLDSGKMTVASATAFSLSPEYANKMNDNAAFYRD
                     KIILRPQDISNHPEMIRRLGVISCNGLIEADIYGNVNSTHVMGSRMQNGIGGSGDFTR
                     NAYISSFVTPSTAKGGAISAIVPMVSHHDHTEHDVMVVITEQGLADLRGLSPRQRAKV
                     IIDNCAHPNFKDALHEYYDHACKVANGQQTPHDLKLAHSWHVRFLETGSMLP"
     assembly_gap    4475..4574
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4795..5319
                     /locus_tag="PKJ61_06060"
     CDS             4795..5319
                     /locus_tag="PKJ61_06060"
                     /inference="COORDINATES: protein motif:HMM:NF037150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HOC13422.1"
                     /translation="MRLFSDQGYEKVSLRAIARAADVDPALIHHYFENKADLFSRSVL
                     DLPLDAEGIVARVLEGPKETIGERMLTEVLSAWDQPGARDRFTAMLRAAVTNPGARRP
                     LSEFLAKELMLKVAEAQGHSNAKLRAQTAVSIVLGLALCRDVLQLTAMARARNSVLIN
                     SLGRALQTQLVDTW"
     gene            5521..5952
                     /locus_tag="PKJ61_06065"
     CDS             5521..5952
                     /locus_tag="PKJ61_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13423.1"
                     /translation="MAVTGVALLWIYWEAVANFANASWLMGQFVTTPGSLERVLLSVG
                     VTVIGLVIGTTNAIVGYYAWVGYRWTRIAAPISAGLTLSALVLNPLAWAAIPAAIAGA
                     GLLWLPPSARFFRTWQGRRHPGQAFAPPVVDVYYGPLPRYR"
     gene            5974..6519
                     /locus_tag="PKJ61_06070"
     CDS             5974..6519
                     /locus_tag="PKJ61_06070"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014847488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HOC13424.1"
                     /translation="MSSSQLLRSIGRAILDALGTRRPSRPAGTAPKPGPRRQSAKRTP
                     TATSTYPGDYTGPLQLTYAPHPGKLADPGEVVWTWVPYEEDHSQGKDRPVLIIGRDGD
                     WLLGLPLTSQDHDRDAAQEASEGRYWVDIGRGNWDSSGRVSEVRVNRIVRVAPDAVRR
                     IAGKLDPVRFDEVAEGVRRHA"
     gene            6670..8346
                     /locus_tag="PKJ61_06075"
     CDS             6670..8346
                     /locus_tag="PKJ61_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13425.1"
                     /translation="MTATQKASRRADEPRRNPVARFIRDLCDAPTGRRVPGTVGVSVF
                     AGVVSLLASGYLVPSGLSLAYEDTLAHLTIARRLFDTLNNPGLEQLGTVRLPAPHLLL
                     APFVLLLWAWQTGWGAAVLGALCLAATAAGSYRAASRWGYGLLPRLAVVMVIVLNPSM
                     LYLYSTALTEPVLIAATAGCFAGLSNLATKKRRLSPGEIAVFAGLPAAIAVLSRYEGW
                     AVALAGAIFVGLVSWKQHGHDLRRTADSVLGFVAVPVAAALWWVAFNWVMLGDPLAFI
                     FGPYSASALQEEMVAAGLSTKGDLGRTIATLNYAVGTTVGPLLSGIALFGLVLAMFAR
                     TPIRMLGYLATTLVTYWFMIVSLFLGQAIILNPATTDTSVWNNRYGMASILPMAALVG
                     LGLEVVVQGSRRLRRGFGRVVRLTIAGAVLVAVLGQAGWQLQAPDERALVLQEAHSQL
                     RDSAGPRAAAEWLGANYDGGMILLDESVAANSVLPLAGLPLRNYYLRASDQLFTEALA
                     NPTAHARWLWVSTDDSDAVRQALAGSTELRSAYRLAHSQPGVDLYRRIEA"
     gene            8343..9938
                     /locus_tag="PKJ61_06080"
     CDS             8343..9938
                     /locus_tag="PKJ61_06080"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOC13426.1"
                     /translation="MTTDERAELGAPTAAPVSLDAARLSAAQLLYEASERFADDHPEY
                     SAKPGLVRWQRYSPFVLAAVLVLGLLANWWVTLIVVLIAANLVFSINATFKVASTFFR
                     PIAQLQHRRIMKAEAAELAELGLDPNNLLARAGDLPIYTILVPVYHEANVIGRIIDNL
                     SHLHYPQGLLDILVLLEENDTETIEAARAAQPPASVRLLVVPDGEPRTKPRACNYGLL
                     FAKGEYVVIYDAEDRPEPDQLLKALAGFRHDDVKRRLSRSSRPLACLQAALNYYNADY
                     NVLTRMFAIEYAHWFDSMLPGMDAANVPIPLGGTSNHFLRSALVEVGGWDPYNVTEDA
                     DLGLRLAASGYRVDVIQSTTWEEATAQIGPWIRQRTRWIKGYILTAAVNLRRPLKWLR
                     GNGVAGGLTMFGLILGTPLAFMMYPLALGFTLGSWLLGPVFRLGLPAWLVDFGLFNML
                     AFTILMIAVSALAAWRRYNWRIAVFAVFNPVYWLLHSYAAWRAALQVFWAPHLWEKTP
                     HGLTEEYDDGIIETGGLPHSAST"
     gene            complement(9947..10738)
                     /locus_tag="PKJ61_06085"
     CDS             complement(9947..10738)
                     /locus_tag="PKJ61_06085"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOC13427.1"
                     /translation="MVYGLTLAEQSWTSPATLGLIIAGLALLAGFVAIERRTPHPLLP
                     LRILTEPTRAAAFSVQALLGVVGVGAMVYLALHLQLVLHLPPLLAGLGTLPFTVSLMA
                     AVPFAMRLMDRFGPRPQLIFGPLISALGTFWLTFVTADGSYWTQVLPGVVLMGVGMGF
                     TVVPLNNLALYRVAPHDAGVASAASTATNQLGGSIGLAVLTAVYVGFAGRSQAGGVDA
                     MVAGHSAVFAVGALVFASIALVAWLAVRPVAAVVGSPELVSPALG"
     gene            complement(10723..10968)
                     /locus_tag="PKJ61_06090"
     CDS             complement(10723..10968)
                     /locus_tag="PKJ61_06090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13428.1"
                     /translation="MRPAPGYGVEEMFADLNRLGVAKFELIGSMVFDQYPRPKAEEIA
                     AVRAAAEKYGVTINSYGGYLDKGKIAGQSASPPWSTG"
     gene            10956..11084
                     /locus_tag="PKJ61_06095"
     CDS             10956..11084
                     /locus_tag="PKJ61_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13429.1"
                     /translation="MPDASSFVSGSTVGPALTIMALAARTSAFIAEQHAVGALTTV"
     gene            11222..11893
                     /locus_tag="PKJ61_06100"
     CDS             11222..11893
                     /locus_tag="PKJ61_06100"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HOC13430.1"
                     /translation="MEILFDTANLDAIETMTPIYPVAGVTTNPTIIKAEGRIDFYPHF
                     RRIRSIIGRERTLHVQVLARDADGIIADAHRLLDQIDENVYVKIPTNEQGIKAMTALK
                     REGVRVTATAIYSKTQGILAIAAGADYIAPYFNRMESLDIDTSSTIKSLAKFIDRQGA
                     DCKIMAASFKNVAQVSAAFEAGAHAVTVHPTLLRAALATPDVNQAVDAFASDWLDTYG
                     TDSLP"
     gene            complement(12025..12846)
                     /locus_tag="PKJ61_06105"
     CDS             complement(12025..12846)
                     /locus_tag="PKJ61_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13431.1"
                     /translation="MNNLDDLIRSSLNEHTPATAPDGGLAGRAKAVGRRVRMVQAGGV
                     ALLAVVLSVGVIWGVTTLPGRSQSIVAASPQPGPASTSVAPSESAGSSPSATAVRPST
                     SADGKVRDVSKLGAPADGQEGEMLVNYFASPTGNFHCFISTVGAGCTGQDWDAGVEPS
                     RAVCKGSEGGVMGPEIWGTANAAWECGTDPHSFPFFYDSENPDESPVAWWDESFGESA
                     PWSVDPTQTLAILPYGKTLVAGDFSCTMAKAGVSCHNSATGSGFKASRAKVELNS"
     gene            complement(12843..13361)
                     /locus_tag="PKJ61_06110"
     CDS             complement(12843..13361)
                     /locus_tag="PKJ61_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008358108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="HOC13432.1"
                     /translation="MTAPSPTLELAPELDFESFVASHQVRLRRYALALTGNGSDADDL
                     LQTTLVKVYLAWNRMADHEHVVGYARTTMARAYVSAWRRWGSRVSPTEHLPEVTGSRS
                     DADVTSDRDLIWRGLDRLGRRQRAVVVLRYFEDYDLAAIADALGITQGTVKSQLSRAL
                     TNLREILGGVES"
     gene            complement(13423..14709)
                     /locus_tag="PKJ61_06115"
     CDS             complement(13423..14709)
                     /locus_tag="PKJ61_06115"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOC13433.1"
                     /translation="MSRTIIRDFHDDDLDGIVRLYEDAGHRSSRPVYALSEVIASCRE
                     DHAVVAVQGEQLVGAAVGRAAHAQGWIVFFGLTDDIAGAEVGAGLLDELEHRMAPLGL
                     GKLSIIVPQDADRLDGLTANGFEVRNHLRYLERQMPVQRRELDLLAEVGGRILPRNLW
                     TRVSGMRQEKQLLEERLVTPLANPDLADRFGVVPPSAVMLFGPPGTGKTTFARAVASR
                     LDWPFVELFPSRLSDTPGGLAAALRSTFEVIGHLEHAVVFIDEVEEIASRRGGIPPSP
                     TQGVTNELLKLVADFREHQGRLLICATNFVRALDAAFLRHGRFDYVIPIGLPDAEARA
                     AIWRRYIPAGVLAQIDSAALVAASEGFTPADIEYAARKASQEALARALRSGDDPSAGE
                     ELATADYLEALAGTRATVSAETVEDFLEDITTIARL"
     gene            complement(14782..15084)
                     /locus_tag="PKJ61_06120"
     CDS             complement(14782..15084)
                     /locus_tag="PKJ61_06120"
                     /inference="COORDINATES: protein motif:HMM:TIGR02607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HOC13434.1"
                     /translation="MDELKPMHNPPHPGEFITEVYLTPFGVSARQLAARLEVSPSTLT
                     RLLSGASRVSPEMALRLSKVLGRSAESWLAMQDAHDLWKTRRRVDLTRITRLELAG"
     gene            complement(15370..>16359)
                     /locus_tag="PKJ61_06125"
     CDS             complement(15370..>16359)
                     /locus_tag="PKJ61_06125"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HOC13435.1"
                     /translation="HWTDSPHSAVRSAADALLQSIELAVAAEELGADGAYFRVHHFAQ
                     QLGSPFPLLAAIGARTSRIEIGTGVIDMRYENPLYLAEDAGAADLIAGGRLQLGISRG
                     SPEQVIEGYRYFGYVPPEGKTDADMARQHADVFLQVLSGEGFAQPNPRPMFPNPPGLL
                     RIEPHSPGLRQRVWWGAGSRATAEWTAQQGMNLMSSTLLTEDTGVPFHELQAEQIQRF
                     RDTWAASGHDWTPRVSVSRSIFPLVDDRDRAYFGSGRDESDQVGFIDGGVARFGRSYA
                     AEPDRLIEELAEDSAIAAADTLLLTVPNQLGVEYNAHVLESLLTQVAPGLGWR"
     assembly_gap    16360..16459
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <16460..17782
                     /locus_tag="PKJ61_06130"
     CDS             <16460..17782
                     /locus_tag="PKJ61_06130"
                     /inference="COORDINATES: protein motif:HMM:NF012363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HOC13436.1"
                     /translation="VLDASAPGATPQRRPFAEVTTIPEPSFPGDSTLNVNVFTPAPGR
                     THAKLPVLVWIHGGGFFAGSPSSPWYDGRSFNRDGVVTVALSYRLGFDGFGWIADAPL
                     NRGLLDQLAGLRWVQDNIAAFGGDPDRVTIAGQSAGGSSVLALLASPLAKGLFTRVIA
                     HSGADLAITVEQAKSIGLRAAELVGVEPTRAGWSQLSEDAILDAQLTLMPMDGAPPAN
                     PSEYVAGVLAGGALGLPFIPSVGDEVLPFGIADALARGIGSDVDLMAGTVAHEFTVAA
                     AAAREAWAGLDPVRALVDGGVAPEVAAQYVAGHPELATTADVCGQLVTDFSFRVPTLA
                     CADAHAERTWLYDFRWPEPGTGMSTHCIDLPFAWDLLDAEGVPAALGPQPPQDLADEM
                     HAAWVRFIRDGDPGWPEWDGTNARVFGADAADTYRPEQVLTASLPPRP"
     gene            17802..18308
                     /locus_tag="PKJ61_06135"
     CDS             17802..18308
                     /locus_tag="PKJ61_06135"
                     /inference="COORDINATES: protein motif:HMM:NF024788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HOC13437.1"
                     /translation="MGVPRPASRVPLRECGRLLLRAPSTAGQQAFTTDREVTTLSGFL
                     SNLWDFVWMFFTIFVFFAWLMALFSIIGDLFRDRALSGWAKAVWLLLLIFVPFLTALV
                     YLIARGGGMAERAASQAKAQQNVTDDYIRSVAGAGPAQELTQAKALLDSGALSADEYA
                     ALKAKILS"
     gene            18326..19453
                     /locus_tag="PKJ61_06140"
     CDS             18326..19453
                     /locus_tag="PKJ61_06140"
                     /inference="COORDINATES: protein motif:HMM:NF037123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13438.1"
                     /translation="MIHYELPTTSDLQALGATRPDALSIYLATEPTPAGRELAFTSAK
                     SAVDQAVRSLRDAGRSHAEQEAFRARWDSVADDAELWGNLASSLVIFLAPDFADEYVL
                     PNSFDAHTHFGTSFDLGPLVRAVTTPQRAFALTLSSDGWNLWQATESSRAAELPLVGE
                     HAEDAADATNRTSIRGRQLLRRLGGDEGQKVLLDRYAQVVANSVREELGHLDRHAELP
                     LFLFANEPLASMVQAHGLPGEVVLVPGAADELRPDQIDAAIRERIGELTGSRLSRAAD
                     RLGDGFAAGLTSVDVAQVARAAAAGAVGTLYYDLTADVRGSLDPETGAVSFAADGDNL
                     LAEIALLVLANGGEVYAVRPGEIKAEIWNGQLLAGLRLPLV"
     gene            complement(19653..20660)
                     /locus_tag="PKJ61_06145"
     CDS             complement(19653..20660)
                     /locus_tag="PKJ61_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13439.1"
                     /translation="MADLTAAGTGAGGLGTAAARWSFVFVWACGVLQVLVDGSLAAEP
                     LAWAIALPAGLVGALLLTTPGARPLVLARLLWLHPLALLVVWAALASAESVGELAALN
                     FATYLMAFQIPRGNVVAGSVGSGLLIGAAVAWALPQSPDGPALAMLIGVPLGCVAAGV
                     MWRLVLKWIVRSERAHRGSAERSAERAVASAEAIAFSRAELAAIGDLVVPVLDRLANG
                     EVIDAELRTELARAEAAVRDRIRAPHLQHPELMAEVDRLRRDGVAVVLLGEPAAPDQL
                     VDARLAEACRTAIAPVTSGRVTIRTFPANRPAALSVVVQTEDEAVQVQWSAAGELVAS
                     G"
     gene            complement(20648..21802)
                     /locus_tag="PKJ61_06150"
     CDS             complement(20648..21802)
                     /locus_tag="PKJ61_06150"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOC13440.1"
                     /translation="MTTADEQARWDAIDRPLGWLIALGGCVLILDQTIVQTGRLADFA
                     PLWNAGALLVIAAIIGLAVSGMMLPRSALRTIWRTVPVAHLVLQSTWLVGFVGPDVDA
                     ARPWLWTVEPAVITLLLLIAKPVVAVAVGLGFSLVPALTSLLTLGRLTDEILIQTPNE
                     LGNVVYVAVFIGVRIQLERLHAGEREAQRQHERQVRAAARLEQHAVLARFVHDEVLSA
                     LSAGMHADGSPSQPLRRDARNAIEAISLSTGPEPEHDRPLTSTDALALLTASLRRIDA
                     AFTLETECRPSQHQAKVVRTIALAAGEALRNSVRHAGPDTSRRVEITLSATLIRVCVR
                     DDGVGYTPDPHSQRLGVQRSIVDRMADLGGSARIDTAPGRGTEVVLTWPT"
     gene            complement(21789..22433)
                     /locus_tag="PKJ61_06155"
     CDS             complement(21789..22433)
                     /locus_tag="PKJ61_06155"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOC13441.1"
                     /translation="MKAQIGMVDDHPAIMLGVGAILNAQPDLHVVAAAASVQELIAQT
                     QRLDCVLLDLRLADGSTPTANLAALTALAIPVLVYTSGDRSDLIREATRAGATGVLRK
                     SEPPDVVVGAIRSLLRGEPVVTAEWAAALDFDPDFATVHLSPREAEVLTLYASGETAE
                     RVAALLFISRETVHDHVRRIRAKYAAADRPALTKVDLYRRAVEDGLVPQGHDHS"
     gene            complement(22565..24256)
                     /locus_tag="PKJ61_06160"
     CDS             complement(22565..24256)
                     /locus_tag="PKJ61_06160"
                     /inference="COORDINATES: protein motif:HMM:NF018448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HOC13442.1"
                     /translation="MLGLGGVGLAPAFAVEPTPSDPPPISASAEPTEPDEPEASEPKP
                     TETVPEPTPSPTEPSEPSPTAGPTSAPSPTAGDFPAATAGPTEAGRDIPGNGVLEPHP
                     PAWQLANAELSWIIALVVLISTAVLVLLLARRPDPPEHQLIAPQPDVSIGPSDSAMVA
                     GLADLEAAGEAMIDAGYSVTGVRTALTDIARVNGQPATEIVVLPTALFVSTRRFGAVR
                     TGAVSAGHQELRLNQIDVLDDVIRAARTLPSEPGAIKAAVAGVRTLPSPYTATQRAVA
                     HVVLSAGLSVLLDGSWAGVAVAAVLGLVVGVVLLFTEPLGRQYQALVTVGLAFLVSTS
                     VFTLTQIGLDPGVLAALIAPLVTLLPGGLLTTGVIELATGQMMAGAGRLAAGSMQLIL
                     LAVGLVSGAALVGVPRVELVEAQNPIGPLGPWLAVAIFGIGIVIHQCGRPRSIGWILL
                     VLYVAYGGQVLGDVLFGGVLSGMIGAFAMTPVAYVVSRQQDGPAAFASFLPAFWLLVP
                     GSLGLVGVTSILDGDSTGLSTLVTTGATMVAIMLGILVGSAIGGRLTGRGTVAVV"
     gene            complement(24490..25512)
                     /gene="glsA"
                     /locus_tag="PKJ61_06165"
     CDS             complement(24490..25512)
                     /gene="glsA"
                     /locus_tag="PKJ61_06165"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF009020.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HOC13443.1"
                     /translation="MYLPDHPSAEPAERYVSTGRLPLDGVVGELLDQSLAQFRDTSDG
                     QLSRVYPVLAEADPDLFGLALVGVDGLTRFAGDAEVAFTVMSIAKPFVFALVSEEIGI
                     AEVRGLVGVNPTGLPFNSIAAVERADRGRTNPMVNAGAIATTSLVPGGDPSARWDWLV
                     DGLSRFAGRRLQVDADTLASARATNFRNRALAMLLQEAGALVGDPLVALDLYTQQCCL
                     AVTAVDLATMGATLADGGVNPLTGERVVSAAVARATLAVMSVAGLYEGSGDWLLDVGF
                     PGKSGISGGLVAVSPGKGALGAFSPLLDSDGNSVRGQLAARFLAAELGLDLFSSVPAR
                     SPAGRV"
     gene            complement(25512..27014)
                     /locus_tag="PKJ61_06170"
     CDS             complement(25512..27014)
                     /locus_tag="PKJ61_06170"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF013079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HOC13444.1"
                     /translation="MKTLDLDPIPSRNRSVHLDVVAAGSTMTADALAVPVLAGQPTPA
                     ILGGVDLAALARAGFTPDVGKAVPFPSVNQPVLVAVGVGDPEQLTVARLRDAAAAFAR
                     AVPQDAALAVRVPAIAQVPVADAAAAIVEGVLLARYQFSFKSVAAGPVAVESLVLEAP
                     EGAVDDVRAGATRGLASARAGMLGRDFASCPAGLLTASRLAELAVQIGAESGLKVELF
                     DRRALIELGCGGLLGVNKGSIEEPRMVVLRYTPSGESTGHLALVGKGIMYDSGGIALK
                     PADLSHSQMKNDMTGAGNILAAMSALAELGCRNTVTGYLMCTDNMPSGSAMQLGDVLT
                     IRGGKTVEVLNTDAEGRLVMADALVLAVEEGVDAIVDIATLTGACLRTLGEEVAGLLS
                     NDDALAAQVSAAGDAVDEPVWRLPLIRSYRAELDSDIADLKNLGGPNAGSITAGLFLE
                     EFVAGVPWAHIDIAGTAQSSRAHRWINRGTTAFGTRLLLELSCAFTPSAG"
     gene            complement(27047..>27883)
                     /locus_tag="PKJ61_06175"
     CDS             complement(27047..>27883)
                     /locus_tag="PKJ61_06175"
                     /inference="COORDINATES: protein motif:HMM:NF015746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HOC13445.1"
                     /translation="KVTMKRLGVYDHADYQGVDEEQIDEAAEAKGLKYSGWYLLGFAV
                     IIALLTAIPGAPLRDPETGNIIGNTPFMASLVFVISLAFMVCGLGYGKGAGTLQGGNE
                     AVTAIAKTFGSLGGLLLMFLMIAQFIALFNWSNLPTVAAISAAELLQQANVPAIVLLL
                     GFIVVILVLDLILPGLVPKWVIFAPVFIPIFASLNVAPQTLLAAYRVADSPLNVLTPL
                     MVYLPFMVTVAQRYKKDAGIGTIIALMVPYTLWITLAWVVLFVAWFLLGIPWGPGAPV
                     NI"
BASE COUNT         4597 a         9777 c         9140 g         4169 t
ORIGIN      
        1 aaccggcgtt tgctaccgaa gtggacgttt gctgccgaag tatcggtggc aaacgtccga
       61 ttcggtagca aacgcgagga aatggacgac agggtgggct agatggtggg ggtgagccgg
      121 tagtgaccgc gaatcttctc cgggttcgcc tgctcgaacg cagcctcgat tcgccgtcgc
      181 aacgccgaag ggttcagcag gtcttgccag ttccatcgca ccaccaccca accgagctgg
      241 cgcagcgagt cttcacgcga tttctcattc atcacggttc gggccacctg gtccgaaggt
      301 ccgatgtatt tcaccttgcc gtcgaactcc ccaagcacac ctagcggacg cggccgtgcg
      361 ggcggtcgca tcgggctcgc agcgatcagg ggcgacccag gcctacgtag gtccacccgg
      421 cggcgcgcca gcgctctggg tcgagcgcgt tgcggccgtc gatcacgatc cggccggacg
      481 ccagcccacc gacgtggtcg gggtcgaggg tgcggtactc gggccactcg gtgagcagga
      541 cgaccacctc cgcgtccgcc agcgctaggt cggtgtccgg ctcgtaggca agctgcgggt
      601 gcttcgcccg ggagttggcg atcgcctgcg ggtcggtcga ggtcaccttc gcgcccaatc
      661 cgttaaggcg gacggccacg tccagcgccg gcgaatcgcg gatgtcgtcc gactcgggct
      721 tgaacgccag ccccagcacg gtcacccgct tgcccaccac atcgccggcg agcgcctcgg
      781 ccaccagcgt cacgatccgt tcgcgctggc gcaggttgat cgcgtccacc tccttcagga
      841 aggcgagcgt gtcgccaaga cccaactcct cggcccgcgc ccggaacgcc cggatgtcct
      901 tcggcaggca gccgccgccg aaccccaccc ccgcgttcag gaacctgcgg ccgatccggg
      961 cgtcgtagcc gatcgcgtcc gccagcttgg tgacgtcggc gccgaccacg tccgccagct
     1021 cggacatggc gttgatgaag ctgatcttcg tagccaggaa cgcattggcg gccaccttca
     1081 ccagttcggc cgtggcgtag tcggccgtta ccagcggggt gtcggcggcc aacgcgaccg
     1141 cgtagatctc gtccagcacc tgcttggccc gctcaccggc cgccccatcg ggcagcccgt
     1201 agacgatccg gtcgggctcg atcgtgtccc ggacggcgaa accctcgcgc agaaactccg
     1261 ggttccaggc cagcgcggcc tcgggttgcg ccgcggccag ccgctcggcc aaagccgcgg
     1321 ccgtccccac cgggacggtc gacttgccga tcaccaccgc gcccggacgc acgtgcggca
     1381 gcaacgcggc caccgctgcg tccacgtagc tcagatcagc accatgctcg ccgcggcgct
     1441 ggggcgtgcc gacggcgatg aagtgcgcct ccgcgtccgc agcctgggcc ggatcggtgc
     1501 tgaagcgcag ccgtcccgac tcgatgccgc gcgcgagcag ctcgggaagg ccgggctcga
     1561 agaacggcgc cctggcctgg ctgagagccg caatcttagc cgcgtcaacg tcgatgccca
     1621 cgacagtgtg acccagttca gccatggacg cggcgtgcac cgcccccagg tacccacaac
     1681 cgatcaccga gatccgcatg gggtcggcct cactttcatc gctggcggcg tgatgtcgtc
     1741 gttcatcttg ccagtacccg gctcggctcg gcgtatgttg cagtgatggt gttctgtcca
     1801 agctcggcca gcgccaccaa gcattgatca gggttcccta gtcctgcaga accggctcga
     1861 ccagccgggg tttcggggcg taacctgcgg cggtgcctga tcctgacttg tttcctggcg
     1921 tccagatcca ccacaagggc ggcacctacg ccgtccttga cgccggcgcc cacgtcctga
     1981 actggactcc gcccggccaa cagcccgtgc tctgggagag cccgctggcc cggttcgagc
     2041 ccggggtcgc cgtccgtggt ggcgtgccgg tcatcttccc ctggttcagt gccggcctga
     2101 ccggtgatcg gacgccggcc cacggtttcg cacgcaccgc caactgggtc agggaatcgg
     2161 tcaccgacga cgtcgcgacc accggacgcc ttcaggtcgt ccactcgctc accgcggacg
     2221 gcttcgacag cgctccgttc gacgcccggc tgatcgcgga gttcgccgcc gaccagctcc
     2281 gcgtccagct cgttgtgacc aaccccggcc cggacgtgtt cacctacgag gaagcactgc
     2341 acacctactt cgcgatcagc gacatcggcc ggatcagcat cgatggcctc gacggctgcg
     2401 cgtaccagga caaggtgacc ggcaccgccg agacccaggc cggcggcgtc cgcttcagcg
     2461 ggcggacgga tcgcatctac acccacgcgg gcgaggccac tatcgacgat ccgggacgcg
     2521 gccgccgcat ccacgtcggc aaagagggct ccgcgaacac ggtggtgtgg aacccgggtc
     2581 gcgacctcgc catgacgcag cgggatatcg gtgactacta cgccgagttc gtctgcgtcg
     2641 aggccgcgaa cgtgggcgcg tccgcgatta ccctcgaccc cggcgagacg cacgccatag
     2701 cgcaagtcgt gcgtctcagc tgaaccgttg cagcaatcgc catctggtca cgagggcaca
     2761 ccgatttctg taacctgact ctgttccacc cagcgtcgag gagattcaat gtcccggatc
     2821 gaaagcgagc tctttcgcag caaggtaacc accgcggaag cggctgcgag catcatcaag
     2881 cacggcatga atgtcggctt ctccggcttc accggagccg gctaccccaa ggctgtgccg
     2941 gtcgccctgg ccgcccagat caaggctgcg cacgagcgcg gcgaagagtt catgatcggc
     3001 tgcttcaccg gggcctcgac cgctcccgag ctcgacggcg tcctcgccga ggtggacggc
     3061 atcagcttcc gcatgccgta ccagtccgac ccgatcatgc ggaacaagat caacgccggc
     3121 atctccggct acgccgacat ccacctttcc cactccggcc cgcagacctg ggccggtttc
     3181 ctcggcaagc tcgacatcgc cgtcatcgag gtcaccgcca tcaccgcgga cggcgaactc
     3241 atcccgtcct cttcggtcgg caacaacacc acctggctcg cgtccgccga gaaggtcatc
     3301 cttgaggtaa acgcctggca gagcctcgac ctgcacggca tgcacgacat ctacgaaggt
     3361 gtggcgctgc cgccgaaccg caagcccgtg ccgctgaccg gcgcgggtga ccgcatcggc
     3421 agcaccacca tgaaggtcga cccggccaag gtcgtcgcgg tgatcgagac cgacgccgcc
     3481 gaccgcaaca ccccgttcaa gccgctggac gacgaatccc gcctgatcgc cagccacctg
     3541 ctggacttcc tgcagaacga ggtcaaccag ggacggctcc cgaagaacct gctgccgctg
     3601 cagtccggtg tcggcaacat cgccaacgcg gtgcttgccg gcctgctgga gggcccgttc
     3661 gagcaactca cctcctacac cgaggtgatt caggacggca tggtcgacct gctcgattcc
     3721 gggaagatga cggtggcttc ggcgaccgcc ttctcgctta gtccggaata tgcgaacaag
     3781 atgaacgaca atgcggcgtt ctaccgcgac aagatcatct tgcggccgca ggacatctcg
     3841 aaccacccgg agatgatccg acgactcggc gtgatctcct gcaacggact gatcgaggcc
     3901 gacatctacg gaaacgtgaa ctccacccac gtgatgggct cgcgaatgca gaacggcatc
     3961 ggcggctccg gcgacttcac ccgcaacgcc tacatctcat ccttcgtcac cccgtccacc
     4021 gccaagggcg gcgccatctc ggcgatcgtg ccgatggtgt cgcaccacga ccacaccgag
     4081 cacgatgtca tggtggtgat taccgagcag ggcctggccg acctgcgtgg cctctcgccg
     4141 cggcagcggg cgaaggtgat catcgacaac tgcgcgcacc cgaacttcaa ggacgcgctg
     4201 cacgagtact acgaccacgc gtgcaaggtc gccaacggcc agcagacccc gcacgacctc
     4261 aaactggcgc acagctggca cgtccggttc ctcgagaccg gctcgatgct gccctgacct
     4321 gaacaagaaa ggggacggtt ctttttccgt tcagcttgtc cacagccccg cggcgcgaac
     4381 cgctcgctcg gtgtggacaa cctgaacaaa aaaaggaacc gtcccctttt cgttcgcctg
     4441 cggtggcgga cgcggacgcg gctcagccca gcagnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnncgaagt gacggcgtgt tcgtaagaag cgataattag cgggatagta
     4621 ttcaccacgg tcccatcgag atcgaaaacc gcgacgggcc agcaagcatt ggacatggtc
     4681 gtgacaatac gaccaactca gcagggggga atgcaggtga ccgaactggg acacaggcct
     4741 cgtggtcgcc ggcccggcag gctcgatacg cgcggagtca ttcaggacgc cgcgttgagg
     4801 ctcttctcgg atcaggggta cgagaaggtc tccttgcgcg ccatcgcgcg cgcggccgat
     4861 gtcgatccgg cacttatcca tcactatttc gagaacaagg cggacctgtt ctcgaggtcg
     4921 gtcctcgacc ttccgctcga tgcggagggc atcgtggcca gggtgctcga aggtccgaag
     4981 gagaccatcg gcgagcgcat gctcaccgag gtgctgtcag cttgggacca gccgggcgcc
     5041 cgggaccgct tcaccgccat gcttcgcgcc gcggtcacca atcccggtgc acgccggcca
     5101 ctcagcgagt tcctggccaa ggaactcatg ctcaaggtcg ccgaggccca ggggcactcg
     5161 aacgcaaagc tgcgcgccca gactgcggtc agcatcgtgc tcgggctcgc gctctgccgg
     5221 gacgtcctgc aactcaccgc gatggccagg gcccgcaact ccgtcctgat caactcgctc
     5281 ggccgggcac tgcagaccca actggtcgac acctggtgac gacgtaggct gggccgctct
     5341 agaggaggtg cgatgaccag ccagccgaca gctgattcga ctgcgatcga ggccatccgt
     5401 cctgcgatgg cttcgatcca gctgcctagc ccggatggcc tgacgccgga caacccccgc
     5461 accggcgaac cccagcgccc agccacaatc gtgatcgcga cagttcttca ctggctcgcg
     5521 gtggcagtca ccggagtcgc gctgttgtgg atctactggg aggcggtggc caacttcgcc
     5581 aacgcatcct ggctgatggg ccagttcgtc accacccccg gctcgctgga gcgggtgctg
     5641 ctctcggttg gggtgaccgt gatcgggctc gtgatcggca ccaccaacgc gatcgtcggc
     5701 tattacgcct gggttggcta ccgctggacg cggatcgccg ccccaatcag cgctggcctg
     5761 accttgtcgg ctctcgtcct gaatccgctg gcgtgggctg cgattcccgc agcgatcgcg
     5821 ggcgccgggc tgctgtggct gccgccttcc gctcgtttct ttcgtacctg gcagggtcgt
     5881 cgtcatcccg ggcaggcctt cgccccaccg gtggtggacg tctactacgg gccactgccg
     5941 cggtaccgct gaatccgcgc taccttcggg tgcgtgagtt ccagccaact gctccgatcc
     6001 atcggccggg cgatcctgga tgccctgggg acgcgtcggc ccagtcgtcc ggccggcaca
     6061 gcgccgaagc ccgggccacg ccggcagagc gccaagcgga cgccgaccgc gaccagtacc
     6121 taccccggcg actacaccgg gccactgcaa ctgacctacg caccgcaccc cggcaaactc
     6181 gccgacccgg gcgaggtcgt ctggacatgg gtgccgtacg aagaggacca cagccagggc
     6241 aaggaccggc cggtactgat catcggccgc gacggtgact ggctcctggg cctgccgctg
     6301 accagccagg accacgaccg tgacgccgcc caggaggcgt ccgagggtcg ctactgggtg
     6361 gacattggcc gcggcaattg ggattcgtcc ggacgggtca gcgaggtgcg ggtgaaccga
     6421 attgttcggg tcgcgccgga cgcggttcgc cggatcgccg gcaagctcga tcccgtccgc
     6481 ttcgacgagg ttgccgaagg cgttcgccgg cacgcctgac cccggcggga gatgccggca
     6541 gccatcgcca atcgggcttt ggcttggctg ttcaccgtcg acgcaggtgg cttttcgggc
     6601 ctgatgacgt accctgagag tcccatcccc aatctgcccc tcccagggtc ccgaagcacc
     6661 agaggacttg tgaccgccac gcagaaggcg tcccggcgcg cggacgagcc ccgccgcaat
     6721 ccggttgccc ggttcatccg cgacctctgc gacgcaccca ccggacgccg cgtgcccggc
     6781 accgtgggcg tgtccgtgtt cgccggggtg gtctccctgc tcgcgtccgg ctacctggtg
     6841 cccagcggcc tcagcctggc ctacgaggac acgctggcgc acctgacgat cgcgaggcgg
     6901 ctgttcgaca ctctcaacaa ccccgggctc gaacaactcg gcacagtccg gttgcccgca
     6961 ccgcatctgc tgctggcccc gttcgtgttg ctcctttggg cctggcagac cggttgggga
     7021 gccgcggtcc tcggcgccct ctgcctcgcc gcaacggccg ccggtagcta tcgcgccgcg
     7081 tcgcgctggg gttacgggct actgccgagg ttggccgtgg tgatggtgat cgtcctgaat
     7141 ccgagcatgc tgtatctcta ctcgacggca ctgacagaac ctgtcctgat cgcggccacc
     7201 gccggctgct tcgccggcct tagcaatctg gcgaccaaga agcgtcggct gagccccgga
     7261 gagatcgccg tgttcgccgg cttgccggcc gcgatcgcgg tgctgtcacg ctacgaaggc
     7321 tgggcggtcg cgctcgctgg ggccatcttc gtcgggctgg tctcctggaa gcagcacggc
     7381 cacgatcttc gccgcaccgc ggatagcgtc ctgggtttcg tcgccgtgcc ggtcgctgcg
     7441 gcactctggt gggtcgcgtt caactgggtg atgctcgggg atccgctggc cttcatcttc
     7501 ggtccgtact cggccagcgc actacaggag gaaatggtcg cagccgggct ctccaccaag
     7561 ggcgacctcg gccggaccat cgccacgctc aactacgccg tcggcaccac ggtcggtccg
     7621 ctgctgagcg gcattgccct gttcgggctc gtcctcgcca tgttcgcccg cacaccgatc
     7681 cgcatgctcg gctacctggc caccactctg gtcacgtact ggttcatgat cgtgagcctc
     7741 ttccttggcc aggcgatcat cctgaatccg gccaccaccg acacctccgt ctggaacaac
     7801 cgctacggaa tggcgagcat cctgccgatg gctgcgctgg tcggcctcgg cctggaagta
     7861 gtcgtgcagg ggagccgcag gctgcgtcga ggtttcggcc gagtcgtccg gctgacgatc
     7921 gccggggccg tactggtggc agtgctgggg caggccggct ggcagttgca ggccccggac
     7981 gagcgggcgc tcgtactgca ggaggcccac agccagcttc gggattcggc tgggccgcgg
     8041 gcggccgccg aatggcttgg ggccaactac gacggcggga tgatcctgct cgatgagtcg
     8101 gtggccgcca actccgtcct gccgctggcc gggctgccgc tgcgcaacta ctacctgcgg
     8161 gccagcgacc agttgttcac cgaagcgctc gccaacccga ccgctcatgc gcgttggttg
     8221 tgggtgagca ccgacgattc ggacgcggtg cgccaagcgc tggccggaag caccgaactg
     8281 cgcagcgcct accggctggc ccacagccaa ccgggcgtcg acctctaccg tcggatcgaa
     8341 gcatgaccac cgacgagcgc gccgagctcg gtgcaccgac cgcggcgccc gtttcattgg
     8401 acgctgcccg actctccgcg gcgcaactgc tgtacgaggc gtccgagcgc ttcgccgacg
     8461 atcatccgga gtactcggcc aagcccggac tggtgcgctg gcagcgttac agccccttcg
     8521 tactagcggc ggtgctggtg cttggcctgc tggcgaactg gtgggtgacg ctgatcgtgg
     8581 tgctgatcgc ggccaacctg gtcttctcca tcaacgccac tttcaaggta gcttccacct
     8641 tcttccggcc gatcgcgcaa ctgcagcacc ggcgaatcat gaaagctgag gcggccgagt
     8701 tagccgaact cgggctcgac ccgaataacc tgctggcaag ggcgggtgat ctgccgatct
     8761 acacgatcct ggtgccggtg taccacgagg cgaacgtgat cggacggatc atcgacaacc
     8821 tctcccacct gcactaccca caggggttgc tcgacatcct ggtgctgctc gaggagaacg
     8881 acaccgagac gattgaggcg gcccgggcgg cccagccacc ggcgtccgtc cggttgctcg
     8941 tggtgccgga cggagagcct cgcaccaagc cgcgcgcctg caactacggg ctgctgttcg
     9001 ccaagggcga gtacgtggtg atctacgacg cggaggatcg tcccgagccg gaccaactgc
     9061 tgaaggccct ggccggcttc cggcacgacg acgtgaaacg gcggctgagc cgtagctcgc
     9121 gtccgctggc ctgcctgcag gcagcgctga attactacaa tgccgactac aacgtgctca
     9181 cgcgcatgtt cgcgatcgag tacgcgcact ggttcgattc gatgctgccg ggcatggacg
     9241 cggcgaacgt gccgattccg ctcggcggca cgtccaatca cttcctgcgc tcggcgctgg
     9301 tcgaagtggg tggctgggac ccgtacaacg tcaccgagga tgccgatctc gggctgcggc
     9361 tggccgcgtc cggataccgc gtggacgtga tccagtccac cacgtgggag gaggcgaccg
     9421 cccagatcgg gccgtggatc cgccagcgga cgcgctggat caagggctac atcctcaccg
     9481 ccgccgtgaa tttgcggcgg ccgttgaagt ggttgcgcgg taacggcgtg gccggcggac
     9541 tgaccatgtt cggactgatc cttggcactc cgctggcatt catgatgtac ccgcttgcgc
     9601 tggggttcac gctcgggtcg tggctgctcg ggccggtgtt ccgccttggc ctaccggcgt
     9661 ggctggtgga ctttggcctg ttcaacatgc tggccttcac catcttgatg atcgcagtct
     9721 cggcgctggc cgcgtggcgg cgctacaact ggcggatcgc cgtcttcgcg gtgttcaacc
     9781 ccgtctactg gctgctgcac tcatacgccg cctggcgcgc cgcactgcaa gtgttctggg
     9841 cgccccacct ctgggagaag acaccgcacg gactcaccga ggaatacgac gacggcatca
     9901 tcgagaccgg cggcctgcca cactcggcga gcacctgaac gccgactcag cccagcgcag
     9961 gcgaaaccag ctctggcgac ccgacgaccg ctgcgaccgg acgcaccgcc agccacgcca
    10021 ccagggcgat cgacgcgaac accagcgctc cgaccgcgaa cacagcggag tggccggcca
    10081 ccatcgcgtc cacgccgccg gcctgggagc gtccggcgaa cccgacgtag accgcggtca
    10141 gcacggccag accgatcgag ccgccgagtt ggttcgtggc cgtggacgcg gctgacgcga
    10201 ccccggcatc gtgtggggcc acccgataga gcgcaaggtt gttcaagggg acgacggtga
    10261 agcccatccc gacccccatc agcaccacac cgggcagtac ctgcgtccag tagctgccgt
    10321 ccgcggtcac aaaggtgagc cagaaggtgc ccagcgcgct gatcagcggt ccgaagatca
    10381 actgcggacg cggcccgaac cggtccatca gccgcattgc gaacgggacc gcggccatca
    10441 gcgaaaccgt gaagggcaga gttcccaggc cggccagcag cggtggcagg tgcaacacca
    10501 actgcaggtg aagggcgagg tagaccattg cgcccactcc gaccacgccg agcagggcct
    10561 gcacagagaa tgccgctgcg cgggtcggct cggtgaggat gcgcagcggc agcaacgggt
    10621 gtggcgtccg ccgctcgatc gcgacgaagc ccgccagtag cgccagcccc gcgatgatca
    10681 ggccgagcgt ggccggggag gtccatgact gctcggcgag ggtcagcccg tagaccaggg
    10741 cggcgaagcc gactgaccgg cgatcttgcc cttgtcgagg tagccgccgt agctgttgat
    10801 cgtcaccccg tacttctcgg ctgccgctcg gacggccgcg atctcctcgg ccttcggccg
    10861 cgggtactgg tcgaagacca tcgagccgat cagctcgaac ttggccacgc ccagccggtt
    10921 caggtcggcg aacatctcct cgacgccata gcccggtgcc ggacgcatcc tcgttcgttt
    10981 cgggcagcac ggtgggcccg gcgctgacga tcatggcgct ggccgcccgg acgtccgcgt
    11041 tcattgccga gcagcatgcg gtgggcgcgc tgaccacggt ctagccacaa actgtggccc
    11101 agtgcaccgt ccacggtgca cgcagccaca gcctaaggcc ggacttgcga tttacattcc
    11161 gattgccatc gtttcaaaag tcaactagaa tccagccata agcacatcga aagccgaggc
    11221 catggagatc ctgttcgaca ccgccaactt ggacgccatc gagacgatga cgccgatcta
    11281 cccggtggcg ggggtgacca caaacccgac gatcatcaag gccgagggca ggatcgactt
    11341 ctacccgcat ttccgccgca tccgctcgat catcggacgc gagcgcaccc tgcacgtgca
    11401 ggtgctggcc agggacgcgg acggaatcat tgcggacgcg caccgcctgc tcgaccagat
    11461 cgacgagaac gtgtacgtca agatcccgac caacgagcag ggcatcaagg cgatgaccgc
    11521 gctgaagcgg gagggcgtcc gagtgaccgc gacggcgatc tactccaaga cccagggcat
    11581 cctggcgatc gcggccgggg cggactacat cgcgccctac ttcaaccgca tggagagcct
    11641 cgacatcgac accagttcga cgatcaagtc gctggccaag ttcatcgacc ggcagggtgc
    11701 ggactgcaag atcatggccg ccagcttcaa gaacgtcgcc caggtgagcg cggctttcga
    11761 ggccggcgca cacgcggtca ccgtgcaccc caccctgctc cgcgccgcct tggccacccc
    11821 ggacgtgaac caggccgtcg acgccttcgc cagcgactgg ctcgacacct acgggacaga
    11881 ctcgctgccc tgatcggtag cggacggccc agccacgaga cggcgcccga gccagccatg
    11941 gccctcgacc gccagcacat agggctagcg ctcgacgcgg tcggctggcc ctcggcgagc
    12001 taggccccgc aggcgatccg gaagtcagga gttcagctcg accttggctc gactcgcctt
    12061 gaagccggat ccggtggcgg agttgtggca gctcacgccg gccttggcca tcgtgcagct
    12121 gaagtcgccg gcgaccaggg tcttgccgta gggcaggatc gccagggtct gggtggggtc
    12181 gacactccag ggtgccgact cgccgaagga ctcgtcccac caggcaacgg gagactcgtc
    12241 cgggttttcg gaatcgtaga agaacggaaa cgaatgcggg tcggtgccgc actcccaggc
    12301 cgcgttcgcg gtgccccaga tctccggacc catcacgccg ccctcactgc ccttgcagac
    12361 cgcgcggctg ggctcgaccc cggcgtccca atcctgcccg gtgcagcccg cgccgacagt
    12421 actgatgaag caatggaagt tgccggtcgg cgaggcgaag taattgacca gcatctcccc
    12481 ttcttggcca tctgccggcg caccgagctt tgagacgtcg cgcactttcc cgtccgcact
    12541 tgttgacgga cgcaccgccg tagcgctggg cgaggagccg gcagactcac tcggcgccac
    12601 cgaagtggac gcggggccgg gctgcggcga ggcggcaacg atgctttggg aacgacccgg
    12661 cagcgtggtc acgccccaga tcaccccgac cgacaacacc acggccagca gggccacgcc
    12721 gccggcctgg accatgcgga cgcgtcggcc aacagccttc gcccgtccag ccagaccgcc
    12781 atccggagca gttgccgggg tgtgctcgtt cagggacgag cggatcagat cgtccagatt
    12841 gttcatgatt cgactcctcc caggatttcg cgaagattgg tgagggcgcg ggagagttgg
    12901 ctcttcaccg tcccctgcgt gatgccgagt gcgtcggcga tggcggcgag gtcgtaatcc
    12961 tcgaaatagc gcagcacgac caccgcgcgt tgccgccgtc cgagccggtc gaggccccgc
    13021 cagatcaggt cccggtcgga agtcacgtcc gcgtccgacc tcgaccccgt cacctcgggc
    13081 aggtgctcag tcggcgatac ccgggagccc caccggcgcc aggccgacac gtaggcgcgc
    13141 gccatcgtgg tgcgcgcgta gccgacgacg tgctcatggt cagccatccg attccaggcc
    13201 aggtagacct tgaccagcgt ggtctgcagc aagtcgtccg cgtccgagcc attgccggtg
    13261 agcgcaaggg cgtagcgccg aagtcgcact tgatgcgagg cgacgaacga ctcgaagtcg
    13321 agttcgggcg ccaactccag ggtgggagac ggggcggtca tgtcccttga acgcggcgcc
    13381 gctggcaaag gttgcatcac cgaacccgat ccgcttcgtg gatcagagtc gcgcgatcgt
    13441 cgtgatgtct tcgaggaagt cctcgacggt ctccgccgaa accgtggctc gcgttccggc
    13501 cagcgcctcc agatagtcgg cggtggccag ctcctcacca gcggacgggt cgtcgcccga
    13561 gcgcagcgca cgcgccaacg cttcctggga cgccttgcgc gccgcgtatt cgatgtcggc
    13621 gggcgtgaaa ccctcgctcg ctgcgaccag ggccgcgctg tcgatctggg ccaacactcc
    13681 ggccgggatg tagcgccgcc agatcgccgc cctggcctcg gcgtccggca ggccgatcgg
    13741 aatcacgtag tcgaaccggc catgccgcag gaacgcggca tccagcgcac ggacgaagtt
    13801 cgtcgcgcag atcagcagcc ggccctggtg ctcgcggaag tcggccacca gcttcagcaa
    13861 ctcgttggtg acgccctggg tcggcgacgg cggaatcccg ccgcgacggg acgcgatctc
    13921 ctcgacctcg tcgatgaaca ccaccgcgtg ctccaggtgt ccgatcacct cgaacgtcga
    13981 gcgcagcgcc gcggcgagcc cgccgggcgt gtccgacagg cgcgagggga acaattccac
    14041 gaacggccag tccaaccggg acgcgaccgc ccgcgcaaac gtggtcttgc cggttccggg
    14101 cggcccgaac agcatcaccg cgctcggtgg caccaccccg aagcggtcgg ccagatcagg
    14161 gttggccagc ggcgtgacca gtcgctcctc cagcagttgc ttctcctgcc gcatcccgga
    14221 aacccgcgtc cacaggttgc gcggcaggat gcgtccgccc acttcggcga gcaggtcgag
    14281 ttcgcggcgc tgcaccggca tctggcgttc caggtagcgc aggtggttgc gcacttcgaa
    14341 gccgttggcg gtaagcccgt cgagccgatc cgcgtcctga ggcacgatga tcgagagctt
    14401 gcccaggccg agcggcgcca tccgatgctc cagctcatcg agcaggccgg ccccgacctc
    14461 ggcaccggcg atgtcgtcgg tgagtccgaa gaacacgatc cagccttggg cgtgcgcggc
    14521 ccgtccgacc gcagcgccga ccaactgctc cccctgaaca gcgaccaccg cgtggtcctc
    14581 acgacaggac gcgatgacct cggacaacgc gtacaccggg cgcgagctgc ggtgccccgc
    14641 gtcctcgtac aggcgcacga tgccgtcaag gtcgtcgtcg tggaagtcgc ggatgatcgt
    14701 tcggctcatg gccggattat cgcggtggcc ggcatgagcg cgatagcgca catctcgaat
    14761 tcgccggctg gcccggaaag gtcagcccgc aagctcaagt cgcgtgatcc gggtgagatc
    14821 aacgcgcctt cgggtcttcc agagatcatg ggcatcctgc atggctagcc agctttccgc
    14881 gctacgcccc agcaccttcg acagccggag cgccatttct ggactgactc gactggcgcc
    14941 cgacagcaac ctggtcagcg tcgagggcga gacttccagg cgagcggcga gctggcgcgc
    15001 gctgaccccg aacggtgtca gatacacctc agtaatgaac tcgccggggt gtggcgggtt
    15061 gtgcatcggc ttgagttcgt ccatcagtgg tagtcctcgt aatccaagac ttaggcgttc
    15121 ccttcccgga actcgaacgt caacctccag tttccgttca ctgtgatcga ccagcgcccg
    15181 accatgcgcc ccctgagggg attcagtcgc cagcctggaa tgtccaggtc gtccaccgtc
    15241 tgcgccgtat ccagcgccgc ccgttgcact cgcaaccgtt tgccgtgtgc gggctgcact
    15301 ccctgtgagc atgtggtggc cgtcagatga cttagccgaa gctaggtcag ctggcgggcg
    15361 tgggaaggct cagcgccagc cgagccccgg cgcgacctgg gtgagcaggc tctccagcac
    15421 atgggcgttg tactcgacac ccaactgatt cgggacggtc agcaacagcg tgtccgcggc
    15481 ggcgatcgcc gaatcctcag ccaactcctc gatcaggcga tcgggctcgg cggcatagga
    15541 gcgaccgaac cgggccacac cgccgtcgat gaagccgacc tgatcggact cgtcgcgtcc
    15601 gctaccgaag taggcccggt cgcgatcgtc caccagcggg aagatgctgc gactcaccga
    15661 gacccgcggc gtccaatcgt gcccggacgc ggcccaggtg tcgcggaagc gctggatctg
    15721 ctcggcctgg agttcgtgga acggcacccc ggtgtcctcg gtgagcaggg tggaactcat
    15781 cagattcatc ccctgctggg cggtccactc ggccgtggcg cgggaaccgg caccccacca
    15841 gacgcgctgc cgcagccctg gcgagtgcgg ctcgatccgc agcagtccag gcgggttcgg
    15901 gaacatcgga cgcggattcg gctgcgcgaa cccttcgccg ctgagcacct gcaggaacac
    15961 gtccgcgtgc tggcgggcca tgtcggcgtc cgtcttgccc tcgggcggga cgtagccgaa
    16021 gtagcgatag ccctcgatga cctgctcagg cgatccccgg ctgatgccga gctggagccg
    16081 tccgccggcg atcaggtcgg ccgcaccggc gtcctcagcc agatacagcg ggttctcata
    16141 gcgcatgtcg atcacgccgg tgccgatctc gatccgggac gtgcgcgcac cgatcgcggc
    16201 cagcagcggg aacggcgaac caagctgctg cgcgaagtga tggacgcgga agtaggcacc
    16261 atccgcgccc agctcctcgg cggcgaccgc cagttcgatc gactgcaata gggcgtccgc
    16321 cgcgctacgc accgccgagt gcggcgaatc cgtccagtgn nnnnnnnnnn nnnnnnnnnn
    16381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16441 nnnnnnnnnn nnnnnnnnng tgctggacgc atccgcgcct ggcgcgacgc cgcagcggcg
    16501 tccgttcgcc gaggtgacga ccatcccgga accgtcgttc ccaggcgatt cgaccctgaa
    16561 cgtgaacgtc ttcacgcccg ctccgggacg aactcacgcg aagctgccgg tgctggtttg
    16621 gatccacggc ggcggcttct tcgccggttc gccgtcaagt ccctggtacg acggccgcag
    16681 cttcaatcgg gacggcgtgg tgaccgtggc gttgtcgtac cggctcggtt tcgacggctt
    16741 cggctggatc gcggacgcgc cgctcaatcg cggcctgctc gaccagcttg ctgggctgcg
    16801 ctgggtgcag gacaacatcg cggctttcgg cggcgacccc gatcgggtga ccatcgccgg
    16861 acagtcggcg ggcggctcct cggtgttggc gctgctggca tctccgctag ccaaagggct
    16921 gttcacccgg gtgatcgcgc actctggtgc cgacctggcc atcaccgtcg aacaagcgaa
    16981 gagcatcggc ctacgggccg ccgagctggt gggggtggag cccacgcggg cgggctggtc
    17041 ccagctcagc gaggacgcga ttctcgacgc gcagctgacg ctgatgccca tggacggggc
    17101 gccaccggcg aatccttccg aatacgttgc cggcgtgttg gccggcggcg ctctggggct
    17161 gccgttcatc ccgtccgtcg gtgacgaggt gctgccgttc ggaatcgcgg acgcgctcgc
    17221 tcgcggcatc gggagcgacg tcgacctgat ggcggggacc gtcgcccacg agttcacggt
    17281 cgcggccgcc gcggcgcgcg aggcgtgggc cggactcgac cccgtccgcg cgctggtcga
    17341 cggcggcgtg gcgcccgagg tggcggcgca gtacgtggcc ggccacccag aactggctac
    17401 caccgccgac gtctgcggcc agttggtcac cgacttcagc ttccgcgttc cgacgctggc
    17461 ttgcgcggac gcccacgccg agcgcacctg gctgtacgac ttccgctggc cggaacctgg
    17521 cacggggatg tccacgcact gcatcgacct gccgttcgcc tgggacctgc tggacgccga
    17581 aggtgtccca gccgcgctcg ggccgcagcc gccccaggac ctggcggacg agatgcacgc
    17641 cgcctgggtg cgcttcatcc gggacggcga ccccggctgg cccgagtggg acggcacgaa
    17701 cgcccgagtg ttcggcgcgg acgccgccga cacctaccgt ccagagcagg tactgaccgc
    17761 gtcgctgcca ccgcgcccct gagcgatcag atcccccact agtgggggta ccgcggcccg
    17821 cgtcccgcgt cccgcttcga gagtgcggac gcctgctgct gcgcgcgccc tcaaccgccg
    17881 gccagcaggc cttcaccacc gatcgggagg taaccacctt gtccggattc ctgtccaacc
    17941 tctgggactt cgtctggatg ttcttcacca tcttcgtgtt cttcgcctgg ctgatggcgc
    18001 tgttcagcat catcggcgac ctcttccggg accgtgcgct ctccggctgg gccaaggccg
    18061 tctggctgct gctgctgatc ttcgtgccgt tcctgaccgc gctggtatac ctgatcgccc
    18121 gcgggggcgg catggctgag cgggccgcgt cccaggccaa ggcgcagcag aacgtgaccg
    18181 acgactacat ccgctcggtc gccggcgccg gacccgccca ggaactcacc caggccaaag
    18241 cactgctcga ctccggcgcc ctctccgccg atgagtacgc ggcgctcaag gccaagatcc
    18301 tctcctgacc agctaaggac gcagcatgat ccattacgaa ctgcccacca cgtcagacct
    18361 gcaggctctt ggcgcaacgc gtccggacgc cctgtcgatc tacctggcca ccgagcccac
    18421 tcccgccggc cgtgaactgg cgttcaccag cgccaagagc gcggtcgacc aggccgtccg
    18481 cagcctgcgg gatgccggcc gtagtcacgc cgagcaagaa gccttccgcg cgcggtggga
    18541 ctcagtcgcc gacgacgccg aactctgggg caacctcgcc agttccctgg tgatcttcct
    18601 cgcccccgat ttcgccgacg agtatgtgct gccgaacagt ttcgacgcgc acacccactt
    18661 cggcacgagc ttcgacctgg ggccgctggt acgggccgtc accaccccgc agcgggcctt
    18721 cgcgctcacc ctctccagcg acggctggaa cctgtggcag gccaccgagt cgagccgggc
    18781 cgccgagctg ccgctggtcg gcgagcatgc cgaggacgca gcggacgcga ccaaccggac
    18841 cagcattcgt ggacgccaac tgctgcgccg cctcggtggc gacgagggcc agaaggtgct
    18901 cctcgaccgc tacgcccagg tggtggcgaa ctccgttcgc gaggagctcg gccacttgga
    18961 ccggcacgct gagctgccgc tgttcctgtt cgccaacgag ccgctggctt cgatggtgca
    19021 ggcgcacggc ctgcccggcg aagtggtgct ggtgccgggt gctgccgacg agctgcgccc
    19081 ggaccagatc gacgcggcga tccgcgagcg catcggcgaa ctcaccggca gccggctcag
    19141 ccgtgccgcc gaccggctgg gtgacggctt cgccgccggc cttaccagcg ttgatgtcgc
    19201 ccaggtcgca cgggccgccg ccgcaggcgc ggtcgggacg ctgtactacg acctgaccgc
    19261 cgacgtgcgt ggctcgctcg atcccgagac cggcgcggtc agcttcgccg cggacggcga
    19321 caacctgctc gctgagatcg cgttgctggt gctcgccaac ggcggtgagg tttacgccgt
    19381 acgtccgggc gagatcaagg ccgagatctg gaacggccag ctgctcgccg gtctgcgcct
    19441 cccgctcgtg tgagccagct ggcctagttg tactaaccgg acaggttggt cgagcttgtc
    19501 gagaccacac tcgatctcga caagctcgat cacccgaaaa ggccagccgc aaaacgatcc
    19561 ctgagcttga cgaagggtcg tccccaaccg ccgacgctca accgtaaaac gatccctgag
    19621 cctgacgaag ggtcgtcccc aaccgccgac gctcagccgc tggcgaccag ttcgccggcg
    19681 gccgaccact gcacctgaac cgcttcgtcc tcggtctgca cgaccacgct caacgcggcc
    19741 ggacggttcg ctgggaacgt ccggatcgtc acgcgtccgc tggtcaccgg agcgatcgcc
    19801 gtccggcacg cctcggcgag tcgggcatcg acgagttggt cgggcgcggc cggctccccg
    19861 agcaggacca ccgccacgcc gtctcggcgc aatctgtcca cctcggccat gagttctgga
    19921 tgctgcaggt gcggcgcccg aatccggtcg cggacggcgg cttcggcgcg agccaactcg
    19981 gtccgcaact cggcatcgat gacctccccg ttcgccaacc ggtccagcac cggcaccacc
    20041 aggtcaccga tcgcggcgag ctcagcccgg gagaacgcga ttgcctcagc cgaagccacc
    20101 gcccgctcag ccgaacgctc ggccgagccg cgatgcgccc gctcactacg gacgatccac
    20161 ttcagcacca gccgccacat cacccccgcg gcaacacagc ccagcggcac cccgatcagc
    20221 atcgcgagcg cggggccgtc cggcgactgc ggcagagccc aggccacggc cgcgccgatc
    20281 agcagcccgc tacccaccga acccgccacg acgttgccac gcggaatctg gaacgccatc
    20341 aggtaggtcg cgaagttgag cgcggccagc tcgcccaccg attcagcgga cgcgagcgca
    20401 gcccagacca ccagcagcgc cagcgggtgc agccagagca gccgggcaag caccaacgga
    20461 cgcgcgcccg gcgtagtcaa caggagcgcc ccaaccagtc ccgccggcaa cgcgatggcc
    20521 caggccaagg gctcggcggc cagcgaaccg tccaccaaca cctggagcac gccgcacgcc
    20581 cagacgaaga cgaacgacca tcgcgcggcc gcggtaccca gaccgccggc gccggtgccg
    20641 gctgcggtca agtcggccat gtcagcacca cctccgtccc acgccctggg gccgtatcga
    20701 tccgggcgga gccgccgaga tcagccatcc ggtcaacgat gctccgctgc acgccgagcc
    20761 gctgactgtg ggggtcgggc gtgtagccca ccccgtcgtc gcggacgcag acccggatca
    20821 gggtcgcgga caaggtgatc tccacccgtc gcgaagtgtc ggggccggca tggcgaacgc
    20881 tgttccgcaa cgcctcaccc gccgccaggg cgatggtgcg cacgaccttc gcctggtgct
    20941 ggctgggacg gcactcggtc tcaagcgtga acgcggcatc gatccgccgc aggctcgcgg
    21001 tcagcagcgc gagcgcgtcc gtgctggtca gcgggcggtc gtgctccggc tcggggccgg
    21061 tggagaggct gatcgcttcg atggcgttgc gggcgtcccg tcgcagcggt tgggacgggc
    21121 tgccgtccgc gtgcatcccg gcggagagcg ccgagagcac ctcgtcgtgg acgaagcggg
    21181 ccagcaccgc gtgctgctca agtcgggcag ccgcgcgcac ctggcgctcg tgctgccgct
    21241 gcgcctcccg ctcgccggcg tgcagccgct cgagttgaat gcggacgccg atgaacacgg
    21301 ccacgtagac cacattgccc agctcgttgg gcgtctggat gaggatctcg tcggtcagcc
    21361 ggccgagggt cagcagactg gtcaaggcag gaaccagcga gaaccccaga cccaccgcga
    21421 ccgccacgac cggcttggcg atcagcagca gcaatgtgat cacggcgggt tcgaccgtcc
    21481 acagccatgg ccgggccgcg tccacatcgg gcccgacgaa acccaccagc caggtcgact
    21541 gaaggacgag gtgggccacc ggcaccgtcc gccagatggt gcgcagcgct gaccgaggca
    21601 gcatcatgcc gctcaccgcc agtccgatga tggccgcgat caccagcagc gcaccggcgt
    21661 tccacaaggg cgcgaagtcg gccagccggc cggtctggac gatcgtctgg tcgaggatca
    21721 ggacacagcc gccgagggcg atcagccagc cgagcggacg gtcgatcgcg tcccagcgcg
    21781 cctgctcatc agctgtggtc atggccctgc ggcacgagtc cgtcctcgac cgcgcgccgg
    21841 tagaggtcga ctttggtcag cgccggacgg tcggccgcgg cgtacttggc gcgaatccgg
    21901 cggacgtggt cgtgcacggt ctcgcgggag atgaacagca gcgcggctac gcgttccgcg
    21961 gtctcgccgg aggcgtagag ggtgagcacc tcggcctcac gaggcgagag atgcaccgtg
    22021 gcgaagtcgg ggtcgaagtc gagcgcagcg gcccattctg cggtgaccac cggctcgccg
    22081 cgaagcaggc tgcggatcgc acccacgacc acgtccggtg gctccgattt gcgcaacacc
    22141 ccggtagcgc ccgctcgggt ggcttcgcgg atcaggtccg accggtcgcc ggaagtgtag
    22201 accagcaccg gaatcgcgag cgcggtgagg gcggcgaggt tggcggttgg cgtggaaccg
    22261 tccgcgagcc tgaggtcgag caggacgcag tccagccgct gggtctgggc gatgagttcc
    22321 tggacactcg cggcggccgc gacgacgtgc aggtcgggct gcgcgttgag gatggcaccg
    22381 actccgagca tgatcgcagg atggtcgtcg accatcccga tctgtgcctt cacgcatgcc
    22441 ccccgaccac ccgccggtgg tgcggcccag cctacgtcaa gggacgcggc gccttcgtgc
    22501 cgtcccggtc gcgctcccgc cgcccggtgg cccgttgctc gcctcaacgc ccagcggggc
    22561 gggcctacac caccgctacc gtgccgcgac cggtgaggcg gccgccgatg gctgagccga
    22621 cgaggatgcc gagcatgatc gccaccatgg tggcaccggt cgtcaccagc gttgacagac
    22681 cggtcgagtc gccgtccaga atgctggtga caccgaccag accgagcgag cccggcacca
    22741 gcagccagaa agccggcagg aaactggcga aggccgcggg accgtcctgc tggcgcgaca
    22801 ccacgtaggc caccggtgtc atcgcgaagg caccgatcat gcccgacagc acgccgccga
    22861 acagcacgtc gcccagcacc tgacccccgt aggcgacata gagcaccagc aggatccagc
    22921 cgatcgatcg cggccggcca cactggtgaa tgacgatccc gatgccgaag atcgccaccg
    22981 ccagccatgg cccgagcgga ccgatcgggt tctgtgcctc gaccagctcg acccgcggca
    23041 cgccgaccag ggccgcgccg gacaccagtc cgaccgccaa caggatcagt tgcatgctgc
    23101 cggcagccag ccggccggca ccggccatca tctggccggt ggccaactcg atcacccctg
    23161 tggtcagcag gccgccgggc aacagggtca ccagcggcgc gatcagcgcg gccagcaccc
    23221 cggggtcgag cccgatctgg gtgagggtaa acaccgaagt ggacaccaag aaagcaagac
    23281 cgaccgtgac cagcgcctgg tactggcgtc cgagtggctc ggtgaacagc agcacgacac
    23341 cgaccaccag gcccagcacc gcggccaccg cgacgcctgc ccaggaaccg tccagcagca
    23401 ccgacaatcc cgccgacagc accacgtggg caaccgcgcg ctgggtcgcc gtgtatgggg
    23461 acggcagcgt gcggacgccg gccaccgcag ccttgatcgc cccgggctcg gacggcaggg
    23521 tgcgcgcggc ccggatcacg tcgtcgagga cgtcgatctg gttgagacgg agctcctggt
    23581 ggccggccga gaccgccccc gtccgcactg cgccgaaccg gcgggtcgac acgaacaagg
    23641 ccgtcggcaa gaccacgatc tcagtggcgg gctggccgtt cacgcgggcg atgtcggtca
    23701 gcgcggtccg caccccggtc accgagtagc cggcgtcgat catcgcctcg ccggccgcct
    23761 ccaggtcggc gagtccggcc accatcgcgc tgtcggacgg gccgatggac acgtccggct
    23821 gcggtgcgat cagctggtgc tccggtgggt ccggacgccg cgccagaagc aacaccagga
    23881 cggcggtgct gatgagtacg accagcgcga tgatccagga cagctccgcg ttggcgagct
    23941 gccaggccgg cggatgcggc tccaggacgc cgttgcccgg gatgtcccgg ccagcttcgg
    24001 ttggtccggc tgtcgcggcc ggaaagtcgc ccgcggtcgg gctcggcgcg gacgtcggac
    24061 cggcggtcgg cgacggttcg ctcggctcag tcggggacgg ggtcggttcc gggacggtct
    24121 cggtcggctt gggctcagac gcttcgggct cgtccggctc ggtcggctcg gcggacgcgc
    24181 tgatcggcgg cgggtcgctc ggcgtcggct cgaccgcgaa tgccggggca aggccaaccc
    24241 cgccaagccc aagcacagca agcacagcgg acgcgagccc tgcgcgccag ttcgggtgcg
    24301 gacgacctcg gctggccggg accccgctcg atcgtgcgtc gagagatccc ggccgtggtt
    24361 cacggctggt cgagcttggc ctgccctgag cctgtcgaag ggtcgagacc acgtcctgcc
    24421 cgccgaacgg ctccagagat cccggcctgc gccgggatga cgtttggccg aaccgtgcgt
    24481 ccgctgccat cagacccgcc cggcgggcga gcgagccggc acagaactga acaggtccag
    24541 gccgagctct gcggccagga agcgggccgc gagctggccg cggacgctgt tgccgtccga
    24601 atccagcaag ggcgaaaacg cgcccagcgc acccttgccc ggcgacaccg cgaccaggcc
    24661 gccgctgatg ccgctcttgc ccgggaagcc gacgtcgagc agccagtcgc ctgagccttc
    24721 atacagcccg gcgacgctca tcactgcgag ggtggcccgc gccaccgcag cactgaccac
    24781 ccgctcgccg gtgagcgggt tcaccccgcc gtcggcaagg gtcgcaccca tcgtggccag
    24841 gtcgaccgcg gtcaccgcga ggcagcactg ctgggtgtac aggtccaacg cgaccagcgg
    24901 gtcaccgacc aacgcgcccg cttcctgcag cagcatcgcc agcgcccggt tgcggaagtt
    24961 ggtcgcacga gcggacgcga gcgtgtccgc gtccacctgc aaccggcggc cggcgaaccg
    25021 cgacagcccg tccaccaacc agtcccagcg agcggacggg tcgccgcccg gcaccagact
    25081 ggtggtggcg atggcaccgg cgttcaccat gggattggtg cgaccccgat cagcgcgctc
    25141 cacggccgcg atcgagttga acggcagtcc cgtcgggttc acgcccacca aaccgcgcac
    25201 ttccgcgatc ccgatctcct cgctcaccag ggcgaagacg aacggcttgg cgatgctcat
    25261 cacggtgaac gccacttcgg cgtcgccggc gaaccgcgtc aggccgtcca cgccgaccag
    25321 cgccagcccg aacagatccg gatcggcctc ggccagcacc gggtacaccc gcgacagttg
    25381 cccgtccgag gtgtcgcgaa actgcgccaa ggactggtcg agcagctcgc cgaccacccc
    25441 gtccagcggc agcctgccag tggagacgta ccgctccgcc ggctccgcgg acgggtggtc
    25501 aggcaggtac atcagccggc ggaaggcgtg aaggcgcagc tcagttcgag cagcagccgg
    25561 gtgccgaagg ccgtggtgcc gcggttgatc cagcggtgcg cacgcgacga ctgggccgta
    25621 ccggcaatgt cgatgtgcgc ccacggcacc cccgcgacga attcctcaag gaacaggcca
    25681 gccgtgatcg aaccggcgtt cggaccgccg aggttcttca ggtcggcgat gtcggaatcc
    25741 aactcggcgc ggtagctgcg gatcagcggc agccgccaga ccggctcgtc gactgcgtcc
    25801 ccggccgcgc tcacctgggc ggccagcgcg tcgtcgttgc tcagcaggcc ggcgacttcc
    25861 tcgccgagcg tgcgcaggca tgctccggtc agggtggcga tgtcgacgat ggcgtcaacg
    25921 ccctcctcga ccgcgaggac gagtgcgtcg gccatcacca ggcggccctc ggcgtccgtg
    25981 ttcagcacct cgacggtctt gccgccacgg atcgtgagca cgtcgcccaa ctgcatcgcc
    26041 gagcccgagg gcatgttgtc ggtgcacatc aggtagccgg tgacggtgtt gcgacagccg
    26101 agctcagcca gcgccgacat ggccgcgagg atgttcccgg cgccggtcat gtcgttcttc
    26161 atctgcgagt gcgacaggtc ggccggcttc agcgcgatac cgccggagtc gtacatgatg
    26221 cccttgccga ccagggcaag gtgcccggtc gactccccgg acggcgtgta ccgcagcacc
    26281 accatccgcg gctcctcgat cgagcccttg ttgacgccga gcaggccgcc gcagccgagc
    26341 tcgatcagcg ccctgcggtc gaacaactcc accttcagcc cggactccgc cccgatctgg
    26401 acggccaact cggccagccg ggacgcggtc agcagcccgg ccgggcagga cgcgaagtcg
    26461 cgtccgagca ttccggcacg ggcggacgcg agcccgcggg tcgcaccagc gcggacgtcg
    26521 tcgacggcgc cctcgggagc ttcgagaacc agcgactcca ccgccaccgg accagcagcc
    26581 accgacttga acgagaactg gtagcgggcc agcagcacgc cctcgacgat ggcggcagcg
    26641 gcgtccgcca ccggcacctg cgcgatggcc ggcacccgaa cagcgagggc ggcgtcctgg
    26701 gggaccgcgc gggcgaaggc agccgccgcg tcgcgcagcc gagcaacagt gagttgctcc
    26761 gggtcgccga cgccgaccgc gaccagcacc ggctggttca ccgaggggaa cggaaccgcc
    26821 ttgcccacgt ccggggtgaa gcctgcccgg gccagcgcag ccaggtcgac accacctagg
    26881 atcgccggcg tcggctgtcc ggcgagcacc gggacggcga gtgcatccgc ggtcatagtg
    26941 ctgcctgctg ccaccacatc aaggtggacc gaacggttgc gcgaggggat cggatcgagg
    27001 tcgagcgttt tcatgggggc cttcttcgga gtgtggggat tagggatcag atattcaccg
    27061 gggcgcccgg accccaaggg atgccgagca ggaaccaagc gacgaacagc actacccagg
    27121 ccagtgtgat ccacagcgtg tagggcacca tcaaggcgat gatcgtgccg atgccggcgt
    27181 ccttcttgta gcgctgcgcg acggtcacca tgaacggcag gtagaccatc agcggggtga
    27241 gcacgttgag cggcgagtcg gccacgcggt aggccgcgag cagggtctgc ggggccacgt
    27301 tgagcgaggc gaagatcggg atgaacaccg gcgcgaagat cacccacttc ggcaccagcc
    27361 cgggcaggat caggtcgagg acgaggatca cgacgatgaa gcccagcagc agcacgatcg
    27421 cgggcacgtt ggcctgctgc agcagttcgg cagccgagat ggccgccacg gtgggcaggt
    27481 tcgaccagtt gaacagggcg atgaactggg cgatcatcag gaacatcagc agcagtccgc
    27541 cgaggctgcc gaacgtcttg gcgatcgcgg tcaccgcctc gttgccgccc tggagcgtgc
    27601 cggcgccctt gccgtagccc aggccgcaga ccatgaaggc cagcgagatc acgaacacca
    27661 gcgaagccat gaacggcgtg ttgccgatga tgttgccggt ctccggatcg cgcagcggcg
    27721 cgcccgggat tgcggtcaac agcgcgatga tcaccgcgaa gccgagcagg taccagccgg
    27781 agtacttcag gcccttcgcc tcggccgcct catcgatctg ctcctcgtcc acgccttggt
    27841 agtcggcgtg gtcgtagacc ccgagccgct tcatcgtcac ctt
//
LOCUS       DAOPRQ010000034        27540 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_1391_length_27540_cov_12.301328, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000034 DAOPRQ010000000
VERSION     DAOPRQ010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 27540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27540
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1391_length_27540_cov_12.301328"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            714..1661
                     /locus_tag="PKJ61_06180"
     CDS             714..1661
                     /locus_tag="PKJ61_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13446.1"
                     /translation="MADLADAASAADEVPLKKTKGGFEVANDDGAEITVTKDGATTLS
                     APGVPEIGISVAGDADASKLVDGVLVQTEVAPSTDVVTRATDNGVQLIAVLADADAPD
                     EIEFPLELPRGGELVELADGALAVMAPVTRVLADPGEWERVNREVESILGANYDVSVD
                     PTPAQEKALASIPPVSTHEEVTVEQVAVVHAPWAVDANGRAVDTHYRVDGNTLIQVVD
                     AGDDAAFPITLDPGVDWWKVTKCVAAIAWAVGSTIFAVAKIAKIRKYIEALGGITQTA
                     KLVMGATTKAEKAIAIGKGLLTFAAIVFGIDQIYENCRP"
     gene            1658..2017
                     /locus_tag="PKJ61_06185"
     CDS             1658..2017
                     /locus_tag="PKJ61_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13447.1"
                     /translation="MIPVQELVWVVSAFVFAGGVIWGVFRGRGRPDVVDSDPADRTVH
                     RDQEYGSDLAFVGFSLAAFVAVYIVRAWGSWDWSVPMIALVGLAGARLADVLRSAKAA
                     QWVPWVFVAVAAGLLAI"
     gene            2294..2857
                     /locus_tag="PKJ61_06190"
     CDS             2294..2857
                     /locus_tag="PKJ61_06190"
                     /inference="COORDINATES: protein motif:HMM:NF012573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HOC13448.1"
                     /translation="MFAGRIRTSLTALAVIGAGAGLALVAPTAATAVPLPMATATCNG
                     LAATIVGTEGSDDIEGTAGRDVIVTFGGNDDIDGNGGNDVICAGSGYDEVDGGEGNDW
                     ISGGSGGDTLEGSWGADKIYGNSGNDHIEGESGKDKLYGGSGHDLLEGGSGADKLSGG
                     SGKDTTVARYASDRTEDRWENRYGYDD"
     gene            3154..4095
                     /locus_tag="PKJ61_06195"
     CDS             3154..4095
                     /locus_tag="PKJ61_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016668194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOC13449.1"
                     /translation="MIEPTLMEAPPPGIEAMGLARAFGDVRAVDGITFTAPAGAVTAL
                     IGPNGSGKTTLLLLLSGLLKPDAGKAIIAGHDVESENLSARSHVGWMPDAFGTWDALT
                     CTEILTTFAHAYGIPRAGTLGLVDDLLRRVHLTEFAHRPARVLSRGQKQRLGLARALV
                     HDPQVLLLDEPASGLDPRSRIELRDLLRDLAREGKTVLVSSHVLSELEELYDHAVFLS
                     RGRTVDASAAGASGSVQRGWRLDAIDPVGLRTFLDDAGIPWTGQPNGEVVVQLSGYDS
                     ARELVRAAVGADVSLHTISPVSGRLEETYLSLNEERV"
     gene            4092..5327
                     /locus_tag="PKJ61_06200"
     CDS             4092..5327
                     /locus_tag="PKJ61_06200"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOC13450.1"
                     /translation="MNTWTLSFGGLRTVIELELKQRIRSKRWIWALAGWFVLLGAVTG
                     LIIWAAHEIYSWDNNNVQAGPMAFGVITFFVLGMGLLIAPTFSAMSINGDRAGGTLAT
                     LQATRLSAFEIAAGKLLAAWLTAAVFLVVALPFIVWSMVLGNISVWQVLVVFGVVLAE
                     VAVVCAIGLGWSAVTSRPAGSAVMTYLSIAFLTVISTMVMGLLIPMVTDEQPVRVWGL
                     TGQVQDDYNRALDKYWAANPDGDGTGMPAAPIGKCTWFVENHTITHADRVWWITVVNP
                     FVIVADAAPLPPGAAKNLSQYQSLGSDPLATIRWGVRQLSMPPQLEFDQCSQYYDGMP
                     GYTVVYDDQGNAIVTTTKGGATVNVESPVKRRVVNVETPIWPWGLAANILIGGVFFWI
                     AVQRLRIPYAMLPKGTRVA"
     gene            complement(5339..6031)
                     /locus_tag="PKJ61_06205"
     CDS             complement(5339..6031)
                     /locus_tag="PKJ61_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020579461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOC13451.1"
                     /translation="MPEPATEPIRVVLVDDQHLVRAGLAMVINAQTDMTVAGQASDGL
                     EALDLLADCAADVVLMDVRMPHLDGLEATRRLIDAADLAGHEPPRVVMLTTYDLDEYL
                     LAAIKAGASGFLLKNAPPDDLLAAIRTVRDGDAVVAPSATRRLLAHVATGVGAPTLEP
                     DHRVECLTDREREVLELIARGLSNAEISAELFLSETTVKTHVRHILTKLEVRDRVQAV
                     VVAYESGLAGSR"
     gene            complement(6024..6839)
                     /locus_tag="PKJ61_06210"
     CDS             complement(6024..6839)
                     /locus_tag="PKJ61_06210"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOC13452.1"
                     /translation="MSPLEVVLLVAAVALAVGLAALFVVHQRTRTELREALAARHRAV
                     NAQKQATELATAAERMRILREMHDVIAHSLAIMIAQADGGSYVVADSAAAKRALVTIA
                     ETGRVALADTRRILGMLRHGEEAELTPTPTQASLDELIEQARQAGLQVSLIRVGQPRG
                     MPSGSRLALYRICQEALTNVIKHAGEGAKVIVAVSWVDDEVTLTVTDRDGEGTRTLDP
                     DAEEPLSGLGLGLIGMRERAELIGATFDAGETEDGFRVRTIIPLAASLEETHA"
     gene            complement(6844..7950)
                     /locus_tag="PKJ61_06215"
     CDS             complement(6844..7950)
                     /locus_tag="PKJ61_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016455424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GuaB3 family IMP dehydrogenase-related protein"
                     /protein_id="HOC13453.1"
                     /translation="MIEIGRSKRALPVFGLDDIAIVPTRRTRGIEEVNLSWKIDALNF
                     DFPMVAAPMDSVMSPATAIAMGRLGGLGVLNLEGLWTRYADPEPLLTELADIDDQLAA
                     TRRMQELYAEPIKAELITGRMAEIREAGVPVAGSLSPQTCAEFASVVEKAGVDFFVIR
                     GTTVSAEHVSTVCEPLNLKKFIYELDVPVIVGGCATYQSALHLMRTGAAGVLVGFGGG
                     ATKRTRNVLGVEVPMASALADVAEARRDYLDESGGRYVHVIADGALGKSGDIAKAIAC
                     GADAVMVGSPLARATEAPGKGYHWGPEAWHATLPRGERVKFGAVGTLEEILLGPSHVA
                     DGTMNLVGALRRALATTGYSDVKEFQRVEVVVHS"
     gene            complement(7966..9459)
                     /gene="guaB"
                     /locus_tag="PKJ61_06220"
     CDS             complement(7966..9459)
                     /gene="guaB"
                     /locus_tag="PKJ61_06220"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014847133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOC13454.1"
                     /translation="MPGPFAALGLTFDDVLLQPNESDVIPSAVDTTTWVTKRIQLRVP
                     LLSSAMDTVTESRLAIAMAREGGMGILHRNMSAEDQAGQVDRVKRSEAGMIDQPITTT
                     LDATIGEVDEMCGHFRISGVPVVDSDLKLLGIVTNRDMRFEDDPNRPVGEVMTPMPLV
                     TGWPGISSEEALQLLAKHKIEKLPLVDADGRLRGLITLKDYVKSDKYPMASKDPQGRL
                     RVGAAIGFFGDAWERAMMLVDAGVDAIVVDTAHGHSQGVLEMIRRLKAEPAAAEVDII
                     GGNIATYAGALALVEAGADGVKVGVGPGSICTTRVVAGVGVPQVTAIHEAAAACRPAG
                     VPVIGDGGLQYSGDIAKALVAGANTVMLGSLLAGCEESPGELVFINGKQYKSYRGMGS
                     LGAMAARGRKMSYSKDRYFQGDVASDDKLIPEGIEGQVAYRGPLSAVAYQLVGGLRQS
                     MFYTGASTIDELHTKGRFVRITAAGLRESHPHDIQMTIEAPNYTSQS"
     gene            complement(9559..10437)
                     /locus_tag="PKJ61_06225"
     CDS             complement(9559..10437)
                     /locus_tag="PKJ61_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002524676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HOC13455.1"
                     /translation="MRVAIGGSTGLIGSALSRHLVGAGHEVVRLVRGEISASDQRRWD
                     PDAGRIDSPGLDDVDAVVNLAGASIAGGRWTKDRKTEIRRSRITSTLTIVTSLSPGGR
                     CQRLLNGSAIGYYGDTGTEIVDETMPVGRGFLAGVVADWEAAAAHSPVSTALLRTGNV
                     LARHGGYLAAQWPAFALGLGGRVGSGRQFVSWISLADHVRATAFLLDSELTGPVNLVA
                     PEPVSNAEFTRVFGSHVHRPTPLPLPLGAVGLVFGREFVKELLLPGQRVRPAHLLDAG
                     FEFEHKTVRQAFAALS"
     gene            complement(10491..11852)
                     /gene="mptB"
                     /locus_tag="PKJ61_06230"
     CDS             complement(10491..11852)
                     /gene="mptB"
                     /locus_tag="PKJ61_06230"
                     /inference="COORDINATES: protein motif:HMM:NF038066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol phosphomannose-dependent alpha 1,6
                     mannosyltransferase MptB"
                     /protein_id="HOC13456.1"
                     /translation="MPIPIATALRSQGVWVGTLGSFAVAWGSCHPEFSPSAYGWPSEL
                     INAIGESAPQPVNRILIVLGVIALTWAWWHIRPTRDRAPVHAGTAMLLWSLPLLLVPP
                     VLSPDAVLYADLGWTLHAGHNPYEVGLATSGGPFAAWVDPLWAGSGVAYPPLSLHLNQ
                     LIVTATGLHPYWSVIAMRIPAVVAVVAMVVLVPRIAKLLVRPARGAVWLGVLNPLLLL
                     HFIGAAHNDAPMVALSLAAVWVVCRWPKWWASFLVGPAVVALAMAFKQQGGLTVIAVA
                     GLPVAAALVAAPMARRLWLLGWRTALATAVTLVVFAGVSLASGLGFGWVEWLDLMGLA
                     GTPAPLALLSKGGALIWQAAGGSYTGFLIVAGRVGTAILLAVLVWLGVRFANRPLALV
                     AWASLVIAVLGQALHPWYLPWSLALLGLIPLNRRQRYWVFGFAIAFCIWNALQTVIWH
                     GQV"
     gene            11931..13703
                     /locus_tag="PKJ61_06235"
     CDS             11931..13703
                     /locus_tag="PKJ61_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015583662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOC13457.1"
                     /translation="MEFSGMGAVVTPEGVGFRVWAPHASAVAVVGEFNDWTSDANQLA
                     GEGNGNWAGFVPDAKAGQEYKFQITNGEQVLTKVDPYAAHVTNSVGNGVIYDHDAFDW
                     AGDEFTPPTFNELVIYELHAGTFASQADGEVSDLDEMAEKLGHIVKLGANAIQLMPVA
                     EFAGDYSWGYNPAHIFAVESAYGGPDALKRLVKQAHALGLAVIQDVVYNHFGPSDLNL
                     WQFDGWSEDDKGGIYFFNDWRSTTPWGDTRPDYGRGEVRQFIHDNAFAWLERFHMDGL
                     RYDMTPYMRDVDGSGFDIPEGWDLCRWINTDIRSKYPEKILIAEDLHSNPAVSSTGPE
                     GAAFHAQWDAQFVHPVREAITVAEDQYRSTDEVLGAVGYSYGDAFARVIYTESHDEVA
                     NGKARVPEEIDADDPTGYFAKKRSTLGAGLVLTSPGIPMLFQGQEFLEGGWFRDNVAL
                     DWDRSDDFHGIVKLYRDLIRLRLNRDSLSAGLTGSGLRVIHNNDAENVIAFQRWREHG
                     VGDDVVVIANLSNQTRTDYRIGLPAAGKWQLLFDGDSAMYSEDFGEHAARDLVASAEA
                     YDGLDASAEISIAPYTLLIYGWRG"
     gene            complement(13756..14298)
                     /locus_tag="PKJ61_06240"
     CDS             complement(13756..14298)
                     /locus_tag="PKJ61_06240"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOC13458.1"
                     /translation="MWPASQPRLVGTACALRPLTDRDVSNLVAACRDPEIARFTRVPV
                     PYTEADARSFVSLAGHAWQSREGSWFAIADAADELIGACSVMDVNQSGSAAEIGYWVA
                     PAARGRGAARTALGLITAWAHRELGLDRLHLQIEDANRASVVVAEAAGYRPLDEFSEW
                     ELKGAPRRFRHYEHLLVGGV"
     gene            14522..15856
                     /locus_tag="PKJ61_06245"
     CDS             14522..15856
                     /locus_tag="PKJ61_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020390895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOC13459.1"
                     /translation="MRAPNQQNREILALAIPAFLALVAEPLFLLADTAIVGHLGTAQL
                     AGLGVASAILLTAANLFVFLAYGTTSVVARQVGGGARGAALSSGVDGVWLAIVLGLVV
                     AVVVGGLAEPLCAAFGATPAVLAEATIYLRISAIGLPAMLVALAATGALRGLQDTRTP
                     LIAAVAGFGANLVLNLWFVLGLGWGIAGSAWGTVIAQTAMAVALVAVLIRLAGREHAT
                     LRPHPHRVLAAARTGVPLLIRTLALRAVILVTTWVAAGLGDVALAAHQVAATIWTFQA
                     FALDALAIAAQALTGKGLGAGDRAAVRAATTTMTRWGLWGGVVLGGLLVLASGVLPPL
                     FTADAAVQAALSWALIVVGVGGPLSGLVFVVDGVLIGAGDGRWLASAMLGTLALYLPL
                     ALLVRWQAPALIGLGAPVGVAVLWVAFLAFMAIRWGFLRHRVRSDVWLVTAS"
     gene            complement(15969..16253)
                     /locus_tag="PKJ61_06250"
     CDS             complement(15969..16253)
                     /locus_tag="PKJ61_06250"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002516071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HOC13460.1"
                     /translation="MQSDPELERLRGSIDNLDSAIICMLAERFKITQRVGVLKKERGL
                     APADPEREVQQIARLRALAEQANLDPEFAEKWLEFVVAEVVRHHEAIAAE"
     gene            complement(16428..17360)
                     /gene="cysK"
                     /locus_tag="PKJ61_06255"
     CDS             complement(16428..17360)
                     /gene="cysK"
                     /locus_tag="PKJ61_06255"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HOC13461.1"
                     /translation="MAYFEDATKLIGRTPLVKINRLYGDSQAIVLAKLEFYNPANSVK
                     DRIGVAIIDAAEASGELKPGGTIVEGTSGNTGIALAFVGAARGYKVILTMPETMSKER
                     RALLRAFGAELVLTPGSEGMRGAVNRAEQIAAETPGAILAKQFANPANVDIHRRTTAE
                     EIWTDTEGAVDYIVAGVGTGGTISGVGQVLKERKPEVKIVAVEPAESPLLSGGQPGPH
                     KIQGIGANFIPEILDRSVIDEVLPQNAETAVEWARRAAKEEGLLVGISSGAALAAAGE
                     LAARPENAGKTIVVIIPSFGERYLSTILYADLLD"
     gene            complement(17413..>18666)
                     /locus_tag="PKJ61_06260"
     CDS             complement(17413..>18666)
                     /locus_tag="PKJ61_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HOC13462.1"
                     /translation="HAGQEEADPATNSRAVPIYQTTSYVFDDTEHAANLFALKVPGNI
                     YTRIMNPTQSVFETRVNALEGGVGALATASGSAAVTYAVLNLTYAGDNIVALSTLYGG
                     TYALFAHTLPQFGIEVRFVDPGKPEDLAQHVDAKTKLVFGETIGNPAINVIDLDAWSE
                     AAHALGLPFIVDNTVPTPLLARVFDHGADIAVHAATKYIGGHGTSIGGVIVDSGKFDW
                     AAHADRFPGLTAADPAYHGVVWTDAAGPAAYIIRARTVLLRNTGAATTPFNSWLFLQG
                     LETLHLRIERHSTNALAVAQYLENHDQVAWVNYPGLESSPSKAIADRVFTGRGYGGIL
                     AFGLKAGREAGAKFIEALQLFSHLANIGDAKSLAIHNATTTHSQLTEDELVAAGVAPE
                     MVRLSVGIEHIDDLIADLDQALAQL"
     assembly_gap    18667..18766
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <18767..20245
                     /gene="guaA"
                     /locus_tag="PKJ61_06265"
     CDS             <18767..20245
                     /gene="guaA"
                     /locus_tag="PKJ61_06265"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002519617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HOC13463.1"
                     /translation="REAQVYSEIVPHTMPVAELLARRPAAVVLSGGPSSVYEAGAPQL
                     DAALLAAGVPVFGICYGFMAMAQALGGTVARTGQREYGRTAVSVLEPGRLLAGLPETL
                     TSWMSHGDEVTAAPSGFIVSARSPRATVAAFEDAERRLAGVQWHPEVLHSEHGQKVLE
                     HFLVDIAGCRQTWTSANIAEEQVERVRAEVGDARVLCALSGGVDSAVAAAIVQQAIGD
                     QLTCVFVDHGLLRTGEAERVERDFVASTGVHLVVANEQERFLSALAGVSDPETKRKII
                     GREFIRTFEDEARKLNAEGPIDYLVQGTLYPDVVESGGGEGAANIKSHHNVGGLPDDL
                     QFKLIEPLRTLFKDEVRQVGRQLGLPEHIVDRHPFPGPGLGIRIVGAVDAERLAILRA
                     ADAIVREETTAAGLDGEIWQFPVVLLADVRSVGVQGDGRTYGHPIVLRPVSSEDAMTA
                     DWSRLPYELLEKISTRITNEVPEVNRVVLDVTSKPPGTIEWE"
     gene            complement(20264..20527)
                     /locus_tag="PKJ61_06270"
     CDS             complement(20264..20527)
                     /locus_tag="PKJ61_06270"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HOC13464.1"
                     /translation="MKQKLTRSSSDKFIAGVCGGLARSFGWEGSIVRILAVVAALIFP
                     WGVPIAYGVAWLVLPLEDGGRTGLDDLKGTFTSNSNNPPTDYR"
     gene            complement(20549..20719)
                     /locus_tag="PKJ61_06275"
     CDS             complement(20549..20719)
                     /locus_tag="PKJ61_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13465.1"
                     /translation="MTDRSAFAFGVIAMAIAGLALWLHYGFVDWRLVGVIAPVVLVII
                     GAGMLLLSRKSN"
     gene            complement(20716..21918)
                     /locus_tag="PKJ61_06280"
     CDS             complement(20716..21918)
                     /locus_tag="PKJ61_06280"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HOC13466.1"
                     /translation="MPDNLPVLSRPRNGAMIGGVCAGLARRWHVDPNLLRIAVAVLVL
                     FGGLGLAAYGAGVLLMPRDGQTEMPVRRWLPFTRDWPTAGVVAATIGALVLLTGIPTN
                     GIGFGPVAVIFAVWYFGFRNHGTRTNAPTPEPTPFERQAEAWRDRLAEQQTPGYAPAS
                     GLLSAASLPAPTLPTSQQAPQWQQPYTNPADLAVRDNLPPAMVAARQRRRRFWLLAAL
                     LSGVGVALVTVLGVVFGLPLGPLPYFAAILAALGMTLVASSKVGRPRGMLFVTIVTAL
                     LTAALMSPVPASVGETAQVYRSVDELPRQIDLGAGEIALDLSAIRLDSGRAMDIKLGA
                     GEVDIILPADGNTLVSYHIGAGTFDNSVHSNDGVDVKGSQTFGSGTGTPVLQLNIDVG
                     MGEVTVRT"
     gene            22265..23278
                     /locus_tag="PKJ61_06285"
     CDS             22265..23278
                     /locus_tag="PKJ61_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020388516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOC13467.1"
                     /translation="MVKTLNADRSAPTLEDVARVAGVSRATVSRVVNGGNLVAKDTTE
                     AVRRAIAELAYQPNRAARSLVTRRTGVIAVVVPETDDRVFTDPFFPQAYHGALQAFQG
                     SDVRVVLAMAQPGDGPAGMVRYLESGHVDGAIIVSHHGPELGRALASSWQPVVFVGDP
                     EAPGVHYLDLDNMGAAATATQHLIDRGSKRIGAITGPLDMNSGRRRLKGFQQAMKAAG
                     RDDTLWVESDYTRNGGRAVLELLERAPDLDGVFAANDLMAATALNVLLETGRRVPEDV
                     RVVGFDDSEAALQTNPQLTTMTNPAAEHTRMAAEMLISILAGENPQPMTKLPSTLVVR
                     GSS"
     gene            23275..24519
                     /locus_tag="PKJ61_06290"
     CDS             23275..24519
                     /locus_tag="PKJ61_06290"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HOC13468.1"
                     /translation="MSEEVVHPRSTRPLAEAWVGGVCAGLADHLGWSVLVLRVLFVVT
                     AAFGLVGVALYLVLWLVMPQEVEQRDAPGLEAGARKGLRPRTTKMLTAVDLGVASALA
                     LLGVGLAWLAQVSGYGPPNWLLTAGALGALGLGIIWWQADHVSTRELRTGTGWQRLVA
                     PLVAHWTSIVGIVAGLVALGGSIWVVLLAQARLSEAARTILLLGLAVTAVVLAALPWI
                     LHVRRALAQARDDALVADARADMAAHLHDSVLQTLALIQRKASDPKAVTALARRQERE
                     LRQWLYADSSGRGTLVEAISGEIIDVEDRHGVDLELVSVGDTELTPELTALVKAAREA
                     MVNAAKHSGATRVDVYVEVDPDVVSVYVRDRGTGFDLAHMDTDRMGVRGSIVERVKRA
                     GGRAIIRTAPDEGTEVRLELPR"
     gene            24516..>24884
                     /locus_tag="PKJ61_06295"
     CDS             24516..>24884
                     /locus_tag="PKJ61_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015071154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOC13469.1"
                     /translation="MTETEPEAVPTGPWRVVIVDDHAMFRSGVKHDIGARIELVGEGE
                     DVPTAVRAILATLPDVVLLDVHLPGGGGAEVIKQVTATQPGVKFLALSVSDAAEDVIG
                     VIRAGARGYVTKSISAEELVA"
     assembly_gap    24885..24984
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <24985..25161
                     /locus_tag="PKJ61_06300"
     CDS             <24985..25161
                     /locus_tag="PKJ61_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HOC13470.1"
                     /translation="REREVLRLIARGYAYKEVAKELFISIKTVETHVSSVLRKLQLSN
                     RHQLTRWATDRKLV"
     gene            25310..>27540
                     /gene="pcrA"
                     /locus_tag="PKJ61_06305"
     CDS             25310..>27540
                     /gene="pcrA"
                     /locus_tag="PKJ61_06305"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016669473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HOC13471.1"
                     /translation="MSEDELLANLNPPQREAVVHAGGPVLVVAGAGSGKTRVLTRRIA
                     HLVSQRNVHPGSILAITFTNKAAAEMRHRVVDLVGNRAKLMWVSTFHSACVRILRSDI
                     GRFGMSRTFSIYDDTDAKRLMTLVLRDQELDPKRYPVRAVMNWVSNQKNELVDFESAR
                     TRAVEGTAEHHYAEAYAEYQRRLKAANALDFDDLIMTTVHLLQAFGDLREQYRRRFRH
                     VLVDEYQDTNHAQYALVRELCAALPEGGEGPSIDPPELMVVGDSDQSIYAFRGATIRN
                     ILDFEADFPGATTIVLEQNYRSTQNILSAANAVITRNPGRPEKRLWSETGDGELLVGY
                     VADTEHDEAQFVAEEIDRLTDAGTSKPGDVAVFYRTNAQSRAFEEVFIRVGMPYRVVG
                     GVRFYERKEIRDAISYLRAIANPDDDVSVRRILNVPRRGIGDRAEAMVEAFAAAEQIS
                     FGAALDRAAEAPGLASRSLSQIQGFAAMLAGFRGKVAAGAPADEILAGVLKESGYLAE
                     LTASTDPQDESRVENLVELVSVAQEFVAAAHAVDVPDDESELAAGAPEPDDSLPAFLE
                     RIALVADSDQIPDNDPDATGVVTLMTLHTAKGLEFNTVFLTGFEDGIFPHQRAMTDAK
                     ELEEERRLAYVGITRARQRLYLTRASVRTQWGAPQYNPASRFTDEIPGHLVDWRRLGY
                     ARTGFASASSARTEQAWRSTVGFGAKPAIKTVPSVAIGDRVLHTTFGMGTVIATSGIG
                     DTA"
BASE COUNT         4341 a         9036 c         9527 g         4436 t
ORIGIN      
        1 ggtcggcgct ggccaacgcc gcgtccatcg cgtccctgct gctcaccacc gagacctcgg
       61 tggtcgagaa gccggccaag gacgaggctg agcacagcca cggtcacagc cactgaccac
      121 cggttggtcg agcttgtcgg ttggtcgagc ttgtcgagac caccgctgag ccgctgctga
      181 cagaggggcc ccacccggtt tcgggtgggg cccttcgtgc gtccgcgatc ctcctgccgg
      241 gctagtccct ctcagattcg gaataaattg ggggcggatg taatcttcgg caacgccgat
      301 tctggaccga acttcggtgg cacatatcta cgcagccttg tcgttgcaga accaaccgat
      361 ggaagccgta cgaactcgta caccgaaacg atgatttggg ccttgaccca cgcccgaggg
      421 cactgggacg gtatcagctg gccagacggc ccttgctgcg gaagcagccc tacccccgaa
      481 gtgcacaagg agacgagatg ggattgccca cctcgatcag gcaggccctg gtcggagtta
      541 tgacggtggc cttcacggtt gccagttgcc agctcgccac ccccgcgaaa gccgacgaca
      601 agccagcccc gccggcgact actgtggctg ttgccaccga catcggtgct tcggaagacg
      661 tcgcggccat ctcggatgcg gccgaggact tcgaagcggg caaccccgcg accgtggccg
      721 atcttgcgga tgcggcatca gcggctgatg aggttccgct caagaagacc aagggcggtt
      781 ttgaggtggc caatgacgac ggcgccgaga tcacggtcac gaaggatggt gctaccacct
      841 tgtcggcccc gggggttcct gagattggga tcagcgtcgc gggcgacgcc gacgctagca
      901 agctggtcga tggtgtgttg gttcagaccg aggtcgcgcc cagcactgac gtggttacgc
      961 gcgccaccga caacggtgtc cagttgatcg ccgtactggc ggacgcggat gcgcccgacg
     1021 agatcgagtt cccactagag ctgccgagag gaggcgaact agtcgaactg gccgacggcg
     1081 cccttgctgt gatggctccg gtgacccggg ttctggctga tccgggcgag tgggagcgag
     1141 tcaaccggga ggttgagtcg attctgggcg ccaactacga cgtgagcgtc gatccgactc
     1201 ctgcgcagga gaaagctctt gctagtattc cgccggtctc gacccacgaa gaggtcacgg
     1261 ttgagcaggt agcggtcgtt catgctccct gggctgttga cgcgaacggg cgagccgttg
     1321 acacgcacta tcgggtggac ggaaatactc tcatccaggt tgttgacgct ggcgacgatg
     1381 ctgcgttccc gatcaccctc gatcctggcg tggattggtg gaaggtcacc aagtgcgtcg
     1441 ctgcgatcgc gtgggcagtc ggctccacga tcttcgcggt cgccaagatc gccaagatcc
     1501 ggaagtacat cgaggcgctg ggtggcatca ctcagactgc aaaactggtc atgggagcga
     1561 ccaccaaagc cgagaaagcc atagccatcg gtaagggact gctgacgttc gctgctatcg
     1621 tcttcgggat tgatcagatc tacgagaatt gccgaccatg attccagtgc aagaactggt
     1681 gtgggtcgtt agcgcgttcg tgttcgcagg cggagtgatc tggggtgtct tcagaggccg
     1741 cgggcgtccg gacgtcgtcg attcggaccc ggccgacagg acggtgcacc gagatcaaga
     1801 gtacggttca gaccttgcat tcgtcggatt ctcgctggcc gcgttcgtgg ccgtgtacat
     1861 cgtccgcgca tggggcagtt gggactggtc agttccaatg attgctctcg taggcctagc
     1921 cggtgcccgg ctcgcggacg tactccgttc ggccaaagct gcccaatggg ttccatgggt
     1981 gttcgtcgcc gtcgccgcgg ggctgctcgc gatctagttg gtgcgccaag gaaccgcctc
     2041 ggcctgatca gtcgcgcatg ggtcccaccc gattaggtgg ggccctttgc gttgcccgga
     2101 caccaagcag acaaggcaag cgcctgcgtc ccaccgctca agcgatcact tcgcggcaag
     2161 cgagacaggg cggcaactgg atgaagaagt cctcatcatc ggctcacacc ggtctcaccc
     2221 gcgtccggtc aactagtgcc atcgacaaca cgaaccgtct gtccgaagcg gacggcaacc
     2281 aaaggagatg gacatgttcg caggacgtat tcgcaccagc ctcaccgctc tcgccgtgat
     2341 cggcgcaggc gccggactgg cactagtcgc cccgacggcc gccaccgcag ttccgctgcc
     2401 gatggccacc gccacctgca acgggctggc tgccacgatc gtcggcaccg aaggctcgga
     2461 cgacatcgag ggcaccgccg gacgggacgt gatcgtgacc ttcggcggca acgacgacat
     2521 cgacggcaac ggcggcaacg acgtgatctg cgctggcagt ggctacgacg aggtcgacgg
     2581 cggcgaaggc aacgactgga tctccggcgg ctcgggcggc gacacgctcg aaggcagctg
     2641 gggcgccgac aagatctacg gtaactccgg caacgaccac atcgagggcg agtcgggcaa
     2701 ggacaagctc tacggcggtt ccggccacga cctgctcgaa ggcggcagcg gggccgacaa
     2761 gctgagcggt ggctccggca aggacaccac ggtcgcgcgc tacgcgtccg atcgcaccga
     2821 ggaccgctgg gagaaccgct acggctacga cgactgacgg ccatcacgct ggctgagtcc
     2881 cacgctggct gagtcctaac gctggctgag cctgtcgaag cccccgacgc tcccgaggca
     2941 tcccgccccg ggagcgtttc ggccgtgctg gcgcgtaatg gaaccgtccc catttcgcgc
     3001 catcggttga caaggcaatt cgatctgtcg agcacctcgg tttggcggga aatggaaccg
     3061 tccccaatta ccagccaaga tcgtcctttc ggatgattcg ccgcgccgcg caggaggacg
     3121 ccgaccgccg atgccgttcg taggctgacg ggcatgatcg aaccgacctt gatggaagcg
     3181 ccgcccccgg gcatcgaggc gatggggctg gcgcgggcat tcggtgacgt ccgggccgtg
     3241 gacggcatca ccttcaccgc tcccgccggt gcggtgactg cgctgatcgg accgaacggc
     3301 tccggcaaga ccacgctgct gctcctgctc agcggcttgc tcaagccgga cgcgggcaag
     3361 gcgatcatcg ccggtcacga cgtcgagtcc gagaacctga gcgcgcgttc gcacgtcggc
     3421 tggatgccgg acgccttcgg cacctgggat gcgctcacct gcaccgagat cctcaccacc
     3481 ttcgcgcacg cctatggcat tccgcgtgcg ggcacgctcg ggttggtcga cgacctgctg
     3541 cgtcgagtgc acctgactga gttcgcgcac cggccggcgc gggtactcag ccgcggccag
     3601 aagcagcggc tcggcctggc cagggcgctg gtgcacgatc cgcaggtact gctgctggac
     3661 gaacccgcgt ccgggctcga cccccgctcc cggatcgaac tgcgggatct gctccgcgac
     3721 ttggcgcgtg agggcaagac cgtgctggtc tcctcgcacg tgctctccga gctcgaagag
     3781 ctctacgacc acgcggtctt cctctcccgc ggacggacgg tggacgcgtc cgcggccggc
     3841 gcgtccgggt ccgtccagcg gggctggcgg ctggacgcga tcgacccggt aggcctgcgc
     3901 acgttcctcg acgacgccgg catcccctgg acgggccagc ccaacggcga ggtcgtcgtg
     3961 caactctccg gctatgactc ggcccgtgag ctcgtccggg cagcggtagg cgcggacgtg
     4021 tcgctgcaca ccatctcgcc ggtctccgga cggctcgagg agacctacct ttcgctgaac
     4081 gaggagcggg tatgaacacc tggacgctga gcttcggtgg cctgcgcacg gtcatcgaac
     4141 tggaactcaa gcagcggatc cgctccaagc gctggatctg ggcgctggcc ggctggttcg
     4201 tgctgctcgg cgcggtgacc gggctgatca tctgggccgc gcacgagatc tactcctggg
     4261 acaacaacaa cgtccaggcc ggaccgatgg ccttcggggt gatcacgttc ttcgtcctgg
     4321 gcatgggact actgatcgcg ccgactttct cggcgatgtc gatcaacggc gatcgtgctg
     4381 gcggcacgct ggccaccctg caggccaccc ggctgagcgc gttcgagatc gcggccggga
     4441 agctgctggc tgcgtggctg accgcggcgg tcttcctggt ggtggcgctg ccgttcatcg
     4501 tctggtcgat ggtgttgggc aacatctcgg tctggcaggt gctggtggtc ttcggcgtgg
     4561 tgctggcaga ggtggccgtg gtctgtgcga tcgggctcgg ctggtccgcg gtgacctcgc
     4621 ggccggccgg gtctgcggtg atgacctatc tgagcatcgc gttcctgacc gtcatctcca
     4681 ccatggtgat gggcctgctg ataccgatgg tgaccgatga acagcccgtc cgggtctggg
     4741 gactgaccgg tcaggtgcag gacgactaca accgcgcgct cgacaagtac tgggcagcca
     4801 acccggacgg tgacggcacc ggcatgcccg cggcacccat cggtaagtgc acctggttcg
     4861 tggagaacca cacgatcacc cacgccgatc gggtgtggtg gatcactgtg gtgaacccgt
     4921 tcgtgatcgt ggcggacgcg gccccgctgc cgccgggtgc ggcgaagaac ctgagccagt
     4981 accagtcgct gggttcggac ccgttggcca cgatccgctg gggggtgcgg cagttgagca
     5041 tgccgccgca gctcgagttc gaccagtgct cgcagtacta cgacgggatg cccggctaca
     5101 cggtcgtcta cgacgaccag ggcaacgcca tcgtcaccac gaccaagggt ggtgcgacgg
     5161 tgaacgtgga gagcccggtc aagcgccgag tggtcaacgt cgagaccccg atctggcctt
     5221 ggggacttgc tgcgaacatc ctgatcggcg gcgttttctt ctggatcgcc gtacagcgcc
     5281 tgcggattcc ctacgccatg cttcccaagg gcacccgcgt ggcctgagct gtcgacactc
     5341 agcgggagcc ggccagaccg gactcgtagg cgaccaccac ggcctggacg cgatcgcgga
     5401 cctccagctt ggtgaggatg tgccgcacat gggtctttac ggtcgtctcg gagaggaaca
     5461 gttcagcgga gatctcggcg ttcgacaggc cgcgcgcgat cagctccagc acttcgcgtt
     5521 cacggtcggt gagacactcc acccggtggt cgggctcaag ggtcggcgcg ccgacaccgg
     5581 tagccacatg agccagcagc cggcgggtgg cggaaggggc caccaccgcg tccccgtccc
     5641 gaaccgtacg aatggcggcc agcaggtcgt ccggcggcgc gttcttcagc aggaaaccgg
     5701 acgcgccggc cttgatcgca gccaacaggt attcgtccaa gtcataggtg gtgagcatca
     5761 cgaccctggg cggctcgtga ccggccagat cggccgcatc gatcagccgc cgggtggcct
     5821 ccaacccgtc caggtggggc atccgcacgt ccatcagcac cacgtccgca gcacaatcgg
     5881 cgagcaggtc cagcgcctcc agcccatcgg atgcctgccc ggcgacggtc atgtcggtct
     5941 gggcgttgat caccatcgcc agcccggcgc gcaccagatg ctggtcgtcg accagcacca
     6001 cgcggatcgg ttcggtggca ggctcaggca tgcgtctcct ccaatgaagc ggcgagcgga
     6061 atgatcgtgc gcacccggaa cccgtcctcg gtctcgcccg catcgaaggt cgcgccgatc
     6121 agctcggccc gctcccgcat gccgatcagg ccaaggccca ggcctgataa cggctcttcc
     6181 gcgtcgggat caagggtccg agtgccctca ccgtcccggt cggtgacggt cagcgtgacc
     6241 tcgtcgtcca cccagctcac cgcgacgatc accttggcgc cttcgccggc gtgcttgatc
     6301 acgttggtca gcgcctcctg gcagatgcgg tacagcgcca gccgcgaccc cgaaggcatg
     6361 ccacgcggct ggccgacccg gatcagcgac acctgcagcc cggcctggcg agcctgctcg
     6421 atcaattcgt ccagcgaagc ctgcgtcggc gtcggcgtca gttcggcctc ctcgccgtgg
     6481 cgcagcatgc ccaggatgcg gcgggtgtcg gcgagcgcca cccgtccggt ctcggcgatt
     6541 gtgacgagcg cgcgtttggc ggcggcgctg tcggccacca cgtaactgcc gccgtccgcc
     6601 tgcgcaatca tgatcgccag cgagtgcgcg atcacgtcgt gcatctccct caggatgcgc
     6661 atccgctcgg ctgcggtcgc cagttcggtg gcctgcttct gcgcgttcac tgcccggtgg
     6721 cgggccgcga gggcttcgcg cagctccgtc cgggtgcgtt ggtggacgac gaacagcgcc
     6781 gccagaccta cggccagcgc cacggctgcg accagcagca ccacctcgag cggcgacatg
     6841 ggcttaggag tgaacgacca cttccacccg ctggaactcc ttgacgtcgg agtagccggt
     6901 ggtggccagc gcccggcgca gcgcgccgac caggttcatc gtgccgtcgg cgacgtggga
     6961 cggtccgagc aggatctcct cgagcgtgcc gaccgcgccg aacttgaccc gctcaccgcg
     7021 cggcagcgtg gcgtgccagg cctccggtcc ccagtggtag cccttgcctg gcgcctcggt
     7081 ggcgcgggcc agcggggagc cgaccatgac cgcgtccgcg ccgcaggcga tcgccttggc
     7141 gatgtcgccg gacttgccaa gggcaccgtc cgcgatcaca tggacgtagc ggccgccgga
     7201 ctcgtccagg tagtcgcgcc gggcctcggc cacgtcggcc agcgcggacg ccatcggcac
     7261 ctcgacgccc agcacgttgc gcgtgcgctt ggtcgcgcca ccgccgaagc cgaccaggac
     7321 gccggccgca ccggtgcgca tcaggtgcag tgccgactgg taggtggcgc agccgccgac
     7381 gatcaccggc acgtcgagtt cgtagatgaa cttcttcagg ttcagcggct cgcaaaccgt
     7441 ggagacgtgc tcggccgaga ccgtggtgcc gcggatcacg aagaagtcca cgccggcctt
     7501 ctccacgacg ctggcgaact cggcgcaggt ctgcggggac agcgaaccgg ccaccggcac
     7561 cccggcctcg cggatctcgg ccatccggcc ggtgatcagc tcagccttga tcggctcggc
     7621 gtagagctcc tgcatccgcc gggtggcagc gagctggtcg tcgatgtcgg ccagttcggt
     7681 gagcagcggt tccgggtcgg cgtagcgcgt ccacaggcct tccagattga gcacgcccag
     7741 gccaccgagc cggcccatcg cgatggctgt ggccggcgac atcaccgaat ccatcggcgc
     7801 ggccaccatc gggaagtcga agttgagcgc gtcgatcttc cagctgaggt tcacctcttc
     7861 gatgccgcgg gtgcgccggg tgggcacgat ggcgatgtcg tccaacccga agacgggcag
     7921 ggcacgcttg gaacggccga tctcgatcat gatgagctcc cttgctcagg actgggacgt
     7981 gtagttgggt gcttcgatgg tcatctggat gtcgtgtgga tgcgactcgc gcaggccggc
     8041 ggcggtgatc cggacgaagc gtcccttggt gtgcagttcg tcgatggtgc tggcgccggt
     8101 gtagaacatc gactgccgca gcccgccgac gagctggtag gcgaccgcgg acagcggccc
     8161 gcgataggcg acctggcctt cgatgccctc ggggatcagc ttgtcgtcgc tggcgacgtc
     8221 gccctggaag tagcggtcct tcgaatacga catcttgcgt ccgcgggcag ccatggcgcc
     8281 cagcgagccc atgccgcggt acgacttgta ctgcttgccg ttgatgaaga ccagctcgcc
     8341 ggggctctcc tcgcaaccgg ccagcagcga gccgagcatc accgtgttcg cgccggcgac
     8401 cagggctttc gcgatgtcgc cggagtactg caggccaccg tcgccgatta ccgggacgcc
     8461 ggccggacgg cacgccgccg cggcctcgtg aatggcggtc acctgtggga cgccgacccc
     8521 ggcgaccacc cgggtggtgc agatcgagcc cggtccgacg ccgaccttca ctccgtccgc
     8581 gccggcttcg accagggcca gggcgccggc gtaggtcgcg atgttgccgc cgatgatgtc
     8641 cacctcggcc gcggccggct cggccttcag ccggcggatc atctccagca cgccctgcga
     8701 atgcccgtgc gcggtgtcga cgacgatcgc gtccacgccg gcgtcgacca gcatcatggc
     8761 gcgctcccag gcgtccccga agaagccgat cgcggcgccc acgcggagcc gaccctgcgg
     8821 atccttgcta gccatcgggt acttgtcgga cttcacgtag tccttgagcg tgatcaggcc
     8881 gcgcaagcgt ccgtcggcgt cgaccagggg cagcttctcg atcttgtgct tggccagcag
     8941 ttgcagggct tcctcgctgc tgatgccggg ccagccggtg accagcggca tcggggtcat
     9001 cacttcgccg accggacggt tcgggtcgtc ctcgaagcgc atgtcgcggt tggtgacgat
     9061 gccgagcagc ttcaggtcgg aatcgacgac gggcaccccg gagatccgga agtgcccgca
     9121 catctcgtcc acctcgccga tggtggcgtc cagggtggtg gtgatcggct ggtcgatcat
     9181 gcccgcctcg gaacgcttca cccggtcgac ctggccggcc tggtcttcgg cggacatgtt
     9241 gcgatgcagg atgcccatgc cgccctcgcg cgccatcgcg atcgccagcc gcgactcggt
     9301 gacggtgtcc atcgccgagc tgagcagcgg tacccgaagc tggatgcgct tggtcaccca
     9361 cgtggtggtg tcgaccgcgg acgggatcac gtccgactcg ttgggctgca gcagcacgtc
     9421 atcgaaagtc aggccaaggg cagcgaacgg cccaggcact ccggacgggg tgagctcaac
     9481 gggactcgac acatcacacc aatcagtcgc tcaacgtgga agtagggagt ctaccgccga
     9541 ggcggttggt gtccccggtc agctcagggc ggcgaacgcc tggcggacgg tcttgtgctc
     9601 gaactcgaag ccggcgtcca gcaaatgggc cggacggacg cgctggccgg gcagcaacag
     9661 ttccttcacg aactcgcggc cgaagacgag gccgaccgca cccagcggca gcggcagcgg
     9721 cgtgggtcga tgcacgtgcg aaccgaagac gcgggtgaac tcggcgttgc tgaccggctc
     9781 gggagcgacc aggttcacgg ggccggtgag ctcggaatcc agcagaaaag cggtcgcgcg
     9841 gacgtgatcg gcgaggctga tccacgacac gaactggcgg ccgctgccca cgcgtccccc
     9901 cagtcccagc gcgaacgccg gccattgcgc ggcgaggtag ccgccgtggc gggccagcac
     9961 gtttccggtg cgcagcagcg cggtagatac cggggagtgg gcggccgcgg cttcccagtc
    10021 ggccaccacg ccggccagga aaccgcgtcc gacgggcatg gtttcgtcca cgatctcggt
    10081 gccggtgtcg ccgtaatacc cgatggccga gccgttcagc aggcgctggc agcgtccgcc
    10141 tggcgacagc gaggtgacga tggtgagggt cgatgtgatc cgtgagcgtc ggatctcggt
    10201 cttgcgatcc ttggtccagc ggccgccggc gatcgacgcg ccggcgaggt taaccaccgc
    10261 atccacgtcg tcgaggccgg gggagtcgat gcgtcccgcg tccggatccc agcgccgctg
    10321 gtcggacgcg ctgatctcgc cccgcaccag ccggaccacc tcgtgcccgg cgccgaccaa
    10381 gtgccggctg agtgcggaac cgatcagtcc agtgctgccg ccgatagcca ctcgcatacg
    10441 gtgatcctgc cacccagggt caagtggacg tgtgcctggc caaggcgatc tcagacctgg
    10501 ccgtgccaaa tcacggtctg cagcgcgttc cagatgcaga acgcgatcgc gaagccgaac
    10561 acccaatagc gttggcgccg gttcagcgga atcaaaccca gcagcgcgag cgaccacggc
    10621 aggtaccacg ggtgcagcgc ctggccgagc actgcgatca ccagcgatgc ccaggccacc
    10681 agcgcgagcg gacggttggc gaagcggaca ccgagccaga ccagcaccgc gagcaggatg
    10741 gcggtgccca cccgcccggc cacgatcagg aaacccgtgt acgagccgcc ggcggcctgc
    10801 cagatcagcg cgccgccctt gctcagtagc gccagcggtg ccggggtgcc ggccaggccc
    10861 atcaggtcga gccactcgac ccagccgaag cccagcccgc tcgcgaggct gacacccgcg
    10921 aagaccacga gcgtcactgc cgtggcgagg gcggttcgcc agccgagcag ccacagccga
    10981 cgcgccatcg gggcggcaac caaagcagcc gcgaccggca gcccggcaac cgcgatcacg
    11041 gtgagtccac cctgctgctt gaacgccatc gcgagtgcca cgaccgctgg gccgaccagg
    11101 aatgatgccc accacttcgg ccagcggcag accacccaga cggcggccag cgaaagggcg
    11161 accatcggtg cgtcgttgtg ggcggcgccg atgaagtgca gcagcagcag cgggttgagg
    11221 acgcccaacc agaccgcgcc gcgcgccggg cgtacgagca gcttggcgat gcgtggcacc
    11281 aggacaacca tcgccaccac cgcgaccacc gccgggattc gcatggcgat caccgaccag
    11341 tacggatgca gcccggtcgc ggtgacgatc agctggttca ggtgcaatga caagggggga
    11401 tacgccacac cgctgcccgc ccagagtggg tcgacccagg ccgcgaacgg tccaccagag
    11461 gtcgccaggc cgacttcgta ggggttgtgg ccggcgtgca gcgtccaacc caggtcggcg
    11521 tacagcaccg cgtccgggct gagcaccggg ggtactagca gcaacgggag cgaccagagc
    11581 agcatcgccg taccggcatg gacgggcgcg cggtcgcgcg tcggccggat gtgccaccag
    11641 gcccaggtaa gggcgatcac cccgagcacg atcaggatgc ggttgaccgg ctgcggagcc
    11701 gactctccga tcgcgttgat cagctcggac ggccagccgt atgctgacgg ggagaattcg
    11761 gggtggcagc tgccccaggc gacagcgaac gagccgagcg tgcctaccca cactccctgc
    11821 gaccgcagtg cggtggcgat cgggatgggc atttcgctcc aggactcggt ttgaggttgc
    11881 ccattgagct taccgacccc ggtaggttgg cttcatcgaa gggggaaaag gtggagttct
    11941 cggggatggg cgcggtggtt acgcccgagg gagtcggctt ccgggtctgg gcgccgcacg
    12001 cctctgcggt cgcggtggtc ggtgagttca acgactggac gtccgatgcc aatcagttag
    12061 cgggcgaagg caacggcaac tgggctggtt ttgtgccgga cgcgaaggcc ggccaggagt
    12121 acaagttcca gatcaccaat ggcgagcagg tgctcaccaa ggtcgacccg tacgcggcgc
    12181 acgtcaccaa ctcggtgggc aacggggtga tctacgacca cgatgcgttc gactgggccg
    12241 gggatgagtt cacgccgccg acgttcaacg aactcgtcat ctacgagttg cacgccggga
    12301 cgttcgccag ccaggcggac ggtgaggtca gcgatctcga cgaaatggcc gagaagctcg
    12361 gccacatcgt caagctcggc gccaacgcga tccagctgat gccggtggca gagttcgccg
    12421 gcgactattc ctggggctac aacccggcgc acatcttcgc ggtggagagc gcctacggcg
    12481 gtccggacgc gttgaagcgg ttggtcaagc aggcgcacgc gctcggcctg gcggtgattc
    12541 aggacgtggt ctacaaccac ttcggtccca gcgacctgaa cttgtggcag ttcgacggct
    12601 ggtcggaaga cgataagggc ggcatctact tcttcaacga ttggcgctcg acgacgccgt
    12661 ggggcgacac gcgtccggat tatgggcgcg gcgaggtgcg ccagttcatc cacgacaacg
    12721 cgttcgcctg gctggagcgg ttccatatgg acgggctgcg atacgacatg acgccctaca
    12781 tgcgcgatgt ggacggctcc ggtttcgaca ttccggaagg ctgggatctc tgtcgctgga
    12841 tcaacaccga cattcgctcg aagtacccgg agaagatcct gatcgccgag gacctgcatt
    12901 cgaacccggc agtgagcagc acggggcccg agggggcggc cttccacgcg cagtgggacg
    12961 cgcagttcgt ccacccggtt cgggaggcga taaccgtggc cgaggatcag taccgctcca
    13021 ccgacgaggt gctgggcgcg gtcggctact cctacgggga cgcgttcgcg cgggtgatct
    13081 acaccgaaag tcacgacgag gtggcgaacg gaaaagcgcg ggtgccggag gagatcgacg
    13141 cggacgatcc gaccggctat ttcgcgaaga agcggtcgac gctgggcgcc gggctggtgc
    13201 tcacctcacc ggggatcccg atgctgttcc agggccagga gttcctggaa ggcggctggt
    13261 tccgcgacaa cgtggccctc gattgggatc gcagcgacga cttccacggc atcgtgaagc
    13321 tgtatcggga cctgattcgg ctgcggctca accgggactc gttgagcgcg ggactgaccg
    13381 gttctggcct gcgggtgatc cacaacaatg acgccgagaa cgtgatcgcg ttccagcgct
    13441 ggcgtgagca cggcgtgggt gacgacgtgg tggtgatcgc gaatctgtcc aaccagaccc
    13501 ggacggacta ccgcatcggc ctgcccgctg ccgggaagtg gcagttgctg ttcgacgggg
    13561 attccgcgat gtacagcgaa gacttcggcg aacacgcggc ccgtgacctg gtcgcgtccg
    13621 cggaggccta cgacggtctg gacgcgagcg ccgagatcag catcgccccc tacaccttgc
    13681 tgatctacgg ctggcgcggc tgagttcagt cgccccggac gtccactcgg ctggtcgagc
    13741 ctgtcgagac cgggttcaca ctccgccaac gagcagatgc tcgtagtgcc ggaaccgccg
    13801 cggcgcaccc ttcagctccc actcgctgaa ctcgtccaga gggcggtaac cggcggcctc
    13861 ggcaaccacc accgaagcgc ggttcgcgtc ctcgatctgc aggtgaagcc ggtcgaggcc
    13921 gagctcgcga tgcgcccacg cggtgatcaa ccccaacgcg gttcgggccg cgccgcgtcc
    13981 gcgcgctgcc ggtgccaccc agtagccgat ctcagcggcg gacccacttt ggttcacgtc
    14041 catcaccgag caggcgccga tcaattcatc agcggcgtcg gcaatggcga accagctgcc
    14101 ctcgcgactc tgccaggcgt gcccggcgag gctgacgaac gacctcgcgt cggcttcggt
    14161 gtacggcact ggaacccggg tgaagcgggc gatctccgga tcccggcacg ccgcgaccaa
    14221 attcgacacg tcccgatccg tcagcggacg cagcgcgcac gcggttccca caagcctcgg
    14281 ctgcgatgct ggccacatgc ccgcatccta cggatgccgc aggcggccac ggttgtcgag
    14341 tcgggcgaaa cccaaccacc ttcggctggt cgagcttttc gagaccggag tgggaacggt
    14401 ggctgagctt gtcgagacca ccccttcgtc aagctcaggg atcgtttggc aggtgtcagg
    14461 gatcgtttgg ctgacgtcgg ggatcgtcgg ctcggctact ccggtccgct agcttggctt
    14521 ggtgcgcgcg cccaaccagc agaaccggga gatcctcgcc ctggcgatcc ccgcgttcct
    14581 cgcgctggtg gctgaaccgc tgttcctgct agccgacacc gcgatcgtcg ggcacctggg
    14641 cacggcgcag ctggccgggc tcggcgtggc cagcgcgatc ctgctgaccg cagccaacct
    14701 cttcgtcttc ctggcgtacg gcaccacgtc cgtcgtggct cggcaggtcg gtggcggcgc
    14761 tcgcggcgct gccctcagct cgggtgtgga cggggtctgg ttggccatcg tgctgggact
    14821 ggtggtggcg gtcgtcgtcg gcggtctggc tgagccattg tgcgcggctt tcggcgccac
    14881 gccagcggtg ctcgccgaag cgacgatcta tctgcggatc tcggcgatcg gattgccggc
    14941 gatgctggtt gcgctcgccg ccaccggcgc gttgcgcggc cttcaggaca cccggacgcc
    15001 gctgatcgcg gcggtggccg gtttcggggc aaacctggtg ctcaacctgt ggttcgtgct
    15061 gggcctgggc tggggcatcg ctggttccgc ctggggcacg gtgatcgccc agaccgcgat
    15121 ggccgtcgcg ctggtcgcgg tgctgattcg cttggccgga cgcgaacacg cgacgttgcg
    15181 tccgcatcct caccgggtgc tggcggctgc gcggaccggc gtcccgctgt tgatccggac
    15241 gctggcgctg cgcgcggtca tcctggtcac cacctgggtg gcggccggtc tcggcgacgt
    15301 cgccctggct gcccatcagg tggcagccac gatctggact ttccaggcgt tcgccctgga
    15361 cgccctggcg atcgccgccc aggcgctgac cggcaagggc ctcggggctg gtgatcgggc
    15421 cgcggtgcgt gccgcgacca ccaccatgac ccgctggggg ctttggggcg gggtggtgct
    15481 cggcgggctg ctggtactgg ccagcggggt gctgccgccc ctgttcaccg ccgatgcagc
    15541 ggtgcaagcg gccttgtcct gggcgctgat cgtggtcggt gtgggtggac cgctctccgg
    15601 actggtgttc gtggtcgatg gcgtcctgat cggggccggc gatggccgct ggctcgcgtc
    15661 cgcgatgctg gggacgctcg cgctctacct gccgctggcg ctgctggttc gctggcaggc
    15721 gccggcgctg atcggtctgg gtgcgccggt cggcgtggcc gtgctctggg tcgcgttcct
    15781 cgcgttcatg gcgatccgct ggggcttcct gcgccaccgc gtccgctcgg acgtctggct
    15841 ggttaccgcc tcctgactcg gtcgctgtct ctctgcgttc tgttccggac agagtggacg
    15901 gttcctcggt gggacgccgc gaacccgtcc acctttatgg atccacgcgc gccggcagcc
    15961 gcggacgctt actccgcggc gatggcctcg tggtgccgca cgacttcagc aacgacgaac
    16021 tccagccact tctccgcgaa ttcgggatcg aggttcgcct gctcggctag cgcccgcagt
    16081 cgcgcgatct gctgcacttc gcgctcggga tccgccggag ccagcccgcg ctccttcttc
    16141 aggaccccca cgcgctgggt gatcttgaag cgctcggcga gcatgcagat gatcgcggaa
    16201 tcgaggttgt cgatcgagcc gcgcaatcgt tccagttccg ggtcgctctg cacgcccctc
    16261 agcctagagg acccgcgctg ccagcgtccg cgccgacggc cgtcccagtt cgtccgacgc
    16321 attgacgcca gcgaaacgga cgtttgccgg ggaacattcc ccggcaaacg tccgtttcgc
    16381 tggcgggaac gcctggacgg gccgcgagtc cggacggccc ggacggctca gtcgagcagg
    16441 tcggcgtaca ggatcgtcga gaggtagcgc tcgccgaagg acgggatgat caccacgatc
    16501 gtcttgccgg cgttctccgg acgggcggcg agttcgcccg cggcggccag ggcggcgccg
    16561 gacgagatgc ccaccagcag gccctcctcc ttggcggcgc gacgagccca ctccacggcg
    16621 gtctcggcgt tctgcggcag cacctcgtcg atcaccgagc gatccaggat ctccgggatg
    16681 aagttcgcgc cgatgccctg gatcttgtgc ggcccgggct gcccacccga cagcagcggc
    16741 gactcggccg gctcgaccgc gacgatcttc acctcgggct tgcgctcctt cagcacctgg
    16801 ccgacgccgc tgatcgtgcc gccggtgccg acaccggcca cgatgtagtc gacggcacct
    16861 tcggtgtcgg tccagatttc ctccgcggtg gtgcgacggt ggatgtcgac gttggccggg
    16921 ttggcaaact gcttggccag gatggcgccg ggggtctccg ctgcgatctg ctcagcccgg
    16981 ttcaccgcgc ctcgcatgcc ctcggaaccg ggggtcagca ccagctcggc gccgaaagcg
    17041 cgcagcagcg cccggcgctc cttgctcatg gtctcgggca tggtgaggat caccttgtag
    17101 ccgcgggcgg cgcccacgaa cgcgagcgcg atcccggtgt tgccggaggt gccctcgacg
    17161 atcgtgccgc ccggcttgag ctcaccggac gcctccgcgg cgtcgatgat cgccacgccg
    17221 atgcggtcct ttaccgagtt ggccgggttg tagaactcca gcttggccag cacgatggcc
    17281 tgggagtcgc cgtagaggcg gttgatcttc actagcgggg tgcggccgat gagcttggtg
    17341 gcgtcttcga agtaagccat ttcgtttcct tgtctgtttt gtcttgcgag tcgcggtagg
    17401 tcgagggtct ggtcagagct gggcgagggc ctggtcgagg tcggcgatca ggtcgtcgat
    17461 gtgttcgata cccactgaga gccgcaccat ctccggagcc actccggccg cgaccaattc
    17521 gtcctcggtc agctgagagt gtgtcgtggt ggcgttgtga atggccagtg acttggcgtc
    17581 cccgatgttc gccaggtggg agaacaattg cagagcctcg atgaacttgg cgccggcttc
    17641 gcgtccggcc ttgagcccga aggcgaggat gccgccgtag ccgcgtccgg tgaagacccg
    17701 gtcggcgatc gccttggacg gcgaggattc caggcccggg tagttcaccc aggcaacctg
    17761 gtcgtggttc tccaggtatt gcgccacggc cagtgcgttg gtcgagtgcc gctcgatccg
    17821 caggtgcagc gtctccaggc cctgcaagaa gagccacgag ttgaacggcg tggtggccgc
    17881 gccggtgttg cgcagcagca ccgtccgggc ccggatgatg taggcggccg ggccggcggc
    17941 gtcggtccag accacaccgt ggtaggccgg gtcggccgca gtcaggccgg ggaagcggtc
    18001 ggcgtgcgcg gcccagtcga acttgccgga gtcgacgatc acaccgccga tggacgtgcc
    18061 gtgcccaccg atgtacttgg tggcggcgtg cacggcgatg tccgcaccgt gatcgaagac
    18121 ccgggccagc aggggagtgg gcacggtgtt gtccacgatg aacggcaggc cgagggcgtg
    18181 cgcggcctcc gaccaggcgt ccaggtcgat cacgttgatc gccgggttgc cgatggtctc
    18241 accgaagacc agcttggtct tggcgtccac gtgctgggcg aggtcctcag gcttgcctgg
    18301 atcgacgaac cgcacctcga tgccgaactg cggcagggtg tgggcgaaca gggcgtaggt
    18361 gccgccgtac agcgtggaca gcgccacgat gttgtccccg gcgtaggtca ggttgagcac
    18421 cgcgtaggtc accgcagccg aaccggacgc ggtcgcgagc gccccgacac caccctccag
    18481 ggcgttcacc cgggtctcga acaccgactg ggtcgggttc atgatgcggg tgtagatgtt
    18541 tcccggcacc ttcagagcga agagattcgc ggcgtgctcg gtgtcgtcga agacgtagct
    18601 ggtggtctgg tagatcggta ccgcgcggga gttggtggcc ggatcggcct cctcctgtcc
    18661 ggcgtgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncggg aggcgcaggt
    18781 ctactccgag atcgtcccgc acacgatgcc ggtcgccgag ttgctggcca ggcgtccggc
    18841 cgcggtggtg ctgtccggtg gcccgagttc ggtctacgag gccggtgcgc cccagttgga
    18901 cgcggccctg ctggccgccg gcgtcccggt cttcggcatc tgctacggct tcatggcgat
    18961 ggcccaggcg ctcggcggga cggtggcccg caccggtcag cgcgagtacg gccggacggc
    19021 ggtcagcgtc ctcgagcccg gacgcctgct cgccgggctg cccgaaacac tcacgtcctg
    19081 gatgtcacac ggcgacgagg tgaccgcggc cccgtccggt ttcatcgtga gcgcgcgctc
    19141 cccgcgggcc acggtggcgg ctttcgagga tgccgagcgc aggctggccg gcgtccagtg
    19201 gcaccccgag gtgctgcatt ccgagcacgg ccagaaggtg ctggagcact tcctggtcga
    19261 catcgccggc tgccgccaga cctggaccag cgccaacatc gctgaggaac aggttgagcg
    19321 cgtccgcgcc gaggtgggcg acgcccgcgt gctctgcgca ctgtccggcg gcgtcgattc
    19381 ggcagtggcc gctgccatcg tccagcaggc catcggtgac cagctgactt gtgtcttcgt
    19441 cgaccacggc ctgctgcgca ctggcgaggc cgagcgggtg gagcgcgact tcgtggcgtc
    19501 caccggtgtg cacctggtgg tggccaatga gcaggagcgc ttcctctcgg cgctggccgg
    19561 ggtcagcgat ccggagacca aacggaagat catcggccgc gagttcatcc gcaccttcga
    19621 ggacgaggcc cgcaagctga acgccgaggg cccgatcgac tacctggtac agggcacgct
    19681 ctatccggac gtggtcgaat ccggcggcgg cgagggtgcg gccaacatca agagccatca
    19741 caacgtgggc gggctgcccg atgacctgca gttcaagctg atcgagccgc tgcgcaccct
    19801 gttcaaggac gaggtgcgcc aggtaggtcg ccagctcggg ttgcccgagc acatcgtcga
    19861 ccggcacccg ttcccgggcc ctggcttggg gattcgcatt gtcggtgcgg tggacgccga
    19921 acggctggcg atcctgcggg cagcggacgc gatcgtccgg gaggagacca cagcagccgg
    19981 attggacggc gagatctggc agttcccggt ggtgttgctg gccgacgtcc gctccgtcgg
    20041 cgtgcagggg gacggacgca cctacgggca cccgatcgtg ctgcgtccgg tctcgagtga
    20101 ggacgcgatg accgccgact ggtcgcggct gccctacgaa ctactggaga agatctccac
    20161 ccggatcacc aacgaggtgc ccgaggtgaa ccgggtggtg ctggacgtca ccagcaagcc
    20221 acccggcacc atcgagtggg agtagggccg accggccctt gggtcagcgg tagtcggtgg
    20281 gcgggttgtt gctgttgctg gtgaaggtgc ccttcaggtc gtcgagaccg gtccggcccc
    20341 cgtcctccag cggcaggacg agccaggcca cgccgtaggc gatcggcacg ccccagggga
    20401 agatcagggc agcgaccacg gcgaggatgc ggacgatgct gccttcccag ccgaaggagc
    20461 gggccaggcc gccgcagacg ccggcgatga acttgtcgga actcgaacgg gtgagtttct
    20521 gcttcatgat gctttccttt cgtgaaactc agttggactt gcgcgagagc aggagcatcc
    20581 ccgcgccgat gatgaccagg accaccgggg cgatcacccc gacgagtcgc cagtcgacga
    20641 acccgtaatg cagccagagc gccagtccgg cgatggccat ggcgatgacg ccgaatgcga
    20701 aggcggaacg atcggtcatg tccgcaccgt cacttcgccc atacccacgt caatgttcaa
    20761 ctggagcacc ggggtgccgg ttcccgagcc gaaggtctga cttcccttca cgtccactcc
    20821 gtcgttggag tgcaccgagt tgtcgaaggt gccggcgccg atgtgatagc tcacgagggt
    20881 gttcccgtcc gccggcagga tgatgtcgac ctcgcccgca ccgagtttga tgtccattgc
    20941 acgtccggaa tcgaggcgga tcgcgctcag atcgagggcg atctcgccgg cacccaggtc
    21001 gatctgccga ggcagctcgt cgacgcttcg gtacacctga gcggtctcgc cgacgctggc
    21061 cggcaccggc gacatcaacg cagcggtgag cagagcggtc acgatcgtca cgaacagcat
    21121 cccgcgcggg cgtccgacct tgctggacgc gaccagcgtc atcccgagcg cggccaggat
    21181 cgccgcaaag tagggcagcg gccctagcgg caacccgaaa accacgccaa gcacggtcac
    21241 cagggcgacg ccaacaccgc tcaatagcgc ggccagcaac cagaatcggc gacgccgctg
    21301 ccgggccgcc accatcgccg gcggcaggtt gtcgcggacg gccagatcgg cggggttggt
    21361 gtagggctgc tgccattgcg gagcctgctg ggacgtgggc aacgtcggcg cgggcaggct
    21421 ggccgcggac agcaggccgc tggcgggtgc atagcccggg gtctgctgct cggcgagtcg
    21481 atcgcgccag gcctcggcct gtcgttcgaa cggggtgggc tcgggggtcg gcgcgttcgt
    21541 ccgggtgccg tggttgcgga acccgaagta ccagaccgcg aagatcaccg ccaccgggcc
    21601 gaacccgatg ccgttggtcg gaatgccggt gagcaggacc agagcaccga tggttgcggc
    21661 cacgacgccg gcggtcggcc aatcgcgggt gaacgggagc catcggcgga ccggcatttc
    21721 ggtctgtccg tcgcgcggca tcaacagcac gccggcgccg taggccgcca ggccgagccc
    21781 accaaaaaga accagcactg caaccgcgat ccgaagcagg tttgggtcga catgccaacg
    21841 ccgcgcgagt ccagcgcaga cgccaccgat catggcgccg ttccgcggac ggctcagtac
    21901 gggcaggttg tcgggcacgg gtctattcaa cgccgctacc gcggccggca acatgggggt
    21961 ctaccctgag cggcaccgca ttttgcggcc ttgggatgcg acgcagattg aaccatcccg
    22021 ggctgcattc ggcctagtgg ctggcgggtc gagatgcgga atggaaccgt cccgggtcta
    22081 gaacgcctgg cacctccccg gggggtccgg aaagcttcca ctgggaacgt tttcatccta
    22141 gaagggcggc agttgcgagt cgccgcggta ccgcgtccga cttcggtatt gttcggtggt
    22201 caggcagtgc cggcgtccgg gcatccggcc cccgggtgtg tcgactgtcg aaggaggagc
    22261 ccgggtggtc aagacgctca acgctgatcg ttctgccccg acactggaag atgtagcccg
    22321 cgtggccggc gtgtcccgag ccacggtttc gcgagtggtc aacggcggaa acctggtcgc
    22381 caaggacacc accgaagccg tccgcagggc catcgccgag ttggcctacc agccgaatcg
    22441 cgcggcacgt tcgctggtga cccgacgcac cggggtgatc gctgtggtcg tgccggagac
    22501 cgacgaccgg gtgttcaccg atcccttctt cccgcaggcc tatcacggtg cgctgcaggc
    22561 attccagggc tcggacgtgc gggtggtgct ggccatggcc caaccgggcg acgggcccgc
    22621 gggcatggtg cgctacctcg aatccggcca tgtggacggt gcgatcatcg tctcgcacca
    22681 cggccccgaa ctcggccgcg ccctggcgag ctcctggcag ccggtggtct ttgtcggcga
    22741 tcccgaggcg ccaggcgtcc actacctgga tctcgacaac atgggtgcag cggcgaccgc
    22801 gacccagcac ctgatcgacc gcggttcgaa gcggatcgga gccatcaccg gaccgctcga
    22861 catgaactcg ggccggcgcc ggttgaaggg gttccagcaa gccatgaagg cggccgggcg
    22921 cgacgacacg ctctgggtgg agagcgacta cacccgaaac ggcggacgcg cggtgcttga
    22981 gttgctggaa cgggcccccg acctggacgg cgtgttcgcg gcgaacgacc tgatggcggc
    23041 gacggcgctg aacgtgctgc tggagaccgg acgccgggtg cctgaggacg tccgggtggt
    23101 cggattcgat gattccgagg ccgccctgca gaccaacccg cagctcacca cgatgaccaa
    23161 cccggcagcc gagcacaccc ggatggctgc cgagatgctg atctcgatcc tcgccggcga
    23221 gaatccgcag ccgatgacga agctgccgag caccctggtg gtccgcgggt cctcatgagc
    23281 gaagaggtcg tccacccgcg ctccacgcgt ccgctggctg aagcctgggt gggcggagtc
    23341 tgcgccggtc tggccgatca cctgggctgg tcggttctgg tgctacgggt gctgttcgtg
    23401 gtgaccgcag cattcgggct ggtcggggtg gccctgtacc tggtgctgtg gctggtgatg
    23461 ccgcaggagg tcgagcagcg ggacgcaccc gggcttgagg ccggcgcacg gaaggggctg
    23521 cggccgcgca caacgaagat gctgaccgcg gtcgacctgg gcgtggccag tgcccttgcg
    23581 ctgctcggcg tcggactggc ctggctggcg caggtctccg ggtacggacc gcccaactgg
    23641 ctgctcactg cgggtgcgct gggtgcgctc gggctcggga tcatctggtg gcaggccgat
    23701 cacgtctcca cccgcgaact gcgcaccggg accggctggc agcggctggt cgcgccgttg
    23761 gttgcgcact ggacgtcgat cgtgggcatc gtcgccgggc tggtcgcgct tggcgggtcg
    23821 atctgggtgg tgctgctggc ccaggcccga ctgagtgagg ctgcgcggac gatcctgctg
    23881 ctcggcctgg cggtgaccgc ggtggtgctg gctgcgctgc cgtggatcct gcacgtgcgc
    23941 cgggcgctcg cccaggctcg cgacgacgcg ctggtggcgg acgcgcgggc cgacatggct
    24001 gcgcacctgc acgactcggt gctgcagacg ctggcgttga tccagcggaa ggcgtccgac
    24061 ccgaaggcgg tgaccgcgtt ggcgcggcgc caggaacggg agctgcggca gtggctgtac
    24121 gccgactcgt ccggacgcgg gacgctggtc gaggcgatct cgggcgagat catcgacgtg
    24181 gaggaccgcc acggcgtcga ccttgagctg gtcagcgtcg gcgacaccga gctgactccg
    24241 gagctgaccg cgctggtgaa ggctgcgcgc gaggcgatgg tgaacgcggc caagcattcc
    24301 ggcgcgaccc gcgtcgacgt ctatgtggag gtcgatcccg acgtggtgtc ggtttacgtg
    24361 cgcgaccggg ggaccggctt cgacctggcg cacatggaca ccgaccgcat gggcgtccgc
    24421 ggctcgatcg tcgagcgggt gaagcgggcc ggcggacggg cgataatcag gactgcgccc
    24481 gatgagggca ccgaagtgag actggagctg ccgcgatgac tgagaccgag cctgaagccg
    24541 tccccaccgg cccgtggcgg gtggtgatcg tcgacgacca cgccatgttc cgtagcggcg
    24601 tgaagcacga catcggcgcg cggatcgagc tggtcggcga aggcgaggac gtgcctaccg
    24661 cggtgcgggc catcctggcc accctgccgg acgtggtgct gctggacgtg cacctgcccg
    24721 gcggcggtgg cgcggaggtg atcaagcagg tcacggcgac ccagcccggc gtgaagttcc
    24781 ttgcgctgtc ggtgtcggac gctgccgagg acgtgatcgg ggtgatccgg gcgggggcca
    24841 ggggttatgt gaccaagtcg atctccgccg aggagttggt ggccnnnnnn nnnnnnnnnn
    24901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    24961 nnnnnnnnnn nnnnnnnnnn nnnncgcgag cgcgaggtcc tgcgcctgat cgcccgcggc
    25021 tacgcctaca aggaagtcgc caaggagctc ttcatctcga tcaagaccgt cgagacccat
    25081 gtctcctcgg tgctgcgcaa gctgcagctc tcgaatcgcc atcagctcac ccggtgggcc
    25141 accgaccgca agctggtcta gctgctgtcg ttccccgggc ttgtccggga ctgtcggtgc
    25201 gagcgcttag gcttgtctgg atatgactga cacgctgttc ccggacctgt tcgctgcccc
    25261 cgtccaaccg tccccgctgg ttgcggacgc acccaagcgc gcccggcgga tgagcgagga
    25321 cgagctgttg gcgaacctca atccgccgca gcgggaggcg gtcgtgcatg ccggcgggcc
    25381 ggtgctagtg gtggccggcg cgggctcggg caagacccgg gtcctcaccc ggcggatcgc
    25441 ccacctggtc agccagcgca acgtccatcc cgggtcgatc ctggccatca ccttcaccaa
    25501 caaggcggcc gcggagatgc ggcaccgcgt ggtcgacctg gtcggcaatc gcgccaagct
    25561 gatgtgggtt tcgacattcc actcggcgtg cgtgcgcatt ctgcgctccg acatcggacg
    25621 gttcggcatg agccgcacct tctcgatcta cgacgacacc gacgccaagc ggctgatgac
    25681 cctggtgttg cgcgaccagg agctcgaccc gaagcggtac cccgtccgcg cggtcatgaa
    25741 ctgggtctcg aaccagaaga acgagctggt cgacttcgaa tcggcgcgca cccgtgcggt
    25801 cgagggcacc gccgagcacc actacgccga ggcgtacgcg gagtaccagc ggcggctgaa
    25861 agcggccaac gcgctcgact tcgacgacct gatcatgacc accgtccacc tgctgcaggc
    25921 gttcggtgac ctgcgcgagc agtaccggcg ccggttccgg cacgtactcg tggacgagta
    25981 ccaggacacc aaccacgccc agtacgccct ggttcgtgag ctctgcgctg cgcttcccga
    26041 aggtggggaa gggccgagca tcgacccgcc cgagctgatg gtggtcggcg actccgacca
    26101 gtccatctac gcgttccgcg gtgcgacgat tcgcaacatt ctcgacttcg aagccgactt
    26161 cccgggcgcg accacgatcg tcctcgagca gaactaccgc tccacccaga acatcctgag
    26221 cgcggcgaac gcggtgatca cccgcaatcc cggacgcccg gagaagcggc tctggtcgga
    26281 gaccggcgac ggtgagctgc tggtcggcta cgtcgccgac accgagcacg acgaggcgca
    26341 gttcgttgcc gaggagatcg atcggctgac cgatgccggc acgtccaagc cgggcgacgt
    26401 ggccgtcttc taccggacga acgcgcaatc gcgggccttt gaagaagtgt tcatccgggt
    26461 cggcatgccg taccgggtgg tcggcggcgt ccgcttctac gagcgcaagg agattcgtga
    26521 cgccatctcg tacctgcggg ccatcgctaa ccccgacgac gatgtctccg tccgccgcat
    26581 cctgaacgtg ccgcgccgcg ggatcggcga tcgtgcggag gcgatggtcg aggcgtttgc
    26641 cgctgccgag cagatctcgt tcggcgctgc gctggatcgc gccgcggagg cgcccggatt
    26701 ggcctcgcgc tcgctgagcc agatccaggg cttcgcagcc atgctggccg gcttccgagg
    26761 caaagtcgcg gccggggcgc cggcggacga gatcctggcc ggtgtgctga aggagtcggg
    26821 ctatctggcc gagctcaccg cgtccaccga tccccaggac gagtccaggg tggagaacct
    26881 ggtggagttg gtcagcgtgg cgcaggagtt cgtggctgcg gcgcatgcgg tcgacgtgcc
    26941 cgacgacgag agcgaactgg cggctggcgc accggagccg gacgattcgc tgcccgcttt
    27001 cctcgagcgg atcgcgctcg tggccgactc cgaccagatt ccggacaacg atccggacgc
    27061 gaccggcgtg gtcaccctga tgacgttgca cacagccaag ggcctggagt tcaacaccgt
    27121 gttcctcacc ggcttcgagg acggcatctt tccgcaccag cgagcgatga ccgacgcgaa
    27181 ggagttggag gaggaacggc ggctggccta tgtgggcatc acccgggcgc ggcagcggct
    27241 gtacctgacc cgggccagcg tgcggacgca gtggggcgcg ccgcagtaca acccggccag
    27301 ccggttcacc gacgagatcc ccggccactt ggtcgactgg cggcggcttg gctacgcgcg
    27361 caccgggttc gcgtccgcgt cttcggcgcg gaccgagcag gcgtggcggt cgacggtcgg
    27421 cttcggtgcc aagccggcga tcaagacggt cccttccgtc gcgatcggcg accgggtact
    27481 gcacaccacc ttcggcatgg gcacggtgat cgccacctca ggcatcggcg acaccgccaa
//
LOCUS       DAOPRQ010000035        27271 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_1412_length_27271_cov_12.771458, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000035 DAOPRQ010000000
VERSION     DAOPRQ010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 27271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27271
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1412_length_27271_cov_12.771458"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="PKJ61_06310"
     CDS             <1..217
                     /locus_tag="PKJ61_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13472.1"
                     /translation="DSGLCHQRHPVGDDPVAAFSDVLVDQRRLRRECGAVSTSSISRD
                     EPISCDVPICRDGPTTGSSDSGESSTN"
     gene            499..1005
                     /locus_tag="PKJ61_06315"
     CDS             499..1005
                     /locus_tag="PKJ61_06315"
                     /inference="COORDINATES: protein motif:HMM:NF021075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwaF family protein"
                     /protein_id="HOC13473.1"
                     /translation="MDGRLPLRAGGWGALDTDLFPDWASRPILNIFTWQSFTIHALIL
                     GYVLMRIVAGDLVPDAGNLWRVAVITIAGALLGHLANQAWGTNFWFLDVGAPDSPLEA
                     IQYVAGPFHVPGADRGHARRLDSPPPALDAPRTRPAPVTLRLDSRSYSVSISPNDDFC
                     AATDPSTS"
     gene            1185..2186
                     /locus_tag="PKJ61_06320"
     CDS             1185..2186
                     /locus_tag="PKJ61_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008356850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOC13474.1"
                     /translation="MSQASAVGGRDGAVDQVAGAVAEIAGRTPWQITRARLAKDKVTM
                     VAMGAALFFIVLALLAPVLTHFGLLDPYSNHTDLVGGLGSLPKGPLGGVSPEHWMGVE
                     PGTGRDIFSRVVSGLTVSLIVATLAVTVSVILGTVIGIVSGTAGGRVDWLFSRITDFV
                     LSFPQTLMLIALSTIVIDMLQSALNMERTVPSMIFMILVMGFFGWPIFARVIRGQVLS
                     LKQREFIEAAHSLGAGRARIYFKELLPHLWAPILVYTTLVMPQNIATEAALGFLGVGI
                     QAPTPSFGSILNDSVHYATSDPAYFIFPGVTLFLVVLSFNLLGDGLRDALDPKADRQ"
     gene            2233..3975
                     /locus_tag="PKJ61_06325"
     CDS             2233..3975
                     /locus_tag="PKJ61_06325"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOC13475.1"
                     /translation="MKRTQIVAGVATTLALGLALTACGSSPTATSTAGGATSGAAAGG
                     AAGGTLHILSGTATEHWDPQRVYVGVNIEYGNRMFTRTLTTFGPVAKDGEPATLIADA
                     ATDTGTVSADGKEWKFTIKDGVKWEDGQAVKCEDFKYGISRTFAVDVITGGPNYAIQY
                     LDIPKNDDGSSKYAGPYKKTGQDLYDKAVTCDGNTITFKMAKPSGDFNMAATMTAFGA
                     FREDKDQGEKSNYAIFSNGPYKLEGSWTANKGATFVRNENWDPATDGFRKAYPDKIVW
                     DESLTQETMYERIIADQGDDKNAIAMDQAPVTAMANVTSAEGRYTVVASPYTRYMVPN
                     YKSKVFSNDKAREALALATDRSAYVTAAGGDQVVKATNSLINPALPAFPNTPLLAGEK
                     GDAAKAKEALQASGLTLPVKINVAYRKNDTLDKAFAGLKAGWDAAGFQTNLIGIPAEQ
                     YYGTLQSPESATKYDAYWAGWGADFPSASTVIPQLLDSRSQISAGGPGQDYGYFDNDE
                     FNAGIDKAMALTGAEGDKAWGALDTEVVTKHFGVIPLINDQFVFAHGSNVEGAVVNTT
                     FTGYYDIANVSVKK"
     gene            4033..5049
                     /locus_tag="PKJ61_06330"
     CDS             4033..5049
                     /locus_tag="PKJ61_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOC13476.1"
                     /translation="MFYYILRRLASAAAMIVLITATTFAIFFASPIDPARYTCGKNCT
                     PTILEGNRKALGYDQPATVQYVKFISGLFVGRDFPDNEQLRTEHPEQIVHCPAPCLGY
                     SANQHRTINEMVAEGWPISLSVAIGAFTLWMLIGVGGGLIAALNKGRWPDRTIVGSAL
                     IGFSLPTFFVGLVLLTFPAIKWGWVPIPNFVPFFENPWEWARNLILPWITLAFFFAAS
                     YVRLTRAYMIETMSEDYIRTARAKGVATTAVVMRHGLRAALTPIVTAAGLDLGGLLGG
                     AIITESVFSLHGLGYLAVRSVTNMDLPTIVAMVMIVATFIVIANLIVDVAYSFIDPRV
                     KLVS"
     gene            5046..6068
                     /locus_tag="PKJ61_06335"
     CDS             5046..6068
                     /locus_tag="PKJ61_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008356847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOC13477.1"
                     /translation="MTAKFLRVNDLRVQFPTDDGVVKAVDGLTFDLEKGKTLGIVGES
                     GSGKSVTSLAIMGLHRGSSAKLTGEIWLDDEDLLKVSHEELRRLRGRKMAMIFQDPLS
                     AMHPYYTVGTQLAEALLVHEKVSKKAARATVIDMLGRVGIPNPGRRVDQYPHEFSGGM
                     RQRAMIAMALINNPELLIADEPTTALDVTVQAQILDLMRDLQAEFGSAIIMITHDLGV
                     VAEIADDVLVMYGGKCVEYAEVDDAFHQPDHPYTWGLLTSMPRLDRVKSERLVPVAGN
                     PPSLINIPPGCAFHPRCAFREFAPCPCDEVVPELLESYPKHDSRCHIPVATRAEVFAE
                     RIAPRL"
     gene            6077..7057
                     /locus_tag="PKJ61_06340"
     CDS             6077..7057
                     /locus_tag="PKJ61_06340"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020385614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HOC13478.1"
                     /translation="MSLLEVTDLKKHFPTYQQKLIRTEGDPVKAVDGLTLSLDAGETL
                     GLVGESGCGKSTAGRTILRLLDPTGGKIVFEGQDVTHAEGQGLRNLRRDMQMVFQDPY
                     GSLNPRHPVGAIIAAPFQIQGVKPEGGVKRAVQELMERVGLNPEHYNRYPHEFSGGQR
                     QRIGVARAIALKPKLIVCDEPVSALDVSIQAQVINLLEDIQAEQNLAYVFIAHDLSVV
                     RHISDRVLVMYLGRPMEMADRDALYERPLHPYTQALMSAVPVPEPRRDRERILLKGDL
                     PSPQNPPSGCVFRTRCPYATEKCAAEVPMLVEREYGHQVACHYPAVRQVL"
     gene            complement(7302..8246)
                     /locus_tag="PKJ61_06345"
     CDS             complement(7302..8246)
                     /locus_tag="PKJ61_06345"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOC13479.1"
                     /translation="MLAVCPNPTIDRLVLLATLTPGVVDRVQGNQAYPAGKSVSAARG
                     CRANEAEPRVHVLLPTDGSGWYLDTLRAEGMDVTAHPYPGQVRESIILIEDSGRVTVL
                     NGPGAPVDATRWEEFADAVCAVMQPQEWVICSGSFPPGVGPKALAGFVAAVADAGGRL
                     ALDTGPEWMRAALGGASRPALITPNLAEAESILAGATAVERTDVGADALDRAELAAVR
                     LRERGIDAVVVTAGAAGLAWASADGAGRLAGLSVAVRNPIGAGDAFMGGLVARLEAGH
                     NFADAVAWGMATSAAAIEQWSPGAADSARVAELRGQLR"
     gene            8589..8966
                     /locus_tag="PKJ61_06350"
     CDS             8589..8966
                     /locus_tag="PKJ61_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13480.1"
                     /translation="MNLLRLVDAVFSLTGNAWYFKQLGYTREQIGYFTEIGWLSAAVW
                     AVAIFTGIAGALFLLARRRLAVPVLLIAAIAQAVLLVVGLTLLSSWTALGPALTTWGV
                     LMLLVVGFAAGCAWRLDRRGRLR"
     gene            complement(8985..9995)
                     /locus_tag="PKJ61_06355"
     CDS             complement(8985..9995)
                     /locus_tag="PKJ61_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016454964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOC13481.1"
                     /translation="MVSGLQVADAVVRYGPVTAVDGVSLDVAPGEVVALLGASGSGKS
                     SLLRAVAGLEPLAGGSVSWDGDDLAEVPVHKRGFVVMFQDGQLFPHLSVAGNVGYGLA
                     KDRDRPTRRSQQAKAERVEELLELVGLGGYGPRPITALSGGQAQRVALARSLAADPKL
                     LLLDEPLSALDRGLREHLVGVLDATLRATGTPALYVTHDQDEAFAIADRIAVLADGHL
                     LQLADPATLWRHPAGREVAGFLGYGPFLDPSAASALGWPGTPDGSVAVGPSGLVSDLS
                     GTEVRVLDAQPRRGGTELTVKLPDGQPASVLTPDITTHGRPPAVLAVRLDPSGCTVLA
                     TT"
     gene            complement(9989..11566)
                     /locus_tag="PKJ61_06360"
     CDS             complement(9989..11566)
                     /locus_tag="PKJ61_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008354966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOC13482.1"
                     /translation="MFFAWPVAALVARGFVDETGFTLAGVQAVLAAPRTWRIVYQTLA
                     QAVVGTVFSLGLGLPGAYLLYRCRFPGRRALRAAVSVPFVLPTVVVGVAFQSLLAPHG
                     LLGFLGLQESFIGIVAALVFFNYSVVVRTVGALWSRLDPRAEEAARTLGASRTRVLAT
                     VTLPALAPAIASAAALVFLFCASAFGVVLVLGGASYGTIETEIWYQTTQLLDLPAAAA
                     LSILQLVIVSASLLVANITRARQERALRLSGNPVDKPLRASDWPAVVVTATVALGLLL
                     LPLGSLVWRSFVTADGLGLGNYQALAGNAGQLTVSVWQALANSLVIAAAATALALVLG
                     LLVSYVLSRRPASAPGRRVLRTLDAIFMLPLGVSAVTVGFGFLITLNRPPLDLRSSWV
                     LIPIAQAMVALPLVVRTLLPTLRAIDPRLHEAAATLGASPARIVATIDLPFALRGLGL
                     ALGFAFATSLGEFGATSFLARPDRPTLPVVIFSLIGRPGAANFGMAMAAAVVLALLTA
                     AVMAVAEWIGPKEVNPW"
     gene            complement(11968..13011)
                     /locus_tag="PKJ61_06365"
     CDS             complement(11968..13011)
                     /locus_tag="PKJ61_06365"
                     /inference="COORDINATES: protein motif:HMM:TIGR01254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA];
                     GO_process: GO:0030974 - thiamine pyrophosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HOC13483.1"
                     /translation="MLTSVAAGVLIAGCAGQPVKPVQPAGTGQATASAPTESKTLTVI
                     THDSFTLDDATIGKFRTESGYDVKFVAPGDAGTVVNQLILTKDAPLGDVVFGVDNTFA
                     GRAIEAGVLAPYAASALPPAAAKLAADDKPSLTPIDYGDVCLNADSAWFKANSLAVPE
                     TLDDLLKPEYKDLLVVSNPASSSPGLAFLAATVGAKGEGYLDYWKALKANGLKVVKGW
                     TEAYTVEFSGSSGKGDRPLVLSYASSPAYEPATVALTQTCFRQVEYAGVISGAQNEVG
                     ARKFIDFLLSDEVQAELPEAMYMHPVKPDVPLPEEWQQHAAEVDAPIEVPVAEISARR
                     DEWIKAWTATVIG"
     regulatory      complement(13077..13181)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            13429..15018
                     /locus_tag="PKJ61_06370"
     CDS             13429..15018
                     /locus_tag="PKJ61_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011758103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HOC13484.1"
                     /translation="MTAQSDAAEKIRAGYTFDTPAIELGVLMVDGAPAPDVKIRLSLS
                     MLNRHGLVAGATGTGKTRTLQLLAEQISAAGVSVFAADIKGDLSGIAIPGEPSEKLLA
                     RTQKIGQDWQPAGCPTEFYALGGQGTGVPLRATVSSFGPILLSKVLGLNETQESSLGL
                     VFHYADSQGLALLDLADLRALLTHLTSDEGKAELKEIGGLSSATVGVILRSLITLQTQ
                     GADEFFGEPEFDTRDLIRTVNGKGVISLLELPNLQDRPQIFSTFLMWLLADLFHDLPE
                     IGDVDVPKLVFFFDEAHLLFNGASKAFLDAIAQTVRLIRSKGVGIFFVTQSPTDVPDA
                     VLAQLGSRVQHQLRAHTPKDAKALKATVETYPNSQYDLAETLQALGIGEAIVTVLNPD
                     GAPTPVAWTRMRAPQASMEPMPAEWLTKGVAASAMMAKYGQAINRESAYELLTRRIEE
                     GAAKAAAEKQAAQDAKAQEAAQREYDAEARRSTRTSTRAPSRSNAKSGVEKVLGSPTF
                     WNQVIRAGSEVVRTMWGTAKR"
     gene            complement(15033..16772)
                     /locus_tag="PKJ61_06375"
     CDS             complement(15033..16772)
                     /locus_tag="PKJ61_06375"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOC13485.1"
                     /translation="MSHNQNSRRALAALLGISVSLGLAACAVPDGPVTPGERPNRPTA
                     SASASVNPETDPAKLTISLSQVPAFRPATADADGWTTNAMTDAAGDFKWRVLDADNQV
                     IQDYQGDQPVAFGDPETYTDVPGVLTFRGNNARNGGAYGTPDVKDKKLQVVWTQDTGE
                     VRGDNSYWPGAGWTGQPLLVNWPEATRKAMGYADEFANDPEFTEVIYPTFTGMIYRLD
                     LATGKQTKPPIDTGCGYKGTGSVDPRGYPLLYAGQGLDDMNGVDCPFQYRVFDLIKNV
                     QVAGWSGKDDGAPRQGWGAFDSSALVNAASDTLFAPAENGLVYKAKLNAKFDAEAKTV
                     SVDPQLTKLQYSTPVSEKYGIESSAVAYRNLMFAQDNDGNLICWDANTLTVVWARALG
                     DDSDASLVLEQTADGGVFLYAGNEVDKRTDPDQFITNLRKINALTGELVWQYDVPAQR
                     SLPNNGGLMATPLLGTGEIADLVLFNVAKTTSEKQGDLIALDRKTGKLAWKRHLKRYS
                     WSSPTQITSSDGHAYVVFGDSGGTLHLIDPNTGEDLNTLSLGKNTEASVSAYGDMLVV
                     ASYAKRIWGIRIS"
     gene            complement(16941..17948)
                     /locus_tag="PKJ61_06380"
     CDS             complement(16941..17948)
                     /locus_tag="PKJ61_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013160625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HOC13486.1"
                     /translation="MSSGLATTYLGLPLSGPLIASASPLTGRIDSLLALEAAGASAVV
                     LPSLFEEDVVAEESELAERLEVGDDFAEFSGSPLPEIEQPELGSGRHRRLVQKAKAQL
                     KIPVIASLNATRPGSWQRYAAELADAGADALELNLYSVAADPLKSAAEVELDHLAAIE
                     AVKIAVDIPLAVKLSPFYSAMSKFASLAVDAGADGLVLFNRFYAPDLDLDRLKLFAKA
                     DLSQPDDQRIALRWIGILRSQLPEVALACTSGVHSGEEIIKALLVGANVACTTSAVLR
                     RGPEAITQMLAEADAWLADHDYLGVDQLRGSMSVGSVGDASAYERAQYQAVLLSGRHV
                     G"
     gene            complement(17945..>18706)
                     /locus_tag="PKJ61_06385"
     CDS             complement(17945..>18706)
                     /locus_tag="PKJ61_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016669495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOC13487.1"
                     /translation="SPSADDPGVRRDAMEGALVATRALLSLADELVDKSVWIVGGDGW
                     AYDIGYGGLDHVLGSGRNVNILVLDTEVYSNTGGQASKSTPRAAMAKFATAGKGTPKK
                     DLGVLAQAYGDVYVAQLAIGANQQQTVRAMLEAQAWPGPSLLIAYSTCIAHGIDMATS
                     MTHQAEAVASGYWPLYRYRPGDGQPLHLDSKAPTLPVADFMSSEARFAMLRRANPSRA
                     EELAQLAQADADVRWHYYSQLAGVERVLPAVKEES"
     assembly_gap    18707..18806
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<18807..20623)
                     /locus_tag="PKJ61_06390"
     CDS             complement(<18807..20623)
                     /locus_tag="PKJ61_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016455741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOC13488.1"
                     /translation="MAPTVIGGRYGLSSKEFTPQLAKAVFDELAKPNPKPRFTVGIND
                     DVTHLSLAPDTDFHVASEHISAVFFGLGSDGTVGAAKNSVKIIGSRPDAHAQGYFVYD
                     SRKSGAVTVSHLRFGSTPIRSTYLIDQADFVAVHQFELLKAMQTVDVAKPGGTLLLNS
                     PHGEQTWEHLPIEVQQLIIDKQLRTYVIDAEAVAKAAGLHRRINTVMQPCFFHLSGVL
                     PTEEAVAAIKASVEETYGRRGRTIVEKNYQAIDAALAALSPLVVPAVASGTEHRLPPL
                     PQAAGDFVRQVTAAMLHGQGDLLPVSALPVDGTWPTGTAKWEKRGLAAELPIWDESLC
                     IDCGKCAITCPHTAIRIKLADPADLADAPTGFKHKEYKDRQLPGRRLIVQVAPDDCTG
                     CGICVDICPARSKSELKHKSLNMAPAREHRDVERESFEFFLGLPEIDRTQVRHDQVKG
                     VAQLQPLFEFSLACSGCGETPYLKTLTQLFGDRMIVANATGCSSIFGGNLPTTPYTKN
                     ADGRGPAWSNSLFEDNAEFGLGMRIAWEKQHAEARRLLAELREVVGPELADAILTADQ
                     SDEAGLAAQRERVVELKELLAAHADPSHPADPSHPADPS"
     assembly_gap    20747..20846
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<20847..21943)
                     /locus_tag="PKJ61_06395"
     CDS             complement(<20847..21943)
                     /locus_tag="PKJ61_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016455741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HOC13489.1"
                     /translation="MAESSEAAPVRATLDGNEAAARVAHALSEVIAIYPITPSSAMGE
                     SADAWSAAGRPNLWGGVPEVVELQSEAGAAGALHGAVTKGTLGTTFTASQGLLLMLPN
                     MYKIAGELTPVVIHVAARTVATHALSIFGDHSDVMSARMTGWAMLSASSVQEAHDFAL
                     VAHAATLRTRVPFVHFFDGFRTSHEENTIQLLGDDVLRALVRETDVIAHRSRGLTPDA
                     PMIRGVAENPDTFFQAAEAANPFHFAVPDVVAEVFGELAEHTGRRYSLVDYHGAPDAE
                     RVVVAMGSSAGCLAETVEELVHRGEKVGMLTVRLYRPFPVAAFLAALPRSVRSIAVLD
                     RTKEPGAPAEPLHADVLVALASAGPRHPGLGP"
     gene            complement(21983..25369)
                     /locus_tag="PKJ61_06400"
     CDS             complement(21983..25369)
                     /locus_tag="PKJ61_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002528388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13490.1"
                     /translation="MASTETAAISLRLAQLGLPLPAEVQDGQAKLVAPIIARQRELSR
                     RLADRLCAADARIQSWLDDYLAETGVEPKLPKRTFVLDEPGLARALSLPYDADEFSSP
                     LLHSYRLANGVLHNPANDRRTTAGVFHIAEGGLPIPDDKIAVPKAVFARLLELAFQPP
                     EVDKILPFTANLENPAACFVSLQLRPLVAPAVPGYVREKRMETRFIVPGGLVANLDFV
                     EGIFGNGGDPYLPENDASLLPETWTGHTGAVILAPHLTKVTKKSLGLPHISEATERQR
                     RDGVAWEHEDDLYNAGQAFKVCARDARGVIVTVIADNYFGYCKKEVKTQISYSANLFG
                     NAEEEHAGGALVFPSYNLGQEYTVTPPGDSEYNLAEVVERNPERFELQPEGHALDRQQ
                     EQIVLVPPNANFSLRSMTVTWTDADGNPGSLKLRADKVYLSPDGYQVRMAQQAADRTQ
                     WSLVGTVPTATSCHKPSTVSGGGKSEISKAITDAFIFGAAYTPDYDADMDHVQAILEH
                     DFSDRFAAPTQNGTDRRPILSDDRSIGSVIKLLTPSEREYSPEYNAWLEQIPQHIKEL
                     VFVVKRFYTPAWGEDWRSHFSVGIINGRRGNNLRLDGDKIMVNMLRVGFDADGSWRLF
                     GLRHDFSPAVKVQTEDDITASVVIGGHMLGLDETRSYKLVENCEELLFQRPDDAIHRG
                     YDKQAELDIASPGTFLSNFQPLTKADADEMRDDAVAFSKFTAPMRNLISDFADKPAEV
                     SPKFFVSSASPRLVDGKPSKNPRYLQKRPDRTNVLGTAVADLASHLVRKLPTDAALPL
                     PVDIVAAGRRNNPPEGPVPPLCAFSPLHYLELPELFAEFISSMTGKSPSTTGAGSEGA
                     MTKGPFNAMPAIVDLNAALLSYALTGYDGWVSCAGFVGPYVRVNHDISMLVPEVFSRM
                     TAAERSSANLLAEGALERIEDFEHEGETVLASRLGYRMTQAFSRKYFGRIFLHPHSVF
                     TDEMLRPELQDADIFAESVATIVATHQRVAQAYFDDGTIELAIPPLKALLEIMAHGTS
                     AEGWDLGSPEFRALFSRDSVLASDWYAARLDAKQTAAAGRASAGLQALQKFITTPGNE
                     EPSERLDVPARIRAAEAEYARFSSPEYRTQIVGTVGRQPL"
     gene            25577..26506
                     /locus_tag="PKJ61_06405"
     CDS             25577..26506
                     /locus_tag="PKJ61_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HOC13491.1"
                     /translation="MISRSAVPSTLSQTAELQAGMLSRAQVLRAGISDRVIARLCRQR
                     WLQVAPGVYSVRDEPSWLGWAWAGLLIGGPGSVLGLAAAGHLNQLMPAPDQLAIWVGE
                     HRQVRTRWPWHFIRGQRIGVGSPPRTDRIRTILDLAAVQPSDALLNLLSEAVGRRGVT
                     PSVVLDALAQLPRHPRRKLLIEVLGDVTAGVRSPLERRFLRDVERAHGLPVAERQESP
                     SGRHQVDAWYREFGVVAELDGRAFHEGRNRFRDFERDNRHALLSLTTLRYGWNDTINN
                     PCSVARQLVAMFRLHGWTRVPKSCPRCANRPWP"
     gene            26573..27193
                     /locus_tag="PKJ61_06410"
     CDS             26573..27193
                     /locus_tag="PKJ61_06410"
                     /inference="COORDINATES: protein motif:HMM:NF025255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4203 domain-containing protein"
                     /protein_id="HOC13492.1"
                     /translation="METLGPAIAEIAIGLAFCFFGYPAARFVLALWGAVLGFILGVAG
                     YAVVVGWLGSNLGTIAPWIAGAIGAVILAGLAFAFYWVSVLVAMGSLGWGLGQVLAAS
                     LNVPTWLAVTIAVVVAAGLVIIGWTLDLPRLLLIVVTAFIGAAGIVTGVQLLLGQLNP
                     WYADLGQSLATGANLVWPVAFVLLGIIGVLVQSRQSSGKTLRAAYH"
BASE COUNT         4456 a         9382 c         8845 g         4388 t
ORIGIN      
        1 cgactccggg ctctgccatc agcgccatcc ggtcggcgat gatccggtcg cggccttcag
       61 cgacgtgctg gtagaccagc gccgccttcg ccgtgagtgc ggcgcggtct cgacaagctc
      121 gatcagccgc gatgagccga tcagctgcga tgtgccgatc tgccgggatg ggccgaccac
      181 cgggagctcg gacagcggtg agagttcgac caactgaagg gcgggcaccg gcttcggggt
      241 gttctcaccg atccactcgg gagtgcttgc gctactggct gccggcatcg ccgcactcgt
      301 gctcccaatc gcagagcgga cgcacccagc cgcacccatg ctgcgacacg caatcggctg
      361 gacggtactc ctgctcgaac tcgttcgccg ggccgcctac ctcgccacag gttcctagag
      421 cgccgagatc ctgcccctgc acgtctgcgc gttcgccacg ctcgccgtcc tcatcgactc
      481 ccgcttcccg aacaatcaat ggacgggcga cttcctctac gcgctggcgg gtggggcgcg
      541 ctggacaccg acctcttccc cgactgggcc agccgtccca tcctcaacat cttcacctgg
      601 cagagcttca ccatccacgc cctgatcctc ggttacgtgc tcatgcgcat cgtcgccggc
      661 gacctggttc ccgacgccgg gaacctgtgg cgggtggccg tcatcaccat cgccggcgcc
      721 ctcctcggcc atctcgccaa ccaggcatgg ggcaccaact tctggttcct cgacgtcggc
      781 gcgcccgatt caccgctgga ggcgatccag tacgtcgccg ggccgttcca tgtccccggt
      841 gctgatcgtg gccatgctcg ccgtctggac tctcctccac ctgccctgga tgctccgcgg
      901 acgcgtcccg caccggtaac gctgcggctc gactcccgca gctacagcgt ttcgatatct
      961 ccgaatgacg acttctgcgc tgccaccgac ccgtccacca gctgaaccga ttcatccgca
     1021 tgctggccac ctccaccgac tcgaacgaga aacgatggtt acagtttcct aaccaagctg
     1081 ccaggtctac tgggtttcac ccgttgccgt gctaggtttt ccaagcccat cattgggcgg
     1141 cgaatcgtga ttgcgccgtc tcggcgagga gaaagtaggg ctggatgtcc caagccagcg
     1201 cggttggtgg acgcgacggt gccgtcgatc aggtcgccgg cgcggtcgcc gagatagccg
     1261 gccgcacgcc gtggcagatc acccgtgcgc gcctcgcgaa ggacaaggtc accatggtgg
     1321 ccatgggggc ggcgctgttc ttcatcgtgc tcgcgctgct cgccccggtg ctcacccatt
     1381 tcggcctgct cgacccctac agcaaccaca ccgacctggt cggcgggctc ggctccctgc
     1441 cgaagggccc gctcggtggc gtctcgcccg agcactggat gggtgtcgag cccggcaccg
     1501 gccgcgacat cttctcccgc gtggtctcgg gcctcaccgt ttcgctgatc gtggccacgc
     1561 tggccgtgac cgtgtcggtg atcctgggca ccgtgatcgg catcgtctcc ggcaccgctg
     1621 gcggacgcgt cgactggctc ttctcgcgga tcaccgactt cgtgctgagc ttcccgcaga
     1681 ctctgatgct gatcgccctc tccaccatcg tgatcgacat gctgcagtcc gcgctgaaca
     1741 tggaacgcac ggtcccgtcc atgatcttca tgatcttggt gatgggcttc ttcggctggc
     1801 cgatcttcgc ccgcgtgatc cgcggccagg tgctctcgct gaagcagcgc gagttcatcg
     1861 aggccgccca ctcgctcggc gccggccgcg cccgcatcta cttcaaggaa ctgctcccgc
     1921 acctgtgggc gccgatcctc gtctacacca cgctggtcat gccgcagaac atcgccaccg
     1981 aagccgcgct cggcttcctc ggcgtcggca tccaggcacc caccccgtcc ttcggctcga
     2041 tcctgaacga ctccgtccac tacgccacgt ccgacccggc ctacttcatc ttcccgggcg
     2101 tcacgctgtt cctggtggtc ctttcgttca acctgctcgg cgacggactg cgggacgccc
     2161 tggatccgaa ggccgaccgg cagtgaaccc caccacagat ttctccctaa caccgaacaa
     2221 aggagagcgc gaatgaagcg cacacaaata gtcgccggtg tggcgacgac cctggcgttg
     2281 ggcctggccc taaccgcctg cgggtccagt cccacggcca cctctaccgc cggcggcgcc
     2341 acttcgggtg cagcagcggg cggggcagcc ggcggcaccc tgcacatctt gtccggcact
     2401 gccaccgaac actgggatcc gcagcgcgtc tacgtgggcg tgaacattga gtacggcaac
     2461 cggatgttca cccgcaccct gacgaccttc ggccccgtgg ccaaggacgg cgagcccgcc
     2521 accctgatcg cggacgcggc caccgacacc ggcaccgtga gcgcggacgg caaggagtgg
     2581 aagttcacca tcaaggatgg cgtcaagtgg gaggacggcc aggccgtcaa gtgcgaggac
     2641 ttcaagtacg gcatctcgcg caccttcgct gtcgacgtga tcaccggtgg cccgaactac
     2701 gccatccagt acctcgacat ccccaagaac gacgatggct cctcgaagta cgccggcccg
     2761 tacaagaaga ccggccagga cctctacgac aaggccgtca cctgtgacgg gaacaccatc
     2821 accttcaaga tggccaagcc gtcgggcgac ttcaacatgg ccgccacgat gaccgccttc
     2881 ggcgccttcc gcgaggacaa ggaccagggc gagaagtcca actacgccat cttctccaac
     2941 ggtccttaca agctcgaggg cagctggacg gccaacaagg gcgcgacctt cgtccgcaac
     3001 gagaactggg atccggccac ggacggcttc cgcaaggcgt acccggacaa gatcgtctgg
     3061 gacgagtcgc tgacccagga gaccatgtac gagcgcatca tcgccgatca gggtgatgac
     3121 aagaacgcga tcgccatgga ccaggccccg gtgaccgcca tggccaacgt gaccagcgcc
     3181 gaaggccgct acaccgtcgt ggcctcgccg tacacccgct acatggtgcc gaactacaag
     3241 tccaaggtgt tcagcaatga caaggcccgc gaggctctgg ctctggccac cgaccgcagc
     3301 gcctacgtca ccgcagccgg tggcgatcag gtcgtgaagg cgaccaactc gctgatcaac
     3361 ccggccctgc cggcgttccc gaacaccccg ctgctggcgg gcgaaaaggg cgacgcggcc
     3421 aaggccaagg aggctctgca ggcgtccggt ctcaccctcc cggtgaagat caacgtcgcg
     3481 taccgcaaga acgacaccct cgacaaggcg ttcgccgggt tgaaggccgg ttgggacgcg
     3541 gctggcttcc agaccaacct gatcggcatt ccggccgagc agtactacgg caccctgcag
     3601 tcgccggaat ccgccaccaa gtacgacgcg tactgggccg gctggggcgc ggacttcccg
     3661 tccgcctcca cggtgatccc gcagctgctg gattcccgca gccagatcag cgccggtggc
     3721 cccggtcagg actacggcta cttcgataac gacgagttca acgccggtat cgacaaggcc
     3781 atggccctca ccggtgcgga aggcgacaag gcctggggcg ccctcgacac cgaggtcgtg
     3841 accaagcact tcggcgtcat cccgctgatc aacgaccagt tcgtgttcgc tcacggcagc
     3901 aatgtcgaag gggccgtcgt gaacaccacg ttcaccggct actacgacat cgctaacgtc
     3961 tccgtcaaga agtgatcaaa gggcttgggg ggcgcctttc ggcgcccccc aagctccccc
     4021 aataggagcg ctgtgttcta ctacatcctc aggcggctgg cctcggccgc ggcgatgatc
     4081 gtcctgatca ccgcgaccac tttcgcgatc ttcttcgcct cgccgatcga ccccgctcgc
     4141 tacacctgcg gtaagaactg cactccgacc atcctcgaag gcaatcgcaa ggcgctgggc
     4201 tacgaccagc cagccaccgt ccagtacgtc aagttcatct ccggcctgtt cgtgggtcgt
     4261 gacttccccg acaacgaaca gctccgcacc gagcaccctg agcagatcgt ccactgcccc
     4321 gccccgtgcc tcggctactc ggccaaccag caccgcacca tcaacgagat ggtcgccgaa
     4381 ggctggccga tctcactctc ggtcgccatc ggtgcgttca ccttgtggat gctgatcggc
     4441 gtcggcggcg gcctgatcgc agcgctcaac aagggccgat ggcccgaccg gacgatcgtc
     4501 ggctccgccc tgatcggctt ctcgctgccc accttcttcg tcggcctggt gctactcacc
     4561 ttcccggcca tcaaatgggg ctgggtcccg attcccaact tcgtgccgtt cttcgagaac
     4621 ccgtgggaat gggcccgaaa cctgatcctg ccgtggatca ccctcgcctt cttcttcgcc
     4681 gccagctacg tgcggctgac cagggcatac atgatcgaaa ccatgagcga ggactacatc
     4741 cgcaccgcgc gcgccaaggg cgtcgccacc accgccgtgg tgatgcgaca cgggctgcgc
     4801 gcagcactca ccccgatcgt caccgccgcc ggcctcgacc tgggcggcct gctgggcggt
     4861 gcgatcatca cagaaagcgt gttttccttg catgggctcg gctacctcgc cgtccgttcg
     4921 gtgaccaata tggacctgcc gaccatcgtg gccatggtga tgatcgtggc gaccttcatc
     4981 gtgatcgcga acctgatcgt ggacgtcgcc tacagcttca tcgacccgag agtgaagctc
     5041 gtctcatgac cgccaagttc ctgcgcgtga acgacctacg ggtgcagttc cctaccgacg
     5101 acggcgtcgt gaaggccgtg gacgggttga cgttcgatct ggaaaagggc aaaaccctcg
     5161 gtatcgtcgg cgagtccggt tcgggcaagt cggtgacatc gctggcgatc atgggcctgc
     5221 accgcggttc gagcgccaaa ctcaccggcg agatctggct ggatgatgag gacctgctga
     5281 aggtctcgca cgaggagctc cgccggctgc gcgggcgcaa gatggcgatg atcttccagg
     5341 acccgctgtc ggccatgcac ccctactaca cggtcggcac ccagttggcc gaagcgctgc
     5401 tggtgcacga gaaggtatcc aagaaggccg ctcgcgccac cgtgatcgac atgctcgggc
     5461 gagttggcat cccgaatccg ggacgccgcg tcgaccagta cccgcacgag ttctccggcg
     5521 gcatgcggca gcgcgcgatg atcgcgatgg cgctgatcaa caaccctgag ctgctgatcg
     5581 ccgacgagcc caccacggcc ctggacgtga ccgtgcaggc ccagatcctc gacttgatgc
     5641 gcgacctgca ggccgaattc ggctccgcga tcatcatgat cacccacgac ctgggcgtgg
     5701 tggctgagat cgccgacgac gtcctggtga tgtacggcgg caagtgcgtg gagtacgccg
     5761 aggtggacga cgccttccac cagcccgatc acccctacac ctggggcctg ctcacctcga
     5821 tgccccggct cgaccgggtc aagtccgagc gcctggtgcc ggtggccggc aacccaccgt
     5881 cgctgatcaa cattccgccg ggctgcgcct tccatccacg ctgcgccttc cgcgagttcg
     5941 cgccatgccc ctgcgacgag gtcgtgcccg agctgctcga gtcgtacccc aagcatgatt
     6001 cgcggtgcca catcccggtc gccacgcgcg ccgaggtctt cgccgaacgc atcgccccca
     6061 ggttgtagga gtagctgtga gcctgcttga agtcaccgac ctgaagaagc acttccctac
     6121 ctatcagcag aagctgatcc gcactgaagg cgacccggtg aaggctgtgg acggcctcac
     6181 cctcagcctc gacgcgggcg agacgctcgg tctggtgggc gagtccggct gcggcaagtc
     6241 gaccgcggga cgcaccatcc tgcggctgct cgacccgacc ggcggcaaga tcgtcttcga
     6301 gggccaggac gtcacccacg ccgagggcca agggttgcgc aacctgcgcc gagacatgca
     6361 gatggttttc caggatccgt acggatcgct gaacccaagg catccggtgg gtgcgatcat
     6421 cgccgcaccg ttccagatcc agggcgtgaa gcccgagggc ggggtgaagc gagccgtcca
     6481 ggagttgatg gagcgggtcg gcctgaaccc ggagcattac aaccgctacc cgcacgagtt
     6541 ctccggcggc cagcgccagc gcatcggcgt ggcccgggcg atcgccctaa agccgaagct
     6601 gattgtctgc gacgagccgg tctcagcgct ggacgtgtcc atccaggcgc aggtgatcaa
     6661 cctgttggag gacatccagg ccgagcagaa cctggcctac gtgttcatcg cgcacgacct
     6721 gagcgtggtg cggcacatct ccgaccgggt cttggtgatg tacctcggcc ggccgatgga
     6781 gatggctgac cgggacgcgc tgtacgagcg tccgctgcat ccctacacgc aagcgctgat
     6841 gtcggcggtg ccggtgcccg agccgcgtcg ggaccgcgag cggatcctgc tcaagggcga
     6901 cctgcccagc ccgcagaacc cgccgtccgg ctgcgtcttc cgaacccgct gcccgtacgc
     6961 caccgagaag tgcgcggccg aggtgcccat gctggtcgag cgcgagtatg gccaccaggt
     7021 cgcctgccac taccccgccg tccgccaggt gctgtaggag ggttccgacc tgcttttagc
     7081 gggccattta gccgctgctg cggtccttgc cgggccaaac ccgagttcga ttggttatcg
     7141 ggcaggaatt cggcttcaaa acccatccaa ctcaggttgc gcccttccac tcccttggcg
     7201 agggctccat aaccacccga aaccgggttg aaccgaccta gatgcggctg gcgagccgat
     7261 gtatccggcg atggcacccg ccgccgcgat ctttgcggcc gtcacctgag ctggccgcgc
     7321 aactccgcga cgcgggccga gtccgcagca cccggcgacc actgctcgat agctgcggcc
     7381 gacgtggcca tgccccacgc gacagcgtcg gcgaagttgt gtccggcctc cagccgggct
     7441 accagtccac ccatgaaagc gtcaccggca ccgatcgggt tgcgcaccgc caccgacagt
     7501 ccggccagtc ggccggcccc gtccgcggac gcccacgcca gccccgccgc cccggcggtg
     7561 accacgaccg catcgatccc gcgctcacgc agccggacgg cggccagttc ggcccggtca
     7621 agcgcatcgg cgccgacgtc cgtccgctcg accgcagtgg cgccggcgag gatcgactcc
     7681 gcctcggcca ggttgggcgt gatcagggcc ggacgcgacg ctccgcccag tgcggctcgc
     7741 atccattccg gcccggtgtc cagcgcaagt cgaccaccgg cgtccgccac tgcggcgacg
     7801 aagccagcca gcgccttcgg cccgacaccc ggcgggaagc ttcccgagca gatcacccac
     7861 tcctgcggct gcatcactgc gcacaccgcg tccgcgaact cctcccagcg ggttgcgtcc
     7921 accggggcgc cgggaccgtt cagcacggtc acgcggccgc tgtcctcgat caggatgatc
     7981 gactcccgca cctggcctgg atacgggtgc gcggtcacgt ccatgccctc ggcgcgaagc
     8041 gtgtccagat accagcccga gccgtccgtc ggcagcagga cgtgcacgcg cggctcagct
     8101 tcgttggccc ggcagccgcg cgccgcagag accgacttgc cggctggata cgcctgattg
     8161 ccctggacgc ggtcgaccac acccggggtg agtgtcgcga gcagcaccag ccggtcgatg
     8221 gtcggattgg ggcagaccgc cagcatgtga cctcctcttc gcaacccgag tccagcagtt
     8281 tgccggcagc ctcgcaagcg gatccctgct gctgtgagca gaccggcgcc gggacgacca
     8341 cgcggggttc agattcagga actcggctca gcgcaatggt gaatcctctg gtacccgaag
     8401 acggcggagt ttgtcttcgg gagattcgca gcgactaccg ccggaggtgg gcggagtagg
     8461 ctgtgctcga ctcctggagg tacccgatga ccgagcccag ctctgccgca agcgccacgc
     8521 cgactccggc acgggcgatc gaccgcccgc cctggcagac gtgggtgatt ggccttgtcg
     8581 tcttggtggt gaatctgctc agactcgtgg acgcggtgtt cagcctcacc ggcaacgcgt
     8641 ggtacttcaa gcagctcggg tacacccgcg agcagatcgg ctacttcacc gagattggct
     8701 ggctatcggc tgcagtctgg gcggtcgcca tcttcaccgg catcgccgga gccctgttcc
     8761 tgctggcccg cagacgcctc gctgttccag tgctgctgat cgccgcgatc gcccaggctg
     8821 tgctcctggt ggtcggcctc acccttctga gcagctggac cgcactcggc ccggccctca
     8881 cgacctgggg agtgctaatg ctgctggtag tcgggttcgc agccggctgc gcctggcggc
     8941 tggaccggcg cggccgcctg cgctgaacca gcccgatccg aggctcaggt cgtcgccagc
     9001 acggtgcagc cggacgggtc gagccgcacc gccagcaccg ccggcggtcg gccgtgggtg
     9061 gtgatgtccg gggtgaggac gctcgccggc tggccgtcag ggagcttcac ggtcagctcg
     9121 gtgccgccgc gacgcggttg cgcgtccagc accctgacct cggtgccgct cagatcggac
     9181 accaggccgg acggcccgac cgcgaccgac ccgtccggcg tacccggcca gcccagggcg
     9241 gacgccgccg acgggtcaag gaacggcccg tagccgagga agccggccac ttcccgtccg
     9301 gccggatgac gccacagtgt cgccggatcg gcgagctgga gcaggtggcc atccgcgagc
     9361 actgcgatcc gatcggcgat cgcgaaggcc tcatcctggt cgtgggtaac gtagagcgcc
     9421 ggcgtcccag tggcgcgcag cgttgcgtcc agcacgccga ccaggtgctc gcgcaagccc
     9481 cgatcgagcg ccgacagcgg ctcgtcgagc agcagcagct tgggatcggc ggccagcgag
     9541 cgggccagtg ccacccgctg ggcctggcct ccggagagcg cggtgattgg acggggcccg
     9601 tacccgccca ggcccaccag ttcgagcagc tcctctactc gctcggcctt cgcctgctgg
     9661 ctccgtcgcg tcggccggtc tcggtccttc gccagcccgt acccgacatt cccggccacc
     9721 gacagatgcg ggaagagctg cccgtcctgg aacatcacca cgaatccgcg cttgtgcact
     9781 ggcacttcgg cgagatcgtc gccgtcccag gagaccgagc cgccggccag tggctccaat
     9841 ccggcgactg cgcgcaacag cgacgactta cccgatccgg acgcgcccag caatgccacc
     9901 acttcacccg gagcgacgtc cagcgagacg ccgtcgaccg ccgtgaccgg gccgtagcgg
     9961 accactgcgt ccgccacctg cagaccactc accatgggtt gacctccttg ggcccgatcc
    10021 attccgcgac ggccatgacg gccgcggtca gcagcgccag caccaccgct gccgccatgg
    10081 ccatcccgaa gttggccgcg ccggggcggc cgatcaggct gaagatcacc accggcagcg
    10141 ttggccggtc cggacgcgcg aggaatgagg tggcaccgaa ctcgcccagc gaagtggcga
    10201 atgcgaagcc gagcgccaga ccgagcccgc gcagcgcgaa cggcaggtcg atggtggcca
    10261 cgattcgggc tgggctggcg ccgagggttg ccgcggcctc gtgcagtcgc gggtcgatcg
    10321 cgcggagcgt gggcagcagc gtccgcacca ccagcggtag cgcgaccatc gcctgtgcga
    10381 tcgggatcag cacccagctc gaacgcagat cgagcggtgg acggttcagc gtgatcagga
    10441 agccgaagcc gacagtcacc gccgaaaccc ctagtggcag catgaagatc gcatccagcg
    10501 tgcgtagcac tcgacgcccg ggtgcagatg ccggtcggcg cgaaagcaca tagctgacca
    10561 gtagtccgag cactagggcg agcgcggtcg ccgctgcggc gatgaccagc gaattggcga
    10621 gcgcctgcca cacgctgacg gtcagctgac cggcattgcc ggcaagagcc tggtagttgc
    10681 cgagccccag tccgtccgcg gtgacgaagg atcgccagac cagcgatccc agcggcaaca
    10741 gcaagaggcc aagcgcgacc gtggcggtga ccacgaccgc cggccagtcg gacgcacgca
    10801 acggcttgtc gaccgggttg ccggagagcc gcagcgcccg ttcctggcga gcgcgggtga
    10861 tgttcgcgac caggagtgat gcgctcacga tcaccagttg caggatcgac agcgctgcgg
    10921 cggcgggcag gtcgagcagc tgggtggtct ggtaccagat ctcggtttcg atggtgccgt
    10981 aggacgctcc gccgagcacc agcaccaccc cgaacgcgga ggcacagaac aggaagacca
    11041 gcgcagcagc ggacgcgatc gccggcgcga gggcgggcag ggtgacggtg gcgagcaccc
    11101 gcgtccggct ggcgccgagg gtgcgcgcgg cctcctcagc ccgggggtcg agccgcgacc
    11161 agagcgcacc caccgtccgc accaccacgg agtagttgaa gaacaccagc gcggccacga
    11221 ttccgatgaa cgactcctgc aggccgagga agccgagcag tccgtgcggg gccagcagcg
    11281 attggaaggc cacacccact acgacggtcg gcagcacgaa cgggacgctg accgccgctc
    11341 gcaacgctcg gcgtccgggg aagcggcaac ggtagagcag gtaggcgccg ggcagcccca
    11401 gccctagtga gaagacggtt ccgacgaccg cctgggcgag ggtctggtag acgatccgcc
    11461 aggttcttgg ggccgcgagg acggcttgga cgccagcgag ggtgaagccg gtctcgtcaa
    11521 caaacccacg cgcgaccagg gcagccaccg gccacgcgaa gaacaccgcc aggaaagcgg
    11581 ccggaaggac cgccgcggcc gtccagaccc aggtgtgccc gccaaggccc gtagcgcccg
    11641 tccgcccgaa agcccccgtt cgcgcggaac ccgacccgac gccccgccgc gacccggctc
    11701 ccggcccgac tccggctccc ggcccgccgg ccagccccga ccccaccccc ggcccggact
    11761 cggctcccgg cccggactcg ggtccccgcc ccgactccgc ggctttgctc ccgaaaggga
    11821 ccctcgctcc cgaattatcg ggagcgaggg tccctttcgg gagcgaacct tggacgggcg
    11881 ggagcggact ctggacgggc gggagcgaac tctgtgccga cggacgcgag tccgagccct
    11941 gcggacgcga gtccggcgcc ggctcggtca cccgatgact gtcgccgtcc atgctttgat
    12001 ccattcgtcc cgcctggcgg aaatctccgc gaccggcacc tcgatgggtg cgtccacctc
    12061 cgcggcgtgc tgctgccatt cctcgggcag tgggacgtcg ggcttgaccg ggtgcatgta
    12121 catcgcttcg ggcagttcgg cctgcacttc gtcggacagc aggaagtcga tgaacttgcg
    12181 ggcgcccacc tcgttctgtg cacccgagat cacgcccgcg tactcgacct ggcggaagca
    12241 ggtctgggtg agcgcgaccg tggccggctc ataggccggt gaagaggcgt acgagagcac
    12301 cagcggacgg tcgcccttgc ccgacgaccc ggagaactcg acggtgtagg cctccgtcca
    12361 gcccttgact accttcagcc cgttggcctt cagcgccttc cagtagtcca ggtagccctc
    12421 gcccttggcg ccgacggtcg cggccaggaa ggccagacca ggcgaagaag atgccgggtt
    12481 cgagaccacg agcaggtcct tgtactcggg cttcagcagg tcgtccaggg tttccggcac
    12541 ggccagcgag ttagccttga accaggcgga gtcggcgttc aggcagacat cgccgtagtc
    12601 gattggggtc agcgacggtt tgtcgtccgc tgccagcttg gctgcagcgg gaggcaacgc
    12661 ggacgcggcg tagggcgcca gcacgcccgc ttcgatagcg cgcccggcga aggtgttgtc
    12721 gacgccgaac acgacatcac caagcggagc gtccttggtg aggatgagct gattcaccac
    12781 cgtcccggca tctccgggcg ccacgaactt cacgtcgtag ccggattcgg tcctgaactt
    12841 gccgatggtc gcgtcgtcga gcgtgaacga gtcgtgagtg atcacggtga gcgtcttgct
    12901 ctcagtgggc gcggacgcgg tcgcctgacc cgtcccggcc ggctggactg gtttcacggg
    12961 ctgcccggcg cacccagcga tcagcactcc ggcagcaacg gacgttagca ccgtagtcag
    13021 aattgaccgg ttgaatagca catttcctcc aatatctcgg agggcccggg ttcccccggg
    13081 gagatttccc gactccctac gccggtgtta accggatcag gttcgagggt ctgcggcagc
    13141 ccgcactctc agcgcccggc ggcgctcccc tgtcttgggt caagggtacc gtgtcgcgat
    13201 ctcgcagccg tgcatgctca gcgcagcggg cgccggttcc ggtacgtcaa ccgccttcga
    13261 attcccgaca ctgcctgagt tgggccacgg acgctcccga cccaggtgac gcgatctgga
    13321 cccgatcggg cctcaccaac cagctcacag gcagaaaccg gtcgtcacgg gtcggaatcg
    13381 cgttaccact cgccccacgc gcgcccggaa atccctagtc ttgggcacat gactgcgcag
    13441 tccgacgccg ccgagaagat cagggccggc tacaccttcg ataccccggc catcgagctc
    13501 ggcgtgttga tggtggacgg ggcacccgcc cccgacgtga agatccggct ctccttgtcg
    13561 atgctgaacc ggcacgggtt ggtggccggc gcgaccggta ccggcaagac ccgcaccctg
    13621 caactgctcg cggagcagat ctccgcagcc ggggtgagcg tgttcgccgc cgacatcaag
    13681 ggcgacctct cgggcatcgc gatcccggga gagccaagcg agaaactcct tgccaggacg
    13741 cagaagatcg gccaggactg gcagcccgcc ggttgcccta ccgagttcta cgcgctgggc
    13801 ggtcagggca cgggtgtgcc gcttcgggcc acggtgtcga gcttcggacc gatcctgctg
    13861 agcaaggtgc tgggtctcaa cgagacccag gaatcctccc tcgggctggt cttccactac
    13921 gccgacagcc agggcctggc gctgctcgac ctcgctgacc tgcgggcgct actgacccac
    13981 ctgacctccg acgagggcaa ggccgagctg aaggagatcg gcgggctgag ctcggccacc
    14041 gtcggcgtga tcctgcgcag cctgatcacc ctgcagacgc agggcgcgga cgagttcttc
    14101 ggcgaacccg agttcgacac ccgcgacctg atccgcaccg tcaacggcaa aggcgtgatc
    14161 tcgcttctcg aactgccgaa cctgcaggac cggccgcaga tcttctccac cttcctgatg
    14221 tggctgctgg ccgacctttt ccacgacctc cccgagatcg gcgatgtgga cgtacccaag
    14281 ctcgtgttct tcttcgatga ggcacacctg ctgttcaacg gcgcgtccaa ggcgttcctg
    14341 gacgcgatcg cgcagaccgt ccgcctcatt cggagcaagg gcgtcggcat cttcttcgtc
    14401 acccagtcgc ccaccgacgt gccggacgcg gtgctggccc agctcggctc ccgggtgcag
    14461 caccagctgc gggcccacac ccccaaggac gccaaggcgc tgaaggccac cgtcgagacc
    14521 tatccgaaca gccagtacga cctggccgag accctgcagg cgctgggcat cggcgaggcg
    14581 atcgtcacgg tgttgaaccc ggacggcgct cccaccccgg tcgcgtggac gcgaatgcgc
    14641 gctccacaag cctcaatgga gccgatgccg gccgagtggc tcaccaaggg cgtggccgcg
    14701 tccgccatga tggcgaaata cggccaggcg atcaaccgcg agtcggccta tgaactgctc
    14761 acccggcgaa tcgaggaagg cgcagccaag gccgccgcgg agaagcaggc agcgcaggac
    14821 gcgaaggccc aggaagcagc gcagcgcgag tacgacgctg aagctcgacg tagtacccgg
    14881 acgagcaccc gggcaccgtc gcgctcaaat gccaagtcgg gtgtggagaa ggtgctgggc
    14941 tcgccgacct tctggaatca ggtaatccgc gccggcagcg aggtcgtccg cacgatgtgg
    15001 ggcaccgcca aacgctgaac gacgcctggc tatcagctga tccggatacc ccagatgcgc
    15061 ttggcgtagg acgccaccac cagcatgtcc ccgtaggccg acactgaagc ctcggtgttc
    15121 ttgcccaagc tgagggtgtt gagatcctcg ccggtgttgg ggtcgatcag atgcagcgtg
    15181 cccccggagt cgccgaagac gacataggca tggccgtcag agctggtgat ctgggtcggc
    15241 gaactccacg agtagcgctt gaggtgccgc ttccaggcca atttgcccgt cttgcgatcg
    15301 agggcgatca ggtcgccctg cttctccgac gtggtcttgg cgacgttgaa cagcaccaga
    15361 tcggcgatct caccggtgcc gagcagcggt gtggccatca ggccgccgtt gttcggcagg
    15421 ctgcgttgag caggaacgtc gtactgccag accaactcgc cggtgagggc attgatcttg
    15481 cgcaggttgg tgatgaactg gtcggggtcg gtgcgcttgt ccacctcgtt gcccgcgtac
    15541 aggaacacgc cgccgtccgc ggtctgctcc aacaccagcg acgcatcgga gtcgtcgccc
    15601 agcgccctcg cccagaccac ggtcagcgtg ttggcgtccc agcagatcag gttgccgtcg
    15661 ttgtcctggg caaacatcag attgcggtag gcgaccgccg aggactcgat gccgtatttc
    15721 tcgctaaccg gggtgctgta ctgcagcttg gtcagttgcg ggtcgacact gaccgtcttg
    15781 gcctcggcgt cgaacttcgc gttcagcttg gccttgtaga ccagaccgtt ctcggccggc
    15841 gcgaagagcg tgtcggaggc tgcgttcacc agcgcagacg agtcgaaggc gccccatcct
    15901 tgacgcggcg cgccatcgtc cttgcccgac catcccgcta cctgcacgtt cttgatcagg
    15961 tcaaagaccc ggtactggaa tgggcaatca acgccgttca tgtcgtcgag gccctggccg
    16021 gcgtacagca gcgggtatcc ccgcggatcg accgagcccg tgcccttgta gccgcagccg
    16081 gtgtcgatcg gcggcttggt ctgcttaccg gtggccaggt cgagccggta gatcatcccg
    16141 gtgaaggttg gatagatcac ctcggtgaat tcagggtcgt tggcgaactc gtcggcgtac
    16201 cccatcgcct tgcgggttgc ctcgggccag ttcaccagca gtggctggcc ggtccagccg
    16261 gcacccggcc aataggagtt gtccccgcgc acctcaccgg tgtcctgggt ccacaccacc
    16321 tgaagcttct tatccttgac gtcaggcgtg ccatacgccc cgccgttgcg cgcgttgttg
    16381 ccgcgaaagg tcagcacacc gggcacatcg gtgtaggtct ccggatcgcc gaaggccacc
    16441 ggctgatcgc cctgatagtc ctggatcacc tggttgtcgg cgtccagcac ccgccacttg
    16501 aagtcgcctg cggcgtcggt catcgcgttg gtggtccagc cgtccgcgtc cgcggtggcc
    16561 ggacggaagg ccggcacctg gctcagcgag atggtcagct tggccgggtc ggtctcggga
    16621 ttgaccgaag cggacgcgga cgccgtcggc cggttgggcc gctctcccgg cgtcaccggc
    16681 ccgtccggca ccgcgcaggc ggccagaccg agggagacgg agatgccgag cagcgcggcc
    16741 agggcgcggc gggagttctg gttgtggctc atcgcgatcg agtctaggcc cgggcgttgg
    16801 tggcccgact caaccccgcc caggtcaccg caaagtggac cgtttggtgg cgtccgggcg
    16861 cgagaccgtc caccttgggg tggggtgact gccggaagac gccccgatca gcaggacaag
    16921 ggaccgttag gggctgaacg tcagccgacg tggcgtccgg agagcagcac ggcctggtac
    16981 tgggcgcgct cgtaggccga cgcatcccct accgaaccca cgctcatcga accgcgcagc
    17041 tggtccaccc cgagatagtc gtggtcagcc agccaggcgt ccgcctcggc cagcatctgg
    17101 gtgatcgcct cgggcccgcg gcggagcacg gccgaagtcg tgcacgccac gttcgcgcca
    17161 accaggagcg ccttgatgat ctcctcaccg gaatgcactc cagacgtgca cgccagcgct
    17221 acttcaggca gctgactacg caggatgccg atccagcgga gcgcgatccg ctggtcgtca
    17281 ggctgcgaga gatcggcctt cgcgaacaac ttgagccggt ccagatccag gtcgggagca
    17341 tagaaacggt tgaacagcac caggccgtcg gcgcccgcgt ccaccgccag cgaagcgaac
    17401 ttcgacattg ccgagtagaa cggcgacaac ttcaccgcga gcgggatgtc gacggcgatc
    17461 ttcaccgctt cgatcgcggc cagatggtcc agctcgacct cagcagccga cttcaacggg
    17521 tcggctgcca ccgagtagag gttgagttcg agggcgtccg cgccagcgtc cgccaactcg
    17581 gcggcgtacc gctgccaaga gccgggccgg gtcgcgttca ggctcgcgat cacggggatc
    17641 ttcagctgcg ccttcgcctt ctgcaccagc cgtcgatgcc gtcccgaacc caactcgggc
    17701 tgctcaatct cgggcagcgg gctgccggag aactcggcga agtcgtcccc cacctccagc
    17761 cgttcggcca gttcggactc ctccgcgacc acgtcctcct cgaacagcga cggcagcacc
    17821 actgcggacg cgcccgcggc ttccagggcc aacaacgagt cgatccgccc ggtcagcggg
    17881 gacgcggacg cgatcagcgg gccggacagc ggcagcccga ggtaggtggt ggccagaccg
    17941 gagctcatga ttcctccttg acggccggca ggacgcgttc cacaccggcc agttgcgagt
    18001 aatagtgcca gcgaacgtcg gcgtccgctt gggctagctg tgccaattcc tcggctcttg
    18061 acggattggc ccgtctaagc attgcgaacc gggcctcgct cgacatgaag tcagccaccg
    18121 ggagggtggg cgccttactg tccagatgca gcggctggcc gtcgccggga cggtagcgat
    18181 acagcggcca atacccgctg gctacggctt ccgcctgatg ggtcatggac gtggccatgt
    18241 cgatgccgtg cgcgatgcac gtcgaatagg cgatcagcag ggacggcccc ggccacgcct
    18301 gggcctcgag catcgcgcgg accgtctgct gctggttcgc tccgatcgcc aactgcgcga
    18361 catagacgtc tccgtaggcc tgcgccaaca cgccgagatc cttcttcggc gtgcccttcc
    18421 ccgccgtcgc gaacttcgcc atcgctgcgc gcggggtcga cttggacgcc tgcccgccgg
    18481 tgttggagta gacctcggtg tcgagcacca ggatgttcac gttccggccg ctaccgagca
    18541 catggtcgag accgccgtag ccgatgtcgt acgcccagcc gtcgccgccg acgatccaga
    18601 ccgacttgtc caccagttcg tcggcgaggc tgagtagcgc ccgggtagcc acgagggcac
    18661 cctccatggc atcccggcga acgccgggat catccgcgga cggagannnn nnnnnnnnnn
    18721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18781 nnnnnnnnnn nnnnnnnnnn nnnnnntgac ttgggtccgc ggggtgactt gggtcagcgg
    18841 gatgacttgg gtcagcgtgt gcggccagga gctccttgag ttccacaacc cgctcgcgct
    18901 gggcggccag gccggcctcg tcggactggt cggcggtcag gatcgcgtcc gccaactcgg
    18961 ggccaaccac ctcgcgcaac tcggccagca agcgccgggc ctcggcgtgc tgcttctccc
    19021 aggcgatccg catcccgagt ccgaactcgg cgttgtcctc gaacagcgag ttcgaccagg
    19081 ccgggccccg accgtccgcg ttcttcgtgt acggggtagt cggcaggttg ccgccgaaaa
    19141 tggacgagca gccggtggcg ttcgccacga tcatccggtc gccgaagagc tgggttagcg
    19201 tcttcaggta cggcgtctcg ccgcagcccg agcaggccag cgagaactcg aacagcggct
    19261 gcagctgggc cacgcccttc acctggtcgt ggcgcacctg cgtccgatcg atctcgggca
    19321 gacccaggaa gaactcgaag ctctcgcgct cgacgtcgcg atgctcgcgc gcgggggcca
    19381 tgttcaacga cttgtgcttg agctcggact tcgagcgcgc cgggcagatg tccacgcaga
    19441 tgccgcaacc ggtgcagtcg tcaggcgcca cctggacgat cagccggcgt ccgggaagct
    19501 ggcggtcctt gtactccttg tgcttgaacc cagtcggagc gtccgcgaga tcggccgggt
    19561 cggccagctt gatccggatc gcagtgtgcg ggcaggtgat cgcacacttg ccacaatcga
    19621 tacacagcga ttcgtcccag atcggcagct cggccgcgag cccgcgcttc tcccacttcg
    19681 cggtgccggt cggccaggtg ccgtccaccg gcaacgcgct caccggcagc aggtcgccct
    19741 ggccgtgcag catcgccgcg gtgacctggc ggacgaagtc acccgccgcc tgtggcagcg
    19801 gcggcagccg gtgttcggtg ccggacgcaa ccgccggcac caccagcggc gaaagggcgg
    19861 ccagcgccgc atcgatggcc tggtagttct tctccacgat cgtgcgtccg cggcggccgt
    19921 aggtctcctc gaccgacgcc ttgatcgcgg ccaccgcctc ctcggtcggc agcacgccgg
    19981 acaggtggaa gaagcacggc tgcatcacgg tgttgatccg ccggtgcagc cccgcggcct
    20041 tcgccactgc ctcggcgtcg atcacgtacg tgcgcagctg cttgtcgatg atcagttgct
    20101 gcacctcgat cggcaggtgc tcccaggtct gctcgccgtg gggcgaattc agcaacagcg
    20161 tgccgccggg cttggcgacg tcgactgtct gcatcgcctt cagcagttcg aactggtgga
    20221 cggcaacgaa gtcggcctgg tcgatcaggt acgtcgaacg gatcggcgtg ctgccgaagc
    20281 gcaggtgcga aaccgtcacc gcgcccgact tgcgcgagtc gtagacgaag tagccctgcg
    20341 catgggcgtc cggacgcgat ccaatgatct tcaccgagtt cttcgccgcg ccgacggtgc
    20401 cgtccgaacc gagcccgaag aacactgccg agatgtgttc gctcgcgacg tggaagtcgg
    20461 tgtccggagc cagcgacagg tgggtgacgt cgtcgttgat gccgactgtg aaccgtggct
    20521 tggggttcgg cttcgccaat tcgtcgaaga ccgctttcgc cagttgtggg gtgaattcct
    20581 tggacgagag cccgtagcgg ccgccgatga cggttggcgc caccaggtca tcccggccca
    20641 ccaggtcatt ccggccctca acgtcattcc ggcgaaagcc gggatccccg tccgacgccc
    20701 gaggatcgtg cagggatccc gggccaagcc cgggatgacg agggccnnnn nnnnnnnnnn
    20761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20821 nnnnnnnnnn nnnnnnnnnn nnnnnngggc caagcccggg atgacgaggg cccgccgatg
    20881 ccagtgcgac cagcacatcg gcgtgcagcg gctcggcagg cgcgccgggt tccttggtcc
    20941 ggtccaggac ggcgatcgag cggacgctcc tcggcagcgc ggccaggaac gccgccaccg
    21001 ggaacggccg gtacaaccgg acggtgagca tgcccacctt ctcgccgcgg tgcaccaatt
    21061 cctcgacggt ctcggcaagg cagccggccg aagaacccat cgcaaccacg acgcgttcgg
    21121 cgtccggagc accgtggtag tcgaccagcg agtagcggcg tccggtgtgt tcggccagtt
    21181 cgccgaagac ttcggccacc acgtcgggca ctgcgaagtg gaacgggttg gcggcttcgg
    21241 cggcctggaa gaacgtgtcc gggttctccg ctacaccccg gatcatcggc gcgtccggcg
    21301 tcagcccacg cgagcggtgg gcgatcacgt ccgtctcgcg caccaacgcg cgcagcacgt
    21361 cgtcgcccag caactggatg gtgttctcct cgtggctggt gcggaagccg tcgaagaagt
    21421 gaacgaacgg cacccgcgtg cgcagcgtgg cggcgtgagc caccagcgcg aagtcgtgcg
    21481 cctcctgcac cgaggacgcg gacagcatcg cccagccggt catccgtgcg ctcatcacgt
    21541 cggagtggtc gccgaagatg ctcagcgcgt gcgtggcgac cgtccgcgcg gcaacgtgga
    21601 tcaccacagg cgtcagctcc ccggcgatct tgtacatgtt gggcagcatc agcagcagcc
    21661 cctgggacgc ggtgaaggtc gtacccaatg tccccttcgt gaccgcgccg tgcagcgccc
    21721 ccgcagcgcc ggcctccgac tgaagctcga ccacctcggg cacgccgccc caaagattcg
    21781 gccgcccggc cgccgaccag gcgtccgccg actcccccat cgccgaggac ggggtgatcg
    21841 gatagatcgc gatcacttca ctgagcgcgt gcgcgaccct ggccgcggcc tcgttcccgt
    21901 ccagagtggc gcggacgggc gcggcctcgc tggactcagc catcggtacc gcgctcctca
    21961 gtcaccgcac cattcttgcg cctcacaagg gctgacgacc caccgttcca acgatctggg
    22021 tgcggtactc cggactgctg aagcgggcgt actcggcctc ggccgcgcgg atccgggccg
    22081 gcacatccag tcgctcggac ggctcctcgt tgcccggcgt cgtgatgaac ttctgcagcg
    22141 cctgcagacc ggcggaagcg cgaccggcgg ctgcggtctg cttggcgtcg aggcgggcgg
    22201 cgtaccagtc cgaggccagc accgagtcgc ggctgaacag ggcgcggaac tcggggctgc
    22261 ccaggtccca gccttcggcg gacgtgccgt gcgccatgat ctccagcagc gccttgagcg
    22321 gcgggatcgc caactcgatc gtgccgtcgt cgaagtaggc ctgcgcgacc cgctgatgcg
    22381 tcgccacgat cgtcgccacc gattcggcga agatgtccgc gtcctgcagt tcgggacgca
    22441 gcatctcgtc ggtgaagacc gagtgcgggt gcaggaagat ccggccgaag tacttgcggc
    22501 tgaacgcctg ggtcatccgg tagcccagcc ggctggccag cacggtctca ccctcgtgct
    22561 cgaaatcctc gatgcgttcc agggcgccct cggcaagcag attggccgac gaccgctctg
    22621 cggcagtcat ccgcgagaac acctcaggca ccagcatcga gatgtcgtgg ttgacccgga
    22681 cgtacggccc gacgaacccg gcgcaggaca cccagccgtc gtagccggtg agcgcatagg
    22741 agaggagggc tgcgttcagg tcgacgatcg ccggcatcgc gttgaacggg cccttggtca
    22801 tcgccccctc ggagccggca ccggtggtgg acggcgactt gccggtcatc gaagagatga
    22861 attccgcgaa gagctccggt agttcaaggt agtgcagcgg actgaacgcg cacagcggcg
    22921 gcaccgggcc ctcgggcggg ttgttccggc gtccggcggc gacgatgtct accggcagcg
    22981 gcagcgcggc gtccgtgggc agcttgcgca ccaggtgcga agccaggtcg gcgaccgcgg
    23041 tgccgagtac gttggtgcgg tcgggacgct tctgcaggta gcgcgggttc ttgctcggct
    23101 tgccgtccac cagtcgtggg ctggccgagg agacgaagaa cttcggcgac acctccgccg
    23161 gcttgtcggc gaaatcgctg atcagattgc gcatcggagc ggtgaatttg ctgaaggcca
    23221 ccgcgtcgtc gcgcatctcg tcggcgtccg ccttcgtcag cggctggaag ttcgagagga
    23281 aagtgccggg cgaggcgatg tcgagttcgg cctgcttgtc gtagccgcga tggatggcgt
    23341 cgtccggacg ctggaacagc agctcctcgc agttctcgac cagcttgtag gagcgggtct
    23401 cgtccagacc gagcatgtgc ccgccgatca ccacggacgc ggtgatgtcg tcctcggtct
    23461 gcaccttcac ggctgggctg aaatcgtggc gcagcccgaa gagtcgccac gacccgtccg
    23521 catcgaagcc gacccggagc atgttcacca tgatcttgtc gccgtcgagg cgcaggttgt
    23581 tgccccggcg gccgttgatg atgccgacgc tgaagtggct gcgccagtcc tcgccccagg
    23641 ctggggtgta gaaccgcttc accacgaaga cgagttcctt gatgtgttgc ggaatctgct
    23701 ccagccaggc gttgtactcc ggcgagtact cgcgctcgga cggggtgagc agcttgatca
    23761 cgctaccgat ggaccgatcg tcggagagga tcggacgccg gtcggtgccg ttctgggtcg
    23821 gggcggcgaa gcgatcggag aagtcgtgct ccaggatggc ctgcacatgg tccatgtccg
    23881 cgtcgtagtc gggggtgtag gccgcgccga agatgaaagc gtcggtgatc gccttggaga
    23941 tttccgactt gccgccgccc gagaccgtgg acggcttgtg gcagctggtc gcggtgggaa
    24001 ccgtcccgac caggctccac tgggtgcgat cagcggcctg ctgggccatc cgcacctggt
    24061 agccgtccgg gctcaggtag accttgtcgg cgcgcagctt gagcgagccc gggttgccgt
    24121 ccgcgtccgt ccacgtgacc gtcatcgacc gcagcgagaa gttcgcgttc ggcggtacca
    24181 ggacgatctg ctcctgctgg cggtcgagcg cgtggccttc gggctgcaac tcgaagcgct
    24241 ccggattgcg ttcgaccacc tcggccaggt tgtactcgga atcgccgggc ggggtgacgg
    24301 tgtattcctg gcccaggttg tagctgggga agaccagggc gccgccggcg tgctcctcct
    24361 cggcgttgcc gaacaggttc gccgaatacg agatctgggt cttgacttcc ttcttgcagt
    24421 aaccgaagta gttgtcggcg atcaccgtga cgatcacccc acgggcatcc cgagcacaga
    24481 ccttgaacgc ctgcccagcg ttgtacaagt cgtcctcatg ctcccaggcc acaccgtcgc
    24541 ggcgctggcg ttcggtcgcc tcgctgatgt gcggcagccc gagcgacttc ttggtcacct
    24601 tggtcaggtg cggagccagg atcaccgcac cggtatgacc cgtccaggtc tccggcaaca
    24661 gggacgcatc attctccggc agatacggat cgccaccatt accgaagatg ccttcgacga
    24721 aatcgaggtt cgcgaccagg ccgcccggca cgatgaaccg ggtctccatc cgcttctccc
    24781 gcacataacc gggcaccgcc ggcgccacca acggacgcag ctgcagcgac acgaaacacg
    24841 ccgccgggtt ctccaggttc gccgtgaacg gcaggatctt gtcgacctcg ggcggctgga
    24901 aagccagttc cagcagccga gcgaacaccg ctttcgggac ggcgatcttg tcatccggaa
    24961 tcggcagccc accctcggcg atgtggaaca ccccggcggt cgtgcgccgg tcattggccg
    25021 ggttgtgcag caccccgttg gccagccggt agctgtgcag cagcggcgag ctgaactcgt
    25081 ccgcgtcata gggcagcgac aacgcccggg ccaaccccgg ctcatccagc acgaaggtgc
    25141 gcttgggcag cttcggctca accccagtct cagccaaata gtcgtccagc cagctctgaa
    25201 tccgcgcatc ggccgcacac aaccggtcgg caagccgacg actgagttcg cgctgtcggg
    25261 cgatgatggg cgccacgagc tttgcctggc cgtcctgcac ctcggctggc agcggcaacc
    25321 ccagctgggc aagtcgaagg ctaatggcgg cagtttcggt cgaagccatg ggttcattgt
    25381 cccaaacgat ttagcctcac caaaccccgt ccagcccgag ctatgcggtc ttgactgtgg
    25441 ctggatacac cgcggacggt gggctgaatc acagtcaacg cgtctcgcgt ggctgacgtt
    25501 gtccacaggc cggcattcat ccacatcccg gtagccgggc tgacgcggaa tcgtcgatcc
    25561 tgccgaagat gcagacgtga tcagtcgaag tgccgtcccg agcacgctca gccaaaccgc
    25621 cgaactccag gccggcatgc tctcgcgagc ccaggtgctg cgcgccggaa tcagtgatcg
    25681 cgtgatcgca cggctgtgtc ggcagcgctg gctgcaggtc gcgcccggcg tgtacagcgt
    25741 gcgcgacgaa ccgtcctggt tgggttgggc ctgggcgggc ctgctgatcg gcgggccagg
    25801 ttcggtactc ggcctggccg ccgctgggca cctgaaccag ctgatgccgg caccggatca
    25861 gctggccatc tgggtgggcg agcaccgcca ggtgcggacg cgttggccct ggcacttcat
    25921 tcgcggtcag cgcatcggag tcgggtcccc gccccggacg gaccgcatcc ggacgatcct
    25981 cgacctcgct gcggttcagc cgtccgatgc cctgctcaac ctgctgtctg aggcagtcgg
    26041 acgccggggc gtgacgccgt ccgtggtgct ggacgcgctg gcgcagttgc cgcgacaccc
    26101 ccggcggaag ctgttgattg aggtgctcgg tgacgtgact gccggcgtcc gctcgccatt
    26161 ggaacgccgt ttcctgcgcg acgtcgagcg cgcacatggg ctgcccgtcg ccgaacggca
    26221 ggaatcaccc agcggacggc atcaggtgga cgcgtggtac cgggagttcg gagtggttgc
    26281 ggaactggac ggtcgcgcgt tccacgaggg acggaaccgg ttccgcgact tcgagcggga
    26341 caaccggcat gcactgctga gcctgaccac gctgcggtat ggctggaacg acacgatcaa
    26401 caatccctgc tcggttgccc ggcaactcgt ggccatgttt cggctgcacg gctggacccg
    26461 cgtccccaag tcgtgcccgc gttgcgccaa ccggccttgg ccgtgacatc acccaccgcg
    26521 gacggtgcgc tgagtcacag tcaacgccgt ttcggcggag tagcctcacg ccatggaaac
    26581 gttggggccg gcgatcgccg agatcgcgat cgggttggcg ttctgcttct tcggctaccc
    26641 ggccgcacga ttcgtgctgg cgctgtgggg cgcggtgctc ggcttcatcc tcggcgtcgc
    26701 cggctacgcg gtcgtggtcg gctggctggg ctcgaacctg ggcaccatcg cgccgtggat
    26761 cgctggggcc atcggcgccg tcatccttgc cgggctcgcg ttcgcgttct actgggtgag
    26821 cgtgctggtg gcgatgggtt cgctgggctg ggggctcggg caggtgctgg ccgcgtcact
    26881 gaacgtgccc acttggctgg cggtcaccat cgcggtggtc gtcgcggccg gcctggtgat
    26941 catcggctgg acgctcgatc taccccggct cctgctgatc gtggtcacgg ccttcatcgg
    27001 ggccgccggg atcgtgaccg gcgtccaact gctgctcggc cagctgaacc cgtggtacgc
    27061 cgacctcggc caaagcctcg ccacgggcgc caacctggtc tggccggtcg ccttcgtgct
    27121 gctcggcatc atcggcgtcc tcgtccagtc ccggcagagc tcgggcaaga cgctccgcgc
    27181 cgcctaccac tgaccaccag cacgggcgat gagcacgggc aactcagcgc agcgtcccca
    27241 gcaccgcgga cgcgacctgc tcggcactgc g
//
LOCUS       DAOPRQ010000036        25520 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_1562_length_25520_cov_11.432869, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000036 DAOPRQ010000000
VERSION     DAOPRQ010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 25520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25520
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1562_length_25520_cov_11.432869"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1920
                     /locus_tag="PKJ61_06415"
     CDS             <1..1920
                     /locus_tag="PKJ61_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOC13493.1"
                     /translation="GMGGSMHAFFTPFGSMPNNAIVGGSAPIANGSALFKRVNRRPGI
                     VISNIGDASMGCGPVWEAMMFAAMDQFRQLWRDEDGGSPPILFNFFNNFYGMGGQTDG
                     ETMGYDILARVGAGVNPEAMHAERVDGYDPLAVADAIKRKKELLLAGKGPVLLDTITY
                     RFSGHSPSDASSYRTREEVEAWEQADSIKAFSDLLVAKKVLTTSGVEDLHGKLVERLT
                     KVLSLASNDDLERVDATYIEARMLSNEKVEAFGNGEEPELNEPLAENARVKAIAKKVR
                     TAKDENGKPVSKVKMYQFRDGLFEAMAHRYAIDPTMCAWGEENRDWGGAFAVYRGLTE
                     LLPYRRLFNSPISEAAIVGAGVGYALSGGRAVVELMYCDFLGRAGDEVFNQMAKWQSM
                     SAGLLKMPLVLRVSVGNKYGAQHSQDWSALVAHIPGLKVYFPANPTDAKGMLNLALAG
                     TDPVVFLESQLLYDIGEQFEVGGVPEGYYETAEGQPIVRREGTDVTIATLGATLYRAI
                     EAADVLQEKYGISAEVIDLRFVAPLDYAPVVESVKKTGRLVLASDAVARGSFLQTVAT
                     DIQNLAFDALDAPVVVVGARNHITPAPELEDLFFPQAEWIVDAIHERILPLPGHVPGA
                     NYTDGELIRRTRLGV"
     gene            complement(2020..2427)
                     /locus_tag="PKJ61_06420"
     CDS             complement(2020..2427)
                     /locus_tag="PKJ61_06420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13494.1"
                     /translation="MTKVLDQMVGRIAIAKSAARIAALTQLILDQVGASPDWQVASIT
                     SSGAGDGCVIEVALAVGGRPTGGTPVFTGIDVTDDRAVSAFVDQVYTVLIEESGGTLM
                     RFCPGHGHPSWPVSSEGNVLWVCLEAPRASLGR"
     gene            complement(2655..3635)
                     /gene="rlmB"
                     /locus_tag="PKJ61_06425"
     CDS             complement(2655..3635)
                     /gene="rlmB"
                     /locus_tag="PKJ61_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HOC13495.1"
                     /translation="MPGNSQRKGAVRKKGKTTPAGSGGRVRRGLEGKGPTPRAEDRPY
                     HKAYRKRRPDDPQKTVGGKPKGAKSGRSAGPSGTATAGADWVIGRNPVLEALQAGLPV
                     RRAYVADGVERDDRLRDILKFAAENGVPLLQATRAELDRLTGGGVHQGVALQLPAFEY
                     ADAEDVLGDALDAEAAGLVVALDSITDPRNLGAIVRSAAAFGAQGVVIPERRSAQMTA
                     AAWKTSAGAAARVPVARVTNLNRTLKTYADAGFTVVGLAGEAETDVAGFPGIDGPVLL
                     VVGSEGEGLARLVRENCDVLVNIPISSAVESLNASVAAAVALYEISRSRA"
     gene            complement(3643..5064)
                     /gene="cysS"
                     /locus_tag="PKJ61_06430"
     CDS             complement(3643..5064)
                     /gene="cysS"
                     /locus_tag="PKJ61_06430"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOC13496.1"
                     /translation="MSFRLYDSALRKVVEFEPLQPGKASIYCCGPTVQSAPHLGHIRK
                     EVNFDVLRRWLAASGYEVTMVTNVTDIDDKILIKSAEAGREWWAHAYHYEGVFHQAYR
                     ALGVLRPTYEPRATGHVPEMVELIATLIDRGHAYAAADGSGDVYFDVKSWPEYGWLSG
                     MKVDEMAPAEDADPRGKRDPRDFALWKGIKPGDPVTAAWDTPWGRGRPGWHLECSAMA
                     GRYLGDAFDIHGGGLDLRFPHHENEIAQSRAAGRPFAKYWMHNAWVTLAGEKMSKSLG
                     NTADVLAAVEAYGGRAVRLYLAGPHYRSAIELSDASLAEAAAQLARIDSFLDRASSLE
                     AGWTAAVPGEALPSAFVQAMNDDLGTPGAVAVLFQTVKEGNIALEAGDTAAAVRAHAA
                     VRAMLGVLGLDPAAPEWAESGASGDDLKPVVDGLVVALLDQRQQARARKDWPAADAIR
                     DQLAELGLKIEDTPNGPRWSIEK"
     gene            complement(5234..5587)
                     /locus_tag="PKJ61_06435"
     CDS             complement(5234..5587)
                     /locus_tag="PKJ61_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020574653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOC13497.1"
                     /translation="MITIDNSVAEPPYDQVKRQIAAMVASGELAPGDKLPTVRRLAAD
                     LGLAPNTVARAYRELEASGILDTRGRAGTFVTGDQTSRAARQAASEYAQRVRALGLNA
                     EAALALVQRSLAAEG"
     gene            complement(5584..6498)
                     /locus_tag="PKJ61_06440"
     CDS             complement(5584..6498)
                     /locus_tag="PKJ61_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13498.1"
                     /translation="MGAYAFSFEIPDFVLTAILVLPFIIAGLAYALPASAEHRLRRVH
                     SWATENVVTLTADVEVMLERVLTWRNRLSGVAMLLLGSLIGATALAWPDFWDSAGLQP
                     FSLVIVVLVAMTLALSFQFGQAWLPAGPTRTARPRAVGLADYVPSTLRTIAWISGATC
                     LAAIALMLGTQPTSWILAAQCTPQLSVVAALLFAEWNGRRAAAKPLPARNEIELYAQD
                     ALRTGLARAGFQSIALWGGLSLTMLPENLPWPAWLGVAFEFAGGALLLLSIVALLLPY
                     PAINWTRKRLWPRLRPGEYVGLDGLVLA"
     gene            complement(6695..8062)
                     /locus_tag="PKJ61_06445"
     CDS             complement(6695..8062)
                     /locus_tag="PKJ61_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002513114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4032 domain-containing protein"
                     /protein_id="HOC13499.1"
                     /translation="MPRFLSSRPDSRLIPLPWEIPLAEWPAENLVALPRGISRHVVRF
                     IKVGNSVYAAKEVIEHLAIHEYRMLADLHRTDTPAVEPLAVVTGRVGTQGQPLDPVLV
                     TRHLEFSLPYRSLFAQGVRPDTVGRLLDAMVVLLARLHLVGFLWGDVSLSNILFRRDA
                     GEFAAYLVDAETAEMHEQLTDGQREHDLTIATTNLYGEFCDLEAGEMLDASLDPQRLV
                     ESILTRYRELWAELTGVEEFSDQEVFRIEKRVRRLNALGFDVAELAIDTSPDGSSIRI
                     QPKVVDAGHHTRRLLRLTGLDVEEHQARRLLNDLDTFRARTNQGHVDEAVVAHRWLTE
                     CFQPVVTAIPSELRGKRDSAQIYHEVLDYRWYASQRENREVPLVEATWGYIQDVLRAL
                     PDEVMTDTRAIGEERQLANPYDPSQGFVDDEGPMPEDPWETAGDDLPAASGYLDIEAL
                     RARKA"
     gene            complement(8143..23280)
                     /locus_tag="PKJ61_06450"
     CDS             complement(8143..23280)
                     /locus_tag="PKJ61_06450"
                     /inference="COORDINATES: protein motif:HMM:NF039610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5979 domain-containing protein"
                     /protein_id="HOC13500.1"
                     /translation="MSLLLLALGLPITASAGEIDAEMTIEAFVTGHQGEVPTISPGEN
                     FSYTVNLQCSANDCQNAVVSAKLPAPLVYDDPAITVSPNLGTVAIDGQQLTITFVNDD
                     HALQLDAGQVVMVTVKATLPADASADYNGNTADITFDATADNADKVSDTASVKLNIEQ
                     KLKASATKSITPNSTQPSIPGRPATITLGGGSLANVSVDSIVLADPTDPTASGNPFSY
                     LALTGIDSLEAPGGADRVSFDYFNGTDWHNGAAVPIPDDPSTLIPSGVDPATITCVRF
                     TFTKDGGSLPSGSPAKIVLKTVSRGNIADGLDEGEVRTLTNTLTSTVTKGSETAKDEA
                     SATISFKKQSVQISASKSFDGPTLVVGKSTTVTLKATTGVMPTSTITINEPAADHENL
                     AEQGLIFGGFVTDPSADAQVTWPDGASKAEITYTYTDGTTATLSTTHADTLPAPTQPV
                     AGFSVTFTSADADGIESNALVRLPFTVTAGPVTEESGTVASNTISATASNAAGPPKSA
                     TVTKEITLLPKFVQTEVKKAFNRDWIWASAGATATAEVTGSVASESTIGSTRLVITDN
                     DQTFWDHFDLRRIVATDVPSNATMYPEYFDGTTWQPLTAPVVGPKTDWSYKLTDPQRA
                     AAQGVRFRFVGTDGSDLPAGFTVVPRFEVALRAQLRSAPGTSATVGVDELSNLVTSEV
                     ANDQAIQKLVTDTAKDTLALHPTGGGGGGNVDLVDKFWLDPEDKTYEENVSVSALSDD
                     IRTAAIRWGTSGLSMSTVEVVDDPHTGDVSASVFDAFDLVRIEPITAAIDPLMAKDKV
                     AKVELYLAGSGWTNITGAACAAGCDGTFGGYTLNDTERASATAVRLTFAPGTDSDTGA
                     VATNGNAHDRQVRLDLQLRRTLRSDSSTYVLGTSHSVKYNSGRAGVVLNNAEATGQLT
                     TPLDDGTKRITTLDSAQITIYDQPLNVSLTKQLDQTAVGLPQDTADSDAFPLVSATLT
                     ATNNTASGVPEFDIADPKPSSSTPWYDYLNLYQLQFAELPAGLSSDDVTVLLALADGS
                     DLVRSYAEAQALTAADLKDVVGVVVKYGAEANLKDPAKPLIPANGRAKLVLTYQLRGT
                     LRSTAAPVEPINHVANIAQATLVSPGGIGCADDQSQTCDKPSSTASGEIDIRQPDYAV
                     QAGKTISPGQRYEDEANGYTVTLTGQPCDADGSRCQRDSDKVFSTARTKLLTLTDDAP
                     TFWNAFDLDVLRPVTLPSPVNQLRLSVLTGVTYSLEGATLVAKCEGNTDLAACWRTGD
                     WSTAVNQQVTPTLPAGITADQVLGLRFEAQRVAADGTTVLQWERPADPKLSISFTTKQ
                     RTLLRYGTDGGSTTPVPSTLPGLAKAPGETTQGVTTDQLRVDGLAAWRNQGSPWIADD
                     QAVATTTLNHRVNQIRVEKTPGRGQATEAPRYDLDATIPYTFTVTNIGDWTMTGLELT
                     DTIGLVDDGKSKLVFADVDPKYTVKVNGVTQTGFTLTLDEDTGKLALGVPTEFRLAPD
                     GVLQIIANLRFRDRLTAGTEVANTLAVTSDRAFERCEYTKDAIEQDAITTGVAECAAS
                     TTVVAAASTPLTVTKAVKGSAAGDPAAASDSVNYDDLGVISVGAANAEECKRQREVGD
                     GYSTGNCAPITRPGGVETWRLSLTNNGNVAANVVSAIDVLPAPGDTGVTVNTARKSRF
                     TPTFLGNLKVTGLTSAAPHKLTYYYSTTAPSPSCNKNDILNDTKPDGQANCGTTWIAF
                     DGSTPAGDLAKAKAIKVLLKFDNKADGLRPGDPFYVSFDTRTPAYAPVADTNTVEPIA
                     WNSVAVGSRTAYSETFPWRASLITEPKKVGVAIASGQLDLAKAVVTPADVSWAGLLPS
                     SYPATVTCTSLDNTKLPTIPGSNPRQVSLPANGTPVSLNADRTMNLPLFADCTVVEDA
                     SQGADAAVTPAKVKALRDYSGIANVLTGWGAGTPAGKLTVTNTYREAGFTVSKAVDGP
                     AALQADGITPVAFKDFKFTASCTLLGHEVLADADKSFSLSPGGSRKFARLPAGADCSV
                     TETYAAGAASTKVVTTQGSTTVDSGTTKTTFSLVAGDETATKVDYTNTYTVGAATITK
                     SVTGPGAELWGNENFEVKLVCTHLDAITAPVFTASHTLTKATPTWTVSKLASGASCTV
                     TETKLGGANSNTITNSEFTVGTDPTKPTQIGVQNYFGLGQVSVTKKVLANGTNVSALA
                     PWSSGEFPVTLTCKRNVDGADKVITRTATLRAADDWTATWKDLPAAASCSVDENVGDI
                     TGVTGQPTPTKTITPATLNVPDGSTEAFTVTNNYRAGKLKITKELKGAGASFFTGATF
                     SVTCTMGGTTVYTNGSVSVPKGTLASAELGPIPFGSDCLVTETNSGGADADKTPGPQT
                     VSIVEPNSSNDVFTTNFTNYFSAGRVTVTKALAGAAKDEPWAKNAKFTFNVTCGKSAT
                     DPNRTTTTVTITGAGHTMVPNDTTPTLFPVGTSCWASETGVKGGATSTTVTPSAYDGT
                     KAVVPQADPSKPQELAVDATNTYEYAGLTVSKTVAGAVAQNQAGVTISYPTTVTFRAV
                     CTISNGASTTYPVLDQTFQLTGTGNGPWGTWTADKLAVGAGCRVTETQMPLTPDVSYR
                     ITRTGITPDLTGATLATSQFALVRGDTSVINGETAANKAAFTNTFRVGKLQLVKQLSP
                     SGTVWGTAPFTLRVSCTVDYPATGTVIYANDFPFTVTEANGIHTLDAISNLPYGAKCA
                     ISEPKTGGANASTIDVPNQTIGKDTTVTTKVTNTFNTGSVTVSKVLLVNGQSATAIEP
                     WASASYTMKLSCTRIVDGTPESLNMALVGGDTKVLKAPGFAATWTGLPQDATCRATET
                     TIEYPPLTPTQPDPTVTYSGDAVVGNGTTASFTVTNDFKFGNVQIVKSLAGEAAAKWG
                     TGTFSFKVECTLAGTAGYVFTRTGITLTAPNLTSAAIGPIPEGADCTVTETNSAGATS
                     VAPGTTVVLKNLPSGTTTAANFTNTFLYAGFTVTKTVDNGGALDASGDQVIYRRSFDY
                     RAVCTFNGNQILNQTFTLHHVVAKTWETKAFEQLPSGASCTVTETGTGEAASTGVKVT
                     QNGSTLVDSPSSPTATFNLATGTSATTTAAFTNHFTTGGLDLSKVVTGAGAGLWGAGP
                     FTVRVVCTFDDDLVVGTPAATAYDGTKDLTGGDTWKLRNLPTGANCTVSEPKTGGANA
                     TTVVPSTLTIGTTNQPVTITNTFTVGSVQVTKAIADGLGLLAPWKDTVYEFTLSCTKD
                     FNGDGTAESFPVPNPVKTVTGPGSVIWAGLPTGASCAAAETGITYPAGTPTQPAPVVT
                     STGGVTIAAGETKGQTLTNTFSTAKVQITKTITGDAATTWGTGPFTFTVACTLGGSGS
                     VFSKDVTLTPSAGQTSLASAELGPIPPGSNCLVTETSSAGATSVEPASRQVALTQLEA
                     NKTTPAAFTNEFRFAGFTVTKSVVSAALDANGKPIAYDPASFTASCLFKGAEVLTNPS
                     DRAFTLADKQSHSFAGLPSGAACTVTETGTAGAASTEVTITQDAASSTTTATTAKFNL
                     VPGTSTATKVGFANHYTVGSLAITKTVDGTGAARWGGGNFGVRVICSLSQANPDQVFD
                     GVHTLSKADPTWQIDNLPTGADCQVTETTDGGANSTVITNPSPTIGKDTVVETGITNT
                     FTLGAITVTKALTVDGETTNDAPWTQGSYTVELACTRDFDNNGTAEALAIPGGAVRVI
                     TGAETATYANLPTGASCSVTEKASSPLAQATSITPSGALTVGADQTTPGAFTVTNDFH
                     TGPLSVLKQLAGAGQESFGNGPFTFTVSCTLAGATDPVYTTTFDLARPAGSTVTSLRK
                     DDLGPIPVGAECLVKETVRGGADAPPAPVTLTIDADAANNVATFTNRFSAGTITLAKT
                     LDGAAKAEPWATAATFKVRVTCEVEVDGERGQVFSSDVAIKGGQTIAVPVADDNPSRV
                     PLGSHCWAAETDAQGAGKVTIEHHDWDSALVVVGNPDKLQTLDLGVLNTFEYAGFTVT
                     KTVDNGGAVDADNNPIAYPASFGYHAQCTFNGVAVLNETFTLLDGGSKPFAGLPAGAD
                     CQVNETATGNAASTSIQVTQNGAQLDTGGLARFVLVRGTSGDPAASSVTTAAFTNHFT
                     TGALTITKKLTGAGADAWGKATFTVGLVCTFDTDADPATPDAEVYRASKDLSIGTTLV
                     WPVTKLPTGASCAVSEIAHGGANSTTITNPIPLIGNDPAEPVAVDVTNEFTLGSVTVT
                     KKVLVDGTADSEAEPYHTASFQVRLKCSRLVNDEIEGIDIPGDAYPLGDDRDGVRTII
                     GSDSHSYDGLPTGATCLVSEVGASLPLPSDQISIEQPEPVAAAPTVVNGVVSNDYHTG
                     SLQLHKQLTGDGVADWADADFTFDVSCTLTDTAEVAHVVFTRTGIVLNRENPDSDVYT
                     GIPVGANCVVHETADGGADVPSADVTVTIADGPTTVATPTNQFNLGALKVTLGLTLDG
                     EPTTADPFAGGSYAIEVTCTRVVNGETVAVPVPGGGSWTFAVASDREHLFTGLPVGAS
                     CSVHQSTASLTPQDVSYTPANAEPSGVGSGKVTIGSDPQSPAALGVIDHFRTSTLTVT
                     KRLTGSGLEEHASYPFTISVACTLAEDGVDVPHPIFDTTITLSVIGGLVSEPLGRIPV
                     GSHCVVTETGTGGATIPAEPAELTISEDGPNAVTLTNAFNTGSVIITKAITVDGVASA
                     AEPYASATYTVGLACTQLVDGVPMPVVIPGGATRIIKGAGSAKYDEVPLGADCTVSET
                     GSTLAIPSDQVTISKPKVTVGAEPVESVITNDFRTGTLVVTWQLSGVGTDFAGPATFA
                     VECTLDGATGWAYRNEFTLDPSGAQTLAATGGLTVVGPTLAPIPVGAHCTVTQSGAQG
                     ADAVASAVVASGTVSLTATLANEYSAGTLTIVKQLAGKGAKHEVDTEFAFEVTCQLPD
                     RTPYFHDIVRVVGAGSATVSAADGRPRLFPAGVHCWAIETVTGGADSHQVDRPSFEQA
                     ATVVTGQPQALQPMQVTAVNNFKDPNANDSASDSDGDGALAFTGFAGWALGGFGLTLV
                     LAGAVLVLRRRRV"
     gene            complement(23534..24631)
                     /gene="ugpC"
                     /locus_tag="PKJ61_06455"
     CDS             complement(23534..24631)
                     /gene="ugpC"
                     /locus_tag="PKJ61_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012918724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HOC13501.1"
                     /translation="MATVSFKDATRVYPGSDHPAVDKLNLEIGDGEFMVLVGPSGCGK
                     STSLRMLAGLEEVNSGQILIGDRDVTDLPPKDRDIAMVFQNYALYPHMTVADNMGFAL
                     KMAGVPKAERDQRVLEAAHLLGLESFLGRKPKALSGGQRQRVAMGRAIVRQPQVFLMD
                     EPLSNLDAKLRVSTRTQIAALQTRLGVTTVYVTHDQVEAMTMGDRVAVMKDGILQQVD
                     SPLTLYDRPANLFVAGFIGSPAMNLISGKIVEGGVQIGDYVVPVAREVLAKAAGEESL
                     VLGIRPENFRIAETGIGLEVDVVEETGADAYVYGTLAGLDAEQKLTAQQIVARVSART
                     PPQRGTVVKLNTVEAETMHVFSEKTQERLSA"
     gene            24803..25519
                     /locus_tag="PKJ61_06460"
     CDS             24803..25519
                     /locus_tag="PKJ61_06460"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HOC13502.1"
                     /translation="MSDVQPETAGDQPAAEREWWDDPSLPWRHKPTRADIACFSWLGA
                     VAIYSIVISVLRPGLLASAPHVLASLGSWSGTILIGARAAVGDPWWLLVWVLGTLGMI
                     KFDWIYWWAGKLWGRSLIEAWSGRSERARRANAWAERFARKYEVLAIAITFLPIPLPR
                     GVIFAVLGEAGTSLRKFLTVAAISSFISTGGYLALGYWIGEPAVAVMDAYGRYLWIVS
                     LVILVVVVYNAWRKGAPKPV"
BASE COUNT         3641 a         8157 c         9269 g         4453 t
ORIGIN      
        1 ggcatgggcg gctcgatgca cgccttcttc accccgttcg gctcgatgcc caacaacgcc
       61 atcgtcggcg gctcggcccc gatcgccaac ggctcggcgc tgttcaagcg ggtcaaccgc
      121 cgccccggca tcgtgatctc caacatcggc gacgcctcga tgggctgtgg accggtgtgg
      181 gaagcgatga tgtttgccgc gatggaccag ttccgtcagc tgtggcgcga cgaggacggc
      241 ggcagcccgc cgatcctgtt caacttcttc aacaacttct acggcatggg cggccagacc
      301 gacggcgaga ccatgggcta cgacatcctg gcccgggtcg gcgcgggtgt gaaccccgaa
      361 gccatgcacg ccgagcgggt cgatggttac gacccgttgg cggtcgcgga cgcgatcaag
      421 cgcaagaagg agttgctgct ggccggcaag ggcccggtgc tgctggacac catcacctac
      481 cggttctccg gccacagccc gtccgatgcg tcgagctacc ggacccgcga agaggtcgag
      541 gcctgggagc aggccgactc gatcaaggcc ttctccgacc tgctggtcgc caagaaggtg
      601 ctgaccacct ccggcgtcga agacctgcac ggcaagctgg tcgagcggct gaccaaggtg
      661 ctctcgctgg cctccaacga cgacctcgag cgggtggacg ccacctacat cgaggcccgg
      721 atgctcagca acgagaaggt cgaggcattc ggcaacggcg aggagccgga gctgaacgag
      781 ccgctggccg agaacgcccg ggtgaaggcg atcgccaaga aggtccgcac cgccaaggac
      841 gagaacggca agccggtctc gaaggtgaag atgtaccagt tccgcgacgg cctgttcgag
      901 gcgatggcgc accggtacgc gatcgacccg accatgtgcg cctggggcga ggagaaccgt
      961 gactggggcg gcgcgttcgc cgtttaccgc ggcctgaccg agctgctgcc gtaccggcgg
     1021 ctgttcaact ccccgatctc cgaggccgcg atcgtcggcg ccggcgtcgg ctacgcgctg
     1081 tccggcggac gggccgtggt cgagctgatg tactgcgact tcctcggccg cgccggtgac
     1141 gaggtgttca accagatggc caagtggcag tcgatgtcgg ccggtctgct gaagatgccg
     1201 ctggtgctgc gggtgagcgt gggcaacaag tacggcgccc agcactccca ggactggtcg
     1261 gcgctggtcg cgcacatccc cggcctgaag gtgtacttcc cggccaaccc gaccgacgcc
     1321 aagggcatgc tgaacctggc cctggccggc accgacccgg tggtcttcct cgaatcccag
     1381 ctgctctacg acatcggcga gcagttcgag gtcggcggtg ttcccgaggg ctactacgag
     1441 accgccgagg gccagccgat cgtccgccgc gaaggtaccg acgtgaccat cgccaccctg
     1501 ggcgcgaccc tctaccgggc gatcgaggcc gccgacgtgc tgcaggagaa gtacggcatc
     1561 tcggccgagg tgatcgacct gcgcttcgtc gcgccgctcg actacgcccc ggtcgtcgag
     1621 agcgtgaaga agaccggacg cctggtgctg gcctcggacg cggtcgctcg cggctcgttc
     1681 ctgcagacag tggccaccga catccagaac ctggccttcg acgcactgga cgccccggtg
     1741 gtcgtggtcg gcgcgcgcaa ccacatcacc ccggcccccg aactggaaga cctgttcttc
     1801 ccgcaggccg aatggatcgt cgacgccatc cacgagcgca tcctgccgct gcccggacac
     1861 gtcccgggtg ccaactacac cgacggtgaa ctgatccgca ggacccgcct gggcgtctga
     1921 ccttcccgag cctgcggggc ccttacgttc gagggtcgag cagccgaaat cgtcgagggc
     1981 cagtcttcgg cgctgagaac cagccacagc ggctggcgct cagcgtccaa ggctggccct
     2041 cggtgcttct aggcagaccc acagcacgtt gccttcggac gacaccggcc agcttgggtg
     2101 cccatgaccg gggcagaaac gcatcaaagt accgcccgac tcctcaatga gcacggtgta
     2161 gacctggtca acgaaggccg acacagcgcg gtcgtcggtg acgtcgatgc ccgtgaacac
     2221 cggcgtgcca cccgttggcc ggccaccgac cgccagggct acctcaatca cgcagccgtc
     2281 cccggctccg ctgctggtga tgctcgcaac ctgccagtct ggtgatgcgc ccacttggtc
     2341 aagaatgagc tgagtaagcg cagcgattct ggccgccgat ttagcgatcg cgatccttcc
     2401 caccatctgg tcgagaacct tcgtcaagct caagaatcgt tccgacgctt cctgaggtct
     2461 tcccggaaac ccaatcttgc tgggagatcc cggcctgcgc cgggatgacg gtggaaccgg
     2521 gctctgggga cgttacgcgc gggagcggct gatctcgtcg taccgcaccc gcttgcgggt
     2581 gctcagcaag agatcaggcg cgggagcggc tgatctcgtc gtaccgcacc cgcttgcggg
     2641 tgctcagcaa gagatcaggc gcgggagcgg ctgatctcgt ataacgccac cgctgcagcc
     2701 acggacgcgt tcagcgactc caccgctgag ctgatcggga tgttcaccag cacgtcgcag
     2761 ttctctcgca ccaggcgcgc caagccctcg ccttcggagc cgacgaccag cagcaccggc
     2821 ccgtcgatcc ccgggaagcc cgccacgtcg gtctcggcct cgccggccag gccgaccacg
     2881 gtgaagccgg cgtccgcgta ggtcttcagg gtccggttca ggttcgtcac ccgggccacc
     2941 gggacgcggg ccgcggcgcc ggcggacgtc ttccacgccg cggcggtcat ctgcgccgag
     3001 cgtcgctccg gaatcaccac accctgcgcg ccgaaagcgg cggcgctccg cacgatcgcg
     3061 ccgaggttgc gcgggtcggt gatcgaatcc agcgccacca cgagcccggc ggcctcggcg
     3121 tccagcgcat cgcccagcac gtcctctgcg tccgcatatt cgaaggccgg caactgcagc
     3181 gccacgccct gatggacgcc gccgccggtc agccggtcga gttcggcgcg cgtggcctgc
     3241 agcagcggaa cgccgttctc agcagcgaac ttcaggatgt cgcgcagccg gtcgtcgcgt
     3301 tcgaccccgt ccgctacata ggcgcgccgc acgggcagcc cggcctgcag cgcctccagc
     3361 accggattac ggccgatcac ccaatccgcg ccggcggtcg cagtgccgga cggccccgcc
     3421 gaccgacccg acttggcacc cttgggcttg ccgccgacgg tcttctgcgg atcgtccgga
     3481 cggcgcttgc gataggcctt gtggtaaggc cgatcctcgg cgcgcggggt cggccccttg
     3541 ccttcgaggc cacgccggac gcgtccgccc gacccggccg gggtggtctt gcccttcttg
     3601 cggaccgcgc ccttgcgctg gctgttccct ggcatgtcgc tcctacttct cgatcgacca
     3661 gcgcgggccg ttgggtgtgt cttcgatctt caggccgagt tcggccaact ggtcacgaat
     3721 ggcatcggcg gccggccagt ccttgcgggc gcgggcctgc tgccgctggt cgagcagcgc
     3781 cacgaccagg ccgtcgacca ccggcttcag gtcatccccg gacgcacccg attcggccca
     3841 ttccggggcc gcggggtcaa gcccgagcac accgagcatc gcgcggacgg ccgcgtgcgc
     3901 gcggacggcg gcagccgtgt cgccggcttc gagggcgatg ttgccctcct tgaccgtttg
     3961 gaacagcacc gcgaccgcgc ccggggtgcc gaggtcgtcg ttcatcgcct ggacgaaggc
     4021 ggatgggaga gcctcgccgg gcaccgcagc cgtccagccc gcttcaaggg aggatgcgcg
     4081 atcaaggaac gagtcgatcc gggccaactg ggcagcggcc tcggccaggg acgcgtccga
     4141 cagctcgatg gctgagcggt agtgcgggcc ggccaggtag agccgcaccg cgcgtccgcc
     4201 gtaggcctcg accgcggcca gcacgtcggc ggtgttgccc agcgacttgc tcatcttctc
     4261 gccggccagg gtcacccagg cattgtgcat ccagtacttg gcgaacggac gcccggccgc
     4321 gcgcgactgg gcgatctcgt tctcgtggtg cgggaagcgc aggtcgagcc cgccgccgtg
     4381 gatgtcgaag gcgtcgccga ggtatcggcc ggccatcgcg gagcattcca ggtgccagcc
     4441 cggccggccg cgtccccacg gcgtgtccca ggcggcggtt accggatcac cgggcttgat
     4501 gcccttccac agcgcgaagt cgcggggatc ccgcttgccg cgggggtcgg catcctcggc
     4561 cggcgccatc tcgtccacct tcatgcccga gagccagccg tattctggcc aggactttac
     4621 gtcgaagtag acgtcgccgg aaccgtccgc ggcagcgtag gcgtggcccc gatcgatcag
     4681 ggtggcgatc agctcgacca tctccggcac atgcccggtg gcgcgtggtt cgtaggtcgg
     4741 acgcagcacg ccgagcgcac ggtaggcctg atgaaaaacg ccctcgtagt ggtacgcgtg
     4801 cgcccaccac tcgcggccgg cttcggcgga cttgatcagg atcttgtcgt cgatgtcggt
     4861 cacgttggtg accatcgtca cctcgtaccc ggacgccgcc agccagcgcc gcaggacgtc
     4921 gaagttgacc tccttgcgga tgtggcccag gtgcggagcc gactgcacgg tcgggccgca
     4981 gcagtagatc gaggccttgc ccggttgcag gggctcgaac tcgaccactt tgcgcagcgc
     5041 gctgtcgtaa aggcggaaac tcaccgccgt atcttatcgg ttgccgccgc ggctctcacc
     5101 ggtggcctga gagctagcag actgcgtcga gcgctagcac cagaggctgg cggtcgaacg
     5161 ctacggctgg cgctcgccgt ggaagggggc tggcactcgc cgaaggaggg gccgcatgga
     5221 tgggcggtga gccttagcct tcggcggcga ggctccgttg caccagggcc agggccgcct
     5281 cggcattcag cccgagcgct cggactcgct gggcgtactc ggacgccgcc tgccgcgccg
     5341 cgcggctggt ctggtcgccg gtgacgaagg tgccggcgcg tccgcgggtg tcgaggatgc
     5401 cggacgcctc cagctcccgg tacgcccgag ccaccgtgtt cggggccagg ccgaggtcgg
     5461 cggccagccg gcggacggtc ggcagcttgt ctccgggagc cagttcaccg ctggccacca
     5521 tcgccgcgat ctgacgtttc acctggtcat agggcggttc ggcgactgag ttgtcgatag
     5581 tgatcatgcc agcaccaatc cgtcgagccc gacatactct cccggacgca gccgcggcca
     5641 gagccgcttc ctggtccagt tgatcgcagg gtagggcagc agcaacgcga cgatcgagag
     5701 cagcagcagc gcgccaccgg cgaactcgaa tgccaccccg agccaggcgg gccaaggcag
     5761 gttctcgggc agcatggtca ggcttaagcc accccagagg gcgatgctct ggaatccagc
     5821 gcgggccagc ccggtgcgta gggcgtcctg ggcgtacaac tcgatctcgt tgcgcgcggg
     5881 cagcggtttc gcggcggccc tgcggccgtt ccactccgca aacagcaggg ccgcgaccac
     5941 gctcagttgg ggcgtgcatt gggcggccag gatccacgaa gtcggttggg tgccgagcat
     6001 tagcgctatc gctgccaggc acgtcgcgcc gctgatccag gcgatcgttc tcaaagtgct
     6061 gggcacgtaa tcagccagcc ccacggcgcg ggggcgagcc gttcgagtcg gtccggctgg
     6121 cagccaggcc tgaccgaact gaaaactcag cgcgagggtc atcgccacca agacaacgat
     6181 caccagactg aaaggctgca acccggccga gtcccagaag tcgggccagg caagcgcggt
     6241 ggcgccgatg agtgatccca gtagcagcat cgccaccccc gagagccggt tgcgccaggt
     6301 cagcacacgc tcgagcatca cctctacgtc tgcggtcagc gtgaccacgt tctcggtcgc
     6361 ccagctgtgc acgcgtcgca atcgatgctc tgcggacgcc ggcaacgcgt acgccaaccc
     6421 agcgatgatg aacggcagca cgaggatcgc ggtaaggacg aagtctggaa tctcgaagct
     6481 gaacgcgtac gcacccatgc cctcatgcta cataacgggt gattgtgtag caatgcattg
     6541 gttgatacat aggtgacgga cggcccttcg tgacgcccgc tcgtgcctcg cgggctcctc
     6601 agggatcgtt ggtggggccg ctcgtgcctc gccggctcct cagggatcat cgatttcggc
     6661 tggacgagct tgtcgagacc cgctcgccgg tggctcaagc tttgcgggcg cgtagagcct
     6721 cgatgtcgag gtagccggac gcggccggca ggtcgtcgcc ggcggtctcc caagggtcct
     6781 cgggcatcgg gccctcatcg tccacgaagc cctgggacgg gtcgtagggg ttggccagct
     6841 gacgttcctc gccgatagcg cgggtgtcgg tcatcacctc gtcgggcagg gcgcgcagca
     6901 catcctggat gtagccccag gtggcctcca ccaggggcac ctcgcggttc tcccgctgcg
     6961 atgcgtacca gcggtagtcg agcacctcgt ggtagatctg ggcgctgtcc cgcttgccgc
     7021 gcagctcgga cgggatcgcg gtcaccaccg gctggaagca ttcggtgagc caccggtgcg
     7081 cgacgaccgc ctcgtccacg tggccctggt tggtgcgggc ccggaaagtg tccagatcgt
     7141 tgagcagccg ccgagcctgg tgctcctcga cgtcgagtcc ggtcaggcgc agcagccggc
     7201 gggtgtggtg gccggcgtcc acgaccttgg gctggatccg gatcgaggag ccgtccggcg
     7261 aggtgtcgat ggccagttcg gcgacgtcga agccgagggc gttgagccgt cgcacccgct
     7321 tctcgatccg gaagacctcc tggtcggaga actcctcgac cccggtcagc tctgcccaca
     7381 gctcccggta ccgggtgagg atcgactcga ccagccgctg cgggtcgaga gatgcgtcca
     7441 gcatctcgcc cgcctccagg tcgcagaatt cgccgtagag gttcgtggtg gcgatggtca
     7501 ggtcgtgttc gcgctggccg tcggtgagtt gctcgtgcat ctcggcggtc tcggcgtcca
     7561 ccaggtaggc ggcgaactcg ccggcgtccc ggcggaacag gatgttcgac aacgacacgt
     7621 ccccccacag gaatcccacg aggtgcaggc gggccaggag gaccaccatc gcgtccagga
     7681 gccgtccgac ggtgtccgga cggacgccct gggcgaacag tgagcggtag gggagcgaga
     7741 actccagatg ccgggtgacc aggacgggat ccagcggctg gccctgcgtc ccgacccgcc
     7801 cggtgaccac agccagcggc tccaccgcag gggtgtcggt gcggtgcagg tcggccagca
     7861 tccggtactc gtggatcgcc aggtgttcga tgacctcctt ggcggcgtac accgaattgc
     7921 ccaccttgat gaagcggacg acatgtcggg agatgccgcg ggggagcgcg accaggttct
     7981 cggcgggcca ttccgctagc gggatctccc agggcagcgg gatcagccgg gaatccgggc
     8041 gggaagagag gaaccggggc acgcctcggt tatacaccac cggacgcagc cgcccggcgt
     8101 ggcggggaga ccggaacctg cccggcctcc cctgagcccc ggtcagaccc ggcgacgcct
     8161 gagcaccagc accgcgcccg cgagcaccag cgtcagcccg aaaccgccga gcgcccagcc
     8221 ggcgaacccg gtgaacgcca gggcaccgtc accgtcggag tcactggcac tgtcgttggc
     8281 gttcggatcc ttgaagttgt tcacggcggt cacctgcatc ggctgcagcg cctgcggctg
     8341 cccggtgacc accgtggccg cctgctcgaa gctcggccga tccacctggt gggagtctgc
     8401 cccgccggtc accgtctcga tcgcccagca gtgcactccg gccgggaaca accgcggccg
     8461 gccgtccgcg gccgaaacgg tcgccgagcc ggcgccaacc acccgcacga tgtcgtggaa
     8521 gtacggcgtc cggtcgggca gctggcaggt gacctcgaag gcgaactcgg tgtcgacctc
     8581 gtgcttggcg cccttaccgg cgagctgctt gacgatggtc aaggtgccgg ccgagtactc
     8641 gttggccagc gtcgcggtca ggctcaccgt cccgctcgcc accaccgcgg acgcgaccgc
     8701 gtccgctccc tgggcacccg actgggtcac cgtgcagtgc gcgccgaccg ggatcggggc
     8761 cagggtcggc ccgactacgg tcagtccgcc ggtggcggcc agggtctgcg ccccggacgg
     8821 atccagggtg aactcgttgc ggtaggccca gccggtggcc ccgtccaggg tgcactcgac
     8881 cgcaaaggtc gcggggccgg cgaagtcggt gcccaccccg gacagctgcc aggtgaccac
     8941 gagcgtcccg gtacggaagt cgttggtgat caccgactcg accggttcgg cgccgacggt
     9001 gaccttcggc ttgctgatcg tcacctgatc ggacgggatc gccagcgtgc tgccggtctc
     9061 cgagacggtg cagtccgcgc cgagcggtac ttcatcgtac ttcgcgctgc cggcgccctt
     9121 gatgatgcgg gtggccccac cggggatcac caccggcatc gggacgccgt ccaccagctg
     9181 ggtgcaggcc aggccgacgg tgtaggtcgc gctggcgtag ggttcggcgg cgctggccac
     9241 gccgtcgacg gtgatcgcct tggtgatgat caccgagccg gtgttgaacg cattggtcag
     9301 ggtgaccgcg ttcggtccgt cctcgctgat ggtgagctcg gccggctcgg ccggaatcgt
     9361 cgctccgccg gtgccggtct cggtgaccac gcagtgcgag ccgaccggaa tccgtccgag
     9421 cggttcgctg accagcccac cgatgacgct cagggtgatc gtggtgtcga agatcgggtg
     9481 cggcacgtcg acgccgtcct cggccagcgt gcaggccacg ctgatcgtga acggatagct
     9541 ggcatgctcc tcaaggccac tgccggtgag ccgcttggtc accgtcaggg tgctggtgcg
     9601 gaagtggtcg attacgccca gggcggctgg cgactgcggg tcggaaccga tcgtcacctt
     9661 gcccgaaccc acgccggacg gctcggcgtt ggccggggtg tagctgacgt cctgaggagt
     9721 caacgacgcc gttgactggt gcaccgagca ggacgccccc accggcagcc cggtgaaaag
     9781 gtgctcccga tcggacgcga ccgcgaaggt ccagctgcca ccgccgggca ccgggacggc
     9841 gacggtctcg ccgttgacca cccgggtgca ggtgacctcg atcgcgtagc tgccgccggc
     9901 gaacggatcg gccgtggtcg gctcgccgtc cagggtcagc ccgagggtga ccttcagcgc
     9961 gcccaggttg aactggttgg tgggcgtggc caccgtggtc ggaccgtccg cgatggtgac
    10021 cgtgacgtcg gcggacggga cgtccgcgcc gccgtccgcg gtctcgtgga cgacacagtt
    10081 cgcgcccacc ggaatgccgg tgtagacgtc gctgtccggg ttctcccggt tcagcacgat
    10141 gccggtcctg gtgaacacga cgtgcgccac ctcggcggtg tcggtcaggg tgcagctgac
    10201 gtcgaaggtg aagtcggcgt cggcccaatc ggcgaccccg tcgccggtca gctgcttgtg
    10261 cagctgcaac gaacctgtgt ggtaatcgtt gctgacaacg ccgttcacca ccgtcggggc
    10321 ggccgcgacc ggctccggct gctcgatgct gatctggtcg ctgggcagcg gcaggctggc
    10381 gcccacctcg ctgaccagac aggtcgcacc ggtcggcagg ccgtcgtagc tgtggctgtc
    10441 cgaaccgatg atggtgcgga cgccgtcgcg gtcatcaccg agcgggtagg cgtcgccggg
    10501 aatgtcgatg ccctcgatct catcgttgac cagccgggag cacttcagcc gcacctggaa
    10561 ggacgcggtg tggtacggct cggcttcgga gtcggccgtg ccgtcgacca gcaccttctt
    10621 ggtcaccgtc accgaaccga gggtgaactc gttggtcaca tcgaccgcga ccggctcggc
    10681 cggatcgttg ccgatcagcg ggatcgggtt ggtgatggtg gtgctgttgg cgccgccgtg
    10741 ggcgatctcg ctgaccgcac aggacgcgcc ggtgggcagc ttggtcaccg gccagaccag
    10801 ggtggtgccg atcgagaggt ccttgctggc ccggtagacc tcggcgtccg gggtggccgg
    10861 atcggcgtcg gtgtcgaatg tgcagaccag gccgacggtg aaggtggcct tgccccaggc
    10921 gtccgctccc gcgccggtga gcttcttggt gatggtgagc gcaccggtgg tgaagtggtt
    10981 ggtgaaggca gcggtggtca ccgaggaggc ggccggatcg ccgctggtgc cgcgcaccag
    11041 gacgaacctg gccaggccgc cggtgtccag ctgggccccg ttctgggtga cctggatcga
    11101 ggtgctggcg gcgttgccgg tcgcggtctc gttcacctgg cagtccgcgc ccgccggcag
    11161 accggcgaag ggcttgctgc cgccgtcgag cagggtgaag gtctcgttca gcaccgcgac
    11221 cccgttgaag gtgcactggg cgtggtagcc gaagctggcg gggtaggcga tcgggttgtt
    11281 gtcggcgtcg accgcgccgc cgttgtcgac ggtcttggtg acggtgaagc cggcgtactc
    11341 gaaggtgttc agcacaccca ggtcgagcgt ctgcaacttg tcggggttcc cgacgaccac
    11401 cagcgcgctg tcccagtcgt ggtgttcgat ggtcaccttg ccggcgccct gagcgtcggt
    11461 ctcggcggcc cagcagtggc tgcccagcgg cacccgggac gggttgtcgt cggcaaccgg
    11521 gacggcgatg gtctgcccgc ccttgatcgc cacgtcgctg gagaacacct gaccgcgctc
    11581 tccgtccacc tcgacctcac aggtgacccg caccttgaag gtggccgcgg tggcccacgg
    11641 ttcggccttg gccgcgccgt ccagggtctt ggccagcgtg atcgtgccgg cgctgaaccg
    11701 gttggtgaag gtggccacgt tgttggccgc gtccgcgtcg atggtcaggg ttaccggggc
    11761 cgggggagcg tccgctccgc cgcggacggt ctccttgacc aggcactccg caccgaccgg
    11821 gatcgggccc aggtcgtcct tgcgcaggct ggtgaccgtg ctgccggccg gacgggccag
    11881 gtcgaaggtg gtggtgtaga ccgggtcggt cgccccggcc agcgtgcagc tgaccgtgaa
    11941 ggtgaacggc ccgttgccga aggactcctg accggcaccc gccaactgct tgagcaccga
    12001 cagcggcccg gtgtggaagt cgttggtgac ggtgaaggca cccggcgtgg tctggtcggc
    12061 gccgacggtg agcgcaccgg acggggtgat gctggtcgcc tgggccagcg gggagctggc
    12121 cttttcggta accgagcagg acgcgccggt gggcaggttg gcgtaggtcg ccgtttcggc
    12181 tccggtgatc acccgcacgg cgccaccggg aatggcgagc gcctcggcgg tgccgttgtt
    12241 gtcgaagtcg cgggtgcagg ccagttccac ggtgtagctg ccctgggtcc acggcgcgtc
    12301 gttggtggtc tcgccgtcca cggtcagcgc cttggtgacc gtgatcgccc ccagggtgaa
    12361 ggtgttggtg atcccggtct cgacgacggt gtccttgccg atggtgggcg aggggttggt
    12421 gatgacggtg ctgttcgcgc cgccatcagt ggtctcagtc acctggcagt ccgcgccggt
    12481 cggcaggttg tcgatctgcc aggtcgggtc ggccttggac agggtgtgga cgccgtcgaa
    12541 gacctggtcg gggttggcct gactcaggct gcagatcacg cggacgccga agttgccgcc
    12601 gccccaccgc gccgcgcccg ttccgtccac ggtcttggtg atcgcgagcg atccgacggt
    12661 gtagtggttg gcgaaaccaa ctttggtggc cgtgctggtg cccggcacca ggttgaactt
    12721 ggccgtggtc gcggtcgtgg tgctgctggc ggcgtcctgg gtgatggtca cctcggtgga
    12781 cgccgctccg gccgtcccgg tctcggtgac cgtgcaggca gcgccactgg gcaggccagc
    12841 gaagctatgg ctctgcttgt cagcgagggt gaaggctcgg tcggacgggt tggtcagcac
    12901 ctcggcgccc ttgaacaggc aggacgcggt gaagctggcc gggtcgtacg cgatcggctt
    12961 gccgttggcg tccagggccg cgctgaccac cgacttggtg acggtgaagc cggcgaagcg
    13021 gaactcgttg gtgaaggccg ccggggtggt cttgttggct tccaactggg tcagggcgac
    13081 ctgccgggac gccggttcga cgctggtcgc gccggccgag ctggtctcgg tgaccaggca
    13141 gttcgacccc ggcgggatcg gcccgagctc agccgaggcc aggctggtct gcccggcgga
    13201 cggcgtcagg gtgacgtcct tgctgaacac cgatcccgaa ccgcccaggg tgcaggccac
    13261 cgtgaaggtg aacggcccgg tgccccaggt ggtggcggca tcgccggtga tcgtcttggt
    13321 gatctgcacc ttcgcggtgc tgaaggtgtt ggtcagggtc tggcccttgg tctcgccggc
    13381 ggcgatggtg accccaccgg tggaggtgac taccggggct ggctgagtcg gggtgccggc
    13441 ggggtaggtg ataccggtct cggccgccgc gcaggaggcg ccggtgggca ggcccgccca
    13501 gatcaccgag cccggtccgg tgacggtctt gaccggattc ggcaccggga acgactcggc
    13561 ggttccgtca ccgttgaagt ccttcgtgca gctcagggtg aactcgtaga ccgtgtcctt
    13621 ccagggcgcg agcaggccca gtccgtccgc gatcgccttg gtgacctgca ccgaacccac
    13681 ggtgaaggtg ttggtgatcg tcaccggctg gttggtggtg cctatggtca gggtgctcgg
    13741 cacgacggtg gtcgcgttcg cgccgccggt cttgggctcc gacaccgtgc agttcgcccc
    13801 ggtgggcagg ttgcgcagct tccaggtatc gccgccggtc aggtccttgg tgccatcgta
    13861 ggcggtggcc gcgggagtac cgacgaccag gtcgtcgtcg aaggtacaga ccacccggac
    13921 ggtgaaggga ccggcgcccc acaacccggc accggctcca gtgacgacct tgctcaggtc
    13981 gaggccaccg gtggtgaagt ggttggtgaa cgcggcggtg gtggtcgccg aggtgccggt
    14041 ggccaggttg aacgtcgcgg tcgggctgga cggggaatcc accagcgtgc tgccgttctg
    14101 ggtcaccttc accccggtgg acgcggcttc gccggtgccg gtctcggtca ccgtgcagga
    14161 cgccccgctg ggcaactgct cgaaggcctt ggtctcccag gtcttggcga cgacgtggtg
    14221 cagggtgaac gtctggttca ggatctggtt gccgttgaag gtgcagaccg cgcggtagtc
    14281 gaagctgcgc cggtagatca cctggtcacc ggacgcgtcc agcgcgccgc cgttgtcgac
    14341 ggtcttggtg accgtgaagc cggcgtacag gaaggtgttg gtgaagttcg ccgcggtggt
    14401 cgtccccgac ggcaggttct tcagcacgac cgtggtgcct ggcgcgacgc tggtggcgcc
    14461 cgccgagttg gtctcggtga ccgtgcagtc ggcgccctca ggaatcgggc cgatggccgc
    14521 cgaggtcagg ttgggcgccg tcagggtgat gccggtcctg gtgaagacgt agccggcggt
    14581 tcccgcgagg gtgcactcga ccttgaacga gaaggtaccg gtaccccact tggccgccgc
    14641 ttcgccggcc agcgacttca cgatctggac gttgccgaac ttgaagtcgt tggtgaccgt
    14701 gaagctggcc gtggttccgt tgccgacgac cgcgtcgccg ctgtaggtga ccgtgggatc
    14761 gggctgtgtc ggggtgagcg gtgggtactc gatggtggtc tcggtcgccc ggcaggtggc
    14821 atcctgcgga aggcccgtcc aggtggccgc gaagccgggg gccttgagca ccttggtgtc
    14881 gccgccgacc agagccatgt tcagcgactc gggcgtcccg tcgacgatcc gggtgcagct
    14941 cagcttcatg gtgtagctgg cgctggccca gggctcgatg gcggtggcgg actggccgtt
    15001 gaccagcagc accttgctca ccgtcaccga gccggtgttg aaggtgttgg tgaccttcgt
    15061 ggtgacggtg gtgtccttgc cgatggtctg gttcgggacg tcgatcgtcg acgcgttggc
    15121 gccaccggtc ttgggctcgg agatggcgca cttggcgccg tagggcaggt tggagatcgc
    15181 gtccagggtg tggatgccgt tggcctcggt gacggtgaac gggaagtcgt tggcgtagat
    15241 gaccgtcccg gtggcggggt agtcgaccgt gcagctcacc cgaagggtga acggcgccgt
    15301 accccagacc gtgccggacg ggctcagctg cttcaccaac tggagcttgc cgacccggaa
    15361 ggtgttggtg aacgcagcct tgtttgcggc cgtctcgccg ttgatgacgc tggtgtcgcc
    15421 cctgaccagg gcgaactgcg aggtggccag ggtggcgccg gtgaggtcgg gggtgatgcc
    15481 ggttcgggtg atccggtagc tgacatcagg ggtcagcggc atctgggtct cggtgactcg
    15541 gcagccggcg ccgacggcga gcttgtcggc ggtccaggtg ccccaggggc cattgccggt
    15601 gccagtgagc tggaaggtct ggtcgagcac cgggtaggtg gtcgacgcgc cgttgctgat
    15661 cgtgcagacc gcccggaagg tcaccgtggt cgggtagctg atggtcacgc cggcctggtt
    15721 ctgggccaca gccccggcca ccgtcttgga gacggtcagc ccggcgtact cgtaggtgtt
    15781 ggtggcgtcg accgccaact cctgaggctt gctcggatcg gcctgcggaa cgacggcctt
    15841 agtgccgtcg taggcgctcg gggtgacggt ggtggacgtg gctccgccct tgactccggt
    15901 ctcactggcc cagcagctgg tgcccaccgg gaacagggtc ggggtggtgt cgttgggcac
    15961 catggtgtga ccggcgccgg tgatggtcac cgtggtggtc gtccggttgg gatcggtggc
    16021 cgacttgcca caggtcacgt tgaaggtgaa cttggcgttc ttggcccagg gctcgtcctt
    16081 ggctgcgcca gcgagcgcct tggtgacggt cacccgcccg gccgagaagt agttggtgaa
    16141 gttcgtggtg aagacgtcgt tgctgctgtt cggttcgacg attgagacgg tctgcgggcc
    16201 gggggtcttg tcggcgtccg ctccaccgct gttggtctcg gtgaccaggc aatccgagcc
    16261 gaacgggatc ggcccgagct cagcggaggc gagggtgccc tttggcaccg agaccgagcc
    16321 gttggtataa acggtggtgc cgcccatcgt gcaggtcacc gagaaggtgg ccccggtgaa
    16381 gaaggacgcg cccgcaccct tgagttcctt ggtgatcttc agcttgccgg cccggtagtt
    16441 gttggtcacc gtgaacgctt cggtcgagcc gtccggcacg ttcagcgtgg ccggagtgat
    16501 cgtcttggtc ggcgtgggct ggccggtcac cccggtgatg tcgcccacgt tctcgtccac
    16561 cgagcagctg gccgcggccg gaaggtcctt ccaggtggcc gtccagtcgt cggccgcacg
    16621 cagcgtcgca gtgcgcgtga tcactttgtc tgcgccgtcg acgttgcgct tgcaggtcag
    16681 ggtcaccggg aactcaccgg acgaccacgg agcgagcgcg gacacgttgg tgccgttggc
    16741 cagcaccttc ttggtcacgc tcacctggcc gagaccgaag tagttctgga caccaatctg
    16801 agtgggcttg gtgggatcgg tgcccacggt gaactcactg ttggtgatcg tgttgctgtt
    16861 ggcaccgccg agcttggtct cggtgaccgt gcagctcgcg ccgctggcga gcttggacac
    16921 cgtccaggtg ggggtggcct tggtcagcgt gtgggacgcg gtgaacaccg gggcggtgat
    16981 ggcgtccagg tgggtgcaga ccagcttcac ctcgaagttc tcgttgcccc acagctcggc
    17041 gcccggtccg gtcacgctct tggtgatggt ggccgcaccg acggtgtagg tgttggtgta
    17101 gtcgaccttg gtggccgtct cgtcaccggc gaccagggag aacgtggtct tggtggtgcc
    17161 ggaatcgacg gtggtcgagc cctgggtggt gaccaccttg gtgctggctg cgccggccgc
    17221 ataggtctcg gtgaccgagc aatctgcacc ggccggcagt cgggcgaact tcctgctgcc
    17281 accaggggac aggctgaacg acttgtcggc gtcggcgagc acttcatggc ccagcagggt
    17341 gcagctggcg gtgaacttga agtccttgaa cgccaccggg gtgattccgt cggcctggag
    17401 cgcggccggt ccgtccacgg ccttggagac ggtgaagcca gcctcgcggt aggtgttggt
    17461 cacggtgagc ttgccggcgg gcgtgcccgc gccccagccg gtcagcacgt tcgcaatccc
    17521 ggagtagtcg cgcagcgcct tcactttggc cggggtgacg gcggcgtccg ctccttggct
    17581 ggcgtcctcg acgacggtgc agtcggcgaa cagtggcagg ttcatggtgc ggtcggcgtt
    17641 cagcgagacc ggggtgccat tggccggcag gctgacctgg cgggggttcg agccggggat
    17701 ggtgggcagc ttggtgttgt ccagcgaggt gcaggtgacc gtcgccgggt agctggacgg
    17761 caacagcccc gcccagctga cgtccgcagg ggtgaccacg gccttggcca ggtcgagctg
    17821 gccgctcgcg atggcaacgc ccaccttctt gggttcggtg atcagcgagg cccgccacgg
    17881 gaaggtctcg gagtacgcgg tgcgggagcc gactgccacc gagttccagg cgatcggctc
    17941 gacggtgttg gtgtcggcca ccggggcgta ggccggggtg cgggtgtcga agctgacgta
    18001 gaaggggtcg cccggcctca ggccgtccgc cttgttgtcg aacttcagca gcaccttgat
    18061 cgccttggcc ttggccaagt cgccggcggg ggtcgagccg tcgaaggcga tccaggtggt
    18121 gccgcagttg gcctgcccgt ccggcttggt gtcgttgagg atgtcgttct tgttgcagct
    18181 cgggctcggc gcggtcgtgg agtagtagta ggtcagcttg tgcggtgcag cgctggtcag
    18241 accggtgacc ttcaggttgc ccaggaaggt cggggtgaac cgcgacttgc gcgcggtgtt
    18301 gacggtgacg ccggtgtcgc ccggtgcggg caacacgtcg atcgcgctga ccacattggc
    18361 cgccacgttg ccgttgttgg tcagcgacag ccgccaggtc tccaccccac ccggacgggt
    18421 gatcggcgcg cagttgccgg tggagtagcc gtcgccgacc tcccgctgcc gcttgcactc
    18481 ctcagcgttc gctgcgccta ccgaaatcac gcccagatcg tcgtagttga cgctgtcgga
    18541 cgcagcggcc gggtcgccgg ccgcgctgcc cttgaccgcc ttggtcacgg tgagcggggt
    18601 ggacgccgcg gccaccacgg tcgtgctggc cgcgcactcg gccacgccag tggtgatcgc
    18661 gtcctgttcg atggcgtcct tggtgtactc gcagcgctcg aaagcgcgat cggaggtaac
    18721 ggcgagggtg ttggccacct cggtgccggc ggtgagccgg tcgcggaacc gcaggttggc
    18781 gatgatctgc agcacgccgt cgggggccag ccggaattcg gtgggaacac ccagcgccag
    18841 cttcccggtg tcttcgtcga gggtcaaggt gaagccggtc tgggtgacgc cgttgacctt
    18901 gacggtgtac ttcgggtcga cgtcggcgaa gaccagcttc gacttgccgt cgtcgaccag
    18961 gccgatcgtg tcggtcagct ccagcccggt catcgtccag tcaccgatgt tggtgacggt
    19021 gaaggtgtag gggatggtgg cgtcgaggtc gtagcgcggg gcttcggtcg cctgtccgcg
    19081 tccgggcgtc ttctccaccc tgatctggtt cacccgatgg ttgagcgtgg tggtggccac
    19141 agcctggtcg tcggcgatcc acgggctgcc ctggttgcgc caggcggcga ggccgtcgac
    19201 gcgcagctgg tcggtcgtga cgccctgggt cgtctcaccc ggagccttgg ccagaccggg
    19261 cagggtgctg ggcaccgggg tggtgctgcc gccgtcggtg ccgtaccgca gcagcgtccg
    19321 ctgcttggtg gtgaagctga tgctcagctt ggggtcggcc ggacgctccc actgcagcac
    19381 ggtggtgccg tcggccgcga cccgttgggc ctcgaagcgc aggccgagaa cctggtcggc
    19441 ggtgattccg gccggcagcg tcggggtgac ctgctggttg accgcggtcg accagtcgcc
    19501 ggtgcgccaa caggcggcga ggtcggtgtt gccttcgcac ttggctacca gggtggcgcc
    19561 ctcaaggctg taggtgacgc cggtgagcac cgacagccgc agctggttga ccgggctcgg
    19621 cagcgtgacc ggacgcagta cgtccaggtc gaaggcgttc cagaaggtgg gcgcgtcgtc
    19681 ggtaagagtg agcagcttcg tgcgggcggt gctgaagacc ttgtccgaat ccctttggca
    19741 gcgggagccg tcggcgtcgc acggctggcc ggtgagggtg accgtgtagc cgttcgcctc
    19801 gtcctcgtag cgctgtccgg gtgagatggt cttgcccgcc tggaccgcgt agtcgggctg
    19861 gcgaatgtcg atctcgccgg aggcggtcga gctgggcttg tcgcaggtct ggctctgatc
    19921 gtccgcgcag ccgatgccgc ccggactgac cagggtggct tgggcgatgt tggcgacgtg
    19981 gttgatcggt tccaccgggg cggcggtgct gcgcagcgtg ccgcgcaact ggtaggtgag
    20041 caccagcttg gcccgcccat tagcggggat cagcggcttg gccggatcct tcaggttggc
    20101 ctcggcgccg tacttcacga ccacgccgac cacgtccttc aggtcggccg ccgtcagggc
    20161 ctgggcttcg gcgtagctcc ggaccaggtc actgccatcg gccagcgcca gcagcacggt
    20221 gacatcgtcg ctggataggc ctgcgggcag ctcggcgaac tggagctggt agaggttcag
    20281 gtagtcgtac cagggcgtgc tggagctggg cttggggtcg gcgatgtcga actcgggaac
    20341 cccgctggcg gtgttgttcg tcgcggtcag ggtcgcgctg accagcggga acgcgtccga
    20401 atcggctgtg tcctgcggca ggccgacggc ggtctggtcg agctgcttgg tcaggctgac
    20461 gttcagcggc tggtcgtaga tggtgatctg ggcggagtcg agggtggtga tccgcttggt
    20521 gccgtcgtcc agcggggtgg tcagctggcc ggtcgcctcg gcgttgttca gcaccacgcc
    20581 ggcccgcccg gagttgtact tcaccgaatg gctggtgccg agcacgtagg tgctggagtc
    20641 gctgcgcagg gtgcggcgca gctgtaggtc cagacggacc tggcggtcgt gggcgttgcc
    20701 gttggtggcc accgcgccgg tgtcggagtc ggtgccggga gcgaaggtca gccggacggc
    20761 ggtcgcgctg gcccgctcgg tgtcgttaag agtgtagccg ccgaaggtgc cgtcacaccc
    20821 ggcggcgcag gccgccccgg tgatgttcgt ccagccgctg ccggccaggt agagctcgac
    20881 cttggccacc ttgtccttgg ccatcagcgg gtcgatcgcg gcggtgatcg gttcgatccg
    20941 caccaggtcg aaggcgtcga agacggacgc gctgacgtcg ccggtgtgcg ggtcgtcgac
    21001 gacctcgact gtgctcatgc tcaggccact ggtgccccag cggatcgctg cggtgcggat
    21061 gtcgtccgac agcgccgaga cgctgacgtt ctcctcgtag gtcttgtcct cggggtcgag
    21121 ccagaacttg tcgaccagat cgacgttgcc gccaccgccg ccaccggtcg ggtgcagggc
    21181 gagggtgtcc ttggcggtat cggtgaccaa cttttgaatg gcctggtcat tggccacctc
    21241 ggaggtgacc aggttgctga gctcatcgac gcccacggtc gccgaggtgc ccggagctga
    21301 gcgcagctgc gcgcgcagcg cgacctcgaa gcggggaacc acggtgaagc cggccggcag
    21361 gtcggagccg tcggtgccga cgaagcggaa ccggacgccc tgggcggccg cgcgctgcgg
    21421 atcggtgagc ttgtaggacc agtcggtctt cgggccgacc accggggcgg tcagcggctg
    21481 ccaggtggtg ccgtcgaagt actccgggta catggtggcg ttgctgggca catcggtggc
    21541 gacgatccgg cgcaggtcga agtggtccca gaaggtttgg tcgttgtcgg tgatcaccag
    21601 tcgggtggaa ccgatggtcg actcggaggc gaccgagccg gtcacctcag cggtggcggt
    21661 ggcgccggcg gaggcccaga tccagtcgcg gttgaaggcc ttcttcacct cggtctggac
    21721 gaacttgggc agcagggtga tctccttggt gacggtggcc gacttggggg ggccggctgc
    21781 gttggacgcg gtcgcagaga tcgtgttgga ggcaaccgtc ccgctctcct cggttaccgg
    21841 cccggcggtg accgtgaagg gcagccggac cagagcgttc gactcgatcc cgtccgcatc
    21901 ggcggaggtg aaggtgacgc tgaacccggc gaccggctgg gtcggcgccg gcagcgtgtc
    21961 ggcgtgggtg gtgctgaggg tcgcggtggt gccgtcggtg taggtgtagg tgatctcggc
    22021 cttgctcgcg ccgtccggcc aggtgacctg cgcgtccgcg ctcggatcgg tgacgaagcc
    22081 gccgaagatc aggccctgct cggccaggtt ctcgtgatcg gcggccggct cgttgatggt
    22141 gatcgttgac gtcggcatca ccccggtggt ggccttcagc gtcaccgtgg tcgacttgcc
    22201 gaccaccagt gtggggccgt cgaagctctt gctcgccgag atctgcaccg actgcttctt
    22261 gaagctgatc gttgcgctgg cttcgtcctt cgcggtctcg ctgcccttgg tgaccgtgct
    22321 ggtcagggtg ttggtcagcg tccggacctc gccctcgtcc agcccatcgg cgatgttgcc
    22381 gcggctgacc gtcttcagca cgatcttcgc cggggagccg gagggcaggc tgccgccgtc
    22441 cttggtgaag gtgaaccgga cgcaggtgat ggtggccggg tcgacgccgc tggggatcag
    22501 ggtgctcggg tcgtccggga tgggcaccgc tgcgccgttg tgccagtcgg tgccgttgaa
    22561 gtagtcgaag ctgacccggt cggcgccgcc gggggcctcc aggctgtcga ttccggtcag
    22621 ggccaggtag ctgaacgggt tgccggacgc ggtcgggtcg gtgggatcgg ccagcacgat
    22681 cgagtcgacg ctgacgttgg ccagactccc gccgccgagg gtgatggtgg ccgggcgtcc
    22741 ggggatcgag ggctgggtgg agttcggggt gatcgacttg gtggcggacg ccttcagctt
    22801 ctgctcgatg ttgagcttga ctgaggcggt gtcggagacc ttgtcggcgt tgtcggcggt
    22861 cgcgtcgaag gtgatgtcgg cggtgttgcc gttgtagtcg gccgaggcgt ccgcgggcag
    22921 cgtcgccttg accgtgacca tcacgacctg gccggcgtcc agttgtaagg cgtggtcgtc
    22981 gttgacgaag gtgatggtga gctgctgccc gtcgatggca acagtgccga ggttcgggct
    23041 gaccgtgatt gccggatcgt cgtagaccag gggcgccggc agcttcgccg agaccacggc
    23101 gttctggcag tcgttggcgc tgcactgcag gttgaccgtg tagctgaagt tctcgcccgg
    23161 cgagatcgtc ggcacttcgc cctgatgacc ggtgacgaag gcctcgatgg tcatctccgc
    23221 atcgatctcc cctgccgagg ctgtgatggg cagcccaaga gccagcagaa gcagactcat
    23281 gattacgctg agagtggccc tgagagaagt tcggcagcgt gaacgagagc gtgggggcat
    23341 attgaactgg gccttcgttg cgggaggttg ggtcttttga cgggcccaat aacgatagct
    23401 agctacgtat tactgcggaa cggtcccaag cgtcaagaag atcacaatca gcgcgaactc
    23461 gcgacccttc cttgagagtc gggaacgcgt aagggccggc cgggaattcc cggccggccc
    23521 ttaccgctga aggtcaggcg ctcaggcgct cctgcgtctt ctcggagaag acgtgcatgg
    23581 tctccgcctc gacggtgttc agcttgacga cggtgccgcg ctgcggtggc gtccgagccg
    23641 agacacgggc aacgatctgc tgagcggtga gcttctgctc ggcgtccagg cccgcgagcg
    23701 tgccgtagac gtaggcgtcg gcgccggtct cctcgaccac gtccacctca agaccgatgc
    23761 cggtttcggc gatccggaag ttctccggac ggatgccgag caccaggctc tcctcgccgg
    23821 cggccttggc cagcacctcg cgggcgaccg gaacgacgta gtcgccgatc tgcacaccgc
    23881 cttcgacgat cttgccgctg atcaggttca tggccggcga gccgatgaag cctgcaacga
    23941 acaggttggc ggggcggtcg tagagggtca gcgggctgtc gacctgctgc aggatgccgt
    24001 ccttcatcac cgcgacgcgg tcgcccatcg tcatggcctc gacctggtcg tgggtcacgt
    24061 agacggtggt gacgccgagg cgggtctgca gggcggcgat ctgggtgcgg gtcgagacgc
    24121 gcagcttggc gtccaggttc gacagcggct cgtccatcag gaagacctgc ggctggcgga
    24181 cgatggcgcg gcccatggcg acgcgctggc gctgaccacc ggagagggcc ttcggcttgc
    24241 ggccgaggaa cgactccagg ccgagcagat gcgcggcttc gagcacgcgc tggtcgcgct
    24301 cagccttggg aacgccggcc atcttcaggg cgaagcccat gttgtcagcg acagtcatgt
    24361 gcgggtacag cgcgtagttc tggaagacca tcgcgatgtc gcggtccttc gggggcaggt
    24421 cggtgacgtc gcggtcgccg atcaggatct gacccgagtt gacctcttcg aggccggcga
    24481 gcatgcgcag cgaggtggac ttgccgcaac cggacgggcc gaccagcacc atgaactcgc
    24541 cgtcgccgat ctcgaggttc agcttgtcga ctgcggggtg gtcagagccg ggatacaccc
    24601 gggtcgcgtc cttgaaacta actgtggcca tgccaagtaa tcctttccac gggcaggtac
    24661 gtgcccgacg atccgtagtg gaagtcgcgc cgcgggtgcg gcgcattaag tgtgccatgg
    24721 ttcacgcacc tcgaaccaga agatcgtttc aatcgttatc tggttgggat cggacgcctg
    24781 cccgccgggt agtctccccc gggtgagcga cgtccagcca gagaccgccg gtgaccagcc
    24841 cgccgctgag cgtgaatggt gggacgaccc gtcgctgccg tggcggcaca agccgaccag
    24901 ggccgacatc gcctgcttca gctggctggg cgcggtggcg atctactcga ttgtgatctc
    24961 ggttctgcgg ccgggcctgc tggcctcggc gccgcacgtg ctcgcgtccc tcggctcgtg
    25021 gtcggggacg atcctgatcg gcgcaagggc ggcggtcggc gacccctggt ggctgctggt
    25081 ctgggtgctg ggcacgctcg gcatgatcaa gttcgactgg atctactggt gggcgggcaa
    25141 actctgggga cgcagcctga tcgaggcatg gtcgggccgt tccgagcgcg cgcgccgcgc
    25201 caacgcctgg gctgagcggt tcgctcgcaa gtacgaggtc ttggccattg cgatcacctt
    25261 cctgccgatc ccgctgccgc gcggcgtgat cttcgccgtg ctcggtgagg ccggaaccag
    25321 cctgcggaag ttcctgaccg ttgccgcgat ctcgtctttc atctcgaccg gcggctacct
    25381 tgcgctcggc tactggatcg gggaaccggc ggtggccgtc atggacgcct acggccgcta
    25441 cctgtggatc gtgtcgctgg tgatcctggt ggtcgtggtc tacaacgctt ggcgcaaggg
    25501 agccccgaag ccggtctagc
//
LOCUS       DAOPRQ010000037        25102 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_1618_length_25102_cov_11.730028, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000037 DAOPRQ010000000
VERSION     DAOPRQ010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 25102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25102
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1618_length_25102_cov_11.730028"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1964
                     /locus_tag="PKJ61_06465"
     CDS             <1..1964
                     /locus_tag="PKJ61_06465"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008357776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HOC13503.1"
                     /translation="PRANAEAVLAQSREAADAGACLAVFPELALTGYSIEDLLMQDTV
                     LQATTAALDWLVPQTQDVGAIIVVGAPLVSLNRIYNCAVVIHRGVVLGVVPKSYLPTY
                     REFYERRQVAPGDDVTGEIRIAGQDVPFGSDLLFAAEDVPGFVLNVEICEDMWVPIPP
                     SAEAALAGATLIANLSGSPITVAKAEERKLLCASGSARLLAGYVYSAAGEGESSTDLS
                     WDGQTLIYEAGTLLGESERFPDGPRMTIADVDLGSLVQHRLRQGTFDDNRRTHAERTG
                     EYRTVTFELDPPSGDLGLQRKLARFPFVPADPTRLALDCYEAYNIQVSGLEQRLREIG
                     QPKIVIGVSGGLDSTHALIVAARAMDRMDRPRTDILAFTMPGFATSAHTKGNATALAK
                     SLGVTFAELDITGTARTMLDEMNHPFAEGQPVYDVTFENVQAGLRTDYLFRLANQRGG
                     IVLGTGDLSELALGWCTYGVGDQMSHYNVNGGVPKTLMQHLIRWVISSAQFDDKVSAT
                     LQAILDTEISPELVPVTEGEVPQSTEASIGPYSLQDFNLYHVLRLGMKPSKIAFLAWH
                     AWSDASAGEWPEGFPHDERTAYDLATIKKWLEVFCKRFFGFAQFKRSALPNGPKVVQG
                     GSLSPRGDWRAPSDTSAALWLEDLANIPS"
     gene            complement(2016..4853)
                     /locus_tag="PKJ61_06470"
     CDS             complement(2016..4853)
                     /locus_tag="PKJ61_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016456336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HOC13504.1"
                     /translation="MLDSEDPQSASPTPDEPNQPPRRRRAASRPAGPPAPVETVVAAP
                     AEAASAPQPTVAAELSGTAQSTETTTPAADAPSPAADVPAEPPQKRTRRARTKPAAAE
                     PVLPLAEPTEPAEPAKAAEPSAAEAPAKPVRRRAVTKKTKAAPVVAEAATETGHSTAA
                     APVAASATPAEADANAPADNDAAADAVAASLTDLAKGRRRTRRPALAESFGLGEEATP
                     DEVLDSLAAAIGGLLEPAADLEEEAEEEPLDEAEELDEAEESEEDAEGMEGDEGQPRR
                     RRRRRGGRRRRRGAGEAAVDSEDAGDEDATVAEPADGENDGDTEGESDEATAEGTSRR
                     RRRRRRRGEDTGEPEDESIDVVVRVREPRRRTSSASDEVTGIEGSTRMEAKKQRRREG
                     RAAGRRRTPILSESEFLARREAVDRKMLIRQAEDYSQLAVIEDGVLVEHYVDRASATS
                     MIGNIYLGRVQNVLPSMEAAFVDIGRGRNAVLYAGEIDWDSFGVTGQDKKVEKVFKSG
                     QQVLVQVSKDPVGAKGARLTSHISIPGRYIVYAPGGHLSGISRKLPETERKRLKGILD
                     ELIEPQASVIVRTAAEGASEDELVRDVNRLKAQWEVLEKKVKAGQAPQLLYAEPDLTV
                     RIVRDLFTEDFGSLVIDGNGQADDAYDTVDAYVRHVAPHLAERMEKWDRTSKGDIFDA
                     FRVHEQVAKALERKVFLPSGGSLIIDRTEAMTVIDVNTGKFTGTSGNLEATVTSNNLE
                     AAEEIVRQLRLRDIGGIIVIDFIDMVLPANRELLLRRLVECLGRDRTRHQVAEVTSLG
                     LVQMTRKKIGTGLAEAFTVSCETCKGRGYHVHDAPVASQAPADGGDRESGRRTSRGGS
                     SASGGSRRSARTPKDAPKPKPAEPVPAEVPPVEVAPPVEPVPVEVGPPVDPVPVEVGP
                     PVEPVPVEVAPAEVDAPAAEPVVTADAPAVE"
     gene            complement(5096..5794)
                     /locus_tag="PKJ61_06475"
     CDS             complement(5096..5794)
                     /locus_tag="PKJ61_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008358521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HOC13505.1"
                     /translation="MSARRQPEQQAPPVQKLRVQYAKRGRARFASHRDFGRAFERALR
                     RAEIPMAFSSGFSPHPRISYANASPTGAATEAEYLELGLAEVREPDAVRDQLNAAMPD
                     GLVIVKVVVASPGALAERLTTSAWQVDLPDVVEAELSTAVAALLAADSFEVQRMTKTG
                     LRTFDVRAAVISLTASANGLNLVSAIGEPLVRPDDVLNALRQLAPELPSNALITRLAQ
                     GTWDGERILDPLAD"
     gene            5855..6343
                     /locus_tag="PKJ61_06480"
     CDS             5855..6343
                     /locus_tag="PKJ61_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012922434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOC13506.1"
                     /translation="MIELRLAEPADYPAVGELTAQAYLADGALPADDPYYEVLRDAAA
                     RAESGELWVAVEADSLLGTVTWCPPGSRFRELAHPNEGEFRTLAVAVAARGRGAGRLL
                     VEKCLQLATDAGSTAVVISTAEWMTTAHELYRRLGFVEAPERDWSARPDVRLRAFVRP
                     LN"
     gene            complement(6385..6828)
                     /locus_tag="PKJ61_06485"
     CDS             complement(6385..6828)
                     /locus_tag="PKJ61_06485"
                     /inference="COORDINATES: protein motif:HMM:NF037170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOC13507.1"
                     /translation="MSLTFQVTLDCADPHVLADWWAETLGWEVEPTDEAFIRKMVTAG
                     YATEAETRVYKDQLVWATGAAIRVPDAPEGSPCRRVLFQLVPEPKTVKNRMHLDVRLG
                     VGADLDAARDALVARGANFSHEASQGPHRWYTLTDPEGNEFCVSE"
     gene            complement(6825..>7778)
                     /locus_tag="PKJ61_06490"
     CDS             complement(6825..>7778)
                     /locus_tag="PKJ61_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008361414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HOC13508.1"
                     /translation="VGYSDVILTGRAFASRKGQRVDANTELLAVGAANLANGLVGGFP
                     VSSSASRTVIGDSAGSQTQLHSLVAAAMVVGSIYLFSPVLAAFPQAALAGVVIYAGLR
                     LVDLAELGRIARFRGSELALSLITFVAVLATGLLNGIGIAIGLSLLDLIRRIAHPHDG
                     VLGYVPGVAGMHDVEDYPQASQVPGLVVYRYDSPLFFANADDFLTRAMEAVRAADPPP
                     LWFVLNAEANVEVDLTAVDTLEELRRTLHADGIVFAMARVKMEVRDQLAAAGFVEAVG
                     EEHIFATLPTAVAGYAAWHEDVLGTRPIGVPDIPPQPKENR"
     assembly_gap    7779..7878
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7879..8740)
                     /locus_tag="PKJ61_06495"
     CDS             complement(<7879..8740)
                     /locus_tag="PKJ61_06495"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HOC13509.1"
                     /translation="MSTAAARRWRRWAPGLGVLLGYDRVWLRGDLLAGVTVSAYLVPQ
                     VMAYAEIIGMPPIAGLWAVLAPMLVYAILGTSLQLSVGPESTTALMTAAAIAALVGEV
                     DLQRRTEVAALVAIAVGVVLGVGWLARLGFLANLLSKPVLVGYMAGIGLLMVISQYAT
                     LTKLVIVGDNPVAETGSLLAQLGSVHLPTLTLGLVVAGLLFALHRWAPKLPGPLLVML
                     LAAAAVHFGGLGAYGVEVIGEIPRDLPVVSWPNLNGIDPLQLTLAAVGIAVVGYSDVI
                     LTGRAFASRKG"
     gene            complement(8770..10035)
                     /locus_tag="PKJ61_06500"
     CDS             complement(8770..10035)
                     /locus_tag="PKJ61_06500"
                     /inference="COORDINATES: protein motif:HMM:NF020075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HOC13510.1"
                     /translation="MLRTHRVRSLAVLVALALLALVMAPAAPAEAAKKAPAKFVIAGS
                     GAGHGVGMSQYGAYQLARTGSGAEQILQYYYPGTTIGWTNNNPRTVKVQVLGPPVDKR
                     KKATIKVKGGGFTVVDTAGRVLLGTSSGKITLKVKGSGVTAKAGKKSLTASRLTVTTT
                     GTATVTGAQGSYHYGNLQVTTIGKRLNVVNEVAMNTKYLYGLDEMPASWGNYGGAEAL
                     KAQAIAARNYVIKRVIKLRETLGVDTGDPACDCHLFDDERSQNYTGWKKTGSSANQPW
                     VDAVKSTITETGVLILRDPSAGFAETPYFASTGFAAGAGTARNSDVFKADPLSYLPGV
                     ADPYSALAPRNPYKSWKRTLTQKQAAKVFNLKSITKIEVAETYSGGLMKTLTATATNG
                     RTVTLSKTSRAWMEVLAVPSPWLTSIKPK"
     gene            complement(10216..12162)
                     /locus_tag="PKJ61_06505"
     CDS             complement(10216..12162)
                     /locus_tag="PKJ61_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007078313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HOC13511.1"
                     /translation="MNPSVESLYPRLEPLLARVTKPVQYTGGEVNSVSKAFDSVEVRW
                     ALMYPDAYEVGQPNQGLAILYEILNERDWLLAERTFSIWPDLAKLMREHGLPQFTLEN
                     HLPVAAFDLFGVSFSTELGYTNLLEALDLAGLPLHAADRREQDPIVIAGGHAAFNPEP
                     IADFIDAAVLGDGEQAALTISEIVREWKASGRPDGRSGLLARLAASGVAYVPSLYDVS
                     YGPDGTIASVVPNRPGVPAKVAKHTLMELDEWPYPAKPVVPLAETVHERYSVEIFRGC
                     TRGCRFCQAGMITRPVRERNIETIGKMVEGGLAATGLEEVGLLSLSSADHSEIAPVTK
                     QLADRYDGSFVSLSLPSTRVDAFNIDLANELSRNGRRSGLTFAPEGGSERMRKVINKM
                     VSEDDLINTVAAAFSQGWRQVKLYFMCGLPTETDEDVLGIADMAAHVIEAGRRAAGTR
                     DVRCTISIGGFVPKPHTPFQWVAQADPDTIDRRLRLLKDKIREDRRYGRAIGMRYHDG
                     KPGMIEGLLSRGDRRVGAVIEAVWRDGGVLDGWSEFFSFERWTAAAERALTPLGIDLA
                     WYTTRERPQGEVLPWDHLDAGLDRDWLWQDYQDALYGAEVDDCRWSGCYDCGVCPQFG
                     TEIQIGPTGRSLLPLTVVTRPEAS"
     gene            complement(12623..13303)
                     /locus_tag="PKJ61_06510"
     CDS             complement(12623..13303)
                     /locus_tag="PKJ61_06510"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HOC13512.1"
                     /translation="MGTEPVEKLDAPGGLKWLRARIRARSGRTLVGIDGVDGAGKTTF
                     ANELADLLRESGIEVIRISMDHYLNPQTKRYAQGRSSAKGYFEDSYDYERFTDEVLEP
                     LGRDGSGRYRTAAYDLDSESEVHSPWRVAPDEAVVIVDGMFIHRDELCSSKRNKIWDL
                     SVWLDVPFDESFHRMSERDHKLSADPQDPGNARYYQGQLLYLASCDPAKRADLVVENA
                     APHSADID"
     gene            complement(13410..14348)
                     /locus_tag="PKJ61_06515"
     CDS             complement(13410..14348)
                     /locus_tag="PKJ61_06515"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOC13513.1"
                     /translation="MPVKTAALPVVRREYHEFFRAPRFRWWRPLLGLAMFVAVWFVGT
                     LVVTVAALAYDAFTGRTNPGELTTEVPMTPALFTANNIGLGLAIPIAGLTAWAVFGQR
                     PRWMSSILGGFRWGLFWRFSLVAAGIFLVGLAVSFAIGGEGGSEMRWNADSAFLIAAI
                     LLTTPFQAAGEEYGVRGFLARSIGSWFGARRVGLVVATVITSVVFMILHAADNIWLNI
                     YYFSVGVVCSVLVWRTGGLEAAVALHVFNNLISEVTLPFVPLDEVFARGPNDAGPEVL
                     VQLVFTFSVMAAMLWIAKRQRLQSAAAPGAQPVTGP"
     gene            complement(14501..14917)
                     /gene="ndk"
                     /locus_tag="PKJ61_06520"
     CDS             complement(14501..14917)
                     /gene="ndk"
                     /locus_tag="PKJ61_06520"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004811748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HOC13514.1"
                     /translation="MTDLQRSLVLLKPDAVRRGLAGTILARYEAKGLSIVALELRQVD
                     AGLADRHYGDHVAQPWYPPLREFITSGPTVAMVLEGPSAIEVIRLMNGATNAVVAAPG
                     TIRGDYSLSNRENLVHASDSPESAEREIGLWFPNLV"
     gene            complement(15006..15353)
                     /locus_tag="PKJ61_06525"
     CDS             complement(15006..15353)
                     /locus_tag="PKJ61_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015070053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4233 domain-containing protein"
                     /protein_id="HOC13515.1"
                     /translation="MGLDRTNPMGRVMLSILAFEAIVFGLAIAGMVQVDAVSVPVAFG
                     IGLGAVALAVLAAALLRRPLGYPLGWLTQVVAVGMGFVTEMMFAVGGMFALLWAVCFV
                     LGRRIEKTHRASA"
     gene            complement(15373..16680)
                     /locus_tag="PKJ61_06530"
                     /pseudo
     CDS             complement(15373..16680)
                     /locus_tag="PKJ61_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013160744.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
     gene            16745..17296
                     /locus_tag="PKJ61_06535"
     CDS             16745..17296
                     /locus_tag="PKJ61_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020388808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HOC13516.1"
                     /translation="MTETPAVGALRRPWTEWVTLACRLGLGGVLLVAGLLKVGHLDAS
                     VQSVRLYQLLPWEITAFVGSALPIIEIAMGLLLITGTFTRISAILGGLLMLAFIIGIA
                     SVWARGISIDCGCFAAGGEVDPSATQYPLEIARDVGLLALGTWAAWKPKSPFAIDNWL
                     FAPLTDPDAGASDAELDDKESAQ"
     assembly_gap    17418..17517
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <17518..17924
                     /locus_tag="PKJ61_06540"
     CDS             <17518..17924
                     /locus_tag="PKJ61_06540"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOC13517.1"
                     /translation="RNRNMTFLDNNLQNDSSTRSGIAAACSDVTGKYAAFHAEIYNHQ
                     PANEGDGYTDEVLRDTIPASVGITGEALTKFQACYDEQSTKAFVQGMNDLASKDGITG
                     TPSFHVNGKDIPLSDFSGVDPTTLGDLIKKNA"
     gene            18039..20621
                     /gene="valS"
                     /locus_tag="PKJ61_06545"
     CDS             18039..20621
                     /gene="valS"
                     /locus_tag="PKJ61_06545"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HOC13518.1"
                     /translation="MTVQPETSNVPATPSAGGTVPERPVLEGLEAKWSERWREQDTFA
                     FVRPESRAQVFSIDTPPPTVSGSLHVGHVFSYTHTDLIARYQRMRGKHVFYPMGWDDN
                     GLPTERRVQNFYGVRCDPSLPYVPDYTPPEKPDPKRQVPISRRNFVELCHQLTAIDEQ
                     AFENLWRTLGLSVDWKSLYATISDEAQTVAQRAFLRNLARGEAYLSEAPTLWDITFQT
                     AVAQAELEARDYPGHYYRVGFHAADGGRIHIETTRPELIASVCALIAHPDDARYQHLF
                     GTTVKSPVFGVEIPVLAHSAAEPDKGAGIAMCCTFGDLTDVQWWRELRLPTRTVIGRD
                     GRLHAETPEWLANPEPYAELAGKTTFSAREAMVAMLRASGDLDGEPKPTQRMANFYEK
                     GDKPLEIVSTRQWYIANGGRDDELKQELLARGEELAWVPDHMRHRYANWVNGLNGDWL
                     ISRQRFFGVPFPVWYALDADGEPDYAHPITAAEDALPVDPSSQCPPGYAEAQRGLPGG
                     FIGDPDVMDTWATSSLTPQLACGWERDEELWNLTFPMDMAPQAHDIIRTWLFSRVVRA
                     HHEHGVLPWRLAAISGFVVDPDRKKMSKSKGNVVVPTDILERFGSDAVRWRAAMARPG
                     MDSPFDETQMKVGRRLAMKILNASKFILGLPAAPADALVSEPVDLAMLATLAGVVKTA
                     TAGFEAYDYSAALEAAEKFFWQFCDDYLELVKERAYAAAPESASAQAALRQALNVLLR
                     LFAPFLPFVTEEVWSWWQSGSVHTSAWPDASELPSGGDAALLDDLASALIGVRGAKSH
                     AKVSMKTEAVRAEFSGTAEVLDRLRLAVTDLRAVGRLTGTVDWTLSDGPLSVAIELKQ
                     DAPA"
     gene            20618..21076
                     /locus_tag="PKJ61_06550"
     CDS             20618..21076
                     /locus_tag="PKJ61_06550"
                     /inference="COORDINATES: protein motif:HMM:NF025215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation family protein"
                     /protein_id="HOC13519.1"
                     /translation="MNRWLGVLLASAALLTGCAADGPRNSAGQVTAPVTVDSFSIQVG
                     DCVTKLESDSTTRLPLVPCDQEHAWEAFATAELTGDEFPGNNGVQNQGGQLCTEAFEP
                     FIGVAAKESKFEVRVLAPTKDSWAQQDREVVCLVGSSAGKLTGSLRGVAK"
     gene            complement(21266..22954)
                     /gene="ptsP"
                     /locus_tag="PKJ61_06555"
     CDS             complement(21266..22954)
                     /gene="ptsP"
                     /locus_tag="PKJ61_06555"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016461525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HOC13520.1"
                     /translation="MSDALHTTFHGIGVSAGTAVGPLVVVTPPPTAPADEAPTTDAPA
                     AVELVRGVLADVAAGLEARAATASDHAKPILEAGAMMARDPGLAMGVETQLQAGKGPT
                     NAITAAVEEYCVMFESLGGYMAERVTDLRDVRDRAVARLLGKPEPGVPTLSVPSILAA
                     HDLAPAETATLTAETCLGIVTEAGGPTSHTAILAAQLGIPAVVQAIGIMAVPAGVTVA
                     IDGGVGEITINPTDEETALLEERKKRRAAALAGGTGKGATKDGYPVALLANIGTADDA
                     RKAAEQPVEGVGLFRTEFLFLDRDTMPTVEEQTATYTEVFKAFGDRRVVVRTLDAGAD
                     KPLKFADLGPEENPALGRRGIRLGRIRTDVLDAQLEALAAAYKATGADVRVMAPMIAT
                     QAEAIWFAQRVRALGLPKVGVMIEVPAAAIRSRGILYNVDFASIGTNDLQQYTMAADR
                     MQGELAELLNPWEPALLDVIAVACDGGAAMGKPIGVCGEAGGDPALALVLAGLGVSSL
                     SMAPGKVPAVRTSLGLHTLTQCQQMAAAARAAGDATEAKQAVLEMADPVLLDLL"
     gene            complement(22959..23228)
                     /locus_tag="PKJ61_06560"
     CDS             complement(22959..23228)
                     /locus_tag="PKJ61_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009648125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HOC13521.1"
                     /translation="MATRQVSIASSVGLHARPASLFVQAVTASGLPVKIAKEGGSPVD
                     AKSILMVMALGAKCGETVTLTAEGDGADAVLDSLVELLSRDLDAE"
     gene            complement(23340..24632)
                     /gene="clpX"
                     /locus_tag="PKJ61_06565"
     CDS             complement(23340..24632)
                     /gene="clpX"
                     /locus_tag="PKJ61_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012919918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HOC13522.1"
                     /translation="MARIGETSDLLKCSFCGKSQKQVKKLIAGPGVYICDECIDLCNE
                     IIEEEFAETSDSGLIEELPKPREICSFLDSYVIGQETAKKALSVAVYNHYKRVQAQSA
                     GPGRRVADDELVELGKSNILLIGPTGCGKTYLAQTMARMLNVPFAMADATALTEAGYV
                     GEDVENILLKLIQAADYDVKKAETGIIYIDEIDKVARKSENPSITRDVSGEGVQQALL
                     KILEGTTASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFAGLEEIINARVGKRPLGFN
                     NDAAVRRPTSLNPFAEVRPEDLHKFGLIPEFIGRLPMISTVEQLDGTALERILIEPKN
                     ALIKQFRKLFELDGVDLEFTPDAIRAIAEMALRRGIGARGLRSILEEILLNVMYHVPS
                     DDDVAQVLVEREVITGGAAPQMIPRAKLARAERREKSA"
     gene            complement(24709..>25102)
                     /locus_tag="PKJ61_06570"
     CDS             complement(24709..>25102)
                     /locus_tag="PKJ61_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020391438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HOC13523.1"
                     /translation="SFTALTAIYDTMRYIKPDVQTICLGQAASAAAVLLAAGTKGRRL
                     ALPNSRILIHQPATEGGYGQSSDLEIQAREILRIRSLMESMLAESTGQDVAKISRDIE
                     RDKYLTAQEAVEYGIVDDILTSLKDAPR"
BASE COUNT         3909 a         8658 c         8344 g         3991 t
ORIGIN      
        1 agccgcgggc caacgccgag gcggtgctgg cccagtcccg cgaagccgcg gacgccggcg
       61 cctgcctggc cgtgttccct gagctcgcgc tcaccggcta ctcgatcgaa gacctgctga
      121 tgcaggacac cgtcctccag gcgaccaccg ccgccctcga ctggctggtg ccgcagactc
      181 aggacgtcgg cgcgatcatc gtggtcggcg ctcccctggt cagcctcaac cggatctaca
      241 actgcgcggt cgtgattcat cgcggggtcg tgcttggcgt ggtgccgaag tcctatttgc
      301 ccacctaccg ggagttctac gaacgccggc aagtcgcccc cggcgacgac gtcaccggcg
      361 agattcggat cgccggacag gacgtgcctt tcggctcgga tctgttgttc gccgccgaag
      421 atgtgcccgg cttcgtgctc aacgtggaga tctgcgagga catgtgggtg ccgattccgc
      481 cgtccgccga ggctgcgctg gccggtgcga ccctgatcgc gaacctctcc ggcagtccga
      541 tcactgtggc caaggccgag gagcgcaagc tgctctgcgc gtccggctcg gcgcggctgc
      601 tggccggcta cgtctactcg gcggccggtg agggcgaatc atccaccgac ctgtcctggg
      661 acggccagac gctgatctac gaggccggca ctctgctggg cgagtccgag cgcttccccg
      721 acggcccgcg gatgaccatc gccgacgtcg acctgggcag cctcgtccag catcggctgc
      781 gccaaggcac cttcgacgac aaccggcgca cccacgccga gcgcaccggc gagtatcgga
      841 cggtcacctt cgagctcgac cccccgtccg gcgatctcgg cctgcagcgg aagctggccc
      901 ggttcccgtt cgtgcccgcc gacccgaccc ggctcgcgct ggactgctac gaggcgtaca
      961 acatccaggt ctccggcttg gagcagcggt tgcgcgagat cggccagccc aagatcgtga
     1021 tcggcgtctc cggcggcctg gactccacgc acgcgctgat cgtggccgcg cgcgccatgg
     1081 atcgcatgga tcgcccgcgg acggacatcc tcgccttcac catgccgggc ttcgctacca
     1141 gcgcgcacac caagggcaac gccaccgcgc tcgccaagtc gcttggggtg accttcgccg
     1201 agctcgacat caccggcacc gcccggacca tgctcgacga gatgaatcat ccgttcgccg
     1261 aaggccagcc ggtgtacgac gtgacctttg agaacgtcca ggccggactg cgcaccgact
     1321 acctgttccg gctggcgaac cagcgcggcg gcattgtgct cggaacgggt gacctgtccg
     1381 agctggctct gggctggtgc acctacgggg tgggcgacca gatgtcgcac tacaacgtga
     1441 acggcggggt gccgaagacg ctgatgcagc acctgatccg gtgggtgatc agttcggcgc
     1501 agttcgacga caaggtctcg gctacgctgc aggcgattct ggacaccgag atctcccccg
     1561 aactcgtccc ggtgactgag ggcgaggtgc cgcagtcgac cgaggcgtcg atcgggccct
     1621 acagcttgca ggacttcaac ctttaccacg tgctgcgcct gggcatgaag ccgtcgaaga
     1681 tcgccttcct cgcctggcac gcctggagcg acgcgtccgc aggcgagtgg cccgaaggct
     1741 tcccgcacga cgagcggacg gcctacgacc tggccacgat caagaagtgg ctagaggtct
     1801 tctgcaagcg cttcttcggt ttcgcccagt tcaagcggtc agcgctgccg aacggcccga
     1861 aggtcgtgca gggcggctcg ctctccccac gcggtgactg gcgggcgccg tccgacacgt
     1921 ccgccgccct gtggctggag gatctggcca acatcccgtc ctgatgcacg cggggttggt
     1981 cgcgaggggt tggtcgcgac caacccctct gccgcctact cgactgcggg ggcgtccgcc
     2041 gtaaccactg gctcggcggc gggcgcatca acttcggccg gtgcaacctc gaccggaacg
     2101 ggttcgacgg gcggcccaac ctcgaccgga acgggatcga cgggcggccc aacctcgacc
     2161 ggaacgggtt cgacgggcgg cgcaacctcg acgggcggaa cctctgcggg caccggctcg
     2221 gccggcttgg gcttcggcgc gtccttcggg gtgcgagccg accgccgcga gccacccgaa
     2281 gcgctgctgc ccccacgcga ggtccggcga ccggactcac ggtcgccacc gtcggcgggc
     2341 gcctgcgacg ccaccggggc gtcgtgcacg tggtatccgc ggcccttgca ggtctcacag
     2401 gacacggtga aggcctcggc cagtcccgtc ccgatcttct tccgggtcat ctgcaccagg
     2461 ccgagagagg tcacctcggc cacctggtgc cgggtgcggt cgcgtcccag gcactccacc
     2521 aggcgacgga gcagcaactc acggttcgcc ggcagcacca tgtcgatgaa gtcgatcacg
     2581 atgatgccgc cgatgtcgcg cagccggagt tggcggacga tctcttcggc cgcctctagg
     2641 ttgttggagg tgaccgtggc ctcgaggttg ccggaggtgc cggtgaactt gccggtgttc
     2701 acgtcgatca cggtcatcgc ctcggtgcgg tcgatgatca acgagccacc ggacggcagg
     2761 aacaccttcc gctccagcgc cttggccacc tgctcgtgca ctcggaacgc gtcgaagatg
     2821 tcacccttcg aggtgcgatc ccacttctcc atgcgctcag ccaggtgcgg cgcgacgtgg
     2881 cgcacgtacg cgtccacggt gtcgtaggcg tcgtcagcct gaccattgcc gtcgatcacc
     2941 agcgagccga agtcctcggt gaaaaggtcg cgcacgatcc ggacggtcag gtcgggctcg
     3001 gcgtagagca gttggggcgc ctggccggcc ttgaccttct tctccagcac ctcccactgc
     3061 gccttcaacc ggttgacgtc gcgaaccagc tcatcttcgc tcgcgccttc ggccgccgtc
     3121 cgcacgatca cggacgcctg gggttcgatg agctcgtcga ggatgccctt gagccgcttg
     3181 cgctcggtct cgggcagctt gcgggagatg ccggacagat ggccgcccgg cgcgtagacg
     3241 atgtagcggc cgggaatcga gatgtgcgag gtcagccggg cgcccttggc cccgaccggg
     3301 tccttgctca cctgcaccag cacctgctgg cccgacttga agaccttctc aaccttcttg
     3361 tcctgaccgg tgacaccgaa cgagtcccag tcgatctcgc cggcgtagag cactgcgttg
     3421 cggccgcgac cgatgtcgac gaacgcagcc tccatcgaag gcagcacgtt ctgcacgcgg
     3481 ccgaggtaga tgttcccgat catcgatgtg gcggacgcgc ggtcgacgta gtgctcgacc
     3541 aggacgccgt cctcgatcac cgcgagctgc gagtagtcct cggcctgacg gatcagcatc
     3601 ttgcgatcca cggcctcccg gcgggccagg aactcggact cgctcaggat cggcgtccgg
     3661 cggcgcccag cggcgcggcc ctcacggcga cgctgcttct tggcctccat gcgagtcgag
     3721 ccttcgattc cggtgacctc gtcgctggcg gaactcgtgc gccggcgcgg ctcgcggacg
     3781 cgaaccacca cgtcgatcga ctcgtcctcc ggctcgccgg tgtcctcacc gcgacgacgg
     3841 cgacgccggc gccggcggga cgtgccttcg gctgtggctt cgtcggactc gccctcggta
     3901 tcgccgtcgt tctcgccgtc agccggctcc gcgaccgttg cgtcctcgtc gccggcgtcc
     3961 tcgctgtcga cggcggcttc gcccgcgcca cgacggcggc gacgcccgcc ccggcgacgg
     4021 cggcgacggc gcggctggcc ttcgtccccc tccatgcctt cggcgtcctc ctcggactcc
     4081 tcggcttcgt caagctcttc cgcctcgtcg agcggctctt cctcggcctc ctcttcgagg
     4141 tcggcggccg gttcgagcag cccgccgatg gctgcggcca aactgtccag cacctcgtcc
     4201 ggggtggctt cctcgcccag tccgaagctc tcggccaacg ccggacggcg ggtgcgacgg
     4261 cgtcccttgg ccaggtcggt caggctggcc gcgaccgcat cggcggcggc atcgttgtcg
     4321 gccggggcgt tcgcgtcggc ttcagccgga gtggcggacg ccgctaccgg agcggcagct
     4381 gtcgagtggc cggtttcggt cgccgcttcc gccacgacgg gtgcggcctt ggtcttcttg
     4441 gtcaccgcgc ggcgccggac gggcttcgcc ggcgcttcgg ctgccgacgg ctccgcggcc
     4501 ttggccggtt cggccggctc agtgggctca gccagcggga gcaccggctc agcggcagcg
     4561 ggcttggtac gggcgcgccg ggtgcgcttc tgcgggggct cggcggggac gtcggccgcg
     4621 gggctcgggg cgtcggcggc cggggtggtg gtctcggtgg attgcgcggt gccgctcaat
     4681 tcggcggcga ccgtcggctg tggcgcgctg gcggcctcgg cgggcgccgc aacgactgtc
     4741 tccaccggag cgggtggacc ggccggccga ctggcggcgc ggcgacgccg cggcggctga
     4801 ttgggctcgt ccggcgtggg cgaagcgctc tgcggatctt cggaatcgag catgatgctc
     4861 tccttcggcg gcgggggcgc cgcaaacgtg gctgccatgg gcagcgaagc ctgatcggtc
     4921 gaacgttcga cggtgcggcc cccgcggtcg gcttgcgtcg cggtgaggcc tcagcgtcgc
     4981 gatcggcctg tcacaccgat cgattcgaag gttgggggtc tggatgactc caacttgcca
     5041 gccagtatcc cacacctcag ccggctaccg cggagcgacc cgaaatcgcg tccgatcagt
     5101 ccgccaaggg atccaggatc cgctcgccgt cccaggtgcc ctgcgccaac cgggtgatca
     5161 gcgcgttgct gggaagctct ggcgcgagct gccgcagggc gttgagcacg tcgtcgggac
     5221 gcaccagcgg ctcaccgatc gccgagacca ggttcaggcc gttggctgag gcggtcagcg
     5281 agatcaccgc ggcgcgcaca tcgaaggtgc gcaggccggt cttggtcatc cgctgcacct
     5341 cgaacgagtc ggcagccagt agggcggcca cggcggtgct gagttcggct tccaccacgt
     5401 ccggcaggtc cacctgccag gcggacgtgg tcagccgctc ggcgagtgca ccggggctgg
     5461 ccaccaccac cttgacgatc accaggccgt ccggcatcgc agcgttcagt tgatcgcgaa
     5521 ccgcgtcggg ctcgcgcacc tcggccagcc ccaactcgag gtactcggcc tcagtggccg
     5581 ctccggtcgg ggacgcgttc gcgtagctga tccgcggatg cggcgagaag ccggacgaga
     5641 acgccatcgg gatctcggcg cggcggagcg cacgctcgaa cgcgcgtccg aagtcgcgat
     5701 gggacgcgaa gcgcgcccgg ccccgcttgg cgtactggac gcgcagcttc tgcaccggag
     5761 gcgcctgctg ctcgggctgt cgtctcgcgc tcacagccgg tcagcctacg gccttcacgg
     5821 ccgccggccc acccacgaag actaggctgc cgcgatgatc gagctgcgcc tggctgaacc
     5881 tgccgactac ccagcggtcg gagaactgac cgcgcaggcc tatctagccg acggcgccct
     5941 gcctgccgat gatccgtact acgaagtact tcgcgacgct gccgcccgtg ccgagtccgg
     6001 tgagctgtgg gttgcggtcg aagccgattc cctgctgggc accgtgacct ggtgtccgcc
     6061 cggatcgcgg ttccgtgaac tcgcccaccc gaatgagggt gagttccgca cgcttgcggt
     6121 ggcagttgct gcccgcggcc ggggtgcggg ccggctgctg gtggagaagt gcctgcagct
     6181 ggccaccgat gccggctcga ccgcggtggt gatctcgacg gcggagtgga tgaccaccgc
     6241 gcacgagctc taccggcggc tcggtttcgt cgaagcgccc gaacgggact ggtcggcacg
     6301 cccggatgtc cggctgcgcg ccttcgtccg tccgctcaac tgaccacccg agcccgtcga
     6361 gcctcagcgc agacctgcgt ccgactactc cgagacgcag aactcgttgc cctcggggtc
     6421 ggtgagcgtg taccagcggt gcggcccctg ggacgcctcg tggctgaagt tggcgccacg
     6481 cgcaaccaga gcgtcgcggg ccgcgtccag atcggcaccg acacccaggc gcacatcgag
     6541 atgcatccgg ttcttcacgg tcttcggttc gggcaccagt tggaacagca cccgccggca
     6601 cgggctgccc tcgggcgcgt ccgggacgcg aatcgcagca ccggtcgccc agaccaattg
     6661 atccttatag acgcgggtct cggcctcagt ggcgtaccca gcggtcacca tcttccggat
     6721 gaaagcctcg tcggtcggct ccacctccca gccaagggtc tcggcccacc aatcagcgag
     6781 cacatgcgga tcggcacagt cgagggtgac ctggaaggtg aggctcatcg gttctccttc
     6841 ggttgcggcg ggatgtcggg gacgccaatc gggcgcgtac ccagcacatc ttcatgccac
     6901 gcggcatacc cggcaacggc cgtcggcaga gtggcgaaga tgtgctcctc accgacagcc
     6961 tccacgaacc cggcggcagc gagttggtcg cgcacctcca tcttcacccg ggccatcgcg
     7021 aacacgatgc cgtccgcgtg cagggtccgg cgaagttcct ccagggtgtc gacagcggtt
     7081 aggtcgactt cgacattcgc ttcggcattg aggacgaacc acaatggcgg cggatccgcg
     7141 gcgcgaacgg cctccattgc ccgggtgagg aaatcgtcgg cattggcgaa gaacaacggc
     7201 gagtcgtagc ggtagaccac caggccgggc acttggctgg cctgtgggta atcctccaca
     7261 tcgtgcatgc cggcgacgcc cgggacgtag cccagcacgc cgtcgtgcgg gtgcgcgatc
     7321 cggcggatca ggtcgagcaa cgacagcccg attgcgatcc cgatgccgtt cagcagtccg
     7381 gtcgcaagga ccgcgacgaa agtgatcagt gacagcgcca gttcgctgcc gcggaacctc
     7441 gcgatccgac ccagctcggc caggtcgacc agacgcagcc cggcgtagat cacgaccccg
     7501 gccagcgcag cctgcgggaa ggccgccagc accggcgaga acaggtagat cgagcccacg
     7561 accatggcgg ccgccaccag cgagtggagt tgagtctggc tgccggcgga gtcgccgatc
     7621 accgtccggc tggccgacga cgacaccggg aaaccgccga ccagtccgtt ggcgaggttc
     7681 gccgcaccca ccgccaagag ctcggtgttg gcgtccacgc gctggccctt tcgggacgcg
     7741 aacgcgcggc cggtgaggat cacgtcggag tagcccacnn nnnnnnnnnn nnnnnnnnnn
     7801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7861 nnnnnnnnnn nnnnnnnngg ccctttcggg acgcgaacgc gcggccggtg aggatcacgt
     7921 cggagtagcc cacgacagcg atgccaacag cggccagtgt gagttgcagc ggatcgattc
     7981 cgttcaggtt cggccaggac accaccggta ggtcccgggg gatctcgccg atcacctcga
     8041 cgccgtaggc gcccaggcca ccgaaatgaa ccgctgcggc ggctagcagc atcaccagca
     8101 acggaccggg gagtttcggc gcccagcgat gcagggcgaa caggagcccg gccactacca
     8161 ggcccagcgt gagggtcggc aggtgcaccg aaccgagttg ggcgagcaac gaccccgttt
     8221 cggctacggg gttgtcgcca acgatcacga gcttggtcaa ggtggcgtac tggctgatca
     8281 ccatcagcaa cccgatgcca gccatataac cgaccagcac cggcttggag agcagattgg
     8341 ccaggaagcc gagtcgagcg agccagccca ctcccagcac gacaccgacc gcgatcgcga
     8401 ccagcgcagc cacctcggtg cgtcgctgca ggtccacctc gccgaccagt gcggcgatcg
     8461 ccgcagccgt catcagcgcg gtggtggact ccgggccaac ggacagctgc agagaggtgc
     8521 cgaggatggc gtagacgagc atcggtgcga gcactgccca cagcccggcg atcggcggca
     8581 tcccgatgat ctcggcgtag gccatcacct gcggcaccag gtaggccgaa acggtcacgc
     8641 cggcgagcag gtcgccgcgc agccagacgc ggtcgtagcc gagcagcaca cccagcccgg
     8701 gcgcccagcg acgccaccgg cgggcagccg ctgtgctcat ggcgacaacc tagcccacag
     8761 tgccgacaat cacttcggct tgatcgaggt gagccacggg ctggggacgg cgagcacctc
     8821 catccaggcc cggctggtct tgctcagagt gaccgtgcgt ccgttggtgg cggtggcggt
     8881 cagggtcttc atcagcccgc ccgagtaggt ctcggcgacc tcaatcttcg tgatgctctt
     8941 gaggttgaag acctttgcag cctgcttctg ggtgagcgtg cgtttccagg acttgtacgg
     9001 attgcggggc gccagcgccg agtacgggtc ggccacgccg ggcaggtagc tgagcggatc
     9061 ggccttgaac acgtcgctgt tcctggcggt gccggcacca gcggcgaaac cggtggacgc
     9121 gaagtacggc gtctcggcga atcctgcgga cgggtcgcgc agtatcagca cgcccgtctc
     9181 ggtgatggtc gacttcaccg cgtccaccca cggctggttg gcgctcgacc cggtcttctt
     9241 ccagccggtg tagttctggc tgcgttcgtc gtcgaagaga tggcagtcgc acgccggatc
     9301 accggtgtct acgccgagcg tttcccgcag cttgatcacg cgcttgatga catagttccg
     9361 ggccgcgatg gcctgggcct tcaatgcctc ggcgccgccg tagttgcccc aggaagccgg
     9421 catctcgtcc agtccgtaga gatacttggt gttcatggcc acctcgttga ccacgttcag
     9481 ccgcttgccg atggtggtca cctgcaggtt gccgtagtga tagctgccct gcgcgcccgt
     9541 cacggttgcg gtgccggtgg tggtcacggt cagccgggac gcggtcagcg acttcttacc
     9601 agccttcgct gtcaccccgc tgcccttgac cttcagggtg atcttgccgc tgctggtgcc
     9661 gagcaggacg cggccagcgg tatcgacgac agtgaagccg ccacccttga ccttgatcgt
     9721 ggccttcttg cgcttgtcga ccggcggccc gagcacctgc accttgaccg tccgggggtt
     9781 gttgttggtc cagccgatgg tggttcccgg gtagtagtac tggaggatct gctcggcgcc
     9841 gctccccgtc cgcgccagtt gataggcgcc gtactgcgac atcccgaccc cgtgcccggc
     9901 acccgaaccg gcgatcacga acttcgccgg cgccttcttg gccgcctcgg ccggtgccgc
     9961 cggcgccatg acgagagcca gcagtgccag cgccaccagg acggcaagac tacgcacgcg
    10021 atgcgttctg agcacgagtc gactgtagtc ggctagccac aggtctcgac aagctcgcca
    10081 ggccggactt cccctccagc tttgctcccg aatgggtctc tcgctcccga tagttcggga
    10141 gcaaaggtcc cattcgggag cgaagtcgcg atgggagagg gcgctcgacc cgccgccgcg
    10201 taggcagggg gtgggtcagc tggcttcggg acgggtgacc acggtcagcg ggagcagcga
    10261 acggccggta ggaccgatct ggatctcggt gccgaactgc ggacagacgc cgcagtcgta
    10321 gcagccggac cagcggcagt cgtccacctc cgcaccgtag agagcgtcct ggtagtcctg
    10381 ccagagccag tcgcggtcga ggccggcgtc caggtgatcc cagggcagca cctcaccctg
    10441 cgggcgctcg cgggtggtgt accaggccag atcgattccc aagggggtca gggcgcgctc
    10501 agcggcggcc gtccagcgct cgaaggagaa gaactcgctc cagccgtcga gcactccccc
    10561 gtcgcgccag accgcctcga tcaccgcgcc gacccggcgg tcgccacggc tcagcaggcc
    10621 ctcgatcatg ccgggcttgc cgtcgtggta gcgcatgccg atggcccgtc cgtagcgccg
    10681 gtcctcgcgg atcttgtcct tcagcagccg cagccggcgg tcgatcgtat ccggatcggc
    10741 ctgggccacc cactggaagg gagtgtgcgg tttgggtacg aacccgccga tgctgatggt
    10801 gcagcgcacg tctctggtgc cggcggcgcg gcgtccggct tcgatcacgt gggcggccat
    10861 gtcggcgatg ccgagcacgt cctcgtcggt ctcggtgggc aggccgcaca tgaagtagag
    10921 cttcacctgc cgccagccct ggctgaacgc cgcggcgacc gtgttgatca ggtcgtcctc
    10981 gctgaccatc ttgttgatca ccttgcgcat ccgctcggag ccgccctcgg gcgcgaaggt
    11041 cagaccggag cggcggccgt tgcggctcaa ctcattggcg aggtcgatgt tgaaggcgtc
    11101 cacccgggtg ctgggcaacg agagcgagac gaagctgccg tcgtagcggt ccgcgagttg
    11161 cttggtgacc ggcgcgatct cggagtggtc ggcgctggaa aggctcagga gcccgacctc
    11221 ctcgagcccg gtcgccgcca gcccgccctc aaccatcttg ccgatggtct cgatgttccg
    11281 ttcgcgcacc ggacgggtga tcatgccggc ctggcagaac cggcagccgc gggtgcaacc
    11341 gcggaagatc tccaccgagt accgctcgtg gacggtctcg gccagcggca ccacgggctt
    11401 ggcggggtag ggccactcgt ccagttccat cagggtgtgc ttggccacct tcgccggcac
    11461 ccccggacgg ttcggcacca ccgacgcgat cgtgccgtcc gggccgtagc tgacgtcgta
    11521 gagcgacggc acataggcca cgccgctggc ggccagccgg gccagcaggc cgctccggcc
    11581 gtccggacga ccggacgcct tccactcccg cacgatctcg gagatcgtca gcgcggcctg
    11641 ctcaccgtcg ccgagcacgg cggcgtcgat gaagtcggcg atcggttcgg gattgaacgc
    11701 ggcatgcccg ccggcgatca cgatcgggtc ctgctcacga cgatcggccg cgtgcagcgg
    11761 caaaccggcc aggtcgagtg cttcgagcag gttcgtgtag cccagctcgg tggagaagct
    11821 gacgccgaac aggtcgaacg ccgcgaccgg caggtggttc tccagggtga actgcggcag
    11881 gccgtgctcg cgcatcagct tggcgaggtc gggccagatc gagaacgtcc gctcggccag
    11941 cagccagtcg cgctcgttga ggatctcgta gaggatcgcc agcccctggt tcggctggcc
    12001 gacttcgtac gcgtccgggt acatcagcgc ccagcggacc tcgaccgaat cgaaggcctt
    12061 gctcaccgag ttcacttcgc cgccggtgta ctgaaccggc ttggtgaccc gcgcgagcag
    12121 cggttcgagc cgcgggtaga gcgattcgac cgaagggttc atcaaggctg tcatggtcgc
    12181 atcaggataa ccggccccac tgacatcgcc cgctgcgcgc ggcttgcgca gttcttcgcc
    12241 cgaacaaccc agcgatccgc cagttgcggc gttgcgcctc gcggcgggca cctcgacgat
    12301 agcgcgaaga cagtgcagta acggcggcga tgagggcgcg tcgaaccagc gctaccggtg
    12361 accgtccggc ttctgggacg gcggcgcgac ggccgtcgag cagttcgcgc gggcgtccct
    12421 gcacgctgcc gccacctgca agggcagccc gtacaccgcc atcgacgcca cgggcacctt
    12481 cagcggcgga ccttcgggaa ccttcgggct gaaacagggc cgctacacct acgccgggca
    12541 gcgcacagcg accggtgcga agatcaccgg cagtggtgtg accctcgact tcaccggctg
    12601 aagccacaga ttcggtggca actcagtcga tgtcggcgga atgtggcgcg gcgttctcca
    12661 cgacgaggtc ggcgcgcttg gccgggtcgc acgacgccag gtacagcagc tggccctggt
    12721 agtagcgcgc gttgcccggg tcctgcgggt cggcggacag cttgtggtcg cgctccgaca
    12781 tgcggtggaa cgactcgtcg aacggcacat ccagccacac cgacaggtcc cagatcttgt
    12841 tccgcttgct cgagcagagc tcgtcgcgat ggatgaacat gccatccacg atcactactg
    12901 cttcatcggg ggccacccgc cacggcgaat ggacttccga ttcggagtcc aggtcatacg
    12961 cggcggtgcg gtagcggccc gatccgtcgc ggccaagcgg ctccagcacc tcgtcggtga
    13021 agcgctcgta gtcgtagctg tcctcgaaat agcccttggc tgaggagcgt ccctgcgcgt
    13081 agcgcttcgt ctgcgggttc aggtagtggt ccatggaaat ccggatcact tcgatgccgc
    13141 tctcgcggag aaggtcggcc agctcgttgg caaaggtcgt cttgccagcg ccgtcgacgc
    13201 cgtcgatgcc caccagcgtc cgtccggacc gggcgcgaat gcgtgctcgc aaccacttca
    13261 ggccacccgg agcgtccagc ttctccaccg gctcagtacc catctcacac cctcagctct
    13321 gaaacctggg cgaccgatgt aacggtgcac ccgggcgaaa cccgagcttc caggatatgc
    13381 caagttggcg aaaaagcccg gatcgggggc tacggcccgg tcaccggctg tgcgccagga
    13441 gcggcggcgg actgcagccg ttgccgcttg gcgatccaca gcattgccgc catcaccgaa
    13501 aacgtgaaca ccagttgcac gagcacttct ggcccggcgt cgttggggcc acgggcgaag
    13561 acctcatcga gcggcacgaa cggcagggtt acctcgctga tcaggttgtt gaacacgtgc
    13621 agcgcgaccg cagcttctag cccgcccgtc cgccagacca gcaccgaaca gaccactccg
    13681 acgctgaagt agtagatgtt cagccagatg ttgtcggcgg cgtgcagaat catgaacacc
    13741 accgaggtga tcaccgtggc gaccacaagc cccacccgac gtgcgccgaa ccacgagccg
    13801 atgctgcgtg ccaggaaccc gcggacgccg tactcctccc cggctgcctg gaagggtgtg
    13861 gtgagcagaa tcgcagcgat caggaaggct gaatccgcgt tccagcgcat ttcggagccg
    13921 ccctcgcccc cgatcgcgaa actgaccgcc agccccacga ggaagatgcc ggccgcgacc
    13981 agcgagaacc gccagaacag cccccagcgg aaccctccga ggatggatga catccagcgt
    14041 ggccgctgcc cgaacaccgc ccaagcagtc aggccggcga tcgggatggc gaggccgaga
    14101 ccgatgttgt tcgcggtgaa cagcgccggg gtcatcggca cctcggtggt gagctcaccc
    14161 gggttggtcc ggccggtgaa cgcgtcgtag gcgagcgccg caacggtcac gacgagcgtg
    14221 ccgacgaacc acaccgccac gaacatcgcc agtcccaaca gcggacgcca ccagcggaaa
    14281 cggggtgccc ggaagaactc gtggtactcc ctgcgcacca ccggcagcgc tgcagttttc
    14341 accggcacgg ccggccagaa cacctgatct gggccaggtt ggaaaccggg cggatactgg
    14401 tgcgccggcg gcggatattg cgcagggtag gacggcggcg ggtaccacgc cccagtgggc
    14461 tcgggctgcg gtcgcggggg ttctgggacc ggccggtcgg tcagaccagg ttcgggaacc
    14521 acagtccgat ctcccgctcg gccgactccg gcgagtcgga cgcatggacc aggttctcgc
    14581 ggttcgacag ggagtagtcg ccacgaatcg tgcccggcgc ggcgacgacg gcgttggtcg
    14641 cgccgttcat cagccgaatc acctcgatcg cactcggccc ttccagcacc atcgcaaccg
    14701 tcggcccgct ggtgatgaac tcccgcagcg gcgggtacca gggctgcgcc acgtggtcgc
    14761 cgtagtgccg gtcggcaagg cccgcgtcca cctgtcgcag ttcgagcgcg acgatgctca
    14821 ggcccttcgc ctcgtaccgg gccaggatgg tgccggcgag cccacgccgc acggcgtcgg
    14881 gcttcagcag gacaaggctt cgttgcagat cggtcatggc cggaacccta ccgtccccgt
    14941 ccgacagtcg tggctgagcg cccgagccgg ccgcggcctg tggctggcgg gtcgaacagc
    15001 cgggatcagg cggacgctcg atgcgtcttc tcgatccgtc ggcccaacac gaagcagacc
    15061 gcccacagca gggcgaacat gccaccgacc gcgaacatca tctcggtgac gaagcccatc
    15121 cccaccgcga ccacctgggt cagccagccc agcggatagc ccagcggacg ccgcagcagc
    15181 gcggccgcca gcaccgccag cgcgacagcg cccagaccga tgccgaacgc gaccggcacg
    15241 ctgaccgcgt ccacctggac cattcccgcg atggcaaggc cgaacacgat ggcctcgaag
    15301 gccaggatgc tgagcatcac ccgacccatc gggttcgtcc gatcaagccc catcgggatt
    15361 gtcctcccgc accagcagcg ctcgggcctc gccggcggca tagaccgacc cggcgatcag
    15421 caccccggcg ccggcgccag cctcgtccgc cagccggacg gcctcctcga tcgccagcgc
    15481 catcgagggc gccacccgga cccgttcctc gccgaacacc tcggcggcca gaccgccgag
    15541 ggcgtccgca gagagcgcgc gggcggtgtt ggcgatcgtc gtggtcacca cggtggtcat
    15601 cgcttcggcg aacaccgcca acacgccgct gacatccttg tcggccatca tcgccaccac
    15661 gccgatcagg ggcgcgaagt cgaatgcctc ggtgatgccg tcgatcgtcg cccgagcccc
    15721 atgcgagttg tggcaggtgt ccagcacgac cgtcggcgag cggcgaacga tttcaagccg
    15781 ggccggcgcg gtcacctgtt cgagaccctc ggcgatcacg tcggctggca gcgggctgcc
    15841 gacgaacgcc tccactgcgg ccactgccag ggcggcgttg cgagccatgt gtgcgccgaa
    15901 cagcggcagc accagatcgc ccagcggccc ctcggacgtt tccagacgca acacctgccc
    15961 accgatggcg ggcacccggt cgatgagacc gaactccatg ccctcccgcc ggacctcggc
    16021 gcccacgtcc gcgcagcgag ccagcaggac gctcgcagct tcgggctgct gggcggcgag
    16081 cacggctcgg ctgccggcct tgatgatccc ggccttctcg gccgcgatct ctgccagggt
    16141 cgacccgagg atcgcggtgt ggtcgaaatc gatcggcgcc acgacggcga ctgcggcgtc
    16201 ggtgacgttg gtcgcgtccc attcaccgcc catgccgacc tcgacgacgg cgacgttcac
    16261 cggagcgtcc gcgaaagcgg cgtaggcgag tgcggtcatc acctcgaaga aagtcattgc
    16321 gatgccgtcg atcgcctgtt cgtccaccag cttcaccagc ggcgccacgt tctcgacgag
    16381 ttcgtcgaag acctcgtcgg cgattggctc gccatcgatg gtgatgcgct cggtgaggtt
    16441 gaccaggtgg gggctggaga agcgtccgac ccgaaggccg agcgcgcgca gcagcgagtc
    16501 gatcatcgcc gcggtgctgc ccttgccgtt ggtgccggtg atcaggatca ccgggcaggc
    16561 atcctgcggc gatccgagca ggtcacaaag ggcacggatc cgggcctggc tgcggccgat
    16621 cttatgctcc ggccagcgcg actggagctc ggcgacgatc tcagcgtggg ttcggctcat
    16681 ggtgcgctca gcctagatga tcgctgcgcc cgatcaacgg gaccgcctgc gtataatcca
    16741 tcgggtgacc gaaacgcccg ccgtcggagc gctccgacga ccgtggacgg agtgggtcac
    16801 cttggcctgc cggctcggtc ttggtggcgt gctgctggtg gctgggctgt tgaaggtggg
    16861 gcacctggac gcgtccgtgc agtcggtgcg gctgtaccaa ttgctgccct gggaaatcac
    16921 cgcgttcgtc ggttcggcgt tgccgatcat cgagatcgcg atgggcctgc tgctgatcac
    16981 cggcaccttc acccgcatct ccgcgattct gggcggcctg ctcatgctgg cattcatcat
    17041 cggcatcgcc tcggtgtggg cgcgcggcat ctccatcgat tgtggctgct tcgccgccgg
    17101 cggcgaggtt gacccgagcg cgacccaata tccactggag atcgcccgcg acgtcggcct
    17161 gctcgcgctc ggcacgtggg ccgcttggaa gcccaagtca ccgtttgcga tcgacaactg
    17221 gctgttcgcc ccgctgactg acccggacgc cggtgcgtcc gatgccgaac tcgacgacaa
    17281 ggagtccgcc caatgaccga gaacaaccgc gaacgcctgc ggaagagttc ggaagccaag
    17341 gccaagcagc agcggctgaa tcgcatcatc ggcgttggtg cggccatcct cgcggtcgtg
    17401 ctgatcgggg tcttcgcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntgc
    17521 gcaaccgcaa tatgacgttc ctggacaaca atctgcagaa cgactcgtcc acccgctcgg
    17581 gcatcgccgc ggcatgctcg gacgtcaccg gcaagtacgc tgccttccac gccgagatct
    17641 acaaccacca gccggcgaac gagggcgatg gctacaccga cgaggtgctg cgcgacacca
    17701 ttccggcctc ggtagggatc accggggagg cgctgaccaa gttccaggca tgctacgacg
    17761 agcagtccac gaaggccttc gtgcagggca tgaacgacct ggcgtccaag gacggcatca
    17821 ccggcacgcc cagcttccac gtgaacggca aggacattcc cctgtccgac ttctccggtg
    17881 tcgacccgac cacgctgggc gacctgatca agaagaacgc ctgaccctgc gagcctgatc
    17941 gccgcggtag ctggtcgggc tcgtcaagtc accacgcaac aatctcgccc atcttggcga
    18001 ttgggtgagt gtctgacccg ctcaataaga tgctctccat gactgtgcag cccgagacca
    18061 gcaacgtgcc cgccactccc tcggccggcg gcacggtacc ggagcgtccg gtgctggagg
    18121 gcctggaagc caagtggtcc gagcgctggc gcgagcagga caccttcgcg ttcgtgcggc
    18181 ccgagtcgcg cgcgcaggtc ttcagcatcg acaccccgcc gccgaccgtc tccggctcgc
    18241 tgcacgtggg ccacgtgttc agctacaccc ataccgatct gatcgcgcgc taccagcgga
    18301 tgcgcggcaa gcacgtcttc tacccgatgg gttgggacga caacggcctg cccaccgagc
    18361 gtcgggtgca gaacttctac ggggtgcgct gcgacccgtc gctgccgtac gtgcccgact
    18421 acaccccgcc ggagaagccg gacccgaagc ggcaggtgcc gatcagccgg cggaacttcg
    18481 tcgagctctg ccaccagctc accgcgatcg acgagcaggc tttcgagaac ctgtggcgga
    18541 cgctgggcct cagcgtcgat tggaagtcgc tctacgccac catctccgat gaggcgcaga
    18601 cggtcgcgca gcgcgccttc ctgcgcaacc tcgcccgcgg cgaggcgtac ctttccgagg
    18661 ccccgacgct gtgggacatc accttccaga ccgcggtcgc gcaggccgaa ctagaggccc
    18721 gcgactaccc gggccactat taccgggtcg gattccacgc cgcggacggc ggacgcatcc
    18781 acattgagac cacccgtccc gagctgatcg cgtccgtctg cgccctgatc gcacacccgg
    18841 acgacgcccg ctaccagcac ctcttcggca ccacggtgaa gtcgccggtg ttcggtgtgg
    18901 agatcccggt gctggcgcac tcggcggctg agcccgacaa gggtgccggc atcgcgatgt
    18961 gctgcacctt cggcgacctc accgacgtgc agtggtggcg tgagctccgc ctgcccacgc
    19021 gcacggtcat cggccgggac ggacgcctgc acgccgagac tccggagtgg ctggcgaacc
    19081 ccgagcccta cgccgagctg gccggcaaga cgaccttcag cgcgcgcgag gcgatggttg
    19141 cgatgctgcg cgcgtccggc gatctggacg gcgagccgaa gcccacccag cgcatggcca
    19201 acttctacga gaagggcgac aagccgctgg agatcgtgtc cacccggcag tggtacatcg
    19261 ccaacggcgg ccgcgacgac gagctgaagc aggagctgct ggcccgcggc gaggaactcg
    19321 cctgggtgcc cgaccacatg cggcaccgct acgccaactg ggtcaacggc ctcaacggcg
    19381 actggctgat cagccggcag cgcttcttcg gggtgccctt cccggtctgg tacgccctcg
    19441 acgcggacgg cgagcccgac tatgcgcacc cgatcactgc ggccgaggac gcgctgccgg
    19501 tcgacccgtc cagccagtgc ccgcccggct acgccgaagc ccagcgcggc ctgcccggcg
    19561 gtttcatcgg cgacccggac gtgatggaca cctgggccac ctcgtcgctc accccgcagc
    19621 tggcctgcgg ctgggagcgg gacgaggagc tgtggaacct gacgttcccg atggacatgg
    19681 ccccgcaggc gcacgacatc attcgcacct ggctgttctc tcgggtggtg cgcgcgcatc
    19741 acgagcacgg cgtgctgccc tggcggctgg ccgcgatctc cggcttcgtg gtcgacccgg
    19801 atcgcaagaa gatgagcaag tcgaagggca atgtggtggt gcccaccgac atcctggagc
    19861 gtttcggttc ggacgcggtg cgctggcgcg ctgccatggc ccggccgggc atggattcgc
    19921 cgttcgacga gacccagatg aaggtaggcc gtcgcctggc gatgaagatc ctgaacgcgt
    19981 ccaagttcat cctgggcctg ccagctgccc cggctgatgc gttggtgagc gagccggtcg
    20041 acctggccat gctggccacg ctggccgggg tggtgaagac cgccaccgcc ggcttcgagg
    20101 cctacgacta ctccgccgcg ctggaggctg ccgagaagtt cttctggcag ttctgcgatg
    20161 actacctgga gctggttaag gagcgcgcct atgcggcggc accggagagt gcctccgccc
    20221 aggccgcgct ccggcaggcg ctcaacgtgc tgctgcggct gttcgctccg ttcctgccgt
    20281 tcgtcaccga agaggtctgg tcgtggtggc agtcgggttc ggtgcacacc tcggcgtggc
    20341 cggatgcgtc cgagcttccc agcggtggag acgccgcgct gttggacgac ctcgcgtccg
    20401 cgctgatcgg cgtccgcggg gccaagtcgc acgccaaggt ctccatgaag accgaagcgg
    20461 tgcgtgctga gttcagcggc actgcggaag tgctggatcg gctgcgcctg gccgtcaccg
    20521 acctgagggc ggtcggccgg ctcaccggga cggtcgattg gacgctcagc gatggcccgc
    20581 tgagcgtggc cattgaactc aagcaggacg cccctgcatg aaccgctggc taggcgtcct
    20641 gctggcctcc gccgcgctgc ttaccggctg cgcggcggac ggaccgcgga acagcgccgg
    20701 ccaggtgacc gcaccggtga ctgtcgactc gttctcgatc caggtcggcg actgcgtgac
    20761 caagttggag agcgactcga cgacccggct gccgctggtg ccgtgcgacc aggagcatgc
    20821 gtgggaggcc ttcgccaccg ccgagctgac cggtgacgag ttcccgggca acaacggtgt
    20881 tcagaatcag ggcggccagc tctgcaccga ggcgttcgag ccgttcatcg gcgtggccgc
    20941 gaaggaatcc aagttcgagg tgcgcgtgct cgccccgacc aaggactcct gggcgcagca
    21001 ggaccgcgag gtcgtctgcc tggtcggatc gagcgcaggc aagctcaccg gatcgctgcg
    21061 cggcgtcgcc aagtagcgga acggcccacg agcttcccca gcacggcgca accgccctcc
    21121 cggagtcacc gtcatcccgg cgcaacggcc ctcccggctc acgtcatccc ggcgcaggcc
    21181 gggatctcag cgtgatcggc ggaacagttg aagccccttg gcacaccgcc ggtcgagggc
    21241 ccttcgtcaa gctcagggac cgtagtcaga gcaggtcgag cagcaccggg tcggccatct
    21301 ccagcacggc ctgcttggcc tcggtcgcat ctccggcggc acgggccgcc gccgccatct
    21361 gctggcactg ggtgagcgtg tgcaggccga gcgaagtccg caccgccggc accttgcccg
    21421 gagccatcga caggctggag acgccgagtc cggccagcac cagcgcgagc gccggatcac
    21481 cgccggcctc accgcagacc ccgatcggct tgcccatcgc cgcgccgccg tcgcaggcga
    21541 ccgcgatcac gtccagcaac gccggctccc acgggttcag cagctcggcc aactcgccct
    21601 gcatccggtc ggcagccatc gtgtactgct gcagatcgtt ggtgccgatg gacgcgaagt
    21661 cgacgttgta caggatgccc cggctgcgga tcgccgccgc cggcacctcg atcatcaccc
    21721 cgaccttcgg cagccccaat gcgcggacgc gctgggcgaa ccagatggcc tcggcctgcg
    21781 tcgcgatcat cggcgccatc acccggacgt ccgcgccggt ggccttgtac gcagccgcca
    21841 gcgcctccag ctgagcgtcg agcacgtccg tgcgaatgcg tcccagccga atcccgcggc
    21901 ggccgagggc cgggttctcc tccgggccga gatcggcgaa cttgagcggc ttgtccgccc
    21961 cagcgtccag ggtgcgcacg actactcgcc gatcaccgaa ggccttgaac acttcggtat
    22021 aggtggctgt ctgctcttcc acagtcggca tggtgtcgcg gtcgaggaag aggaactccg
    22081 tccggaagag gccgactccc tccaccggct gctcagcggc cttgcgggca tcgtccgcgg
    22141 tgccgatgtt ggctagcagt gctaccgggt aaccgtcctt cgtcgcccct ttgccggtac
    22201 cgccggcgag cgccgccgcg cgccgcttct tgcgctcctc cagcagcgcg gtctcctcgt
    22261 cggtcgggtt gatcgtgatc tcccccaccc cgccgtcgat ggccacggtc acgccagccg
    22321 gaaccgccat gatgccgatc gcctgcacca ccgccggaat gcccagctgg gcggcgagga
    22381 tcgcggtgtg gctggtcggt ccacccgctt cggtgacgat gcccagacag gtttccgccg
    22441 tgagcgtggc ggtctccgca ggcgccagat cgtgggcggc gagaatgctc gggacgctca
    22501 gcgtcggcac tccgggctcg ggcttgccaa gcagcctcgc gaccgcccga tcgcggacgt
    22561 cccgcagatc ggtgacccgc tcggccatgt agccgccgag cgactcgaac atcacgcagt
    22621 actcctcgac cgcagccgtg atcgcgttcg tcgggccctt gccggcctgc aactgggtct
    22681 cgacgcccat cgccagcccc ggatcccgcg ccatcatcgc gccggcctca aggatcggct
    22741 tggcatgatc gctcgcggtg gccgcgcgcg cttcaaggcc ggcggcgacg tccgccagga
    22801 cgccccggac caactcgacg gcagcaggtg catccgtggt gggggcctca tcggcaggtg
    22861 ccgtcggcgg cggggtcacc accaccagcg ggccaaccgc ggttcccgcg ctgacgccaa
    22921 tgccgtggaa ggtggtgtgc agggcgtcgc tcatcggcct actccgcgtc gaggtcgcgg
    22981 gacagcagct cgaccaggga gtccaggact gcgtccgctc cgtcgccctc agcggtgagc
    23041 gtgacggttt cgccgcactt ggcgccgagc gccatcacca tcaagatgct cttcgcgtcg
    23101 accggcgagc cgccctcctt cgcgatcttc actggcaacc cggacgcggt gaccgcttgc
    23161 acgaacaggg acgccggacg ggcgtgcagg ccaacggacg aggcgatgct gacttggcgg
    23221 gtggccattg cggtctccta agcgagtagc gggaatcgac tggtccacaa tggcacaacc
    23281 cagccgtccg ggagagccct tgaccgttgt tcgctcggac attccccaag tgggatccct
    23341 caggcgctct tttcgcgccg ctcggcgcgg gccagcttgg cgcgcgggat catctgcgga
    23401 gccgcgccac cggtgatcac ctcgcgctcc accagcacct gcgccacgtc gtcgtcgctg
    23461 ggcacgtggt acatcacgtt gagcaggatc tcctcgagga tcgaacgcag cccgcgggcg
    23521 ccgatgccgc ggcgcagggc catctcggcg atggcgcgga tcgcgtccgg ggtgaactcc
    23581 aggtcgaccc cgtccagctc gaagagcttg cggaactgct tgatcagggc gttcttcggc
    23641 tcgatcagga tccgctcgag cgcggttccg tccaactgct cgacggtgga aatcatcggg
    23701 aggcgtccga tgaactcggg aatcagcccg aatttgtgca ggtcctcagg gcgaacctcg
    23761 gcgaacgggt tcaatgaggt cggccggcgc accgcggcgt cattgttgaa gccgagcgga
    23821 cgcttcccga cccgcgcgtt gatgatctcc tccaggccgg cgaacgcgcc gccgactatg
    23881 aacaggatat tcgtggtgtc gatctggatg aactcctggt gcgggtgctt tcggccaccc
    23941 tgcggcggca ccgaagcagt ggtgccctcc aggatcttca gcagcgcctg ctgcacgccc
    24001 tcgccggaga catcacgggt gatcgacggg ttctcgctct tgcgcgccac cttgtcgatc
    24061 tcgtcgatgt agatgatgcc ggtctcggcc ttcttcacgt cgtagtcggc ggcctggatc
    24121 agcttcagca ggatgttctc gacatcctca ccgacgtagc cggcctcggt gagcgcggtc
    24181 gcgtccgcca tcgcgaacgg gacgttcagc attcgcgcca tggtctgcgc gaggtaggtc
    24241 ttgccgcagc cggtggggcc gatcaacagg atgttcgact tgccgagttc gacgagttcg
    24301 tcatcggcga cccgacgacc cgggcccgcg ctctgggcct ggacccgctt gtagtggttg
    24361 tagaccgcga ctgacaaggc tttctttgcg gtctcctgcc cgatcacata ggagtcgaga
    24421 aaggagcaga tctcgcgtgg cttgggcagt tcctcgatca gtccggagtc gctggtctcg
    24481 gcgaactcct cctcgatgat ctcgttgcac aggtcgatgc actcgtcgca gatgtacaca
    24541 cccggtcctg cgatgagctt cttgacctgc ttctggctct ttccgcagaa cgaacacttc
    24601 agcaggtccg acgtctcgcc gattcgtgcc acgtcgcgcc ttccgttgag tggttgcgcc
    24661 gaccgtaacc cggctcctcc ccttccgagg ggaagagccg gacgctggtc agcggggagc
    24721 gtccttcaaa gaggtcagga tgtcgtcgac gatgccgtac tcgaccgcct cctgggcggt
    24781 gaggtacttg tcgcgctcaa tgtcgcggct gatcttcgcc acgtcctggc cggtgctctc
    24841 ggcgagcatc gactccatca gcgaacggat gcgcagaatc tccctggcct ggatctccag
    24901 gtcggaggac tgaccgtagc caccttcggt ggccggctgg tggatcagga tgcggctgtt
    24961 gggcagcgcc agccgccgtc ccttggtgcc ggcggccagc agcacggctg cggcggacgc
    25021 ggcctggccc aggcagatgg tctgcacgtc gggcttgatg tagcgcatcg tgtcgtagat
    25081 cgcggtcagc gccgtgaacg ag
//
LOCUS       DAOPRQ010000038        24216 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_1714_length_24216_cov_13.323662, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000038 DAOPRQ010000000
VERSION     DAOPRQ010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 24216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24216
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1714_length_24216_cov_13.323662"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="PKJ61_06575"
     CDS             <1..272
                     /locus_tag="PKJ61_06575"
                     /inference="COORDINATES: protein motif:HMM:NF016141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HOC13524.1"
                     /translation="KLSPMLIIGLVLFGMLVGAAAQLILGSSRKGIDWALAFVSGLVG
                     SFVGGLLISLLSGDGLELRPSGLIGSVVGAVIVTAIWQWRQRQSR"
     gene            312..1028
                     /locus_tag="PKJ61_06580"
     CDS             312..1028
                     /locus_tag="PKJ61_06580"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOC13525.1"
                     /translation="MSQPVTRAPLVLIVEDEVPLRESVARHLAASGFTAVEAGSVAAG
                     LAAAWEHQPDVVLLDVLLPDGSGYDLCAKLRPITAAPIIYLTALGQDRNVVQGLATGA
                     DDYIPKPFSLEVLTARIQAQLRRTSAPRKGRIDLPPLHLDYLTGEVLLDGREINLTKM
                     ELQLLGFFATNVGVGATQAQLLTSVWRSKADIPTNTVRQTVSSLRRKLALDAGSAFEL
                     EFSADKRYVFRQVRFDPSPT"
     gene            complement(1138..2475)
                     /locus_tag="PKJ61_06585"
     CDS             complement(1138..2475)
                     /locus_tag="PKJ61_06585"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOC13526.1"
                     /translation="MTETPAWRWLRRLPGSLPPRAGEDFREQLIAVTAMVGVPISWAS
                     ALINVVLGSPVWTVLLNFFAGLAILGLVWFGRVSGRYRLTYAIAVGALFVVLFPGLFF
                     AGGGWEGGMPIWFAFALAFSAIILEGPALLVMVPLEAVVFATSLVLAFSYPQLVVPLP
                     SAFAKFVDIIYSMLATGLALAIALRLLIRLYQRNKDQLTARNAELARLDATRAEFLAM
                     VAHELNTPLAVIRTHTDEAGLALATTDANARADAGTSRVVADLGVIGSETDRLAHLVT
                     QLLDLGRISERRLDVDLRPLALDALVQQTLQAYRPIFAQHGNTLVVPPGGATPTVLAD
                     RDRVIQVLVNLLSNASRHTHNGTISVRVGEAAGFAELIVADSGEGIAPDLLDQLGRQP
                     VRTHQSGVRSARDTGLGVGLLICREIAVAHGGDLHVASTPGHGTTVRLTLPLT"
     gene            2754..3596
                     /locus_tag="PKJ61_06590"
     CDS             2754..3596
                     /locus_tag="PKJ61_06590"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOC13527.1"
                     /translation="MSVPAEILPINVRPEPFSVYTTPEFWNDPHLSAQLLQAHRDPDS
                     GPASRPHAFIERSVEWLLQALGLRPGSRVLDLGCGPGLYANRLAGHGIQVTGIDVSRS
                     SLTYARTGAQQSGPAAEFIEGNYLEADLGSGYDAALLIYADVCALSFEQRRSFLQRVL
                     SALLPGGLFAFDVTAAPRFTHYHEGIELLPADGFWAAGPHVATRATWTYPELRLVLER
                     YVIQTPTKVREFWNWTHCLTAFEVEAELASAGFADFRRFGDVAGADYDPGADTFALIA
                     RRPG"
     gene            complement(3613..4437)
                     /locus_tag="PKJ61_06595"
     CDS             complement(3613..4437)
                     /locus_tag="PKJ61_06595"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOC13528.1"
                     /translation="MTSDPEPGERVAAGRQVPSGNQLVLIAIGIGLASGFFSGLFGVG
                     GGLIVVPALAVLLGLDHRRAVGTSLLAIVPAVTASLVSYAATGSVNLAVGGLLAVGAI
                     VGAQAGVWLLGRISRRAAQWIFVGFVVVMIVQLLSLVPERGADLPIDVWRGIGLVVLG
                     LGAGVLSALLGVGGGGVVVPVLMVWFGIGDLSAKGASLVMMLPGVASGLVANLRKQFV
                     DVRLGLMIGAAAVVSSPLGVWVAHAIPPQPAAWLFAGFLLFVGGTMAREALRPPAA"
     gene            complement(4434..5861)
                     /gene="purB"
                     /locus_tag="PKJ61_06600"
     CDS             complement(4434..5861)
                     /gene="purB"
                     /locus_tag="PKJ61_06600"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020579029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HOC13529.1"
                     /translation="MIPNVLGTRYASTAMQEIWSPEAKIIAERKLWVAVLRAQAELGV
                     DFGGDDPDAVIAAYERVLDQVDLASINARERVTRHDVKARIEEFNALAGHEHVHKGMT
                     SRDLTENIEQRQLLSSLELIRERIVTVLVNLARLAVQYRDLPMAGRSHNVAAQTTTLG
                     KRFASAADELLVAFDRVDELIGRYPARGIKGPMGTGQDMLDLLDGDAVRLAELERRVA
                     EHLGFARVFTATGQVYPRSLDYDVLTALVQVAAGPSSLATTIRLMAGQELVTEGFAEG
                     QVGSSAMPHKMNTRSCERVNGLAVVLRGYASMAGELAGNQWNEGDVSCSVVRRVALPD
                     ACYALDGMFETFLTVLVEFGAFPAVVEAELQRFLPFLTTTKILMAAVRAGVGRETAHE
                     VIKEHAVAVALAMRAGAATNDLYDRLAADARLGLTREQLDAVVVDPLELTGSARDQVD
                     TIAARVGELAVRFPEGAAYRPGAVL"
     gene            complement(5858..7159)
                     /gene="purD"
                     /locus_tag="PKJ61_06605"
     CDS             complement(5858..7159)
                     /gene="purD"
                     /locus_tag="PKJ61_06605"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015071397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HOC13530.1"
                     /translation="MTQTVLVIGSGGREHALAWSLSRDPSVGAVHVAPGNPGTALVGT
                     NHPVDALDGDAVAALAVELGATLVVVGPEAPLVVGVADAVRARGIAVFGPSAEAARLE
                     GSKAFAKEIMEAAGVPTADARLCLDMGEVIDALDEFGPPYVVKDDGLAAGKGVLVTDD
                     RDAAIRHAASCRRVIIEEYLDGPEVSLFALSDGTNVVPFEPAQDFKRVGDGDAGPNTG
                     GMGAYTPLPWAPSGLVDEVVRTVIQPTVDELRRRGTPFIGLVYAGLALTSRGLRVVEF
                     NARFGDPETQPLLTRLKSPLGELLYAAATGELAGRSLEWGSGAAVGVVIAAAGYPDAP
                     RKGDPIAGAELGGVFHAGTAQDPSGQLVSAGGRVLTVVGTGDTLAEAREAAYAKVAQI
                     TLPGGFHRTDIAAAAADAERKGDSPFSVQDAGSSTGSGSDA"
     gene            7362..8471
                     /locus_tag="PKJ61_06610"
     CDS             7362..8471
                     /locus_tag="PKJ61_06610"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HOC13531.1"
                     /translation="MSIDTGTPTWLRTYNDRAAFRLLLEHGPLSRSMLGKLSGMSKPT
                     AGQMITRLERLGLISPVGEVTGARGPKATAYGARADSMTGVAVSILADRIEAVLVDPT
                     DAGHPIADLSIEGVERSPVTDVTRAVDAACATAGVDRASVSVVAVGVQAAVDADTDRL
                     TFTDTLPGWPERGARALIEAGTGLTTTVENDVNLAAMAERAITDEADFVFLWLGSGLG
                     TGINVDGVVRRGASGSAGEIGYLEVPRSAIALDPAAETFTDLLGGPAIARLLGHPAAD
                     FTAALKELPQNEPVLAELAVRVVMLVDVITAVLDPACVVLGGPTGLAGGDRLAELVQA
                     RVRASRAGDEPVLQGARQLLVANIRERLEAAIAAS"
     gene            8565..9821
                     /locus_tag="PKJ61_06615"
     CDS             8565..9821
                     /locus_tag="PKJ61_06615"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012918855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HOC13532.1"
                     /translation="MKLAIVGGGSTYTPELIDGFARYADVLPLSEIYLVDPDAHRLEL
                     VTGMAQRMLARAGNPAKVVGTANLAEGVEGADAVLVQLRIGGQAARKADETWPHEVHC
                     IGQETTGPGGFAKALRTVPVMLQIAEVVAERAKPDAWLIDFTNPVGIVTRALLQAGHR
                     AVGLCNVAIGFQRRFAAVLGVQHDQVSLGHVGLNHLTWERSVTVDGVDRLPEMLGGGL
                     ADIAEEVELPPALLAQLGVVPSYYLRYYYAHDEVLAEQLAAPTRAEAVQAIEDELLKL
                     YADPSVDTKPESLEKRGGAFYSEAAVELLAAMRGAAGPSRVVNVRNDGLLPFLPADHV
                     VEVPAQFRDGRFVADPVAPLPDDVAGLIAAVAGYERLALDAAVHGGRDRVLRAMRAHP
                     LVLQHDRAERLTDLLMAENARYLPWA"
     gene            9818..10771
                     /locus_tag="PKJ61_06620"
     CDS             9818..10771
                     /locus_tag="PKJ61_06620"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HOC13533.1"
                     /translation="MNEVVVAVDGGGTKADAVAFDLTGRLVASSRGPGGNPQSDGVAA
                     SLAMVDGLIREVAGSARVLRAGLYLSGLDLPQEVTAYREAAANSPWAASGLDVDNDLF
                     ALLRAGTEEPDAVAVVCGTGINAVGIRADGATARFLALGPISGDWGGGLGLGQEALWY
                     AARDVDGRGPHTALTTAISTHFGVDVSGLITQFHLGERSRSELAELAPAVFAVAVAGD
                     EVAGSLVDRQAKEIVDLARAALTRLELTHSAVPIVLGGGILRAGDARLLDGIRTGLAE
                     VAPTVRLVHVTKPPILGAALLTLAHAGADTETLARAQTALL"
     gene            11139..11933
                     /locus_tag="PKJ61_06625"
     CDS             11139..11933
                     /locus_tag="PKJ61_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HOC13534.1"
                     /translation="MAANVDAEDTRLLPHERHLRILEQARSRSRVEVALLADELEVTS
                     ETIRRDLTMLERQGLLRRVHGGAVRIERLGYEPSVNARREHRVAEKSRIGRAARDLLP
                     DGGSILVDSGTTTLELVRNLPSDLPLTVVTNSLQAAVVLGDYPRVELLLLGGHVRELT
                     GAAVGTWTTGALAHLRVDVAFMGTNGVTPELGLTTPDQAEAEVKRAMVKAANRVVCLA
                     DHTKVGLTQLCRFAGLDEVDVIVTDRGLEAGLAEELTTAGPEVIRA"
     gene            11930..12877
                     /gene="pfkB"
                     /locus_tag="PKJ61_06630"
     CDS             11930..12877
                     /gene="pfkB"
                     /locus_tag="PKJ61_06630"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HOC13535.1"
                     /translation="MIVTLTPNPSVDRTISVATLERGAVMRATASREDPGGKGLNVSR
                     ALALNGTVTAAVLPVGGAYGRMLLALLESGDVDVRPVPIGAAIRANVAIVEPDGTTTK
                     LNEQGPQLHPAELAALRQAVLDAAAGASWVVGCGSLPPGVDDSFYAELVGLCHANGVR
                     VAVDSSGPSMAAALAAAPDLIKPNRIELAEAVGRDLDTVGAVVDASRELVASGVGTVV
                     VSLGRDGALLIDAERVVHAVASINHPLSTVGAGDSLLAGYLHAVDAGADPVAALRTAV
                     AFGAAAVMLPGSKMPGPEQVAAIAVTVDESPDFSRPLND"
     gene            complement(13054..14097)
                     /locus_tag="PKJ61_06635"
     CDS             complement(13054..14097)
                     /locus_tag="PKJ61_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011183779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent dehydrogenase"
                     /protein_id="HOC13536.1"
                     /translation="MKVLRFYAPGDVRVEDAPVPQIASNEVLLRVRNCSTCGTDVKIF
                     HNGHQNITPPRVMGHEVAGEVAEVGADVTGWSVGDRAQVIAAVPCGECYECKKGWMAV
                     CANQTSIGYQYDGGFAEYMVVPHAVLKVDGLNRIPEGVGFDEASAAEPFACAINAQEL
                     LGIEPGDTVVVFGAGPIGCIHIRIARGVHQAGRVFLVDVNAERLAMSADAVQPDGVID
                     ASKVDVVAEVMRLTEGRGADVIITATAANITQEQAIAMAARNGRISFFGGLPKTNPTI
                     TCDSNLVHYRQLHIHGANGSAPEHNKKALEYIASGLVPVKDLITVHLPLERALEAFDI
                     VAKGQAIKVTVEP"
     gene            complement(14097..14537)
                     /locus_tag="PKJ61_06640"
     CDS             complement(14097..14537)
                     /locus_tag="PKJ61_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002517867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOC13537.1"
                     /translation="MTNEGILAPASVRLGLASMTKADAIRLCGQVLVEAGAATPEYID
                     GMFAREEQVSTYLGEGVAIPHGTNEARQYITAAALGFLQFPDGIDWDGNEVKLCIPIA
                     SASDDHLGILASLAGVLMEPDAAERLRTTSSVEEVLELLSPEEN"
     gene            complement(14821..16290)
                     /locus_tag="PKJ61_06645"
     CDS             complement(14821..16290)
                     /locus_tag="PKJ61_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002517878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HOC13538.1"
                     /translation="MTTAVTAKPGSTRVAVQKFGTFLSGMIMPNIAAIIAWGIITALF
                     IEKGPFPNAAIATLVGPILTYLLPSLIAYTGGRMVYDTRGGVVGAVAAMGVILGCSDP
                     TLLGDRPGSTMFLGAMIMGPLTAWLMKKIDSIWDGKIAPGFEMLVDNFSAGILAAVMA
                     VASLFLLSPVMLTIMAWLSAGVGYLIANGLLPLTSVFIEPAKVFFLNNAINHGVLTPL
                     GIEMAAKTGKSVLFLLEANPGPGLGVLMAYTFFGKGAAKASAPGAAIIHFFGGIHEIY
                     FPYVLMKPILILAMIAGGMTGVFLNVALNVGLKAPSAPGSILAVAAQTADGNYLGMLT
                     AVLGSAAVTFVVAWLLLKVGKKDADEGDLSAATAEMEALKGKKSSVAGYLTGAATRPI
                     NTIVFACDAGMGSSAMGASVLRKKIQAAGFDVKVVNKAIANLDDSYEVVVSHQDLIDR
                     AKQKTPSAVHVAVDNFIGSPKYDEVVEMVKQANGAPKTS"
     gene            complement(16580..18589)
                     /locus_tag="PKJ61_06650"
     CDS             complement(16580..18589)
                     /locus_tag="PKJ61_06650"
                     /inference="COORDINATES: protein motif:HMM:NF036966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOC13539.1"
                     /translation="MRTAAVVMAAVLAAGCTTGPPTGGSPAPVTSAPTTSAKSAVDVL
                     GDAQVVDLLAAVPQRTPKALPAAKLAEGIAPPTNRWYSGLVFSGQPVFPLPLSFALTD
                     DGFAFGLPVVKPTETTIFGGHAAQVSVAVGAKTSVVTSDDPSVVIVEQRDAEGAAIGH
                     ATIAQGSPFVSWTATKAAKLTIPSGFADVGNGLYVATLNGTRYALAGHDLKVDGTSVA
                     VPADGTLAFWPLPEGREPAELVDRALATVTGSSVGYEVADDEVTTTLSYATTAPTAIA
                     RMPHQASGGECALGSYPSIYGALELCDGSSLTWTTPRTPALASLDLSGISAEQKAKLV
                     EQLQADADLPDFPADSYFGGKSLQRAAMLVMVADQLGEQAIADELAARLDEQLSRWLE
                     PNGCAEREAMCFVYDPAGKGMIGLTPSFGSDEYNDHHFHYGYFLYAAGVLAARDASAV
                     SRYGPVLNLLAADIASSGGESFPQRRAFDVYASHSWASGTAPFADGNNQESVSEAVNA
                     YAGLNLWAQASGDRALADESAWMLSAEADASTRYWTNPELSGTEFAGFGHQIVALNWG
                     GKRDYATWFSAEPAAMLGILLLPMSPSSGYLAGDPERIRANVAEAVGEVADQKFGDYI
                     AMYSALAGPEDRDRAVVAAEKLSDGTIDDGLSRTYLLAWLYSLKF"
     gene            complement(18586..19344)
                     /locus_tag="PKJ61_06655"
     CDS             complement(18586..19344)
                     /locus_tag="PKJ61_06655"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HOC13540.1"
                     /translation="MTWENRFRLLGGVTAVIALVLGLAMVFNHRQNEITSHLALVAAD
                     TYTVGADYAGTVTRALVKEGDAVAKGEQLFTVQSLQLKEAMANGLEIDDTEAYKVDAK
                     RGTLTYYAVISGTVDELHAQQGNSVPASSPLATITGGERYLEASFHVVPRDYARVQPG
                     SPARITLPNDQVITGSVEKVTVSSDPDATADAGPNTTVTTLRLKSSQLANLTPQPLSE
                     PGTPVIAVVQLADTGPLAPLTDAMNDLLQQVGLR"
     gene            complement(19341..20894)
                     /locus_tag="PKJ61_06660"
     CDS             complement(19341..20894)
                     /locus_tag="PKJ61_06660"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015070393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOC13541.1"
                     /translation="MILLTIIATGGILAYAIFLLNPANRGDTLPYVLVIGAEAILIAH
                     ALLAMWTILAGAKSARDHAYYAAQSSLLEAGRDDAPLLINGQPASVAVFITVYGEPLD
                     VIRRTATAARGLRGEHSTWILDDGRSDDVRDLAAELRVGYLRRLSSGGAKAGNVNHAL
                     SQVKTDYFCIFDADFAPLHDFLYETMPFFADADVAFVQTPQSYGNLRNFVSRGAGYMQ
                     TVFYRFVQPGRNHFNAAFCVGTNVVFRRTAIDDVGGIYTDSKSEDVWTSLMLHERGWR
                     SVYIPDVLAVGDAPETIEGYTKQQLRWATGGFEILFNRNPLHPKRALTLDQRLMYLVT
                     ATHYLTGIAPGILLMVPALEVFFDLRPVTLGTSMWEWFFAYAGFYVMQILLASFTLGS
                     FRWEVLMLATASFPIYHQALLNAFSGKEQQWHVTGASKRKSSPFNFIRPQVYLFVFLA
                     LTSVVAIWRDLNNAQFSLATAWNLTNTAVLGAFLVVAFAEHFAPDPARVQARSPRRVR
                     LATAEGATP"
     gene            complement(21221..22504)
                     /locus_tag="PKJ61_06665"
     CDS             complement(21221..22504)
                     /locus_tag="PKJ61_06665"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012924253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HOC13542.1"
                     /translation="MPGIVVVGAQWGDEGKGKATDQLGDRVDYTVRYSGGNNAGHTLV
                     VNGEKYALHLLPSGILSPTSVPVIANGVVIDLEVLFQEIEGLEARGVDTSKLLISANA
                     HIIAPYHKVLDKVTERFLGNRRIGTTGRGIGPCYSDKINRLGIRVQDLLDSSILAQKV
                     EAALDQKNHLLVKVYNRRAIDPAEVTEDLLTYVDRVRPMVTDVVRVLNDALDADKVVL
                     FEGAQAHHLDVDHGTYPYVTSSNPIAAGACIGSGVGPTRIDRTIGIMKAYTTRVGEGP
                     FPTELLDADGEKLRTDGAEFGTTTGRNRRCGWFDALVVEAAVAANAFTDMFLTKLDIL
                     TGWEKIPVCVAYEVNGERTNRLPMTQTDFHHAKPVYEYLDGWTEDISGCREFTEFPAN
                     TQAYVRRLEELVGARISGIGVGPSREQVVMLHDLL"
     gene            22762..23616
                     /locus_tag="PKJ61_06670"
     CDS             22762..23616
                     /locus_tag="PKJ61_06670"
                     /inference="COORDINATES: protein motif:HMM:NF016370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HOC13543.1"
                     /translation="MHPEIAYILAENGAIATKQHPNLGSTLRRLHLHGDLDNPLPGVF
                     VAPGQYHGVTRLRAVSLWSAPLGVLHERTAAGIWLPGIETPLVRLAHPTLRSRSRVHI
                     TRRQIPREFIRSAKGLRTASAAYVAAELAAFDDGRAATEVLRLKLATPDELRQATRTL
                     AATVGHEQRTRVIAACADNPWSYAELRLHRILRETGITGWVANRPIRIGSQVFDPDIR
                     FLKRRVIIEVDGRETHLLTRQFLLDRQRQNAFTVADFRVIRFTWEQLDDPAYVITVVR
                     AVLALACR"
     gene            complement(23747..>24216)
                     /locus_tag="PKJ61_06675"
     CDS             complement(23747..>24216)
                     /locus_tag="PKJ61_06675"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002520672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HOC13544.1"
                     /translation="DGLRAIEVLGLGEKGRYMTALTFGNVHGVYKPGAVKLRPEVLKE
                     IQDAVGAKYGREKPFDLVFHGGSGSSPQEISDAVDFGVVKMNIDTDTQYAFTRPVVDW
                     MLKNYEGVLKIDGEVGNKKQYDPRAWGKTAEAGLSARVVEACQYLRSVGKTIG"
BASE COUNT         4031 a         8345 c         8056 g         3784 t
ORIGIN      
        1 ctaagctgag ccccatgctg atcattggcc tggtgctgtt tggaatgctc gtcggagccg
       61 ccgcccaact cattctgggg tcgtcccgga aaggcatcga ctgggcgttg gccttcgtca
      121 gcggcctggt tggctccttc gtcggcggct tgctgatcag cctgctgagc ggcgacgggc
      181 tggagctgcg gccgtccggc ctgatcgggt cggtggtcgg agcggtgatc gtcaccgcga
      241 tctggcagtg gcggcagcgg cagagccgct agggctgctg tgcccacacc acagcacggc
      301 aggagtggct ggtgagccaa cccgtcaccc gcgccccgct ggtgttgatc gtcgaggacg
      361 aggtgccgct gcgggagtcg gtggctcgac acctggccgc gtccggtttc accgcagtgg
      421 aggccggatc ggtcgccgcg ggactggccg cagcgtggga gcaccagccg gacgtggttc
      481 tgctcgacgt gctgctgccg gacggctccg gctacgacct gtgcgccaaa ctgcgcccga
      541 tcaccgcggc gccgatcatc tacctgaccg ccctcggcca ggaccgcaac gtcgtccagg
      601 gcttggccac cggggctgac gactacatcc cgaagccgtt cagccttgag gtgctgaccg
      661 cacgcatcca ggcgcagctg cggcgcacgt ccgctccgcg caagggccga atcgacctac
      721 caccgctgca cctggactat ctcaccggcg aggtgctgct ggatggccgc gagatcaatc
      781 tcaccaagat ggaactgcag ttgctcggct tcttcgccac caacgtcggc gtcggtgcca
      841 cccaggccca actgctcacc tcggtgtggc gctcgaaggc ggacatcccc acgaacaccg
      901 tccgccagac cgtctcatcc ctgcgccgga agctggccct ggacgccggc agcgcgttcg
      961 aactcgaatt ctccgcggac aagcgctacg tcttccgcca ggtgcgtttc gacccttcgc
     1021 ccacctgaac cggaaagcga cccaccccgt cccgtttccg ttcgccgaca gtgtggagga
     1081 ttgtgcaccg aactcggtgt gcaatcctcc accttgaccc gaaccgtccc tctttgctca
     1141 ggtgaggggc agggtgaggc ggactgtggt gccgtggcct ggggtggagg cgacgtgaag
     1201 gtcgccaccg tgggccactg cgatctcgcg gcagatcagt agaccgacac cgaggccggt
     1261 atcccgggcc gagcggacgc ccgactggtg cgtccgcacg ggttggcgac cgagctggtc
     1321 gaggaggtcg ggcgcgatgc cctcgccgga atcggcgacg atgagctcgg cgaagccagc
     1381 ggcctcgccg acgcggacgc tgatcgtccc gttgtgggtg tgccgggatg cgttcgagag
     1441 caggttgacc agcacctgga tcacccgatc ccggtcagcc agaacggtgg gcgtcgcacc
     1501 gcctggcggc accacgagcg tgttcccgtg ctgggcgaag atcggacggt acgcctgcag
     1561 cgtctgctgc accagcgcat ccagggcgag cggacgcagg tcgacgtcca gccggcgttc
     1621 gctgatacgg ccgaggtcga gcagctgggt caccagatgc gccagccgat cggtctccga
     1681 accgatcacc ccgaggtcgg cgactacgcg cgacgtgccc gcgtccgctc gcgcattcgc
     1741 gtccgtcgtg gccagggcca gtccggcctc gtcggtgtgc gtgcgaatga ctgccagcgg
     1801 ggtgttcagc tcatgcgcga ccatcgccaa gaactcggcg cgagtggcat cgagcctagc
     1861 cagctcagcg ttgcgcgcag tgagttggtc cttgttgcgc tggtagaggc ggattaacaa
     1921 gcgcagcgcg atcgccaggg ccaggccggt ggcgagcatc gagtagatga tgtcgacgaa
     1981 cttcgcgaac gccgacggca gcggcacgac caactggggg tagctgaacg cgagcaccag
     2041 gctggtcgcg aacacgacgg cctccagcgg gaccatcacc agcagcgcgg gaccttcgag
     2101 gatgatcgct gagaacgcca gggcgaaggc gaaccagatc ggcatgccgc cctcccagcc
     2161 gccgccggcg aagaacaggc cagggaagag cacgacgaac agcgcgccga ccgcgatcgc
     2221 gtaggtgagg cggtagcgtc cgctgacccg tccgaaccaa accaggccga ggatggcgag
     2281 cccagcgaag aagttcagca gcaccgtcca gaccgggcta cccagcacga cgttgatcaa
     2341 cgcggacgcc cagctgatcg gcaccccgac catcgcggtg accgcgatca gttgctcacg
     2401 gaagtcctca cccgcgcgcg gcggcaggct gccgggcagc cggcggagcc agcgccacgc
     2461 aggtgtctcc gtcatggcca gaccctactt ccaaaggtgt gggcgcaatt caactccggc
     2521 gactcgccca cggccgcgaa cgataaaaag cctttgatcc gcggccgccg ccccacctcg
     2581 catcgacagg ctggcgctta ctcggtcgcc gagcactacc gatgtcgtga ccatcaccac
     2641 cggtccggca aaggcggtga tcgacgcgct cccgcggggg gagcatcgcc ggcccgctgc
     2701 gcggggcact cggggggatg ccccgcgcag ccggtctggc taggctgatc gcgatgtctg
     2761 tcccagccga aatcttaccg atcaacgtcc gaccggagcc cttcagcgtc tacaccactc
     2821 ctgaattctg gaacgaccct cacctgtcgg cgcagctgtt gcaagcccac cgtgacccgg
     2881 attcgggccc tgcctcgcgt ccgcacgcgt tcatcgagcg gtccgtggaa tggctgctcc
     2941 aagcactcgg gctgcgtccg ggcagccggg tgctcgactt gggctgcggc ccgggcctgt
     3001 acgcgaaccg cctggctggg catgggatac aggtgaccgg tatcgacgta tcccgtagtt
     3061 cattgacgta cgcccggacg ggggcgcagc agtctggccc ggcggcggag ttcatcgaag
     3121 gcaactacct tgaggccgac ctgggatccg ggtacgacgc cgctctgctg atctatgcgg
     3181 acgtctgcgc gctgagcttc gaacagcgaa ggtccttcct gcagcgggtg ctgtcggcgc
     3241 tgctcccggg tggactgttc gccttcgacg tgacggccgc gccacgcttc acccactacc
     3301 acgaaggcat cgaactgctg ccggccgacg gcttctgggc ggctggcccg cacgtcgcga
     3361 cccgggccac ctggacctac cccgagctgc gcctggtgct ggagcgctac gtgatccaga
     3421 cgccgacgaa ggtgcgcgag ttctggaact ggacgcactg cctgaccgca tttgaagtgg
     3481 aggccgaact ggcgtccgct ggtttcgccg atttccggcg gttcggcgac gtcgccggcg
     3541 ccgactacga cccgggcgcc gacaccttcg cgctgattgc ccgccgccca ggctgagcgg
     3601 cacccggacc ggtcacgcag caggcggacg cagcgcttcg cgcgccatcg tcccgccgac
     3661 gaacagcagg aagccggcga acagccaggc agccggttgc ggcggaatcg cgtgcgccac
     3721 ccagacaccc agcgggctgg agaccacggc ggctgcaccg atcatcaggc ccagccggac
     3781 gtccacgaac tgcttgcgca ggttcgccac caacccggac gcgacccccg gcagcatcat
     3841 gaccagcgaa gcgcccttcg cgctcaggtc accgatgccg aaccagacca tcagcaccgg
     3901 caccaccacg ccgccgccac cgaccccgag cagcgcggac agcacgccgg cgcccaggcc
     3961 gagcaccacg agcccgatac cgcgccaaac atcgatcggc aggtcggcgc cgcgttcggg
     4021 aacgagggac agcaactgca cgatcatcac caccacaaag ccgacgaaga tccattgcgc
     4081 agcgcggcga ctgatccgtc cgagcagcca gactccggcc tgggcgccga cgatcgcacc
     4141 aaccgcgagc agcccaccga ccgcgaggtt gaccgagccg gtcgcagcgt aggaaaccag
     4201 cgaagccgtg accgcaggca cgatcgccag cagcgaagtg ccgaccgctc gccggtggtc
     4261 gagcccgagc agcaccgcca acgccggcac cacgatcagg ccaccgccaa ccccgaacag
     4321 cccggagaag aagccggacg cgagcccgat tccgattgcg atcagaacta gctgattgcc
     4381 ggacggcacc tggcggcccg ccgcgacgcg ctcccccggc tctggatccg aggtcacagc
     4441 accgcgccag ggcggtaggc cgcaccctcg gggaagcgga cggccagctc gcccacccgg
     4501 gcggcgatcg tgtccacctg gtcacgggcc gagccggtca gctccagcgg atcgaccacg
     4561 accgcgtcca gctgctcgcg ggtcaggccg aggcgcgcat ccgcggccag ccggtcgtag
     4621 agatcgttcg tggccgctcc cgcgcgcatg gccagcgcga ccgccacggc atgctccttg
     4681 atcacctcgt gcgcggtttc gcgtccgacc cctgcccgaa ccgcagccat caggatcttc
     4741 gtcgtggtca ggaatggcag gaagcgctgc agttcggcct ctaccaccgc cgggaaggcg
     4801 ccgaactcga cgagcaccgt gaggaaggtc tcgaacatgc cgtcgagcgc gtagcacgcg
     4861 tccggcaagg caacccggcg gacgaccgag caggacacgt ccccttcgtt ccactggttc
     4921 ccggccagct caccggccat ggacgcgtag ccgcgcagca ccaccgcgag cccattgacc
     4981 cgctcgcacg accgggtgtt catcttgtgc ggcatggcgg acgagcccac ctggccctcg
     5041 gcgaagccct cggtcaccag ctcctgcccg gccatcagcc ggatagtcgt cgccaggctg
     5101 gacggaccag cggccacctg caccagcgcg gtcagcacgt cgtagtcgag cgagcgcgga
     5161 tagacctgcc cggtcgcggt gaacacgcgc gcgaacccca ggtgctcagc gaccctccgc
     5221 tccagctcgg ccagtcggac ggcgtccccg tccagcaggt cgagcatgtc ctggccggtg
     5281 cccatcggac ccttgatgcc gcgcgcgggg tagcggccga tcaactcgtc gactcggtcg
     5341 aaggccacca gcaattcgtc agcagcggac gcgaaccgct tgcccagcgt ggtggtctgc
     5401 gcagccacat tgtgcgaccg gccagccatc ggcaggtcgc ggtactgcac cgccaaccgg
     5461 gccagattca ccagcaccgt gacaatacgc tcgcggatca gctccaacga cgacagcaac
     5521 tgccgctgtt cgatgttctc ggtgaggtca cggctggtca tgcccttgtg gacatgttcg
     5581 tgcccagcga gcgcattgaa ttcttcgatc cgcgccttca catcgtgccg agtgacccgc
     5641 tcacgggcgt tgatgctggc caggtccacc tggtcgagca cgcgctcata cgccgcgatc
     5701 accgcgtccg gatcgtcacc accgaagtcc acgccaagct cggcctgggc ccgaagcacc
     5761 gcgacccaga gcttccgctc ggcaatgatc ttcgcctccg gcgaccagat ctcctgcatg
     5821 gcggtggacg cgtaccgcgt cccgagaacg ttggggatca tgcgtcactc cctgagcctg
     5881 ttgacgagcc ggcgtcctga acggaaaagg gactgtcccc ttttcgttca gcatcggcgg
     5941 ccgccgcagc gatgtcggtg cgatggaagc cacccggcaa agtgatctgg gccaccttgg
     6001 cgtaggcagc ttcccgggct tcagccaggg tgtcgcccgt gcccaccacg gtcaggacgc
     6061 gaccgcccgc gctcaccagt tggccggacg ggtcctgcgc ggtcccggca tgaaagacgc
     6121 caccgagttc agcaccagcg atcgggtcgc ccttgcgcgg tgcgtccgga tagccggccg
     6181 ctgcgatcac cacacccacc gcggcgccgc tgccccactc caggctgcgg ccagccagtt
     6241 cacccgtggc cgctgcgtac agcaactccc ccagcggtga cttcagccgg gtgagcagcg
     6301 gctgggtctc cggatcgccg aagcgggcgt tgaactcaac cacccgcagc ccgcgcgaag
     6361 tcagcgcgag accggcgtac accaaaccga tgaacggcgt cccccgccgg cgaagctcgt
     6421 ccacggtcgg ttggatgacc gtccgcacca cctcgtccac cagcccggac ggagcccacg
     6481 gcagcggcgt gtaggcaccc atcccgccgg tgttggggcc ggcatcacca tctccgaccc
     6541 gcttgaagtc ctgcgccggc tcgaacggca ccacgttcgt tccgtcgcta agcgcgaaca
     6601 ggctgacctc tgggccgtcc aggtactcct cgatgatcac ccgccgacaa ctcgcggcgt
     6661 gccggatcgc ggcgtcccga tcgtcggtga ccagaacccc cttgccagcc gccagtccgt
     6721 cgtccttgac cacgtacggc gggccgaact cgtccagcgc gtcgatcacc tcgcccatgt
     6781 cgagacacag ccgcgcgtcc gcggtcggga cgccggccgc ctccatgatc tccttggcga
     6841 acgccttgct gccttccagc cgagccgcct cggcggacgg gccgaagacc gctatcccgc
     6901 gcgcccggac ggcatccgcc accccgacca ccagcggcgc ctcagggccg accacgacga
     6961 gcgttgcacc cagttcgacg gcgagcgcgg cgacggcatc cccgtccaga gcatccaccg
     7021 ggtggttcgt cccgaccagc gcagtacccg ggttgccggg cgcgacgtgg accgcaccca
     7081 ccgaggggtc acggctcaac gaccacgcca aggcgtgctc gcggccaccg gatccaatca
     7141 ccagaacggt ctgcgtcacc ccgcgatcct atcggcgggc tgatgtcaga cccggcggcg
     7201 ttcgcgggag gtacgattcc aggactttac cggcaagact ctttacagtt atgttcagcg
     7261 catgcctgcc agtccggtgc cacgaaacct cgcccagatc gcggacatgc tggcgaatta
     7321 cgggacgata gccgccgagc atcgtttcga cgaagggtga catgagcatc gacaccggca
     7381 cgcccacctg gctgcgcacc tacaacgacc gcgccgcctt ccgtctcctc cttgagcacg
     7441 ggccgctgag tcgctccatg ctcggcaagc tctccgggat gtcgaagccg acggccggcc
     7501 agatgatcac ccgtctggag cggctgggcc tgatctcgcc ggtgggcgag gtgaccggcg
     7561 cccgtggacc gaaagcgacc gcgtacggcg cccgcgccga ctccatgacc ggagtggcgg
     7621 tcagcatcct cgccgaccgg atcgaagcag tgctggtgga cccgaccgac gccgggcatc
     7681 cgattgcgga cctgtcgatc gagggcgtgg agcgctcacc ggtcactgac gtcacccgag
     7741 ccgtggacgc ggcctgcgcc accgcaggag tcgaccgggc gtcggtgagt gtggttgccg
     7801 tcggggtcca ggccgcggtg gacgccgaca ccgaccggct caccttcacc gacaccctgc
     7861 ctggatggcc ggagcgcggc gcccgcgccc tgatcgaggc cggcaccggc ctgacgacca
     7921 cggtcgagaa cgacgtgaac ctcgccgcca tggccgaacg ggcgatcacc gacgaagccg
     7981 acttcgtgtt cctgtggctc ggctccggtc tcggcacggg catcaacgtg gacggagtcg
     8041 tccgtcgcgg cgcgtccggg agcgcaggcg agatcggcta cctcgaagtc ccccggtcgg
     8101 caatcgcact cgacccggcc gccgaaacct tcaccgacct gctcggcgga ccggcgatcg
     8161 ccaggctgct gggccacccg gccgccgatt tcaccgccgc cctgaaggaa ctcccgcaga
     8221 acgagccggt gctcgcggag ctcgcggttc gggtggtgat gctggttgat gtcatcaccg
     8281 ctgttctcga cccggcctgt gtggtgctcg gcgggccgac cgggctggcc ggtggcgatc
     8341 ggctcgccga actggtgcag gcccgcgtcc gggcaagccg cgcgggcgac gaacccgtcc
     8401 tccagggcgc ccggcaactg ctcgtagcca acatcaggga acgactggag gcggccatcg
     8461 ccgcctcctg atttcaccca ccgtcatccc gcgactcacc caccgtcatc ccgcgactca
     8521 cccaccgtca tcccggcgaa ggccggaatc gccaaaggag aatcatgaag ctcgccatcg
     8581 tcggcggagg ttcgacctac accccggaac tcatcgacgg gttcgcccgc tacgccgacg
     8641 tactgccgtt gagcgagatc tatctcgttg atccggacgc acaccgactg gagctggtca
     8701 ccggcatggc acagcgaatg ctcgcgcgtg cgggcaatcc ggcgaaagtg gtgggtacgg
     8761 ccaacctggc cgagggtgtc gagggcgcgg acgcggtgct ggtccagctg cggatcggtg
     8821 gtcaggcggc ccgcaaagcc gacgagacct ggccacacga ggtgcactgc atcggccagg
     8881 agaccacggg gccgggcggc ttcgcaaagg cactgcggac ggtgccggtg atgctgcaga
     8941 tcgccgaagt ggtcgccgag cgggccaagc cggacgcctg gctgatcgac ttcaccaatc
     9001 cggtgggtat cgtcacccgg gcactgctgc aggccggcca tcgggcggtc ggcctgtgca
     9061 atgtggccat cggtttccag cgccggttcg ccgcggtgct cggcgtccag cacgatcagg
     9121 tgagccttgg tcacgtcggg ctgaatcacc tcacctggga gcgcagcgtg accgtcgacg
     9181 gcgtcgaccg gctgccggaa atgctcggcg gcggcctggc agacatcgcc gaagaggtgg
     9241 aattgccgcc ggcgctgctc gcccagctgg gcgtcgtgcc ttcctactac ctgcgctact
     9301 actacgccca tgacgaggtg ctcgccgagc agctcgccgc acccacgcgc gccgaggccg
     9361 tccaagcaat cgaggacgaa ctgctgaagc tgtacgccga cccgtccgtg gacaccaagc
     9421 ccgagtcgct ggagaagcgc ggcggggcct tctactccga agccgcggtg gaactcctgg
     9481 cggcgatgcg cggcgcagcc ggtccgtccc gggtggtcaa cgtgcgcaac gacggactgc
     9541 tgcctttcct gcctgcggac catgtggtcg aggtgccggc acagttccgg gacggacgct
     9601 tcgtcgccga cccggtcgcc ccgctgcccg acgacgtcgc cggcctgatc gccgcggtcg
     9661 ccggctacga gcggctggcg ctggacgcgg ccgtccatgg cggccgggat cgagtgctgc
     9721 gggccatgcg ggcgcatccg ctggtgctgc agcacgaccg cgccgagcgg ctgaccgacc
     9781 tgctgatggc cgaaaacgca cggtacctac cgtgggcgtg aatgaggtcg tcgtcgcggt
     9841 cgacggcggc ggcaccaagg cggacgcggt tgcgttcgac ctgaccggcc ggctggtggc
     9901 cagcagtcgc ggcccgggtg gcaacccgca gtccgacgga gtcgcggcgt ccctggcgat
     9961 ggtggacggc ctcattcgcg aagtcgccgg ttctgcgcgg gtgctacggg ccgggctgta
    10021 cctctctggg ctcgacctgc cgcaggaggt gaccgcctac cgcgaggcgg ccgcgaactc
    10081 gccctgggcc gcgtccgggc tggacgtgga caacgacctg ttcgcgctgc tgcgtgccgg
    10141 caccgaagag ccggacgcgg tcgcggtggt ctgtgggacg gggatcaacg ccgtcggcat
    10201 ccgtgcggac ggcgccacgg cccgtttcct cgcgctcggc ccgatctccg gcgattgggg
    10261 cggcggcctg ggccttggcc aggaagcgct ctggtacgcc gctcgtgacg tcgacgggcg
    10321 cggcccgcat accgcgctga ctacggcaat cagtacgcat ttcggcgtgg acgtgagtgg
    10381 cctgatcacg caattccacc tcggcgagcg cagccggagt gaactggccg agctggcacc
    10441 cgcggtgttc gcggtggccg tggccgggga cgaggtcgcc ggctcgctgg tggatcggca
    10501 ggccaaggag atcgtcgacc tggctcgagc cgccctgacc cgcttggagc tgacccactc
    10561 ggcagtgccg atcgtgctgg gcggcggcat cctccgcgcc ggcgacgccc ggctcctaga
    10621 cggcatccgc accggcctgg ccgaggtcgc cccgaccgtc cgcctggttc acgtcaccaa
    10681 gccaccgatc ctaggcgccg cgctgctcac tctcgcccac gccggcgccg acacggagac
    10741 gttggcccgg gcccagaccg cccttctcta gaatcccgcc aacgatggcc gagcttcggc
    10801 gctacgcaat cgggcgaccc acgggttgac gcacgggacg cccagcggct caaagtgctt
    10861 gaggttacgg gtcacgacga ccatttagtt cgcttccgcg atggctgcac caggtcggcc
    10921 tgcacccgca gcagccagaa caggaagcgc gctgggctgc gagtgtcggg acggctcgtc
    10981 tcgatcgggt gtaggcgtcg acgaggggcc ggaaatcatg cgtggccgac gaacactacg
    11041 ccgatccgcg gccgctgtca ggcagagtaa tgcccgattt tctgagcaat catgtggaat
    11101 ttgctgcgtt cgtgtggatt tgtgcgtatc ttggttccat ggccgccaac gtcgacgctg
    11161 aggacacccg cctgctgccc catgagcggc acctgcgcat cctcgagcag gcgcgcagtc
    11221 ggtcccgggt cgaggtcgcc ttgctggctg acgaactcga agtcacgtcg gagactatcc
    11281 gacgagatct caccatgctg gaaagacagg gcctgctgag gcgcgtccat ggcggggcgg
    11341 tacgcataga gcggctgggg tacgagccgt cggtgaacgc ccgcagagag catcgagtgg
    11401 ccgagaagtc gcggatcggc cgcgctgcgc gtgacctgct gcctgatggc ggctcgatcc
    11461 tggtcgacag tgggacgacg acgctcgaac tggtgcggaa cctgccgtcc gatctgccgc
    11521 tgaccgtggt cacgaattcc ctgcaggccg ccgtggtcct cggcgactac ccacgggtcg
    11581 agctgctgct gctcggtggc cacgtccgcg agctcaccgg agccgcggtt ggcacctgga
    11641 cgacgggagc gctggcccac ctgcgtgtcg acgtcgcctt catggggacg aacggcgtga
    11701 cccctgagct cggcctgacc actcccgacc aggccgaggc cgaggtgaag cgggcgatgg
    11761 tcaaggccgc caaccgggtg gtctgcctcg ccgaccacac caaggtcggt ctcacccagc
    11821 tgtgccgttt cgccggcctg gacgaagtcg acgtgatcgt aaccgaccgc ggcctggagg
    11881 ccgggctcgc cgaagaactc accacagccg gaccggaggt gatccgcgca tgatcgtcac
    11941 gctgaccccc aacccaagcg tcgaccgcac catttccgtg gccacgctcg agcgtggtgc
    12001 ggtgatgcgc gcgaccgcgt cccgggaaga tcccggcggc aagggcctga acgtgagccg
    12061 cgcgctcgcg ctcaacggca ccgtgaccgc agccgtactg ccggtcggag gcgcgtacgg
    12121 acggatgctg ctcgcgctgc tcgaatccgg cgacgtggac gtacgtccgg tgccgatcgg
    12181 ggcagcaatc cgcgccaacg tggcgatcgt ggagccggac gggaccacga cgaagctcaa
    12241 cgagcagggt ccacagctgc accccgccga gctggccgcg ctccggcagg cagtgctgga
    12301 cgcggccgcc ggagccagct gggtggtggg ttgcggctcg ctgccgcccg gtgtcgacga
    12361 cagtttctac gccgagttgg tcgggctgtg ccacgccaac ggcgtccgcg tggccgtgga
    12421 ttcgtccggt ccgtcgatgg ctgctgcgct agcggcggcc ccagacctga tcaaaccgaa
    12481 ccggatcgag ctggccgagg ccgtcggccg cgacctggac accgtcggcg cagtggtgga
    12541 cgcgagccgc gagctggtcg ccagcggcgt cgggacggtc gtggtgagcc tcggccgcga
    12601 cggcgcgctg ctgatcgacg ccgagcgggt ggtgcacgcc gtggcgagca tcaaccaccc
    12661 gctttcgacc gtgggcgccg gcgattcact gctggccggc tacctgcacg ccgtggacgc
    12721 gggcgccgac ccggtcgcgg cactgcggac ggcggtcgct ttcggcgcag ccgcagtgat
    12781 gctgcccggc agcaagatgc ccggccccga gcaggtcgcc gccatcgccg tgaccgtcga
    12841 tgagagtccc gacttctccc gtccgctgaa cgactgatcc cggcggcgac ggctgcctca
    12901 gtctcgtcgc gcgatcggcc tcaaggcgga cgcttcctcg ccgtcagcgc gcgaaaccgt
    12961 ccaccttgct gctgggtcgt cgaccagaac acaaagtggg cagtaatcgc cgaagtcgac
    13021 gattactgcc cactttgcgg acaaccggga ggatcagggc tcgacagtca ccttgatcgc
    13081 ctgacccttg gcaacgatgt cgaacgcctc cagcgcacgc tccagcggca ggtggaccgt
    13141 gatcaggtcc ttcaccggca ccagtccgga cgcgatgtac tccagcgcct tcttgttgtg
    13201 ctcgggcgcc gagccgttcg ccccgtggat gtgcagctgg cggtagtgca ccaggttgga
    13261 gtcgcaggtg atggtcgggt tggtcttcgg caggccgccg aagaacgaga tccggccgtt
    13321 gcgggcggcc atcgcgatgg cctgctcctg ggtgatgttg gccgcggtcg cggtgatgat
    13381 cacgtccgcg ccccggccct cggtgaggcg catgacctcg gccaccacat ccaccttgga
    13441 ggcgtcgatc accccgtcgg gctgcaccgc atccgctgac atcgccaacc gctcggcgtt
    13501 cacgtccacc aggaacaccc gtccggcctg gtgcacgccg cgagcgatcc ggatgtggat
    13561 gcagccgatc ggaccggcac cgaacaccac gaccgtgtcg cccggctcga tgccgagcag
    13621 ctcctgggcg ttgatcgcgc aggcgaaggg ttcggccgcg gacgcctcgt cgaagccgac
    13681 gccttcgggg atgcggttga ggccgtccac cttcagcacc gcgtgcggca ccaccatgta
    13741 ctcggcgaaa ccgccgtcgt actggtaccc gatcgaggtc tggttcgcgc agaccgccat
    13801 ccagcctttc ttgcactcgt agcactcgcc acagggcacc gcggcaatca cctgcgcacg
    13861 gtcgccaacc gaccatccgg tgacgtcggc gccaacttcg gccacctcgc cggcgacctc
    13921 gtgccccatc acccgcggcg gggtgatgtt ctggtggccg ttgtggaaga tcttcacgtc
    13981 ggtgccacag gtcgagcagt tgcgtacccg caacagcacc tcgttggacg cgatctgcgg
    14041 cacgggcgcg tcctcgaccc gcacgtcacc aggcgcatag aagcgcaata ccttcatcag
    14101 ttctcctccg gactcaacaa ttcaaggact tcctcaaccg aactcgtggt gcgcagccgc
    14161 tccgccgcgt ccggctccat caacacaccg gccaggctgg ccaggatgcc caggtggtcg
    14221 tcgctggcgg acgcgatcgg gatgcacagc ttgacctcat tgccgtccca gtcgatgccg
    14281 tccgggaact gcaggaagcc cagcgcggcg gcggtgatgt actgacgcgc ctcgttggtg
    14341 ccgtgcggaa tggccacgcc ctcgccgagg taagtggaca cctgctcttc gcgggcgaac
    14401 atgccgtcga tgtactcagg cgtggccgcg ccggcttcga ccagcacctg gccgcagagc
    14461 cggatggcgt ccgccttggt catggatgca agcccgaggc gcaccgaggc gggcgccaga
    14521 atcccctcat tggtcaacga gttctccgat cttcatggcg tcccaggccg ggccgcgtgc
    14581 gatggtgcaa catttcgatc cttctccgac tccagtgtcg agcgccgtgc agctctgagc
    14641 aagaccataa aaccacacaa acacagacga gtcaatgcaa aacaacattg ttcggacgct
    14701 tccaggccag cccagacaag ccactggatg cagcgaggcc cgcggaaacc accggttccg
    14761 cgggcctcta ctactcggcc ggcctcgtcc caacggcagg gacgagcgct acgccgacgc
    14821 tcaactggtc ttcggcgccc cgttcgcctg cttgaccatc tcgactacct cgtcgtactt
    14881 cgggctgccg atgaagttgt cgaccgccac gtgcaccgcg gacggcgtct tctgcttggc
    14941 ccgatcgatc agatcctggt gggacacgac cacctcgtag gaatcgtcga gattggcgat
    15001 cgccttgttg accaccttca cgtcgaagcc ggcggcctgg atcttcttgc gcagcacgga
    15061 cgcgcccatc gccgacgacc ccatccctgc atcgcaggcg aacacgatgg tgttgatcgg
    15121 ccgggtggcg gccccggtca ggtagccggc aacggaggac ttcttgccct tgagagcttc
    15181 catttcggcg gtggctgcgg acagatcgcc ctcgtcagcg tccttcttgc cgaccttgag
    15241 cagcagccag gcgaccacga aggtaacggc ggccgagccg aggaccgcgg tcaacatgcc
    15301 caggtagttg ccgtccgcag tctgggcggc cactgcgagg atcgaaccgg gggccgacgg
    15361 agccttcagt ccgacgttga gggcgacgtt caggaacaca ccggtcatgc cgccggcgat
    15421 catggccagg atcaggatcg gcttcatcag cacgtacggg aagtagatct cgtggatgcc
    15481 accgaagaag tggatgatcg cagcgccggg agcggacgcc ttggccgcac ccttgccgaa
    15541 gaaggtgtag gccatcagca caccgaggcc agggccgggg ttggcttcga gcaggaacag
    15601 caccgacttg ccggtcttcg cggccatttc gataccgagc ggcgtcagca cgccgtggtt
    15661 gatcgcgttg ttaaggaaga acaccttcgc gggctcgatg aagaccgagg tcagcggaag
    15721 gaggccattc gcaatcaggt aaccgacgcc ggcgctcagc caggccatga tggtgagcat
    15781 caccggcgac agcaggaaca gcgaggcgac cgccatgacg gcggccagga tgccggcgga
    15841 gaagttgtcg accagcatct cgaatccggg cgcgatcttg ccgtcccaga tcgagtcgat
    15901 cttcttcatc agccacgcgg tcagcgggcc catgatcatg gcgccgagga acatggtcga
    15961 acccgggcga tcacccagga gggtgggatc gctacagccg aggatcacgc ccattgcagc
    16021 gaccgcgccg acgacgccac cgcgggtgtc gtagaccatc cggccgccgg tgtaggcgat
    16081 caggctgggc agcagatagg tgaggatcgg tcccacgagc gtggcgatcg ctgcgttcgg
    16141 gaacggcccc ttctcaatga acagggcggt gatgatgccc cacgcgatga tggcggcgat
    16201 gttgggcatg atcatgcccg acaggaaggt accgaacttc tgcaccgcca cccgggtcga
    16261 gcccggcttt gccgtaactg cggtagtcat ctgactccct cactctttga cgtcgatggc
    16321 aatcggactc gttgtgagac ccgacgcgag gtttatacgg gaacaaaacc acacaatcag
    16381 gcacacgtca agcaaaatca acacacgctc acaggaaaac gggcacgttc tcatgcatcc
    16441 ggagtagccg cttggggagc tcggcatcgg tggatccagc cgaccaacca ctcaacgccg
    16501 gcgaaacgga cgtttgccgc cgaagattcc ctggcaaacg tccgattcgc cggcgtgaag
    16561 ctggacgggc gcgaggcgcc tagaacttca ggctgtagag ccaggccagc aggtaggtgc
    16621 ggctcaggcc gtcgtcgatc gtgccgtccg acagcttctc ggccgcgacc acagcccggt
    16681 cgcggtcctc ggggccggcc agggctgagt acatcgcgat gtagtcgccg aacttctggt
    16741 cggcgacctc ccccaccgcc tcggccacgt tcgcgcggat gcgctccgga tccccggcta
    16801 ggtagcccga actcgggctc atcggcagca ggagaatgcc gagcatggcg gccggctccg
    16861 ccgagaacca ggtggcgtag tcccgcttgc cgccccagtt cagcgccacg atctggtggc
    16921 cgaagccggc gaactccgtc ccgctgagct ctgggttcgt ccagtagcgg gtgctcgcgt
    16981 ccgcctctgc ggacagcatc caggccgact cgtccgccaa cgcccgatca ccgctggcct
    17041 gcgcccagag gttcagtccg gcgtaggcgt tcacggcctc cgacaccgac tcctggttgt
    17101 tgccgtcggc gaagggcgcc gtccccgaag cccacgaatg ggacgcgtag acgtcgaacg
    17161 cccgccgctg cgggaagctc tcgccacccg aactggcgat atcggccgcc agcaggttca
    17221 gcaccggccc gtacctcgac accgcggacg catcccgcgc cgccagcacc ccggcggcgt
    17281 acaggaagta gccgtagtgg aagtggtggt cgttgtactc gtcgctgccg aaggacggcg
    17341 tgagcccgat catccccttg ccagccgggt cgtagacgaa acacatcgcc tcgcgctcag
    17401 cgcaaccgtt cggctccagc cagcggctga gctgctcatc gaggcgggcc gccagctcgt
    17461 cggcgatcgc ttgctcaccg agttgatcgg ccaccatcac cagcatcgcc gcgcgctgca
    17521 gcgacttccc gccgaagtaa gagtcggccg ggaagtcggg caggtcggcg tccgcctgca
    17581 actgctcgac gagcttcgcc ttctgctctg cggagatacc gctcaggtcg aggctggcca
    17641 acgccggcgt acgcggcgtc gtccaggtca gcgaactgcc gtcgcagagt tccaatgctc
    17701 cgtagatcga cgggtagctg cccagcgcgc actcgccccc ggacgcctgg tgcggcatcc
    17761 gggcgatcgc cgtcggcgcc gtcgtcgcgt agctcaacgt ggtggtcacc tcgtcgtcgg
    17821 ccacttcgta gccaaccgag gagccggtca cggtggccag ggcgcggtcg accagttcgg
    17881 ccggctcgcg tccctccggc aacggccaga acgccagcgt gccgtcggcc ggcaccgcta
    17941 ccgaggtgcc gtcgaccttg agatcgtgcc cggccagggc gtagcgggtg ccgttcaatg
    18001 tggcgacgta caggccattc cccacgtccg cgaaacccga ggggatggtc agcttcgcgg
    18061 ccttggttgc cgtccaggac acgaacggag agccctgcgc gatcgtggca tgaccgatcg
    18121 ccgcaccttc ggcgtcacgc tgttccacga tcaccacgga cgggtcgtcg ctggtcacca
    18181 cgctcgtctt ggcaccgacc gcaacgctca cctgagcagc gtgcccaccg aagatcgtcg
    18241 tctcggtcgg cttgaccacc ggcagcccga aggcgaaacc gtcgtcggtg agcgcgaacg
    18301 agagtggcag cgggaacacc ggctgaccag agaacaccag ccccgagtac cagcggttgg
    18361 tgggtggggc gatgccttcg gccaacttgg ctgcgggtaa cgccttcggc gtgcgctgcg
    18421 gcaccgcggc caacaggtcg acgacctggg catcgcccag tacgtcgacc gcggacttgg
    18481 ccgaagtcgt cggggccgaa gtcaccgggg ccggactgcc acccgtcggc gggccggtcg
    18541 tgcacccggc cgcaaggacg gcggccatga ccactgctgc ggtcctcatc gcagccccac
    18601 ctgctgcagc agatcgttca tggcatctgt cagcggcgcc agcggaccgg tgtcggccaa
    18661 ctgcaccacg gcgatcaccg gcgtccccgg ctcggacagc ggctgcggcg tgaggttcgc
    18721 caactgggac gacttcagcc gcagtgtggt cacggtcgta ttggggccgg cgtccgcggt
    18781 cgcgtccgga tcgctggaaa ccgtcacctt ctccaccgac ccggtgatca cctggtcgtt
    18841 gggcagggtg atgcgagccg ggctgcccgg ctgcacccgc gcatagtcgc gcggcaccac
    18901 atggaagctg gcctcgaggt agcgctcacc gccggtgatc gtggccaacg ggctgctcgc
    18961 cggcaccgag ttgccctgct gggcgtggag ctcgtccacc gtcccggaga tcacggcgta
    19021 gtacgtgagg gtgccgcgct tggcgtccac cttgtaggcc tcggtgtcgt cgatctccag
    19081 accgttggcc atcgcctcct tcaactgcag gctctggacg gtgaacaact gctcgccctt
    19141 ggcgaccgcg tccccttcct tgaccagcgc cctggtgacc gtgccggcgt agtcggcgcc
    19201 gaccgtgtag gtatcggcgg cgaccaacgc cagatggctg gtgatctcgt tctgccggtg
    19261 gttgaacacc atcgccaggc cgagcaccag tgcgatcacg gcggtcacgc cgccgaggag
    19321 tcggaatcgg ttttcccaag tcatggggtt gctccttctg cagttgccag ccggacgcgt
    19381 cgcggcgacc gcgcctgcac ccgggccggg tcgggggcga aatgctcggc gaacgcgacc
    19441 acgaggaagg cgccaagcac cgcggtgttg gtcaggttcc acgcggtggc cagcgagaac
    19501 tgcgcgttgt tcaggtcacg ccagatcgcc accacagaag tcagggcgag gaagacgaac
    19561 aggtagacct gcggacggat gaagttgaac ggggagctct tgcgcttgct ggctccggtg
    19621 acgtgccact gctgctcctt gcccgagaag gcgttcagca gggcctggtg gtagatcggg
    19681 aagctcgccg tcgccagcat cagcacctcc cagcggaagg aaccgagggt gaagctcgcc
    19741 agcaggatct gcatcacgta gaagcccgcg taagcgaaga accactccca catgctggtg
    19801 ccaagggtca ccggacgcag gtcgaagaag acctccagcg ccggcaccat cagcaggatg
    19861 ccgggagcga tcccggtgag gtagtgcgtg gccgtgacca ggtacatcag ccgctggtcg
    19921 agggtgagcg cccgcttcgg atgcagcggg ttgcggttga acaggatctc gaagccgccg
    19981 gtcgcccagc gcagctgctg cttggtgtag ccctcgatgg tctccggggc gtcgccgacg
    20041 gcgagcacat ccgggatgta gacgctgcgc caaccgcgct cgtgcagcat cagggacgtc
    20101 cacacgtcct cggacttcga gtcggtgtag atgccgccga cgtcgtcgat cgccgtccgc
    20161 cggaagacca cattggtacc cacgcagaag gcggcgttga agtggttccg tccgggctgg
    20221 acgaagcggt agaagacggt ctgcatgtag ccggcgccgc gggagacgaa gttgcgcagg
    20281 ttgccgtagc tctgcggggt ctggacgaac gccacgtccg cgtcggcgaa gaacggcatg
    20341 gtctcgtaga ggaagtcgtg caacggggcg aagtcggcgt cgaagatgca gaagtagtcg
    20401 gtcttcacct ggctgagtgc atggttcacg ttgccggcct tggcgccacc gctgctcagc
    20461 cggcgcaggt agccgacccg caactcggcg gccaggtcgc ggacgtcgtc gctgcggccg
    20521 tcgtcgagga tccaggtgga gtgctcgcca cgcaggccgc gggccgcggt ggcggtgcgg
    20581 cggatgacgt ccagcggctc gccgtagacg gtgatgaata ccgcgacgga cgccggctgc
    20641 ccgttgatga gcagcggggc gtcgtccctg ccggcttcga gcaggctgga ctgggccgcg
    20701 tagtaggcgt ggtcgcgggc gctctttgcc ccggcgagga tcgtccacat cgccaggagg
    20761 gcgtgcgcga tcaggatcgc ctcggcgccg atcaccagca cgtagggcag ggtgtcgccg
    20821 cgattcgctg ggttcagcag gaagatcgcg tacgcgagga tgccaccggt cgcgatgatc
    20881 gtcagcagga tcatcactgg tgagtaggga tttcgcttca gttctcgcct ggtggccacg
    20941 tccgggccgg cgacttcacg taggtcatgt gttggcaata tcgactcccc cgagtcgcgc
    21001 cgcggcaggg tcttcctgcg tccgcggcat cgttggaaaa agcgaaaccg gcccgaggat
    21061 gtccctcggt ccggcgttgg cgcttccggg tacccaaccc gtccactact ccaaggtagg
    21121 cagatcgatc gccagatcac aaaccgggag cgtgttcgac acgcaaacgt aacggtgaat
    21181 taacccgtag ttcagccgcg aaaccgcggg gcccaacgac ctagagcagg tcgtgcagca
    21241 tgaccacctg ttcgcgcgag gggccgaccc cgatacccga gatccgggca ccgacgagct
    21301 cttccagccg ccgcacgtag gcctgggtgt tggccgggaa ctcggtgaac tcgcggcagc
    21361 cggagatgtc ttcggtccag ccgtccaaat actcgtaaac gggcttggca tggtggaaat
    21421 cggtctgagt catgggcagc cggttggtgc gctcaccgtt gacctcatag gccacgcaga
    21481 ccgggatctt ctcccagccg gtgaggatgt cgagcttggt caggaacatg tcggtgaagg
    21541 cgttggccgc gaccgcagct tcgaccacca gtgcatcgaa ccagccgcag cgacggttgc
    21601 gtccggtggt ggtaccgaac tcggccccgt ccgtacgcaa tttctcgcca tccgcgtcga
    21661 ggagctcggt ggggaacggg ccttcgccga cgcgggtcgt gtaggccttc atgatgccga
    21721 tcgtccggtc gatgcgggtc ggaccgacac ccgagccgat gcaggcgccg gcggcgatcg
    21781 ggttggacga ggtcacgtag gggtaggtgc cgtggtcgac gtcgaggtgg tgggcctggg
    21841 cgccctcgaa gagcaccacc ttgtcggcgt caagcgcgtc gttgagcacg cggaccacat
    21901 cggtgaccat cgggcgcacc cggtcgacgt aggtgagcag gtcctcggtg acctcggccg
    21961 ggtcgatcgc gcggcggttg tagaccttca ccagcaggtg gttcttctgg tcgagggcgg
    22021 cttcgacctt ctgggcgagg atcgaagagt ccaacaggtc ctgcacccgg atgccgaggc
    22081 ggttgatctt gtcggagtag cacgggccga taccgcggcc ggtggtgccg atgcggcggt
    22141 tgccgaggaa acgctcggtg accttgtcga gcaccttgtg gtacggcgcg atgatgtggg
    22201 cgttggcgct gatcagcagc ttggaggtgt ccacgccacg ggcttcaagc ccttcgatct
    22261 cctggaagag cacctcaagg tcgatcacca cgccgttggc gatgaccggg acactggtcg
    22321 gactgaggat tcctgagggc agcaggtgga gggcgtactt ctcaccgttt accacgaggg
    22381 tgtggccggc gttgttgccg ccggagtagc gcaccgtgta gtcgacgcgg tcgcccagct
    22441 gatcggttgc cttgcccttg ccttcgtcgc cccattgcgc gccgacgacc acgattcccg
    22501 gcatgactgc ccctcttcgt ctcagaaagc ccgcggcaat gccggcgggc tcttgcggcc
    22561 atactttacc ggcttcggcg gggaggcacg gaaggccgcc gaattcgggg ctgttcccgc
    22621 ggcacttgcg aacgtatccg cgctgcagat agctggattg ccagattccg gccaacctgg
    22681 cctatccaca gggcaaatcc gttcgccccc acccctgtgg atcactgacg cggatctcac
    22741 tcacttgccg aaggtgtggg catgcaccca gaaatcgcct acatcctcgc cgagaacggc
    22801 gcaatcgcga ccaaacagca tccgaaccta ggctcaacgt tgcggcgcct gcacctccac
    22861 ggcgacctcg acaatccgct gccaggcgtg ttcgtggcgc ccgggcagta ccacggcgtg
    22921 acccggcttc gtgcggtgtc actctggtcc gctcccctgg gcgtgttgca tgagcgaacc
    22981 gcggccggca tctggttgcc cggaatcgaa acaccgctgg tccgactcgc gcaccccacc
    23041 ctgcgctcca gatctcgggt gcacatcacc cggcggcaaa taccgcgcga attcattcga
    23101 tccgccaagg gcctgcgcac ggcaagcgcg gcctatgtcg ccgccgaact cgcggcattc
    23161 gacgacggtc gggcagctac cgaagtcctg cgactgaagc tggccacacc tgacgagctg
    23221 cggcaagcga cccgaacgct cgcagcgacc gttggtcatg aacagcgaac tcgagtgatc
    23281 gcagcttgcg ccgacaaccc ctggtcgtat gccgagctcc gactgcaccg gatactccgg
    23341 gagaccggca tcaccggctg ggtggccaac cggccgatcc gaatcgggag ccaggtcttc
    23401 gatcctgaca tccggttcct caaacggcgg gtgatcatcg aggtcgacgg ccgcgagacc
    23461 cacctgctca ctcgccagtt cctactcgac cgacaacgcc agaacgcctt caccgtggct
    23521 gacttccgag tgatcaggtt cacctgggag cagcttgatg accccgctta cgtgatcaca
    23581 gtggttcgcg cggtactggc cctggcctgc cggtgagcgg attcagccgg cagaaggctc
    23641 gtacgcgcag gcaggactct caaggccgga ctgccgcgca aatccgttct ccacagaacg
    23701 caaagcgggc cggccccgaa ggaccggccc gccaatctga ctggaatcag ccgatggtct
    23761 tgccgacgct gcgcaggtac tggcaggcct cgaccacgcg ggcggacagg ccggcctcag
    23821 cggtcttgcc ccaggcgcgg gggtcgtact gcttcttgtt gccgacctcg ccgtcgatct
    23881 tcaggacgcc ctcgtagttc ttcagcatcc agtcgacgac gggacgggtg aaggcgtact
    23941 gggtgtcggt gtcgatgttc atcttcacga cgccgaagtc gaccgcgtcc gagatttcct
    24001 gcggggagga gcccgagccg ccgtggaaga ccaggtcgaa cggcttctcc cgaccgtact
    24061 tggcgccgac ggcgtcctgg atctccttga gcacctcggg acgcagcttc accgcacccg
    24121 gcttgtacac gccgtgcacg ttgccgaagg tgagggcggt catgtagcgg cccttctcgc
    24181 ccaggccgag cacctcgatg gcgcgcaggc cgtcct
//
LOCUS       DAOPRQ010000039        23992 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_1730_length_23992_cov_12.584660, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000039 DAOPRQ010000000
VERSION     DAOPRQ010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 23992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23992
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1730_length_23992_cov_12.584660"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="PKJ61_06680"
     CDS             complement(<1..358)
                     /locus_tag="PKJ61_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13545.1"
                     /translation="MAGKNIAARPASWRVLYVDSAGRNRVSTVKSQAEVDALVGELAG
                     QGITVGVKATAPAWRARFVDDAGKEHARHFPRKRDAQTWLDAQTTATVTGTYVVPNAG
                     KVTFKEYAESWRTSQVF"
     gene            complement(358..1053)
                     /locus_tag="PKJ61_06685"
     CDS             complement(358..1053)
                     /locus_tag="PKJ61_06685"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOC13546.1"
                     /translation="MRAPGLSPKSSRPDRDGGADVGPLGQTFAREVRRLRELRGLSQT
                     DLAAQLRERHALKFHQATIDRVEKGFRPCRLDEVYVLAEVLGSTVDDMLADWSSADSA
                     FRELTSIARRAGEAEYRIAMGIQQELAAIDGDRERVVAGLARSSDSDDGDQVQAWAEE
                     VLELLDALSAGAERILEGMTAHLPIRPGGVMEQVNRQYFGGILAAHEAAIAARRAEAA
                     AGDAQCLADGGPG"
     gene            1166..1360
                     /locus_tag="PKJ61_06690"
     CDS             1166..1360
                     /locus_tag="PKJ61_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOC13547.1"
                     /translation="MIDEAEIWFTTQVAEYLKTPATTIRWWRHIGQGPKSFKLGARKV
                     AYLRRDVEAWLEEQYTAHAV"
     gene            complement(2160..3341)
                     /locus_tag="PKJ61_06695"
     CDS             complement(2160..3341)
                     /locus_tag="PKJ61_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13548.1"
                     /translation="MLSRRGRRARTIATVLLSTLVFLASNAVAEAAPVDSSPVGESSD
                     ADRAAVAKELGISEEDALASLDFQGAVSAEALRLSTDPRVTSFWMESGRSPHLTIGVR
                     PGTATEPLRASLPAQLRRATKFTEQRFSEEELAQIGAALNQSLTGGSTIHVDVQHQKV
                     EVLATSDSGVEYAMTKVKAQRSADAWSDVVEVRVDRGLTAMPAVDIYGGRTSSGCTLG
                     FTVRNTSTGTRGTTTAAHCGNSQATAGINLPFKFEWLTGSSDVQWHTASSGTLRNWVT
                     DGTFDSTPYYRSITGTTARLSLVLGGTYCKFGATTGYGCGVLNSRTEPASWVPNSTPR
                     YLAIDSSTTQFCIGGDSGGPVYAGAKGVGLVSGCTGNQKRLISMSMDGFGGLPIVVAT
                     S"
     gene            3877..4275
                     /locus_tag="PKJ61_06700"
     CDS             3877..4275
                     /locus_tag="PKJ61_06700"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOC13549.1"
                     /translation="MTAFSSLPVREVVGEPGDGSGWPFTMAPVAQLVRDSLELSELTI
                     LVGENGVGKSTIIEAIAMAYGLSPEGGSKVTRASDRPSESPLHEHIRLVRGISRSRWG
                     YFVRAETMHGLLTYLEQNPSWHAPEPARSS"
     gene            4447..8034
                     /gene="mobF"
                     /locus_tag="PKJ61_06705"
     CDS             4447..8034
                     /gene="mobF"
                     /locus_tag="PKJ61_06705"
                     /inference="COORDINATES: protein motif:HMM:NF041492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobF family relaxase"
                     /protein_id="HOC13550.1"
                     /translation="MAITGMGIVRAAGAAGYVKYLSECGHSRPDEYFSEHGHAVVTWR
                     GIGADGQLVERQLTAEEFGRFMAGLDPETGASRWAQRVEPEHVAAVDFLVNGPKEWSI
                     LAVLDPGVQAALLRGQVNAERAVLRYLWENGTVQVKQAGRVVRVPVERIEIASVQHFS
                     SRAGDPHFHRHMEISARVWAAGKWRALDSLGARNLNVAVQAIFHREQLRELRPELARL
                     GYEVDEVGQIALLRPVVEAMSRRAAQVERNLAVIEAAWRVEHPGQEPTARILRKWGVL
                     AWEKQRPYKGEPGALDDELAVWAARIGVLMVEHGITEPVPGSAVDRRVSVAEFDRDAL
                     AAQLVETQGLRRSAWSRADLEAAAIMAVEGRVRGNRAAVDELAADVVARALGESRLLP
                     SVADGRPSTAVKWWTSPAVAERENELRAGLAVLADDGRLSVVEGAAGVGKTTSLAAYL
                     EEASVAGRRVAVVAPTGAAALVAAGAGVEASTVDSLLGRYGWDKNTAGSWVWAESHKR
                     PREPLPPGSRLVIDEAGMVSQDAARALVTLAAVCGWQVRVQGDSFQFSAVGVGGVMEI
                     AKGAAQPDRVALLEDASDVHRFRTADGGRDEAYAAHSIAIRSGTDPEATAARVMDRAT
                     VYASEAERQTAIAQEWAERAHAGTGLVIAGTNADVQQLNGLIADWLRAVGIVTGDTLA
                     TTPRGEWASETISEGDIVATRVNGVDEAGHRYANRERWRVDALDDAGAYLISLDGRRE
                     LRLSVGAAREQLQLAYAVTGYGAQGVTVNEAVLLVAEGMDRPSFYVGSTRGRYTNQAA
                     ILAPDGDANLARAQVEAILSQPGADTGWRAADLAAAADRQRMGDLVDMPSRHVPAIGG
                     GQVAEAAEQIARDADWPSEVRDPHLFDRALAYLDRLQRQRAVDAGQQEASLEQLRGTL
                     AKAIDANRQAALKVASATRGVGQAEKATKAAQEAVLAAEDRVRHANPFNRGSRERDLT
                     AARDTLWAARQAETGQRARRAELTQNAQRQQEASRRLSHEVELAETHLGWLRGERHQL
                     TLDLMPLPGVEYQQTYSRADLEALRDAWLAEPQPRDTADPIRAIIDLVNDDRHIADLA
                     TPSQWHTLADAASPDDLVWGGDYDKPWLRGMRQPLLGWLDQQANRLASDPQWHDAADQ
                     LTSRWTTIEQASETRRAELKPSPPADTPIARHREPPTLGPPGPDLTPGW"
     gene            complement(8465..9037)
                     /locus_tag="PKJ61_06710"
     CDS             complement(8465..9037)
                     /locus_tag="PKJ61_06710"
                     /inference="COORDINATES: protein motif:HMM:NF024861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HOC13551.1"
                     /translation="MARLQRVFIDTSELFPFTIMDVLLTLAEDFLFTWVWTDQILDEW
                     IDVIVREGKRTPESAASVAAAVRTHFGRYRIDPALYDDKITDDLSPDPDDRVHAAAAI
                     HGDVDVLLTRNLKHLRTKPVLAAGVKVSTSDDFLCELLAHRSQGVLESFIRAAESKTK
                     PPMTPHELADKISTAGAPRFAERLRPHLPR"
     gene            complement(9042..9419)
                     /locus_tag="PKJ61_06715"
     CDS             complement(9042..9419)
                     /locus_tag="PKJ61_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOC13552.1"
                     /translation="MTTHPLATLDPSDPTIRSDAKHLADRLSGDTPVEAAVRAMLDDV
                     AHGARVVVLRAEDEVTPAQAAAILGVTRQFVDRLCSDGVLAFRRLPGSRHRRIRVQDA
                     IDIAAERDQRRAGGDAIRAALVE"
     gene            complement(9871..10719)
                     /gene="map"
                     /locus_tag="PKJ61_06720"
     CDS             complement(9871..10719)
                     /gene="map"
                     /locus_tag="PKJ61_06720"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOC13553.1"
                     /translation="MTAIKPFPVSAQRAVPAHIERPHYVGRPRPERYTGAHVQTPETI
                     AKIREAGRIAVQAMHAGAAVIAPGVTTDEIDRVVHDFIVAHDAYPSTLGYRNFPKSCC
                     TSVNEVICHGIPDARPLEDGDIVKIDVTAFKDGVHGDNCATFFCGNVDETSRLLTERT
                     EEAMNRGIRAVRPGRQVNVIGRVIESYAKRFGYGVVRDYTGHGVHSSFHSGLVIPHYD
                     EPAMDTLIEVGMTFTIEPMLALGDYRSHTWDDKWTVVTNDCSRVAQFEQTIVVTPGGA
                     EILTAP"
     gene            10768..10968
                     /locus_tag="PKJ61_06725"
     CDS             10768..10968
                     /locus_tag="PKJ61_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002527760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13554.1"
                     /translation="MAEGEWYYCLDHHTVEPYQGCRSLSRLGPYASPADAAHALERVA
                     DRNEEWETDPRFNDPDEDEADD"
     gene            complement(11015..11404)
                     /locus_tag="PKJ61_06730"
     CDS             complement(11015..11404)
                     /locus_tag="PKJ61_06730"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOC13555.1"
                     /translation="MAAVFNAIGITVTDMAKTLTFYRLLGLEFEEADLAEGHVEWEGP
                     GGVRIMFDTLAVVHSFDPDWQPPVGGPAMSLAFECATPAEVDEAVAQVVQAGFDVHRE
                     PWDAFWGQRYATVLDPDGNRVDLFAWA"
     gene            11479..12816
                     /locus_tag="PKJ61_06735"
     CDS             11479..12816
                     /locus_tag="PKJ61_06735"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HOC13556.1"
                     /translation="MDKQTEFVLRTMEERDVRFVRLWFTDVLGFLKSVAIAPAELEGA
                     FSEGIGFDGSAIQGFARVYESDMVAHPDPSTFQLLPWRGSTQGTARMFCDIRQPDGAP
                     SFADPRYVLKRALKKASDMGFTYYTHPEIEFYLFKQPIVPGQPPEPADQSGYFDHTTT
                     SPGADFRRQAITLLEKMGISVEFSHHEAGPGQQEIDLRYADALTMADNIMTFRVVIKE
                     VAVMQNLFASFMPKPLSDHPGSGMHTHVSLFEGDTNAFYDATAEYHLSKTGRQFIAGL
                     LKHAGEISLITNPWVNSYKRLVAGAEAPSYICWGQNNRSALVRVPMYKPSKGTSARIE
                     LRSIDSAANPYLAYAIILAAGLNGIENELELPEGAEDDVWSLTDRQRQALGIRPLPRS
                     LGNAIDLMEDSQLVAETLGEHVFEFFLRNKKAEYDSYRGNVSEWELRNNLPSL"
     gene            complement(13070..14845)
                     /locus_tag="PKJ61_06740"
     CDS             complement(13070..14845)
                     /locus_tag="PKJ61_06740"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HOC13557.1"
                     /translation="MFKNKRLRVVLGSGAALAVVAAVGLTVATTSTAASSRWVTATAA
                     AGDVTQTYTATGTTTRKNTEEAAFSVSGTVKSVSVAVGDEVDAGDTLATLKKGPLQLA
                     LLEAETQVAAKKAALYAAKHPSSTSSGSSGGSSGSSGGSGSSSGSGSGTGTTTITIDP
                     TVLSEAASRINLAVVSEAQVCEPVFGSIVPEAEPTATATPSASASATETPSASASATP
                     VPTETASAAPTETATPDAPEGSDSPSAEASSESPSAEASASQLAAAIEAANPTTAELQ
                     ACANARAEVIAANTNLQTIVQQLVAPKPNAGAGSSRGAGSSASVKKTSSAGSSSSSSV
                     SAAKVASAEADLLEAEQNLQSAQDDLDAAVLVAPIAGKVGTVGLSVGSSASSGSITIV
                     GEGTAVVTLELPLKTRNLVTIGQAVTVAPAGSTTTLEGKITTISVLETSGTSGDSPTY
                     ATTVSVADPEMRLATGASASVAIRVLAVTGVLTVPASAVTPTGTGTASVLVLASANAE
                     TPETVEVKTGAVGAGRVEITEGLATGQLVVLADRTADIPSNTTNRRSRTSSTSSSSSG
                     ATASAQPSAAATTQPSSQPTASASR"
     gene            complement(14845..16077)
                     /locus_tag="PKJ61_06745"
     CDS             complement(14845..16077)
                     /locus_tag="PKJ61_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015069120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOC13558.1"
                     /translation="MMEWSETIRTAIAALNARRMRSLLTMLGILIGISAVMLTVGLGQ
                     GAQQQITAQINSLGSNMIIVSPSQTTSSGGFRGGGGAATTLTTQDAAMLADAEVAPDI
                     AAVAPTSSTSGSLQSSSTTWTSTVVGTVPDWQTVRARTVGSGRFFSQAEVDSAASVAV
                     IGSETATELFTTGSPVGRTFSINGQSFTVIGVLESAGSSVSSNDDDTVVVPLTTYASR
                     LSSSSAANAVSTIYLSGKDADSLSAAYQQVKTALLASHQVTSTDADFSVSTQASLVET
                     ATSITGVLTLLLGGIAAISLLVGGIGVMNIMLVSVSERVREIGLRKALGATPAVIRRQ
                     FLVEAAILGMLGGLVGIILGFTGAAILTPLIGMTVSISIPATVLALVVSVGIGLVAGV
                     YPATRAAKLAPIDALRSE"
     gene            complement(16074..16763)
                     /locus_tag="PKJ61_06750"
     CDS             complement(16074..16763)
                     /locus_tag="PKJ61_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOC13559.1"
                     /translation="MLNMTGVRKTYATGSVEVEALRGIDLHVEQGEYIAVMGPSGSGK
                     STLMHIIGCLDVPTSGTYTLDGVDVSAMTEKQLAEVRNQKIGFVFQQFNLLASLTALR
                     NVELPMIYAGVPAHERRERAMSALTKVGLADRIEHRPGELSGGQQQRVSVARALVTNP
                     TLILADEPTGNLDSHSTREILELFEELNDAGRTIVMITHELDVAERAKKMSHIYDGLL
                     IDEVPTHGAAK"
     gene            complement(16767..17963)
                     /locus_tag="PKJ61_06755"
     CDS             complement(16767..17963)
                     /locus_tag="PKJ61_06755"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HOC13560.1"
                     /translation="MTWKTWLIAGLAVALVAGAGVGTWMLLQPKATGNRGAFTQTAQA
                     TLGTQTQSVSLEGTLSPSKQSNVSFTVSGTVTSVKVKAGDVVEKGQKLATIDDSSLAD
                     AVDLAEANLTSAEANLDEVEDNDGSDAAINAASAQVDSAKAALTSAKEDLADAALRSP
                     IAGTVATVSVEVGDSVGSGSSSSSGSGGSSAGGTSSSSTSSSSSAQFVVIATSKWKLE
                     GSVGASDLGSIKAGQTAKVTVNGNEVDAEVASVGIVATSTSDGAATFPVVINLSGTHK
                     DLYSGTTASAVITTGSYDNVLTVPTAALSTNDGNTVVTLVDGSATSSVAVEVGRVFGD
                     YTEITSGLSDGDTVQITFRMPSSTSTSSSEEQGGFEFGGGLGGGLGGGQPPGGGQPPA
                     GGTGGR"
     gene            complement(18272..18652)
                     /locus_tag="PKJ61_06760"
     CDS             complement(18272..18652)
                     /locus_tag="PKJ61_06760"
                     /inference="COORDINATES: protein motif:HMM:NF036504.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterol carrier family protein"
                     /protein_id="HOC13561.1"
                     /translation="MRSKVSASQLQELRSINARLGDASAPLTRAELAASARTICALLG
                     RTHPGGSVELRVPPFAAVQLGIGERGRHTRGTPPNVVQTDAGTLLELATGRLSWTDAI
                     TARRVQASGIHSDLSELWPLAELR"
     gene            18677..21637
                     /locus_tag="PKJ61_06765"
     CDS             18677..21637
                     /locus_tag="PKJ61_06765"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamine synthetase]
                     adenylyltransferase/[glutamine
                     synthetase]-adenylyl-L-tyrosine phosphorylase"
                     /protein_id="HOC13562.1"
                     /translation="MTRNESLSGRLARLGFADAGRAAELVAEWAHWGSADQIELALVE
                     LPGCADPDLALTGFDRLLRARPDLLAELESHPGFTDRICAVLGSSVALNQHLGTHPDD
                     AEVLKGSIERRTPAELRRELLDAVGADADVSEPVADPSQSDALRRAYRRALLRIAARD
                     LCAPDPLVELPEVAGELADLADATVECALALARGEVPGWEQCRLGIIALGKTGARELN
                     YVSDVDVLYLAEPSIHSDGTPACDSGTAITIANRLASALSRVCSAHTAAGTIWPIDAA
                     LRPEGKAGPLVRTLASHRAYYEKWAKNWEFQAMLKARPMAGDLALAQEFVDMIWPMVW
                     QVADNDQFVGETQAMRKRVISLLPPKEAQVEIKLGAGGLRDVEFTVQLLQLVHGRSDE
                     RLRLRGTFEALTALVDHGYVGRGDGHDLGTAYRLQRLLEHRVQLYRLRRTHLMPADEA
                     NQRWLARSVGLSETKELMHLWRSTTRRVLTLHQRVFYSPLLETVARIPSAQVRLTPEA
                     AEIRLRALGYDDPKAALRHIQALSTGISRQSEIQRQLLPAMLTWFTAGPNPDHGLLAF
                     RQMSEALGSTPWYLRALRDGDAMAEHLARILASSRYAVDLLLRAPQNAALLGDPDGLR
                     PRPAAEVLTQMLTAARRHDSDVAAIAAVRASRRSELLRLAMADLLGQTDLATLGTALS
                     DLAGATIDAALEVAGRGVAAPPIAVIAMGRWGGRELAYASDADAMFVAADSDDPGATK
                     AAMTVISRLRSLLSRPGPDPALEIDIDLRPEGKGGALVRSLSSYRAYYARWSSTWEAQ
                     ALLRAGVGAGDRDLGAALLASIDAIRYPDAGLVRAQVLEIRKLKARMEAERLPRGGDP
                     SGHVKLGPGGLSDVEWVVQLLQLQHAAALPGLRTTNTLAALAVAVENELVTVDDAEAL
                     RSAWVLASRIRNAVMLLRGRVGDRLPTDSRELSAVAQVLGYAKGESSLLVEDYRRHAR
                     MARQVMDRLFWGAP"
     gene            21685..23118
                     /locus_tag="PKJ61_06770"
     CDS             21685..23118
                     /locus_tag="PKJ61_06770"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOC13563.1"
                     /translation="MSAIQNPDLPTTTASLSGWGRTAATTAQVLSTPDVELIAEAVAQ
                     VAGHNADSPSHLSRGVIARGLGRSYGDVAQNAGGLVVDMTPLDRIHSIDPDAALVDVD
                     AGVSLDALMRAALPYGLWVPVLPGTRQVTVGGAIGCDIHGKNHHSAGSFGDHVASLQL
                     LVADGRVLTLAPDGTPDDPDATLFWATIGGVGLTGIILRVVLKMTRTETAYFLADGVV
                     THTLDETIAVHSDGSEARYDYSSAWFDAISPEPKLGRAALSRGSLATLDQLREHAPKL
                     AADPLAFNAKPLFELPDVFPNGLANKFTFGLLGTAWYAKSGNYTDKIQSLTGFYHPLD
                     MFSEWNRAYGSHGFLQYQFVVPPAAIAEFKQLIRDIQASGHYSFLNVFKLFGEGNRAP
                     LSYPMSGWNVCVDFPIKAGLHEFLAGLDARVLEFGGRLYTAKDSRTTAATFHAMYPEV
                     DAWIATRRRIDPHGVFVSDLGRRLELV"
     gene            23180..23941
                     /locus_tag="PKJ61_06775"
     CDS             23180..23941
                     /locus_tag="PKJ61_06775"
                     /EC_number="1.1.1.333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenylphospho-beta-D-erythro-pentofuranosid-
                     2-ulose 2-reductase"
                     /protein_id="HOC13564.1"
                     /translation="MIDATGNPQTILLLGGTSEIGLAIVKAYLAKQPVRVVLGVQPGD
                     ELRDATVAELKAAGASEVDALDFDATAFDTHESVIDAAWKIADIDVAVVAFGLLGDAE
                     EVWQDQAKAVQIAQVNYTGAVSVGVLLGQRMKAQGYGQIIAMSSVAGELVRRSNFVYG
                     STKAGLDGFYRLLGEALAPFGPKVLVVRPGQVRTRMSADVKEAPLTVDKEQLATQVVD
                     AARNGAKVIWVPAPFQFVMMVLKHIPKPIFRLLPL"
BASE COUNT         3718 a         8028 c         8307 g         3939 t
ORIGIN      
        1 ggaagacctg cgaggtccgc cacgactctg cgtactcctt gaacgtcacc ttcccggcgt
       61 tgggcacgac atatgtcccg gtgaccgttg cggtcgtctg cgcatcgagc caggtctggg
      121 cgtcccgctt ccgcgggaag tgcctggcgt gctccttgcc ggcatcgtcg acgaaacgtg
      181 cccgccaggc gggcgcagtc gccttgactc cgaccgtgat tccttggccc gcgagttcgc
      241 cgacgagcgc gtcgacctcc gcctgggatt tcaccgtcga cacgcggttc cgcccggcgg
      301 agtcgacgta cagcactcgc cacgacgcgg gcctggcggc gatgttcttg cctgccatca
      361 gcccgggcct ccatccgcga ggcactgagc gtctccggcg gcggcctccg cgcgacgggc
      421 ggcgatggcc gcctcatgag cagccaggat ccccccgaag tactggcggt tcacctgctc
      481 catcacgcca cccggcctga tcggaaggtg cgccgtcatg ccttcgagaa tgcgctccgc
      541 gccggcggag agggcgtcga gtagttccag cacctcttcg gcccaggcct gtacctggtc
      601 gccgtcgtct gagtcgctgc ttcgggccag gccggccacg acgcgttcgc ggtcgccgtc
      661 gatcgctgcc agttcctgct gaatgcccat ggcgatccgg tattccgcct cgcctgcccg
      721 cctcgcgata ctggtcagct cacggaacgc actgtcggca ctcgaccagt ccgccagcat
      781 gtcgtccacc gtggacccca ggacctcggc aagtacgtac acctcgtcca gccggcaggg
      841 ccggaaaccc ttctcgaccc ggtcgatcgt cgcctggtgg aacttcaggg cgtgccgctc
      901 gcgcagctgc gccgccagat cggtttggga caaaccgcga agctcacgga ggcgccgtac
      961 ctcgcgcgcg aaggtctggc ccaggggacc gacgtctgca ccgccgtcac ggtcgggccg
     1021 actgctcttc ggggacaatc cgggtgcacg catacctgaa ccataacaac accatgttgc
     1081 gttgccaata tggtgcttga cagccgcgtc ttcgcaacgc acaatcagtg tgctgggatc
     1141 ccatcaccct gttatggagc gagcgatgat cgacgaagcc gagatctggt tcaccaccca
     1201 ggtcgccgag tacctcaaga cccccgccac gaccatccgg tggtggcgcc acatcggcca
     1261 gggccccaag agcttcaagc tcggcgcccg caaggtcgcc tacctcagac gcgacgtcga
     1321 agcctggctc gaggagcagt acaccgctca cgcggtctag ggacaggtcc tagcggggat
     1381 catcgacggc acgccgacct cggaccgcgt ccctgacgcg atttgcagcc gagtgggtcc
     1441 gtggcccggg ccgaaacgct cgatgcccag aggccggtcg tgatcgcgag catccaagcc
     1501 acaggcaggc gagggtgaag gccggacaga cggcccgacc tgcgattcca cgacttgcgc
     1561 cacacgggcg ccgtgctgga tgcccagacc ggcgcgaccc tcgccgaact catgggcagg
     1621 cgcggccacc cgagcgcgaa ctgctcagct tcgtggccgt catagccacc taacaactcg
     1681 acgacacggg cgaagacgct caggtatccg gacggacgag tgcccgatgg cggtcgctca
     1741 gctcgcctgg atcgtctgca ccaccgcgac ctcgacggtt ccggcaccta agcactggga
     1801 ggcgtgctgc tgtgaggcag gatcgaggag tccccacaca tctgcggcgg cgaggattcc
     1861 gcctccgccg gcgatccgag cgccggacgc tatcgtccgg ccatccggca gccgcacagt
     1921 ctgtcccgac aggacagatc cggcgggcca gacaaggggc tggccctcga gttggtagca
     1981 gccgctcggc gagagtgtca cggtcccctc gacgctcgct gccatctgat actccgtagc
     2041 ccgcacagtg agcaggatag gtggcggtgt gttggcagtg caggcaccca gagtgagcgc
     2101 accgatcagt gcggcggcga cgcgaagagc tcgaccgcga agaccgacca tctcgacctt
     2161 cacgatgtcg ccaccacgat gggaaggccg ccgaaaccat ccatcgacat ggaaatgagt
     2221 cgcttctggt tccctgtgca tccgctcacc aagccgactc cttttgcgcc tgcataaacg
     2281 ggaccaccgc tatcgccacc gatgcagaac tgagtcgtgc tgctatcgat tgccagataa
     2341 cgcggagttg agttcggcac ccatgacgct ggttcggttc tcgagttaag caccccgcag
     2401 ccatatccag tcgtggcacc gaacttgcag taggttccac ccagaacgag gctgagcctt
     2461 gcagttgtgc ccgtaatgct ccggtagtac ggcgtcgaat cgaaggtgcc atcggtcacc
     2521 caattgcgca aggtgccgga ggacgcggtg tgccactgca catcggacga tccggtaagc
     2581 cattcgaact tgaagggaag attgattcca gccgttgcct gactgttccc gcagtgagcc
     2641 gccgtggttg tccccctcgt ccctgtgctc gtgttgcgaa cggtgaaccc cagagtgcaa
     2701 ccgctgcttg tccggccgcc atagatgtcc acggctggca tcgcggtcag cccgcgatcc
     2761 acgcgaacct cgacaacatc gctccaggca tcggcgctcc gctgggcctt caccttggtc
     2821 atcgcgtact cgacaccgga gtcgctcgtg gcgaggacct ccaccttctg atgctgcaca
     2881 tcaacgtgga tcgtcgatcc tcccgtaagg ctctggttca gggctgcacc aatctgcgcg
     2941 agctcttcct ccgagaatcg ctgttcggtg aacttcgtcg cccgtcgcag ttgagcgggc
     3001 aaggaagccc gcagcggctc ggtggcggtc cccggccgaa cgccgatcgt caggtgaggt
     3061 gaccttccgg actccatcca gaaactggtg acacgaggat cggtcgacag gcgtaaggcc
     3121 tctgccgaga cggcgccctg gaagtccagc gacgcgaggg cgtcctcttc gctgatgcca
     3181 agctccttgg cgacggcggc tcgatcggcg tcgctcgact cgccgacggg acttgagtca
     3241 acgggcgctg cttctgccac ggcattggat gcgagaaata cgagcgtcga gagaagaacg
     3301 gtggcgattg tccgggcgcg acgaccgcgc cttgagagca tttgtcctcc aattgggggt
     3361 gaatgcggga agtgtatgtg gccgccggag cttcagtcaa tgagtcgtga actcacggag
     3421 tcgagtggcc tccaatattc cgacgctgac ctgacttggg gcacgtttcg tgacttgtgt
     3481 ggctgaatgc gggactggct aggtggttcg ctcggtggtg gtggtggtga cccgatctgt
     3541 tgtggttttg ggcaccgcaa gcggcagggc tctggtctcg acccgcgccg atgcggttac
     3601 ggcaggtgtt gggcgcctct tttccacaac aactcgggtg tgggccctga cctgccttgt
     3661 ccgggcgaca ataaccacaa caacgcgggt caatcggccc aatggcgggc atcaacaggc
     3721 aagccggcat ccctggcccg ccgaatccct gcccgaggtc tttcgcccgc caacccgttc
     3781 cgcattcggc aagatcgccc gcgcgcaccg ctgcatagat cggatgttcg gcttcgccgg
     3841 ttcggacgtg tctgcttcgc gtcctaccct gtcgtcatga ccgcgttcag ttcgctgccc
     3901 gtccgtgagg tggtcggtga accgggcgat ggcagcggct ggccgttcac aatggcaccc
     3961 gttgcccagc tggtgcgaga cagtctcgag ctgtccgagc tgaccatcct cgtcggggag
     4021 aacggggtcg ggaagtcgac catcatcgag gcgatcgcca tggcctacgg gctcagcccc
     4081 gagggtggca gcaaggtgac cagggcgtcg gaccgtccga gcgagtcgcc gctccacgaa
     4141 cacatccggc tggtgcgcgg catcagccgc tcccgctggg gttacttcgt gcgggctgag
     4201 accatgcacg gactgctcac ctacctagaa cagaacccgt cctggcatgc accggaaccc
     4261 gcccgatcct catgatcccg gacgtccgga gccggtgctc catccccgcc tgctccagtc
     4321 caccaacccc tacgccagca gtacctcgcc gcgtcccctc gaaccccaga cgctagtccc
     4381 agccgccccc aagacatcag acccgagcgg tcttgcttcg aagcgtacgc aatgtactat
     4441 tgggatgtgg ctatcaccgg tatgggtatc gtgcgggcgg cgggcgccgc cgggtacgtg
     4501 aagtacctgt ccgagtgcgg ccattcgcgc ccggacgagt acttctctga gcatgggcac
     4561 gcggtggtga cctggcgcgg tattggtgcc gacgggcagt tggtcgagcg gcagctgacg
     4621 gcggaggagt tcggcaggtt catggctggt ctggatccag agaccggcgc gtcccggtgg
     4681 gcgcagcgag tggagccgga gcacgtcgcg gcggtcgatt tcctagtgaa cgggccgaag
     4741 gagtggagca tcctcgccgt tctcgacccg ggcgtgcagg ccgcgctgtt gcgtggccag
     4801 gtgaacgccg agcgggccgt gctgcggtac ttgtgggaga acgggacggt gcaggtgaag
     4861 caagccggcc gggtggtgcg ggtgccggtg gagcggatcg agatcgcgtc ggtgcagcat
     4921 ttctcgtccc gggcagggga cccgcatttc caccggcaca tggagatctc ggcccgggtg
     4981 tgggcggcgg gcaagtggcg ggcgctggac tccttgggag cgagaaacct gaacgtggcc
     5041 gtgcaggcga tcttccaccg cgagcagctg cgcgagttga ggccggagct cgcccggctc
     5101 ggctatgagg tcgacgaggt cggtcagatc gcgctgctgc gaccggtggt ggaggcgatg
     5161 agccgacggg cggcgcaagt ggagcggaac ctggcggtga tcgaggccgc gtggcgggtg
     5221 gagcatccag ggcaggagcc gaccgcaagg attctgcgga agtggggcgt actggcgtgg
     5281 gagaagcaac gaccgtacaa gggcgagcct ggtgctttgg atgatgagct ggcggtgtgg
     5341 gctgctcgga tcggcgtgtt gatggtcgag cacgggatca ctgaacctgt cccggggagt
     5401 gctgtcgaca ggcgggtcag tgtggccgag ttcgaccggg acgcgctggc ggctcagttg
     5461 gtggagacgc agggtctgag gcggtcggcg tggtcgcggg cggatctgga ggcggcagcg
     5521 atcatggccg tcgaggggcg ggtgcggggg aatcgtgccg cggtcgacga gctggctgcg
     5581 gatgtggtgg cccgggcgtt gggggagtcc cggctgttgc cgtcggtcgc cgacggcagg
     5641 ccatcgacgg cagtgaagtg gtggaccagc cctgcggtcg cggagcggga gaacgagctg
     5701 cgcgccgggc tggccgtcct ggctgacgat ggccggttga gtgtggtcga gggcgccgcc
     5761 ggtgtgggga agacgaccag cttggcggcc tacctcgagg aggcatccgt ggcgggcagg
     5821 cgggtcgccg tggtcgctcc cacgggggcg gcggccctcg ttgctgcagg ggctggcgtg
     5881 gaggcgtcca cggtggactc gctgctggga cggtacggtt gggacaagaa cacggccggg
     5941 agctgggtct gggccgagtc ccacaaacgg ccgcgggagc cgctgccgcc cggtagcagg
     6001 ctcgtgatcg acgaagctgg catggtgtcc caggacgcgg cccgggcgct ggtcaccctc
     6061 gccgccgtgt gcggttggca ggtccgggtc cagggtgact cgttccagtt ctccgcggtc
     6121 ggcgtgggcg gtgtgatgga gatcgccaag ggcgccgcgc agcccgaccg ggtggccctg
     6181 ctggaggacg ccagcgacgt ccaccgcttc cggaccgcag acggcggccg ggatgaggcc
     6241 tatgcggcgc acagcatcgc gatccggagc ggaaccgacc ccgaagcgac cgccgcgcgg
     6301 gtgatggatc gcgcgacggt gtacgcctcc gaggccgaac gtcagacggc gatcgcgcag
     6361 gagtgggccg agcgtgctca tgccgggacg gggttggtga tcgccggcac gaatgcggac
     6421 gtgcagcagc tgaacgggct gatcgccgac tggttgcgcg cggtcgggat cgtgaccggt
     6481 gacacgctcg cgacgacacc gcgtggggag tgggcctcgg agacgatcag cgagggcgac
     6541 attgtcgcga cccgggtcaa cggcgtcgat gaggccggcc accggtatgc gaaccgggaa
     6601 cgctggcggg tcgacgctct ggacgatgcc ggggcgtacc tgatcagcct cgacggccgc
     6661 cgggagttgc ggctgtcggt cggcgcggcg cgcgagcagc tgcaactggc gtatgcggtg
     6721 accgggtacg gcgcccaagg ggtcacggtc aacgaggctg tcctgctggt cgccgagggc
     6781 atggaccgtc cgtcgttcta tgtgggctcg acccgcggcc gctacaccaa ccaggccgcg
     6841 atcctcgccc ccgatgggga cgcgaacctg gcgcgcgccc aggtagaggc gatcttgtcc
     6901 cagcctggcg ccgatacagg atggcgtgcc gccgacctgg ccgcggccgc cgaccggcaa
     6961 cggatgggcg acctcgtcga catgcccagc cgtcacgttc ccgcgatcgg tggcggacag
     7021 gtcgccgagg ctgccgagca gatcgcacgg gacgccgact ggccgagcga ggttcgcgat
     7081 ccccacctgt tcgatcgggc actcgcctac ctcgaccggc tccagcgcca gcgggcggtc
     7141 gacgccggcc agcaggaggc cagcctggag cagctccgag gcacgctcgc caaggccatc
     7201 gacgccaacc gacaggcagc gctcaaggtg gccagtgcga cgcgcggagt cgggcaggcc
     7261 gagaaggcca caaaggctgc ccaggaagcc gtgctggccg cggaggaccg tgtcaggcac
     7321 gcgaacccgt tcaaccgcgg cagccgcgag cgtgacctca ccgccgcccg cgacacgctc
     7381 tgggccgccc ggcaggccga gaccggccag cgtgcgcgtc gcgccgaact cacccagaac
     7441 gcccaacggc agcaggaagc ctcccgccgg ctctcccacg aggtcgaact cgccgaaacc
     7501 catctcggct ggctgcgcgg cgagcggcac caactcaccc tcgacctgat gccgctgccc
     7561 ggcgtggaat accagcagac ctacagtcgc gccgacctcg aagcgctccg ggacgcctgg
     7621 ctcgccgaac cccagccccg cgacacggcc gacccgatcc gggcgatcat cgacctcgtc
     7681 aacgacgacc gccacatcgc cgacctcgcc acccccagcc agtggcacac cctcgccgac
     7741 gcagccagcc ccgacgacct cgtgtggggc ggcgactacg acaagccctg gctacgaggc
     7801 atgcgccaac ccctcctcgg ctggctcgac cagcaagcca accggctcgc cagcgacccc
     7861 cagtggcacg acgccgccga ccagctcacc agccgctgga ccaccatcga gcaggcctcc
     7921 gagacccggc gcgccgaact caaacccagc ccgcccgccg acacaccgat cgcccgccac
     7981 cgagaacccc caacgctcgg cccacccggc cccgacctca ctcccggctg gtgagatgtc
     8041 gcggtcgacg agtctccgac ctcgatgcct acggatcgct cgtttcagag ccgacccagc
     8101 cctccagtgc ctgccggagc ccctgctctg ggcggatggg tccaggtaac gccgatcgtg
     8161 ggtgctgccg ccctggtggc cgccctgttg tggcgcagac cagagcggca cgcgctgact
     8221 tctcgtgcgc cgacgatggt gcgggttgtc ggcgccactg tgacggccgt ggtcctcgtg
     8281 ttcgcgctct ggtggcacaa ggagttcatc gccttgccgg ccggcagcat ctcctggtcg
     8341 cgcccccaag ctcccgttga aggacttccc cagctcctcg gagcactcgt cgttgcggcc
     8401 gcggtcaact cgctgttcga ggagctcatg tggcgtgccg cattcgctga catgccgggg
     8461 gcgatcagcg cgggaggtgt ggccggagac gttcggcgaa ccgcggcgca ccggcagtgg
     8521 agatcttgtc ggccagctca tggggagtca ttgggggctt cgtcttgctc tccgccgcgc
     8581 ggatgaacga ttcgagaacg ccttggctgc ggtgggcgag tagttcgcag aggaagtcgt
     8641 cggaggtgct gactttgacg ccggcggcca ggaccggttt ggtgcgcagg tgcttgaggt
     8701 tgcgggtgag cagcacgtcg acgtcgccgt ggatcgccgc ggcggcgtgc acccggtcgt
     8761 cggggtcggg tgacaggtcg tcggtgatct tgtcgtcgta cagggccggg tcgatgcggt
     8821 accggccgaa gtgggtgcgc accgcggccg ccacggaggc ggccgattcc ggggtgcgtt
     8881 tgccctcgcg gacgatgacg tcgatccact cgtcgaggat ctggtcggtc catacccagg
     8941 tgaacaggaa gtcctcggcg agggtgagca gcacgtccat gatcgtgaac gggaacagtt
     9001 cgctggtgtc gatgaagacc cgctgcagtc gcgccacccg gctactccac gagggcggcg
     9061 cggatcgcgt cgcctccggc gcggcgctgg tcacgttcgg cggcgatgtc gatggcgtcc
     9121 tggacccgga tgcggcggtg gcggctaccg ggcaagcgac ggaaggcgag cacgccgtcg
     9181 gagcagagtc ggtcgacgaa ctggcgggtt acgccgagga tggcggcggc ctgggcgggg
     9241 gtgacttcgt cctcggcgcg gagcaccacg acgcgggcac cgtgggcgac atcgtcgagc
     9301 atcgcgcgca cggcggcctc gaccggggtg tcgccgctga ggcggtcggc caggtgtttc
     9361 gcgtcggacc ggatggtggg gtcgctcggg tccagggtgg cgagcgggtg ggttgtcatg
     9421 tcctccatgg tacgcacaag tgcaataaca gcaacctcct acctcgacgc cgccgttgag
     9481 gaagcacgcg acgaacgcgg gagactgttg attccgactc cagggtgggc cagacggcac
     9541 cggaccgagt gcttgggaga gagcgaccgg aacacggatt tggtgtcggt cgcggggggc
     9601 ttgggagtcc gcagggagtc cgcaaaatct gcagctgccc caaacgccgc caacctgcca
     9661 aactgagaaa tgcctagtca ggtgcggttt gccaacctgg ccaacatgac caattccggc
     9721 cgtttaggcc gaaaacagaa tccggcttat cgaccgactc gtccccaacg cccgcgcaac
     9781 ttggccgcga ggtccaacgc tgggtggaac ctgagcagcg acccggctgc gtgaactgga
     9841 ccgcttcgaa ctgagttctc tttgccaggc tcagggagcg gtgaggatct cggctccgcc
     9901 cggcgttacc acgatggtct gttcgaactg ggccacgcgg ctgcagtcgt tggtgacgac
     9961 ggtccacttg tcgtcccagg tgtgcgagcg gtagtcgccc agcgccagca tcggttcgat
    10021 ggtgaaagtc atcccgacct cgatcagggt gtccatcgcg ggttcgtcgt agtgcgggat
    10081 caccagcccg gagtggaacg aggagtgcac gccgtggccg gtgtagtcgc gaaccacgcc
    10141 gtagccgaaa cgcttggcgt aggactcgat cactcgtccg atgacgttca cctgccggcc
    10201 cggccgcacg gcgcggatgc cgcgattcat ggcctcctcc gtccgctcgg tgaggagtcg
    10261 ggacgtttcg tcgacattgc cgcagaagaa ggtcgcgcag ttgtcgccgt gcacaccgtc
    10321 cttgaacgcg gtgacgtcga tcttcacgat gtcgccgtcc tcaagcggac gcgcgtccgg
    10381 gatcccgtgg cagatcacct cgttcaccga agtgcagcag gacttgggga agttgcgata
    10441 gcccagcgtg gacgggtagg cgtcgtgcgc gacgatgaag tcgtgcacca cccggtcgat
    10501 ttcgtcggtc gtgacgccgg gggcgatcac cgcagcgccg gcgtgcatgg cctgcacggc
    10561 gattcgtccg gcctcccgga tcttcgcgat cgtctccggg gtctgcacgt gtgcgccggt
    10621 gtaccgctcc ggacggggtc gcccgacata gtgcggacgc tcgatgtgcg cgggcaccgc
    10681 tcgctgggcc gacacaggaa aaggcttgat cgcggtcacg aacgtcgatc atagtggggc
    10741 gatagccgcg cactgctggg aggagccatg gctgagggcg agtggtacta ctgcctcgac
    10801 catcacaccg tcgagcccta ccagggctgc cgtagtctga gccggctggg cccctacgcc
    10861 tcgccagcgg acgcggccca cgccctcgaa cgcgtggccg accgaaacga ggagtgggag
    10921 accgatcccc ggttcaacga tccggacgag gacgaggcgg acgactgact cgccaggaac
    10981 gcggtctcga ccggctcgac cagttgagcg ggcgtcaggc ccaggcgaac aggtcgacgc
    11041 gattcccgtc cggatcgagc acggtcgcgt agcgctggcc ccagaacgcg tcccagggct
    11101 cgcggtgcac gtcgaagccg gcctgcacga cctgggcgac ggcctcgtcc acctcggcgg
    11161 gcgtcgcgca ttcgaaggcc agggacatgg ccgggccgcc caccggcggc tgccaatcgg
    11221 ggtcgaagct gtgcaccacg gcgagtgtgt cgaacatgat ccggacgccg cccggaccct
    11281 cccactcgac gtggccctca gccaggtccg cttcctcgaa ctcgagcccg agcagccggt
    11341 agaacgtcag ggtcttggcc atatcggtga cggtgatgcc gatggcgttg aagacagcag
    11401 ccatgccttc aggatacgga cgcggccacg ccggtgatgt aaccccactg aaacactgcg
    11461 ggcggcagac ttggcggcat ggataagcag accgagttcg tgcttcgcac catggaggag
    11521 cgcgacgtcc gtttcgtccg gctgtggttc accgacgtgc tgggtttcct gaagtccgtc
    11581 gccatcgccc cagccgagct cgaaggcgcc ttcagcgagg gcatcggctt cgacgggtcg
    11641 gctatccagg gcttcgcgcg ggtctacgag tcggacatgg ttgcacaccc cgatccgtcc
    11701 accttccagt tgctgccctg gcgcggatcc acccagggca ccgcccggat gttctgcgac
    11761 attcggcagc cggacggggc accgtcgttc gccgacccgc gctacgtgct gaagcgggcg
    11821 ctgaagaagg cttccgacat ggggttcacc tactacacgc accccgagat cgagttctac
    11881 ctgttcaagc agccgatcgt gccgggccag ccgcccgagc cggctgatca gtcgggctac
    11941 ttcgaccaca ccaccacctc gcccggcgcg gacttccgcc ggcaggcgat caccctgctg
    12001 gagaagatgg gcatttcggt cgagttcagc caccacgaag ccggtccggg gcagcaggag
    12061 atcgacctgc gttacgcgga cgccctgacg atggccgaca acatcatgac cttccgggtg
    12121 gtgatcaagg aggtcgcggt gatgcagaac ctgttcgcgt ccttcatgcc gaagccgctg
    12181 tcggaccacc ccggctcggg catgcacacc cacgtctccc tgtttgaggg cgacacgaat
    12241 gccttctacg acgccaccgc ggagtaccac ctgtccaaga ccggacgcca gttcattgcg
    12301 ggcctgctca agcacgccgg ggagatctcg ctgatcacca acccgtgggt gaactcctac
    12361 aagcggctgg tggccggcgc ggaagcgccg agctacatct gctgggggca gaacaaccgg
    12421 tcggcgctgg tccgcgtccc gatgtacaag ccgagcaagg gcacgtcggc gcggatcgaa
    12481 ctgcgctcga tcgattccgc ggccaacccc tacctggcgt acgcgatcat tctcgccgcc
    12541 gggctgaacg gcatcgagaa cgagcttgag ctgcccgagg gcgcagagga cgatgtctgg
    12601 tcgctgaccg accggcagcg gcaggcgctg ggcattcgcc cactgccgcg cagccttggc
    12661 aacgccatcg acctgatgga ggattcccag ctggtggccg aaaccctggg cgagcacgtt
    12721 ttcgagttct tcctgcgcaa caagaaggcg gagtacgact cctaccgcgg aaacgtctcg
    12781 gagtgggagc ttcgcaacaa cctgccgtcg ctctgagttc ttgccgtccg cgctggcccc
    12841 ggacggcgct cgcgaccctg agtggcgcga aaagtgtacg gacctgcacg aggtggcttt
    12901 tgtttagcgc tggcctcgcc ttcaatggat tcagccgtcg tgcccttagg ggccgtcacg
    12961 cccgcgttgg tgcccagggg aggggtggta ccgatccgcg tggacggcga cgagcccgcc
    13021 gaggtcaatc tgggagatga cctcgacggg ctcgatggcg tccggacgct cagcggctgg
    13081 cgctggccgt cggctgcgaa cttggctggg tagtggctgc ggcgctcggc tgagcagacg
    13141 cggtcgcacc cgatgaactc gacgaggttg aactcgtccg gctacgccgg ttcgtggtgt
    13201 tggacggaat gtccgcagtg cggtcggcca gcaccacgag ctggccggtg gccagtccct
    13261 cggtgatctc cacccgtccg gcgccgaccg ctccggtctt gacctcgacc gtctccgggg
    13321 tttcggcgtt ggccgaggcc agcaccagca cgctggcggt tccggtcccg gtcggcgtga
    13381 ctgccgacgc cgggacggtg agcacgccgg tgacggccaa cacccggatc gccacggacg
    13441 cgctcgcccc ggtggccagc cgcatttccg gatcggcgac cgaaaccgtg gtggcatacg
    13501 tgggggagtc cccgctggtg ccgctggtct ctagcacgct gatcgtggtg atcttgcctt
    13561 cgagcgttgt ggtcgagccg gccggtgcga ccgtcaccgc ttgtccgatg gtgaccaggt
    13621 tgcgggtctt cagcggcagt tcgagggtga ccacggccgt cccttcgccc acgatggtga
    13681 tcgaaccgga cgacgccgac gaacccacgc tgaggccgac ggtgcccact ttgcccgcga
    13741 tcggtgccac cagcacggca gcgtcgaggt cgtcctgcgc agactggagg ttctgctcgg
    13801 cttccagcag gtcggcctca gccgaggcaa ccttggccgc actcaccgaa ctggacgaac
    13861 ttgagccggc cgaggaggtc ttcttcaccg aggcggacga acctgcgccc ctgctcgagc
    13921 ctgcgcccgc gtttggcttc ggggcgacca gctgctgcac gatggtctgc agattggtgt
    13981 tcgccgcgat cacctcggca cgcgcgttgg cgcaggcctg aagctcagcc gtggtcgggt
    14041 tcgcggcctc gatcgcggca gccagctgcg acgcggacgc ttccgcactc gggctctcgc
    14101 tggaggcctc ggccgaggga gaatccgagc cctccggtgc gtccggtgtg gccgtctcgg
    14161 tcggcgcggc gctggcggtc tcggtgggga ctggcgtggc gctggcggac gcgctgggcg
    14221 tctcggtcgc cgaagccgac gcggacgggg tggccgtggc ggtcggttcg gcctccggca
    14281 cgatcgaccc gaacacgggc tcgcacacct gggcttcgct gacgacggcg agattgatcc
    14341 gcgacgccgc ctcgctcagc acggtgggat cgatggtgat cgtggtggtc ccggtcccgc
    14401 tgcctgagcc gctcgagctg cccgaaccac cggacgaacc gctagaccca cccgaactgc
    14461 ccgaggaggt gctggacggg tgcttggccg cgtagagcgc agccttcttc gcggccacct
    14521 gggtttccgc ttccagcagc gcgagctgca gcggcccctt tttcaaggtg gccagtgtgt
    14581 cgccggcatc gacctcgtcg ccgaccgcga cgctgacgct cttcacggtg ccgctgacgc
    14641 tgaaggcagc ctcctcggtg ttcttcctgg tggtggtgcc ggtggcggtg taggtctggg
    14701 tgacgtcgcc agctgccgcc gtagcggtca cccagcggct gctggccgcg gtcgaagtgg
    14761 tcgcgacggt caggccgacg gcggcgacga cggccagggc cgcgccactg cccagcacga
    14821 cgcgtaggcg tttgttcttg aacatcattc gctcctcagc gcatcgatcg gcgccagctt
    14881 ggccgccctg gtcgcggggt agacgccggc caccaggccg atgcccaccg agacgaccag
    14941 cgcgagcaca gtggctggga tggagatcga cacggtcatg ccgatcagcg gggtcaggat
    15001 ggccgccccg gtgaagccga ggatgatccc gacgaggccg ccgagcatgc ccaggatcgc
    15061 cgcctccacc aggaactgcc ggcggatcac tgcaggcgtg gcgcccagcg ccttgcgcag
    15121 gccgatctca cggacgcgtt cgctcaccga gaccagcatg atgttcatca cgccgatgcc
    15181 gccgaccagc agcgagatcg ccgcgatgcc gccgagcagc agggtgagca caccggtgat
    15241 cgaggtggcg gtctcgacca gcgaggcctg ggtgctgacg ctgaagtcgg cgtcggtgga
    15301 agtcacctgg tgcgaggcca gcagcgcggt cttcacctgc tggtaggcag cggaaagact
    15361 gtcggcgtcc tttccggaga ggtagatggt cgacaccgcg ttggctgcgc tcgagctgga
    15421 cagcctggac gcgtaggtgg tcagcggcac cacgaccgtg tcgtcgtcat tgctgctgac
    15481 cgaggacccg gccgattcca gcaccccgat caccgtgaac gactgcccgt tgatgctgaa
    15541 ggtccgcccg accggcgagc cggtggtgaa cagttcggtc gcggtctccg agccgatcac
    15601 cgcgactgac gcggcggagt cgacctcggc ctggctgaag aagcgtccgc tgccaaccgt
    15661 ccgcgcccgc acggtctgcc agtcgggcac cgttccgacc acggtcgacg tccaggtcgt
    15721 cgagctcgac tgcagcgagc cggacgtgct cgacgtcggc gccacggcag cgatgtcggg
    15781 ggcgacctcg gcatcggcga gcatggccgc gtcctgggtg gtcaacgtgg tggccgcccc
    15841 gccgcccccg cggaagccgc cggaactggt ggtctggctg gggctgacga tgatcatgtt
    15901 cgagccgagc gagttgatct gcgcggtgat ctgctgctgg gcgccctggc cgaggccgac
    15961 ggtcagcatc accgctgaga tgccgatcag gatgcccagc atggtcagca gcgaccgcat
    16021 ccgccgggcg ttcagcgcgg cgatcgcggt gcgaatggtc tcgctccact ccatcatttg
    16081 gcagctccgt gggtcgggac ctcgtcgatc agcaggccgt cgtagatgtg ggacatcttc
    16141 ttggcgcgct cggccacgtc cagctcgtgg gtgatcatca cgatcgtccg gccggcgtca
    16201 ttgagctcct cgaacagttc gaggatctcg cgggtggagt gcgagtcgag gttgccggtt
    16261 ggctcgtcgg cgaggatcaa cgtcgggttg gtgaccagcg ctctggccac cgacacgcgc
    16321 tgctgctggc cgccggacag ctcgccggga cggtgttcga tgcgatcggc aaggccgacc
    16381 ttcgtcagcg cggacatggc gcgctcgcgc cgctcgtggg cgggcacacc ggcgtagatc
    16441 atcggcagct ccacgtttcg cagcgcggtc aggctggcca gcaggttgaa ctgctggaac
    16501 acgaagccga tcttctggtt gcgtacctcg gcgagctgct tctcggtcat cgcgctcacg
    16561 tccacgccgt cgagggtgta ggtgccagac gtgggtacgt ccaggcagcc gatgatgtgc
    16621 atcagggtgg acttgcccga cccggacggt cccatcacgg cgatgtactc accctgctcg
    16681 acgtgcaggt cgataccgcg cagcgcctcc acttctaccg agccggtagc gtaggtcttg
    16741 cgaaccccag tcatgttgag cattggtcag cggcctcccg tgccgccggc cggaggctgc
    16801 ccaccgccag ggggctggcc gccgccgaga ccaccgccca gtccgccacc gaactcgaag
    16861 ccgccctgct cctcactcga ggaggtcgac gtcgacgagg gcatccggaa cgtgatctgg
    16921 acggtgtcgc cgtcgctgag gccgctggtg atctcggtgt agtcgccgaa tacgcgtccg
    16981 acttcgaccg cgacgctgct cgtggcggat ccgtcgacca aggtgaccac ggtgttgccg
    17041 tcgttcgtgc tgagcgctgc ggtgggcacg gtgagcacgt tgtcgtagct gccagtggtg
    17101 atcaccgcgg acgcggtagt cccggagtag aggtccttgt gggtgccgga caggttgatt
    17161 actactggga acgtcgcggc gccgtcgctg gttgacgtgg cgacgatgcc gaccgaagcg
    17221 acctcggcgt ccacctcgtt gccgttgacc gtcactttcg cggtctggcc ggccttgatg
    17281 ctgcccaggt cggacgcgcc gaccgagcct tcgagcttcc atttcgaggt ggcgatcacg
    17341 acgaactgtg ctgagctgga agagctggtg gacgagctgc tggtgccgcc ggccgacgag
    17401 ccgccggagc cgctgctgga cgatgatccg ctgcccacgc tgtcgccgac ctcgacgctg
    17461 accgtggcaa cggtcccggc gatgggggag cgcagcgcgg cgtccgccaa gtcctccttc
    17521 gcgctggtta gcgcggcctt cgcggagtcc acttgggcgg acgcggcgtt gatcgcggcg
    17581 tcgctgccgt cgttgtcctc cacctcgtcg aggttggcct cggcggaggt gaggttcgcc
    17641 tcggctaggt cgaccgcgtc ggccagcgag ctgtcgtcga ttgtggccag cttctgcccc
    17701 ttctcgacca cgtcaccggc cttgaccttc accgaggtca cggtgccgga cacagtgaag
    17761 ctcacattgg actgtttgct cgggctgagc gtgccctcaa ggctgacgga ctgggtctgt
    17821 gtgcccagcg tggcctgcgc ggtttgggtg aacgcgcccc ggttgcccgt cgccttgggt
    17881 tgcagcaaca tccaggtgcc cacaccagcg ccggcgacca gcgcgaccgc taggcctgcg
    17941 atcagccagg tcttccaggt catgcgaaac cggcgcttcg gcttgacggg cggtgggatg
    18001 gattcagtcc tatcggttgc cattccagcg ttctccttcg ggcaccttgc ccacgcatcg
    18061 aacgctatgg aagtgacctg tgccgagtcc tgtgcgaacc tgtgtccgag ctgtgcgcac
    18121 gcgtgttctt ggtgttcagc ggtcggcttc cccggctcag ccttcccggc caggacatgg
    18181 gacaaacctg ccacccatcc gtggtggtgg acggataccg gccagattct gcaactggcg
    18241 gcagctttgt cccgtttcgg agcgccgccg gtcagcgcaa ctcggccagc ggccacagtt
    18301 cgctgagatc gctgtgaatc ccggacgcct gaacgcggcg cgcggtgatc gcgtccgtcc
    18361 acgacaggcg gccagtcgcc agttccagca gcgtgcccgc gtcggtctgc accacattcg
    18421 gcggggtacc ccgggtatgc cggccgcgct caccgatacc gagctgcacc gcggcgaacg
    18481 gtggcacccg cagctccacg ctgccgcccg ggtgcgtgcg gccgagcagc gcgcagatgg
    18541 tccgggccga agcggccagt tcggccctcg tcagcggagc cgaggcgtcg cccagtctgg
    18601 cattgatgct tcgcagctcc tgcagctggc tcgccgacac cttgcttcgc accttccaga
    18661 cgctagcatc gcgctcgtga cccgcaacga gagcttgtcc gggcgtctgg cgcgactggg
    18721 tttcgccgat gccggcaggg ctgcggagtt ggttgctgaa tgggcccact ggggcagcgc
    18781 cgaccagatc gaactggcgc tggtcgagct accgggttgc gccgacccgg atcttgccct
    18841 caccggtttc gaccgtctcc tgcgcgcgcg gccggacctg ctcgccgagc tcgagtcgca
    18901 ccccggtttc acggaccgga tctgtgctgt gttgggttcg agcgtcgcgc tgaaccagca
    18961 tctcggcacg catcccgacg acgcggaggt gctcaagggc agcatcgagc gccgcacccc
    19021 cgctgagctt cgccgtgagt tgctcgacgc cgtcggcgcg gacgcggacg tctccgagcc
    19081 ggtcgccgat cccagccaat cggacgcgct gcgtcgtgcc taccgacgag ccctgctgcg
    19141 catcgccgcc cgcgatctgt gcgcgccgga cccgctggtc gagttgcccg aagtggctgg
    19201 ggagctggcc gatctggccg atgctacggt cgaatgcgcg ctggccctgg cccgcggcga
    19261 agtccccggc tgggagcagt gccgcctggg catcatcgcc ctgggcaaga ccggtgcccg
    19321 cgagctcaac tatgtcagcg acgtcgatgt gctctacctg gccgagccgt cgattcactc
    19381 cgacggcacc ccggcctgcg actccggcac cgccatcacg atcgcgaacc gcctggccag
    19441 cgcgctgtcc cgggtttgct ctgcgcatac cgccgccggc acgatctggc cgatcgacgc
    19501 tgccctgcgt cccgagggca aggccgggcc gttggtgcgg acgctggcgt cccatcgcgc
    19561 gtactacgag aagtgggcca agaactggga gttccaggcc atgctcaagg cccggccgat
    19621 ggccggtgac ctggcgctcg cgcaggagtt cgtcgacatg atctggccga tggtctggca
    19681 ggtcgcggac aacgaccagt tcgttggtga gacccaggcg atgcgcaagc gggtgatctc
    19741 gctgctgccg ccgaaagagg cacaggtgga gatcaagctg ggcgcgggcg gtcttcgcga
    19801 cgtcgagttc accgtgcagt tgctgcagtt ggtgcacggc cggtcggacg agcggctgcg
    19861 gctgcgcggc accttcgagg cgctgaccgc actggtcgac cacggctatg tcggtcgcgg
    19921 ggacggccat gacctcggca ctgcctaccg cttgcagcgc ctgctggagc atcgggtgca
    19981 gctgtaccgg ttgcgccgca cccatctgat gccggccgac gaggcgaatc agcgctggct
    20041 ggcgcgcagc gtggggctca gtgagaccaa agagctgatg catctttggc gctcgaccac
    20101 gcgccgcgtg ctgaccctgc accagcgcgt gttctactcg ccgctactgg agaccgttgc
    20161 gcggatcccg tccgcccagg tgcggctcac cccggaggcc gccgagatcc gcctgcgcgc
    20221 actcggctac gacgatccga aggctgcgct gcgccatatc caggcgctca gcaccggcat
    20281 ctcgcggcag tcggagattc agcggcagct cctcccagcg atgctcacct ggttcaccgc
    20341 agggccgaat cctgaccacg gcctgctggc gttccggcag atgtcggaag cccttggtag
    20401 tacaccgtgg taccttcgcg cgctccggga cggggacgcg atggccgagc acctggcccg
    20461 cattctggcc tcgtcgcgct acgcggtgga cctgctgttg cgcgccccgc agaatgcggc
    20521 gctgctcggc gacccggacg ggctgcgtcc gcgtccggcg gccgaggtgc tcacccagat
    20581 gctgactgcg gcgcgccggc atgattcgga cgtggccgcg atcgcggccg tccgcgcgtc
    20641 ccggcgaagc gaactgctgc ggctggccat ggctgacctg ctcggccaga ctgatctcgc
    20701 cacgctcggg acggccctct ccgacctggc gggcgcaact atcgacgcag cgctcgaggt
    20761 ggccggacgc ggggtagccg ctccgccgat cgccgtgatc gcgatgggcc gctggggtgg
    20821 acgcgaactg gcctacgcct cggatgccga tgcgatgttc gtggccgcgg acagtgacga
    20881 tccgggggcg accaaggcgg cgatgacggt gatcagccgg ctgcgctcgc tactgtcgcg
    20941 gcccgggccc gacccggcgc tggagatcga catcgacctg cggccggaag gcaagggcgg
    21001 cgcgttggtc cgcagcctca gctcgtatcg cgcgtactac gccagatggt cgtccacctg
    21061 ggaggcgcag gcgctgctgc gggccggggt gggtgccggc gaccgcgacc tgggcgcggc
    21121 actgctggct tcgatcgatg cgattcgcta ccccgatgcc gggctggtca gggcccaggt
    21181 gctggagatc cgcaagctca aggcgcgcat ggaggccgag cggttgcccc gcggcggtga
    21241 tccgtccggg catgtgaagc tgggcccggg gggtctctcc gatgtggagt gggtcgtgca
    21301 gctgttgcag ttgcagcacg cagccgcctt gcccggcctg cgcaccacaa acaccctggc
    21361 ggcgctcgcg gtcgcggtcg agaacgagct ggtgacggtt gacgacgccg aggcgctgcg
    21421 ttctgcctgg gtgctcgcgt cccggattcg caacgcggtc atgctgctgc gcggccgcgt
    21481 cggcgaccgg ctgccgacgg actcccgcga gttgtcggct gtcgcccagg tgctcggtta
    21541 cgcgaagggc gagtcgtcgc tgctggtcga ggactaccgg cggcacgcgc ggatggcccg
    21601 gcaggtgatg gaccggctct tctggggagc gccctgagcc cagcgtgccg acctgaccgg
    21661 acggcgcgag taggctgagg ctcgatgagc gccatccaga acccagatct gcccacgacg
    21721 accgccagcc tgtctggctg gggccgtacc gccgccacga ccgcccaggt cctgtcgacg
    21781 cccgatgtcg aactgatcgc cgaggcggtc gcccaggtcg ccggccacaa cgccgactcg
    21841 ccctcgcacc tgtcgcgagg cgtgatcgcc cgcggcctcg gccgctccta cggcgacgtg
    21901 gcgcagaacg ccggtgggct ggtggtcgac atgaccccgc tcgaccgcat ccactccatc
    21961 gacccggacg cagccctggt cgacgtggac gccggggtca gcctggacgc gctgatgcgc
    22021 gccgcgctgc cgtacggcct gtgggtgccg gtgttgcccg gaacccgcca ggtgaccgtc
    22081 ggcggtgcca tcggctgcga catccacggc aagaaccacc acagcgcggg atcgttcggc
    22141 gaccacgtcg cttccctgca gctgctggtg gccgacggcc gcgtcctcac cctcgccccg
    22201 gacggtacgc ccgacgaccc ggacgcgacc ctgttctggg ccaccatcgg cggcgtcggc
    22261 ctgaccggca tcatcctgcg ggtggtgctg aagatgaccc gcaccgagac cgcctatttc
    22321 ctcgccgacg gtgtggtcac gcacaccctg gacgagacga tcgcggtcca ctccgacggc
    22381 tccgaggcgc gctacgacta ctccagcgcc tggttcgacg cgatcagccc cgagccgaag
    22441 ctcggccggg cggccttgtc gcggggctcg ctggccaccc tcgaccagtt gcgtgagcac
    22501 gcgccgaagc tggcggccga cccgctggcc ttcaacgcca agccgctgtt cgagctgccg
    22561 gacgtgttcc cgaacggctt ggcgaacaag ttcacgttcg gcctgctggg caccgcctgg
    22621 tacgcgaaga gcggcaacta caccgacaag atccagagcc tcaccggctt ttaccacccg
    22681 ctggacatgt tcagcgagtg gaaccgcgcc tacggctcgc acggcttcct gcagtaccag
    22741 ttcgtggtgc cgccggctgc gatcgccgag ttcaagcagc tgattcgaga catccaggcg
    22801 tccgggcact actcgttcct gaacgtgttc aagctgttcg gcgaaggcaa ccgggccccg
    22861 ctcagctacc cgatgtccgg ctggaacgtc tgcgtggact tcccgatcaa ggcaggtctg
    22921 cacgagttcc tggccgggct ggacgcgcgc gtgctcgagt tcggcgggcg gctgtacacg
    22981 gccaaggact cgcgcaccac cgctgctacc ttccatgcga tgtacccgga agtggacgcg
    23041 tggatcgcca cccggcgccg gatcgatccg cacggggtct tcgtgtctga tctcggccgc
    23101 cggctcgaac tcgtctgaat cggacgccag aacggtccct gagcctgacg aagggccacc
    23161 accacaagga gttaccaaag tgatcgatgc caccggcaac ccccagacca tcctgctgct
    23221 cgggggcacg tccgagatcg ggctggccat cgtcaaggcg tacctggcca agcagcccgt
    23281 ccgggtggtg ctgggcgtgc agcccggcga tgagctgcgg gacgcaaccg tcgccgagct
    23341 gaaggcagcg ggcgcgtcgg aggtcgacgc tctcgacttc gacgccaccg ctttcgacac
    23401 ccacgagagc gtgatcgacg cggcctggaa gatcgccgat atcgacgtgg ccgtggtcgc
    23461 gttcggcctc ctcggcgatg ccgaagaggt gtggcaggac caggccaagg ccgtccagat
    23521 cgctcaggtg aactacaccg gcgcggtgag cgtcggcgtc ctgctcgggc agcgaatgaa
    23581 ggcgcagggc tacggccaga tcatcgcgat gagctcggtc gccggcgaac tggtgcggcg
    23641 atcgaacttc gtctacggct ccaccaaggc tgggttggac ggcttctacc gactgctcgg
    23701 cgaggcgctc gcgccgtttg gcccgaaggt gctggtggtg cgtccgggcc aggtgcggac
    23761 gcggatgtct gccgacgtga aggaagcacc gctcacggtg gacaaggagc agctcgccac
    23821 ccaggtggtg gacgcggccc gcaacggcgc caaggtcatc tgggtgccgg cccccttcca
    23881 gttcgtgatg atggtgctca agcacatccc gaagccgatc ttccggctgc tcccgctcta
    23941 ggcttctcag ctggtcgcga aggagctcac cgagcttccg cacacccagc tc
//
LOCUS       DAOPRQ010000040        22997 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_1842_length_22997_cov_11.588179, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000040 DAOPRQ010000000
VERSION     DAOPRQ010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 22997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22997
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1842_length_22997_cov_11.588179"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1185
                     /locus_tag="PKJ61_06780"
     CDS             <1..1185
                     /locus_tag="PKJ61_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020579005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecQ"
                     /protein_id="HOC13565.1"
                     /translation="LGMGFDKPDLGFVIHLGAPSSPVAYYQQVGRAGRAVASADVLLL
                     PTPADREIWHYFATASMPDEAKASAVIEALASAGSPMSTAALEAVVDIRRARLELLLK
                     VLDVDGAVTRVRGGWAATGAPWVYDRERYARVAAAREREQQLMLDYVDTSGCRMAFLQ
                     QTLDDPEPTRCGRCDRCAETWYADSVSSAAAESARAELGRAGVELVPRTLWPSGMAKL
                     GVPVSGRIGASDAAEPGRVLARFTDLGLGQRLRNLLAEPDAPVPDWVLPYCFEVLREW
                     DWAERPTAVLAVPSLTHPQLVATLAAELARSGRLTDLGSLALRSTEMEERAGNSAFRL
                     KQIFSRFTVTPDQADALVEAGPVLLVDDLVDSRWTITVAASLLRQAGATSVLPFALAA
                     VA"
     gene            1472..2791
                     /locus_tag="PKJ61_06785"
     CDS             1472..2791
                     /locus_tag="PKJ61_06785"
                     /inference="COORDINATES: protein motif:HMM:NF014741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HOC13566.1"
                     /translation="MASTVDEDLSALLDAYIATALVADPPPELPLWQYTERELLAELK
                     RVQQRSDAVAARQLALLAEAERRESTVHQASLPTAGWLVDHNTHSARSAREEVQFAVA
                     LAQSPSVGRALERGQVSVEQAKAIVNGLRRLPEDLDDSRRQLVAGQLVRFAADFGPAG
                     LARLVNRAIEVVAPEVAEDADRRSVERLDAEQRRDRYFTWRQNPDGSWTFHGKLPSVP
                     GQQLINVVRAVAKPHRSSVLLAGGQITGSQANADALCGLVEHYASCGIAPDHGADRPR
                     VIVTIDFDTLRGKLGTATLLDADCRITAQEARRIACDAHILPAVLGSKSKPLDVGRSK
                     RLFTKELRHLLILRDRGCTFPGCDRPPSECEAHHRKPWWAGGETSLDSGVLLCTFHHH
                     TVEPNPNLPIESQWLIRLDDRGLSEFAAPLRPNQAERVWKQHHRFRS"
     gene            complement(3145..4503)
                     /locus_tag="PKJ61_06790"
     CDS             complement(3145..4503)
                     /locus_tag="PKJ61_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOC13567.1"
                     /translation="MSELRDALEAAHRIALDWLNSLPERRVPPSAGVDQVAAALGDLP
                     DGPQPSPAVVEQLAHAVGPGLTAMPSPRWFGMVIGGTHPAGLAADWLTSAWDQNSALR
                     AVTPGTIAVEEVVDRWLLDLLGLPEDAGVGLVTGATMSNWTCLVTARDTVLRRAGWDV
                     TEHGLAGSPGVRVLVGAERHDSIDSALRYAGMGRPEPVGVDAQGRIDTAALVAALKQR
                     PGRPTVVALQAGNLHSGAFDDFPAAIEAAHAAGAWVHIDGAFGLFAAASARYRHLVAG
                     FAEADSWTTDAHKTLNVPYDCGIAIVRDDAAMRAAMGMHGSYLIQDAAGDPFDRVPEM
                     SRRARATPLWAVLAALGRSGVAELVERYCRHAQRFAEGIAAIPGAEVLNDVVFTQVCA
                     SFGSDEHTNEVVRRMLADGTAWTSGSKWHDRAVLRVAVCNWSTTDVDVDATLAALHQA
                     AT"
     gene            complement(4601..6079)
                     /locus_tag="PKJ61_06795"
     CDS             complement(4601..6079)
                     /locus_tag="PKJ61_06795"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020392155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOC13568.1"
                     /translation="MPLDFARLGVPTNLVTVLAQRDITIPTPIQAATLPDSLAGRDVL
                     GRGRTGSGKTYAFLLPLVARLTAKPKRAAARRPRALVLAPTRELATQIAESLEPLASA
                     TGLTTRTVFGGVGQNPQVRALERGVDVLIACPGRLLDLMDQGHVILDAIEITVIDEAD
                     HMADMGFLPMVRRVLDKTPGQGQRMLFSATLDAGVDVLVKRFLHSPVVHEADSSAPVG
                     EMEHHLLGVSAAERGDVLAELAAAPGRTILFTRTKHGAKKLTKQLIGRGIPAVELHGN
                     LNQNARTRNLDAFHHGLVETLVATDIAARGIHVDDVALVVHADPPAEHKAYLHRSGRT
                     ARAGQSGTVITLVTPDQRHEVAALMRAAKIKPTTTAVNPGDATLASLAPGERTYLPAA
                     EAMALANPNAQPADQRQQPAERRGSAGGRSSGRRGDSGGRSTARQGDSGRNRSERTRP
                     EAKQGAGSGQGTARRNGSRPASSGANGGAAAFSASSGRGRRR"
     gene            6440..7984
                     /locus_tag="PKJ61_06800"
     CDS             6440..7984
                     /locus_tag="PKJ61_06800"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016669930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HOC13569.1"
                     /translation="MGDQLEQLRRKRQSALAGGGQERVEAQHARGKLTARERLAILLD
                     EGSFQELGALATHQSSEFGMDKLRFPGDGVITGFGKVNGRRVAVYAQDFTVLGGSFSE
                     VQSQKICRMMDLAIDAGIPLIGLNDSGGARVQEGVKSLAAYGEVFYRNVAASGVVPQL
                     SLVMGPCAGGAVYSPALTDFTIMVEHTSNMFLTGPGIIKAVTGEEVTAEDLGGAWVHN
                     ARSGVSQFSAETDQAAIGLAKLLLGYLPQNNTEDPPALTPYDDPARMDESLNTIMPTD
                     DTLPYDMRDILELVFDAGSILENHEQFAPNAITAFARLDGNSVGVVANQPAVLSGSLD
                     LDSSDKIARFVRICDMFNIPLVTFVDCPGYLPGVDQEHNGVIRHGAKIIYAFAQATVP
                     KLSVITRKAIGGSYVAMSSKQMGNDIAFAWPSAQIAVMGAEGAARLLHSKAIAAAEDP
                     AKAEDEFIREYKERLFNPYHAADVGQIDEVIEPRETRLRLIRALEVLRTKVHTNVPKK
                     HGLFPV"
     gene            7994..8353
                     /locus_tag="PKJ61_06805"
     CDS             7994..8353
                     /locus_tag="PKJ61_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016669931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family protein"
                     /protein_id="HOC13570.1"
                     /translation="MGEIGFGLYLAAVGMGTVFGVLLVLMLVLKLIGRLDRSTSVRTR
                     IAPEPDVGTEPTADQTAADGLTPELVAAITVAVVTHARVRRSQAAPAMRRVAPGSQLF
                     ASRWVAVGRGYQNQPWK"
     gene            8360..8842
                     /locus_tag="PKJ61_06810"
     CDS             8360..8842
                     /locus_tag="PKJ61_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016669932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HOC13571.1"
                     /translation="MRRYTVRINDTEHVLDVEELAADTFTVHLEDGRLVDVILTDHQD
                     LAQAVIAPQLEIGASRVAVPAHHSQVRPVESAAPSRPLPARSAVAGNTVAAPMPGVIL
                     SVEVTPGAAVRRGQTVLVLEAMKMKNELKAQRDGTVARVAVAAGDQVKHGDVLVEFEA
                     "
     gene            8847..9968
                     /locus_tag="PKJ61_06815"
     CDS             8847..9968
                     /locus_tag="PKJ61_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015068986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HOC13572.1"
                     /translation="MSQDTIDQLLAGITSVTWQSLVMMAIGGLLIFLAISREYEPLLL
                     LPIGAGAILANLPLSPLVADGGLLTILYDIGVGNELFPLFIFIGIGALTDFGPLLENP
                     KMVLLGAAGQFGIFLTVILALLLGFDRNQAASIGIIGAIDGPTSIYVSGRLAPELLGP
                     ITVAAYSYMALVPLIMPPVMRLLTTKAERSIRMPYTQREISQRTRVLFPIVVTVAVGI
                     VVPFATPLIGCLMLGNLMRESKVVERLTHASANELANVVTLFLGLAIGATMQGEAFLQ
                     VGTLAILGLGLIAFFLDTATGVLFGKLMNVFSGGKFNPLIGAAGISAFPMAARVVQKE
                     ASREDFSNFVLMHAMGANAAGQIASVVAGGVLLALMGAG"
     gene            complement(10053..11165)
                     /gene="ald"
                     /locus_tag="PKJ61_06820"
     CDS             complement(10053..11165)
                     /gene="ald"
                     /locus_tag="PKJ61_06820"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012922689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HOC13573.1"
                     /translation="MRIAVPSEVKNHEYRVAITPVGVAELTAHGHEVFVQQGAGLGSS
                     ITDAEYVAQGAKILPDAATTWAAGEMVLKVKEPIESEYYFLRDDLLLFTYLHLAADRP
                     GTDALLDAGTTAIAYETVQLPSGLLPLLYPMSEVAGCLAPQVGAHAMMKAQGGRGVLM
                     GCVGGVANAKVVVLGGGTAGQNAANVALGMGADVTILDTDLDKLRTTFWRYDNRVHQI
                     VSSRLSIREQVLAADMVIGTVLIPGAKAPKLVTNELVAQMKPGSVLVDVAIDQGGCFE
                     DSRPTTHADPTFMVHDSVFYCVANMPGAVPNTSTWALTNATLAYALQLADKGWQGACR
                     ENRSLALGLNTHAGQIVYPAVAEACQLPFTPLDDLL"
     regulatory      11286..11418
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            11512..12261
                     /locus_tag="PKJ61_06825"
     CDS             11512..12261
                     /locus_tag="PKJ61_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOC13574.1"
                     /translation="MKREVLAPIGLGALLLIGWKLTAGGGSVLLPTPGAVAARLVTDL
                     TDPRYWGYLLVTLAEAFGGALLGAAVALPTAIGIHRSRWAAAAVHPFLGATQAIPAIA
                     LAPLLVLWLDYGLFSIVVLCALIVFFPILIAAVIGLKHVDADVVDAGRIDGASSLALL
                     YFVELPMALPSILGGLRNGVTLAVTGAIVGEMVMGGSGLGQVLTIQRGSVDTAGMFAT
                     IIVLCGFASSAYALILLWERRSRIVNSLQRA"
     gene            12267..13277
                     /locus_tag="PKJ61_06830"
     CDS             12267..13277
                     /locus_tag="PKJ61_06830"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOC13575.1"
                     /translation="MPRRILAGLVALLALSACAPATQQSSVSATAVATATPTIGLTYI
                     PNVQFSPFYLADADGDFARAGLAPTLRHHGASEGLFTAIAAGQEQFVIAGGDEMLQAR
                     AQGLDLIAVGAYFHEYPAVVIVPEASAIGSLADLKGHSIGIPGKYGENWFALLVALRT
                     AGLTEADVEIKEIGYTQQAALSQGKVDAIVGFSNNEAVQFALGGLATRSLAIADGQVP
                     LVSICLITTSEYAKANPDVVRRVATAMLGGVKTAVTDPDHALAASAKFVPGLSASAAQ
                     AAAKATLVATAKLWVDASGEVSGKLDPDQWTAMAAFMTDHGLLTGDADAAAAMSNEYL
                     PK"
     gene            13344..13787
                     /locus_tag="PKJ61_06835"
     CDS             13344..13787
                     /locus_tag="PKJ61_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015071327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HOC13576.1"
                     /translation="MNEHYVPGWDEYFLGIASAVAARAKCTRRRVGAVLVHDRRIIAT
                     GYNGAAPGRPDCLEGACPRGQLDYDQVPGLGDYDRPGTPGFCIAIHAEVNALLFATRD
                     TKGATAYITDPPCPGCRKALAAAGIVRAVWPDGEHDTDGLVNWDA"
     gene            complement(13820..14407)
                     /locus_tag="PKJ61_06840"
     CDS             complement(13820..14407)
                     /locus_tag="PKJ61_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020385546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HOC13577.1"
                     /translation="MNIRRLRNGVAALSAVIGLLPAFTASQALAVPSADSAGSANPPA
                     ATRTTTLKPAELAIYREALLNKQSTAIAVQNATIEAIHAQTVAELGYEPATTDPREIA
                     RQIMLNKYEWGEDQFACYDSIIMRESRWIVTADNPYSSAYGIPQALPGKRMAEFGDDW
                     RTNPATQIRWGLNYVDERYGTPCQAWSFKRGHGWY"
     gene            14502..15596
                     /locus_tag="PKJ61_06845"
     CDS             14502..15596
                     /locus_tag="PKJ61_06845"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOC13578.1"
                     /translation="MQLRVVEGADVVGALADALAGGPPLAPLPEARQLPALQIDEPVT
                     EPDAGAVVTTSGATGAPKAVVLSRSALRFSADATHARLGGAGDWVCALPTRHVAGLMT
                     VVRAVRAGTAVHFARPDLADLETPRTRTYISLVAAQLDRALHEAETVAKLGAFAAVLL
                     GGSALPSGLRERANDAGVKLVATYGMSETCGGCVYDGVPLDGVRVELEGERISLGGPM
                     AFLGYRGRPELTRTVLSGDLVRTSDRGIWADGRLRVLGRADDVVISGGENVDLAAVQH
                     ACDAEFGTGTLAVLAIPDARWGAKVVALSRADLDLGRVQAILEPRLGKAAAPKELRRV
                     EHLAYTSIGKIDRTALQLSWRELGEDGDVG"
     gene            15580..16458
                     /locus_tag="PKJ61_06850"
     CDS             15580..16458
                     /locus_tag="PKJ61_06850"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015582117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HOC13579.1"
                     /translation="MATLAEWVEGARPRTLPAALAPVLAGSAVAWFEGGFDPLLATLA
                     LVVALALQVGVNYANDYSDGIRGTDAVRVGPMRLVGSGAAKPAHVKLAAFACFGVAAV
                     AGLTVVVITGHWWLLGLGAAAILAAWFYTGGRKPYGYLGLGELFVFVFFGLVAVSGTV
                     YIQVGTVSASAWWTAVAIGALACAILVANNLRDLDGDRAVGKRTLATRLGDSGTRWFF
                     VGLVALAAVGVFGVAATSSWWALLGWLMLLLLAGPMLEVVRGVSGYGLIHVLKRTGLA
                     ELACAAGLFVGLLIAR"
     gene            16462..17568
                     /locus_tag="PKJ61_06855"
     CDS             16462..17568
                     /locus_tag="PKJ61_06855"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020391911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HOC13580.1"
                     /translation="MGRNSAVAFALPMRNRFRGLTVREGLLVHGPAGWGEWSPFAEYE
                     RPEIDNWWRATLEAANLGFPEPVRDRVPVNATVPAVDPETAYAIVAGSGCGTAKVKVA
                     EPGQSGTEDASRLEAVRDALGPSGRLRIDANGAWDVDTALSRLAELAQFGLEYAEQPC
                     ATVDELAELRRLLARRGFQVPIAADESIRRSGDPERVVALEAADIAVLKVQPLGGVRA
                     CLELAERLGLPVVVSSALESSVGLAAGVALAAALPELPYACGLNTATMFTMDVTDRPL
                     VAVDGAIPVRTVSPDRHPCAAPTTADRGPGAALSATADRHLGVGRDLGSQPVVGGAQD
                     VRADDWRAGPEATARWLARLGPMAEELIEPGEGR"
     gene            17565..>18647
                     /gene="menD"
                     /locus_tag="PKJ61_06860"
     CDS             17565..>18647
                     /gene="menD"
                     /locus_tag="PKJ61_06860"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HOC13581.1"
                     /translation="MTDSVACASAVLGQLLAQGVTDLVLAPGSRSAPLALVGDAAARA
                     GRLRLHVRVDERSAGFLALGLAKASGRCVPIVTTSGTAVGNLLPAVMEAHHSGVPLLV
                     VSADRPAALVGFGANQTTEQSQLFDGFLRYRARVSSAASAVSWPAQAARAVLAATGAL
                     SRFPGPAQLNVELGQPLVSDAVEAPAEFRPVLTPDQPDAVVHPLPGAPRTLVVCGDAP
                     VSIGRVAVELAEAAGLPLLAEPSSNARTGANALGHGRILLAGPLAEQIERVIVFGRPN
                     LSRPVTALLSRADIEVIVLSASPDFVDPGWRASVVAGAVSVAPGPPDWLAAWQDADSR
                     LRPVALGDPGLRRDDGGLGLRRDDGGL"
     assembly_gap    18648..18747
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <18748..19301
                     /locus_tag="PKJ61_06865"
     CDS             <18748..19301
                     /locus_tag="PKJ61_06865"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HOC13582.1"
                     /translation="GPQVAALVVASVPADQVLCLGNSNPIRDADLAPVRPDSPPVFAN
                     RGLSGIDGTLATAIGVALGSGRPTTLLCGDLSFVHDVGALAIGPGEPRPELRIVVADD
                     AGGSIFATLEYGGEQFADSFERVFATPTGLDLVALTRGFGIPCVRISTADELASALDE
                     PVRGLEVLVVDVDRSGRREPAPA"
     gene            19546..>20240
                     /locus_tag="PKJ61_06870"
     CDS             19546..>20240
                     /locus_tag="PKJ61_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HOC13583.1"
                     /translation="MTDTNTPLGLMTQRGPTVLWNDSATYTELSTAISWGAVGATCNP
                     VIALAAIRADLPRWTARIKELAAEMPDATESQIGWKVVEEVSLDAAKLLEPAFEKYQG
                     YNGRLSMQTDPRLARSAKALADQAEYFSNLAPNIIVKIPATKTGVEAIEDATRRGVNI
                     NVTVSFTVAQAVAAGEAIERGLKAREAAGEDVSKMGPVVTIMVGRLDDWLKQVYAKDG
                     LVFDPGYLEWAGVA"
     assembly_gap    20241..20340
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            20509..22407
                     /gene="iolD"
                     /locus_tag="PKJ61_06875"
     CDS             20509..22407
                     /gene="iolD"
                     /locus_tag="PKJ61_06875"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002513196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016823 - hydrolase activity, acting on
                     acid carbon-carbon bonds, in ketonic substances [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HOC13584.1"
                     /translation="MTQTVRLTVAQAVVKFLANQYTERDGEEQKLFAGCFGIFGHGNV
                     AGVGQALLQAELQQLDGNPDAPDLPYVLVRNEQGAVHAAAAYAKTKNRLQTWAVTSSI
                     GPGATNMVTGAALATTNRLPVLLLPSDIFATRYPDPVLQQLEDPRTLDISVNDAFKPV
                     SRYWDRINRPEQLIGSLLAAMRVLTDPAETGAATLCLPQDVQAEAFDFPVELFGKRVW
                     HVARPVPEPAALARAVAAIKAAKRPLVVAGGGVIYSGGSDALRAFADATGIPVSDTQA
                     GKGAINFDHRMAVGGVGSTGGSAANTLAREADLVIGIGTRYSDFTTASHTIFADPNVK
                     FVNVNVLAFDAAKHAATMVVADAKSALEALTAELSGWSTDAAYQARAEQLDAEWQAVV
                     DECYHRDNQPLPAQTEVFGALNELMGEKDVVINAAGSMPGDLQALWRARTPEQYHLEY
                     AYSCMGYEIPAALGVKLAVGDEHEVVAIVGDGTYQMLPMEIATMAQEGIKVILVLLQN
                     HGFASIGALSESRGSQRFGTKYKTRSTSGRLDGADVPFDLAANAASWGADVIRCNGIA
                     EFRENYPKAVANDRTTVLYIETNLYGPNPPGTAWWDVPVSQVSELASTQQAYGEYIAE
                     KSAQRHYL"
     gene            22408..>22997
                     /locus_tag="PKJ61_06880"
     CDS             22408..>22997
                     /locus_tag="PKJ61_06880"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOC13585.1"
                     /translation="MNALSNLWASLPLWGWLALVLCALLIGFAKTAIGGVAMLAVVLA
                     AIVLPAKDSTGVVLVMLLVGDLVAVWAYRHNVDWKLIGRLVLPVLVGIALGAWFLNWV
                     DGPTLKRTIGVILLLLLVLGLFRNRLAVHHPAVGAAYGGLAGFTTMVANAGGPPMNLY
                     LLAAKYDKWRFLGTTAWFFFAVNLTKLPVSISQSIVR"
BASE COUNT         3382 a         7684 c         7946 g         3785 t
ORIGIN      
        1 ttgggcatgg gcttcgacaa gcccgatctg ggcttcgtga ttcacctggg cgcgcccagc
       61 tcgccggtcg cctactacca gcaggtgggg cgcgccggtc gcgcggtggc cagcgccgac
      121 gtcctgctgc tgccgacacc ggcggatcgg gagatctggc actacttcgc gacggcttcg
      181 atgccggacg aggcaaaggc gtcggcggtg atcgaagccc tggccagcgc cggctcgccg
      241 atgtcgactg ccgcgctgga ggcggtggtc gacatccggc gtgcccgact cgaactgctg
      301 ctgaaggtgc tggacgtgga cggcgcggtg acccgggtcc gcggcggctg ggctgccact
      361 ggggcgccgt gggtgtacga ccgggagcgt tacgcgcggg tggcggcggc acgcgagcgg
      421 gaacagcagc tgatgctcga ctacgtggac accagcggct gccgcatggc attcctgcag
      481 cagacactgg acgatcccga accgacccgg tgcggacgct gcgatcggtg cgccgagacc
      541 tggtacgccg actcggtgtc gagtgctgcg gcggagtcgg ctcgcgccga gctgggtcgc
      601 gcgggggtgg aactggtgcc gcgaacgctc tggccgtccg gcatggcgaa gcttggggtg
      661 ccggtctcgg gaaggatcgg cgcgagcgac gctgccgagc ctggccgcgt gctcgcccgg
      721 ttcaccgacc tcgggctcgg gcagcggctg cgtaacctgc tcgccgaacc ggacgcgccg
      781 gtacccgact gggtgctgcc gtactgcttc gaagtgctgc gcgagtggga ctgggctgaa
      841 cggccgaccg cggtgcttgc ggtgccgtcg ctgacccacc cgcagctggt ggcaacgctc
      901 gctgccgagc tggctcgcag tggccggctg accgatctcg gttcgctggc actgcgctcg
      961 accgaaatgg aggagcgggc gggcaacagc gccttccggc tgaaacagat cttcagtcgg
     1021 ttcacggtca cgcccgacca ggccgacgcg ctggttgaag ccggcccggt gctgctggtg
     1081 gacgacctgg tcgactcccg ctggacgatc acagtggccg ccagcctgct gcgccaagcc
     1141 ggagccactt cagtcctgcc cttcgcgcta gccgccgtcg cctgagcatc cgccaagtac
     1201 tcggtccctg aggagcgttc gtgaggaacg aacgagcgcg tcacgaaggg gcgctgtcac
     1261 cgttttgccg cttgtgttgt tcgagcttgt cgcgttcggg tggtcaagtt gcgctcgggg
     1321 tgttgggtgc tctccctgga atcggtccct gaggagcgtt cgcgaggcac gagcgagcgc
     1381 gtcacgaagg gccgccgcca tctcgtaatc ggtaagtagc aaatcacaaa aaagcttgtg
     1441 aaaatactac agtgagcata gaatagaact tatggcgagc actgtagacg aagatctcag
     1501 cgcactgctc gacgcctaca tagcaaccgc tctggtagca gatccgcccc cggagctgcc
     1561 gttgtggcag tacaccgagc gtgagttgct ggccgagctg aagcgggtcc agcagcgctc
     1621 tgatgcggtc gccgcgcgcc agcttgcgct gctggctgag gcggagcgcc gtgaatctac
     1681 ggtccatcag gcttcgctgc ctaccgccgg ctggctggtc gaccacaaca cgcactcggc
     1741 ccggtccgca cgtgaagaag tgcagttcgc ggtcgcgctg gcccaatcgc caagcgtggg
     1801 ccgagctctc gaacgtggcc aggtatcagt tgagcaggcc aaggcgatcg tcaacggttt
     1861 gcgccggttg cccgaagatc tggacgattc ccggcgccag ctggtcgcgg gccaattggt
     1921 caggttcgct gccgacttcg gtccggccgg cttggcccgg ctggtcaatc gggcgatcga
     1981 agtcgtggcg cctgaagtgg ccgaagatgc ggatcgtcgc tcggtggagc ggctggacgc
     2041 ggagcagcgt agggatcggt acttcacttg gcggcaaaac ccggacggtt cgtggacctt
     2101 ccacggaaag ctcccgtccg tccctggcca gcagttgatc aacgtggtca gggctgtcgc
     2161 caagccgcac cggtccagcg tgctgcttgc cggtggccag atcaccggct cccaagctaa
     2221 cgcggacgcg ttgtgcgggc tagtcgagca ctacgcctcc tgcggcatcg cccctgacca
     2281 tggggcggat cggccgcgcg tcatcgtgac catcgacttc gacacccttc ggggcaagct
     2341 gggcaccgcc acgctgctgg acgctgattg caggatcact gctcaagagg ctcggcggat
     2401 cgcctgtgac gcgcacatcc tgcccgccgt cctcggctcg aagagcaagc cactggatgt
     2461 gggccgctcg aagcggttgt tcaccaagga actgcgccac ctgctgatcc tgcgtgatcg
     2521 gggctgcacg ttccccggtt gcgaccggcc gccgtccgag tgcgaagccc accaccgcaa
     2581 gccctggtgg gctgggggcg agaccagcct ggacagcggg gtgctgttgt gcaccttcca
     2641 tcaccacaca gtggaaccca accccaacct gccgattgaa tcccagtggc tcatccggct
     2701 ggacgaccgc ggcctgtccg agttcgccgc accattgcgg cccaatcagg ccgagcgggt
     2761 ttggaagcaa caccaccggt tccgcagctg aacgtggcct gtcctagacc gggaagcact
     2821 gatcaacgat ccctgagccg ggcgttgggt cgtccattcg ctcccggcta cgtgcttcgt
     2881 caagctcagc cagcgttggt cggcatcgat cagcggttcc ctgttgggct cagcacttcg
     2941 ggtggtcgag cctgacctgg ctgggtttct agaaggttat agatcacagg cggcggccct
     3001 tcgtgacgcc atgctcgttc ctcgcatggc tcctcaggga ccgttgctgg ggccggcccg
     3061 ttccttgtat ggcttctcag gtgccatttg ttgggtccgg ctcgttcctc gcatggctcc
     3121 tcagggactg ttggtgtccg cgacctaggt ggctgcttgg tgcagcgcgg ccagggtcgc
     3181 gtcgacgtcc acgtccgtgg tcgaccagtt gcacacggcc acgcgtagga cagcgcggtc
     3241 atgccacttc gaacctgagg tccaggcggt cccgtcggcg agcatgcgac gcacgacctc
     3301 gttggtgtgc tcgtcgctgc cgaagctggc gcagacctgg gtgaacacca cgtcgttcag
     3361 cacctccgcg cccggaatcg ccgcgatgcc ctcggcgaag cgctgggcat gtcggcagta
     3421 gcgctcgacc agctccgcga caccgcttcg gccgagcgcc gccagcaccg cccagagcgg
     3481 ggtcgcccgc gctcgcctcg acatctccgg tacgcggtcg aacgggtcgc cggcagcgtc
     3541 ctggatcagg tacgagccgt gcatgcccat cgctgcgcgc atggctgcgt cgtcgcgcac
     3601 gatcgcgatt ccgcagtcgt agggcacgtt cagtgtcttg tgcgcgtcgg tggtccagga
     3661 gtccgcctcc gcgaatccgg ccacgaggtg ccgataccgt gcggacgcgg ccgcgaacag
     3721 cccgaaggcc ccatcgatgt gtacccacgc cccggcggcg tgcgccgctt cgattgctgc
     3781 cgggaagtcg tcgaaagctc ccgaatgcag gttgccggcc tgcagcgcca cgacggtcgg
     3841 acggcccggt cgctgcttga gggcggccac tagcgcggcc gtgtcgatcc gcccctgggc
     3901 gtccactccg accggctccg gccggcccat gccggcgtag cggagtgcgg agtcgatgga
     3961 gtcgtgacgt tcggctccga ccagcacccg gacgccgggc gatcccgcca agccgtgttc
     4021 ggtgacgtcc catcccgctc gccggagcac ggtgtcgcgg gcggtgacca ggcaggtcca
     4081 gttcgacatg gtcgctccgg tgaccaggcc gacacccgca tcctcgggca ggccgagcag
     4141 gtccagcagc caacggtcga ccacctcctc gaccgcgatc gtcccgggag tgaccgcgcg
     4201 cagcgcgctg ttctgatccc acgccgaagt cagccagtcc gcggcgagcc cagctggatg
     4261 ggtgccgccg atcaccattc cgaaccagcg cggggacggc atcgcggtca gccctggccc
     4321 gacggcgtgc gccagttgct cgaccacagc gggcgatggt tgggggccat ccggtaaatc
     4381 gcccagggca gccgccacct gatccacccc cgcgcttggc ggcactcgtc gctcaggcag
     4441 ggagtttagc cagtccagcg cgatgcgatg ggcggcttcc agtgcatccc ggagttcaga
     4501 catggccacc tcctgcggcc atccttcacc cagcgcggac gttgatcgcc gcgaagttgt
     4561 gcttatcccg tgccagcgtc ctcggtgatc cggccttggc tcagcggcgt cgcccgcgac
     4621 ccgaggacgc agagaaggca gccgcgccgc cgtttgcacc gctgctcgcc ggccgagagc
     4681 cgttccgcct cgcggtgccc tggccggacc cagcaccctg cttggcctcc ggacgcgtcc
     4741 gctcggagcg gttgcgtccg gaatcaccct gacgagcagt cgaacgacca ccggagtcgc
     4801 cacgtcgacc cgaggatcgg ccgccggccg agccgcggcg ttcagccggc tgctgccgtt
     4861 ggtcggccgg ttgggcgttc gggttggcca acgccatcgc ctcagccgcc ggcaggtacg
     4921 tccgctcgcc cggggcgagg ctggccaagg tggcgtcgcc cgggttcacg gccgtcgtcg
     4981 tcggcttgat cttggccgcg cgcatcagtg ccgccacttc gtggcgctgg tcgggcgtga
     5041 ccagggtgat cacggtgccc gactggccgg cccgcgcggt gcgtccggag cggtgcaggt
     5101 acgccttgtg ctcggccggc ggatcggcat gtacgaccag tgcgacatca tccacatgga
     5161 tgccgcgcgc ggcgatgtcg gtcgccacca gcgtctccac caagccatgg tggaacgcgt
     5221 ccaggttgcg ggtgcgggcg ttctggttga ggttgccgtg cagttccacc gccggaatgc
     5281 cgcgtccgat cagctgcttg gtgagcttct tcgcgccgtg cttggtgcgg gtgaacagga
     5341 tggtccgccc gggggccgca gcgagttcgg ccagcacgtc gccgcgctcg gccgcgctca
     5401 ccccgagcag gtgatgctcc atctcgccga ccggggcgga cgagtccgcc tcgtgcacga
     5461 cgggggagtg caggaagcgc ttcacgagca catcgacacc cgcgtcgagc gtggccgaga
     5521 acagcatccg ctgtccttgc cccggcgtct tgtcgaggac tcggcgcacc atcggcagga
     5581 agcccatgtc ggccatgtgg tcggcttcgt cgatcacggt gatctcgatg gcatcgagga
     5641 tcacgtgacc ctgatccatc aggtcgagca ggcgtccggg gcaggcgatc agtacgtcca
     5701 cgccacgttc gagcgcgcgc acctgcgggt tctggccgac tccgccgaac accgtccgag
     5761 tggtcaggcc ggtggcggac gcgagcggtt cgagcgactc ggcgatctgg gtggccagct
     5821 cacgggtggg cgccagcacg agggcgcggg gacggcgagc ggccgcccgc ttgggcttgg
     5881 ccgtcagtcg ggcgaccagc ggcagcagga acgcgtaggt cttgcctgag ccggtgcggc
     5941 cacggccgag cacgtcccgg ccggccagcg aatcgggcag cgtggcggcc tgaatcgggg
     6001 tagggatggt gatgtcccgc tgtgcgagca cggtcaccag gttggtgggc acgccaaggc
     6061 gtgcgaaatc aaggggcacg aaaaatccgt ccgaatcggt gtcttgcctg gcgcgctcgg
     6121 ctgggtccgt cccgtggacg gtgcggcgcg gccagcggac tcagtaatcc gcatcaccgg
     6181 gcgactcgag gcaagactgg tagccgagtc gctggtagcg atcttacgcc attgaaacgt
     6241 caactgtcgc atcgagggtc gtcagggtca tgtcgcgaac tcgaagccga agccgcttcc
     6301 cgcatcgatc cgagtgggga atcctcacac agcagcgaga ttgcgacacc gccggttttc
     6361 ccgtcctgac cccgctgcga ggtacctggc gtagggtcgc agacaagcgg atccggatgc
     6421 caggacgggt gagcaggcga tgggcgacca actcgagcaa cttcgacgca agcggcaatc
     6481 ggcactggcc ggtggcggtc aggaacgggt cgaggcccag cacgcccgcg ggaagctgac
     6541 cgctcgtgag cggctggcca ttctgttgga cgagggctcc ttccaggagc tgggagcgct
     6601 ggccacccac cagtcgtccg agttcggcat ggacaagctg cgtttccccg gcgacggcgt
     6661 gattaccggc ttcggcaagg tgaacggacg ccgggtggcg gtctacgccc aggacttcac
     6721 cgtgctgggc ggttcgttct ctgaggtcca gtcgcagaag atctgccgga tgatggacct
     6781 cgccatcgat gccggcattc cgctgatcgg tctgaacgac tccggtggcg cccgcgtcca
     6841 ggagggtgtg aagagccttg ccgcctacgg cgaggtgttc taccggaacg tggcggcgtc
     6901 cggggtggtg ccccagctct cgctggtcat ggggccgtgc gccgggggcg cggtctattc
     6961 acctgcgctc accgacttca cgatcatggt cgagcacacc tcgaacatgt tcctcaccgg
     7021 gccgggcatc atcaaggccg tcaccggtga ggaggtcacc gccgaggacc tgggcggtgc
     7081 ctgggtgcac aacgcccgca gcggggtctc ccagttctcc gcagagaccg accaggccgc
     7141 gatcggactg gccaagctgt tgctcggcta cctgccgcag aacaacaccg aggacccgcc
     7201 cgcgctcacc ccgtacgacg atccggcacg catggacgag tcgctgaaca cgatcatgcc
     7261 caccgacgac accctgccct acgacatgcg cgacatcctg gagctcgttt tcgatgccgg
     7321 cagcatcctg gagaaccacg agcagttcgc gcccaacgcg atcaccgcat tcgcccgttt
     7381 ggacgggaac tccgtcggtg tggtggccaa ccagccggcg gtgctgtcgg gctcgctcga
     7441 cctggattcc tcggacaaga tcgcgcgctt cgtccgcatc tgcgacatgt tcaacatccc
     7501 gctggtcacc ttcgtcgact gccctggcta ccttcccggc gtcgaccagg agcacaacgg
     7561 ggtgatccgg cacggggcca agatcatcta cgcgttcgcc caggccaccg tcccgaagct
     7621 gtcggtgatc acccggaagg cgatcggcgg ctcctacgtg gcgatgagct ccaagcagat
     7681 gggcaacgac atcgccttcg cgtggccgag tgcgcagatc gcggtgatgg gtgccgaagg
     7741 ggccgcccga ctgctgcaca gcaaggcgat cgcagcggca gaagacccgg cgaaggccga
     7801 ggatgagttc atccgcgagt acaaggagcg ccttttcaac ccctaccacg ccgccgacgt
     7861 cggccagata gatgaggtga tcgagccccg ggagacccgc ctgcggctga tccgggcact
     7921 ggaggtgctg cgcaccaagg tgcacacgaa cgtgcccaag aagcacgggc tcttcccggt
     7981 ctaggtggac gacatgggcg agatcgggtt cggcctctac ctggccgcag tcggcatggg
     8041 gacggtcttc ggcgtcctgc tggtgctgat gctggtgctc aagctgatcg gccggctgga
     8101 tcggtcgacc tccgtgcgga cgcggatagc gcccgagccg gacgtgggaa ccgaacccac
     8161 cgccgatcag acggcggccg acggcctcac cccggagttg gtcgccgcga tcacggtagc
     8221 tgtggtcacc cacgcccggg tgcggcgcag ccaggccgcc ccggccatgc gccgggtggc
     8281 tcccggcagc cagttgttcg ccagccgctg ggttgcggtc ggccgcggct accagaacca
     8341 accctggaag tgaggacgca tgcgtcgcta caccgtacgg atcaacgaca ccgagcacgt
     8401 gctcgatgtc gaggaactgg ctgccgacac cttcaccgtg cacctggagg acggtcggct
     8461 ggtggacgtg atcctcaccg accaccagga cctcgcccag gccgtgatcg ccccgcaact
     8521 ggagatcggc gcgtccaggg tggccgtccc agcgcaccat tcgcaggtgc ggccggtcga
     8581 gtcggccgcg ccatcgcgtc cgcttccggc ccggtctgcg gtggccggca acaccgtcgc
     8641 cgcaccgatg cccggggtca tcctgagcgt cgaagtcacg cccggggcgg ccgtgcggcg
     8701 cgggcagacc gtgctcgtgc tggaggcgat gaagatgaag aacgagctga aggcgcagcg
     8761 ggacggcacg gtggccaggg tcgcggtggc cgcgggtgac caggtgaaac acggcgacgt
     8821 gctggtcgag ttcgaggcct gagccgatga gccaggacac gatcgaccag ttgctggccg
     8881 gcatcaccag cgtcacctgg cagtcgctgg tgatgatggc gatcggcggg ctgctgatct
     8941 tcctggccat ctcgagggag tacgagccgc tgctgttgct gccgatcggc gcgggcgcga
     9001 tcctcgccaa tctacccctg tcaccgctgg tcgcggacgg cggcctgctc acgatcctct
     9061 acgacatcgg ggtgggcaac gagctgttcc cgctgttcat cttcatcggc atcggggcgc
     9121 tcaccgactt cgggccgctg ctggagaacc ccaagatggt gctgttgggg gcggccgggc
     9181 agttcggcat cttcctgacc gtgatcctcg ccctgctgct gggcttcgac cgcaaccagg
     9241 ccgcctccat cggcatcatc ggggcgatcg acggtcccac gtccatctac gtcagcggac
     9301 gccttgcacc ggagctgctc ggcccgatca cggtggccgc ctatagctac atggcgctgg
     9361 tgccgctgat catgccgccg gtgatgcggc tactcacgac gaaggctgag cggtcgatcc
     9421 gcatgccgta cacccagcgc gagatcagcc agcggacgcg ggtgctcttc ccgatcgtgg
     9481 tgaccgtggc ggtggggatc gtggtgccgt tcgcgacccc gctgatcggc tgcctgatgc
     9541 tgggcaacct gatgcgggag tcgaaggtgg tggagcggct cacccatgcg tccgcgaacg
     9601 aactggccaa cgtggtgacg ctgttcctcg gcctggcgat cggggccacg atgcagggcg
     9661 aggctttcct gcaggtcggg acgctggcga tcctcgggtt ggggctgatc gccttcttcc
     9721 tggacaccgc taccggagtg ctgttcggca agctgatgaa cgtgttctcc ggcggcaagt
     9781 tcaacccgct gatcggggcg gcgggtatca gcgccttccc gatggctgcc cgggtagtgc
     9841 agaaggaggc cagtcgcgag gacttctcca acttcgtgct catgcacgcc atgggcgcca
     9901 acgctgccgg ccagatcgcc agcgtggtcg ccggtggtgt gctgctggca ctgatgggcg
     9961 ccggctgacg ccgccatcga aatgcgggtt tcaagcgcct tctggcgcgc catcgcccgc
    10021 atttcgatgc ccgagcggtt tccggcaggc tcctagagca ggtcgtcgag cggcgtgaac
    10081 ggcagctggc atgcctcggc caccgccggg tagacgatct gaccggcgtg ggtgttcagc
    10141 ccgagcgcca gcgaacggtt ctcgcggcag gcgccctgcc accccttgtc agcgagctgc
    10201 agcgcatagg cgagggtggc gttggtgaga gcccaggtgg aggtgttcgg caccgcgccg
    10261 ggcatgttcg ccacgcagta gaacaccgag tcgtgcacca tgaacgtcgg gtcggcgtgg
    10321 gtggtcggac gggaatcctc gaagcagccg ccctggtcga tggccacgtc caccagcacc
    10381 gagccgggct tcatctgtgc gacgagttcg ttggtgacca gtttcggcgc ctttgcgccc
    10441 gggatcagca ctgtgccgat caccatgtcg gcggccagca cttgttcgcg gatgctgagc
    10501 cgggacgaca caatctggtg cacccggttg tcgtagcgcc agaaggtggt gcgcagcttg
    10561 tccagatcgg tatcgaggat ggtcacgtcc gcgcccatcc cgagtgcaac attggcagcg
    10621 ttctggccgg ccgtgccacc cccgagcacc accaccttgg cgttggccac gccgccgacg
    10681 cagcccatca gcaccccgcg tccgccctgg gccttcatca tggcgtgcgc gccgacctgg
    10741 ggcgcgaggc agccggccac ctccgacatc gggtagagca gcggcaacag cccgctcggc
    10801 aactgcaccg tctcgtaggc gatcgcggtg gtgccggcgt caagcagcgc gtccgtgccc
    10861 ggacggtcgg cagccaggtg caggtaggtg aacagcagca ggtcgtcacg caggaagtag
    10921 tactcgctct cgatcggctc cttgaccttc agtaccatct cgccggcggc ccaggtagtg
    10981 gctgcatccg gcaggatctt cgcgccctgg gccacatact ccgcgtcggt gatggacgaa
    11041 ccgagccccg ctccctgctg gacgaagact tcgtgaccgt gggcggtcaa ctcggccacc
    11101 cccaccgggg tgatcgccac ccggtactcg tggttcttga cttcgctggg aacggcgatg
    11161 cgcatcgggg tcctctctgg actagccggc ctgctgaccg gcacagggca cagtgtccta
    11221 cgtcgatggc ccactcgact atcgcggcgt gccgggaatc tgcgcgcaag tactagaatg
    11281 agggcaaacg tgctccgggg tcggtgtaat tccgaaccgg cggtgatagt ccgcgacccg
    11341 gttccgccca gcggaatcgg ttgatctggt ggaattccag ggccgacggt gaaagtccgg
    11401 atgggaggca gcacgcgtgt cgcgacgcga ccctattcag ggtcgtcggc gcggaccgtc
    11461 cgcgcatccc gaaaaccgcc ccgcagcacc tgaactgcgt gcggaggtcg gatgaaacgc
    11521 gaggtgctag ccccgatcgg gctgggcgca ctgctgctca tcggctggaa gctgaccgca
    11581 ggcggtggct ccgtgctgct gcccactccc ggtgcggtgg ccgcccgatt ggtcaccgac
    11641 ctgaccgatc cgcggtactg gggctacctg ctggtcacgc tcgccgaggc tttcggtggt
    11701 gcgctgctcg gggctgcggt ggccctgccg acggcgatcg gcatccaccg gtcgcgctgg
    11761 gccgcggcgg ccgtccatcc tttccttggt gccacccagg ccatccccgc gatcgcgctc
    11821 gcgccgctcc tggtgctctg gctcgactac ggtctgttct cgatcgtggt gctctgcgcg
    11881 ctgatcgtgt tcttcccgat cctgatcgcc gcggtcatcg gtctgaagca tgtggacgcc
    11941 gacgtggtcg acgccggccg gatcgacggc gcctccagtc tcgcgctgct gtacttcgtt
    12001 gaactaccga tggcgttgcc aagcatcctt ggcgggctgc gcaatggagt gacccttgcg
    12061 gtcaccggcg cgatcgtcgg cgagatggtg atgggcggca gcgggctcgg ccaggtactc
    12121 accatccagc gggggtcggt cgataccgcc ggcatgttcg ccacgatcat cgtgctctgc
    12181 ggcttcgcgt ccagcgccta cgccctgatc ctgctgtggg agcgccgctc ccgcatcgtc
    12241 aactccctgc agagagcctg aggtccatgc ccagaagaat ccttgccggt ctggtcgcgc
    12301 tattggcgct gagcgcctgt gcgccagcca ctcagcagag ctcggtttcg gccaccgcgg
    12361 tggcgaccgc gaccccgacc atcgggctca cctacatccc gaacgtgcag ttcagcccgt
    12421 tctacctggc ggatgcggac ggtgatttcg cgcgggccgg gctggctccg accctgcggc
    12481 accacggggc gtccgagggg ttgttcaccg ccatcgcagc tggtcaggag cagttcgtga
    12541 tcgcaggtgg cgacgagatg cttcaggcaa gggcgcaggg gctggacctg atcgctgtgg
    12601 gggcgtactt ccacgagtac ccggcggtgg tgatcgtccc cgaggcatcc gcgatcggct
    12661 cgctggccga cttgaagggg cattcgatcg gcatcccggg caagtacggc gagaactggt
    12721 tcgcgctgct ggtggcgctg cggaccgccg ggctgaccga ggccgacgtg gagatcaagg
    12781 agatcggcta cacccagcag gccgcactgt cccagggcaa ggtggacgcg atcgtcggct
    12841 tcagcaacaa cgaagcggtg cagttcgcgc tcggcgggct ggcgacgcgc agccttgcga
    12901 tcgcggacgg gcaggtgcct ctggtcagca tctgtctgat caccacctcc gagtacgcga
    12961 aagccaatcc ggacgtggtt cgtcgggtgg caaccgcgat gctgggcggg gtcaagaccg
    13021 cggtgaccga tccggaccac gcgctggcgg cgtcagcgaa gttcgtgccc ggcctgtcgg
    13081 cgtccgctgc ccaggcggcg gcgaaggcca cattggtcgc aaccgcgaag ctctgggtgg
    13141 acgcgtccgg tgaggtctcc gggaagttgg atcccgacca atggacggcg atggccgctt
    13201 tcatgaccga tcacggcttg ttgaccggcg atgcggacgc cgctgccgcg atgagcaacg
    13261 agtatttgcc caagtaatca gcgggctgcc agctcacccg gtagcctgct ggcgcatcgt
    13321 ggccgccgca gacaggagaa ccgatgaacg agcactacgt gcccgggtgg gacgagtact
    13381 tcctcggtat cgccagcgcg gtggccgcac gcgcgaagtg cacgcgccgt cgggtggggg
    13441 cggtgctggt gcacgatcgc cggatcatcg ccaccggcta caacggtgct gcgcccgggc
    13501 gtccggattg ccttgagggg gcctgcccgc gcggtcagct cgactacgac caggttcccg
    13561 ggctgggcga ctacgaccgt ccggggacgc cgggtttctg catcgccatc cacgccgagg
    13621 tgaacgcgct gctcttcgcc acccgcgata ccaagggtgc gaccgcctac atcaccgacc
    13681 cgccgtgccc cggttgccgc aaggctctgg ccgcagccgg catcgtccgc gcggtctggc
    13741 cagatggaga gcacgacacc gacggcttgg tgaactggga cgcctgagct tcggcctcac
    13801 ctgcgcttgg gtggagctat cagtaccagc cgtggccgcg cttgaaggac caggcctggc
    13861 agggggtgcc gtagcgctcg tcgacgtaat tgaggcccca ccggatctgc gtggcgggat
    13921 tcgtccgcca gtcgtcgccg aactcggcca tgcgcttgcc gggcagtgcc tgcggaatgc
    13981 cgtaggcact ggagtacggg ttgtccgcgg tcacgatcca gcggctctcg cgcatgatga
    14041 tgctgtcgta gcaggcgaac tggtcttcgc cccactcgta cttgttcagc atgatctgcc
    14101 gggcgatctc gcgcgggtcg gtggtggcgg gttcgtagcc gagttcggcc accgtctggg
    14161 cgtggatcgc ctcgatcgtc gcgttctgga cggcgatcgc agtcgactgc ttgttcagca
    14221 gggcctcgcg atagatcgcc aactcggccg gcttcaaggt cgtcgtgcgg gtggcggctg
    14281 gcggattggc ggagccggcg gaatcagcgg agggaaccgc cagagcctgg ctcgcggtga
    14341 acgcgggcag cagcccgatc actgccgaca gcgcggccac tccgttgcgc agccgtcgga
    14401 tgttcacgcg cggagtcttt cataagagac cctctcaggt aaaactcagc ttgtgtttgg
    14461 ggctcagcca gcgtgcgcgt cgttggtcct atggttgagg cgtgcaactg cgggtggtcg
    14521 aaggcgcgga cgtcgtcggc gcgttggccg atgcgttggc cggtggaccg ccgttggcac
    14581 cgcttcccga ggcgcggcaa ctacccgccc tccagatcga cgagccggtc accgaaccgg
    14641 acgccggcgc ggtcgtcacc acctcgggcg cgaccggagc acccaaggcg gtggtgctgt
    14701 cccggtccgc gctgcgcttc tccgcggacg ccacccacgc ccggctcggc ggcgccgggg
    14761 attgggtctg cgcgttaccc acccgccacg tcgccgggtt gatgaccgtg gttcgcgcgg
    14821 tccgggccgg gacggcggtg cacttcgccc gtccggacct cgccgacctg gaaacaccgc
    14881 gaacccgtac ctacatctcc ctggtcgccg cccagctcga ccgggcactc cacgaagccg
    14941 aaacagtcgc caagctgggg gccttcgccg ccgttctgct cggcggcagc gcgcttccgt
    15001 ccggcctgcg cgaacgcgcc aacgacgccg gcgtgaaact agtggccacc tacggcatga
    15061 gcgagacctg cggcggctgc gtgtatgacg gcgtcccgct ggacggcgtc cgggtcgaac
    15121 tcgaaggcga acggatcagc ctcggcggcc cgatggcctt tctcggctac cgcggccgtc
    15181 ccgagctcac ccggacggtg ctcagcggcg acctggtgcg caccagcgat cggggcatct
    15241 gggcggacgg acgcctccgc gtgctcggcc gcgcagatga cgtggtcatc tcgggcgggg
    15301 agaacgtcga cctcgccgca gtccagcacg cctgcgacgc cgagttcggc accggaacgc
    15361 tggcggtcct ggccatcccg gacgctcggt ggggcgcgaa ggtcgtcgca ctcagccgtg
    15421 ccgacctcga ccttggccgc gtccaagcca tcctggagcc gcggctgggc aaggcggcgg
    15481 cgccaaagga attgcggcga gtcgagcact tggcgtacac atccataggc aagatcgatc
    15541 ggacggcact ccagctgtcc tggcgggagt tgggtgaaga tggcgacgtt ggctgaatgg
    15601 gtcgagggcg cccgcccccg caccctgccc gcggcgctcg cgccagtgct cgcgggtagc
    15661 gcggtggcct ggttcgaggg tgggttcgac ccgctgctgg cgacgctcgc actggtcgtc
    15721 gcgctggcgc tgcaggtcgg ggtcaactac gccaacgact actccgacgg catccgcggt
    15781 acggacgcgg tgcgggtcgg cccgatgcgc ctggtcggtt ccggtgcggc caagcctgcg
    15841 cacgtgaagc tggcggcctt cgcgtgcttc ggcgtcgcgg ccgtggccgg cctgaccgtc
    15901 gtggtgatca ccggccactg gtggctgctg ggtctgggtg cagcggcgat cctggcagcc
    15961 tggttctaca ccggcggccg caagccctac ggctacctcg gtctcggcga actgttcgtc
    16021 ttcgtcttct tcggcctggt ggcggtctcc ggcaccgtct acatccaggt cgggacggtc
    16081 tccgcctcgg cttggtggac ggcggtggcg atcggtgcgc tggcctgcgc gatccttgtg
    16141 gcaaacaacc tgcgcgatct ggacggcgac cgtgcggtcg gcaagcggac gctcgcgact
    16201 cgtctcggcg acagcggcac ccgctggttc ttcgtcgggc tggtcgcgct ggctgcggtc
    16261 ggcgtgtttg gcgtggccgc gaccagttcg tggtgggcgc tgctgggctg gctgatgctg
    16321 ctgctcctgg ccggcccgat gctggaggtc gtccgtgggg tgagcggcta cggcctgatc
    16381 cacgtgctga agcggacggg gctggccgag ctcgcctgcg ccgctgggct cttcgtcggc
    16441 ctcctgatcg cgcgctgacc gatgggacgc aactccgcgg tggcgttcgc gctcccgatg
    16501 cgcaaccggt tccgcggcct taccgtccgc gagggcctac tggtgcacgg cccggccggg
    16561 tggggggagt ggagcccgtt cgccgagtac gagcgtccgg agatcgacaa ctggtggcgg
    16621 gccacgcttg aagcggcgaa cctcggtttc cccgagccgg tgcgcgaccg ggtgccggtg
    16681 aacgcgaccg ttcctgcggt cgaccccgag acggcctacg cgatcgtcgc cggatcgggt
    16741 tgcggcacgg cgaaggtgaa agtggccgag cccggccagt ccggcaccga ggacgcctcg
    16801 cggctggagg ccgtgcggga tgcccttggc ccgtccgggc gactgcggat cgatgccaac
    16861 ggcgcttggg acgtggacac cgcgctcagc cgattggcgg agttggccca gttcgggctc
    16921 gagtacgccg aacagccctg tgccacggtc gacgaactgg ccgagttgcg ccggctgctg
    16981 gcccggcgcg gcttccaggt gccgatcgcg gcggacgaga gcatccgccg cagcggcgac
    17041 ccggagcggg tggtcgcact cgaagccgca gacatcgcgg tgttgaaggt gcagccgctg
    17101 ggcggcgtcc gcgcgtgcct ggaactcgct gagcggttgg gcctaccggt ggtggtctcg
    17161 tcggcgctgg aaagctcggt ggggctcgcc gccggcgttg cgctggccgc cgcactgccg
    17221 gagttgccct acgcgtgcgg actgaacacc gccacgatgt tcaccatgga tgtcaccgac
    17281 cgcccactgg tcgccgtcga tggcgcgatt cccgtgcgga cggtctcgcc cgatcgtcat
    17341 ccctgcgcgg ccccaaccac cgctgatcgt ggtcccggcg cggccctttc agccaccgct
    17401 gatcgtcatc tgggcgttgg ccgggatctc ggttcgcaac cggtggtcgg gggggcacag
    17461 gacgttcgtg ccgacgactg gcgagccggg ccggaagcga cggcccgctg gctggcccgg
    17521 ctcggcccaa tggctgagga actgatcgaa ccaggggagg gccgatgacc gactccgtcg
    17581 cctgcgccag cgcggtgctc gggcagttgc tggcccaagg tgtgaccgat ctggtgctcg
    17641 cccccggatc gcggagcgcg ccgctcgccc tggtggggga cgcggccgcg cgcgcgggcc
    17701 ggttgcggct gcacgtccgg gtggacgagc ggtcggccgg gttccttgcg ctcgggctcg
    17761 cgaaggcgtc cggacgctgc gtcccgatcg tgaccacgtc cggcaccgca gtagggaacc
    17821 tgctgccggc ggtgatggag gcacaccatt ccggggtgcc gctgctggtg gtatccgccg
    17881 accggcccgc cgcgctggtc ggtttcggcg ccaaccagac caccgagcag agccaacttt
    17941 tcgatgggtt cctccgctac cgcgcccggg tcagctcggc cgcttcggcc gtttcctggc
    18001 ccgcgcaggc tgcgcgtgca gtactggccg caaccggcgc gctctcccgg ttccccggcc
    18061 cggcccagtt gaacgtcgag ctcggtcagc cgctggtctc ggatgcggtc gaggcgccgg
    18121 ccgagttcag accggtactc accccagacc agccggacgc agtggtgcat ccgctccctg
    18181 gggccccgcg caccctggtg gtctgtggcg atgcgccggt atcaatcgga cgcgtggccg
    18241 tcgagctggc cgaggctgcc ggcctgccct tgctggccga accgtcgagc aacgcgcgca
    18301 ccggtgcgaa cgcgctcggg cacggccgaa tcctgctcgc gggcccgctg gccgaacaga
    18361 tcgagcgggt gatcgtcttc gggcggccca acctgtcgcg tccggtgacc gcactgcttt
    18421 cccgtgccga catcgaggtg atcgtactca gcgcgtcccc ggacttcgtc gacccgggct
    18481 ggcgggcgag cgtggtcgcc ggcgcggtca gcgtcgcacc cggcccgccg gattggctgg
    18541 ctgcctggca ggacgccgat tctcggctgc gtccggttgc gctgggagat cccggcctgc
    18601 gccgggatga cggtgggttg gggttgcgcc gggatgacgg tgggttgnnn nnnnnnnnnn
    18661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18721 nnnnnnnnnn nnnnnnnnnn nnnnnnnccg ggccgcaggt cgcggcgctg gtggtcgcgt
    18781 ccgtgccggc ggatcaggtg ttgtgcctgg ggaactccaa tccgatccgg gacgctgatc
    18841 tcgcgccggt gcgtccggac tccccgccgg tcttcgcgaa ccgcggcctg tccggcatcg
    18901 acggcaccct ggccaccgcc atcggggtcg cgcttggctc gggacggccg accacgctgc
    18961 tgtgcggcga cctgtccttc gtccacgacg tcggtgcgct ggcgatcgga ccgggggagc
    19021 cgcgtccgga actgcgcatc gtggtggccg atgatgccgg tggcagcatc ttcgcgaccc
    19081 tcgaatacgg cggcgaacag ttcgccgact cgttcgaacg ggtcttcgct acccccaccg
    19141 gcctcgacct ggtcgctctg accaggggct tcgggatacc gtgcgtccgc atcagcaccg
    19201 cggacgaact cgcgtccgcc ctcgacgaac cggtccgcgg actggaagtg ctggtggtcg
    19261 acgtcgatcg cagcggacgc cgcgagccgg ccccagccta ggctcgaggg cgcccgccgt
    19321 ccgacttcgc tcccgaatgg gactcgcgct cccgaactat cgggagcaag ggtcccgttc
    19381 gggagcgaag ttgccgaagt tggcggacgc gggcaccgcg ccagccgtca cccgagcaac
    19441 ccaaggcaga atgtcaggac attgtgttga cttctgctgt tagcagggat tgaatgggtg
    19501 tcaccgactg gtagcggcac accgtgcctc gaagaagagg ttcaaatgac tgatacgaac
    19561 accccacttg gcctgatgac tcagcgcggc ccgaccgtgc tctggaacga ctcggccacc
    19621 tacaccgagc tttccacggc catctcgtgg ggcgcggtcg gcgccacctg caatccggtg
    19681 atcgcgctcg ccgcgatccg cgccgacctg ccgcgctgga ccgcccgaat caaggaactc
    19741 gccgccgaga tgccggacgc gaccgagtcg cagatcggct ggaaggtcgt cgaggaggta
    19801 tccctcgatg ccgccaagct gctggagccg gccttcgaaa agtaccaggg ctacaacggc
    19861 cggctgtcga tgcagaccga cccgcgcctg gctcgcagcg ccaaggcgct ggctgaccag
    19921 gccgagtact tctcgaacct cgcgccgaac atcatcgtga agatcccggc gaccaagacc
    19981 ggcgtcgagg ccatcgagga cgctacccgc cgcggggtca acatcaacgt caccgtctcc
    20041 ttcaccgtcg cccaggcggt agccgccggc gaggccatcg agcgcggact gaaggcccgc
    20101 gaggccgcgg gggaggacgt gtccaagatg ggcccggtgg tcacgatcat ggtcggccgc
    20161 ctcgacgact ggctgaagca ggtctacgcc aaggacggcc tggtcttcga cccgggctac
    20221 ctggagtggg ccggtgtcgc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20341 acccgcaaga cgctgcgcca gttcctcggc gccgacgaag aactcgacca gatcgtgcgt
    20401 gacatcctga tccctgcagc ctgattcccc tcgcgccgcc ggcgtcgggc caccctttcc
    20461 ccacgccggc ggtgcaccga ctcgaacacc aatgacccgg agccaaagat gacccagacc
    20521 gttcggctaa ccgtcgccca ggcggtggtg aagttccttg ccaaccagta caccgagcgg
    20581 gacggggagg agcagaagct cttcgccggc tgcttcggca tcttcggcca cggcaatgtc
    20641 gccggcgtcg gccaagcact gctgcaggct gagctccagc aactggacgg caacccggac
    20701 gcgcccgacc tgccttacgt gctggtgcgc aacgagcagg gcgccgtcca cgccgctgcg
    20761 gcctacgcca agaccaagaa ccggctgcag acctgggcgg tcacctcgtc catcggcccc
    20821 ggcgccacca acatggttac cggcgcggcg ctggccacca ccaaccggct tccggtgctg
    20881 ctgctcccgt ccgacatctt cgcgacccgc taccccgacc cggtgctgca gcaactggag
    20941 gacccgcgca ccctcgacat ctcggtcaac gacgccttca agccggtctc gcgctactgg
    21001 gatcggatca accgtcccga gcagctgatc ggttccctgc tggcggcgat gcgggttttg
    21061 accgaccccg ccgagactgg cgcggcgacc ctttgcctgc cgcaggacgt gcaggctgag
    21121 gctttcgact tcccggtgga gctgttcggc aagcgggtct ggcacgtcgc gcgtccggtg
    21181 cccgaaccag ccgcgctggc ccgtgcggtc gcggcgatca aggcggcgaa gcgtccgctc
    21241 gtggtcgctg gcggcggcgt gatctactcc ggcggctcgg acgccctgcg tgcattcgcg
    21301 gacgcgaccg gcatcccggt ctccgacacc caagccggca agggcgcaat caacttcgac
    21361 caccggatgg cggtcggcgg cgtcggctcc accggcggca gcgcggccaa cacgctggcc
    21421 cgcgaggccg acctggtgat cggtatcggc actcgttact ccgacttcac gaccgcgtcc
    21481 cacacgatct tcgccgaccc gaacgtgaag ttcgtcaacg tgaacgtgct ggccttcgac
    21541 gcagccaagc atgccgcgac catggtggtg gcggacgcga agtcggcgct cgaggcgctc
    21601 accgcagaac tctccggctg gtccaccgat gccgcctacc aggcgcgcgc cgagcagctg
    21661 gacgccgaat ggcaggccgt cgtcgacgag tgctaccacc gcgacaacca gccgctgccc
    21721 gcgcagaccg aggtcttcgg cgcgctgaac gagctgatgg gcgagaagga cgtcgtgatc
    21781 aacgcggccg gctcgatgcc cggtgacctg caggccctgt ggcgtgcccg cacccccgag
    21841 caataccacc tggagtacgc gtactcctgc atgggctatg agattcccgc cgccctcggg
    21901 gtgaagctcg cggtcggtga cgagcacgaa gtggtcgcga tcgtgggtga cggcacctac
    21961 cagatgctgc cgatggagat cgccaccatg gcgcaggagg gcatcaaggt gatcctggtg
    22021 ctgctgcaga accacgggtt cgcgtccatc ggtgcgttga gcgagtcgcg cggttcgcag
    22081 cggttcggca ccaagtacaa gacccgctcg acgtcgggcc gactggacgg ggcggatgtc
    22141 cccttcgacc tggccgccaa cgccgccagc tggggcgccg acgtgatccg ctgcaacggg
    22201 atcgccgagt tccgcgagaa ctaccccaag gccgtggcga acgaccgcac gaccgtcctc
    22261 tacatcgaga ccaacctgta cgggccgaat ccgcccggaa ccgcttggtg ggacgtgccg
    22321 gtatcacagg tctccgagtt ggcgagcacc cagcaggcct acggcgagta catcgccgag
    22381 aagtctgcgc agcgccatta tctgtaggtg aatgctctct cgaacctctg ggccagcctg
    22441 ccgctttggg gctggctggc cctggtgctg tgtgcgctgc tgatcggctt cgccaagacc
    22501 gcgatcggcg gcgtggcgat gctggcagtg gtgctggcgg cgatcgtcct gccggccaag
    22561 gactcaaccg gcgtggtgct ggtgatgctg ctggtcggcg acttggtggc ggtctgggcc
    22621 taccggcaca acgtcgactg gaagttgatc ggacgcctcg tgctgcccgt gctggtgggc
    22681 atcgcgctgg gggcgtggtt tctgaactgg gtggacggac caacgctgaa gcggaccatc
    22741 ggcgtgatcc tgctgctcct gctggtgctg ggcctgtttc gaaatcggct ggcggtgcac
    22801 caccctgcgg tcggggcggc gtacggcggg ctggccggct tcaccacgat ggtggccaac
    22861 gccggcgggc cgccgatgaa cctgtacctc ctggccgcca agtacgacaa gtggcgcttc
    22921 ctcggcacca ccgcctggtt cttcttcgcg gtgaacctga ccaagctgcc ggtctcgatc
    22981 agccagtcga tcgtgcg
//
LOCUS       DAOPRQ010000041        22623 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_1896_length_22623_cov_11.762318, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000041 DAOPRQ010000000
VERSION     DAOPRQ010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 22623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22623
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1896_length_22623_cov_11.762318"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(221..2293)
                     /locus_tag="PKJ61_06885"
     CDS             complement(221..2293)
                     /locus_tag="PKJ61_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012919210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HOC13586.1"
                     /translation="MADEGRATKTTSQSRPRRSWRLPADREPERPDYAVVRRLILGSA
                     LMLFLELALIRWLGSNVVHLSYFTNFVLLGSFLGIGLGFLIARYTWSVLPATPVILAV
                     LVIATFLFPVTIDRAGDQIIYFTSLSTTGPPAWLVLPLIFILVATVLAGPAEVVGRCF
                     ARLRPLTAYRWDLVGSLIGIGSFTLLSFLQAPSVVWGVLVTGAFVVLIGGWQRYLAAV
                     AGAAMVVALLLETLTPGVSWSPYYKVQTKELTSAGVPLTDISVNGVPHQIMRAASQRL
                     QEEPQYGLPYERTPGNPLRNVLIIGAGSGSDVAITLAKGAQHVDAVDIDPRIMQLGME
                     KHPDHPYDNPLVTRHVNDGRAYLEATDTKYDLILFALPDSLALVTGASQIRLESFLFT
                     EQALTAAKEHLTPEGAFAMYNYYREDWLIDRLAGTAEAAFGHVPCVDLVGNGQAVVSA
                     ALDESKQSCGTPYSPAGLVVAPATDDRPFLYYQGGPIPPLYLWTLGGILLISVIAVRV
                     LGGPFKEMRTYADLFFMGAAFMLLETKNIATFALLFGTTWLVNALVFAGVLVIVLAAV
                     ETTRRFRTPPLPVVFGGIAASLAVTYFVEPDWLLTLPFAPRLIVAILLAFVPIYLANV
                     AFSKRFGASDDSRSAFGLNLLGAMLGGCLEYFALLTGYRNLLVMVAILYLLAFLLTPR
                     TKGALVSV"
     gene            2433..3020
                     /locus_tag="PKJ61_06890"
     CDS             2433..3020
                     /locus_tag="PKJ61_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007077811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOC13587.1"
                     /translation="MIVRPMLARAYWALSRYRLQSEPPPDTSAVLIGAPHTSNWDFVI
                     MLAISWQHGVAPRWLGKHTLFKAPFGRLMRALGGIPVNRANPGRLVAEVVELAQAEER
                     VFIVVTPEGTRHRGKLWKSGFYRIARDANLPLVLGYVDSATRTAGLGPTMHLSGDVVA
                     DMDQLRAFYADKHGIRPEDFTPPRLREEGSAESPK"
     gene            3103..3678
                     /locus_tag="PKJ61_06895"
     CDS             3103..3678
                     /locus_tag="PKJ61_06895"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase activity [Evidence
                     IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HOC13588.1"
                     /translation="MDQYQVGYFVGSLSSKSINRVLATALIKLAPAELSFTEIEIGNL
                     PLYSPDFDADYPPEGRALKDAIASTDAVLFVTPEYNRSIPGALKNAIDWASRPWGENS
                     FNHIPAAVIGASPGAIGTALAQQSLRGVLSYCNARQMTAPEAYLKYDPEIFSADGEVH
                     DESVKQFLTDFMGYFHVHLSRVLTVLPRSTT"
     gene            complement(3682..4512)
                     /locus_tag="PKJ61_06900"
     CDS             complement(3682..4512)
                     /locus_tag="PKJ61_06900"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HOC13589.1"
                     /translation="MNLFPWTTVSSGETSERVSAIQYLLRAHGHQLNVDGAYGPATVK
                     AVKALQAASGLTTDGVVGPKTWPKLVKTISIGSTGDAVLALQVGGLATPPWDGPLDVD
                     GVFGPETKERVEGFQYNWGLTRDGAVGPETWSFATTEDGPWPLVKVGATQESNWRVRS
                     VQHLLRAHGASIVADGSYGPLSGEAMKFFQLGLRATEISTTCGQLDWPELALTVSPGD
                     SGEAVRAVQWLLPDLTTDGLFGPKTESAVRAFQDKFLPTTDAIVGPMTWWALVVPAYG
                     "
     gene            4738..4965
                     /locus_tag="PKJ61_06905"
     CDS             4738..4965
                     /locus_tag="PKJ61_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13590.1"
                     /translation="MSGGTAIRTPDQLLRVFVSSTLGELAPEIFDAAQARGRELTTKE
                     AVALALSTEQSAKVTELKPGRPAFPEVAVAS"
     gene            complement(5035..6900)
                     /gene="recQ"
                     /locus_tag="PKJ61_06910"
     CDS             complement(5035..6900)
                     /gene="recQ"
                     /locus_tag="PKJ61_06910"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020387346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HOC13591.1"
                     /translation="MPVASADPLEVLRSTFGYAAFRGDQGAIVDQVVRGGDAVVLMPT
                     GGGKSLCYQIPAIVRPGMGVVISPLIALMHDQVTALDALGVRAGFWNSTLSAEERRAT
                     ERAAIAGELDLLYLAPERLGTEWAQQFLDRLQISVFAIDEAHCVSQWGHDFRPDYLRL
                     SVLHERWPDVPRIALTATATPATRQEITERLALQDARTFVASFDRPNIQYRIEPKQQP
                     LRQLVSLIRDEHDGDAGIVYCLSRASVEKTATALVKEGITALPYHAGLPTNVRSANQA
                     RFLREDGVVMVATIAFGMGIDKPDVRFVAHLDLPKSIEGYYQETGRAGRDGLPATAWM
                     AYGLADVVQQRQLIEGSDGSAQHKRLLGQHLDSMLGLCETLRCRRQLLLAYFGEASEP
                     CGNCDTCLAPPQAWDGTVAAQKLLSTIVRLQREHGQRFGAGQLIDILLGRSTERVQRF
                     GHERLSTFGIGTELGDQQWRAVTRQLLAAGLLAVSADGHGTLGLTEASWPVLRGEQPV
                     PLRQDAVARAQRSGRRAAPKGGSRSAAASELSGEDRELFEALRAWRGETARQASVPAY
                     VVFPDSTLAGMAQLRPDSLDALAGVSGVGAKKLEQYGEAVLRIVGQPIPELDEGP"
     gene            7128..8618
                     /locus_tag="PKJ61_06915"
     CDS             7128..8618
                     /locus_tag="PKJ61_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016669934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HOC13592.1"
                     /translation="MYLGVDFGTTRTIVVRADRGNYPVVGFVDPAGDTAEFFPSVIAL
                     ADTQPMYGFAAQVAARNGAPHLRSIKRLLGQPGVTATTGVRLGERTYPLLELLTGFLT
                     ELRRQLVEHSDLTGELTARTALPTLVSVPAHAHGAQRFLTLEAFRGAGFDVTGLVNEP
                     SAAGFEYTHRLGGTLTSRRDRVLVYDLGGGTFDASLLAVAGSSHDVIGSVGVNRLGGD
                     DFDTALAELALEASGRPELDEADYGALLAECQEAKERLSPQSRRLTVEVAGQPVLVSV
                     ADFYAAVTPLVNSSIEAMAPLVAALDADAALAQVAGLYLVGGGSGLPLVPRLLRERFG
                     RRVHRSPYPAASTAIGLAIAADPDSGFSLADRLWRGFGVFRELDAGRAVGFDQLLDRH
                     TELPRSGELTIHRRYRAAHNLGWYRFVEYADVVAGEPRGDLAPFAEVRFPFDPALHGR
                     DLTSITIERRPDGPLVEEAYSLDANGIVTVRITDTATGYTQTHALG"
     gene            complement(8636..9508)
                     /locus_tag="PKJ61_06920"
     CDS             complement(8636..9508)
                     /locus_tag="PKJ61_06920"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HOC13593.1"
                     /translation="MDAFAPVGSARRLEILHQTRLGYSDDVSASFTEARMTPLSENGQ
                     VLLAHKVRVKPSASLSRHVDYWGAQVTTIELHSPHRELDIRAASTVLVPGARELGEPV
                     GWDDLRDPGFVDANCEFLGFTDLADAELPVGVAADLVADLATAPNPRAAVLGCVRAVR
                     ERIAYEPGITSVTTTASEAWTAGRGVCQDFTHATLAILRALGIPARYISGYLHTEEPS
                     PGRTVVGESHAWVECWDGGWTAVDPTNDRRVGAAHVLVARGRDYADVPPLSGVFAGGP
                     SSGQSVEVQITQAE"
     gene            complement(9508..10413)
                     /locus_tag="PKJ61_06925"
     CDS             complement(9508..10413)
                     /locus_tag="PKJ61_06925"
                     /inference="COORDINATES: protein motif:HMM:NF016086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HOC13594.1"
                     /translation="MLSRIAESFYWMGRYLERAEATTRLLAEHHQLVVEDQSVEFSLA
                     AGVLVDALAISSARPTTGEHLARLVIGSPSEPATISGAVSAARDNARSVREALSSDTY
                     ESINAVQILLARGFGRGTSPGVALHRLLERLLVVNGVVEWTMPRDEAHDFLVLGRELE
                     RIDMMGRMLAMRHDLLWPTIGPAAVLRACGGLSAFLRTGEPSVGEKVRAFLVLDRTFP
                     RSMRACADDAEQAVRALASSGVSDGGQLLSEVGMLRSNLEYASAGQPGVVDALIETAA
                     AAGARAADLVNAAYFRQHGTIVWSH"
     gene            complement(10407..12038)
                     /locus_tag="PKJ61_06930"
     CDS             complement(10407..12038)
                     /locus_tag="PKJ61_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015070880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HOC13595.1"
                     /translation="MTAVTSTPAAFDEMLDAGGPRAADEPLLAALERLGPDGRAERAR
                     QRDAYLDRHGITFTLGERERPLPMDLVPRLITASDWAVIEAGVAQRLHALEAFLADVY
                     GPGMIFQDRVIPRSLVTTSTHFARQAWGLRPANGVRIHVSGIDLIRDEAGVFRVLEDN
                     LRNPSGVSYVLENRRTLAHVLPEIFADYRVRTVGEYPEHLLDALRAAAPTAGSQPTVV
                     VLTPGVHNSAHYEHAFLARHMGIELVEGRDLYCRDSKVWMRTTSGPRQVDMIYRRIDD
                     DYLDPLVMRPDSVLGVPGLLNAVRANTVGIGNFVGNGVADDKAIYPYVPAFIRYYLGE
                     EPILENVPTYDLTDVDQREHVIANIDRMVVKPADASGGYGLLMGPSASEDQIRATIAA
                     LEANPRGWIAQPVVRLSTCPTVVGDDGEMASRHVDLRPFATNDGDRVRVLPGGLTRVA
                     LVAGSLVVNSSQGGGSKDTWVLEDSAAPQVEAVPVVIRVAPVSAWLPARPNAVPSHND
                     RLAQQERQQQQAAQPDVEPEAGAATDATGERLAEC"
     gene            12339..13658
                     /locus_tag="PKJ61_06935"
     CDS             12339..13658
                     /locus_tag="PKJ61_06935"
                     /inference="COORDINATES: protein motif:HMM:NF013357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HOC13596.1"
                     /translation="MSAPSQPALKLLIAAATAVVVTVGLMPSNADARPVAPGPAAAPA
                     ALPLAEPLPGPVITVDLVDAVIGRYGTRTSATFKVEATGEKLRYQWQRLAPYAKKWKN
                     VSGAKKAKYTAKATTWASGTSFRVVVTGTGGKVTSAPAKLTVLYPTKSPAKDAEAAFG
                     LTGLRQGVDLSAYQWTPAAKIRMKKVDAWAGKDGFTILRNGSGSRPIKQQYTDACTNK
                     ARKTGTEPVTEDCAYAKLAKQAKSAGLSLGHYWFNGWIASTDSTTQQLFAGGYSATAS
                     AEQFVKWLKRDGRYTKSSTDPLVLDIEAGNAWTKAKDGEKIKQKLRAWNPSEAAEFLT
                     AVKQLLTADGYQANLYVYMSANRTLQLGPTGYLWQDVAGIAKLWVASWGTNNGRIPDA
                     QPEVGPWAEAGGWSIWQYTSNARFSGSGVGALDGDIAKADAWTPLAG"
     gene            complement(13755..14456)
                     /locus_tag="PKJ61_06940"
     CDS             complement(13755..14456)
                     /locus_tag="PKJ61_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002855489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13597.1"
                     /translation="MWSALGWGALAASSVVIGALLGLVRSWHRGAVGMVLAFGAGALI
                     SSVAYELVEDGLRTSGPAPIGIGLALGALVFYLADRAVERLNSGASSGLPLALGALLD
                     GVPEQLVLGLGLASGGGISLALFASIFVSNLPEGLGSAAEMRDAGRSRRSIFGLWTVV
                     AVCSTLATLAGFGLAEVLPAAVGAGVNGFAAGALLVMLTDSMIPEAQSKASGKAGLAT
                     VLGFAMAMSLSLLQP"
     gene            14505..14735
                     /locus_tag="PKJ61_06945"
     CDS             14505..14735
                     /locus_tag="PKJ61_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13598.1"
                     /translation="MTSDYRFTTEEDAVDHPLVHRWLSEQSYWANGRSRDTLDAAIDG
                     SRNYSVLEVGLYRQFGFTALAEPGRWMVRGRS"
     gene            complement(14803..15168)
                     /locus_tag="PKJ61_06950"
     CDS             complement(14803..15168)
                     /locus_tag="PKJ61_06950"
                     /inference="COORDINATES: protein motif:HMM:NF018663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOC13599.1"
                     /translation="MKRLASSPYPQPTGPAQPGNLMYADFQILDQWFAVMDSGVEQDF
                     TFNCGVSLMVECADQAELDRYWNQLSAVPEAEQCGWCQDKFGVAWQFIPANLGELMTK
                     PDAYRKLMNMKKIEVAAFD"
     gene            15259..15627
                     /locus_tag="PKJ61_06955"
     CDS             15259..15627
                     /locus_tag="PKJ61_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13600.1"
                     /translation="MLVKLYQPGGSKAAKSAKVAQTDDQRVRTAARDYLNLRKSHAGL
                     LARGDASPVALAPASGAVGSSEAEAGDDGTTVVYAKSNRSYQGILGGAGAIIGGALGG
                     APGAAIGGAIGGVIGMIVDD"
     gene            complement(15650..15904)
                     /locus_tag="PKJ61_06960"
     CDS             complement(15650..15904)
                     /locus_tag="PKJ61_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13601.1"
                     /translation="MYEPSDRVGELKAQAADARVRVIESNKLVIAELSRIWHELNETA
                     AGQPEVRKHCEHVAADIARLEKLNLAFDTEWLAEAKRDRP"
     gene            16020..16385
                     /locus_tag="PKJ61_06965"
     CDS             16020..16385
                     /locus_tag="PKJ61_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008355407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13602.1"
                     /translation="MTAERRRSAAGKNTEADVLAAIEAIDTNVRPMAEGLHALVKREL
                     PELTSKTWYGFPAYCKGGQVVFFFQYAGKFKSRYGHIGFQDTAALDDGVMWPTAFAVT
                     EWNADVEARVAELIKRAVS"
     gene            complement(16410..16994)
                     /locus_tag="PKJ61_06970"
     CDS             complement(16410..16994)
                     /locus_tag="PKJ61_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012924032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOC13603.1"
                     /translation="MTQVTSEPVSGLRERKKSATRTAIHETALRLVAERGPQSVTVEE
                     ICAEVGVSPRTFFNYYPSKVAAAFDLLVTDIPADQVEWFLNADGSLVADTCDLIGRNV
                     TLPTDFPRIKALMHGQPELLMDFWTQTIARLKPFFKLIEQRTGDAHTARIVFGIVLAA
                     VTAAMSRPDAAEAGDIAARLRAEIRTMRDLIAEV"
     gene            complement(16991..19633)
                     /locus_tag="PKJ61_06975"
     CDS             complement(16991..19633)
                     /locus_tag="PKJ61_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020574307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HOC13604.1"
                     /translation="MAVATVERTAPIEQTPNQPVMNHGQIMLVMYALMAGMFLSALDQ
                     TIVGTAIRTIGDELHGLDQQAWVTTAYLIAATVTTPLYGKLSDIFGRRPLFIISISLF
                     VAGSFLSSFSTSMIMLAGFRAFQGLGAGGLMSLPLAIIGDMLAPRERAKYQGFFLATF
                     GIASVIGPLVGGLFAGADEILFISGWRWVFLINVPVGVIALFMVVAFLHLPHHPRGRR
                     HIDWWGAGLVVATLAPLLLVAEQGREWGWTSVPAMICYAIGVASLTAFILVERAMGPD
                     AILPLRLFDNKSFSTATVLSILVGFGMFGAMLTLPLYLQIVLGFSPTESGFATLPMMA
                     GIMLASVGTGQIVARTGRYGAFPKTGTATTAVGFFLLTLMTLDKPYWYLAIAMFVIGL
                     GLGQLMQTLTMAVQAAVDAQDIGVATSSATFFRQIGGTLGTAIMLSLLFSILPTNISG
                     ALTDEHTTWHALDAALNPKVAGDPANAAIMDKLWTPITDKIKDQVDEKLADATEQVKD
                     GVETAVRAKVTEAVSKKAAKGTGKIADGINALGTGLDKLALGTDAFVDGVGKIGSGAQ
                     QLATGTSKIAKAENQLASGLAKLSDAEQTAAAKAKAAAGDYVTLSAAIRTMTADQDSC
                     VSGETAACDQLVADQAEVDAAMGSLQGNVYTTSGMLNGAAGQSGLAAGLASAAKGTSA
                     LADGLEQLASGTHKLADGATTAAAKGSAIPTGASAAASGSAKLASGAKQLSKVDQLID
                     KAVKKALPDAYAKALQQVAFERNLSVKDGVLAIDWSDPVQRWAMVDELTPTLVQTIKN
                     GDAAGSDVNTDSSTSDTSFLTGADPRLTQPFMVGFSDSVITIYWVGLGVMVLAFVVTL
                     FFKVPPLRTRSALQEKSDALEGLA"
     assembly_gap    19690..19789
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<19790..20226)
                     /locus_tag="PKJ61_06980"
     CDS             complement(<19790..20226)
                     /locus_tag="PKJ61_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016461622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HOC13605.1"
                     /translation="MTVTRPLRILFCSPRIPNNTGATMRLAAVTGAELHLARPLGFDM
                     DDAKLRRAGVDYRDQSATFVHDSMDEAFDALLPARVFAFTAHARTLYTDLAYQPGDVL
                     LFGPEPTGLDPEVLADPRITTGVRIPMRPGMRSLNLANAAAIAV"
     gene            complement(20231..21055)
                     /locus_tag="PKJ61_06985"
     CDS             complement(20231..21055)
                     /locus_tag="PKJ61_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015072036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOC13606.1"
                     /translation="MRDFLLDRLPEIAPVEEMLVAREFVDHAGRAWVGDDPYRPNSFV
                     WFHRYLLPEPVVPFPIEILYRDERILVVDKPHFLATTPRGTHVRESVLVRLRASLGLP
                     DLAPAHRLDRLTAGVLVLTTDRCHRAAYAGLFQSGAVRKTYEALAPADPDRTFPQRIG
                     NRIEKRRGSLQAEIVPGLPNAETLIEVVETRAQHARYRLTPTTGKTHQLRVHLNVLGL
                     PILGDPLYPTVRDVDPADFTDPLQLVARRLSFIDPIDRAPREFTSGFALEWPARAE"
     gene            complement(21154..21891)
                     /locus_tag="PKJ61_06990"
     CDS             complement(21154..21891)
                     /locus_tag="PKJ61_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012923252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOC13607.1"
                     /translation="MLDPEAADYPSDAASGVTLVELQEPLIECRACPRLVAWREAVAA
                     EKRAAFRDQEYWGRPVPSFGDPDARIVIVGLAPAAHGANRTGRMFTGDRSGDFLYAGL
                     WRAKLASQPTSEHSGDGLTLSGVRITAPVKCAPPANKPTPEERRRCAPWLARELALLG
                     ESVQVLIALGGFGWQALLETLAELGWLVPMPRPVFGHGAEVVLHAPDGRAVTLLGCFH
                     VSQQNTFTGRLTPDMLDAVLARAAELA"
     gene            complement(22080..22568)
                     /locus_tag="PKJ61_06995"
     CDS             complement(22080..22568)
                     /locus_tag="PKJ61_06995"
                     /inference="COORDINATES: protein motif:HMM:NF015331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HOC13608.1"
                     /translation="MEKTIEVDAPLRTVYDQWTQFEAFPRFMSGVKKVIQLSDDRLEW
                     VADLAGVRRQWIAKILEQVPDRKVAWTAVEGATNAGAAEFSEISPTRTQVHLCLEYAP
                     ELPRRAIVDPPDEGLIGTIPVGMPTEPGDRGLLSTDAAAGFGDPESEREPGVIETREP
                     DR"
BASE COUNT         3775 a         7595 c         7657 g         3496 t
ORIGIN      
        1 cgcgtccgcg ctgcccacca gccggcgcag caccgccagc gcctcggctt cgaagtctcc
       61 gatcccgtcc attcgagcag gctaaggggt ggcgctgaca gtttccccag cgggttggga
      121 aaggttccgt tccgcacccg aggtcggctc tgaccaggag cagaccgatg cggaaccgtc
      181 cccgaagcga ccctgaggaa ccgtccgcga agcgatccgg ctacaccgag accagcgcgc
      241 ccttcgtccg cggcgtgagc aggaacgcca gcaggtagag gatggccacc atcaccagca
      301 ggttgcggta gccggtgagc agggcgaagt actccaggca gccgcccaac atcgcgccga
      361 gcaggttcag cccgaacgcc gaccgcgaat cgtccgaggc tccgaagcgc ttcgagaacg
      421 ccacattcgc caggtagatc ggcacgaagg ccagcaggat cgccacgatc agccgcggcg
      481 cgaacggcag ggtcagcagc cagtccggct cgacgaagta ggtcaccgcc agcgatgccg
      541 cgatgccgcc gaagacgacc ggcagtgggg gcgtccggaa tcttcgggtc gtctcgaccg
      601 ctgccagcac gatcaccagc accccggcga acaccagcgc gttcaccagc caggtcgtac
      661 cgaacagcag cgcgaacgtc gcgatgttct tggtctccag cagcatgaac gccgcaccca
      721 tgaagaacag gtcggcatac gtgcgcatct ccttgaacgg gccgcccagc actcgcacgg
      781 cgatcaccga gatcagcagg atgccgccca gcgtccacag gtacagcggc gggatcggtc
      841 cgccctggta gtagaggaat ggacggtcgt cggtcgccgg cgccaccacc aaaccagccg
      901 gcgagtaggg cgtcccacac gactgcttgc tctcgtccag cgccgcgctg accacggcct
      961 ggccgttgcc tacgaggtct acgcagggca cgtgtccgaa cgcggcttcg gcggtcccgg
     1021 ccagccggtc gatcagccag tcctcgcggt agtagttgta catcgcaaac gcaccctcgg
     1081 gtgtgaggtg ctccttggcc gccgtcaatg cctgctcggt gaacaggaag ctctccaggc
     1141 ggatctggga cgcgccggtc accagggcca gcgagtcggg cagggcgaac aggatcaggt
     1201 cgtacttggt atcggtggct tcgaggtaag cgcgaccgtc gttcacgtgc cgggtcacca
     1261 gcgggttgtc gtagggatgg tcgggatgct tctccatgcc cagctgcatg atccgtggat
     1321 cgatgtccac ggcatccacg tgctgcgcgc ccttggccag ggtgatcgcg acgtccgatc
     1381 cggaaccggc gccgatgatc agcacgttgc gcagcgggtt gcccggcgtc cgctcgtagg
     1441 gcaggccgta ctgcggctcc tcctgcagcc gttgggacgc tgcacgcatg atctggtggg
     1501 gtacgccgtt caccgagata tcggtgagcg gaacaccggc cgaggtcagc tccttggtct
     1561 gcaccttgta gtacggcgac cacgacaccc cgggcgtaag cgtctcgagc agcagtgcga
     1621 ccaccatcgc tgcaccggcg accgcggcca ggtagcgctg ccagccgccg atcaacacca
     1681 cgaacgcgcc ggtcaccagc actccccaga ccaccgacgg cgcctgcagg aacgacagca
     1741 gcgtgaacga gccgatcccg atcagcgagc cgaccaggtc ccagcggtag gcggtgagcg
     1801 gacgcagccg cgcgaagcag cgtccgacca cttcggccgg gccggcgagc acggtggcga
     1861 ccaggatgaa gatcaacggc agcaccagcc acgcgggtgg cccagtggtg ctcagcgagg
     1921 tgaaatagat gatctggtcg ccggctcgat ctatggtcac cgggaagagg aaggtcgcga
     1981 tcaccagaac agcgaggatc accggggtgg ccggcagcac cgaccaggtg tagcgggcga
     2041 tcaggaaacc gaggccgatg ccgaggaacg agcccagcaa cacgaagttg gtgaaataac
     2101 tcagatgcac aacgttggac cccagccaac ggatcagcgc gagttccagg aacagcatca
     2161 acgcgctgcc gaggatcagc cgccgcacca ccgcgtagtc gggacgctcc ggctcgcggt
     2221 cggccggcag ccgccaggag cggcgcggcc gtgactggga agtcgtcttg gttgcgcggc
     2281 cttcgtcggc cattttgcac ctccgcccga gccggccgcg ggttccacgg gcaggctgtt
     2341 tggtcctcga atgacaccgg cacccggacg ccacgcccgc cgccgtgcgc tcgacactag
     2401 tggcgtcggc gtccgcccac taggttcggg acgtgatcgt gcgtccgatg cttgcccgcg
     2461 cctactgggc gctgtcccgc taccggctgc agtcggagcc gcccccggac acttcggcgg
     2521 tgctgatcgg cgcgccgcac acctcaaact gggacttcgt gatcatgctg gcgatcagct
     2581 ggcagcacgg tgtcgcgcca cgctggctgg gcaagcacac cctgttcaag gcaccgttcg
     2641 gacgcctgat gcgcgcgctc ggcggcatcc ccgtgaaccg ggccaacccg ggccgactgg
     2701 tggccgaggt ggtcgagctc gcccaggccg aggagcgggt gttcatcgtg gtcaccccgg
     2761 aaggcacccg gcatcgaggc aagctctgga agtccggctt ctaccgcatc gccagggacg
     2821 cgaaccttcc cctggtactc ggctacgtcg attcggccac ccggacggcg ggtctcgggc
     2881 cgaccatgca cctgagtggc gacgtggttg ccgacatgga ccagctgcgc gccttctacg
     2941 ccgacaagca cggtattcga ccggaggatt tcactccgcc ccggctccgc gaggaaggct
     3001 ccgccgagag ccccaagtag cgggcggaat ccacagacgg ttgcgcggta gctgggtaat
     3061 gggtggttgc aggaacccgt ccgcagctgg aggaaggcaa ccatggacca ataccaagtc
     3121 ggctacttcg ttggcagcct gtcgtcgaaa tcgatcaatc gggtgctcgc gaccgcactg
     3181 atcaagctgg cgccggccga gctctccttc accgagatcg agatcggcaa tctgccgctg
     3241 tacagccccg atttcgacgc tgactacccg cccgaaggac gcgcgctgaa ggatgcgatc
     3301 gccagcaccg acgcggtgct cttcgtcacc cccgaataca accgttcgat cccgggcgcg
     3361 ctgaagaacg ccatcgactg ggcctcgcgg ccgtggggtg agaactcgtt caaccacatc
     3421 ccggcggccg tgatcggcgc gtcgcccgga gccatcggca ctgcgctcgc ccagcagtcg
     3481 ctgcgcggcg tcctgagcta ctgcaacgcc cgccagatga ccgcaccgga ggcgtacctc
     3541 aagtacgacc ccgagatctt ctcagccgac ggcgaagtgc acgacgaatc ggtgaagcag
     3601 ttcctcaccg acttcatggg ctacttccac gtccacctgt cccgcgtgct gaccgtcctg
     3661 ccgcggtcga cgacctgacg gttagccgta ggcgggcacc accagcgccc accaggtcat
     3721 cggaccgacg atcgcgtcgg tggtgggcag gaacttgtcc tggaaagcgc ggacggccga
     3781 ctcggtcttc gggccgaaca ggccgtcagt agttaggtca ggtagtagcc actgcaccgc
     3841 ccggacggct tcgccgctgt cccccggcga caccgtgagc gccagttccg gccagtcgag
     3901 ctgaccgcag gtggtgctga tctcggtggc ccgaagtccg agttggaaga acttcatcgc
     3961 ctctccgctg agcgggccgt agctcccgtc cgcgacgatc gatgcgccat gcgctcgcag
     4021 caggtgttgg accgaacgga ctcgccagtt gctctcctgc gttgcgccga ccttcaccag
     4081 gggccacggg ccgtcctcgg tagtggcgaa cgaccaggtc tccggcccga ccgcgccgtc
     4141 gcgagtgagg ccccagttgt actggaagcc ctccacccgt tccttggtct cggggccgaa
     4201 caccccgtcc acgtccagcg gcccgtccca gggcggggtg gccaggcccc caacctgcag
     4261 ggccaggact gcgtcgccgg tgctgccgat gctgatcgtc ttaaccagct tgggccaggt
     4321 cttggggccc accacaccat ccgtggtcag gccggacgcc gcttgcagag ctttgaccgc
     4381 cttcacggtg gccgggccgt acgcgccgtc cacgttgagc tgatggccat gcgcccgcaa
     4441 caggtactgg atcgcagaca cccgctcgga ggtttcaccg gacgataccg tcgtccaagg
     4501 gaacaggttc atcgaacgct ccttccggcg ccgcgacgat gcgtccgcct gcgcccagta
     4561 tcgaccctca gttggctgcc ggacagtgct ggagttcccc attgagctac ggccagcgaa
     4621 gggggatgcc ggacggcgat ccacgaggat gagtacacga atggtccata gtgctgaggc
     4681 cccaggatct cgacggcccc cgaactgcgg ctcagaatgt tcgatatggc gcggtcggtg
     4741 tctgggggga ccgcgattcg cacacccgac cagctgctgc gggtcttcgt ctcgtccacg
     4801 ctcggcgaac tcgcgccgga gatcttcgac gcggcccagg ctcgtggccg cgaactcact
     4861 acaaaggagg cggtcgcgct ggcactgtcc accgaacagt cggccaaggt gaccgaactg
     4921 aagcccgggc gccccgcctt ccccgaggtc gctgtggcct cgtgagccac caggacgatg
     4981 gccgagcctg cgagctgcgc aacccaaccc aggatggctc agtctgacga agcctcaggg
     5041 cccttcgtca agctccggga tcggttggcc gacgatccgc aggaccgcct cgccgtactg
     5101 ctccaacttc ttcgcaccaa cgccgctgac acctgcgagc gcgtccagcg aatccggacg
     5161 cagctgcgcc atcccggcca gggtgctgtc cgggaacacc acgtaggccg gcaccgaagc
     5221 ctgccgagcg gtctcgccgc gccaggctcg cagcgcctcg aacagttcgc gatcttcgcc
     5281 gctcagctcg gacgccgcag ccgaccgtga cccgcccttc ggggccgccc tccggccgga
     5341 acgttgcgcc cgcgcaacgg cgtcttggcg tagcggcacc ggctgctcgc cccgcagtac
     5401 cggccatgag gcttcggtga gcccaagagt gccgtgcccg tcggcggaga ccgcgagcaa
     5461 cccagccgcc agcagctgcc gcgtcacggc ccgccactgc tggtcgccga gttcggtgcc
     5521 gatcccgaag gtgctcagtc gctcatggcc gaaccgctgc acccgctcgg tcgagcggcc
     5581 aagcaggatg tcgatcaact gaccggcgcc gaagcgctgc ccgtgttcac gttgcaggcg
     5641 gacgatcgtc gagagcagct tctgcgcagc cactgtcccg tcccaggcct gtggtggtgc
     5701 caggcaggtg tcgcagttcc cgcacggttc gctggcctca ccgaagtagg ccagcagcaa
     5761 ctgccgccga cagcgcaggg tctcacacag tcccagcatc gaatccaggt gttgtccgag
     5821 cagtcgcttg tgctgcgcgg agccgtccga gccctcgatg agttggcgct gctgcaccac
     5881 gtccgccagg ccgtaggcca tccaggcggt ggccggcagt ccgtcacggc ccgcgcgtcc
     5941 ggtctcctgg tagtagccct cgatgctctt cggcaggtcc aggtgcgcga caaagcggac
     6001 gtccggcttg tcgatgccca tgccgaacgc gatcgtcgca accatgacca cgccgtcctc
     6061 gcgcaggaac cgggcctggt tggccgagcg gacgttcgtc ggcagcccgg cgtggtacgg
     6121 cagcgcggtg atgccctcct tgacgagcgc ggtggcggtc ttctcgaccg atgcccggga
     6181 caggcagtag acgatgccgg cgtcgccgtc gtgctcgtcg cgaatcaggc tgaccaactg
     6241 gcgcagcggc tgctgcttgg gctcgatccg gtactggata ttgggccggt cgaagctggc
     6301 cacgaaggtg cgtgcgtcct gcagcgcgag ccgctcggtg atctcctgtc gggtcgccgg
     6361 cgtagcggtt gcggtcaggg cgatccgggg gacgtccggc cagcgctcgt gcagcaccga
     6421 gagccgcagg tagtccggac ggaagtcgtg cccccattgc gacacgcagt gcgcctcgtc
     6481 gatggcgaac accgagatct gcagccggtc caggaactgc tgcgcccatt cggtcccgag
     6541 gcgttcggga gccagataga gcaggtccag ttcgccggcg atcgcggcgc gctcggtggc
     6601 acgccgctct tcggccgaga gtgtggagtt ccagaagccg gcccggacgc ccagcgcgtc
     6661 cagcgcggtc acctggtcgt gcatcagggc gatgagaggc gaaatgacca cgcccatgcc
     6721 cggacgaact atggctggaa tctggtagca cagcgacttg ccgccgccgg tgggcatcag
     6781 gacaaccgcg tcgccaccac ggacgacctg atcgacgatc gcgccctggt caccccggaa
     6841 cgcggcatag ccgaaagtcg accgcagcac ctcaagcggg tcagcggacg caacaggcac
     6901 cgcgtcagga tagccagccg cgccgaaaac aaccgcgcac gagccgcggt cccacgcccc
     6961 acggacggat tcgcgcgcca cattcctatc cgagccgttc tcgggaaact cgcggctgct
     7021 accgcgcaaa tccgctcgcg ttagcacagg cgaggcgtcc agggctgctg ccgaaccggc
     7081 cacgggcgga tgccggctgc ccggccgagc cgggttaggt tggcagcatg tacttgggcg
     7141 tcgacttcgg aaccacccgc accatcgtcg tcagggcgga ccgcggcaac tatccggttg
     7201 tcggcttcgt cgaccccgcc ggtgacaccg ccgagttctt cccttcggtg atcgcactgg
     7261 cggataccca acccatgtac ggattcgccg cccaggtcgc cgcgcgcaac ggcgcgccgc
     7321 accttcgctc gatcaagcgg ctgctcggcc aaccgggcgt aaccgcgacc accggggtcc
     7381 ggcttggcga gcgcacctac ccgctcctgg agctgctcac cggtttcctc actgaactcc
     7441 ggcggcagtt ggtcgaacac tccgacctga ctggcgaact gaccgcaaga accgcactcc
     7501 ccacgctcgt ctcagtaccg gcccatgccc acggcgcgca gcgcttcctc acccttgagg
     7561 cgttccgcgg cgccggcttc gatgtgaccg gcctggtcaa cgagccgtcc gcagccggct
     7621 tcgagtacac ccaccgcctg ggtggaaccc tcacctcccg ccgcgaccgg gtactcgtct
     7681 acgacctggg cggcggcacc tttgatgcgt ccctgctcgc agtggccggc agcagccacg
     7741 acgtgatcgg gtcggtcggg gtgaaccggc tgggtggcga tgacttcgat accgcactcg
     7801 ccgagctcgc actggaggcg tccggccggc cggagctgga cgaggccgac tacggcgcgc
     7861 tgctggccga gtgccaggaa gcgaaggaac ggctcagccc gcaatcccgc cggctcaccg
     7921 tcgaagtggc cggccagcca gtgctggttt cggtggcgga cttctacgcc gcggtcaccc
     7981 cgctggtgaa cagctcgatc gaagccatgg caccgctggt ggccgccctc gacgcggacg
     8041 ccgccctggc tcaggtcgcc ggcctctacc tggtgggcgg tggcagcggg ctgccgctgg
     8101 tgccgcgact gctgcgcgag cgattcggcc gtcgggtgca tcgttcgccg tatccagcgg
     8161 cgtccaccgc gatcgggctc gcgattgctg ccgacccgga ttcgggcttc tccctggccg
     8221 accggctgtg gcgcgggttc ggggtcttcc gcgaactgga cgcgggccgg gccgtcggct
     8281 tcgaccagtt gctcgaccgg cacaccgaac tgccccgttc cggcgagctg accatccacc
     8341 gccgctaccg ggccgcgcac aacctcggct ggtaccgctt cgtcgagtac gccgacgtgg
     8401 tggccggcga gccgcgtggc gacctggcgc cgttcgccga ggttcgcttc cccttcgacc
     8461 ccgcactgca cggacgcgac ctcaccagca tcacgatcga gcgcaggccg gacggcccgc
     8521 tcgtcgagga ggcctactca ctggacgcga acggcatcgt cacggtccgc atcaccgaca
     8581 ccgctaccgg ctacacccag acccacgccc tgggctgagc cggcagctcc gtcccctatt
     8641 cagcctgggt gatctgcacc tcgaccgact gtccggaact cggtccaccg gcgaagaccc
     8701 cgctgagcgg cggcacatcg gcgtagtcac ggccacgggc caccagaacg tgcgctgccc
     8761 cgaccctgcg gtcgttggtc ggatcgacag cggtccaccc gccgtcccaa cactccaccc
     8821 aggcgtgtga ctcacctacc acggttcgac ccggggacgg ttcctcggtg tgcaggtagc
     8881 cgctgatgta gcgcgcggga atcccaaggg cacgaaggat cgcgagggtg gcgtgggtga
     8941 agtcctggca caccccgcgg ccggccgtcc acgcttcgct cgcggtggtg gtgaccgagg
     9001 tgatgccagg ttcgtaggct attcgttccc gaaccgcacg gacgcaaccc agcaccgcgg
     9061 cacgcgggtt gggagcggtg gccaagtccg caacaagatc agcagccacg ccaaccggaa
     9121 gctcggcatc ggcgaggtca gtgaaaccga ggaactcaca gttggcatcg acgaagcctg
     9181 gatcccgcaa gtcgtcccaa cccaccggct caccgagttc acgtgcgcca ggtaccagga
     9241 cggtgctagc ggcccggatg tcaagctccc ggtgcgggga atgcagttcg atcgtggtca
     9301 cctgcgcacc ccagtagtcc acatggcggc tcagcgaggc tgacggcttc acccgtacct
     9361 tgtgcgctag cagcacctgg ccgttctcgc tgagtggggt catccgggct tcggtgaagg
     9421 acgcgctgac atcatcggag tagcccagcc gggtttgatg caggatttcg agtcgccggg
     9481 ccgacccgac cggtgcaaag gcgtccatca gtggctccag acgatcgtgc cgtgctggcg
     9541 gaagtaggcg gcgttcacca ggtcggcggc ccgggcacct gcggccgcgg cggtctcgat
     9601 gagtgcgtcc accacccctg gctgcccagc actggcgtac tcgagattcg agcgcagcat
     9661 gccgacctcg ctgagcagtt ggcccccatc gctcacccca gaggacgcga gtgctcgcac
     9721 cgcctgctca gcatcatccg cgcaggcccg catcgagcgc gggaacgttc gatcgagcac
     9781 caggaaggca cgcaccttct cgcccaccga cggctcaccg gtgcgcagga atgccgacaa
     9841 cccgccacag gcccgcagta cggctgccgg cccgatggtc ggccaaagca ggtcatggcg
     9901 catggcgagc atgcgtccca tcatgtcgat gcgctcgagt tcgcgtccga gcacgaggaa
     9961 gtcgtgggcc tcgtcgcgtg gcatggtcca ttcgaccacc ccgttgacca cgagcaggcg
    10021 ctccaggagt cgatgcaggg cgacgcccgg gctggtgccg cggccgaagc cccgcgccaa
    10081 cagtatctgg acggcgttga tcgattcgta ggtgtcgctg gacagcgcct cgcggactga
    10141 tcgagcgttg tcgcgagccg cactcaccgc acccgagatg gtggccggtt cgctcggcga
    10201 gccgatcacc agccgggcga ggtgctctcc cgtggttgga cgcgccgagg aaatcgccag
    10261 cgcatcgacg aggacgcccg cagccagaga gaactccacc gactggtctt ccaccaccag
    10321 ttggtgatgc tcggccagca acctggtggt ggcttcggca cgctcgaggt agcgacccat
    10381 ccagtagaac gattcggcta tccggctcag cattcggcca acctctcgcc ggtggcatcc
    10441 gtggctgcgc cagcctccgg ctcgacgtct ggttgcgctg cctgctgctg ttgccgctcc
    10501 tgttgcgcca gccggtcgtt gtgactgggg accgcgttgg ggcgcgcagg cagccaggct
    10561 gaaaccgggg cgaccctgat cactaccggc accgcctcga cttggggggc ggcggagtct
    10621 tcgagcaccc aggtgtcctt cgaaccaccg ccctggctgg agttgaccac caggctgccg
    10681 gccaccagtg ccacccgggt cagcccaccc ggcagcacgc gcacccggtc gccgtcgttg
    10741 gtggcgaacg gacgcaggtc gacgtgccga gatgccatct cgccatcgtc gccgacaaca
    10801 gtgggacagg tcgacagccg caccaccggt tgggcgatcc agccacgtgg attggcctcg
    10861 agcgccgcga tcgtggcccg gatctggtcc tcggaagcgg acgggcccat cagcagcccg
    10921 tagccaccgg acgcgtctgc cggtttgacc accatccgat cgatgttcgc aatcacatgt
    10981 tcgcgttggt cgacatcggt gaggtcgtag gtcgggacat tctccaggat cggctcttcg
    11041 cccaggtagt agcggatgaa ggccggcacg taggggtaga tcgccttgtc gtcagcgact
    11101 ccgttgccga cgaagttgcc gataccgacc gtgttggccc gcaccgcatt gagcagcccc
    11161 ggcacgccca ggactgaatc tggacgcatc accagcggat cgagatagtc gtcgtcgatg
    11221 cggcggtaga tcatgtcgac ctgccgaggg cctgaggtgg tgcgcatcca caccttcgag
    11281 tcgcggcagt acaggtcgcg tccctcgacg agctcgatgc ccatgtgacg ggcgagaaag
    11341 gcatgttcgt aatgagcgga gttgtgcacg cccggcgtca ggaccaccac ggtgggctga
    11401 ctgcctgcgg tcggcgcggc tgcgcgcagc gcatccagca ggtgctcggg atactccccg
    11461 accgtccgca cccggtagtc ggcgaagatc tcgggcagca catgcgccag cgtgcggcgg
    11521 ttctcgagca cgtaggagac cccggacgga ttgcgcaggt tgtcctccag gacgcggaac
    11581 acccctgcct cgtcccggat caggtcgatg ccggacacgt gaatgcgcac cccgttcgcc
    11641 ggacgcaaac cccatgcctg gcgggcgaag tgggtggacg tggtgaccag actgcgtgga
    11701 atcacccggt cctggaagat catgcccggt ccgtagacgt cggccaggaa cgcctccagc
    11761 gcgtgcagcc gctgcgccac cccggcttcg ataacggccc agtctgatgc cgtgatcagc
    11821 ctcggtacta ggtccatcgg cagtggacgc tcccgctcgc cgagggtgaa ggtgatgccg
    11881 tgccggtcca gatatgcgtc ccgctgccgc gcccgctcgg cgcgcccgtc cgggccgagc
    11941 cgctcgaggg ccgcgaggag tggctcgtcg gcagcacggg gtccgccagc gtccagcatc
    12001 tcgtcgaacg ccgcgggtgt gctggtcacg gccgtcatgg gtggaaacgt agggcggtga
    12061 gtttacctcg gtgttgcggt cacgcgtccg tggcgttacg cggtggccga ggttgcgtcc
    12121 ggtgcatccg tttcgaggtc gtaggcaggc tgttcatggc tgaaggcacg gtgaagcacc
    12181 atgtctcgac acggttgcgc aagctggccc ggcgcgaccg caccgctctg gccttgttcc
    12241 tctcccgcca cctgtaaccc gtcggacgcc cggctcgtca acccggccgg ggtaggctga
    12301 agcgaaaccg ccctcgacgt ctgccgaaag cgctcgcctt gtctgcacca tcccagccag
    12361 ccctgaagct gctgatcgct gcggcgacgg ccgttgtcgt gaccgtcggc ctgatgccgt
    12421 cgaacgcgga cgcgcgcccc gtggctccgg ggcctgccgc ggctccagcg gcgctgccgc
    12481 tggcggaacc cctgcccggc ccggtgatca ccgtcgacct ggtggacgcc gtgatcggac
    12541 gctacggcac caggaccagc gcgaccttca aggtggaagc gaccggagag aagctgcgct
    12601 accagtggca gcgactggcc ccgtacgcga agaagtggaa gaacgtctcc ggggccaaga
    12661 aggcgaagta caccgccaag gccaccacct gggccagcgg caccagtttt cgggtcgtgg
    12721 tgaccggcac cgggggcaag gtgacgtcgg cgccggccaa gctgaccgtg ctgtacccga
    12781 ccaagtcccc ggccaaggac gcggaagcgg cgttcggttt gaccggactg cggcagggtg
    12841 tcgacctttc ggcctaccag tggacgcccg cggcaaagat caggatgaag aaggtggacg
    12901 cgtgggccgg caaggacggc ttcacgatcc tgcggaacgg cagcggctcg cgtccgatca
    12961 agcagcagta caccgacgcg tgcaccaaca aggcccgcaa gaccgggacc gagccggtca
    13021 ccgaggattg cgcctacgcc aagctcgcca agcaggcgaa atcggccggc ctgtcgctcg
    13081 gccactactg gttcaacggc tggatcgcgt ccacggattc gacgacccag cagctgttcg
    13141 cgggcggcta ctcggccacc gcgtccgccg aacagttcgt caagtggctc aagcgcgacg
    13201 gtcgctacac caagtcgagc accgatccgc tggtactgga catcgaggcc ggtaacgctt
    13261 ggaccaaggc caaggacggg gagaagatca agcagaagct gcgggcgtgg aacccgagcg
    13321 aggccgccga gttcctgacc gcggtgaagc agctgctgac cgccgacggc taccaggcga
    13381 acctgtacgt ctacatgagc gcgaaccgca ctttgcagct gggtccgacg ggatacctgt
    13441 ggcaggacgt ggccggtatt gcaaagctct gggtcgcgtc ctggggcacc aacaatggcc
    13501 gcattccgga cgcacagcct gaggtcggtc cctgggctga ggcgggtggt tggtcgatct
    13561 ggcagtacac cagcaacgcc aggttcagcg gctccggcgt gggtgcgctg gacggggaca
    13621 tcgcgaaggc cgacgcttgg acgccactcg ccggctgagc gtccgttccg ctttccgtca
    13681 tcccggcgaa ggccgggatc ccggcaggac tccggcgtgg tgggccagag atcggtctat
    13741 tcggcgccgg cggctcaggg ctgcagcagc gagagcgaca tggccatggc gaagccgagc
    13801 acggtggcca gcccggcctt gccgctggcc ttcgactgcg cttcggggat catcgagtcg
    13861 gtgagcatga ccagcagggc ccctgcggcg aagccgttga caccggcacc caccgccgcg
    13921 ggcagcactt cggccaaccc gaagccggcc agcgtggcga gggtggaaca gaccgccacc
    13981 accgtccaga gcccaaagat ggagcgccgc gagcgaccgg cgtcccgcat ctctgcggca
    14041 gagcccagcc cttcggggag gttcgacacg aagatgctcg cgaacagtgc caggctgatg
    14101 ccgccgccac tggccaggcc gagccccagg acgagctgtt cgggcacgcc gtccagcagt
    14161 gcgcccagag caagcggcag tccgctggac gctcccgagt tcaggcgctc caccgcccgg
    14221 tcggcaaggt agaagacgag cgcgcccaag gcgaggccaa tgccgatcgg cgccggcccg
    14281 gacgttcgca gcccgtcctc gaccagttcg taggcaacgc ttgagatcag cgcgccggcg
    14341 ccgaaggcga gcaccatgcc gaccgcccca cgatgccagc tgcgaaccag cccgagaagt
    14401 gcgccgatca ccaccgagga cgccgccagg gcgccccatc cgagtgctga ccacatggcc
    14461 acatcatggc agccggccac gtccgaactc ataggctggc caaggtgact tccgactaca
    14521 ggttcacgac cgaagaagac gcggtcgatc accccctggt acaccgctgg ctgagcgagc
    14581 agtcctactg ggccaacggc cgctcccgcg acacgctgga tgctgcgatc gacggctccc
    14641 gcaactacag cgtgctcgag gtcggcttgt atcggcagtt cggcttcacc gcactcgccg
    14701 agccgggacg ctggatggtc cgcggtcgaa gctgatcgcc aacaatgtgc aagatcttgc
    14761 accgaactcg gtgcaagatc ttgcacgtcg cttcagcgag cgtcagtcga aggcggcgac
    14821 ctcgatcttc ttcatgttca tcagcttgcg gtaggcgtcc ggcttggtca tcagctcgcc
    14881 gaggttggcc gggatgaact gccaggcgac gccgaacttg tcctggcacc agccgcactg
    14941 ctcggcctcc gggaccgcgc tgagctggtt ccagtagcgg tcgagttcgg cctggtcggc
    15001 gcactcgacc atcagcgaga cgccacagtt gaaggtgaag tcctgttcga cgcccgaatc
    15061 catgaccgcg aaccactggt caagaatctg gaagtccgcg tacatcaggt tgccgggctg
    15121 cgcggggccg gtcggctgtg ggtagggaga ggaggccagg cgcttcatgc gcgaggccaa
    15181 gagagatcgc gtggtcgcca cccgactggc ttcgatcggg tcgctgagct cggctggatc
    15241 agcggggacg agcgggatgt gctggtcaag ctgtaccagc cgggcggcag caaagccgcg
    15301 aaatcggcga aggtcgcaca aacggatgac cagcgggtgc ggaccgctgc ccgcgactac
    15361 ttgaacctgc gcaagagcca tgccgggctg ctcgcccgcg gggatgcgag cccggtcgca
    15421 ctggcgccgg cgtccggcgc ggtggggtcg tccgaggccg aagccgggga cgacggcact
    15481 acggtggtgt acgcgaagag caaccgcagc taccagggca tccttggcgg cgccggggcc
    15541 atcatcggcg gtgcgctcgg cggtgccccg ggtgcagcta tcggcggggc aatcgggggc
    15601 gtaatcggca tgatcgtcga cgactgaaag gactgaactg ccggggggct cagggccggt
    15661 cgcgcttggc ctcggcgagc cattcggtgt cgaaggccag gttcagcttc tccaaccggg
    15721 cgatgtcggc ggcgacgtgc tcgcagtgct tgcgcacttc cggctggccg gccgccgtct
    15781 cgttcagttc gtgccagatc cgcgacaact ccgcgatcac gagtttgttg gactcgatta
    15841 cgcggacccg cgcgtccgca gcctgcgcct tcagttcacc aacacgatca ctcggttcgt
    15901 acatgtgccc atgctaggac gcgcagcgct gggcagccgg tggttgaggt gacagactcg
    15961 cgggcacggc gaccaagaac gagggcctga ccgcggagga gaaggccgcg ctgaagcaga
    16021 tgacagcgga gcggcgacgc tcggcggcgg gcaagaacac cgaagcggac gtgctcgcgg
    16081 cgatcgaggc cattgacacg aacgttcgtc cgatggccga gggactgcat gccctggtga
    16141 aacgcgagct gcccgagctg accagcaaga cctggtacgg cttccccgcc tactgcaagg
    16201 gcggtcaggt ggtcttcttc ttccagtacg ccggaaagtt caagtcgcgt tacggccaca
    16261 tcgggttcca ggacaccgcg gcgctcgacg acggcgtgat gtggcccacc gccttcgcgg
    16321 tcaccgagtg gaacgctgac gtcgaggcgc gcgtcgccga actgatcaag cgtgcggtga
    16381 gctagccctc cgacgacggc tgggtgggtt cagacctcgg cgatcaggtc ccgcattgtg
    16441 cggatctcgg cgcgcaaacg ggcagcgatg tcgccggctt cggcggcgtc cggacgcgac
    16501 atggccgcgg ttacggctgc gaggacgatc ccgaacacga tgcgcgcggt gtgggcgtcc
    16561 cccgtccgct gctcgatcag cttgaagaac ggcttgagcc gggcgatggt ctgcgtccag
    16621 aagtccatta ggagttccgg ctggccgtgc atcagggctt tgatccgagg gaagtcggtg
    16681 ggcagggtga cgttgcgtcc gatcaggtca caggtgtcgg cgaccagact cccgtccgca
    16741 ttcaggaacc attccacctg gtcggccggg atgtcggtga ccagcaggtc gaacgcggcc
    16801 gctaccttgc tcgggtagta gttgaagaaa gttcgcggcg agaccccgac ctcggcgcag
    16861 atctcctcga cagtgacgct ttgcgggccg cgctcggcaa ccaaacggag ggccgtctcg
    16921 tgaatcgccg tccgcgtcgc cgacttcttg cgctcgcgca gaccactgac cggttcgctg
    16981 gtcacctgcg tcatgcgagt ccctccagag cgtccgactt ctcctgcagc gcggagcggg
    17041 tgcgcagcgg cggcaccttg aagaacaggg tgaccacgaa ggcgagcacc atgaccccca
    17101 gccccaccca gtagatcgta atcaccgaat cgctgaagcc caccatgaag ggttgggtga
    17161 gccgcgggtc ggccccggtg aggaacgagg tgtcgctggt gctgctgtcg gtgttcacgt
    17221 cgctgcctgc ggcgtcaccg ttcttgatcg tctgcaccag tgtcggcgtg agctcgtcga
    17281 ccatcgccca gcgctgcacc ggatcggacc agtcgatggc gagcacgcca tccttgacgc
    17341 tcaggttccg ctcgaaagcg acctgctgca aggccttcgc gtaggcgtcg ggcagtgcct
    17401 tcttgacggc tttgtcgatc agctgatcaa ccttcgacag ctgcttcgca ccactggcca
    17461 gcttcgcact gccggacgcg gccgcgctcg cccccgtcgg gatcgcgctg cccttcgctg
    17521 cggcggtcgt cgcgccgtcc gcgagcttgt gggtgccgga cgcgagctgc tccagtccgt
    17581 ccgccagcgc cgaggtgccc ttggctgcgg acgccaaccc ggcagccaga ccggactgtc
    17641 ccgcggcgcc gttgagcatg ccgctggtgg tgtagacgtt gccctgcagc gagcccatgg
    17701 cggcgtcgac ctccgcctga tcggcaacga gctggtcgca ggcggcggtc tcgccgctca
    17761 cgcagctgtc ctggtcggcg gtcatggtcc tgatcgcggc cgaaagggtg acgtagtcgc
    17821 ccgcggcggc cttggccttc gcggcggcgg tctgctcggc gtcgctgagt ttggccagtc
    17881 cgctggcgag ctgattctcg gccttggcga tcttcgaggt gccggtggcc aactgctgtg
    17941 ctccggagcc gatcttgccg acgccgtcga cgaaggcgtc cgtcccgagc gcgagcttgt
    18001 cgagaccggt gccgagcgcg ttgatgccgt cggcgatctt gccggtgccc ttggcggcct
    18061 tcttcgacac cgcctcggtg accttggcgc ggacagcggt ctcaacaccg tccttgacct
    18121 gctcggtggc gtcggcgagc ttctcgtcca cctggtcctt gatcttgtcg gtgatcggcg
    18181 tccacagctt gtccatgatc gcggcgttgg ccggatctcc tgcgaccttc gggttcagcg
    18241 ccgcgtccag cgcgtgccag gtggtgtgct cgtcggtcag cgcgccggag atgttggtgg
    18301 gcaggatgct gaacagcagc gacagcatga tcgccgtacc gagggtgccg ccgatctggc
    18361 ggaagaaggt ggccgaactg gtggccacgc cgatgtcctg ggcgtccacc gcggcctgga
    18421 cggccatggt cagggtctgc atcagctggc cgaggccgag gccgatcacg aacatggcga
    18481 tcgccagata ccagtacggc ttgtcgaggg tcatgagcgt gagcagaaag aagccgaccg
    18541 cggtggtcgc agtcccggtc ttcggaaagg ccccgtacct gccggtgcgg gccacgatct
    18601 ggccggtgcc gactgaggcg agcatgatgc cggccatcat cggcagggtg gcgaagccgg
    18661 attcggtcgg gctgaatccg agcacgatct gcaggtagag cggcagtgtg agcatggcgc
    18721 cgaacatgcc gaaaccgacc aggatgctga gcacggtggc ggtcgagaac gacttgttgt
    18781 cgaaaagccg cagcgggagg atcgcgtccg gccccatcgc ccgttcgacc aggatgaacg
    18841 cggtcaggct ggccacgccg attgcgtagc agatcatcgc gggcacggac gtccagcccc
    18901 actcgcgtcc ctgctcggcg accagcagca gcggcgcgag ggtggcgacg accaggccgg
    18961 cgccccacca gtcgatgtgc cggcgaccac gcgggtggtg cggcaggtgc aggaaggcga
    19021 ccaccatgaa cagtgcgatc acgccgaccg gcacgttgat caggaacacc cagcgccagc
    19081 ccgagatgaa caggatctcg tcggcaccgg cgaacaggcc gccgacaagc gggccgatca
    19141 cggacgcgat gccgaaggtg gcaaggaaga agccctggta cttggcgcgt tcacgtggcg
    19201 ccagcatgtc gccgatgatt gccagcggca gggacatcag accgccggca ccgagaccct
    19261 ggaacgcgcg gaagccggcg agcatgatca tcgaggtcga gaagctgctc aggaacgaac
    19321 cggccacgaa gaggctgatg ctgatgatga acagcggacg gcgtccgaag atgtcggaga
    19381 gcttgccgta aagcggggtg gtgacggtgg cggcgatcag gtaggcggtg gtgacccacg
    19441 cctgctgatc gaggccgtgg agttcgtcgc cgatggtccg gatagcggtg ccgacgatgg
    19501 tctggtcaag cgcgctcagg aacatgccag ccatcagcgc gtacatgacg agcatgatct
    19561 ggccgtggtt catgaccggt tggttcgggg tctgctctat cggggcggtg cgctccacgg
    19621 tggcaactgc cacttctcct cctacaaacg aaagcgccgc attcttgcag cggcgcaggt
    19681 ctacagtagn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna cggcgatggc
    19801 ggctgcgttg gcgagattga gcgaacgcat gccgggtcgc atcgggatgc ggacgcccgt
    19861 cgtgattcgc gggtcggcca gcacttcggg gtcgaggccg gtgggttcgg gtccgaacag
    19921 cagcacgtcg cccggctggt aggcgaggtc ggtgtagagc gtccgggcgt gcgcggtgaa
    19981 cgcgaagacc cgcgccggaa gcagcgcatc gaacgcctcg tccatggagt cgtggacgaa
    20041 ggtcgcggac tggtcgcggt aatcgacacc ggcccggcgg agcttcgcat cgtccatgtc
    20101 gaagccaagc ggacgggcca gatgcagctc agcgccggtg accgcggcca gccgcatggt
    20161 tgcgccggtg ttgttcggga tccgcggcga gcagaacagg atgcgcagcg gacgggtgac
    20221 ggtcatgggg tcactctgcc cgcgcgggcc actcgagtgc gaacccgctg gtgaactcgc
    20281 ggggagcccg gtcgatcggg tcgatgaagc tgagccggcg ggcgacgagt tggagcgggt
    20341 cggtgaagtc ggccgggtca acgtccctga cggtcggata gagcgggtcg ccgaggatcg
    20401 gcaggccaag gacgttcagg tgcacccgga gttgatgggt cttgccggtg gtcggggtca
    20461 gccggtaacg ggcgtgttgg gccctggtct caacgacctc gatcagggtc tccgcgttcg
    20521 gcaagccggg cacgatttca gcctgcaggc tcccccggcg cttctcgatg cgattgccga
    20581 tccgctgcgg gaaggttcgg tcggggtcgg ctggcgctag cgcctcgtag gtcttgcgca
    20641 cagcaccgga ttggaagagg ccggcgtagg cggcgcgatg gcagcggtca gtggtgagga
    20701 cgagcacccc tgcggtgagc cggtcgagcc gatgggcggg cgccaggtcg ggcaggccga
    20761 ggctggcgcg caacctgacc agcacggact cccggacgtg cgtgccacgc ggcgtggtgg
    20821 ccaggaagtg cggcttgtcg accaccagaa tgcgctcgtc gcggtacagg atttctatcg
    20881 ggaacgggac gacgggctcc ggaagcaggt accggtggaa ccagacgaag gaattgggcc
    20941 ggtaaggatc atcgccgacc caagcacgtc cggcgtggtc gacgaactcg cgcgccacga
    21001 gcatctcctc gaccggcgcg atctcaggca gccggtcgag caggaagtcg cgcatggtgg
    21061 tccatcgcgg gacggtgtcc gggtcgttgt ccggtgtgcg cagccaggcc gcgtccagcc
    21121 cgtcccgctg gggcagcggc gatcggggcg gcactacgcc aactccgccg cgcgcgccag
    21181 aacggcgtcg agcatgtcgg gagtgagccg tccagtgaag gtgttctgct gcgaaacgtg
    21241 gaagcagccg agcagggtga ctgcgcgtcc gtccggtgcg tgcaggacga cttcggcacc
    21301 gtgcccgaac accggtcgcg gcatgggcac cagccagccc aactcggcga gcgtttcgag
    21361 cagtgcctgc cagccgaagc cgcccaatgc gatcaggact tgaacggact cacccagcag
    21421 cgcgagttcg cgggccagcc acggcgcgca gcggcgccgc tcctccggcg tgggcttgtt
    21481 ggccggcggg gcgcacttga ccggcgcggt gattcggact ccgctgagcg tcagcccatc
    21541 gccgctgtgc tcggacgttg gctggctggc cagcttggct cgccagagcc cggcgtagag
    21601 gaagtcaccg gaccggtcac cggtgaacat gcgtccggtg cggttggcgc cgtgggcggc
    21661 cggagcgagg ccgacgatca cgattcgggc gtccggatca ccgaaactgg ggacaggacg
    21721 cccccagtac tcctggtcgc ggaaggccgc gcgcttctcc gcggcgacag cttcccgcca
    21781 ggcgaccagc cgcgggcagg cgcgacactc gatcaggggc tcctgcagtt cgacgagggt
    21841 cactccggac gcggcgtcgg aggggtagtc agcggcctcg ggatcgagca tggccaccat
    21901 gctgcctcaa gctggccgct gatgatgacc caaggggaat cggtgcccac caagccaaat
    21961 ccgcgcggaa cgaatcgcgt ccgatcagcg gtggctgtcg gcgtgtctgc gtaagtcgct
    22021 cgggaggaat cagtttggcc cgccgtcgga gtcgcgccga ccaaccgaaa cagagcctct
    22081 caccgatcgg gctcgcgagt ctcgatcacc ccgggctcgc gctcagactc gggatcgccg
    22141 aagccggccg cagcgtctgt cgacaggagg ccgcggtctc cgggctcggt cggcatcccc
    22201 accgggatgg tcccgatcag gccctcgtcc ggcgggtcga ctattgcgcg cctgggcagc
    22261 tccggcgcgt actccaagca caggtgcacc tgagtccgcg tggggctgat ctcgctgaac
    22321 tcggcggcgc ccgcattggt ggcaccctcg accgcagtcc aggccacctt acggtcgggc
    22381 acctgctcaa ggatcttggc aatccactgg cgtcgcacgc cagcaaggtc cgctacccac
    22441 tccagccggt cgtcgctcaa ctggatcacc ttcttgacgc cggacatgaa gcgcggaaac
    22501 gcctcgaact gcgtccactg gtcgtagacg gtgcgcaagg gcgcgtccac ctcgatcgtc
    22561 ttctccacct aggttgtcac ttgagcctcc agtcaacgac ggcgggttca cccgctcacc
    22621 atc
//
LOCUS       DAOPRQ010000042        22404 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_1925_length_22404_cov_11.288917, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000042 DAOPRQ010000000
VERSION     DAOPRQ010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 22404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22404
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1925_length_22404_cov_11.288917"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1116
                     /locus_tag="PKJ61_07000"
     CDS             <1..1116
                     /locus_tag="PKJ61_07000"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOC13609.1"
                     /translation="NSRPTGNQTGRHTDGLFSKLLEPNPFVDPGYLLPALRWDGQGAG
                     IKLVVVMDGEDWIAALPLSVMPPAWKLPLRLATTAGPFLSRRAPLCAPLVDRARAEPA
                     IERLMEHLASPASGLPGLVELTLLPVGRLTELIRAECDRKRVRCLERYRFERACLVRS
                     AGKSPRGSLSVRRRKLLARKERGLERSLGGPLELVDLGCDQAAVDLFLDLEASGWKGA
                     RGSALRRKPGAAAWFNEVSAGFAERGMLRILALQAGGETVYSAVQLLVGNTVFGMMDA
                     YEERWSAFTPGVIGRVREQEEFMDRGSAELLDPCIHPRYVQPSALYPDRRLIVGLVLA
                     PRGLINSQFLRLRPGMILVRDKYLKVAAAVSGWMRRG"
     gene            1253..1912
                     /locus_tag="PKJ61_07005"
     CDS             1253..1912
                     /locus_tag="PKJ61_07005"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOC13610.1"
                     /translation="MSTTESVFGLSYDDGPDPEQTSAILDVLARHCAHATFFVLAGPA
                     AKHPEILRRMVAEGHEVALHGLDHASLLTRSTSAAVAAVLEARAVVESIVGTSVRHYR
                     PPYGAHTLRQARAIHRAGLELVVWSGDAKDWVQADEDVIIKRAWATVFPGAFLLLHDT
                     RADPETALDPSELPTFDRAAVLEDLLLRATAAGYSALTVGDLMDRFPAVKSWARERSR
                     M"
     gene            1909..2886
                     /locus_tag="PKJ61_07010"
     CDS             1909..2886
                     /locus_tag="PKJ61_07010"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOC13611.1"
                     /translation="MTDTESIPELSVVIAAFNAGGTLGEQLRELAAQTPQWPWEVIVA
                     DNGSTDRTTEVVAELQADFPWLRLVDASQRRGPAAARNVGVTYARAARIAFCDADDLI
                     ADGWVETMHAALVEHEFVAGSFDTRRLAGRNRFVVSWSPQREALSVKPYLPEFVTGGS
                     GNLGIRRSVFNEAGGFDESAMVAEDDDLCLRVQLAGHQLVFESSLVLHVRRRAGLRAV
                     LRQARAYGAGARRLEHRYARIVARLHPAVPAPEVTPTGVANPGGNSGGLIRDRLGRLM
                     RVVRRLGQPEQLADLTWRVGYRWGWRQAKLDDVVQLHPDDFRWSRSRIR"
     gene            complement(2844..3791)
                     /locus_tag="PKJ61_07015"
     CDS             complement(2844..3791)
                     /locus_tag="PKJ61_07015"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOC13612.1"
                     /translation="MEISESRDVAEVPSMQAPRVTVIIPVYNDLARLRTCLDLLATQT
                     YPRELLDVIVADNASSIDLSSALPPADERFRMIQEAKRGSYAARNAALGLASGDVVAF
                     TDADCLPHLDWIEVAVAALVAPDAPDAVGGAIRLVFRDGPDPVTGPELYESVHGFDQR
                     HFVEGLGFAATANLVTRREMIELVGPFNADLQSGGDDDWGHRLRDAGGRLEYLATAVV
                     DHPSRPTWSDLTIKAIRIADGMAALSREQSFGADLAELARELRSGLGSWLTIWGKDWP
                     TTPGAKARYVAALSWVAVLRLVVRIRYRIRERLHRKSSG"
     gene            4021..6246
                     /locus_tag="PKJ61_07020"
     CDS             4021..6246
                     /locus_tag="PKJ61_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13613.1"
                     /translation="MRSPRAAVELSALGHPAYLGFCDANALAGCRAEAGDLAARFVEF
                     TSTRVAAACSAELLLIRREHAALFWAVSDLRDLRFIAIERVSGAGGAVGRLAVAAARL
                     RGAARRLGRFSLGGSDFELFERPGCDPPRARTYFSPAWGPAGLAERLNAAGLRYALLR
                     WFEGLPHIEPGEDLDLLVADADAETLRGLVESEPGTQPIDLYSVSGLPASDYQDSAYY
                     PPGLARSLLERAVQHRSGFLVPEAMDHLHSLAYHAVYHKGERAGLRSEVLGPGVAGPE
                     HDYPAILAGLAKERGVDLPISYEGLDKYLSSVGWRPPTDTIRRLSAENPWAGRLIADG
                     DQPGAVLPGGAELAVFLVRERAVEVLGRNRILGVFDRFGFDVFHDEALPAAARNTAEQ
                     TLRGGNWGTGPFPTSGGAPSMLVVAVHHAPSQPYHWMAGRYPHLTNAETYEVKLALRG
                     LVEDLVEAPQWFNPVHSADNSTEAREYLRTVVPSLAADLESEAVGRAGALTAPSGMLR
                     NLSLGRRSRVDVVVAGGEHRVRKTFVPAFLRFRDREVDALRKLAGSVPALPELLATGP
                     NWFELPLYRDTLSHSAGHRLPMSVLRQMVAVLRQLYEAGYDVVDAKPANFLLDPELGL
                     KLVDLEFLYPYDGDRPAFAASANFGEPWPGFAGDRPVGDLSYESRWLPATGVPLSVLL
                     EGGAAATGWHRAISALRSATVAPGSSPRRVLRVAKSSADRLRAGARAAWQDFVRDQAG
                     L"
     gene            complement(6243..10709)
                     /locus_tag="PKJ61_07025"
     CDS             complement(6243..10709)
                     /locus_tag="PKJ61_07025"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HOC13614.1"
                     /translation="MKSGRLGLTVKLGAVALVAVASGVAVAFSGIGVPSFTPTPEPAE
                     SRDPAVIIASEADDARQIGGVSGQDVIPNFLTARRGPESTSALADSLWWSWTAPSDRK
                     MRFSTVGSEIDTAITVRVGGSSGTVVVAADRAGDLDTAEAVLDVVAAQTYSIEVSSEE
                     PEPMPGLVVLSWQPVASLGASVQASSAAPTATASGSATAGGTASTTTDSTGTESAAAA
                     TSDYAAAEIPLVGTTGEKPQSKLWRAHGSWWAVLASAAPSDAGTWVWRRDAGMWSAVL
                     RISDRTDVRADVLDTGSVVHVLLHGPTTTLVSLEYDGAANTYAWWADRPTPAAVSLPG
                     SETATIDVDSTGRLWLATETSSAIQVRWANAPYSTFSAAITVASGIDGDDISVVTKLP
                     GKIGVMWSNQTTKRFGFRTHTDSAAPGTWSTAEQVPATVLNLGGGVADDHLNVAVTTA
                     GKLYAAVKTSYNTSNKTVIALLVRSPDGTWDPLREVDTKGTRPIVLVDEDTNSLRVLY
                     TASEQLDDILSKTTRLDSVDFSADPEVVLPGAYNNVTSAKANVDGEAMILAGGATLTG
                     TVRLPWLDAQAPNVLNSVATTSVDTPLSANFNLGANSPAVDQVEVMESPVSGILTLTG
                     TTGFTYQPASGFTGVDSFTFRARSGSVWSGTATVRISVTTAPSLLGVWNLDETAGVSA
                     SDASGWQHDGQVSGAATWATGVTAGALRLDGSSGRVTIPHASELNATNTFTVATWVRP
                     EKVATQYLLKKADGSMTDGYELSLSSSGKPFFRVNQASSADSYRVNAPAVVPADGTTW
                     THLAGTFDGTRLRLYVNGVEVASVAGPSAIGQNNLALTFGGQPSGGGALQGSLDQIRL
                     YSRTLAAAEIAELAGAGTPTPTPTPTPTPTPTPTPTPTPTPTSTPTPTTPAETGSVGN
                     WAFDETSGTTALDSSGLANNGTLATGTTRTTGRNDGGALNFNGTTGQVTIPNHTSLNL
                     TTNLSVSAWVKPTKTATQYIIKKAQASTTNGYELSLASTGKPFLRINRATSADTYRVN
                     APNTIPADGTTWTHLAGTFDGNQLRLYVNGTLVATTNANTTIATNTLPLIIGAEPDNG
                     APFAGTIDDARLYNRTLTATDITNLYTTGTTATASPTTTPSPTPTPTPTPTPTPTPTP
                     TPTPTPTPTPTPTPTPTPTPTTPAETGSVGNWAFDETSGTTALDSSGLANNGTLATGT
                     TRTTGRNDGGALNFNGTTGQVTIPNHTSLNLTTNLSVSAWVKPTKTATQYIIKKAQAS
                     TTNGYELSLASTGKPFLRINRATSADTYRVNAPNTIPADGTTWTHLAGTFDGNQLRLY
                     VNGTLVATTNANTTIATNTLPLIIGAEPDNGAPFAGTIDDARLYNRTLTATDITNLYT
                     TGTTATSAAKSASVASSEPSGAAPASATPAAASSSAETAWAPASSASAQTSSAPAAPT
                     TQSPTPTATPSPTPKASQSAEESSAPPTVSASASETSDPATVSPTVTGASGP"
     gene            11003..12235
                     /locus_tag="PKJ61_07030"
     CDS             11003..12235
                     /locus_tag="PKJ61_07030"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOC13615.1"
                     /translation="MTNLRVVLVDHTSQDGGGELAALRVAERLHPDSVDIRVISFADG
                     PMVGRYAEIGVSVQLVPLGASANSISRKGLLTPRGLVTGITGVVRFLPSLVAALRRAR
                     ADLVVANSLKAAITVAIAAPLARLPWVWHVHDRLAPDYLPGGLSPLMRCLARLGPRSI
                     VANSRATLATLPPRARDRAVVAYPGVAATVTAAAGRSGATPLVGMLGRISPTKGQREF
                     ILAADAVASVFPEARFQVIGTALFGESDYEASVRRLARTLAVSDRIEFTGWLEHPSAR
                     LAELSLLVHASGVPEPFGQVVVEGMAAGVPVIATAAGGVPEILDPEGVADLGGRGFAV
                     TPFGILVRPADVGALAGALRHSLQDPDAARRRAERALVMVAQRFSIEQTVSTIEDAWL
                     RSLGRGPQSAGDPTRTER"
     gene            complement(12199..13239)
                     /locus_tag="PKJ61_07035"
     CDS             complement(12199..13239)
                     /locus_tag="PKJ61_07035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOC13616.1"
                     /translation="MRVIHAVRSDGFAGVERHVCVLAAAQVARGHDVTVIGGDERRMA
                     AAIGSADVTVLPGNTVASVALALSRTSPGADLIHAHMTAAELAAAVAALRPGPNLPLI
                     STRHFAARRGRTRLGRLAGRFIESRLAAQIAISQYVADHIEGGSEVVYPGVMDNPGSE
                     RPRSWTVLVVQRLEPEKRTDVALSAFAASKLADAGWRLQVVGDGSQREALQEQAARSG
                     IAPAVDFLGSRDDVAAQMTAAGLLIAPCPIEGLGLVVLEAMAAALPVVATAAGGHLET
                     LPPEAHDLCFEPDDVGAAATSLRLLAGDAGLRAHLGRLGHERQREKFTPQVQAEATDR
                     VYRSVLVGSPAD"
     gene            complement(13236..14441)
                     /locus_tag="PKJ61_07040"
     CDS             complement(13236..14441)
                     /locus_tag="PKJ61_07040"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOC13617.1"
                     /translation="MTESNASSRPPETLPPILQIAPEIAPGSGVGGVAYQLEQQFLAA
                     GVAVSRFTLAEARGAWLPEPGPGLRGRLALLFRVVWFSTIGTWLARRALAAAPTSISI
                     CHNDVLAGDIYVNHGILRAAMKARGHYLLRMLRNPLHLFTALRDAHRFRSRIHRVVVS
                     LTTREAELLSNTYSKVSAESVVIANGVDIDRFTPPPAESRLAARAEVLPNPNPAATWL
                     LFVGHEFDRKGLPLAIAALSQLSEPVGLLVVGGTPEMVVAAREQAVRAGVSEQVHLLG
                     RLADPMPAFRAADCLVLPSAYEANALVVLEALAAGLPAIVTPVGYAPDLIRDGVNGFL
                     VERTAAGVRQGIENYLRSDRRRLAEGARTSGEAHAWPLIAARYLELITRLAPDTRAPG
                     STDGKRTTT"
     gene            14593..16599
                     /locus_tag="PKJ61_07045"
     CDS             14593..16599
                     /locus_tag="PKJ61_07045"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOC13618.1"
                     /translation="MTQLEGQRPRVLRFAHHGVVAAWRERERELVRSGVDLTLVSARR
                     WNEGGTLIALDAGADDFVVGVDTLGSHPNGFVYDPRAVWRLLDREWDLIDLHEEPCAT
                     QTAEILAMLRLRGVRAPVLLYSAQNIPKRYPVPIRWLERYALRRAAGAYVCNAEAGRI
                     LQAKGMRAQPAVIGLGTDLEVFSPDSRTEPGSPLLVGYSGRLESHKGVDVLLKAIARV
                     PSTRLLLAGSGPQRPELERLASELGVSKRVDFLGHLDSGLADFYRQLDVLVVPSLPTP
                     GWLEQFGRVVVEAMASGAPVIASRSGALPEVIGPAGIVVEPNQPGEIAAALRELTEPR
                     RWRELRAAGLDHCRQYSWQAVALEHRALIDQVLATSAAGRGVGTAAAEDGAEDARPAH
                     PEVVIVAYGPAEKLAPSLQAVAGLSVTVVDNSSSEATRRLVEAHGAEYLDPGVNLGFA
                     AGVNHALAYLIRCGRDHADVLLLNPDATVSRDGVVELARRLHAEPGIACVAPRQTAPD
                     SDQPDRVEWPFPSPLGAWLTAVGLGAWDRGHTFLIGSILLLNAAALRDVGEFDERFFL
                     YAEETDWQRRAVTRGWRSQYVPSVTGSHVGAGTSSDESTRLRYFHSSQLAYARKHYGR
                     GGELLVRAAAIFGAAVRGVLKSGREREYARWRLRFYLTAPSARS"
     gene            complement(16574..17872)
                     /locus_tag="PKJ61_07050"
     CDS             complement(16574..17872)
                     /locus_tag="PKJ61_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOC13619.1"
                     /translation="MKWGRVLTGVALVLLVAGALGILSADYGSLAIFVAVIILALGLA
                     STDLVLLPVLAFPATLVMIRTGPLSVSDLVLIVATVPALMLYRRTEAKGLQPLLWAGA
                     VYLACLVPTLVLNRYLGNYIEWAHELILVLGSLLVGWVTGRAGRAGAALLAFQLGCVG
                     IGVAATLQALYLLRTTGVFGPAYLEFLHKNFIGNTLAFSFLIALVRPRWLNWPTWFHL
                     FVMVVSVLGIAASNSRQAVVSVFAGVAFLALRSWGRGATRRGRVLLLLLIPAVIATAG
                     TVSEQLESGDAFNSTSQRLTWYAESLRIWQQSPLFGVGLRWWNTGDHPAFQPPNAFFE
                     MISSAGVLGLFGFLVLCIAGLWVVLLLPRQYADLAAVVMIARFVQGQLDLYWVAGQSA
                     LPWMMTGLMIGLQALHQAQDPTTRGFASELPPLTTARRAR"
     gene            complement(17916..18548)
                     /locus_tag="PKJ61_07055"
     CDS             complement(17916..18548)
                     /locus_tag="PKJ61_07055"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13620.1"
                     /translation="MHPPSLSPAPFEAGPAALKLVFVCTANLCRSPIAELLAAREGRE
                     RGFPVLASSMGLMPGGRPSPALGVRVAAEFGLDLSGHRSRQLGLAELQSADLILTMTR
                     EHAREVVHLDPGLWPRVFTLKQHTEYLRRHPPNGSTVQDWMATVGPVRRRSELVGRYP
                     RHDVIDPMGRSAGVWREVVIDLSDHLSLLFDALVAARAGANANAQLATGA"
     gene            18852..19844
                     /locus_tag="PKJ61_07060"
     CDS             18852..19844
                     /locus_tag="PKJ61_07060"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOC13621.1"
                     /translation="MHIGQKPGLGRGRLGVPGTDGRLHRLELTKWLPRAAGPMADLLL
                     QRGVREAAAAIGMRKPLLWVNDPGWAGLVAATNWPAIYDITDDWVAADRPQREHDRIV
                     ANEAILMQGCREVVVCSEQLKRTKSAMRPVELVPNAVNLAWYRDPQPRPEDLPRGQIA
                     LYAGTLHEDRLDVDLAVVLGQALSRVGAQLVFVGPNALGSQNTDRLAAISGVHLLGSR
                     PYRDLPAYLQHADVLVVPHLVNDFTDSLDPIKLYEYQAAQRLIVSTPVAGFRQLAGLP
                     GVAICEARDFPAGVVDRLQRPPESIGPFDVADWDDRVVAMERVLSRARAPRDNG"
     gene            complement(19841..21568)
                     /locus_tag="PKJ61_07065"
     CDS             complement(19841..21568)
                     /locus_tag="PKJ61_07065"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HOC13622.1"
                     /translation="MTLRQVLEIIQRRWLLVALTTLVVLGSAAAYLQLTPEVYKSSSV
                     VRYSLTATRMFDGGSGYADTDLDLDPDFLTSPALIEIASPLTKDEPSALQAAIDTTLV
                     EGVRSNRLEIVAIASSAEQAQLRAQVVARVYVDHLAAQVKATQERLQTELTEAKAERT
                     EAVRQATKKPNDPVARQRLETALGNVSIIEDGLVSIANSGPPASILQSALPGDRTGTD
                     ALTVLLIGLASGLLAGAGIALIRDLFDDRLRGAVAADDDLGIPVLAELTRIPRRHRYD
                     RTLPTTTTRSAPFHEGIRALRTSLNVLYPEPHSAIAITSAEPGDGKSFITANLAVSMA
                     RGGRSVIVVGADLRRPTLDHYFGLAPTRGIADAIVDGLDAASLEKLLQPTAYERLRVL
                     SAGIARREPADLLAADAFKLVVRQLRQFADVVIFDTPPGLALADAAVIGAQVDGTVVV
                     AAAKSTTHRRLAETVRILAGNGVEIAGAVVNRSHKRATRAYTHYYGTTHPEDDFDQDP
                     LDVARLEAPTPPTWKPSQQPESADASPATPPASPRSPEPDQNPDAAQGSPAPGRIPGR
                     AKPAARPGT"
     gene            complement(21706..>22404)
                     /locus_tag="PKJ61_07070"
     CDS             complement(21706..>22404)
                     /locus_tag="PKJ61_07070"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HOC13623.1"
                     /translation="AAVLRPRMGGGAIVARERDLALSNFDDLSDLAAFVQLPESDRMR
                     CNDGGCDPDGAFWIGTLAYSFEAGAGNLYQLDAGSRRPHRVLDGLSISNGIGWSPDAT
                     TMYLNDSGTGLTWAFDYDPIEGVSGRRVFAEAADDGLPDGLCVDAEGGVWTARYGGGQ
                     VNRYSPSGTLEAVIEVPGVSKVTACCFGGPELDTLYLTTSRENLDAGAEPAAGSVFAV
                     EPGVVGLPVLGFAG"
BASE COUNT         3512 a         7101 c         7990 g         3801 t
ORIGIN      
        1 aattccaggc cgaccggcaa tcagaccgga cggcacaccg acggattgtt cagcaagctc
       61 ctagagccga acccgttcgt cgaccctgga tacctgcttc cggcattgcg gtgggacggc
      121 cagggtgccg ggatcaagct ggtggttgtc atggacgggg aggattggat agccgccctg
      181 ccattgtcgg tgatgccgcc cgcgtggaag cttccattgc gcctggccac caccgccgga
      241 cccttcctct ctcggcgggc accgctgtgc gcgcccctgg tcgaccgggc gcgtgccgag
      301 ccggccatcg aaaggctgat ggagcatctg gcctcacctg cgtccggtct ccccgggctg
      361 gtcgaactca cgctgttgcc ggtcggaaga ctgaccgagc tgatccgcgc cgaatgcgac
      421 cgcaaacggg tgcggtgcct cgagcgctac cgcttcgagc gcgcgtgcct ggtgcgatcc
      481 gcggggaagt cgccgcgtgg cagcctgagc gttcgccgac gcaaactact cgcccggaag
      541 gaacgtggtc tggagcgcag cttgggcggt ccgctcgaac tggtcgacct cggctgcgac
      601 caggcggcgg tcgatctctt cctcgacctc gaagcgtccg gctggaaggg tgctcgcggc
      661 agcgccctgc gccgcaagcc cggggcggct gcctggttca acgaggtgtc ggccggcttc
      721 gccgagcgtg ggatgctgcg cattctggcg ttgcaggccg gtggtgagac ggtctattca
      781 gcagtccagt tgctcgtcgg caatacggtc ttcggcatga tggacgcgta cgaagagcgc
      841 tggtccgcct tcaccccagg cgtgatcgga cgcgtccgag aacaggagga attcatggat
      901 cgcggcagcg ccgagctctt ggatccgtgt atccacccgc gctacgtaca gccctctgcg
      961 ctctaccccg accgccgcct gatcgtcggt ttggtgttgg cgccaagggg cctgatcaac
     1021 tcccagttcc tccggctacg accgggcatg atcctcgtcc gggacaagta cctgaaggtg
     1081 gctgcggcag tctccgggtg gatgcgccgt ggctagtcga ggccaggccg tcctgctggt
     1141 aaggcgaggc gacattgaag cgcgacccga ccgaccggtc ggcctggaag ctgcggcgcg
     1201 gggacgcggc cggttggcgg ctgtcgtggg ccgcagtctg gcaaccttct gcgtgagtac
     1261 gaccgagagc gtttttggac tgagctacga cgatggcccc gacccggagc agacgtcggc
     1321 cattctcgat gtccttgccc ggcactgcgc gcatgcgacc ttcttcgtgc tcgccggacc
     1381 ggcagcgaag caccccgaga tactgcgcag aatggtggcc gaaggacacg aagtcgcact
     1441 ccacggactg gatcatgctt cgctcctcac tcgaagcact tcggcggcgg tcgcagcggt
     1501 cctcgaagca cgtgcggtcg tggagtccat tgtcggcacc tcagtacgtc actaccgccc
     1561 gccttacggc gcccacacgc tgaggcaggc acgggccatc caccgggcgg ggctcgaact
     1621 agtggtctgg tccggcgatg ccaaggactg ggtgcaggct gacgaggatg tcatcattaa
     1681 gcgcgcgtgg gcgacggtgt tcccgggcgc cttcctgttg ttgcacgaca ccagggccga
     1741 cccggagacc gcgctggacc cgagtgaact gccgaccttc gaccgagctg ccgtgctgga
     1801 ggatctgctt cttcgggcaa ccgcggccgg ctactctgca ctcaccgtcg gggatctcat
     1861 ggacaggttc cccgctgtga agagctgggc gagagaacgc agccggatgt gaccgacacc
     1921 gagtccatcc ccgaactcag cgtagtgatc gccgctttca acgctggcgg caccttgggg
     1981 gaacaactcc gggagttggc cgcccaaacg ccgcagtggc cctgggaggt gatcgtcgcg
     2041 gacaacggat ccaccgatcg gacgaccgaa gtggtcgccg agttgcaggc cgactttccg
     2101 tggttgcggc tagtggacgc atcgcagcgg cgcggccccg ctgcggcgcg taacgtcggg
     2161 gtgacgtacg cgcgagccgc ccggatcgcc ttctgcgacg ccgacgactt gattgccgac
     2221 ggctgggtcg agacgatgca cgccgcgctg gtggagcacg agttcgtggc gggttccttc
     2281 gatacgaggc gactggctgg gcggaaccga ttcgtggtga gctggtctcc gcagcgggag
     2341 gcgctctcgg ttaagcccta cctccccgag ttcgtgaccg gcgggtccgg caatctcgga
     2401 attcgaagga gcgtcttcaa cgaggccggt ggtttcgatg aatcggcgat ggtcgctgag
     2461 gatgacgatc tctgcctgag ggtgcaattg gcaggtcacc agctggtgtt cgagtccagc
     2521 ctggttctgc acgtacgtcg tcgcgccggg cttagagccg tgcttcgtca ggcccgtgct
     2581 tacggagccg gtgcccggcg gttggagcat cggtatgcgc ggatcgtggc acggctgcat
     2641 cctgccgtcc cggcccctga ggtcaccccg accggtgtcg caaacccggg cggcaacagc
     2701 ggcggactaa tccgggatcg gctggggcgc ctgatgcggg tggtccgacg tttggggcaa
     2761 cccgaacagc ttgccgacct cacctggcgg gtcggttacc gctggggctg gcggcaggcg
     2821 aagttggacg acgtggtcca gcttcacccc gacgacttcc gatggagccg ctcacggatc
     2881 cggtaacgga tgcgcacgac caaccgcagg accgccaccc agctgagcgc ggccacgtag
     2941 cgagccttgg ccccgggggt ggtgggccag tccttccccc agatggtgag ccacgacccc
     3001 aggccggacc gtagctcacg ggcgagttcc gccaaatcag ccccgaacga ttgctcacgg
     3061 ctcagtgcgg ccatgccatc ggcgattcgg atcgccttga tggtcaggtc cgaccaggtg
     3121 ggcctcgagg ggtggtccac caccgcggtc gccagatact ccaaccgccc acccgcgtcc
     3181 cggagccggt ggccccagtc gtcgtccccg cccgattgga ggtcggcatt gaagggaccg
     3241 accagttcga tcatctcccg gcgggtgacg agattggccg ttgcggcgaa gccgagaccc
     3301 tccacgaagt gtcgctggtc aaagccatgg acagactcgt atagctcggg cccggtgacc
     3361 gggtcaggtc catcgcggaa cacgagccta atcgcgccac cgacggcgtc cggtgcgtcc
     3421 ggcgcgacca gcgcggccac ggcgacctcg atccagtcca aatggggtag gcagtcggca
     3481 tcggtgaagg ccacgacatc gccgctcgcg agtcccaggg cggcgttgcg ggccgcatag
     3541 gagccacgtt tggcttcctg gatcatccgg aaccgttcgt ccgcaggagg caacgccgac
     3601 gacaggtcga tactggaggc attgtccgcc acgatcacgt ccagcaactc tcgggggtaa
     3661 gtctgggtgg cgagcaggtc gaggcaggtg cgtaaccggg caaggtcgtt gtacaccgga
     3721 atgatcaccg tgacccgggg cgcctgcatc gacggtactt cggcaacgtc gcgtgactca
     3781 ctgatctcca tccggccccc tccccgcaac agatccggtc gcccgccccg atcggagctg
     3841 tactcccacc ggggctgcgg ctagcctagc tggcgacgtc catcttcgag ctcttcgctg
     3901 gcggtgacgg gcgggtttga tcgcggcggc ggacggggga cggtggccgg cgacagtcga
     3961 cttggcgcgc tgttgggcgc agactgtggc gcgggcagga tcagggtgat cgatcttggc
     4021 gtgaggagcc cgcgcgcggc agtcgagttg agcgccctcg gtcaccccgc ctatctgggg
     4081 ttctgtgacg cgaatgctct cgccggctgc agggctgaag ccggtgacct cgctgccaga
     4141 ttcgtcgaat tcaccagcac gcgagtggcg gcggcctgct cggcagagct tctcctgatc
     4201 cgccgagagc acgccgcact gttctgggcg gtatccgacc tgcgggacct ccgtttcatc
     4261 gcgattgaac gcgtctcagg cgccggcgga gcggttggcc gtctggcggt ggccgcagcc
     4321 cggcttcgtg gcgcagcgcg ccggttgggc cgattcagcc ttgggggcag cgacttcgag
     4381 ctcttcgagc ggccggggtg cgacccgccc cgggcgcgaa cctacttctc gccggcgtgg
     4441 ggacccgccg gcctggccga gcgtctgaac gcggcggggc tgcgttacgc gctgcttagg
     4501 tggttcgagg gcctaccgca tatcgaaccg ggcgaagatc tcgacctgct ggtcgccgat
     4561 gcggacgccg agacgctccg aggcttggtc gagtcagaac caggaaccca gcccatcgac
     4621 ctctacagcg tctccggtct gccggccagc gactaccagg attcggccta ctacccgccg
     4681 gggttggcgc ggagcctgct tgagcgggcg gtgcaacacc gcagcgggtt cctggtgccc
     4741 gaggcgatgg accatcttca ttccctcgcg taccacgcgg tctatcacaa gggggagcgg
     4801 gccggtcttc ggtctgaggt cctggggccg ggggttgccg ggcccgagca cgactacccg
     4861 gcgatcctcg ccggcctagc caaggaacgc ggcgtcgacc ttccgatcag ctacgagggg
     4921 ctggacaagt acctgagttc ggtgggctgg cgtccgccca cggacacgat ccggcggctc
     4981 agcgctgaga acccttgggc aggcaggctg atcgccgacg gcgaccagcc tggggccgtc
     5041 cttcccgggg gtgccgagct tgccgtcttt ctggtgcgcg agcgggccgt ggaagtgctc
     5101 ggacggaacc gcatcctcgg ggtgttcgat cgcttcggct tcgacgtgtt tcacgatgaa
     5161 gcgctgcccg ctgcggcaag gaacacggct gagcagactc ttcggggagg caactggggc
     5221 actgggccgt tcccgacgag cggcggcgca ccgtccatgc tggtggtggc cgtccaccat
     5281 gctcccagtc agccctatca ctggatggcc ggccgctacc cacacttgac caacgcggag
     5341 acctatgagg tgaagctcgc gctgcgaggc ctcgtggagg acctggtcga agcaccgcag
     5401 tggttcaacc ccgttcattc ggccgataat tcgaccgagg ctcgcgagta cctgaggaca
     5461 gtggtcccca gcctcgcagc ggacctggaa tccgaggcgg taggcagagc cggggctctc
     5521 accgccccgt ccggaatgct gcggaacctc tcgcttggtc gacgctcccg cgttgacgtg
     5581 gtggtcgccg gcggtgaaca ccgggtgcgt aaaaccttcg tgccggcgtt cttgcggttc
     5641 cgagaccgcg aagttgacgc tctacgcaag ctcgccggaa gcgttcccgc gttacccgaa
     5701 ttactggcga cggggccgaa ctggtttgag cttccgctct atcgggacac tctgagtcac
     5761 agcgccggcc accgattgcc gatgtccgtc ctgcgtcaga tggttgctgt gctccggcaa
     5821 ctctacgagg cgggctatga cgtagtggac gcgaagccgg cgaacttcct cctcgatccc
     5881 gaactcggcc tgaaactggt cgacctggag tttctctacc cttacgacgg cgatcgcccg
     5941 gccttcgctg cgagcgcaaa cttcggcgaa ccgtggcccg gtttcgccgg cgatcgccct
     6001 gtgggcgacc tttcatacga gtcacggtgg cttccggcga caggcgtgcc gctgtccgtc
     6061 ctcttggagg ggggtgctgc agccaccggt tggcaccggg cgatcagcgc gttgcggagt
     6121 gccaccgtgg cgcccggctc ctcacctcgc cgagtgttgc gggtcgcgaa gtcgagcgcg
     6181 gaccgcctac gcgccggcgc tcgggccgcc tggcaagact tcgtccggga ccaggccggg
     6241 ctctaggggc cggaggcgcc ggtcactgtg ggactgaccg tggccgggtc tgatgtctcg
     6301 gacgccgaag cactgaccgt aggcggcgca gagctttctt cagcgctctg gctggccttt
     6361 ggcgtcgggc tcggtgtggc tgtcggtgta ggcgactgtg tcgtcggggc tgccggggcc
     6421 gacgaagtct gggccgaagc cgacgaggcc ggggcccaag cggtctcggc agacgaggat
     6481 gccgcggcag gagtcgcgct cgctggagcc gctcccgatg gttcggagga cgccacgctc
     6541 gccgacttcg ccgccgaggt ggcggtggtg ccggtggtgt agaggttggt gatgtcggtg
     6601 gcggtgaggg tgcggttgta gaggcgtgcg tcgtcgatgg tgccggcgaa gggggcgccg
     6661 ttgtctggtt cggcgccgat gatgagtggc agggtgttgg tggcgatggt ggtgttggcg
     6721 ttggtggtgg ctacgagtgt gccgtttacg tataggcgca gttggttgcc gtcgaaggtg
     6781 ccggcgaggt gggtccaggt ggtgccgtcg gcggggatgg tgtttggcgc gttgacgcgg
     6841 taggtgtcgg ctgaggtggc ccggttgatg cggaggaagg gtttcccggt gctggccagg
     6901 gagagttcgt agccgttggt ggtggaggct tgggccttct tgatgatgta ttgggttgcg
     6961 gtcttggtgg gtttgaccca ggcgctgacg ctgaggttgg tggtcaggtt gaggctggtg
     7021 tggttgggga tggtgacttg gccggtggtt ccgttgaagt tgagggcgcc gccgtcgttg
     7081 cgtccggtgg tgcgggtggt tccggtggcc agggttccgt tgttggccag gcctgagctg
     7141 tcgagggcgg tggtgccgct ggtttcgtcg aaggcccagt tgccgaccga gccggtctcg
     7201 gccggagtcg ttggcgtggg tgtaggggtt ggcgtgggcg tgggcgtcgg ggtgggtgtc
     7261 ggcgtaggcg taggggtggg tgtgggggtt ggcgtcgggg tgggtgtcgg cgtaggcgta
     7321 ggtgacggcg tagtcgttgg ggaagccgtg gcggtggtgc cggtggtgta gaggttggtg
     7381 atgtcggtgg cggtgagggt gcggttgtag aggcgtgcgt cgtcgatggt gccggcgaag
     7441 ggggcgccgt tgtctggttc ggcgccgatg atgagtggca gggtgttggt ggcgatggtg
     7501 gtgttggcgt tggtggtggc tacgagtgtg ccgtttacgt ataggcgcag ttggttgccg
     7561 tcgaaggtgc cggcgaggtg ggtccaggtg gtgccgtcgg cggggatggt gtttggcgcg
     7621 ttgacgcggt aggtgtcggc tgaggtggcc cggttgatgc ggaggaaggg tttcccggtg
     7681 ctggccaggg agagttcgta gccgttggtg gtggaggctt gggccttctt gatgatgtat
     7741 tgggttgcgg tcttggtggg tttgacccag gcgctgacgc tgaggttggt ggtcaggttg
     7801 aggctggtgt ggttggggat ggtgacttgg ccggtggttc cgttgaagtt gagggcgccg
     7861 ccgtcgttgc gtccggtggt gcgggtggtt ccggtggcca gggttccgtt gttggccagg
     7921 cctgagctgt cgagggcggt ggtgccgctg gtttcgtcga aggcccagtt gccgaccgag
     7981 ccggtctcgg ccggagtcgt tggcgtgggt gtagaagtcg gcgtgggcgt aggcgtaggg
     8041 gtgggtgtgg gggttggcgt tggcgtgggt gttggcgtcg gggtcggcgt ccccgcgccg
     8101 gccagctccg cgatttccgc ggcagctagg gtgcgggaat agagccggat ctggtcgagg
     8161 ctgccctgca atgcgcctcc gccgctcggc tggccgccga aggtgagggc gaggttgttt
     8221 tggccgatcg cgctcggccc ggcaactgaa gccacctcga caccattcac gtataggcgc
     8281 agccgggtgc catcgaaggt gccggcgaga tgcgtccacg tcgtgccatc ggccggaacg
     8341 acggctggcg cgttcactcg atagctgtcg gcggaactcg cctgattcac cctgaagaag
     8401 ggcttgccgc tgctggacag cgagagctcg taaccatcgg tcatcgagcc gtccgccttc
     8461 ttgagcagat attgggtggc gaccttctcg ggtcgaaccc aggtggcaac cgtgaaggtg
     8521 ttcgtagcgt tgagttccga ggcatgcggg atcgtcaccc tgcccgacga cccgtccagg
     8581 cgcaaggctc cggcagtcac acccgtggcc caggttgcgg caccggacac ctgcccgtca
     8641 tgctgccagc ccgacgcgtc ggacgccgag acgcctgcgg tctcgtccag gttccatacc
     8701 cccagcagcg aaggtgccgt tgtcaccgag atccgaaccg tggccgttcc cgaccagact
     8761 gatccactgc gtgcgcggaa ggtgaaggag tcgacgccgg tgaagcctga tgccggctgg
     8821 taggtgaagc cggtggtgcc ggtgagcgtc aggatccccg aaaccggtga ttccataacc
     8881 tcgacctggt ccaccgcggg ggagttggcc ccgagattga agttggccga gagcggtgtg
     8941 tcgacactgg tcgtagcaac ggaattcagc acgttgggag cctgggcatc gagccagggc
     9001 aggcgcacgg taccggtcag tgtggcgcca ccggcgagga tcattgcctc gccgtcgacg
     9061 ttggccttgg cactcgtgac gttgttgtag gcgcccggga gcacgacctc gggatcggcc
     9121 gaaaagtcga cggaatcgag tcgggtcgtc ttggacagga tgtcgtcgag ttgttcgctc
     9181 gcggtgtaga gcacccgcag cgagttggtg tcctcatcga caagaacgat cggtcgagtg
     9241 cccttggtgt cgacctcgcg aagcggatcc caggtgccgt ccgggctgcg tacaagaagc
     9301 gcgatcaccg tcttgttgga ggtgttgtac gaggtcttga cggccgcgta gagcttgccg
     9361 gcagtggtca cggcgacgtt gaggtggtcg tcggccacgc cgccgcccag gttgagcacg
     9421 gtggccggta cttgctcggc tgtcgaccag gtgcctgggg cggcggagtc ggtgtgcgtg
     9481 cggaatccga accgcttcgt ggtctggttt gaccacatca cgccgatctt gcccgggagc
     9541 ttggtcacaa ccgagatgtc gtcgccgtca atccctgagg ctacggtgat ggcggcggag
     9601 aaggtgctgt acggagcgtt ggcccaacgt acctgaatgg cggacgaagt ctcggtagcc
     9661 agccacaacc gaccggtgct gtcgacgtcg atggtcgcgg tctcgctgcc cggcagggag
     9721 accgcggctg gagtcgggcg atccgcccac cacgcgtagg tgtttgcagc gccgtcgtac
     9781 tccaaagaca cgagagtggt cgtcgggccg tggagcagaa cgtggacgac cgagcccgta
     9841 tcgagcacgt cggcgcgaac atcggtgcga tccgagatgc ggaggacggc agaccacatg
     9901 cccgcgtcac ggcgccaaac ccaggtgccg gcatcggacg gggccgcgga agcgagcact
     9961 gcccaccagg agccatgcgc tcgccacaac ttggactgcg gcttctcgcc ggtcgtcccg
    10021 accagaggga tctcggcggc ggcgtagtcg ctcgttgctg ctgcggcgga ttcggtgccg
    10081 gtcgagtcgg tcgtggtcga agccgtcccg cccgcggtcg cagaccctga agcggtcgcg
    10141 gtgggtgccg ccgaactcgc ctgcacactc gctccgaggg acgcgacagg ctgccaggaa
    10201 aggacgacca gacccggcat aggttctggt tcctccgagc tgacctcgat ggagtaggtc
    10261 tgcgcggcca caacatccag cacggcttcg gccgtatcga ggtcgccagc ccgatcggcc
    10321 gccactacga cggtgccgct ggagccgccc acccggacag tgatcgcggt gtcgatctcg
    10381 ctgcccacag tggagaaccg catctttcgg tcactgggcg ccgtccagga ccaccacagc
    10441 gagtctgcca acgcggatgt ggactccggg cctcggcggg cggtgaggaa gttcggaatt
    10501 acgtcctggc cggagacgcc accgatctgg cgcgcatcgt ccgcctcgct ggcgatgatg
    10561 acggcaggat cccgcgactc ggcgggctca ggagtaggcg tgaaggatgg aactccgata
    10621 cccgaaaacg ccactgccac gccggacgcg acggcgacaa gggcgaccgc accgagcttg
    10681 acggtgaggc cgagacgtcc ggacttcatg atgagaaccg gctggggcgg tcgccaactg
    10741 ctggcggacg gcgcgagaac gcgcccggct gctgataatc gagactggtc atgcttcccc
    10801 cgtacagacc aagaccgatc cccccccgag accggactgc tgaagcggag ctcccgacgc
    10861 tccggtccaa ctagccgcag tataggccgt tctggagcgc tcttgcaggg cccgacccaa
    10921 cgaatgggtc cgggaatccc gcctcagtgg ctgctgaagc gtgggatcat tagcgaatgc
    10981 gggcgcgccc gaaaggagcc ggatgacgaa tcttcgggtc gtgctggttg accacacgtc
    11041 ccaggacggc ggcggagaac tcgcggcgct gcgggtcgcg gagcggcttc acccggactc
    11101 ggtcgatatc cgagtaatca gttttgctga cggtccaatg gtgggccgct acgccgagat
    11161 cggtgtttcg gtgcagttgg tgccactcgg tgcgtcggcg aacagcatct ccaggaaagg
    11221 cctactgacc ccgcgaggct tggtcaccgg aatcaccggc gtggtgcgct tcctgcccag
    11281 cctggtcgct gcgctgcggc gcgcgcgcgc cgacctagtc gtggccaact cgctgaaggc
    11341 ggccatcact gtggccatcg cggcgcccct ggccaggctg ccgtgggtct ggcacgtgca
    11401 cgaccggctc gcaccggact acctcccggg cggactttct ccgctgatgc gatgcctggc
    11461 tcggctgggg ccgagatcga tcgtcgccaa ttcccgagca accctggcca ctctcccgcc
    11521 ccgggcgcgc gaccgcgcgg tcgtcgccta ccccggagtc gccgcgactg tcaccgccgc
    11581 ggcaggacgc tccggggcga cgccgctggt gggcatgctc ggacggatca gccctaccaa
    11641 gggacagcgg gagttcattc tggcggcgga cgcggttgcg tccgttttcc ctgaagctcg
    11701 cttccaggtg attggaaccg cactcttcgg agagtccgac tacgaggcat cggtccgccg
    11761 gctcgcgcgg acgctcgcgg tcagcgaccg gattgagttc acgggttggc tggagcaccc
    11821 ctcagcgcga ctggcggaat tgtcactgct ggtccatgcc tccggggtgc cggagccctt
    11881 cggtcaggtg gtggtggagg ggatggctgc cggagttccg gtgatcgcca cagcggccgg
    11941 tggcgtacca gagatccttg accccgaagg cgtcgccgat ctaggcggtc gggggttcgc
    12001 agtcacaccc ttcggcattc tcgtccgtcc ggccgacgtc ggcgctttgg cgggtgccct
    12061 tcggcacagc ctgcaggacc ccgacgcggc gaggcggcgg gccgagcgcg cgctggtgat
    12121 ggtggcgcag cgattctcga tcgagcagac ggtgagcacg atcgaggacg cctggctgcg
    12181 atcgcttggc cgcgggcctc agtcggcagg tgacccgacc aggaccgagc gatagacccg
    12241 gtcggtagcc tcggcttgaa cctgaggcgt gaacttctcc cgctggcgtt cgtggccgag
    12301 cctccccagg tgagcccgca agccggcgtc gccggcgagc aggcgaaggg acgtggccgc
    12361 cgcgcccaca tcgtcaggtt cgaagcagag gtcgtgggcc tcgggtggca gggtctccag
    12421 gtggccgccg gcggccgtcg ccaccaccgg taacgcggcg gccatggctt cgagtacgac
    12481 cagtcccaac ccctcgatcg ggcaaggagc gatgagcaac ccggctgcgg tcatctgggc
    12541 ggcgacgtcg tcgcgagaac ccaggaagtc cacggccggc gcgatcccgg accgggcagc
    12601 ctgctcctgc agggcctccc gctgcgaacc gtccccgacc acctgcagcc gccagcccgc
    12661 gtccgcgagt ttggaggcgg cgaacgctga cagggcgacg tcggtgcgct tttcgggctc
    12721 cagtcgctgc accaccagca ccgtccaact ccgcgggcgt tcagatccgg gattgtccat
    12781 cacacccggg tagaccacct cactgccccc ctcgatatgg tcggcgacgt attggctgat
    12841 cgcgatctgc gcggcaagcc gggactcgat gaaacggccc gcgagtcgac cgagccgcgt
    12901 ccgtccgcgc cgggccgcga aatgcctcgt gctgatgagc ggtaggttcg gcccagggcg
    12961 caatgccgcg actgccgcgg ccaactcggc tgcggtcatg tgagcatgga tcaggtcggc
    13021 cccgggcgag gtacggctga gcgccagcgc caccgatgcg acagtgttcc ccggcaagac
    13081 cgtgacgtcg gcggagccga tcgcggcagc catccgacgc tcgtcgccgc cgatcacagt
    13141 cacgtcgtga ccgcgcgcga cctgagctgc ggctaggacg cagacgtgtc gctccacacc
    13201 cgcgaagcca tctgagcgga ccgcgtggat caccctcacg tggtcgtcct cttcccgtcc
    13261 gtcgatccag gtgcccgagt atccggcgcg aggcgggtga tcagctccag gtacctggcc
    13321 gcgatcagtg gccaggcgtg cgcttcgccg ctggtgcgag cgccttcggc cagtcttcgt
    13381 cgatcagagc gcaggtagtt ctcgatgccc tgacgcaccc cggccgccgt ccgctcgacc
    13441 aggaatccgt taacaccatc gcgaatcagg tctggggcgt atccgaccgg cgtcacgatc
    13501 gcgggcaagc cggcagccag cgcctccagc acgaccaggg cattggcttc gtaggcgctc
    13561 ggcagcacta ggcagtccgc ggcacggaag gcgggcatcg ggtcagcgag gcgtccgagc
    13621 agatggacct gctcggacac ccccgccctg acggcttgct cgcgtgccgc aaccaccatt
    13681 tccggggtgc cacccacgac gagcaacccg accggttcgc tgagctggga tagcgccgca
    13741 atcgctaggg gcagcccctt gcggtcgaac tcgtggccga cgaagagcag ccaggtggcc
    13801 gcaggattcg ggttcggcag cacctcggct cgggcggcga ggcgggactc ggccgggggc
    13861 ggggtgaagc gatcgatgtc tacgccgttg gcgatcacca ccgactcggc cgacaccttc
    13921 gagtaggtgt tggagagtag ctcggcctcg cgcgtggtga ggctcaccac cacccggtgg
    13981 atgcgcgagc gaaaacggtg ggcgtcccgc aacgcggtga acaggtggag cgggttccgc
    14041 agcatccgca gcaggtagtg gccccgggcc ttcatcgctg cccgcaggat gccatggttg
    14101 acgtagatgt cgccggcgag gacgtcgttg tgacagatcg agatcgaagt gggcgcggct
    14161 gcgagcgcgc gccgagccaa ccaggtgccg atcgtggaga accagaccac gcggaagagc
    14221 agcgccagcc gcccgcgtag gcccggcccg ggctcaggca accaggcacc cctggcttcg
    14281 gccagggtga accggctcac cgccacgcct gcagcgagga actgctgttc cagctggtag
    14341 gccacgccgc cgacgccgct gccgggcgcg atctcgggcg cgatttggag gatcggcggc
    14401 agggtttcgg gtggccggct actggcgttg ctctcggtca tggcacccct tctggcgaac
    14461 ccgccgagat cccgaaagag cggtcgcgct tccgcgagct atgctgccgc aatctggggg
    14521 gagccgctag ccacgacggt cgcccgcgag cggacgtatt ccccacgctg atgccatcgg
    14581 ggggagtggg gcgtgacgca gctggaagga cagcgaccgc gggtgctcag gttcgcccac
    14641 cacggcgtcg tcgctgcctg gcgcgagcgg gaacgcgaac tggtgcgctc aggcgtggac
    14701 ctcactttgg tttcggcgcg gcggtggaac gagggcggca ccctgatcgc gctggacgcg
    14761 ggtgctgacg acttcgtggt cggcgtggac acgttgggtt cgcacccgaa cggcttcgtg
    14821 tacgacccac gtgccgtctg gcggctgctt gatcgcgagt gggacctgat cgaccttcac
    14881 gaagagccgt gcgccaccca gactgcggag atcctggcga tgcttcggct gcgcggggtt
    14941 cgggcgccgg tgctgctcta ttcagcgcag aacattccca agcggtaccc ggttccgatt
    15001 cgctggctgg agcgctatgc cctgagacga gccgcgggcg cgtacgtatg caacgcagag
    15061 gccggacgga tcctgcaggc gaaaggcatg cgcgcccaac ccgcggtgat cgggctcggg
    15121 accgatctgg aggtattctc cccggacagc cgcacggagc cgggctcgcc gctactggtc
    15181 gggtactccg gccgactgga gagtcacaag ggtgtcgatg tcctgctcaa ggcgatcgca
    15241 cgcgtcccat ccaccaggct gttgctcgca gggagtgggc cgcagcggcc ggagttggaa
    15301 cggctggcat ccgagttggg ggtctccaaa cgggtcgact tcctcggcca tctcgactcc
    15361 ggactcgcgg acttctatcg ccagctggac gtgctagtgg tgccatcgct gcccaccccc
    15421 gggtggctgg agcaattcgg ccgggtcgtg gtggaggcca tggcctcggg ggccccggtg
    15481 atcgccagcc gcagcggtgc gttgccagag gtcattggcc ctgcgggtat cgtggtcgaa
    15541 cccaaccaac ccggcgagat tgccgcagcg ctcagggagc tgaccgaacc tcggcgctgg
    15601 cgtgaacttc gcgccgccgg gctggaccac tgccgtcagt acagctggca ggcggtcgcg
    15661 ctggagcata gagccctaat cgaccaggtg ctcgcgacct ccgccgccgg ccgcggcgtc
    15721 gggaccgctg ctgccgaaga tggcgctgag gacgcccgtc ctgctcaccc cgaggtggta
    15781 atcgtcgcct acggcccagc cgagaagctg gcaccctcgc tgcaggcagt ggccgggctg
    15841 agcgttactg ttgtcgacaa ttcttcttcg gaagcgactc gtcggctggt cgaggcgcac
    15901 ggcgccgagt atctcgaccc gggtgtgaac cttgggttcg ccgcgggggt taatcacgct
    15961 ctggcatacc tcatccgatg cggacgtgac cacgcggacg tcctgctgct caacccggac
    16021 gccaccgtct cccgggacgg ggtcgtcgag ctggcgcgcc ggctgcacgc cgagccaggc
    16081 atagcctgcg tcgctccgcg gcagactgct ccggattcgg atcagccgga ccgggtggaa
    16141 tggccatttc ccagtccctt gggtgcctgg ctcaccgcag tcggactcgg cgcctgggac
    16201 cgtggtcaca ccttcctgat cggatcgatc ctgctgctga acgcggcggc actgcgcgac
    16261 gtgggggagt tcgatgagcg cttctttctc tatgccgaag agaccgattg gcagcgccga
    16321 gcggttaccc gcggttggcg gagccagtac gtcccgtccg tgaccggcag ccacgtcggc
    16381 gccggaacca gttcggacga gtcgacgcgc ctgcgctact tccacagctc ccagctcgcc
    16441 tatgcccgca agcattacgg ccgcggtggt gagttgctgg tgcgcgctgc ggcgatcttc
    16501 ggggctgctg tgcgaggggt gctcaaatcc ggacgcgaac gcgaatacgc ccgctggcgg
    16561 ctgcgcttct acctcaccgc gccctccgcg cggtcgtaag cggcggcagt tccgaagcga
    16621 acccacgtgt ggtcgggtcc tgcgcctgat gcagcgcctg cagtccgatc atcaagccgg
    16681 tcatcatcca tggcaacgcc gattgcccgg ccacccagta gagatcgagt tggccctgga
    16741 cgaagcgcgc gatcatgacg accgcggcga ggtcggcgta ctgccgggga agtagcagca
    16801 ccacccacag tccggcgatg cacagcacca agaagccgaa caggccgagc acgccggccg
    16861 atgagatcat ctcgaaaaac gcgttggggg gctgaaaagc tggatggtca ccggtgttcc
    16921 accatctcag tccaaccccg aacaacggcg attgctgcca gatccgaaga ctctcggcgt
    16981 accaggtcaa gcgctgcgac gtggagttga acgcgtctcc cgactccaac tgctcgctca
    17041 ctgtgcctgc ggtggcaatc acagccggga tcaggagtag caacagcacc cggccgcgtc
    17101 gagtggcgcc gcgtccccaa ctgcgcaggg ccagaaaggc gacgccggcg aatacggaaa
    17161 cgacggcctg ccgggagttc gaggcggcga tcccgaggac actgaccacc atcacaaaga
    17221 ggtggaacca ggtgggccag ttgagccacc ggggccggac cagggcgatc aggaacgaga
    17281 acgcgagggt gttgccgatg aagttcttgt ggagaaactc cagataggcc ggtccgaaaa
    17341 ctccggtggt tcgcagtagg tagagggcct gcagcgtcgc agctactccg atgccgacgc
    17401 aaccgagctg aaaagccaac agggccgctc cggcccggcc ggcgcgtccc gtcacccagc
    17461 cgaccaggag gctgcccagc accaggatga gctcgtgagc ccactcgatg tagttgccca
    17521 ggtagcggtt cagcaccagc gtgggaacca ggcaggcgag gtagacggcg ccggcccaga
    17581 ggagaggctg caggcccttg gcctcggttc ggcgatagag catcagggca ggcacagtgg
    17641 cgacgatcag caccaggtcg gagacactca gcggcccggt tcggatcatc accaaagtgg
    17701 ccggaaaggc cagtaccggc agcagcacca gatcagtcga ggcgaggccg agggccagga
    17761 tgatcacggc gacgaagatg gcgaggctgc cgtagtcggc gctcaggatc ccgagggcgc
    17821 ccgccaccaa taggacgagg gccacgccgg tcagcacccg tccccacttc acgaagcgaa
    17881 ggctgccaag ccccggtggt gcgaggcgac ggaagctaag ccccggtggc gagctgggca
    17941 ttggcgttcg ctcctgcgcg agccgcgacg agggcatcaa agagcaggga caggtggtcg
    18001 gagaggtcga tcactacttc gcgccagacg ccggcggaac ggcccatcgg atcgatgaca
    18061 tcgtgtcggg gataccgacc gaccaactcg ctgcgccggc gtaccgggcc aacggtggcc
    18121 atccagtcct gaacagtgga accgttcggt ggatgtcggc gaaggtattc ggtgtgttgc
    18181 ttcagcgtga acacgcgtgg ccataaccca gggtccagat gcaccacctc acgtgcatgc
    18241 tcgcgagtca tggtgaggat caggtccgca gattggagtt cagccagtcc cagctgccgg
    18301 ctgcgatgcc cggaaaggtc tagaccgaac tcggcggcaa cccgtacgcc gagagccggc
    18361 gagggccgtc cccccggcat caggcccatt gaggatgcca gcacggggaa cccgcgctcc
    18421 ctgccctcgc gcgctgcgag gagttcggcg atcggtgagc ggcacagatt cgcggtgcag
    18481 acgaagacca gtttgagtgc agcagggccg gcttcgaacg gcgcgggcga cagagagggt
    18541 gggtgcactc caagaccccc gatctgccgt gccgcaaccc gatcatgtca tcatagacgc
    18601 gtcccgaagc gaccgacgaa gtatccgtgg tcaccgggac gccgtcgcca ttcatcgaag
    18661 cctgaagcca cggtggacga tctggcatgg tgaccggagg gggaaggtac gtgaacgacc
    18721 ttgtggtctg ctcgctggag ccatgggacc atgtctggcg gcgaaaccag tttctcgtcg
    18781 acgggctgct tcgtcacgat cacacgctaa gggtgctctt cgtcgagccg gccagagatc
    18841 cgctccatct gatgcacatc gggcagaagc ccgggctggg acgcggtcgg ctcggtgtgc
    18901 cagggacgga cggacgcctg caccggctgg aactcaccaa gtggctcccg cgggccgccg
    18961 gcccaatggc cgacctgttg ctgcagcggg gagtgcgcga agctgcggcg gctatcggga
    19021 tgcggaagcc gctgctctgg gtgaacgacc ccggatgggc tggactggtt gccgcgacca
    19081 actggcccgc gatctatgac atcaccgacg actgggtggc cgcagaccgc ccccagcggg
    19141 agcacgaccg catcgtggcc aatgaggcga tcctgatgca gggttgccgg gaggtcgtgg
    19201 tgtgctcgga gcaactgaag cgaaccaaga gcgccatgcg cccggtcgaa ctcgtgccca
    19261 acgcggtcaa cctcgcctgg taccgcgatc cgcagccgcg cccggaagac ctgccccgag
    19321 gtcagatcgc cctgtacgcc ggcaccctgc acgaagaccg gctcgacgtc gacctcgccg
    19381 tggtactggg ccaggcactg agccgtgtcg gcgcgcaact ggtcttcgtc ggcccaaacg
    19441 cgctcggcag ccagaacacc gaccgactgg cggccatctc cggtgtacac ctactcggct
    19501 cgcggcccta tcgcgacctg ccggcgtacc tacagcatgc cgacgtgctc gtcgtgccgc
    19561 atctggtcaa cgacttcacc gacagcctcg acccgatcaa gttgtacgag tatcaggccg
    19621 cccagaggct gatcgtctcg acgccggtgg ccggcttcag gcagctggcg ggcctgcctg
    19681 gggtggcgat ctgcgaagcg cgggacttcc cggccggcgt ggttgaccgc ctgcagcggc
    19741 cgccggaatc gatcgggccc ttcgacgttg ccgactggga cgatcgcgtg gtcgccatgg
    19801 agcgcgtcct tagccgggct cgcgctccgc gcgacaacgg ctaggttccc ggccgagccg
    19861 caggtttagc tcgccctgga atgcgaccgg gcgcagggct cccctgggcg gcgtcgggat
    19921 tctggtccgg ttcgggcgag cgcgggctgg ccggcggagt ggccggcgac gcgtcggcgg
    19981 actcgggctg ctgggaaggc ttccacgtcg gtggagtcgg cgcttccaag cgagccacgt
    20041 cgaggggatc ctggtcgaag tcgtcctcgg ggtgcgtagt cccgtagtag tgggtgtagg
    20101 cgcgggtggc acgcttgtgc gaacggttca ccaccgcacc ggcgatctcg acgccattgc
    20161 cggccaggat ccggacggtc tcggccagcc ggcggtgcgt cgtggacttg gccgcggcca
    20221 caacgaccgt gccatcgacc tgagcgccga tcaccgccgc gtcagcgagt gcaagccctg
    20281 gcggggtgtc gaagatcacc acgtccgcga actggcgtag ttgccgtacg accagcttga
    20341 aggcgtcggc cgccagcaga tcagccggct cgcgccgcgc gatcccggcc gacaacaccc
    20401 gcagccgttc gtaggcagtg ggttgcagga gtttctccag actcgccgcg tcaaggccgt
    20461 ccacgattgc gtccgcgatg ccccgtgtgg gcgcgagccc gaagtaatgg tcgagcgtgg
    20521 ggcgacgcag gtcggcgccg accacgatca ccgatcgtcc gccacgggcc atcgagaccg
    20581 cgaggttcgc agtgataaag ctcttgccgt cgcccggctc agcgctggtg atggcgattg
    20641 cgctgtgcgg ctcggggtag aggacgttca gggacgttcg gagcgcgcga atgccctcat
    20701 ggaagggtgc tgaccgagta gtggtcgtgg gcagggtgcg gtcgtaccgg tgccgtcgcg
    20761 ggattcgcgt caactcggcc agcaccggga tgccgaggtc gtcgtcagcc gctaccgcgc
    20821 cacggagacg gtcgtcgaag aggtcccgga tgagggcgat tcccgcgccg gcgagaagcc
    20881 cgctggcgag gccgattagg agcaccgtca gagcgtccgt gccggttcga tcacccggca
    20941 gtgcggactg aaggattgag gccggaggtc cgctgttggc aatggacacc agaccgtcct
    21001 cgatgatgga cacatttccc agcgccgttt ccaggcgctg tctggcaacc ggatcgttcg
    21061 gcttcttggt ggcttgtcga accgcctcgg tgcgctcggc tttcgcctcc gtcaactcgg
    21121 tctgaagtcg ttcctgggtc gccttgacct gcgcggcgag gtggtcgacg tagacccgag
    21181 cgacaacttg agcgcgcagt tgagcctgct cggcgctgga agctatggcc acgatttcca
    21241 gtcggttgct gcgaacacct tccaccaggg tggtatcgat ggcggcctgc agcgcagacg
    21301 gctcatcctt ggtgagtggg ctcgcgatct cgatcagggc cggtgaagtg aggaaatccg
    21361 gatcgaggtc taggtcggtg tcggcgtagc cactccctcc gtcgaacatg cgggtggcag
    21421 tgagtgaata tcgcaccacc gaactcgatt tgtagacttc cggagtcaac tgaaggtagg
    21481 ccgccgcact tcccagcaca acaagcgttg tgagcgcgac caggagccac cttcgctgga
    21541 tgatctccag cacttgtcgc aaggtcattg aagcggactt ccctctcgcc gtgaggacca
    21601 agagcctctg ctattcgtgc gtccagggtg agatgttacc gggtgtgccg attcgcctct
    21661 tctagactag gaacccgggt gctacgtagg actgcccggt acccctcagc ccgcgaaacc
    21721 caggacaggc agcccgacca caccgggctc gaccgcgaac accgagcctg ccgccggctc
    21781 agcgccggcg tccaggttct cccgggaagt ggtgaggtag agcgtgtcca gttccggtcc
    21841 gccgaaacag caggcggtca ccttcgatac cccgggcacc tcgatgaccg cctccagagt
    21901 cccggacggc gagtagcggt tgacctggcc gccgccgtag cgcgccgtcc agaccccgcc
    21961 ctcggcgtcc acgcacagcc cgtccggtag gccgtcgtcg gcagcctcgg cgaagacccg
    22021 gcgtccgctg acgccctcga tcgggtcgta gtcgaaggcc caggtgaggc cggtgccgga
    22081 gtcgttcagg tacatcgtgg tggcgtccgg gctccagccg atgccgttgc tgatgctcaa
    22141 tccgtcgagc acccggtggg ggcggcgcga acccgcgtcc agctggtaca ggttcccggc
    22201 gccggcctcg aacgagtagg cgagagtgcc gatccagaac gccccgtccg ggtcgcagcc
    22261 gccgtcattg caccgcatcc ggtcgctttc gggcagctgg acgaaggccg ccaggtcgct
    22321 caggtcgtcg aagttgctga gcgcgaggtc gcgctcgcgg gcgacgatcg ccccgccacc
    22381 cattcgcgga cgcagcactg cggc
//
LOCUS       DAOPRQ010000043        22013 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_1983_length_22013_cov_11.368112, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000043 DAOPRQ010000000
VERSION     DAOPRQ010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 22013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22013
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1983_length_22013_cov_11.368112"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="PKJ61_07075"
     CDS             <1..420
                     /locus_tag="PKJ61_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016668607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOC13624.1"
                     /translation="SIWDYQAMAESDSMLNTPPTFGIYLLGKILAWVKASGGLTAMGE
                     RNRAKADALYGYIDASAYYSNPVALDSRSWMNVPFLQANADLDKTFVAEANAAGLTNL
                     AGHRSVGGMRASIYNAMPIEGVQALIDFMADFERRNG"
     gene            428..1597
                     /locus_tag="PKJ61_07080"
     CDS             428..1597
                     /locus_tag="PKJ61_07080"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HOC13625.1"
                     /translation="MTFKIKTLNAISHTGLSRLPKEKFETSSDVESPDALILRSANLH
                     GVAIPESVLAVARAGAGTNNIPIADYSAVGIPVFNTPGANANAVKELVVAALFLAARN
                     IVPAAAFAHRLDGDAATMDKAVESGKKNFVGFELPGRTLGVIGLGAIGVEVANAASAL
                     GMRVLGYDPGITVEHAWNLSSSVEHVTNLDQLLKRADIITLHVPLIDATRGLIGAEQL
                     TKLHKSSVVINFARGPIVDEDAVVEALESGRLRAYICDFPSPALNKRDRVVTLPHLGA
                     STAEAEENCAVMAANQLRDYLLDGTIINSVNFPNAVLPRTVGTTRIAIANSNVPNMVG
                     QISTLLAETGLNIADLLNKSRGELAYTLVDVDGEVPADVVAAVRAIPGVLSARVL"
     gene            complement(1656..3923)
                     /locus_tag="PKJ61_07085"
     CDS             complement(1656..3923)
                     /locus_tag="PKJ61_07085"
                     /inference="COORDINATES: protein motif:HMM:NF025229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOC13626.1"
                     /translation="MRRSFAVMLTTALLATGGILLPPAAAAEDGLTPDIHVPLPSMWA
                     SQSLTAATGKSLVRTVYVNSHQQDEEYSIDNGATWRPLPAELAGSGSLAGYGGEFIKL
                     RRTVISPGGEGCETSKLNGFTVWNPNTGVQRKVELSAADASVEVECLWLTDAVRGLVL
                     TSDGRVFDISGATAVQRKIKPGPGVPDGALPVGISADGATVALRASVYDPKGVRSERT
                     YLAVAPISGAAGSAPIEIAGLTDVVVSGQNVHYLIRTAAKLTVCRAPIATPAKAKCVS
                     LRKGDQDYEAQLEVSQGLEQVTWRSTLWLVSGAQVKALKTSGKVTELRGTGFRDPVRP
                     LVRGLDPQRGDVYGQVSPKLGVSWSIPSRKVPTRISALALSTNRVTAVDSRPHAAEAG
                     PAPLWYRDLQGTSVGPERRLPGSAELTEELFTSAGRTVLDCCLSVKARFYDQGRLILK
                     QKLPFNVDEISGPYIDGGDRVRRMDGATFMTGADGDSAVFGSLVAWLDRTDTVTIRDL
                     AAPGTADIVVHLPKQAYEYNFGRLQLWGDWVGISWGDANQGQPESSLVTNFRTGETHT
                     TVGELQDLGDGYALLHRFGALAEGEYELPYTVVTWSFGSDEIRAVPVPQGNQVAIDGI
                     GRVAWAADTAIEIAKVPGAGTSAPRLLGVLAKASVKAGKKWTPQVDMSKALKAGVLEI
                     RDRSGRVVRTISTKATATGSLRTLSWDGRNSAKKKVPAGTYTWTLRADATDGTGTLTD
                     VTGTAAASGEVKVIR"
     gene            complement(4041..4445)
                     /locus_tag="PKJ61_07090"
     CDS             complement(4041..4445)
                     /locus_tag="PKJ61_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014630045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HOC13627.1"
                     /translation="MGVTYLLDTHVLLWLWGDPGRIAPGVRKALGDPKNHLLVSAASA
                     MEVSTKTRRGKLELGHALASTWAARVAEIAAEDLQITSAHALLAGSMRWSHRDPFDRL
                     LVAQALTEGVPLVSVDPAMVGVPALQVIDPRS"
     gene            complement(4436..4657)
                     /locus_tag="PKJ61_07095"
     CDS             complement(4436..4657)
                     /locus_tag="PKJ61_07095"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HOC13628.1"
                     /translation="MTTVNVQEAKTRLSELLARVERGEEIVVARAGTPVARLVPVDLA
                     WTRPFGRLAFEVPEDFDAPLDEDELVAWE"
     gene            complement(4725..6482)
                     /locus_tag="PKJ61_07100"
     CDS             complement(4725..6482)
                     /locus_tag="PKJ61_07100"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016455011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOC13629.1"
                     /translation="MPTNALDHLSPAFPGRAAWGTAARLRAWQVAALDRYRELGQQDF
                     LAVATPGAGKTTFALRVATGLLADRVIEQVIVVTPTEHLKVQWADAAAKVGLHLDPGL
                     GGSKRGRTRQYQGQAVTYAGVAAATHAYESRTVNAKTLVIFDEVHHAGDALSWGEASA
                     QAFALATRRLALTGTPFRTDANPIPFVRYVEEADGTRKSVADYTYGYAEALRDHVVRP
                     VVFLAYGGGMRWRTRAGDELAASLGEALPKDVTAQAWRTALDPAGEWMPAVLASADRR
                     LTEVRRHVPDAGGLVIASDQRQARAYARALAQITSQQPTLVLSDDSGASRKIDEFSES
                     EDRWLVAVRMVSEGVDVPRLAVGVYATATSTPLFFAQAVGRFVRSRRRGELASVFLPT
                     VPILLAHAGALERQRDHVLGKPRSTEGDLWSPEEALLAEANRTRRTPEFEGQQETLEA
                     NAIFDHVLFDARAYGMFAEPSSQDEADYLGLPGLLESDQVATLLADRQRRQLARSARR
                     DRREAVPPEVSLHRALESRRKELNSLVSQVARVRGVPHSHIHAGLRREAGGPPLAKAT
                     TEQVEARISLARDWLAKSA"
     gene            6699..7544
                     /locus_tag="PKJ61_07105"
     CDS             6699..7544
                     /locus_tag="PKJ61_07105"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOC13630.1"
                     /translation="MSSLFSHHAEPAQPWFRIGRLEVTTTMWVVLAVVASWVAWMLAP
                     SLPGALDYSTYAVLSGDIWRLITWPFANTLSSIWSVLTLFFFWYFGTDLERQVGRTRM
                     VWLLVGIWGSLTLAATLVGFVLGGQAFGGISLIQFTVLLLWIAEYPNRPFFFGIPAWM
                     IGAVLLGIQVVGMIAGRDGAGLLSLLLSLAFVAIAARRIGLLSDYPWIPGRAAPRTAS
                     VRSARTTRGRTQTRTPSRDERRQQTDRERLDALLDQINESGIHSLSDAQRKEMKRLSE
                     RLRGH"
     gene            complement(7530..7967)
                     /locus_tag="PKJ61_07110"
     CDS             complement(7530..7967)
                     /locus_tag="PKJ61_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HOC13631.1"
                     /translation="MAELQPEASRPRLDDSPGGSVTDVPRPSPEERTILWWLTAVVLV
                     VHVVGIYLPGSPDGSLELPGVPGVDKLIHASLFGLPLYLLAVLTRRIWLWAGFFAAMA
                     GISEVIQWRFIPYRDGDVWDAVADLVGIAIAVLLLLRRRSVTA"
     gene            complement(7960..9441)
                     /gene="gatB"
                     /locus_tag="PKJ61_07115"
     CDS             complement(7960..9441)
                     /gene="gatB"
                     /locus_tag="PKJ61_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020574762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HOC13632.1"
                     /translation="MSLMEFDDALVAFDPVLGLEVHVELNTASKMFCGCANAFGAEPN
                     TQTCPVCLGLPGSLPVVNARAIESAIRIGLALNCDIAQWCRFARKNYFYPDMTKDFQT
                     SQYDEPIAFDGFVDLEVDGEVFHIEIERAHMEEDAGKLVHTGFTGRIGDADYSLVDYN
                     RAGTPLIEIVTRPIRGTGAKAPQVAKAYVSYLRDLMLALGVSDVRMEQGSLRCDANVS
                     LMPKGETELGLRTETKNVNSLRSIERALNYEIRRQAALLAEGGRVHQETRHWHEEGYT
                     TPGRSKEQAEDYRYFPEPDLVPVAPSREWVEQLRGTLPEVPAVRRARLQETWGFTDLE
                     MQDAVNGGALDVIEATVAAGATPQSARKWWLTELARRANEAGVELAELAITPSQVAAV
                     QALVDAGTVNDKLARQVIEGVLAGEGTPAEVVGARGLAVVSDDGALTAAVERAIEANP
                     DVAERIREGKLAAAGALIGAVMRDMRGQADAARVRELILARLG"
     gene            complement(9438..10967)
                     /gene="gatA"
                     /locus_tag="PKJ61_07120"
     CDS             complement(9438..10967)
                     /gene="gatA"
                     /locus_tag="PKJ61_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020574761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HOC13633.1"
                     /translation="MSALLRAGATELAGLIASGEVSAREVTAAHLDQIAAVDDRVKAF
                     LHVDGERALASADAVDAKRAAGEKLGPLAGVPLAMKDIFTMQGAPTTCGSRILEGWMP
                     PYNSTITDKLLAADVIVLGKTNMDEFAMGSSTENSGYFATHNPWDNALIPGGSGGGSA
                     AALAAFEAPLAIGTDTGGSIRQPASVTGTVGVKPTYGGTSRYGVVAMASSLDTPGPCA
                     RNVRDAALLHSVIGGWDPRDSTSINAPLPDLLGAVERADVAGMRIGVVTEFGGEGYQP
                     GVEQRFSEALAVLEQLGAQIVEVSCPNFEYALAAYYLIMPSEVSSNLARFDAMRYGLR
                     VGDDGVNSVEEVMNLTRGAGFGAEAKRRIIIGTYALSSGYYDAYYGSAQKVRTLIKRD
                     FDAAFDKVDVLVSPTTPTTAFPIGERVDDPLAMYKADLCTIPSNLAGNCALSLPVGLS
                     PDNGLPVGFQVMAPVLADDRLYRIGAALEAELNRNWGASLLDQFADSPAGAYLNVGSI
                     R"
     gene            complement(10964..11260)
                     /gene="gatC"
                     /locus_tag="PKJ61_07125"
     CDS             complement(10964..11260)
                     /gene="gatC"
                     /locus_tag="PKJ61_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016455923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HOC13634.1"
                     /translation="MALTTGDVGRLGELARIRLTPDELAHLAPQLDVILQSVATVTEV
                     ATADVPPTSHALPLTNVFRDDVVTPSLPVAAVLAAAPATEDNRFRVPRILGEEA"
     gene            11360..11980
                     /locus_tag="PKJ61_07130"
     CDS             11360..11980
                     /locus_tag="PKJ61_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007433840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HOC13635.1"
                     /translation="MFLLRVVLPDRPGSLGAVATAMGLVHADINAVEIVEKYEGFAID
                     DFMVELPATVAADSLITACSTVPGVEVVWLSHYPESWGLQADVDVLNAMTEDPEHAEE
                     ILTLEAPAVFRVSWAVLVDRRTGEVLIQTELAPDLTPADVAGFGALDQLHDLELPEGW
                     LSGWGDTIIAVAPFRTDATIVLARRGGPEFLKSELARLNHLAALAL"
     gene            12333..12554
                     /locus_tag="PKJ61_07135"
     CDS             12333..12554
                     /locus_tag="PKJ61_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOC13636.1"
                     /translation="MAVGPEAGLGALAELAASGALAGHHLLPAAQADLCRRCGRVREA
                     ERHYREAIELAPTDPERRFRQRRLAALGT"
     gene            complement(12682..12885)
                     /locus_tag="PKJ61_07140"
     CDS             complement(12682..12885)
                     /locus_tag="PKJ61_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13637.1"
                     /translation="MPEGLNSDLNLNVGAPPRVRPGMGEWGPQEVPTTRQKRRMRWWT
                     LLALLGVLAAVVGYGYWMYTILR"
     gene            complement(12895..14964)
                     /gene="ligA"
                     /locus_tag="PKJ61_07145"
     CDS             complement(12895..14964)
                     /gene="ligA"
                     /locus_tag="PKJ61_07145"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002521420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOC13638.1"
                     /translation="MTEDGTRARHAELSELITDHRWRYYVLDAPTVADAEFDRLMAEL
                     TAIEEAHPELRTPDSPTQQVGGAPSATFAPVAHLLPLMSLDNVFSLEELDKWLTRAAT
                     LAGTDPATAGYLCELKIDGLALDLVYRGGRLVSAATRGDGTVGEDVTANVRTIASIPA
                     QLTGEAVPELVEVRGEVFMRPDDFARLNEGLVAAGKAPFANPRNSAAGSLRQKDPRVT
                     ASRKLDFLVHGIGEFSGPPLERQSQGYKLLHGWGLPVSAHYRICHRVAEVKEYVAHYG
                     EHRHSVEHDIDGVVIKVDDRELQARLGHTSRAPRWAIAYKYPPEEVTTKLLDIRVNVG
                     RTGRVTPYAVMEPVFVAGSTVEMATLHNASEVQRKGVLIGDTVVLRKAGDVIPEVLGP
                     VAELRDGTERAFVMPTRCPACGTDLRPEKEGDADIRCPNQRSCPAQLRERLFHLASRQ
                     ALDIEVLGWQSADALLSAGLLTDEGDLFDLTAAKLVEAGVFTTKAGELSANGRKLLAN
                     LDDAKSRPFARFLVALSIRHVGKGVAPDVAAAFPSIDALASASVEELSAVSGIGPTLA
                     ASIAEWFTVDWHNAIVAKWRAAGCRLADEPRAEAPGEPQTLAGLTIVVTGAVPGYSRD
                     GATAAIEARGGKAAGSVSSRTSYVVAGESAGSKLDKAIALGVPVIEADAFERLLTAGP
                     EGLITAD"
     gene            complement(15079..16110)
                     /locus_tag="PKJ61_07150"
     CDS             complement(15079..16110)
                     /locus_tag="PKJ61_07150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HOC13639.1"
                     /translation="MVAFTGLGSLPGTDFGAALRMTFDKVPELPYLPELPARGPWAAM
                     IGRGLGLPSGLPASLEAGEWRLTDAPGVDQRRARATWRDDLDRLEEIGDGYTGWFKVA
                     VAGPWTLSAALAVAHTGRVLADLGARRDLAGALADGIGELLVDLGKRLPGARLILQVD
                     EPALPAVAAGAVPTPGGYFRHRAVDLPEVCRSLGLLTAEPARRGLAVRTVLHSCAPWQ
                     RPGGWPLRSLLHGDSTPAGFDGISLDLDQLKTADWDALAAAADAGAELYLGCLPTSAS
                     PRPLGVDATRVRALRALERIGADSGISGRLVLTPACGLAGWTSAEVSRGFEVLNVAST
                     QLAEQLSGG"
     gene            complement(16110..17240)
                     /gene="mnmA"
                     /locus_tag="PKJ61_07155"
     CDS             complement(16110..17240)
                     /gene="mnmA"
                     /locus_tag="PKJ61_07155"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOC13640.1"
                     /translation="MRVLAAMSGGVDSAVAAARLVDAGHEVVGVHLALAKNSEAYRSG
                     ARGCCSLEDSHDARRAADALGIPFYVWDFAERFAADVVADFVDEYAAGRTPNPCLRCN
                     EKIKFAAVLERGRALGFDAVATGHYARIERSGDTVSLYRSPEPAKDQSYVLGVLDADQ
                     LRHSLFPLGADTAKAEVRAEAAARGLLVARKPDSHDICFIPTGDTAGFLRSRLGSQVG
                     DIVDETGDVVGAHSGYQQFTVGQRHGLHLSVPAADGRPRYVLRIDPVANTVQVGPRER
                     LAVQTIEAIRPTWTEGFVAGAWRGLVQVRAHGEAMPATIHVVTIPEELSEERGKRHEG
                     PPPACVRIVLDQPALGIAPGQAAVYYDGDRVVGSATISATAA"
     gene            complement(17311..18435)
                     /locus_tag="PKJ61_07160"
     CDS             complement(17311..18435)
                     /locus_tag="PKJ61_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HOC13641.1"
                     /translation="MTSRSYLDHAAGSPALPEVVAAVAATLPLAGNPSSLHASGRAAR
                     RLLEESRESLADHLGALPTEVVFTSGGTEANNLALLGGATDGRPVVALSAIEHPSVAA
                     ARTRLETVTLPVAASGHVEPAVVAELLTDRVGLTSVMWVNNETGAIQPVAEVADAAHA
                     AGSLAHSDAVQAFGHLPIDFAASNLDLLTVSGHKLGAPVGIGALVVRRGVKLRPTGFG
                     GGQEARLRSGSVPVALAVGFATAARVAIERQAAEAERLGELRNLLISGALAGVAGCRA
                     NTVAPCSPAIVNLTFEDVRADDVLLLLDAAGIDASTGSACTAGVHQPSEVLLAMGRGV
                     ADASASLRFSFGPTTTRAEIEALVKLLPGVVARARAASAT"
     gene            18550..20772
                     /gene="glgX"
                     /locus_tag="PKJ61_07165"
     CDS             18550..20772
                     /gene="glgX"
                     /locus_tag="PKJ61_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013160973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HOC13642.1"
                     /translation="MTQSEQTHPSGDTSLGAQVTAEGTLFSLWAPRATRVELALIDEH
                     RRQTNHDLVADDAGVWEVFVPAVGAGQRYGYRVHGKWSPETGARFNPAKLLLDPYARA
                     ITGGVDYSGPITDHTPGSNFLPDPTDSFAAVPLSVVVADSDAPRPLSRPLRESELVIY
                     EAHLKGYTQLHPAVPEHLRGTYAGMAYPAVIDHLLKLGVNAVEFLPLHHFVSEPFVVG
                     RGLVNYWGYNTLGFFAPHAAYSSSGTLGEQVSEFKEMVSALHDSGIAVILDVVYNHTG
                     EGGHDGPTLSFRGIDHAAYYRLTDDKRNDYDVTGCGNAVDTSKPGVLKLVLDSLRYWV
                     TEMGVDGFRFDLQTTLIRDHAHHVDQNHEFKRLVAADPVLAGKVMISEPWDLGPYGYQ
                     VGRWGKGWSEWNDRFRSFARDFWRGAIPGVQELATRLCGSSDIYDHGGRPATSSVNFV
                     TAHDGFTMRDLVTYDLKHNEANAERNRDGADDNRSWNHGYEGETDDPTIIASRQRTAR
                     NMMATLLLSAGIPMITAGDEMGRTQIGNNNAYCQDSPVSWVNWYSQDDWSEQEELTRA
                     LLQLRADYPLLRPEGWRSHGEVTDGSGHGLGRSQIAWFAESGTEMTTEQWHDPGRRTL
                     GEYLSDGSQALLIYFHSGWEPVELSLPGAPWALAYRILAHTGTSGELPRKRLSPGSTV
                     TLPARTVVVFGAEVLTGVRPVPVSPVTPDEAEADVADAPDTVGQDLPPNGIPNARLGL
                     "
     gene            20898..>22013
                     /locus_tag="PKJ61_07170"
     CDS             20898..>22013
                     /locus_tag="PKJ61_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015070614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3416 domain-containing protein"
                     /protein_id="HOC13643.1"
                     /translation="MTVPRGFGRIPVTDVYPVIEGGAYPAKAVVGELIPIRATVFREG
                     HDAVNASVILTAPDGREQRVDMTPMEPRGLDPWEAWVRPDAEGAWTFRVEGWSDPWAT
                     WLHNAEAKLPVGVDIELVCLEGRDLLDRTAASAKAANDPVQASILHATAMLLIPERPA
                     TDLIEVATAKGISRAMEQYGPRDLLSATADFPLFVDRKRALFSSWYEFFPRSQGANFD
                     PATKKWTSGTFDGSHQRLEEVAAMGFDVVYLPPIHPIGRAFRKGRNNTLTPGPNDPGS
                     PWAIGAAEGGHDAIHPDLGDWEAFDRFVAKATECGLEIALDFALQASPDHPWVKAHPE
                     WFTTRIDGTIAYAENPPKKYQDIYPINFDNDPEGIYHE"
BASE COUNT         3529 a         7855 c         7225 g         3404 t
ORIGIN      
        1 tcgatctggg actaccaggc gatggccgag tcggactcga tgctgaacac cccgccgacc
       61 ttcggcatct acctgctggg caagatcctg gcctgggtga aggcgtccgg cgggctgacc
      121 gcgatgggtg agcgcaaccg agcgaaagcg gacgctctct acggctacat cgatgcctct
      181 gcctactact cgaacccggt cgcgctggac tcccggtcgt ggatgaacgt gccgttcctg
      241 caggccaacg ccgacctcga caagaccttt gtggccgagg cgaatgccgc cgggctgacc
      301 aacctcgccg gccaccgctc ggtcggcggc atgcgggcca gcatctacaa cgcgatgccc
      361 atcgagggcg tccaggcgct gatcgatttc atggccgact tcgaacgccg gaatgggtga
      421 cgcgccgatg accttcaaga tcaagacgct caacgcgatc agccacaccg ggctctcccg
      481 gctgccgaag gagaagttcg agaccagctc ggacgtcgag tccccggatg ccctgatcct
      541 gcgctcggcc aatctgcacg gcgtggccat ccccgaatcg gtgctggctg tggcccgggc
      601 gggtgcaggc acgaacaaca tcccgatcgc cgactactcc gcggtgggta ttcccgtctt
      661 caacaccccc ggcgccaacg cgaacgcggt caaggaactg gtggtggccg cgctgttcct
      721 ggccgcacgc aacatcgtcc cggccgcagc gttcgcgcac cggctggacg gcgacgcggc
      781 gaccatggac aaggccgttg agtccgggaa gaagaacttc gtcggcttcg aactgcccgg
      841 acgtaccctc ggcgtcatcg gactcggcgc gatcggcgtc gaggtggcca acgcggccag
      901 cgccctgggc atgcgggtgc tcggctacga cccgggcatc acggtcgagc acgcctggaa
      961 cctgagctcg tcggtggagc acgtgacgaa cctcgaccaa ctgctcaagc gggccgacat
     1021 catcacgctg catgtgccgc tcatcgacgc gacccgcggc ctgatcggcg ccgagcagct
     1081 gaccaagctg cacaagtcgt cggtggtgat caacttcgct cgcggcccga tcgtcgatga
     1141 ggacgccgtg gtggaggctc tggagagcgg ccggctgcgt gcctacatct gcgacttccc
     1201 atcgcccgcg ctgaacaagc gcgaccgggt ggtcacattg ccccatctgg gtgcgtccac
     1261 cgcagaggca gaggagaact gcgcggtgat ggcggccaac cagcttcggg actacctgct
     1321 ggacggcacc atcatcaact cagtcaattt cccgaacgcc gtgctgccac ggaccgttgg
     1381 caccacccgg atcgcgatcg ccaactcgaa cgtgccgaac atggtcggcc aaatctcgac
     1441 gctgctcgca gaaacaggcc tgaacatcgc cgacctgctg aacaagtcgc gcggcgagtt
     1501 ggcctacacc ctggttgacg tggacggcga agtgccggcc gacgtggtcg ccgcggtgcg
     1561 ggcgatccca ggggtgcttt cggcgcgcgt gctgtagccc aaccaactga gccggacgaa
     1621 gtcctgatca gggcctcgtc cagctcagcg agtactcagc ggatgacctt gacctcgccg
     1681 gacgcggctg cggttccggt cacgtcggtg agggtgccgg tgccgtcggt tgcgtccgcc
     1741 ctgagcgtcc aggtgtaggt gccggccggc accttcttct tcgccgagtt ccggccgtcc
     1801 cagctcaacg tgcgtagtga gccggtcgcg gtcgccttgg tgctgatcgt acgcaccacg
     1861 cgtccgctgc ggtcgcggat ctccagaacg cccgccttca acgccttgct catgtcgacc
     1921 tgtggcgtcc acttctttcc agccttcacc gaggccttcg ccagcacgcc aagcagccgc
     1981 ggtgcgctgg tgcccgcacc cgggaccttc gcgatctcga tcgcggtgtc cgcggcccaa
     2041 gccacgcgtc cgatgccgtc gatggccacc tggttgccct gcggaaccgg aaccgcgcgg
     2101 atctcgtcgg acccgaagct ccaggtgacg accgtgtagg gcagctcgta ctcgccctcg
     2161 gccagggcgc cgaagcggtg gagcagcgcg tagccgtccc cgagatcctg cagctcacca
     2221 acggtcgtgt gggtctcgcc ggttcggaag ttcgtcacca ggctgctctc gggctggccc
     2281 tggttcgcat cgccccagga gatcccgacc cagtcgcccc agagctgcag ccggccgaag
     2341 ttgtactcgt aagcctgctt cggcaggtgc accacgatgt cggcggtacc gggcgcagcc
     2401 aggtcgcgga tcgtgacggt gtccgtccgg tcgagccagg ccaccaggct gccgaacacc
     2461 gccgagtcgc catccgcccc tgtcatgaag gtggctccgt ccatccggcg cacccgatct
     2521 ccgccgtcaa tgtagggacc gctgatctcg tccacgttga agggcagctt ctgcttcagg
     2581 atcaatcggc cctggtcgta gaaccgggcc ttcaccgaga gacagcaatc gagcaccgtg
     2641 cggccggctg aggtgaacag ctcttcggtc agctcggcgg agccgggcag ccggcgctcc
     2701 gggccgacgc tcgtgccctg caggtcgcga taccaaagcg gggctggccc ggcctccgcg
     2761 gcgtgcggac ggctatcgac ggcggtgacg cgattggtgc tgagggcgag cgcgctgatc
     2821 cgggtaggca ccttgcggga cgggatcgac cacgacacgc cgagcttcgg gctgacctgg
     2881 ccgtagacat ctccccgctg cggatcgagc ccccggacga gtggacgcac cgggtcgcgg
     2941 aagccggttc cgcggagctc ggtcaccttg ccggacgtct tcagcgcttt cacctgggca
     3001 ccggacacca gccagagcgt cgaacgccag gtgacctgct cgagaccctg ggagacctcg
     3061 agttgggcct catagtcctg atcgcccttg cggagcgaga cgcacttggc tttcgccggc
     3121 gtcgcgatcg gggctcggca gaccgtgagt ttggcggccg tccggatcag gtagtgcaca
     3181 ttctggccgg aaaccacgac gtcggtgagc ccggcgatct cgatcggcgc cgagccggcg
     3241 gccccgctga ttggggcgac ggcaagatac gtccgctcac tccgaacacc cttggggtcg
     3301 tagacggaag cgcgtagcgc gaccgtggcg ccgtccgccg agatgcccac tggcaacgcc
     3361 ccatccggca cacctggtcc gggcttgatc ttgcgctgta ccgcggtggc gccgctgatg
     3421 tcgaagaccc gtccgtccga ggtcaacacc agcccgcgca ccgcatcggt gagccacagg
     3481 cactccacct cgacggacgc gtccgctgcc gacagctcga ccttccgctg gacgcccgtg
     3541 ttggggttcc acacggtgaa tccgttcagc ttcgaggttt cgcagccctc tccgcccgga
     3601 ctgatcaccg tccgacgtag cttgatgaac tcgccgccgt agcccgccag cgagcccgag
     3661 ccggccaact cggcgggcag cggccgccaa gtcgccccgt tgtcgatcga atactcctcg
     3721 tcctgctggt gcgagttgac gtacaccgtg cgcaccaggg acttgccggt cgccgcggtc
     3781 aggctctggg atgcccacat cgacggcagc ggcacgtgga tgtcgggggt caacccgtcc
     3841 tccgccgccg cggccggggg caacaggatg cctcctgttg ccaatagtgc ggtcgtgagc
     3901 attacggcga acgagcgacg catagcgact ccatcaggtc agcaagtaat tcacgctatc
     3961 cggagttccg ctgcgtccag ttagaaaggc atgagatttc gatgagacct gggcactcac
     4021 ctggctaact gctgcccgga tcacgaccgc ggatcgatga cctgcaacgc cggcacgcca
     4081 accatggcgg ggtccaccga caccagcggc acaccctcag tgagggcctg agccaccagc
     4141 aggcgatcga aggggtcacg atgcgaccac cgcatcgagc cagccagcag agcgtgcgcg
     4201 gaggtgatct ggagatcctc ggcagcaatc tcggccactc gggccgccca ggtcgacgcc
     4261 agcgcgtgac cgagttccaa tttgccgcgg cgagtcttgg tcgagacctc catcgcgctg
     4321 gccgccgaga caagcaggtg gttcttcggg tctccgagcg ctttgcgcac acctggggcg
     4381 atgcggcccg gatcacccca aagccagagc agcacatgcg tgtcgagcag gtaggtcact
     4441 cccatgcgac caactcatcc tcgtcaagcg gagcatcgaa gtcctctggc acctcgaatg
     4501 ccaaccttcc gaacggcctg gtccaggcca ggtcgactgg caccagccgc gcgaccgggg
     4561 taccggcgcg ggcaaccacg atctcttcac cgcgctcgac gcgggccagg agctccgaca
     4621 ggcgagtctt cgcttcctgc acattcaccg tcgtcatggc gacagcctag gttgaccaga
     4681 ccagcttggt caatggcccg gcgtagccgt cgtgggccga gcactcaggc ggactttgcc
     4741 agccaatcac gagccaggct gatccgcgcc tccacctgct cggtggtggc cttcgccagc
     4801 ggcggccccc cggcctcccg gcgcagtccg gcatggatgt ggctgtgcgg cactccacgg
     4861 acgcgcgcca cctgggagac caggctgttc aactccttcc ggcggctttc aagggcgcgg
     4921 tgcaggctca cttcgggcgg caccgcttcg cgtcggtcgc ggcgggccga cctggccaac
     4981 tgccgacgct gtcgatcggc gagcaacgtc gccacctgat cggactccag caacccgggc
     5041 agccccaggt agtcggcctc gtcctggctg gacggctcgg cgaacattcc gtaggcgcgg
     5101 gcgtcgaaga gcacgtgatc gaagatcgcg ttggcttcca gggtctcctg ctggccctcg
     5161 aactccggcg tccgccgggt gcggttcgcc tcggccagta gtgcctcctc gggagaccac
     5221 aggtcaccct cggtgctgcg gggcttgccg agcacgtggt cacgctggcg ttccagcgcg
     5281 ccggcgtgtg ccagcaggat cggcaccgtg ggcaggaaga cactcgccaa ctcaccgcgc
     5341 cgacggctgc ggacgaagcg tccgaccgcc tgggcgaaga acagcggggt cgaagtggcc
     5401 gtggcgtaga cccccaccgc cagccgcggc acgtccaccc cctcggagac catgcggacg
     5461 gcgaccagcc agcggtcctc ggattcggag aactcgtcga tcttccggct cgctccggaa
     5521 tcgtcggaca gcaccagggt cggctgctgc gaagtgatct gtgccagggc ccgcgcatag
     5581 gcgcgggcct gtctttgatc gctggcgatc accagcccac cggcgtccgg gacgtgccgg
     5641 cgcacctcgg tcaaccgtct atcggcggac gccagcacgg ccggcatcca ctccccggcc
     5701 gggtccagcg ccgtccgcca ggcctgcgca gtcacgtcct tgggcagcgc ctcccccagg
     5761 gacgcggcca gttcgtcgcc ggcccgcgtc cgccagcgca tgccgccgcc gtaggcaagg
     5821 aagaccaccg gacgcaccac atggtcgcgt agcgcctcgg cgtagccata ggtgtagtcc
     5881 gccaccgact tgcgcgtgcc gtccgcctcc tcgacatagc ggacgaacgg gatcgggttg
     5941 gcgtcggtgc gaaacggtgt gccggtgagg gccaaccggc gggtggccaa cgcgaacgcc
     6001 tgggcggacg cctcgcccca gctgagcgcg tccccggcat ggtgcacctc gtcgaagatc
     6061 acgagcgtct tggcgttcac cgtccgcgac tcgtaggcgt gcgtggctgc cgccaccccg
     6121 gcataggtga ccgcctggcc ctggtactgc cgggtgcggc cacgcttgct accgccgagc
     6181 cccggatcca ggtgcaggcc gaccttcgcg gccgcgtctg cccattgcac cttcaggtgc
     6241 tcggtcgggg tgaccacgat cacctgctcg atcacccgat cagcgagcaa cccggtcgcc
     6301 acgcgcaaag cgaaggtcgt cttccccgca cccggcgtcg ccaccgcgag aaaatcctgc
     6361 tggccgagtt cacggtaacg atccagtgcg gccacctgcc acgcccgcaa ccgggccgcc
     6421 gtgccccagg cagcgcgacc agggaacgcc ggagagaggt ggtcgagggc gttggtcggc
     6481 acagctctca cctcctgggc gcatcgtcgg tctttccggc gagccggccc gggaccatcc
     6541 ggacaaccag gatcaacacc tatcggggcg gatcgaactc gccgccctag cttcgttggc
     6601 gctccgccga tgggcaagct gacacgccca gccacggagc gttcatcgaa gtcggccgac
     6661 gccgatgtcg gcttgggaag tctggcaacc ttggcggtat gagttctctg ttctcgcacc
     6721 acgcggaacc cgcgcagccg tggttccgca tcggtcggct ggaggtcacc acgaccatgt
     6781 gggtcgtgct ggcggtagtg gcgtcatggg ttgcctggat gctggcccca agcctccccg
     6841 gtgcgctcga ctactcgact tatgccgtgc tgagcggcga tatctggcgc ctgatcacct
     6901 ggccgttcgc caacacgctc tcctcgatct ggagcgtgct gacgctcttc ttcttctggt
     6961 acttcggcac cgatctcgaa cgccaggtcg gacgtactcg catggtctgg ctgctggtcg
     7021 ggatctgggg cagcctcacc cttgcggcga ccctggttgg cttcgttctc ggcggtcagg
     7081 cgttcggcgg catctcgctg atccagttca cggtcctgct gctgtggatc gctgagtatc
     7141 ccaatcggcc gttcttcttc ggcatcccgg cgtggatgat cggcgcggtg ctgctcggca
     7201 tccaggtcgt tgggatgatc gccggacgcg acggcgccgg gctgctcagc ctgctgctca
     7261 gcctcgcctt cgtggcgatc gctgcgcgac gcatcggcct actcagcgac tacccgtgga
     7321 ttccgggccg cgccgcgccc cgcaccgcgt ccgttcggtc ggcgcgcacg acccgtggtc
     7381 gcacccagac ccgaacccca agccgcgacg agcgtcggca gcagaccgac cgcgaacgcc
     7441 tggacgccct cctcgaccag atcaacgaga gcggcatcca cagcctgagc gatgcgcagc
     7501 gcaaggagat gaagcgactc agcgagcggc tacgcggtca ctgaacggcg gcgcagcagc
     7561 aacagcaccg caatcgcgat cccgaccagg tcggccaccg cgtcccaaac atcgccgtcg
     7621 cgatacggga tgaaccgcca ctggatcacc tcgctgatcc cggccatcgc ggcgaagaag
     7681 ccggcccaca gccagattcg acgcgtgagc acagccagca ggtacagcgg taggccgaac
     7741 aacgaagcgt ggatcagctt gtccacgcca ggcaccccgg gcagttcgag cgagccgtcg
     7801 ggcgagccgg gcagatagat cccgaccacg tgcaccacga ggaccaccgc ggtcagccac
     7861 caaaggatgg tccgctcctc cggcgacggt ctcggcacgt cggtgacact cccgcccggg
     7921 ctgtcatcca acctcggacg ggacgcctcg ggctgcagct cagccaagcc gggccaggat
     7981 caactcgcgg acgcgcgcag cgtcggcctg gccgcgcatg tccctcatca ccgctccgat
     8041 cagcgcaccg gcagcagcca gcttgccttc gcggatgcgc tcggcaacgt ccggattggc
     8101 ttcgatagcg cgctccacag ccgcggtgag cgcaccgtcg tcgctgacca cggccagccc
     8161 gcgcgcgccc acgacctcgg ccggtgtgcc ctccccggcc aacactccct cgatgacctg
     8221 acgcgcgagc ttgtcgttga ccgtcccagc gtccaccagc gcctgcaccg cggccacctg
     8281 ggacggggtg atggccaact cggcgagctc gacgccggcc tcgttggcgc ggcgagcgag
     8341 ctcggtcagc caccacttgc gcgcgctctg cggggtcgcc ccggcggcga ccgtggcctc
     8401 gatcacgtcc agcgcgccgc cgttgaccgc gtcctgcatt tccaggtcgg tgaagcccca
     8461 ggtctcttgc agccgggccc ggcgcaccgc cggaacctcc ggcagggttc cccgtagctg
     8521 ctccacccac tcccggctcg gcgccaccgg caccaggtct ggttcgggga agtagcggta
     8581 gtcctcagcc tgctccttgg accggcccgg ggtggtgtag ccctcctcgt gccagtgccg
     8641 ggtctcctga tggacgcgac cgccctcggc cagcagggcc gcctgccgcc ggatctcgta
     8701 gttcagcgcc cgctcgatgc tgcgcaacga gttcacgttc ttggtctcgg tgcgcaggcc
     8761 cagctcggtc tcgcccttgg gcatcaggga cacgttggcg tcgcagcgca gcgagccctg
     8821 ctccatccgg acgtcggaga cgcccaacgc cagcatcagg tcgcgcaggt aggaaacgta
     8881 ggccttggcc acctgcggcg ccttggcgcc ggtgccccga atcggccggg tgacgatctc
     8941 gatcagcggg gtgccggccc ggttgtagtc gaccagcgag tagtcggcgt cgccgatccg
     9001 gccggtgaag ccggtgtgca cgagcttgcc ggcgtcctcc tccatgtgcg cacgctcgat
     9061 ctcgatgtgg aagacctccc cgtccacctc cagatcgacg aagccgtcaa aggcgatcgg
     9121 ttcgtcgtac tgcgaggtct ggaagtcctt ggtcatgtcg gggtagaagt aattcttccg
     9181 ggcgaaccgg caccactgtg cgatgtcgca gttgagcgcc agaccgatcc ggatcgccga
     9241 ctcgatcgcc ctggcgttga ccaccggtag cgagccgggc aggcccaggc agacggggca
     9301 ggtctgggtg ttcggctcgg cgccgaaggc gttcgcgcaa ccgcagaaca tcttggacgc
     9361 ggtgttcagc tcgacatgaa cctcgaggcc gagcaccgga tcgaaagcca ccagggcgtc
     9421 gtcgaattcc atcaggctca tcggatgctc ccaacgttca ggtaggcccc ggcgggcgag
     9481 tcggcgaact ggtcgagcag gctggcgccc cagttgcggt tcagttcagc ctccagtgcg
     9541 gcaccaatcc ggtacagccg gtcgtcggcc agcaccggcg ccatcacctg gaagccgacc
     9601 ggtagcccgt tgtcgggact gagcccgacc ggaagggaca gcgcgcagtt gccggccagg
     9661 tttgacggga tcgtgcacag gtcggccttg tacatcgcca gcgggtcgtc gacccgttcg
     9721 ccgatcggga aggccgtcgt cggcgtggtc ggcgacacca ggacgtccac cttgtcgaag
     9781 gctgcgtcga agtcacgctt gatcagcgtc cgtaccttct gggccgagcc gtagtaggcg
     9841 tcgtaatagc cggacgagag tgcgtaggtg ccgatgatga ttcgccgctt ggcctcggct
     9901 ccgaaaccgg caccgcgggt caggttcatc acctcttcga cgctgtttac gccgtcgtca
     9961 ccgacccgca gtccgtagcg catggcatcg aaacgggcca ggttggagct gacctcactc
    10021 ggcatgatca ggtagtacgc ggccagggcg tactcgaagt tcgggcagga cacctcgacg
    10081 atctgtgcac ccaactgctc cagcactgcg agcgcctcac tgaaccgctg ctcgacgccg
    10141 ggctggtagc cctcgccgcc gaattcggtg acgacgccga tccgcatgcc ggccacatcc
    10201 gcgcgttcga ccgcgcccag cagatcgggc agcggtgcat tgatcgacgt ggagtcgcgt
    10261 ggatcccagc caccgatcac gctgtgcagc agcgccgcat cgcgcacatt gcgcgcgcac
    10321 ggaccaggcg tgtccagcga cgacgccatc gccaccacgc cgtagcgtga ggtgccgccg
    10381 taggttggtt tcacgccgac tgtgccggtg accgacgcgg gctgccggat cgagccgccg
    10441 gtatcggtgc cgatggccag cggcgcctcg aacgcggcca gcgccgcggc cgaaccgcca
    10501 ccggaaccac ccgggataag cgcgttgtcc cacgggttgt gggtggcgaa gtagccggag
    10561 ttctcggtgg atgagcccat cgcgaactcg tccatgttgg tcttgccgag cacgatcacg
    10621 tccgcggcca gcagcttgtc ggtgatcgtg gagttgtagg gcggcatcca gccttcgagg
    10681 atgcgcgagc cgcaggtggt cggcgcaccc tgcatggtga agatgtcctt catcgctagc
    10741 gggacgccgg ccagcgggcc gagcttctcc cccgcggcgc gcttggcgtc cacggcatcg
    10801 gcggacgcga gcgcccgctc gccgtccaca tgcaggaagg ccttcacccg gtcgtcgacg
    10861 gctgcgatct ggtcgaggtg ggcggcggtg acctcacggg cgctgacttc gccggacgcg
    10921 atcaggccgg ccagctcagt ggcgccggcg cgcagcagcg cgctcatgcc tcctccccga
    10981 ggatgcgcgg cacgcggaac cggttgtctt cggttgcggg tgcggccgcg agcacagccg
    11041 ccaccggcag cgacggcgtg accacgtcgt cgcgaaacac gttggtcagc ggcagcgcat
    11101 gcgacgtcgg cggcacgtcc gccgtggcca cttcggtgac cgtagccacc gattgcagga
    11161 tcacgtcgag ctgtggggcc aggtgcgcca gctcgtctgg ggtcagccgg atgcgcgcca
    11221 actcgccgag acgaccgaca tcgccagtgg tcaaagccaa ggtgaactcc tcctgcgatc
    11281 aagcggccgc gccgcgcgag ccctcagctt atcggcgccc ggtgacaccc ctgtaacacg
    11341 gatgagcgac actaggccgg tgttcttgct gcgagtggtg ctgcctgacc gtcccggttc
    11401 gctgggcgcg gtggccaccg cgatgggcct cgtgcacgct gacatcaacg cggtagagat
    11461 cgtcgagaag tacgagggtt tcgcgatcga cgacttcatg gtggagttgc cggcgacggt
    11521 cgcggccgat tcgctgatca ccgcctgcag cacggtcccg ggcgtcgagg tggtgtggct
    11581 ttcgcactat cccgagtcgt ggggtctgca ggccgacgtg gatgtgctca atgccatgac
    11641 cgaggatccc gagcacgccg aggagatcct caccctggaa gcgcccgcgg tcttccgcgt
    11701 gtcctgggcg gtcctcgtcg atcgacgcac cggcgaggtc ctgatccaga ccgagctggc
    11761 acctgacctg acgcccgcgg acgtcgccgg cttcggggcc ctggatcaac tgcatgacct
    11821 ggaactgccg gagggttggc tgtccggctg gggcgacacc atcatcgcgg tcgcgccgtt
    11881 ccgcaccgac gcgacgatcg tgctggcccg ccggggcggg cccgagttcc tgaagtctga
    11941 gctggctcgc ctcaaccacc tggcggcgct ggctctctag cagcctgcgg aacgagcccg
    12001 ggtcagaacg atggcccggc gatctgggga tttgtcgatc tgccgcaacg ccgtccgtcg
    12061 tggaggtgag gaggttccaa tgatcaccaa ccatgtcacc ttcgtcctac tgaccgagca
    12121 acgctcgcag ggtcggcaaa ccccggaacg accggtcgag acgggacgta ccagggcact
    12181 ccggctgccc gtgcccggct cttggcaatc tctgggttcc gcccgcgcga gcacaggatt
    12241 ccgccgacac cgattgggcg gcgatcctcg cgctgtacca ccaactgccc gacacaccgt
    12301 tcatcgagct caaccgcgcc atcgccgtcg ggatggccgt cggcccggaa gctggactcg
    12361 gcgcactggc ggagctcgcg gcgtccggtg cgctggccgg acaccacctc ctgccggcgg
    12421 cccaagccga cctgtgccga cgctgcggcc gcgtccgcga agccgaacgg cactaccgtg
    12481 aggcgatcga actcgcgccc accgatccgg agcgccggtt ccgacaacgt cggctcgcgg
    12541 cgctcggtac ctgagccggc tgactcgtta cgtccgagcg caccgctact tcacccaaag
    12601 ccgaactagg gtgtgttcct cggcccgctg gcgcggccct cgtcaacacc atcctgcgtt
    12661 gaccgggaac tgcgttcccg ctcaacgcag gatggtgtac atccagtagc cgtagccgac
    12721 cacggcggcc agcacgccca acagggcgag cagggtccac cagcgcatgc ggcgcttctg
    12781 ccgggtggtg ggcacctcct gcgggcccca ctcccccatg ccgggacgca cgcgcggcgg
    12841 cgcgccgaca ttcaggttca ggtcggagtt caagccttcg ggcacgtcca gatcctagtc
    12901 ggccgtgatc agaccttccg gaccggcggt gagcagtcgc tcgaaggcgt ccgcttcgat
    12961 caccggaacg ccgagagcga tcgccttgtc cagcttcgaa cccgcgctct cgcccgcgac
    13021 cacgtagctg gtccgcgagg acaccgagcc ggcagccttc ccgcccctgg cctcgatcgc
    13081 agcggtcgcg ccatcacggc tgtagccggg caccgcaccg gtgaccacga tcgtcagccc
    13141 ggccagcgtc tgcggctcac ccggggcctc cgcgcgtggc tcgtcggcca agcggcagcc
    13201 ggccgcccgc cacttggcca cgatcgcgtt gtgccagtcc accgtgaacc attcggcaat
    13261 ggacgcggcc agcgtcggcc cgatgccgga caccgcgctc agctcctcga ccgaggcgga
    13321 cgccagcgca tcgatgctgg ggaacgcggc cgccacatcg ggcgccactc ccttgcccac
    13381 atgccggatc gacagggcca ccaggaagcg cgcaaacggc cggctcttcg cgtcatccag
    13441 gttcgccagc aacttgcgtc cgtttgccga gagctcgccg gctttggtgg tgaacacccc
    13501 ggcctcgacg agcttcgccg cggtcaggtc gaacaagtcg ccctcgtcgg tgagcagacc
    13561 ggcgctcagc aacgcgtccg cagactgcca acccagcacc tcgatgtcca gcgcctgccg
    13621 cgaagccagg tggaagagtc gctcgcgcag ctgcgccggg caggaccgct ggttggggca
    13681 gcggatgtcg gcgtccccct ccttctccgg acgcagatcg gtaccgcaag ccgggcaacg
    13741 cgtcggcatc acaaatgccc gctcggtgcc gtcgcgcagt tcggccaccg gaccgagcac
    13801 ctccgggatc acgtcgcccg ccttgcgcag caccaccgtg tcaccgatca gcaccccctt
    13861 gcgctgcacc tcgctggcgt tgtgcagggt ggccatctcg accgtcgagc cggcgacgaa
    13921 caccggctcc atcaccgcgt acggggtcac tcgtccggtg cggccgacat tcacccgaat
    13981 atcgagcagc ttggtggtga cctcctccgg cggatacttg taggcgatcg cccaccgtgg
    14041 cgccctggag gtgtggccca gcctcgcctg caactcgcgg tcgtccacct tgatcacgac
    14101 cccgtcgatg tcgtgttcca ccgagtgccg gtgttcgccg tagtgggcga cgtattcctt
    14161 aacctcggcc acgcggtggc agatgcggta gtgggcactc accggcaggc cccagccatg
    14221 cagcagcttg tagccctgcg actgacgttc cagcggcggc ccagagaact cgccgatgcc
    14281 gtgcaccagg aagtcgagct tccgagacgc ggtcacccgc gggtccttct gccgtagcga
    14341 accagccgcc gagttccgcg gattggcgaa cggcgccttt ccggcggcga ccagaccttc
    14401 gttcagccgg gcgaagtcgt ccggacgcat gaacacctcg ccgcgcacct caaccagctc
    14461 aggcactgcc tccccggtga gctgggcagg aatcgaggca attgtgcgga cgttggcggt
    14521 cacgtcctcg ccgaccgtgc cgtcgccacg ggtcgcggcg ctcaccagcc gcccgccccg
    14581 ataaaccaga tcgagcgcga ggccgtcgat cttcagttcg cagaggtagc cagcggtagc
    14641 tggatcggtg ccggccagcg ttgccgcacg ggtcagccac ttgtccagtt cctcgagcga
    14701 gaagacgttg tccaagctca tcagcggcag caggtgggcc accggggcga aggtcgcgct
    14761 cggcgcaccg ccgacctgct gggtggggct gtcgggcgtc cgcagctctg gatgcgcctc
    14821 ctcgatcgcg gtcaactcgg ccatcaaccg gtcgaactcg gcatcggcga ccgtgggcgc
    14881 gtccagcacg tagtagcgcc agcggtgatc ggtgatcagt tcgctcagct cagcgtgcct
    14941 ggcgcgggtg ccgtcttcgg tcatgcgcgc aatctagcgc gaggctccga cactgcgaca
    15001 ccggtcggca cgggagtcga aatgtggcct gtcgcggctg caggagggct gagaacccgc
    15061 actttgatgg ccgagcggct acccgccgct gagctgttcc gcaagctgag tggacgcgac
    15121 gttcagcacc tcgaaacccc ggctgacctc ggcgctggtc cagccggcaa ggccgcaggc
    15181 tggagtcaac accaaccggc ccgagattcc agagtccgca ccgatgcgtt ccagcgcccg
    15241 cagcgcacgg acgcgcgtcg cgtcgacccc cagcggacgg gggctggcgg acgtcggcag
    15301 gcagcccaga tagagctcgg cgccagcgtc cgcggctgct gccaacgcgt cccagtcggc
    15361 ggttttgagc tggtcgaggt cgagcgagat gccgtcgaat cccgcgggcg tgctatcgcc
    15421 gtgcagtagc gatcgcagcg gccagccgcc cggccgctgc cagggggcgc aactgtgcaa
    15481 caccgtccgc accgcaaggc ctcgtcgcgc tggttcagcg gtcagcaggc cgagcgaccg
    15541 gcagacttcc gggaggtcca ccgctcgatg ccggaagtag ccgcccggcg tcggcaccgc
    15601 cccggcggcg accgctggca aggccggctc gtcgacctgc aggatcaggc gcgcaccggg
    15661 taggcgtttg cccaggtcaa ccagcagttc accgattccg tcggccagtg cgccggccag
    15721 gtcgcggcgg gcgccgaggt cggcgagcac ccttccggtg tgcgccaccg cgagcgcggc
    15781 cgaaagcgtc cacggccccg cgaccgccac cttgaaccaa cccgtgtaac catcaccgat
    15841 ctcctccagg cggtcgagat catcacgcca ggtggctcgg gcacgccgct ggtccacccc
    15901 gggcgcgtcg gtcaaccgcc actcaccggc ctcaaggctg gccggcagcc cgctgggcag
    15961 gccgagcccg cgtccgatca tcgcggccca gggaccgcgc gcgggcagct ccggaaggta
    16021 gggcagctcg ggcaccttgt cgaaggtcat tcgcagggcc gcaccgaagt cggtaccggg
    16081 tagcgagccc aagccggtga aggcgaccat caggccgccg tagcggagat cgttgcgctg
    16141 ccgaccacgc ggtcgccgtc gtagtagacc gcggcctgac caggggcgat accaagagcc
    16201 ggctggtcca ggacgatgcg gacgcaggca ggcggcggcc cttcgtgacg ctttcctcgt
    16261 tcctcggaaa gctcctccgg gattgttacg acgtggatgg tcgcaggcat cgcctcgccg
    16321 tgggcgcgca cctgcaccag tccgcgccag gcgcccgcca cgaaaccctc cgtccaggtc
    16381 ggccggatcg cttcgatcgt ttgcaccgca aggcgctcgc gcgggccgac ctgcacggtg
    16441 ttcgccaccg ggtcgatccg cagcacatag cgcgggcgtc cgtccgctgc cggcaccgaa
    16501 aggtgcaggc cgtgccgctg tccgacggtg aactgctgat accccgagtg cgcgcccacc
    16561 acgtctccgg tctcgtcgac gatgtccccg acttgcgaac ccagccgcga gcgcaggaaa
    16621 ccggcggtgt cgccggtcgg gatgaagcag atgtcgtggc tgtccggctt cctggccact
    16681 aacagcccgc gcgccgcggc ttctgcccgc acctcggcct tcgcagtgtc ggcgccgagc
    16741 gggaagagcg agtgccgcaa ctggtcggcg tccaggacac ccagcacgta ggactggtcc
    16801 ttggcgggct cgggcgagcg atagagcgaa accgtgtcgc cacttcgctc gatccgggca
    16861 tagtgcccgg tcgcaaccgc gtcgaagccg agcgcccggc cgcgttcgag caccgcggcg
    16921 aacttgatct tctcgttgca gcgcaggcag gggttcggcg tgcggccggc cgcatactca
    16981 tcgacgaagt cggccaccac gtcggcagcg aagcgttcgg cgaagtccca aacatagaag
    17041 gggataccga gggcgtcggc ggcccggcgc gcgtcgtgcg agtcctccag cgagcagcag
    17101 ccgcgggcgc cgctgcggta ggcctcggag ttcttggcta gcgcgaggtg gacgccgacc
    17161 acctcatgtc ccgcgtcgac caggcgcgcg gccgcaacgg cggagtcgac tccgccggac
    17221 atcgccgcca gtacgcgcat gcggtcttcc ttccaggtct tcctcggacg gctggtggct
    17281 cgaccagccg ggcagatggc cccgaggagt ctacgtcgcg gacgctgctc gcgcccgtgc
    17341 caccaccccg ggcagcagct tcaccagcgc ttcgatctct gctcgcgtgg tcgtcggtcc
    17401 gaacgagaac cgcaggctgg cggacgcgtc cgcaacacct cggcccatcg ccagcagcac
    17461 ctcgctgggc tggtggacgc cggccgtgca cgccgagcca gtcgacgcgt cgatgccggc
    17521 ggcgtccagc aacagcagca cgtcatcggc ccgaacgtct tcgaaggtga ggttcacgat
    17581 cgcgggcgag cagggggcca cggtgttagc gcggcagccg gccacgccgg ccagggcacc
    17641 actgatcaac aggttgcgca actcaccgag ccgctccgcc tccgcggcct gtcgctcgat
    17701 cgccacccgt gccgcggtgg caaagccaac cgccagcgcc accggcaccg accccgaccg
    17761 caagcgcgcc tcctgcccgc cgccgaaccc ggtcggccgc agtttcaccc cgcgtcgcac
    17821 caccagcgcc ccgatcccaa ctggggcgcc gagcttgtgg ccggacaccg tgagcagatc
    17881 gaggttgctg gccgcgaaat cgatcggtag gtgaccgaag gcctgtaccg cgtcgctgtg
    17941 cgcgagggac ccggccgcgt gcgccgcgtc cgccacctcg gcgaccggct ggatcgcgcc
    18001 ggtctcgttg ttcacccaca tcaccgaggt gagtccgacc cggtcggtca gcaactcggc
    18061 aaccaccgcc ggctccacgt gacccgacgc tgccaccggc agggtgaccg tctccagccg
    18121 cgtccgcgcg gcagccaccg aagggtgttc gatcgccgac agcgccacca ccgggcggcc
    18181 gtcggtggcg ccgcccagca gagccaggtt gttcgcctcg gtaccgcccg aggtgaagac
    18241 cacctcggtg ggcagtgccc ccaggtgatc cgccagcgac tcccgcgact cctcgagcag
    18301 gcggcgcgcc gctcgcccgg acgcgtgcag cgagctcggg ttgcctgcca gcggcagtgt
    18361 tgcggccaca gccgcgacca cctccggcag ggctggcgag ccggcggcgt ggtcgagata
    18421 gctgcgactg gtcaaatgtg cgcttcctga atcgttgctg aatcgttttc gcgtatgcgg
    18481 gcgcgcttta gtgcaccctg actcaggtta ggccacactg ttgtgcgaac tattgcagga
    18541 gattggtcaa tgactcagtc cgaacagact cacccgagcg gcgacacctc cctcggtgca
    18601 caggtcacag cggagggcac gctgttctcg ctgtgggcgc cgcgcgccac ccgggtggaa
    18661 ctcgccctga tcgacgagca tcgtcggcag acgaaccacg acctggttgc tgacgatgcc
    18721 ggagtctggg aagtattcgt ccctgcagtc ggggccggac agcggtacgg gtaccgggtg
    18781 catggcaagt ggtcgccgga gaccggcgca cgattcaacc cggccaagct gctgctcgac
    18841 ccctacgccc gcgcgatcac cggcggcgtc gactactcgg gcccgatcac cgaccacacc
    18901 ccggggtcca acttcctgcc cgatcccacc gactcgttcg ccgccgtccc gctgagtgtc
    18961 gtggtggccg attccgacgc gccgcgtccg ctgtcacgtc cgttgcgcga atccgaactg
    19021 gtcatctacg aagcgcacct gaagggctac acccagctgc acccggcggt gcccgaacac
    19081 ctgcgcggca cctatgccgg catggcgtat ccagcagtca tcgaccacct gctgaagctc
    19141 ggtgtcaacg cggtcgagtt cctcccgttg caccacttcg tctccgagcc gttcgtggtc
    19201 ggacgcgggc tggtgaacta ctggggatac aacaccctcg gctttttcgc tccgcacgcg
    19261 gcctactcgt cgtccggcac cctcggcgag caggtgtccg agttcaagga gatggtctcc
    19321 gcactgcacg attccggcat cgcggtcatc ctggacgtgg tctacaacca caccggcgag
    19381 ggtggccacg acggcccgac ccttagcttc cgcggcatcg accacgccgc gtactaccgg
    19441 ctgaccgacg acaagcgcaa cgactacgac gtcaccggct gcggcaacgc cgtcgacacc
    19501 tccaagcccg gagtgctcaa gctggtactc gactcattgc gctactgggt caccgagatg
    19561 ggtgtggacg ggttccgttt cgacctgcag accacgctta tccgcgacca cgcccaccac
    19621 gtcgaccaga accacgagtt caagcgactg gtggccgccg accccgtcct ggccggaaag
    19681 gtgatgattt ccgagccgtg ggacctgggc ccctacggct accaggtcgg acgctggggc
    19741 aagggctggt cggagtggaa cgaccgcttc cgtagcttcg cccgcgactt ctggcgcggc
    19801 gccatcccgg gcgtgcagga gctggccacc cggctgtgcg gctcctccga catctacgac
    19861 cacggcggac ggccggcgac ctcatcggtg aacttcgtca ccgcgcacga cggcttcacc
    19921 atgcgcgacc tggtcaccta cgacctcaag cacaacgagg cgaacgccga acgcaaccgc
    19981 gacggggccg acgacaaccg ttcctggaac cacggctacg agggcgagac cgacgatccg
    20041 acgattatcg cgtcccggca gcgcacggcc cgcaacatga tggccaccct gctgctcagc
    20101 gcgggcatcc cgatgatcac cgcaggtgac gagatgggcc gcacccagat cggcaacaac
    20161 aacgcctact gccaggactc gccggtgtcc tgggtgaact ggtactccca ggatgactgg
    20221 agcgagcagg aggaactcac ccgagcgctg ctgcagcttc gcgctgacta cccgctgctg
    20281 cgaccagagg gctggcgatc ccacggcgag gtgacggacg gatccggtca cggcctgggc
    20341 cgctcccaga tcgcctggtt cgccgagagc ggcaccgaga tgaccaccga gcagtggcac
    20401 gacccgggtc gccgtaccct cggcgaatac ctgtcggacg gtagtcaggc gctgctcatc
    20461 tacttccact ccggctggga gccggtcgag ctgtcgctgc cgggtgcgcc atgggcactc
    20521 gcttaccgca tcctcgccca caccggtacg tccggcgagc tgccgcgaaa gcggctctcc
    20581 cccggatcga cggtcacgct gccggcgcgg acggtggtcg tcttcggtgc cgaggtgctt
    20641 accggcgtcc gcccggtgcc ggtctcgccg gtcaccccgg acgaggccga agccgacgtt
    20701 gcggacgctc ccgacacggt cggccaagac ctccccccga acggaatccc aaacgctagg
    20761 ttggggctgt gagcgccacc gtgaacccgc aacgcaagcc gaagcccgcg ccgaagggtg
    20821 ccgccccgaa ggttgcggcc agcaccgcaa caccggccgg caaacccgtc aaggcggagc
    20881 ttgagctcaa cccgccggtg actgtcccgc gcggcttcgg ccgcatcccg gtgaccgatg
    20941 tctacccggt catcgaagga ggcgcctatc cggccaaggc cgtggtcggc gagctgatcc
    21001 cgatccgtgc gaccgtgttc cgggagggcc acgacgcggt caacgcctcg gtcatcctca
    21061 ccgcgccgga cggacgcgag cagcgtgtcg acatgacccc gatggagccc cgaggcctcg
    21121 acccgtggga agcctgggtg cgtcccgacg ccgagggcgc gtggaccttc cgggtcgagg
    21181 gctggtccga tccgtgggca acctggctgc acaacgccga ggccaagctg cccgtcgggg
    21241 tcgacatcga gctggtctgc ctggagggcc gtgacctgct cgaccggacc gccgcgtccg
    21301 ccaaggccgc gaatgacccg gtgcaggcct caatcctgca cgcgaccgcc atgctgctga
    21361 ttcccgagcg tccggccacg gacctgatcg aggtcgccac ggccaaggga atctcgcgcg
    21421 cgatggagca gtacggcccg cgcgacctgc tctcggcaac cgcggacttc ccgttgttcg
    21481 tcgaccgtaa gcgggccctg ttctccagct ggtacgagtt cttcccgcgt tcccaggggg
    21541 ccaacttcga tccggcgacc aagaagtgga cctcgggtac cttcgacggc tctcatcagc
    21601 gactggaaga agtcgccgcg atgggcttcg acgtggtcta cctgcccccg atccatccca
    21661 tcggacgagc cttccgcaag ggccgcaaca acacgctcac ccccggaccg aacgacccgg
    21721 gctcgccgtg ggcgatcggc gcggccgagg gcggacacga cgccatccac cccgacctcg
    21781 gcgactggga agccttcgac cgcttcgtcg cgaaagccac cgagtgtggc ctggagatcg
    21841 cgctcgactt cgcgctgcag gcgtccccgg accacccctg ggtgaaagcg cacccggagt
    21901 ggttcaccac ccggatcgac ggcacgatcg cctacgcgga aaacccgccg aagaagtacc
    21961 aggacatcta cccgatcaac ttcgacaacg atcccgaggg catctaccac gag
//
LOCUS       DAOPRQ010000044        20618 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_2186_length_20618_cov_13.309926, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000044 DAOPRQ010000000
VERSION     DAOPRQ010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 20618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20618
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2186_length_20618_cov_13.309926"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            39..449
                     /locus_tag="PKJ61_07175"
     CDS             39..449
                     /locus_tag="PKJ61_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13644.1"
                     /translation="MDLPDGYLDALSDELAPYGFEFGATTPDEDGGLALRYQADPESF
                     AREFPQAGVEDSYGVHWPPPHLALWLRFDRHGDPYEISFETFDLLADTASNDPQLNAR
                     LNTLEDPSDQASAIGEALYAALHPQHHEYSDYLE"
     gene            complement(491..616)
                     /locus_tag="PKJ61_07180"
     CDS             complement(491..616)
                     /locus_tag="PKJ61_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13645.1"
                     /translation="MKKVLRAVLVLAGAGYVAWKYWQTSNQDAAAVWAAETDRIG"
     gene            complement(630..1538)
                     /locus_tag="PKJ61_07185"
     CDS             complement(630..1538)
                     /locus_tag="PKJ61_07185"
                     /inference="COORDINATES: protein motif:HMM:NF015755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HOC13646.1"
                     /translation="MTEGTQARAVGRSWLDRLAIVLLIGATAVSVGAVALLTVYLNRV
                     SDASSEIDRIGSLGSYSGRPSAVVVDGVNAVNYVLTSTSADALDALVVAHLSASRQQL
                     TLIAFPIDLQGSSGSLASTNRRDPLLVMRELERMTGARMDHLVQIDRDRFAHVVDALG
                     GIELDGDLVSGAQAVERLSGIPAHDRSLASAEILRATLVRASAGSAITEPTRFDRVLN
                     ALTPCLKLDESLTTDEIQAILVESKVHADEIATIQVAVTRSGARAVLAEERSMDALRQ
                     AFATDDFALANPSLAVATQASAEPTK"
     gene            1628..2038
                     /locus_tag="PKJ61_07190"
     CDS             1628..2038
                     /locus_tag="PKJ61_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015068909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13647.1"
                     /translation="MPAENQRQADARRRHELAAAAVAAEAAAAQVQLDAFVARLTAAS
                     IDPEPLQATLLNGSRVKTDKVGWYLNAAQTLAIGPDGTYYQLVTSGGPLARLSGVRIG
                     PGTPTLVIGRGGRDGETGDLADFIDRAWARYAAE"
     gene            2325..2978
                     /locus_tag="PKJ61_07195"
     CDS             2325..2978
                     /locus_tag="PKJ61_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13648.1"
                     /translation="MAVSGVLGALEAEWSRFADGSSTRVLRWAELDRALLGCRGVDAV
                     LARIRREPDAVLAALLRIGQGGDQLALLVVVKAMLGKLVLLSAGRPDVLIEAISEMWL
                     ATAEYPVARRPRSIAANLSWTVSRRIRASPPRTSAPLTVEPAAATPDPDASTTLADAR
                     RLGLIDELTHRTLWAVYVAGLTSYQAAAVLDTTPDLVRWRCSRAIRRLREQAELLAS"
     gene            3115..3882
                     /locus_tag="PKJ61_07200"
     CDS             3115..3882
                     /locus_tag="PKJ61_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015071897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOC13649.1"
                     /translation="MATALVTGATAGIGAAFARALAERGHDLVLVARNRERLAAKAAE
                     LEALGVRVEVLPAELADRAQVATVAARLEQAEQPIEILVNNAGFGLKAKLLDVDVREH
                     DQAFDVMCRAVLVLGGAAGRAMSVRGHGQIINVASLSAWITQGNYSAVKAWVKVYSES
                     LANELHGTGVTVTALCPGWVRTEFHDRAGIRTGSIPDWIWVDADRVAREALDHAARGR
                     VLSVPTKRWQVARFAMQLAPRPLVRAASRMLTKSRGQ"
     gene            3936..4814
                     /locus_tag="PKJ61_07205"
     CDS             3936..4814
                     /locus_tag="PKJ61_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOC13650.1"
                     /translation="MADSGTGSYSAAAPAAARLVAQHLVMKPYIWSALSVHTHGMRNL
                     ETLKPPFIVIANHSSHLDAPLIYGALPRRLSRNLAAATAGDYFFNAWYKALPTKLFFN
                     SFPVARQGHANRAGQRGLAAHLVNDGVPILIFPEGTRSRTGAMANFTPGAAALSISRN
                     VPVLPIALVGAYAAMPYGANVPVPGRPHVHVVIGRPLRAAPSEIAHQFSERLHRYVVE
                     MHDTTARAYGMPTQADFARAVALRTAAAQADEAQKAPVIELSRPNDAQSAVVEPVPEP
                     SPETEPTTEDPPAKAG"
     assembly_gap    4820..4919
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4920..7135)
                     /locus_tag="PKJ61_07210"
     CDS             complement(<4920..7135)
                     /locus_tag="PKJ61_07210"
                     /inference="COORDINATES: protein motif:HMM:TIGR03061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HOC13651.1"
                     /translation="MKAELVNSTPAKWYALLGLILVPLLVAGGFLFAGVNSDSRLHTV
                     QAAVVNLDEPVELDGQYTPLGRQLTANLVDSDRQENLTWVLADEADARAGLTTGEFAA
                     MVVIPENFSAAATSFAENEGDQAKQATIQVHTSPVAGVADATLGKLVAAEAAKMLNQM
                     LTESYLDQIYIGFNDMGEQFVTMADGATELADGAEKLADGIGEAADGSKQLYTGARAL
                     ANGLDTMHTKTADLPSGTRQLADGLGTLHKKTKELPEGTRQLADGLAQLETGTKDLPA
                     GSKELSSGLTSYVAGVSTLIDQTVNSLDGQVALAGGIKQLSQAASGLNGGLKAYQSQL
                     EKLGSNGDLVAGAYQGALASSEGKGLLAACGGDGGCEDLVRNAMQVGAGAGVAAGGKA
                     AAAGLEAKQNGVSLLDVAGGLDDGLGQIAGQLSGVPSPDEVAAQKKQLAQLKAGGTQL
                     DQGLQTMAAGLPALASGVSQLAGGADQLADGMPALVDGIGKLADGADELADGLPALVD
                     GIGQSADGAGQLADGVDELSAGLISAADGGTKLADGMREMSDGIAEGKDKLPSYTESD
                     RETLATVVASPVQTDDLAGLANANVGWISLVLVLALWLGALAVYAVTKAIAGGLLSSS
                     EPTAVLIGRSLLPGVVVVGIQALVVSGLAQFGLNVSPQKWLAITAVLLLAGVTFVVLN
                     HALVSWFGGVGRLVAIGFAVLTTADALTSAAPGLFEALRPFSPLTPALDAIRAIVT"
     gene            complement(7132..9936)
                     /locus_tag="PKJ61_07215"
     CDS             complement(7132..9936)
                     /locus_tag="PKJ61_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOC13652.1"
                     /translation="MSAYLYRIGRACYRLRGRVVGVWAIILVLFGGLALAVGGDFDDV
                     FEVPGASSTKALAQLKVTFPEAADASATVLITAPDGKKLTDADVTAAIEDWTDRMDAL
                     PYVRGVIGPFSEHVDGLISEDGTSGRANVRVEGQASTITDAQREELTETGQTLEKTLP
                     GSKVLMGGEVFSIHVPKISAVEALGLGVAVIVLIATLGSVVASMMPVGTAIAGVGLGV
                     LAVQIASGVITISSTTLILAVMLGLAVGIDYALFIISRHRDQLATGMDVEESAARAVG
                     TAGSAVVFAGLTVIIALIGLSLAGLPFLTVMGVFGSLTVAFEVVLALTLLPAFMGFAG
                     ERLRPKSKTRGSRGVQGEASETGAALDQPPSSSSGVVPSGSGAVPLNSGATEPPIKRK
                     FSASTWWVNVVTKWPLVTIAVVLVALGGLAYPAKDLQMALPTSGRSLPGTQDRQTYDA
                     ITETFGVGFNGPLIMIVGIVESDDPVEVLDGLKAEIEAMPGVKMVAAATPNANVDTGM
                     VQIIPTTGPDDPATAALVEQLRERQAEWQQRYDVNTEITGFTAIQIDVTNRLSAALVP
                     FGVFVVGLSLVLLALVFRSIWVPIKAALGYLLSVAAAFGATTLVFNQGWFKQLINLPE
                     AGPVISFLPIILMGILFGLAMDYEVFLVSRMREEYVHGNVERSVEDGFVHSSKVVVAA
                     ALIMFSVFAFFVPAGEGAIKPIAFGLAVGVALDAFVVRMTLVPAVMKLLGRHAWWLPK
                     WLDRRLPALDIEGEALTRQVHLAQWPSPDDTSAIVGEGLTARTDRRTLFTDLALSVQP
                     GEVLVIEGEHAQRRALLLAIAGRVKLTEGKLKVLGYVLPEESPVLRRQAPVLGPTVPD
                     FKKVLTKQAGGLVCVDSADELSSSRDAALLAALAASAKAEEPTTWVLGVMPGSDLEAQ
                     LPVPFRVLKLTSHLALEGAK"
     gene            10042..10674
                     /locus_tag="PKJ61_07220"
     CDS             10042..10674
                     /locus_tag="PKJ61_07220"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOC13653.1"
                     /translation="MDNHPTLTPRRVQTREKLMDAAVSLFAEKGVLGASVEEICERAE
                     FTRGAFYSNFASKDELCLAVMNRQAERDLAAAARTVAGLVDDMTAPTSLDELIERAVG
                     VFLATQPRDPEDLVVSMELRLYGVRNPKLREPLLALTDEVAAELGGIMAQALTRERAR
                     MTVPMSQAIDLLHAVYVHSGITALLNGLEPDDESRRRQLTVLLRSLVTVD"
     gene            10743..11720
                     /locus_tag="PKJ61_07225"
     CDS             10743..11720
                     /locus_tag="PKJ61_07225"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002523841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HOC13654.1"
                     /translation="MTATANEYPDLNSSKLAIVGAGSVGTSIAYASMIRGSARQIALY
                     DINTKKVEAEAADLAHGTQYTPTSQVTGGSDIEVVKDSSIVIITAGARQHPGQTRMDL
                     AAENTRILSELMPRLLDQAPNAIYVLVTNPCDVLTVVAQKVSGLPPNRVFSTGTMLDT
                     ARVRWAIAKRARVAQSNVHAVIMGEHGDTEFAIWSSASIAQVPIRKYRDEAGHRVFSE
                     EILAEIAHDTTHAAYSIIEGKGATNYAIGLTGARLTEAILKQQRKILPLSSVLTDYRG
                     VSDVALSVPSMVTEKGIERVFEVPMDAHEIALFQNSARHVQATCRELGF"
     gene            complement(11995..12966)
                     /gene="glsA"
                     /locus_tag="PKJ61_07230"
     CDS             complement(11995..12966)
                     /gene="glsA"
                     /locus_tag="PKJ61_07230"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR03814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HOC13655.1"
                     /translation="MSDDFVSTGRLAPSPDVRRALAATYADVLNNRTGANSPVYPALA
                     DADPEKFGLCVVASDGRVFDEGDAFAGFAMMSVAKPFTFALVCAHIGAQRARDELGVN
                     ATGQAFNAVDPVLAASGGRTNPMVNAGAIATCSRLPGNGLEGRWAHLLTGLSAFAGRE
                     LAIDSALYESVSATNFRNREITAALHGQGALASAPEVALELYSRQSSLLVTAHDLAMM
                     GATLADGGRNPVTGAQVIPSALCHHVLAVMATAGMYERSGEWLYDVGLPGKSGIGGGI
                     VTVSPGKGGLGSFSPRLDEAGNSVRGVQAAARLASELGLGLFASRPV"
     gene            13108..13662
                     /gene="cobU"
                     /locus_tag="PKJ61_07235"
     CDS             13108..13662
                     /gene="cobU"
                     /locus_tag="PKJ61_07235"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HOC13656.1"
                     /translation="MTHVLVTGGVRSGKSSYAEALLDADAAVTYLTPGYPADAVDAEW
                     AARVAAHQLRRPPAWRTIESLNLAETLRAVDGPVLIDCLGTWLTRLFDAWDAWEDDSV
                     HHLGRLDGELSTLAAAVREHTSDVIVVTNEVGWGVVSAYKSGRVFADQLGRVNQAIGA
                     ACDQVVLMVAGRALMLPDGSGGPS"
     gene            complement(13701..14471)
                     /locus_tag="PKJ61_07240"
     CDS             complement(13701..14471)
                     /locus_tag="PKJ61_07240"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:NF014685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HOC13657.1"
                     /translation="MNLGAGFRLALGLLTIVPVRPPETIGREQARVAMLVAPLAVLPV
                     TLMASGLGFGAVALGLPGLLGGVAAVGAMAFGTRAMHLDGLADTVDGLGSGRDASGAL
                     EIMKRGDVGPMGTVALVLVLLAQVIASGALLERPYGWLVVAVLLAVSRFALLLGCGVG
                     LPAARPGGLGALVAESIPAAAAVVALAVWLAALQGLACVDGRAWWLPGIALIGAVVAC
                     TWLLLRSKKRFGGVTGDVLGAIVEVAATVLLVVWMIRA"
     gene            complement(14468..15502)
                     /gene="cobT"
                     /locus_tag="PKJ61_07245"
     CDS             complement(14468..15502)
                     /gene="cobT"
                     /locus_tag="PKJ61_07245"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HOC13658.1"
                     /translation="MLPSKTITVPSEFAYAAARQLSDGQAKPLGALGELEELGAWIAS
                     VQGTCPPRQLDDVRVVVFAGDHGIAADGVSAYPAAITPAMVYGIATGQAGVSALAAAN
                     GVRVSIYDIAVAVDFPDLPGEISRFKVRRSSGAIHLADALTRDEVEQALDFGDQIAAE
                     QTADGAQLLIAGDLGIGNTTIAAALIAATLGVPAAAVTGRGTGIDESALVHKTALVQA
                     ALDRVGARASDPVERLIALGAADFAAAVGFITGAARRGVPVLLDGVISVAEALVAEDL
                     APGTKAWLRAGHRSTEPGQALGLDRLGLKPLVDFSMRLGEGSGAALAVPMLRSAVAAL
                     TGIAQLADLT"
     gene            15542..16081
                     /locus_tag="PKJ61_07250"
     CDS             15542..16081
                     /locus_tag="PKJ61_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HOC13659.1"
                     /translation="MSGLDDVGRLWVGDSTTERDAVLTLLVGLAILVGLVGIVIPVLP
                     GSILIGLAVLVWAVMTGTVLSWWVFAAVALLLVVGSVITWVITAHHTRAAGVPNGSLV
                     FAGLAGIVGFFVIPVIGLLLFFPLGLFAAEYWRLRDPGAARASALVGLKATGLGMLTE
                     LGLALAAAAVWLVAVLNGV"
     gene            16142..16648
                     /locus_tag="PKJ61_07255"
     CDS             16142..16648
                     /locus_tag="PKJ61_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008363880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HOC13660.1"
                     /translation="MASKSRFTLPGLSQEKASKVVKILQERLNSYNDLQLTLKHVHWN
                     VVGPSFIGVHEMIDPQVDQVRLDADEVAERIAALGASPLGTVGAILTTRDWDDYPLAR
                     DTVPSHLTALDKEYDRVISENRQAIEDVEDLDLVTQDLLIGQTGALEKFQWFLRAHLE
                     TSSGTIPN"
     gene            complement(16710..17225)
                     /locus_tag="PKJ61_07260"
     CDS             complement(16710..17225)
                     /locus_tag="PKJ61_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOC13661.1"
                     /translation="MSRLVLTVVGDDRAGLVAALAEVVSAHGGNWEDSQLAELAGTFA
                     GIVVVSVPDDQAVAFTSALAELDGLLKVTAYPGAAADAPGHWQGLSLSVLGNDHPGIV
                     RDISAALSRHQLSIESLTSQTMDAPMAGGRLFEAQVVARVPEGADLGEIRAELERIAS
                     EILVDIALTDD"
     gene            17316..17714
                     /locus_tag="PKJ61_07265"
     CDS             17316..17714
                     /locus_tag="PKJ61_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6394 family protein"
                     /protein_id="HOC13662.1"
                     /translation="MNSEKVVFGFFVLLAATLNFGYVTGEIGNPLHHNAIELYAAVVV
                     NLIATVLKLGDRTQMGAVHLASSLVASIQLLIAAGIWIWETQGLGVDPLQVGERAMSG
                     VVSLALGALLANIVSVVLMVIETANFRRRA"
     gene            17717..19438
                     /locus_tag="PKJ61_07270"
     CDS             17717..19438
                     /locus_tag="PKJ61_07270"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HOC13663.1"
                     /translation="MADRPDVRAESATELRQAEIALPETSASTDAIFILMRRMRSPLV
                     VLLIVFAVDVLGLSLMPGVDASGAPHRMTIFDAFYVMSYTATTIGFGEIPYEFSIAQR
                     MWVTFSIYSSVVVWAYSLGTLFSLFQDRGFREAVQLQRVAGQVRRLREPFLIIAGYGQ
                     MGQRIAETLDAQGRRVVVVDSDDHVRDQLAGGKLSIDVPAIDGDIRDPRTLTLAGLGH
                     PHCAGVLAMTHDDSVNLAAVMAVRLLRPELPVYARANDRVTAASMHDFGADGVINPFD
                     RFGEYLILRLQRPVTYRLVKWLLAAYDSPLEECRTRLPSGRWIVASDDHFGEEIASDL
                     RRAGVVVEVVDAQESLPNLRDVAGLVAGAADENRNLALAGSARMSHPGVFLAVRACSE
                     GLGPLLDAFSPDIVFIPARLTAEEALARVVTPDFWDFFRFAFNQDEEWSRELLDRLVA
                     RQGTGSPTSRRIVLDETHAPGVLKWLTRHSLNLGDLLRDPDDREQPLALVPLIVTREC
                     VDTFAPADDFALAPGDELVVAGHDAAMDVLDRTLFHPDAVEYVATGNVVPATWIFRKL
                     SPRVQDA"
     gene            19666..20607
                     /locus_tag="PKJ61_07275"
     CDS             19666..20607
                     /locus_tag="PKJ61_07275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13664.1"
                     /translation="MNTDPSALIGSVAPILVLIVLGALLARTSVLDDATVAGLKRIIV
                     NVALPAVLFTTFLKTRFEPEHLWLVLLVFGLCVALLTLGRVLPVGPPSRYRPFLFTGF
                     ELGMMGFALFAAGFGADQLPALGVLALGHEVFIWFIFVSLLRATGERKRTPSALLREL
                     ATSPTIIAILAGLAANFAGFGGVLSTGVVGGTLTTTLNYLAAVVVPLVLLIVGYGTRL
                     TWRGVRQALPLVATRLPLATALALLLGRFVLSDWWGLDPIYAKALFTLLVLPPPFIVP
                     LFIPAERAEDAEYANNVLSLYSVASVAAFVTYLVLAG"
BASE COUNT         3321 a         7082 c         6778 g         3337 t
ORIGIN      
        1 tccactctct ttgcgcgatg actcgctaat gtcgtgtcat ggacctaccg gacggctacc
       61 tggacgccct cagcgacgag ttggcgccct acggcttcga attcggagcg accacccccg
      121 atgaggacgg cggcttggcg ctgcgctacc aggccgaccc tgagtcattc gctcgcgagt
      181 tcccgcaggc aggcgtcgag gattcctacg gcgtccattg gccgcctccc cacctcgccc
      241 tctggctacg gttcgaccgc cacggcgacc cgtacgaaat ctcgttcgag accttcgacc
      301 tgctggccga caccgcgtcc aatgatccgc agctcaacgc ccggctgaac acgctcgaag
      361 atccgtccga ccaggcgagc gctatcggtg aggcgctcta cgccgcgctg catccccagc
      421 accacgagta ctccgactac ctggagtgat ccgggtccag tctgactggc tgatcgctac
      481 cagaccagcg tcagccgatg cgatcggttt cggctgccca gaccgcagcc gcgtcctggt
      541 tgcttgtctg ccagtacttc caggccacgt agccggcacc ggcgagtacc agaacagcgc
      601 gtagtacctt cttcaagact cctccagctc tacttggtcg gctctgcgga cgcctgggtg
      661 gctaccgcca gcgacgggtt cgccagtgcg aagtcgtcgg tggcaaacgc ttggcgcagt
      721 gcgtccatcg agcgttcctc ggccaggact gcacgggcgc cactgcgggt caccgctacc
      781 tgaatggtgg cgatctcgtc cgcgtgcacc ttcgactcca ccaggatggc ctggatctcg
      841 tccgtggtca ggctctcgtc gagcttcagg cacggggtca acgcgttcag cacccgatcg
      901 aacctggtgg gctcggtgat cgccgagccg gcggacgccc gcacgagggt ggcacgtagg
      961 atctccgcgg acgcgagcga gcggtcgtgc gccggaatgc ccgacaggcg ttcgacggcc
     1021 tgggcgccgc tgacgaggtc accgtccagt tcgatcccgc ccaacgcgtc caccacatgg
     1081 gcgaaccggt cgcggtcgat ctgcaccagg tgatccatcc gcgccccagt cattcgctcg
     1141 agttcgcgca tcaccagcag cggatcgcga cggttggtcg acgccagtga gccggacgag
     1201 ccctgcagat cgatcgggaa cgcgatcagc gtaagttgct gccgcgacgc cgagaggtgg
     1261 gccaccacca gcgcatcgag agcgtccgcg gacgtgctgg tgagtacgta gttcaccgcg
     1321 ttgacgccgt cgacgaccac tgcggacggc cgtccggaat acgagccgag tgagccgatc
     1381 cgatcaatct cggacgaggc atcggagacg cggttcaggt agacggtgag cagcgccacc
     1441 gcaccaacgg acaccgcggt ggcgccgatg agcagcacga tcgcgagccg gtccagccaa
     1501 ctcctaccca cggcgcgcgc ctgcgtaccc tccgtcatcc cacctcctgt ccgcgccaag
     1561 cctaggcggg tgaggcaacc atgcgccgcc gactccaggc agcgctgcca ccggttagcc
     1621 tttccccatg cctgcggaga accagcgaca ggcggacgca cggcgccgcc acgaactcgc
     1681 cgccgcggct gttgccgctg aggcggccgc agcccaggtc caattggacg cgttcgtggc
     1741 gcggctgacg gccgcgtcca tcgatccaga gccgttgcag gccacgctgc tgaacggctc
     1801 cagggtgaaa accgacaagg tcggttggta tctcaacgcc gcccagacac tcgcgatcgg
     1861 cccggacggc acctactacc aactggtcac atcgggaggc ccgctggcca ggttgagcgg
     1921 cgtgcggatc ggtcccggga cgccaaccct ggttatcgga cgtggcggcc gtgacggcga
     1981 gaccggcgat ctcgcggact tcatcgaccg cgcttgggct cgctacgcgg ccgagtgagc
     2041 tcactaccga ccccagagac ggcaccaggg ggccgctgga tatccgagca gctccctggg
     2101 cgtctagggg gcgacctctg cgggtcgcgt gttgtattga ttcagtacac agcgtagagc
     2161 gctttcaccg gtcgtttcca ccaacggcaa gcgttgttgt gtaatcgtta acattggaga
     2221 tcatcctcct gtcctaagcg aagtttttgg ctgtccgaga ttgacaacaa ctgggcgggt
     2281 tcgggcaaca ccccggacaa cacccgttcg cgaagctggc cggcatggcg gtgagtggag
     2341 ttctgggagc gctggaagcg gagtggagcc gcttcgcgga cgggtcctca acccgggtat
     2401 tgcggtgggc ggagcttgac cgggcactgc tcggctgccg tggggtggac gccgtcctgg
     2461 ctcgcatccg gcgcgagccc gatgccgtcc tggccgcgct gctacgcatc ggacagggtg
     2521 gtgaccagtt ggccctgctg gtggtggtga aggcgatgct gggaaagttg gtgctgctga
     2581 gcgccggtcg acccgatgtg ctgatcgagg cgatctccga gatgtggctg gcgacggcgg
     2641 agtacccggt cgcccgccgg ccccgttcga tcgcagccaa tctcagttgg acggtgagcc
     2701 gtcggattcg agcatcgcca ccacgcacgt ccgcgccgct cacggtcgaa ccagcggcag
     2761 cgacgccaga tccggacgcg agcacgactc ttgcggacgc acgtcgattg ggcctgatcg
     2821 acgagctcac ccatcgcacg ctctgggcgg tgtacgtagc cggcctgacc agctatcagg
     2881 ccgccgcggt gctggacacc acacccgacc tggtcaggtg gcgctgcagt cgggcgatca
     2941 ggcggctccg cgagcaagcg gaactactgg cgtcctgact cggctggtcg agtctgtcga
     3001 ggccccgctg gtcgaacctg cccagaccgc gactggtcga gcttggcctg ccgcgagcct
     3061 gtagtcgaac ctcgggcggt cggggctgcg gacctgcttg gtaacgtgca ggccatggct
     3121 accgctttgg tcaccggcgc caccgccggc atcggcgctg cctttgctcg ggcgctcgcc
     3181 gaacgcgggc acgacctggt gctggtcgcc cgcaaccggg agcggctggc agcgaaagcc
     3241 gccgagcttg aggcacttgg cgtccgtgtc gaggtgttgc ccgccgaact ggccgaccgt
     3301 gcgcaagtgg cgacagtggc cgcgcgactg gagcaggcgg agcagccgat cgagatactg
     3361 gtcaacaacg ccggcttcgg gctcaaggca aagctgctgg atgtggacgt ccgcgaacac
     3421 gaccaggcct tcgatgtgat gtgccgggca gtgctcgtgc tcggcggtgc ggctggccgc
     3481 gcgatgagcg tccgcggtca cggccagata atcaacgtcg cctcgctgtc cgcctggatc
     3541 acgcagggca actattcggc ggtcaaggca tgggtgaagg tctactccga gagcttggcc
     3601 aacgaactgc acggaaccgg ggtgaccgta accgcactat gtcccgggtg ggtgcgcacc
     3661 gagttccatg accgagccgg cattcgcacc ggcagcatcc cggactggat ctgggtggat
     3721 gccgatcgcg tggctcggga agcactcgat cacgctgcgc gcggccgagt gctctcagta
     3781 ccgaccaagc gctggcaggt ggcccgcttc gccatgcagt tggcgccgcg tccgctggtg
     3841 cgcgctgcgt ccagaatgct gaccaagagc cgtgggcagt aacggatcgt ctcggattgc
     3901 gcgacaatgg cgaggaatcg aagagaggat cgccagtggc tgattcgggt accggcagct
     3961 attccgcggc ggcaccggcg gctgcgcgac tggtggcgca gcacctggtg atgaagccgt
     4021 atatctggtc tgcgctctca gtgcacaccc acggcatgcg caacctggag acgctgaagc
     4081 cgccgttcat cgtgatcgcg aaccattcca gccatctgga cgcgcccctg atctatggcg
     4141 cgctgccgcg gcggctgtcg cgcaatctgg ccgcggcgac cgcgggcgac tacttcttca
     4201 acgcctggta caaggcgctg ccgaccaagc tcttcttcaa ctcgttcccg gtcgcgcggc
     4261 aggggcacgc gaaccgggcc gggcagcggg gtctggctgc gcacctggtg aatgacggcg
     4321 tcccgatcct gatctttccc gagggcacgc gctcgcggac gggtgcgatg gccaacttca
     4381 ccccgggggc ggccgcgctg agcatttccc ggaatgtgcc ggtactgccg atcgccctgg
     4441 tcggtgctta cgcggcgatg ccgtacgggg ccaacgtccc ggttcccgga cgtccgcacg
     4501 tccacgtggt gatcggacgg ccgttgcgcg cggcgcccag cgagatcgcg caccagttct
     4561 cggaacggtt gcaccgctac gtggtggaga tgcatgacac caccgctcgg gcctacggca
     4621 tgcccaccca ggccgatttc gcgcgcgcgg tcgcattgcg gaccgccgcg gcccaggccg
     4681 acgaggccca gaaggccccg gtgatcgagc tcagccgtcc caacgatgcg cagtccgcgg
     4741 tcgttgagcc ggttccggaa ccgtcccccg aaaccgagcc caccaccgag gacccgccgg
     4801 ccaaagccgg ctgatccagn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt
     4921 cggtcacgat cgcgcggatc gcgtccagcg ccggagtcaa cggcgagaac ggacgcaagg
     4981 cctcgaacaa ccccggcgcc gcggacgtaa gcgcgtcggc cgtagtcagc accgcaaagc
     5041 cgattgcgac caggcgtccg acgccgccga accaggaaac cagcgcatgg ttcagcacta
     5101 cgaacgtcac cccggccagc aacagcaccg ccgtgatcgc cagccacttc tgcggcgaaa
     5161 cgttcaatcc gaactgcgcg aggcccgaca ccacgagtgc ctggatgccc acaactacga
     5221 cacccggcag cagcgaccgt ccgatcagca ccgccgtcgg ctcggacgaa ctcagcagcc
     5281 cacccgcgat tgccttcgtc accgcgtaca ccgcgagcgc gcccagccac agcgccagca
     5341 ccagcaccag gctgatccac ccgacgttgg cgttcgccaa cccggccagg tcgtcggtct
     5401 gcaccggcga agccaccaca gtggccagcg tctcccgatc ggattccgta tagctgggga
     5461 gcttgtcctt gccctcggcg atgccgtccg acatctcccg catgccgtcg gccagcttcg
     5521 tgccgccgtc cgccgcgctg atcaggccgg cgctcaactc gtcgacgccg tcggccagct
     5581 gcccggcccc gtccgctgac tgaccgatcc cgtccaccag cgccggtagc ccgtccgcga
     5641 gttcgtccgc accgtcggcg agcttgccga tgccgtccac cagcgcgggc ataccgtccg
     5701 ccaactgatc ggcgccgccg gccaactggc tcaccccgga cgcgagcgcg ggcagccccg
     5761 ccgccatcgt ctgcaggccc tggtcgagct gggtgccgcc ggccttcagc tgagccaatt
     5821 gcttcttctg tgcagccact tcgtcagggc tcggcacacc cgagagctga ccggcgatct
     5881 ggcccagccc gtcgtcgagt ccaccggcca catcgagcag gctgaccccg ttctgcttgg
     5941 cttccaaccc tgcggcggcc gcctttccgc cagccgctac cccggcacca gcaccgacct
     6001 gcatcgcgtt cctgaccagg tcctcgcagc caccatcgcc gccgcacgcc gcgagcagcc
     6061 ccttgccctc gctactagcc aaggcgccct ggtaggcgcc tgcgaccaga tcgccgttcg
     6121 agccgagctt ctccagctgc gactggtagg ccttgaggcc accgttcagt ccgctggccg
     6181 cctggctcaa ctgcttgatg ccgccggcca gcgccacctg cccgtcgagg gagttgactg
     6241 tctggtcgat cagcgtgctc acgcccgcca cgtagctggt cagcccgctg gacagttcct
     6301 tgctgccagc cggcagatcc ttggtgccgg tctcaagctg ggccagcccg tccgccaact
     6361 gccgtgtacc ttcgggcagt tccttggtct tcttgtgcag ggtgcccagt ccgtcggcca
     6421 gttgccgggt gcccgagggc aggtccgcgg tcttggtgtg catggtgtcc agaccgttgg
     6481 cgagcgcacg ggcgccggtg tagagctgct tcgacccgtc cgcggcctca ccgatgccgt
     6541 ccgcgagctt ctcggccccg tccgccagct cggtggcccc gtccgccatc gtcacgaact
     6601 gctcgcccat gtcgttgaag ccgatgtaga tctggtcgag atacgactcg gtgagcatct
     6661 ggttcagcat cttcgcggcc tcagcggcca ccagcttgcc cagcgtcgcg tccgccaccc
     6721 cggcgaccgg cgaagtgtgc acctggatcg tcgcctgctt ggcctggtcc ccctcgttct
     6781 cggcgaagga cgtggccgca gccgagaagt tctccgggat caccaccatc gcggcgaact
     6841 caccggtggt cagtccagcg cgggcgtcgg cctcgtcggc gagcacccag gtgaggttct
     6901 cctggcgatc ggaatcgacc aggttcgcgg tcaactggcg gcccagtggg gtgtactggc
     6961 cgtccagctc gaccggctcg tcaagattca ccaccgcagc ctgcaccgtg tgcaagcggc
     7021 tgtccgagtt cacccctgcg aagaggaatc cgcccgcgac cagcagtggg accaggatca
     7081 gccccaacag cgcgtaccac ttcgccggcg tggaattcac gagttcggcc ttcatttcgc
     7141 tccttccagc gcgaggtggg aggtcagctt cagcaccctg aacggcaccg gcagctgcgc
     7201 ctcaagatcg gatcccggca tcacgccgag aacccaagtt gtcggctctt cggccttggc
     7261 ggacgcggcc agcgcggcca gcagcgcggc gtcccgggag ctggagagct cgtcggcact
     7321 gtccacgcac accaggccgc cggcctgctt ggtgagcacc ttcttgaagt ccgggacggt
     7381 cggcccgagc accggcgcct ggcggcgcag caccggcgac tcctccggaa gcacataccc
     7441 gagcaccttc agcttgcctt cggtgagctt cacccgtccg gcgatggcca gcagcagggc
     7501 gcgacgttgg gcgtgctcgc cctcgatcac cagcacctcg ccgggctgga cgctgagcgc
     7561 gaggtcggtg aacagcgtcc gccgatcggt gcgggcggtc agcccctcgc cgacgatcgc
     7621 ggatgtgtcg tcgggcgacg gccactgggc caggtgcacc tgccgggtga gcgcttcgcc
     7681 ctcgatgtcg agcgcgggca accgccggtc gagccacttc ggcagccacc acgcgtgccg
     7741 tccgagcagt ttcatcaccg ccggcaccag ggtcatgcgg acgacgaacg cgtccagcgc
     7801 gacgcccacg gccaggccga acgcgatcgg cttgatcgca ccctcgccgg cgggaacgaa
     7861 gaacgcgaac accgagaaca tgatcagcgc cgcagccacg accaccttcg acgagtgcac
     7921 gaagccgtcc tcgaccgagc gttcgacgtt gccatggacg tattcctcac gcatccggga
     7981 caccaggaac acctcgtagt ccatggccag gccgaacagg atgcccatca ggatgatcgg
     8041 caggaagctg atcaccggac cggcttccgg gaggttgatc aactgcttga accagccctg
     8101 gttgaagacc agtgtggtcg cgccgaaggc cgccgcgacg ctcagcaggt agcccagcgc
     8161 ggccttgatc ggcacccaga tcgaccggaa caccagggcc agcagcacca gcgacaagcc
     8221 gaccacgaag acgccgaacg gcaccagggc ggcactcagc cggttggtga cgtcgatctg
     8281 gatcgcggtg aagccggtga tctcggtgtt cacgtcgtag cgctgctgcc attcggcctg
     8341 gcgttcccgc aactgctcga ccagcgccgc ggtcgccgga tcgtccgggc cggtggtcgg
     8401 gatgatctgg accatgccgg tgtcgacgtt cgcgttcggt gtggccgcgg cgaccatctt
     8461 cacgccgggc atggcctcga tttcggcctt gagcccgtcc agcacctcaa ccgggtcatc
     8521 agactcgacg atgccgacga tcatgatcag cggaccgttg aaaccgaccc cgaaggtctc
     8581 ggtgatcgcg tcgtaggtct ggcggtcctg ggtgccgggc agcgagcgtc cggacgtggg
     8641 cagggccatc tgcagatcct tggccgggta agccagcccc ccgagcgcca ccaggaccac
     8701 tgcgatggtg accagcggcc acttggtgac cacgttcacc caccaggtgg acgcgctgaa
     8761 cttgcgcttg attgggggtt cggtcgcacc tgagttcagg ggaacagccc ctgagcctga
     8821 aggaacgacc cctgagcttg acgaaggggg ttggtcaagc gcagcccccg tttccgacgc
     8881 ttcgccctgc acaccccgac tcccccgagt cttggatttg ggacgcaacc gctcgccggc
     8941 gaagcccatg aacgccggca gcagggtcaa cgcgagcacg acctcgaacg cgaccgtgag
     9001 cgagccgaaa acgcccatca cggtcaggaa cggcaggccg gcgagactca acccgatcag
     9061 agcgatgatc acggtcaggc cggcgaagac caccgctgaa ccggccgtgc cgaccgcccg
     9121 cgccgcggat tcctccacgt ccataccggt ggcgagctga tcccggtgcc gggagatgat
     9181 gaacagcgcg tagtcgatgc ccaccgcgag cccgagcatc accgccagga tcaaagtggt
     9241 cgagctgatc gtgatcacac cggacgcgat ctgcaccgcc agcaccccga gcccgacgcc
     9301 ggcgatcgct gtcccgaccg gcatcattga ggccaccacc gagccgagcg tggcgatcag
     9361 cacgatcacc gcaacgccca gaccgagggc ctccaccgcg ctgatcttgg gcacgtggat
     9421 cgagaagacc tcgccaccca tcagcacttt cgagccgggc agggtcttct caagcgtctg
     9481 gccagtctcg gtgagctcct cgcgctgcgc gtccgtgatc gtggaagcct ggccctccac
     9541 gcgcacattg gcccggccgc tggtgccgtc ctcgctgatc agcccgtcga cgtgctcgct
     9601 gaacggtccg atcacgccgc ggacgtaggg cagcgcgtcc atgcggtcgg tccagtcctc
     9661 gatcgcggcc gtcacgtcgg cgtccgtcag cttcttgcca tcgggcgcgg tgatcaggac
     9721 ggtcgcggac gcgtccgctg cctcggggaa agtgaccttg agctgagcga gtgccttggt
     9781 cgagcttgcc ccgggcacct cgaagacgtc atcgaagtcg ccaccgacgg ccagcgcgag
     9841 gccaccgaag agcaccagga tgatcgccca cacgccgacc acgcgtccgc gcaggcggta
     9901 gcaggctcgt ccgattcgat agaggtaagc ggacacgtgc gcccttccgg ctccggtaat
     9961 acgttccgtt tcgctgtcgc gagcggttcg atacagtagt gtattcaata caggtccgta
    10021 ttgaaaaggg cggaggccac cgtggacaac caccccaccc tcaccccgcg acgcgtccag
    10081 acccgcgaga agctgatgga cgcggcggtt tccctcttcg cagagaaggg tgtgctgggc
    10141 gccagtgtcg aggagatctg cgagcgtgcc gagttcaccc gcggcgcctt ctactcgaac
    10201 ttcgccagca aggacgagct ctgtcttgcg gtgatgaacc ggcaggccga acgcgacctg
    10261 gccgcagccg cgcgaacggt cgccgggctg gtggacgaca tgaccgcgcc cacgtcgctg
    10321 gacgaactga tcgaacgagc cgtcggtgtc tttctggcca cgcagccgcg ggatcccgaa
    10381 gacctggtgg tctcgatgga gctgcggctg tacggcgtcc gcaaccccaa gctgcgcgaa
    10441 ccgctgctgg cgctcaccga cgaagtcgcg gccgagctgg gtggcatcat ggcccaggca
    10501 ttgactcgcg agcgcgcccg gatgaccgtc ccgatgagcc aggcgatcga cttgctgcac
    10561 gccgtctacg tgcacagcgg catcaccgcg ctgttgaacg gactggaacc cgacgacgag
    10621 tcgcgccggc ggcagctgac ggtgttgctg cgctcgctgg tgaccgtcga ctgatccggg
    10681 caagatcaaa ttgggttcga tccggcgctg accggctggc tgcggccgga taggctcaag
    10741 ccatgaccgc tacggccaac gaataccctg acctgaactc ctccaagctg gcgatcgtgg
    10801 gtgccggctc cgtcggcacg tcgatcgcgt acgcgtcaat gatccgcggt tcggcgcggc
    10861 agatcgcgct ctacgacatc aacaccaaga aggtcgaggc cgaggcagca gacctggccc
    10921 acggcaccca gtacacgccc acctcccagg tcaccggcgg ctccgacatc gaggtggtca
    10981 aggactcatc gatcgtcatc atcaccgcgg gcgcccggca gcatccagga cagacccgca
    11041 tggacctggc tgcagagaac accaggatcc tgtccgaact gatgccacga cttctggatc
    11101 aggcgccgaa tgccatctac gtgctggtaa ccaatccctg tgacgtgctc accgtggtgg
    11161 cgcagaaggt gtccgggctg ccgcccaatc gggtcttctc gaccggcacg atgcttgaca
    11221 ccgcccgggt ccgctgggcc attgccaagc gcgcgcgggt ggcgcagtcg aacgtgcacg
    11281 cggtgatcat gggggagcac ggcgacaccg agttcgcgat ctggtcgagc gcgtccatcg
    11341 cccaggtgcc catccgcaag taccgcgacg aggccggcca ccgtgtgttc agtgaagaga
    11401 tcctggctga gatcgcgcac gacaccaccc acgccgccta ctcgatcatc gagggcaagg
    11461 gtgcgacgaa ctacgcgatc ggcctgaccg gcgcccggct gaccgaagcg atcctcaagc
    11521 agcagcgcaa gatcctgccg ctctccagtg tgctgaccga ctaccgcggc gtgtccgacg
    11581 tcgccttgtc ggtgccgagc atggtgaccg agaagggaat cgagcgcgtc ttcgaggtgc
    11641 cgatggacgc tcatgagatc gccctgttcc aaaactccgc ccggcacgtg caggcaacct
    11701 gccgggagtt gggcttctga cccgctgact gcttgttctg gcgcggatcg cgtggatcgc
    11761 gttcccggct agttggcttc gggcggctga acccgagttc gatggcattt ggggcgggtt
    11821 ggcccgactg atccaggcaa aacccggttt tcgatggctt atcgggctgc tggcgagggt
    11881 cgataagcca tccagctcgg gtcaggctca cccgcgacga cgacagggcg ctggtatgcc
    11941 atccaagtcg gcattggctt cgtcgcaccg cttgaggcgt tgcggcgcga cgcctcagac
    12001 cggacgggac gcaaacagcc cgagtccgag ctcagatgcc agccgggccg cagcttgcac
    12061 cccgcggacg ctgttgccgg cctcgtctag gcgcggcgag aacgaaccca ggccaccttt
    12121 gccgggagac accgtgacga tgccaccgcc gatgcccgac ttgccgggca gtccgacgtc
    12181 gtagagccac tcccccgaac gctcgtacat gcccgcggtc gccatcaccg ccaacacgtg
    12241 gtggcatagg gcggacggga tcacttgcgc accggtgacc gggttgcgtc cgccgtcggc
    12301 aagagtcgcg cccatcatgg cgaggtcgtg cgctgtgacc agcaggctgc tctgccggct
    12361 gtagagctcc agcgcaacct caggggcgga cgcgagcgca ccctggccat gcaacgcggc
    12421 ggtgatctcg cgattgcgga agttggtcgc gctcacgctt tcgtagaggg ctgagtcgat
    12481 cgccaactcg cgcccggcga atgcggacag gccggtcaat aggtgcgccc aacggccctc
    12541 tagtccgttg cccgggaggc ggctgcaggt ggcgatcgcg ccggcgttga ccatggggtt
    12601 cgtgcgtccg ccggaagcgg ccaacaccgg gtcgaccgcg ttgaacgcct gcccggtggc
    12661 attcacgcca agttcgtcgc gggcccgctg cgcaccgatg tgcgcgcaga ccagggcgaa
    12721 ggtgaacggc ttggcgacgc tcatcatggc gaagcccgcg aaggcgtctc cttcatcgaa
    12781 gacccggccg tcgctggcca ctacgcacag cccgaacttc tccgggtccg cgtccgccaa
    12841 cgctgggtag accggggagt tcgcgcccgt ccggttgttg agcacgtctg cgtacgtcgc
    12901 ggcgagcgca cgccgcacgt ccgggctggg cgccaggcgt ccggtggaaa cgaagtcgtc
    12961 gctcacgccg cgaccttagc ttgaacagaa tggggacggt tccttttccg ttcacttttt
    13021 ccacagacca atcgctcaca gccaggacac ctgtggataa ctggagggaa aaggaaccgt
    13081 cccctttgcg ttcagtaggg ttcgggcatg actcacgtcc tggtgaccgg cggggtgcgt
    13141 agcggcaagt cgagctacgc cgaggctctg cttgacgcgg acgcggccgt cacctacctc
    13201 acccccggct atcccgcgga cgccgtggac gccgagtggg ccgcccgggt ggccgctcac
    13261 cagctgcgcc gtccgcctgc ctggcggacg atcgagtcgc tcaacctcgc cgagacgctc
    13321 cgcgcagtcg acggcccagt gctgatcgac tgtcttggca cctggctgac cagactcttc
    13381 gatgcctggg acgcgtggga ggacgactcc gtccatcacc tcggccgttt ggacggcgag
    13441 ctgtccacgc tcgctgcagc cgtgcgcgaa cacacgtccg atgtgattgt ggtgaccaat
    13501 gaggtgggct ggggcgtggt gtctgcgtac aagtccggac gggttttcgc cgatcagctt
    13561 ggccgggtga accaggccat cggcgcagct tgcgaccagg ttgtgctgat ggtggccgga
    13621 cgagccctga tgcttcctga cggaagcggc ggcccttcct gacagtcgcg ggtccgcaag
    13681 gctctcagcc cggttcgtcg tcaggcccgg atcatccaga ccactagcag caccgtcgcg
    13741 gccacctcga cgatggcgcc gagaacgtcg ccggtcacac cgccgaagcg tttcttgctg
    13801 cgcagcagca gccaggtgca cgccaccact gccccgatca gggcgatgcc cggcagccac
    13861 caagcgcggc cgtcgacgca ggcaagtccc tgcaacgccg ctagccagac cgccagcgcc
    13921 acaaccgctg cagccgccgg gatgctttcg gccaccagcg cacccagccc gcccggacgc
    13981 gctgccggca gcccgacgcc gcagcccagc agcagcgcga accgcgacac cgccaggagc
    14041 accgcgacca ccagccaccc gtagggccgt tccagcagcg caccgcttgc gatcacctga
    14101 gccagcagca ccagcaccag agcgaccgtc cccatcggac ccacgtcgcc gcgcttcata
    14161 atctccagcg caccggacgc gtccctgccg ctgcccagtc cgtccacagt gtcggcgagc
    14221 ccgtccaggt gcatcgcgcg cgtcccgaac gccatggcac ccaccgcggc cactccgcct
    14281 aacaggccgg gcagccccag agccaccgcc ccgaaaccca atccggacgc catcagcgtc
    14341 accggcagca ccgccagcgg cgccaccagc atcgcgaccc gcgcctgctc gcgtccgatg
    14401 gtttccggcg gacgcaccgg aacgatcgtc agcagcccca gcgccagccg aaacccagcg
    14461 ccgaggttca cgtcaggtca gccagctgcg cgatgccggt caacgcggcc accgccgacc
    14521 gcagcatcgg gacggccaac gcggcgccgc tgccctcgcc gagccgcatc gagaagtcga
    14581 ccagcggctt cagtccgagc cgatccaagc ccaaagcctg gcctggttcg gtggaacgat
    14641 gcccggcgcg cagccaggct ttggtcccgg gggcgaggtc ctcggcaacc agggcttcag
    14701 ccacggagat caccccgtcc agcagcaccg ggacgccacg acgagccgcg ccggtgatga
    14761 aacccacggc agcggcgaag tcggccgcac ccagtgcgat cagccgctcg actggatcgg
    14821 acgcccgcgc gccaacccgg tccagcgcgg cctgcaccaa ggcggtcttg tgcaccagtg
    14881 cggactcgtc gatgccggtc ccgcggccgg tcaccgcggc cgcgggcacg cccagtgtgg
    14941 ctgcgatcag tgccgccgcg atggtcgtgt tgccgatgcc gaggtcgccg gcgatcagca
    15001 gctgggcacc gtccgcggtc tgctcggctg cgatctggtc gccgaaatcc agcgcctgct
    15061 cgacctcgtc ccgggtcagg gcgtccgcca ggtgaatggc gccggaagac cgccgcacct
    15121 tgaaccggct gatctccccc ggcaggtcgg gaaagtcgac cgcaaccgcg atgtcataaa
    15181 tcgagacccg gacgccgttc gccgccgcca acgccgagac cccggcctga ccagtggcga
    15241 tgccgtacac catggctggc gtgatcgcgg cagggtaggc cgagacaccg tccgccgcga
    15301 tgccatgatc gccggcgaag acgaccaccc ggacatcgtc cagttgcctc ggtgggcacg
    15361 tcccctgcac ggacgcgatc caggcaccca gctcctcgag ttcgccgagc gcacccagcg
    15421 gcttggcctg gccgtcgctg agttgacgag cggcggcgta ggcgaactcg ctcgggacgg
    15481 tgatggtctt gctgggcaac atgggcgtct ccaaaatggg ctcgaaaggc caccctaggg
    15541 cgtgtccggg ttggacgacg ttggcagact ctgggtgggc gattcgacga ccgaaaggga
    15601 cgcagtgctg acgctgctgg ttgggctggc gattctggtc ggcctcgtgg ggatcgtgat
    15661 accggtgcta cccgggtcga tcctgatcgg gctggccgtg ctggtctggg cggtcatgac
    15721 cggcaccgtg ctgtcctggt gggtgttcgc cgcggtcgcg ctgctgctgg tcgtcggctc
    15781 ggtgatcacc tgggtcatca ccgcgcacca caccagggct gccggcgtgc ccaatgggtc
    15841 gctggtgttc gccggactgg ccggcatcgt gggcttcttc gtgatcccgg tgatcgggct
    15901 gctgctcttc tttccgcttg gtctcttcgc agccgaatac tggcggctac gcgatccggg
    15961 cgcagcccgc gcgtccgccc tggtggggct gaaggccacc gggctaggca tgttgaccga
    16021 gctcggactg gcgctggcgg ccgctgcggt ctggctggtc gctgtcctga acggcgtgtg
    16081 aggacgtccg cgcagggcta agttggagag cacacgcgac gtgcaaacga aggagatcgc
    16141 catggcttcg aagagccgat tcaccctgcc aggcctgtcc caggagaagg ccagcaaggt
    16201 ggtgaagatc ctgcaggagc gcctgaacag ctacaacgac ctgcaattga ccctgaagca
    16261 cgtgcattgg aacgtcgtcg ggccgagctt catcggcgtc cacgagatga tcgacccaca
    16321 ggtcgaccag gttcgcctgg acgccgacga ggtcgctgag cgcatcgccg ccctgggggc
    16381 atcgccgctg gggacggtcg gcgcgatcct gaccactcgt gactgggacg actacccgct
    16441 cgcccgcgac accgtgccgt cgcacctgac cgcgctcgac aaggagtacg accgggtgat
    16501 ctccgaaaac cggcaggcca tcgaggacgt cgaggacctc gacctggtca cccaggatct
    16561 gctgatcggc cagaccggtg ccctggagaa gttccagtgg ttcctgcgcg cccacctgga
    16621 gacctcgtcg ggcacgatcc ccaactgatc agcaacgatc cctgcggagc actggcgagc
    16681 ctgcgagcag cgcccgtttg gggaagacct cagtcgtcgg tgagcgcgat gtccaccagg
    16741 atctcggacg cgatccgctc cagttcggca cggatctcgc cgagatcggc accctcgggg
    16801 acgcgggcca cgacctgagc ctcgaacagg cgtccgccgg ccatcggcgc gtccatggtc
    16861 tggctggtga ggctctcgat actcagctgg tgacggctga gcgcggcgga gatgtcccgg
    16921 acgatgcctg ggtggtcgtt gccgagcacg ctcaacgaca gtccctgcca gtgtcccggc
    16981 gcgtccgcgg cggcaccggg gtaagcggtg accttcagca gcccgtccag ttcggcgagc
    17041 gcggaagtga aggcaaccgc ctgatcgtcg ggcaccgaga ccacgacgat gcccgcgaag
    17101 gtgccggcga gttcggcaag ctgactgtcc tcccagttac cgccgtgcgc ggacacaacc
    17161 tcagcgagcg cagcgaccag gccggcgcga tcatcgccga caacggtgag aactaggcgg
    17221 gacacgtccg catgctaccg gctccacctg aacgaatagg ggacggttcc ttttccgttc
    17281 cggttcgcgg gccgcagcat ggctactgtg atcgcatgaa ctcagagaag gtcgtgttcg
    17341 gcttcttcgt tctgctggcg gcgacgctga acttcggcta cgtgaccgga gagatcggca
    17401 acccgctgca ccacaacgcg attgagcttt acgcggcggt ggtggtgaac ctgatcgcca
    17461 ccgtgctgaa gctcggcgac cggacgcaga tgggcgcggt gcacctcgcg tccagcctgg
    17521 tggcgtccat ccagctgctg atcgccgccg gaatctggat ctgggagacc caggggctcg
    17581 gcgtcgatcc gctgcaggtc ggcgagcgag cgatgagcgg ggtggtgtcg ctcgcgctcg
    17641 gggcgctgct ggcgaacatc gtgtcggtgg tgctgatggt gatcgaaacg gccaacttcc
    17701 ggcggcgggc ctgaccatgg ccgaccgccc ggacgtccgg gccgaatcgg ctaccgaact
    17761 gcggcaagcc gagatcgcgc tgccggagac gtcggcgtcg acggacgcga tcttcattct
    17821 gatgcgacgg atgcgcagtc cgctggtggt gctgctgatc gtgttcgccg tggacgtgct
    17881 cggcctgtcc ctgatgcccg gcgtggatgc gtccggcgcg ccgcatcgca tgacgatctt
    17941 cgacgcgttc tacgtgatga gctatacggc gaccacgatc ggtttcgggg aaatcccgta
    18001 cgagttctcg atcgcgcaac gcatgtgggt caccttctcg atctacagct cggtggtggt
    18061 gtgggcgtat tcgctgggaa ccctgttcag cctgttccag gatcgcggct tccgcgaggc
    18121 cgtgcagctg cagcgggtcg ccggccaggt gcgacggctg cgggagccgt ttctgatcat
    18181 cgccgggtac ggccagatgg ggcagcggat cgcggagacg ctggacgcgc agggccgccg
    18241 cgtggtggtc gtcgactccg acgatcatgt acgcgatcag ctggccggcg gcaagctttc
    18301 gatcgacgtg ccggccatcg atggcgacat tcgcgatccg cggacgctga ccctggctgg
    18361 gctcggccac ccccattgcg cgggtgtgct ggcgatgacc cacgacgatt cggtgaatct
    18421 ggcagcggtg atggcggttc ggctgctgcg tccggagctg ccggtatacg cgcgggcgaa
    18481 cgatcgggtg acggccgcgt ccatgcacga tttcggcgcg gacggggtga tcaacccgtt
    18541 cgaccggttc ggggagtacc tgatcctgcg acttcagcgc ccggtgacgt atcggctggt
    18601 gaagtggctg ttggccgcgt acgactcacc gctggaggag tgccggacgc ggttgccctc
    18661 cggacgctgg atcgtggcgt ccgacgatca cttcggcgag gagatcgcgt ccgatcttcg
    18721 ccgggcgggc gtggtggtcg aggtggtgga cgcgcaggag tcgctgccca acctgcgtga
    18781 tgtggccggc ctggtggccg gtgcggcgga cgagaaccgc aatctcgcgc tggccggaag
    18841 tgcgcggatg tcgcatcccg gggtattcct ggcggtgcgg gcctgctcgg agggcctcgg
    18901 cccgctgctg gacgcgttca gtccggacat cgtgttcatc ccggcccggc tgaccgcgga
    18961 agaagcgctg gcacgggtgg tgacacccga cttctgggat ttcttccgat tcgcgttcaa
    19021 ccaggacgag gaatggtcgc gggaactgct ggaccggctg gtggcccggc agggcaccgg
    19081 gtcgccgacg agccggcgga tcgtgctgga cgagacccac gcgcccggcg tgttgaagtg
    19141 gttgacgcgg cattcgctca accttggcga cctgctgcgt gacccggacg accgcgagca
    19201 gccgttggcg ctggtgccgc tgatcgtcac ccgtgagtgc gtcgacacct tcgcaccggc
    19261 cgacgacttc gcgttggcgc ccggcgatga actcgtggtg gccggtcacg acgccgcgat
    19321 ggacgtgctt gaccggaccc tgttccaccc ggacgccgtc gagtacgtgg ccaccggaaa
    19381 tgtggtgccc gccacttgga tcttccgcaa gctcagcccc cgcgtccagg acgcctagcc
    19441 cagggacgcg ctggtccccg tcccgacaac cactactcgt tcggtgctat tcgggctgtg
    19501 acgcgcaccg gacgggaccg accaccggct cggccacagg gtgaagaagg accgcagtcc
    19561 gaggcgctgc agccagtaca cgcaccatcg tccgaatccc gactggcgtc gacagtcggc
    19621 acgaccggcc tgccagactg cggccatgtc tttggttgaa tcgcgatgaa caccgacccg
    19681 agcgcactga tcggctcggt cgcgccgatt ctcgtcctga tcgttctcgg cgcgctgctc
    19741 gcccgaacct ccgtgctgga cgacgccaca gtcgccggtc tgaagcggat catcgtcaac
    19801 gtcgcgctgc ccgcggtgct gttcaccacc ttcctcaaaa cccggttcga gcccgagcac
    19861 ctctggctgg tgctgctcgt cttcggcctt tgcgtcgccc tactcactct cggccgggtg
    19921 ctacccgtcg gtccgcccag ccgctaccgg ccgttcctgt tcaccggttt cgagctgggc
    19981 atgatgggct tcgccctgtt cgcggccgga ttcggcgccg accagttgcc ggcgctcggc
    20041 gtcctggcgc tgggccacga ggtgttcatc tggttcatct tcgtcagctt gctgcgcgct
    20101 accggcgagc ggaagcggac tccgtccgcc ctgctccggg agttggccac ctcgcccacg
    20161 atcatcgcga tcctggccgg cttggcggcc aacttcgccg gtttcggcgg cgtgctcagc
    20221 accggggtgg tgggaggcac cctgacgacc actttgaatt acctggccgc ggtggtggtg
    20281 ccgctggtgc tgctgatcgt gggctacgga acccggctca cctggcgcgg agtgcgccaa
    20341 gccctgccgc tggttgcgac tcggctaccg ctggcgaccg ccctggcgtt gctgctcgga
    20401 cgcttcgtcc tgagcgactg gtggggtttg gacccgatct acgccaaggc gctgttcacg
    20461 ctgctggtgc tgccgccgcc gttcatcgtt ccgctgttca tcccggccga gcgcgccgag
    20521 gacgccgagt acgccaacaa cgtgttgtcg ctgtactcag tggcttcggt cgcggcgttc
    20581 gtcacctatc tggtgctcgc cggctgaact tggcggac
//
LOCUS       DAOPRQ010000045        20332 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_2230_length_20332_cov_12.135128, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000045 DAOPRQ010000000
VERSION     DAOPRQ010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 20332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20332
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2230_length_20332_cov_12.135128"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            107..180
                     /locus_tag="PKJ61_07280"
     tRNA            107..180
                     /locus_tag="PKJ61_07280"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:141..143,aa:Val,seq:tac)
     gene            240..566
                     /locus_tag="PKJ61_07285"
     CDS             240..566
                     /locus_tag="PKJ61_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13665.1"
                     /translation="MSAHLPSAGRARWRPWWAIAVAIATLGAGALVLWWGVAAIATDR
                     PNQWFSGFGTVLGTAAIVAALPPMACLTVFLARDTRGWFWAGTGLALTVAAVVISVVI
                     GDSNFR"
     gene            642..2240
                     /locus_tag="PKJ61_07290"
     CDS             642..2240
                     /locus_tag="PKJ61_07290"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOC13666.1"
                     /translation="MVGYLGLGERLGAYRVDAELGRGGMGTVYRATHLPLNRPVALKI
                     LDPQLAADPVFVERFHREAAILASLDSPHIIRIFDHGEIDGHLYLATQYVAGGDLHEL
                     VRGSGPLRPETALQVYGQILSAVGDAHKRGIVHRDVKPSNVLLHPDVVEPFAYLCDFG
                     IAQSKEAGLTEAGAVAGSWAYMSPERQTGSPATPQSDIYSAACVLWFMLTGRGVYTGT
                     DVEVAMAHLHSAVPQLRGVDPLLVELNRLFRTCLAKDPAERPATAAEALALSRDCQPL
                     AVGTIPLDLNSPDSGIFRTTLKPGDPGPLADPARRSGRRVGLLVAAGVAALAVGATAT
                     MLVLPTQARSALTAVAQPGASPSGPSASSASDPPAPGYLCWDAQPAADLSACTNPSGR
                     SGLRYLYPSLGEQWADCKYVAYRSTTDAYDCTIKHLGLIRYRYWTDVAEANQHYSQKY
                     AKATETDLVLDGNVVGTLYRLPRPVAGEYRMSGFWLDGHFSFSVEAPTKAQREQLFKL
                     VQLRGYDQLTGKRKGADPTIGVLS"
     gene            complement(2233..3075)
                     /locus_tag="PKJ61_07295"
     CDS             complement(2233..3075)
                     /locus_tag="PKJ61_07295"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF024744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HOC13667.1"
                     /translation="MGLAGSGCAAVPVVSDYSASWTQSAGQVASASVATLPIKSVTNF
                     RDVAGPGLALPGGRTMTIGVVYRSGKLATLSASDRAYLVAAGITDIVDLRTPAVAKAA
                     PDRKIPGTKRHSANVYAVYRTPAPPTRSVAAAKAHMQKVNTDFVAKSAQRKQIAKALK
                     IVAAAKGPVIIHCTEGKDRTGWLAALLQLIAGADREQVLAEYLKSNEYREAAIAKAYS
                     ATLFRQGRTAAKAKRALLTVEPAYLNAALTRMDRQYGGLDGYLGKGLGLSAETIAQLK
                     AKLS"
     gene            complement(3113..3538)
                     /locus_tag="PKJ61_07300"
     CDS             complement(3113..3538)
                     /locus_tag="PKJ61_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3052 domain-containing protein"
                     /protein_id="HOC13668.1"
                     /translation="MSDAPGATTRAPIVAALGIAKGQAVQELGWDEDADEGLRSALLD
                     ELGEDFVYEAIEAVDVVVLWWRDEDGDLADGLVDALTDLTERGHIWLLTPRVGRDGYV
                     DPTDLAEAALTAGLALANTASVSRNWQAQKLVRPKGGRR"
     gene            complement(3620..6382)
                     /gene="aceE"
                     /locus_tag="PKJ61_07305"
     CDS             complement(3620..6382)
                     /gene="aceE"
                     /locus_tag="PKJ61_07305"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HOC13669.1"
                     /translation="MASRNSVGPILNGLPTNLPDIDSDETAEWLESLDQMIEQDGRNR
                     ARYVMLRLLERARNQNLGLPSLTATDYVNTIPPEREPWYPGDVELERKFRRLARWNAA
                     IMVHRAQRPGVGVGGHISSYASSATLYEVGFNHFFRGQDHPGGGDQVFFQGHASPGMY
                     ARAFLEGRLDENDLNGFRQEKSHIVDGRVRALPSYPHPRQMPDFWQFPTVSMGLGPIA
                     AIYQAQFNKYLHNRGLKDTAQQRVWAFLGDGEMDEVESRGALQLAGYEGLDNLTFVVN
                     CNLQRLDGPVRGNGKIMQELESFFRGAGWNVIKVVWGRGWDPLLATDRDGALVNVMNS
                     FTDGDYQTFKANDGAYVRDNFFGRDPRTAKMVENWSDDRIWKLTRGGHDYHKVYAAYQ
                     AATQFVGAPTVILAHTIKGYFLGSHFAGRNATHQMKKLALDDLKQFRDRLEMPITDAV
                     LEENPYQPPYVNPGPGDERIQYVLERRRALGGPVPQRRYQPQPLKLPDEAAYAGVKKG
                     SGNQAVATTMAFVRLLKDLMRDKEFAPHVVPIIPDEARTFGMDSFFPTIKIYSPHGQN
                     YTPVDHELMLAYREARTGQILHTGINEAGSVAAFQAVGSAYSTHGLQMVPIYVFYSMF
                     GFQRTADAIWAAADQLARGFLIGATAGRTTLTGEGTQHMDGHSPMLAATNPAVVTYDP
                     AYGYEIAHIVKSGLERMYGPKPEDVIFYLTVYNEPMPQPAEPENVDAEGIVKGMYLLK
                     EGSFDGVGADARRAQLLASGVGVAWALEAQELLKRDFGVVADVWSVTSWNELRRDGLA
                     CDEEKFLNPGADNPVPWVTQKLSGRPGPVIAVSDYMRQVQDQIQNWVPGEFASLGADG
                     FGFSDTRAAARRFFHIDGPSLVVRTLQQLAASGEVDPSWPARAAEQYRLKDVTAGSSG
                     SVGGDA"
     gene            complement(6495..7502)
                     /locus_tag="PKJ61_07310"
     CDS             complement(6495..7502)
                     /locus_tag="PKJ61_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017934895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOC13670.1"
                     /translation="MDPVAALREIAYLMERVQADSHRVRAFRAAADVVEGLAPAEFGR
                     RAAAGTWQELPGLGASTAEVVSQAVAGRIPVRLARLRAEAKPLATGGETLRAQLRGDL
                     HTHTVASDGSAPIAQMAAVAAGLGYDYLAITDHSPRLRVANGLSAERLAAQLDEIDAF
                     NREAPLRVLKGIEVDILVDGRLDQTAAMLQRLEVVVASVHSELRMPSDQLTRRMVAAV
                     AHPRTNILGHCTGRLITGSRGTRPSSQFDAEIVFEACRQFNVAVEINSRPERTDPPDE
                     LLEVAVATGCVFAIDSDAHAPGQLEFPAYGAARAEAAGIPPERIVNTWPVAELLAWTR
                     G"
     gene            7548..7892
                     /locus_tag="PKJ61_07315"
     CDS             7548..7892
                     /locus_tag="PKJ61_07315"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HOC13671.1"
                     /translation="MGEFTDLIAANEGTTAAALQHILNLALAEVPDAVEDLSYGTPAL
                     RYRGKPLIGVRVSAKHLSIFPFSPEVVDAVADQLGGYSLSKGTIRFTADRPLPEVVVT
                     RVVALRKAEIER"
     gene            7889..9073
                     /locus_tag="PKJ61_07320"
     CDS             7889..9073
                     /locus_tag="PKJ61_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOC13672.1"
                     /translation="MTTAPGSDGWPSAAKRVEISRPLLAASGELTTAALARIAAEHPW
                     FAELDAENRSWISLVAKSGIDGFLTWFASGGERLSPGNVFDAAPRALTRQITLNQTVD
                     LVSTTIDTVEDRIDTLPEADQGVLRIGILRYSREVAFAAARVYARAAESRGAWDARLE
                     AMAVDAVLRGDADESVVSRASTLGWASPAGIVVVIGSVPDDPESAIQSIRISASKNSL
                     DLLAAPQGDRLVLVIGGQLESTAESLRVVAGFADQFGDGQIVVGPIVSDLVEAARSAR
                     AALSGMRAAKAWPEAPRPVAAADLLPERALAGDGHARRTLATDIYQPLAAAGSELVDT
                     LAAYFDTSGSIEATARAKYVHANTVRYRLRRVQEITGYSPHDARDAYALRLALTLGRL
                     LS"
     gene            9243..10442
                     /locus_tag="PKJ61_07325"
     CDS             9243..10442
                     /locus_tag="PKJ61_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020577561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HOC13673.1"
                     /translation="MLAIVAPGQGAQTPGFLAPWIAEPALADRLNWLSTVAGIDLADY
                     GTNADAETIRDTAIAQPLLVAAGLVTALQLFPSPADGFRRTAVAAGHSVGEITAAAAT
                     GVLSAEQAMVFVRERGRQMAVASAARPTSMMAVVGGDPAEVLAAIEAAGLTPANNNGS
                     GQIVAAGTVEQLAELSANPPARARLIQLSVAGAFHTVHMQPAVDHLARLAKAMTTRDP
                     RTRLLSNTDGQVVQNGREYLGRLVKQVANPVRWDLCMQTMADLGITGLLELPPSGTLT
                     GIAKRNLKGVELFSLNTPDQIPAAQDFVAKHGDVGSAMASTPSWMLVVSPSKGEFRKA
                     DGLVPNSQLAGNTVIGTVANLRDTTSVATSNAGTVTEWLVEDGDPVSPGQPLLRLYPT
                     QDSTGAA"
     gene            10439..11434
                     /locus_tag="PKJ61_07330"
     CDS             10439..11434
                     /locus_tag="PKJ61_07330"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004811612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HOC13674.1"
                     /translation="MTEIKASTGPKYARILSVGSAVPSKVIDNEHLLQYIESSEEWIT
                     QRTGIKERRWIAEDESIETLSLAAAEQAIERAGISVDQIDAVILSTISHYHQTPALAP
                     QLATKLGIPDAAAYDISAACAGFSYGVAQAEALVRSGQARHVLLIAGETISEITDLTD
                     RGTAFLFGDGAGAVVVGPSETNGIGPVVWGSDGSQAGTIWQTTDWHTAVEQGTWPKLG
                     MDGRAVFKWATGFIAQAAKKCLDEAGLTPDDLDVFIPHQANDRITDTLLRYLKLPEKV
                     VVARTIRQFGNNSAATIPMAMAELLESGQAKSGQTALIIGFGAGLVYSGQVITLP"
     gene            11490..11732
                     /locus_tag="PKJ61_07335"
     CDS             11490..11732
                     /locus_tag="PKJ61_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOC13675.1"
                     /translation="MATTEEIRAQLAELVNDVAGVPVEDVQLDKSFVDDLDVDSLAMV
                     EILVGCEEKFGVTIPDEESKNLKTVGDAVAYIEAHS"
     gene            11911..13173
                     /locus_tag="PKJ61_07340"
     CDS             11911..13173
                     /locus_tag="PKJ61_07340"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002544946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HOC13676.1"
                     /translation="MIEIVITGLGATTPLGGDLATTWAGLLAGRSGVRAITEEWAEEL
                     PVKIAAYVAVDPSEVIDRVKARRLDRSTQLALIAAREAWTDAGFDWEAEEGSQVDPQR
                     LSVSLASGIGGLHSLLNNWDAHRDKGYRRVSPFTIPMLMANAPAANVGLAVHAKAGVH
                     TPVSACASSNEAISLALDQLRLGRADIAVVGGTEATIHPLPLAAFGQMQALSRRNDDP
                     EGASRPWDRGRDGFVMGEGSAVMVLETLAHAKARGAKIYGTLAGAGITADSHDIVQPD
                     PTGEGQSGAMIKALADSGLSAADIKHVNAHGTSTPQGDLTEAGSIRTGLGEAVDSCVV
                     TSTKSMTGHLLGGAGALETIATVLALRDHRVPPTINLEDPEPNLGIDIAANVARSLPS
                     GDLAAINNSFGFGGHNVAITVTNANATV"
     gene            13196..14620
                     /locus_tag="PKJ61_07345"
     CDS             13196..14620
                     /locus_tag="PKJ61_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HOC13677.1"
                     /translation="MTQATAPAKPARLPRAEDPRNPNFRLAALLDAGSLGLISPDDNS
                     GMLAATGSINGSPVVAFCSDATVMGGAMGVQGCEVVVQAYRRALVDRVPIIGIWHSGG
                     ARLAEGVLSLHAVGRIFHAMTQASGKVPQISVVLGAAAGGAAYGPALTDVVVLAPEGR
                     IFVTGPDVVRSVTGEDVDMLRLGGPDTHERKSGVVHVVADDEADALGRARKLTTLLAA
                     QGGISGEVPDTDLASLLPESAKRAYDVHPLINGLLDEGSVMELHARWAPNVTVAFGRL
                     GGRTVGVVANNPLRLGGCLDSLSAEKAARFVRLCDAFGVPLVVLVDVPGYLPGVGQEW
                     DGVVRRGAKLLHAFAEASVPRVTLVTRKAYGGAYIAMNSRSLGATKVFAWPAAEIAVM
                     GPVAAIRILHRRKLAEVAPDMLAQVEAELAAEHERIAGGVDKAVEIGVVDQIISPVAT
                     RSALAEAIRAADTGVRGSHTNIPL"
     gene            complement(14626..15123)
                     /locus_tag="PKJ61_07350"
     CDS             complement(14626..15123)
                     /locus_tag="PKJ61_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020385816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3145 domain-containing protein"
                     /protein_id="HOC13678.1"
                     /translation="MSGTRGIVWIHSAPAALRPHLEWALGAVLGAPVELSWSEQPAEP
                     RTLRAEYHWHGPAGRGAELASALRRCVRARFEVTEEPTAGTEGSRWAYTPRLGVFSAT
                     IGVHGDVMIHEDRLKQALLADALGQRPLGEGVAELLGAAWDAELEAFRYAGDGAPVRW
                     LYQVG"
     gene            15373..16104
                     /locus_tag="PKJ61_07355"
     CDS             15373..16104
                     /locus_tag="PKJ61_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016455725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOC13679.1"
                     /translation="MEPIAANLSAIRARIDAASAASGRAPGSVRLLPVSKTRPLPDLQ
                     AAIEAGCTRFGENKAQELEARSFALADVPGLEWVMIGHLQSNKAKLVARYASEFQALD
                     SVPLAAELDRRLQPLGRRLDVLIQVNSSGEESKFGLPPDEVVAFARELTTFDALRVRG
                     LMTLALPSAVKPQVAACFARMQLVQQLLREAGIAGQSYNELSMGMSGDFELAIAHGAT
                     QVRIGTGIFGARDYGPNDDRDGDQE"
     gene            16101..16565
                     /locus_tag="PKJ61_07360"
     CDS             16101..16565
                     /locus_tag="PKJ61_07360"
                     /inference="COORDINATES: protein motif:HMM:NF002696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-degrading domain-containing protein"
                     /protein_id="HOC13680.1"
                     /translation="MSALTAQLEAEALELRFGDFTLDDAWELGCSMRAAAADADLPIA
                     IGIVLGEQRVFHTALPGSSADNDDWLARKTRVALRYGKASLAVGEAFRDRGGDFDTDG
                     RLDVARYAAAGGVVPIRLAGGTVIGAVGVSGLPQLEDHAFVVAQLRAFLADR"
     gene            complement(16583..17551)
                     /locus_tag="PKJ61_07365"
     CDS             complement(16583..17551)
                     /locus_tag="PKJ61_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002521722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOC13681.1"
                     /translation="MALMLDLEFMRPERLWWLVLIPLLVGLYVLLLWRRRAKARPHAI
                     TNLERVLPKQQAWKRHAAVGAAVLSLAALNLAFAQPKGEVEVPRERATVVLTMDVSRS
                     MLAEDVAPNRLEAAKQAAQDFLTMLPVGFNVSLVAFAGYPTILVPPTTDRGVVAAAIS
                     SLQVAPSTATGDAIFASLEALEQAPPDPDNPDEPTPGAIVLLSDGSSNNGRPPTQAAQ
                     EAKKRKTPVHTIAFGTAGGYVETSGRREAVPADPEQMAQIARISGGKTFTAGSLGELK
                     EVYSAIAKSVGYVKVDQEVTEQYAGFALAFAVLAAFAMISLGARWP"
     gene            complement(17542..18504)
                     /locus_tag="PKJ61_07370"
     CDS             complement(17542..18504)
                     /locus_tag="PKJ61_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOC13682.1"
                     /translation="MIEWPPTFVNPERLWVLALIPLMIAGYIFLVLRKKKTGMRFTNT
                     SILGRVVPKQSQWRRHLAVALSLAAMFALSFAWARPNGIEMVPRERATVVLVIDVSQS
                     MQATDVKPSRLDAAKAAALTFIKALPSQYNVSLVSLAGNPSVRLPPTTDRVQATQAIN
                     GLKLQDSTSVGEAVYTALAALQLAPKGTDSSPAPGAVVLLSDGQNTAGRSPMQSAAEA
                     KTQGVPIYTIAYGTDNGYVDLDGKRERVPPDKELLTNIANTSGGQMYTAEDLGQLDKV
                     YNNIRSEVGQTPVKKETTALWAGYGLAFAVVAALAAVSLGARWP"
     gene            complement(18501..19502)
                     /locus_tag="PKJ61_07375"
     CDS             complement(18501..19502)
                     /locus_tag="PKJ61_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOC13683.1"
                     /translation="MGSVLAEPKSATIPLHKLAPEAALRRLELTIVRRLEGFLHGDHQ
                     GLLPGPGSEANDARAYVPGQDDVRKIDWAVTARTTVPHVRDTIADRELEIWALLDVTP
                     SMNWGTEGVTKRDLGIAAIATIGFLSQKMGDRFGGLIMRPDEIKRLPAQSGRTALYGL
                     LRRMLTEPIVSDHTTGPYTLAAGVQRLARAERRRGMRVVVSDFLTPGDFELDANVAPE
                     WERPMRELAVRNQVLAVEVVDRHEVEFADVGELLIRDPETDFERYVNTSDEAARERMD
                     AATAAQRERVRVALRRAGAAHIQLRTDRDWVHDIARFVLAYRRTAGMLHQPPAGVTK"
     gene            complement(19553..>20332)
                     /locus_tag="PKJ61_07380"
     CDS             complement(19553..>20332)
                     /locus_tag="PKJ61_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015070208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOC13684.1"
                     /translation="IYSARQEKFDIALGPVFVNFVLADEINRAPAKVQSAMLELMAEQ
                     QVSIGGHTYKVPQPFIVIATQNPIESEGVYPLPEAQRDRFLLKVDVPYPRGNEEFEIL
                     RRMSVRPPSANPVLEPETILKLQEQASNVFVHNLVAEYVVRLVLATRTPEEFGMPDLT
                     SVIQVGCSPRATLGLVAAARALALIHGRDYVLPTDVQAVARDVMSHRMVLGFDAVADN
                     ITSAQVVERIVAMVPPPTPVWHNQQRQAAHTQHRYAPEARS"
BASE COUNT         3138 a         7171 c         6844 g         3179 t
ORIGIN      
        1 gcagagcgaa agctcgtggc gggcaactcg actttctggg cccgaatcgt gggcaaatcc
       61 gacggcgcgc ggacgccccc ggtctggccc taagatgaac cactgcgggc gcatagctca
      121 gttggttaga gcacctccct tacaaggagg gggtcggggg ttcgagtccc tctgcgccca
      181 cccgaggccc atgcgcgaca tcggtgacac ctgaatcgcg actctggcac gatttcgcca
      241 tgagcgccca cctccccagc gccggccgag cccggtggcg accctggtgg gcgatcgccg
      301 ttgccatcgc gacgctcggt gcaggcgcat tggtgctctg gtggggcgtg gcagccatcg
      361 cgaccgaccg acccaatcag tggttcagcg gcttcggcac ggtgctgggc accgcagcca
      421 tcgtcgcagc cctgccaccg atggcctgcc tgacggtctt cctggccagg gacacgcgag
      481 ggtggttctg ggccggcacc ggtctggccc tcaccgtggc cgcggtcgtg atcagcgtgg
      541 tcatcggcga ttcgaacttc aggtgagctg catcccttcc gtacagccgc cgacctacag
      601 ctaggctgaa caggtcaggt cacgcgcgcg atggggctcg aatggttggt tacctcggac
      661 tgggcgaacg gttgggcgcc taccgggtgg acgccgaact cgggcgtggc ggcatgggca
      721 ccgtgtatcg agccactcac ctcccgctca accggcccgt agccctcaag attctcgacc
      781 cgcaactcgc agccgacccg gtcttcgtcg aacgcttcca ccgcgaggcc gcgatcctgg
      841 ccagcctcga ctcaccacat atcatccgca tcttcgacca tggcgaaatc gacggacacc
      901 tgtacttggc cacgcagtac gtcgccggcg gcgatctgca cgagttggtc cgcggttccg
      961 ggccgctgcg ccccgagacc gcacttcagg tttacgggca gatcctctcc gcggtcggcg
     1021 acgcccacaa gcgcggcatc gtccatcgcg acgtcaagcc cagcaacgtg ctgctgcatc
     1081 ctgacgtggt ggagcccttc gcctaccttt gcgatttcgg catcgcccag tcgaaggaag
     1141 ccgggctgac cgaagccggc gccgtcgccg gcagttgggc ctacatgagt ccggaacggc
     1201 agaccgggtc gccggccacg ccgcagagtg acatctactc ggcggcctgc gtcctttggt
     1261 tcatgctcac cggacgcggc gtctacaccg gcacggacgt cgaagtcgcc atggcgcacc
     1321 tccactccgc agtcccccag ttgcgcggcg tcgaccccct gctcgtcgag ctgaaccggc
     1381 tcttccgcac ctgcctggcc aaggatcccg ccgagcgtcc ggccaccgcg gcggaggcac
     1441 tcgcgctgtc ccgcgattgc cagccgctgg ctgttggcac catcccgctc gacctgaaca
     1501 gccccgactc cggcatcttc cgaaccaccc taaagcccgg cgatcccggg cccctcgccg
     1561 acccggcgcg ccgcagcgga cgccgcgtcg gcctgctcgt cgccgctggg gttgccgccc
     1621 tcgccgtggg tgccacggcc accatgctcg tactgccgac ccaggcccgc tccgcgttga
     1681 ccgcggtcgc ccagccgggc gcgagcccgt ccggcccgtc cgccagttcc gcgtcagatc
     1741 cgcccgcacc cggttacctg tgctgggacg cccaaccggc cgcagacctg tccgcatgca
     1801 ccaacccgtc cggacgctcg ggcctgcgct acctctatcc gtcgctgggc gaacagtggg
     1861 cggactgcaa gtacgtcgcg taccgcagca ccacggacgc ttatgactgc acgatcaagc
     1921 atctcggcct gatccgctac cgctactgga cggatgtcgc cgaggccaac cagcactact
     1981 cgcagaagta tgcgaaagcc accgagaccg atctggtgct ggacggcaac gtggtcggca
     2041 ccctctaccg cctgccacga ccggtggcag gcgagtaccg catgtccggc ttctggctgg
     2101 acggacactt cagcttcagc gtggaggcgc cgacgaaggc ccagcgcgag cagttgttca
     2161 agcttgtcca gctccgcggc tacgaccagc tgaccggcaa gcgaaaaggg gccgacccga
     2221 cgatcggcgt gctcagctga gcttcgcctt gagttgcgcg atcgtttctg ccgacagtcc
     2281 caaccccttg cccaggtagc cgtcgagccc gccgtactgg cgatccatcc gggtcagcgc
     2341 tgcgttgagg taggccggtt ccactgtcag cagcgcacgc ttcgccttcg cggccgtgcg
     2401 accctgcctg aacagagtcg cggagtacgc cttcgcgatc gccgcttcgc gatactcgtt
     2461 cgacttcagg tactcggcga gcacctgctc gcggtcggcg ccggcgatca gctgcagcag
     2521 cgcagcgagc cagccggtac ggtccttgcc ttcggtgcag tggatgatca ccggcccctt
     2581 ggctgcggcc acgatcttca gcgccttcgc gatctgcttg cgctgcgccg acttggcgac
     2641 gaagtcggtg ttcaccttct gcatgtgcgc cttcgccgcc gccacactcc gggtcggtgg
     2701 cgcgggcgtc cggtagaccg cgtagacgtt cgcagagtgc cgcttggtgc cagggatctt
     2761 gcggtcggga gcggcctttg ccaccgcggg cgtgcgcaga tcgacgatgt cggtgatccc
     2821 ggccgccacc aggtacgcac ggtcggacgc cgacagcgtg gccagcttgc cggagcggta
     2881 gaccactccg atggtcatcg tccggccgcc tggcagcgcc aagcccggcc cggccacgtc
     2941 cctgaagttg gttactgact tgatcggcag cgtcgcgacc gacgccgacg ccacctggcc
     3001 ggcagactgc gtccaggatg ccgagtagtc gctcaccacc gggacggcgg cgcagcccga
     3061 accggccagc cccaccgcga tcgctgcggc cagcccgcgt ccgaatcgaa cgctaacggc
     3121 gcccgccctt gggacggacc agcttctgcg cctgccagtt gcgagagacc gaagcggtgt
     3181 tcgccagcgc caggcctgcg gtcagcgcgg cctcggccag atcggtcggg tcgacgtaac
     3241 cgtcccggcc cacccgcggg gtgagcaacc agatgtgtcc ccgctcggtc aggtcggtga
     3301 gcgcgtccac caagccgtcg gcaaggtcgc catcctcgtc gcgccaccac agcaccacga
     3361 cgtcgaccgc ttcgatcgct tcgtagacga aatcctcacc gagctcgtcg aggagcgcgc
     3421 tgcgcagacc ctcgtcggcg tcctcgtccc agccgagctc ctggactgcc tgtcccttgg
     3481 cgattccgag cgcggccacg atcggcgctc gcgtcgttgc accgggcgcg tcactcaccg
     3541 cgtctggacc ccactttcgc gctagttgtt cctggctttc agcctgccag aaaccggccg
     3601 gggatcaaag cggacgggct caggcgtcgc cgccgaccga gccggagcta cccgcggtga
     3661 catctttgag ccgatactgc tcggcggcgc gggccggcca gctcggatcg acctcaccgg
     3721 acgcggccaa ctgctgcagc gtccgcacca ccagcgacgg accgtcgatg tggaagaacc
     3781 gccgcgctgc cgcccgggtg tcggagaagc cgaagccgtc cgcgccgagt gaggcgaact
     3841 cccccggcac ccagttctgg atctggtcct gcacctggcg catgtagtcg gagaccgcga
     3901 tcaccgggcc gggccggccg gacagcttct gcgtgaccca cggcaccggg ttgtcggcac
     3961 cggggttgag gaacttctcc tcgtcgcagg ccaggccgtc tcggcgcagc tcattccagc
     4021 tggtcacgct ccacacgtcc gccaccacgc cgaagtcgcg cttcagcaac tcctgcgcct
     4081 ccaatgccca ggccacgccc acgccggacg ccagcagctg cgcgcggcgg gcgtcggcgc
     4141 cgacaccgtc gaagctgccc tccttgagca ggtacatgcc cttgacgatg ccctcggcat
     4201 cgacgttctc cggctcggcc ggctgcggca tcggctcgtt gtacacggtc aggtagaaga
     4261 tcacatcctc cggcttcggc ccgtacatcc gctccaggcc gctcttcacg atgtgcgcga
     4321 tctcgtagcc gtaggccggg tcgtaggtca ccaccgccgg gttcgtcgcc gccagcatcg
     4381 ggctgtggcc atccatgtgc tgggtgccct caccggtcag cgtggtgcgt ccggcggtag
     4441 cgccgatcag gaagccgcgg gccagctggt cggccgcggc ccagattgcg tccgccgtcc
     4501 gctggaagcc gaacatcgag tagaagacgt agatcggcac catctgcaga ccatgcgtgg
     4561 agtacgcgct gccgaccgcc tggaacgcgg ccaccgaacc ggcctcgttg atgccggtgt
     4621 gcaggatctg gccggtgcgg gcctcgcggt aggcgagcat cagctcgtgg tccaccgggg
     4681 tgtagttctg cccgtgcggt gagtagatct tgatggtcgg gaagaacgag tccatgccga
     4741 acgttcgggc ctcgtccggg atgatcggca ccacgtgcgg cgcgaactcc ttgtcgcgca
     4801 tcaggtcctt cagcaggcgg acgaaagcca tcgtggtggc cacggcctgg ttgcccgaac
     4861 ccttcttcac gccggcgtac gccgcttcgt cgggcagctt caacggctgc ggctggtaac
     4921 gccgctgtgg cacggggccg ccgagggccc ggcggcgctc cagcacgtac tggatgcgct
     4981 cgtcgccggg gcccgggttc acgtacggcg gctggtaggg attctcctcc agcaccgcat
     5041 cggtgatcgg catctccagg cgatcccgga actgcttgag gtcgtcgagg gccagcttct
     5101 tcatctggtg ggtcgcgttg cggccggcga agtggctgcc gaggaagtag cccttgatgg
     5161 tgtgcgcgag gatgaccgtc ggcgcgccga cgaactgggt ggcagcctgg taggccgcgt
     5221 agaccttgtg gtagtcgtgg ccgccccggg tcagcttcca gatccggtcg tccgaccagt
     5281 tctccaccat cttcgcggtg cgcggatcac ggccgaagaa gttgtcgcgc acgtaggcgc
     5341 cgtcgttggc cttgaaggtc tggtagtcac cgtcggtgaa cgagttcatc acgttgacca
     5401 gtgcgccgtc gcggtcggtg gccagcagcg gatcccagcc gcggccccag acgaccttga
     5461 tcacgttcca gccggcgcca cggaagaagc tctccagctc ctgcatgatc ttgccgttgc
     5521 cgcgcaccgg gccgtcgagg cgctgcaggt tgcagttgac cacgaaggtg aggttgtcca
     5581 ggccttcgta gccggccagc tgcagggcgc cacggctctc cacctcgtcc atctcgccgt
     5641 cgccgaggaa ggcccacacc cgctgctgcg cggtgtcctt caagccgcga ttgtgcaggt
     5701 acttgttgaa ctgcgcctgg tagatggccg cgatcgggcc caggcccatc gacacggtcg
     5761 ggaactgcca gaagtcgggc atctgccgcg ggtgcgggta ggacggcagc gcgcgcacgc
     5821 ggccgtcgac gatgtggctc ttctcctggc ggaagccgtt caggtcgttc tcgtccagac
     5881 gcccctcgag gaaggcgcgg gcgtacatgc cggggctggc gtggccctgg aagaacacct
     5941 ggtcgccgcc gcccgggtgg tcctggccgc ggaagaagtg gttgaagccc acctcgtaca
     6001 gggtcgcgga cgacgcgtag gacgagatgt ggccgccgac gccgacgccc ggacgctggg
     6061 cgcggtgcac catgatcgcg gcgttccagc gagccagacg gcggaacttg cgctccagtt
     6121 cgacgtcgcc gggataccag ggctcacgct ccggcggaat ggtgttcaca taatcggtgg
     6181 cagtcagcga aggcagaccg aggttctggt tgcgagcccg ttcgagcagc cgcagcatca
     6241 cgtaccgagc gcggttgcgg ccgtcctgct cgatcatctg gtcgagggac tccagccatt
     6301 cggcggtctc gtcggagtcg atgtccggca ggttcgtcgg cagtccgttc aggatcgggc
     6361 cgacagagtt acgacttgcc acagctgtgc ctctcttttc ttgcacaaga tcgttgcgcc
     6421 atcccgaccc taccgggagt aaccgtggac agcgccatcc gcgatccagc attagagcgg
     6481 gcaatctgtc gggctcagcc tcgcgtccag gccagcagct cggccaccgg ccaggtgttc
     6541 acgatgcgct cgggggggat cccggccgct tccgcgcggg ccgcgccgta ggccgggaac
     6601 tccagttggc caggcgcgtg ggcgtcggag tcgatcgcga acacgcaacc ggtagccacc
     6661 gcaacttcca gtagctcgtc cggcggatcg gtgcgttcgg gacgagagtt gatctcgacc
     6721 gcgacgttga actggcggca ggcttcgaag acgatctcgg catcgaactg gcttgacggg
     6781 cgagtgccgc gcgaaccggt gatgagccgg ccggtgcaat ggccgaggat gttcgtgcgc
     6841 gggtgggcga cggccgcgac catccggcgg gtgagttggt cggacggcat ccgcagttcc
     6901 gaatggacgc tggccaccac cacttcgagt cgttgcagca tcgccgcggt ctggtcaagc
     6961 cggccgtcga ccaggatgtc gacctcaatc cccttcagca cccggagcgg agcctcgcgg
     7021 ttgaacgcgt cgatctcgtc cagttgcgcg gccaggcgct cggccgacag cccgttggcc
     7081 acccggagcc ggggcgagtg atcggtgatg gccaggtagt cataacccag gcccgccgcg
     7141 accgcggcca tctgcgcgat cggcgcgctg ccgtccgaag caacggtatg cgtgtgcagg
     7201 tcgccgcgga gctgggcacg taacgtctcg ccgccggtgg ccaacggctt ggcctcggcg
     7261 cggagtctgg ccaggcggac ggggatgcgt cccgctacgg cctggctgac cacctctgcg
     7321 gtgctcgccc cgaggccggg cagctcctgc caggtacccg cggcggcgcg gcgtccgaac
     7381 tccgccgggg cgaggccctc gaccacgtcc gcggcggcgc ggaacgcccg cacccggtgg
     7441 ctgtcggcct gcacgcgttc catcaggtac gcgatctccc gcagtgcggc taccggatcg
     7501 attggcaccc gcccagccta tgggtgccct ggccgtaagc tctgcgcatg ggcgagttca
     7561 ctgacctcat cgccgccaac gaaggcacga ccgcagcagc actccagcac atcctcaacc
     7621 tggcgctcgc cgaggtaccg gacgcggtcg aggacctcag ctacggcacc ccggcgctgc
     7681 gctaccgggg caagccgctg atcggcgtgc gggtgagcgc caagcacctc tccatcttcc
     7741 cgttcagccc cgaagtggtg gacgcggtgg ccgaccagct cggcggctat tcgctgtcga
     7801 agggcaccat ccgattcacc gccgaccgtc cgctgccgga agtggtggtc acccgcgtgg
     7861 tggccttgcg gaaggcggag atcgagcgtt gaccactgcg cctggtagcg atggctggcc
     7921 gtccgcggca aagcgcgtcg agatctcgcg gccgctgctg gccgcgtccg gtgagctgac
     7981 caccgccgca ctggcgcgga tcgccgccga gcatccgtgg ttcgccgaac tggacgccga
     8041 gaaccggtcg tggatcagcc tggtggcgaa gtcgggcatc gacgggttcc tgacctggtt
     8101 cgcgtccggt ggcgagaggc tctcccccgg gaacgtgttc gacgccgcgc cgcgcgcgct
     8161 cacccgccag atcacgctga accagacggt cgatctggtc agcaccacga tcgacaccgt
     8221 ggaggaccgc atcgacaccc tgccggaagc cgaccagggc gtgctgcgaa tcgggatcct
     8281 gcggtactcc cgggaggtgg cgttcgcagc cgcgcgggtc tacgcccggg cggctgagtc
     8341 ccgcggcgcg tgggacgccc gcctggaggc gatggccgtg gacgcggtgt tgcgtggcga
     8401 cgccgacgaa tcggtggtct cgcgcgcgtc caccctcggc tgggcgtcgc cggctggcat
     8461 cgtcgtggtg atcggcagcg tgcccgatga cccggagtcg gccatccagt cgatccggat
     8521 cagcgcgtcc aagaactcac tcgacctgct ggccgccccg cagggcgacc gcctggtgct
     8581 cgtgatcggc ggccaactgg aatccacggc tgaatcactt cgagtggtgg ccggtttcgc
     8641 cgaccagttc ggcgacggcc agatcgtggt cggcccgatc gtctccgacc tggtcgaggc
     8701 ggcccgcagt gcccgggccg cgctgtcggg catgcgcgca gccaaggctt ggccggaggc
     8761 gccgcgtccg gtggcggcgg cggacctgct gcccgaacgc gccctcgccg gcgacggtca
     8821 cgcccgacgc acgctcgcca ccgacatcta ccagccattg gcggcggccg gcagtgagct
     8881 cgtcgacacc ctcgcggcgt acttcgacac gtccggttcg atcgaggcga cggcgcgggc
     8941 gaagtacgtc cacgcgaaca ccgtccgcta tcggttgcgc cgagtgcagg agatcaccgg
     9001 gtattcgccc cacgatgcca gggacgcgta cgccctacgc ctggcgctca ctcttggccg
     9061 actgctgagc tagcgtcctc agcctctcag gccaatatca ggctcgggcg gtgtccacaa
     9121 cgaccaagcc tgccaaagcg gtgcaacggg cgtgtcgatt gtaggaaacc tacaaagagg
     9181 accgttatga attcgttgcg tttcgcgccg gttgctgtcg cccgatcagc cagagtggac
     9241 tcgtgctcgc aatcgttgcg cccggacagg gcgcccaaac cccggggttc ctggcccctt
     9301 ggatcgctga acccgccctg gctgatcgcc tgaactggct cagcaccgtc gccggcatcg
     9361 acctcgccga ctacggcacc aacgccgacg ccgagaccat ccgggacacc gccatcgccc
     9421 aaccgctgct ggtcgcggcc ggcctggtca ccgcgctgca gctcttcccg agccctgcgg
     9481 acggtttccg ccgcaccgcg gtcgcggccg gacacagcgt cggtgagatc accgcagctg
     9541 cggccaccgg cgtgctgtcg gcggagcagg ccatggtgtt cgtgcgggag cgtggccggc
     9601 agatggcagt cgccagcgcg gcccggccga cttcgatgat ggcggtcgtc ggtggcgacc
     9661 ccgcggaggt gctggctgcg atcgaggccg ccgggctgac ccccgcgaac aacaacggct
     9721 ccggccagat cgttgccgcc ggcacggtcg agcagctcgc tgagttgtcc gccaatccgc
     9781 cagcccgtgc ccggttgatc cagctgagcg tcgccggcgc cttccacacc gtccacatgc
     9841 agcccgcggt cgaccacctc gcccggctgg cgaaggcgat gaccacccgt gatccacgca
     9901 cccggctgct gtcgaacacc gacggccagg tggtgcagaa cggtcgcgag tacctcggcc
     9961 ggctggtgaa gcaggtcgcg aaccccgtcc gctgggatct gtgcatgcag accatggccg
    10021 atctgggcat caccggcctg ctggagttgc cgccgtccgg cacgctgacc ggcatcgcca
    10081 agcgcaacct gaagggcgtc gagctgttca gtctgaacac ccccgaccag atcccggcag
    10141 cgcaggactt cgtggccaag cacggcgacg tcggttcggc gatggccagc accccgtcct
    10201 ggatgctggt ggtctccccc agcaagggcg agttccgcaa ggctgacggc ctagtgccca
    10261 actcccaact cgccggcaat acagtgatcg gcaccgtggc caacctgcgc gacaccacgt
    10321 cagtggccac cagcaatgcc gggaccgtta ccgaatggct ggtcgaggac ggtgacccgg
    10381 tctcccccgg gcagccgctc ctccgcctct accccactca ggactccaca ggagcagcat
    10441 gaccgaaatc aaggccagca ccgggccgaa gtacgcccgc atcctttcgg tcggctcggc
    10501 cgtgccgtcg aaggtgatcg acaacgagca cctgctgcag tacatcgaat ccagcgaaga
    10561 gtggatcacc cagcgcaccg gcatcaagga acgccgctgg atcgctgagg acgagagcat
    10621 cgagaccctc tcgctggccg cggctgagca ggcgatcgag cgggccggca tcagcgtcga
    10681 ccagatcgac gcggtgatcc tgtccacgat ctcgcactac caccagaccc cggcgctcgc
    10741 cccgcagctg gccacaaagc tcggcatccc ggacgccgcc gcctacgaca tctccgccgc
    10801 ctgcgccggc ttcagctacg gcgtggccca ggccgaggcg ctggtgcgca gcggccaggc
    10861 gcggcacgtc ctgctgatcg ccggcgagac gatcagcgag atcaccgacc tgaccgaccg
    10921 tggcaccgcc ttcctgttcg gggacggcgc cggcgcggtg gtcgtcggcc cgagcgagac
    10981 caacggcatc ggcccggtgg tctggggctc ggacggcagc caggccggca cgatctggca
    11041 gaccaccgat tggcacaccg cggtcgagca gggcacctgg cccaagctgg gcatggacgg
    11101 tcgcgcggtc ttcaagtggg ccaccggctt catcgcgcag gcggccaaga agtgcctgga
    11161 cgaggccggg ctcacccccg acgacctgga cgtgttcatt ccgcaccagg ccaacgaccg
    11221 gatcaccgac accctgctgc gctacctgaa gctgcccgag aaggtcgtgg tggcccgcac
    11281 catccgccag ttcggcaaca actcggcggc gaccatcccg atggcgatgg ccgaactgct
    11341 ggagagcggc caagccaaga gcggccagac cgcgctgatc atcggtttcg gcgctggcct
    11401 ggtctattcc ggccaggtca tcacccttcc ctgaatcccc cggacaatcc ggaacaaaac
    11461 aagccccgca acaactaagg agaacccaca tggccaccac cgaagagatc cgcgcccagc
    11521 tcgccgagct cgtcaacgac gtcgccggcg tgccggtcga ggacgtgcag ctcgacaagt
    11581 ccttcgtcga cgacctggac gtcgacagcc tcgcgatggt cgagatcctc gtcggctgcg
    11641 aggagaagtt cggcgtcacg attcccgacg aggagtccaa gaacctcaag accgttggcg
    11701 acgccgtcgc ctacatcgaa gcgcacagct gactcgacgc tgattgagct tgtcggggcc
    11761 ctgaacgctc cctgaaacac ccttcgtaga cgctccctga gcctaacgaa gggtgcttca
    11821 ggcgggcctt cgacaagctc agccaccgtt cgccggtcgg gtcccgatga ctcgaccacc
    11881 cctaccgaac acctaccttc ggaaagaacc atgattgaga tcgtgatcac cggcctcggc
    11941 gccaccaccc cgctcggcgg cgacctggcc accacctggg ccggcctgct ggccggacgg
    12001 tccggcgtcc gcgccatcac cgaggagtgg gccgaggaac tgccggtgaa gatcgcggcc
    12061 tatgtcgccg tcgacccgtc cgaggtgatc gatcgggtga aggcccgccg gctcgaccgc
    12121 tccacccaac tcgccctgat cgcggcgcgg gaggcatgga ccgacgccgg cttcgactgg
    12181 gaagccgaag agggcagcca ggtcgacccg cagcggctga gcgtctcgct ggccagcggc
    12241 atcggcggcc tgcactcgct gctcaacaac tgggacgccc accgcgacaa gggctaccgc
    12301 agagtcagcc cgttcacgat cccgatgctg atggcgaacg cgccggccgc gaacgtgggc
    12361 cttgccgtcc acgccaaggc gggcgtgcac acgccggttt cggcctgtgc ttcctccaac
    12421 gaggcgatct cactggccct ggaccagttg cggctcggcc gcgccgacat cgcggtggtc
    12481 ggtggcaccg aggcgaccat ccacccgctg ccgctcgccg ctttcggcca gatgcaggcg
    12541 ctgagccgcc gcaacgacga tcccgagggc gcgtcccgtc cgtgggatcg tggccgggac
    12601 ggcttcgtga tgggcgaagg ctccgcggtc atggtgctgg agaccctggc gcatgcgaag
    12661 gcccgcggcg cgaagatcta cgggacgctg gccggcgccg gaatcactgc ggacagccac
    12721 gacatcgtgc agcccgatcc gaccggcgag ggccagtcgg gggccatgat caaggcgctc
    12781 gccgattcgg ggctgagcgc ggccgacatc aagcacgtga acgcgcacgg cacgtccacg
    12841 ccgcagggcg acctgaccga ggccggctcg atccgcaccg gccttggcga ggcggtggat
    12901 agctgcgtgg tcacctcgac caagtcgatg accgggcacc tgctcggcgg cgccggcgcg
    12961 ctggagacga tcgccaccgt gctcgccctg cgcgatcacc gggtgccgcc gacgatcaac
    13021 ctcgaagacc cggagcccaa cctgggcatc gacatcgccg caaacgtggc ccgcagcctg
    13081 ccgtccggcg acctggctgc gatcaacaac tccttcggct tcggcggtca caacgtggcc
    13141 atcacggtca ccaacgcaaa cgcgaccgtc tgatcgacga ctcaaggagc agcacatgac
    13201 ccaagccacc gcaccggcga agcccgcacg gctgccccgc gccgaggatc cgcgcaaccc
    13261 gaacttccgg ctggccgcac tgcttgacgc gggctcgctg ggcctgatca gcccggacga
    13321 caactcgggc atgctcgccg cgaccgggtc gatcaacggc tcaccggtgg tggcgttctg
    13381 ctcggacgca accgtgatgg gcggcgcgat gggcgtccag ggttgcgagg tggtcgtgca
    13441 ggcctaccgg cgcgccttgg tggaccgcgt cccgatcatt ggcatctggc attccggcgg
    13501 cgcccggcta gccgagggcg tgctcagcct gcacgcggtc ggtcggatct tccacgccat
    13561 gacccaggct tcgggcaagg tgccgcagat ctcggtggtt ctcggcgcgg ccgccggtgg
    13621 tgccgcgtac ggtcctgcgt tgaccgacgt tgtagtactc gcgccagagg gacgcatctt
    13681 cgtgaccggc ccggacgtcg tccgctcggt caccggcgag gacgtcgaca tgctgcgcct
    13741 cggcggcccg gacacgcacg aacggaagtc cggcgtggtg catgtggtgg ccgacgatga
    13801 ggcggacgcg ctcggccgcg cccgcaagct gaccacgctg ctggccgccc agggtgggat
    13861 ctccggtgag gtgcccgaca ccgacctcgc gtccctgctg ccggagtcgg ccaagcgcgc
    13921 ctacgacgtc catccgctga tcaacgggct gcttgatgag ggctcggtga tggaacttca
    13981 cgcacgctgg gccccgaacg tgacggtcgc cttcggccgg ctcggcggcc gcacggtcgg
    14041 cgtggtggcc aacaacccgc tgcgcctagg cgggtgtctc gattcgctga gcgcggagaa
    14101 ggccgcccgc ttcgtccggc tgtgcgatgc cttcggcgtg ccgctggtcg tgctcgtgga
    14161 cgtgcccggc tacctgcccg gcgtcggcca ggaatgggac ggcgtcgtgc gccgcggcgc
    14221 caaactgctg cacgcgttcg ctgaggcgtc cgtgccgcgg gtgacgctgg tcacgcggaa
    14281 ggcctacggt ggcgcctaca tcgcgatgaa ctcccgctcg ctcggcgcca cgaaggtctt
    14341 cgcctggccg gcagcggaga tcgcggtgat gggcccggtg gcggcgatcc gcatcctgca
    14401 ccggcgcaag ctcgccgagg tggccccgga tatgctggcg caggtcgagg ccgaactggc
    14461 cgccgagcac gaacggatcg ccggtggcgt cgataaggcc gtggagatcg gcgtcgtcga
    14521 ccagatcatc tcaccagttg ccacccgctc ggccttggct gaggccatcc gcgccgccga
    14581 caccggcgtc cgcggaagtc acacgaacat tccgctctag gcggctcagc cgacctggta
    14641 gagccagcgc accggagcac cgtcaccggc gtagcggaag gcttcaagtt cggcgtccca
    14701 ggctgccccc agcagttcgg cgaccccctc gccgagcgga cgctggccca gcgcgtccgc
    14761 cagcagggcc tgcttgagcc ggtcctcgtg aatcatcacg tcaccgtgca ccccgatggt
    14821 cgctgagaac accccgagcc gcggcgtgta cgcccagcgg ctgccctcgg taccggccgt
    14881 gggttcctcg gtaacctcga agcgcgcgcg gacgcagcgt cgcagggcgc tggccagttc
    14941 ggccccgcgc cccgccggac cgtgccagtg gtactcggcg cgcagtgttc gtggctcggc
    15001 cggctgttcc gaccagctca gttcgaccgg cgcgccaagg actgcgccca acgcccattc
    15061 caggtgcgga cgcaacgcag ccggcgcgga gtggatccaa acgatcccgc gcgtgccaga
    15121 catcagttcc ccaatcgccg agatatgcct tccccagcta cctcagcctc ttgggtgacc
    15181 ccattctgca cctcgggaca agcgcaggcc agccctgccc actccccttc cgtcctcgct
    15241 cccgaatggg acgtttactc ccgataattc gggagcgaga gtcccattcg ggagcgaagt
    15301 ccggagagtg ggtctcgccg gccagctggc gaggggcggg cgcggtccgc tgcttctggt
    15361 tagctagacc ccatggaacc gatcgccgcc aacctgagcg ccattcgagc tcggatcgac
    15421 gccgccagtg ccgcgtccgg acgcgcgccc ggttccgtcc gcctgctgcc ggtgagcaag
    15481 acccgtccgc tacccgacct gcaggcggcg atcgaggccg gctgcacccg cttcggggag
    15541 aacaaggccc aggaactcga ggccaggtcg ttcgctctgg cggacgtgcc cggcctggaa
    15601 tgggtgatga tcggccacct gcagtcgaac aaggccaagc tggtggcccg gtacgccagt
    15661 gagttccagg cgctcgattc ggttcctctg gccgccgagc tggatcggcg gctgcagccg
    15721 ctggggcgtc gtctggacgt gctgatccag gtgaactcgt ccggcgagga gtcgaagttc
    15781 gggctgccac ccgacgaggt ggtcgccttt gcccgggaac tgaccacctt cgacgcgctt
    15841 cgggtgcgcg gcctgatgac gctggcactg ccgtccgcgg tgaagcccca agtggcggcc
    15901 tgtttcgccc gcatgcagct cgtgcagcag ctgctgcgcg aggccgggat cgccggccag
    15961 tcgtacaacg aactatcgat ggggatgtcg ggcgacttcg agttggcgat cgcgcacggc
    16021 gccacccagg tgcgcatcgg gacgggcatc ttcggtgccc gcgattacgg gccgaacgac
    16081 gaccgggacg gagaccagga atgagcgcac tgaccgcaca acttgaggcc gaggcgttgg
    16141 agctgcgctt cggcgacttc actctcgacg atgcctggga gctgggatgc agcatgcgtg
    16201 cggccgcggc ggacgcggac ctgccgatcg cgatcggtat cgtgctcggc gagcagcggg
    16261 tcttccacac cgcgttgccc ggctccagcg ccgacaacga cgactggctg gcccgcaaga
    16321 cccgggtggc gctgcgctac ggcaaggcgt cgctcgcggt cggtgaggcc ttccgcgatc
    16381 gcggcggcga cttcgacacc gacggacgcc ttgacgtcgc ccgctacgcg gcggccggcg
    16441 gtgtcgtccc gatccgtctc gcaggcggca ctgtgatcgg ggcggtcggc gtgtccggac
    16501 tcccccagct cgaggaccac gccttcgtgg tggcgcaact gcgcgctttc ctggccgacc
    16561 gctaactggg ccacaccggg tcctacggcc agcgggcacc gagcgagatc atggcgaagg
    16621 cggcgagcac cgcgaaggcc agggcaaagc ccgcgtactg ctcggtgacc tcctggtcga
    16681 ccttcacgta gccgaccgac ttggcgatcg cggaatagac ctccttcaac tcaccgagcg
    16741 agcctgcggt gaaggtcttg ccgccagaga tccgcgcgat ctgggccatc tgctccgggt
    16801 cggcgggcac ggcctcacgg cgtccgctcg tctccacgta gccgccagcg gtgccgaagg
    16861 cgatcgtgtg caccggggtc ttccgcttct tggcctcctg ggcggcctgg gtcggcggcc
    16921 ggccattgtt gctggaaccg tcgctcagca gcacgatcgc gccgggcgtc ggctcgtccg
    16981 ggttgtccgg gtctggcggc gcctgttcca gcgcttccag ggaggcgaag atcgcgtcgc
    17041 cggtcgccgt cgagggcgcg acctgcaggc tggagatggc cgcagcgacc acgccccgat
    17101 cggtggtcgg cggcaccagg atcgtcggat agccggcgaa agccaccagc gagacgttga
    17161 acccgaccgg cagcatggtc aggaagtcct gcgcggcctg tttggccgcc tccaggcggt
    17221 tcggcgccac atcctcggca agcatcgagc gggagacgtc catcgtgagc accacggtgg
    17281 cccgctcgcg cggcacctcc acctcaccct tgggttgggc gaacgccagg ttcagcgcag
    17341 ccagcgagag caccgccgcg cctacggcgg catgccgctt ccacgcctgc tgcttcggca
    17401 gcacccgctc gaggttcgtg atcgcatgcg gacgcgcctt cgcccgacgg cgccacaaca
    17461 gcagcacgta cagccccacc agcagtggga tcagcaccag ccaccaaagg cgctccggac
    17521 gcatgaactc gaggtccagc atcagggcca cctcgctcca agtgaaaccg cagccagcgc
    17581 cgcgaccacg gcgaaggcca gaccgtagcc cgcccatagc gcggtggtct ccttcttcac
    17641 cggggtctgg cccacctcgc tgcggatgtt gttgtagacc ttgtccagct gaccgaggtc
    17701 ctcagcggtg tacatctgcc cgcctgacgt gttggcgatg ttggtcagca gctccttgtc
    17761 cggcggcacc cgttcgcgct tgccatccag atccacatag ccgttgtcgg tgccgtaagc
    17821 gatcgtgtag atgggcacgc cctgggtctt ggcctcggcc gccgactgca tcggcgaccg
    17881 gccggcggtg ttctgcccgt ccgacagcaa cacgaccgcc cccggcgcag gactggagtc
    17941 ggtgcccttc ggcgccaact gcaacgccgc cagcgcggtg tagaccgcct cccccaccga
    18001 ggtcgagtct tgcagcttga gcccgttgat ggcctgggtg gcctgcaccc tgtctgtggt
    18061 cggtggcagc cggacggacg ggttgccagc caggctgacc agggacacgt tgtactgcga
    18121 cggcagtgct ttgatgaagg tcagcgccgc cgccttggcc gcgtccagcc gggagggctt
    18181 cacgtccgtg gcctgcatcg actgggagac atcgatcacg agcacgaccg tggcgcgctc
    18241 gcgcggcacc atctcgatgc cgttcggacg cgcccaggcg aagctcagcg cgaacatggc
    18301 agccagcgac agcgccaccg cgagatgccg gcgccattgc gactgcttgg gcaccacccg
    18361 gccgaggatg ctggtattgg tgaagcgcat gccggtcttc ttcttgcgca acaccaggaa
    18421 gatgtagccg gcgatcatca gcggaatcag cgccagcacc cagagccgct ccgggttgac
    18481 gaaggtgggt ggccactcga tcacttggtc acccccgccg gtggctggtg cagcatgccg
    18541 gcggtgcgtc ggtaggcgag cacgaagcgg gcgatgtcat gcacccagtc tcggtcggta
    18601 cgcaactgga tgtgcgcggc cccggcccgg cgcagggcca cccggacacg ttcgcgctgg
    18661 gccgcggtgg ccgcgtccat gcgttcgcga gcagcttcgt cggacgtgtt cacgtaccgc
    18721 tcgaagtcgg tctccgggtc gcggatcagc agttcgccga cgtcggcgaa ctcgacctcg
    18781 tgccggtcga ccacctccac cgcgagcacc tgattgcgca ccgcgagttc gcgcatgggc
    18841 cgctcccact cgggtgcgac gttcgcgtcc agctcgaagt ctccaggggt gaggaagtcg
    18901 gacacgacca cccgcatgcc acggcggcgt tcggccctgg ccagccgctg cacaccggcg
    18961 gcaagggtgt acgggccggt ggtgtggtcg gacacgatcg gctcggtgag catccggcga
    19021 agcagtccgt agagcgcggt gcgcccggat tgggccggca gccgcttgat ctcgtccggc
    19081 cgcatgatca gcccaccgaa acggtcgccc atcttctggc tgaggaaccc gatcgtggcg
    19141 atcgcggcga tgcccagatc gcgcttggtc acgccctcag tgccccagtt catggacggg
    19201 gtgacgtcga gcagcgccca gatctccagt tcacggtcgg cgatcgtgtc ccggacgtgc
    19261 ggcaccgtgg tgcgcgccgt gaccgcccag tcgatcttgc ggacgtcgtc ctgccccggc
    19321 acgtaggcgc gcgcgtcgtt cgcctccgat cccgggccgg gcagcaggcc ctggtggtcg
    19381 ccgtgcagga agccctccag gcggcggacg atcgtcagct ccaaccgacg cagcgcggcc
    19441 tcgggcgcga gcttgtgcag cgggatcgtc gcgctcttcg gctcggccag cactgaaccc
    19501 accgcgccga ggtcttcggg cggtggtgcg aagccgggct ggacggacac gctcagctac
    19561 gagcttccgg ggcgtagcgg tgctgggtgt gggcagcctg acgctgctgg ttgtgccaca
    19621 ccggggtggg cggcggcacc atggcgacga tccgctcgac cacctgtgcg gacgtgatgt
    19681 tgtcggccac agcgtcgaag ccgagcacca tgcggtggct catcacgtcg cgggccacgg
    19741 cctggacgtc agtgggcaga acgtagtcac ggccatggat cagggcgagg gcgcgcgccg
    19801 cggcgaccag gccgagggtg gcgcgtggcg agcagccgac ctggatcacc gaggtgagat
    19861 cgggcatgcc gaactcctcc ggcgtccgcg tggccagcac cagccgcacc acgtactcgg
    19921 ccaccaggtt gtgcacgaag acgttggacg cctgctcctg cagcttgagg atcgtctcgg
    19981 gctccagcac ggggttagcc gagggcggcc ggacgctcat ccggcggagg atctcgaact
    20041 cctcgttgcc gcgcgggtag ggcacgtcca ccttcagcag gaagcggtcg cgctgcgcct
    20101 cgggcagcgg gtagacgcct tcggactcga tcgggttctg ggtggcgatc acgatgaacg
    20161 gctgcggcac cttgtaggtg tggccgccga tggacacctg ctgctcggcc atcagctcca
    20221 gcatcgccga ctgcaccttg gccggtgcac ggttgatctc gtcggcgagc acgaagttca
    20281 cgaacaccgg gccgagcgcg atgtcgaact tctcctgccg ggccgagtag at
//
LOCUS       DAOPRQ010000046        20301 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_2235_length_20301_cov_13.409167, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000046 DAOPRQ010000000
VERSION     DAOPRQ010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 20301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20301
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2235_length_20301_cov_13.409167"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1174
                     /locus_tag="PKJ61_07385"
     CDS             <1..1174
                     /locus_tag="PKJ61_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015069460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HOC13685.1"
                     /translation="KDKMARQRLQEAAERAKIELSSAQETSINLPYITAGAEGPLHLD
                     EKLTRAEFQRLTQDLLDRCRAPFNAVIADANTSIGKIDHVLLVGGSTRMPAVVELVKE
                     LTGGKEPNKGVNPDEVVALGASLQAGVLKGEVKDVLLLDVTPLSLGIETKGGVMTKII
                     ERNTTIPTKRSEVFTTAEDSQPSVMIQVYQGERDFARDNKSLGNFELTGLMPAPRGVP
                     QIEVAFDIDANGIVHVHAKDMATGKEQSMTVTGGSALGRDDIDRMVKEAEAHAEEDHK
                     RRQAVEMRNEADALAFRTEKLLADNAETLPEDTKAPVVEALDKLKEALKTDDNDAVKE
                     AMDDLNAKAATMGQAMYAAAQAKQESEAAQASPEGAPASDDDVVDAEIVDEDGDQK"
     gene            1171..1740
                     /gene="grpE"
                     /locus_tag="PKJ61_07390"
     CDS             1171..1740
                     /gene="grpE"
                     /locus_tag="PKJ61_07390"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOC13686.1"
                     /translation="MTDTFGEPIEGQEPDEGLEPVEGAGAVPAPDEGLKPDAEPATQA
                     AIAELQELVAERTDDLQRLHAEYANYKKRVDRDRALARQGGVEAVVLDLLPVLDSVEA
                     AREHDELVGGFKLVADELEKATAKYGLETFGEVGEEFDPQLHEALMQMPFPGEAEITV
                     PTISAVMQRGVKLNERLLRPARVGVANPE"
     gene            1765..2913
                     /gene="dnaJ"
                     /locus_tag="PKJ61_07395"
     CDS             1765..2913
                     /gene="dnaJ"
                     /locus_tag="PKJ61_07395"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016668217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HOC13687.1"
                     /translation="MSTKDWIEKDYYKVLGVSSSAKPEEIKKAFRKLAREYHPDQNKQ
                     NPDAEKKFKEISEANSVLSDAAKRKEYDEARRLFGGGGFRFPTGGQGAGAGPSMEDLF
                     RNATGDSGISDLFGNLFGGATASRRSRGPRRGADIEGEVTIDFADALAGAMVTMQTTS
                     EAACETCHGTGARPGTQPTVCRACQGSGMQTSSSSSVFAVSEPCRECRGRGMVVEDPC
                     PTCHGSGRGHSTRSMQVRIPAGVTDGQRIRIKGKGGAGENSGAAGDLYVLVHVRPHPI
                     FGRKGDHLTITVPITFTEAALGAEIEVPALDGSKVRVRIPAGTPTGRTFRVRGRGVRG
                     GAADGDLLVTVEVQVPKHLNEEARAALAEFQTKAALPNPREHLLKVQR"
     gene            2910..3332
                     /locus_tag="PKJ61_07400"
     CDS             2910..3332
                     /locus_tag="PKJ61_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOC13688.1"
                     /translation="MTSDFLPDRLDADAPIFVISVAAQLAEMHPQTLRGYDRLGLVVP
                     GRTRGRGRRYSLRDIAKLRHIQQLSQNEGINLEGIRRILALEAEVESLHVQLARLASV
                     VHELQLLPDAARLFTAEAGGSVHIGRTRVRQAREIAAR"
     gene            complement(3341..4219)
                     /locus_tag="PKJ61_07405"
     CDS             complement(3341..4219)
                     /locus_tag="PKJ61_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009076473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator ArgP"
                     /protein_id="HOC13689.1"
                     /translation="MELPLELLRTIDVIVSTGSLEDAAAELSITPSAVSQRLKALETR
                     VGRVLVVRSKPVRATADGQVLIRLARQLATLEHDTLASLGLGEDGPVEVPLAVNADSL
                     ATWFLPALAPLATDIVFDLHRDDQDYTVGLLESGTVTAAVTSQAQPVAGCSVRPLGAM
                     RYLPVAAPGFYQRWFEGTPAVEAFGRAPAIDFDRRDDLQTRYLITRGVDPALPPRHHV
                     PASHDFASAVLLGMGWALIPEQQSRPALADGRLVGLGGAPVDVQLYWQRWKLASELLT
                     RIEDAVVSAARSALRQ"
     gene            4290..4955
                     /locus_tag="PKJ61_07410"
     CDS             4290..4955
                     /locus_tag="PKJ61_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009076471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE/ArgO family amino acid transporter"
                     /protein_id="HOC13690.1"
                     /translation="MSHLLAGLGLGFSLIVAIGAQNLFVLRQGIRRERTVLVATICAV
                     SDVILIVVGVSGIGAATTALPWLVDVVRWAGAVFLVGYGLLAARRALRPDGSALVLTD
                     SNEPGTTSSGPATTVTLVAARPRTAASVALTALALTWLNPHVYLDTVFLLGSVAATHG
                     DQRWVFAAGAMVASFVWFFGLAFGARLLGRWLATVRAWQVLDGVIAVVMIGLGVSLVL
                     PGA"
     gene            4992..6413
                     /locus_tag="PKJ61_07415"
     CDS             4992..6413
                     /locus_tag="PKJ61_07415"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOC13691.1"
                     /translation="MSVSVLRLANWSIRARVISAVVALTGIALALSGLIVTTRGHALT
                     EQRVAADLQLGATEFQALATEGVDPRTGVGFVDAESLTRVAVQRSVLAANEGVFGVLD
                     GKVRWAAQEGATFRLDQDDEFTAAVLPLTNLATISQGRLVTSQRDYRYIVVPVSFANG
                     TPGGALVRAVDFGAEESLLGEVWRSYILVALGSLLLVGLLIWILVGRLLEPLAWMRRT
                     AEKITDADLSERIPVRGTDDLSALGETMNSMLDRLEGAVGAQRELLDDVGHELRTPLT
                     IIRGHLELMDAADPADVVATKELLLDETDRMGRLVDDLLALARAERPELLRPVRTDVA
                     RLTDETLAKASGLGNRHWVLDELADVEFVLDPQRVAQAWLQLAANAVRYSTDASTVGM
                     GSQLRRGELRLWVRDEGVGISPEDIARVTNRFERGVGGGTGLGLAIVNAIARAHGGRI
                     EIESERGVGSRISIVLPAREESE"
     gene            6410..7075
                     /locus_tag="PKJ61_07420"
     CDS             6410..7075
                     /locus_tag="PKJ61_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOC13692.1"
                     /translation="MSQILIVEDEPRISAFIAKGLRAAGFATLVEPSGVAAVQLALVE
                     PIDLILLDVGLPDIDGFEVLSRIRGQGVSVPVIMLTARTSVADRVAGLQSGADDYVPK
                     PFAFEELLARIHLRLRAEGGSDADSATVLSHRDLSLDLRTRRASVGGRSVDLTGREFT
                     LAEVFLRNPGQVLSREQLLSGAWGYDFDPGSNVVDVYVRYLRRKLGADYFETVRGMGY
                     RLT"
     gene            7186..7452
                     /locus_tag="PKJ61_07425"
     CDS             7186..7452
                     /locus_tag="PKJ61_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13693.1"
                     /translation="MLLGETVALALSEPDADGDADGVLLGSLEAVLNGTTTAGRASIG
                     AGPLPWSAKAPSPTEVSPIAPDTAQKVERCDIGTFGLLSLLVQG"
     gene            complement(7486..8685)
                     /locus_tag="PKJ61_07430"
     CDS             complement(7486..8685)
                     /locus_tag="PKJ61_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13694.1"
                     /translation="MSDLDRAAALLQSPDAGGLLLAALTGDPSADVPGFTATLANWQY
                     RPGAEVTAGYSVSYADPSGRPVQEDLFVTTADVAAPATLSRDHLSFAVWRHPGDPLLP
                     GLAAACDPATVMSWLQVTPNHFDLTLLAYRPLRRAVLRATADGSETYLKVLRPERADR
                     LAVRQNLLADAGLTPALLARPAPGVLLTPRASGRSLAHVLATHDDPELPAAADLIGLL
                     DALPNQLCDLPRRPAWSDRLDFHTATAVDRLPAHTERLHALQRKLQGVLDTAPVGPLV
                     PTHGDFYEANLFVDSPRLMLIDLDASGPGRREDDLACLLAHLVVLPGLSPEHYRAVPI
                     VLERWTDEFAALVAPAALFARVGAVVLSLIAGADGNQAEHRLELAEAWAERARGEHGR
                     RPEMREP"
     assembly_gap    8762..8861
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8862..9218
                     /locus_tag="PKJ61_07435"
     CDS             <8862..9218
                     /locus_tag="PKJ61_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13695.1"
                     /translation="VFGVLGVLGVLAVLGVFGVFGVSEVLTVTLESLAVADGSALAEG
                     ELVADGLAEVLPGVASAVTDGVGLGVTVSAVARVAPPSTLMPTAPVTAQRAVDREIFM
                     VCPFSVIDWFEFTRRW"
     gene            9395..11086
                     /locus_tag="PKJ61_07440"
     CDS             9395..11086
                     /locus_tag="PKJ61_07440"
                     /inference="COORDINATES: protein motif:HMM:NF025182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4185 domain-containing protein"
                     /protein_id="HOC13696.1"
                     /translation="MGVPVDPERPLIGPRSMLLIRQGLRRWLAAVVLTGNRLDHYQRR
                     LDRPDRPWVRLGAITELATGAGALSSRHGVLIVQVPQADGAHVHQLLDATWQPAVAIS
                     QPVAQTEPIPDDLSDSAVRSRHARAICVVGDRALADEAGSIFGYRRVDGRWERDSCLR
                     LADAEPFIADGPESVKLAQVSGDADATPTPWGVREPTLSRSLATAGIVGTDLGVRVEH
                     EGRSFLLFGDTHWRGRPWLRTRDAVAEITNDGPRPGLPGVRFHGSPLKLTGAPLLGRD
                     RVTMREYDVPLDGYSQVGQLFVFFTSNHGRDGRVMGRSILTVADSQLTIDPAARRRPL
                     RFRALGTFSAHHFINVSTQLRPAATVPGCIGDGDVLLVWGTGPYRAGLPRLALLDQAA
                     LAAIATGGSPAEAGVRYWNRAGWSQHEPEAEPLFEPAALGELSVRWVPEVSRYLMLTA
                     SGPEDPIGHAITLRTAVHPWGPWSPRRRLLDWVATGMAHHDPHTRFIKAATDGSDPVG
                     DQVFAAQARGTGAAYAPYFFDAAVDGEELLLRYTLSTWNPYQVVLMAHRLPIAAL"
     gene            11196..12380
                     /locus_tag="PKJ61_07445"
     CDS             11196..12380
                     /locus_tag="PKJ61_07445"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HOC13697.1"
                     /translation="MSVRTESVRIWAWRVLLVLPPLLAAIHAGGTQIPGASLLPWHPG
                     MIDLEVYRRTGELVLSGGDIFNAEGLPWIYPGFAALLTVPFAVVPFELAAVAWLALSA
                     AALAAIIYRLGYTGWQLSALTTVCILFVHPVRETLGFGQLGIFLVAAAVLDSMPGPRV
                     FKRRILPEGWLVGVATAVKLTPAVVAAYNFFAGRRKPGLVAFASFLAATALGFVLLPQ
                     ASFAYWAKLASGDSGLNSGIPYATNQSVLGMWNRLTGEPGRVGLLLSVLVVFLGVWAA
                     VLMHRRGQVALAICLAGLTSLLASPISWSHHYVWVVPLGLVLWQARELPGYLRWPGLA
                     YVLWVVVSPFMWLPRGDDVELSYAPWMQFVDNIGIVVGVLLLAGSAATALGPWGRKQV
                     LP"
     gene            12377..13258
                     /locus_tag="PKJ61_07450"
     CDS             12377..13258
                     /locus_tag="PKJ61_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13698.1"
                     /translation="MRSTRVWFIVAAAFAFASVVMGTVVCATESGAACPNWPGCYPDQ
                     FAPQAQLNPMIEFLHRVIAALTGPVVLIAAILARKVADPRPRWLAWIGLAGTISAGVF
                     GMLIVRIGIPWWLGVVDLASALIATVALIVAALLMRPGAAWTPGRTARLAWAAVGTLA
                     VMHLSALAVAGDNAFTRCISWPLGVFETDHWPVLQWLRIVLALVAAVLIVLLVADGVR
                     TPRLRLPALASGVLLVLELMLVPVLLGGYDDLVLVTAYAVTAAALFGSVSLLAGRASV
                     RLDVPVGAADASALTVA"
     gene            13468..16044
                     /gene="clpB"
                     /locus_tag="PKJ61_07455"
     CDS             13468..16044
                     /gene="clpB"
                     /locus_tag="PKJ61_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002529320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HOC13699.1"
                     /translation="MDTEKLTARSRDAVSAAVRTALTNGNPNAEPVHLLHALLLVPEN
                     AVAPLLASIGVDPTVVDAAARGAITKLPSAQGSSVSQPAMSGGLARVLADAETRAQQL
                     GDQFVATEHLLLALSAVQTDAKKILNDLGATTDKLAKAFNDARGSKRVTSAESEGGES
                     ALDKYSVDLTERAREGKLDPVIGRDSEIRRVVQVLARRTKNNPVLIGEPGVGKTAVVE
                     GLAQRVVAGDVPDSLKGRRVVSLDLASMVAGAKYRGEFEERLKAVLNEIRDAEGQVIT
                     FIDELHTVVGAGATGDSSMDAGNMLKPMLARGELRMIGATTLDEYRERIEKDPALERR
                     FQQVFVGEPSIEDTIAILRGLRQRYEAHHKVRITDSALVAAATLSGRYITSRQLPDKA
                     IDLIDEAASRLRMEIDSSPEEIDQLRRDIDRMTMQAFALEKEDDPGSKDRYARLTAAL
                     ADAKEELRGLEARWEAEKSGLNRVGEVKESIDKLRIEAERAQREGDLTKASEILYGRI
                     PALEAELESATEAEQQTKQMVSEEVSATDIAEVVSAWTGVPVGKMLQGESEKLLSMED
                     RLGERLIGQRKAVTAVADAVRRSRAGISDPNRPTGSFLFLGPTGVGKTELAKSLAEFL
                     FDDEHAMVRIDMSEYGEKHTVSRLVGAPPGYIGYEEGGQLTEAVRRRPYSVVLLDEVE
                     KAHPEVFNILLQVLDDGRLTDGQGRTVDFRNVILIMTSNLGSQFMADPKLHADTKQEA
                     VMNVVRQAFKPEFLNRLDEIVIFDALGIEELSRIVDIQLRRLNQRLSDRRIEIEVSDA
                     GKEWLALTGFDPVYGARPLKRLVQTTIEDALARRVLSGEVSDGQTVRFELNEDGSGLV
                     LS"
     gene            16160..16969
                     /locus_tag="PKJ61_07460"
     CDS             16160..16969
                     /locus_tag="PKJ61_07460"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOC13700.1"
                     /translation="MRSRHTARDEVTYKRRHVGKVEYFTSRYGRGDASMVVYRFARLG
                     ERSGPDFVLVHGIGVSARSYGPTAVELAQHGDVYLIDLPGYGRSPRPGVDLSIAGHAQ
                     VLGDFLTDRELDHPVVVGHSMGSQVVVELAAAHPEQVDHIALIAPVIVPSARKLPKLL
                     RLFLQDVVKEPPQVGLMAVYDYLFRAGVPYMIEQTPHLFGARVEETAARVEAKTLVIC
                     GEDDPLVPLDWGRELADDFRHGWFATVPGPHATMFAAPERIAKLLDEHAHR"
     gene            16956..17675
                     /locus_tag="PKJ61_07465"
     CDS             16956..17675
                     /locus_tag="PKJ61_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13701.1"
                     /translation="MPIGSPGADAAARFKLAVRRARYWARDYFYVTHRQLRGPLERAV
                     PTAFASGDRAPVVLLAGVLEPWTLLLPAAERLNRDGHPVQVVPELAYNLATLDAAAEL
                     AMNAVTAKELTGVILVAHSKGGLVGKRMLELDADGRLRGLIAIATPFGGSSLARLVPS
                     RTIRALGPEDPAIQALAKRSDLNPLITSIYPSFDPHIPDGSHLDGAVNIEVAAIGHFR
                     VLKDAEVLDAVAAAAERLSQA"
     gene            complement(17676..17963)
                     /locus_tag="PKJ61_07470"
     CDS             complement(17676..17963)
                     /locus_tag="PKJ61_07470"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOC13702.1"
                     /translation="MPLTDRMLDEVAGSLGQKVRQLRERRHWTQEELAHKVGMSRNQI
                     QNIERSRNNTRDPKTGRLGRGNPRLDTIYLLADVLGVKPAVLIDPELELPE"
     gene            17962..18168
                     /locus_tag="PKJ61_07475"
     CDS             17962..18168
                     /locus_tag="PKJ61_07475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13703.1"
                     /translation="MAPTLSAALVRSQPNRLGIAEQRHYALPGGALLVAMYRLHNLPL
                     TVLGTPSFSGVDWRDIVAESSRRW"
     gene            18214..18789
                     /locus_tag="PKJ61_07480"
     CDS             18214..18789
                     /locus_tag="PKJ61_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13704.1"
                     /translation="MEEEVDALLRHSRSIRSFVTADGLRRRREDNPSSAYQEAAENLL
                     LKAWRELVDDPGRAVSYVERALRLPFDPNEDSAPAAYAAHMLLFNLVVDALEDSEEGE
                     EEWLAAAIQVLNSADPRASSELRDVLEVVAQDYHITSSERRKLAAAVKSAPPRQSLFD
                     LQLPPDELCDHIVAVLHACEAYETALDELFA"
     gene            18996..19811
                     /locus_tag="PKJ61_07485"
     CDS             18996..19811
                     /locus_tag="PKJ61_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002516252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HOC13705.1"
                     /translation="MVSNRVVPAPDDRRAFMVVAGNTEQSWVDPANPLRLEFEYVQRI
                     AEILDLTVLSRPAAERVRIVHVGGGGLTLPRYVAARRPHTAQIVLEPDEELTAEVRAR
                     LPLPRNSGIKIRAHDGRSGVAQLPDAYADALILDAFAAASVPAELATVEFFTDVRRAL
                     RPGGLLIMNLTDQAPFGWSKRCLAALFATQPQLVASAEVPVWKGRRFGNLVAVAGPDL
                     PETDFSRALARAPFPYRLLAGRELVRFIGAATPFTELDAEPSPPPAWSRTWFS"
     gene            19874..>20301
                     /locus_tag="PKJ61_07490"
     CDS             19874..>20301
                     /locus_tag="PKJ61_07490"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HOC13706.1"
                     /translation="MTARDELIELIKSLAVVRGKVTLASGKEADYYVDLRRVTLDGVA
                     APLVGEVMTELVADWDFVAAGGLTLGADPVAVAMLHHAAHTGGRLDAFVVRKESKAHG
                     LQRRIEGAEVAGRRVLVVEDTSTTGGSVLTAVDAVREAGG"
BASE COUNT         3082 a         6700 c         7096 g         3323 t
ORIGIN      
        1 caaggacaag atggcccgcc agcgcctcca ggaggccgcc gagcgcgcca agatcgagct
       61 gtcgtccgcg caggagacct cgatcaacct gccgtacatc acggccggcg ccgagggccc
      121 gctgcacctg gacgagaagc tcacccgagc cgagttccag cggctcaccc aggacctgct
      181 ggatcgctgc cgggcgccgt tcaacgcggt gatcgcggac gcgaacacgt ccatcggcaa
      241 gatcgatcac gtgctgctgg tcggcggttc gacccgcatg cccgcggtgg tcgaactggt
      301 caaggagctc accggtggca aggagcccaa caagggcgtc aaccccgatg aggtcgtggc
      361 tctgggcgcg tccctgcagg ccggtgtgct gaagggcgag gtgaaggacg tgctgctgct
      421 cgacgtcacc ccgctgagcc tcggcatcga gaccaagggt ggcgtgatga ccaagatcat
      481 cgagcgcaac accacgatcc cgaccaagcg ttccgaggtg ttcaccacgg ccgaggacag
      541 ccagccgtcg gtgatgatcc aggtgtacca gggcgagcgt gacttcgccc gggacaacaa
      601 gagccttggc aacttcgagc tgaccggcct gatgccggcg ccgcgcggcg tgccgcagat
      661 cgaggtcgcc ttcgacatcg acgccaacgg catcgtccac gtccacgcca aggacatggc
      721 cactggcaag gagcagtcca tgactgtcac cggcggttcg gcccttggcc gcgacgacat
      781 cgaccgtatg gtcaaggaag ccgaggcgca cgctgaggag gaccacaagc gtcgccaggc
      841 cgtcgagatg cgcaacgagg cggacgcgct cgcgttccgc accgagaagc tgctcgccga
      901 caacgccgag acccttcccg aggacaccaa ggccccggtc gtcgaggccc tcgacaagct
      961 gaaggaagcc ctcaagaccg acgacaacga cgcggtcaag gaagccatgg acgacctgaa
     1021 cgccaaggcc gccaccatgg gccaggcgat gtacgccgcc gcccaggcca agcaggagtc
     1081 cgaggccgcc caggcatcgc ccgagggcgc acccgcgtcc gatgacgacg tggtggacgc
     1141 cgagatcgtg gacgaggacg gcgatcagaa gtgaccgata cgttcggaga gccgatcgaa
     1201 gggcaggagc ctgacgaagg gctcgagccg gtcgagggcg caggagcggt ccctgcgccg
     1261 gacgaaggcc tgaagccgga cgccgagccg gcaactcaag cggcgatcgc ggagctgcag
     1321 gaactcgtgg ctgagcgcac cgacgacctg cagcgcctgc acgccgagta cgccaactac
     1381 aagaagcgtg tggaccgcga ccgtgcactg gcccggcagg gcggggtcga ggcggtagtg
     1441 ctcgacctgc tgcccgtcct ggacagcgtg gaagcggccc gcgagcacga cgagctggtc
     1501 ggcggcttca agctggtcgc cgacgagctg gagaaggcca cggccaagta cggcctggag
     1561 accttcggcg aggttggtga ggagttcgac ccgcagctgc acgaggctct gatgcagatg
     1621 ccgttccccg gcgaggccga aatcaccgtc ccgaccatct cggcggtgat gcagcgtggc
     1681 gtgaagctca acgagcgcct gctgcgtccg gcgcgagtgg gcgtggccaa ccccgagtga
     1741 cgtgcgacta ggagaggagg cgcgatgagt accaaggact ggatcgagaa ggactattac
     1801 aaggtgctcg gcgtctcctc gagcgcgaag cccgaggaga tcaagaaggc gttccgcaag
     1861 ctggccaggg agtaccaccc cgaccagaac aagcagaacc cggacgccga gaagaagttt
     1921 aaggagatct ccgaggcgaa ctcggtgctc tccgacgctg ccaagcgcaa ggagtacgac
     1981 gaggcccggc ggctgttcgg cggtggggga ttccggttcc ccaccggcgg gcagggcgcc
     2041 ggcgccggtc cgtcgatgga agacctgttc cgcaacgcca ccggcgattc gggcatctcc
     2101 gacctgttcg gcaacctctt cggcggtgcc acggccagcc gacgtagtcg tggaccgcgc
     2161 cgcggtgccg acatcgaggg tgaggtcacg atcgacttcg cggacgcctt ggcgggcgcg
     2221 atggtgacca tgcagaccac gtcggaggcg gcgtgcgaga cctgtcacgg cacgggtgcc
     2281 cggccgggga cgcagccgac ggtgtgtcgc gcctgccagg gcagcggcat gcagacctcc
     2341 tcatcgagca gcgtgttcgc cgtcagtgag ccctgtcgcg aatgccgtgg ccggggcatg
     2401 gtggtcgagg acccgtgccc gacctgccac ggcagcggac gtggtcactc cacccggtcg
     2461 atgcaggtgc ggattccggc cggcgtgacc gacggccagc gcatccggat caagggcaag
     2521 ggtggcgccg gcgagaacag cggcgcggcc ggcgacctgt acgtgctggt gcacgtgcgg
     2581 ccgcacccga tcttcggccg caagggcgat catctgacga tcaccgtccc gatcacgttc
     2641 accgaagctg cgctgggggc cgaaatcgag gtgccggcgc tggacggttc gaaggtgcga
     2701 gtgcggattc cggcgggcac gcctaccggg cgcacgttcc gcgtccgcgg tcgtggggtg
     2761 cggggcgggg ctgcggatgg cgacctgctg gtgaccgtcg aagtgcaggt gccgaagcat
     2821 ctgaacgagg aggcgagggc ggcgctggct gagttccaga ccaaggctgc gctgccgaac
     2881 ccgcgggagc acctgctgaa ggtgcagcga tgacctccga tttcctgccc gaccggctgg
     2941 acgccgacgc cccgatcttc gtgatctcgg tggccgcgca gttggcggag atgcatccgc
     3001 agactctgcg tggctatgac cggttgggtc tcgtcgtgcc aggacggacc cgcggtcggg
     3061 gccggcgcta ttcgctgcgc gacatcgcga agctgcggca catccagcag ctctcccaga
     3121 acgagggcat caacctcgaa ggcatccggc gcatcctggc gctggaagcc gaggtggagt
     3181 cactgcacgt ccagctcgcc cgcctggctt cggtggttca cgaactgcag ctgctgccgg
     3241 acgcagcgcg cctgttcacc gccgaagctg gcggctccgt gcacatcggg cgcacccgcg
     3301 tccgccaggc ccgagagatc gccgcccgct gaacggtccc tcactggcgt agggctgagc
     3361 gggctgcgct caccacggcg tcctcgattc gcgtgagcaa ctcggacgcg agcttccaac
     3421 gctgccagta gagctggacg tccacagggg cgccgcccaa cccgacgagc cggccgtccg
     3481 ccagcgccgg tcgggactgt tgctcgggga tcagtgccca gcccatgccg agcaacactg
     3541 cggacgcgaa gtcgtgcgaa gccggcacgt ggtggcgcgg cggcaaagcc gggtcgactc
     3601 cgcgggtgat caggtagcgg gtctgcagat cgtcacgccg gtcgaaatcg atcgcggggg
     3661 cgcgtccgaa ggcctcgaca gctggggttc cttcgaacca acgctggtag aacccgggtg
     3721 ccgcgaccgg caggtagcgc atcgccccta gtggacgtac cgaacatccg gccaccggct
     3781 gcgcctgaga ggtcacggcc gcggtcaccg tcccggattc aagcaggccc acggtgtagt
     3841 cctggtcgtc gcggtgcagg tcgaacacga tgtcggtcgc cagcggtgcg agcgcgggca
     3901 ggaaccaggt cgcgaggctg tccgcgttga ccgccagcgg cacttcgacc gggccatcct
     3961 cgccgaggcc caggctcgcg agggtgtcgt gctcaagggt ggccagttgg cgggccagcc
     4021 ggatcagcac ctgtccgtcc gcggtggcgc ggacgggctt ggaccgcacc accagcaccc
     4081 ggcccacccg ggtctcgagt gccttcaggc gttgcgatac cgcggacggg gtgatgctca
     4141 actcggctgc ggcgtcttcc agcgagccgg tcgagacgat gacgtcaatc gttcgcagca
     4201 attccaacgg cagttccaca taagcgatgc taatgcaagg caagcttctt tagctgtact
     4261 tacccgagtt ggctggttag cctcgaagca tgtcccacct gctggccggc ctcggcctcg
     4321 gcttctcgct catcgttgcc atcggtgcac agaacctgtt cgtgcttcgc cagggcatcc
     4381 gacgcgagcg gacggtgctg gtggccacga tctgcgcggt gtcggacgtg atcctgatcg
     4441 tggtcggggt ttccggcatt ggcgcggcca ccactgcgct tccctggctc gtcgacgtgg
     4501 tgcgctgggc aggcgcggtg ttcctcgtcg gctacggcct gctggcggcc aggcgggcgc
     4561 tgcgtccgga cggatcggcg ctggtgctga ccgactccaa cgagccgggg accacctcgt
     4621 ccggcccggc aacgacggtc acgctggtcg cggcgcgccc ccgtaccgcg gcctcggtgg
     4681 cgctgaccgc cctcgcgctc acctggctca acccgcacgt gtacctcgac accgttttcc
     4741 tgctcggctc ggtcgccgcc acccacggcg accagcgctg ggtgttcgcc gccggcgcca
     4801 tggtggccag tttcgtctgg ttcttcgggc ttgccttcgg cgcccggctg ctcggacgct
     4861 ggctggcgac cgtccgcgcg tggcaggtgc tggacggcgt gatcgcggtg gtgatgatcg
     4921 gcctgggcgt ctcgctggtg ctgcccgggg cttagctcgg ctttgtcgta cgcaccgggc
     4981 aaactagggc gatgagcgtc tccgtcctgc gcctggccaa ctggagtatc cgtgcccggg
     5041 tgatctccgc cgtggtggcg ctgaccggaa ttgcgctggc cctctccggc ctgatcgtga
     5101 ccacccgcgg ccatgcgctc accgagcagc gggtggccgc cgacctgcaa ttgggggcga
     5161 ccgagttcca ggcactcgcc acagagggcg tcgacccgcg cacaggcgtc ggcttcgtcg
     5221 acgctgagag cctcacccgc gtcgccgtcc agcgctcagt gctcgcagcg aacgagggtg
     5281 tcttcggtgt tctcgacggc aaagtgcggt gggccgccca agagggcgcg acgttccggc
     5341 tcgaccagga tgacgagttc accgccgcgg ttctgccgct gaccaacctc gccacgatca
     5401 gccagggcag gctcgtcacc agccagcgtg actatcgcta catcgtggtt ccggtcagct
     5461 tcgccaacgg cacgcccggc ggtgcgctgg tgcgggcggt cgacttcggc gcggaggaga
     5521 gcctgctcgg cgaggtctgg cgcagctaca ttctggtcgc actcggctcg ttgctgctgg
     5581 tcgggctgct gatctggatc ctggttggac gcctactcga accactcgcc tggatgcgcc
     5641 ggaccgcgga aaagatcacc gacgccgacc tgagcgagcg catccccgtc cgcggaaccg
     5701 atgatctgtc cgcactcggc gagacgatga acagcatgct cgaccggctc gaaggtgccg
     5761 tcggcgcaca gcgcgagctg ctcgacgatg tcggccacga gttgcgcacg ccgctgacga
     5821 tcatccgcgg gcacctggag ttgatggacg cagccgaccc ggccgatgtg gtcgcgacca
     5881 aggagttgct gctcgacgag accgaccgca tgggccgcct ggtcgacgac ctgctggcac
     5941 tcgcccgggc ggagcgtccg gagttgcttc gcccagtgcg gaccgacgtc gcccggctca
     6001 ccgacgagac gctcgcgaag gcgtccgggc tgggcaaccg gcactgggtg ctggacgagc
     6061 ttgcggacgt cgagtttgtg cttgacccgc agcgcgtggc gcaggcgtgg ctgcaactgg
     6121 cggccaacgc cgtccgttac tcgacggatg cctcgaccgt cggcatgggt tcgcagctgc
     6181 gtcgcggtga gttgcggctg tgggttcgcg acgagggcgt cgggatcagc cccgaggaca
     6241 tcgcccgggt cacgaaccgc ttcgagcgcg gcgtgggtgg cggcaccggg ctcgggcttg
     6301 cgatcgtgaa tgctatcgcc agagcgcatg ggggccggat cgagatcgag tccgagcgtg
     6361 gcgtcggcag ccggatcagt atcgtgctgc cggccagaga ggagtctgag tgagccagat
     6421 cctgatcgtc gaggatgaac cgcggatcag tgccttcatc gcaaagggct tgcgggcggc
     6481 cggatttgcg accttggtcg agccttcggg ggtggccgcg gtgcagttgg cgttggtcga
     6541 gccgatcgac ctcatcctgc tggacgtcgg cctgcccgac atcgacggct tcgaggtgct
     6601 gtcccggatt cgcgggcagg gggtcagcgt tccggtgatc atgctgaccg cgcgcacctc
     6661 ggtggccgac cgggtcgccg gactgcagtc gggcgcggac gactatgtgc ccaagccgtt
     6721 tgctttcgag gaattgttgg cgcggatcca cctgcggctg cgcgctgagg gcgggtccga
     6781 tgcggactcg gcgaccgtgt tgtcgcatcg cgacctctcg ctcgatctgc gcactcgccg
     6841 ggcgagcgtc ggcgggcgtt cggtggatct gaccggacgt gagttcaccc tggccgaggt
     6901 cttcctgcgc aaccccggcc aggtgctcag ccgtgagcag ctactttcgg gcgcttgggg
     6961 ctacgacttc gacccgggct cgaacgtggt ggacgtctat gtccgctacc tacgccgcaa
     7021 gctgggcgcc gactatttcg agaccgtccg cgggatgggc taccggctca cctgagtcga
     7081 ggtcactggg cttggatccg ggcctagtcc ggcgtctcgg cgctcggcgg gctgaccggc
     7141 gaaacgctgg acttcgcgct cggcttggcg gacggcgtct tgccagtgct gcttggggaa
     7201 acggttgcgc tggcgctctc cgagccggac gcggacggtg acgccgacgg cgtgctgctc
     7261 ggatcgctcg aggcggtgct caatggcacg acgaccgcgg gtcgggcttc gatcggcgcc
     7321 gggccgctgc cctggagcgc gaaggcgccg agtccgacag aggtgagtcc gatcgcgccg
     7381 gacaccgccc agaaggtgga acgctgtgac attggcactt tcggcctcct ttcgttgctg
     7441 gttcaaggct aggtgagggt gtctgcccgg gccagagcgg acggattagg gttctctcat
     7501 ctcgggccgc cgcccgtgct cgccgcgcgc acgctccgcc caggcttcgg ccaactcgag
     7561 ccggtgctcc gcctggttcc cgtccgcgcc ggcgatcagg ctgagcacca ccgcacccac
     7621 ccgcgcgaac agcgccgcgg gcgcgaccag ggccgcgaac tcgtccgtcc accgctcaag
     7681 gacgatcggc accgcccgat agtgctccgg cgacaggccc ggcagcacga ccaggtgcgc
     7741 cagtagacag gccaggtcgt cttcgcggcg cccagggccg ctggcgtcga ggtcgatcag
     7801 catgagccgt ggtgagtcga cgaaaaggtt cgcttcgtag aaatcgccat gggtagggac
     7861 gagcggcccg accggcgcag tgtcgagcac accctggagc ttccgctgca aggcgtgcag
     7921 acgctcggtg tgcgcgggca accgatccac cgcggttgcg gtatggaaat cgaggcggtc
     7981 cgaccacgct ggtcggcgcg gcaggtcgca gagttggttg gggagcgcgt cgagcaggcc
     8041 gatcaggtcg gctgcggccg gcaactcggg atcatcatgc gtagcgagga cgtgagccag
     8101 cgagcgtccg gatgcccgcg gggtgagcag cacgcccggg gccggccggg ccagcagcgc
     8161 aggggtcagc ccggcgtcag ccagcaggtt ctggcggacg gcgagccggt cggcgcgctc
     8221 cgggcgcagc accttgagat aggtctcgct gccgtccgcc gtggcgcgaa gcactgcccg
     8281 gcgcagcgga cggtaagcca ggagggtgag atcgaagtgg ttcggcgtca cctgtagcca
     8341 ggacatcacc gtcgcggggt cgcaggccgc tgccaacccg ggaagcagtg ggtcgccggg
     8401 atgccgccag accgcaaagc tcaagtggtc ccggctgagg gtggccggcg cagccacgtc
     8461 cgcggtggtg acgaagaggt cctcctgcac gggacgtccg ctcgggtcgg cgtagctgac
     8521 cgagtacccg gcggtgacct cagccccggg ccggtactgc cagttggcca gcgtggcggt
     8581 gaaccctggc acgtcggcgg acgggtcgcc ggtcagcgcg gcgagcaaca gcccccctgc
     8641 gtccggggac tgcaggaggg ctgcggctcg gtcgagatca ctcatgtagt cagtctgccg
     8701 aacgggcgct cggggccgac tgcggcgagg ccggggtgtt cggggtgttg ggggtgttcg
     8761 gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngtgttcggc gtgttgggcg
     8881 tgttgggagt gttggcggtg ttgggagtgt tcggcgtgtt cggtgtcagt gaggtgttga
     8941 cggtgacgct ggaatcgctg gcagtcgcgg acgggtcggc gctggccgag ggtgagttgg
     9001 tggccgacgg gctcgccgaa gtgctgcccg gcgtggcttc agccgtgacc gacggggtcg
     9061 gactcggggt gacggtctca gccgtcgcca gggtcgcgcc gcccagcacg ctgatgccga
     9121 cggcgccggt cactgcccag agagctgtgg atcgggagat cttcatggtg tgtcccttct
     9181 cggtgattga ttggttcgag ttcacccgac gctggtgaaa ccactgggag agccccgtga
     9241 gagtcctcta atgcgggggt tcgtcggccg catccgtcca cgaggccctc ggtcccgcac
     9301 agggtggacg gttccgcgcc gtctgagcgc gaaagcgtcc accttgttgg tgggtgaacg
     9361 ctgggccggg tcgctgcgcg actagggtgc aggcgtgggc gtgccagtcg atccggagcg
     9421 gccgttgatc ggtccgcgtt cgatgctgct gattcggcaa gggctgcgcc gctggttggc
     9481 ggcggtcgtg ctcaccggga atcggctcga ccactaccag cgtcgcctgg atcgtcccga
     9541 ccggccgtgg gtccggctcg gtgcgatcac tgagttggcg accggggccg gagcgctgtc
     9601 gagccgccac ggggtgctca tcgtgcaggt gccgcaagcc gatggcgcgc acgtccatca
     9661 actgctggac gcgacttggc agccagctgt cgcgatctcg cagccagttg cgcagacgga
     9721 gccgattccc gacgatctga gcgattcagc cgtgagatcg cgacacgccc gcgcgatctg
     9781 cgtcgtcggc gaccgggcgt tggcagatga ggcgggctcg atcttcggct accgccgggt
     9841 cgacggacgc tgggagcgcg actcctgcct tcgcctggct gacgccgagc cgttcattgc
     9901 ggacgggcct gagtcggtga agctcgctca ggtttccggc gacgccgacg cgaccccgac
     9961 gccgtggggc gtgcgcgaac cgaccctgag ccgctcactc gccaccgccg gcatcgtcgg
    10021 caccgacctt ggtgtgcggg tcgagcacga aggccgaagc ttcctgctct tcggcgacac
    10081 ccattggcgc ggacggccat ggttgcgcac gcgggatgcg gtggcggaaa tcaccaatga
    10141 cggcccacgg ccgggattgc ccggcgtccg cttccacggc tcaccactca agttgaccgg
    10201 tgcgccgctg ctaggacgcg accgcgtcac catgcgcgaa tacgatgtgc cattggacgg
    10261 atactcccag gttggccagc tcttcgtctt tttcaccagc aatcatggcc gtgacggacg
    10321 cgtgatggga cggtcgatac tgacggtggc cgactcgcag ctgaccattg atccggcagc
    10381 gcgaaggcgt ccgctcaggt tccgcgcgct cggcacgttc agtgcgcacc atttcatcaa
    10441 tgtctcgacg cagctgcgtc cggctgccac ggtgccaggg tgcatcggcg acggggacgt
    10501 gctgctcgtc tggggcaccg ggccctaccg cgccggactg ccgcggttag ccctgctcga
    10561 ccaggcggct ctcgccgcga tcgcgacggg cggcagcccg gccgaggctg gcgtccgcta
    10621 ctggaaccgc gcgggctggt cacaacacga gcctgaagcc gaaccgctgt tcgagccggc
    10681 tgcgctgggg gagttgtccg tccggtgggt tccggaggtc agccgctacc tgatgctgac
    10741 cgcttccggc ccagaagacc cgatcggcca cgccatcacc ctgcgcactg cggtccaccc
    10801 atgggggccc tggtcgccaa ggcgccggct gctcgactgg gtggcgaccg gtatggcgca
    10861 ccacgatccg cacacccggt tcatcaaggc tgcgaccgac ggttccgacc cggtcggcga
    10921 ccaagtgttc gcggcacagg cgcgcgggac gggcgccgcc tacgcgccct acttcttcga
    10981 cgccgccgtc gacggcgaag agctgctgct ccgctacacc ctctcgacgt ggaaccccta
    11041 ccaggtggtg ctgatggccc accgcctccc gatcgccgcc ctgtaggccc gccagctgaa
    11101 cgaaagggga cggttccttt tccgttcaag ttatccacag gccggacggg gcgggaaggc
    11161 gcgtcctcta cgctatgagg gcaatcgaag cggaggtgag cgtgcgcaca gagtcggtgc
    11221 gaatctgggc gtggcgagtc ttgcttgtcc taccgccgct gctcgccgcg attcatgccg
    11281 gcggcaccca gattccggga gccagcctgc tgccgtggca tccgggcatg atcgatctcg
    11341 aggtctatcg ccggaccggc gagctggtgc tcagcggcgg cgacatcttc aatgctgagg
    11401 gactgccctg gatctatccc ggcttcgccg ccctgctcac ggtgccgttc gcggtggtgc
    11461 cgttcgaact ggctgcggtg gcctggctgg cgctgtccgc cgcagcgttg gccgcgatca
    11521 tctatcggct gggctacacc ggctggcagc tctcggccct cacgacggtg tgcattctct
    11581 tcgtccatcc ggttcgggag accctcggct tcggccagct cggcatcttc ctggtggccg
    11641 cggccgtgct cgactcgatg cccgggccgc gggtgttcaa gcgccggatc ctgcccgagg
    11701 gctggctggt cggggtcgcc accgcggtga agctcactcc cgcggtggtg gccgcctaca
    11761 acttcttcgc cggacgtcga aagccgggtc tggtcgcgtt cgcgtccttc cttgccgcga
    11821 cggcgctcgg gttcgtgctg ttgccgcagg cgtcctttgc ctactgggcc aagctcgcgt
    11881 ccggcgattc ggggctcaac tccggaatcc cgtacgccac gaaccagtcc gtcctcggta
    11941 tgtggaaccg gctcaccggc gagcccggac gggtggggct gctgctcagc gtcctggtgg
    12001 tgttcctcgg ggtgtgggcc gctgtgctga tgcatcgccg cgggcaggtg gcgctggcga
    12061 tctgccttgc gggcctgacc agcctgctcg cgtccccgat ctcgtggtcg caccactacg
    12121 tgtgggtggt gccgcttggg ctggtcctct ggcaggcccg cgaactgccg ggctacctgc
    12181 gctggccagg actggcgtat gtgctctggg ttgtggtttc gccgttcatg tggctgccgc
    12241 gcggggacga tgtcgaactc agctacgcgc cctggatgca gttcgtcgac aacatcggca
    12301 tcgtggtcgg ggtcttgctc ctggccggtt cggcggcgac cgccctcggc ccatggggac
    12361 gcaagcaggt cttgccatga gatccacgag ggtctggttc atcgtcgcgg ctgcgttcgc
    12421 cttcgcgtcc gtggtgatgg ggacggtggt ctgcgcgacc gagtcgggcg cggcctgtcc
    12481 caactggccc ggatgctatc ccgaccagtt cgcgccccag gcgcagctca acccgatgat
    12541 cgaattcctc catcgggtga tcgctgcgct gaccggcccg gtggtgctga tcgccgcgat
    12601 cctggcccgc aaggtggccg acccgcggcc gcgctggctg gcctggatcg gtctcgccgg
    12661 gacgatctcg gccggcgtct tcggcatgct gatcgtccgg atcggcattc cctggtggct
    12721 cggggtcgtt gatctggcca gcgcactgat cgcgaccgtc gcgctgatcg tcgcggccct
    12781 gctgatgcgt ccaggtgcgg cttggacgcc cggacgcacc gcccggctcg cctgggcagc
    12841 ggtcggcacg ctcgcggtca tgcacctttc ggcgcttgcc gtggccggcg acaatgcgtt
    12901 cacccgctgc atcagctggc cgttgggagt cttcgaaacc gaccactggc cggtactgca
    12961 gtggctgcgg atcgtcctgg cgctggtcgc ggcggtgctg atcgtcctgc tggtcgcgga
    13021 cggagtgcgc accccgcggt tgcgattgcc ggcgctggcg agcggcgtcc tgctcgtgct
    13081 tgagttgatg ctcgtgccgg tattgctcgg cggctacgac gacctggtgc tcgtgacggc
    13141 gtacgcggtg actgcagcgg cactcttcgg ctcggtcagc ctgctggccg gacgggcttc
    13201 ggtgcggctc gacgtgccgg tgggcgcggc ggacgcgtcc gcgttgacgg tcgcctgacc
    13261 gaattccgcc gtctcgacgg ccccgtcccc ccactcaagc cagcgaaact gaccaaagcc
    13321 agcgaatgtt ccccggcttg tgtccggatc gccggctgga acgtcggacg gcgtcctgcg
    13381 cgaacggcgg cgacggaata tgattgcgcc taagctagtt gagtctggtg aactcaactt
    13441 tgctttgctc aaggagaact agcccacatg gacaccgaaa agctcactgc gcgcagccgg
    13501 gacgcggtct cggccgccgt gcgcaccgcc cttaccaacg gcaacccgaa cgctgaacca
    13561 gtccacctgc tgcacgcgct gctgctggtg ccagagaacg cggtcgcgcc gctgctggcc
    13621 tcgatcggcg tcgatccgac cgtggtggac gcagccgctc gcggcgcgat caccaagctt
    13681 ccgtccgcgc agggttcgtc ggtcagccag ccggcaatgt ccggcggtct ggcccgcgtg
    13741 ctggcggacg ccgagacccg ggcccagcaa cttggcgatc agttcgtggc caccgagcac
    13801 cttttgctgg cgctgtcggc cgtccagacg gacgcgaaga agatcctcaa cgaccttggc
    13861 gcgaccaccg acaagctcgc caaggcgttc aacgacgccc ggggctcgaa gcgggtcacg
    13921 tccgctgagt ccgagggcgg cgagtccgcg ctggacaagt attcggtcga cctcaccgaa
    13981 cgcgcccgcg agggcaagct cgacccggtg atcgggcgcg attccgagat ccgccgggtc
    14041 gtccaggtgc tggcccggcg caccaagaac aatccggtgc tgatcggcga gcccggcgtc
    14101 ggcaagaccg ccgtcgtcga aggcctcgcc cagcgggtgg tggccggcga cgttccggat
    14161 tcgctgaagg gccggcgggt ggtgtcgctc gacctggcgt cgatggtcgc cggggcgaag
    14221 taccgcggcg agttcgagga gcggctgaag gccgtcctga acgagatccg cgatgccgag
    14281 ggccaggtga tcaccttcat cgacgaactg cacaccgtgg tcggcgcggg cgcgactggc
    14341 gattcgtcca tggacgctgg caacatgctc aagccgatgc tggcccgcgg cgaactgcgg
    14401 atgatcggcg cgaccaccct cgacgagtac cgcgaacgca tcgagaagga ccccgcgctg
    14461 gagcgtcgtt tccagcaggt cttcgtcggt gaaccgtcca tcgaggacac gatcgcgatc
    14521 ctgcgcgggc tgcggcagcg ctacgaagcg caccacaagg tgcggatcac cgattcggcg
    14581 ctggtcgccg cggcgacgct atcgggccgc tacatcacca gccgacagct gccggacaag
    14641 gccatcgacc tgatcgatga ggctgcgtcc cggctgcgga tggagatcga ctcctcgccg
    14701 gaagagatcg atcagctgcg ccgcgacatc gatcggatga ccatgcaggc cttcgcgctg
    14761 gagaaggagg acgaccccgg ttccaaggac cgctacgccc ggctgaccgc cgcgctggcg
    14821 gacgccaagg aggaactccg cgggttggag gccaggtggg aggccgagaa gtcggggctg
    14881 aatcgggtcg gcgaggtcaa ggagagcatc gacaagctgc ggatcgaggc cgagcgtgcc
    14941 cagcgcgagg gcgatctgac gaaggcttct gagatcctgt acggacgcat cccggcgctt
    15001 gaggccgaac tggagtcggc caccgaagcc gagcagcaga ccaagcagat ggtcagcgaa
    15061 gaggtgtccg cgaccgacat cgccgaagtg gtttccgcgt ggacgggggt cccggtcggc
    15121 aagatgctgc agggcgagag tgagaagctg ctctcgatgg aggatcgcct gggcgagcgt
    15181 ctgatcggcc agcgcaaggc cgtcaccgcg gtggcggacg cggtgcgccg ctcgcgggcc
    15241 ggcatctccg acccgaaccg gccgaccgga tcgttcctgt tcctcggccc gaccggtgtc
    15301 ggcaagaccg agttggccaa gtcgttggcc gagttcctct tcgacgacga gcacgcgatg
    15361 gttcgcatcg acatgagcga gtacggcgag aagcacaccg tctcgcggct ggtgggcgcc
    15421 cctcccggct acatcggcta cgaggagggc gggcagctca ccgaggcggt gcgacggcgt
    15481 ccgtactccg ttgtactact cgacgaggtg gagaaggcgc atcccgaagt gttcaacatc
    15541 ctgctgcagg tgctcgacga cggccgcctg accgacggcc agggccggac ggtggacttc
    15601 cgcaatgtga tcctgatcat gacctcgaac ctcggttcgc agttcatggc cgacccgaag
    15661 ctgcacgcgg acaccaagca ggaagccgtg atgaacgtgg tgcggcaggc gttcaagccc
    15721 gagttcctga accggctgga cgagatcgtc atcttcgacg cgctcggcat cgaggaactg
    15781 tcgcggatcg tcgacatcca gctgcgtcgg ctcaaccagc ggctatccga tcgccgaatc
    15841 gagattgagg tctccgatgc cggcaaggag tggctggcgc tcaccggttt cgacccggtc
    15901 tacggcgcgc gcccgctcaa gcggctggtg cagaccacga tcgaggacgc gttggcgcgc
    15961 cgggtgctct ccggtgaggt gagcgacggc cagaccgtcc gctttgagct gaacgaggac
    16021 ggctccggcc tggtgctgag ctgattcagc ggacaaaacg ccctgtcgac ggcccagtga
    16081 accccgagag ccgggattcg ggcatcggca gggcattttg tccggacctt catcacagac
    16141 tcttcgctag gctccaaaca tgaggtcccg ccacaccgcc cgcgacgagg tgacctacaa
    16201 acgtcgccac gtgggcaagg tggagtactt caccagccgc tacgggcgcg gggacgcgtc
    16261 catggtggtc taccgattcg cacggctagg tgagcggagc gggccggact tcgtgctcgt
    16321 gcatggcatc ggggtatcgg cgcggtcgta cggaccgacc gcggtggagt tggcgcaaca
    16381 cggggacgtc tacctgatcg atctgccggg ctatgggcgc tcgccgcgtc cgggcgtcga
    16441 cctcagcatc gccgggcatg cgcaggtgct gggcgacttc ctgaccgatc gcgaacttga
    16501 ccatccggtg gtcgtgggcc attcgatggg ctcgcaggtg gtggtggaac tggccgccgc
    16561 ccacccggaa caggtcgacc acatcgcgct gatcgcgccg gtgatcgtgc cgtccgcgcg
    16621 caagctgccg aagctgctga ggctcttcct ccaggatgtg gtgaaggagc cgccgcaggt
    16681 ggggctgatg gcggtttacg actacctgtt ccgggccggg gtgccgtaca tgatcgagca
    16741 gactccgcac ctgttcggcg cgcgggtgga ggagactgcg gctcgggtcg aggcgaagac
    16801 gctggtcatc tgcggtgagg acgatcctct ggtccccctg gactggggcc gggagcttgc
    16861 cgacgacttc cggcacggct ggttcgcgac ggtcccgggc ccgcacgcga ccatgttcgc
    16921 cgcgccggag cggatcgcga agctgttgga tgagcatgcc catcggtagc ccgggagccg
    16981 atgcggcggc gcggttcaag ctcgcggtgc gtcgtgcgcg ctactgggcc cgcgactatt
    17041 tctatgtgac gcatcgccag ctgcgcgggc ccctggagcg ggcggtgccg accgcgttcg
    17101 cgtccggtga tcgggcgccg gtggtgctgc tggccggcgt cctggagccg tggacgctgt
    17161 tgctaccggc agccgagcgg ctgaaccggg acggacaccc ggtgcaggtg gtgcccgaac
    17221 tcgcgtacaa cctggccacg ctggacgcgg ccgccgaact cgcgatgaac gcggtgaccg
    17281 ccaaggaact gaccggcgtg atcctggtgg cgcacagcaa gggcgggctg gttggcaaac
    17341 gcatgctcga actcgacgcg gacggacggc tgcgcggcct gatcgcgatc gcgacgccgt
    17401 tcggtggatc gagcctggcc aggttggtgc cgtcgcggac gatccgcgcg cttggcccgg
    17461 aggatccggc gatccaggcg ctggcgaagc gcagcgacct gaatccgctg atcacctcca
    17521 tctatccgtc gttcgatccg cacattccgg acggctcgca cttggacggc gcggtcaaca
    17581 tcgaggtggc cgcgatcggg cacttccgcg tgctcaagga cgccgaagtc ttggacgcgg
    17641 tcgcggccgc agccgagcgc ttgtcgcagg cctgattact ccggaagctc aagctccgga
    17701 tcgatcagca cggcgggctt cacgcccaac acgtcggcca gcaggtagat ggtgtcgagt
    17761 cgcgggttgc ccctacccag gcggcccgtc ttcggatcgc gggtgttatt gcgcgaccgc
    17821 tcgatattct ggatctggtt gcggctcatc ccaaccttgt gggcgagctc ctcctgcgtc
    17881 cagtggcgcc gttcgcgaag ctggcgcact ttctgaccca gcgacccagc gacctcgtcg
    17941 agcatccggt cggtgagggg catggccccc accctgtcgg cggcccttgt tcgaagtcag
    18001 cccaacagat tgggtattgc tgaacaacgt cattacgcct tgccgggcgg ggctctgctc
    18061 gtcgccatgt accgactaca caacctgcct ctgacagttc tgggcacgcc cagcttctcc
    18121 ggcgtcgact ggcgcgatat cgtcgccgag tcgtcgcgcc gctggtgagt tcctgctcct
    18181 tcttggcatc ctcgatgccg ttacggtagg ccagtggaag aggaagtgga cgcgctgctg
    18241 cgtcacagcc ggtcgattcg cagctttgtg accgcggacg ggctgcgccg caggcgcgaa
    18301 gacaacccgt cgtccgccta ccaggaggcg gccgagaatt tgctgctgaa ggcgtggcgt
    18361 gaactcgtcg atgacccggg acgcgcggtc tcatatgtcg agcgagcgtt gcggctgccg
    18421 ttcgacccga acgaggactc ggcgcccgcg gcgtacgccg cgcacatgct gctcttcaac
    18481 ctggtcgtcg acgcactgga ggattccgag gagggggagg aggagtggct cgcggccgcc
    18541 atccaagtgc tcaacagcgc tgacccacgc gccagctccg aacttcggga cgtcctcgaa
    18601 gtcgtcgcgc aggactacca catcacgtcg tccgagcgcc gcaagttggc ggcagcagtg
    18661 aaatcagccc cacctcgcca atcgctgttc gacctccagc tgcctccaga cgagctttgc
    18721 gatcacatcg tcgcggtcct ccacgcctgc gaagcgtacg agaccgcgct tgacgagctc
    18781 ttcgcctgag cctcaccggc ggagcctcaa gtatccgaat gcttgcaact cagcccggat
    18841 tcgccgctgc ggctcaggcg gcgacgtgcg cctgcagcag ccagcggtcc ttggtgagcc
    18901 cgcgctcgat ctcgatggct acatcctggg tggacctgct caaccagccc ttaccaatat
    18961 cgattccatt tcatctgggg tttatgctcc gcgacatggt cagcaaccgg gttgtgccgg
    19021 ctccggacga ccggcgtgcc ttcatggtgg tggccggcaa caccgagcag tcctgggtgg
    19081 acccggccaa cccgctgcgg ctcgagttcg aatacgtgca gcgcatcgct gaaattctcg
    19141 acctcaccgt gctgtcccgg cccgcggccg aacgcgtccg catcgtccac gtcggcggcg
    19201 gcggactgac cctgccgcgc tacgtcgcgg cccggcgtcc gcacaccgcc cagatcgtcc
    19261 tcgaaccgga cgaagaactc actgcggagg tccgcgcccg gctgcccctg ccgcgcaact
    19321 ccggcatcaa gatccgggcg cacgacggcc gcagcggcgt cgcccaactt ccggacgcct
    19381 atgccgacgc cctgatcctc gacgccttcg ctgctgccag tgtccccgcc gaactagcca
    19441 ccgtagaatt cttcacggac gtgcgccgcg ccctgcgtcc cggcgggttg ctcatcatga
    19501 acctcaccga tcaggcgccc ttcggctggt ccaaacgctg cctcgccgcg ctatttgcga
    19561 cccagccaca gctggtcgcc agcgccgaag tgccggtctg gaagggtcgt cgcttcggca
    19621 acctggtcgc ggtcgccggg cccgatcttc ccgaaaccga cttcagccgc gcccttgccc
    19681 gtgcgccgtt tccgtatcgg ctgctggccg gacgcgaact cgtccgcttc atcggcgcgg
    19741 ccacaccctt caccgagctg gacgcggagc cgtccccacc tccggcctgg agtcgcacct
    19801 ggttctcgtg agtcacccga ttcgagttga gatcgagccc tacgatccgg ctctgacccc
    19861 tacggttgcg tccatgactg cgcgcgacga actcatcgaa ctgatcaaga gccttgccgt
    19921 ggtgcgcggc aaagtcaccc tcgcctccgg caaggaggcg gactactacg tcgatctgcg
    19981 ccgggtaacc ctggacggcg tcgccgcgcc gctggtcggt gaggtcatga ccgaactcgt
    20041 cgccgactgg gatttcgttg ccgccggggg actcactctc ggtgccgacc cggtcgcagt
    20101 cgccatgctg caccacgctg cccacaccgg cggacgcctg gacgccttcg tcgtccgtaa
    20161 ggagtcgaaa gcccacggac tgcagcgccg catcgagggc gccgaggtcg ccggacgccg
    20221 cgtcctggtc gtcgaagaca cctccaccac gggtggctcc gtcctcactg cggttgacgc
    20281 ggtgcgcgaa gccggcggcg a
//
LOCUS       DAOPRQ010000047        20279 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_2241_length_20279_cov_11.821252, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000047 DAOPRQ010000000
VERSION     DAOPRQ010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 20279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20279
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2241_length_20279_cov_11.821252"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="PKJ61_07495"
     CDS             complement(<1..305)
                     /locus_tag="PKJ61_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004811403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HOC13707.1"
                     /translation="MARYFDVHPSNPQPRALAQVVEILRSGGLVAYPTDSGYALGCRL
                     GNRDGLERIRTLRNLNDKHHFTLVCSEFAQLGQYVQMNNDVFRAVKAATPGAYTFILR
                     "
     gene            346..1482
                     /locus_tag="PKJ61_07500"
     CDS             346..1482
                     /locus_tag="PKJ61_07500"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOC13708.1"
                     /translation="MSLLSVPLAELRERRSLKWQVYASDVLPMWVAEMDTDLHPAVRA
                     TLKAAIDRGDTGYPYGTAYAEAFAAMADARWGWQLDVAAQARRAGDVMNSILAVLEAT
                     TSPGDAVVINPPVYPPFRQVVGGYGRRIVEAPLTPSGRLDAESLAAAFSGPDRPKAYL
                     LCSPHNPTGAVHTSAELETALALASEHGVQVIVDEIHACLVDPGTTFVPALAAPGGER
                     AISVTSAGKAWNLAGFKAGLIIAGSQATKVLAALPPLAQQSSGHLANLVHTVALTEAQ
                     DWVDHLMVEIAANKALLATELARELPGAQYVRTSGTYLAWVDCSGLGLANPAAHFEQV
                     GRVAFSPGVNFGQEHQQWVRINLACSPELVVEGVRRMASALATG"
     gene            complement(1537..1929)
                     /locus_tag="PKJ61_07505"
     CDS             complement(1537..1929)
                     /locus_tag="PKJ61_07505"
                     /inference="COORDINATES: protein motif:HMM:NF024792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HOC13709.1"
                     /translation="MSNDQRGVSLSAMFAVIVLALLLMAGLVVDGGAQAEAARRAESA
                     AAAAARAAADETATARLAGKPVDTGAAIVAAQRVLDAAQVSGTVSLAGGLVRVSTRAE
                     AGTVFLSLIGITTLDAAGSAEAELVAND"
     gene            2067..3293
                     /locus_tag="PKJ61_07510"
     CDS             2067..3293
                     /locus_tag="PKJ61_07510"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013161100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrophosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HOC13710.1"
                     /translation="MVQKVALLTAGGFAPCLSSAIGGLIERYTEVAPDVEIIAYKNGY
                     EGLLSGDYLTVTEAIRAKAALLHEYGGSPIGNSRVKLTNAKDLVKRGLVAEGDDPLKV
                     AADRLVADGVDVLHTIGGDDTNTTAADLAAYLATNGYGLTVVGLPKTIDNDVIPIKQS
                     LGADTAAEMGARFAKNVMAEHNAGTRILLIHEVMGRNCGWLTAATARKYHEWIESVDW
                     LPEIGLSKKSWDVHAVFVPEAEIDLHAESERLLKVMDEVGCVNIFLSEGAGITDIVEE
                     MEKAGQEVARDAFGHIRLERINPGAWFGKQFAAMLNAKKVLVQKSGYYSRSAPANEFD
                     RALIRSMTDLAVDSAIEGKPGVVGHDEENGDVLSVIAFDRIKGGKPFDITEDWYLELL
                     SEIGQPGPVAAAPVEH"
     gene            complement(3387..4115)
                     /locus_tag="PKJ61_07515"
     CDS             complement(3387..4115)
                     /locus_tag="PKJ61_07515"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HOC13711.1"
                     /translation="MVPSADAAARERLLAEAGHARYLAGRLAVELPRPLGIGTPFERY
                     PGLWSVWSWVDGVSVDRSSGLDQNLLAADLAALLRCFHELPTDGQSWSAAGRGARPLA
                     DSAWVRSSIERSAHLVDAVAVTSLWERALAAPGFTQPASRIHGDPMPGNLIVRHGRLA
                     GLIDIAEPSFGDPASDLAPAWTIFDEPARTLFGQVLGLDEAAWERGRGWALEVAIGGL
                     HHYERTNRGFYDQAARTISRLLEE"
     gene            complement(4122..4277)
                     /locus_tag="PKJ61_07520"
     CDS             complement(4122..4277)
                     /locus_tag="PKJ61_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13712.1"
                     /translation="MMHARQVEVALDCLAAALGEQFPQLRGLAVRRISSAGTVVAPVR
                     VGDSFVA"
     gene            complement(4277..5425)
                     /locus_tag="PKJ61_07525"
     CDS             complement(4277..5425)
                     /locus_tag="PKJ61_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOC13713.1"
                     /translation="MTRLSRRGQVEPFHVMETAAAAARRQDTHGDAILLCVGQPATPA
                     PLVVREAARAAIKQKVLGYTVTAGIPELRATIAADYADRYHVGVDPADVVITTGSSAG
                     FQLVLLAAFDAGDTVAMTRPGYPAYRNTLLSLGARVIDLEAGPQTRYQPTVAMLEALP
                     EPPAGLVIASPANPTGTIIDPGELAELAAWCAANDCLLISDEIYHGISYGRDCVTVRQ
                     FSADAVAVGSVSKYHSMTGWRLGWLVLPRALRRPVELLQGNLAICAPAISQVAALAAF
                     AAEARAELDGHVARYARNRDLLLRRLPELGITAFAPPDGAFYAYCDVSHLTTDSQRWC
                     ADVLAATGVALAPGIDFDPVAGNRMMRLSFCGQTDELAEALDRLARLT"
     gene            complement(5422..6507)
                     /locus_tag="PKJ61_07530"
     CDS             complement(5422..6507)
                     /locus_tag="PKJ61_07530"
                     /inference="COORDINATES: protein motif:HMM:NF015587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HOC13714.1"
                     /translation="MEAALAWWAEFSKRPFVAHILRTVERFNVRGGGQLAAAIAFFSV
                     LSLVPILMLAFSALGFTLTVFMPEALGVIQTWINGQLDTDSDFGRNIHEVIERALTSW
                     ASTGLVAMGIMMWVGSNWIGNLKRAIRLLMRAEVDNPGRTLPLPLDVLANFAGLVGVL
                     VGVAATFVASAIASGFGGALGDLLGISNSLLWTVVLRLVSALVSLAAGAVLFRLLFGW
                     FSPHPVPSHLAWVGAGIGSAGLLILQSFTGILIGAFSRNLGTAVFTWTLVLMLFLNLF
                     ATLILMIAAWLATAEEPEPEVLPELVVTEPTLRSLPTKPGQEVVSAEVARKSMGVGLG
                     AGYIVGSATGLGLGALLVGALAKLRRK"
     gene            complement(6514..9372)
                     /gene="gcvP"
                     /locus_tag="PKJ61_07535"
     CDS             complement(6514..9372)
                     /gene="gcvP"
                     /locus_tag="PKJ61_07535"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016669947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HOC13715.1"
                     /translation="MNDFTQRHLGAVASDRFEMLAALGYASLDELTDAAVPVAISLRE
                     RLDLPEPRSEFEVQRELAAMAAQNRPGLSMIGLGYHGTVTPPVIRRMILENPAWYTAY
                     TPYQPEISQGRLEALANFQTVIADLTGLPITGASLLDEGTAVAEAVAVAHRVTRKGGC
                     VVVDPQLLPQSLAVLRTRCLATGIDIVVADDSVADSVRAHQCFAVVVQVPGADGVLPS
                     ATELGALAQLAHDNGAQLIAAVDLLALTLIGSAASWGVDIAVGSAQRFGVPLFYGGPH
                     AGFIAVREGLERQLPGRIVGISKDADGIPAFRLALQTREQHIRREKATSNICTAQVLL
                     AVTASAYAVHHGPEGLTAIARSVHERTRRLAAALAAGGLELASDAFFDTLTVTVPGRA
                     DDVVESARDQGIHLRRSDSDQVGISLGEDTDEAALVKVCEAFGVAPPNASRFGGLGDH
                     ERTEPFLTHPVFSAYRSETEFMRYLRTLADRDFALDKGMIPLGSCTLKLNPAAAMEPI
                     SYPGFAGLHPFVPEAAAQGYARMIGELCDWLAEITGYDRVSLQPNAGSQGEFAGLMAI
                     RSYHLANGEPHRVVCLIPASAHGTNAASAVMAGMRVVVVATAADGSVDLTDLAVKIDK
                     HAAELAAVMVTYPSTHGVFEETITEVCDRVHAAGGQVYVDGANLNAMLGLARPGKFGA
                     DVSHLNLHKTFAIPHGGGGPGVGPVAVRAHLAPYLPNHPLEPSAGPANSYGPISAAPF
                     GSAGVLPITWAFIALMGADGLREASQSAILAANYVARRLREHFPVLYTGAYGLVAHEC
                     IIDLRELTKQTGITVDDVAKRLVDYGFHAPTMSFPVAGTLMIEPTESEAKAEIDRFCD
                     AMIAIRAEMDAVAAGVWPADDNPLHNAPHPVSRILADEWTHPYSRELAAYPAGVRRGP
                     IGSGGRDKYWPPVARIDQAYGDRNLVCTCAPVADYASE"
     gene            complement(9460..10014)
                     /locus_tag="PKJ61_07540"
     CDS             complement(9460..10014)
                     /locus_tag="PKJ61_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOC13716.1"
                     /translation="MNAMNQVPPAQDLLFDGDFSPLPDDLGFRGPVACRAADITYRQL
                     DYWARTGLVVPEVRPAGGSGTQRLYSFRDLLILKVIKRLIDAGISLQQIRTAIDHLRA
                     RGVQDLTEVTLMSDGVSVYECTTDDQVVDLLRGGQGMFGIALGGVWRDIEGTLQQLPA
                     ERAEDHPQPADDELSRRRQARAVG"
     gene            complement(10136..10645)
                     /locus_tag="PKJ61_07545"
     CDS             complement(10136..10645)
                     /locus_tag="PKJ61_07545"
                     /inference="COORDINATES: protein motif:HMM:NF014618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HOC13717.1"
                     /translation="MRELKVIEIRVSTGEAPPLVVLAEVGGAERRIPIWMSHGGASAI
                     FSATEPPDDQRPSIHDVAWRLATSSSEPLSAVTITRYEAGQYYAELVFGETVLPARPS
                     DAIAIALRSGRPIQCSEELLETVGVSAEELGTAPADGVETPEDEVERFLEFLDSVTPE
                     DFTGDEGKP"
     gene            complement(10654..11349)
                     /locus_tag="PKJ61_07550"
     CDS             complement(10654..11349)
                     /locus_tag="PKJ61_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOC13718.1"
                     /translation="MAIGLRSIGQVLAVLKPEFEDVSISKIRFLESEGLISPERAPSG
                     YRRYAESDIERLRYILDVQKNHYLPLKVIREHLDMMDRGQEPPVLGTNRPAEGATPVE
                     SPAAISGQAHAPKRAIRITRRELLELSGLAEAALSDLEKHQLVVPRRGTIYYGRDALT
                     VAVIARKLAAFGMDARHLRVVKQAAEREVGLIEQAVAPHLRRNPSARTLPADVMQLVL
                     HAHAAIMRSQLPQ"
     gene            complement(11349..11831)
                     /locus_tag="PKJ61_07555"
     CDS             complement(11349..11831)
                     /locus_tag="PKJ61_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016456539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOC13719.1"
                     /translation="MSTCPACGFELVEPVNFCPHCGASLAKLSGDTTRVIPAVVDEFQ
                     TEDLTPADSAAVEALPPGSAILLVLRGTHAGARYLIDSDVVTSGRHPRCDIFLDDITV
                     SRHHARFVRRGSEFWVSDQNSLNGTYVNKVLVESDVLLKTGDEVQIGKFRMVFFTGHD
                     "
     gene            complement(11901..12281)
                     /gene="gcvH"
                     /locus_tag="PKJ61_07560"
     CDS             complement(11901..12281)
                     /gene="gcvH"
                     /locus_tag="PKJ61_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HOC13720.1"
                     /translation="MYPDDLQYSSKHEWVRQGAGGTVRIGITSYAAEALGDVVYVSLP
                     QVGEEIAVDDACAEVESTKSVSDVYSPATGVVTAVNELLNDTPETINSDPYGDGWIFE
                     LELSDPEELDELLDADGYAELVSG"
     gene            complement(12355..12963)
                     /locus_tag="PKJ61_07565"
     CDS             complement(12355..12963)
                     /locus_tag="PKJ61_07565"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002547832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOC13721.1"
                     /translation="MAGEPYDTERVLTVPNILSFIRLLGVPLFLWLLLIREADVWAVV
                     VLAIGGATDWLDGHLARLWHQRSRLGQVLDPVADRLYILATLLGLAIREIVPWWLVVL
                     LAARDVLMVVTIGPALRRRGFASLPVHFLGKAATFMLLYGLPLVLLGAGDWLMPTTAR
                     VLGWACVIWGAGLYWWSGLLYLSQGAVVIRRIPVGTGDNSAS"
     gene            complement(13492..14052)
                     /locus_tag="PKJ61_07570"
     CDS             complement(13492..14052)
                     /locus_tag="PKJ61_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020384742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HOC13722.1"
                     /translation="MTEPGEAREVVEDAGTLAGVPDAFERLWTPHRMVYIEGENKPVD
                     GASHSCPFCRAPEREDADGLVVRRGRTVYAVLNLYPYSPGHLLICPYRHVSDYTDLTA
                     EELLELAEFTAAAMTVIRRVSKPAGFNLGLNQGQVAGAGIAAHLHQHVVPRWPGDANF
                     LPVIAQTKALPQLLADTRDQLASAWA"
     gene            complement(14049..16034)
                     /gene="thrS"
                     /locus_tag="PKJ61_07575"
     CDS             complement(14049..16034)
                     /gene="thrS"
                     /locus_tag="PKJ61_07575"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017933636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOC13723.1"
                     /translation="MSVSIIVIDPQAQPSEQAVTTGTTALDLFGTDRSIVAARVNGAV
                     VDLATELREGDQVEPVLATSDEGLAIIRHSCAHVAAQAVQLLHADAKLGIGPPITDGF
                     YYDFRVETPFTPDDLKAIEKAMVGIVKERQQFVRRPISDEAAAAELASEPYKLELIGL
                     KGAAANTEGASVEVGAGELTIYDNVRRDGAVAWKDLCRGPHVPHTGYLGNGWALTRSS
                     AAYWRGDQRNDQLQRVYGTAWASKDDLVAYRTRLEEAARRDHRKLGAELDLFSFPEEL
                     GAGLPVFHPKGGVIKRVMEDYVRTAHIRSGFQYVGTPHIAKEVLFRTSGHLPFFADGM
                     FPPMDDEGQNYYLKAMNCPMHNLIFASRGRSYRELPLRFFEFGTVYRNEKSGVLQGLT
                     RVRSITQDDSHSYVMASQAAEEVKHLLTFVTNLLEDFGLDDYYLELSTRDTEGAKSTK
                     FIGSDEQWEKATAVLEKVGIESGLKLVPDPGGAAYYGPKISVQAKDAIGRTWQMSTIQ
                     YDFNQPARFGLTYTGPDGHPAEPVMIHSAKFGSIERFIGVLTEHYAGAFPAWLAPVQV
                     VAIPVAEEYEGYLSEVAKQLIAADIRVEVDRSDDRFGKKIRNAQTQKVPFMLIAGETD
                     VAAGAVSFRYRDGSQRNGVPIAEAVAEIIAATRPPRR"
     gene            complement(16104..16361)
                     /locus_tag="PKJ61_07580"
     CDS             complement(16104..16361)
                     /locus_tag="PKJ61_07580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13724.1"
                     /translation="MIEGLALVGKTLTVHPGVWGPAGVRTTVRWYADGVLLTRAMGDT
                     VVLSGAEAGKLIRVRVSGELEGYADRIIDSLAVGPVVSRER"
     gene            complement(16354..16953)
                     /locus_tag="PKJ61_07585"
     CDS             complement(16354..16953)
                     /locus_tag="PKJ61_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13725.1"
                     /translation="MQSPPGSTPTAPPALVPSAVPEIGGTPRYRSVLSIEEPDWGPGR
                     VEQEYRWLRDGKPISGASELSYRLAAADIGHKVSLRISGYKAGFPKLAQETATVGPIE
                     PGRLVMATPLIRGSARVGRTLTGSVDAGGPGEVDQRWQCYRGADKIPGATSGSYQLTL
                     ADLGKVIKPRTRLPRQGLQVLRLHQCHRAGRPHPGSAGD"
     gene            17081..17926
                     /locus_tag="PKJ61_07590"
     CDS             17081..17926
                     /locus_tag="PKJ61_07590"
                     /inference="COORDINATES: protein motif:HMM:NF013397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HOC13726.1"
                     /translation="MTTDHMRLFPGKFFISTALEALETKTAMSKELAVRYLQRAAIAG
                     LLIAIFYLAHFGAVAAFGSVQVGTGSLAVLGKMVGAVIFGWALVFIYFTKSELLTSNM
                     MVASIGIYYRRVKWGRALRMLGLCYVGNFVGGLVLAALARLSTLASGAVLSEMEHSVT
                     VKLGYISAGPLGWSDLLMRAILCNLMINVAMLLVYNGFIKDDLTKSLAMIMAVFIFVF
                     LGLEHSVANTILFTIVGLKSGIDLAPAAGNVAIALIGNFIGGGVLIGLYYAYVNDDSK
                     YRANP"
     gene            17945..19018
                     /locus_tag="PKJ61_07595"
     CDS             17945..19018
                     /locus_tag="PKJ61_07595"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOC13727.1"
                     /translation="MADKPKWWFRKPALNWLELLGISLALALIGGALGVTAALKLSPE
                     PGACDSVRVADSVLPSVVTIFVTGASGGGSGSGAIIRADGLILTNDHVIAAGVESGNI
                     EVLLNGGEQVPADLIGTDPITDLALLKIDKNKLPTLLLAPREPLSVGQPVVALGAPLG
                     LSGTVTSGIVSALNRNVPVPKASGGTTVLTGAIQTDAAINPGNSGGPLVTCEGRLVGV
                     NTAISTVPNASGVAGGGSVGIGFAVPASTAQRIVDELLATGRATHPWIGAVTAEITQD
                     MADRFGTPSGLFVQDVSEGGPADAAGLEAGDIITRLSGNPATSLGLGWLLVSAKVGDD
                     IPIEFARDGVLQNATLTLAEQPA"
     gene            complement(19095..>20279)
                     /locus_tag="PKJ61_07600"
     CDS             complement(19095..>20279)
                     /locus_tag="PKJ61_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015071605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HOC13728.1"
                     /translation="ADARPPTRDDVDLIREILIGRDLPGRDVVAIAEDRASIADRLTM
                     EQAQLLSVTRLLNRVEIRGGAGSGKTVLAIRQASDLAGGRATGERQRVAVVCYSYGLA
                     HHLQRSLLTGSSAKRPAFVGTFEDLARRWGIEITGSRDDSDFWEVELPALMAARAAEL
                     PPESRFDAVIVDEAQDFAEDWWTPLVGALKDETAGGVFAYSDERQRVFARFGRPSLAL
                     VPLVLDHNLRNTRQIAQSFVPLAPTSMVLRGGEGPQVEFVAVPTHAAIEAADDQIERL
                     LEEGWEPKDVALLTMGARHPVQKERQETLGFAGYWDEFWSNDDVFYGHVLGFKGMERH
                     AVVLCVNEDGTRDRSRERLYVGLSRATDRLVVVGDPAVIEAIGGPEVLHQLRAQLPPA
                     RG"
BASE COUNT         3311 a         6880 c         6820 g         3268 t
ORIGIN      
        1 cgcaggatga aggtgtaggc gcccggtgtg gccgccttca ctgcgcggaa cacgtcgttg
       61 ttcatctgca cgtactggcc cagctgggca aactcgctgc acaccagcgt gaaatggtgc
      121 ttgtcgttca ggttccggag cgtgcgaata cgctccaacc cgtcccgatt acccagccgg
      181 caaccgagcg cgtagcccga gtcggtcggg taggccacca ggccaccgga gcgcaggatc
      241 tccaccacct gggcaagtgc tcgcggctgt ggattgctcg ggtgaacgtc gaagtagcgg
      301 gccatggcca acaggctagt gggccgcatc ggtagggtgc tgcccatgtc gcttctgtcc
      361 gtgccccttg ccgaactccg cgaacgccgc agcctgaagt ggcaggtgta cgcctccgac
      421 gtgctgccga tgtgggtggc agagatggac accgacctgc atcccgcggt tcgggcgacg
      481 ctgaaggccg cgatcgatcg cggcgacacc ggctatccgt acggcaccgc ctacgccgaa
      541 gcgttcgccg ccatggcgga cgcgcgttgg gggtggcagc tggacgtcgc cgcccaagca
      601 cggcgagccg gcgacgtgat gaactcgatc ctcgccgtgc tggaggcgac gacttcgccc
      661 ggggacgcgg tggtgatcaa cccgccggtc tatcccccgt tccggcaggt ggtcggcggg
      721 tacggccggc gcatcgtaga ggcgccgctc accccgtccg gacgcctgga cgcggagtcg
      781 ctggcggccg cgttcagcgg accggaccgg cccaaggcct atctgttgtg ctccccgcac
      841 aacccgaccg gagcggtgca cacctctgcc gaactggaga cggcccttgc cctggcgagc
      901 gagcacggtg tccaggtgat cgttgacgag attcacgcct gcctggttga tcccggcacc
      961 accttcgtac ccgcactggc tgcgcccggt ggtgagcgcg cgatctcggt cacgtccgcc
     1021 ggcaaggcct ggaacctggc cggtttcaag gccggtctga tcatcgccgg tagccaagca
     1081 accaaggtgc tggccgcgct gccgcccctg gcccagcagt ccagtggcca tctggccaac
     1141 ctggtgcaca ccgtcgcgct caccgaagcc caggactggg tggaccacct gatggtcgag
     1201 atcgcagcca acaaggcgct gctcgccacc gaactggccc gcgaactacc aggcgcgcag
     1261 tacgtgcgga cttccggcac ctacctggcc tgggtggatt gctccgggct tggcctggcc
     1321 aacccggcag cccacttcga gcaggtcgga cgggtggcgt tctcccccgg cgtgaacttc
     1381 ggtcaggaac accagcagtg ggtgcggatc aacctcgcct gctcccccga actggtggtc
     1441 gagggcgtgc ggcgaatggc gtccgcgcta gccacgggct gagccggagc ttttcctggt
     1501 gccgcggagc cgctgaccga gtggattccc cgacttctag tcgttggcga ccaactcggc
     1561 ctcggctgag ccggccgcat ccaaggtggt gatgccgatc aggctcagga agaccgtgcc
     1621 cgcctcggcc cgcgtcgaaa cccgcacaag tccgccggcc aggctcaccg ttccgctgac
     1681 ttgggccgca tcaagcaccc gctgggctgc gacgatcgca gccccggtgt cgacgggctt
     1741 gccggcgagc cgagcggtcg cggtttcgtc cgccgctgct cgtgcggctg ccgcagccgc
     1801 cgattcggca cgtcgagctg cttctgcctg cgcaccgccg tccacgacca agccggccat
     1861 caacagcagg gcaaggacga tcaccgcgaa catcgccgac agcgacacac ccctctgatc
     1921 gttcgacatt tccccctcca tttccccaac cggcattctg cccggactgc gcgcgtccca
     1981 caccagtcat gcacaaccga tctacgcctc aacgctcggt aggattcggg ctgtatccga
     2041 gttctgcaaa cgattggaag ccaaggatgg tccagaaagt cgcactgctc accgccggtg
     2101 gattcgcacc gtgcctgtcg tccgccatcg gcggcctgat cgagcgctac accgaggtcg
     2161 cccccgacgt ggagatcatc gcctacaaga acggctacga gggcctgctc agcggcgact
     2221 acctcaccgt taccgaagcg atccgggcca aggccgcgct gctgcacgaa tacggcggct
     2281 caccgatcgg caactcccgg gtgaagctca ccaacgccaa ggacctggtc aagcgcggcc
     2341 tcgtcgccga gggcgacgac ccgctcaagg tggcggccga ccggctggtc gccgacggcg
     2401 tggacgtgct gcacaccatc ggcggtgacg acaccaacac caccgctgcc gacctggccg
     2461 cctacctcgc caccaacggc tacgggctca cggttgtcgg cctgcccaag accattgaca
     2521 acgacgtgat cccgatcaag cagtcgctgg gcgccgacac cgccgccgag atgggcgcgc
     2581 ggttcgcgaa gaacgtgatg gccgagcaca acgccggcac ccggattctg ctcatccacg
     2641 aagtgatggg acgcaactgc ggctggctca ccgcagccac cgcgcgcaag taccacgagt
     2701 ggatcgagag cgttgactgg ctgcccgaga tcggcctgtc gaagaagtcc tgggacgtgc
     2761 acgcggtctt cgtgccggag gccgagatcg acctccatgc cgagtcggag cgcctgctca
     2821 aggttatgga cgaggtcggc tgcgtgaaca tcttcctttc cgagggtgcc ggaatcaccg
     2881 acatcgtcga ggagatggag aaggccggcc aggaggtcgc ccgcgacgct ttcggccaca
     2941 tccgactcga gcgcatcaac ccgggcgcgt ggttcggcaa gcagttcgcc gccatgctga
     3001 acgccaagaa ggtgctggtg cagaagtcgg gctactactc ccgttcagcc cccgccaacg
     3061 agttcgaccg tgcgttgatc cgctcgatga ccgatctcgc cgtcgattcc gcgatcgagg
     3121 ggaagccggg tgtcgttggc cacgacgagg agaacggcga cgtgctcagc gtgatcgctt
     3181 tcgatcggat caagggcggc aagccgttcg acatcaccga ggactggtac ctcgaactgc
     3241 tctccgaaat cggccagccc ggcccggttg ccgccgctcc ggtcgagcac tgagtcagtc
     3301 cggccccgtt cggtagctgc gtagcgccgg atcggcgcac ccatctgggt ttaccggtcc
     3361 ggcgcttcgg ctgcgttcgg gtactttcac tcctccagca gccgggagat cgtccgggcc
     3421 gcctggtcgt agaagccgcg attggtgcgc tcgtagtggt gcaggccgcc gatcgccacc
     3481 tccagcgccc agccgcggcc gcgctcccaa gcggcttcgt cgagacccag tacctggccg
     3541 aacagggtcc gggcgggctc gtcgaagatc gtccaagctg gtgccaggtc ggacgcggga
     3601 tcgccgaagc tgggttcggc gatgtcgatc agtccggcca gccgaccgtg gcggacgatg
     3661 agatttcccg gcatcgggtc gccatgaatc cggctggccg gctgtgtgaa gcccggagcg
     3721 gccaatgccc gctcccagag cgaggtcacc gcgacggcgt ccaccaggtg cgcggagcgc
     3781 tcgatcgagg agcgcaccca cgccgagtcg gcgagtggcc gcgcgccgcg tccggcagcg
     3841 ctccaggact ggccatcggt gggcagttcg tggaagcacc gcagcagcgc cgccaggtcg
     3901 gcggccagca ggttctggtc gaggccggat gaccggtcta ccgacacgcc gtccacccag
     3961 ctccagaccg accacaatcc gggatagcgc tcgaacggcg taccgatgcc gagcggacgc
     4021 ggcagctcga cggcaagccg acccgccagg taccgtgcgt ggccggcttc ggcgagcagg
     4081 cgttcgcggg cggccgcatc ggcgctggga accagcggca gtcacgccac gaagctgtca
     4141 cccacgcgga cgggagcgac taccgtcccc gcggagctga tccgccggac ggctagccct
     4201 cgcaactgcg ggaattgctc gccgagcgcg gcggcgaggc aatcgagggc gacctcgacc
     4261 tgcctcgcgt gcatcatcac gtcaggcggg cgagccggtc gagtgcttcg gccagttcgt
     4321 cggtttgccc gcagaagctg agccgcatca tccggttgcc agctaccggg tcgaagtcga
     4381 tgcccggcgc cagcgccaca ccggtcgcag cgagcacgtc ggcgcaccac cgctggctgt
     4441 cggtggtgag atggctgacg tcgcagtagg cgtagaaggc cccgtccggt ggggcgaagg
     4501 cggtgatgcc cagttcgggc aggcgccgca gcagcaggtc gcggttgcgg gcgtagcggg
     4561 ccacgtgccc gtccagttct gccctggctt cggcggcgaa agcggccagc gcggctactt
     4621 gcgagatcgc gggcgcgcag atggccagat tgccctgcag gagttccacc ggacgccgta
     4681 acgcccgcgg cagcaccagc cagcccagcc gccagccggt catcgagtgg tacttgctga
     4741 ccgatccgac cgcgaccgcg tccgcgctga actggcggac agtgacgcaa tcgcggccgt
     4801 agctgatgcc gtggtagatc tcgtcgctga tcagcaggca gtcgttcgcc gcgcaccacg
     4861 cggccagttc ggccagctcg ccagggtcga tgatcgtgcc ggtcgggttg gccggcgacg
     4921 cgatcaccag cccggccggt ggctccggca aggcttcgag catggcgacg gtcggctggt
     4981 agcgcgtctg cgggccggct tccagatcga tcacccgcgc cccgagactc aacagggtgt
     5041 tccggtaggc cggataaccc ggacgggtca tcgccaccgt gtcaccggca tcgaaggcag
     5101 ccagcagcac gagttggaag ccggccgaag acccggtggt gatcacgacg tccgccggat
     5161 cgacaccgac gtggtagcga tccgcgtagt cggcggcgat cgtggcgcgc agctcgggga
     5221 tccctgcggt gactgtgtag ccgagcacct tctgcttgat cgcggcccga gcggcctccc
     5281 gaaccaccag gggtgccggc gtggccggtt ggccgacgca gagcaggatg gcgtcgccgt
     5341 gggtgtcctg acgcctggct gcggcggccg cggtctccat cacatggaac ggctcgacct
     5401 gaccgcgccg tgacaatcgg gtcatttgcg gcgcagcttc gctagtgccc ccaccagcag
     5461 cgcgcccaga ccgagcccgg tcgcggagcc gacgatatag ccggcgccga ggccgacgcc
     5521 catcgatttg cgcgccactt ccgccgagac cacttcctgg ccgggcttgg tgggcagcga
     5581 ccgcagcgtc ggctcggtca ccacaagctc gggtagaacc tcaggctcgg gttcctcagc
     5641 cgtggccagc cacgccgcga tcatcaggat caaggtcgcg aacaggttca ggaacagcat
     5701 cagcaccagc gtccaggtga acacggcagt gccgaggtta cgcgagaacg caccgatcag
     5761 gatgccggtg aacgactgca gaatcaggag tccggcggac ccgatgccag cgcccaccca
     5821 ggccagatgc gagggcactg ggtgcgggct gaaccagccg aagagcagcc ggaacagcac
     5881 cgcgccggcc gccagcgaca ccagcgcact caccagcctt agcacgacgg tccagagcag
     5941 gctgttggag atccccagca ggtcgcccag cgcgccaccg aagcccgacg cgatcgcaga
     6001 cgccacgaac gtggccgcca ctccgaccag cacccccacc agaccggcga agttcgccag
     6061 cacatcgagg ggcaacggta gcgtccgtcc cggattgtct acctcggcgc gcatcagcag
     6121 ccggatggcg cgcttgaggt tgccgatcca gttggaaccg acccacatca tgatgcccat
     6181 tgcaaccagg ccggtggacg cccaactggt cagtgcgcgc tcgatcacct cgtggatgtt
     6241 tcggccgaag tcggagtcgg tgtcgagttg cccgttgatc caggtctgaa tcaccccgag
     6301 cgcctccggc atgaagaccg tcaaggtgaa tccgagcgcc gaaaacgcca gcatcaggat
     6361 cggtaccagg gacaacaccg agaagaacgc gatcgcggcc gccaactggc cgccgccacg
     6421 cacgttgaac cgctccaccg tccgtaggat gtgcgccacg aacgggcgct tcgagaattc
     6481 cgcccaccac gccagcgctg cttccaccac tgtctactcc gacgcgtagt ccgcgaccgg
     6541 cgcgcaggta cagaccaggt tgcggtcgcc gtaggcctgg tcgatccggg caaccggcgg
     6601 ccagtacttg tcgcgtccgc cggagccgat cggcccccgg cgcacaccgg cgggataggc
     6661 ggcgagttcg cgggagtacg gatgtgtcca ctcgtcggcg aggatccgag acaccgggtg
     6721 cggggcgttg tgcaaggggt tgtcgtccgc cggccacaca ccggcggcca ccgcgtccat
     6781 ctctgcacgg atcgcgatca tcgcgtcgca gaaacggtcg atctccgcct tggcttcaga
     6841 ctcggtgggt tcgatcatca gtgtcccggc taccgggaag ctcatcgtgg gcgcgtggaa
     6901 gccgtaatcg accagtcgct tggcgacgtc gtcgacggtg atgccggtct gcttggtcaa
     6961 ctcgcgcagg tcgatgatgc actcgtgcgc caccagtccg taggccccgg tgtacagcac
     7021 cgggaagtgc tcgcgcaggc gtcgggccac gtagttcgcc gccaggatgg ccgactggga
     7081 cgcctcgcgc agcccgtccg cgcccatcag ggcgatgaag gcccaggtga tcggcaggac
     7141 tccggccgag ccgaatggcg cggcgctgat cggtccatag ctgttcgccg ggccggcgct
     7201 cggctcgagc gggtggttcg gcaggtaggg cgccagatgg gcgcgtaccg ccaccgggcc
     7261 gacgcccgga ccgccaccac cgtgcgggat ggcgaacgtc ttgtggaggt tgaggtggct
     7321 cacgtccgcg ccgaacttgc cgggacgcgc cagcccgagc attgcgttca ggttggcacc
     7381 gtcgacgtag acctggccac cggcggcgtg gacgcggtcg caaacctcgg tgatggtctc
     7441 ctcaaagacg ccgtgggtgg acggataggt gaccatgacc gcggccaact cggcggcgtg
     7501 cttgtcaatc ttcacagcca ggtcggtcag gtcgaccgag ccgtcggctg ccgtggcgac
     7561 caccaccacc cgcatgccgg ccatcaccgc ggacgcggcg ttggtgccgt gcgcggacgc
     7621 cggaatcagg cagaccaccc gatgcggttc gccgttggcc aagtgatagg aacggatcgc
     7681 catcaggccg gcgaactccc cctggctacc cgcgttgggt tgcaggctca cccggtcgta
     7741 gccggtgatc tcggccagcc agtcgcagag ttcgccgatc atccgagcgt agccctgggc
     7801 ggccgcctcg ggcacgaacg gatgcaggcc ggcgaagccc gggtagctga tcggctccat
     7861 ggccgccgca gggttcagct tcagcgtgca cgaccccagc gggatcatgc ccttgtcaag
     7921 cgcgaagtcc cggtcggcga gcgtccgcag gtagcgcatg aactcggtct cgctccggta
     7981 cgcactgaac accggatgcg tcaagaacgg ctcggtgcgc tcgtggtcgc ccaacccgcc
     8041 gaatctggac gcgttgggcg gcgccacccc gaaggcttcg cagaccttca ccagggcagc
     8101 ctcgtcggtg tcctcaccga ggctgatccc gacctggtcg gaatcgctgc gccgaaggtg
     8161 gatcccctgg tcgcgggcac tttccacgac gtcgtccgcg cgccccggca cggtcacggt
     8221 gagggtgtcg aagaaggcgt ccgaggccaa ctccagcccc ccggcggcaa gtgctgcggc
     8281 gagtcgacgc gtccgctcgt ggacggatcg ggcaatcgca gtgagccctt ccggcccgtg
     8341 gtgcacggcg taggcgctcg cggtgaccgc gaggagaacc tgcgcggtgc agatgttcga
     8401 ggtggccttc tcacgacgga tgtgctgctc acgggtctgc agagccagcc ggaaagcggg
     8461 gatgccgtcc gcgtccttac tgatgccgac gatgcgtccg gggagctggc gttccaagcc
     8521 ctcgcgaacc gcgatgaagc cggcgtgcgg gccgccgtag aacagcggga cgccgaagcg
     8581 ctgcgctgag ccgaccgcga tgtccacgcc ccaactcgct gccgaaccga tcagcgtcag
     8641 cgcgagcagg tcgacggcgg cgatgagctg agcgccgttg tcgtgcgcga gctgcgccaa
     8701 ggctcccagt tcggtggcgg acggcagcac gccgtccgcc ccaggtacct ggaccaccac
     8761 ggcgaagcac tggtgcgcgc gaaccgaatc ggccacggaa tcgtcggcca ccacgatgtc
     8821 gatgccggtg gccagacagc gggtgcgcag caccgcgagg ctctggggca gcagctgtgg
     8881 atccaccact acgcaaccgc ccttgcgggt cacccggtgc gctactgcga ccgcctcggc
     8941 gaccgcggtg ccctcgtcga gcaggctggc cccggtgatc ggcagtccgg tgaggtcggc
     9001 gatcacggtc tggaaattcg ccagcgcctc caggcgaccc tggctgatct ccggctggta
     9061 gggcgtgtag gccgtgtacc acgccgggtt ctccaggatc atccggcgga tcaccggcgg
     9121 ggtgaccgtg ccgtggtagc ccaggccgat catcgacagc cccggacggt tctgcgcagc
     9181 catcgcggca agctcgcgct gcacctcgaa ctcgctgcgc ggctcaggca agtcgagacg
     9241 ctcacggagg ctgatggcga ccggcactgc ggcgtcggtc agctcgtcca ggctggcgta
     9301 gccgagcgcg gcgagcattt cgaaacggtc ggacgcaacc gcgcccaggt ggcgttgggt
     9361 gaagtcattc atcgcaggca ccaacctatc ctgctatgca cctcgggtcc ggtaggccga
     9421 gctttgctgc cctgcttggc gagacccgtg ggcgccggct cagccgaccg cgcgcgcctg
     9481 acggcggcgc gacagttcgt cgtcagccgg ctgcgggtga tcctcggcgc gctcggcggg
     9541 caactgctgc agagtgcctt cgatgtcccg ccagacgccg cccagcgcga tgccgaacat
     9601 gccctggcca ccgcgcagca ggtcgaccac ttgatcatcc gtcgtgcatt cgtagaccga
     9661 aacgccgtcg ctcatcaggg tgacctcggt caggtcctgc actccgcgcg cccgcaggtg
     9721 gtcgatagcc gtgcggatct gctgcagcga gatgccggcg tcgatcagtc gcttgatcac
     9781 cttcagaatc agcaggtcac ggaagctgta cagccgttgc gtcccagatc ccccggctgg
     9841 ccggacctcc ggcaccacca gaccagtgcg tgcccagtag tccagttggc ggtaggtgat
     9901 gtcggcggcg cggcaggcca ccggcccgcg gaagccgaga tcgtcgggca gcggcgagaa
     9961 gtcgccatcg aacagcagat cctgtgccgg gggcacctgg ttcattgcgt tcatcggtcg
    10021 gcctcgtttc gctggaatca ccgcgttggg attctgacgc taacctcgcg accgcctcgg
    10081 gtcaacgaca cgcccggacc ggcgttcacc tatccagcga gtcttaacca cttcctcagg
    10141 gtttgccttc atcgccggtg aagtcctccg gggtcaccga gtcgaggaac tccaggaagc
    10201 gttccacctc gtcttccggg gtttctacgc catcagccgg ggccgtgccg agttcctccg
    10261 cgctcacgcc taccgtctcc agcagctcct cgctgcactg gatcgggcga ccggagcgaa
    10321 gtgcgatggc gatcgcatca ctcggccgcg ccggcagcac cgtctcgccg aagacgagtt
    10381 cggcgtaata ctgcccggcc tcgtaccggg tgatcgtcac cgcgctcagc ggttcactgg
    10441 agcttgtcgc cagccgccag gcgacgtcgt gaatgctcgg acgctgatcg tccggtggct
    10501 cggtggcgct gaagatcgcc gaggctcccc cgtggctcat ccagatcgga atccggcgct
    10561 cagcaccgcc cacttcggcg agcacaacta gcggcggcgc ctcgcccgtc gacacccgga
    10621 tctcgatgac tttcagctcg cgcatggctg gcctcactgc ggtaactggg atctcatgat
    10681 cgcggcatgg gcgtgcagca ccagctgcat cacgtcggcc ggcagggtgc gagcggacgg
    10741 gttccggcgc aggtgcggcg ccacggcctg ctcgatcagg ccgacctcgc gttcagcggc
    10801 ctgcttgacc acgcgcaggt gacgcgcgtc catgccgaag gcggcgagct tacgagcgat
    10861 caccgcaacg gtgagcgcat cgcgcccgta gtagatggtg ccccggcgcg gcaccacaag
    10921 ttggtgcttc tccaggtcgg agagcgccgc ctccgccagg ccgctcagtt ccaacaactc
    10981 acgtcgggtg atccggatgg cgcgcttcgg tgcgtgggcc tgaccgctga tcgcggccgg
    11041 ggattcgacc ggcgtcgcgc cctccgccgg gcggttggta ccgaggaccg gtggctcctg
    11101 cccgcggtcc atcatgtcca ggtgctcgcg gatcaccttc aacggtaggt agtggttctt
    11161 ctgcacatcg aggatgtagc gcaaccgctc gatatcggat tcggcatagc gccggtagcc
    11221 cgaaggggcc cgctccggcg agatcaaccc ttccgattcg aggaagcgga tcttcgagat
    11281 ggacacgtcc tcgaactcgg gcttcaggac agccagcacc tggccgatgc tccgcagccc
    11341 gatcgccatc agtcgtgccc agtgaagaac accatgcgga acttgccgat ctgcacctcg
    11401 tccccagtct tgaggaggac atccgactcg accagcacct tgttcacgta ggtgccgttc
    11461 aagctgttct ggtcgctcac ccagaactcc gaaccgcgcc gcacaaatcg ggcatggtgc
    11521 cgcgagacgg tgatgtcatc caggaagatg tcgcagcgcg gatgccggcc cgaggtgacg
    11581 acgtcggagt cgatcaggta gcgcgcgccg gcgtgcgtgc cgcggagcac cagcaggatc
    11641 gccgagcccg gcggcagcgc ctcgacggcc gcggagtcgg cgggcgtcag gtcttcagtc
    11701 tggaactcgt cgaccactgc ggggataacc ctggtggtgt caccggagag ctttgccagg
    11761 cttgcgccgc agtgcgggca gaagttcacg ggctcgacga gttcaaagcc gcatgcgggg
    11821 caagtgctca tcacgttcac ctttcgtgcc tggctggtcg gccgtgggag ggcaggctca
    11881 accgcaggtt gacgtcaagc ctatcccgac accagctcgg cgtagccgtc tgcgtccagc
    11941 agttcgtcca gctcttccgg atcgctcaac tcgagctcga agatccaacc gtcaccgtaa
    12001 gggtcagagt tgatggtctc tggggtgtcg ttgaggagtt cgttcacggc ggtgaccacg
    12061 ccggtggccg gcgagtacac gtcggagaca ctcttggtcg actcgacctc ggcgcaggcg
    12121 tcatcgacgg cgatctcttc gcccacctga ggcagtgaga cgtagacgac gtcgccaagg
    12181 gcttcggctg cgtagctggt gatgccgatc cgaaccgtgc cgcccgcgcc ctgccgcacc
    12241 cattcgtgct tgctgctgta ctgaagatca tccgggtaca tcgtggttcg actccttctg
    12301 gcagcaggtg ccgccgctca cttcgggcac caccctagcg ccctcgggcg ccgctcagct
    12361 ggctgaattg tcaccggttc cgacggggat ccgccgaatc acgaccgcac cctggctgag
    12421 gtagagcagg ccggaccacc agtagaggcc cgcgccccag atcacgcacg cccagccgag
    12481 gacgcgggcg gtggtcggca tcagccagtc accagcgccg agaagcacca ggggcaagcc
    12541 gtagagcagc atgaacgtgg cggccttgcc gaggaagtgc accggcaggc tggcgaaacc
    12601 tcggcgtcgg agcgccgggc cgatggtcac caccatcaac acgtcccgag ccgccagcag
    12661 tacgaccagc caccacggca cgatctcgcg aatcgcgagg cccagcagcg tcgccaggat
    12721 gtacagccga tctgccaccg ggtcgagcac ttgtccgagg cgcgagcgtt ggtgccagag
    12781 ccgcgcgagg tggccgtcca gccagtcggt ggccccgccg atcgccagca cgaccaccgc
    12841 ccagacatcg gcttcgcgga tcagcaggag ccacaggaat agcggcacgc ccagcagccg
    12901 aatgaagctg aggatattgg ggacggtcag cacccgctcg gtgtcatagg gttctccggc
    12961 cacggccaca ggatagcgcc caagcgccga gagccagcct ttggaaagcg ctgatcgatg
    13021 agcgttggcg ctggctggtg cttgtcggag ccgccactgg ccgcggccgg gctacgcgtc
    13081 ggagtcggcc tgctgggctc cgggacgcaa cgaacccgct tccggcgggt tatgacgacc
    13141 atcccgtgcc cttggcggag caaacccgag tttgacgagt tatcgggcca agatccggct
    13201 ctataaccgg tcgaagtcgg gtcgactatt acccaagccg ctggatgggg cccgatagcc
    13261 agccgaaacc gggttatatc gaccaacagg tgtggttcca cctgtccgaa gagcggcggc
    13321 ggaccagatt aacgttgtgg agaagcttca ttcaggtgag gcgatgtttg cggcgttgaa
    13381 ccggctgttc gaggccaacg ggcatggatt caactctggc cggggcccgc ggcgttaggg
    13441 ctggccatcg gcgcttgacg tggagttgcg ccgccagagg tggatccggg ctcaggccca
    13501 ggcggacgcg agttggtcgc gggtgtcggc cagcagttgg ggcagcgcct tggtctgggc
    13561 gatcaccggc aggaagttgg cgtccccggg ccagcgcggc acgacgtgct ggtgcaggtg
    13621 cgcggcgatg ccggcgccgg cgacctgtcc ctggttcagg ccaaggttga agccggccgg
    13681 cttcgaaacc cggcggatca cggtcatcgc cgcggcggtg aactcggcaa gctccagcag
    13741 ttcttccgca gtcaggtcgg tgtagtcgct gacgtgacgg tacgggcaga tcagcaggtg
    13801 gccgggcgag tacggataga ggttcagcac cgcgtagacc gtgcggccgc gacgcaccac
    13861 caggccgtcc gcgtcctcac gctcgggcgc gcgacagaac ggacacgaat gcgaggcgcc
    13921 atcgacgggc ttgttctcac cctcgatgta gaccatgcga tgcggggtcc aaaggcgctc
    13981 gaacgcgtcg gggacgccgg ccagggtgcc ggcgtcctcg accacttcgc gggcttcccc
    14041 gggctcggtc atcgccgcgg cggccgggtt gctgcgatga tctccgcgac cgcctctgcg
    14101 atcggcacgc cgttgcgctg gctaccgtcg cggtaacgga aggagaccgc gccggcggcc
    14161 acgtccgtct cgccggcgat cagcatgaac ggcaccttct gggtctgcgc gttacggatc
    14221 ttcttgccga agcgatcatc gctgcggtcc acctcgaccc ggatgtccgc tgcgatcaac
    14281 tgcttggcca cctcggaaag gtagccctcg tactcctcgg ccaccggaat cgccaccacc
    14341 tgcaccggcg ccagccacgc cgggaaggcg cccgcgtagt gctcggtgag cacgccgatg
    14401 aaccgctcga tcgaaccgaa ctttgccgag tggatcatca ccggctcggc cggatgcccg
    14461 tccggtccgg tgtaggtgag cccgaagcgg gccggctggt tgaagtcgta ctggatggtc
    14521 gacatctgcc aggtgcggcc gatcgcgtcc tttgcctgca ccgagatctt cggaccgtag
    14581 tacgcagcgc cgcccggatc gggcaccagc ttcagccccg actcgatacc gaccttctcc
    14641 agcaccgcgg tggccttctc ccactgctcg tccgagccga tgaacttggt cgacttcgcg
    14701 ccctcggtgt cgcgggtgga cagctcgagg tagtagtcgt ccaggccgaa gtcctccaac
    14761 aggttggtga cgaaggtgag caggtgcttg acctcttccg ctgcctggga cgccatcacg
    14821 tatgagtgcg agtcgtcctg ggtgatgctg cgcacccggg tgaggccctg gagcacgccc
    14881 gacttctcat tgcggtacac ggtgccgaac tcaaagaagc gcagcggcaa ctcgcggtag
    14941 gaccgtccgc gcgaagcgaa gatcaggttg tgcatcgggc agttcatggc cttgaggtag
    15001 tagttctggc cctcgtcgtc catgggcggg aacatgccgt ccgcgaaaaa gggcaggtgg
    15061 ccggacgtgc ggaacagcac ctccttggcg atgtgcgggg tgcccacgta ctggaagccc
    15121 gagcggatgt gcgcagtccg gacgtagtcc tccatgacgc gcttgatcac gccgcccttg
    15181 ggatggaaca cgggcaggcc ggcgcccagt tcctccggga agctgaacag gtcgagttct
    15241 gcacccagct tgcggtggtc gcgacgcgcg gcctcctcaa gccgggtgcg gtaggccacc
    15301 aggtcgtcct tggacgccca cgcggtgccg tagacccgct gcagctggtc gttgcgctgg
    15361 tcgccccgcc agtaggcggc cgagctgcgg gtgagcgccc aaccgttgcc gagatagccg
    15421 gtgtgcggca cgtgcggccc gcggcagagg tccttccagg cgaccgcgcc gtcacggcgg
    15481 acgttgtcgt agatggtcag ttcgccggcg ccgacttcga ccgaagcacc ttcggtgttc
    15541 gccgcagcgc ccttcagccc gatcagttcc agcttgtacg gctcggacgc gagctcggct
    15601 gcggcggctt cgtcactgat cggccggcgg acgaactgct gccgctcctt gacgatgccg
    15661 accatggcct tctcgatggc cttcaggtcg tccggcgtga acggggtctc gacgcggaag
    15721 tcgtagtaga agccgtcggt gatcggcgga ccgatgccca gcttcgcgtc cgcgtgcagc
    15781 agctggacgg cctgcgccgc gacgtgcgcg caagagtgcc ggatgatcgc caacccctcg
    15841 tcgctggtgg ccagcaccgg ctcgacctgg tcaccctccc gaagctcggt ggcgaggtcg
    15901 accacggcac cgttgacccg ggccgcgacg atggaacgat cggtgccgaa caggtccagg
    15961 gccgtggtgc cggtggtcac cgcctgctcg ctcggttgag cctgcggatc gatgacgatg
    16021 atggacacgg acactggatg gcttccttcc ttcggataca gccctcctag tctgccgcag
    16081 atttcggccc gtccgtgccg acgtcaccgc tcgcgcgaca ccaccggccc gactgccaac
    16141 gaatcgatga tccggtcggc gtagccttcc agctcgcccg agacgcggac gcggatcagc
    16201 ttgcccgcct cggcgccgct gagcacgacg gtgtccccca tcgcccgggt gagcaggacg
    16261 ccgtcggcgt accagcgcac ggtggtgcgc acccccgctg ggccccatac gccggggtga
    16321 accgtcagcg tctttccgac cagcgcgagg ccctcaatca ccggcagatc cgggatgagg
    16381 tctcccggct cgatgacatt ggtgtagccg gagtacttgt agaccttgtc ggggtagccg
    16441 cgtacgcggc ttgatcactt tgccgaggtc ggccagggtc agctggtagc tcccgctggt
    16501 ggcgccgggg atcttgtcgg ccccgcgata acactgccac cgctggtcga cctcgcccgg
    16561 accccccgcg tcgaccgagc cggtcagggt tcggcccacc ctggccgagc ctctgatcaa
    16621 cggggtggcc attaccaggc gtcccggctc gatcgggcct acggtcgctg tctcctgagc
    16681 caacttcggg aagccggcct tgtagccgct gatccggagc gagaccttgt ggccgatgtc
    16741 ggccgcggcc aagcggtagc tgagctcgct cgcaccggag atcggcttgc cgtcacgcaa
    16801 ccagcgatac tcctgctcca cccgtcccgg gccccagtcc ggctcctcga tgctcagtac
    16861 ggagcgatac cggggagtgc ctccaatctc cggcaccgcg gacggaacga gtgccggcgg
    16921 tgcggtcggc gtggatcccg gcggggattg catctgatct cgggttgcgc caccgaaaga
    16981 gctggccatg tcgggcctct ctagagctgt tgaaggcaac gccagtgttc acaggccaag
    17041 cgtggtgacg atcgcgccaa attcgtggga accttttgtc atgacaaccg atcatatgcg
    17101 gctgttcccc gggaagtttt tcatcagcac cgcacttgag gcgctggaga ccaagaccgc
    17161 gatgtccaag gagctggccg tccgctacct ccagcgagct gccatcgccg ggctgctgat
    17221 cgccatcttc tacctcgccc acttcggcgc agtggccgcc ttcggctcgg tccaggtcgg
    17281 gacggggagc ctggcggtgc tgggcaagat ggtcggggcc gtgatcttcg gctgggccct
    17341 ggtcttcatc tatttcacta agtccgaact cctcacctcc aacatgatgg tggcctcgat
    17401 cggcatctac taccgcaggg tgaagtgggg ccgcgcactg cggatgctgg gcctctgtta
    17461 cgtcgggaac ttcgtgggcg ggctggtcct ggcagccctg gccaggttga gcaccctggc
    17521 cagcggcgcc gtcctgagcg agatggagca ctcggtgacg gtgaagcttg gctacatcag
    17581 cgcgggcccg ctgggctgga gcgatctgct catgcgcgcg atcctctgca acctgatgat
    17641 caacgtcgcg atgctgctgg tctacaacgg cttcatcaag gatgacctga ccaagagcct
    17701 ggccatgatc atggctgtgt tcatcttcgt gttcctcggt ttggagcact cggtggccaa
    17761 cacgattctg ttcacgatcg tgggtttgaa atcgggtatc gatctggccc ccgccgccgg
    17821 taacgtggcc atcgccctga tcggtaactt catcggcggc ggcgtcctga tcgggttgta
    17881 ctacgcgtac gtgaacgacg attcgaagta ccgggccaac ccctgagagc ttgaggagat
    17941 ttcggtggcc gacaagccca agtggtggtt tcgcaagccc gcgctcaact ggctggaact
    18001 actcggaatc tcgctggccc tggcgctgat cggtggcgcg ttgggcgtaa ctgccgcgct
    18061 caagctctcg ccggagcctg gcgcctgcga ttcggtgcgg gtggcggaca gcgtgctgcc
    18121 ttcggtggtg accatcttcg tgacgggggc atccggcggc ggatccggca gcggcgcgat
    18181 catccgggcg gacggcctga tcctcaccaa tgaccatgtg attgcggccg gagtcgagtc
    18241 cgggaacatc gaagtgcttc tcaacggcgg cgagcaggtg ccggccgatc tgatcggcac
    18301 cgatccgatc accgatctgg cgctgctcaa gatcgacaag aacaagctgc ccacactgct
    18361 gctcgctccg cgcgagccgc tgagtgtggg tcagccggtg gtcgcgctgg gcgcgcctct
    18421 ggggctttcg gggaccgtga ccagcggcat tgtcagcgcg ctgaaccgga acgtgccggt
    18481 gccgaaggcg agcggtggta ccacggtgct aaccggcgcg atccaaaccg acgcggccat
    18541 caacccgggc aactctggcg gcccgctggt cacctgcgag ggacgcctgg tcggcgtgaa
    18601 cacggcgatc tcgaccgtgc cgaacgcaag tggcgtcgcc ggcggcggca gtgtcggcat
    18661 cgggttcgcg gtgccggcga gcactgcgca gcggatcgtg gacgaactgc tggcgaccgg
    18721 gcgcgccacc catccgtgga tcggcgcggt cactgccgag atcacccagg acatggccga
    18781 ccgcttcggc actccgtccg gactgttcgt gcaggacgtg agcgaaggcg gcccggcgga
    18841 cgcggccggc ctggaagccg gcgacatcat cacccgcctc agcggcaacc cggcgacgag
    18901 cctcggactg ggctggctgc tggtgagcgc caaggttggc gacgacatcc cgatcgagtt
    18961 cgcccgcgac ggcgtcttgc agaacgcgac cctgaccctg gccgagcagc ccgcctagcc
    19021 cggcagcccc actggcccgg caccgaggca ccccgcgtca gccctctttc gccacttcgg
    19081 acggcggcgt ggtgctagcc ccgggcgggc ggaagttggg cgcgcagctg gtgaagcacc
    19141 tcaggtccgc cgatagcttc gatgacagca gggtcgccga caaccacgag ccggtcggtg
    19201 gctcgggaga gcccgacgta gaggcgctcc cggcttcggt cgcgggttcc gtcctcgttc
    19261 acgcagagga cgacggcgtg ccgttccatg cccttgaacc cgagaacatg cccgtagaac
    19321 acgtcgtcgt tcgaccagaa ttcgtcccaa tagccggcaa agccgagcgt ttcctgacgc
    19381 tccttctgca ccgggtggcg tgcgcccatc gtgagcagcg ctacatcttt gggttcccaa
    19441 ccttcctcaa gcaggcgctc gatctggtcg tcggccgctt cgatcgcggc gtgcgtaggc
    19501 accgccacga actccacctg cggaccctct cccccacgca ggaccattga ggttggcgcg
    19561 agcgggacga agctctgcgc gatctgtcgg gtgttgcgaa ggttgtggtc caggaccagc
    19621 ggcaccaggg ccaacgaggg gcgtccgaac cgcgcgaaga cgcgctgccg ctcgtcgctg
    19681 taggcgaaga ccccgccggc ggtctcgtcc ttcagggccc cgacgagcgg cgtccaccag
    19741 tcctcggcga aatcctgcgc ctcgtcgacg atcactgcat cgaatcgcga ttccggcgga
    19801 agttcggcgg cgcgggcggc catgagcgcc ggcagttcga cctcccagaa gtcggaatcg
    19861 tcccgggatc cggtgatctc aatgccccac cggcgggcca ggtcctcgaa cgtcccgacg
    19921 aaagccggac gcttggcact cgatccggtt agcagtgacc gctgcaggtg gtgggccagg
    19981 ccgtagctgt agcagaccac cgccactcgc tgccgctcgc cggtggcccg gccaccggcc
    20041 aggtcggacg cctgccgaat ggccaagacg gtcttgccgc tgccggcgcc gccgcggatc
    20101 tcgacccggt tcagtaggcg ggtgacgctc agcagctgag cctgttccat cgtcagccgg
    20161 tcagcgatgg acgcccggtc ctcggcgatc gcgaccacgt cccgccccgg taggtcgcga
    20221 ccgatcagga tctcgcggat caggtccacg tcgtcacggg tcggcggcct ggcgtccgc
//
LOCUS       DAOPRQ010000048        19933 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_2301_length_19933_cov_11.248617, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000048 DAOPRQ010000000
VERSION     DAOPRQ010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 19933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19933
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2301_length_19933_cov_11.248617"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..692)
                     /gene="raiA"
                     /locus_tag="PKJ61_07605"
     CDS             complement(66..692)
                     /gene="raiA"
                     /locus_tag="PKJ61_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016456203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOC13729.1"
                     /translation="MDVLVTGRHCQIPDEFRARVEERIASIEKLKDRVIRVEVQVSAY
                     GYKKQPDQSTRVEITLRSRGPVVRAEASADDKNVAFDHALDRLKSQLRRASDRRKTHR
                     GLRGTGSLADAALAVPAESAEETRPEIRKIAGLEIHGDGPLVVREKYFDAVPLTLAQA
                     LDEMELVGHDFFLYVDAESGHPSAVYRRKAYDYGVIHLNLDAVEEATA"
     gene            999..2363
                     /locus_tag="PKJ61_07610"
     CDS             999..2363
                     /locus_tag="PKJ61_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013161879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HOC13730.1"
                     /translation="MILELNSGDTAWILISAALVLLMTPGLALFYGGMVRAKGVLNMM
                     MMSFISMGVIGVLWVLFGYSLAFGNSVGGAGIIGNPFEFAGLQSLLVNDPEASYPALA
                     FVAFQACFAIITVALVSGAIADRASFGAWTAFAVLWGTLVYFPAAHWVFAFSNPDAGV
                     VGGWIANVVGAIDFAGGTAIHINAGTAALVLALVIGPRIGFGTRPMRPHNMTLVMLGA
                     ALLWFGWFGFNAGSAVAANGLASLAWVNTLAATAAAMLGWLVVEKLRDGHPTSLGAAS
                     GIVAGLVAITPAAGAVSPLGSLAIGALAGIACAYAVGLKYRFGFDDSLDVVGVHLVGG
                     LVGTLLIGFFADPATSGDPADPGVAGLFYGGGLDQLGKQAIGAFAVMAFSFVMTYLIA
                     LAIKYTIGWRISSADELSGIDLAEHSEAGYDLSPVYYTNKVQRTLVLTKDDMPVAETT
                     GAKK"
     gene            2360..2698
                     /locus_tag="PKJ61_07615"
     CDS             2360..2698
                     /locus_tag="PKJ61_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HOC13731.1"
                     /translation="MKLVTAIIQPEMLETVQKALVRHGVGGMTVSEVSGYGRQRGHIE
                     VYRGEEYTIDFITKVRLEVLVDDEDAEALTGVIVQAARTGEVGDGKVWIIPVDTVVRV
                     RTGESGSTAI"
     gene            complement(2843..3529)
                     /locus_tag="PKJ61_07620"
     CDS             complement(2843..3529)
                     /locus_tag="PKJ61_07620"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HOC13732.1"
                     /translation="MTIARLLSVGGELVLGAHCPGCGASGLGVCAGCRTAIAVRPVAV
                     AGLPPGLPAVVAGGRYDGELRRLLLAAKERDALGAVPVLAERLAVALARLLLDRPELA
                     AGGLLIVPVPTARAAAAERGLDLTLALARLAARRLRGTGLDVRASAGLRQVRRPADQA
                     DLGREGRLANLAGAFTAGRLPPGELLLVDDIVTTGATLVEAVRALRAAGREPFAVATV
                     AATTRRITPS"
     gene            complement(3578..5302)
                     /locus_tag="PKJ61_07625"
     CDS             complement(3578..5302)
                     /locus_tag="PKJ61_07625"
                     /inference="COORDINATES: protein motif:HMM:NF022113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpqB family beta-propeller domain-containing
                     protein"
                     /protein_id="HOC13733.1"
                     /translation="MPNRLLALVVAVLLALGGCATVPTSGPIEHHTQQVPGTDSGVSV
                     HPLPPATGASQLLVVEGFLHAMSIHQPNYAVARQYLTETASALWHPESGVQVYADGKP
                     PEEFGQTVMLITPLVGRLDSLGSFATASEQLRHDFELVRDANNQWRISNPPDGLLVSR
                     YLFSTNFTPVTVHFLDASGSVLVPEQRYFANGDQALTAAVQAVLSGPSERLAPALRRA
                     SVSELDVDNVSLDERGVTMVELGSDGLRLTTEERQNLLAEIVNTVVGFAQVTAVQVSI
                     GGLAIAGEFGGTELDGDDFTRMSPDNVTAQRSLFAIAEGRVVALREANWADFSPVEAD
                     LTRPELIAVRSDLAEVAAITDSATRLVLAPVGAAKSRTVRTGTGLLRPDFARNGELWS
                     ATASGPGSFRVFRDGLTIRVDGSELPKRPLVASKLSPDGTRIALVLRNGTRTEVGVAV
                     VVRTDDQIRLTGWRPLEVNLSTGTDGAALDLGWASRSTSTKWSPAEVAVLQRLETGDT
                     SVVRVSEDGATATDIGPTKAASLIKLAVVPGRPAVALTDSGAGYRFESEFNWALAVTA
                     VDDLAYSG"
     gene            complement(5295..6848)
                     /gene="mtrB"
                     /locus_tag="PKJ61_07630"
     CDS             complement(5295..6848)
                     /gene="mtrB"
                     /locus_tag="PKJ61_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015070721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MtrAB system histidine kinase MtrB"
                     /protein_id="HOC13734.1"
                     /translation="MSRLSSNPALRLWSRSLPFQVVVTTLAAAIVILAATGWFLMEQS
                     SRGIMAGKTQASVAEASAVVSNMQRDLSTTDLRTTSASERITRLAREATNRGLAGNQY
                     FVVVETPIQKIGTGGLAPESIPDSIRESVGSGDGLWSTPTLIRFTDSRPDEPGLVVAA
                     TLQAPGQAPYPIFFLFSTSQEKATLAVVQQATLATGVFLLAGLTLTVYLIALQVLRPV
                     RAARLAAEQLASGHLEDRMAVVGTEDLAGLARSMNHMAEELDKKITQLQNLSLVQQRF
                     VSDVSHELRTPLTTIRMAAEVLHSHRDEFDAVSRRSTELLSAELDRFEGLLTDLLEIS
                     RFDAGAAQLALDYVDLTVLAGEEMAAQQRLAERTHTQLTLDSGGNCVAEVDGRRIRRV
                     LRNLITNAIEHGEGRPITVYVRGDENAVAVAVRDHGVGFTAEEGSQVFNRFWRADPSR
                     GRHVGGTGLGLSISLEDARLHGGRLDAWGSPGAGAQFRLTVPRKAAEELQGSPWPSVP
                     PDAKVVLHA"
     gene            complement(6845..7576)
                     /gene="mtrA"
                     /locus_tag="PKJ61_07635"
     CDS             complement(6845..7576)
                     /gene="mtrA"
                     /locus_tag="PKJ61_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MtrAB system response regulator MtrA"
                     /protein_id="HOC13735.1"
                     /translation="MEGNKVSEAPRSAPPRILVVDDDEALAEMLHLVLGSEGFDAKLC
                     HTGDAALEAFREYRPDLVLLDLMLPGRDGVDVCRDIRAESGVSIVMLTAKSDTIDVVD
                     GLSAGADDYVAKPFKTQELIARIKTRLRRHITPEPDVDNLRIGDLVIRVNEHTVRRGE
                     NEIILTPLEFDLLLALARRPRQAFTREVLLEEVWGYRHASDTRLVNVHVQRLRSKIEK
                     DPEKPEIIVTVRGVGYRAGEPRTPV"
     gene            complement(7617..8174)
                     /locus_tag="PKJ61_07640"
     CDS             complement(7617..8174)
                     /locus_tag="PKJ61_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HOC13736.1"
                     /translation="MTGFRAGDLLVAAVTITDGVFDGAVVLLLDADDTGTVGVVLNRT
                     TSIELSEALPAWAELVCEPPALFDGGPVSQQGAVCLAEPWALGEEPPGWRPVFDGVGL
                     LNLDTPVEIAEGTYQHLRIFAGYAGWDAGQLERELDFEMWHVVRATVTDVFDPDPATL
                     WRRILRRQGGELALFSTWTAETELN"
     gene            complement(8275..8400)
                     /locus_tag="PKJ61_07645"
     CDS             complement(8275..8400)
                     /locus_tag="PKJ61_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13737.1"
                     /translation="MAVAIEAIRGFLHLFTEDWSQHDGSPFHVITVLDVLEPKHS"
     gene            8558..9196
                     /locus_tag="PKJ61_07650"
     CDS             8558..9196
                     /locus_tag="PKJ61_07650"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HOC13738.1"
                     /translation="MTAYLPRDISLVGHCVGLAPLREADLPDLAPILMDPAVYASGYV
                     MHRRPATTEDALALGRSRFLAADAPNGVGFGRIAFAVRLATDSPLDAAGSLVGTTSLG
                     DAHLANESIHLGWTIYGSRWWGTAVNAETKYLLLRHCFEELGFGRVKLQTDRLNVRSA
                     AAIAKLGATREGVLRRETRREDGSFRDTVVFSVLADEWPAVSAGLLARLNAA"
     gene            complement(9161..10687)
                     /locus_tag="PKJ61_07655"
     CDS             complement(9161..10687)
                     /locus_tag="PKJ61_07655"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HOC13739.1"
                     /translation="MKMDTAATLARLTELRAHDAPTHGGRLLSYVYDSGHPELDELAA
                     AAIRAMQPVNGLDPTAFSSVAVIERELLDFAREVFHAGPDVAGTATSGGTESCLLAVK
                     TARDSWRANGGTERPRLVAPTTMHAAFRKAAALFDLDLDLVPVDVTTGRVDAGVMAER
                     FSPDVALAVVSAPCYPFAALDPVAEVAEAAQLAGVDLHVDACIGGFALAFWPPSDAAA
                     PGGAWDFAVPGVTSLSADLHKYGYAPKGISVLLQRGFDRQRHQWFATSSWPGYPVVNA
                     TLLGSKSAGPLAAAWAIASVLGVGGFRQLTASMAATTARLSAAVGAIDGLSVVGAPAG
                     PMLSLAADVTDPARQVDPHLWADAMRTHGFVVQGQPASTQPDGTVLPATAHLTITPVT
                     AHVVDELAAAMAAAADTVRGRPHLDGTAVAAGLMGGADPAALAGLDADAAFAVLSGAG
                     LLEPGALHAMAPVLALVEILPASVVERLLIELLASPLDRPGSGPTPGSGGVEAGQQAG
                     "
     gene            complement(10684..12051)
                     /locus_tag="PKJ61_07660"
     CDS             complement(10684..12051)
                     /locus_tag="PKJ61_07660"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOC13740.1"
                     /translation="MARTTISSPAALRTDVIARYAIGSLGTGGFSTLPGLVLVFYLTD
                     TLGVAAWAAGVVVTGAKIWDVLAAPVIGGISDNSRQRTGSRRWLMTLGALALPVFFVI
                     TFAVPAGVAPAPAALWVGLAFLLASTAFSLFQVPYIALPAELSPNYDQRTRLLTARVA
                     VLSLAILLFGAGGPMLRGIGDPHTGYLVMAAVAGVVIAIGMLISATIAPVGAAKPSAP
                     LTSLTAGYREGVAMLRRSAPFRALLTAFGLQAVATGLMLAGANYVAVWVMKSNDALTF
                     LFVALIAPAILCSPLWNTVARRVGKERGFAAASVLFAVATGTLVLAVWQPGPWMYVSV
                     ALAGAAYAGMQALPMAMLPDVISHDAASSPDSSAGVFGGVWTAGETTGMALGAGVLAL
                     ILTVTGYIQTVAGTTVSQPATAVTGIALSFSIVPAALTLLSLFFLAGYRLRRTDIDEA
                     TKEDA"
     gene            complement(12175..12542)
                     /gene="ssrA"
                     /locus_tag="PKJ61_07665"
     tmRNA           complement(12175..12542)
                     /gene="ssrA"
                     /locus_tag="PKJ61_07665"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            12748..13116
                     /locus_tag="PKJ61_07670"
     CDS             12748..13116
                     /locus_tag="PKJ61_07670"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008362044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOC13741.1"
                     /translation="MEKPFVDPPEGPAPTDLEIIDLEVGTGPEAVPGKRVAVHYVGVA
                     YSTGEEFDSSYGRGEPLVFPLGARRVITGWDNGLVGMKVGGRRRLVIPPHLAYGDRGA
                     GGVIAPGETLIFVCDLVGVK"
     gene            complement(13143..14993)
                     /locus_tag="PKJ61_07675"
     CDS             complement(13143..14993)
                     /locus_tag="PKJ61_07675"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HOC13742.1"
                     /translation="MRLWRAAFASALLGVLFIALGAPAAFAASSDVFDRFDLVASVDT
                     EGYVQVTETIVLRFGSSSGRHGLERTLVTREADGDDHDVVYNIDQVSVSSPDPVSTRL
                     DLSDQGNGRNTYLRIRIGSAERTVNSATATYVLKYRVQGLLRDSGDFDELYWDLTGSS
                     MPRIVAATATITVPGGAQDVFCSVAEPGSSGPCGDGKVASSGAAQFTAANIPTGQLMT
                     VSVKITSGLVSGNTPIRVENAETAAARSTGMALLGSLAGAAVVPFIGWWYIRRRTADY
                     RFEGLPPGLFPAPGQPVTEVRNDPRMEIPVAFAPPRLAVAEAGLLVDGETQVRDTTAT
                     LVGLAVAGAIQLRSDEEQQVRLLDPSRAPDAISADLLANLFPRGSEPGTVLDLGEPGT
                     LTEAHDRAAQLALVSARNGGWFAREPGASAQLGCGLIGLMVPVAAFGIMSSAVLGPLL
                     LLSLPLVISLAATGFVVRSKLRRGQRTGAGRAWTDQVEGFHTYLATAEAEQLKFEEGE
                     DIFSKYLPWAIMFDLTERWTRVCERLVELGRLPATAPYWYYGDTWNLHHLNWQLNTLD
                     HSVGSAIASPPPSVGDTGFGSGGSAFGGGGFGGGGGFSGGGGGGGGGGSW"
     gene            complement(14990..15466)
                     /gene="smpB"
                     /locus_tag="PKJ61_07680"
     CDS             complement(14990..15466)
                     /gene="smpB"
                     /locus_tag="PKJ61_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HOC13743.1"
                     /translation="MPRPQGRIMVAQNRKARHDYHLHDQFEAGLVLTGTEVKSLRLGR
                     ASLADAFATVDDGEVWLRNAHIPEYAFGTWTNHATRRNRKLLLHRKEIAKLERETANS
                     GKTIVPLAIYFSDGYAKVEIAIATGKKDWDKRQTIAAREADREASRAMAVRNRRAR"
     gene            complement(15524..16801)
                     /locus_tag="PKJ61_07685"
     CDS             complement(15524..16801)
                     /locus_tag="PKJ61_07685"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOC13744.1"
                     /translation="MLVTSPTSRGARGSRPGPLLAFARIGVSFGLSLALTVGLTASAS
                     AKGDLDDQRDQVRKELAQTKKEIKADEVAVAEAADQLADSKSRLVKARSALAGAKQKL
                     ADAKSADAKVAAELAEAEAAAAAAAKAVVVAQAEVEAQQVLIGRVVRSAYQQQSTLVG
                     WTMVLGSETPGDLAQRLQWSTTLFDSSTSELDRLTAMEESLALAKAAKDAAEADLAAK
                     RQASAEHVAAVKKLTAEAADRADDVANLVKANAELKAAAEDDLAASEDEYRALEKEEK
                     SLSAKIRAAERKYRIVNTGGFIRPVDAPAGSPFGLRFHPILQYWRMHWGTDFGAACGA
                     PIRAMADGRVVSAGWTTYGFGNYTIISYGKVRGDYVSSGYAHQSKVVVKAGQKVKQGD
                     IVGYVGTTGLSTGCHLHLQVYRDGTRVNPMKYL"
     gene            complement(16820..17725)
                     /gene="ftsX"
                     /locus_tag="PKJ61_07690"
     CDS             complement(16820..17725)
                     /gene="ftsX"
                     /locus_tag="PKJ61_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015582567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HOC13745.1"
                     /translation="MRHTFTEAWSGLRRNASMNLAVVVTMWVSLTLFGIGLLVTQQVD
                     RLKDDWYDRVEISVFLCVPDSTSGNCEPGVGATDAQRADIEAVLRSNPEVAEVYYETK
                     AQVYEKFREYYADNDPILSATTVEAMQDSFRVKLKNPEEYQGVVSAVSGLPGVQLVQD
                     LRKILDPMFAWLNLARWATVGLSSLLLLAAALQIGNTIRMAAYSRRREIGIMRLVGAS
                     NWYIMLPFLIESLLAALAGLALAVGTLAAFQQFVVVSQATAQIKTIRWIEWSNVGTTA
                     GVLALVAVVLSIIPTLIATRRFLRV"
     gene            complement(17744..18430)
                     /gene="ftsE"
                     /locus_tag="PKJ61_07695"
     CDS             complement(17744..18430)
                     /gene="ftsE"
                     /locus_tag="PKJ61_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HOC13746.1"
                     /translation="MIRFEDVSKTYDGQSRPALNNVTVEIAKGEFAFLVGSSGSGKST
                     FLRLILREYLPTAGHIHVAGKDLNRLPSWKVPALRRQIGTVFQDFRLLPGKTVTENVA
                     FAMQVIGKPASQIRKVVPETLELVGLEGKGDRLPEELSGGEQQRVAIARAFVNRPTIL
                     IADEPTGNLDPTTSVGIMRLLDQINRADTTVIMATHDATIVDQMRKRVIELADGELVR
                     DQSKGVYGYQ"
     gene            complement(18542..19651)
                     /gene="prfB"
                     /locus_tag="PKJ61_07700"
     CDS             complement(18542..19651)
                     /gene="prfB"
                     /locus_tag="PKJ61_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015070734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HOC13747.1"
                     /translation="MTAADLQAELADLDRTLTSIESVIDPVAKRAEIAELAEQAAAPD
                     LWNDQENAQLVTSRLSRLQSEVDRIVALRERVDDLGVLIELAVEEDDPGTLAEAQKDM
                     KSLSADVEALEIRTLLSGEYDQRDALVTIRSEAGGVDAADFAAMLLRMYLRWAERHGY
                     ATEVYDTSFAEEAGIKSATFTVKEPFAYGMLSVEQGTHRLVRISPFDNQGRRQTSFAG
                     VDVLPVTEEADHIDIPEADIRVDVFRSSGPGGQSVNTTDSAVRITHLPTGLVVSCQNE
                     KSQLQNKAAALRVLQSRLLERARAEKEAEMRELKGDAGNSWGSQMRSYVLHPYQMVKD
                     LRTEHEVGNPDAVFDGDIDGFIDAGVRWRRQQGDR"
BASE COUNT         3304 a         6916 c         6564 g         3149 t
ORIGIN      
        1 gggagccgac cagcagcccg tccggcgaat cgtgcagact cgccgaacgg cagcctatga
       61 atcggtcagg cggtcgcctc ctcgacggcg tcgaggttga ggtggatcac gccgtagtcg
      121 taggccttgc ggcggtagac cgcgctgggg tggccggact cggcgtccac atagaggaag
      181 aagtcgtggc cgacgagctc catctcgtcg agcgcctgcg ccaacgtcag cggtactgca
      241 tcgaagtact tctcgcgcac aaccagcggg ccgtcgccgt ggatctccag gccggcaatc
      301 ttccggatct ccggacgggt ctcctccgcc gactccgcag gcacggccag ggcggcgtcg
      361 gccaggctcc cggtcccccg caggccacgg tgggtcttgc gccgatcgga cgcgcgccgc
      421 agctgcgact tcagccgatc gagcgcgtgg tcgaaggcca cgttcttgtc atcggcggac
      481 gcctcggcgc gtaccaccgg tccccggcta cgcagcgtga tctccacgcg cgtcgactgg
      541 tcgggctgct tcttgtagcc ataggcagaa acctgaacct caacccggat cacccggtcc
      601 ttgagcttct cgatcgacgc gatccgctcc tcaacccgag cgcggaactc atcggggatc
      661 tggcagtgcc ggccagtcac caggacgtcc atgtctacct ccttctcggt gccctacagt
      721 gcgcagggct tgcccaaaca gcaacggcac cagcccggtt ccctgccaag tggccggtga
      781 actggcgtgg gtgccatgac cccaacttag ttcgcacctg tgggctttgc cagcgcttcg
      841 gctaggaact tctcaggtgt tcgccatgcg ggcatccgac cctcaccgac agccgcgcaa
      901 cgcgaccgta actccgccta accagcccgt aacaccgcct aacctggccg taacaccgga
      961 cgcgtctgga cgtaacagca ctcggtcagc cttttgccat gattctcgaa ctcaactccg
     1021 gcgacaccgc ctggatcctc atcagtgccg cgttggtgct gctgatgacc cccggtctcg
     1081 cgctgttcta cggcggcatg gtccgagcca agggcgtgct gaacatgatg atgatgagct
     1141 tcatctcgat gggggtcatc ggcgtcctgt gggtgctgtt cggctacagc ctcgctttcg
     1201 gcaattcggt tggtggcgcc ggcatcatcg gcaacccgtt cgagttcgcc ggcctgcaga
     1261 gcctgctggt gaacgatccg gaagcgtcct accccgcact cgccttcgtc gccttccagg
     1321 cctgcttcgc gatcatcacg gtcgcgctgg tctccggcgc gatcgccgac cgtgcctcct
     1381 tcggtgcctg gactgccttc gccgtcctct ggggcacgct ggtctacttc ccggccgccc
     1441 actgggtctt cgccttctcg aaccccgacg cgggggtcgt cggcggctgg atcgccaacg
     1501 tagtgggcgc gatcgacttt gccggaggga ctgcgatcca catcaacgcc ggtactgcgg
     1561 cgctggtcct cgccctggtg attggtccgc ggatcggctt cggcaccagg ccgatgcgtc
     1621 cgcacaacat gaccctggtg atgctcggcg cagccctgct ctggttcggc tggttcggct
     1681 tcaacgccgg ctcggccgtc gcggccaacg gcctcgccag cctcgcctgg gtgaacaccc
     1741 tggccgctac cgcggcagcg atgctgggct ggctggtggt ggagaagctc cgggacggac
     1801 accccacctc gctcggcgct gcgtccggca ttgtcgcggg cctggtcgcg atcacgccgg
     1861 ccgccggtgc ggtcagtccg ctcggctcgc tcgccatcgg cgccctggcc ggtatcgctt
     1921 gcgcctacgc cgtcggcctg aagtaccggt tcggctttga cgactcactg gacgtcgtcg
     1981 gcgtccacct cgtcggcggc ctggttggca ccctgctgat cgggttcttc gccgatccgg
     2041 cgacctccgg cgatcccgcc gatcccggtg tggccggtct gttctacggc ggcgggctcg
     2101 accagctcgg caagcaggcg atcggcgcgt tcgcggtgat ggcgttctcg tttgtgatga
     2161 cctacctgat cgcgctggcg atcaagtaca ccatcggctg gcggatcagc tccgccgacg
     2221 agctgagcgg cattgacctg gccgagcact ccgaggccgg ctacgacctc agccccgtgt
     2281 actacaccaa caaggttcag cgcaccctcg tcctgaccaa ggacgacatg ccggtggccg
     2341 agacgacagg agcgaagaag tgaagttggt aacggcaatc atccagccgg agatgctgga
     2401 aaccgtccag aaggcgctgg tacggcatgg cgtgggcggg atgaccgtaa gcgaggtctc
     2461 cggctacggc cgccagcgcg gccacatcga ggtctaccgg ggtgaggagt acaccatcga
     2521 cttcatcacc aaggtgcggc tcgaagtcct ggtcgacgac gaggacgcgg aggcgctcac
     2581 cggtgtgatc gtgcaggccg cccggaccgg cgaggtcgga gacggcaagg tctggatcat
     2641 cccggtcgac accgtcgttc gagtgcggac cggcgaaagc ggctcgaccg cgatctgagg
     2701 taacgaccag ttcgaccacc cgaccagcca tcgaagtggc cactttctcg cactctcggc
     2761 gcgaaagtgg ccactttcgt tgtggacgcg cacaccagcc gagggtgttg ggtcgtgcac
     2821 ctcagggacc gctcgcgaag cttcacgaag gggtgatcct gcgcgtggtg gccgccactg
     2881 tcgcgactgc aaagggctcc cggccagcgg cccgcaacgc tcggacggcc tcgacgagcg
     2941 tcgcaccggt ggtcacgatg tcgtccacca gcaacaactc ccccggcggc agtcgccccg
     3001 cggtgaatgc gccggccagg ttggcgaggc ggccttcccg gccgagatcg gcctgatcag
     3061 cgggtcgccg cacctgcctc agccctgcgg acgcgcgcac gtccagcccg gtaccgcgca
     3121 accgccgggc cgccagccgg gcgagggcca acgtgagatc caggccacgt tcggccgccg
     3181 cggcacgcgc cgtgggcacc ggcacgatca gcaacccgcc agcggcgagc tcgggccggt
     3241 ccagcagcaa gcgggccaac gcgaccgcca gccgctcggc caggaccggc accgcaccga
     3301 gcgcgtcgcg ctccttggcc gccagcagta ggcggcgcag ttccccgtcg tagcggcctc
     3361 cggccaccac cgcaggcagt ccgggtggca acccagccac cgctaccggc cgcaccgcga
     3421 tcgccgtccg gcagccggca cagacgccca ggccggacgc gccgcagcca ggacagtggg
     3481 cgccgagcac cagctctccc ccgacgctca gcaggcgcgc aatggtcacg tccctatctt
     3541 cggcaggagt cgccagcgcg atcacccgtc cacaggctca acctgaatag gccagatcgt
     3601 ccacagcggt caccgcgagc gcccagttga actcgctctc gaagcggtag ccggcgccgc
     3661 tgtcggtcaa cgcaactgct ggccgccccg gcaccacggc gagcttgatc aaggatgctg
     3721 ccttggtggg tccaatatcc gttgcggtcg ccccgtcctc actcacccgg accacgctgg
     3781 tgtcaccggt ctccagacgc tgcagcacgg caacttccgc gggcgaccac ttcgtcgaag
     3841 tggatcggga cgcccaaccc agatcgagcg ccgcaccgtc ggtacccgtg ctcaggttca
     3901 cctccaacgg ccgccagccg gtcagccgga tctggtcatc cgtgcgcacc acgaccgcga
     3961 cccccacctc ggtgcgggtg ccgttgcgca atacgagcgc gatccgggtg ccgtccggcg
     4021 acagcttgct cgcaaccaac ggacgcttcg gcagctcact cccatccact cggatcgtca
     4081 acccatctcg gaacacccgg aagctacccg gccccgaggc cgtcgcggac cacaactcgc
     4141 cgttcctggc gaaatcgggc cgcagcagtc cggttccagt gcgcaccgtc cgcgacttgg
     4201 ccgcaccgac gggagccagg acgagcctgg tcgcggaatc cgtgatcgca gcgacttcgg
     4261 ccaggtcgct gcgcaccgcg atcagctcgg gccgggtcag gtcggcttcg accgggctga
     4321 agtccgccca attggcttcg cgcagcgcca ccacgcgccc ctcggcgatc gcgaacaggg
     4381 accgctgcgc agtcacgttg tcgggcgaca tccgggtgaa gtcgtcgccg tcgagttcgg
     4441 taccgccgaa ctccccggca atggcaaggc cgccgatact cacctgcaca gcggtcactt
     4501 gggcgaagcc cacaacggtg ttcacaatct cggccagcag gttctgtcgc tcctcggtgg
     4561 tcagccgcag cccgtccgag cccaactcga ccatggtcac cccgcgttcg tcgaggctca
     4621 cgttatccac atcgagttca ctcaccgaag cccgccgcaa cgccggcgcc agccgttcgg
     4681 acgggccgga aagcacagcc tgcaccgccg cggtcagcgc ctggtcaccg ttagcgaagt
     4741 accgctgttc gggtaccagg acgctgccgg acgcgtccag gaagtggacg gttaccgggg
     4801 tgaagttggt gctgaacagg tagcgggaga ccagcagccc gtccggcggg ttcgatatgc
     4861 gccactggtt gttggcatcg cgcaccagct cgaagtcgtg ccgcaactgc tcggaggcgg
     4921 tcgcgaagct gcccagcgag tcgagccggc cgaccagcgg ggtgatcagc atcaccgtct
     4981 ggccgaactc ctccggcggc ttcccgtccg cgtagacctg gacgccggac tcggggtgcc
     5041 agagcgcgct cgccgtctcg gtcaggtact ggcgtgcgac cgcgtaattg ggttgatgga
     5101 tgctcatcgc gtgcaggaac ccctcgacga cgagcagttg actggcaccc gtcgccgggg
     5161 gcagcgggtg gacgctcacc cccgagtccg tgccgggcac ctgttgggtg tggtgctcga
     5221 tcggcccgga cgtgggcacg gtggcgcacc cgccaagggc cagcagcacc gcgacgacca
     5281 gcgcgagcag acggttaggc atgcagcacc accttcgcgt ccggcggcac ggacggccat
     5341 ggcgaaccct gcaactcttc cgcggccttc ctgggcacgg tcagccggaa ctgggcgccc
     5401 gcgcccgggc tgccccaggc atccagacgc ccgccgtgga ggcgcgcgtc ctcgagcgag
     5461 atcgacagtc ccagaccggt gccaccgacg tgccgcccgc gggacggatc cgcacgccag
     5521 aatcggttga acacctggct cccctcctcc gcggtgaagc cgaccccgtg gtcgcggacg
     5581 gcaaccgcca cggcgttctc gtcaccgcgg acgtacaccg tgatcggcct gccctccccg
     5641 tgctcgatcg cgttcgtgat cagattgcgc agcacccggc gaatccgccg accgtccacc
     5701 tctgccacgc agttaccgcc ggagtcgagg gtgagctggg tgtgcgtccg ctcggccaac
     5761 cgctgctggg cggccatctc ctccccggcc aggacggtca ggtcgacata gtccaaggcg
     5821 agctgggcgg ctccggcatc aaagcgggag atctccagca gatcggtgag caggccctcg
     5881 aaacggtcca gttcggccga cagcagctcg gtcgaacgtc tcgacaccgc gtcgaactcg
     5941 tcccggtggg agtgcagcac ctcagcggcc atccgaatcg tggtcagcgg ggtgcgcagc
     6001 tcgtgggaca cgtcggaaac gaatcgctgc tgcaccagtg agaggttctg cagctgggtg
     6061 atcttcttgt ccagctcctc ggccatgtgg ttcatcgagc gggccaggcc ggcgaggtcc
     6121 tcggtgccga cgacggccat ccggtcctcc agatgaccgg acgcgagttg ctcggccgcg
     6181 agccgggcgg ctcgaaccgg ccgcagcacc tgcagcgcga tcaggtagac ggttagggtc
     6241 agaccggcca gcaagaagac cccggtggcg agggtggcct gttgcacgac ggccagcgtc
     6301 gccttctcct ggctggtgga gaagaggaag aagatcgggt aaggcgcctg tccgggcgcc
     6361 tgcagagtgg ctgccacgac cagtcccggc tcgtccggac gcgagtcggt gaagcggatc
     6421 agggtgggcg tgctccacaa cccgtcccca gaaccgacgc tctcccggat cgaatccggg
     6481 atgctctcgg gagccaggcc gccggtaccg atcttctgga tcggggtctc gacgaccacg
     6541 aagtactggt tgccggccag gccacggttg gtcgcctcgc gggccagccg ggtgatccgc
     6601 tcgctggcgg acgtggtgcg caggtcggtg gtggagaggt cgcgctgcat gttcgacacc
     6661 acggcggacg cttcggcgac gctcgcctgc gtcttgccgg ccatgatgcc ccgactggac
     6721 tgttccatca ggaaccagcc ggtggcggca aggatcacga tcgctgcggc gagggtggtg
     6781 accaccacct ggaatggcag cgagcgcgac cagagtcgca acgccgggtt gctggaaagc
     6841 cggctcatac cggcgttctg ggttcaccgg cgcgataacc gacaccgcgc acggtgacga
     6901 tgatctccgg cttctccggg tccttctcga tcttggaccg caggcgctgc acatgcacgt
     6961 tcaccagcct ggtgtcgctg gcgtggcggt agccccacac ctcttcgagc agcacctcac
     7021 gggtgaacgc ctgccggggc cgcctggcca gtgccagcag caggtcgaac tccagcggag
     7081 tgaggatgat ttcgttctcg ccgcggcgga ccgtgtgctc gttcacccgg atcaccaggt
     7141 cgccgatccg caggttgtcc acgtcgggtt cgggagtgat gtggcggcgc agccgggtct
     7201 tgatgcgggc gatcagctcc tgggtcttga agggcttcgc gacgtagtcg tcggcgcccg
     7261 ccgacagccc gtccaccacg tcgatggtgt cgctcttggc ggtcaacatc acgatggaga
     7321 cgccggactc ggcccggatg tcgcggcaca cgtcgacacc gtcgcgtccg gggagcatca
     7381 ggtcgagcag caccaggtcg ggacggtact cgcggaacgc ctccagcgcg gcgtcgccgg
     7441 tgtggcagag cttggcatcg aaaccctccg agccgagcac caggtgcagc atctcggcga
     7501 gggcctcgtc gtcgtcgacc acaaggatcc gcggcggcgc actccgagga gcctcagaca
     7561 ccttgtttcc ctccattggt agcagtgcac cgatactatc cgcgtcgcgg agaagttcag
     7621 ttcagttcgg tctcggcggt ccaggttgag aacagcgcca gttcaccacc ttgccggcgc
     7681 agaatacgcc gccagagcgt tgccggatcg ggatcgaaga catcggtgac cgtggcgcga
     7741 accacgtgcc acatctcgaa gtcgagttcg cgttcgagtt ggccggcgtc ccagccggcg
     7801 tagccggcga agatccgcag gtgctgatag gtgccttcgg cgatctcgac cggggtgtcg
     7861 aggttcagca gtcccacgcc gtcgaacacc ggacgccagc cggggggctc ctcgcccagc
     7921 gcccagggct cggccagaca gaccgcgccc tgttgggaga ccgggccgcc gtcgaacagc
     7981 gcgggcggct cgcagaccag ctccgcccag gctggcagtg cctccgacag ttcgatggag
     8041 gtggtccggt tgagcacgac gcccactgtg ccagtgtcat cggcgtccag cagcagcacg
     8101 acggcgccgt cgaagacccc gtcagtgatc gtgacagcgg cgaccagcag atcgccggcg
     8161 cggaacccgg tcatgccacc tcctgggttc agtctggcac cacggccaac tcgaccggaa
     8221 caaagaacga gcggcgccct tgacgggcgc cgctcgcgca gagtcgggtt ggaactacga
     8281 gtgcttgggt tccaggacgt cgagcaccgt gatcacgtgg aacggcgaac cgtcgtgctg
     8341 tgaccaatcc tcggtgaaga gatgcaggaa tcctctgatc gcctcgatcg ctacagccat
     8401 ttccagcccc cttcgcttgt caactggtga tgtggcctca gcttagcatc gctagggatg
     8461 ccacgcaagc agctctagcg atgctaagcg cgagcatcgc cgcttgtcgt gaggcatgaa
     8521 cgcccagcgc aaccccgggc cttgataccc tccagcagtg accgcttacc tgcccaggga
     8581 catttcattg gtgggacact gcgtcggtct cgccccgctg cgggaggcgg atcttccgga
     8641 tctggcgccg atcctgatgg acccggccgt ctacgcatcc ggatacgtga tgcaccgccg
     8701 gcccgcgacc accgaggatg cgctggcgct gggccgctcg aggtttctgg cagcggacgc
     8761 cccgaacggt gtcgggttcg ggcggatcgc gttcgccgtc cgactcgcga ccgattcgcc
     8821 gctggacgcg gccggttcgt tggtgggtac cacttcgctc ggcgacgcac acctggccaa
     8881 cgagagcatc cacctcggct ggacgatcta cgggtcccgc tggtggggca cggcggtgaa
     8941 cgcggagacc aagtacctgc tgctgcggca ctgcttcgag gaactcggct tcggccgggt
     9001 caagctgcag accgaccggc tgaacgttcg ctcggccgcg gcgatcgcaa agctgggcgc
     9061 gacccgggag ggcgtactgc gtcgcgagac ccgccgcgag gacggcagct tccgggacac
     9121 ggtggtgttc agcgtgctcg ccgacgaatg gccggcggtg tcagccggcc tgctggcccg
     9181 cctcaacgcc gcctgagccg ggcgttgggc ccgagcccgg ccgatcgagc ggcgacgcga
     9241 gcagttcgat cagcaaccgc tcaaccacgg acgcgggcag gatctccacc agcgccagca
     9301 ccggtgccat cgcgtgcagc gcccccggct cgaggagccc ggcgccactc aggacggcaa
     9361 aggctgcgtc ggcgtccaga ccggccagag ccgccgggtc ggcgccaccc atcagcccag
     9421 ccgcaaccgc cgtgccatcc aggtgcggtc ggccccggac ggtgtctgcg gccgccgcca
     9481 tggctgccgc caactcgtcg accacgtgcg cggtgaccgg cgtgatcgtc aggtgcgccg
     9541 tggccgggag cacggtcccg tccggctggg tcgaggccgg ctggccctgc actacgaacc
     9601 cgtgggtgcg catcgcgtcc gcccacaggt gcgggtcgac ctgcctggcc ggatcggtca
     9661 catccgcggc gagcgacagc atcgggccgg cgggcgcgcc cacgaccgac aggccgtcga
     9721 tggcaccgac cgcagcgctg agccgcgccg tggtggcagc catggacgcg gtgagctgac
     9781 ggaacccgcc aacccccagg acggacgcga tcgcccaggc agcagccagc ggaccggccg
     9841 acttcgaccc cagcagcgtc gcgttgacca ccggatagcc cggccaggaa ctcgtggcga
     9901 accattggtg gcgctgccgg tcgaacccac gctgcagcag caccgagatg cccttgggcg
     9961 cgtagccgta cttgtgcaga tcagcggaaa ggctggtgac cccgggaacc gcaaagtccc
    10021 aggcgccccc cggtgcggcg gcatcggacg gcggccagaa cgccagcgcg aacccgccga
    10081 tgcaggcgtc cacgtgcagg tcgacgccgg ccagctgtgc ggcttcggcg acctcggcga
    10141 ccgggtcgag ggcagcgaac ggatagcacg gcgcgctgac cacggccaag gcgacatcgg
    10201 ggctgaagcg ctcagccatc acaccggcgt cgacccgccc ggtggtcaca tcgaccggca
    10261 ccaggtcgag atcgagatcg aacagtgccg ctgccttgcg gaacgccgca tgcatcgtgg
    10321 ttggtgccac cagccgcgga cgctccgtgc caccgttggc gcgccaggag tcccgcgcgg
    10381 tcttgaccgc gagcaggcag ctctcggtgc cgcccgaggt cgcggtgccg gccacgtccg
    10441 gcccagcgtg gaaaacctcg cgagcgaaat cgaggagctc gcgttcgatc accgcgaccg
    10501 agctgaaagc cgtcgggtcc aggccgttca ccggctgcat cgcgcggatc gcggccgcag
    10561 cgagttcgtc cagctccggg tgcccggagt cgtagacgta ggagagcagg cgcccgccgt
    10621 gggtcggagc gtcgtgtgcg cgcaactcgg tcagccgggc gagcgtggcg gcggtgtcca
    10681 tcttcatgcg tcctccttcg tggcttcatc gatgtcggta cgtcgcaggc ggtagccggc
    10741 gaggaagaag aggctgagca gggtgagcgc ggccgggacg atgctgaagc tcagcgcgat
    10801 accggtgacc gcggtggctg gctggctaac ggtggtaccg gcaacggtct ggatgtagcc
    10861 ggtgaccgtg aggatgagcg ccagcacgcc ggcaccgagt gccatgccgg tggtctcgcc
    10921 cgccgtccag acgccgccga agacgcctgc gctggagtcc ggcgagctcg cggcgtcgtg
    10981 ggagatcacg tccggcagca tggccatcgg cagcgcctgc atgcccgcgt aggctgcacc
    11041 agcgagagcg acggagacgt acatccacgg tcccggctgc cacaccgcga gcacgagtgt
    11101 gccggtggcc acagcgaaca gcaccgatgc cgccgcgaac ccgcgctcct tgccgacgcg
    11161 acgggcgacg gtgttccaca gtggcgaaca caggatggcg ggcgcgatca gcgccacgaa
    11221 caggaacgtc agcgcgtcgt tggacttcat cacccagacc gcgacgtaat tggcaccggc
    11281 gagcatgagt ccggtggcga cagcttgcag gccgaaggcg gtcaggaggg cgcggaacgg
    11341 tgcgctgcgc cgaagcattg cgacgccttc gcggtagccg gcggtcaggg acgtgagtgg
    11401 ggcggacggt ttggcggccc caaccggtgc gatggtggcc gagatgagca tgccgatcgc
    11461 gatcacgacc ccggcaaccg ctgccatcac caggtagccg gtgtggggat cgccgattcc
    11521 ccgcagcatc ggaccgcccg cgccgaacag gaggatggcg aggctgagca ccgccacgcg
    11581 ggcggtgagc agccgcgtcc gctggtcgta gttgggcgag agttcggcgg gcagagcgat
    11641 gtagggcacc tggaagaggc tgaacgcagt ggacgcgagc aggaaggcca ggcccaccca
    11701 gagcgcggcc ggtgccggcg caaccccggc cgggacggca aaggtgatga cgaagaacac
    11761 cggcagggcg agggcgccga gggtcatcaa ccagcggcgg gaacccgtcc gctgtcgtga
    11821 gttgtcggag ataccgccga tcaccggggc ggcgagcacg tcccagatct tcgccccggt
    11881 gacgacgacc cccgcagccc acgcggccac cccgagcgtg tcggtgaggt agaagaccag
    11941 caccagccca gggagcgtcg aaaatccgcc ggtgccgagc gagccgatgg catagcgagc
    12001 gatcacgtca gtgcgcaacg ccgcgggcga ggaaatcgtc gttcgggcca cggccggagc
    12061 ctacgacccc gaacaccacc cgcacagtgg tccacgagac ttcgctcgac ctagggccag
    12121 gcgagcagag ggccccgtgg ccgctttcgc ggaccacggg gcctctgttc acaggggtgg
    12181 aggtggcggg agtcgaaccc gcgtcctcaa gcggcgaacc agacattctc cgggcgcagc
    12241 tggctagtcg ttctacttgg ctctcaccct cgcgccagca cgtggtgaac aagcccagtt
    12301 ccatgaatgt cctgcactga ccctggaacc aagtcagttc agtaagcctt ctagatgagg
    12361 ccagttaccg gatcgaaggc atatccgggc tgacccttcg gtcgctgatc aggcagcgag
    12421 agcgaagtca gtgcgcgttg agttggcact tattggtttc cgagattcgt taacgagatg
    12481 tcctcggatc ctcggcccgc ttctcctgga actaccgtcc caagtcgaaa ccagtcaccc
    12541 cctgttgagt tgtaccgagc tctgggagct cagctttcag agtatacgcc gtcgcccgcg
    12601 ccgaggcgac ttacaggcac ggcgacgccg aaaccagcac cccggccacc gtcggggatg
    12661 tgtcatcgaa cccggggggt cagccgaacc agccagccga accggggcaa cccgaggaca
    12721 gcgggccaac cgggtagggt gccggtcatg gagaagccat tcgtcgaccc gcccgagggt
    12781 cctgcaccca ccgatctgga gatcatcgac cttgaggtcg ggaccggtcc cgaggccgtg
    12841 cccggcaaac gcgttgccgt ccactacgtc ggcgtcgcgt actccaccgg tgaggagttc
    12901 gactccagct acggccgtgg cgagcctctg gtcttcccgc tgggtgcccg tcgggtcatc
    12961 accggctggg acaatggact ggtcggcatg aaggtcggcg gacgccgccg tctggtcatc
    13021 ccaccccacc tcgcctacgg cgaccgcggc gccggcggcg tcatcgcgcc gggcgagacc
    13081 ctgatcttcg tgtgcgacct ggttggcgtg aagtagccga gccagatcgg ctggcggcgg
    13141 ggctaccagc ttccgccgcc accaccgcca ccaccgccgc cggagaaccc accaccgccg
    13201 ccgaagccgc cgccaccgaa ggcggaaccg ccactaccga agccggtgtc ccccaccgaa
    13261 ggcggcggcg aagcgatcgc actgcccacc gagtgatcga gcgtgttcag ctgccagttg
    13321 aggtggtgca ggttccaggt gtcgccgtag taccagtagg gcgccgtcgc aggcagccgg
    13381 cccagttcga ccaaccgctc gcacacccgc gtccatcgtt cggtcaggtc gaacatgatc
    13441 gcccagggca ggtacttcga gaagatgtcc tcgccctcct cgaacttaag ctgttcggcc
    13501 tcggccgtag ccagataggt gtggaagccc tccacctgat cagtccaggc acgtccggca
    13561 ccggtccgct gcccccgacg caacttcgag cgaaccacga agccggtggc cgccagcgag
    13621 atcaccagcg gcagcgacag cagtagcaac ggtccgagca cggcactgga catgatgccg
    13681 aaggcagcca cgggcaccat cagcccgatc aagccacagc ccagctgcgc actagcaccg
    13741 ggctcccgcg cgaaccagcc accatttcgt gcgctcacca gtgccaattg cgcggcccgg
    13801 tcgtgtgcct cggtcaaggt gcccggctcg cccagatcga gcaccgtgcc gggctccgaa
    13861 ccgcgtggga acaggttcgc gagcaggtct gcgctgatcg cgtccggggc ccgggacggg
    13921 tccagcagcc gcacctgctg ctcctcgtcg ctgcgcaact ggatcgcgcc ggccaccgcg
    13981 agcccgacca gcgtggcggt cgtgtcccgt acctgggttt cgccgtcgac cagcagtccg
    14041 gcctcggcga ccgcgagccg cggcggcgcg aaggccaccg ggatctccat ccgcgggtcg
    14101 ttgcgcacct cggtcaccgg ctgacccggc gcggggaaga gccccggcgg caagccttcg
    14161 aaccggtagt cggccgtccg ccggcggatg taccaccaac cgatgaacgg caccaccgcg
    14221 gctcccgcca gcgaacccag cagcgccatg cccgtcgatc gggcggccgc cgtttcggca
    14281 ttctccaccc ggatcggagt gttaccgctg accagccccg aggtgatctt caccgatacg
    14341 gtcatcagct ggccggtagg gatgttggct gccgtgaact gcgcagcccc cgacgacgcc
    14401 accttgccat ccccacatgg ccccgacgag cctggttcgg cgaccgagca gaacacgtcc
    14461 tgcgcgccgc cgggaactgt gattgtcgcc gtagccgcaa cgatccgcgg catcgacgag
    14521 ccggtgagat cccagtacaa ctcgtcgaag tcgcccgagt cccgcaacaa gccctggacg
    14581 cggtacttca gcacataggt cgccgtcgcc gagttcaccg tccgctcggc cgaaccgatc
    14641 cgaatccgca ggtacgtgtt gcgaccgttc ccctggtcgc tcaggtccaa ccgcgtcgat
    14701 accgggtcgg gactggagac cgagacctgg tcgatgttgt agaccacgtc atggtcgtcg
    14761 ccgtcggctt cgcgggtgac cagcgtccgc tccaggccgt gccgtccgga cgacgacccg
    14821 aaccgcagca cgatcgtctc ggtgacctgc acgtagcctt cggtgtccac gctcgccacc
    14881 aggtcgaagc gatcgaacac gtctgatgac gccgcgaacg cggccggcgc tccgagcgca
    14941 atgaacagca ctcccaacag ggcggacgcg aaggccgccc gccacagcct catcgtgcgc
    15001 gccggttgcg gacggccatc gcccggctcg cctcccgatc agcctcgcgc gcggcgatgg
    15061 tctgccgctt gtcccagtcc ttcttgccgg tggcgatggc gatctcaacc ttggcgtagc
    15121 cgtcggagaa gtagatcgcc aacggcacga tggtcttgcc ggagttcgca gtctctcgct
    15181 ccagcttggc gatctccttg cggtgcagca gcagcttgcg attgcgccgg gtggcatggt
    15241 tcgtccaggt gccgaaggcg tactccggaa tgtgcgcgtt ccgcaaccag acttcgccgt
    15301 cgtcgacggt cgcgaacgcg tccgccaatg aagcccgccc cagccgcagc gacttcacct
    15361 cggtgccggt cagcaccaac ccggcctcga actggtcgtg caggtggtag tcatggcggg
    15421 ctttgcggtt ctgggcaacc atgattcggc cctgcggtct cggcatggtc tctccctccc
    15481 tggaacgctt ggcagcctac caaggcgtac cgacaacgct gcgtcagaga tacttcatcg
    15541 ggttgacccg cgtaccgtca cggtagacct gcaggtgcag atggcagcca gtgctcaggc
    15601 cggtcgtgcc cacgtagccg acgatgtcgc cctgcttgac cttctggccg gctttcacca
    15661 ccaccttgga ctggtgcgcg tagccgctgg agacgtagtc gcccctgact ttgccgtagc
    15721 tgatgatcgt gtagttgccg aagccatagg tggtccaacc ggcggagacc acccggccgt
    15781 cagccatcgc gcggatcggt gccccacatg cggcgccgaa atcagtaccc cagtgcatcc
    15841 gccagtactg caggatcggg tggaagcgca gcccgaacgg agagcccgcg ggtgcatcca
    15901 ccggacggat gaagccgccg gtgttaacga tccggtactt ccgctcggcc gccctgatct
    15961 tggccgagag gctcttctcc tccttctcca gcgcgcggta ctcgtcctcg ctggccgcaa
    16021 ggtcatcctc ggccgcggcc ttcaactcgg cgttggcctt taccaggttg gccacgtcgt
    16081 cggcccggtc ggccgcctcg gcggtgagct tcttcaccgc agccacgtgc tccgcggacg
    16141 cctgccgctt ggccgccaga tcggcttccg ccgcatcctt ggcagccttg gccagtgcga
    16201 gtgactcctc catcgcggtg aggcggtcga gttcgctggt ggaactgtcg aacagcgtcg
    16261 tcgaccactg cagccgctgc gccaagtcgc ccggtgtctc cgagccgagc accatggtcc
    16321 acccaaccag ggtggattgc tgctggtagg ccgaccgcac gacccgtccg atcaggacct
    16381 gctgcgcctc gacctcggcc tgggctacga cgacagcctt tgccgcagcc gctgccgcag
    16441 cttccgcttc ggccagttcc gcggcgacct tcgcgtccgc ggacttggcg tccgccagct
    16501 tctgcttggc accggccagc gcggaacggg ctttcaccaa tcggctcttg gaatcggcca
    16561 actggtcggc ggcttcggca accgcaacct cgtcagcctt gatctccttc ttggtctgcg
    16621 caagttcctt gcgcacctgg tcgcgctggt cgtcaaggtc gcccttggcc gaagcggacg
    16681 cggtcaggcc gacggtcagc gcgagcgaga gcccgaacga gacgccgatg cgcgcgaacg
    16741 ccaacagcgg gccggggcgg ctaccccggg cgccgcgtga ggtgggggaa gtcaccaaca
    16801 cgagagcccc aatcaaatat cagacccgca ggaaccgccg ggtcgcgatc aaagtcggaa
    16861 taatcgaaag cactactgca actaatgcca gaacaccggc cgtggtgcca acattcgacc
    16921 actcaatcca gcgaatagtc ttgatctgcg ctgtagcctg gctgaccacc acgaattgct
    16981 ggaaagccgc caaagtgccg accgcgagcg ccagaccggc gagcgcagcg agcaacgact
    17041 cgatcaggaa cggcagcatg atgtaccagt tcgaggcgcc gaccagccgc atgatgccga
    17101 tctcgcgccg ccgcgaatag gcggccatcc ggatcgtgtt gccgatctgc agggctgcgg
    17161 ccagcagcaa cagcgaactc agccccaccg tcgcccagcg ggcgaggttc aaccaggcga
    17221 acatcgggtc gaggatcttg cgcaggtcct gcaccaactg gaccccgggc aggccgctca
    17281 ccgccgaaac cacgccctgg tactcctccg ggttcttcag cttcacccgg aacgaatcct
    17341 gcatcgcctc gaccgtggtc gcgctgagaa tcggatcgtt atcggcgtag tactcgcgga
    17401 acttctcata gacctgggcc ttggtctcgt aatagacctc ggcgacctcg ggattactcc
    17461 gcaataccgc ctcgatatcg gcgcgctggg catcagtggc gcccacgccc ggctcgcaat
    17521 tcccactggt ggaatccggt acgcacagga acacgctgat ctcgacccgg tcgtaccagt
    17581 cgtccttcag ccggtcgacc tgctgggtga ccagcaggcc gatgccgaac agggtcagcg
    17641 agacccacat ggtcaccacc acggcgaggt tcatggacgc gttgcgccgc agcccggacc
    17701 aggcctcggt gaacgtgtgc cgcattaggc ggtcctcccc aactcactgg tagccataca
    17761 cgcccttgct ctggtcacgc accagctcac cgtcggcgag ttcgatcacg cgcttgcgca
    17821 tctggtcgac gatggtggcg tcgtgcgtcg ccatgatcac ggtggtgtcg gcacggttga
    17881 tctggtccag aagccgcatg atgcccacgc tggtggtggg gtcgaggttg ccggtcggct
    17941 cgtccgcgat caggatcgtc ggacggttga cgaaggcccg ggcgatggcg acccgctgct
    18001 gctcgccgcc ggagagctcc tcaggcaggc ggtcgccctt gccttcgaga ccgaccagtt
    18061 cgagggtctc cggcaccacc ttgcgaatct gggacgccgg cttaccgatc acctgcattg
    18121 cgaacgccac gttctcggtg acggtcttgc cgggtagcag ccggaaatcc tggaacacag
    18181 tgccgatctg ccggcgcagc gcaggtacct tccagctggg cagtcggttg aggtccttac
    18241 cagccacatg gatgtgcccg gcggttggca ggtactcgcg caggatcagg cgaaggaacg
    18301 tcgacttacc cgaaccggac gagcccacca ggaaagcgaa ttcacccttg gcgatctcga
    18361 cggtgacgtt gttcagcgca ggccgagact gaccgtcgta ggtcttggag acatcctcga
    18421 atctgatcac gattgcgtcc gaccggttag cgaatgggga agtttccttg acaggggcca
    18481 aggccggttc cgtcggagtg tatgcgacac cgtgacatga cccaagcctc gccccggcgt
    18541 gtcagcgatc accctgctgt cgacgccagc gcaccccggc atcgatgaat ccgtcgatgt
    18601 caccatcgaa gaccgcgtcc gggttgccga cttcatgctc ggtgcgtaga tccttgacca
    18661 tctggtacgg gtgcagaacg taggaacgca tctgcgagcc ccacgagtta ccggcatcgc
    18721 ccttcagctc gcgcatctcg gcttctttct ccgcacgggc ccgctcaagc agccgcgact
    18781 gcaggacgcg aagtgcggcg gccttgttct gcagctgcga cttctcgttc tgacaggaca
    18841 ccaccagccc ggtgggcagg tgcgtgatcc ggacggcgga gtcggtggtg ttcacgctct
    18901 gcccgccggg accggacgaa cggaagacgt ccacccggat gtcggcctcg ggaatgtcga
    18961 tgtggtcagc ctcctcggtg accggaagca cgtcgacacc ggcgaaggac gtctgccggc
    19021 gtccctggtt gtcgaagggg ctgatccgga ccaggcggtg ggtgccctgc tcgaccgaca
    19081 gcatgccgta cgcgaacggc tctttgaccg tgaacgtagc cgacttgatc ccggcctcct
    19141 cagcgaagga ggtgtcgtag acctcggtcg cgtagccgtg ccgctccgcc cagcgcaggt
    19201 acatgcgcag gagcatggcc gcgaagtcgg ccgcgtccac gccgcccgct tccgagcgaa
    19261 tggtcaccag cgcgtcccgc tggtcgtact cgcccgagag cagcgtgcgg atctccagcg
    19321 cctcgacgtc tgcggagagc gacttcatgt ccttctgcgc ctcggccagc gtgccggggt
    19381 cgtcctcctc gacggcgagt tcgatcagca cgccgagatc gtccacgcgc tcgcgaaggg
    19441 cgacgatccg gtccacttcg gattggagcc gggatagccg agacgtgacc agctgggcgt
    19501 tctcctggtc gttccagagg tcgggcgcgg cggcctgttc ggccaactcg gcgatttcgg
    19561 cgcgcttggc gaccggatcg atgacgctct cgatggaggt cagcgtccgg tccagatcgg
    19621 ccagttcggc ctgcaggtca gcggcagtca cagcggtcca ctctaccttg tcgacgcctg
    19681 cctcccagcg ccggtgaggc gccgaaacga cgccgatctt gaggcagggc aaacttcaac
    19741 gacgaacctt gactgcactc ggcgtttcgc tcgtaatcgg catcaggtgc gctagcatcc
    19801 tgtgcgtcgg tgatgagtcc cccgagtgat caccacccgc ttggttaccc accaggcgag
    19861 ctgcccccgg ccgtgcccgc agagcgaaag ctcgtggcgg gcaactcgac tttctgggcc
    19921 cgaatcgtgg gca
//
LOCUS       DAOPRQ010000049        19899 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_2308_length_19899_cov_11.619532, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000049 DAOPRQ010000000
VERSION     DAOPRQ010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 19899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19899
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2308_length_19899_cov_11.619532"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..845
                     /locus_tag="PKJ61_07705"
     CDS             <1..845
                     /locus_tag="PKJ61_07705"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002545536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HOC13748.1"
                     /translation="VPFGIGQVGKSFRNEITPGNFIFRTREFEQMEMEFFVKPGTDEE
                     WHQHWIDARTSWYTDLGISPENLRHYEHPKEKLSHYSKRTVDIEYNFGFTGGDGWGEL
                     EGIANRTDFDLGTHSSHSGTDLSYFEQASGERYLPYVIEPAAGLTRSLMAFLVEAYTE
                     DEAPNTKGGVDVRTVLKLDPRLSPVKAAVLPLSRNADLSPKARDLAAQLRKVWNVEFD
                     DAQAIGRRYRRQDEIGTPFCITVDFDTLTDDAVTIRERDTMSQERVALDQVQSYLGAR
                     LIGC"
     gene            complement(853..1428)
                     /locus_tag="PKJ61_07710"
     CDS             complement(853..1428)
                     /locus_tag="PKJ61_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017932723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HOC13749.1"
                     /translation="MSIVHGQVTSALALVPRVEFLPPDQRTRAAADEPLPIGHGATNS
                     QPWTVQFMLELLHVPEGARVLDVGSGSGWTTALLAQLAGPRGSVLGLEVVPELVEFGR
                     ANLARFDLGWARIEPAGKGTLGAPEAGPFDRILVSADAGGVPSALEEQLAIGGRMVLP
                     AAGVMWVIDRDHEGWHREPVMGYRFSFVPLV"
     gene            1643..2788
                     /gene="dusB"
                     /locus_tag="PKJ61_07715"
     CDS             1643..2788
                     /gene="dusB"
                     /locus_tag="PKJ61_07715"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002517927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HOC13750.1"
                     /translation="MAETLRLAAPSGGTVEVSTPVVLAPMAGITNAAYRQLCREQGAG
                     LYVCEMITSRGIVERIPKTFDMLRFDAGETVRSVQLYGVDPPVLAKATEILCAEFGVS
                     HVDLNFGCPVPKVTRRGGGGVLPWKRDRLAAILRATVRAADRYGVPVTMKTRLGIDAD
                     HLTYLDAGRIAQDAGCAAIALHARTVQQAYSGEAAWETIHTLVEAVDLPVLGNGDIWE
                     ASDALAMVAQTGCAGVVIGRGCLGRPWLFRDLADAFAGRDRRTLPSLGEVAAMVRRHA
                     ELLAELYGEKHGLTDLRKHMAWYFKGFPVGGEIRRGLAMVSSLAELDDRLGQLDPSVP
                     FPEAELGAPRGRQGTPRDKVTMPYGWLDSCELGDADIADAESDASGG"
     gene            complement(2952..3644)
                     /locus_tag="PKJ61_07720"
     CDS             complement(2952..3644)
                     /locus_tag="PKJ61_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13751.1"
                     /translation="MSQTEGTQPPNPGPGDQYSQTGQPAPTPYAPGQYPPAQYPSAQY
                     PSAQYPPVQYPQGAYPQSQPAPGPYTGPYAQTGQPAQQYPSAYGQQPYGAYGQPYPTT
                     SYPGAPVKAKKSPVLGIVALAVVAMSLIVSIFALQPVMAVMASVIAQSGLETVDQDYL
                     AEVLVANAAGPAMILNIVSLVGFAGFVTGIVAIVVNRGRLWGVLATVLGILAPIVLLA
                     VMLMALMPYAGA"
     gene            3735..4841
                     /locus_tag="PKJ61_07725"
     CDS             3735..4841
                     /locus_tag="PKJ61_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOC13752.1"
                     /translation="MPELQPRAVAWAETMAGHPGERFVRAHATSGPSLELREPVLREQ
                     REAWEAVHLTPGSAYALGAGERARSEEPDAERTCFERDRDRIVHSTAFRRLAGKTQVV
                     VFPTDHQRTRLTHALEVAQVATSIARGVGANGSLAEAIALGHDCGHGPGGHASEDAFD
                     AFLPEGYDHGPWGADVVLAGLNLCAQTLDGIRNHSWSRPVPATVEGEIVSWADRIAYC
                     AHDLEDAAGAGIVDLVDLPAEIAGVCGRTRRQQLGTFVRAVIETVASTGQVGMRPDEA
                     GALAGLRAFNYERIYTRPDSVAQSRAVIDVLHALVEYYAKHPSAMPADFVDGNVRGAV
                     TYVAGMTDRFAFETAAELLGWDADRLPKGIGRGA"
     gene            5023..5409
                     /locus_tag="PKJ61_07730"
     CDS             5023..5409
                     /locus_tag="PKJ61_07730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13753.1"
                     /translation="MAERTGPWPVLGGAAIGLVLAGVLAMLNTPATAIAAVLLFGLAA
                     APIYPLLTLTTAERTSAAIVDTVVGLRAGASSLGPAILPLVVGLAMNQSISAVAPVLL
                     VACLIVAALHAVLQLRRAVGRGPQIG"
     gene            5444..7327
                     /gene="dnaG"
                     /locus_tag="PKJ61_07735"
     CDS             5444..7327
                     /gene="dnaG"
                     /locus_tag="PKJ61_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOC13754.1"
                     /translation="MAGLINEEDLATVRERSRIEDVVASFVTLRRAGGGSLAGLCPFH
                     DEKTPSFRVTPARGFWHCFGCGEGGDVISFVQKINNVGFAEAVEYLADRVGVQLRFSD
                     AGGARVEPGLRMRVLEANRLAAEFYTGHLLSATGLPGRQFLDGRGFDKTAAEQFGIGF
                     APTGGRELGQHLKAQGFSDEELVRAGLVRENGWDFFQGRVLWPIRDAGRSVLGFGARR
                     IFDDDRMPAKYLNTPETIVYKKSNVLYGLDLARGNIAKKNQAVIVEGYTDVMACHLAG
                     VDTAVASCGTAFGDEHSRLLQRLLGTGDVSGGEVIFTFDGDAAGQAAALKVFKGDQNF
                     SAQTYVAVEPSGLDPCDLRIQQGEAAVRELVGRRVPLYQFVMRNILAEHDLDRVDGRL
                     AALRAAAPLVSSIRDKSLFERYIRELAKWLGDPDPNEVRREVNATMHRRPAEQRRQAD
                     RRPPEVADAALPGLPEPAPSLPVPDPRDRRLEAERGTLRLLVRAPEKFVDDWFGLVPG
                     DFTHPTYRALFEVVQGVADGAGPWTQRMLAANPDPVLESLVISLAVEPVLREPTEAYA
                     REYAAQLRVQTVSRQIAELKSKLQRTNPLADQTAYNRMFAQLVNLEKDRKDLVALATG
                     PAL"
     gene            7422..8285
                     /locus_tag="PKJ61_07740"
     CDS             7422..8285
                     /locus_tag="PKJ61_07740"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOC13755.1"
                     /translation="MRSLRSLHLPTTDEEVAWARAIEAGVLAARLLVTGERPVPATDA
                     ELSALVEAGHRARQRFLLGYVRLVAKLAAAEAKRCGLNPEDLCQEGFVALCDTLQRFD
                     HTRCRFSTYATMRVGQHLAEVGAARLGDLAIPASRALRLRRARGVAAALGQEQQRTVG
                     AAEVAAAVGASARDMSRLLAYRAPVPVDVLAEAIPDPDPVDPDAGIYAEQFGRLVARL
                     EPTQAEIVRLRYGLVTGEQLDVQQTARLLGLSESSVRRLERKALAALRPMLEATPAGR
                     YYREPPARWTA"
     gene            complement(8233..9231)
                     /locus_tag="PKJ61_07745"
     CDS             complement(8233..9231)
                     /locus_tag="PKJ61_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008356895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOC13756.1"
                     /translation="MELSRNVTITDVARSAGVSVATVSKVINDRYGVAKLTERRVRQV
                     IAELGYESSLVAKSLRAHRTGVLGILVAEFEPFSTEVLKGLASGAKDSGYELLAHSGG
                     TGPNGHEGWEMRSLSRLSGTLIDGAVLVTPTVVDGHPRIPVVAIDPHTGTTVLPTIDC
                     DNLGGADSAVEHLLGLGHRRIGFLAGRPDLQSALLREEGYRRTLAAAGLDVDENLIRV
                     GGYRAETAGGPALELLSLPQRPTAVFAANDLTAIRTIEVAESLGLRVPEDLSVVGFDN
                     VPDAAQANLTTVAQPLHEIGERAMRTLLALLAGEAAADHVTLSTELVVRGSTAPPA"
     gene            9354..10676
                     /locus_tag="PKJ61_07750"
     CDS             9354..10676
                     /locus_tag="PKJ61_07750"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOC13757.1"
                     /translation="MKALRKRSAAAVAMIGAVSLLAACSTGGGTATQPAATGDTTASA
                     EAVTMSFWHNSTTGDGKKYWEDTVAAFTKEHPNVTINIQAVQNEDMDGKLQTALNSGD
                     DPTIFMARGGGKLADVVAADQVLDITDKIDPAIKSALGDGIFSAFTVDGKIYGMPTSV
                     LPGGIYYSKDLFAKAGITAVPTTFDELNAAVEKLKAIDVAPIALGGKDAWPAAHWYYF
                     MALRNCSKDVMDKAAAEKDFSDPCWLKAGEDLKAFTATDPFNKGFLTTSAQQGAGSSA
                     GMLANHKAAMELMGAWNPGVIADLTPDKKALPDLGWFPFPAVNGGAGDASAMMGGSDG
                     FACRKNAPAACVDFLNFMATKANQEGYATAFKTLPANKEAKGVVTDPALQDVLKSYDS
                     AAYVMLWLDTMYGQNVGNALNGGVVNMFAGKGEPTDIVEAVKSAAAKG"
     gene            10697..11707
                     /locus_tag="PKJ61_07755"
     CDS             10697..11707
                     /locus_tag="PKJ61_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017935239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOC13758.1"
                     /translation="MSDSVTALPKRSSRSGSERGAATAATRSDRARAEVRKRFEIAVL
                     AGPAIVMFLAFVIFPVFMAAYYGFFKWKGFGAPTNFVGFANYVVIFSDQAFRDALVHN
                     GFILVMSLVMQGPAAIAFALLLNQNIRGRSLIRVLIFVPYVISEVIVGTGWSLMLQYT
                     GAVNDLLTNIGLGALAHDWIADPSVAIWTLMVIISWKYIGFAVILMLAGIQGIPDELY
                     EAAAIDGASYWQIQRWITLPLLGPTIRVWAFLSIIGSLQLFDLVYIIWGQYVASTAGT
                     STMATYMVREGRLAGNYGYGNAVAVVIFIISLLIALTYQRFVMRRDTEGALTGASGRK
                     RK"
     gene            11707..12588
                     /locus_tag="PKJ61_07760"
     CDS             11707..12588
                     /locus_tag="PKJ61_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017935240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOC13759.1"
                     /translation="MSQTSLTARTGPSRRIATSPSGATWGSPTTYLVATVLIAVCIAP
                     VIYIILGGFRSNSQITVNPSGWPDPWVLTNYTAVLTSSLFWQQFTNSVISAVSTTVGT
                     VALALMASFVIARYSFAGRNAMYALFAAGLMFPLTVAITPLYLLIKNLGLTDNLLGII
                     LPQIAFALPMTVIILVPFLAAIPKELEEAAAIDGASRLKFFVRMVLPLSLPGVITTGI
                     LTFVNAWNSYMLPLFILNDSKMYTLPLGVQAFSSEHSVDTAAVLAFTSLSMLPALLFF
                     SVFERRIVGGLTGAVKG"
     gene            12637..>13788
                     /locus_tag="PKJ61_07765"
     CDS             12637..>13788
                     /locus_tag="PKJ61_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017935241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HOC13760.1"
                     /translation="MSTIVAGLPAFPAVSARVRDLVDGMTLPEKLAQIVGYWLDQGGN
                     VVAPMQGELGKAGATGELAAVTADGIGHYTRVYGTRPVEPAARAAWLWDEQRRLQRET
                     RLGIPALVHEECLTGLAAWQATTFPTPLAWGASFDPELVQQAAAAIGESMRSLGVHQG
                     LAPVLDVIRDPRWGRVDECIGEDPYLVGTVGTAYVRGLQSAGVHATLKHFLGYSASIA
                     GRNHAPVHVGPRELADVFLPPFEMAILDGAAASVMNSYTDIDGIPVAANPDLLTEVLR
                     ERLGFTGVVVSDYFAVAFLQVMHAVAADRGEAAALALIAGIDVELPSGDAYLEPLAEM
                     VRTGALDEAYVDRAVLRTLAQKEALGLLEPDAFAEDAPAAIDLDPPEHQA"
     assembly_gap    13789..13888
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <13889..14895
                     /locus_tag="PKJ61_07770"
     CDS             <13889..14895
                     /locus_tag="PKJ61_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008356888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HOC13761.1"
                     /translation="VPGCAVEGADTSGIAAAVATATDADVAIVVVGDQAGLFGRGTVG
                     EGNDTDSLDLPGVQRELVEAVVATGTPVVLVLLTGRPYAIGWALDGPGPRPAAVVQAF
                     FPGEGGGLAIADVLTGAVNPSGRLPVSLPRSSGAQPYSYLQPILGGPSEVTATDPTPL
                     RPFGFGLTYTTFDYTDLVVDPAVAAGGNFTAAVTVTNTGRVVGAEVVQLYGRDVRASV
                     VRPVVQLLGYARVELAAGESRRVTFDVPTTRFAFSDRRLVRVVEPGEVEVWVASHAAA
                     SELVQSVGDTNGSIPSSDRRASAVPGTATSRASLAFTGEVHEVTVADRRVVGVRVDVA
                     "
     gene            14892..16451
                     /locus_tag="PKJ61_07775"
     CDS             14892..16451
                     /locus_tag="PKJ61_07775"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HOC13762.1"
                     /translation="MTRVDNPILPGCHPDPSICRVDGTYYLVTSTFEYFPGLPIFAST
                     DLAHWELIGHVVTRPEQLDYAGIASSGGLYAPTLRHHDGRYWVVCTLVDQQDASRGGN
                     FLMTATNPAGPWSDPVWLDAAGIDPSVFFDDDGRVWLHGTRLAAEPEWHDQTEVWVRE
                     FDPVTMALVGPETVIWTGAVRGAVWAEGPHLYRVDGTYYLLAAEGGTEFHHAVSVARA
                     DAVTGPFVGNPANPVFTHRHLGREVDVVGAGHADLVQAPDGSWWAVLLAMRPYGGYHY
                     NLGRETFLVPVTWQDGWPVFAPGVGRIPAQVELPTAAAVPLSPEQGGASGVVLPDDPR
                     WTSLRGPASAFAEPDGDGWRLRLRPTTLAEPGTPAFLGVRQQHRNLNLRVTLHAPLSA
                     GEEAGIVVRQSELDHVRLFLRPDTDRHGIVQAVHRRGGVETVLGEWATAAPVGDRLVL
                     TVEIRNQAYALVAQLDAGPKTRLGVVDGSQLDSVATGGFLGLWLGVYGTSNGAESTTT
                     VAVDSVEYLPD"
     gene            16462..16851
                     /locus_tag="PKJ61_07780"
     CDS             16462..16851
                     /locus_tag="PKJ61_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13763.1"
                     /translation="MERILGLLLVLTLSGCSLLGSSSWDQVKVAFSGADKGDYTLVVT
                     PKQAIYTIDGKATSEDLPEGAWTALATGVAALGARTSKECDGQRLKIQALSGGDVLQT
                     FEATSCDGGEALEQAKGVLDQLIAQLR"
     gene            16862..17833
                     /locus_tag="PKJ61_07785"
     CDS             16862..17833
                     /locus_tag="PKJ61_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HOC13764.1"
                     /translation="MGIPLIIDTDTAQDDCVTILLALLDPAADLRAITMVAGNVGFEQ
                     QVANAHLTLNVAGKLGQVPVHLGCRQPMLRQWESAEDVHGDGSGGLSMDLAAVTTEPE
                     HAVDALLRLTSESPGELSIIAIGPLTNLATAVVMDPGFAKRVKSLYIMGGSNNGRGNI
                     TASAEFNFYVDPEAAQIVLDAGFRDLLILPWDPVTLRDATIDRAGYDRLCALGTPLAR
                     FFRRMCDATIDYDESVGVNGSTHPDSLTLAVLLHPELILAEAPYRVDVETRSELTRGY
                     SAMAWEKFDLVPNARVVEAIDTAAFFGQLADLLRTETVPDLPIAGID"
     gene            complement(17830..18672)
                     /locus_tag="PKJ61_07790"
     CDS             complement(17830..18672)
                     /locus_tag="PKJ61_07790"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HOC13765.1"
                     /translation="MSVLPDPILPEHPIDVRLVAADLDGTLLDAAGAVPAELAGVLHR
                     LRERGIVFAPASGRPYASLLHAFASVADEIDAYIADNGTYVVHHGVDVITSYLAPETV
                     AAVVRGVRELKASGRVDLGLVVSGKELSYAERADRRFTEAAGHYYANLTATDDLLAHT
                     TNALKLSIYVFGDPHEVADKIAGLCPDAQVVVATTHWVDVMSVGVNKGVALARLQEAL
                     GVTRAQTVAFGDYLNDLEMLAQAEHSFAMANSHPDVFDTAAYIAPSNVAMGVVATLNH
                     LLDL"
     gene            complement(18870..19184)
                     /locus_tag="PKJ61_07795"
     CDS             complement(18870..19184)
                     /locus_tag="PKJ61_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13766.1"
                     /translation="MAEMDAGKDPVDLAIDTKHHRLFVASSGDDESLLAYDTRTNKRV
                     GTYPSGHFAALATDPAAGLLYALDEWGQVALLRSTTLKRQDTLALRPGRELTIDTKRQ
                     RL"
     gene            complement(19263..19601)
                     /locus_tag="PKJ61_07800"
     CDS             complement(19263..19601)
                     /locus_tag="PKJ61_07800"
                     /inference="COORDINATES: protein motif:HMM:NF013561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HOC13767.1"
                     /translation="MTEQQRPERFRSGFGPDVTIRDIDLDTEEFVVGGEPLTEARAKE
                     IADRLERRGGRPSLTAPGAHSPVLNLRVSQVTKEQLEHLAEESGRRQSDLVREALTEY
                     LARHGESRAS"
     gene            complement(19598..19831)
                     /locus_tag="PKJ61_07805"
     CDS             complement(19598..19831)
                     /locus_tag="PKJ61_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13768.1"
                     /translation="MGLAQIRITSTARRRKLSSSRIREALTNAVFDHMDEDVAIYVGT
                     DGRGLVIELGIVADDRGEGFAVLHAMPRERRNR"
BASE COUNT         3012 a         6687 c         6854 g         3246 t
ORIGIN      
        1 aggtgccgtt cggcatcggc caggtgggca agagcttccg caacgagatc acccccggca
       61 acttcatctt ccgcacgcgg gagttcgagc agatggagat ggagttcttc gtcaagccgg
      121 gcaccgacga ggagtggcac cagcactgga tcgacgcccg gacgtcctgg tacaccgacc
      181 tgggcatctc cccggagaac ctgcgccact acgagcatcc caaggaaaag ctgtcgcact
      241 actccaagcg gacggtcgac atcgagtaca acttcggctt caccggtggt gacggctggg
      301 gcgagctcga aggcatcgcg aaccgtaccg acttcgacct gggcacccac tcttctcact
      361 cggggacgga cctgtcgtac ttcgagcagg cctccggaga gcgctacctg ccctacgtga
      421 tcgagccggc cgccggcctg acccggtcgc tgatggcctt cctggtggag gcctacaccg
      481 aggacgaggc gccgaacacc aagggcggcg tcgatgtccg caccgtgctg aagctcgacc
      541 cgcggctgtc gccggtgaag gctgccgtgc tgccgctgtc gcgcaacgcc gacctgtcgc
      601 ccaaggcccg cgacctggcc gcgcagctgc gcaaggtgtg gaacgtggag ttcgacgacg
      661 cccaggcgat cggccgccgg taccgccgcc aggacgagat cggtacgccg ttctgcatca
      721 ccgtcgactt cgacaccctt accgacgatg cggttacgat ccgcgagcgc gacaccatgt
      781 cgcaggagcg ggtggccctc gaccaggtgc agtcctacct gggtgcgcgc ctgatcggct
      841 gctagccgga tctcagacca gcgggacgaa gctgaaccgg tagcccatca cgggctcccg
      901 gtgccagccc tcgtggtcac ggtcgatcac ccacatcaca ccggctgccg gcaggaccat
      961 ccgtccgccg atcgccagct gctcttcgag ggcggacggg acgccaccgg cgtccgcgga
     1021 caccaggatg cgatcgaacg gcccggcctc gggcgcgccc agcgtgcctt tgccggccgg
     1081 ctcgatccgt gcccagccca gatcgaaccg ggccaggttc gcgcgcccga actcgaccaa
     1141 ctcggggacg acttccagcc ccagtactga gcctctgggc ccggcgagct gggcgagcag
     1201 ggcggtggtc cacccggagc cgctcccgac gtccagcacg cgggcaccct cgggcacatg
     1261 cagcaactcg agcatgaatt gcacggtcca cggttgggag ttggtggcgc cgtgaccgat
     1321 cggcagtggt tcgtcggctg ctgcccgcgt ccgctgatcg ggcggcagga actccacccg
     1381 tggcaccagc gcgagcgctg aggttacctg gccgtgcacg atgctcatgg cacttctcct
     1441 cgtggtccgt tcctcaccac tcgtcgagac ttcgcgcttc ccgacgctgc cgcctagctc
     1501 acacgcttgg cggctcgcgc gcaagggtct gggtaggctg ttcgggcggt gatgtcgcga
     1561 tttcgtgact ggatcggcgc atggtcccgg aactggggct cgagagacat cgaacagcga
     1621 tatggcgaca tcggaggtga gcatggcgga aacgctgcgc ctggcggcgc cgtccggcgg
     1681 cacggtcgaa gtgtcgaccc cggtcgtgct cgcgccgatg gccggcatca ccaacgccgc
     1741 gtaccgccag ctctgccgcg agcagggtgc cgggctgtac gtatgcgaga tgatcacctc
     1801 ccgcgggatt gtcgagcgca ttcccaagac cttcgacatg ctgcgcttcg acgccgggga
     1861 gaccgtccgt tcggtgcagc tgtacggcgt cgacccgccg gtgctggcca aggccaccga
     1921 gatcctgtgc gccgagttcg gcgtgagtca tgtcgacctc aacttcggct gcccggtccc
     1981 gaaggtgacc cggcgcggcg gcgggggagt gctgccctgg aagcgcgacc ggttggccgc
     2041 gatcctgcgc gccacggtgc gggccgccga ccgctacggc gtcccagtca ccatgaagac
     2101 ccggctcggc atcgacgccg accacctcac ctatctcgac gccggccgga tcgcccagga
     2161 cgccggctgc gcggcgatcg ccctgcacgc gcggacggtg cagcaggcct actccggcga
     2221 ggcggcttgg gagaccattc acaccctggt cgaggcggtg gaccttccgg tgctcggcaa
     2281 cggcgacatc tgggaggcct cggacgcgtt ggcgatggtc gcccagaccg gctgcgccgg
     2341 ggtggtgatc ggacgcggct gcctggggcg tccgtggctg ttccgcgacc tggccgacgc
     2401 cttcgccgga cgggatcggc gcaccctgcc cagcctcggc gaggttgctg cgatggtgcg
     2461 ccggcacgct gaactgctgg ctgagctgta cggcgagaag cacggcctca ccgacctgcg
     2521 caagcacatg gcgtggtact tcaagggctt cccggtcggt ggcgagatcc ggcgcggcct
     2581 ggcgatggtt tcgtcgctgg ccgagctgga cgatcggctg ggccagctag atccgtcggt
     2641 gccgttcccc gaagccgagc tcggcgcgcc ccgcggacgc cagggcacac cgcgggataa
     2701 ggtcaccatg ccctatggct ggctggattc ctgcgagctg ggtgacgccg acatcgccga
     2761 cgccgaatcg gacgcctccg gcggctgacc cgctcgactg ctagccgagc cactccttcg
     2821 cggccgccac cagcgcgcag acgccgaccg aaagggtcgg cgttatgtcc agcctttggt
     2881 gcgcgcggaa accacgacgg gcccgtcacc accacagaag gtggcgcggg cccgccgcga
     2941 ccggctggag ttcaggcgcc ggcgtacggc atcagcgcca tcaacatgac cgcaagtagc
     3001 acgatcgggg ccaggatgcc gagcacggtc gcgaggacgc cccagaggcg tccgcggttg
     3061 accacgatgg cgacgatgcc ggtcacgaaa ccggcgaatc cgaccaggga cacgatgttg
     3121 aggatcatgg ccgggcccgc cgcgttggcg accagcacct cggcgaggta gtcctggtcg
     3181 acggtctcga gtccgctctg ggcgatgacg ctcgccatga ccgccatgac cggctgcaac
     3241 gcgaagatgc tgacgatcag gctcatcgcc accaccgcga gagcgacgat gccaagaacc
     3301 gggctcttct tggccttgac cggcgcaccg gggtagctcg tggtcggata gggctggccg
     3361 taagcgccgt aaggctgctg gccataggca ctcgggtact gctgagccgg ctggcccgtc
     3421 tgcgcgtacg gaccggtgta cgggcccggc gcaggttgcg actgcgggta ggcgccctgc
     3481 gggtactgca ccggcgggta ctgagctgat gggtactggg ctgatgggta ctgtgcgggc
     3541 gggtactggc ccggtgcgta cggcgtcggc gccggctgtc cggtttgtga atactggtcc
     3601 ccagggccgg ggttgggcgg ctgggtacct tcagtctgtg acatggccaa agactagcgc
     3661 tgaaggcccg gcttccagcc cagccgaacc gctatgtcga cgcgactggg ggaccggctg
     3721 cgaagatggt cgacatgcct gaactgcagc cgcgagccgt ggcctgggcc gagaccatgg
     3781 ccgggcatcc cggcgagcga ttcgttcgcg cccacgcaac gtcggggccc tccctcgaac
     3841 tgagggaacc cgtgctgcgc gaacaacggg aggcgtggga ggccgtacac ctcacgccgg
     3901 ggtcggctta cgctctcggg gcaggggagc gtgcccgttc tgaggagccg gacgccgagc
     3961 gcacctgctt cgagcgggac cgcgaccgca tcgtgcattc caccgctttc cgacgcctgg
     4021 ccggcaagac ccaggtggtg gtcttcccga ccgaccacca acggacgcgg ctcacgcacg
     4081 cgctcgaagt ggcgcaggtg gccacgtcga tcgccagggg agtgggtgcc aacgggagcc
     4141 ttgccgaagc gatcgcgctc ggccatgact gtggccacgg gcccggcggt cacgccagtg
     4201 aggacgcctt cgacgcgttc ctgcccgagg gctacgacca cggcccgtgg ggtgctgacg
     4261 tggtccttgc cggcctgaac ctgtgcgccc agaccctgga cggcatccgc aaccactcct
     4321 ggtcccgtcc ggtgccggca acggtggagg gcgagatcgt cagttgggcc gaccggatcg
     4381 cctactgcgc gcacgacctg gaggacgccg ccggcgccgg catcgtcgat ctcgtcgatc
     4441 tgcctgccga gatcgccgga gtctgcggac gcacccggcg gcagcagctg ggcacgttcg
     4501 tccgcgcggt gatcgaaacc gtcgcgagca ccggccaggt cggaatgcgt ccggacgagg
     4561 ccggggcgct ggcgggcctg cgggcgttca actacgagcg gatctacacc cgtcccgact
     4621 cggtcgcgca gtcgcgggcg gtcatcgacg tactacacgc tctggtggag tactacgcca
     4681 agcacccttc tgcaatgccc gccgacttcg tcgacgggaa cgtgcgcggc gcggtcacct
     4741 atgtggccgg catgaccgac cgcttcgctt tcgagaccgc agccgaactg ctgggctggg
     4801 atgccgatcg gctgccgaag ggaatcggtc gcggcgcctg acatgttcct aacactcttg
     4861 gcctatctgg ccttttcagc attgccctgc ccgattcgac gctcggcgtg gcgtggccgg
     4921 ccatgcggct cgacttcggc cagccgatcg gggcggctgg tttggtgccg ccgctcgggt
     4981 actggctgac gatgttcgcc ggccgggtgc tgctcggctg ggttggccga gcggacgggg
     5041 ccgtggccgg tgctgggcgg agccgcgatc ggcctggtgc tggctggcgt cctcgcgatg
     5101 ctgaacacac cagccaccgc gattgcggca gtgctgctgt tcgggctggc cgcggcaccc
     5161 atctatccgc tgctcacgct caccaccgcg gaacgtacgt ccgcggcgat tgtggacacc
     5221 gtggtcgggc tgcgggccgg agcgtcttcg ctcgggcccg caattctccc gctggtggtg
     5281 ggcttggcga tgaaccagtc gatctccgca gtcgcgccgg tgctcctggt cgcctgcctg
     5341 atcgttgccg cgctgcacgc cgtcctgcag ttgcgcagag cggtcgggcg cggacctcag
     5401 atcggctgac cagcccgatg cgtccaggaa ctacaatcac cgcgtggccg gcctgatcaa
     5461 cgaggaggat ctggcgaccg tccgcgagcg ctcgcgaatc gaggacgtcg tcgcctcgtt
     5521 cgtcacgctc cgccgcgccg gcggcggctc gctggccggg ctgtgcccgt tccacgacga
     5581 gaagaccccg agtttccggg tgaccccggc gcgcggcttc tggcactgct tcggctgcgg
     5641 cgaaggcggg gacgtgatca gtttcgtcca gaagatcaac aacgtcggat tcgccgaagc
     5701 ggtcgagtac ctggccgacc gggtcggggt gcagctgcgg ttcagcgatg cgggcggcgc
     5761 ccgggtcgag cccggcctgc gcatgcgcgt gctggaggcg aaccggctgg ccgctgagtt
     5821 ctacaccggc cacctgctca gcgcgaccgg gctgcccggc cgccagttcc tcgacgggcg
     5881 cggcttcgac aagaccgcag ccgagcagtt cggtatcggc ttcgccccga ccggcggacg
     5941 cgagctcggt cagcacctca aagctcaggg cttcagcgac gaggagctgg tcagggccgg
     6001 cctcgtccgc gagaacggct gggatttctt ccagggccgc gtgctctggc cgatccggga
     6061 cgccggccgc tcggttctcg ggttcggggc caggcgcatc ttcgacgacg accggatgcc
     6121 ggccaagtac ctgaacactc ccgagacgat cgtctacaag aagtcgaacg tgctctacgg
     6181 cctagacctg gcccgcggca acatcgccaa gaagaaccag gcggtgatcg tcgagggcta
     6241 caccgacgtg atggcctgcc acctggccgg ggtcgacacc gcggtcgcgt cctgcggcac
     6301 ggcgttcggc gacgagcatt cccggctgct gcaacgcctc ctgggtaccg gtgacgtcag
     6361 cggcggcgag gtcatcttca ccttcgacgg cgacgcggcc gggcaggccg cggcgctcaa
     6421 ggtgttcaag ggcgaccaga acttctccgc gcagacctac gtcgcggtcg agcccagcgg
     6481 gctcgacccg tgcgacctgc ggatccagca gggcgaggcc gcggtccgcg agctcgtcgg
     6541 gcggcgcgtc ccgctgtacc agttcgtgat gcgtaacatc ctcgccgaac atgacctcga
     6601 ccgggtggac ggccgcttgg ccgccctgcg tgcggcggcg ccgctggtga gcagtatccg
     6661 cgacaagagc ctgttcgagc gctacatccg ggagctggcg aagtggctcg gcgaccccga
     6721 cccgaacgag gtgcggcgtg aggtgaacgc caccatgcac cggcgtccgg ccgagcagcg
     6781 ccgccaagcc gaccgccggc cgccagaagt tgcggacgcg gccctgcccg gactgcccga
     6841 accggcgccg agcctgcccg tgcccgatcc gcgcgaccgc cggctggaag ccgaacgcgg
     6901 cacgctgcgg ctgctggtgc gcgcgccgga gaagttcgtc gacgactggt tcgggttggt
     6961 gcccggcgac ttcacccacc ccacctaccg ggcgctgttc gaggtggttc agggtgtcgc
     7021 ggacggtgcc ggcccctgga cgcaacggat gctggcggcc aaccccgatc cggtgctgga
     7081 gagcctggtg atctcgctcg ccgtcgagcc ggtgctgcgg gagccgaccg aggcctacgc
     7141 tcgcgaatac gccgctcagt tgcgagtgca gaccgtctct cgtcagatcg ccgagctgaa
     7201 gtcgaagcta cagcgcacca atccgctggc cgaccagacg gcctacaacc ggatgttcgc
     7261 ccagctggtg aacctggaga aggaccgcaa ggacctggtc gcattggcca ccggcccagc
     7321 gttgtgagtc cctcccgggg aattggtgtt gtcaatttgt tgctccacag atcgtcgtcg
     7381 ggctcctgac atccgccttg ccgccacgca gagtggtggc catgagatcc ctacgttcgc
     7441 tgcacctgcc caccaccgac gaggaagtgg cctgggcccg agccatcgag gccggcgtcc
     7501 tggccgcccg gctgctcgtc accggggagc gtccggtgcc ggctaccgat gccgagctca
     7561 gcgcgctggt cgaagccggg caccgggcca ggcagcggtt cctgctcggc tacgttcgcc
     7621 tggtcgccaa actcgctgcg gccgaggcaa agcgctgcgg gttgaatccc gaggacctct
     7681 gccaggaggg ctttgtcgcc ctctgcgaca ccttgcagcg cttcgaccac acccgctgcc
     7741 ggttctccac ctacgccacc atgcgggtcg ggcagcacct ggcggaggtc ggcgccgccc
     7801 ggctcggcga cctggccatc ccggccagtc gggcgttgcg gttgcgccgg gcacgcggtg
     7861 tcgccgcagc gctcggccag gagcagcaac ggacggttgg agctgcggag gtggccgcgg
     7921 cggtgggggc ttcggcgcgg gacatgtcca ggttgctcgc ctatcgcgcc ccggtgccgg
     7981 tggacgtgct ggccgaggcg atccccgatc ccgatccggt cgacccggac gccggcatct
     8041 acgccgagca gttcggacgc ctggtggccc ggctcgagcc gacccaggcc gagatcgtgc
     8101 gactgcgcta cggactggta accggcgagc agctcgacgt gcagcagacc gcgcggttgc
     8161 tcgggctgag cgagagcagc gtccgtcggc tcgagcggaa ggcgctggcc gcgctgcgtc
     8221 cgatgctgga agctacgccg gcggggcggt actaccgcga accaccagct cggtggacag
     8281 cgtgacgtgg tcggcagcgg cctcgccggc aaggagcgcc agcagcgtcc gcatggctcg
     8341 ctcgccgatt tcgtgcagcg gttgggcgac ggtggtgaga ttggcctgcg cggcgtccgg
     8401 cacgttgtcg aacccgacca cggacaggtc ctcgggtacc cggagaccca ggctttcggc
     8461 gacctcgatg gtccggatgg cggtcaggtc gttcgccgcg aaaaccgcgg tgggccgctg
     8521 ggggagcgag agcaattcca gtgctggacc gcctgcggtc tcggcgcggt agccacccac
     8581 tcgaatcagg ttctcgtcca cgtccagccc ggcagcggcc agtgtccgtc ggtagccctc
     8641 ctcgcggagg agagccgatt gcaggtccgg ccggccggcg aggaagccga tccggcggtg
     8701 gccgaggccg agcaggtgct cgaccgcgga gtcggcgccg ccgaggttgt cgcagtcgat
     8761 cgtcggcagc acggtggtcc cggtgtgcgg gtcgatcgcc accaccggga ttcgtgggtg
     8821 cccgtccact acggtcgggg tgaccaggac cgcgccgtcg atcagggtgc cgctgagccg
     8881 cgacagcgaa cgcatctccc agccttcgtg cccgttgggc ccggtaccgc cggagtgcgc
     8941 gagtagctcg tacccgctgt ccttcgcacc gctggcgagg cccttgagca cctcggtgct
     9001 gaatggctcg aactccgcga ccagaatgcc gagcacgccg gtgcggtgtg cgcgcaggct
     9061 cttggccacc aggctcgact cgtagccgag ctcggcgatg acttggcgga cgcgccgctc
     9121 ggtcagcttc gcgacgccgt agcggtcgtt aatcaccttc gacacggtgg ccaccgaaac
     9181 cccggcagag cgcgccacgt cggtgatggt cacgttccga ctgagttcca ctgcatcacc
     9241 atagcccccc gctgcggatt gtatgtcaat cgttatcgat aacgattgac atttgttgtg
     9301 agccactgca aactcgatcg tatccagtca aggatgacga gaggacaacg aggatgaagg
     9361 cattgcgcaa gcggtccgcc gctgcggttg cgatgatcgg cgcggtcagc ctgctggctg
     9421 cctgcagcac cggtggcggc acggccaccc agcccgcggc caccggcgac accacggcgt
     9481 ctgccgaggc ggtcaccatg agcttctggc acaactccac caccggcgac ggcaagaagt
     9541 actgggagga cacggtcgcg gcgttcacca aggagcaccc gaacgtcacg atcaacatcc
     9601 aggccgtgca gaacgaggac atggacggca agcttcagac ggcgctgaac tccggtgacg
     9661 atccgaccat cttcatggcc cgtggcggcg gcaagctggc cgacgtcgtg gccgccgacc
     9721 aggtgctcga catcaccgac aagatcgacc cggccatcaa gtccgctctc ggcgacggca
     9781 tcttcagcgc cttcaccgtc gacggcaaga tctacggcat gcccacctcc gtactccccg
     9841 gtggcatcta ctacagcaag gacctgttcg cgaaggctgg catcacggcc gtcccgacca
     9901 ctttcgacga gctgaacgcc gctgtggaga agctcaaggc catcgacgtg gccccgatcg
     9961 cgctgggcgg caaggacgcc tggccggctg cccactggta ctacttcatg gcgctgcgca
    10021 actgctccaa ggacgtcatg gacaaggctg cggctgagaa ggacttctcc gatccctgct
    10081 ggctgaaggc cggcgaggac ctcaaggctt tcacggccac cgacccgttc aacaagggct
    10141 tcttgaccac gtcggcccag cagggtgcgg gtagctcagc cggcatgctt gccaaccaca
    10201 aggctgcgat ggaactcatg ggtgcctgga accccggcgt gatcgccgac ctgaccccgg
    10261 acaagaaggc gctgcccgac ctcggctggt tcccgttccc ggccgtcaac ggtggtgccg
    10321 gtgacgcgag cgcgatgatg ggtggctcgg acgggttcgc gtgccgcaag aacgctccgg
    10381 cagcctgcgt cgacttcctg aacttcatgg ccaccaaggc caaccaggaa ggctacgcga
    10441 ccgcgttcaa gacgcttccg gcgaacaagg aagccaaggg cgtcgtcacc gatccggctc
    10501 tgcaagacgt gctgaagtcc tatgacagcg cggcctacgt gatgctgtgg ctggacacca
    10561 tgtacggcca gaacgtgggc aacgccctga acggtggcgt ggtcaacatg ttcgctggga
    10621 agggcgagcc gaccgacatc gttgaggcgg tcaagagcgc ggccgccaag ggctgagcga
    10681 aggacatcga aacctcatga gcgactcagt gaccgcactg ccaaaacgct cgtcgaggtc
    10741 aggatcggag cggggcgccg ccacagcggc gacccgctcc gaccgggcca gggctgaggt
    10801 gcgcaagcgt ttcgagatcg cggtccttgc cggaccggcg atcgtcatgt tcctggcctt
    10861 cgtcatcttc cccgtcttca tggcggccta ctacgggttc ttcaagtgga agggcttcgg
    10921 cgctccgacg aacttcgtcg ggttcgcgaa ctatgtggtg atcttctcag accaggcgtt
    10981 ccgcgacgcg ttggtgcaca acggcttcat cctggtgatg tccttggtga tgcagggtcc
    11041 ggcggccatc gccttcgccc tgctgctgaa ccagaacatc cgcgggcggt cgctgattcg
    11101 cgtgctgatc ttcgtgcctt acgtgatttc agaggtcatc gtcggcaccg gctggagcct
    11161 gatgctgcaa tacaccggcg cagtcaatga tctgctcacc aacatcgggc tgggcgcgct
    11221 ggcgcacgac tggatcgccg acccgagcgt cgccatctgg accctgatgg tgatcatctc
    11281 ctggaagtac atcggcttcg ccgtgatctt gatgctcgcc ggcattcagg gcattcccga
    11341 cgagctttac gaggctgcgg ccattgacgg tgcgtcgtac tggcagattc agcgctggat
    11401 caccctgccg ctgctcggcc ccacgatccg ggtgtgggcg ttcctttcga tcatcgggtc
    11461 gctgcagcta ttcgacctcg tctacatcat ctggggtcag tacgtggcct ccaccgcggg
    11521 cacgtccacc atggccacct acatggtgcg tgagggccgc ttggccggta actacggcta
    11581 cggcaacgcg gtggcggtcg tgatcttcat catttcactg ctcatcgcgc tgacctacca
    11641 gcggttcgtc atgcggcgcg acaccgaggg cgccctcacc ggcgcgtccg gaaggaagcg
    11701 gaagtaatgt cccagacatc cctcaccgcc cgcaccggcc ccagtcgccg gatcgcgacc
    11761 agcccatccg gcgccacctg gggcagtccg accacctacc tggtcgccac cgtgctgatc
    11821 gcggtctgca tcgcacccgt gatctacatc atcctcggcg gcttccgcag caactcccag
    11881 atcacggtca atccgtccgg gtggcccgac ccttgggtgc tcaccaacta cacggcggtg
    11941 ctcaccagct cccttttctg gcagcaattc accaactcgg tgatctcggc tgtctcgacc
    12001 acggtcggca ccgtcgcgct cgccctgatg gccagctttg tgatcgcgcg ttacagcttt
    12061 gccggccgca acgcgatgta cgcgcttttc gctgccggac tgatgttccc gctgaccgtg
    12121 gcgatcaccc cgctgtacct gttgatcaag aacctggggc tcaccgacaa cctgctcggg
    12181 atcatccttc cccagatcgc gttcgcgctg ccgatgaccg tgatcatctt ggtgcccttc
    12241 ctggccgcga ttcccaagga gctggaggaa gcggccgcga ttgacggggc cagccggctg
    12301 aagttcttcg ttcgaatggt gctgcccctg tcgctgcccg gtgtgatcac caccggcatc
    12361 ctcaccttcg tgaacgcctg gaacagctac atgctgccgc tgttcattct caacgattcc
    12421 aagatgtaca cccttccgct cggggtgcag gcgttttcct ctgagcattc ggtggacacc
    12481 gcggcggtgc tggccttcac ctcgctgtcg atgctgccgg cgttgttgtt cttcagcgtg
    12541 ttcgaacgcc ggattgtcgg cgggctgacc ggcgcggtca agggctgagc ccgcgccaca
    12601 ccccaattca cccaacagtt gaaggagacg gacgtcgtgt ccaccatcgt ggccgggctg
    12661 ccggcgttcc cagcggtctc tgcgcgcgtc cgcgacctgg tcgacggtat gacgctgccg
    12721 gagaagctgg cccagatcgt cggctactgg ctcgaccagg gcggcaacgt ggtcgcgccg
    12781 atgcagggcg aactgggtaa ggccggagcg accggcgagc tcgccgccgt caccgcggac
    12841 gggatcggcc actacacccg ggtctacggc acgcgtccgg tcgagccggc tgcacgcgcc
    12901 gcctggctgt gggacgagca gcgacgattg cagcgcgaga cccggcttgg catcccggcg
    12961 ctggtgcacg aggagtgcct gaccgggctg gccgcctggc aggccaccac gttcccgaca
    13021 ccgctggcct ggggggcttc cttcgacccg gaactggtgc aacaggcagc ggcggcgatc
    13081 ggtgagtcga tgcgttcgct gggcgtgcac cagggcttgg ccccggtact ggacgtgatc
    13141 cgcgacccgc gctggggtcg ggtggacgag tgcatcggcg aggaccccta cctggtcggg
    13201 acggtcggga cggcctacgt ccgcggcctg cagtcggccg gggtgcacgc cacgctcaag
    13261 cacttcctgg gctactccgc ctccatcgcc ggacgcaacc acgcgcccgt ccacgtcggg
    13321 ccgcgggaac tggctgatgt cttcctgccg ccgttcgaga tggcgatcct ggacggcgcg
    13381 gccgcttcgg tgatgaactc ctacaccgac atcgacggca ttccggtagc cgccaacccc
    13441 gacctgctca ccgaagtgct gcgcgagcgg ctgggcttca ccggggtcgt ggtcagcgac
    13501 tacttcgcgg tggccttcct gcaggtgatg cacgcggtgg ccgcggatcg cggcgaggcg
    13561 gcggcgcttg ccttgatcgc gggcatcgac gtcgaactgc cctccggcga cgcttatctg
    13621 gagcccttgg ctgagatggt gcgcaccggt gcgctggatg aggcctacgt cgatcgcgcg
    13681 gtgttgcgga cgctggccca gaaggaggcg ctcggcctcc tcgagccgga cgcgttcgcc
    13741 gaggatgcgc cggcggcgat cgacctcgat ccgccggagc accaggcgnn nnnnnnnnnn
    13801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13861 nnnnnnnnnn nnnnnnnnnn nnnnnnnntg gtgcccggct gcgcggtcga aggcgccgac
    13921 accagcggaa tcgccgcagc ggtcgcgacc gcgaccgacg ccgacgtcgc gatcgtggtc
    13981 gtgggtgacc aggcggggct cttcggccgc ggcaccgtgg gcgagggcaa cgacaccgac
    14041 agcctggacc tgcccggggt gcagcgcgag ttggtcgagg cggtcgtggc caccggcacc
    14101 ccggtggtgc tggtgctgct gaccggacgg ccctacgcga tcggctgggc gctggacggc
    14161 ccggggccgc gtccggcggc cgtggtccag gcgttcttcc ccggcgaagg cggtggcctg
    14221 gcgatcgcgg acgtgctgac cggcgcggtc aatccgtccg gacggctgcc ggtgagcctg
    14281 ccgcgctcgt ccggtgcgca gccgtactcc tacctgcaac cgattctcgg cggaccgtcc
    14341 gaggtgaccg cgaccgatcc gacgccgttg cgtccgttcg gcttcgggct gacttacacc
    14401 acgttcgact acaccgacct ggtggtggac ccggctgttg ccgcgggcgg caacttcacc
    14461 gcggcggtca ccgtgaccaa caccgggcgg gtcgtcgggg ccgaagtcgt ccagctctac
    14521 ggacgggacg tgcgcgcttc ggtggtgcgt ccggtggtgc aactgctcgg ctacgcccgg
    14581 gtggagctgg cggcggggga gagccgacgg gtgaccttcg acgtgccgac cacgcgcttc
    14641 gccttctccg accggcggct ggtgcgggtc gtggagcctg gcgaggtcga ggtttgggtg
    14701 gcgtcgcacg cggccgcgtc cgagctggtg cagagcgttg gcgacaccaa cggctcgatt
    14761 ccgagcagcg accggcgagc gtcggcggtg ccgggaacgg ccacttcgag ggcgagcctg
    14821 gcgttcaccg gcgaggtgca cgaggtgacg gtcgccgatc gtcgcgtggt cggagtccgg
    14881 gtcgacgtgg catgacccga gtcgacaacc cgatcctgcc cggctgtcac ccggacccgt
    14941 ccatctgccg ggtggacggg acgtactacc tggtcacgtc cactttcgag tacttcccag
    15001 ggttgccgat cttcgccagc accgacctcg cccactggga gctgatcggt cacgtggtga
    15061 cccgacctga gcagctcgac tatgccggga tcgcctcttc gggtggactg tacgcgccca
    15121 cgctgcgcca tcacgacggt cgatactggg tggtctgcac cctggtcgac caacaggatg
    15181 ccagccgcgg cggcaacttc ctgatgaccg cgaccaaccc cgccggtccc tggtcggacc
    15241 cggtctggct ggacgcggcc ggcatcgacc cgtcggtgtt cttcgacgac gacggccggg
    15301 tctggctgca cgggacccgg ctggccgccg agcccgaatg gcacgatcag accgaggtct
    15361 gggtgcgcga gttcgacccg gtcacgatgg cgctggtcgg cccggaaacc gtgatctgga
    15421 cgggcgccgt gcgcggcgcc gtctgggcgg aagggccaca cctgtaccga gtggacggca
    15481 cgtactacct gctcgcggcc gagggcggaa ccgagttcca ccacgcggtt tcggtggctc
    15541 gcgcggacgc ggtcaccggg ccctttgtgg gcaacccggc caaccccgtg ttcacccacc
    15601 gccacctcgg ccgtgaggtc gacgtggtcg gagcgggcca cgccgatctg gtgcaggcac
    15661 ccgatggcag ctggtgggca gtgctgctgg cgatgcggcc ctacggcggc taccactaca
    15721 acctcggccg ggagacgttc ctggtgccgg tcacctggca ggacggttgg ccggtgttcg
    15781 cgcctggcgt gggccgcatc ccggcccagg tggagttgcc caccgctgcg gcagtcccgc
    15841 tcagtcccga gcagggcggc gcgtccggtg tggtgctgcc ggacgatcca cgctggacgt
    15901 cactgcgtgg cccggccagc gctttcgccg agccggacgg ggacggctgg cggctgcgac
    15961 tgcgcccgac cacgctcgcc gaacccggca ccccggcgtt tctcggcgtc cgccagcagc
    16021 accgcaacct gaacctgcgg gtcacgctgc acgcaccgct gagcgccggc gaagaggccg
    16081 gcatcgtggt gcgccagtct gagctggacc acgtccggct cttccttcgc ccggataccg
    16141 atcggcacgg gattgttcag gcggtccacc gtcgcggcgg ggtcgagact gtgctcggtg
    16201 agtgggccac ggcggctccg gtgggcgacc ggctggtatt gacagtcgag atacgcaacc
    16261 aggcgtacgc cctggtcgcc cagctcgacg ctggccccaa gacccggctt ggcgtggtcg
    16321 acggcagcca gctcgacagc gtcgccaccg gtgggttcct cggcctctgg ctgggcgtct
    16381 acggcaccag caacggtgcc gagtccacaa cgacggtagc ggtcgattcg gtcgagtatc
    16441 tgcccgacta ggctcgtccg cgtggaacgc attctcgggt tgttgctggt gctcacgctg
    16501 agcggttgtt cgttgctcgg cagttcgagc tgggatcagg tgaaagtggc gttcagcggc
    16561 gcggacaaag gtgactacac cctcgtggtc acgcccaagc aggccatcta caccatcgac
    16621 ggcaaggcga cgtccgaaga cctcccggag ggcgcctgga ccgcgttggc cacgggtgtc
    16681 gccgcgctcg gtgcccgcac cagcaaggag tgcgatggcc agcgcctgaa gatccaggcg
    16741 ttgtccggcg gcgacgtgtt gcagaccttc gaggcgacca gttgcgacgg cggtgaagct
    16801 ctggagcagg cgaagggcgt gctcgaccaa ctcatcgcgc agcttcgctg agggggctgg
    16861 catgggcatt ccgctgatca tcgacaccga caccgcgcag gacgactgcg tcacgatcct
    16921 gctggcgctg ctcgacccgg ccgccgacct gcgcgcgatc accatggtcg ccggcaacgt
    16981 gggcttcgag cagcaggtgg ccaacgccca cctcaccctg aacgtggccg gcaagctcgg
    17041 ccaggtgccc gtccacctcg gctgtcggca gccgatgctg cgtcagtggg agtcggccga
    17101 ggatgtgcac ggcgacggtt cgggtggtct ctcgatggac cttgccgcgg ttaccaccga
    17161 gcccgagcac gcggtggacg cgttgcttcg gctcacgtcc gagtcgccgg gggagttgag
    17221 catcatcgcg atcggtccgc tgaccaacct ggccaccgcg gtggtgatgg acccgggctt
    17281 cgccaagcgg gtgaagagcc tctacatcat gggtggctcg aacaacggcc gcggcaacat
    17341 cacggccagc gccgaattca acttctacgt cgacccggag gctgcccaga tcgtgctgga
    17401 cgcgggcttc cgcgacctgc tgatcctgcc ttgggacccg gtaacgctgc gcgacgcgac
    17461 catcgatcgg gccggctacg accgactctg cgccctcggg acgccgctgg cgcgcttctt
    17521 caggcgcatg tgcgacgcga cgatcgacta cgacgagtcg gtgggggtga acgggtcgac
    17581 ccatccggat tcgctgacgc tcgcggtgct gcttcacccg gaactgatcc ttgccgaagc
    17641 gccctaccgg gtggacgtgg agacccgttc ggaactcacc cgcggctatt cggccatggc
    17701 gtgggagaag ttcgacctgg tgcccaatgc ccgggtggtc gaggcgatcg acactgcggc
    17761 gttcttcggc cagctggccg acctgctgcg caccgagacg gtgcccgacc tgccgatagc
    17821 cgggatcgac tagaggtcga gcaggtggtt cagggtggcg accacaccca tcgccacgtt
    17881 ggacggcgcg atgtaggcgg cggtgtcgaa cacgtccgga tgcgagttgg ccatggcgaa
    17941 ggagtgctcg gcttgggcga gcatctccag atcgttgagg tagtcgccga aggccacggt
    18001 ctgcgcccgg gtgacgccca gcgcctcctg tagccgggcg agcgcgacgc ccttgttgac
    18061 gccgacgctc atcacgtcta cccagtgggt ggtcgcaacg accacctggg catccgggca
    18121 cagtccggcg atcttgtcgg cgacctcgtg cggatcccca aagacgtaga tggagagctt
    18181 gagggcgttg gtggtgtgcg cgagcaggtc gtcggtagcg gtgaggttgg cgtagtagtg
    18241 cccggctgct tcggtgaagc ggcggtcggc gcgctcggcg taggacagtt ccttgccgga
    18301 cacgaccagg cccaggtcaa cgcgtccgct ggccttcagt tcgcggacgc cgcgaaccac
    18361 ggcggcgacc gtttccggcg ccagatagct ggtgatcacg tcgacgccgt gatggacgac
    18421 gtaggtgccg ttgtcggcga tgtaggcgtc gatctcgtcc gccacggacg cgaacgcgtg
    18481 cagcagcgaa gcgtaggggc gtccgctggc gggagcgaac acgatgccgc gttcgcgtag
    18541 ccggtgcagc acgccggcca gttcggctgg gacggcgccg gccgcgtcca gcagggtgcc
    18601 gtccaggtcc gctgccacca ggcggacgtc gatcgggtgt tcgggaagga tcggatcggg
    18661 aaggacgctc accgctcgac tgtaaacccg gccgatgcca gtgcctaacc gaggctgacg
    18721 ggcaccaggc ggtcggaatc cggatacgcg acgtaggcga tccggttgcc cgcgtcggcg
    18781 cagaggacgc cgccgccttt ctctacctcg atcgttcggg ccgtcccggt gagcaggttc
    18841 tgctcgtaga tgccgtagtc gtgcaggagc tagagccgct gccgcttggt gtcgatggtc
    18901 agttcccgtc cgggccgcag ggcgagagtg tcctgccgct tcagggtggt cgaccggagc
    18961 agggcgactt ggccccactc atcgagggcg tacagcaggc cggcggcggg atcggtggcc
    19021 agcgccgcga agtgcccgga cggataggtg ccgacgcgtt tgttggtccg ggtgtcgtag
    19081 gccaacagcg actcgtcgtc gccgctgctg gcgacgaaca gacgatggtg cttggtgtcg
    19141 atcgccagat ccaccgggtc cttcccggcg tccatctcgg ccaccgtgtc gcctggcgca
    19201 acctggatgt cgcatggcga gcagagtgca gttccgttgc tgaacgctgc gaaggtccgc
    19261 agctaggagg cgcgggattc gccgtggcgg gccaggtact cagtgagcgc ctcacggacg
    19321 aggtccgact ggcgccgtcc ggactcctcc gccaggtgct cgagttgttc cttggtgacc
    19381 tgtgacacgc gaaggttcag cacgggcgag tgggccccgg gtgcggtgag ggagggccgt
    19441 ccgccacgcc gctcgagacg atcggcgatc tccttcgcgc gtgcttccgt cagcggctcg
    19501 ccgccgacga cgaactcctc ggtgtcgagg tcgatgtcac ggattgtgac gtcaggcccg
    19561 aaaccagagc ggaaacgctc gggtcgctgc tgctcggtca tcggttcctc cgctcccttg
    19621 gcattgcgtg caggactgcg aagccctcgc cacggtcgtc cgccactatt cccagttcaa
    19681 tcaccagacc tcgcccgtcc gtgccgacgt agatcgccac gtcttcgtcc atatggtcga
    19741 acaccgcgtt ggtgagcgcc tcccggatcc gagagctcga gagcttgcgc ctgcgggcgg
    19801 tgctcgtgat ccggatctgt gcgagaccca ttgtgtctta caaaaccact tgcgccaagg
    19861 gcgctctcga agcgagatcc tgatctattg gccctagag
//
LOCUS       DAOPRQ010000050        19506 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_2381_length_19506_cov_12.456480, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000050 DAOPRQ010000000
VERSION     DAOPRQ010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 19506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19506
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2381_length_19506_cov_12.456480"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            56..1552
                     /locus_tag="PKJ61_07810"
     CDS             56..1552
                     /locus_tag="PKJ61_07810"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014847087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HOC13769.1"
                     /translation="MLTNTLPAISLSATVGKEPGVLVVGLAETSEGELLVGLPDELAK
                     AFSKALGSDVTTIAKQLGASAKTGKTVLLAGAGGLKLVVVGIGPIDVTPEQVRRAAGA
                     AVRLIATLPADAPKTVTISLETSEPKVIKGAAEGALLGAYTYLPAKAGTAGIGEIVLV
                     SAKPKAEGKNAVELAKITAGAVVLARDWVNTPSNDLYPESFAEAAKAVARNAKLSVEV
                     WDDKALEREGFGGLMAVGGGSSRKPRLVKVAYAPRGARFHLALVGKGITFDTGGINLK
                     PADAMYTMKADMAGAASVLAAVSAIAQLGLKIRVTAWAAMAENMPSGTAYRPSDVLTI
                     YGGKTVENGNSDAEGRLVMADALVKCCEEQPNLIVDVATLTGACVIALGDRVAGLMAR
                     DDQTADLVLDAAEAAGEQFWQLPIPEDARPKLESKVADLKSTGDRPGGALTAAAFLSE
                     FVPTEIPWAHLDIAGPAFNSAAAYDYTPADATGMSVRTLVALAHVLAN"
     gene            1555..3111
                     /locus_tag="PKJ61_07815"
     CDS             1555..3111
                     /locus_tag="PKJ61_07815"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOC13770.1"
                     /translation="MTANYDLVVVGAGAAGLLAAVAARRLGQNVLVVEADRLVGGSTA
                     LADGRLWLPGQQGGKSSADSVADAEEYLAALLGEPTVASSLERRSAFATAAGRLVRWL
                     TSSKIPLNPVRNAGDHHPELAGAKTHGRVLQTQPMDRKALGEWAEKLRPEPASAGGPL
                     ARILPKRRITETTGESLVGQLLLRATANGVEIWLETPVVDLLGDADAITGVVVHRDGA
                     DVEVHAAMVLLASGGFERNQELREEYLPLPTEAAWSTSVETDTGDAIRLAVAKGAATA
                     ELDAAWWRPVIMVDGVAHPLNDAREKPHSLIVDSVGDRFFDETIIDDAMGRAVYDRGF
                     GVRAVPSFLIMDNRHRQQVALGPWTAGNTPRKAIESGEIVRASTLNDLAESLGIDRAG
                     LIGTVVRFNGFAAKGKDLDFARGESGVTREGGKRRNPSLGKVDKQPFWAVRVYPGDAG
                     TKGGLLIDADSRVLRADGQAIGGLYACGGVAASIFKRTSPGPGAALGESLVQAFLAVT
                     SGSRPADSTD"
     gene            complement(3306..3635)
                     /locus_tag="PKJ61_07820"
     CDS             complement(3306..3635)
                     /locus_tag="PKJ61_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13771.1"
                     /translation="MGAVAWFSRKRRKGTESKREGVDPATIAHLEQFLSSRRGVEAFI
                     EQPTTVTKATLLLIAHDGEWTRRSVHNVEWARSFATSHKLPAYDAGVVGYPQRMRDYN
                     ARRRSGG"
     gene            3701..5563
                     /gene="sucB"
                     /locus_tag="PKJ61_07825"
     CDS             3701..5563
                     /gene="sucB"
                     /locus_tag="PKJ61_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014847094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase, E2 component,
                     dihydrolipoamide succinyltransferase"
                     /protein_id="HOC13772.1"
                     /translation="MSTPVQLPALGESVTEGTISRWLKSVGDHVEVDEPLVEVSTDKV
                     DTEIPSPVAGVLLEILVAEDGVAPVGATLAVIGEPSEAGGAPAAEAPAPQAAPAPEPT
                     AAPAPAPVAAQPVAQPAPAAAATPTPAAPAAGGTDVLLPQLGESVTEGTISRWLKQVG
                     DSVEVDEPLVEVSTDKVDTEIPAPAAGVLLEIRVAEDAVAPVGAVLAVIGAPGAAPAP
                     APAPAAPAPEPAPAPVAPPAPPAPAPAPSAPTAPAPAPAPAPVAPPAPAAPAAPEPAA
                     APAEPAARRAVTEAADSTYVTPLVRKMAEANDVDLNSISGTGVGGRIRKQDVLAAAEA
                     RRAEEAAKAAAAAAAVPVATPAPAAPAAPSAPSPAPSTAAPSADANLRGTTQKMSRLR
                     ATIAKRMVESLQVAAQLTATVEVDLTAVSKLRGRVKDEFKAREGVGLSYLPFIAKASA
                     EALKQFPVVNATIDTEAGTISYADAEHLGIAVDTEKGLLVPVIKDAGDLNVTGLAKKI
                     ADLAARTRANKVTPDELSGGTFTVTNYGSAGTLFDTPIINQPQVAILGTGALVKRPMV
                     VKDPNLGEIVAVRDMMYLSLSYDHRLVDGAVAARFLSFVKARLEEGDFGGELGQ"
     gene            5560..6342
                     /gene="lipB"
                     /locus_tag="PKJ61_07830"
     CDS             5560..6342
                     /gene="lipB"
                     /locus_tag="PKJ61_07830"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002513994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HOC13773.1"
                     /translation="MREQVPDRTPVGFRYLGIADDPATLTDYHAAWQLQREVHAEVAA
                     GELGPQVLYVEHPPVYTAGRRTEPHERPFDGTPVVDVDRGGKITYHGPGQLVGYPIVF
                     LPRGVGVVDYVRRVEEALIRLLADYGISSGRVPGRTGVWLAAAEGKPERKIAAIGIRV
                     SRQTTMHGFALNVTSDLADFGRIVPCGIADAGVTSMADELTQRSLSLPKGPSLLEVGR
                     ALEPHLVEMLRFEPFEQSPDLHPEPAAPAELDRPTVRYGLLN"
     gene            6397..6984
                     /locus_tag="PKJ61_07835"
     CDS             6397..6984
                     /locus_tag="PKJ61_07835"
                     /inference="COORDINATES: protein motif:HMM:NF025670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP repeat protein"
                     /protein_id="HOC13774.1"
                     /translation="MRSSSEWCATTNSLPTVAVLTFGKSGCTSGRLITQATKGVAGSP
                     ATKTQFGYSLAVDDDHLAVGAIRDDVPGARDTGAVQLFRLAGSKVSPVKRISQSSAGV
                     PGKSEPEDWFGSAVAFGRVCPGVTGLVVGASSEVLRNDGEDDWPEGSAWLIPLKAPSA
                     CRAVQFLAYLVSGHPGPAKPWRPRMVSSSVSPAAD"
     gene            7157..8092
                     /gene="lipA"
                     /locus_tag="PKJ61_07840"
     CDS             7157..8092
                     /gene="lipA"
                     /locus_tag="PKJ61_07840"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HOC13775.1"
                     /translation="MTDTQPRRLLRLEVRNAQTPIERKPAWIKTTATMGPDYRELQQL
                     VKDEGLHTVCQEAGCPNIFECWQDREATFLIGGDSCTRRCDFCQIESRKPSGYDTAEP
                     VRVAESVKTMGLRYATVTGVCRDDLEDEGAWLYAETIRQIHALNPGVGVEMLAPDFSG
                     RAELLQQIFDTRPEVFAHNVETVPRIFRRIRPGFNYEQSLGVLTMAREQNLVTKSNLI
                     LGMGETREEISQALRDLHAAGTELVTITQYLRPSVRHHPIDRWVTPDEFDELAAEATA
                     IGFVGVLSGPLVRSSYRAGRLYRQAMAARAQQAAN"
     gene            8109..8873
                     /locus_tag="PKJ61_07845"
     CDS             8109..8873
                     /locus_tag="PKJ61_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4191 domain-containing protein"
                     /protein_id="HOC13776.1"
                     /translation="MASEKAKELAAKQKAEVKAAKLAKKNSTNPRDWPWYKQYWQTYK
                     VTAEVDPQLPWLLLGSFFGVLVVVAVLGGVFLPPWWIWLLLGILGGVTAALWVLLIRA
                     KKATYVRYAGQPGSAEVALSMLDAKKWFHTAAITATRQLDAVHRVVGPAGIVLIGEGA
                     PARVKQLLASEAKKHEQVAYGVPVTSILMGDGEGQVPLAKLADHIKKLPKKMEAVQIT
                     DVKARLRALDAVRPKVPVPRGPMPSMKGVNRAMRGR"
     gene            8870..10672
                     /locus_tag="PKJ61_07850"
     CDS             8870..10672
                     /locus_tag="PKJ61_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002549006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOC13777.1"
                     /translation="MSLANLVNAERVSIAFGTRILLDEVSLGLDNGDVVGVVGRNGDG
                     KTTLLQLLVGTRTPDSGQVVRNRSASIGYLGQAEDFAPDASVREVAVQGRPDHAWAAD
                     RSSRAVVTHLLGTLDLNRPVAELSGGERRRVSLASILLAGHDLLVLDEPTNHLDVEAV
                     AWLADHLNLLQASGTAMLVVSHDRWFLDAVCNRIWEVHDAAVDAYDGGYAAYVLARAE
                     RVRQAAANEARRQNLLRKELAWLRRGPPARTSKPKFRIDAANVLIEDVPPARDSLKLV
                     EFATSRLGKDVFDLHGVTHRLPDGRTLLNHLDWSIGPGDRIGIVGVNGSGKTTLLRLL
                     TGELSPQAGKVKQGRTLRVGHLSQAVTELDDSERVLDSVQQLQRQTRLATGREVSATN
                     LLEDFGFTGNRLVTRIGELSGGERRRLQFLRLLLSEPNVLLLDEPTNDLDIDTLTVIE
                     DYLDTWPGTLIVVSHDRYFLERTCDVTYALLGDGTCVLLPGGVDEYLQRRTAARQTRP
                     VTPVETSAKHSATVPQPADAARRRQARKDLARVENQLSKTAAQIDQLHAEMARHAADY
                     PRLVALGETLDEAVARQEELEGQWLELAEQAGDE"
     gene            10689..11546
                     /locus_tag="PKJ61_07855"
     CDS             10689..11546
                     /locus_tag="PKJ61_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13778.1"
                     /translation="MNWRTLTGALLSVTAGLLAPVAILAGWVQTLASDTDQVVASYAP
                     LAQAPQVQQLLSERLTDAISDQVPLGHTQTMHGLINRTVTSALGSEQFQQAWTTSLRI
                     AHGEVSALLAGGTSTLTVTDGLIELRLAPFVDLLKQPLTDAGVPMLDRLPEVTSSIPL
                     ASIDPALVPSLQAGHRVLVALAAWLPWFVLAVTLLAWWAWPGLRPGLAWVGLSLFAGT
                     ALAWTVVAGLARLWVGQVAPPIAPTAELLLGTTLAPLRAPGLAVALFGLVLAFVGAFA
                     RPAETRTHD"
     gene            11587..>12014
                     /locus_tag="PKJ61_07860"
     CDS             11587..>12014
                     /locus_tag="PKJ61_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13779.1"
                     /translation="MGLLGLGVAIAVCLVALVRIGAGQLRRRNGGTVTVGVVKSVEVR
                     YQAAGEGPTMVQRTPVVEFQDASGTIRQFQNAHPVDCPDAGGFGYEQRRDRHTVAQQH
                     GRSGTCHRPAHATSLVSARSSSSRTGSGSGGGSGSAVSSSS"
     assembly_gap    12015..12114
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(12290..12607)
                     /locus_tag="PKJ61_07865"
     CDS             complement(12290..12607)
                     /locus_tag="PKJ61_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13780.1"
                     /translation="MSVRNDRGQASLVLVLAVPALLAAAEQWRSYVADAIQAADGRDP
                     EDALDRLRALLTTDPTALGGSLVSHRLGNWLLRTTPRCPRRPYAGPEPASSSRSPLAI
                     STR"
     gene            complement(12604..14097)
                     /locus_tag="PKJ61_07870"
     CDS             complement(12604..14097)
                     /locus_tag="PKJ61_07870"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOC13781.1"
                     /translation="MIAVLGTMMLFGCTPVPQPTPPPSTSTTAPQPSTSAPVSSSPTA
                     TPELATVYQLPADRDSAEWGTPVASMVGKKLSVARLNLSREGWRVDLMGAYRLASDRI
                     LVEFRLNADNAIRSDLKVWGDPEFDEWFHARDEAGGGFSSAFWEFSDVRLSVAGDDLS
                     YRPVRTATNHCLCTMSNSHVRGFGDFPAYVVLTAPANATSVSLALRDVGSFEDVAVSP
                     AVPQRSVVPLGYGYQLRLQSWTRPTPGKVAARVSVEAPAAGLAGSPFRQRRYDQLGAA
                     PWLKLTDYTMAYQLLLALDSSGTYGGWPDAGGCQSCAEAKPVRAGQAVDFEVTLPDPG
                     GSGLMAAPTVGWPLTAVPVEPDAPRSEQAVWYQGRKVTTGATIKDGQIDLDSAVLFAV
                     DKATLTTTANSVLDRTAKTLQDQEKRTVRVVGHTDSTGSTAHNLDLSKRRAQAVREAL
                     TKRLGAGWTFMVEGSGESQPRIKESGLSGAELDRARALNRRVEVSFS"
     gene            complement(14213..14437)
                     /locus_tag="PKJ61_07875"
     CDS             complement(14213..14437)
                     /locus_tag="PKJ61_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13782.1"
                     /translation="MNTAIEIYAKTTARLQQVLRSERGQGTIEYVGILFIVAAIVAAI
                     LTFVKGADGSTLGQQLLDAIGKAITDVTGG"
     gene            complement(14488..14970)
                     /locus_tag="PKJ61_07880"
     CDS             complement(14488..14970)
                     /locus_tag="PKJ61_07880"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOC13783.1"
                     /translation="MLGNAASAGLALRNGIEMVSREMDAPAAEEFGEVSRRLSLGTPL
                     EDALRDLAQRLPSPELAVLVKTIVVQAKAGGGLVTALTSIANTLEDRRELQRELKTTT
                     TGAVFIGWLVVGIAMGSVLIMNLITPGALDAMVQTVPGQIVLFVLGHVIINRITRIEA
                     "
     gene            complement(14913..15311)
                     /locus_tag="PKJ61_07885"
     CDS             complement(14913..15311)
                     /locus_tag="PKJ61_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13784.1"
                     /translation="MVVLVLLLVAAAAVYDSGSRRQQIVLASTGDQDTSRLIRLRRRA
                     NQYLRTTRRGLSLDQRLAGASVALGPADFILLVAGGTLLVGLICQPLLGYLGAAVIMF
                     GTAFLADRWLAGCWATPPAPVWRCATGSKW"
     gene            complement(15519..15740)
                     /locus_tag="PKJ61_07890"
     CDS             complement(15519..15740)
                     /locus_tag="PKJ61_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13785.1"
                     /translation="MAPIIYSWLPDEMAPKPGAVARQLGWVATVADGVLFAITMIVVA
                     ALGYWLAARTGVVTTLTRRMDGEKADLKK"
     gene            complement(15900..16544)
                     /locus_tag="PKJ61_07895"
     CDS             complement(15900..16544)
                     /locus_tag="PKJ61_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13786.1"
                     /translation="MEMQTVPEDQMEAFTAKVADVVGESVNVDEVSDAQWAQLSALQT
                     PKTVTEVTNQQVALVGAPWAVDANGEPIATHYELADGALTQVVDINADTAFPVTADPE
                     WWWWAWTATSCVADIASFIFAAAKLAKILIKVSGLIKKSATVARWVTKLGGAEKTLKA
                     IYYAAKGFLESGRVGKYLSNTTVLALSAFAASGLTLLGDALGIGSCVSLVRALL"
     gene            complement(16553..17722)
                     /locus_tag="PKJ61_07900"
     CDS             complement(16553..17722)
                     /locus_tag="PKJ61_07900"
                     /inference="COORDINATES: protein motif:HMM:NF025001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOC13787.1"
                     /translation="MRNSTVLAGRSVALVLETPDLDVVVVDGGLFDPLALEVVRELGV
                     TAPLVAVVVVGTPSLSLAAVVAAGGRAMITSPLSLEEVQSQLDHAAAWSRSLRQHVSG
                     DAVGVRGERGTILALAGAKRGVGVSVLATVLATQAAALGSTVCLVDLDLRAGDLASLC
                     GVSPRRTIADLAGLVGDLTIRGIREVAVEVPAGFTLLAAPDRVEQSEDATGPAVRQIL
                     NELRRHFSLVIVDTGAALGDVRAAALELSDEVILVADCELISIRAGRRMLDSWARLNV
                     CQPADARLVVNRVAKRREVQPDLVSKLIKLPLTKAVPELTDELEEPINSGTLTSERMP
                     NMEAAARQLLVNCGVSGEVRATPTKRRLGSRAKAEVGSAALELPVFVRSPTALSR"
     gene            17733..18479
                     /locus_tag="PKJ61_07905"
     CDS             17733..18479
                     /locus_tag="PKJ61_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012921270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HOC13788.1"
                     /translation="MFSKRRPDVPTGFFEVEAAGLRWLRVPEGVSVAAVVSVAPDEIV
                     LERVAQRQPTHSAAARFGRRLAVTHDAGAAMFGSPPTGWSGDGFIADLPLSLRPTQRW
                     GEFYAEQRVLPYARQSARSGSLSSSGLDLIETLAERLASGEFDDAAPPARIHGDLWAG
                     NVLFSGDSATLIDPAAHGGHRITDLAMLELFGLPYLSEALAAYADASQWLPSGWRDLI
                     GLHQLHPLLVHAALFGGSYGEQAVRVARRY"
     gene            18820..>19506
                     /locus_tag="PKJ61_07910"
     CDS             18820..>19506
                     /locus_tag="PKJ61_07910"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOC13789.1"
                     /translation="MSASLFCSGLAASYGARPLFSGLELTLASGVTALVGPNGSGKTT
                     LLRILAGEHQPDAGTVRLAPMDATIGYLPQSLPEPKESALDYALRRTGVGPAGVRFER
                     AAAALADGGDPDDYAHALERWLALGGADLETRLAATLDQLGLEPALDRPLGTLSGGQA
                     ARVALASILVSQYDVLLLDEPTNNLDAAGLKAMTGFVTGQRSPVLIASHDRAFLDDVA
                     TSVVELDLHQQ"
BASE COUNT         3125 a         6778 c         6512 g         2991 t
ORIGIN      
        1 gcgggctagc cttagaacgc gcccggatcg ggcacctcgg aaaggaccca cagccgtgtt
       61 gacgaacact ctgcccgcga tcagcctgtc cgcaacagtc ggcaaggagc ccggggtgct
      121 ggtcgtcggg ctggccgaaa cctccgaagg cgagctgctg gtaggtctgc ccgacgaact
      181 cgcgaaagcg ttcagcaagg ccctgggcag cgatgtcacc accatcgcca agcagctcgg
      241 cgcctcagcg aagaccggca agaccgtcct gctggccggt gcgggcgggc tcaagctggt
      301 ggttgtcggc atcggcccga tcgatgtcac ccccgagcag gtccggcgcg cggcgggcgc
      361 tgccgtccgg ctcatcgcca cgctgccagc ggacgcgccc aagaccgtca ccatcagcct
      421 ggagacgtcc gaacccaagg tgatcaaggg ggccgccgaa ggcgcgctgc tgggcgccta
      481 cacctaccta ccggccaagg cgggcaccgc cggcatcggc gagatcgtgc tggtttcggc
      541 gaagcccaag gcagagggca agaacgccgt cgagctggcc aagatcaccg ccggcgccgt
      601 ggtgctggcc cgcgactggg tgaacacccc cagcaacgat ctttaccccg agtccttcgc
      661 cgaagccgcc aaggccgtgg cccgcaacgc caagctgagc gtagaagtgt gggatgacaa
      721 ggcactcgag cgcgaaggct tcggtggcct gatggccgtc ggcggcggtt ccagccgcaa
      781 gccgcggctg gtcaaggtcg cctacgcacc ccgtggcgcc cggttccacc tcgcgctggt
      841 gggcaagggc atcacgttcg acaccggcgg catcaacctc aagcccgcgg acgccatgta
      901 caccatgaag gccgacatgg ccggggccgc gtccgtgctc gctgcggtgt ccgcgatcgc
      961 gcagctcggt ctcaagatcc gggtcaccgc gtgggcagcg atggccgaga acatgccgtc
     1021 cggcaccgcc taccggccct cggacgtgct caccatctac ggcggcaaga ccgtcgagaa
     1081 cggcaactcc gacgccgagg gccgcctggt gatggccgac gcgctggtga aatgctgcga
     1141 ggagcagcca aacctgatcg tcgatgtcgc gactctcacc ggtgcctgcg tgatcgccct
     1201 cggcgaccgc gtggccggcc tgatggcccg cgacgaccag accgccgacc tggtgctgga
     1261 cgcggccgaa gccgccggcg agcagttctg gcagctgccg atcccagagg acgcccggcc
     1321 gaagctcgag tcgaaggtgg ccgacctgaa gtccaccggc gaccgtcccg gtggcgcgct
     1381 gaccgcggcc gctttcctct ccgagttcgt gccgaccgag atcccgtggg cgcatctcga
     1441 catcgccggt ccggcgttca actccgctgc cgcctacgac tacaccccgg ccgacgccac
     1501 cggcatgagc gtgcggacgc tggtcgcgct ggctcacgtg ctggccaact gaggatgaca
     1561 gccaactacg acctggtggt ggtcggcgcc ggcgccgcgg gcctgctcgc tgccgtggcc
     1621 gctcgccgcc tcggccagaa cgtgctggtg gtcgaagccg accggctggt cggcggcagc
     1681 accgccctcg cggacggacg gctgtggctg cccggccagc agggcggcaa gtcgtccgcc
     1741 gattcggtcg cggacgccga ggagtacctc gctgcgctgc ttggcgagcc gaccgtcgcg
     1801 tccagcctcg aacggcgctc ggccttcgcg actgcggccg gacgcctcgt ccgctggctg
     1861 acgtcgtcga agatcccgct gaaccctgtc cgcaatgccg gcgaccacca ccccgagttg
     1921 gccggcgcca agacgcacgg acgggtgctg cagacccagc cgatggaccg aaaggcgctc
     1981 ggcgagtggg ccgagaaact gcggcccgag cccgcgtccg ccggcggccc gctggcccgc
     2041 atcctgccca agcggcggat caccgagacc accggcgagt cgctggtcgg ccagttgctg
     2101 ctgcgcgcca ccgcgaacgg cgtggagatc tggcttgaga ccccggtcgt cgacctgctc
     2161 ggcgacgccg acgcgatcac cggcgtggtc gttcaccgcg atggcgccga cgtcgaggtg
     2221 cacgccgcca tggtgctgct ggccagcggc ggtttcgagc gcaaccagga gctgcgtgag
     2281 gagtacctgc cgctgcccac cgaggctgcc tggtccacga gtgtcgagac cgacaccggc
     2341 gatgcgattc gactggcggt cgccaagggc gcggccacgg ccgaactgga cgcggcgtgg
     2401 tggcgtccgg tgatcatggt ggacggggtc gcgcacccgc tcaatgacgc cagggagaag
     2461 ccgcactcac tgatcgtcga ctcggtcggc gaccgcttct tcgacgaaac catcatcgac
     2521 gacgccatgg gccgtgcggt ctacgaccgt ggcttcgggg tgcgcgcggt cccgtccttc
     2581 ctgatcatgg acaaccgcca tcgccagcag gtcgcgctgg gtccgtggac ggccggcaac
     2641 acaccccgca aggcaatcga gtccggggaa atcgtccggg ccagcacgct gaacgacctg
     2701 gccgagtcgc tcggcatcga tcgggccggg ctgatcggca ccgtcgtccg attcaacggg
     2761 ttcgccgcca agggcaagga tctcgatttc gcacgcggcg agtcgggcgt aacccgtgag
     2821 ggcggcaagc gccgcaatcc gagtctcggc aaggtggaca agcagccgtt ctgggcggtc
     2881 cgggtctacc ccggcgacgc cggcacgaag ggcgggctgc tcatcgacgc cgactcccgc
     2941 gtcctgcgtg cggatggcca ggcgatcggc gggctctacg cgtgcggtgg cgtggcggcg
     3001 tccatcttca agcgaacgtc gcccggcccg ggcgctgcgc tgggcgagtc gttggtgcag
     3061 gccttcctgg cggtcacctc gggctcacgt ccagccgaca gcaccgattg aggctccgaa
     3121 gggttcaacc cagattcgac cgtttatgtg agcccgtgcg cgtccccgga tcggatcaac
     3181 ccaagttttc ccggttatcc ggcccgaaaa tcgtcggata accggctgtt tctgggttgg
     3241 gctgcggcga cggccaacgg gcccgttcat aaccagctga acttgggttg aacgcccggc
     3301 gagactcagc cgccggagcg ccgccgggcg ttgtaatcgc gcatgcgctg cgggtacccg
     3361 accaccccgg cgtcataggc cgggagcttg tgcgacgtgg cgaagctgcg cgcccattcg
     3421 acgttgtgga cgcttcgtcg cgtccattcg ccgtcgtgag cgatcagtaa cagggtggct
     3481 ttcgtgaccg tggtcggctg ctcgatgaac gcctccacgc cacggcggct ggagaggaat
     3541 tgctcaaggt gcgcgatggt cgccgggtcc acgccctcgc gcttggattc ggtgcctttg
     3601 cgccgctttc gtgagaacca agccacggcg cccattatgg ccgctcgttt cggggctagt
     3661 ctgaaccctg aaccgatcag agagctaagg agccaacgtt atgtccactc ccgtccagct
     3721 gccggcactc ggggaaagcg tcaccgaagg cacgatttcc cgctggctga agagtgtcgg
     3781 cgaccatgtc gaggtggacg agccgctcgt tgaggtctcc accgacaagg tcgacaccga
     3841 aattccgtcg ccggtggccg gggtgctgct cgagatcctg gtggccgagg acggcgtggc
     3901 gccggtcggc gcgaccctcg cggtcatcgg cgagccgagc gaggccggcg gtgctcctgc
     3961 cgccgaggcg ccggcacccc aggccgctcc tgctcccgag cccaccgctg cacccgcacc
     4021 ggcccccgtg gcggcccaac ccgtggccca gccggctccg gctgccgctg caactccgac
     4081 ccccgcagcc ccggccgccg gtggcacgga tgtcttgctt ccccagctcg gtgaatcggt
     4141 caccgaaggc actatttcgc gctggctgaa gcaggtcggt gactcggtcg aggtggacga
     4201 gccgctggtc gaggtctcca ccgacaaggt cgacaccgag attccggcac cggcggccgg
     4261 tgtgctgctg gagattcgag tggccgagga cgcggtcgct cccgttggtg cggtgctcgc
     4321 cgtgatcggc gctccgggcg ctgcacctgc cccggccccg gccccggccg ccccggcacc
     4381 cgaaccagca ccggctcccg tggccccgcc cgcacctccg gctccggcgc ccgcaccttc
     4441 cgctcccact gcacctgctc cggcccccgc gcctgctccg gtggcaccgc ccgctcccgc
     4501 ggctccggcc gcacccgagc ccgccgcggc accggccgag ccggctgccc gccgcgcggt
     4561 caccgaggcg gccgattcga cctacgtgac cccgctggtg cgcaagatgg ccgaggccaa
     4621 cgacgtcgac ctgaactcga tctcgggcac cggggtcggc ggacgcatcc gcaagcagga
     4681 cgtccttgcc gccgctgagg ccaggagggc cgaggaagcc gccaaggccg ccgctgccgc
     4741 ggccgccgtt ccagtcgcca cgccggcgcc ggccgcgccg gcggccccgt ccgccccgag
     4801 ccctgccccg tccacagcgg caccgagcgc ggacgccaac ctgcgcggca ccacccagaa
     4861 aatgagccgg cttcgcgcca ccatcgccaa gcggatggtg gagtcgctgc aggtcgccgc
     4921 ccagctcacc gccaccgtcg aggtcgacct gaccgcggtc tcgaagctgc gcggccgggt
     4981 gaaggacgag ttcaaggcgc gcgaaggcgt cggcctcagc tacctgccgt tcatcgcgaa
     5041 agccagcgcc gaggcactga agcagttccc ggtggtgaac gcgaccatcg acaccgaggc
     5101 cggcacgatc agctacgcgg acgccgagca cctgggcatc gcggtcgaca ccgaaaaggg
     5161 cttgctggtg ccggtgatca aggacgccgg cgacctcaac gtaaccggcc tggccaagaa
     5221 gatcgccgat ctagccgccc gcacccgcgc caacaaggtg accccggacg aactgtccgg
     5281 cggcaccttc acggtcacca actacggctc ggcgggcacg ttgttcgaca ccccgatcat
     5341 caaccagccc caggtcgcga tcctcggcac cggcgcgctg gtgaagcgtc cgatggtggt
     5401 caaggatccg aacctcggcg agatcgtcgc ggtgcgcgac atgatgtacc tctcgctcag
     5461 ctacgaccac cgcctggtgg acggcgccgt cgctgcccgc ttcctcagct tcgtgaaggc
     5521 gcggctggaa gagggcgact tcgggggtga gctcggccag tgagggaaca ggtgcccgac
     5581 cgcaccccgg tcgggttccg ctacctcggc atcgcggacg acccggcgac gctgaccgac
     5641 taccacgccg cgtggcagct tcaacgtgag gtgcacgcgg aggtggccgc cggtgagctg
     5701 ggtccgcagg tgctgtacgt ggagcatccg ccggtctaca cggccggacg ccgcaccgag
     5761 ccacacgagc gtcccttcga cggcaccccg gtcgtggacg tggaccgcgg cggcaagatc
     5821 acctaccacg ggcccggcca gctggttggc tacccgatcg tgttcctgcc gcgcggggtg
     5881 ggcgtggtcg actacgtccg ccgggtcgag gaggcgctga tccggctgct ggccgactat
     5941 ggcatctcat ccggacgggt gcccggacgc accggcgtgt ggctcgcggc agccgagggc
     6001 aagcccgaac ggaagatcgc agcgatcggg attcgggtgt ctcggcagac cacgatgcac
     6061 ggcttcgcgc tcaacgtgac cagcgatctc gccgacttcg gccggatcgt cccttgtggc
     6121 atcgccgatg ccggcgtcac ctcgatggcg gacgaactaa cccaacgttc cctgagcctg
     6181 ccgaagggcc cgtctctgct cgaagttggc cgcgcactgg agccgcacct ggtcgagatg
     6241 ctgcgcttcg agcccttcga acagtcgccg gatctgcatc cggaaccggc agcaccggcg
     6301 gagctggacc gcccaaccgt ccgctacggt ctcctgaact gaactcccac cgcgagccgg
     6361 tcaacccgct gagaactccc tgaagttctt cccggcatga ggagctcatc cgagtggtgt
     6421 gccacgacga attcgttgcc caccgtggcg gtgctcacct tcggaaagtc cggctgcact
     6481 tcaggcaggt tgattaccca ggccaccaag ggagtggccg gaagtccagc caccaagacc
     6541 cagttcggct acagcctggc cgtcgacgac gaccacctcg ctgtcggcgc gattcgggac
     6601 gacgtgcccg gggctcggga caccggcgcg gtgcagctgt tccgcctcgc cggcagcaag
     6661 gtcagcccgg tcaagcgaat tagccaatcc agtgcgggcg tacccggaaa gtccgagccc
     6721 gaagactggt tcggcagcgc ggtggccttc ggtcgagtgt gcccaggggt gaccgggttg
     6781 gtggttggcg cttcgagcga ggtactccga aacgacgggg aggacgattg gcccgaaggg
     6841 tccgcctggc tgatccccct gaaagctccg tccgcgtgcc gagcggttca gttcttggcg
     6901 tacttggtgt ctggtcaccc cggaccagcc aagccgtggc gtccgagaat ggtctcatca
     6961 tcagtgtcgc cggccgctga ctaggcgcgg ccggcgctcc agacacgtca aacttcgctc
     7021 ccgaaaggga ctcgcgctcc cgaactatcg ggagcaaggg tcccattcgg gagcgaagtt
     7081 gctgaagtgg cggaagcgag cggacgcggg ccgcggggcc gcggggccgc gggacaaggc
     7141 cggataggat tggcgggtga ccgacaccca gccgcgccgg ctgctccggc tcgaagtccg
     7201 caacgcgcag actccgatcg aacgcaagcc ggcctggatc aagaccaccg cgaccatggg
     7261 gcccgactac cgcgagctgc agcaactggt gaaggacgag ggtctgcaca ccgtctgcca
     7321 ggaagccggc tgtccgaaca tcttcgagtg ctggcaggat cgtgaggcca ccttcctgat
     7381 cggtggcgat tcctgcaccc gccgctgcga cttctgccag atcgagtcgc gcaagccgtc
     7441 cggttatgac accgccgaac ccgtccgcgt cgctgaatcg gtcaagacga tgggcctgcg
     7501 ctacgcgacg gtcaccggcg tctgccgcga cgacctcgag gacgagggcg cctggctgta
     7561 tgccgagacc atccggcaga tccacgcgct taacccgggc gtcggcgtcg agatgctggc
     7621 ccccgacttc tccggacgcg ccgaactgct gcagcagatc ttcgacaccc gtccggaggt
     7681 gttcgcgcac aacgtcgaga ccgtcccgcg gatcttccgc cggatccgtc ccggcttcaa
     7741 ctacgagcag tccctcggtg tgctgaccat ggcccgcgag cagaacctgg ttaccaagtc
     7801 gaacctgatc ctcggcatgg gtgagacccg cgaagagatc tcccaggcgc tgcgcgacct
     7861 gcatgccgcc ggcaccgagc tggtcacgat cacccagtac ctgcggccgt cggtgcggca
     7921 ccatcccatc gatcgctggg tgacgccgga cgagttcgac gagttggccg cagaggccac
     7981 cgcaattggc tttgtcggcg ttctgagtgg accattggta cgttcttcct accgtgccgg
     8041 ccggctgtac cggcaggcca tggcggcccg cgcccagcag gccgccaact aagacccgga
     8101 gcaccgaaat ggcaagcgag aaggccaagg aactggccgc caagcagaag gctgaagtca
     8161 aggccgcgaa gctggcgaag aagaattcca ccaacccccg ggactggccc tggtacaagc
     8221 agtactggca gacctacaag gtcaccgcag aggtcgaccc gcagctgccc tggctgttgc
     8281 tcggctcctt cttcggcgtg ctggtcgtag tggcggtgct cggtggcgtg ttcctgccgc
     8341 cgtggtggat ctggctgctg ctcggcatcc tcggcggcgt cacggccgcg ctctgggtgc
     8401 tgctcatccg cgccaagaag gccacctacg tgcgctatgc cggccagccc ggctcggccg
     8461 aagtcgcgct ttcgatgctg gacgccaaga agtggttcca caccgccgcg atcaccgcca
     8521 cccggcagct ggacgccgtc catcgcgtcg tcggaccggc gggcatcgtg ctgatcggtg
     8581 agggcgcacc cgcccgggtg aagcagctgc tggccagcga ggcgaagaag catgagcagg
     8641 ttgcctacgg cgttccggtg acctcgatcc tgatgggcga cggcgagggc caggtgccgc
     8701 tggccaagct ggccgaccac atcaagaagc tgcccaagaa gatggaagcg gtgcagatca
     8761 cggacgtgaa ggcgcggctg cgcgcgctcg acgcggtccg gccgaaggtg ccggtgccgc
     8821 gcggcccgat gccgtccatg aagggcgtca accgggcgat gcgcgggcgc tgagcttggc
     8881 caacctggtc aacgccgaac gcgtctcgat cgcattcggc acccgcatcc tgctggacga
     8941 ggtcagcctc ggcctcgaca acggcgatgt cgtgggcgtg gtcggccgca acggcgacgg
     9001 caagacgacc ctgctgcagc tcctcgtggg cacgcgtacg cccgactccg gtcaggtggt
     9061 gcgcaaccgg tccgcgtcca tcggctacct cggccaggcc gaggacttcg cgcccgacgc
     9121 cagcgtccgc gaagtcgcgg tgcagggccg tcccgaccac gcctgggccg ccgaccggtc
     9181 ttcgcgggcc gtggtcacgc atctgctcgg cacactcgac ctgaaccggc cggtggccga
     9241 gctgagcggc ggtgagcggc ggcgggtgag cttggcgtcc atcctgctgg ccgggcacga
     9301 cctgctggtg ctggacgagc cgaccaacca cctggacgtg gaagcggtcg cctggctggc
     9361 agatcacctg aacctgttgc aggcgtccgg gacggcgatg ctggtggtca gccacgaccg
     9421 ctggttcctg gacgcggtct gcaaccgcat ctgggaggta cacgacgccg ccgtggacgc
     9481 ctacgacggt ggctacgcgg cgtacgtgct ggcccgcgcg gaacgcgtcc ggcaggctgc
     9541 ggcgaacgag gctcgccggc agaacctgtt gcgtaaggag ctggcctggc tgcggcgtgg
     9601 cccgccggcg cgcacgtcca agccgaagtt ccggatcgac gcggcgaacg tcctgatcga
     9661 ggacgtgccg ccggcgcgcg actcgctgaa gctggtggag ttcgcgacgt cccggctggg
     9721 caaggacgtc ttcgacctgc acggcgtgac ccaccggctg ccggacggac ggacgctgct
     9781 gaaccacctc gattggtcga tcggcccggg cgaccggatc gggatcgtcg gggtgaacgg
     9841 ctcgggcaag accacgctgc tgcggctgct gaccggcgag ctgtcgccgc aggccgggaa
     9901 agtgaagcag gggcggacgc tccgggtggg ccacctttcc caggcggtca ccgagctgga
     9961 cgacagcgag cgggtgctcg actcggtaca gcagttgcag cgacagacgc gcttggcgac
    10021 cggacgcgag gtgtcggcga ccaatctgct ggaggatttc gggttcaccg gcaaccggtt
    10081 ggtgactcgg atcggcgagc tttccggcgg cgagcggcgg cggctgcaat tcctgcgcct
    10141 gctgttgtcg gaaccgaatg tgctgctgct ggacgagcca accaacgacc tcgacatcga
    10201 cacgctcacc gtgatcgagg actatctcga cacctggccg ggcaccctga tcgtggtctc
    10261 gcacgaccgc tacttcctgg agcgcacctg cgatgtgacc tacgcgctcc tcggcgacgg
    10321 cacctgcgtc ctgctgcccg gcggcgtgga cgagtacctg cagcgtcgca ccgccgcccg
    10381 acagacccgg ccggtcacgc ctgtcgagac ctcggccaag cactccgcaa cggttcctca
    10441 acccgcggac gcagcccgcc ggcgccaggc ccgcaaagac ctggccaggg tcgagaacca
    10501 gctgagcaag accgccgcgc agatcgacca actacatgcc gaaatggcta ggcacgccgc
    10561 cgactacccc cgcctggtcg ccctcggcga aaccctggac gaggcggtgg ccaggcagga
    10621 ggagctggaa ggtcagtggc tggagctggc tgagcaggcc ggagacgaat gaaccacggt
    10681 aagcgaccat gaactggcga acgctgaccg gcgctctatt gagcgtcacg gccgggctgc
    10741 tcgccccggt ggcgatcctc gccggctggg tgcagacgct tgcttccgat accgatcagg
    10801 tggtcgccag ctacgcgcca ttggcccagg ctccccaggt gcagcaactg ctgagcgagc
    10861 ggctgaccga cgcgatctcc gaccaggtgc cgctcgggca cacccagacc atgcacggtc
    10921 tgatcaaccg gacggttacc agcgcgctcg gcagcgagca gtttcagcaa gcctggacca
    10981 ccagccttcg gatcgcccac ggcgaggtca gcgcgctact cgcgggtgga accagcaccc
    11041 ttaccgtcac cgacgggctg atcgaactgc ggctggcgcc gttcgtggac ctgctcaagc
    11101 agccgctgac cgacgctggg gtgcccatgc tcgaccgtct tcccgaggtc accagctcga
    11161 ttccgctggc cagtatcgac ccggccctgg tcccgtcgct gcaggcgggg caccgagtgc
    11221 tggtggccct ggcagcttgg ctgccgtggt tcgtcttggc tgtgacgctg ctcgcgtggt
    11281 gggcttggcc ggggctgcgt cccgggctgg cctgggtggg gctgagcctg ttcgcgggca
    11341 cggcgctggc ctggacggtc gtggccggac tggcccgcct gtgggtcggt caggtggctc
    11401 caccgatcgc acccaccgcc gagttgctgc tgggaacgac cttggcgccg cttcgtgccc
    11461 ccggccttgc ggtcgcgctg ttcggactgg tgctggcttt tgttggggcc ttcgcgcgtc
    11521 cggctgaaac ccgaacgcac gactgagctg agatcggcaa aggagaccgg ggtggacttt
    11581 cgggtagtgg gcctgctggg cctgggggtc gcgatcgcag tatgtctggt ggcgctggtt
    11641 cggatcggcg ccggccagtt gcgccggcgg aacggcggca ccgtcaccgt gggcgtggtg
    11701 aagtcggttg aggtgcgcta ccaggccgca ggtgagggcc cgacgatggt gcagcgaacc
    11761 cccgtggtcg aattccagga tgcctccggg accatccgcc agttccaaaa cgcacaccct
    11821 gtcgactgtc cggacgctgg cggtttcggc tacgagcagc ggcgcgaccg gcacaccgtt
    11881 gcgcagcagc acggccggtc cgggacctgc caccggccgg ctcacgccac cagcctggtg
    11941 tcggcgcgga gcagttccag ccgcaccgga tcgggctccg gcggcggctc cggctctgcc
    12001 gtctcctcgt cctcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntcctcg
    12121 tcctcgtcgg gatcctgcgg cggctcaggg gtgagctcag ccaccaggtc gctccagccc
    12181 aaacaatcgc cgtggtaacg cagcccaagc cgtgagcctc gccagcaggc gccgcgctgg
    12241 agctccacca cggcggtggc ggcggcgacc ggtcgggtgg aggtcgagtt caccgtgtcg
    12301 aaattgcgag tggagaccgc gaactcgatg ccggctccgg cccggcgtac ggtcggcgcg
    12361 gacacctcgg agttgttcgc agcagccagt tcccgagccg gtgcgagacc agcgatccac
    12421 caagagcggt ggggtcggtg gtgagcagcg cgcgcagccg gtccagcgcg tcctctggat
    12481 cacgtccgtc ggcggcctgg atcgcgtcgg cgacatagct gcgccactgc tcggctgcgg
    12541 ccagcagggc ggggacggcg agcaccagca ccagtgaggc ctggccgcga tcgttgcgaa
    12601 cactcaactg aaactcacct ccacccttcg gttcagcgcg cgggcacgat ccagttcagc
    12661 gccactcaaa ccggactcct tgatccgcgg ttggctctcg ccgctgccct caaccatgaa
    12721 ggtccagccc gcaccaaggc gcttggtcag cgcctcgcgc accgcttgcg ccctgcgctt
    12781 cgaaaggtcg aggttgtgcg cggtggagcc ggtgctgtcg gtgtgtccga ccactcggac
    12841 cgtccgtttc tcctgatcct gcagcgtctt ggcggtgcga tccagcacgc tgttggctgt
    12901 tgtggtcaaa gtggccttgt cgacggcgaa caacacggca ctgtcgaggt cgatctggcc
    12961 gtccttgatc gtcgcgccgg tcgtgacctt gcgtccctgg taccagacag cctgttcgct
    13021 gcgcggtgca tcgggctcga ccggcaccgc agtgagcggc cagccgaccg tgggagctgc
    13081 catcagcccg ctcccgccag ggtcaggcag cgtcacctcg aaatcaaccg cctgaccagc
    13141 ccggacgggc ttggcttccg cacacgactg gcatccaccg gcgtctggcc agccgccgta
    13201 ggtaccgctc gaatcgaggg ccaacagcag ctggtaggcc atggtgtagt cggtcagctt
    13261 gagccagggg gccgccccaa gctggtcgta gcgacgctgc cggaacggcg agcccgcgag
    13321 cccagcagcc ggagcctcca ccgaaactcg ggcggccacc ttgcccggcg tcggacgcgt
    13381 ccacgactgc aggcgtagct gatagccgta gcccagcgga accactgacc gctgcgggac
    13441 cgcggggctg accgcgacgt cctcgaagga gcccacgtcg cgcagtgcga gggagacgct
    13501 ggtggcgttc gccggagcag tcagcacgac gtaggccgga aagtcgccga agccgcgcac
    13561 atgcgagttc gacatcgtgc acaggcagtg gttggtggcg gtgcgcaccg ggcggtagct
    13621 caggtcgtcg ccggcgacgc tcaggcgcac gtccgagaac tcccagaacg cactgctgaa
    13681 gccaccgccg gcttcgtccc tggcatggaa ccactcgtcg aactcgggat ctccccagac
    13741 tttcaggtcg gagcggatcg cattgtccgc gttgagccgg aactcgacca gaatccgatc
    13801 ggacgccagc cggtaggcgc ccatcaggtc cacccgccag ccctcccgag agaggttaag
    13861 cctggccacg gagagcttct tgccgaccat ggacgctacc ggcgtgcccc actcggcgct
    13921 gtcccgatcg gctggtagct ggtagaccgt ggccaattcc ggcgtggccg ttgggctcga
    13981 gctgaccggt gccgacgtgc tcggctgcgg cgcggttgtc gacgtgctcg gagggggggt
    14041 cggctgcggg acgggagtgc aaccgaagag catcatcgtg cccagcaccg caatcatccg
    14101 ccgcattgtt cgacctcctc gcaccagcaa ctcgcaccta atctcggcat tggctctcca
    14161 caaccgtagc cgggcacccg cgtggtcgcg ggtgcccggc taggcggggg gcttaaccgc
    14221 cggttacgtc cgtgattgcc ttgccgatgg catcgagcag ctgctggccc agcgtggaac
    14281 cgtcggcacc cttcacgaag gtgaggatcg cggccacgat ggcggcgacg atgaacagga
    14341 tgccgacgta ctcgatggtg ccctggccgc gctctgagcg gagcacctgc tgcagacggg
    14401 cggtggtctt tgcgtaaatc tcgatcgcgg tattcatgac gttcctctca cttcgtcccg
    14461 agggcagcga agatcacggc gaccacgtca cgcctcgatc cgagtgattc ggttgatgat
    14521 cacgtggccg aggacgaaca ggacaatctg gcccggcacg gtctgcacca tcgcatcgag
    14581 tgcccccgga gtgatcaggt tcatgatcag cacagaaccc atggcgattc ccaccaccag
    14641 ccagccgatg aagaccgcac cggtggtggt ggtcttcaac tcccgttgca gttcgcggcg
    14701 atcctcgaga gtgttggcga tgctggtcag cgcagtcacc agcccgccgc cggccttggc
    14761 ctgcaccacg atcgtcttca ccagcacggc gagttctggg gacggcaacc gttgcgcgag
    14821 gtcgcgcagg gcgtcctcca ggggcgtgcc caggctcagc cgacggctga cctcaccgaa
    14881 ctcctcggcc gcgggagcgt ccatctcccg gctcaccatt tcgatcccgt tgcgcagcgc
    14941 cagaccggcg ctggcggcgt tgcccagcac ccggccagcc agcggtcggc cagaaaagcg
    15001 gtaccgaaca tgatcaccgc cgctcccagg taccccagca gcggctggca gatcaatccc
    15061 accagcagcg tcccgccggc gacgagcaga atgaaatccg cggggccgag ggcgacactg
    15121 gcgccagcca accgttgatc gagactcaat ccccggcggg tggtgcgcag gtactgattg
    15181 gcccggcgcc gcaggcgaat cagccggctg gtgtcctggt cgccggtgga ggccagcacg
    15241 atctgctgac gccggctgcc cgagtcgtag acggcggccg ctgccaccag caacagcacc
    15301 agcaccacca aggtggcgcc gatgatcgct agagggtcca ccgggtgccc ccgtcggtaa
    15361 agaccatcgg caccgactcc ccactggcca ccagccgctc ggccaggtcg gcgggcagcc
    15421 gggacggcat ggggtttggg catggtgcac atacttccag cagggcccct caggccccac
    15481 agatcggttg tcacctactc atgcagcaat cccgtccctt acttcttcaa gtcggccttc
    15541 tcgccgtcca ttcgccgcgt gagcgtggtt acgacgcctg tcctcgccgc tagccagtag
    15601 ccaagtgccg ccacgacgat catcgtgatc gcgaacagca caccgtcggc cacggtggcc
    15661 acccacccca gctgtcgagc gactgcacca ggtttgggcg ccatttcgtc cggcaaccag
    15721 gagtagataa tcggtgccac cacaagcacc acggtcagaa gccctccgaa gatactcttc
    15781 gcgcgattcg ccgccacgag gacgacagcc cacaccgcga agacaagccc gacaagcgtt
    15841 cccagagcga gagcgctacc catcgctgac tcccttcata gatcgggttg gacgcttcat
    15901 cacaacagcg cccgcactag cgacacgcag cttccgattc caagtgcgtc acctaggaga
    15961 gtaagccccg atgccgcgaa agctgacaga gcgagaaccg ttgtgtttga gaggtacttg
    16021 cccaccctgc cgctttcaag gaatcccttc gctgcgtagt agatggcctt gagagtcttc
    16081 tcagcaccac caagcttcgt gacccaacga gcgacggtcg cgctcttctt gatcaggccg
    16141 ctgaccttga tgagaatctt ggctagcttg gccgctgcga agatgaatga ggcgatatcc
    16201 gccacgcagg aggttgcggt ccacgcccac caccaccatt ctgggtcagc ggtcacgggg
    16261 aacgcggtat ctgcgttgat gtcgaccacc tgggtgagcg ctccgtcagc caactcgtag
    16321 tgcgtcgcta tcggttcgcc gttggcgtca accgcccacg gcgcccccac cagagctacc
    16381 tgctggttgg tcacctcagt cacggtcttg ggcgtctgga gcgcactcag ctgggcccac
    16441 tgcgcgtcgc tgacctcatc gacgttcaca gactcgccaa ccacgtccgc aaccttggcc
    16501 gtgaacgcct ccatttggtc ttcgggcacg gtctgcatct ccacgggagc aatcaccgcg
    16561 acagagccgt cgggctgcgc acgaagactg gtagttcgag cgcggcgctg cccacttcgg
    16621 ccttcgcccg gctacccagg cgacgtttgg tcggcgtcgc ccgcacctcg ccggacaccc
    16681 cgcagttgac caacaactgg cgcgcggcag cctccatgtt gggcatccgc tccgaggtca
    16741 aagtgccgga gttgatcggt tcctcgagtt cgtcggtgag ttccggcacg gctttggtca
    16801 gcggcagctt gatcagcttc gagaccaggt ccggctgcac ctcgcgccgc ttggctactc
    16861 ggttcaccac caggcgggcg tctgcgggct gacacacgtt cagccgggcc caggagtcga
    16921 gcatccggcg tccggcgcga atgctgatca actcgcagtc ggcgaccagg atcacctcgt
    16981 cggacagctc gagtgccgca gctcgaacgt cgccaagagc tgcaccagtg tcgacaatca
    17041 ccaacgagaa gtgacgacgc agctcgttga ggatttgccg caccgccggg cccgtggcgt
    17101 cctcggactg ctccacccga tcaggcgcgg ccagcagggt gaagccggcg ggcacttcca
    17161 cggccacttc gcggatgccg cggatggtca gatcaccaac cagcccggca aggtcggcga
    17221 tggtgcgacg ggggctgacc ccacacaggg aggcgagatc gccggcccgc aggtcgaggt
    17281 cgaccaggca gaccgtcgag cccaaggctg cggcctgggt ggccagtacc gtggcgagaa
    17341 ccgatacccc aacaccacgc ttcgcgccgg cgagggccag gatcgtacct cgttcgcccc
    17401 tgacgccaac ggcgtcgccg ctgacgtgct ggcgcagcga gcggctccat gcggccgcgt
    17461 ggtcgagctg ggattgcacc tcctccagcg acagcggtga ggtgatcatg gcccggccgc
    17521 ccgcggcaac caccgcggcc aggctgagcg acggcgtccc gaccaccact accgcaacca
    17581 gcggtgcagt cacgccgagt tcgcggacga cttcgagcgc gagcggatcg aagaggccac
    17641 cgtcaacaac caccacgtcg aggtcgggtg tctccagcac cagcgcgacc gaccgcccgg
    17701 cgaggacagt actattacgc aagaatcgga cggtgttcag caagcgccgg cccgatgtac
    17761 cgaccgggtt cttcgaggtg gaggctgccg ggctgcgctg gttacgggtg cccgaagggg
    17821 tatcggtggc cgcggtggtg tcggttgctc cggacgagat cgtgctcgaa cgggtggcgc
    17881 agagacagcc gacgcactcg gccgcggccc gcttcgggcg gcggttggcc gtcactcacg
    17941 acgcgggcgc ggccatgttc ggttcaccgc cgaccggttg gtcaggcgat ggtttcatcg
    18001 cggacctgcc gctttcgctg cggccaaccc agcgctgggg tgagttctac gccgagcagc
    18061 gggtgctgcc gtatgcccgc cagtccgcac ggtcgggcag cctcagctcg tccgggctgg
    18121 acctgatcga aacgctggct gagcggcttg cgtccggcga gttcgatgat gcggcgccgc
    18181 cggcgcggat ccacggcgac ctgtgggccg gcaatgtcct gttcagcggc gattcggcga
    18241 ccctgatcga cccggccgca catggtggcc atcggatcac cgatctggcg atgctcgagc
    18301 tcttcggcct gccctacctg agcgaggctc tcgccgccta cgcggacgcg agccagtggc
    18361 tgccgtccgg ctggcgcgac ctgatcgggc tgcaccaact gcatccgctg ctggtgcacg
    18421 ccgcgctctt cggcggcagc tacggcgaac aggccgtgcg cgtcgcccgg cgctactgaa
    18481 ctccggtggg cgccagttcc atcacgatga actcggtctc ggcgcccagc accatccgac
    18541 gctcgcgtcc gatgtcgcga aagccgctgc cagctctctc gcgatcacgg ttcccaatct
    18601 gccagccgcg cgggccgtac gggagatcac ggacaaaacg ccctgtagat cgtcagatag
    18661 ccgtgaagag cggccggaac ggcaatcgac agggcatttt gtccggccgc ggccccaacg
    18721 acaccggctc cggattgact cggcggcgtc tctcgggtca cgctggacgt atgtggaact
    18781 gacctctcgc cctgtccaac cagcgattga ggaagttccg tgtctgcgtc cctgttctgt
    18841 tccggcctgg ccgcgtccta tggcgcgcgt ccgctgttct ccggtcttga gctgaccctc
    18901 gccagcggcg tcaccgccct ggtcgggccg aacggctccg gcaagaccac cctgctacgc
    18961 atcctcgccg gcgagcatca accggacgcg ggtaccgtcc ggctcgcccc aatggacgcc
    19021 acgatcggct acctgccgca gtcgctgccc gagcccaagg aatctgccct tgactacgcg
    19081 cttcgccgca ctggtgtcgg gccggccggc gtccgcttcg agcgtgccgc cgccgccctg
    19141 gccgacggcg gggatcccga cgactacgcg catgccctcg aacgctggct cgcgcttggc
    19201 ggcgccgatc tcgaaaccag gctcgcggcc acgctcgacc aactcggcct cgaacctgcg
    19261 ctggaccgtc cgctcggcac cctttccggc gggcaggcgg ccagggtcgc gctggcgtcc
    19321 atcctggtca gccagtacga cgtcctgctg ctggacgagc cgaccaacaa cctggacgcg
    19381 gccgggctca aagccatgac tggtttcgtc accggccagc gctcccccgt cttgatcgcc
    19441 tcgcacgatc gcgcgttcct cgacgacgtg gccacgtcgg tggtcgaact cgacctgcat
    19501 cagcag
//
LOCUS       DAOPRQ010000051        18660 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_2514_length_18660_cov_11.428272, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000051 DAOPRQ010000000
VERSION     DAOPRQ010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 18660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18660
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2514_length_18660_cov_11.428272"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..991
                     /gene="ispG"
                     /locus_tag="PKJ61_07915"
                     /gene_synonym="gcpE"
     CDS             <1..991
                     /gene="ispG"
                     /locus_tag="PKJ61_07915"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HOC13790.1"
                     /translation="LQQIAELTAAGCDIVRVAVPSQDDADVLHIIAKKSQIPVIADIH
                     FQPKYVFAAIDAGCAAVRVNPGNIRAFDDQVKEIARAASEAGVSIRIGVNAGSLDKRL
                     LAKYGAPTPEALVESALWEASLFEEHGFRDFKISVKHHDPVVMVRAYEQLSEACDYPL
                     HLGVTEAGPAFQGTIKSSVAFGALLAKGIGDTIRVSLSAPPAEEVKVGIKILESLNLR
                     PRHLEIVSCPSCGRAQVDVYTLAEEVTEGLAGMEVPLRVAVMGCVVNGPGEAREADLG
                     VASGNGKGQIFVRGEVIKTVPEAEIVATLIEEANRLAAEMVGSDSGPVQVSVG"
     gene            994..1821
                     /locus_tag="PKJ61_07920"
     CDS             994..1821
                     /locus_tag="PKJ61_07920"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015070883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOC13791.1"
                     /translation="MDARILTSADTELARRLVAEDPVTNVFVGSRLMAGVLNPGMPGL
                     IWGWPAHAPRALLHIGANLVPVATDLDPIPAFVEAAGRRRTCQSIVGESWLAIPLWQA
                     LSDRWGRAYSAVREVRRSQPVMAAADTPLVAPDPRVRPITHADFESYFAAAVAMYTEE
                     VGTDPLAGGDSSYRSYCRWLVDQHRAFGIVEDGKVVFKSDIGAAWGSAAQIQGVWLDP
                     ALRGRGLSEPAMAAVTEYVLADYPVACLYVNDFNTRAIRCYQRVGYEQVGEFATVLY"
     gene            complement(1924..2403)
                     /locus_tag="PKJ61_07925"
     CDS             complement(1924..2403)
                     /locus_tag="PKJ61_07925"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HOC13792.1"
                     /translation="MTQTPIPDGAQRVIARLAELGHPHEVVMLPVTGRTAADAATALG
                     VELGQIAKSIVFRRRSDDAAVLVVTSGDLRVDEKKISALVGKVGRADADFVKASTGFS
                     IGGVSPVAHSQPPVMLIDRELFRFEQIWAAAGHPYAVFPLSPAELVELTGAPAAEIA"
     gene            2513..3253
                     /locus_tag="PKJ61_07930"
     CDS             2513..3253
                     /locus_tag="PKJ61_07930"
                     /inference="COORDINATES: protein motif:HMM:NF016818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-interacting protein"
                     /protein_id="HOC13793.1"
                     /translation="MTKARFETVPVESALRPVSLVAREWLTTGYLRLRLSGPQLIGFN
                     SPGADDHVRIFIAPPGVPAPTTPEAWRELDSREYTPLNFGADWVEFDFVVHGDGPGST
                     WAVTAPIGAAAAIGGPRRSNAIVGTPDAWFLAGDETAIPAISRFLRERDPATTARVLV
                     EVDPANELAPIPTDPRVELTLLVRGTDSLAEALAALQPSDRPNGALLGFVAAEAAIVP
                     VARELLLVRWGLAPESTITKGYWRIDPK"
     gene            3506..5278
                     /locus_tag="PKJ61_07935"
     CDS             3506..5278
                     /locus_tag="PKJ61_07935"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOC13794.1"
                     /translation="MSQLFVRTLREDPADAEVPSHRWLVRAGYIRRAAPGIYTWLPLG
                     LRVLHQVEKIVREEMDAMGAQEVLFPALLPREPYEATNRWTEYGPNLFRLKDRKGGDY
                     LLGPTHEEMFTLLVKDLYSSYKDLPLSIYQVQTKYRDEARPRAGLLRGREFVMKDSYS
                     FDVDDSGLAASYDAHRGAYIRMFDRLGLQYVIVKATSGAMGGSASEEFLAISPTGEDT
                     FVRSPGGYAANVEAVSITPPAELDYSGTGPARIVDTPGTGTIDELVAAANAKFPRDDR
                     AWTGADTLKNVVFKLTTPDGKSEVLIVGVPGDRDIDLKRLEAAIAPAEATPLTDDDFA
                     ANPLLVKGYIGPITLVGDQVTAHLGEEAIAKVRYLVDPRVVTGTAWITGANQVDTHMV
                     DVVAGRDFTPDGVLDVAEIRDGDPAPDGSGPLSLARGIEMGHIFQLGRKYAEALGLKV
                     LDVNGKLVTVTMGSYGVGVSRAVAAVAEATCDERGLAWPRALAPFDVQVLATGKGEEI
                     FAAAEQLANDLDAAGIRVLLDDRKASPGVKFTDAEVMGMPTSVVVGRGLADGLVEIRD
                     RATGERTEVPLAEALPHLIALVHG"
     gene            5375..5881
                     /gene="rimP"
                     /locus_tag="PKJ61_07940"
     CDS             5375..5881
                     /gene="rimP"
                     /locus_tag="PKJ61_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013161334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HOC13795.1"
                     /translation="MNTADIEALVAPVAAGHALEVDRLEVLSAGKRSVVRVFLDGDGA
                     DGRGPSLDDIAAATRDISTALDEADVTKGRPYTLEVSSRGVTRPLTEAKHFRRNVGRL
                     VAFSLAAEEVTGRVLAADDAEVDIEVDGARRTVPLADVRRAVVQVELNRPVPDDEVAD
                     DSVEDEES"
     gene            5883..6851
                     /gene="nusA"
                     /locus_tag="PKJ61_07945"
     CDS             5883..6851
                     /gene="nusA"
                     /locus_tag="PKJ61_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016455562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HOC13796.1"
                     /translation="MDVDLSALKALERDKEISMELLLDALSEALLNAYAKTPGAIPGA
                     RVQIDRKSGKVAVLAPELDEDGNRIGEYDHTPEGFGRVAAATARQVIFQRLREAEDEQ
                     KYGHFAGVEGDIVLGIVQQDRDSRTVRVDLGSIEAIMPLAEQVPGESYAHGTRLRVYV
                     VSVRRELRGPQVVVSRTHPNLVAKLFKLEVPEIESGVVEIKAIAREAGHRTKIAVISH
                     NPDVSAKGACIGPMGQRVRAVMHELNEEKIDIIDYSDDPANFVAQALSPAKVNSVTVT
                     DATARAARVIVPDYQLSLAIGREGQNARLAARLTGWRIDIRSDTAE"
     gene            complement(6868..7389)
                     /locus_tag="PKJ61_07950"
     CDS             complement(6868..7389)
                     /locus_tag="PKJ61_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13797.1"
                     /translation="MISKAKIGLGVGAFVIALGAAVGVGALAASLASGDDSAATPSGG
                     SSQAQGQGRQGGSGRGGFDTATIAKTLATKLGVDEAKVETALKEVMTASRPSGDASGR
                     PSGQPSGEPSGQPGNGGGPGGSQMIESMAKSLAEKLDLDEAKVLAALQETMSAGRPAH
                     DQPSGQPSSEPTK"
     gene            7508..7768
                     /locus_tag="PKJ61_07955"
     CDS             7508..7768
                     /locus_tag="PKJ61_07955"
                     /inference="COORDINATES: protein motif:HMM:NF016204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HOC13798.1"
                     /translation="MSQPVRTCLGCRQRAHKAELVRLVWDSTTGAVVIDAGQVLPGRG
                     GYVHPTCGDALAKRRQLGRALRRPVDAEQAAALLAALTDSTD"
     gene            8132..11032
                     /gene="infB"
                     /locus_tag="PKJ61_07960"
     CDS             8132..11032
                     /gene="infB"
                     /locus_tag="PKJ61_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002516995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOC13799.1"
                     /translation="MAKVRVYELAKELGLESKELLQTLNDMGEFVRSASSTIEAPVVR
                     RLKEKAAAAQPAPAQSAPAPAPARPAASTPPARTAPAETAPASVAPAAPAPAVPAPTA
                     RPAAPTPGPAAAKPAAAPVAETAPALAPTAPAAPSAAAAAAPAPAAPAEPTPAASSAT
                     PRPGAMPGPRPAAPRPAPRPGGTGGLPGAGARKPGAPRPGNNPFASRQGMGVQRPRPE
                     GAAPGRPGERPYSDRPGGPRPAGDRMPRPGGGVAGMPRPNPAMMPKTASGNLGGPGRT
                     GVGRPGGAGAGGRGRPGGAPGRSGPGGAPGTGGPPGGGGRGGRGGRGGSGTQGAFGRA
                     GGPARRGRKSKKQRRQEFDQMEAPTIGGVRVRQGDGQTVRLRRGASLTDLAEKIGVEP
                     ASLVQVLFHLGEMVNATQSVADDTLQVLGAELNYNIEVVSPEDEDRELLESFDIEFGE
                     NEGGEEDLAPRPPAVTVMGHVDHGKTKLLDALRNTNVVAGEAGGITQAIGAYQVSTEV
                     DELERKITFIDTPGHEAFTAMRARGAKSTDIAVLVVAADDGVMPQTIEALNHAKAADV
                     PIVVAVNKIDKETADPVKVRGQLTEYGLVPEEYGGDTMFVDVSATARLGLDELLEAIV
                     LTADASLDLRANPDMPAQGVAIEAHLDKGRGPVATVLVHRGTLRTGDSIVAGSAYGRV
                     RALINDQGETVDSAPPSMPVQVLGLTSVPGAGDNFLVVEDDRMARQIAEKREARQRAA
                     MLAKTTRRKTLDQLFEQLAKGEAQELRLILKGDSAGAVEALEDALLQIDVGDEVDLRV
                     IDRGVGAITETNVSLAAASDAVIIGFNVRPQGKATQLADAEGVDIRYYSVIYAAIDEV
                     EAALKGMLKPIYEEKVRGQAQIREIFRSSKFGVIAGCMVIDGSIRRNAKARLLRDGVV
                     VAETSIASLRREKDDATEVREGFECGMTLANYSDIRIDDIIETFEMVEKPRD"
     gene            11102..11539
                     /gene="rbfA"
                     /locus_tag="PKJ61_07965"
     CDS             11102..11539
                     /gene="rbfA"
                     /locus_tag="PKJ61_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOC13800.1"
                     /translation="MSNPRFAKVAEQIKFIIAEMLQRRIKDPRLGFVTVTDVRITGDG
                     REATVFYTVLGDAEARAESAVALASATGLLRSTVGKQLGMKFTPTLAFVLDGVEEEAA
                     HLEDVLAKARAQDAAVAAQAAGASYAGEADPYRHPDEEDDELA"
     gene            11544..12497
                     /gene="truB"
                     /locus_tag="PKJ61_07970"
     CDS             11544..12497
                     /gene="truB"
                     /locus_tag="PKJ61_07970"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOC13801.1"
                     /translation="MAPADGGLVVVDKPSGWTSHQVVARCRRLLGTRKVGHAGTLDPM
                     ATGLLLVGFGRATRLLGYLAKAPKTYVATIRLGQSTITDDAEGEVVAAVGAAGLTEGD
                     VRAALPAFTGVISQVPSAVSAIKVDGKRAYARVRAGEDVQLAAREVTVSRFEVLAVRR
                     ATSAERTVSRHDGEEPSISVVDSGYPVAVLDVDVVVDCSSGTYIRALARDLGERLGVG
                     GHLTALRRTRIGAAEQADIRLDAAGLAGDARPEAIGMVQLARDFFPVAEVTAEQASDI
                     GFGRSLRLAVPADPTAMVYGDQLLALYRPDGEVARPVAVLA"
     gene            12537..13472
                     /locus_tag="PKJ61_07975"
     CDS             12537..13472
                     /locus_tag="PKJ61_07975"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002518460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HOC13802.1"
                     /translation="MGSATVVIGNFDGVHPGHQEVLAEAKRRRPSLPLFVVTFWPHPA
                     SVLRPEVGPMLLTSLEARIDLLSAAGADRVEVIDFTPEFASWSPSDFVERVLFPLDPA
                     LVVVGENFRFGNRAVGSVATLRELAAGRFEVEGLPLVRFGDEETCSTRIRQALAEGDV
                     AHAAEHLGRPFCFRGVVAHGDRRGRTLGFPTANLPVPANLACPEDGVYAGWVTRRDGL
                     DAHGESLAAGAAPECWPAAISVGTNPTFDGVSRRVESYVLDRTDLELYGAPISVEFIA
                     RIRGQVRFNNIDALVAQMTDDVDRCRELLRRAEHN"
     gene            13512..13916
                     /locus_tag="PKJ61_07980"
     CDS             13512..13916
                     /locus_tag="PKJ61_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13803.1"
                     /translation="MFGHRVWRPAAAGLDLRADDLAELTELSHLIVAEYAVSSPTVLA
                     ATIQRLLQRRVPILRIEHSDSADDGYHLRFADGTALLVRGRHPGDVGWTAANALLNRV
                     QLVGYACLAEEVTLDLVGGRRHVRLVAIGMDR"
     gene            complement(13917..14408)
                     /locus_tag="PKJ61_07985"
     CDS             complement(13917..14408)
                     /locus_tag="PKJ61_07985"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOC13804.1"
                     /translation="MTDEPITVRLCTAEDLPALHAREAHPSARIAQRHFDLAAAGDYY
                     YAVAFRGDVPLGTCAMDAHEHNPLCPELMNLWVYPDARRQGAAKAMTRFLEGKAAERG
                     LVEVFLRVDPNNEAAIPMYIGLDYTPTGDHAVTSYETVAADGAVTTTEKTDAIYRKSL
                     LTR"
     gene            14564..15367
                     /locus_tag="PKJ61_07990"
     CDS             14564..15367
                     /locus_tag="PKJ61_07990"
                     /inference="COORDINATES: protein motif:HMM:NF018822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LppX_LprAFG lipoprotein"
                     /protein_id="HOC13805.1"
                     /translation="MASSQRVLGLALTAILLSGCSAPAPATVTQGTDAPASSAAAPQT
                     SVAAPAGDSGTDEFVSKLSTAMAGVKTYSFDIDMETEVSGQAAVIKTKGVLDQTDAAN
                     KKMQMDANIAGMAMKMLQVDGELYVQMEVLGDKWMLVPEDQRSNYMEATKTGDMAKSF
                     EQSKDLIKSLETVGDETVDGVATKHYLVTYDGSALSGLTGGTGSVEGTLEYHMWVDAD
                     SLIRKVSMEAAASEDSEVVPFKMSGTMGDYNEPVTIKAPSKDDITEMPS"
     gene            complement(15548..16978)
                     /gene="pntB"
                     /locus_tag="PKJ61_07995"
     CDS             complement(15548..16978)
                     /gene="pntB"
                     /locus_tag="PKJ61_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016668421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - NAD(P)+ transhydrogenase
                     (AB-specific) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HOC13806.1"
                     /translation="MLLQFVQGLYIVAGVLFILSLAGLSKQTTARLGNVAGMIGMGLA
                     LVATVIHSLNISATEDDRLPVWVTLVLIVAVLGIGAFLGTPIARKVEMTQMPELIAAF
                     HSFVGMAAVLVGFNSFAVPGETGGGAVHMVEVALGVLIGAYTFTGSVVAYLKLSAKMK
                     SQPLMLPGRNLINLTGFVGFLALMVWFVTVGEHNALLGWVLIIAIALIGFALGYHMVA
                     AIGGGDMPVVVSMLNSYSGWAAAAAGFMLNNNLLIITGSLVGSSGAILSYIMCRAMNR
                     SFISVILGGFGSEGGQVSSGAEETGEHREIDAATTAEMLWNAKSVIITPGYGMAVAKA
                     QYPVADLTAKLRAHGVKVRFGVHPVAGRLPGHMNVLLAEAHVPYDIVLEMDEINDDFA
                     ETDVVLVIGANDTVNPAALEDPNSPIAGMPVLEVWKAENVIVFKRSMATGYAGVQNPL
                     FFKENTHMLFGDAKERVDDILKALGK"
     gene            complement(16981..18537)
                     /locus_tag="PKJ61_08000"
     CDS             complement(16981..18537)
                     /locus_tag="PKJ61_08000"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013161990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HOC13807.1"
                     /translation="MQIGIPLETLHDETRVAATPKTVAQLIKLGHEVIVEAGAGAKSA
                     YPDAEYAAAGATIVGRAKAWSAPVVAKINAPTDAEIGQLPEGGMLISLIAPARNPELL
                     AKLNQRKLTVLAMDAVPRISRAQALDVLSSMANIAGYRAVVEAANEFGSFFTGQVTAA
                     GKVPPAKVLVAGAGVAGLAAIGTAKSLGAIVRATDTRPEVGEQVASMGGEFVSPRAIT
                     QQVSADGYAKEMGDDYKAAAAELYAEQAKDVDIVITTALIPGKPAPRLLTADMVASMR
                     PGSVIVDMAAANGGNVEGSRPDEVVTTTNGVKIIGYTDLAGRLPTQASQLYGTNVVNL
                     FKLLTPAKDGEVTVDLGDVVVRGMTVTDNGELLWPPPPVQVSAAAVPAVVEPAEDPAV
                     RTAREARAAAGKARNRIVMSAIAAALVILAVTFSPASFVGAFTVFALAVVVGFYVVSG
                     VSHSLHTPLMAQTNAISGIILVGALLQLGSTNIAVLVMSFIAATIASINIFGGFLVSY
                     RMLSMFKKEA"
BASE COUNT         2965 a         6248 c         6512 g         2935 t
ORIGIN      
        1 cctgcagcag atcgccgagc tgaccgcggc cggctgcgac atcgtccggg tcgccgttcc
       61 cagccaggac gacgccgacg tgctgcacat catcgccaag aagagccaga tcccggtcat
      121 cgccgacatc catttccagc cgaagtacgt gttcgccgcg atcgacgccg gctgcgcagc
      181 cgtccgggtg aacccgggga acatccgggc cttcgacgac caggtcaagg agatcgcccg
      241 cgcggccagc gaggccgggg tctcgatccg cataggtgtc aacgccggtt cgctggacaa
      301 gcggctgctc gccaagtacg gcgcgccgac cccggaggcg ctggtcgagt ccgcgctctg
      361 ggaggccagc cttttcgagg agcacggctt ccgcgacttc aagatttcgg tgaagcacca
      421 cgacccggtg gtcatggtgc gtgcctacga gcaactatcc gaggcgtgcg actacccgct
      481 gcacctgggc gtgaccgagg ccggacccgc tttccagggg acgatcaagt cgtcggtggc
      541 cttcggcgca ctgctggcga agggcattgg cgacaccatc cgcgtctcgc tgtccgcgcc
      601 gccggccgaa gaggtgaagg tcggcatcaa gatcctggag tcgctcaacc tgcgtccgcg
      661 gcacctggag atcgtctcgt gcccttcctg cggacgcgcc caggtggatg tctacaccct
      721 cgccgaggag gtcaccgagg ggctcgccgg catggaggtg ccgctgcggg tcgcggtgat
      781 gggttgcgtg gtcaacggcc ccggcgaggc gcgggaagcc gatctcggcg tcgcgtccgg
      841 caacggcaag ggccagatct tcgtccgcgg cgaggtgatc aagaccgtgc ctgaggctga
      901 gatcgtggcc acgttgatcg aggaggcgaa ccggctggcc gcggagatgg tgggcagcga
      961 ctccggcccc gtccaggtct cggtcggata gccatggacg cccggatcct caccagcgca
     1021 gacactgagc tcgcccgccg gctggtggcg gaggacccgg tcacgaacgt cttcgttggc
     1081 tccaggttga tggccggggt gctgaatcct ggtatgccgg gcctgatctg gggctggccg
     1141 gcccacgcgc cacgtgcctt gctgcacatc ggggccaacc tggtcccggt ggctaccgac
     1201 ctggacccga taccggcgtt cgtcgaagca gccggacgcc gtcgcacctg ccagtcgatc
     1261 gtcggcgagt cctggctggc gattccgctg tggcaggcgc tgagcgaccg gtggggacgc
     1321 gcctacagtg cggtacgcga agttcggcgc agccaacctg taatggctgc ggcggatacg
     1381 ccgctggtgg cgcccgaccc gagagtgcgg ccaataacgc atgccgattt cgagtcgtac
     1441 ttcgccgccg cggtggcgat gtacaccgag gaggtcggca ctgacccact ggccggcggt
     1501 gacagcagct atcgcagcta ctgtcgctgg ctggtcgacc agcaccgcgc gttcggcatc
     1561 gtcgaggacg gcaaggtggt cttcaagagc gacatcgggg ccgcctgggg cagtgccgcg
     1621 cagatccagg gcgtctggct cgacccggca ctacgcggac gcgggctgtc cgagccggcg
     1681 atggcggcgg tgaccgagta cgtcctcgcc gactacccgg tggcgtgcct ctacgtgaac
     1741 gacttcaaca cccgcgcgat ccggtgctac cagcgggtcg gctacgaaca ggttggcgag
     1801 ttcgccaccg ttctgtactg agcccggacg gaatctcccg taatcccggc cgctctcgtg
     1861 gtcccggccg ctctcgtcat cccgggcttg acccgggatc tccggccgcg acccgacgat
     1921 gcgtcaggcg atctccgccg cgggtgcacc ggtgagttcg accagctcgg caggcgatag
     1981 cggaaacacc gcatatggat gtcctgccgc cgcccagatc tgttcgaagc gaaacaactc
     2041 gcggtcgatc agcatcaccg gcggctggct gtgtgccacg ggagagactc cgccgatcga
     2101 aaatccggtg gacgccttga cgaagtcggc gtcggcgcgg ccgaccttgc cgaccagcgc
     2161 agagatcttc ttctcatcga cccgcaggtc gccggaggtg accaccagta ccgcggcgtc
     2221 gtcggatcgt cggcggaaca cgatgctctt cgcaatctgc ccgagctcta cgcccagcgc
     2281 ggttgcggcg tcggctgcag tacggccggt taccgggagc attaccacct cgtgcggatg
     2341 gccgagttca gccagcctgg cgatcacgcg ctgcgcaccg tcaggaatcg gagtctgcgt
     2401 cacgagtgca ttttgccagc cgctgggtat gcgtccgcca gtgcttgtct cgggttggca
     2461 gactccagga gtgatgctta ggttagccaa acttaactct ggcgggaggg tgatgacgaa
     2521 ggctcggttc gagacggttc cagtggagtc cgcgctgcgg ccggtttcgc tggttgctcg
     2581 ggagtggctc accacgggct acctgcggct ccggctgtcc ggcccgcaac tgatcggctt
     2641 caactcccca ggtgccgacg atcacgtgcg tatcttcatc gctccgcctg gcgtgcccgc
     2701 tcccaccacg ccggaggctt ggcgggagtt ggacagccgg gagtacaccc cgctgaactt
     2761 cggtgctgac tgggtggagt tcgacttcgt ggtgcacgga gacggtcccg gctcgacctg
     2821 ggcggtgacg gccccgatcg gtgccgcggc cgcgatcggg ggcccgcgtc gcagcaacgc
     2881 aatcgtcggc accccggacg catggttcct tgccggtgat gagaccgcga tcccggcgat
     2941 cagccggttc ctccgggaac gcgaccccgc gacgaccgcc cgcgtcctgg tcgaggtcga
     3001 cccggcgaac gaactggcgc cgatccccac cgaccctcgg gtcgagctca ctctgctggt
     3061 gcgcggcacc gactccctgg ccgaagcact tgccgcgctg cagccgtccg accgtcccaa
     3121 cggagcgctg ctcggcttcg ttgccgccga ggccgcaatc gtgccggtcg cccgcgaact
     3181 cctgttggtt cgctgggggc tggcccccga aagcacgatc accaagggct actggcgtat
     3241 cgaccccaag tagtgcgcgg tcgtgaggtt cgcgccggcg aaactgacgt tcgccggtga
     3301 atgttccctg gcgaacgtcc ggatcgctgg ctagacgacg accttggagg gcggagccgc
     3361 gcaatgggga ggagtcttga cgagcggtga ggaacgaacc gcgaggagaa gaacggcccg
     3421 ggtgggtgct ctgacagcgg acggcggact gcgcgcgggc agcgatggcc tccgcgtcca
     3481 ataggatcac cgggtgatca cgcgactgtc ccagctgttc gtccgtaccc tccgcgagga
     3541 ccccgctgac gccgaggtgc ccagtcaccg ctggctcgtg cgcgcgggct acatccgccg
     3601 cgccgcaccg ggcatctaca cctggctgcc gctgggcctg cgggttctgc accaagtgga
     3661 gaagatcgtc cgcgaagaga tggacgcgat gggcgcccag gaggtgctct tccccgcgct
     3721 gctgccccgg gagccctacg aagccaccaa ccgctggacg gagtacggtc ccaacctctt
     3781 ccggctgaag gaccgcaagg gcggcgacta cctgctcggc ccgacccacg aggaaatgtt
     3841 caccctcctg gtgaaggatc tctactcctc gtacaaggac ctgccgctct cgatctacca
     3901 ggtccagacc aagtaccgcg acgaggcccg gccgcgcgcc ggtctgctgc gcggccgcga
     3961 gttcgtgatg aaggattcgt actccttcga cgtcgacgat tcgggcctgg ccgcgtccta
     4021 cgacgcgcat cgtggtgcct acatccgcat gttcgaccgg ctcggcctgc agtacgtgat
     4081 cgtgaaggcc acgtccggtg cgatgggcgg ttcggcgtcc gaggagttcc tggcgatctc
     4141 acccactggt gaggacactt tcgtccgctc gcccggtggc tacgccgcca acgtcgaggc
     4201 cgtcagcatc accccgcccg ccgaactcga ctactccggt accggcccgg cgcgcatcgt
     4261 tgacaccccg ggcacgggca ccatcgacga actggtggcc gcggccaacg ccaagttccc
     4321 ccgcgacgat cgtgcctgga cgggggccga caccctcaag aacgtcgtgt tcaagctgac
     4381 cacaccggac ggcaagagcg aggtgctgat cgtcggcgtg cccggcgacc gggacatcga
     4441 cctcaagcgg ctcgaagccg cgatcgcccc ggcagaggcg actccgctca ccgacgacga
     4501 cttcgccgcc aacccgctgc tggtcaaggg ctacatcggc ccgatcaccc tggtcggtga
     4561 ccaggtaacc gcgcaccttg gcgaggaggc gatcgcgaag gtgcgctacc tggtcgatcc
     4621 gcgggtggtc accggcaccg cctggatcac cggcgccaac caggtcgaca cccacatggt
     4681 cgacgtggtc gccgggcgcg acttcacccc ggacggggtg ctggacgtgg ccgagatccg
     4741 cgacggcgac ccggctccgg acggctccgg gccgctgagc ctggcccgtg gcatcgagat
     4801 gggccacatc ttccagctgg gccgcaagta cgccgaggca ctcggcctca aggtgctgga
     4861 cgtcaacggc aagctggtca ccgtgaccat gggctcgtac ggcgtgggcg tgtcccgggc
     4921 ggtcgccgcc gtcgccgaag cgacctgcga cgagcgcggc ctggcctggc cgcgggcgct
     4981 ggcaccgttc gacgtgcagg tgctggccac cggcaagggc gaggagatct tcgccgcagc
     5041 cgagcagttg gccaacgacc tggacgccgc cggcatccgc gtcctgttgg acgaccgcaa
     5101 ggcatcgccg ggggtcaagt tcaccgacgc cgaggtgatg ggcatgccca cctcggtggt
     5161 ggtcggacgt ggactggccg acggtctggt cgagattcgt gaccgggcca ccggggagcg
     5221 caccgaggtg ccgctggccg aggccctgcc gcacctgatc gcgctcgtcc acggctgagc
     5281 ccggatccgg gctgggtcgc ttggcccacg cactagactt gggcggacgc ctgcgtcccc
     5341 tacaaccgca tcacacaatc ggatcgaggt agtcatgaac accgctgaca tcgaggcgct
     5401 ggtggctcca gtcgccgccg ggcacgcgct cgaggtggac cgcttggaag tgttgagcgc
     5461 tggaaagcga tcggtggtga gggtcttcct cgacggtgat ggtgccgacg ggcgcggccc
     5521 gtccctggac gacatcgccg cggccacacg tgacatctcg accgcgctgg acgaggccga
     5581 cgtgaccaaa ggtcggccct acacccttga agtctcctcg cgcggcgtga cccgtccgct
     5641 caccgaggcc aagcacttcc gccgcaatgt gggacgcctg gtcgcgttct cgctggctgc
     5701 cgaagaggtg accggacgcg tcctcgcggc cgacgatgcc gaagtcgaca tcgaagtgga
     5761 cggagcccgg cggacagtgc cgctcgccga tgtgcgccgc gccgtggtgc aggtggaact
     5821 caaccggccg gtgccggacg acgaggtagc ggacgattcc gtagaggacg aggagagctg
     5881 agatggacgt cgacctgagc gcgctgaagg cgctggaacg cgacaaggaa atcagcatgg
     5941 agctgctgct ggatgcactc agcgaagctc tgctgaacgc ctacgccaag accccgggag
     6001 cgattccggg tgcgcgggtg cagatcgacc gcaagagcgg caaggtggcg gtcctggcgc
     6061 ctgagctgga cgaagacggc aaccggatcg gggagtacga ccacaccccg gagggtttcg
     6121 gccgggtggc cgccgcgacc gcacggcagg tgatcttcca gcgactgcgg gaggccgagg
     6181 acgaacagaa gtacggccat tttgctgggg tcgagggcga catcgtcctt ggcatcgtgc
     6241 agcaggaccg cgactcgcgc accgtccggg tggacctcgg cagcattgag gcgatcatgc
     6301 cgctggccga gcaggtgccg ggggagtcgt acgcccacgg gacccggctg cgggtctacg
     6361 tggtcagcgt ccgccgggag ctgcgcgggc cgcaggtcgt ggtgtcacgt acccacccca
     6421 acctggtggc gaagctgttc aagctggaag tgcccgagat cgagtccggg gtggtggaga
     6481 tcaaggcgat cgcccgcgag gccgggcacc gcacgaagat cgcggtgatc agccacaacc
     6541 cggacgtttc ggccaagggc gcctgcatcg ggccgatggg gcagcgggtg cgcgcggtga
     6601 tgcacgagct gaacgaggag aagatcgaca tcatcgacta ctccgacgat ccggcgaact
     6661 tcgtcgcgca ggcgctcagc ccggccaagg tgaactcggt cacggtgacg gacgcgaccg
     6721 cgcgcgcggc ccgggtgatc gtgccggact accagctctc gctggcgatc ggtcgcgagg
     6781 gccagaacgc ccgcttggcc gcccgcctca ccggctggcg gatcgacatt cgctcggaca
     6841 ccgccgaata gtcgctgccg gccgaaccta cttcgtaggc tcgctggacg gctgcccgct
     6901 gggctggtcg tgtgccggac ggccggccga catcgtctcc tgcagcgcgg ccagcacctt
     6961 ggcctcgtcc aggtcgagct tctcggccag gctcttggcc atcgactcga tcatctggct
     7021 gccacccggg ccgccgccgt tgcccggctg accggacggt tcacccgagg gctggccgga
     7081 cggccgcccc gacgcatcac cggacggccg gctggccgtc atcacctctt tcagcgcggt
     7141 ctcgaccttg gcttcgtcga cgcccagctt ggtcgccagg gtcttggcga tggtcgcggt
     7201 gtcgaagcca ccccgcccgg aaccgccctg gcggccctga ccctgggcct gactggagcc
     7261 accggacggg gtcgccgcgg agtcgtcgcc gctggccagg ctcgcggcca gggcacccac
     7321 accgaccgcc gcgccgaggg cgattacgaa ggcgcccact cccaaaccga tcttggcctt
     7381 gctgatcatt gctctcctcg aagtttgtcc ggaatccaga caagccggtg ggcctgtgct
     7441 ggcagtcagc cgaaccttgg tgtttcctgt ggaaagcgtg gtcgtgggga tggatagagt
     7501 cggagccgtg agccaacccg tccgcacgtg cctcggctgt cgccagcgcg cccacaaggc
     7561 ggagttggta cgcctggtct gggactcgac caccggcgcg gtggtgatcg atgctggtca
     7621 ggtgctgccc ggacgcggcg gctatgtgca ccccacgtgc ggggacgcgc tagccaagcg
     7681 acggcaactc ggccgcgcgc tgcggcgtcc ggtcgacgct gaacaggcgg ccgcactgct
     7741 ggctgcgctc actgactcca ccgactgatc cgcccaggcg gcccaggcca cgaggtcgcg
     7801 ttcccggcca ccccacccgc caaaccaact tcgctcccga aagcgacccg cgctcccgat
     7861 aattcgggag caaacgtccc attcgggagc gaagttgcct gggggagggt tggctgggct
     7921 gttgggtcag gccgccgccg ggggcggcgt gggagccgat tgagtcttat tgcgctcggc
     7981 gcgataagct tgacgccgcg cgtccgccaa gttcagcggc ggacacaacc agaaagtggg
     8041 ctaacgctca tgaccactcg atgagtacct cgaaatgagc actgaacaga actaactggt
     8101 cgacgcgccc gcgtggacct gaaggagagt agtggctaag gtccgcgtct acgagctcgc
     8161 caaggagctc ggactcgaaa gcaaggaact tctgcaaacc ctcaatgaca tgggggaatt
     8221 cgtccggtcg gcctcctcga cgattgaggc cccggtggtt cggcggttga aggaaaaggc
     8281 cgccgcagcc caaccagcac ccgcccagtc ggcgcccgcg cccgctccgg cgcgcccggc
     8341 cgccagcacc ccgccggcca ggaccgcacc ggccgagact gccccggcca gcgtcgcccc
     8401 tgcggcgccc gccccggccg tcccggcgcc caccgcccgt ccggctgccc cgacccctgg
     8461 cccagcggcc gccaagcccg ccgcagcgcc ggtggccgag accgcgccgg ctctcgcacc
     8521 gacggcacct gctgcgccca gcgcggccgc ggctgccgct ccggcgccgg ccgccccggc
     8581 cgagccgacc ccggcggctt cgtccgcgac gccacgtcct ggtgccatgc ccggtccgcg
     8641 tccggctgcc ccgcggcccg cgccgcgtcc gggtggcacc ggcggcctgc ctggcgccgg
     8701 cgcccgcaag cccggtgcac cgcgtccggg caacaatccg ttcgcttccc gacagggcat
     8761 gggcgtgcag cgtccgcggc ctgagggtgc cgcaccgggt cgtccgggcg agcgtcccta
     8821 cagtgatcgt cccggcgggc cgcgtccggc cggtgatcgc atgccgcgtc ccggtggcgg
     8881 tgtcgcgggc atgcctcgac ccaatcccgc gatgatgccg aagactgctt cgggcaacct
     8941 cggtggcccc ggccgtaccg gtgtcggtcg cccgggtggt gccggcgctg gcggacgtgg
     9001 ccgtcccggc ggtgctcccg gtcgcagcgg ccccggtggc gcgcccggta ccggtggtcc
     9061 tcccggtggt ggtggccgtg gcggtcgtgg tggccgtggc ggctcgggca cccagggtgc
     9121 gttcggtcgc gccggtggtc ccgcgcgccg tggccggaag tcgaagaagc agcgcaggca
     9181 ggaattcgac cagatggagg cgccgacgat tggtggcgtg cgcgtccgtc agggcgacgg
     9241 ccagaccgtc cggctgcgcc gcggcgcttc gctgaccgac ctcgccgaga agattggtgt
     9301 cgagccggcg tccctggtgc aggtgttgtt ccacctgggc gagatggtca atgccaccca
     9361 gtcggtggcc gacgacaccc tgcaggtgct gggtgcggag ctcaactaca acatcgaggt
     9421 cgtctcgccc gaggacgagg accgcgaact gctggagagc ttcgacatcg agttcggtga
     9481 gaacgagggt ggcgaggagg acctcgcccc gcgtccgccc gccgtcaccg tcatgggtca
     9541 cgtcgaccac ggcaagacga agctgctgga cgcgctgcgc aacaccaacg tggtcgccgg
     9601 cgaggccggt ggcatcaccc aggcgatcgg cgcctaccag gtgtccaccg aagtggacga
     9661 gttggagcgc aagatcacct tcatcgacac cccgggccac gaggcgttca ccgccatgcg
     9721 tgcccgtggt gcgaagtcga ccgacatcgc ggtgctggtg gtggccgccg acgacggtgt
     9781 gatgccgcag acgatcgagg ctctgaacca cgccaaggcg gccgatgtgc cgatcgtggt
     9841 cgcggtgaac aagatcgaca aggagaccgc tgatccggtc aaggtgcgcg gtcagctcac
     9901 cgagtacggc ctggtgccgg aggagtacgg cggcgacacc atgttcgtcg acgtgtccgc
     9961 caccgcccgc ctcggtctgg acgagctgct ggaggccatc gtgctgacgg cggacgcgtc
    10021 cctggacctg cgggccaacc cggacatgcc cgcccagggt gtcgccatcg aggcgcacct
    10081 ggacaagggc cgcggtcccg tggccaccgt cctggtgcac cgcggcacgc tgcgcaccgg
    10141 cgattcgatc gtggccggtt cggcttacgg ccgcgtccgc gccctgatca acgaccaggg
    10201 tgagaccgtc gattccgcgc cgccgtccat gccggtgcag gtgctcggcc tgacctcggt
    10261 gcccggagct ggtgacaact tcctggtcgt cgaggacgac cggatggctc gccagatcgc
    10321 cgagaagcgg gaagcccggc agcgggcggc catgctggcc aagaccaccc ggcgcaagac
    10381 gctcgaccag ctgttcgagc agctggccaa gggcgaggcc caggagctgc ggctcatcct
    10441 caagggcgat tcggccggtg cggttgaggc gctggaggac gcgctgctgc agatcgacgt
    10501 cggcgacgag gtcgacctgc gggttatcga ccgcggtgtc ggtgcgatca ccgagaccaa
    10561 cgtttcgctg gctgcagcct ccgacgcggt gatcatcggc ttcaacgtac ggccgcaggg
    10621 caaggccact cagctggccg atgccgaggg cgtcgacatt cgctactact cggtgatcta
    10681 cgccgcgatc gacgaggttg aagctgccct gaagggcatg ctgaagccga tctacgagga
    10741 gaaggtgcgg ggtcaggcgc agatccggga gatcttccgt tcgtccaagt tcggtgtcat
    10801 cgccggctgt atggtcatcg acggttcgat ccgccgcaac gccaaggccc ggttgctgcg
    10861 cgacggggtg gtggtcgccg agacctcgat cgcgtccctg cgccgggaga aggacgacgc
    10921 caccgaggtc cgcgagggct tcgagtgcgg catgacgctc gccaactact ccgacattcg
    10981 gatcgatgac atcatcgaga ccttcgagat ggtcgagaag ccgcgcgact gagcagtccc
    11041 tgccgaaccc ggccacccct tctggggcgg ccgggttcgg gttatccgga aaggaacatc
    11101 gatgagcaac ccccgattcg cgaaggtcgc tgagcagatc aagttcatca tcgccgagat
    11161 gctgcagcgg cggatcaagg atccgcgcct tggcttcgtc acggtcaccg acgtgcgcat
    11221 caccggcgac ggccgggagg ccactgtctt ctacaccgtg ctgggggacg cagaggcgcg
    11281 ggccgagtcg gcggtcgcgc tggcctcggc caccgggctg cttcgctcca cggtcggtaa
    11341 gcaactcggc atgaagttca ccccgacgct ggcgttcgtg ctggacggtg tcgaggagga
    11401 ggccgcccac ctggaggacg tgctcgccaa ggcccgggcg caggacgccg cagtggccgc
    11461 gcaggcagcc ggggcgagct acgcgggcga ggccgatccc taccgccacc cggacgaaga
    11521 agacgacgag cttgcctgag gcggtggctc cagcggacgg cggcctggtg gtggtcgaca
    11581 agccgtctgg ctggacttcg caccaggtgg tcgcgcgttg ccggcggctg ctcggcaccc
    11641 gcaaggtcgg gcatgccggc acgctcgacc cgatggccac cggcttgctg ctggtgggtt
    11701 tcggtcgggc cacccgcctg ctcggctatc tggccaaggc acccaagacc tacgtcgcga
    11761 ccattcggct ggggcagtcg accatcaccg acgacgccga gggcgaggtg gtcgccgcgg
    11821 tcggtgctgc cggcttgact gaaggcgacg tgcgcgcggc cttgcccgcc ttcaccggcg
    11881 tgatcagcca ggtgccgtcc gcggtttcgg cgatcaaggt ggacgggaag cgcgcctacg
    11941 cccgcgtccg agccggtgag gacgtgcagc ttgccgcccg cgaggtcacg gtgtcccgct
    12001 tcgaggtgtt ggcggtgcgc cgcgccacct cggcagagcg gacggtttcg cgccacgacg
    12061 gcgaggaacc gtccatctcg gtggtggact cggggtaccc ggtggctgtg ctcgatgtgg
    12121 atgtggtcgt ggactgttcc tcgggcacct acatccgcgc cctggctcgc gacctgggcg
    12181 agcgtctggg agttggcggg cacctgactg cgctgcgacg cacccggatc ggcgcggctg
    12241 agcaggccga catcagactg gacgcggccg gcctggccgg cgacgcgcgg cccgaggcga
    12301 tcggaatggt ccagctcgca cgggacttct ttccagtcgc cgaggtgacc gcagagcagg
    12361 ccagcgacat cggcttcggc cgtagcctgc ggctggccgt gcccgcggac ccgaccgcta
    12421 tggtgtacgg cgaccagctg ttggccctct accggcccga cggcgaggtg gctcggccgg
    12481 tcgcggtact ggcctgagtt gctaccctga ccagggcacc aaccaggaag gcaagcgtgg
    12541 gatctgcaac ggtcgtcatc ggcaacttcg acggtgtgca ccctgggcat caggaggtgc
    12601 tcgccgaggc gaagcgcagg cgtcccagcc tgccgctgtt cgtggtgacc ttttggccgc
    12661 atccggcctc agtgctgcgt ccggaggtag ggccgatgct gctcacctcg ctggaggcgc
    12721 ggatcgacct gctcagcgct gccggggcgg atcgcgtcga ggtgatcgac ttcacgcccg
    12781 agttcgcgtc gtggtctccg tccgacttcg tcgaacgcgt gctgtttccg ctcgacccgg
    12841 cactcgtggt ggtgggagag aacttccggt tcgggaaccg cgctgtcgga tcggtcgcga
    12901 cgttgcgcga gctggccgcc ggacgcttcg aggtcgaagg actgccactg gttcggttcg
    12961 gcgacgagga gacctgttcg acgcgtattc ggcaggcgct ggccgaaggg gacgtggccc
    13021 atgcggccga gcatctcggc cggccgttct gcttccgcgg ggtggtggcg cacggcgacc
    13081 ggcgcggacg gacgctcggc ttcccgaccg cgaacctccc ggttccggcc aatctggcct
    13141 gccccgagga cggggtctac gcgggttggg taacccggcg ggacggcctc gacgcgcacg
    13201 gcgaatcgct tgcggcgggc gcggcgcccg agtgctggcc ggcggccatc tcggtgggca
    13261 ccaatccaac cttcgacgga gtgtcgcgcc gggtggagtc atacgtgctc gatcgcaccg
    13321 acctggaact gtacggcgct ccgatcagcg tggagttcat cgccaggatc cgcggccagg
    13381 ttcggttcaa caacatcgac gcactggtgg cgcagatgac cgacgatgtc gaccggtgcc
    13441 gggagctgct gcgccgtgcg gagcacaact gacaccttcc ttccgtcagc ccggcgggcg
    13501 acaatggaag cgtgttcggg catcgggtct ggcgaccggc cgccgccggt ctggacctgc
    13561 gtgccgacga cctggccgaa ctgaccgagt tgagccatct gatcgtggca gagtacgccg
    13621 tgagttcacc taccgtgctc gcggccacga tccaacgact gttgcagcgc cgcgtcccga
    13681 tcctgcggat cgagcactct gactccgccg acgacggcta ccacctacgg ttcgcggacg
    13741 gcaccgcact gctggtgcgc ggccgacatc ccggtgacgt cggttggact gcggcgaatg
    13801 cgttgctcaa ccgcgtccag ttggtcggtt acgcctgcct tgccgaagag gtgacgctcg
    13861 acctggtcgg cggccggcgc cacgtccgcc tggtagcgat cgggatggat cgctgatcag
    13921 cgggtgagca gcgacttgcg atagatcgcg tccgtcttct cggtggtcgt gaccgcgcca
    13981 tcggcagcga cggtctcgta gctggtgacg gcgtgatcgc cggtgggcgt gtagtcgagc
    14041 ccgatgtaca tggggatggc ggcctcgttg ttcgggtcca cccgaaggaa cacctcgacg
    14101 agaccgcgtt cggcagcctt gccctcaagg aaccgggtca tggccttcgc cgcaccttgc
    14161 cgccgggcgt cgggatagac ccacaggttc atcagctcag gacagagcgg gttgtgctcg
    14221 tgggcgtcca tcgcgcaggt gcccagcggg acatccccgc ggaaagccac cgcgtagtag
    14281 taatcgccgg cggcggcgag atcgaagtgc cgttgcgcga tccgcgcgga cgggtgcgcc
    14341 tcgcgtgcgt gcagcgcggg caggtcctcg gcggtacaga gtctcacggt gatcggttcg
    14401 tcggtcatgc tcctccatct gctgctcggg cttccagtgt cgacctgccg ccaatgtacc
    14461 cgtacccgtc ttgccagccg gatgcggccc ggggattggt tcgaatgcgg ctcgccgggg
    14521 ccgatgttgt gcttggctgt tgctagagga gaaggaggaa taaatggcaa gtagccaacg
    14581 ggtcctgggc ttggccctga ccgcgatcct gctcagtgga tgtagcgccc ccgctccggc
    14641 aaccgtcacc cagggcacgg acgcgccggc aagttcggcc gctgcgccgc agacgtcggt
    14701 ggccgcgccg gcgggcgatt ccggcaccga cgagttcgtg tcgaaactct ccaccgcgat
    14761 ggccggggtg aagacgtaca gcttcgacat cgacatggag accgaggtct ccgggcaggc
    14821 cgcggtcatc aagaccaagg gcgttctgga ccagaccgac gcggccaaca agaagatgca
    14881 gatggacgcg aacatcgccg gcatggccat gaagatgctg caggtcgatg gcgagctgta
    14941 tgtgcagatg gaggtgctcg gcgacaagtg gatgctggtt cccgaggacc agcgctccaa
    15001 ctacatggag gccaccaaga ccggcgacat ggccaagtcc ttcgagcagt ccaaggatct
    15061 gatcaagagc ctcgagacgg tcggcgacga gaccgtggat ggcgtggcca cgaagcacta
    15121 cctggtcacc tatgacggct cggcgctgtc aggcctcacc ggcggtacag gatcggtcga
    15181 aggcaccctg gagtaccaca tgtgggtgga cgcggattcg ctgatccgga aggtctccat
    15241 ggaggccgcg gcgagcgagg acagcgaggt ggttccgttc aagatgagcg gcaccatggg
    15301 tgattacaac gagccggtga ccatcaaggc gccgagcaag gacgacatca ccgagatgcc
    15361 gagctgatcg gcgacctctg tcattccggt ccaggctcaa cgtcggctct tcggggcgcc
    15421 atcgagccac catcctcgcc gagcgccagc cgtccgtatc gagcgccagc cactggtgct
    15481 ggcgctcgat accggaggct ggtcctcgcc gggctcactc acccacgggg tggttgagcc
    15541 tgatcgctca cttgccgagg gccttgagga tgtcgtccac gcgctccttg gcgtcgccga
    15601 agagcatgtg ggtgttctcc ttgaagaaca gcgggttctg gacgccggcg tagccggtgg
    15661 ccatcgaccg cttgaacacg atcacgttct cggccttcca cacctcgagc accggcatgc
    15721 cggcgatcgg ggagttcggg tcctcgagcg ccgcaggatt cacggtgtcg ttggcaccga
    15781 tcaccagcac gacgtcggtt tcggcgaagt cgtcgttgat ctcgtccatt tccaggacga
    15841 tgtcgtaggg gacgtgagcc tcggccagca ggacgttcat gtgaccgggc aaccgtccgg
    15901 cgaccggatg cacgccgaat cggaccttca ccccgtgagc gcgcagcttc gcggtgaggt
    15961 cagccaccgg gtactgcgcc ttggcgacgg ccatgccgta gcccggggtg atgatcaccg
    16021 acttggcgtt ccagagcatc tcggcggtgg tggctgcgtc gatctcgcga tgttcgccgg
    16081 tctcctccgc acctgagctg acctggccgc cttcactgcc gaacccgccg agaatcaccg
    16141 agatgaacga acggttcatt gcccggcaca tgatgtagga caggatcgca ccggaggagc
    16201 ccaccagtga gccggtgatg atcagcaggt tgttgttcag catgaagccg gctgcggccg
    16261 ccgcccagcc cgagtacgag ttgagcatgg agaccacgac gggcatgtcg ccgccgccga
    16321 tcgccgcgac catgtggtaa ccgagggcga agccgatcag ggcgatggcg atgatcagga
    16381 cccagccgag gagcgcgttg tgctcgccga cggtgacgaa ccacaccatc agcgcgagga
    16441 agcccacgaa cccggtcaga ttgatcaggt tgcgcccggg cagcatcagc ggctgcgact
    16501 tcatcttcgc cgacagcttc aggtaggcca ccaccgagcc ggtgaacgtg taggcgccga
    16561 tcagcacgcc gagggccacc tcgaccatgt gcactgcgcc gccgccggtc tcgcctggca
    16621 cggcgaagga gttgaagccg accagaaccg ccgccatgcc gacgaaggag tggaaggcag
    16681 cgatcagttc gggcatctgg gtcatctcga ccttgcgcgc gatcggggtg ccgaggaagg
    16741 cgccgatgcc cagcacggcg acgatcagca ccagggttac ccagaccggc agtcgatcat
    16801 cctcggtggc tgagatgttc agcgaatgga tcacggtcgc caccagagcg agccccatgc
    16861 cgatcatgcc ggcgacgttc cccaagcgag ccgtggtctg cttggacagg ccggccaggg
    16921 aaaggatgaa caggacgccg gcaacgatgt acaggccctg gacgaactgg agcagcatgc
    16981 tcacgcctcc ttcttgaaca tgctgagcat ccggtaggag accaggaagc cgccgaaaat
    17041 gttgatggac gcgatggtgg ccgcgatgaa agacatcacc agcaccgcga tgtttgtcga
    17101 gccgagttgg aggagcgcgc cgaccaagat gatgcccgag atcgcgttgg tctgggccat
    17161 caggggcgtg tgcagcgagt gcgaaacgcc gctgaccaca tagaacccga ccacgaccgc
    17221 gagtgcgaac accgtgaacg cgccgacgaa gctggccggt gagaaggtga ccgccaggat
    17281 caccagcgca gcggcgatcg ccgacatcac gatccggttc cgagccttgc cggcagccgc
    17341 gcgggcttcc cgggccgtcc ggaccgccgg atcctcggcg ggctcgacca cagccgggac
    17401 cgccgcagcc gagacttgga ccggtggggg cggccacaac agctcgccgt tgtcggtcac
    17461 cgtcatacct cgaactacaa cgtcgcccag atcgacggtg acctcgccgt ccttggccgg
    17521 cgtcagcagc ttgaacaggt tcaccacgtt ggtgccgtag agctgcgatg cctgggttgg
    17581 gaggcgtccg gcgaggtcgg tgtacccgat gatcttcacg ccgttggtgg tggtcaccac
    17641 ctcgtccggc cgcgaaccct ccacgttgcc gccgttggcc gcggccatgt ccacgatcac
    17701 cgaaccggga cgcatcgagg cgaccatgtc ggcggtgagc agccgcgggg cgggcttgcc
    17761 ggggatgagc gcggtggtga tgacgatgtc gacgtccttg gcctgctcgg cgtacagctc
    17821 ggcggccgcg gccttgtagt cgtcgcccat ctccttggcg tagccgtcgg cggagacctg
    17881 ctgggtgatc gcccgcggcg acacgaactc gccgcccatc gacgcgacct gctcgcccac
    17941 ctcgggacgg gtgtccgtgg cgcgcacgat cgcacccagc gacttggccg tgccgatcgc
    18001 ggccagcccg gcaacgccgg cgcctgcgac cagcaccttc gccgggggca ccttgccggc
    18061 ggcggtcact tggccggtga agaacgagcc gaactcgttg gccgcctcga ccacggcacg
    18121 gtagccggcg atgttggcca tggaggacag cacgtccagc gcctgggcgc gggagatccg
    18181 cggcaccgcg tccatcgcaa ggacggtcag cttgcgctgg ttcagcttgg ccaacagttc
    18241 cgggttgcgg gcgggcgcga tcaacgagat cagcatcccg ccttcgggca gctgtccaat
    18301 ctctgcgtcg gtcggggcgt tgatcttcgc cacgaccggc gctgaccagg ccttcgcccg
    18361 gccgacgatg gtggcgccgg cagcggcgta ctcggcgtcc gggtaggccg acttggcgcc
    18421 ggccccggcc tctacgatca cctcatggcc gagcttgatg agttgggcga cggtcttcgg
    18481 ggtggctgcc accctggtct cgtcgtggag ggtttccagg ggaatcccga tctgcatgtc
    18541 ctgagagtcc ttccggtgga gtcggtctgg tacccgaccg cgctagcgta gtgctcagaa
    18601 ttgcgaccgc ctgcgattag ggcgccgatt ggcgcgcccg ccggtgccca accggctgat
//
LOCUS       DAOPRQ010000052        18128 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_2604_length_18128_cov_12.337022, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000052 DAOPRQ010000000
VERSION     DAOPRQ010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 18128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18128
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2604_length_18128_cov_12.337022"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3149)
                     /locus_tag="PKJ61_08005"
     CDS             complement(<1..3149)
                     /locus_tag="PKJ61_08005"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HOC13808.1"
                     /translation="MSLKLRRAQSTGVVRALVALLAALGLLVGLSPSRAMAATDTGTI
                     SGVVTSSAGAITGAQVMLYNALDGGGYDLETTTLADSSGQYVFFEVPAGTYLVYFSAT
                     DHVSEWFDNVVSGEPTTVTVTAGATKIVSAQLAALPKITGTIYDPNGAVAAGASVCVA
                     NSYDPSYCTYVTSDSSGKYQISRYPGTYLVRAITSNTLDTYFAGATSVLDSSAATKVT
                     LTSGGADATGKDIHLIQGATISGTVTRAGAAVTNARVCAANGACAYTDQGGYSLQVRA
                     GTTKLTVSWWDSATGTYLNGVTQLSVVDKQSYPNTNIALSEGTGVSGTVTLDSLANGA
                     SIDVYRIAADGTERYSDYDYTRGTNNGFIFRDLEPGTYVVKVAAAGFASSCFGGANCT
                     KFTVTKDAITPVGNLALTRLAPGGLTGKVLGSDGSGLAGVTVKATWNSTSFTTLTGGD
                     GDYSFSGLAAGVGWQLSLEKSGYLTKSLWATPKASKSVRLDSIRLLKPSGISGKVTDP
                     AGAGVQTTLYLSGPGYYNATSDASGNYSLPAITPGRYTVYIYAASGLLSLSKEVAVGD
                     GKQVTADFRLQRPATLSGTLLKAGGAQLSGCVTLYTKDGDDVKEACADLSGIFSFTSV
                     RPGTYTIQGYQDGYRDTWLGDVASMSKATWFTLADGEAATGKVITLQPLPGGTVSGTV
                     KLSDGTAAKGLEIALLSDDEEDPDEVSVGADGSYDVGRVPSGTYSVRMYWEGRICGPD
                     YAAQCSMATVTVGTTDQTVDFVLANLGSVSGSLTGADGNAVDWAELHLTDATDEKIAY
                     VDLERESDFTFAHVPFGTYTLSAEANDSIIHRTVVVDGPETQNLQFVTGHSITGTIHT
                     TSADYYANVRAIDPVTGETLAHDDVEVEGNDVDLSYELRHLPPGDYLIYVSSDAGDLW
                     YPAASNPRQASPVTITNADRTGVDFTVAAPSGWATVTGTLRLPAGVTADSRYDYPQLE
                     FRSDDHYYEVEAGADGSYRLSMALGDYHVRIDGSDELGTAGQESDLTISGDTTLDFTL
                     AAAGSMIGQVVDGNDVGIEA"
     gene            complement(3412..4338)
                     /locus_tag="PKJ61_08010"
     CDS             complement(3412..4338)
                     /locus_tag="PKJ61_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOC13809.1"
                     /translation="MRALRRGFLATGTALLIALSTLTFASPAHAEETFSLSGTLTDAS
                     GPLGGLNTCADPINYDINTNPRRECVTSAADGSYTITGLRPGEYTLTAWGDGYPMRFF
                     NTGGSTSNLADASIIRLAQSLAGQNITLMRAPALSPAPRPTVSGKAKPGAKLTAKPGD
                     WAGATLSYQWLRAGTAIAGATKATYKVQPIDGGATLQVQVTGSKPDYSPTVRVSDATK
                     VVPLSKLKSAKPKIAGKATIGKMLKVKPGKWTAGTKLVTQWLRGKTVVASGASYLVAP
                     ADLGQKLTVRVTGTKQGYKTVAKTSKATKRVK"
     gene            4554..6206
                     /locus_tag="PKJ61_08015"
     CDS             4554..6206
                     /locus_tag="PKJ61_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13810.1"
                     /translation="MPSSSRLPRWTASIATAIALSFTPLAPAPVAQAKRPAAPASLAS
                     AAIDAHLGYQAQSTCSPSPKPGTTALLRLLIKTWGGKSSGIYRGCSSGGTSEHKEGRA
                     LDWRMSAKSASQRKRVDQALAWITANNGEVAYRLGVMYIIWNQRIWSIYYPELGWRKM
                     ASRGSATANHKDHVHISLSWDGAMKQTSWWTGVPVTQPLNSKCGTNGARACLSTIARR
                     SGSAWPYLASVVPATFLPFPWTKPAIGGSPQVGRTLTAVPGTWVPEGATLSYQWTADD
                     KPILGANQPTYVVTTGEVGKEIRVVVSATSAAGVRTLTSDELAEVYRGRFGNASPVIL
                     GTIAPGTTVTADVSGWSPAPTRASYRWLRNGKAIKSATKATYTITGADLGKKLSVRVT
                     AGATGYYDLTATSAATKVAPAFASAPLPTVTGTPVVGGTLTVDAGVWKPTPAQLSLQW
                     LVDGAAIADATGATLALGPELLGRTVAVRVRAELAGVASMSTVSAGVVVAAPAAPAAP
                     EPEVAAPARPVPEVVTPEPEAVTPAPEVAVAEAAADDGDGPS"
     gene            complement(6259..>7731)
                     /locus_tag="PKJ61_08020"
     CDS             complement(6259..>7731)
                     /locus_tag="PKJ61_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13811.1"
                     /translation="LGALSRWRLEPKPERLADLEFAGYRRQTASSIAFSPAGDELALG
                     FKAQALLRWRLEGDRADALPKLTGFTDWIEAVDYSADGRQLVAASLDQSTTVFDRDGN
                     RLRRLPGPSALTGAAFIGDRLVTTAKDGTLRVWADAGPRWRYAGSELYNLSTDASAQM
                     WLAGGTPYDGIQLWRLAATNGRTTVEHERKPVPRTGLATGVQTGAVAIGPRGDFLLGG
                     TIDGRVIHWPLGPDGAGEPSFLGLGIGYIAFVQASPDSKLAVVMGYQGKKSALLSLAE
                     DGRPRLAAELPVDDPQMFWFSADSRLLAIALPDRSVRLWDVSTPGSPAEVGAIRELPS
                     TPVSVAFAPHSRLLAVGTDLGAVSVWDVSRPEAPVQRRVFSDPHAATNAVLFSPDETM
                     LVATGGDQVIRGWDLTSSSDSGPVFALSGDLGRPWDARFLPGDRLAVSGGNGALQVWT
                     LSVTAARQQLCAVRGDQLRPEEWSSYLPGIPVSDPCAAVR"
     assembly_gap    7732..7831
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7832..9220)
                     /locus_tag="PKJ61_08025"
     CDS             complement(<7832..9220)
                     /locus_tag="PKJ61_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13812.1"
                     /translation="MSSSVSRQAFAARLDQERRRRHLSIRAIAKVADVPATTAQGWLN
                     GRHFPTPALRGNFLRLVTELELAHAMPDDLWADSLGGIAPTLRAGNSPYLGLRPFAVT
                     DSELFYGRASQSGRIADAVLEMAETEGHGMIALVGPSGSGKSSVLAAGLLGKEVSEGP
                     LLGWTGVQLSVDELRAGVASVAVTAGRRLAVVDHFEDAILLPAEDRSRVLTELVRVAH
                     DAVVVLGLRSDAFADAALEPSLEAAMSRPILLAPMTRGELRDVIVRPAESLGLEVDDN
                     LVRVLLDELAPGPPDTRVAPGVLPLLSNALLVIWAVGGGQRLTLDDYHRIGGVATTVE
                     QLAEQVFSSLDAAGQEAAKRLFLRLVHTTGDVVGRDSVLLGEIDTATRPVLDAFVAAR
                     MLTIVDDSVRISHLALLSHWQRLIGWLEQHRDDVIVLRRLRHAAEVWRENDRDSAALI
                     PVGRLALFTDWLA"
     gene            complement(9315..9560)
                     /locus_tag="PKJ61_08030"
     CDS             complement(9315..9560)
                     /locus_tag="PKJ61_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOC13813.1"
                     /translation="MAHRPTRSELTELINQARSERHISIRAAARIAGVPPATMQGWLS
                     GRHFPTHALRPKFLRLLESLDLNHLVHPALWLDDASP"
     gene            complement(9777..11399)
                     /locus_tag="PKJ61_08035"
     CDS             complement(9777..11399)
                     /locus_tag="PKJ61_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015071405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOC13814.1"
                     /translation="MKTVIVGGVAGGMSAATRLRRLDETAEIVVLERSGHVSFANCGL
                     PYYIGGVITDRNALLLQTPASLKARFGLDVRVDSEVTAIDRDAKTVAIADLATGKTYT
                     ESYDQLILAPGAAPFVPTIPGVERALALRNIADTDKLADAVATALTKPDPTAVIMGGG
                     FIGIELAENLTERGLKVTVVELANQILAPMDVEMAALVEKHLAKHDVTVVTGAAVSEI
                     GTDEVTLSTGATLPATLVIAAIGVRPETGLAEAAGLALGERGGILVDAQQRTSDPAIY
                     AVGDAVVKVDALTGGQALVPLAGPANRHGRLVADVIAGREVRASGVQGTAILGAFGLS
                     AAATGWTEKRAVAAGRKIRVIHTHPANHAGYYPGAEGMALKLVVDAETDAILGAQGVG
                     GSGVDKRIDVIATAMRGGLTASELAELELSYAPQFGSAKDPVNMLGFIAENLRDGLTE
                     TVQWHEVDAEVARGVQLIDVRTTTEYGRGTVSGAINIPVDDLRARLDEVGDDVIVHCQ
                     VGLRGYIAARTLAQTGRRVRNLDGGYRTWARI"
     gene            complement(11592..13394)
                     /locus_tag="PKJ61_08040"
     CDS             complement(11592..13394)
                     /locus_tag="PKJ61_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014847341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HOC13815.1"
                     /translation="MDAGHLAARFLASAAEHAQRPATRVLAPDADQSETPVWRVRTYA
                     QLAADVRSLAAQLIASGLAPGDRVAIFSPNLPEWSLVDLACLAAGLVVVPLYDTSTED
                     QARHILADSGSRWVFVGSKAGAAKVAAIRDDLPSLERVVSFTEVSDAIPTLAAELAAA
                     PDDLSAVDRRLADASAADLATIIYTSGTTGQPKGVMLSHRGFTHQVDVLTRYLPISAD
                     DSSLCFLPLSHALERAWTYEVLCNGCLNTYVPDARTVAEMMVKAEPTMLVSVPRLYEK
                     VHLAAQEKVAGSPLKQKLMAWALKVGTEFHTAVHAGKPRSKALIVKFKLADKLVLGNV
                     RHAMGGPKTVLACGGAALRKEVEEFLFACGLLVLQGYGLTEASPLVTFPPPYAYRFGT
                     VGQVMEGGEIALGEGGEILYRGPNLMLGYWNRPDDTASMLVDGWLRTGDVGEVDADGY
                     LKVTDRIKDLIVTSYGKNVAPAPIEGLLASDPLFEYTLLLGDNRPYLTLLVSPSMPHL
                     EEIAKQLQVSWAHRDELFNHPEVLAEIKRRVGELTEKLANHEQIRDLRLLVEGFTQEN
                     GLLTPTLKVKRREVEKRFAQLVDDMYARVGMPRK"
     gene            13506..14300
                     /locus_tag="PKJ61_08045"
     CDS             13506..14300
                     /locus_tag="PKJ61_08045"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017933517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HOC13816.1"
                     /translation="MLTMGAHVDQTDPIAEALAREATASQFFLGDPQAWKGPKVAYAG
                     GAAQLKADAEAAGVALYVHAPYVLNVATTNNRIRIPSRQHLQKQLTAAAEIGAAGVIV
                     HGGHVLASDDPAIGFDNWRKAIDGLTIEVPLLIENTAGGANAMARQLDRLAQLWDAIS
                     LSANADRVGFCLDTCHAHAGGIELGGLAEKILAITGRIDLVHANDSRDAFDSGADRHT
                     NFGSGNLDLVAAADLFRDAKAPLIIETPGPLEAHRRDLAWLAEVTA"
     gene            14379..16451
                     /gene="pta"
                     /locus_tag="PKJ61_08050"
     CDS             14379..16451
                     /gene="pta"
                     /locus_tag="PKJ61_08050"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020385064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HOC13817.1"
                     /translation="MSNSIYVASPEGLTGKSMVAVGMIEALTRTVTSVGVFRPVVRAG
                     VEDAILHTLVSQPGIFQTYEEAVGVTYDDVHRDADAALSVLVEKYSEIKERYESVVIL
                     GSDYTDVTSPIELTFNARVAANLNAPVILVVSGKGRAAEGIRPVAEGALAEFRARHAK
                     VIGVIANRVDPGQLDATRAELAKLPDVVTGALPADRFLAAATVRAQFEAVQANLVLGN
                     PELLGRESATVLVAAMTLPNVLARLEADSTVIMPGDRCDMLPGLLLAHASGSFPQLAG
                     IILLGGYEIPETISKLVSSIHNELPIGMTHLGTFTSADKMFRLEGAMTSSPRKVEMAR
                     RIFSEFVDVPALLRALEVHRSEVMTPLMFEHQLQEIARSDRRTIVMPEATDDRILESA
                     DILLRRGVAKLILLGDALMIKQRATHLGLGLHGVEVVDPTDPELVERFAAEYARLRAH
                     KGVTLEQARERLTDLSYFGTMMVHLGMADGMVSGAINTTANTIRPSLEFIKTKPGVTV
                     VSGSFLMCMPDRVLVYADCAVNPDPTAEQLADIAISSAETAVAFGIEPRVAMLSYSTG
                     TSGSGADVDKVREATAIVRERRPDLALEGPIQFDAAVDPIVAKTKLPNSPVAGKATVF
                     IFPDLNTGNNTYKAVQRSSGAVAIGPVLQGLNKAVNDLSRGALVEDIVNTVAITAIQA
                     GQESPRNN"
     gene            16463..17647
                     /locus_tag="PKJ61_08055"
     CDS             16463..17647
                     /locus_tag="PKJ61_08055"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013159995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HOC13818.1"
                     /translation="MSTPILLLNCGSSSIKYQVIDADDEDVLASGIIQRIGQPESTLD
                     HKFGDQTRHDDHGFPNHARALAHLVRVFNEVGPNLADVVAVGHRTVHGGEAFRETVVI
                     DDEVLAKLRELSPLAPLHNPPGIAGIEAAREVLPDVPHVAIFDTAFFATLPAEAYTYA
                     IDAELAAEHGIRKYGFHGTSHSFVSKKVAEYLGRDLGSLKQIVCHLGNGASISAIDGG
                     VAVETSMGLTPLAGLVMGTRSGDVDPGLHAFLGRTLGLDLGGVDDLLNKKSGMLGLVG
                     VTDFRDLGELIDAGDAKAQLAMDVYVHRLVFYIGAYHALLGGLDALTFTAGVGENDDL
                     VRRLVVSRLGALGIELDSDANQVRAKHPRTISTPASKVTVLVVPTNEELAMARETKTA
                     IS"
BASE COUNT         2975 a         6115 c         6012 g         2926 t
ORIGIN      
        1 gcctcgatgc ccacgtcatt gccatccacc acctggccga tcattgagcc agcggcggcg
       61 agagtgaagt cgagggtggt gtcgccggag atggtcagat ccgattcctg acccgccgtg
      121 ccgagttcgt ccgaaccgtc gattcgcacg tggtagtcac cgagagccat gctgagtcga
      181 tagctgccgt ccgcgccggc ctcgacctcg tagtagtggt cgtcgctgcg gaactccaac
      241 tggggatagt cgtagcgact gtccgcggtc acgccggctg gtagccgcag cgtgccggtg
      301 accgtcgccc aacccgatgg cgcggccacg gtgaagtcaa caccggtgcg gtcggcattg
      361 gtgatggtga ccgggctcgc ctggcgcgga ttcgacgcgg cggggtacca caggtctccg
      421 gcgtcgctgc tgacgtagat caggtagtca ccaggtggta agtggcgaag ctcgtagctc
      481 aggtctacgt cgttcccttc tacctccacg tcgtcgtgag cgagagtctc tccggtgacc
      541 gggtcgatcg ccctgacgtt ggcgtagtaa tcggctgacg tggtgtggat cgtcccggtg
      601 atcgagtggc cggtgacaaa ctgcaggttc tgtgtctccg ggccgtccac caccacggtg
      661 cgatgaatga tcgagtcgtt ggcttcagcg ctcagcgtgt aggtgccgaa gggcacgtgt
      721 gcgaaagtga agtcgctttc gcgctccaga tcgacgtagg cgatcttctc gtcggtggcg
      781 tcggtgaggt gtagctcggc ccagtccacc gcgtttccgt ccgctccggt cagggagcca
      841 gacactgaac ccaggttcgc caggacgaag tcgaccgtct ggtcggtggt gcccaccgtc
      901 accgtggcca ttgagcactg ggcggcgtag tcaggcccgc agattcttcc ctcccagtac
      961 atccgcaccg aataggtacc cgagggcacc cgcccgacgt cgtagctgcc gtcggcgccg
     1021 acggacacct cgtcggggtc ctcctcatcg tcgctgagga gcgcaatttc caggcccttt
     1081 gccgcggtgc cgtcgctcag cttgaccgtg ccgctcacgg ttccgccagg cagtggctgg
     1141 agggtgatca ccttgccagt ggcggcttcg ccgtcggcga gggtgaacca ggtggccttc
     1201 gacatcgagg ccacgtcacc gagccacgtg tcccggtacc cgtcctggta gccctggatg
     1261 gtgtaggtgc ccggacgtac cgaggtgaac gagaagatgc cgctgaggtc ggcgcaggcc
     1321 tccttcacgt cgtcgccatc cttggtgtac agcgtgacgc aaccgctcag ctgagcaccg
     1381 ccggccttga gcagcgtgcc ggacagggtg gccggtcgct gcagccggaa gtcggccgtc
     1441 acctgcttgc cgtcacccac tgcgacctcc ttgctcagcg aaagcaagcc cgaggcggcg
     1501 tagatgtaga cggtgtagcg cccaggggta atggcgggga gggagtagtt cccactggca
     1561 tcgcttgtgg cgttgtagta ccccggaccg gagagataaa gggtggtctg cactcccgcg
     1621 cccgcagggt cagtgacctt gccggagatg ccggatggct tcagcagcct gatcgaatcc
     1681 agacggaccg acttgctcgc cttcggtgtg gcccacaggc tcttggtgag gtagccggac
     1741 ttctccaggc tgagctgcca acccacgccg gcggccaaac cgctgaagga gtagtcgccg
     1801 tcgcctccag tgagcgtcgt gaacgaagtg gagttccagg ttgccttgac cgtcacgccg
     1861 gccaaaccgg aaccgtcgct accgaggact ttgccggtaa gtccacccgg agcgagccgg
     1921 gtgagcgcca gattgccgac cggggtgatc gcgtccttgg tcacggtgaa cttggtgcag
     1981 ttcgcgccgc cgaagcacga ggaggcgaaa cccgccgccg ccaccttgac cacataggtg
     2041 ccgggctcga ggtcgcggaa gatgaagccg ttgttggtgc ctctcgtgta gtcatagtcc
     2101 gagtagcgct cagtgccgtc ggccgcgatg cggtagacgt cgatgctggc gccgttggcc
     2161 aggctgtcga gcgtgaccgt gccgctaacc ccggtgccct ccgacaacgc gatgttggtg
     2221 ttggggtagc tctgcttgtc gacgacggag agttgcgtga ccccgttcag gtaggtgccg
     2281 gtggccgagt cccaccagga gacggtgagt tttgtcgtcc cggcccgaac ctgcaggctg
     2341 tagccgcctt ggtcggtgta tgcgcaagct ccattggccg cgcagacccg cgcgttagtc
     2401 actgcagccc cggcgcgagt gactgtaccg gagatcgtgg ctccctgaat caggtggatg
     2461 tccttgccag tggcatccgc tccgcccgag gtcagcgtca ccttggtcgc agcacttgaa
     2521 tcgaggacgc tggtcgcacc cgcgaagtag gtgtccagcg tgttgctggt gatagcgcgc
     2581 accaagtacg tgcccgggta acgcgagatc tggtacttgc ctgaggaatc ggacgtgacg
     2641 taggtgcagt agctagggtc atacgagttg gcgacgcaga ccgaggcccc cgcggccacc
     2701 gctccgttgg ggtcgtagat ggtgccggtg atcttgggca gcgcggcgag ttgggcgctg
     2761 acgatcttgg tggcgccggc ggtcaccgtc accgtggtgg gttcgcctga aacaacattg
     2821 tcgaaccact cgctgacgtg gtcggtggcg ctgaagtaga ccaagtaggt tcccgccgga
     2881 acttcgaaga agacgtattg cccggacgaa tcggcgagcg tcgtagtctc caagtcgtag
     2941 ccgccgccat cgagggcgtt gtaaagcatg acctgcgctc cggtgatcgc ccctgcggaa
     3001 ctggtcacga caccgctgat cgtgcccgtg tcggtagcgg ccatagctcg gctcggcgag
     3061 aggccgacca gcaggcccaa cgctgcgaga agggcgacga gggctcggac aacacccgtg
     3121 gactgtgcgc gacgtagctt cagcgacatt tcggtcttcc tttcgcccgc tacccgccga
     3181 ccttccccgg cctgcgcccc acgggccacc cccgagatca tcgagcctca actcaggccg
     3241 aagtgaccat ctcgccgagt tcgtacgagt ccatacacct agcgacaaca gcagccatgg
     3301 gggtgtctac gcgcgcggac gcgtccgcgg cgaatcgagc gctcggccat cgatatgcgg
     3361 gttctgaacg ggcaatccgg gctcagaacc cgcatttcga tggcgagacg gctacttcac
     3421 cctcttggtc gccttcgagg tcttcgcgac cgtcttgtag ccctgcttcg tcccggtcac
     3481 cctgaccgtg agcttctggc ccaggtcggc tggggccacc aggtagctgg cgccggaggc
     3541 aaccacggtc ttgccgcgaa gccattgggt gaccagtttg gttcccgcgg tccacttgcc
     3601 cggcttgacc ttcagcatct ttccgatggt cgccttgccg gcgatcttcg gtttcgccga
     3661 cttcagcttg gacaacggga cgaccttggt cgcgtccgag acccggacgg tgggactgta
     3721 gtcgggcttc gaaccggtga cctgcacctg gagggtggcg ccaccgtcga tcggctgcac
     3781 cttgtaggtg gccttggttg ctccggcgat cgccgtcccg gctcgaagcc actggtagct
     3841 gagggttgcg ccggcccaat cgcccggctt cgcggtgagc ttggcaccgg gcttcgcctt
     3901 gccgctgacc gttggcctgg gcgccggact cagcgcaggt gcccgcatca gggtgatgtt
     3961 ctgaccggcc agcgactgtg cgagccggat gatgctggcg tccgccagat tggaagttga
     4021 accgccggtg ttgaagaagc gcatgggata gccatcaccc cacgcagtca aggtgtactc
     4081 gcccgggcgc aggccagtga tggtgtatga accgtccgcg gccgaggtga cacactcgcg
     4141 gcgtgggttg gtgttgatgt cgtagttgat cggatcggcg caggtattga gcccgccgag
     4201 cggaccggac gcgtcagtca gtgttccgct gaggctgaag gtttcctctg cgtgagccgg
     4261 ggatgcgaaa gtgagggttg aaagtgcgat gaggagagcg gttccggtag ccagaaaacc
     4321 gcggcggagt gcgcgcatgg cagattcctt gggggagggt agccgggagg ggttgcccga
     4381 cagccaaagt attgcggaat ccgggcagct accgcgaggg gttcaggcaa tccgtagtgc
     4441 ccgggatcga aatgcgggtt ctcagcccaa atacaacctc cagaacccgc agatcaatgc
     4501 ccgaacacac cggacggctt gacgatccgg ctgatcacac cgagtcttgc tgggtgcctt
     4561 cctcctcccg gctgcctcgg tggacagcgt ccatcgcgac cgcaatcgcg ctcagcttca
     4621 ctccgctcgc cccggctccg gtcgcccagg ccaagcgtcc ggccgcgcct gcgagcctgg
     4681 cgtccgcagc catcgacgct cacctcggct accaggcgca gtcaacctgc tcgccgtccc
     4741 cgaagccggg gacgaccgcc ctgcttaggc tgctgatcaa gacctggggc ggcaagtcgt
     4801 ccggcatcta ccggggttgc agttccggcg gcacgtccga gcacaaggag ggacgcgcgc
     4861 tcgactggcg gatgagcgcc aagtcggcat cgcagcgcaa gcgcgtcgac caggcgctgg
     4921 cctggatcac cgccaacaac ggcgaggtcg cctaccgcct gggcgtgatg tacatcatct
     4981 ggaaccagcg gatctggtcg atctactacc ccgaactcgg ttggcggaag atggccagtc
     5041 gcggttcggc caccgccaac cacaaggacc acgtgcacat ctcgctgtcc tgggacggcg
     5101 cgatgaaaca gacctcgtgg tggacggggg tgccggtcac ccaaccactg aacagcaagt
     5161 gcggcaccaa cggcgcccgt gcctgccttt cgacgatcgc ccggcggtcc ggatcggcct
     5221 ggccctacct ggccagcgtc gtgccggcca ctttcctgcc cttcccttgg accaagccgg
     5281 ccatcggtgg cagcccgcag gtcggacgga cgctgaccgc cgtccccggc acctgggtgc
     5341 ccgagggtgc gaccctcagc taccagtgga cggctgacga caagccgatc ctcggcgcca
     5401 accaacccac ctatgtggtc accaccggcg aagtcggcaa ggagatccgg gtggtggtgt
     5461 ccgctacgtc ggctgccggc gtgcggacct tgacctcgga cgaactcgcc gaggtctacc
     5521 gcggacgctt cggtaacgcg tcaccggtga ttctcggcac catcgcgccc ggcacaacgg
     5581 tgacggccga tgtcagcggc tggtcaccgg cgccgaccag ggccagctac cgctggctgc
     5641 gcaacggcaa ggccatcaag tcagccacca aagccaccta caccatcacc ggcgccgacc
     5701 tgggcaagaa gctctcggtg cgggtgaccg ccggcgcgac cggctactac gacctgactg
     5761 ccacgtccgc cgccaccaag gtcgcgcctg cgttcgcgtc cgcaccgttg cccacggtga
     5821 ccgggacgcc ggttgtcggc gggaccctca ccgtcgacgc cggagtctgg aagccgacac
     5881 ccgcgcagct cagcctgcaa tggctggtcg acggcgcggc gatagccgat gcgaccgggg
     5941 cgaccctggc gctcggtcct gaactgctcg gacgcacggt cgccgtgcgg gtgcgggccg
     6001 aactcgccgg cgtggcctcg atgtccaccg tttctgccgg tgtggttgtc gctgcgccgg
     6061 ccgctccggc cgctccggag ccggaggtgg ccgcgccggc caggccagtg ccggaggtgg
     6121 tcactcctga gccagaggcg gtcacccctg cgcccgaggt cgcggtggct gaagccgccg
     6181 ccgacgatgg cgacggccct tcgtgacgcg cctcaggaac cgcagcgggt ttcgactgtt
     6241 cgaccggccg agccggactc agcggaccgc cgcgcaggga tcgctgaccg ggattccggg
     6301 caggtagctg ctccattcct ccgggcgcaa ctggtcgcca cggacggcgc acagctgctg
     6361 gcgcgcggca gtcactgaca gcgtccagac ctggagcgca ccatttccgc cggagacagc
     6421 cagccggtcg cctggcagga agcgggcatc ccacggcctg cccaggtcgc cggagagcgc
     6481 gaacaccggg ccggagtccg acgaagacgt caagtcccag ccgcgaatca cctggtcgcc
     6541 accggtggcc accagcatgg tctcgtccgg gctgaacagc acagcattcg tcgccgcgtg
     6601 cgggtcgctg aacacccgac gctggaccgg cgcctccgga cggctcacgt cccagacgct
     6661 gaccgcgccc agatccgtcc ctaccgccag cagccggctg tgcggagcga aagccacgga
     6721 caccggggtg ctcggcagct cgcggatcgc gcccacctca gccgggcttc ccggcgtact
     6781 cacgtcccag agccggacgc tacgatccgg cagcgcgatc gccagcaacc gcgagtcagc
     6841 gctgaaccag aacatctgcg gatcgtcgac cggcagctcg gccgccaacc gcggccgccc
     6901 atcctcggca agcgacagca gcgccgactt cttcccctgg taccccatca cgacggccag
     6961 cttcgagtcc ggggacgcct ggacgaaggc gatgtagccg atacccaggc cgaggaacga
     7021 aggttcgccg gcgccgtccg ggcccagcgg ccagtggatc acccggccgt cgatcgtgcc
     7081 cccgagcaag aagtcccccc tgggcccgat cgcgaccgcc ccggtctgca ccccggtcgc
     7141 caaccccgtc cgtggtaccg gcttgcgttc gtgctcaacc gtcgtacgac cgttcgtggc
     7201 cgcgagccgc cacagctgaa tgccgtcgta gggcgtgccg ccggccaacc acatttgcgc
     7261 ggacgcgtcc gtactcaggt tgtagagctc cgaacctgcg tagcgccatc tcggccccgc
     7321 gtccgcccag acccgcaggg tgccatcttt cgcggtggtg accaggcggt caccgatgaa
     7381 tgcggcgccg gtgagtgcgg acggaccggg caggcggcgc agccggttgc cgtcccggtc
     7441 gaacactgtg gtgctctggt caaggctcgc cgcgaccagc tgcctgccgt ccgcggaata
     7501 gtccacggcc tcgatccaat ccgtgaagcc cgtcagcttc ggcaacgcgt ccgcacgatc
     7561 accctcgagc cgccagcgca acagggcctg cgccttgaag ccgagggcga gttcgtcgcc
     7621 cgccggagag aacgcgatgc tgctggcggt ctgccgccgg tagccggcga actccagatc
     7681 ggccagtcgc tccggcttcg gttccaaccg ccagcgcgag agcgcaccca gnnnnnnnnn
     7741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncgccagcca gtcggtgaac agtgcgagtc
     7861 ggcccaccgg gatcagcgcg gcgctgtccc ggtcgttctc gcgccaaacc tcggccgcat
     7921 gccgcagcct gcggagcacg atcacgtcgt cgcgatgctg ctccagccag ccgatcaggc
     7981 gctgccagtg actgagcagc gccaagtgac tgattcgcac cgagtcgtcc acgatggtga
     8041 gcatccgtgc ggccacgaac gcgtccagaa cgggacgcgt ggcggtatcg atctcgccca
     8101 acagcaccga gtcgcgtccc acgacgtcgc cggtggtgtg cacgagccgg aggaacaacc
     8161 gcttcgccgc ttcctggcca gctgcgtcca gcgagctgaa cacctgttcg gcgagttgct
     8221 cgaccgtggt ggccaccccg ccgatgcggt ggtagtcgtc cagcgtcagc cgttggccgc
     8281 cgcccacggc ccagatcacc agcaacgcgt tagacagcag cggcaggact cccggtgcca
     8341 cccgggtgtc cggcggaccg ggcgccaact cgtccagcag cacccggacg aggttgtcgt
     8401 cgacctccag tccaagcgac tcggcgggtc ggacgatcac gtcgcgcagt tcgccgcggg
     8461 tcatcggcgc cagcaggatc ggacgcgaca tcgcggcttc cagcgatggc tccagggctg
     8521 cgtccgcgaa ggcgtccgag cgcagaccga gaaccaccac cgcgtcatgg gccacccgga
     8581 cgagctcggt aagcacgcga ctgcggtcct cggccggcag caggatggcg tcctcgaaat
     8641 ggtccaccac ggccagccgt ctgccagcgg tcacagcaac ggaggcgacc cccgcccgca
     8701 actcatcaac cgagagctgg acgccggtcc acccgagcag agggccctcg ctcacctcct
     8761 tgccgagcag cccggcggcg agcaccgatg acttaccgct gccagagggg ccgaccaggg
     8821 cgatcatgcc gtggccctcg gtttcggcca tttcgaggac cgcgtccgcg atccggccgc
     8881 tctggctcgc ccgaccgtag aaaagctcgc tgtcggtcac tgcgaacggc cgcaacccga
     8941 gatagggcga gttgccggcc cgcagggtgg gcgctatccc gccgagcgag tccgcccaga
     9001 gatcgtccgg catcgcgtgg gccagttcga gttcggtcac caggcgcagg aagttgcctc
     9061 gcagtgcggg agtcgggaag tggcggccgt tgagccagcc ctgtgcggtg gttgccggca
     9121 cgtccgccac cttggcgatc gcccggatcg aaaggtgacg gcgtcgtcgc tcctggtcaa
     9181 ggcgggctgc gaaggcctgc cgactgaccg aggaggacat gcgcgaattg tggcagcgtc
     9241 cggctttccc gatagacggt ttcgaacact ttgtacggcg acagcacgcg cccggttcag
     9301 cccagcagcg agtactacgg cgaggcgtcg tccagccaca gggccggatg caccaggtgg
     9361 ttgaggtcga gcgattccag cagccgcagg aacttcggtc gcagggcgtg ggttgggaag
     9421 tgccggccgc tcagccagcc ctgcatcgtg gctggtggga cgccggcgat tcgtgctgcc
     9481 gcacgaatcg agatgtgccg ctcactcctg gcctgattga tgagctcggt caactcggag
     9541 cgggtggggc gatgtgccac ggtggctcct ggggggtacg taccggtgcc gtccggtagg
     9601 aagcgtccgg gattgccggc cttcgactat cgagcgggtg ggactggggt cagtcccacg
     9661 gcactagtga acagttcatg gagtgcccgg acggccaccc tccgccaagt tcgtacacct
     9721 tcgtacaccc cggagggttt gcggccctcg tcaagaggag gcagcgtgat tcgcggtcag
     9781 atccgcgccc aggtgcggta gccgccgtcc aggttgcgga cgcgccgtcc ggtctgcgcc
     9841 agcgtccgcg cggcgatgta gccgcgcaga cccacctggc agtgcacgat cacgtcgtcg
     9901 ccgacctcgt cgagccgggc gcgcaggtcg tccaccggga tgttgatcgc cccggacacc
     9961 gtgccccgtc cgtactccgt tgtagtacgg acgtcgatca actgcactcc cctggccacc
    10021 tcggcatcga cctcgtgcca ctggacggtc tcagtaagac cgtcacgcag gttctcggcg
    10081 atgaagccga gcatgttcac cggatccttc gcggagccga actgcggcgc gtagctcagt
    10141 tccagctcag ccagctcgga cgccgtaagc ccaccccgca tcgcggtggc gatcacgtcg
    10201 atgcgcttgt cgacgccact gccgcccact ccctgcgcgc cgaggatcgc gtccgtctcg
    10261 gcatcgacga ccagcttcaa cgccatgcct tcggcgccgg ggtagtaccc ggcgtggttg
    10321 gcgggatggg tgtggatcac ccggatcttc cgtccggcgg cgaccgcgcg cttctccgtc
    10381 cacccggtag cggcagcgga caacccgaac gcgccgagga tcgcggtccc ctgcactccg
    10441 ctggcacgca cctcgcggcc ggcgatcacg tcggcgacca ggcggccgtg ccggttcgcg
    10501 gggccggcca gcggcacgag cgcctgcccg ccggtcagcg cgtccacctt caccacggcg
    10561 tcacccacgg cgtagatcgc cggatcggac gtgcgctgct gggcgtccac gagaatgccg
    10621 ccgcgctcgc cgagagccag cccagccgcc tcggccagac cggtttcggg gcgaacgccg
    10681 atcgcggcaa tcacgagtgt cgccggcagc gtggcaccgg tgcttagcgt gacctcgtcc
    10741 gtaccgatct cgctaaccgc ggccccggtg accacggtca cgtcgtgctt ggccaggtgc
    10801 ttctcgacca gcgcagccat ttcgacgtcc atcggcgcga ggatctggtt ggccagttcg
    10861 accactgtca ccttcagacc gcgctcggtc aggttctcag ccagttcgat cccgatgaag
    10921 ccaccgccca tgatcaccgc ggtcggatcg ggcttcgtga gtgcggtcgc gaccgcgtcc
    10981 gccagcttgt cggtgtcggc gatgttgcgc agcgcaagcg cccgttcgac gccgggaatc
    11041 gtcgggacga acggcgccgc gccaggagcc aggatcagct ggtcatagct ctcggtgtag
    11101 gtctttcccg tagccaggtc agcgatggca acggtcttcg cgtcccggtc gatcgcggtg
    11161 acctcggagt cgacccgcac gtccaggccg aagcgcgcct tcaggctggc cggggtctgc
    11221 aggagcagag cgttgcgatc ggtgatcaca ccgccgatgt agtacggaag cccgcagttc
    11281 gcgaaggaca cgtgtcccga gcgctccagc accacgatct cggccgtctc atcgagccga
    11341 cgcaacctcg tcgcggcgga cattccaccc gcgaccccac ccacgatcac cgtcttcatg
    11401 gcacctatcc tatacccctc cgggtatcaa cctcgctcgc ggcttcgtgc ctcacctcct
    11461 tgctaccgaa atcgctgttt gctactgaat tttcgggagc aaacgtccgc ttcggtagca
    11521 aacgctgggg tgtgaacggt gggtgcaggc taggggtgga cggcagcgcg cggacgaacg
    11581 gcggacggcg tctacttacg gggcatcccg acgcgggcgt acatgtcgtc gaccagttgg
    11641 gcgaaccgct tctccacctc gcggcgcttc accttcagcg tgggggtgag cagtccgttc
    11701 tcctgggtga agccctcaac cagcaggcgc aggtcgcgga tctgctcgtg gttggccagc
    11761 ttctcggtca gctcgcccac gcgacgcttg atctcggcca gcacctcggg atggttgaac
    11821 agctcgtccc ggtgcgccca actgacctga agctgcttgg cgatctcctc caggtgcggc
    11881 atggacggcg acaccagcag cgtcaggtag ggacggttgt cgcccagcag cagggtgtac
    11941 tcgaatagcg gatcgctggc cagcagcccc tcgatcggcg cgggcgcgac gttcttgccg
    12001 taactggtga cgatcaggtc cttgatccgg tcggtgacct tgaggtagcc gtccgcgtcc
    12061 acctcgccca cgtcgccagt gcgaagccag ccgtccacca gcatggacgc ggtgtcgtcc
    12121 ggccggttcc agtagcccaa catcaggttc ggaccccgat agaggatctc gccgccctcg
    12181 ccaagggcga tctcgccgcc ctccatcacc tggccgaccg tgccgaaccg gtaggcgtaa
    12241 ggcggcggga aggtgaccag cggcgaagcc tcggtgagtc cgtagccctg cagcaccagc
    12301 aggccgcagg cgaagaggaa ctcctccacc tccttgcgca acgcggcacc gccgcaggcc
    12361 aggacggtct tcggaccgcc catcgcatgc cgcacattgc ccagcaccag cttgtcggcc
    12421 aacttgaact tcacgatcag cgccttcgac cgcggcttgc cggcatgcac cgcggtgtgg
    12481 aactccgtgc cgactttcag cgcccaggcc atcaacttct gcttcagcgg gctgccggcc
    12541 accttctcct gcgcagccag atgcaccttc tcgtagagcc gtgggacgct gaccagcatc
    12601 gtcggttcgg ccttcaccat catctcggcg accgtccgcg cgtccggcac ataggtgttc
    12661 aggcagccgt tgcagagcac ctcgtaggtc caggcgcgct caagggcgtg gctgagcggc
    12721 aggaaacaga gggacgaatc gtcggccgag atcggcaggt agcgggtgag cacgtcgacc
    12781 tggtgggtga agccgcggtg ggacagcatc acgcccttgg gctggccggt cgtgccggag
    12841 gtgtagatga tcgtggccag atctgccgcg gacgcgtccg cgaggcgcct gtcgacagcg
    12901 ctgagatcgt cgggggcagc ggccagttcg gcggcgagcg tcggaatcgc gtccgatact
    12961 tcggtgaagc tgaccacccg ctccaggctg ggcaggtcgt cgcggatcgc agccaccttg
    13021 gcggccccgg ccttggaacc gacgaacacc cagcgcgaac ccgagtcggc gaggatgtgc
    13081 cgcgcctggt cctcggtgct ggtgtcgtag agcggcacca ccaccagtcc ggcggccagg
    13141 caggccaggt cgaccaggct ccactcgggc aggttcggcg agaagatcgc cacccggtcg
    13201 cccggtgcca gcccggacgc gatcaactgc gcggcgagcg agcggacgtc cgcggccagc
    13261 tgggcgtagg tgcggacgcg ccagaccggg gtctcggatt ggtcggcgtc cggagccagg
    13321 acccgggtag ccggacgctg cgcatgctca gcagctgagg caagaaaacg agccgcgagg
    13381 tggccggcgt ccattggtgc tcctcacatc gacgtgcacg ccaaacttac tgcagcgtag
    13441 gttcggttca aaggccgatt caggagctcg cgagcaaact gaccgccaat gcctaaagtg
    13501 gagccatgct gaccatgggc gcgcatgtcg accagaccga tccgatcgcc gaagcactcg
    13561 cacgcgaggc cacggcgagc caattcttcc tgggcgaccc gcaggcttgg aagggcccga
    13621 aggtcgccta cgccggcggc gcggcccagt tgaaggcgga cgcggaagcg gccggagtcg
    13681 ccttgtacgt gcacgctccg tacgtgctga acgtggccac gaccaacaac cggattcgga
    13741 ttcccagccg gcagcacctg cagaagcaac tcaccgccgc cgccgagatc ggcgcggccg
    13801 gggtgatcgt gcacggcggg cacgtgctcg cgtccgatga tccggcgatc ggcttcgaca
    13861 attggcgcaa ggcgatcgac ggactgacca tcgaagtgcc gctactgatc gagaacactg
    13921 ccggcggggc gaacgcgatg gcccgccaac tcgaccgcct cgcccagctt tgggacgcga
    13981 tctcactttc ggccaacgcc gaccgggtcg ggttctgcct cgacacctgc cacgcgcacg
    14041 ccggcggcat cgaactgggc ggcctggccg agaagatcct ggcgatcacc ggacgcatcg
    14101 acctggtgca cgccaacgac tcgcgcgacg ctttcgactc gggagccgac cggcacacca
    14161 acttcggtag cggcaatctt gacctggtcg cagcagccga tttgttccgg gacgcgaaag
    14221 cccccctgat catcgaaacc cccggcccgc tggaagccca ccgccgcgac ctggcctggt
    14281 tggccgaagt caccgcctga acggaaaggg gacgtttcag cttggactcc taaccgctgc
    14341 ggcgccgagc acagccggcc agcgataacc tgacgaacgt gagtaacagc atctacgtag
    14401 cctcgcccga gggactgact ggcaagtcca tggtggcggt gggcatgatc gaggccctta
    14461 cccgcaccgt cacgtcggtt ggcgtgttcc gtccggtcgt ccgcgccggg gtcgaggacg
    14521 cgatcctgca caccctggtg agccagcccg gcatcttcca gacctacgag gaggcggtcg
    14581 gcgtcaccta cgacgacgtc caccgcgacg cggacgcggc cctgagcgtg ctggtcgaga
    14641 agtactccga gatcaaggag cgctacgaat cggtggtgat cctcggctcc gactacaccg
    14701 atgtcacctc gccgatcgag ctcaccttca acgcccgcgt cgccgccaac ctgaacgccc
    14761 cggtgatcct ggtcgtctcc ggcaaggggc gcgcagccga gggcatccgt ccggtagctg
    14821 aaggtgcact ggccgagttc cgcgcccggc acgcgaaggt gatcggcgtg atcgccaacc
    14881 gcgtcgaccc gggccagctg gacgccaccc gagcggaact ggcaaagctc ccggacgtgg
    14941 tgaccggcgc gctgccggcc gaccgattcc tggccgcggc caccgtccgc gcccagttcg
    15001 aggcggtgca ggcgaacctg gtgctgggca accccgaact gctgggacgc gagagcgcga
    15061 ccgtcctggt ggccgcgatg accctgccca acgtgctcgc ccggctggag gccgactcga
    15121 ccgtgatcat gccgggcgac cgctgcgaca tgctgcccgg cctgctgctc gcgcacgcgt
    15181 ccggatcgtt cccgcagctg gccggcatca tcctgctggg cggctacgag atcccggaga
    15241 cgatcagcaa gctggtcagc agcatccaca acgaactgcc gatcggcatg acccacctcg
    15301 gcacgttcac gtccgccgac aagatgttcc ggctggaggg cgcgatgacg tccagcccgc
    15361 gcaaggtgga gatggcgcgg cggatcttca gcgagttcgt ggacgtgccc gcgctgctgc
    15421 gcgccttgga ggtgcatcgc tccgaggtga tgaccccgct gatgttcgag catcagctgc
    15481 aggagatcgc ccgctctgac cgccggacga tcgtgatgcc cgaagcaacc gacgaccgga
    15541 ttctggagtc ggccgacatc ctgctgcggc gcggagtggc gaagctgatc ctgcttgggg
    15601 atgccctgat gatcaagcag cgcgcgaccc acctcggact cgggctgcac ggcgtcgaag
    15661 tggtcgaccc gaccgacccg gagctggtgg agcggttcgc ggccgagtac gcgcggctgc
    15721 gcgcgcacaa gggcgtgacg ctcgagcagg cgcgcgagcg gctcaccgac ctgtcgtact
    15781 tcgggacgat gatggtgcac ctgggcatgg cggacgggat ggtctccggc gcgatcaaca
    15841 cgaccgcgaa cacgatccga ccctcgctcg aattcatcaa gaccaagccg ggcgtgaccg
    15901 tggtctcggg ctctttcctg atgtgcatgc ccgaccgggt gttggtctac gccgactgtg
    15961 cggtgaaccc cgatccgacc gcagaacagc tcgccgacat cgcgatcagc tcggccgaga
    16021 ctgcagtggc tttcggcatc gaaccccgag tcgcgatgtt gtcctactcg accggcactt
    16081 cgggatcggg cgcggacgtg gataaggtgc gcgaggcgac cgcgatcgtc cgcgaacggc
    16141 gtcccgacct ggcactggag ggtccgatcc agttcgatgc ggcggtcgac ccgatcgtag
    16201 cgaagacgaa gctgccgaat tcaccggtgg ccggtaaggc gacggtgttc atcttccccg
    16261 acctgaacac cggcaacaac acctacaagg cggtgcagcg ctcgtccgga gccgtggcca
    16321 tcgggccggt gctgcagggc ctgaacaagg cggtcaacga cctgtcccgc ggggccctgg
    16381 tcgaggacat cgtcaacacc gtcgcgatca ccgccatcca ggctgggcaa gagtcccccc
    16441 ggaacaactg aggagaacca ctgtgagcac tccgatcctg ctgctgaact gcggctcgag
    16501 ttcgatcaag taccaggtga tcgacgccga cgacgaggac gtgctcgcgt ccggcatcat
    16561 ccagcggatc gggcagcccg agagcacgct cgaccacaag ttcggcgacc agacccgcca
    16621 cgatgaccac ggcttcccga accatgccag ggcgctggcg cacctcgtcc gcgtgttcaa
    16681 cgaggttggt ccgaatctgg cggacgtggt cgcggtcggg caccgcaccg tccatggcgg
    16741 cgaagcgttc cgcgagacgg tggtgatcga cgacgaggtg ctggccaagc tgcgcgaact
    16801 cagcccgctg gcgccgctgc acaacccgcc gggcatcgcg ggcatcgagg cggcccgcga
    16861 ggtactgccg gacgtcccgc acgtggcgat cttcgacacc gcgttcttcg cgaccttgcc
    16921 ggccgaggcg tacacgtacg cgatcgacgc cgagctggcc gctgaacacg gcatccgaaa
    16981 gtacggcttc cacggcacgt cgcacagctt cgtgtccaag aaggtcgccg aatacctcgg
    17041 acgcgacctc ggttcgctga agcagatcgt ctgccacctg ggcaacggtg cgtcgatctc
    17101 ggcgatcgac ggcggcgtcg cggtggagac gtccatggga ctgacgccgc tggccggcct
    17161 ggtgatgggc actcggtccg gcgacgtcga ccccggcctg cacgcgttcc ttggccggac
    17221 actcgggctc gatctgggtg gcgtggacga tctgctgaac aagaagagcg ggatgctcgg
    17281 cctggtcggg gtgaccgatt tccgcgatct cggcgagctg atcgacgccg gggacgccaa
    17341 ggcgcagctg gctatggacg tctatgtgca ccggctcgtg ttctacatcg gcgcctacca
    17401 cgcgctgctc ggcggactgg acgcgctgac gttcaccgcc ggagtgggtg agaacgacga
    17461 cctggtgcga cgcctggtgg tgagccgtct gggagccctc ggcatcgagc tggactcgga
    17521 cgccaaccag gtacgcgcca agcacccccg aacgatctcc accccggctt caaaggtgac
    17581 cgtgctggtc gtcccaacga acgaagagct ggccatggcc cgcgagacca agaccgccat
    17641 cagctgagtc cgtcaggctt gagctgctaa ccggtccctg aggagcgttg tgaggaacga
    17701 gcagcgcgcc gcgttgggcc gctgacaccg aagtgtcggg tccggcgctg accaaccctc
    17761 agtcgttccc gacggcggtc gaggagtgag ttgtccacag cggttgtcca cagtggggat
    17821 aactacacgg gtgtgattag cgtccctggc agccggtcca gtgcgcggtc cgctgtccga
    17881 ccgcggcttc acgtccgcgg tcggcccgct ccgtccgcac aagaactaat tgttcggctg
    17941 caccggccgg gagtagcttc cgacctgagc ggctcacccc tgccggtgtc gttgcgagga
    18001 cgtattgtcc atggacgagg acttcttcac ggcacgggag ggtgagaccc cggccccgat
    18061 tgctgcggtc ccggtgactt gtcggcaagc gtctctaccg taggcagtac gccaccttgt
    18121 tcccggga
//
LOCUS       DAOPRQ010000053        17757 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_2674_length_17757_cov_12.886058, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000053 DAOPRQ010000000
VERSION     DAOPRQ010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 17757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17757
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2674_length_17757_cov_12.886058"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..222)
                     /locus_tag="PKJ61_08060"
     CDS             complement(1..222)
                     /locus_tag="PKJ61_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002527944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3073 domain-containing protein"
                     /protein_id="HOC13819.1"
                     /translation="MGRGRAKAKQTKVARDLKYRSYSTDFGSLERELRGGESDDPVAE
                     LAIEVEDDQYGDLADRYSDDEDEPFRRIG"
     gene            complement(335..1396)
                     /gene="purM"
                     /locus_tag="PKJ61_08065"
     CDS             complement(335..1396)
                     /gene="purM"
                     /locus_tag="PKJ61_08065"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HOC13820.1"
                     /translation="MAEQSAYAAAGVDIEAGDRAVELMKASVAASHRPEVLGGLGGFA
                     GFFDASALAAYRRPVLATSTDGVGTKVAIARAMGVYDTIGYDLIGMLVDDLVVTGAEP
                     LFVTDYIACGKVFPERIAAIVGGIAAACKDAGAALLGGETAEHPGLMSPEDFDVAGAT
                     TGVVEYDSILGASRVQPGDVVLALASSGLHSNGYSLVRHVFASASWGLERVVPELGRT
                     LGEELLTPTRVYARDLLDIIRATEVHSISHITGGGLANNLVRVLPDGIGVDLDRVTWT
                     PPAIFDLVQSVGGISQPDIEATLNMGVGMALVLPESSVASAQAVASAAGIDSWVMGAA
                     VAEPDAPARVTLHGAHAAG"
     gene            complement(1389..2843)
                     /gene="purF"
                     /locus_tag="PKJ61_08070"
     CDS             complement(1389..2843)
                     /gene="purF"
                     /locus_tag="PKJ61_08070"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002545370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOC13821.1"
                     /translation="MLSPGDDWTDDPGPRDACGVFGVWAPGEEVAKLTYFGLYALQHR
                     GQESAGMAVSNGERIMVFKDMGLVSQVFDEPTLSSLIGFVSVGHCRYSTTGSSTWSNA
                     QPTFRGTTDGGLALAHNGNLTNTDELEAWLATSVEESDVPHKDRMDSTSDTALVTALM
                     AVYADEGLKAAALKVLPRLEGAFCLVFSNERTLFAARDPQGIHPLVLGRLERGWVVAS
                     ETAALDIIGATFIREVEPGELVSIDESGLSSSRFATADPKGCLFEYVYLARPDTLISG
                     RRVHAVRVEIGRTLAMEAPVDADLVIPTPESGTPSAIGYAEASGIPYGLGLVKNSYVG
                     RTFIQPSQTIRQLGIRLKLNPIRDVIEGKRLVVVDDSIVRGNTQRALVKMLREAGAAE
                     VHVRISSPPVQWPCFYGIDFATRAELIAPGLTVDEICRSLGADSLGYISLEGLTASTG
                     IAGKRLCRACFDGDYPVPIPAVRARQMGLERKFG"
     gene            complement(2883..3776)
                     /locus_tag="PKJ61_08075"
     CDS             complement(2883..3776)
                     /locus_tag="PKJ61_08075"
                     /inference="COORDINATES: protein motif:HMM:NF021221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HOC13822.1"
                     /translation="MSANDQEPTVVEPAEAELVAPVEESVESVEPTLVEPIEDAEVIE
                     DVTPTEPVLISSQLAPKLTLDDTEPGAAAVVELDAVEATEPAAPEAPAEHSLDLPDAE
                     APSLPEPPTFDEPAYVAPTFPDQPSYSPPYAQPVPPQAPYAQPTYQQAPYAQPNQQPN
                     SAYAPNSAYAPNPAYGLQPQYPQSSLNPSEEQTWATAAHWSALVASFVGLGFLGPLLI
                     LLIQGQKSPRVRANAVESLNFEITYVIGMIASVLLMLVVVGFVTIIVFPLLWLIFRIV
                     ASVQTANGQDYRYPLNIRLVK"
     gene            3898..5367
                     /locus_tag="PKJ61_08080"
     CDS             3898..5367
                     /locus_tag="PKJ61_08080"
                     /EC_number="1.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015072012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinic semialdehyde dehydrogenase"
                     /protein_id="HOC13823.1"
                     /translation="MQFEVIRPFDGSVYGTIPATPAAEVPAAIARARQAQVAWADVPP
                     SERAQVFTRLARLVFAERDRILDVIQAETGKSRLSAFEEVMDAGRGVRVFASRSPRLL
                     RPVRRGGAIPLLTRTFEHHRPVGVVGIINPWNYPFNLPATDSAQALLAGNAVIFKPDS
                     QTPFTALLLAELLEAAGLPDGLFQVLPGSGAELGPAMVAGVDHLMFTGSTSVGRQLAV
                     ACAERLIGFSGELGGKNPLLVLADANVPAAVAGTVRAAFANTGQVCVGIERAYVVGEV
                     YDEFVAQLVRSTVGLRLGAGYDWQTDVGSLASAKQFAAVTAHVEDAIAKGATVLAGGR
                     ARPDLGPLMYEPTILTDVTEEMTLCREETFGPVLALYRVESEEAAIRAANDTRYGLNA
                     SIWSAHGEAIAGRIKAGTVNINEGFAATWGSHAAPMGGMGESGMGRRHGSHGLFKYTE
                     PQTVSRQRLHPIAPPQNVGNETYANVMTTAIRLLNRLGG"
     gene            5369..6151
                     /locus_tag="PKJ61_08085"
     CDS             5369..6151
                     /locus_tag="PKJ61_08085"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOC13824.1"
                     /translation="MQLDGARFLITGGGSGLGRQLALQAAARGAHVEVWDLDAAAAAA
                     TTEITGGTSQRVDVTDTAAVDAAAAAAGAIDVLVNCAGIVSGKSLLDASEAAIRRTFE
                     VNTLAGYWTTRAFLPGMLDRDRGTIVTLASAAGLVGVARQTDYSASKWAAIGFTESLR
                     AELRASGSQVSTLVVAPYYIDTGMFAGVRTRVPLLLPILKEAKVATQILDAIASGRQQ
                     LVLPPLARVVPALRVLPVRAFDVVMDLFGINHTMDHFIGRGR"
     gene            6321..7649
                     /locus_tag="PKJ61_08090"
     CDS             6321..7649
                     /locus_tag="PKJ61_08090"
                     /inference="COORDINATES: protein motif:HMM:NF014741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HOC13825.1"
                     /translation="MDEQVGVVPVELALAQIGAALGSIDPNRARLAPEARLHAARTAR
                     SLTGRLDALASLLLAEADAVHASERTVGTPTSTWLAIDQNLTKREAAGALHRASELAA
                     HPVLGEAAVAGRVSTGQVRAINTMLDGLAAQLNDQQQAQAETLLVSLADRMDSDALGK
                     AAAQVLAKVAPADADELLETKLQREAEAAHRNRSLRFWRQGGSVRFEGSLPKLAGEQF
                     ITLLDAHSEALRRTAVEARDPLHVNATPEQRRADALASLLHDAATARPKPGVGAAKVI
                     VKLDYDQLRKGAAGAGVVGDGVQLTAGELRRACCDAEIIPVVLRAGSEVLDVGRSRRL
                     VTPAIRAALIARDGGCVFPGCTAPPSACEAHHIIPWWLGGLTALSNLVLLCHHHHGLV
                     EPAKYGLRDQWQVRISEADRLPEFAPPSRHPQAGEWLRHAQHRPRERIAA"
     gene            complement(8003..9340)
                     /gene="gdhA"
                     /locus_tag="PKJ61_08095"
     CDS             complement(8003..9340)
                     /gene="gdhA"
                     /locus_tag="PKJ61_08095"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HOC13826.1"
                     /translation="MPVAVRPAFESVLRRNPGEPEFHQAVHEVLASIVPVLGEHPHYL
                     EDGILERLVEPERQIIFRVPWVDDNGRVHVNRGYRVQFSSVLGPYKGGLRFHPSVNSS
                     IIKFLGFEQIFKNALTAQGIGGGKGGSDFDPHGRSDAEIMRFCQSFMNELYRHLGEHT
                     DVPAGDIGVGAREVGFLFGQYRKITNRHESGMFTGKGTGWGGAEVRTEATGYGTVYFA
                     QEMLAVTGKSIAGRRVLVSGSGNVAIYAIEKAQQLDALPITASDSSGYIVDEAGIDLD
                     LLKEVKEVERLRISAYADRRPSARFVPGARVWEVPGEIALPCATQNELDGADAETLIR
                     NGVLAVAEGANMPSLPEAVDAFQSAGVLFAPGKAANAGGVATSALEMSQNAARQRWTF
                     ADSERKLHHIVQNMHEAAYVASERYGSPGDYVVGANCAAFVKVADAMIAQGII"
     gene            9475..9612
                     /locus_tag="PKJ61_08100"
     CDS             9475..9612
                     /locus_tag="PKJ61_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13827.1"
                     /translation="MVEPTGRLHERPIAVSELAEAEGLAFLNSLRGWCPADLAVRAER
                     A"
     gene            complement(9593..10102)
                     /locus_tag="PKJ61_08105"
     CDS             complement(9593..10102)
                     /locus_tag="PKJ61_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13828.1"
                     /translation="MTGKRFIFATGHVEPGMTKPTIALRRSQAQLIVAELNRLNPENL
                     PIIWGSDLASSKLSPSGNTAYDVFRSAGFTDPLGNSYKVKKKNKLTYAKKLVNAQYFT
                     LNNFKAQPDKNKRYKLGAHLDYILIKTPGKKATVAQWKQVLYLDKLGKFSGVIPSDHN
                     LVAVTLALP"
     gene            complement(10099..10743)
                     /locus_tag="PKJ61_08110"
     CDS             complement(10099..10743)
                     /locus_tag="PKJ61_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13829.1"
                     /translation="MTAANRLIALCVAGAMMAVPWSPARADAAPLPEAGRFVAPAAAG
                     SANLVVATFNVRNANWSSQRPTNSREKSWDQRRPVVVSQILGEGVDVLGVQETTAADV
                     TANGPQYLDLLAALGEPYAITSESRYDGGEYCYTDYVEDADGNYVQNPDGSYVEQQIC
                     EWEPEASGDVRIIYKKSRLVLDAPRTDQGVLKLDDRDNRNGSARFMAWARFRDL"
     gene            complement(10854..11897)
                     /locus_tag="PKJ61_08115"
     CDS             complement(10854..11897)
                     /locus_tag="PKJ61_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13830.1"
                     /translation="MKRLLVSLAALALTTVPFWAPTQTPAQAAPAPAAANSSSLVVAT
                     YNVRCANCSKHPVNKREKRWNTRAAAIASTIAGEGIDLIGVQESSSGVLWKGGPAQFE
                     DLVNRLGAPYALTNGARYNCVRSTTPSSCDPAYQGASGDSRIIYNTNKLDLLAQGSLA
                     LDDRKPGNGSARFLAWAKFSERATGKQFFFGTAHLEPGMSKAKIGKRAGQANAAIAEL
                     NRVSEGLPVIWGSDLASSKLSHSGNTAYDAFMAAGYTDPLGNSYKARTANAGTYAASM
                     INEEYFTLNNFAKKPKKYAAYALGAHLDYILLKPQMQVLEWKQVLNLKSNGRFAGVIP
                     SDHNLVKVTLVLP"
     gene            complement(12020..12739)
                     /locus_tag="PKJ61_08120"
     CDS             complement(12020..12739)
                     /locus_tag="PKJ61_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016668117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - oxidoreductase activity, acting
                     on NAD(P)H, nitrogenous group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HOC13831.1"
                     /translation="MLPAEFAALVRARHSVRDFRPDPIPDEVLNSILDDARYAPSWSN
                     TRPYIVAVASGERLDRLRTAYVRAFDASLGLQHRKPWAVLRGLLLRRGYPDGDFKAWR
                     GYPAELRERSVKVGKALYAHLGIARGDRAARDANARLNCEFFGAPTVLWLFVHDQMLP
                     FSAHDAGLMLQTLMLSAEANGVSSCPLGVLAVWRHPIDAEFSVPKHYKLVTGLALGYA
                     SDAPVNDFRAEHPPLSVAPSR"
     gene            complement(12817..13659)
                     /locus_tag="PKJ61_08125"
                     /pseudo
     CDS             complement(12817..13659)
                     /locus_tag="PKJ61_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002544370.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
     gene            13867..17742
                     /gene="purL"
                     /locus_tag="PKJ61_08130"
                     /gene_synonym="purI"
     CDS             13867..17742
                     /gene="purL"
                     /locus_tag="PKJ61_08130"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF003672.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HOC13832.1"
                     /translation="MTHQFILTSFPGGNALSEFRADALVRRLREASVPVTAVVANYAH
                     WVASGADLDEATAKTVGELLSYGPAAEPSPAAAQIVVVAPRLGTLSPWASKATDIAHS
                     CGVPIRRVERVTEYHLTTERPLTEADWLTAADLLHDRMTESVFASVNESAALFNERVA
                     EPMAHVDVLGRGRAAIEEANLTFGLALSDDEIDYLVTAFGNLHRNPTDVELTMFAQAN
                     SEHCRHKIFNADFIVDGVPQPDSLFGMIRHTEAVAGHGTVVAYKDNASIMAGGPVQRW
                     APEGADAPSRYVATTDDVHVLMKVETHNHPTAISPFPGAATGAGGEIRDEGATGRGSA
                     PKAGLTGFAVSNLHLPGTDEPWERDPYGAPEHIASPLEIMTAAPLGAAAFNNEFGRPG
                     LGGFFRVFEQTVAGVRRGFHKPIMSAGGLGSISASQTEKISFAPGTLLVQIGGPGMRI
                     GMGGGAASSMAAGANAAELDFDSVQRGNPEIQRRAQEVINHCWKAGADNPILSIHDVG
                     AGGLSNAFPELVDGAGLGARFDLSAVPVEESGLAPKEIWSNESQERYVLAIAPESLDG
                     FAALCERERCPFAVVGVARDDGQLVLAQGASDNADDDRPIDMPMDVLLGKPPRMTRDV
                     RRIVRSEPALDLSGVDLRQAAYAVLRHPSVASKRFLITIADRTVGGLNHRDQMVGPWQ
                     VPVADVAVTLSDFTSLAGQAMATGERMPLASVDAPASGRMAVGEALTNLLAAPVELSG
                     VKLSCNWMAACGEDGEDAALYDTVHAVAMELCPALGISVPVGKDSLSMRTRWADDSGP
                     KQVTSPVSLVVSAFASLKSVEGTWTPQLQPDSTLVLVDLGGGKDRLGGSMLAQVTGQF
                     GDVVPDLDDPSQLVALAAALTELRSQSLVTGYHDRSDGGLWAAACELGFAGSTGLELD
                     VPSIEALFSEELGVVLGVADDDVDRVMTILDARGLGRVSRLVGQTTDDRQMRVSIAGE
                     PLLDEPLRDLAQAWDEVSWRISALRDNPECADEEHAAFGTDSPGLVVAPTFDPSDDIT
                     APYLATGVRPKVAILREQGVNSHVETAFAFHRAGFEVYDVHMTDLQAGRFDLADAIGL
                     VACGGFSYGDTLGAGEGWARSVRFNPKLTDDFSSFFAREDTFGLGICNGCQMFAALAD
                     IIPGADAWPLFTRNRSEQYEARLSQVEVLESSSIFFSGMAGSRIPIAVAHGEGFANFA
                     VRGDLAQVAKAARFVDGDGRPATTYPANPNGSPDGLTAVTTTDGRFTAMMPHPERVAR
                     NAQLSWTTGELNAASPWLRMFRNARVWVG"
BASE COUNT         2801 a         6036 c         6061 g         2859 t
ORIGIN      
        1 tcagccgata cggcggaagg gctcgtcctc gtcgtcggag tagcgatcgg cgaggtcacc
       61 gtactggtcg tcctcaacct cgatggccag ctcggcgacc gggtcgtcgg actcaccacc
      121 ccgcaactcg cgctccagcg acccgaagtc ggtggagtag gaacggtatt tcagatcgcg
      181 agcgactttg gtctgcttcg cttttgcacg gccgcgcccc atatggtcgg gtcagccccc
      241 tcgcctgttc tcagcggtcg gagccgcttc atgtttcaag tgtcgtcgtg aggccaaggg
      301 tacctaatcc gcgcaccctg gcaaagacgg cggctcagcc cgcggcgtga gctccgtgca
      361 gggtgacccg ggccggcgcg tccggctcgg cgacggccgc gcccatcacc caggaatcga
      421 tcccggcagc gctggccacg gcctgcgcgg acgcaacgga cgactccggc agcaccagcg
      481 ccatgccgac gcccatgttc agggtggcct cgatgtcggg ctgcgaaatc ccgcccaccg
      541 actgcaccag atcgaagatc gccggcgggg tccaggtgac ccggtcgagg tcgacgccga
      601 tcccgtccgg cagcacccgg accaggttgt tggccagccc gccgccggtg atgtggctga
      661 tcgaatgcac ctcggtggcg cggatgatgt ccagcaggtc gcgcgcgtac acccgggtgg
      721 gcgtgagtaa ctcttcgccc agcgtccggc cgagttcggg cacaacccgc tccaggcccc
      781 acgaagcgga cgcgaacacg tgccgcacca gcgaataccc gttggagtgc agccccgacg
      841 atgccagcgc cagcaccacg tcccctggct ggacccggct cgcgccgagg atcgagtcgt
      901 actcgaccac gccggtggta gcgccggcca cgtcgaagtc ctctggagac atcaagccgg
      961 gatgctcggc cgtctcgcca cccagcagtg ctgctccggc gtccttgcag gcagccgcga
     1021 tgccgccgac gatcgccgcg atccgctcgg gaaagacctt gccgcaggcg atgtagtcgg
     1081 tgacgaacag cggctcggcg ccggtgacca ccaggtcgtc cacgagcatg ccgatcaggt
     1141 cgtagccgat cgtgtcgtag acgcccatcg ctctcgcgat cgcgaccttg gtgcccaccc
     1201 cgtccgtgga cgtggccagc accggacgcc ggtacgcggc cagcgcggac gcgtcgaaga
     1261 agccggcgaa accacccagc ccgccgagca cctcgggacg gtgggacgcc gcgaccgagg
     1321 ccttcatcag ctcgacggcc cggtcaccgg cctcgatgtc gacgcccgcg gcggcgtacg
     1381 cgctctgctc agccaaactt ccgctccaat cccatctggc gagcccggac agccgggatc
     1441 ggcactggat aatcaccgtc gaaacaggcc cggcaaagcc gcttgccggc gatgccggtc
     1501 gaagcggtga gcccttccag gctgatgtag cccagcgagt ctgcgcccag cgagcggcag
     1561 atctcgtcga ccgtcagccc gggggcgatc agctcggcgc gggtggcgaa gtcgatcccg
     1621 tagaagcacg gccactgcac cggcggggac gagatgcgca cgtgcacctc ggctgcccca
     1681 gcttcgcgca gcatcttgac cagggcgcgc tgggtgttgc cgcggacgat cgagtcgtcc
     1741 accacgacca gccgcttgcc ctcgatcacg tcgcggatcg ggttcagctt cagccggatg
     1801 ccgagctgcc ggatggtctg ggacggttgg atgaacgtcc ggccgacata ggagttcttc
     1861 accaggccca gcccgtacgg aatgccggac gcctccgcgt agccgatcgc ggacggcgtc
     1921 ccggattcgg gcgtgggaat caccaggtcg gcgtccaccg gggcttccat ggcgagcgtc
     1981 cgaccgatct cgacgcggac ggcgtgtacc ctgcggccag agatcagggt gtccgggcga
     2041 gccaggtaga cgtactcgaa caggcagccc ttggggtcgg ccgtggcgaa ccgcgagctg
     2101 ctcaggccgg actcgtcgat cgagaccagc tcgcccggct caacctcgcg gatgaaggtg
     2161 gcgccaatga tgtccagcgc tgcggtctcg gacgcgacca cccagccgcg ctccaggcgt
     2221 ccgagcacca atgggtggat tccctgcgga tcgcgggctg cgaagagagt gcgctcgttg
     2281 ctgaagacca ggcagaaggc gccttcgagc cgcggcagca ccttcagcgc ggcggctttg
     2341 aggccctcgt ccgcgtagac ggccatcagc gcggtgacca gcgcggtgtc ggacgtggag
     2401 tccatgcggt ccttgtgcgg cacgtccgat tcttcgacgg acgtggccag ccaggcttcg
     2461 agctcgtcgg tgttggtgag gttgccgttg tgcgcgaggg cgagcccccc gtccgtggtg
     2521 ccccggaagg tgggttgggc gttgctccag gtgctcgaac cggtggtcga gtagcggcaa
     2581 tgcccgaccg atacgaagcc gatcagggac gagagggtgg gctcgtcgaa gacctgcgag
     2641 accaggccca tgtccttgaa gaccatgatc cgctcgccgt tactgaccgc catgcccgcg
     2701 gactcctggc cgcggtgctg cagcgcgtac agcccgaagt aggtgagttt ggccacttcc
     2761 tcgcccggcg cccaaacacc gaagacgccg caggcgtccc ttggacccgg gtcgtcagtc
     2821 cagtcgtcgc ccgggctcag cacgggccaa actctacctt cgatcggacg ggctgcccga
     2881 cctcacttca ccaggcggat gttcagcggg tagcggtagt cctggccgtt ggcggtctgg
     2941 acgctagcca cgatccggaa gatcagccac agcagcggga agacgatgat cgtgacgaaa
     3001 ccgaccacga ccagcatcag tagcacgctc gcgatcatcc cgatgacgta ggtgatctcg
     3061 aagttcagcg actcgaccgc gttcgcccgt acccgcgggc tcttctggcc ctggatcagc
     3121 aggatcagca gcgggccgag gaaaccgagg cctacgaagc tggccaccag cgccgaccag
     3181 tgcgccgctg tggcccaggt ctgctcctcg ctcgggttca gcgacgactg cgggtactgc
     3241 ggctggagcc cgtaagccgg gttcggcgca tacgccgagt tcggcgcata cgccgagttc
     3301 ggctgctgat tcggctgcgc gtacggggct tgctggtacg tcggctgggc gtaaggcgcc
     3361 tgcggcggaa ctggctgggc gtacggcgga ctgtagctcg gctggtccgg gaaggtcggc
     3421 gccacgtagg ccggttcgtc gaacgtgggt ggctccggca gcgacggagc ctcggcatcg
     3481 ggcaggtcga gactgtgctc ggccggggcc tccggtgcgg cgggctcggt ggcttccact
     3541 gcgtccagtt cgaccaccgc ggcagcgccc ggttcggtgt cgtcgagcgt gagcttcggc
     3601 gcgagctggc tggagatgag cacgggctcg gtgggggtga cgtcctcgat cacttcagca
     3661 tcctcgatcg gctcaaccaa ggtgggttcc accgactcaa ccgactcctc caccggcgct
     3721 accaactcag cttcagcggg ctcaacgacg gtgggttcct gatcatttgc agacatgctt
     3781 gcagcctatt gcggcgaccc gtccgcggca gcactcccag ggtgcggacg gggtcggacg
     3841 ggtggcgccc ctgacccgaa cctgggaagg cggacgccag tcccctactg tgacggcatg
     3901 cagttcgagg tcatccgtcc gttcgacggc agcgtgtacg gcaccatccc ggccactccg
     3961 gcggcggagg taccggcggc catcgccagg gcccgccagg cccaagtggc ttgggcggac
     4021 gttcccccga gcgagcgcgc ccaggtgttc acccggctgg cccggctggt ctttgccgag
     4081 cgtgaccgga tcctcgacgt catccaggcc gagaccggca agtcccggct gagcgctttc
     4141 gaagaggtga tggacgcggg tcgcggcgtc cgggtctttg ccagcaggtc gccgcggctg
     4201 ctgcgtccgg tgcgccgtgg cggcgcgatc cccctactca cccgcacttt cgagcatcat
     4261 cgtccggtcg gcgtcgtggg catcatcaac ccatggaact acccgttcaa cctgccggcc
     4321 accgattctg cgcaggctct gctcgccggc aacgcggtga tcttcaagcc cgactcgcag
     4381 accccgttca ccgcgctcct gctggccgag ttgctagagg ccgccgggct gccggacgga
     4441 ctgttccagg tgctgcccgg ctcaggtgcc gagctcggac cggcaatggt ggcaggcgtc
     4501 gaccacctga tgttcaccgg ctccaccagc gtcggacgcc agctcgccgt cgcgtgcgcc
     4561 gagcgcctga ttggcttctc gggcgaacta ggtggcaaga atcccctact ggtactcgcg
     4621 gacgcgaacg tgccggcggc cgtggcgggc accgtccgcg cagccttcgc caacaccggc
     4681 caggtctgcg tgggcatcga gcgggcctat gtggtcggcg aggtctacga cgagttcgtg
     4741 gcgcagctcg tccgcagcac cgtgggactg cggctgggcg ccggctacga ctggcagacc
     4801 gacgtcggct cgctcgcctc ggccaagcag ttcgccgcgg tgaccgcgca cgtcgaggac
     4861 gcgatcgcca agggcgcgac cgtgctggcc ggcggccgcg cgcggcccga cctcggaccg
     4921 ctcatgtacg agccgaccat cctcaccgac gtcaccgaag agatgacgct gtgccgcgag
     4981 gagaccttcg gcccggtgct ggcgctctac cgcgtcgagt ccgaggaagc cgccatccgc
     5041 gcggccaacg acacccgcta cggactcaac gccagcatct ggtcggccca cggcgaggcg
     5101 atcgccgggc ggatcaaggc cggcaccgtg aacatcaatg agggcttcgc cgccacctgg
     5161 ggctcgcacg ccgcgccgat gggcggcatg ggcgagtcgg gcatgggccg tcggcacggc
     5221 agtcatggtc tgttcaagta caccgagccg cagacggtca gtcgacagcg gctgcacccg
     5281 atcgcgccgc cgcagaacgt gggcaacgag acctacgcga acgtgatgac cactgcgatc
     5341 cggttgctca accggcttgg aggctgagat gcaactcgat ggagcccggt tcctgatcac
     5401 cggcggcggc agtgggctgg gccgccagct ggcgctgcag gctgcggctc gcggcgcgca
     5461 cgtcgaggtg tgggacctgg acgcggccgc tgccgcagcc accaccgaga tcaccggcgg
     5521 cacgtcccag cgcgtggacg tcacggacac cgcagcggtc gatgccgcgg ccgctgccgc
     5581 cggcgcgatc gacgtgctcg tgaactgcgc ggggatcgtc agcggcaagt cgctgctgga
     5641 cgcgtccgag gctgcgatcc gcaggacgtt cgaggtgaac accctggccg gctattggac
     5701 cacgagggca tttctgcccg gcatgctcga ccgtgatcgc ggcacgatcg tgacgctcgc
     5761 cagcgccgct gggctggtcg gcgtggcccg gcagaccgac tattcggcca gcaagtgggc
     5821 ggcgatcggc ttcaccgagt cgctccgcgc cgaactgcgc gcgtccgggt cgcaggtcag
     5881 cacgctggta gtcgcgccct actacatcga caccggcatg ttcgcagggg tgcggacgcg
     5941 ggtgccgcta ctgctgccga tcctgaagga agccaaagtg gccacccaga tcctggacgc
     6001 gatcgcgtcc ggacggcagc agctggtgct accaccgctg gctcgggtgg tgccggccct
     6061 gcgagtcctg ccggtacggg ccttcgacgt cgtgatggac ctgttcggta tcaaccacac
     6121 catggaccac ttcatcgggc gcggacgctg agctgggctg ccgccaccat catcggcccc
     6181 tgaggaagcc cgcgaggaac gagccggcag tcacggatat ccaccgcgat tcctgatatt
     6241 tgcctgagaa tcctcgggcg gtggccggat agggctgttc tggacgaaaa ctgtcagagc
     6301 cgcccgatta gctggtggtc atggacgagc aggtaggcgt ggtgccagtg gagttggcac
     6361 tggctcagat tggtgctgcc ctgggctcga tcgaccccaa ccgtgctcga ctcgctcccg
     6421 aagcccgtct ccacgcggcc aggactgccc ggagcctgac cggccggttg gatgcgctgg
     6481 cgtcgctatt gctggccgaa gctgatgccg tgcacgcctc ggagcgaacc gtcggtacgc
     6541 ccacttcgac gtggctggcg atcgatcaga acttgaccaa acgtgaagct gccggcgcgt
     6601 tgcaccgcgc cagtgagctg gctgcgcacc cggtgctggg cgaggccgct gtcgctggca
     6661 gagtgagcac tgggcaggta cgggcgatca acaccatgct cgatggtctg gctgctcagc
     6721 tgaacgacca gcagcaggcg caggccgaga cgttgctggt gagcctggcc gaccgtatgg
     6781 attcggatgc gttgggcaag gccgccgcgc aggtgttggc gaaggtcgcc ccggctgatg
     6841 ctgacgagct actcgaaacc aagctgcagc gtgaggccga agccgcgcac cgtaaccggt
     6901 cgctgcggtt ctggcggcag ggcgggtcgg tgcggttcga aggctccctg cccaagctgg
     6961 ccggtgagca gttcatcacc ctgctcgacg cccacagcga agcgctgcgc cgcaccgcgg
     7021 tcgaggcccg cgacccgctg cacgtgaacg ccactccgga acagcgccgc gccgacgcac
     7081 tggccagcct gctgcacgac gcggcaaccg cccgacccaa acccggtgtg ggtgcggcga
     7141 aggtgatcgt gaaactcgac tacgaccagt tgcgcaaggg tgctgctggc gccggggtgg
     7201 tcggtgacgg tgttcagctg acggctggtg agctgcgccg agcctgctgt gacgccgaga
     7261 tcatcccggt ggtgctgcgt gccggtagcg aggtgttgga tgtgggccgc tcccggcggc
     7321 tggtcacacc ggcgatacgg gcggcgctga tcgcccggga tggcggctgt gtgttccctg
     7381 ggtgtaccgc gccaccatcg gcgtgtgaag cccaccacat catcccgtgg tggctgggtg
     7441 gtttgaccgc tctttcgaat ctggtcctgc tgtgtcacca ccaccatggc ctggtcgaac
     7501 cagccaagta cggactgcgg gatcagtggc aggttcggat cagcgaggct gaccggctac
     7561 cagagttcgc cccaccatct cggcatccac aagcaggtga gtggctgcgc cacgcccagc
     7621 atcggccacg agagcgaata gcggcttgac ccgaccggcc cgcagtttgg gccggacgcg
     7681 aagcctgccc acaggccgcc gcgtcggctg gtcgagcccg gccaagccaa gcccagccaa
     7741 gcccggggct gaacggtggt tgaacttggc gagggccctc accaggacgc cggccgaacc
     7801 cccacgttgg gtcacggcgt gggtggcgtc acggcgttgg gtgtctccca cgacgttggg
     7861 tgcctgtcgg cacgacgttg ggtggtggcg tcggctcgat tagccgaagc ggagcgcgac
     7921 caaaagaccc aggttcggtg gtcgagctgt cgagatcgcg tggtctcgac aggctcgacc
     7981 tgccgagcaa gtgcatagcg actcagatga tgccttgggc aatcatggcg tccgcaacct
     8041 tcacgaaggc ggcgcagttg gcgcctacga cgtagtcgcc ggggctgccg taacgttcgg
     8101 acgcgacata ggccgcctcg tgcatgttct gcacgatgtg gtgcagcttg cgttcggagt
     8161 cggcgaaggt ccagcgctgg cgggccgcgt tctggctcat ctccagcgcc gaggtggcca
     8221 caccaccggc gttggcggcc ttgcccggcg cgaacagcac tccagccgac tggaacgcgt
     8281 ccacggcctc cggcagcgaa ggcatgttcg cgccctcagc tactgccagc acgccgttgc
     8341 ggatcagtgt ctccgcgtcc gctccgtcca actcgttctg ggtagcgcac ggcagcgcga
     8401 tctcacccgg cacctcccac acccgtgctc ccggaacgaa gcgggcggac ggacggcgat
     8461 cggcgtaggc gctgatgcgc agccgctcga cctccttgac ctccttgagc aggtcgaggt
     8521 cgatgccggc ctcgtccacg atgtagcccg aagagtcgga cgcggtgatc ggcaaggcgt
     8581 ccagctgctg cgccttctcg atcgcgtaga tcgcgacatt gcccgacccg gagaccagca
     8641 cgcggcgtcc cgcgatcgac ttgccggtga ccgcgagcat ctcctgggcg aagtagaccg
     8701 tgccgtaacc cgtggcctcg gtgcgcacct cggcaccacc ccagccggtg cccttgccgg
     8761 tgaacatgcc ggactcgtgc cggttggtga tcttgcggta ctggccgaaa aggaagccga
     8821 cctcgcgggc cccgacgccg atgtcgccgg cgggaacgtc cgtgtgctcg ccgaggtggc
     8881 ggtagagctc gttcatgaac gactggcaga accgcatgat ctcggcgtcg ctgcggccat
     8941 gcgggtcgaa atcggaaccg cccttgccgc cgccgatgcc ctgcgcggtg agtgcgttct
     9001 tgaagatctg ctcgaagccg aggaacttga tgatcgagga gttcaccgaa gggtggaacc
     9061 gcaggccgcc cttgtagggg ccgagcaccg aggagaactg cacccggtag ccgcggttca
     9121 cgtgcacccg gccgttgtcg tcgacccacg gcacccggaa gatgatctgc cgctccggct
     9181 cgaccaaccg ctccaggatc ccgtcctcca ggtagtgcgg gtgctcgccc aggaccggca
     9241 cgatcgacgc cagcacctcg tgcacggcct ggtggaactc cggctcgcca gggttccggc
     9301 gcagcaccga ctcgaaggcg ggtcggacgg caaccggcag ggtatccgga tcgagcgtgt
     9361 gggtggcgac ggacaagttg gggcctctcg cggtggcgga cgctgaaggg ccgcgtcctg
     9421 ttgagccgtg ggcaattctc gcagtttccc gggccggcgc tggtatgcgc ttggatggtg
     9481 gaacccaccg gacggctgca cgagcgcccg atcgcggtgt ccgagttggc cgaagccgag
     9541 gggctggcgt tcctgaacag cctgcgaggc tggtgcccgg ccgacctggc cgtcagggca
     9601 gagcgagcgt gaccgcgacc aggttgtggt cggacgggat cactcctgag aacttgccca
     9661 acttgtcgag gtaaagcacc tgcttccact gcgcgaccgt ggccttcttg cccggggtct
     9721 tgatcaggat gtagtccagg tgggcgccca gcttgtagcg cttgttcttg tccggctggg
     9781 ccttgaagtt gttcagcgtg aagtactggg cgttgaccag ctttttcgca taggtcagct
     9841 tgttcttctt cttcaccttg tacgagttgc ccagcggatc cgtgaaacca gcgctgcgga
     9901 acacgtcgta ggcggtgttg ccggacggtg agagcttgct gctggccaaa tcgctgcccc
     9961 agatgatcgg caggttctcc gggttgagcc ggttgagttc ggccacgatc agttgcgcct
    10021 gggagcggcg cagcgcgatc gtcggcttgg tcatccccgg ctcgacgtgt ccggtggcga
    10081 agatgaaccg cttgccggtc acagatcgcg gaaccgcgcc caggccatga accgggcact
    10141 gccgttgcgg ttgtcgcgat cgtcgagctt caacacgccc tggtcggtgc gcggcgcatc
    10201 caggaccagc cgcgacttct tgtagatgat ccgcacgtca ccggacgcct ccggctccca
    10261 ctcgcagatc tgctgctcga cgtaactgcc gtccgggttc tgcacgtagt tgccgtccgc
    10321 gtcctcgacg tagtcggtgt agcagtactc gccaccgtcg tagcggctct cgctggtgat
    10381 ggcgtacggc tcgccgaggg ccgcgagtag atcgaggtac tgcgggccgt tggcggtcac
    10441 gtccgccgcg gtggtctcct ggacgccgag cacgtcgacg ccctcaccga ggatctggct
    10501 gaccaccacc gggcggcgct ggtcccagct cttctcccgg ctgttcgtcg gacgctgact
    10561 cgaccagttg gcgttgcgga cattgaacgt cgccacgacc agattggccg atccggccgc
    10621 ggccggtgcg acaaagcgtc cggcttcggg aaggggcgct gcgtccgctc gggccggcga
    10681 ccagggcacg gccatcatcg cgccggctac gcacaatgcg atcaggcggt tggctgctgt
    10741 cattgcttcc cccgttctgc catgacgaag gtagcggcgt tgccggccgg cgggaatacg
    10801 cagaactgta cgaatctgga cggggcgacg gttccttttc gttcaggggt gcgtcagggc
    10861 agaaccagcg tcaccttcac caggttgtgg tcggacggga tcacgccggc aaacctgccg
    10921 ttgctcttca ggttcagtac ctgcttccac tccagcacct gcatctgcgg cttcaacagg
    10981 atgtagtcca ggtgggcgcc gagtgcgtag gccgcgtact tcttcggctt cttggcgaag
    11041 ttgttgagag tgaagtactc ctcgttgatc atcgaggccg cgtaggtgcc cgcgttggcc
    11101 gtccgggcct tgtaggagtt gcccagcgga tcggtgtagc cggcggccat gaacgcgtcg
    11161 taggcggtat tgcccgagtg cgacagcttg ctgctggcca ggtcgctgcc ccagatcacc
    11221 ggcaggcctt cggaaacccg gttcagctcg gcgatggccg cgttcgcctg gccggcgcgc
    11281 ttgccgatct tcgccttgct catgcccggc tccaggtgag ccgtgccgaa gaagaactgc
    11341 tttccggtgg cacgttcgga gaacttcgcc caggccagga agcgtgcgct gccgttgccg
    11401 ggcttgcggt cgtccagggc cagcgagccc tgcgcaagca ggtccagctt gttggtgttg
    11461 tagatgatcc ggctgtctcc ggacgcgccc tggtaggccg gatcgcaact gctcggggtg
    11521 gtggagcgga cgcagttgta gcgggcaccg ttggtaagcg cgtagggggc gccgaggcga
    11581 ttgaccagat cctcgaactg ggccgggccg cccttccaaa gcacgccgga ggaggactcc
    11641 tgcacgccga tcagatcgat gccctcgcct gcgatggtgg acgcaatcgc ggcggcgcga
    11701 gtgttccagc gcttctctcg cttgttcacc gggtgcttgg agcagttggc gcagcgcacg
    11761 ttgtacgtgg ccaccaccag actggacgag ttcgccgcgg ctggggcggg agcggcctgt
    11821 gcgggggtct gcgttggtgc ccagaacggc accgtggtca gcgccagcgc agccagcgaa
    11881 accagtaatc gcttcatatg tgggggtact tccttgggag gggtggacgc ctgatcctta
    11941 aaaactctca ggcgggactc agaataaccc cagattgaag cgttgcgaaa cccattgcca
    12001 tcgaggcacg gccggggtgt cagcgactcg gcgcgaccga aagtggcgga tgctcagcgc
    12061 ggaaatcgtt cacgggcgcg tccgaggcgt agcccagggc gagtccggtg accagcttgt
    12121 agtgcttcgg caccgaaaac tccgcgtcga tcggatgccg ccaaaccgcg agcacaccga
    12181 gcgggcacga gctgacaccg ttcgcctcgg ccgaaagcat gagcgtctgg agcatgagcc
    12241 cggcgtcatg agccgagaac gggagcatct ggtcgtgcac gaacagccac aacacggtcg
    12301 gcgccccgaa gaattcacag ttcagtcgcg cattcgcgtc ccgcgccgcc cggtcgccgc
    12361 gagcgatccc caagtgcgcg tagagcgcct tgcccacctt cacgctgcgt tcgcgcagtt
    12421 ccgccggata cccccgccac gccttgaaat caccatccgg atagccccgg cgcagcagta
    12481 ggccacgcag gacggcccac ggtttgcggt gttgcaggcc aagggacgca tcgaaggcgc
    12541 ggacgtaggc agtccgcagc cggtcgagcc gctcgccgga ggcgaccgcg acgatgtacg
    12601 gacgggtatt gctccagctc ggcgcgtacc gagcatcatc aaggatggag ttcaacacct
    12661 cgtccgggat cggatcggga cgaaagtcgc gaacgctatg gcgggcgcgc accagcgcgg
    12721 cgaattcagc gggcagcacg gccgcagtct attcgcgcgc gctcggactg ccagcagatc
    12781 cggacgcgga ctacgtggcc ctgaggaacc tcagaccgcc aggtggcgtc ccaccaggcg
    12841 gcggtaggcc tccaggtagc ggtcccgggt ggcctgcacg atcgcgtccg gcaacggcgg
    12901 tggcggagtg tccgaagcct tgtcccagcc tgattccttg gcgagccagt cccggacgaa
    12961 ctgcttgtcg aagctcggca ggctttgtcc cggctgccag acggacgcgt cccagaagcg
    13021 cgacgaatcg ggcgtgagca cttcgtcggc cagcacaagg gtgccgtcgg cacgcaggcc
    13081 gaactcgaac ttggtgtcgg ccaggatgac gctgcgctca gccgccagtt cggctgcctt
    13141 cgagtagatg ctcagggtag cggtgcgcag gcgctcggcc agctcagcac cgtgcaactc
    13201 gacgatcgtg gcaaaatcca cgttctcgtc gtgctcaccg agcggggcct tgatcgccgg
    13261 ggtgaagatc ggctccggca gcttggctcc atccgccagt ccagccggca gcgggatacc
    13321 gcacaccgtc ccggagctct ggtactccga ccagccagag ccggtcaggt agccgcgggc
    13381 cacacattcc accggcagca tgtcgaggga ctccacgacc accgcgcggc cggccacctc
    13441 gtcgggaacc tcggtcgaaa cgatgtgatt ggggaagtcg agttgctcga accaccacag
    13501 gctgagctgg gtcagcacga cccccttgtc ggggatcggc gtggccagca catggtcgta
    13561 cgccgaaatc gcgtcggtgg caaccatcag gatccggccg tccgaccagc ggtagaggcg
    13621 acgcaccttg ccggcatgca gcagcgggag atcaagcacg gcctcagcgt atcggcatgg
    13681 ctcggcgacc cttcgtcagg atcatggatc attcggcccg ttggtcggcc cggtcgtcca
    13741 gatcggcgcc cgtcgttgtc caaggcaggg cggggcttct cgccaggaga cgcgcggacg
    13801 gccgggcggc gccgggccgt tagccttgag gggtcgcccg tccagccatc cggaggttcg
    13861 cccgtcgtga cgcaccagtt catcctgacc agcttccccg gcggcaacgc cctctccgag
    13921 ttccgggcgg acgcgctcgt ccggcgcctt cgggaagcga gcgttccggt gaccgcagtc
    13981 gtggccaact acgcccactg ggtggcgagc ggggccgatt tggacgaagc caccgcgaag
    14041 accgtgggcg aactgctcag ctacggtccc gcggccgagc cgtcgccagc ggccgctcag
    14101 atcgtggtgg tcgcgccccg tctcggcacg ctgtcgccct gggcgtcgaa ggccaccgac
    14161 atcgcgcaca gctgcggagt accgatccgc cgggtcgagc gggtcaccga ataccacctc
    14221 accaccgagc gtccgctcac cgaggctgac tggctcaccg ccgccgatct gctgcacgac
    14281 cgcatgaccg aatctgtctt cgcctccgtg aacgagtccg ccgcgctgtt caacgagcgc
    14341 gtagccgagc cgatggccca cgtcgacgtg ctcggacgcg gccgcgccgc catcgaagaa
    14401 gccaacctca ccttcggact cgccctcagt gacgacgaga tcgactatct ggtcaccgcg
    14461 ttcggcaacc tgcaccgtaa ccccaccgac gttgaattga ccatgttcgc ccaggccaac
    14521 tccgagcact gccggcacaa gatcttcaac gccgacttca tcgtggacgg cgtcccgcag
    14581 cccgactcgc tgttcggcat gatccggcac accgaggcgg tcgccggtca cggcacagtc
    14641 gtcgcctaca aggacaacgc ctcgatcatg gccggcggtc cggtccagcg ctgggccccc
    14701 gaaggcgcgg acgcacccag ccgctacgtc gccaccaccg acgacgtgca cgtgctgatg
    14761 aaggtggaga cccataacca cccgaccgcg atcagcccct tccccggagc cgccaccggc
    14821 gccggcgggg aaatccgcga cgagggtgcc accggccgcg gctcggcccc gaaggccggc
    14881 ctgaccggct tcgccgtctc gaacctgcac ctgcccggca ccgacgagcc ctgggagcgc
    14941 gacccctacg gcgcgcccga gcacatcgcg tcgccgctgg aaatcatgac cgcagccccg
    15001 ctgggtgcgg ccgcgttcaa caacgagttc ggccgtccgg gcctcggcgg cttcttccgg
    15061 gtgttcgagc agaccgtcgc cggcgtccgc cgcggcttcc acaagccgat catgagcgcc
    15121 gggggcctgg gctcgatcag cgcgtcccag accgagaaga tcagcttcgc gcccggcacg
    15181 ctgttggtgc agatcggcgg gccgggcatg cggatcggca tgggcggcgg cgcggcctcg
    15241 tccatggccg ccggtgcgaa cgctgccgaa ctcgacttcg actccgtcca gcgcggcaat
    15301 ccggagatcc agcggcgcgc gcaggaggtc atcaaccact gctggaaagc cggcgccgac
    15361 aacccgatcc tctcgatcca cgacgtgggg gcgggcgggc tctccaacgc cttccccgag
    15421 ttggtggacg gcgcgggcct cggcgcgcgc ttcgacctga gcgcagtgcc ggtcgaggaa
    15481 tcgggcctgg cgccgaagga gatctggagc aacgagagcc aagagcgcta cgtgctggcg
    15541 atcgcacccg agtccctgga cggcttcgct gcgctgtgcg agcgtgagcg ctgcccgttc
    15601 gcggtggtgg gggttgcccg cgacgacgga cagttggtgc tggcgcaagg agcgtccgac
    15661 aacgccgacg acgaccgtcc gatcgacatg ccgatggacg tgctgctcgg caaaccgccg
    15721 cggatgaccc gcgacgtgcg ccgcatcgtc cgctccgagc cggccctcga cctgtcggga
    15781 gtcgacctcc ggcaggccgc gtacgcggtg ctgcggcatc cgtccgtggc ttcgaagcgg
    15841 ttcctgatca ccatcgccga ccgcaccgtg ggcgggctca accatcgcga ccagatggtc
    15901 gggccgtggc aggttccggt cgccgatgtc gcggtgactc tctccgactt cacctcgctg
    15961 gccggccaag cgatggccac cggcgagcgg atgccgctgg catcggtgga cgcgcccgcg
    16021 tccggacgga tggcggtcgg cgaagcgctg accaacctcc tcgccgcccc ggtggagctg
    16081 tccggggtga agctgtcctg caactggatg gccgcgtgcg gcgaggacgg cgaggacgcc
    16141 gcgctctacg acaccgtgca cgccgttgcg atggagctgt gcccggcgct cggcatctcg
    16201 gtgcccgtgg gcaaggactc gctctcgatg cgcacccgtt gggcggacga ttccgggccc
    16261 aagcaggtta cctcgccggt ctcgctggtg gtctcggcgt tcgcttcgct gaagtctgtt
    16321 gaaggcacct ggacgccgca gcttcagccc gactccaccc tggtgctggt cgacctcggc
    16381 ggcggaaagg accgcctcgg cggctcgatg ctggcccagg tcaccggcca gttcggcgac
    16441 gtggtgcccg atctggacga cccgtcccag ctcgtcgccc tagccgcggc gctcaccgaa
    16501 ttacgtagtc aaagcctggt taccggctac cacgaccgct cggacggcgg actgtgggcg
    16561 gcggcctgcg agctcggctt cgccggttcg accggcctcg aactcgacgt ccccagcatc
    16621 gaagcgctgt tcagcgaaga gcttggcgtg gttctcggcg tcgccgacga cgacgtcgat
    16681 cgggtcatga ccatcctcga cgcccgcggt ctcggccgcg tcagccgcct ggtcggacag
    16741 accaccgacg accgccagat gcgggtcagc atcgccggcg aaccgttgct ggacgaaccc
    16801 ctacgtgatc tcgcccaggc ctgggacgag gtctcctggc gcatctcggc cctgcgcgac
    16861 aaccccgagt gcgccgacga ggagcatgcc gccttcggca ccgactcccc cggcctggtg
    16921 gtcgcaccga ccttcgatcc gtccgacgac atcacggcgc cgtacctcgc gaccggcgtc
    16981 cggccgaagg tggcgatcct gcgcgagcag ggggtgaaca gccacgtgga gaccgcgttc
    17041 gccttccacc gcgccggctt cgaggtctac gacgtgcaca tgaccgacct gcaggccgga
    17101 cgcttcgacc tcgccgacgc gatcggcctg gtggcgtgcg gcgggttctc ctacggcgac
    17161 acgctcggcg cgggtgaggg ctgggcccgc tcggtgcggt tcaacccgaa gctgaccgac
    17221 gacttcagct ccttcttcgc ccgcgaggac accttcgggc tgggcatctg caacggctgc
    17281 cagatgttcg cggctctggc cgacatcatt ccgggcgcgg acgcctggcc gctgttcacc
    17341 cgtaaccgct ccgagcagta cgaggcccgg ctctcccagg tcgaagtgct cgaatcgtcc
    17401 tcgatcttct tcagcggtat ggcgggtagc cggattccga tcgcggtggc gcacggcgag
    17461 ggcttcgcga acttcgccgt ccgcggcgac cttgcacagg tcgccaaggc cgcccgcttc
    17521 gtggacggtg acggccggcc ggcgaccacc tacccggcca acccgaacgg ttcccccgac
    17581 ggactgaccg cggtcaccac caccgacgga cggttcaccg cgatgatgcc gcaccccgag
    17641 cgggtggccc gcaacgcgca attgtcctgg accaccggcg agctgaacgc ggccagcccc
    17701 tggctacgga tgttccgcaa cgcgcgggtc tgggtgggct gaccgggcct cacccct
//
LOCUS       DAOPRQ010000054        17216 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_2790_length_17216_cov_11.666744, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000054 DAOPRQ010000000
VERSION     DAOPRQ010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 17216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17216
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2790_length_17216_cov_11.666744"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..128
                     /gene="xseB"
                     /locus_tag="PKJ61_08135"
     CDS             <1..128
                     /gene="xseB"
                     /locus_tag="PKJ61_08135"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004812011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HOC13833.1"
                     /translation="SVPLSESMKLWERGEELARICQEWLDGAVAKIEAARPKSEE"
     gene            complement(261..689)
                     /locus_tag="PKJ61_08140"
     CDS             complement(261..689)
                     /locus_tag="PKJ61_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOC13834.1"
                     /translation="MALLNPYLNFQGNAREAIEFYHSVFGGTLDISTFGEFQMPGSGP
                     EDQDKVMHAQLTTPSGFTLMASDVPPGMPFSDGSRITVSLSGNETDELTGYFNQLAEG
                     GNVTQPLQAAPWGDSFGQLADRFGTTWLVNIAGPGAESAG"
     gene            complement(865..1545)
                     /locus_tag="PKJ61_08145"
     CDS             complement(865..1545)
                     /locus_tag="PKJ61_08145"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOC13835.1"
                     /translation="MEVAHPNQLGAVYEQLLKRSFPPQELADRDSFLNFVTWGEVLVE
                     RDGELITAAAVGDYSPNTDLLLLEYLAVLPEYRGKGSGSKLLEAAFAQWCELMHPGAF
                     LAEIERPDGHQGTPEYGDPVRRLKFYHKLGLKALALPYYQPALSPELAPVPDLLLGVL
                     IEDESWVQDHRFVEGERLAALLRERNPEPTDVEGPAWQALLDACAGPIELVDLGDYAS
                     VPRSGPIG"
     gene            1582..2205
                     /locus_tag="PKJ61_08150"
     CDS             1582..2205
                     /locus_tag="PKJ61_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13836.1"
                     /translation="MRAETPFGEIALRRRNGVTELVVNGVFAMDSVEVTSELALADAA
                     GRPPGRVLVGGLGLGYTTARLLDAGADLVRVVELAAPLVEWARAGATRQLGRVAADPR
                     VELVVGDVADEIAGAEPGWDSILLDVDNGPSFLIHDHNQRVYSAAFLNHCLHLLRPGG
                     RLVIWCEAESPELATTLRRMAEVQLVTVPVNREGRSFDYALYLASPR"
     gene            complement(2267..2755)
                     /locus_tag="PKJ61_08155"
     CDS             complement(2267..2755)
                     /locus_tag="PKJ61_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13837.1"
                     /translation="MKGNGALQVIFAFFLGLVVVGFVGIGVNTFYPEPSNLDYDAKQY
                     GQWRLVTGIILLVLATLILVISLTLPAVQVVISNGLLLGGVFTMIYAVGMTVSADQSI
                     WRFVVVAVALIVTIAIGYAKFVRRTAAPVAAAVDGVPADVAARLAHLERRFEALRRAL
                     GE"
     gene            2816..3202
                     /locus_tag="PKJ61_08160"
     CDS             2816..3202
                     /locus_tag="PKJ61_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020387810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CpaE"
                     /protein_id="HOC13838.1"
                     /translation="MISHEQGRQLRLLVAWEPASGDRFVIPGTDLDEVFVIAEMTIEA
                     QDVPGGQIIKFNGTTEWALDSIAAEKVLWLPREDQLRTLLSYAFVRLEVVGDGYAVEL
                     TDGTRHVDIDAERAYARAVIAWLGRR"
     gene            complement(3343..4686)
                     /locus_tag="PKJ61_08165"
     CDS             complement(3343..4686)
                     /locus_tag="PKJ61_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOC13839.1"
                     /translation="MPRSSTRSTTTAASRTVLALKTYVIDTSVLLSDPHALRNFAEHG
                     VVVPVVVITELEAKRHHPELGYFARAALRFLDDLRVHYGRLDAPIPVNDLDGTLRVEL
                     NHTDTDVLPPGFRLGDNDSRILAVALNFKAEGHDVVLVSKDLPMRVKASAVGLVAEEY
                     RHELTPDSGWTGMVEVDVPTATIEQLYTEGAAIVPEALDLPCHTGVVLHGSASSALGR
                     VTTSGSVRRIQDRDAFGIHGRSAEQRVALDLLLDPDVGIVSLGGRAGTGKSALALCAG
                     LEAVLERRLHSKVIVFRPLFAVGGQELGYLPGTEGEKMSPWGQAVFDTLGSVTSKNVI
                     DEVLAQDLLEVLPLTHIRGRSLHDAFVIVDEAQSLERNVLLTVLSRIGQNSRVVLTHD
                     VAQRDNLRVGRHDGVVAVVERLKGHPLFAHVTLTRSERSPIAALVTEMLADVPLG"
     gene            complement(4905..5708)
                     /gene="uppS"
                     /locus_tag="PKJ61_08170"
     CDS             complement(4905..5708)
                     /gene="uppS"
                     /locus_tag="PKJ61_08170"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014604536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HOC13840.1"
                     /translation="MSLGLRVREAVNRLQPLGFLYSAYETQLLAQLDRSALPQHVAVL
                     ADGNRRWARANAPEQPLVVGYQAGADKLRQFVDWCADLGIGVVTLWVLSTENLTRSES
                     DEVAPLLNVIEHLVAQLATQYRVKVVGDLNLLPEQMAQNLHDAEANGERHSGIHVNVA
                     IAYGGRHELRDAVRSLLATEAAKGTTLDQLADTLEIDHISEHLYTAGQPDPDLVIRTS
                     GEQRMSGFLIWQSAHSEFYFCEALWPDFRKVDFIRALRSYAQRERRYGR"
     gene            5918..6565
                     /locus_tag="PKJ61_08175"
     CDS             5918..6565
                     /locus_tag="PKJ61_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015069779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HOC13841.1"
                     /translation="MTAEILPDKPKLRGWLHLGMAVAVVFGGAALLVLAPGVAGKAGS
                     AVWLVGAIVLFGMSAIYHIGSWSPPVKATLRRLDHANIFVFIAATYTPLALVLLPRSD
                     AIALLTLIWAVAVVGVSVRVFWQNAPRWLDVACYLGLGWAGIGWLPAFWVNGGPLIVI
                     LIGVGGLIYSLGALVYARKWPDPSPKWFGFHEIFHACTIVAALCHFAAIALAVLR"
     assembly_gap    6627..6726
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6879..8891)
                     /locus_tag="PKJ61_08180"
     CDS             complement(6879..8891)
                     /locus_tag="PKJ61_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002533780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOC13842.1"
                     /translation="MNRLAEATSPYLRMHADNPIDWWEWGPEALAEAAQRDVPILLSV
                     GYGACHWCHVMAHESFSDPQLAELVNPNFVAIKVDREERPDIDAVYMAATQALTGSGG
                     WPMTVFLTPDGRPFFAGTYYPPERAGGMPSFSEVLSAISDAWRERRDEADQSASAITA
                     HLAGALTPQRATTDRFDVWGVLDRLGTEFDAIHGGFGTAPKFPAPLLIDALGVKGEPG
                     SLDVAQRSLDAMARGGIHDQIGGGFARYSVDPAWVVPHFEKMLYDNALLLGSYVRGWR
                     RVPDHDPVLRWQFERTVRGIVGWLERELRTDEGGFASSLDADSLDIHGRQHEGIYYCW
                     TPELLTDALGEADGQWAAEVFHVTRLGSFEHGLSTLQLRGNPDPERLAAVSARLLAER
                     QQRFAPARDAKVIAAWNGWAIDSLVWAALVFDEPAWLELARNAAECLWRVHWVEGRLR
                     RSSLGGVASQAPGMAEDYGAVALGFARLAGALGDAEWLVRAHTLVASAQELFGAEDGG
                     FYDSAVDAEALIVRPRDVSDNPTPSGTSALVAALRLVGLLADDATLIARADAAAETVR
                     GLLEQTPRFAGSALVDALVADDARTGLRPAVAVVVGDPLDEAARATWRMAPIGTAIVA
                     GAPGTQGFAHHFEERAEPGVYVCRGTVCFPPTSDLAELRTALWSRV"
     gene            complement(8917..9105)
                     /locus_tag="PKJ61_08185"
     CDS             complement(8917..9105)
                     /locus_tag="PKJ61_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13843.1"
                     /translation="MVLLDLGSSVVTPSWLPLVLVIVLLGLVAFLYFNMRGNINRIDF
                     EEQPRPASPVSPDEQPTP"
     gene            complement(9087..9971)
                     /gene="mca"
                     /locus_tag="PKJ61_08190"
     CDS             complement(9087..9971)
                     /gene="mca"
                     /locus_tag="PKJ61_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020385525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010126 - mycothiol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycothiol conjugate amidase Mca"
                     /protein_id="HOC13844.1"
                     /translation="MGMSLRLLNVHAHPDDESSKGAATTAYYVAQGVEVMVATCTGGE
                     RGSILNPKMESPENWANLAALRNAEMARAAEILGIEQVWLGYVDSGMDEPLPEGCFAL
                     QDVDQAAGPLVKVIREFKPQVLTTYDENGGYPHPDHIMCHRISMAAVAAAADPAAWPE
                     HGEPWQVAKVYYDMSLHRGRFAALDAAMHKAGLERPYSDWLSQWGEDTGASARLTTFI
                     PCSDYFEARNAALRAHATQIDPDGHWFAVPLEIQQVGWPTEDYQLVKSTVPVRLPESD
                     LFAGLRTEDSLGTWCYSI"
     gene            10024..10425
                     /locus_tag="PKJ61_08195"
     CDS             10024..10425
                     /locus_tag="PKJ61_08195"
                     /inference="COORDINATES: protein motif:HMM:NF025520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4307 domain-containing protein"
                     /protein_id="HOC13845.1"
                     /translation="MTDPDATARLARRYPARRTTRAWIPLAILLAVLGAIWLVWAGSY
                     GATGTVTARVDAFEVRSDTQIDVTVTIDRPDPGKAAECLLYSQAVTYDRVGEAKVRVE
                     PGGSSLTTLNIELRTFKRATTAAVENCRALG"
     gene            10452..10955
                     /gene="greA"
                     /locus_tag="PKJ61_08200"
     CDS             10452..10955
                     /gene="greA"
                     /locus_tag="PKJ61_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002514137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOC13846.1"
                     /translation="MTEQPTTWLTQDAFDRLTEELAYLKTDGRRLVSAKIAQAREEGD
                     LSENGGYHAAREEQGHQEARIRQLTAMLENAQVGEAPADSDEVTPGVAVTIYYDDDPD
                     DTDTFLLGSRELMGLDESVDIEVYSPQSPLGAAILGHKVGETVTYRAPAGHLIEVTVV
                     EVKPFTA"
     gene            complement(11053..11433)
                     /locus_tag="PKJ61_08205"
     CDS             complement(11053..11433)
                     /locus_tag="PKJ61_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 domain-containing protein"
                     /protein_id="HOC13847.1"
                     /translation="MTAKLYQAPAYCPVCGDALITTRLGCRGCGSELAGEFGHCDFCA
                     LDERESELLRVFLASRGNLREVEKHLGVSYPTARARFTALLTKLGLAGEAEPVAMTRD
                     QVLSEVATGVLSPAEAAELLAGLG"
     gene            complement(11430..12347)
                     /locus_tag="PKJ61_08210"
     CDS             complement(11430..12347)
                     /locus_tag="PKJ61_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13848.1"
                     /translation="MSTPDLGPILEDLAAGRIDASEAARRIDALKATAAPEPPETKPA
                     EPTNEELAAEELGDSPRRQYSPHARDVFAQPEGSPDAPTGRRRPTGSKGVDRISVRAV
                     GRRVRIVGDPSVATASAEGPHVLRRNGAVLEITSDGELGPSLDGFSILRPPRSLDDIR
                     ALGLGKELLLRINPSIIVDVEVTAGHLTCNDVPYLGKVRVTAGGADLTGVAEAHDVLV
                     QAGSATLKGAITTGRSRVRVESGQLSVDLDDHSNVTVRGEAQLGKVSWSGAHTGQVDE
                     VVLGNGSARLDVGVVMGWANIKAGNEVAQ"
     gene            complement(12393..13460)
                     /locus_tag="PKJ61_08215"
     CDS             complement(12393..13460)
                     /locus_tag="PKJ61_08215"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOC13849.1"
                     /translation="MPGPRIPQGVVTAAEWSWRLLVIAAVLALIWWLLQYFSGVAVPL
                     AVAMLLSALLSPLNARLRRFGWPRVLASIGSLLAMVLVIGGVFLAIGAQVARELPELI
                     DQSVKALATLVDWLAAGPLHIDQAQLDGYLAQFTTWVNESRAELASIAAKAGVGVGHF
                     LAGLAIALIATFFFLASGREIWSSVVKLLPVHYQAPTLRAADNGWASLVAYMRAQVLV
                     ALVDAIGMLAGALILRLPMAWALFALTFVTAFIPVVGAVLSGAVACLLALVTHGPVSA
                     LIMLGITIAVVQTEGHFLQPILLGRAVSLHPLAVLIGLAVGATLAGIVGALLVIPVLA
                     FSVAFIRGLDPARFKPVRLQE"
     assembly_gap    13635..13734
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            13794..14468
                     /locus_tag="PKJ61_08220"
     CDS             13794..14468
                     /locus_tag="PKJ61_08220"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016669384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOC13850.1"
                     /translation="MRTPLPQLMAGDWAEALAPVESQIVAMGDFLRAEIAAGRGYLPP
                     GPNVLWAFQRPLADVRVLIVGQDPYPTPGHAVGLSFSVAPDVRPLPRSLANIYTELNT
                     DLGIRPPESGDLTPWADQGVLLLNRVLTVRPGAAASHRGKGWEAVTERAIDALVARGG
                     PLVAILWGRDAQSLAPRLAGVPQIASAHPSPLSAHAGFFGSRPFSRANAALVAQGGEP
                     IDWRLA"
     gene            14506..15129
                     /gene="msrA"
                     /locus_tag="PKJ61_08225"
     CDS             14506..15129
                     /gene="msrA"
                     /locus_tag="PKJ61_08225"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HOC13851.1"
                     /translation="MFTKSTMVAPEAALPGRERGLLTLPAFHEVLGTDIAAAPDGAEV
                     AYFALGCFWGAEKLFWNTDGVLTTAVGYQGGYTPNATYEEVCSGRTGHAEAVKVVFDP
                     KRTSYAKLLKVFLENHDPTQGMRQGNDLGTQYRSAIYFTDAEQEAAARAAIADYQPKL
                     SASGYGAITTEVVPATPFYFAERYHQQYLEKNPNGYCPIHATGVTCG"
     gene            15324..15599
                     /locus_tag="PKJ61_08230"
     CDS             15324..15599
                     /locus_tag="PKJ61_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HOC13852.1"
                     /translation="MCRNIHTLHNFEPAATDAEVHAAALQYVRKIAGTTKPSKANQAA
                     FDEAVAAIAHATEHLLEHLTTSAPPKNRDVEAAKARARSASRYAVPA"
     gene            complement(15682..16098)
                     /locus_tag="PKJ61_08235"
     CDS             complement(15682..16098)
                     /locus_tag="PKJ61_08235"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HOC13853.1"
                     /translation="MNEQSQGDTIVNPVDTTVTVSRSVSQPIKQFWKLLVTREGAEAL
                     LGPGGELGDKGDSWHAADGTYGVIRSYHPLEQIRFSWHAAEEAPKTIVEVDLAAAGEA
                     ETTVEIRHEHIPVYFDAAAITQRWESALDKLAAAAE"
     gene            16752..>17216
                     /locus_tag="PKJ61_08240"
     CDS             16752..>17216
                     /locus_tag="PKJ61_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="subunit A of antiporter complex involved in
                     resistance to high concentrations of Na+, K+, Li+ and/or
                     alkali; Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit A"
                     /protein_id="HOC13854.1"
                     /translation="MLMTLISAHLVIAVVAPILTRVFGQRVFWVAALAPAISFAWLLS
                     LAPDVLAGVPLVEKTDWIPALGVSVNFHLGLLQWVLSLIVTGIGALVLAYCRWYFIAD
                     APQRRVVGLLTAFCAAMIGLVTADDLIAIYVFWEATTVFSYLLIGHDPTRRAN"
BASE COUNT         2835 a         5865 c         5683 g         2633 t
ORIGIN      
        1 gcagcgtgcc gttgtcggag tccatgaagc tgtgggaacg cggcgaggaa ctcgcgagga
       61 tctgccagga gtggctggac ggcgccgtcg caaagatcga ggcggcccgg ccgaagtccg
      121 aggagtaagc gggctgggcg cagcgggcgg ttgctaccct tcgacacgct cagcgcggac
      181 cgaacacgag cagccgaggt cgctcgcggc tgcctcaaat taaggcaggc tcaggtcgac
      241 ggtccggata gccaggcaga tcagccggct gactccgcgc ccggcccggc gatgttcacc
      301 aaccaagtgg tgccgaagcg atcggccagt tgcccgaagg agtcgcccca cggggcagcc
      361 tgcagcggct gggtcacgtt gcccccctcg gcgagctggt tgaagtagcc ggtcaactca
      421 tcggtctcgt tgccgctgag cgacacggtg attcggctgc cgtcgctgaa cggcatcccc
      481 ggcggcacgt ccgaggccat cagggtgaat ccgctgggag tggtgagttg ggcgtgcatt
      541 accttgtcct gatcctccgg gccggaccca ggcatctgga actcgccgaa ggtgctgatg
      601 tcgagcgtgc cgccgaatac cgagtgatag aactcgatgg cctcgcgcgc gttgccttgg
      661 aagttcaggt aggggttcag cagggccatg tcgcctccaa tttgccgttt ccgacaattc
      721 cgcaggacgc cggtaggcgc gcggccttcg cacctgacca gaagtgcccg ctccggttgc
      781 gcgccgtgga ggatcttgca ccgaactcgg tgcaagatcc tccacgctgc ctgcgctacg
      841 gacgcccaag cgagtggttg agagtcagcc gatgggccca gatcgcggca cgctggcgta
      901 gtcgcccagg tcgaccagtt cgatcggccc ggcacaggca tcgagcagcg cctgccaggc
      961 cggcccctcc acatcggtcg gttctggatt ccgctcgcgc agtagggcgg ccagccgctc
     1021 cccttcgacg aaccgatggt cctggaccca tgattcgtcc tcaatcagca cgccgagcag
     1081 cagatccggt accggcgcca attcgggcga cagcgccggc tgatagtagg gcagcgccag
     1141 cgccttcagc ccgagcttgt ggtaaaactt cagccgccgc accgggtcac cgtattcggg
     1201 ggtgccttgg tgcccgtccg ggcgttcgat ctcggcaagg aacgctcccg gatgcatcaa
     1261 ctcgcaccat tgcgcgaacg cagcctcgag cagcttcgag cccgacccct tgccgcggta
     1321 ttcaggcagc accgccaggt actccagcag cagcaggtcg gtgttcgggg agtaatcgcc
     1381 gacggccgcg gccgtgatca gttcaccgtc ccgctcgacc agtacctcgc cccaggtgac
     1441 gaagttgagg aacgagtcac ggtccgcaag ttcctgcggc ggaaaggaac gtttcagcag
     1501 ctgttcgtag acagcgccga gctggttcgg atgagctacc tccacgccgc gaccctagcc
     1561 gtctgcggca ggattggggg catgagagcc gagactccat tcggggagat cgccctgcgg
     1621 cggcggaacg gcgtcaccga gttggtcgtc aacggcgtgt tcgccatgga ttcggtcgaa
     1681 gtcacctccg aactcgcgct ggcggacgcc gccggacggc ctcccggccg cgttctggtg
     1741 ggcgggctcg gcctgggcta caccaccgcc cgcctattgg acgcgggcgc ggacctcgtc
     1801 cgtgtggtcg aacttgccgc gccgctggtc gagtgggccc gagccggcgc caccaggcag
     1861 ttgggacgcg tcgcggccga tccgcgagtg gagttggtgg tcggcgacgt cgctgacgag
     1921 atcgccggcg ccgagccggg ctgggactcg atcctgctcg atgtcgacaa cggcccgagc
     1981 ttcctgatcc acgaccacaa ccagcgggtc tactccgcag ccttcctcaa ccactgccta
     2041 cacctgctgc gccccggcgg acggctggtg atctggtgcg aagccgagag ccctgagctg
     2101 gcgacgacgc tgcggcgaat ggcggaagtg cagctggtca ccgtcccggt gaaccgggag
     2161 ggccgcagct tcgactacgc gctctacctc gcaagcccga gatgaggggt tgcgaggctg
     2221 gcggcttcga atcgtcgcga acctcaagga tcgccagtcg gcgccactac tcgccgagcg
     2281 cgcggcggag ggcttcgaag cggcgttcaa ggtgtgccag ccgggcggcg acgtccgccg
     2341 ggacgccgtc aaccgcggcc gcgacaggcg cagccgtccg ccggacgaac ttcgcatagc
     2401 cgatggctat ggtcacgatc agcgccaccg cgaccaccac gaagcgccag atgctctggt
     2461 cggcggacac ggtcatgccc accgcgtaga tcatcgtgaa cactccaccc agcagcagtc
     2521 cgttggaaat caccacctgc accgctggca gggtgagcga gatcaccagg atcagggtcg
     2581 ccagcaccag cagaataatg ccggtgacca gccgccactg gccgtactgc ttggcgtcgt
     2641 aatcgaggtt ggaaggctcg ggatagaacg tgttcacgcc gatgccgacg aaaccgacca
     2701 ccaccagacc aagaaagaag gcgaagatca cttgcagcgc accgttgccc ttcatgattc
     2761 aagcctagca acgaccggtt gagcggacgg gtagcgtcgc ccgtggaggt gcgtgatgat
     2821 cagccacgag cagggccggc aactgaggct gctggtcgca tgggagccgg ccagcggtga
     2881 ccgcttcgtc atcccgggca ccgatctgga cgaggtcttc gtgatcgccg aaatgaccat
     2941 cgaggcccag gatgtgccgg gcggtcagat catcaagttc aacggcacca ccgagtgggc
     3001 gctggacagc atcgccgcgg agaaggtgct ctggctaccc cgagaggacc agctgcggac
     3061 gctgctgagc tacgccttcg tccgcctcga agtggtcggg gacggctacg cggtagagct
     3121 caccgacggc acccggcacg tcgacatcga tgccgagcgg gcctatgccc gtgcagtgat
     3181 cgcttggctg ggccgccgct gaacggtggc tgagcttgac gaagcccggc tgagcctgac
     3241 gcccacgatg gctgagcctg atgaagcctc ggcccctcgt caggctcagg gaccgtcgac
     3301 ggacccagcg gtcgttgacg gatcagggcc gttggccggc cctcagccca ggggcacgtc
     3361 cgccagcatc tcggtgacca gcgcggcgat cggcgaacgc tccgagcggg tcagggtgac
     3421 gtgtgcgaac aacggatgcc ccttgagccg ctcgaccaca gcgaccacgc cgtcgtgccg
     3481 gcccacgcgc aggttgtccc gctgggcgac gtcgtgggtg agcacgacgc gggaattctg
     3541 gccgatccgg ctgagcacgg tgagcagcac gttccgctcc agcgattggg cctcgtcgac
     3601 gatcacgaag gcgtcgtgca gcgagcgtcc gcgaatgtgg gtcagtggca gcacctccag
     3661 caggtcctgc gccagcacct cgtcgatcac gttcttcgaa gtcaccgagc cgagcgtgtc
     3721 gaacactgcc tggccccagg gcgacatctt ctccccctcg gtgccgggca ggtagccgag
     3781 ctcctggccg ccgaccgcga acagcggacg gaacacgatc accttcgaat gcaagcgccg
     3841 ctccagcacc gcctccagcc cggcgcacag cgccagcgcc gacttgccgg tgccggcccg
     3901 accgcccaac gacacgatgc ccacgtccgg gtcgagcagg agatcgagcg ccacccgctg
     3961 ctcggcagag cggccatgga tgccgaaggc gtcccggtcc tggatccgcc gtaccgagcc
     4021 ggacgtcgtg acgcgtccga gcgcggacga agccgagccg tgcagcacca cgccggtgtg
     4081 gcagggcagg tcgagcgcct cggggacgat cgcggcgcct tcggtgtaga gctgctcgat
     4141 cgtcgcggtc ggcacgtcga cctcgaccat gcccgtccaa ccggagtcgg gggtgagctc
     4201 gtggcggtac tcctcagcca ccagcccgac cgcagacgcc ttcacccgca tcggcaggtc
     4261 cttgctgacc aggaccacgt catggccctc ggccttgaag ttgagcgcca ccgcgaggat
     4321 ccgggagtcg ttgtcgccca gccggaagcc gggcgggagc acgtcggtgt cggtgtgatt
     4381 cagctcgacc cgcagggtgc cgtccaggtc gttgaccgga atcggggcgt ccagacggcc
     4441 atagtgcacc cgcaggtcgt ccaggaaccg cagcgcggcc cgggcaaagt agccaagttc
     4501 aggatggtgc cgcttcgcct ccaactcggt gatgaccact accggaacca ccacgccgtg
     4561 ctcggcgaag ttgcgcaggg cgtgcggatc gctgagaagc accgaggtgt cgatcacgta
     4621 ggtcttcagt gccagcaccg tcctggacgc agcggtggtc gtggaacggg tggatgaacg
     4681 aggcatgtcc gtcctgtccg agccgaacca ggcgtccggc tcacctagtg gtcagccgag
     4741 taggtccgag ctgggagctg gtcccaggtc tgcgcgatgc atctggtgcc tctcgtcgga
     4801 cgccaggcct gcgtccgtgg tctgagagta agcccggcct tcccgcgtga cttcgcgaca
     4861 cgcccgcgtc cgggggtcga tacgcaagct ttcccgggtc cgcgtcagcg gccgtagcga
     4921 cgctcccgct gggcatagga gcgcagtgcg cgaatgaagt caaccttgcg gaagtcgggc
     4981 cagagcgcct cacagaagta gaactcgctg tgggccgatt gccagatcag gaagccggac
     5041 atccgttgct cacccgaggt gcgaatgacc aggtcaggat caggctggcc ggcggtgtag
     5101 aggtgctcgg agatgtggtc gatctctagg gtgtcggcca attggtccag cgtcgtgccc
     5161 ttggccgcct cggtggccag cagggagcgg acggcatcgc gcaactcgtg gcgtccgccg
     5221 taggcgatgg cgacgttgac gtggatgccg ctgtggcgct cgccgttggc ctcggcgtcg
     5281 tgcaggttct gggccatctg ctcgggcagc aggttgaggt cgcccaccac tttgacccgg
     5341 tactgcgtag ccaactgggc gaccaggtgt tcgatcacgt tcagcagcgg cgcgacctcg
     5401 tcggactcgg accgggtcag gttctcggtg gagagcaccc agagggtgac cactccgatg
     5461 ccgaggtcgg cgcaccagtc gacgaactgg cgcagcttgt ccgccccggc ctggtacccc
     5521 accaccagcg gctgttccgg cgcgttggcg cgggcccagc gccggttccc gtccgcgaga
     5581 acggcgacgt gctgcggcag ggcggaacga tcgagctgag cgagcagctg ggtctcgtag
     5641 gccgagtaca ggaagccgag cggctgcagg cgattaaccg cttcgcgtac gcgcagccct
     5701 agcgacatag gtgcacccta tcccccaacc gccagcggtc ggtcgtacgg cgtgggcgag
     5761 cttccggaca aaatgcccgg tcgatccgcg tttcgaggcg aatcggtggt ctagcgcgcg
     5821 aaacgcgtgc aaatcgtcac gaccccaccg gattgactac gattgatcac gcaacctacg
     5881 ctggcgtaag ttactgaacc gtaagttagg tgctgccgtg accgctgaga tcctgcccga
     5941 caagccgaaa ctgcgtggct ggctgcacct cggcatggcc gtggcagtgg tcttcggcgg
     6001 cgctgcgctg ctcgtcctgg cgcccggcgt ggcgggtaag gccggctctg cggtgtggct
     6061 ggtgggcgcg atcgtgctgt tcgggatgag cgcgatctac cacatcggca gctggtcgcc
     6121 cccggtgaag gcaactttgc gccggctcga ccacgccaac atcttcgtct tcatcgccgc
     6181 cacctacacc ccgctcgccc tggtgctcct gccacgctcg gacgcgatcg cgctgctcac
     6241 cctgatctgg gccgtcgccg tcgtgggcgt gagtgtgcgg gtgttctggc agaacgcacc
     6301 ccgctggctc gatgtcgcct gctatctggg ccttggctgg gccggcatcg gctggctgcc
     6361 cgcgttctgg gtcaacggtg gaccgctgat cgtgatcctg atcggcgtcg gcggcctcat
     6421 ctacagtctc ggtgccctcg tctacgcccg gaagtggccc gacccatcgc cgaaatggtt
     6481 cggcttccac gagatcttcc acgcctgcac gatcgtggcc gcgctctgcc acttcgcggc
     6541 gatcgcgctg gctgtgctgc gctgagccgc ctcgtacacc cgtccctcgt acacccgccc
     6601 tagcacacct gcccctcgca cacccgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6721 nnnnnnacac ccgccctagc acacctgccc ctcgcacacc cgaccgagcc cgtctctcgc
     6781 tccgcaaact ggacagttgc ggccgaagac gggacagaac tgtccagctt gcgggggcgg
     6841 ggggggttcg ggagttggcg cgggtgcgcg gcgcttggct agacgcggct ccacagggcc
     6901 gtgcgcagct cggcgaggtc gctggtcggc gggaaacaga ctgtgccgcg gcagacatag
     6961 acgcccggct cggcacgctc ttcgaagtgg tgcgcgaagc cctgggtccc cggcgccccg
     7021 gcgacgatcg cggtgccgat cggcgccatc cgccaagtgg cccgcgccgc ttcatccaac
     7081 gggtccccca caaccaccgc gaccgccgga cgcaggcccg tccgcgcatc gtccgccacc
     7141 agcgcgtcca ccagggccga gccggcgaac cggggggtct gctccagcag cccgcggacg
     7201 gtttcggcag cggcgtcggc gcgcgcgatc aaggtcgcgt cgtcggccag caggccgacc
     7261 agccggagcg cggcgaccag tgcgctggtt ccggacggcg tcgggttgtc gctgacgtcc
     7321 cgcggacgca cgatcagcgc ttcagcatca acggccgagt cgtagaagcc accgtcttca
     7381 gcaccgaaca gttcctgcgc cgaagctacg agagtgtgtg cacgcaccaa ccactcggca
     7441 tccccgagcg ccccggccag ccgggcgaaa ccgagcgcga ccgcgccata gtcctcagcc
     7501 atccccggcg cctgcgaagc gaccccgccg agcgaactgc gccgcaacct gccctcgacc
     7561 cagtgcaccc gccacaggca ttcagccgca ttgcgagcca gttcgagcca cgccggctcg
     7621 tcgaagacca gcgccgccca gaccagcgaa tcgatcgccc atccgttcca ggccgcgatc
     7681 accttcgcgt cgcgggccgg ggcgaaacgc tgctggcgct cggccagcag cctcgccgac
     7741 acggcagcca agcgttccgg gtcgggattg ccgcggagct gcagggtcga aaggccgtgc
     7801 tcgaacgagc cgagcctggt gacgtggaag acctcggcgg cccactgccc gtccgcttca
     7861 ccgagcgcat cagtcagcag ctcaggggtc cagcagtagt agatgccttc gtgctggcgg
     7921 ccgtggatgt ccaggctgtc ggcatcgagc gaggatgcga acccgccctc gtcggtgcgc
     7981 aactcgcgct caagccagcc gacgatgccg cgcaccgtcc gctcaaactg ccagcgcagc
     8041 accggatcgt ggtcgggcac gcgtcgccaa ccgcgcacgt agctgcccag cagcagcgcg
     8101 ttgtcgtaga gcatcttctc gaaatgcggc accacccagg cgggatcgac gctgtagcgg
     8161 gcgaagccgc caccgatctg atcgtgaatg ccgccacgcg ccatcgcgtc caacgaccgc
     8221 tgggcgacgt cgagcgaacc tggttcgccc ttcaccccga gcgcgtcgat cagcagcggg
     8281 gcggggaact tgggggcagt gccgaagccg ccgtggatgg cgtcgaactc ggtgccgagg
     8341 cgatccagca caccccagac gtcgaaccga tcggtggtgg cccgctgggg cgtcaaggcc
     8401 ccggccaggt gcgcggtgat ggcggacgcg ctctggtcag cctcgtcccg gcgctcgcgc
     8461 caagcgtccg agatcgcgga aagtacttca ctgaaggacg gcatcccgcc ggctcgctcg
     8521 ggcgggtagt acgtcccagc gaagaacgga cgcccgtccg gagtgaggaa gaccgtcatc
     8581 ggccaacccc cggagccggt gagtgcctgc gtggcggcca tgtacaccgc gtcgatgtcc
     8641 ggacgctctt cgcggtcgac cttgatggcc acgaagttgg ggttcaccaa ctcggcgagt
     8701 tgcggatctg agaaggattc gtgtgccatc acgtggcacc agtggcaggc cccgtagccc
     8761 acgctcagca ggatcggcac gtcgcgctgc gccgcctcag ccagcgcctc gggcccccac
     8821 tcccaccagt cgatcggatt gtctgcgtgc atccgcagat agggcgaggt ggcttcggct
     8881 aggcgattca cacgcggcag tttaagccaa gcgcgcttag ggcgtcggct gctcgtccgg
     8941 cgataccggg gaagccggac gcggctgctc ctcaaagtcg atgcggttga tgttgccgcg
     9001 catgttgaag tacaggaacg ccaccaggcc gagcaggacg atcaccagca ccagcggcag
     9061 ccaggacggc gtgaccacgc tcgaacctag atcgagtagc accatgtgcc cagactatcc
     9121 tcggtgcgca gtccggcgaa caggtccgac tccggcagtc tcaccgggac ggtcgacttc
     9181 accagctgat agtcctcggt gggccacccg acctgctgga tctccagggg caccgcgaac
     9241 cagtgcccgt ccgggtcgat ctgggtggcg tgggcacgca gggcggcatt gcgcgcttcg
     9301 aagtagtccg agcatgggat gaacgtggtc aggcgggccg aagcgccggt gtcctcgccc
     9361 cactgcgaca gccagtccga gtacgggcgc tccagacctg ccttgtgcat ggctgcgtcc
     9421 agtgccgcga accggccgcg gtgcagcgac atgtcgtagt agaccttggc cacctgccag
     9481 ggttcgccgt gctcgggcca cgcggccggg tcggcggccg cggctaccgc cgccatcgaa
     9541 atccggtggc acatgatgtg gtcggggtgc gggtagccgc cgttctcgtc ataggtggtc
     9601 agcacctgcg gcttgaactc gcgaatcacc ttgaccagcg gcccggcggc ctgatcgacg
     9661 tcctgcaggg cgaagcagcc ctcgggcaac ggctcgtcca tgccggaatc gacgtagccg
     9721 agccagacct gctcgatgcc gaggatctcg gcggccctgg ccatctccgc gttgcggagc
     9781 gcggccaggt tcgcccagtt ctccgggctc tccatcttcg ggttgaggat cgagccacgt
     9841 tcgccgccgg tgcaggtggc caccatcact tccacgccct gcgcgacgta gtaggcggtg
     9901 gtcgccgcgc ccttgctgga ttcgtcgtcc gggtgggcgt gcacgttcag gagccgcaat
     9961 gacatgccca tattgtgggg atccgagccg ggttcgccca acccggcagc cagaatggac
    10021 gccatgaccg acccggacgc caccgcgcgg ctcgctcgcc gctacccagc gcggagaacc
    10081 acccgtgcct ggattccgct cgccatcctc ctggccgtgc tgggagcgat ctggctggtc
    10141 tgggccggca gctacggggc gaccggcacg gtcaccgcgc gagtggacgc gttcgaagtg
    10201 cgctcggaca cccagatcga cgtcaccgtg acgatcgacc gtcccgaccc cggcaaggcg
    10261 gccgaatgcc tgctctactc ccaggccgtg acctacgacc gggtcggcga ggcgaaggtg
    10321 cgggtcgagc ccggcggctc gtccctgacc acgctgaaca tcgaactgcg gacgttcaag
    10381 cgcgcaacca ccgcagcggt agagaactgc cgcgccttgg gctgagcccg gaatgagcta
    10441 acctccccgg tatgaccgag caacccacta cctggctgac ccaagacgcc ttcgaccgcc
    10501 tcaccgagga acttgcgtac ctcaagaccg acggccgccg gctggtgtcg gcgaagatcg
    10561 cacaggcccg cgaggagggc gacctcagcg agaacggcgg ctaccacgcg gcccgcgagg
    10621 agcagggaca tcaggaggct cgcatccggc agctgaccgc gatgctcgag aacgctcagg
    10681 ttggtgaggc gccggcagac tccgacgagg tcaccccggg tgtcgcggtc accatctact
    10741 acgacgatga tccggacgac accgacacct tcctgctcgg ctcgcgtgag ttgatggggc
    10801 tcgacgagtc ggtcgacatc gaggtttaca gcccacagtc tccccttggc gcggccattc
    10861 tcggccacaa ggtcggcgag accgtcacct accgggcgcc cgctggccac ctgatcgagg
    10921 tgaccgtcgt cgaggtgaag cccttcaccg cctgagccgc aacggtggct gagccgcaac
    10981 ggtggctgag ctcacgaatc cccgcatcac cgagggccag ccctcggtgg cagtgccacc
    11041 gggatcgtct gctcagccga gccccgcgag cagctcagcg gcctcagccg gcgacaacac
    11101 gccggtggcg acctcgctga gcacctggtc gcgggtcatc gccacgggct cggcctcgcc
    11161 cgcaagaccg agcttcgtca gcagtgcggt gaagcgcgcc cgcgcggtcg ggtaggagac
    11221 gccgagatgc ttctccacct cgcgcagatt gccgcgcgag gccaggaata cccgcagcag
    11281 ctcgctctcc cgctcgtcca gcgcacagaa gtcacagtgg ccgaactcgc cggccaactc
    11341 cgatccgcag ccgcggcaac cgagccgggt ggtgatcagc gcatctccac agaccggaca
    11401 gtacgccggc gcctggtaca gcttggcggt cactgcgcca cctcattgcc ggccttgatg
    11461 ttcgcccagc ccatcaccac gcccacatcg agtcgagcgg agccgttgcc gagcacgact
    11521 tcgtccacct ggccggtgtg ggcaccgctc cagctcacct tgccgagctg cgcctcccct
    11581 cggacggtga cattcgagtg gtcgtcgagg tcgacgctca gctgtcccga ctcaacccgc
    11641 acccgggagc gtccggtggt gatcgcgccc ttcagcgtcg ccgaaccggc ctgcaccagc
    11701 acgtcgtgcg cctcggccac cccggttagg tccgcacctc cggcggtcac ccgcaccttg
    11761 ccgaggtacg gcacgtcgtt gcaggtgaga tgaccggcgg tcacctcgac atcaacgatg
    11821 attgacgggt tgatccgcag aagcagttcc ttgcccaggc cgagcgcgcg gatgtcgtcc
    11881 agacttcgcg ggggacgcag gatgctgaac ccgtccaggc tcggaccaag ctcgccgtcg
    11941 ctggtgatct ccagcaccgc gccgttgcgt cgcagcacgt gcggtccctc cgccgacgcc
    12001 gtggccacgg acgggtcacc gacgatccgc accctgcgtc cgaccgcgcg gacggagatc
    12061 cggtcgacgc ccttgctgcc ggtcggacgc cgccgtccag tgggtgcgtc cggcgaaccc
    12121 tccggttggg cgaacacgtc gcgagcgtgc ggcgagtact ggcggcgcgg cgagtcgccg
    12181 agttcttccg cggcgagttc ctcgttggtc ggctcggccg gcttggtttc cggtggctca
    12241 ggagccgcgg tcgctttcag cgcgtcgatt cggcgcgccg cctcggatgc gtcgatccgt
    12301 ccggcggcga gatcctcaag gatgggacca agatcaggag tgctcatagc gcaatagtag
    12361 gtcgcacttt ccaatcagga aaggttctct ttctactctt gcaggcgaac cggtttgaag
    12421 cgggcggggt cgagcccgcg gatgaacgcg accgagaacg ccagcaccgg gatcaccagc
    12481 agcgcgccga cgatgccggc gagcgtggcg ccgaccgcca gcccgatcag cacagccagc
    12541 ggatgcagcg agaccgcacg tccgagcagg atcggctgca ggaagtgacc ctcggtctgc
    12601 accaccgcga tcgtgatgcc gagcatgatc agggcgctga cggggccgtg cgtgaccagc
    12661 gccagcaggc aggccaccgc gcccgagagc accgcgccga ccaccgggat gaacgcagtc
    12721 acgaaggtca gggcgaacag cgcccaggcc atcggcagcc gcaggatcag cgcgccggcc
    12781 aacatgccga tcgcgtccac cagggccacc agcacctggg cgcgcatgta ggcgaccagc
    12841 gaggcccagc cgttgtccgc agcgcgcagc gtaggcgcct ggtagtggac cggcaacagc
    12901 ttcaccacag agctccagat ctcacgtccg gacgcgagga agaaaaaagt tgcgatcagg
    12961 gcgatcgcca ggcctgccaa gaagtgcccg acgcctactc cggctttggc tgcgatcgag
    13021 gccaactcag ccctggattc atttacccag gtggtgaact gggccagata gccgtccaac
    13081 tgcgcctggt cgatgtgcag cggcccagct gccagccagt cgaccagcgt cgccaacgcc
    13141 ttcaccgact ggtcgatcag ctccggcaac tccctggcca cctgggctcc gatcgcgagg
    13201 aacacgccgc cgatcaccag caccatcgcc agtaacgaac cgatggacgc gagcacccgc
    13261 ggccagccga accgccgcag ccgggcattc agcgggctca gcagcgccga gagcaacatg
    13321 gcgacggcta gcggcacagc cacgccggag aagtactgca gcagccacca gatcagcgcc
    13381 agcaccgcgg cgatcaccag cagccgccaa gaccactcgg ccgcggtgac gactccctgg
    13441 gggatgcgtg gtcccggcat gtcggcggga cggactccaa gccgagaacg tacggcccgt
    13501 cgggttcgtg gcgtaggcac gtggtcagcg tagccattgc gggtcggttg gaaacctgac
    13561 cggctcggtt ctcgacgccc ccgcattccc gcaagctgga cagttccgcc ccgtcttggg
    13621 acacaaccgt ccagnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngccccg
    13741 tcttgggaca caaccgtcca gcttgcgagg gaggtcgggc tagggtgacg cgcgtgagaa
    13801 cgcctctgcc acagctgatg gccggcgact gggctgaggc cctcgcgccg gtcgaatccc
    13861 agatcgtggc gatgggtgat ttcctgcggg ccgagatcgc tgccggacgc ggctatctgc
    13921 cgccaggccc gaacgtgctg tgggcgttcc agcggccgct ggcggacgtc cgggtgctga
    13981 tcgtcggcca ggacccctac cccacccctg gacacgccgt ggggctgtcg ttctcggtgg
    14041 cgcccgacgt gcgtccgctg ccacggagcc tggcgaacat ctacaccgag ctgaacaccg
    14101 acctgggtat ccggccgccg gagtccggcg acctgacccc gtgggccgac cagggcgtgc
    14161 tactgctcaa ccgggtgctg accgtgcggc cgggagcggc cgcgtcccat cgaggcaagg
    14221 gctgggaggc cgtcactgaa cgcgccatcg acgccctggt cgcacggggt ggcccgctgg
    14281 tcgcgatcct ctggggacgc gacgcccagt cactcgcgcc gcgcctggcc ggcgttccgc
    14341 agatcgcgtc cgcgcacccg tccccgctgt cggcgcacgc cggtttcttc ggctcgcgtc
    14401 cgttcagccg ggcgaacgca gccctcgtcg ctcagggcgg cgagccaatc gactggcgac
    14461 tcgcctgagc ggaacaagcg cggcgcgggc agggttctag aaggcatgtt cacgaagtcg
    14521 acgatggtcg cccccgaggc tgcattgccc ggacgcgagc gcggcctgct caccttgccg
    14581 gccttccacg aggtgctggg caccgacatc gccgccgcgc cggacggcgc cgaagtcgcc
    14641 tacttcgcgc tcggctgctt ctggggagcg gagaaactgt tctggaacac cgacggcgtc
    14701 ctgaccaccg cggtcggcta ccaaggtggc tacacaccga acgccaccta cgaagaggtg
    14761 tgctccggac gtaccgggca cgctgaggcg gtcaaggtgg tcttcgaccc caagcggacg
    14821 agctacgcca agctgctcaa ggtgttcctg gagaaccatg acccgaccca gggcatgcgc
    14881 caggggaacg acctcggcac ccagtaccgg tcggcgatct acttcaccga cgccgagcag
    14941 gaggccgcgg cgcgggccgc gatcgcggac taccagccga agctgagcgc gtccggctac
    15001 ggggcgatca ccaccgaggt ggtgccggcc accccgttct acttcgccga gcgctaccac
    15061 cagcagtacc tcgagaagaa ccccaacggc tactgcccca tccacgcgac cggggtgacc
    15121 tgcggctgag cgaacgcccc cgtcggggcg tccgggcggt cggtccgcgc cagagtggac
    15181 ggttccgcgc tgaatggacg cgaaagcgtc caccttgtgg ttgggcaagc ggcagcccgg
    15241 cgagcagatc cgttcgtcag gcatccgctg gtggcagatc ttggcactgg cggccgcccg
    15301 gtggccgagc tagcgtccag gccatgtgcc ggaacatcca cacccttcac aacttcgagc
    15361 cagcagccac cgacgccgag gtgcacgctg ccgcactgca gtacgtccgc aagatcgccg
    15421 gcacgacgaa accgtcaaag gcgaaccagg cggcgttcga cgaagcagtg gccgcgatcg
    15481 cgcacgcgac cgagcatctc ttggagcacc tgaccacgtc cgcgccgccg aagaaccgcg
    15541 acgttgaggc cgccaaggcc cgtgctcgct cggcatcccg ctacgcagtg ccggcctgag
    15601 ccctgcgagt accactggtc cgagaaccac gaccgggcgt catcgtgatc acgatgacgc
    15661 ccgatcgttg gtcgtgcgcg actactcagc ggctgccgcg agcttgtcca gtgccgactc
    15721 ccagcgctgc gtgatcgcgg cggcgtcgaa gtagaccggg atgtgctcat ggcggatctc
    15781 gacggtggtc tccgcttcac cggcggcggc cagatcgacc tcgacgatcg tcttcggcgc
    15841 ttcttcggcc gcgtgccagc tgaagcggat ctgctccagc gggtggtagg agcggatcac
    15901 gccgtacgtg ccgtcggccg cgtgccagct gtcgcccttg tcgccgagct cgccgcccgg
    15961 gcccagcagg gcctctgcac cctcgcgggt gaccaggagt ttccagaatt gcttgatggg
    16021 ttgggacacc gaccggctga cagtgacggt ggtgtccacg gggttcacga tcgtgtcgcc
    16081 ctggctctgt tcgttcatga gctacctcgt tccactcagt ggttccaaag gttccggagg
    16141 gtccggattg aagttcaccc tactcaggat ccgggccggc ggaagggtgt tcaccggagc
    16201 tcttgacgcc gtttttcgcc acacccgcgt gtcgctgcgc cgccagcgga cgcgcgccca
    16261 gtagacatat ggccaaggcc ggaccaggta tgagttggtc cggctttcga actccgcggg
    16321 tcgcccgaac cggtgctgct gtaccagctg agacagctga gacaagcagc ggaaaagtcg
    16381 tggcagatct ttcggaaagg ctcttgcgcg cccgcatccg gcgggcgtag aaaagttcta
    16441 cgcagatcac ggagccagaa ccgaaagggg agggacaccg gatcagcgga tagttgcaat
    16501 cgcggttcgc agcctgagga gcaatccaca ggaactccct ggacgggggt tgggccagtc
    16561 gggcaactgg gttcgggagg cgtccaggcc tgaacaactt gaaggccgga ccaactcata
    16621 cttggtccgg ccttcgcctt tgtctgcgca ccgaacctga acgaaatggg gacggggatc
    16681 accgcgcggc cgaactcgcc gtcccgctag ggtggtggcc cagacacacc aaccgtgcag
    16741 gaggtgagcc gatgctgatg accctgatca gcgctcacct cgtgatcgcc gtggtcgccc
    16801 cgatcctcac gcgagtgttc gggcaacgcg tcttctgggt cgcagcgctg gctcccgcca
    16861 tcagtttcgc ctggctgctg agccttgccc ccgacgtgct ggcgggcgtg ccgttggtcg
    16921 agaagaccga ctggatcccc gccctcggtg tctcggtgaa cttccacctc ggcctgctgc
    16981 agtgggtcct ttccctgatc gtcaccggca tcggtgccct cgtcctggcc tactgccgct
    17041 ggtacttcat cgcggacgcg ccgcagcggc gggtggtcgg cctgctgacc gccttctgcg
    17101 cggccatgat cggtctggtc accgcggacg acctgatcgc gatctacgtc ttctgggaag
    17161 ccaccacggt cttctcctac ctcctgatcg gccacgaccc gacccgccgc gccaac
//
LOCUS       DAOPRQ010000055        17035 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_2829_length_17035_cov_12.998645, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000055 DAOPRQ010000000
VERSION     DAOPRQ010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 17035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17035
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2829_length_17035_cov_12.998645"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1550)
                     /locus_tag="PKJ61_08245"
     CDS             complement(<1..1550)
                     /locus_tag="PKJ61_08245"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HOC13855.1"
                     /translation="MFTLDPYAARSCALKTHHAFHPGVVKPSTPVPPSRLPGSAEFVA
                     ATYARILAGRAVVVDLRSLHGEPSEAQEAACLAAMADQTEVIIGGLLPRDWEGHRSSR
                     ADLLVRSPDGGYFPGLVKYQRVVDLRKDDFEFVYSELAELPARCSTTGRRYRWHWRWA
                     NALQLAHLWRHLSVLGHAAKSAPAGLVIGNDEAFDQVACWLELTVASCPPAPTQLDAS
                     AEGTVQVSALDRYDHDFAARVELAELASAANPDDPPLLKPIVCYECSYCPWWAYCRSQ
                     LDDDDLSLRINKSPLDGHEISVLRESGVATVTELAGVDLDSLLPDYLPRVVHRTGAED
                     RLRLAQRRSRLLLSGVELERTATGPIDIPKACIEIDIDVETSRDDRVYLWGFWVSEPD
                     DGGYYQEFSSFTDLDEAAELELARQAMAWLRDVTEGSESLVFHYSDYEVVRLTRLAKS
                     GAAEVVWAVEYAREHFVDLFSIVRKQFFGTNGLGLKVVASAGAGFHWRDDDPGGLNSM
                     RWFDEAVHA"
     gene            complement(1550..2332)
                     /locus_tag="PKJ61_08250"
     CDS             complement(1550..2332)
                     /locus_tag="PKJ61_08250"
                     /EC_number="2.1.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002545727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="demethylmenaquinone methyltransferase"
                     /protein_id="HOC13856.1"
                     /translation="MLSLGRPTATFGEGSVVSARHGRLFLVRATNEARATLARRRAEV
                     SAMFDRVAPRYDVVNDILSLGQDRRWRAETVAAVAPRPGQVILDLAAGTGTSSRPFAD
                     AGAVVVAADLSEGMLVVGKERQPDLAFVNADALALPFADAAFDAVTISFGLRNVEDVA
                     NALAELRRVTRPGGRIVICEFSTPTWKPFRTTYRNYLLQALPKIAGMTASNPVAYEYL
                     AESILAWPDQRGLADLMFDSGWRGVGWKNLSGGIVALHRAHA"
     gene            2374..3033
                     /locus_tag="PKJ61_08255"
     CDS             2374..3033
                     /locus_tag="PKJ61_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016456817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOC13857.1"
                     /translation="MARKTPVLKVTNLKKAFGPVVIVDGFNLEVAAGEAVALTGRNGA
                     GKSTVLRCLIGADRADDGTVEVNGVPVRETDPQIRRDVATVIDDLDFFPDLSVVEHLD
                     LLARAHGIPDADGAVDEVLHEVQLVPQSGQLPGTLSSGQRRRLALATAFIRPRKLLIL
                     DEPEQRLDTEGVAWLAARLVAERQRGLAIVMASHEPSLVEQVATRVVRLGDRAEVLAD
                     E"
     gene            3026..4744
                     /locus_tag="PKJ61_08260"
     CDS             3026..4744
                     /locus_tag="PKJ61_08260"
                     /inference="COORDINATES: protein motif:HMM:NF039718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6297 family protein"
                     /protein_id="HOC13858.1"
                     /translation="MSKKSRKLIRSTPTEPVVEVEASAPEPVEERSFYFLADVEPVRV
                     DERELHEEMKLWRHGRATRTIGQALQDGYVMIFTIVVLGAMITSAVLKAQTGMAGCDT
                     EACASGRLLLPSAMVLACFGLALGVSRMFGPVLASAAEGFWLMDAPISRRRLLRARMR
                     LPIIVALLVAGLATALVSALSGLSWWQVLAWSLAAGIGSAALVAFAASEQPLERTRVV
                     KLLQGVFAAGALALLLVVVAVGAGWLPVAVVADAGQGLWVMVGLAVLAAVALVVLLGL
                     ATSRLEQIRRARLLSGGSLVSGMQGAMFALDFGLIRDILVERRMAEKGHVKPTRGRGL
                     GLSALIWRDVERLKRSPGAFVGLAVSLFVPYAADALRMSQLNPMISGLALVAGLVPFL
                     GSLRVITRTGGLARSFPFKTTQLRMAAMVVPAALALVWGVLATPAFIGIAQTGVDRSI
                     GDGFIAALVTAVAGLLGAVRWVTAKKVDFGTPMMATESGAMPPTLIFNLFRGFDMVAL
                     ITAPLILQAPAYWSLAIAAVVFVALRGSFNMEEMKAEQEAQQKALAAAKAEKQLEKQK
                     IPRPSR"
     gene            4826..5536
                     /locus_tag="PKJ61_08265"
     CDS             4826..5536
                     /locus_tag="PKJ61_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020579591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOC13859.1"
                     /translation="MADAIASGPQPKHRQLRELLIGMVVPGKAIPSERELMATYGVSR
                     ATVRKAIDGLVADGLLQRTHGLGTFAVRPRLETRLHLASFSQDMRRRGRVPSTRLVSM
                     TRAAAPVEVARALHLETGADAWRVIRIRFADGEPLAHEDGWYPAGLFLDLDRQDVGDG
                     SLYEIFGQQYGLWIDHAEQTLWGENASAKVARLLAARTDTALLVFRRTSTAAGRPIEY
                     VVSRYRGDRYEVHMELSN"
     gene            5589..6380
                     /gene="nagB"
                     /locus_tag="PKJ61_08270"
     CDS             5589..6380
                     /gene="nagB"
                     /locus_tag="PKJ61_08270"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008354591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HOC13860.1"
                     /translation="MEVIICPNAQRVGEVAAAKAARIAAPIGPKVVLGVATGSSPVQT
                     FAELAKLARSGELDLSEASAFALDEYVGIAAGHPESYHEIIRKTVTEPMGLDPARVHV
                     PNGFAEDLAAACREYEDAIRAVGGVDIQFLGVGTNGHIGFNEPTSSLSSRTRIKTLAR
                     LTREDNARFFNSIDEVPKHCLTQGLGTIMDARNVILVATGPGKAHAIAQVIEGPITSM
                     WPGSVLQLHQHATIVVDEAAAGDLALADYYRETYANLPDWQRLDV"
     gene            6380..7504
                     /gene="nagA"
                     /locus_tag="PKJ61_08275"
     CDS             6380..7504
                     /gene="nagA"
                     /locus_tag="PKJ61_08275"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020574302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HOC13861.1"
                     /translation="MLLHADRILFPDSTGPASGWIEIAGDRIVGTGLGPAPGVVDEEL
                     DGVVVPGYVDVHSHGGGGASFVTEDPDVARQALAAHRRLGVTTMVASLVTGTIEDLER
                     QVSCLAGLVESGELAGIHLEGPWLALKYKGAHPPLLLADPDPAVVARLLAVGRGAVRM
                     VTIAPERKGALESIRLMAANGVVAAIGHTEADYDTCQRAIESGATGATHLFNAMAPLR
                     HREPGPVLALWEDRRVYLELIMDGVHVRPELVAFVMATEPDRVVLVTDAMAAAAAADG
                     DYVLGELPVEVREGVARIAGTDTIAGSTLTLDRAVRNAVAAGVPLAQAVRSATSLPAD
                     YLGLTEVGRIVAGKRADLVVLDESLAAARVLHRGAWLDGH"
     gene            7579..8541
                     /locus_tag="PKJ61_08280"
     CDS             7579..8541
                     /locus_tag="PKJ61_08280"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOC13862.1"
                     /translation="MSYEPPVKTRVRWGRVALFYAIALGWVSLIAALLYLANQRNLSG
                     AAGPTVLTVVLALLYMPAPLVAALIVGRLDHRPALIRTTFQGFWRKLPKLIGVALTVI
                     PALLLGMVGVSWVVGNLIGFDAAGTMLFTHEDLVANTLTLVPGMNADQVAAMGASVPP
                     FWGLIGMAFLGAIVAGFTINGLFAFGEEYGWRGWLADELRPLGAFWVNVITGVLWGLW
                     HAPLILMGFNYGEYTRLGPFFMVAWCVAASFLLWRAREVTGSLLAPAVLHGAINGFAG
                     VFTVLLAGANPLVAVPMGVLGSGTVAVVAAVFWWLTRKRTQPAA"
     gene            8505..11732
                     /locus_tag="PKJ61_08285"
     CDS             8505..11732
                     /locus_tag="PKJ61_08285"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOC13863.1"
                     /translation="MADQKADATRRMTTPKYPEWVHELSERRLVEMFGELTLARGQEY
                     VRQSRVGHIAVGSGTGGSLVQAQVSGSSYRAYQTVVRHHADSGVITTSCSCPVGHDCK
                     HAAALLWHMRTVNLRAVTPAWQRALTDLVAEGRSTAQGLPLAIQVTHTNGGTQLRPMM
                     WGRSGRWVRSGITWDSVESPWSGDYLAAHRAALAGIASTRDQRGSYRYLYSRRADVLE
                     LGEVSAGVWSRLRQAVAAGVELIPASGTPSVRLAEPARLTSRLSQDADGLALDTVVVA
                     DDREWPLRYGDLMGEPPHGFAVIAASELLLVPFAEPLSQGQTALLLQHPHLRIPASDV
                     PAFAADFYPALCRMVAVEVEPGVELPSVEAPRLRLGVAFEPEHVTKLRWSVRYRVGDT
                     ANDLDLYPVGVQPGYRDLAAEAALLDALPAGPWPRARGAGDRWQVAETATLRGPATAE
                     FATTGLATLRAAGVIVEVSGDPVAYRLATAAPEVSLEVSDPAEGTDWFNLSVSVTIEG
                     EAVEFRELFAALATGQTHLILPSGVWFSLDRPELAQLRALIEEARELQPREGEGLRLR
                     AEHAGLWEELVSLGVVAEQSAAWTRAVSALLDVESLPEVPVPDGLHATLRPYQELGFR
                     WLRFLGNARLGGILADDMGLGKTMQALALASSLKEGGELDRPMLVVAPTSVLGTWEAE
                     AAKFAPGLDVRVLAQTSKKRTAPLADIVAGADLVVTSYTLLRLDEEEYVGLPWSVALL
                     DEAQFVKNRQSRGYQSVRKLRARTKFAITGTPLENNLMDLWSLLSIVAPGLFADPVAF
                     AERYRRPIENGSDPAALGRLHRRVRPLMLRRTKETVASELPPKQEQVLSVELSPAHRR
                     LYDKQLAAERKQVLGLVDDLKRNRITILASLTRLRQLALSPALVLPGHGAASAKIDTL
                     IELVTELAAEGHRALVFSQFTTYLGMVRERLAEEGIGTSYLDGRTRDRPARIAEFREG
                     TNPVFLISLKAGGFGLTLTEADYVFILDPWWNPAAELQAIDRTHRIGQDQPVMVYRLV
                     SADTIEEKVVALQERKRDLFAKVVGEAGDLAAPLSAEDIRGLLEP"
     gene            11781..12035
                     /locus_tag="PKJ61_08290"
     CDS             11781..12035
                     /locus_tag="PKJ61_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13864.1"
                     /translation="MTSRAAYLAAFEAYRRALEAEGFAVSDVRTDERGLLTWGAGDAE
                     SPAEVARLDEIHEQHFKTAEREYVREVFDRIYGTDRHGSV"
     gene            complement(12294..13598)
                     /locus_tag="PKJ61_08295"
     CDS             complement(12294..13598)
                     /locus_tag="PKJ61_08295"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014847377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase"
                     /protein_id="HOC13865.1"
                     /translation="MIQHPPRTRLRASSRAIDEVGNLLAYLRTEGVAWLRRGDGMVGV
                     GEVARFSGTFTEAEAWWQEFCAQLDGGALQLECGPVGPVAFASFVFDPATTAQQSVLV
                     VPRVLIGRRKGCSWLTEISDGLAPDATAEPVEPDNPRPVEHAETAAAHTASQSVRKVA
                     ESLDREQWQDIVEQAVGRIGQGEIDKVVLARGVTLEADEPLDGRTLVRELADRYPSCW
                     TYYVDGLVGASPEMLIRREDGLATSRVLAGTIRRNGAEELDLKLAHALARSGKNLIEH
                     ELAVESVARALAPFCSGMNVPDAPYVLELPNVLHLASDVTAVAHPDVSALRLAGELHP
                     SAAVCGTPTEVARKVIAELEHLDRGRYSGPVGWLDANGDGEFAIALRCGLLDPSDPRR
                     ITIYAGCGIVSESDPAEEYAETEAKLLPMLEALGVPTPVHHD"
     gene            13712..15010
                     /locus_tag="PKJ61_08300"
     CDS             13712..15010
                     /locus_tag="PKJ61_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014167317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl reductase family protein"
                     /protein_id="HOC13866.1"
                     /translation="MSEPEPAIPATGSPVEADVIVVGAGPGGSAAAAHLAGRGLDVVL
                     LEKSKFPREKVCGDGLTPRAVKQLIKLGIDTSEEAGWVHNRGLRVYGGRTEPFELPWP
                     DLAEFPPYGLVRARATFDKILADRAVAGGARLYEQTNVTAPIINERTDRIVGVQSKDG
                     RTFRAPVVIAADGNSARLALGMGLAKRTDRPMGVAVRAYFNSPARTNDDHMESWLELW
                     DGKPGESSLLPGYGWIFGMGDGTVNVGLGTVSSSSEPAKMNYREMFSRWLANTPAEWG
                     FTPENQVGPTLGAALPMSFNRQPAYARGLLLVGDSGGMISPFNGEGISYAMEAAEMAA
                     DAIADARFRGFGTPSAETALRGYPARLKAEWGGYFRLGQVFVSLIEHPQVMHLCTRYG
                     LPRPTLMRFTMKLLAHLYDTKDGDWMDKVIRGLAKVAPSA"
     gene            15054..15413
                     /locus_tag="PKJ61_08305"
     CDS             15054..15413
                     /locus_tag="PKJ61_08305"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017948722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HOC13867.1"
                     /translation="MSPYIPLLGIMALAAGFLAISLLLSAFVGPRRYNKVKYSSYECG
                     IEPTPQPAGGGRFPIKYYIIAMLFIVFDIETIFLYPWAVTFKQLGLFALLEMVLFMAT
                     VFVAYAYVWRRGGLEWD"
     gene            15432..15989
                     /locus_tag="PKJ61_08310"
     CDS             15432..15989
                     /locus_tag="PKJ61_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020391928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HOC13868.1"
                     /translation="MGIEDKIPQGVVLTTVEGIFGWMRQASFWPATFGLACCAIEMMA
                     YGTPRFDAGRWGQEVFRASPRQADLMIISGRVSQKMAPVLRQVYDQMPNPKWVLAMGV
                     CASSGGMFNNYAIVQGGDHLVPVDMYVPGCPPRPDMLIDAMWKLRKDIVAKRPIGQNE
                     LDAYEAAETAALTAPATHEMKGLMR"
     gene            15986..16756
                     /locus_tag="PKJ61_08315"
     CDS             15986..16756
                     /locus_tag="PKJ61_08315"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HOC13869.1"
                     /translation="MSDPKLPGEEKSIDKPVMDPEASPKAGVEREDAQPDPELTPAII
                     EAPKPIAVKKGMWGSGTGDTSGYGRIVKPVEWPAPSAPPYGSWFDGVHDRLAALVADP
                     FIKVVLDRGELTFHIPRAKLVEVMQALRDDAQLRFEFCASVSGVHYPQAAGEELHVVY
                     HLQSMTHNRRLRVETSCPEADAHVPSIVAVYPAADWHEREAWDMFGIIFDGHPHLTRI
                     LMPDDWVGHPQRKDYPLGGIPIEYKGTTVPPVDQRRSY"
     gene            16825..>17035
                     /locus_tag="PKJ61_08320"
     CDS             16825..>17035
                     /locus_tag="PKJ61_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020578391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase subunit D"
                     /protein_id="HOC13870.1"
                     /translation="MGGGDWGDIASEQAERGDETLVINMGPQHPSTHGVLRLMVECDG
                     ETVQSIRPGIGFLHTGIEKNMEYRSW"
BASE COUNT         2659 a         5677 c         5926 g         2773 t
ORIGIN      
        1 gcgtgcacgg cctcgtcgaa ccagcgcatg ctgttcaggc cgcccgggtc gtcgtcgcgc
       61 cagtggaagc ccgccccggc ggacgcgacc accttcagcc cgagaccgtt ggtgccgaag
      121 aactgcttgc gcacgatcga gaacaggtcg acgaagtgct cgcgggcgta ttccacagcc
      181 cacaccacct ccgcggcacc gctcttggcc agacgggtca ggcgcacgac ctcgtagtcg
      241 gagtagtgga aaaccagcga ctcgctgccc tcggtcacat cccgaagcca ggccatcgcc
      301 tgccgggcca actcgagctc ggccgcttcg tcgaggtcgg tgaagctgct gaactcctgg
      361 tagtacccgc cgtcgtcggg ctcgctgacc cagaagcccc aaagatagac ccggtcgtcg
      421 cgagaggttt cgacgtcgat gtcgatctcg atgcaggctt tcgggatgtc gatcggcccg
      481 gtggccgtcc gctccaattc gactccgctg agcagcagcc gtgagcgtcg ctgcgccagt
      541 cgcagccggt cctctgcgcc ggtgcggtgc accacccgcg gcaggtagtc gggcagtagc
      601 gagtcgaggt cgacaccggc cagctcggtc accgtcgcca ccccggactc gcgcagcacg
      661 gagatctcgt gcccgtccag cggcgacttg ttgatccgca gcgagaggtc gtcgtcgtcc
      721 agttgcgatc ggcagtacgc ccaccacggg cagtaggagc actcgtagca gacgatcggc
      781 ttgagcagcg gcgggtcgtc ggggttggcg gcggacgcga gttcggccag ctcgacccgg
      841 gcggcgaagt catggtcgta gcgatccagc gcgctcacct gcaccgtgcc ttcggcggac
      901 gcatcgagct gggtcggcgc gggtgggcag gatgcaacgg tcagctccag ccagcaagcg
      961 acctggtcga acgcttcgtc gttgccgatc accagaccgg ccggcgcgct cttcgcggca
     1021 tgaccgagca cgctcaggtg ccgccacagg tgcgccagct gaagcgcgtt cgcccaccgc
     1081 cagtgccagc ggtacctgcg tccggtggtc gagcagcgag ccggcagttc ggccagttcc
     1141 gaatacacga actcgaagtc gtccttgcgc aggtcgacca cgcgctgata cttcaccagt
     1201 ccggggaagt agccgccgtc cggcgagcgc accagcagat cggcgcggct ggagcggtgc
     1261 ccttcccagt cccggggcag caggccgccg atgatcactt cggtctggtc agccatggca
     1321 gccaggcagg cggcttcctg cgcctcgctc ggctcgccgt gcaggctccg caggtcgacg
     1381 accaccgcgc gtccggcgag aatgcgagca taggtggcag cgacgaattc agcgctgccc
     1441 ggcaaccgac tcggtgggac aggcgtgctc ggcttgacca cgcccggatg gaaggcgtgg
     1501 tgggtcttca gtgcgcagct gcgggccgcg tagggatcga gggtgaacat caggcgtgcg
     1561 cgcggtgcag ggcgacgatg cctccgctca ggttcttcca gcccacgccg cgccagccgg
     1621 agtcgaacat caggtcggcc agcccgcgct gatcgggcca ggcgaggatc gattcggcaa
     1681 gatactcgta ggccaccggg ttggacgcgg tcatcccggc gatcttgggc agcgcctgca
     1741 gcaggtagtt gcggtacgtc gttcggaacg gcttccaggt aggtgtggag aattcgcaaa
     1801 tgacaattcg gccaccgggg cgggtcaccc ggcggagttc cgccaacgcg ttggccacgt
     1861 cctcgacgtt gcgcaggccg aagctgatcg tcaccgcgtc gaaggcggcg tccgcgaacg
     1921 gcagcgcgag cgcgtccgcg ttcacgaagg cgagatccgg ctggcgctcc ttgccgacca
     1981 ccagcatgcc ttcggagaga tcagcggcca cgaccaccgc acccgcgtcc gcgaatggac
     2041 ggctggacgt accggtgccg gccgccaggt cgaggatcac ctgaccggga cgcggggcga
     2101 ccgcagccac ggtttcagct cgccagcgcc gatcctgacc gagggagagg atgtcgttca
     2161 cgacgtcata gcgcggcgcc acccgatcga acattgccga cacctcggcc cgtcttctcg
     2221 ccagagtcgc tcgggcctcg ttcgtcgcac gcacgaggaa tagcctgcca tgccgcgccg
     2281 acacaaccga cccctcgccg aaagtggcag tcggacgccc gagggaaagc atcggtggtc
     2341 ggggcacgac ctgggatagg ggagaatcga cgtatggcgc gcaagactcc ggttctgaag
     2401 gtcaccaatt tgaagaaggc cttcgggcca gtggtgatcg ttgatgggtt caacctcgag
     2461 gttgccgcgg gcgaggccgt ggccctcact ggacgcaatg gcgccggcaa gtccacggtg
     2521 ctgcggtgcc tgatcggggc cgatcgtgcc gacgacggga cggtggaggt caacggggta
     2581 cccgtccgcg agaccgaccc gcagatccgt cgcgacgtgg ccacggtgat tgacgacctg
     2641 gatttcttcc cagacctgag tgtggtggaa cacctcgacc tgctcgcgcg cgcccatggc
     2701 atcccggacg cggacggtgc cgttgacgag gtcctgcacg aggtccagtt ggtgccgcag
     2761 tcgggacaac tgcccggcac gctctcgtcc ggccagcgcc ggcggctcgc gctggcgacg
     2821 gcgttcatcc gtccgcgcaa gctgctgatc ctggacgaac cggagcagcg actcgatacc
     2881 gagggcgtgg cctggctcgc cgcccggctg gtcgccgagc gccagcgtgg cctcgcgatc
     2941 gtgatggcca gccacgagcc gagcctggtg gaacaggttg ccacgcgcgt cgtccggctt
     3001 ggcgaccgcg ccgaggtgct cgccgatgag taagaagtcc cgcaagctga tccgttcgac
     3061 accgaccgaa ccggtcgtcg aggtcgaggc gagtgcgccg gagccggtcg aagaacgctc
     3121 gttctacttc ctcgccgatg tcgagcccgt ccgggtcgat gagcgcgagc tgcacgagga
     3181 gatgaagctg tggcgccacg gccgcgccac ccgcacgatc ggccaggcgc ttcaggacgg
     3241 ctacgtgatg atcttcacga tcgtcgtgct gggcgccatg atcacgtccg ctgtgttgaa
     3301 ggcccagacc ggcatggctg gatgcgacac cgaagcctgt gcgtccggac gcctgctgct
     3361 gccgtccgcg atggtgctgg cctgtttcgg cctcgcacta ggggtttcgc ggatgttcgg
     3421 gccggtgttg gcctccgctg ccgaaggttt ctggttgatg gacgccccga tcagccgacg
     3481 ccggctgctg cgcgcccgga tgcggctgcc gatcatcgtg gcactgttgg tggccggcct
     3541 ggccaccgcg ctggtgtcgg cgctgtccgg gctgagctgg tggcaggtgc tcgcttggag
     3601 cctcgccgct ggcattggca gtgccgcatt ggtggccttc gccgcgtccg agcagccgct
     3661 cgagcgcacc cgggtcgtga agttgctgca gggcgtgttc gccgccggcg cactggccct
     3721 gctgctggtg gtagtggctg tcggtgccgg ctggctgccg gtcgctgtgg ttgccgacgc
     3781 cggccagggg ctctgggtca tggttggcct agcggtgctg gccgcggtag cgctggtggt
     3841 gctgctcggc ctggccacgt cccggctgga gcagatccgg cgggcgcggc tgttgtcggg
     3901 tggttcgctg gtcagtggca tgcagggtgc gatgttcgct ctcgacttcg gactgatccg
     3961 cgacatcctg gtggagcggc gcatggccga gaagggccat gtcaagccga cccggggacg
     4021 cgggttgggc ctttcggccc tgatctggcg cgacgtcgag cggctgaagc gctcgcccgg
     4081 ggcgttcgtc ggcctggcgg tgagcctgtt cgtgccgtac gcggcggacg cgctgcgcat
     4141 gagccagctg aacccgatga tcagcggtct tgcgctcgtg gccggcctgg tgcccttcct
     4201 gggcagcctg cgggtgatca cacggacggg tggactggcc cggtccttcc cgttcaagac
     4261 cactcagttg cggatggccg caatggtggt gccggcggca ctcgccctcg tctggggagt
     4321 cctggcgact cctgcgttca tcgggatcgc ccagaccggg gtcgaccgca gcatcggtga
     4381 cggcttcatt gctgctctgg ttaccgcggt cgccggtctg ctcggcgcgg tccgctgggt
     4441 gacggcgaag aaggtcgact tcggcacgcc gatgatggct accgagtccg gcgccatgcc
     4501 gccgaccctg atcttcaacc tgttccgcgg cttcgacatg gtcgcactga tcacggcccc
     4561 gctgatcctg caggcacccg cctactggtc ccttgcgatc gccgcggtgg tcttcgtggc
     4621 gctgcgtggc agtttcaaca tggaggagat gaaggccgag caggaagccc agcagaaggc
     4681 gctcgccgca gccaaggccg agaagcagct ggagaagcag aaaattccgc gtccgagtcg
     4741 ctgaggacga tcggcgttgg cccgattact tgccacgcag cctttggcct agagtggtac
     4801 gtaccagtac ggaccaggag gcgccatggc ggacgcaatc gcgtcagggc cgcaacccaa
     4861 gcaccggcaa ctgcgtgaac tgctgatcgg catggtcgtg ccgggcaagg cgatcccgtc
     4921 cgagcgcgaa ctgatggcaa cctacggcgt ttcccgcgcc accgtccgca aggccatcga
     4981 cgggttggtc gcggacggac tgctgcagcg cacccacggt ctgggcacgt tcgccgtccg
     5041 gccccggctg gagacccgac tgcatctggc ttcgttcagc caggacatgc gcaggcgtgg
     5101 acgcgtcccg tccacgcgcc tggtttcgat gactagagcg gcagcgccgg ttgaggttgc
     5161 tcgcgcgctc cacctcgaaa ctggagctga tgcttggcgg gtgattcgaa ttcggttcgc
     5221 cgacggtgag ccgctcgccc acgaggacgg ctggtatccg gccggactgt ttctcgacct
     5281 cgatcgccaa gacgtcggcg acgggtcgtt gtacgagatc ttcggtcagc agtacgggct
     5341 gtggatcgat cacgcagagc agacgctctg gggcgagaac gcgtccgcca aagtcgcgcg
     5401 gttgctcgca gcccggacgg acacggcgct gctcgtcttc cggcggacgt ccaccgccgc
     5461 cggcagacca atcgagtacg tggtctcgcg ctaccgcggg gaccgctacg aggtgcacat
     5521 ggaactgagc aattagcagg ctctcccagg ccgagcgcaa gtcacgaact gaacacttcg
     5581 gagttgaaat ggaagtaatc atctgtccca acgcccagcg ggtcggcgag gtcgccgcag
     5641 ccaaggcggc gcgaatcgcc gcgccgatcg gtccgaaggt ggtgctcggc gtggccaccg
     5701 gctcgtctcc ggtgcaaacc ttcgccgagc tggccaagtt ggctcgttcc ggcgaactgg
     5761 acctctcgga agcgtccgct ttcgccctgg acgagtacgt gggcatcgcc gcgggccacc
     5821 cggagagcta ccacgagatc attcgcaaga cggtgaccga gccgatgggg ctcgacccgg
     5881 ctcgcgtcca tgtcccgaac ggcttcgccg aggaccttgc cgcagcctgc cgggagtacg
     5941 aggacgcgat ccgcgcagtc ggcggcgtgg acatccagtt cctcggggtg ggtaccaacg
     6001 gccacatcgg gttcaacgag ccgacgtcct cgctgtcgtc ccgcacccgc atcaagacgc
     6061 tcgcccggct cacccgtgag gacaacgcgc ggtttttcaa ctccatcgac gaggtgccga
     6121 agcactgcct cacccaaggg ctcggcacga tcatggatgc gcggaacgtg atcctggtcg
     6181 cgactggtcc gggcaaggcc cacgcgatcg cccaggtgat cgagggcccg atcacctcga
     6241 tgtggccggg ctcggtgctg cagttgcacc agcacgccac gatcgtcgtg gacgaagccg
     6301 ccgcgggcga cttggcgctc gccgactact accgcgagac ctacgccaac cttcccgact
     6361 ggcagcgact ggacgtctga tgcttctcca cgcagaccgg atcctgttcc cggactcgac
     6421 cgggcccgcg tccgggtgga tcgagatcgc cggcgaccgg atcgttggca ccggcctcgg
     6481 tccggcacct ggcgtggtgg acgaggagct ggacggcgtc gtcgtccccg gctacgtgga
     6541 cgtgcacagc cacggcggtg gtggggcatc gttcgtgacc gaggaccccg acgtggcgcg
     6601 gcaggcgctg gccgcccacc ggcggctggg cgtgaccacg atggtggcct cgctggtcac
     6661 cggcaccatc gaagacctgg agcggcaggt ttcctgtctt gcggggttgg tggagtccgg
     6721 cgagttggcc ggcatccacc tcgaagggcc gtggttggcg ctcaagtaca agggtgcgca
     6781 tccgccgctg ctgctggccg atcccgaccc ggcggtggtg gcgcggctcc tcgctgtcgg
     6841 ccgcggcgcg gttcggatgg tcacgatcgc gcccgaacgg aagggggcgc tcgagtcgat
     6901 ccggctcatg gccgccaacg gcgtggtggc cgcgatcggg cacaccgaag cggactacga
     6961 cacctgtcag cgggcgatcg aatcgggcgc gaccggcgca acgcatctgt tcaatgcgat
     7021 ggctccgctg cggcatcgcg agcccggtcc ggtgctggcg ctgtgggagg accggcgggt
     7081 ctacctcgag ctgatcatgg acggagtgca cgtccggccc gaactggtgg ccttcgtgat
     7141 ggccacggag cccgaccggg tggtgctggt aaccgacgcc atggccgcgg cggctgctgc
     7201 ggacggcgac tatgtgctgg gcgagctgcc ggtggaagtt cgcgagggtg ttgcccggat
     7261 cgccggcacc gacaccatcg ccggctcgac gctcactctt gaccgcgcgg tccgcaacgc
     7321 cgtcgcggcc ggggtgccgc tggcccaggc cgtccgctcg gccacctcgc tgccggcgga
     7381 ctatctcggt ctcaccgagg tcggccggat cgttgccggc aagcgcgccg atctggtggt
     7441 gctggacgag tccctggccg ccgcccgggt gctgcaccgt ggcgcctggc ttgatggcca
     7501 ctgagcctga cgaagggccg tgagcctgtc gagaccgcga cgcgcgcact gtccgagcca
     7561 gcggctacgg tgtgaggcgt gagttacgag ccgccggtga agacacgcgt ccgttggggc
     7621 cgggtcgccc tgttctacgc gatcgcgctg ggttgggtga gcctgatcgc ggccctgctc
     7681 tacctggcga accagcgaaa cctgtccggc gccgccgggc cgaccgtgct taccgtggtg
     7741 ctggcgctgc tctacatgcc ggcaccgctg gtcgcggcgc tgatcgtcgg acggttggat
     7801 caccgaccgg ccctaatccg aaccaccttc cagggcttct ggcgcaagct accgaagctc
     7861 atcggagtcg cgctcacggt gatccccgcg ctgctgttgg gcatggtggg cgtgagctgg
     7921 gtggtcggca acctgatcgg cttcgacgct gccggaacca tgctgttcac ccacgaagac
     7981 ctcgtggcca acaccctcac gctcgtcccc ggtatgaacg ccgaccaggt ggccgccatg
     8041 ggcgcatccg tgccgccctt ctgggggctg atcggcatgg ccttcctcgg tgcgatcgtc
     8101 gccgggttca ccatcaacgg tctgtttgcc ttcggtgagg agtacggctg gcgcggttgg
     8161 ctggccgacg aactgcgtcc gctgggcgcc ttctgggtga acgtgatcac cggcgtgctg
     8221 tggggccttt ggcacgcccc actgatcctg atggggttca actacggcga gtacacccgg
     8281 ctcggaccgt tcttcatggt ggcctggtgc gtggcggcgt ccttcctgct ctggcgcgcc
     8341 cgtgaagtca caggctcgct gctggccccg gcagtgctgc acggcgccat caatggcttc
     8401 gccggcgtgt tcacggtgct gctggccgga gctaacccat tggtcgccgt accgatgggg
     8461 gtgctgggct caggcacggt agcggtcgtc gccgcggtgt tctggtggct gaccagaaag
     8521 cggacgcaac ccgccgcatg acgacaccca agtatcccga atgggtgcac gagttgagcg
     8581 agcgccggct cgtcgagatg ttcggcgaac tcaccctcgc ccgtggccag gagtatgtac
     8641 ggcagtcacg ggtgggacac atcgcggtcg ggtcgggcac cggcggcagt ctggtgcagg
     8701 cccaggtcag cggatcgagc tatcgcgcct accagacggt cgtacggcat cacgcagaca
     8761 gcggagtgat cacgacgtcc tgctcctgtc cggtcggcca cgactgcaag cacgccgccg
     8821 cactgctctg gcacatgcgg acggtgaacc tgcgcgcggt gacgccggcc tggcagcgtg
     8881 ccctcaccga cctggtcgct gagggccgct cgaccgccca gggactgccg ctggcgatcc
     8941 aggtgaccca taccaacggt ggcacccaac tgcgtccgat gatgtgggga cgatcgggac
     9001 gttgggtgcg cagcggcatc acctgggaca gcgtcgaatc gccatggagc ggggactacc
     9061 tggccgcgca ccgagccgca ctggccggaa tcgcgtccac ccgtgaccaa cgcggcagct
     9121 accgctacct gtattcccga cgcgcggacg tgctcgaact cggcgaggtc agcgccgggg
     9181 tgtggtcgcg gctgcgtcag gcagtggcgg cgggggtcga gctaatcccg gcgtccggca
     9241 cgccgtcggt ccggttggcc gaaccggcgc ggctaaccag ccggctgagc caggacgccg
     9301 atggcctggc gctggacact gtggtggtcg cggacgaccg cgaatggccc ctgcgctacg
     9361 gcgacctgat gggggagccg ccgcacggct tcgcggtgat cgctgccagt gaattgctgc
     9421 tggtgccgtt cgccgagccg ctcagccagg gtcagaccgc cctgttgctg cagcacccgc
     9481 acctacggat cccggcgagc gatgtccctg cgttcgccgc cgacttctat ccggcgctgt
     9541 gccggatggt cgccgtcgag gtggagcccg gcgtcgagct gccgtcggtc gaggcgccgc
     9601 ggctccggtt gggcgtggct ttcgagcccg agcacgtgac caagctgcgc tggagcgtgc
     9661 gctatcgagt cggggacacc gccaacgacc tggatctcta tccggtcggc gtacagcccg
     9721 ggtatcgcga tctggccgcg gaagccgccc tgctggacgc tttgcctgcg ggcccctggc
     9781 cgcgggcccg tggcgccggc gaccgctggc aggtggcgga gaccgcgacc ctgcgtggac
     9841 cggccaccgc tgagttcgcc actaccggtc tggctacgct gcgcgctgcc ggggtgatcg
     9901 tggaggtcag cggtgatccg gtcgcctacc ggctggccac ggcggcgccc gaggtcagcc
     9961 tcgaggtgag tgatccggcc gaggggacgg actggttcaa cctttcggtc agcgtcacca
    10021 tcgagggcga ggcggtcgag ttccgcgagc tcttcgcagc gctggccacc ggacagaccc
    10081 acctgatcct gccgtcgggg gtgtggttca gcttggatcg gcccgaactg gcccaacttc
    10141 gcgcgctgat cgaggaggcc cgggaactgc agccgcgcga gggcgagggg ctgcggctgc
    10201 gcgccgagca cgccgggctg tgggaggagc tggtcagcct cggggtggtg gctgaacagt
    10261 cggcggcctg gacgcgggcg gtgtcagcgc tgctggatgt cgagtcgctg cccgaagtgc
    10321 cggtgccgga cgggttgcac gcgacgctgc gtccctacca ggaactcggc ttccgctggc
    10381 tgcgattcct cggcaacgcc cggctgggcg gcatcctggc cgacgacatg ggcctgggaa
    10441 agaccatgca ggcgctcgcg ctggccagct cgctgaagga gggcggcgag ctcgatcggc
    10501 cgatgctggt ggtggcgccg acgtccgtgc tgggcacgtg ggaggcggag gctgcgaagt
    10561 tcgcgcccgg cctggatgtg cgggtgttgg cgcagacttc gaagaagcgg acggcaccgc
    10621 tggccgacat cgtggccggc gccgacctgg tggtgacttc atacacactc cttcgtctgg
    10681 acgaggagga atacgtcggg ttgccctgga gcgtggcgct gctggacgag gcccaattcg
    10741 tgaagaaccg gcagtcgcgc ggctaccagt cggtgcgcaa gttgcgggcg cggacgaagt
    10801 tcgcaatcac cggcacgccg ttggagaaca acctgatgga cctctggtcg cttctctcga
    10861 ttgtcgcgcc ggggctgttc gccgatccgg tggcgttcgc ggagcgatat cggcgtccga
    10921 tcgagaacgg gtcggatccg gccgcgctgg gtcggctgca ccggcgggtg cgtccgctga
    10981 tgttgcggcg gacgaaggag acggtggcgt ccgagctgcc gccgaagcag gagcaggtgc
    11041 tctcggtcga actctcgccg gcgcaccggc ggctctacga caagcagttg gcggccgagc
    11101 gcaagcaggt gctcgggctg gtggacgacc tgaaacggaa tcggatcacg attctggctt
    11161 cgctcacccg gttgcggcag cttgcgctgt cgccggcgtt ggtgctgccg gggcacgggg
    11221 cggcgtccgc gaagatcgac acgctgatcg agctggtcac cgagctggcg gccgagggcc
    11281 atcgcgcgct ggtcttcagc cagttcacca cctacctggg gatggtgcgc gagcgtctgg
    11341 ccgaggaggg gatcggtacc agctacctgg acggccggac gcgcgatcgt ccggctcgga
    11401 tcgctgagtt ccgcgaaggg acgaacccgg tgttcctgat cagcctgaag gcggggggtt
    11461 tcggcctcac gctgaccgag gccgactacg tcttcatcct ggacccctgg tggaacccag
    11521 ccgcggagtt gcaggcgatc gaccggaccc accggatcgg ccaggaccag ccggtgatgg
    11581 tctaccgcct ggtcagtgct gacacgatcg aggagaaggt ggtcgctctg caggagcgca
    11641 aacgcgacct gttcgccaag gtggtggggg aggccggcga cctcgccgcc ccgctgtccg
    11701 ccgaagacat ccgcggcctg ctcgagccct gatcgaatca gctcggaccc ttcgcgatcg
    11761 ttgtcggtag tctcgtcgtc gtgacgagcc gagcagcgta cttggccgca ttcgaggcct
    11821 accgcagggc gctcgaggcg gagggcttcg cagtcagtga cgtgagaacg gacgagcggg
    11881 gattgctcac ctggggcgcc ggcgacgcgg aatcgcctgc ggaggtcgcc cgcttggatg
    11941 agatccacga gcagcacttc aagacggccg agcgcgagta cgtccgggag gtcttcgacc
    12001 ggatctacgg cacagatcgg catggctcgg tttgacccgt gagtctccga caccgaaacg
    12061 gtccctgagg agccgtccgg tgaggaacga accggacggc gtcacgaagc gccgaggtaa
    12121 acccaccttt agccggttat gggacgccga aaggttgccg ggtttcgtgc aacccacttt
    12181 tgactggttg tcagccctga attgcctgcc acaaccggta gaaagtgggt tgtgtccgcg
    12241 ggcggcgcca gtaggggagt cataaccggc caaaaccggg ttgcctgttg gcctcagtcg
    12301 tggtgaaccg gcgtgggcac gcccagggct tcgagcatcg ggagcagctt ggcctcggtt
    12361 tcggcgtact cctcggccgg gtcggactcg gacacgatgc cgcagccggc gtagatcgtg
    12421 atccggcgcg ggtcggacgg gtccagcagt ccgcagcgca aggcgatcgc gaactcgccg
    12481 tcaccgttcg cgtccagcca gccgaccggg ccggagtatc gtccccgatc caggtgctcc
    12541 aactcggcga tcactttgcg cgccacctcg gtcggggtgc cacagaccgc tgcggacggg
    12601 tgcaactcgc cggccagccg tagcgcactc acgtcgggat gtgcgaccgc cgtcacatcg
    12661 gaagccaggt gcagcacgtt cggcagttcc agcacatagg gcgcgtccgg cacgttcatt
    12721 cccgagcaga acggcgccag tgcccgagct accgactcga ccgccaactc gtgctcgatc
    12781 aggttcttcc ccgaccgggc caaggcgtgc gcgagcttga ggtccaactc ctccgcaccg
    12841 ttccgccgaa tcgtcccggc cagcacccgc gaagtggcca gaccgtcctc gcgacggatc
    12901 agcatctccg gggaagcccc gaccagtccg tcaacgtagt acgtccagca gctcgggtag
    12961 cggtcggcca actcgcgtac cagggtgcgt ccgtccaacg gctcgtcggc ctcaagggtc
    13021 actccgcggg ccagcaccac cttgtcgatc tcgccctggc cgatacgccc gaccgcctgc
    13081 tcgacgatgt cctgccactg ctcgcgatcg aggctctctg ccaccttgcg gacggactgt
    13141 gaagcggtgt gggcggcggc ggtctcggca tgctcaaccg gccggggatt gtcgggctcg
    13201 accggctcag cggtggcgtc cggtgcaagc ccgtcgctga tctcggtcaa ccagctgcag
    13261 cccttgcggc gtccgatcag caccctgggc acgaccagca ccgactgctg tgcggtcgtc
    13321 gccggatcga acacgaagga cgcgaacgcc accgggccga ccgggccgca ctccagttgc
    13381 agcgcgccgc cgtcgagctg agcgcagaac tcctgccacc aggcttccgc ctcggtgaac
    13441 gtgccggaga agcgcgcgac ctcgccgacg ccgaccattc cgtcgccgcg gcgtagccac
    13501 gccacgccct cggtgcgcag gtacgcaaga aggttgccca cctcatcgat ggcccggctg
    13561 ctggcacgca gacgcgtcct cggcgggtgc tgaatcacca acgcaggata gccgttgcgc
    13621 aacccttgac cagacggggc gccaaatggc tccgaacgcg cattttccct agactggccc
    13681 gagtcccagc agccaccacg caggaggacc catgtccgag ccagagccgg cgattccggc
    13741 taccggctca cctgtcgaag ccgacgtgat cgtggtcggc gccggcccag gtggctccgc
    13801 tgcagccgcg cacctcgccg gtcgcggcct cgacgtcgtg ttgctggaga agagcaagtt
    13861 cccgcgcgaa aaggtctgcg gcgacggcct cactccccgc gcagtgaagc agctgatcaa
    13921 gctgggcatc gacaccagcg aggaagccgg ctgggtgcac aaccgcggcc tacgggtcta
    13981 cgggggacgc accgagccgt tcgagctgcc ctggcccgac ctcgccgagt tcccgcccta
    14041 cggcctggtg cgtgcccgcg ccactttcga caagatcctc gccgaccgcg cggtcgccgg
    14101 cggcgcccgg ctgtacgagc agaccaacgt caccgcgccg atcatcaacg agcgcaccga
    14161 ccgcatcgtc ggcgtccaga gcaaggacgg ccgcaccttc cgcgcacccg tcgtgatcgc
    14221 agcggacggc aactccgccc gcctcgcact cggcatgggg ctcgccaagc ggactgaccg
    14281 gccgatgggc gtagccgtcc gcgcgtactt caactcgccg gcccgcacca acgacgacca
    14341 catggagtcg tggctcgaac tctgggacgg caaaccaggc gaatccagcc tgctgcccgg
    14401 ctacggctgg atcttcggca tgggcgatgg gacggtcaac gtcggcctcg gcacggtcag
    14461 ttccagctcc gagccggcga agatgaatta ccgcgagatg ttctcgcgat ggctggcgaa
    14521 cacaccggcg gagtggggct tcaccccgga gaaccaggtc gggccgacgc tcggcgccgc
    14581 cctgccgatg agcttcaacc gtcagcccgc ctacgcccgc gggctgctgc tggtcggcga
    14641 ctccggcggc atgatctccc cgttcaacgg tgaaggcatc tcctacgcga tggaagccgc
    14701 cgaaatggcc gcggacgcga tcgcggacgc ccggttccgc ggcttcggta cgccgagcgc
    14761 agagactgcg ctgcgcggct acccggccag gttgaaggcc gaatggggcg ggtacttccg
    14821 gctgggacag gtcttcgtgt cgctgatcga gcatccgcag gtgatgcacc tgtgcacccg
    14881 ctacgggttg ccgcggccga ccttgatgag attcaccatg aaactgcttg cacacctgta
    14941 cgacacgaaa gatggtgatt ggatggacaa agtcatccga ggcctcgcaa aggtggcacc
    15001 ctcagcatga caaccaaacc gcagtgctcg ccgcagaagg gacaaagatc ctgatgagcc
    15061 cctatatccc gttgctgggc atcatggcgc tggccgcagg ctttcttgcg atctccctgc
    15121 tgctgagcgc cttcgtcggg ccgcgccgct acaacaaggt caagtactcc tcgtacgagt
    15181 gcggcatcga acccaccccg cagccggccg gcggcggtcg gttcccgatc aagtactaca
    15241 tcatcgcgat gctgttcatc gtgttcgata tcgagactat cttcctttac ccgtgggcgg
    15301 tcaccttcaa gcagctgggc ctgttcgccc tgctggagat ggtgctgttc atggcgaccg
    15361 tgttcgtcgc gtacgcctat gtctggcgtc gtggcggcct ggaatgggac tgagaaggcg
    15421 taggggccga tatgggtatt gaagacaaga tcccccaggg cgtggtgctg actacggtgg
    15481 agggcatttt cgggtggatg cgacaggcgt ccttctggcc ggccaccttc ggccttgcct
    15541 gctgtgccat cgagatgatg gcctacggca cgccgcgctt cgacgccggc cgctggggcc
    15601 aggaggtctt ccgggcatca ccccgccagg ccgacctgat gatcatctcg ggccgggtca
    15661 gccagaagat ggccccggtg ctgcggcagg tgtacgacca gatgccgaac cccaagtggg
    15721 tcctggcgat gggcgtatgc gcctcttcgg gcgggatgtt caacaactac gcgatcgtcc
    15781 agggcggcga ccacctggtt ccggtggaca tgtatgttcc cggctgcccg ccgcgtccgg
    15841 acatgctgat cgacgccatg tggaagctgc gcaaggacat cgtcgccaag cgtccgatcg
    15901 gccagaacga gctggacgct tacgaggcgg ccgagaccgc agcgctcacg gcccccgcca
    15961 cccacgagat gaagggcctc atgcgatgag tgatcccaag ctccccggcg aggagaagag
    16021 catcgacaag ccggtgatgg atccggaggc ttcgcccaag gccggcgtgg agcgcgagga
    16081 cgcccagccc gacccggagc tcaccccggc gatcatcgag gcgcccaagc cgatcgcggt
    16141 gaagaagggc atgtggggtt cgggcaccgg cgacacgtcc ggctacggcc gcattgtcaa
    16201 gcccgtcgag tggcccgctc cgagcgcgcc gccgtacggc tcgtggttcg acggtgttca
    16261 cgaccggctg gccgccctgg tggccgaccc gttcatcaag gtcgtcctcg accgtggcga
    16321 actcaccttc cacatcccgc gggcaaagct cgtcgaggtg atgcaggcgc tgcgggacga
    16381 cgcgcagctg cgcttcgagt tctgcgcctc cgtctccggc gtgcactacc cgcaggccgc
    16441 cggtgaggaa ctgcacgtcg tctaccacct gcagtcgatg acccacaatc gccggctgcg
    16501 cgtggagacc tcgtgtcccg aggccgatgc gcacgtgccc agcatcgtcg cggtctaccc
    16561 agcggccgac tggcacgagc gcgaagcctg ggacatgttc ggcatcatct tcgacggcca
    16621 cccgcacctc acccgcatcc tgatgccgga cgactgggta ggccacccgc agcgcaagga
    16681 ctacccgctc ggcgggatcc cgatcgaata caagggcacg accgttccgc cggtcgacca
    16741 gcgcaggagc tactgatgag cgcccagcac accgactact acgcggctcc cggtgaggag
    16801 accgaaggca ccgtctacac cgccatgggt ggcggcgact ggggcgacat cgccagcgag
    16861 caggccgagc gcggcgacga gaccctggtc atcaacatgg gcccgcagca cccgtccacg
    16921 cacggcgtgc tgcgcctgat ggtggagtgc gacggcgaga cggtgcagag catccgtccc
    16981 ggcatcggct tcctgcacac cggcatcgag aagaacatgg agtaccggtc ctgga
//
LOCUS       DAOPRQ010000056        17014 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_2834_length_17014_cov_13.695560, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000056 DAOPRQ010000000
VERSION     DAOPRQ010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 17014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17014
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2834_length_17014_cov_13.695560"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            268..1239
                     /locus_tag="PKJ61_08325"
     CDS             268..1239
                     /locus_tag="PKJ61_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013160075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HOC13871.1"
                     /translation="MRIVSVTEPGGPENLFITEAAPPFAAPGEVLVAVHAAGVNRADL
                     LQRQGFYPPPPGASDIIGLEVAGAIVELGEGVHGWAIGDECVALLAGGGYAEYVNVPA
                     GQVIRPPAGIDLISAGGLIEAAATVLSNFDHVHLKAGETVLIHGGTGGVGTFAIQYAK
                     AIGARVLTTAGSAEKLELCRELGADEAFDYHDDWVAAVAETTAGKGVDVILDVIGAKY
                     LESNVASLALDGRLVVIGLQGGRKATLDLNALLVKRATVTATSLRGRPVAQKAAIAAA
                     VAERVWPLVTAGAIKPPRETRFRLADVAQAHALLASGENIGKVILTM"
     gene            1339..>2423
                     /locus_tag="PKJ61_08330"
     CDS             1339..>2423
                     /locus_tag="PKJ61_08330"
                     /inference="COORDINATES: protein motif:HMM:TIGR02050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HOC13872.1"
                     /translation="MRVDFAPSKQSSVGIEWELGLVDLASGELTPAAESVLEACGGGE
                     TEPIRKEFLKNMIELVSGAHTTVGEAVADLSANLLIVQRVIGPMGIGLIGSGSHPFSK
                     SDAQERFTGSRYDVVADLNQYWGRQMAICGTHVHVGVDHKDKALPITNGLARFYPYLL
                     AVTCSSPYWEGADSGYASQRTMIFQQLPTNGLPYPMSRWSEFEEYAEELLDSHMISVA
                     AEIRWDVRPSPKFGTVENRTGDSVPTLAEIGCLAALTQCFAEHISRGYARDHTYESLP
                     PWLVRENKWRAARYGMDAEVITPNREKREVPLREGLLNWLERLTPIARDLGCETELHF
                     AAELAERGSSYVRQREAGGPEAALRLLL"
     assembly_gap    2424..2523
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2700..3428
                     /locus_tag="PKJ61_08335"
     CDS             2700..3428
                     /locus_tag="PKJ61_08335"
                     /inference="COORDINATES: protein motif:HMM:NF005743.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HOC13873.1"
                     /translation="MLPFLLLATRDHDTAAAAEYQSVLRHTGLDASELVHLRVETQPL
                     PPLDLSEYSGVILGGSSFNISDEIKSSIQQRVEADLRGLVDQIVDADYPFLGMCYGVG
                     TVSTHLGGRVDREFGEAVGAIEVTQTEAGLSDPLLAGMSPLFHAFVGHKEACNGAPPG
                     IELLATGVDCPVQMYRVGMNVYVTQFHPELDADDLAARMRIYQHAGYFAPSELDDLIT
                     MAANSQVNGEQHRVLRNFVERYAR"
     gene            complement(3551..4279)
                     /locus_tag="PKJ61_08340"
     CDS             complement(3551..4279)
                     /locus_tag="PKJ61_08340"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOC13874.1"
                     /translation="MAEFNLQRLEDDGFKRVLCVVAHPDDIEYGTSAAVAAWTARGIE
                     VCYLLLTGGEAGMQRPPEQTRVLRAAEQREASEVVGVQDLTILDHPDGMLVYSLDIRR
                     DIARHIRKIRPDVVVTGSWEVEFVAGLNQADHRVAGLACVDAIRDADNTWVFPELAAD
                     EGLPKWGVSWLLVAGDSRPTHAVDVSGRPLERGILALEAHGDYLGGLSGHPLPRDLLT
                     EMTTAQGAALGVPNAVAFRAYKLR"
     gene            complement(4461..5471)
                     /locus_tag="PKJ61_08345"
     CDS             complement(4461..5471)
                     /locus_tag="PKJ61_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family protein"
                     /protein_id="HOC13875.1"
                     /translation="MKFRYLGNSGLKVSEICYGNWLTHGSQVENETATKCVRAALESG
                     ITSFDTADVYANTVAESVLGKALKGERRESLEIFTKVYWPTGPKGPNDCGLSRKHIME
                     SIHGSLKRLKTDYVDLYQAHRYDHETPLEETMQAFADVVRQGKALYIGVSEWDANQIR
                     AGHALAKDLGIQLVSNQPQYSMLWRVIEEWVVPTSEELGVSQICWSPVAQGVLTGKYL
                     PGQPLPKNSRATDENGGAAMIQRFLRDDVIETVQKLRPIAEELDLTMAQLAVAWVLAN
                     PNVACAIIGASRPSQIKENVAASGVQLPDEVLSRIDDALGDVVVRNPMLTAENTPKVR
                     PC"
     gene            complement(5468..6529)
                     /locus_tag="PKJ61_08350"
     CDS             complement(5468..6529)
                     /locus_tag="PKJ61_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13876.1"
                     /translation="MTQQNPGFRTVMRGYDPNEVDRSLTDLRKALDQARAEAADGSVA
                     VTKLNSQVAQLGEQANSYRARIASLEQEQKEAAAPSFTDLGARIGKMLKLAEDEAAEL
                     RAKAQADADRVTAEATAAAEQATTAADRYATDVTTKADAEAARVLEAAKQQADEIIDY
                     ADREATARREEAEAVYEHQRARAAAAAADFEKTLAERRDKAAQEFAVQMQNNEQAIAA
                     AQERQAAIEAEADRTLAEAKAQADAHIKTARDEAAQHVDSSRLAAEKIRRESERELQA
                     ATARRDAITAQLTNVRQMLATLGGAAIVNPLAEPEAAPVAAAEAPVEAAEEATVVEEP
                     TVAAEAPADVAPTEEEEQA"
     gene            complement(6526..7959)
                     /locus_tag="PKJ61_08355"
     CDS             complement(6526..7959)
                     /locus_tag="PKJ61_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13877.1"
                     /translation="MLDPAVRPPAQPAGSELTRQANEILTQAQNLADQLRTEAQAEAD
                     AVRAETAKNRELSEQLAREVAEHRAAAEEAMTSAATRLEEARQDSLTLLSDATEQASM
                     ITGTAERTAAEVTSNARAEAEQRIADAQARAAAMLEEAEQVAANGRQQLADERAQVEA
                     ELERRREELAAADAHRREQTDAELAALHSGVISELQRFRTDSQNAVDQRWAEFNAERE
                     RFTAEAEKSRADAHAEREWLRAEAQAKADEQLAAAEAHVNWTQEAMAGLRASAEAEVE
                     ALKRQTHQELAEHARGVREQVGLAATSTAHRHQSLIAEATASAEDLTARAKAVLDAAE
                     RDAANTSQRAQEEAERLVNHAKSEAQAQLERAERRLAEAEAGAKLIRERSASDLEKLQ
                     RETLAKTKASREEAVTLLTEARAEADSVRGEARSLMERARAEVAALARRRDDINAQLG
                     HLSGVIEALAVSERPGQTPNQVTEEES"
     gene            8097..9527
                     /gene="thrC"
                     /locus_tag="PKJ61_08360"
     CDS             8097..9527
                     /gene="thrC"
                     /locus_tag="PKJ61_08360"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016667876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOC13878.1"
                     /translation="MQYLSTRDPAGATLGFTDILLEGLAPDGGLYLPESYPQVDGARL
                     ASWRALLASEGYAALAHAVLSLFIDDIPSADLRELCARAYTDSVFADPQVVPVTELDG
                     GLWLAHLSGGPTAAFKDMAMQLLGQLFEYELARRGAELNILGATSGDTGSAAEYAMLG
                     RTGIRVFMLTPAGRMSPFQQAQMFSLDDPAIVNIAVTGVFDDCQDLVKAVASDLEFKA
                     RYSLGAVNSINWARLVAQVVYYIASWLRVTSTDDELVSFCVPTGNFGNIMAGHVARMM
                     GLPIDRLVLATNENNVLDEFFRTGLYRVRSAAETHETSSPSMDISKASNFERFIFDLL
                     GRDAARVRELFAVVLPRDGYFDLSGTPEFASLQQKYGFVSAASTHADRVATIRSLWET
                     SGVLIDPHTADGVLVARDFVRPGVPMIVTETALPVKFAATIVEAIGREPDRPARFDGL
                     ESLPRHVIDLPNDADALKALIAARVR"
     gene            complement(9654..11726)
                     /locus_tag="PKJ61_08365"
     CDS             complement(9654..11726)
                     /locus_tag="PKJ61_08365"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HOC13879.1"
                     /translation="MPLDNTNPFTSASTLPYELPDFTRIGDEHYQPAIEASMAEQLEA
                     LDALTANPEPPTVANVIEAWETSGQMLSRALNAFYTKQPADTNDTLDAVEAAIAPQLA
                     AHRDAIFLSRSRYERVAALAKRVAAGEVEVDPQSAYWLERCLRDFERAGVNLPDPEQQ
                     RLRELNQRIAELQSAFGRLALAGMNAGAVLVTDRAELDGLSDAEIEGARERAAEQGHA
                     DAWLLELDNTSGQRPLDVLKHRGLRERLFKASIGRGLGGGHDTRPIIVELARLRAERA
                     ALLGFANHADYVAADGCARGGAAVWAMLTKLAPAAVANARKEADEMAEVWRRIEPEGP
                     LAAWDWQYVSELLRAERYQLDASALRPYLELERVLNEGVFAAANGLYGITFAERADLR
                     GYNDEVRVFEVNDADSTPIGLFVADFYTRPGKQGGAWMNNLVDQNHLLGQLPVVCNNS
                     NLIKPPAGQPTLMTWDDVITLFHEFGHALHGLLSDVRYRSQSGTETPRDFVEYPSQVN
                     EMWALDAEILPRFAVHHATGEPMPAEWIETLRASTRFGQGHKTTEYLGAALLDQVWHS
                     TPLDELPTGPEHVEAFEQAALAKVGLDYELVPPRYRSGYFRHVFEGGYDAGYYAYIWS
                     EVLDADTAAFIRANGGLTRENGDRFRRRLLARGGSVDAMDAFRDYRGQDPDLQHLLDR
                     RGLATEVG"
     assembly_gap    12001..12100
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(12106..12513)
                     /locus_tag="PKJ61_08370"
     CDS             complement(12106..12513)
                     /locus_tag="PKJ61_08370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13880.1"
                     /translation="MPKSTPNRPAVGGGSLRARARLLAVVLLTMLTVLFAVMGLRLYL
                     APPVDALSGSLYGFVALMTALAYGLSARWVAIQRRWGHLVALAVVVLGAVLGLTANMT
                     WLEWAMLGANVLALACLAASIPRKSATTSPRKA"
     gene            12572..13189
                     /locus_tag="PKJ61_08375"
     CDS             12572..13189
                     /locus_tag="PKJ61_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13881.1"
                     /translation="MSFSAVELVGYLASALVVASLAMTSVVRLRTVSLIGSLVFVAYG
                     ALLPSIPIIITNAAVAGLNIWFLRKEFGAHRDLGAVPIEPRAPFLVDFLSSHTDDIAS
                     FHPKFRLPEAGDFALVLTRDGLPAGALVGRPDADTLQLQLDYVMSAYRDSRIGDWLYG
                     PGAKALTDAGFSRVVAQPESAALRTYLLRVGFTETDAGLVRQLRP"
     gene            13220..13657
                     /locus_tag="PKJ61_08380"
     CDS             13220..13657
                     /locus_tag="PKJ61_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012922795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13882.1"
                     /translation="MTLFGLPLHPLVVHAAVVLLPLAAFGAIVIALSARVRQRFGWLV
                     FAGAFVAGGSVAAARITGETFAGGTTGTGTLATHMTWGLLAPWPATALAVSALLLMLT
                     NKATSRPLKITVGALTIVSAVAALAVIVVVGHSGASSVWGAPA"
     gene            complement(13720..14379)
                     /locus_tag="PKJ61_08385"
     CDS             complement(13720..14379)
                     /locus_tag="PKJ61_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13883.1"
                     /translation="MSLRRFLALPAVLLLAACATVNPSAPATTPTETAPSSAQPSATV
                     SATPTVAETSASPSVSPSQAPAASLVLRGDGLGTFNFGSNQAPVAELLSDQLGAPDET
                     SQGILCELDDSSPWAETRTFGGLWVQFVAADTHKSSPRTLAAWGFQLSEEFAAPLQLA
                     DDVPLTLSFKELKAKYPKGKLEDIGFGDGSKMFTLPNQMRFIGAGKPEMVQAGELSFC
                     E"
     gene            14507..15634
                     /locus_tag="PKJ61_08390"
     CDS             14507..15634
                     /locus_tag="PKJ61_08390"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008598546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HOC13884.1"
                     /translation="MAQEKKRIGILTAGGDSPGLNAAIRGFGKAAIGNGWELLGYRDG
                     IRGLVENRYSLLDPKALSGILTIGGTVLGTSRDKVHRMVVDGEVRDMRDQVVANYEKE
                     GLSGLVLLGGGGTAKNAMRLAQAGLNVITLPKTIDNDIARTDTTFGFATAMEIATDAI
                     DRLHSTAHSHHRIILTEIMGHRAGWLTLGAGIAGGADIILLPEIPYSVDSVADTIKQR
                     LQKGSTFSVVAVAEGARDRHDMADYEAAQLLVKAAKSPESARAAKAHLANVEDAQREH
                     TFKLARELEAATGLESRVSILGYVQRGGTPCAADRLLASRLGTAAAHAVSKGQFGVMV
                     AARGEDTELVPLEEVAGQVKLVPTDHEWIATARKLGTGLGD"
     gene            15636..16457
                     /locus_tag="PKJ61_08395"
     CDS             15636..16457
                     /locus_tag="PKJ61_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017935179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HOC13885.1"
                     /translation="MQRRFAQIDVFGDGGITGNPVAVVLDGSGLSDARLQAFASWTNL
                     SETTFLFPPTDPAADYRVRIFTASAELPFAGHPTLGSAHAWLEAGGRPQRPGVVMQEC
                     GVGLVGVRLDNARLAFAAPPRRRSGPLDEATITLAEMVLGIPREQWVAHEWGDNGAPW
                     MMVQLADADAVRSVRPNHSHLGATQHLGVIGLASDQFAYEVRGFVGDWEDPVTGSLNA
                     AAAQWLRGRGLVPSEYVAAQGSQLSRSGEVFINDDGTRIWVGGRVRTVITGSVDL"
     gene            16627..>17014
                     /locus_tag="PKJ61_08400"
     CDS             16627..>17014
                     /locus_tag="PKJ61_08400"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HOC13886.1"
                     /translation="MPALRSRTTTHGRNMAGARALWRATGMVESDFGKPIVAIANSFT
                     QFVPGHVHLKDMGQLVAGAVAEAGGVGREFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADALICISNCDKITPGM"
BASE COUNT         2539 a         5845 c         5625 g         2805 t
ORIGIN      
        1 cgttaagctc ggccgctttc aaggcggctg cactagtcca ctatgcgacc tcgccagtcg
       61 acccatcata acgattcatg ctgtgcgtag gatcgattcc acctagttcg gcccgctgac
      121 tacggcggac gctgctcttc tccgctcctt ccactgggtt ccgcccccgc tggcgcgggc
      181 gaggggattg agcacagacg cgaaccgaca acggctaacc cggctggtga cattcacctg
      241 gacggggcca gatgcaaagc tgggggcatg cgaatcgtct ccgtaaccga gcccggcggt
      301 cccgagaacc tgttcatcac cgaggcggcc ccgccgttcg ccgcgcccgg cgaggtgctc
      361 gtcgccgtcc acgccgccgg cgtgaaccgg gcggacctgc tgcaacggca gggcttctat
      421 ccgccgccgc ctggcgccag cgacatcatc ggccttgagg tcgccggcgc catcgtcgag
      481 ctcggcgaag gcgtccacgg ctgggcgatc ggtgacgagt gcgtagccct gctggccggc
      541 ggtggctacg ccgaatacgt gaacgtcccg gccggacagg tgatccggcc gcccgccggc
      601 atcgacctga tcagcgccgg cgggttgatc gaggcggccg cgaccgtcct gtcgaacttc
      661 gaccacgtgc acctgaaagc cggcgagacc gtactgatcc acggcggcac cggaggcgtc
      721 ggcacgttcg cgatccagta tgcgaaggcg atcggcgccc gcgtgctcac caccgccgga
      781 tcggcggaga agctcgagct gtgccgcgaa ctcggcgcgg acgaggcctt cgactaccac
      841 gacgactggg tggccgcggt cgccgaaacc accgccggca agggcgtgga cgtgatcctg
      901 gacgtaatcg gcgccaagta cctggagtcg aacgtcgcct cgctggccct ggacggacgc
      961 ctggtagtga tcggcctcca ggggggacgc aaagccaccc tcgacctgaa cgcactgctg
     1021 gtgaagcggg ccaccgtgac cgccaccagc ctgcgcggac gtccggtcgc gcagaaggcc
     1081 gccatcgccg cagccgttgc cgagcgggtg tggccgttgg tgaccgccgg cgcgatcaag
     1141 ccgccacgcg agacccgctt ccgcttggcg gacgtcgccc aggcgcacgc cctgctcgcg
     1201 tccggggaga acatcggcaa ggtgatcctg acgatgtagg cgccgggctc aacccagctt
     1261 cggcccgcgt ccgagcagcc atttgaagcg atggggtacc tgtctagaat caggcccagt
     1321 ctcagtgagg atccgagcat gcgcgtcgac ttcgccccgt ccaaacaatc gagcgtgggc
     1381 atcgaatggg agctcggcct ggtcgacctg gcctccggcg agctcacccc ggcggccgaa
     1441 tccgtcctcg aagcttgcgg cggcggtgag accgagccga tccgcaagga attcctgaag
     1501 aacatgatcg agttggtctc cggcgcgcac accaccgtgg gcgaggcggt cgccgacctg
     1561 tccgccaacc tgctgatcgt gcagcgtgtc atcgggccga tgggcatcgg cctgatcggg
     1621 tcgggctcgc accccttcag caagtcggac gcgcaggagc gcttcaccgg ctcccgctat
     1681 gacgtggtgg ccgacctcaa ccagtactgg ggacgccaga tggccatctg cggcacccac
     1741 gtccacgtcg gcgtcgacca caaggacaag gcactgccga tcaccaacgg cctggctcgc
     1801 ttctacccct acctgctggc ggtcacctgc tcgtcgccgt actgggaggg cgccgattcc
     1861 ggctatgcgt cccagcggac gatgatcttc cagcagctgc caaccaacgg gctgccctac
     1921 ccgatgagcc gctggtcgga gttcgaggag tacgccgagg aactacttga ttcccacatg
     1981 atcagtgtcg cggccgagat tcgctgggac gtccgaccgt ccccgaagtt cggcacagtc
     2041 gagaatcgga cgggcgactc ggtgccgacg ctggccgaga tcggctgcct ggccgcgctg
     2101 acccagtgct tcgccgagca tatctcccgc ggctacgccc gcgaccacac ctacgagtcg
     2161 ttaccgccgt ggctcgtgcg cgagaacaag tggcgtgcgg cgcgctacgg catggacgcg
     2221 gaagtgatca cccccaatcg ggagaagcgc gaggtgccgc tgcgcgaggg cctgttgaac
     2281 tggctggaac ggctcactcc gatcgcacga gacctcggct gcgagaccga gttgcacttc
     2341 gcggccgagc tcgccgagcg ggggtccagc tacgtccggc agcgggaagc cggcggaccg
     2401 gaggccgcgc tgcgcctgct gctnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnngctacgt ccggcagcgg gaagccggcg gaccggaggc cgcgctgcgc ctgctgctgg
     2581 ctgagaccgg ggcagcgtcc ccgttcacta cctagcatca ggccaagatc caccttcgct
     2641 cgagtcggcg gacgcgggcg ctccggcagg tcgagcccgt gccgcctatc atgacttccg
     2701 tgctgccctt cctgctcctt gccacccgcg accacgacac cgccgccgcg gccgagtacc
     2761 agtcggtact gcggcacacc gggctggacg cgtccgaact ggtccacctt cgggtcgaaa
     2821 cgcagccgtt accgccgctc gacctgagcg agtactcggg cgtaatcctg ggcgggagct
     2881 ccttcaatat ctcggacgag atcaagtcgt cgatccagca gcgggtcgag gccgacctgc
     2941 gtgggctggt ggatcagatc gtcgacgccg actacccgtt cctcggcatg tgctacggcg
     3001 tcgggacggt gagcacgcac ctcggcggcc gggtcgaccg cgagttcggc gaagcggtcg
     3061 gcgcgatcga ggtgacccag accgaagccg gactgagcga tccgctgctg gccgggatga
     3121 gcccactctt ccacgcgttc gtcggccaca aggaggcctg caacggcgcg ccgcccggga
     3181 tcgaactgct ggccaccggg gtggactgcc cagtgcagat gtaccgggtc gggatgaacg
     3241 tgtacgtcac ccagttccac cccgaactgg acgccgacga cctcgccgcc cggatgcgca
     3301 tctaccagca cgccggctac ttcgccccgt ccgagctgga cgacctgatc accatggcag
     3361 ccaacagcca ggtgaacggc gagcagcatc gcgtgctccg caacttcgtt gagcggtacg
     3421 cccgctgacg atccctgagc ctctccaacg atccctgagc ttgacgaagg gtccgtctca
     3481 ctgcaaccgg gtggctgcgg cccttcgtga cgcgctcgtt cctcacgctc ctcagggacc
     3541 gctttggcag ctagcggagc ttgtaggccc ggaacgccac cgcgttggga acgccgagcg
     3601 ccgcaccctg ggcggtggtc atctccgtga gcaggtcccg gggcagcggg tggcccgaca
     3661 gcccgcccag atagtcaccg tgggcttcga gcgcgaggat gccacgctcg agcgggcgtc
     3721 cgctcacgtc caccgcatgg gtcggacggc tgtcgccggc gaccagcaac cagctcactc
     3781 cccacttggg aagcccctcg tcagcggcca gttccgggaa gacccaggtg ttgtcggcgt
     3841 cccggatcgc gtccacgcag gccaagccgg ccacgcggtg atcggcctgg ttcaggccgg
     3901 ccacgaactc cacctcccag gagcccgtaa ccaccacgtc cggccggatc ttgcgaatgt
     3961 gtcgcgcgat gtcgcggcgg atgtccaggg agtagaccag catcccgtcc gggtggtcga
     4021 ggatggtcag gtcctgcacc cccaccacct cgctggcctc ccgctgctcg gcggcgcgca
     4081 ggactcgggt ctgctcgggc ggacgctgca tgccggcctc gccaccggtg agcagcaggt
     4141 aacagacctc gatgccgcgg gctgtccatg cggccaccgc ggctgaggtg ccgtactcga
     4201 tgtcatccgg gtgcgccacc acgcagagca cccgcttgaa accgtcgtct tcaagtcgct
     4261 gcaggttgaa ctcggccata ggcccaaggt acgtcgcgtc cgtccggctc aacaggagga
     4321 cttctgccca cgggcccggc cgccaccgtc caccccgtac ccccgtctcg cccgtcccac
     4381 ccgaacacgt tcgataagtg gacggggtca gcccacgggg ccagccgacg ggagcagacc
     4441 cggcggacag gatcagccga tcagcaggga cgcaccttcg gggtgttctc cgcggtcagc
     4501 atcgggttgc gcaccaccac atcgccgagc gcgtcgtcga tacggctgag cacctcgtcg
     4561 gggagctgga cgccgctggc ggccacgttc tccttgatct ggctcggccg ggacgcaccg
     4621 atgatcgcgc acgcgacgtt cggattcgcc agcacccagg ccaccgccag ttgtgccatg
     4681 gtgagatcga gctcctcagc gatcggacgc agcttctgca cggtctcgat cacgtcgtcg
     4741 cgcaggaagc gctggatcat cgccgcgccg ccgttctcgt ccgtggcccg cgagttcttg
     4801 ggcagcggct ggccgggcag gtacttgccg gtgaggacgc cctgggcgac cggtgaccag
     4861 cagatctggg aaacgcccag ttcctcggaa gtcggcacga cccactcctc gatcacccgc
     4921 cacagcatcg agtactgcgg ctggttcgac accagctgga tgccgagatc cttggccagc
     4981 gcgtgaccgg cgcggatctg gttcgcgtcc cactcactga caccgatgta gagcgccttg
     5041 ccctggcgga cgacgtccgc gaacgcctgc atggtctctt ccagcggcgt ctcgtggtcg
     5101 tagcggtggg cctggtagag gtcgacgtag tcggtcttga gccgcttcag cgagccatgg
     5161 atcgactcca tgatgtgctt gcgcgacagc ccgcaatcgt tcggcccctt cggcccggtg
     5221 ggccagtaga ccttggtgaa gatctccagc gactcccgcc gctcgccctt cagcgccttg
     5281 ccgagcaccg actccgcgac cgtgttggcg tagacatcgg cagtgtcgaa gctcgtgatg
     5341 cccgactcca acgcggcgcg gacgcacttg gttgcggtct cgttctcgac ctgtgaaccg
     5401 tgggtcaacc agttgccgta gcagatctcc gagaccttca ggccggagtt gccgaggtaa
     5461 cgaaacttca tgcctgctcc tcctcctcag ttggggctac gtccgcggga gcttccgcag
     5521 ccaccgttgg ctcctcgacc accgtcgcct cttcggccgc ttccactgga gcttcggcag
     5581 cagccaccgg tgccgcttcg ggctcggcca gcgggttcac gatcgcagcc ccgccgagcg
     5641 tggccagcat ctggcggacg ttggtcagct gcgcggtgat cgcgtcccgg cgcgccgtgg
     5701 ccgcctgcag ttcgcgctcg gattcgcgcc ggatcttctc cgcggccagc cgcgacgaat
     5761 cgacgtgctg cgcggcttcg tcccgagcgg tcttgatgtg cgcatcggcc tgcgccttgg
     5821 cctcggccag cgtccgatcg gcctcagcct cgatcgcggc ctgccgctcc tgggccgcgg
     5881 caatcgcctg ttcgttgttt tgcatctgga cggcgaactc ctgtgcggcc ttgtcgcggc
     5941 gctcggccag ggtcttctcg aagtcggcag ccgcagccgc cgcgcgggca cgctggtgct
     6001 cgtacaccgc ctcggcctcc tcgcggcgag cggtggcctc gcggtcggcg tagtcgatga
     6061 tttcgtcggc ctgctgcttg gccgcctcca ggacgcgggc agcttcggca tccgccttcg
     6121 tggtgacgtc cgtggcgtag cggtcggcag cggtggtcgc ctgctcggcg gcagcggtgg
     6181 cttcggccgt gacgcgatcg gcgtccgcct gtgccttcgc gcgcaactcg gcagcctcgt
     6241 cctccgccag cttcagcatc ttgccgatcc gggcgcccag atcggtgaag gacggcgccg
     6301 cggcctcctt ctgctcctgc tcgagcgagg cgatgcgcgc ccggtagctg ttcgcctgct
     6361 cgccgagctg ggcgacctgc gagttcagct tggtgaccgc caccgagccg tccgcagcct
     6421 ccgcacgggc ctggtcgagt gccttgcgca ggtcagtcag ggagcgatca acctcgttcg
     6481 ggtcgtagcc gcgcatcacc gtccggaacc ccgggttctg ctgggtcatg attcctcttc
     6541 cgtcacttgg ttgggggtct ggccgggacg ctcagagacg gccagcgcct cgatcactcc
     6601 cgaaaggtgc ccgagttggg cattgatgtc atcacggcga cgggccagtg cggccacttc
     6661 cgcacgcgcc cgttccatca gactacgtgc ctcaccgcgg acgctgtcag cctcggcgcg
     6721 ggcctcggtg agcagcgtca ccgcttcctc gcggctggcc ttggtcttgg cgagcgtctc
     6781 gcgctgcagc ttctccagat cggacgcgga ccgctcccgg atgagcttcg cgccggcctc
     6841 ggcttcggcc agccggcgtt ccgcgcgctc cagttgggcc tgggcctcgg acttggcgtg
     6901 gttgacgagg cgttcggcct cctcctgggc gcgctggctg gtgttggccg cgtcccgctc
     6961 agcggcatcg aggacggcct tggcccgggc ggtcagatcc tcggcgctcg cggtggcctc
     7021 ggcgatcagc gactggtgcc ggtgagcggt ggaggtggcg gcgagcccga cctgctcgcg
     7081 gaccccgcgg gcgtgctcgg ccaactcctg gtgggtctgc cgtttgagcg cttccacctc
     7141 ggcctcagcg gacgcgcgca ggccggccat ggcctcctgc gtccagttca cgtgggcttc
     7201 ggccgcagcc agttgctcgt ccgctttcgc ctgggcctct gcccgcagcc actcgcgttc
     7261 ggcgtgggcg tccgctctgg acttctcggc ctccgcggtg aagcgctccc gttcggcgtt
     7321 gaactcggcc cagcgctggt cgaccgcgtt ctgggaatcg gtacggaagc gctgcagttc
     7381 gctaatcacg cccgagtgca gggcggccaa ctcggcgtcc gtctgctcgc ggcgatgggc
     7441 gtccgcggct gccaattcct cgcggcgccg ctccagttcg gcctcgactt gggcgcgctc
     7501 gtcggcaagc tgttggcgac cgttggcggc cacctgctcg gcctcctcga gcatggcagc
     7561 ggcgcgagcc tgggcgtccg cgattcgttg ctcggcctcc gcgcgggcat tcgaagtgac
     7621 ctcggccgcc gtgcgttcgg cggttccggt gatcatggat gcctgctcgg tggcgtcgct
     7681 gagcagggtc aacgaatcct ggcgtgcctc ctcgagccga gtggcggcag acgtcatcgc
     7741 ctcttcggct gccgcccggt gctcggcgac ttcgcgggcg agctgttcgc tcagctcacg
     7801 gttcttcgcc gtttccgcgc gcacggcatc agcctcagct tgggcttcgg tgcgaagctg
     7861 gtcagccagg ttctgagcct gcgtgaggat ctcgttggcc tgtctggtca gctcactccc
     7921 agccggctgg gcgggcggac gcacagcggg atcgagcacc cttgaatgat ctagggccgc
     7981 gttggggaaa gcaacttcgc gaccgggctg gggctgctta tctgtggtga aggtcgagct
     8041 tcactccacg cgtgatcaac caacccggag tccgggcacg tcggtagagt tctcgggtgc
     8101 aatatctctc cactcgcgac cccgccggcg caacgctcgg gttcactgac atcctgctcg
     8161 aggggctggc ccccgacggc ggcctgtacc tgcccgagag ctacccgcag gtggacggag
     8221 cccgattggc ctcgtggcgg gcgctgctgg cgtccgaggg ttacgccgca ctggcgcacg
     8281 cggtgctgtc gctgttcatc gacgacatcc cgtccgctga cctgcgcgaa ctgtgcgcgc
     8341 gggcctacac cgactcggtg ttcgccgatc cgcaggtcgt gccggtcacc gagttggacg
     8401 gcggactgtg gctcgcgcat ctctccggcg gaccgaccgc cgcgttcaag gacatggcca
     8461 tgcagttgct cggccagctc ttcgagtacg agctggcgcg gcgcggcgcc gagctgaaca
     8521 ttctcggcgc gaccagcggc gataccggtt cggccgccga atacgcgatg ctcggccgga
     8581 ctgggatccg ggtgttcatg ctgacccccg ccggccggat gtcgccgttc cagcaggccc
     8641 agatgttctc gctggacgat ccggcgatcg tgaacatcgc cgtgaccgga gtcttcgatg
     8701 actgccagga cctggtgaag gcggtcgcgt ccgatctcga attcaaggct cgctattcgt
     8761 tgggcgcagt gaactcgatc aactgggctc ggctggtcgc ccaggtggtg tactacatcg
     8821 cttcctggct acgggttact tccacggacg atgagctggt ctcgttctgc gttccgaccg
     8881 gcaacttcgg caacatcatg gccggacacg ttgcgcggat gatggggctg ccaatcgatc
     8941 ggctggtgct ggccaccaac gagaacaatg tgctggacga gttcttccga acggggctgt
     9001 accgggtgcg gtcggccgcc gaaacccacg agacctcgtc gccaagcatg gacatctcaa
     9061 aggcatccaa cttcgagcgg ttcatcttcg acctgctcgg acgggacgct gcccgcgtcc
     9121 gggaactgtt cgcggtggtc ctgcctcggg acggctactt cgatctctcg gggacgcccg
     9181 agttcgcgtc cctgcagcaa aagtacggct tcgtctcggc agcatccacg cacgcggacc
     9241 gggtggccac gatccgttcc ctgtgggaga cctcgggtgt gctgatcgac ccgcacactg
     9301 cggacggggt gttggtcgcg cgcgacttcg tgcgcccggg ggtaccgatg atcgtcactg
     9361 agaccgcgct gccggtgaag ttcgccgcga ccatcgtcga agcgatcgga cgcgagcccg
     9421 accgcccggc ccgcttcgac ggtctggagt cattgccccg tcacgtcatc gacctgccga
     9481 acgacgcgga cgccctgaag gccctgatcg ccgcccgcgt ccgctgagcc tcggtcgcgt
     9541 caaggccgcc catcggccgg ttgacctgtc gtcatctccc acaagctgga cacttccagc
     9601 cgacatcggc tcgaactgtc cagcttgcgt gtggtgattg aggcggcccg cgctcagccg
     9661 acctcggtgg ccagcccgcg gcggtccagc aggtgctgca ggtccggatc ctggccgcgg
     9721 tagtcgcgga acgcgtccat ggcatcgacc gaaccgccgc gcgccaacag ccggcgacgg
     9781 aagcgatcgc cgttctccct ggtcaggccg ccgttggcgc gaatgaaggc ggccgtgtcg
     9841 gcgtccagca cctccgacca gatgtaggcg tagtagccgg cgtcatagcc gccctcgaag
     9901 acgtgtcgga agtagccaga gcggtaccgc ggcggcacca actcgtagtc gagtccaacc
     9961 ttggcgagcg cggcctgctc gaacgcctcg acgtgctcgg ggccggtcgg cagctcgtcc
    10021 agcggggtcg agtgccagac ctggtcgagc agggccgcgc ccaggtactc ggtggtcttg
    10081 tggccctgac cgaaccgagt ggacgctcgc agggtctcga tccactcggc cggcatcggc
    10141 tcgccggtgg cgtgatggac cgcgaaccga ggcaggatct cagcgtccag tgcccacatc
    10201 tcgttcacct gggacgggta ttccacgaag tcacgtggcg tttccgtgcc cgactgcgag
    10261 cggtaccgga cgtccgacag cagcccgtgg agcgcgtgcc cgaactcgtg gaacaacgtg
    10321 atcacgtcgt cccaggtcat cagcgtgggc tgcccggccg gcggcttgat caggttcgag
    10381 ttgttgcaga cgaccggcag ttgtccgagc aggtggttct ggtcgaccag gttgttcatc
    10441 caggccccac cctgcttgcc cggacgggtg taaaagtcgg cgacgaacaa cccaatcgga
    10501 gtcgagtccg cgtcgttcac ctcgaacacc cgtacttcat cgttgtaccc gcgcaggtcc
    10561 gcacgctcgg cgaaggtgat gccgtagagc ccgttggccg cagcgaacac cccctcgttc
    10621 agcacccgct ccaactccag gtacggccgc aacgcggacg cgtccagctg gtagcgctca
    10681 gcgcgcagca actccgacac gtattgccag tcccaggcgg ccaacgggcc ctccggctcg
    10741 atccggcgcc agacctcggc catctcgtcg gcctctttcc gggcgttggc aacggctgcc
    10801 ggcgccagct tggtcagcat cgcccacact gcagcaccac cgcgcgcaca accgtccgcc
    10861 gcgacgtagt cggcgtggtt cgcgaagccc agcagggcgg cgcgctcggc ccgcagccgc
    10921 gccagctcca cgatgatcgg acgggtgtca tgcccgccac ccagcccgcg cccgatcgag
    10981 gccttgaaca gccgctcgcg cagcccgcgg tgcttcaaca cgtcgagggg acgctgcccc
    11041 gaagtgttgt cgagctccag cagccacgcg tcggcgtggc cctgctcggc cgcgcgttcg
    11101 cgggcgccct caatctcggc atcgctcagc ccgtccaact cggcccgatc ggtgaccagg
    11161 accgcgccgg cgttcatccc ggcgagcgcc agccgaccga acgccgactg cagttcggcg
    11221 atgcgctggt tgagctcgcg caggcgctgc tgctcgggat caggcaggtt cacaccggcc
    11281 cgctcgaaat cgcgcaggca ccgctccagc cagtacgcag actgcggatc cacttcgact
    11341 tctccggcgg ccacccgctt ggccagtgct gcgacgcgtt cgtagcggct gcggctgagg
    11401 aagatcgcgt ccctgtgggc agcgagttgc ggggcgatcg ccgcctccac cgcgtcgagg
    11461 gtgtcgttcg tgtcggccgg ctgcttggtg tagaacgcgt tcagcgcgcg gctgagcatc
    11521 tgcccggagg tctcccaagc ctcgatcacg ttcgcgacag tgggtggctc ggggttggcc
    11581 gtgagcgcgt ccagggcttc cagctgctcg gccatactgg cctcgatcgc gggctggtaa
    11641 tgctcgtcac cgatcctggt gaagtcgggc agttcgtacg gcagggtcga cgcggacgtg
    11701 aacgggttgg tgttgtcgag cggcataccg gccatcctta cccggttcgg agtcgctggc
    11761 caagccgaac ggatttgccc ccgagttctc gcgaagggcg cgcacagagc cagttcgggc
    11821 cggacgccgt gcagatccgt tcgccgagtc ggggtgcacc ctcggcgggt gcggacaaaa
    11881 tgccctggat tcggcccaga tcggccaaac cggccgaaaa tcgccgaatc ctgggcattt
    11941 tgtccgacgc tcaggccttt cgcggcgagg tggtggcaga cttccgcggg atcgaggcgg
    12001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gacgctcagg cctttcgcgg
    12121 cgaggtggtg gcagacttcc gcgggatcga ggcggccagg cacgcgagcg caagcacatt
    12181 cgccccgagc atcgcccact ccagccaggt catgttggcg gtgaggccga gcaccgcgcc
    12241 cagcaccacc actgccagcg cgaccagatg cccccagcgc cgctggatcg ccacccagcg
    12301 ggcgctcagg ccgtaggcca gcgcggtcat cagggccacg aacccgtaca gcgagccgct
    12361 caacgcgtcc acgggcggag cgaggtagag ccgcagcccc atcaccgcga acaggaccgt
    12421 gagcatggtg agcaggacga ccgccagcag ccgggcgcgc gcacgcagcg acccgcctcc
    12481 gaccgcaggc cggttgggtg tggatttggg cacgacagaa accgtagcgt tccagcgacg
    12541 gcgaagcgct ggtcgccaat aggctggcgc gatgagtttc tctgcggttg aactggtcgg
    12601 gtacctggcg tcggccctgg tcgtggcttc cctggcgatg acctcggtgg tgcgactgcg
    12661 gacggtctcg ctgatcgggt cgctggtctt cgtggcctac ggcgcgctgc tgccatcgat
    12721 cccgatcatc atcaccaacg ctgcggtcgc cggattgaac atctggttcc tgcgcaagga
    12781 gttcggggcc caccgcgatc tgggcgcagt gccgatcgag ccgcgggcac ccttcctggt
    12841 cgacttcctc agctcgcaca ctgacgacat cgccagcttc cacccgaagt tccgcctacc
    12901 cgaggcgggc gacttcgccc tcgtgctcac ccgggatggc ctgcccgccg gtgcgctggt
    12961 cggacgcccg gacgctgaca ccctgcaact ccagctcgac tacgtgatga gcgcctaccg
    13021 ggattcacgg atcggcgact ggctgtacgg gccgggcgcg aaggcgctca ctgacgccgg
    13081 gttcagccgc gtggtggccc agcccgagtc ggccgcgctg cgcacctacc tgctccgcgt
    13141 cggcttcacc gagacggacg cggggctggt gcgccaactg cgcccttgaa aaccctcggc
    13201 ggtagttgaa caataaatca tgactctctt cgggctgccg ctgcacccac tcgtcgtcca
    13261 cgccgccgtc gtcctgctgc cactggccgc cttcggagcc attgtgatcg cactctccgc
    13321 gcgcgttcgg cagcgtttcg gctggctggt cttcgcagga gccttcgtgg cgggcggctc
    13381 ggtggccgca gcccgcatca ccggagagac cttcgccggc ggcactaccg gcaccggcac
    13441 gctcgccacc catatgacct ggggcttgct cgcaccttgg cccgcgaccg cactggcggt
    13501 gagcgcgctg ctactgatgc tcacgaacaa ggcaacgagc cgtccgctca agatcaccgt
    13561 tggcgcgctc acgatcgttt ccgccgtagc cgcgcttgcc gtgatcgtcg tggttggcca
    13621 ctccggcgcc tcctcggtct ggggcgcgcc ggcctaaggg caagctcacc cacccttcga
    13681 caagctcagg cttcgacagg ctcagggagc gtcggcgcct cactcgcaga agctgagctc
    13741 gccggcctgc accatctcgg gcttgcccgc gccgatgaag cgcatctggt tcggcagggt
    13801 gaacatcttc gaaccgtcgc cgaaaccgat gtcctcgagc ttgcccttcg ggtacttcgc
    13861 cttcagttcc ttgaagctca gcgtcagcgg cacatcgtcg gccaactgca atggtgccgc
    13921 gaactcctcg gacagctgga agccccacgc cgccagcgtc cgcggcgacg acttgtgcgt
    13981 gtccgcagcc acgaactgca cccacagccc gccaaaggtg cgggtttcgg cccacgggct
    14041 ggagtcgtcc agttcgcaca ggattccctg cgaggtttcg tccggtgcgc ccagctggtc
    14101 gctcagcaac tcggccaccg gcgcctggtt cgaaccgaag ttgaaggtgc ccaggccgtc
    14161 cccgcgcagc accaggctcg cggctggagc ctgactaggg gaaacacttg gcgaagcgga
    14221 cgtctcggca accgtgggcg tggcgctcac cgtcgccgag ggctgggcag aactcggcgc
    14281 cgtttcggtc ggcgtggtcg caggagcgga cgggttcacg gtcgcgcatg ccgcaagcag
    14341 cagcactgcc ggcaacgcga gaaatcgacg aagactcatc gggcacctcc ggtgggtaac
    14401 gggcctcggt ctcaaggtac cccagtagcc cacctcgcca tcgctgccgc ggcccgtggg
    14461 cactagcctg agtcgggtca gcggcaacca gtagtgagga gcagagatgg ctcaggagaa
    14521 gaagcgaatc ggaattctca ccgccggcgg cgacagcccc gggttgaacg cggccatccg
    14581 cggcttcggc aaagctgcga tcggcaacgg ctgggagctg ctcggctacc gggacggcat
    14641 ccgcggcctg gtcgagaacc gctactccct actcgatccg aaggcgctct ccggcatcct
    14701 caccatcggc ggcaccgtgc tcggcaccag ccgggacaaa gtgcatcgca tggtggtgga
    14761 cggcgaggtg cgcgacatgc gcgaccaggt ggtcgcgaac tacgagaagg agggcctctc
    14821 cggcctcgtc ctgctcggcg gcggcgggac ggcgaagaac gcgatgcgcc tggcccaggc
    14881 cggcctgaac gtgatcacgc tgccgaagac gatcgacaac gacatcgccc gcaccgacac
    14941 cacctttggc ttcgcgaccg ccatggagat cgccacggac gcgatcgacc ggctgcactc
    15001 gaccgcgcac agccaccacc ggatcattct caccgagatc atgggccacc gcgccggctg
    15061 gctgaccctc ggtgctggga tcgccggcgg tgccgacatc atcctgttgc ccgagattcc
    15121 ctattcggtc gacagcgtcg ccgacacgat caagcagcgg ctgcagaagg gctccacctt
    15181 ctcggtggtc gcggtggccg aaggtgctcg cgaccggcac gacatggccg actacgaggc
    15241 cgcgcaactg ctggtgaagg cggccaagag ccctgagtcc gcgcgcgcag ccaaggccca
    15301 cctggccaat gtcgaggacg ctcagcgcga gcacaccttc aagctcgcgc gcgagttgga
    15361 ggcggcgacc gggctggagt ctcgggtgtc gatcctgggc tacgtgcagc gcggtgggac
    15421 gccgtgtgct gccgaccggc tgctcgcgtc ccggctgggg accgcggcgg cgcacgcggt
    15481 ttctaagggc caattcggcg tgatggtggc cgcccgcggc gaggacaccg agttggtgcc
    15541 gttggaggag gtggccggac aggtgaagct ggtgcccacc gatcacgagt ggatcgcgac
    15601 cgcgcgcaag ctgggcaccg ggctgggcga ctgaggtgca gcgtcgattc gcgcagatag
    15661 acgtcttcgg cgacggtggg atcaccggca atccggtggc cgtggtgctg gacgggtccg
    15721 gactgagcga cgccaggttg caggccttcg cgtcctggac caacctctcg gagaccacgt
    15781 tcctgtttcc accaaccgac ccggccgccg actatcgcgt ccgcatcttc accgcgagcg
    15841 ccgagttgcc cttcgccggg cacccgaccc tgggtagcgc acacgcctgg ctggaggcgg
    15901 gcggacggcc gcagcgtccg ggcgtggtga tgcaggaatg cggggtcggg ctggtcgggg
    15961 tgcggctgga caacgcgcgg ttggccttcg ctgcaccacc gcggcgacgc agtggtcccc
    16021 tggacgaagc cacgatcacg ctcgcggaga tggtgctcgg catcccgcgc gagcagtggg
    16081 tcgcgcacga gtggggtgac aacggtgcac cctggatgat ggtgcaactc gcggacgcgg
    16141 acgccgtccg cagcgtgcgg cccaatcact cgcacctggg cgcgacccag catctcgggg
    16201 tgatcggttt ggcgtccgac cagttcgcgt acgaggtgcg cggcttcgtc ggcgactggg
    16261 aggatccggt caccggcagc ctgaacgccg ccgcggcgca gtggctgcgc ggacgcggcc
    16321 tcgtcccgtc cgagtacgtg gctgcgcaag gctcacagct cagccgcagt ggcgaggtct
    16381 tcatcaacga cgacggcacc cgaatctggg taggcggccg cgtccgcact gtgatcaccg
    16441 gctccgtcga cctctagtcc tgaacgaaaa ggggacggtt cctttttcgt tcaagttatc
    16501 cacaggaccg tcaacttgaa cgagggtgga accgtccgtc agtcgcgggt cgaaggcgtg
    16561 ggacgccgac tgtgcggggc atggctcggc gactaggctg gctgggttag ttgaaacgag
    16621 gtatccatgc ccgcactgcg ctcccgcacc accacccacg gccgcaacat ggccggcgcc
    16681 cgcgctctgt ggcgcgccac cggcatggtc gaatccgact tcggcaagcc catcgtggcg
    16741 atcgccaact cgttcaccca gttcgtaccg gggcacgtcc acctcaagga catgggtcaa
    16801 ctggtcgcgg gcgcggtcgc cgaagccggc ggcgtcggac gcgagttcaa caccatcgcg
    16861 gtcgatgacg gcatcgccat gggccacggc ggcatgctct attcgctgcc gagtcgcgag
    16921 ctgatcgccg actcggtcga gtacatggtc aatgcgcact gcgcggacgc cctgatctgc
    16981 atctccaatt gcgacaagat cacccccggc atgc
//
LOCUS       DAOPRQ010000057        16742 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_2898_length_16742_cov_12.258525, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000057 DAOPRQ010000000
VERSION     DAOPRQ010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 16742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16742
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2898_length_16742_cov_12.258525"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(477..1256)
                     /locus_tag="PKJ61_08405"
     CDS             complement(477..1256)
                     /locus_tag="PKJ61_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007077441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOC13887.1"
                     /translation="MLLGTGAADGIPNPFCDCQTCADYRRRGELRTPTSVLIDGRLLI
                     DPGPEATRQVSRFGRDLIGLAAILAGHAHHDHLDPALLLHRSWVTDAPLTVAGPAPVV
                     AAARDWVHPESAIEFRTLCAGDTIELAGYLVTALSAHHEAFGEALCYLVSDGRSTLLY
                     ATDTGLLPEATRAALSGRSVDLVLLEETFGERADKGDQHLDLATFVETVGALRDLGVV
                     TSSTRVVAVHLGHDNPPLAELHDRLAAGGAEALPDGTLLQV"
     gene            1504..2394
                     /locus_tag="PKJ61_08410"
     CDS             1504..2394
                     /locus_tag="PKJ61_08410"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOC13888.1"
                     /translation="MPTELLPTQTRMSNVSLDVGDLDAMTAFYRDVLLLEPLSEGRGE
                     VALGRGGRELVRLRHRRDLPRGERRTAGLFHTALLHPDAGSLATVLASVARQAPQLYT
                     GSGDHLVSQAFYLDDPEGNGVELYVDRPRAEWQWQGTNVVMDTLWLDPNRFLAEHLDD
                     NALDLVETAPDRRPALPREQVVGHVHLKVGQTAAAKEFYVDALGFEVTAEMPGALFVS
                     AGGYHHHMAMNTWTSQGVGLRAPALGLGLVAIEVPTMDDVSAAADRLRAKGLRVRADQ
                     GAIEVDDPWVNLVRIAAKAG"
     gene            complement(2724..3578)
                     /locus_tag="PKJ61_08415"
     CDS             complement(2724..3578)
                     /locus_tag="PKJ61_08415"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015071563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HOC13889.1"
                     /translation="MNPVAQVVAVMGRGVVAPQTPLMLADDLGFTRGDGCFDAVRVVV
                     GETTRVDHLDRHLARFARSSAALDLVAPDAAAWRELIASALAEWAVPGEAVLKLVRTR
                     GAEHSDGGLTELLTITQAPDATAARQGVIASTLNRGFASDAFHAAPWLLGGVKSLSYA
                     VNVAAKREAARRGVDEVIFTSSDGYLLEAPTAGLLVAADDQLWTTPIAGTGVLESVTI
                     NVIMDSARADGVRTHHALFRPDDLADTDGAWLVSAIRGVLPVLEVDGRSLPHDSELTR
                     HLARTAGF"
     gene            3672..3968
                     /locus_tag="PKJ61_08420"
     CDS             3672..3968
                     /locus_tag="PKJ61_08420"
                     /inference="COORDINATES: protein motif:HMM:NF017691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excalibur calcium-binding domain-containing
                     protein"
                     /protein_id="HOC13890.1"
                     /translation="MHSRSRIALALATALIGSLASTAVPLASAEAATHPAWTKNCTQL
                     NKKYPHGVGKVGAKDQSSGRKVTNFKKSNSVYKTAMSHNRGLDRDGDGIACEKA"
     gene            4070..4714
                     /locus_tag="PKJ61_08425"
     CDS             4070..4714
                     /locus_tag="PKJ61_08425"
                     /inference="COORDINATES: protein motif:HMM:NF019151.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease family protein"
                     /protein_id="HOC13891.1"
                     /translation="MAPAPTTGSAASPTDGPNEPPNATAATEQLDALRLGPRISPASL
                     GYLRSAFGDGWVDVDGNGCNQRDDVLYRDAIPGTVRVVVQGSCDHDVVAGAWTDPYSG
                     KELRFANLKDQVEAQAIQVDHIVPLAEAWVSGATDWSDERRGVFANDLTELAAVDGPT
                     NASKGSGDPAAWRPRKQFQCDYARRWIAIKTTWDLSVDVSEQRALVEMLGFCAS"
     gene            complement(4783..6213)
                     /gene="glnA"
                     /locus_tag="PKJ61_08430"
     CDS             complement(4783..6213)
                     /gene="glnA"
                     /locus_tag="PKJ61_08430"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004811889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HOC13892.1"
                     /translation="MFQGADDLLAYVKDEGVETIDIRFCDLPGVMQHFTVPAKAFGPE
                     VFEDGLAFDGSSIRGFQKIHESDMALLPDPTTAFIDPFRRSKTLAVSFFVHDPLTKEP
                     YSRDPRNIARKAENYLASAGFGDKAFFAPEAEFYIFDSARFETNANSSYYYIDSEAGA
                     WNTGAENDSDGRPNRGYKVKYKGGYFPVAPVDHFSDLRDDMVRNLEAVGLEVERAHHE
                     VGTAGQAEINYRFNTMLAAADEVMKFKYIIKNTAWQAGKTVTFMPKPIFGDNGSGMHV
                     HQSLWQGDTPLFYDEAGYGGLSDIARWYIGGLLKHAPSLLAFTNPTVNSYHRLVPGFE
                     APTNLVYSARNRSACIRIPLTGSNPKAKRIEFRCPDPSSNPYLAFAACLLAGLDGVQN
                     KIEPLAPVDKDLYELPPEEHALVPTVPASLDAVLDKLEADHDYLLAGDVFTPDLIETW
                     IDMKRSDIEAIRLRPHPHEFEMYYDL"
     gene            6325..6759
                     /locus_tag="PKJ61_08435"
     CDS             6325..6759
                     /locus_tag="PKJ61_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015069934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HOC13893.1"
                     /translation="MSQTEADTDAEYPGQSIGLPEHGRGSLASWFARIAALILDWGAS
                     MIVAVTLFGVGVLRDGGWRAWMILAVFFVQTTVLTALTGSSFGQLLARIGIVRLDGQR
                     LGWPRAAARAAMICLVLPTVVIGAERRALNDLVLGTVVVNLR"
     gene            6916..8739
                     /locus_tag="PKJ61_08440"
     CDS             6916..8739
                     /locus_tag="PKJ61_08440"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter family substrate-binding
                     protein"
                     /protein_id="HOC13894.1"
                     /translation="MSTKKLLAAASGLIAGALLFSACTPPTPSANPTATQSAISEKTT
                     VSVMWNQPFYSGNGLSGYGNATANNNILYLTNDSVTFYDKELNLTKNTSFGDYEKTSD
                     DPLTVKQTIADTASWSDGTPVTAADFILAYGAQSGLFNNYEAKLDDEGDVAGKGKDGK
                     VFFNSSNAGWSLIKDFPTVESDNKSFTYTYSKPFADWEKNLFTPGLPAHIIGKRALGI
                     DDPTQAAQAVLDAFKNKDNSALSKIANSWNSDWNFAEMPKDAGLVVGTGPYTISEFKK
                     GSYLTLTKNANYKGAHVPSIESITVRYNEDPQAAVQALQNGEVQLISPQSTQDILKQL
                     EAVQGIKMETGIEGTFEHIDLIFANKGPFDPATYGGDAEKALKVRQAFLLTIPRQQII
                     DTIIKPLYADAAIRNSYTQVPGAPNYAAVSEANGMGTAYAAPDIEKAKSLLAEAGATS
                     PSVRILYGEGNARREQQFQLIKESAEQAGFKVVDGGDANWGAMLSNINKYDASLFGWQ
                     SVSTAVSENDANYRTGGQNNFGKYSSKVVDGILDQLQTEIDPAKQAELLGQMEKQLVD
                     DGFGLSIFQFPSVTAYSDKLTGVDPITIAPTIFWNFWEWKI"
     gene            8888..>9231
                     /locus_tag="PKJ61_08445"
     CDS             8888..>9231
                     /locus_tag="PKJ61_08445"
                     /inference="COORDINATES: protein motif:HMM:NF040248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOC13895.1"
                     /translation="MVRFLARRLALALGILLTLSAFMYFMLDLAMDPLWDLRTSTSPN
                     KAELIQNRINQLGLDLPWWERYWIWLRHFVSGDFGLAWTTQQRVNEMLPGAIATSVQL
                     VFAATMIAILLGV"
     assembly_gap    9232..9331
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9332..10093
                     /locus_tag="PKJ61_08450"
     CDS             <9332..10093
                     /locus_tag="PKJ61_08450"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOC13896.1"
                     /translation="SALTTAVVGVVAHYAVQWLFYYVPGSWTLMLILLLVAIGCGAGI
                     GWLFGGPDRAVSMRGGALVALMMAVLTFVDRVMQGWSLYFNSSVIKGRPIATIGSETP
                     GLGGDFWITQLDRFTHLLLPTIALVLISFASYTRYQRGSMLEVLNQEYIRTARAKGLT
                     ERVTIVRHALRNALLPLASVVPVDIISLIGGAVITETIFGWNGMGKMFVTSLRQSEPD
                     PVMAYVMITGALAMVANLVADFLYALLDPRIRVNA"
     gene            10090..11205
                     /locus_tag="PKJ61_08455"
     CDS             10090..11205
                     /locus_tag="PKJ61_08455"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOC13897.1"
                     /translation="MTTNPPLDPASDLSGPGRGDNAIEAKEVEGLSQGQIVWRRFLRH
                     RGAMFGLILLALVAIISFTAMGIGPIPGLWKYQDYNRSNPEILNGGAPTLSIPGFGSG
                     GFAIGEHPFGQDDIGRDNFALVMKGVQTSLLVMIVMSIVALVIGVTVGALAGYYRKRV
                     DQALMRFTDLVITLPVIVLGAVLGKLLSTIPIKLDWSQEVREGISASMPLLLAVMLGL
                     ILWPSLARLVRGEFLTLREREFVDSARVAGASDWRIITKHILPNAVGVIIVNTTLLMS
                     TAVVLETALSFLGFGINPPLISLGNLISTYQGAFSTRPWLFWWPGLFIILIALSVNFI
                     GDGLRDAFDPRTKRIPSEREMARTLIKANRAATGGAA"
     gene            11202..12995
                     /locus_tag="PKJ61_08460"
     CDS             11202..12995
                     /locus_tag="PKJ61_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOC13898.1"
                     /translation="MSKQNVVETNLEGAVRLSRRIGDGTVKKGDVILEVKDLGVKFWV
                     NGEWFDAAENVDYDLRAGEVLAIVGESGSGKTQSSMSLIGLLPPNGRASGSAKLKGVE
                     LIGLTGSRMREVRGNEIAVIFQEPMTALNPVYTVGFQIVETLRTHFVMAPAAAKARAI
                     ELMRLVEIPEPESSFDKYPHQLSGGQRQRIMIAQSLACDPKLLIADEPTTALDVTVQA
                     EILKLMRGLRERVDAAIILITHDMGVVADMADRIVVMKDGRVVEHGTSDEIFTAPKHP
                     YTQSLLAAVPHLGSQTGDDHIVVRESLVSDGKNPVPLADGDPDVTPALLMEGVSIDYP
                     KQGRRPVFRAAHDINLRIDPGRVLGLVGESGSGKTTIGRAAVALLPVADGRLQVAGRD
                     ITHASNADLYPFRRDVGIVFQDPGSSLNPRLPVGESIGEPLYLHEKLRGSELSKRVEA
                     LLDSVELPRAMRNRYPHELSGGQRQRIGIARSLALEPKLLIADEPTSALDVSVQARVL
                     QLFADLQERYGFACLFISHDLAVVEMLSSRIAVMKDGRLVEVGPTAEIVNSPQHPYTQ
                     RLLSAVPVPDPAEQKKRRELRDAIIEQQQLV"
     gene            complement(13135..13208)
                     /locus_tag="PKJ61_08465"
     tRNA            complement(13135..13208)
                     /locus_tag="PKJ61_08465"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13172..13174),aa:Phe,seq:gaa)
     gene            complement(13230..13303)
                     /locus_tag="PKJ61_08470"
     tRNA            complement(13230..13303)
                     /locus_tag="PKJ61_08470"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13267..13269),aa:Asp,seq:gtc)
     gene            complement(13399..13471)
                     /locus_tag="PKJ61_08475"
     tRNA            complement(13399..13471)
                     /locus_tag="PKJ61_08475"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13435..13437),aa:Glu,seq:ttc)
     gene            complement(13623..14990)
                     /locus_tag="PKJ61_08480"
     CDS             complement(13623..14990)
                     /locus_tag="PKJ61_08480"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HOC13899.1"
                     /translation="MNRALPSGRAALAWIVFAVTLVAPSLVATPASAALTTTYTAHTS
                     VNVRAKASTSSTVLGQLAEGQTVLAAGAITGDWLPIKYAGKTAYAWAEYLTVNPTPAT
                     EVTSGPAGKKTVTKKKAPVRAGASIEAAVTRTLAKKFVLRVTGLTSGDFTQVNVYGEL
                     QWVLTGHLSKATDTLPDVVARHKTTEPLALRAEPSATAKSRGTIKKSKTVGTTGVHSG
                     SFSQVVYAGKVGWVLTGYLKAAKGTPAELVLPRAAGKRYITLPDVVMFAGPDAAAAPT
                     ATFAFGTLVRITKQVQGDFTQVIWNGTTSWITSSSLSLAKPVPVAAATTPTTATPGTV
                     DLGSDSLNKLEPYAKVAVPEVRANFPQIKTIYGWRSSSSYSEDHPSGRAIDIMIPSYK
                     SNIALGDSIAQYFIDNGARLHVSYVIWQQRNYRLTRGYWVAMADRGGDTANHLDHVHV
                     SFDAP"
     gene            15198..15551
                     /locus_tag="PKJ61_08485"
     CDS             15198..15551
                     /locus_tag="PKJ61_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HOC13900.1"
                     /translation="MALRVTDQRFEELVDVALAQIPAELLDQVENCALIIEDEPDPEY
                     GDVLGFYDGTPLSERTSQYSGVLPDRILIFRGPLLRMVASEDELVDEVRITVWHELAH
                     YFGIDDDELDELGYA"
     gene            complement(15852..16580)
                     /locus_tag="PKJ61_08490"
     CDS             complement(15852..16580)
                     /locus_tag="PKJ61_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13901.1"
                     /translation="MLLIGIAVVVYGWLADRVDTQRRQEALTSAPDRAIPGLDASAAS
                     PEYRTEDELLRGADVRRPEVPAEAVARALRRLAGSPGMAFGHASDDFANAVAGIDELD
                     QPNLGSGTGFARLRQRSGGIERLCVLGAPRILVCDSEINSIRELIDFLAKAQADRAPV
                     VIVAPSVASDVLATLRVNSVQGHVACAVVLIPDATKRAQFASLVGAMPVGVDDLRAGY
                     LPSSALGTCGTWVSSRQQLWVVGE"
     gene            16669..16741
                     /locus_tag="PKJ61_08495"
     tRNA            16669..16741
                     /locus_tag="PKJ61_08495"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16702..16704,aa:Lys,seq:ttt)
BASE COUNT         2861 a         5279 c         5684 g         2818 t
ORIGIN      
        1 ctaggcttct cagctggtcg cgaaggagct caccgagctt ccgcacaccc agctcgcgga
       61 cgtcatcatt ccgggccggg tggccgagct cgccgccggc cgcgacgtca ccccgatctg
      121 gcgcaacggg atcggcgggg tgaccttcgc catcgacggg ggtgccgagt atgtgaagca
      181 cggccccgag cacccggagt cccagccgac cgccgagttc gcgcgactgc gctgggtgtc
      241 gcgtcaagtg cccgcacccg aaccgatcga ccacggcgtg gacgcctctg gctactgctg
      301 gctgcgcacc agaggaatcg caggcacgtc agccgtgctc aacggctggc gtgaccaccc
      361 cttggatttc gggacaaagc tgtcgccagt cgacgcctct ggcggacacc cccgcaattc
      421 cgttcactgc tggcaggttt gtcccattcg ttggatcggg gaggttgaag cattcgctag
      481 acctgcagca gtgtcccgtc cggcaacgcc tccgcgccgc cagcggccag ccggtcgtgg
      541 agttcggcca gtggcgggtt gtcgtggccc agatgcaccg ccaccactct ggtgctcgag
      601 gtcaccacgc cgaggtcgcg caaagcacct accgtctcca cgaaggtggc caggtcaagg
      661 tgttggtcgc ccttgtcggc gcgctcgccg aaggtctcct ccagcagtac gaggtcaacc
      721 gaccgaccac tgagggccgc gcgcgtggct tcgggtagca acccggtgtc ggtggcgtac
      781 agcagggtgc tgcgcccgtc cgagacgaga tagcacagcg cttcgccgaa ggcttcgtgg
      841 tgggcggaca gcgccgtcac caggtagccg gccaactcaa tcgtgtcgcc ggcgcacagc
      901 gtccggaact cgatcgcgga ctccgggtgc acccagtccc gggcggccgc gaccaccggc
      961 gccggtcctg cgaccgtcag gggcgcgtcg gttacccagg accggtgcaa cagcagcgcg
     1021 ggatcgagat ggtcgtggtg cgcgtgtccg gccaggatcg cggccagccc gatgaggtcg
     1081 cgaccgaagc gggacacctg ccgggtggct tccggtcccg ggtcgatcag cagccgtccg
     1141 tcgatcagga cggacgtcgg cgtgcgcaac tcgccacgtc gccggtagtc cgcgcaggtc
     1201 tggcagtcac agaatgggtt cgggatgccg tcggcggctc ccgtgccgag cagcatcact
     1261 tccaccaggt caggttaggg caaacgcgtc gcgaccccgc cggctagctc cggcgaggtc
     1321 gcaacacctg aaggggaaat cgggtcgggt tctgccatct gcctcctgtg gatcggactg
     1381 cttgctcatt gggttcagct aatcccagcg gcatgtgccc ccgatcagag tttcctgtgc
     1441 agacgccaag ggaatatcca ctggacagag ttggttgaat agtcaactaa ctaaggagaa
     1501 tgcgtgccca ccgaattgct gcccacccag acccgtatgt cgaacgtgag cctcgatgtc
     1561 ggcgacctcg acgccatgac cgcgttctat cgggacgtgc tgttgcttga gccgctcagt
     1621 gaaggccgtg gtgaggtcgc ccttggccgc ggcggacgcg aactcgtccg gttgcgacac
     1681 cgccgcgacc tgccgcgcgg tgagcggcgt accgcaggcc tgttccacac cgcactccta
     1741 cacccggacg ccgggtcgct cgccacagtg ctggcgtcag tggctcgtca ggcgcctcag
     1801 ctctacacgg gcagcggcga ccacttggtc tcgcaggcgt tttacctcga cgacccggag
     1861 ggcaacgggg ttgagttgta cgtcgatcgg ccccgtgccg agtggcagtg gcaggggacc
     1921 aatgtggtga tggataccct ttggctcgac ccgaaccgct tcctcgccga acacctggac
     1981 gacaacgccc tcgacctggt cgagacggcg cctgatcgcc gtccggcgtt gcctcgtgag
     2041 caggtcgttg gacacgtcca tctcaaggtc ggccaaaccg cggccgccaa ggagttctat
     2101 gtcgacgcgc tcggcttcga agtgaccgcg gagatgccgg gggcactgtt cgtgtcggcc
     2161 ggcgggtatc accatcacat ggcgatgaac acctggacga gtcagggggt ggggctgcgc
     2221 gcgcccgcgc tcggtcttgg cctggtcgcc atcgaggttc ccacgatgga cgacgtgagc
     2281 gctgccgcgg accgactccg cgcgaagggg ctgagggtcc gtgccgatca gggcgcgatc
     2341 gaagtggacg acccctgggt caacctagtg cgaatcgcag cgaaggctgg gtgaattcga
     2401 ccatcaggct ttgtcgcaag cggaaattca agctgttcgg cgggcgtcgg cgcgacgacc
     2461 cgcggaagag ctgccacggg ttccggaggc gtcccctccc aagcaccccc cacagaacct
     2521 gcggcagtcc agtcctccca cgaaaccgca cgattgacat cgggaggcgg ttccgagcga
     2581 ctaagtacag ggtactacag gaaactctac ggtagggggt attagggaga attcggcagg
     2641 aggtgaccaa tggccgatca tctgagcttg tcaatcggca tttccggtga cggtctgtgg
     2701 tcgcgaaccg aattgcctag gcctcagaat ccagcggtcc gggccagatg gcgggtgagt
     2761 tcggagtcgt gcggcagcga tcgcccgtcg acttcgagca ccggaagcac gcctcggatc
     2821 gccgacacca gccaggcgcc gtcggtatcg gccaagtcgt cgggccggaa cagggcgtgg
     2881 tgcgtgcgga cgccgtcagc gcgtgcggag tccatgatca cgttgatggt caccgactcg
     2941 agcacaccgg tgccggcgat cggggtcgtc cagagctggt cgtcggccgc gaccagcagg
     3001 cctgcggtgg gcgcttcgag aaggtaaccg tcactggagg tgaagatcac ttcatccact
     3061 ccgcggcggg ccgcctcacg cttggcggcg acgttcaccg cgtacgaaag actcttcacg
     3121 ccaccgagca gccacggcgc ggcgtggaag gcatcgctgg cgaagccgcg gttgagcgtg
     3181 ctggcgatga cgccctgccg ggccgcggtc gcgtccggcg cctgggtgat ggtgagcagt
     3241 tcggtgagcc caccgtcgga atgctcggct ccacgggtgc ggacgagctt cagcacggcc
     3301 tcgccgggca ccgcccactc ggccagggcg gacgcgatca gctcccgcca ggcggcggcg
     3361 tccggtgcca ccaggtcgag cgcggccgac gagcgggcga agcgggccag gtggcggtcc
     3421 aggtggtcga cccgggtggt ttcgccgacc accacccgga cggcgtcgaa acaaccgtcg
     3481 ccacgggtga agccgaggtc atcggcgagc atcaaagggg tttgcggggc gacgacgccg
     3541 cggcccatga ccgcgacgac ctgggctacc gggttcacgc tgcatacctt aggccaacgg
     3601 gtgcctgctg acggaggcgc cgacttctca aagccccggc gtccgttcat gcgctctgga
     3661 agactgctgg tatgcactcc cgcagcagga tcgccctcgc attggccacc gctctcatcg
     3721 gttcgctggc cagcaccgcc gtgccgctgg cctcggccga agcggccacc catccggcgt
     3781 ggaccaagaa ctgcacgcag ctgaataaga agtacccgca cggagtcggc aaggtgggcg
     3841 cgaaggacca atcctctggc aggaaagtga ccaacttcaa gaagtccaac agcgtgtaca
     3901 agacggcgat gtcacacaac cggggtctag accgcgacgg cgatggcatc gcctgcgaga
     3961 aggcgtgaac tcaagcgttg cgctagcgac gttggtcgca attggtgcgg tgctttgcct
     4021 gaccgcctgc acaacggacg ggcgagcgtc ggttgatcac ccgaacgcga tggcaccagc
     4081 gccgacgacc ggatcagcgg ccagcccgac ggacgggccg aacgaaccgc cgaacgccac
     4141 cgctgcgacc gagcagttgg acgcgttgcg gttaggccca cggatctcgc cggcgagcct
     4201 gggctacctg cgtagtgcgt tcggcgacgg ctgggtcgac gttgacggca acggctgcaa
     4261 ccagcgtgac gacgtgctgt atcgggacgc gattccgggc acggttcgag tggtcgttca
     4321 gggcagctgc gaccacgatg tcgtggcggg ggcgtggacg gatccgtact cgggcaagga
     4381 actcaggttc gccaacctca aggaccaggt agaagcccag gccatccagg tcgaccacat
     4441 cgtgccgctt gccgaggcct gggtgtccgg tgcaacggac tggtcggacg agcggcgggg
     4501 ggtcttcgcc aacgacctca cggaactggc agccgtcgac gggccgacca atgcgagcaa
     4561 gggcagtggc gacccggccg cgtggcggcc caggaagcaa ttccagtgcg actacgcccg
     4621 acgctggatc gcgatcaaga ccacgtggga cctctcggtc gacgtcagcg agcagcgggc
     4681 gctcgtcgag atgctcggct tctgcgccag ctgatcgcac gcgcagaact gccccggggc
     4741 cgaagccccg gggcagtcga gcagacctac gctttttcga gctcagaggt cgtagtacat
     4801 ctcgaactcg tgaggatgcg gacgcagccg gatcgcctcg atgtcgctgc gcttcatgtc
     4861 gatccaggtc tcgatcaggt ccggggtgaa cacgtcgccg gcgagcaggt agtcgtgatc
     4921 ggcttccagc ttgtcgagca ccgcgtccag cgaagccggg acggtcggca ccagcgcgtg
     4981 ctcctcgggc ggcagctcgt agaggtcctt gtccaccgga gccagcggct cgatcttgtt
     5041 ctgcacaccg tccaggccgg ccagcaggca cgcggcgaac gccaggtacg ggttgctgga
     5101 cgggtcgggg cagcggaact cgatgcgctt ggccttcggg ttcgagccgg tgagcggaat
     5161 ccggatgcag gccgagcggt tgcgagccga gtagaccaag ttggtcgggg cctcgaagcc
     5221 gggcaccagg cggtggtagg agttcaccgt cgggttggtg aacgccagca gcgacggcgc
     5281 gtgcttgagc agaccgccga tgtaccagcg ggcgatgtcg gaaaggccgc cgtagccggc
     5341 ctcgtcgtag aacaggggag tgtcgccctg ccacagcgac tggtgcacgt gcatgcccga
     5401 gccgttgtca ccgaagatcg gcttcggcat gaaggtgacg gtcttgccgg cctgccaggc
     5461 ggtgttcttg atgatgtact tgaacttcat cacctcgtcg gccgcggcca gcatggtgtt
     5521 gaagcggtag ttgatctcgg cctggccggc ggtgccgacc tcgtggtgag cgcgctcgac
     5581 ctcaaggccc acggcctcca ggttgcggac catgtcgtcg cgcaggtcgg agaagtggtc
     5641 caccggcgcc accgggaagt agccgccctt gtacttgacc ttgtagccgc ggttcggccg
     5701 gccgtccgag tcgttctccg cgccggtgtt ccaggcgccg gcctcggaat cgatgtagta
     5761 gtaggacgag ttcgcgttcg tctcgaagcg agcggagtcg aagatgtaga actcggcctc
     5821 gggcgcgaag aacgccttgt cgccgaagcc tgcgctggcc aggtagttct cagccttgcg
     5881 ggcaatgttc cgcgggtcgc ggctgtacgg ctccttggtc agcgggtcgt gcacgaagaa
     5941 gctcacggcc agagtcttcg accggcggaa cgggtcgatg aaagcggtgg tcgggtcggg
     6001 cagcagggcc atgtcggatt catggatctt ctggaagccg cggatcgacg agccgtcgaa
     6061 ggccaggccg tcctcgaaga cctcggggcc gaaggccttg gcaggcacgg tgaagtgctg
     6121 catcacgccc ggaagatcgc agaagcggat gtcgatggtc tcgacgccct catccttgac
     6181 gtaggccaac aggtcgtcgg cgccttggaa catctttcct ccacgaagta aggggttgaa
     6241 ctctgcaaac cttagggacg cgccgtaacc gccgagttgc acctttgttt cagccgggta
     6301 acaacgcctg gataggctgg cgccgtgagc cagaccgaag ccgacaccga cgccgagtac
     6361 cccggccaga gcatcggcct gcccgagcac ggacgcggct cgctggcgtc ctggttcgcg
     6421 cggatcgcgg ccctgatcct cgactggggc gcgtccatga tcgtcgcggt gaccctcttc
     6481 ggcgtcggcg tgctgcgcga cgggggctgg cgggcatgga tgatcctggc ggtgttcttc
     6541 gtccagacca ccgtgctcac tgctcttacc ggcagcagct tcggtcagct gctcgcgcgc
     6601 atcggcatcg tccggctgga cgggcagcgc ctgggctggc cgcgggcggc ggcgcgggcg
     6661 gcgatgatct gcctggtact gcccaccgtc gtgatcgggg ccgagcgacg cgcgctcaat
     6721 gacctcgtct tggggacagt tgttgtgaat ctgcgctgaa tgctgagatt tcgcttcgat
     6781 aacaactcca tacagctatc ttccgcgctt tgtaatggcc ggggtgcccg agtactttgg
     6841 acgaagctca ccaagcggat ctgcgctcat gcgtgcatgg gcgcagatcc gtttcgatca
     6901 ctggagggaa agaaattgag caccaagaag ctccttgctg cggcatcggg cctgatcgcg
     6961 ggcgcgctgc tgttcagcgc ttgcaccccg cccactccgt cggcgaaccc caccgccacc
     7021 cagtcggcca tcagcgagaa gaccaccgtc tcggtgatgt ggaaccagcc gttctattcg
     7081 ggtaacggcc tcagcgggta cggcaacgcc acagccaaca acaacatctt gtacctgacg
     7141 aacgactcgg tcacgttcta cgacaaggaa ctgaacctga ccaagaacac cagcttcggt
     7201 gactacgaga agacctcgga cgacccgctg accgtcaagc agaccatcgc ggacaccgcc
     7261 agctggtcgg acggcacccc ggtcaccgct gccgacttca tcctcgccta cggcgcccag
     7321 agcggcctct tcaacaacta cgaggccaag ctggacgacg agggcgacgt cgccggcaag
     7381 ggcaaggacg gcaaggtctt cttcaactcc agcaatgcgg gctggtccct gatcaaggac
     7441 ttcccgaccg tcgagtcgga caacaagtcc ttcacctaca cctactccaa gccgttcgcc
     7501 gactgggaga agaacctgtt caccccgggt ctgccggcgc acatcatcgg caagcgcgcg
     7561 ttgggcattg acgatcccac ccaggccgct caggccgtcc tggacgcgtt caagaacaag
     7621 gacaactccg cgctgtccaa gatcgccaac tcctggaact ccgactggaa cttcgccgag
     7681 atgccgaagg acgccggtct ggtcgtcggc accggtccgt acaccatctc cgagttcaag
     7741 aagggcagct acctgaccct gaccaagaac gcgaactaca agggcgccca cgtcccgagc
     7801 atcgagtcca tcaccgtccg ctacaacgag gatccgcagg ctgcggtaca ggcgttgcag
     7861 aacggtgagg tgcagctgat ctcgccgcag tcgacccagg acatcctcaa gcagctcgag
     7921 gccgtgcagg gcatcaagat ggagaccggc atcgagggta ccttcgagca catcgacctg
     7981 atcttcgcca acaagggccc gttcgacccg gcgacctacg gtggcgacgc tgagaaggcg
     8041 ctcaaggtgc ggcaggcgtt cctgctgacc atcccgcgtc agcagatcat cgacaccatc
     8101 atcaagccgc tctacgccga cgctgcgatc cgcaactcgt acacccaggt gccgggcgcg
     8161 ccgaactatg ccgctgtttc tgaggccaac ggcatgggca ccgcctacgc cgcgccggat
     8221 attgagaagg cgaagtcgct gctggccgag gccggcgcga ccagccccag cgtccgcatt
     8281 ctctacggtg agggcaacgc ccgccgtgag cagcagttcc agctgatcaa ggaatccgcc
     8341 gagcaggccg gcttcaaggt cgtggacggc ggcgacgcca actggggcgc catgctgagc
     8401 aacatcaaca agtacgacgc ctcgctgttc ggctggcagt cggtctccac cgcggtgtcg
     8461 gagaacgatg cgaactaccg caccggtggc cagaacaact tcggcaagta ctccagcaag
     8521 gttgtggacg gcatcctgga tcagctgcag accgagatcg acccggccaa gcaggccgag
     8581 ctcctcggcc agatggagaa gcagctggtg gatgacggct tcggcctgtc catcttccag
     8641 ttcccgtcgg tgaccgccta cagcgacaag ctgaccgggg tcgacccgat caccatcgcg
     8701 ccgacgatct tctggaactt ctgggagtgg aagatctgaa gtcctgagta ggcggtgcgg
     8761 cgctcactga gggtcgcgcc agctcggaac ggggcgtcag ggatgaaccc ctggcgcccc
     8821 gttcgcatga ctactcccgg cttgccggta gtcttcctgt cctactgtta acctccgaga
     8881 gttcaacatg gttcgttttc tggcgcgacg actcgccttg gcgttgggca tcctgctgac
     8941 gctgtctgcg ttcatgtatt tcatgctcga cctggcgatg gacccgctct gggacctgcg
     9001 gacgagcaca tctccgaaca aggcggagtt gatccagaac cggatcaacc agctgggtct
     9061 ggacctgccc tggtgggagc gttactggat ctggctacga catttcgtgt ccggcgactt
     9121 cggtctggct tggacgaccc agcagcgggt gaacgagatg ctgcccggcg cgatcgcgac
     9181 ctcggtgcag ctggtgttcg ccgccaccat gatcgcgatc ctgctcggtg tnnnnnnnnn
     9241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntcggcgctc accaccgcgg tggtgggcgt
     9361 ggtcgcccac tacgcggtgc aatggctctt ctactacgta ccgggcagct ggacgctgat
     9421 gctgatcctg ctgctggtcg ccatcggctg cggcgccggc atcggctggc tcttcggcgg
     9481 acccgaccga gcggtgtcga tgcgcggcgg tgccctggtc gccctgatga tggccgtgct
     9541 caccttcgtc gaccgggtga tgcagggctg gtcgctctac ttcaactcga gtgtgatcaa
     9601 gggacgcccg atcgcgacga tcggctcgga gacgcccggt ctgggcggtg acttctggat
     9661 cacccagctc gaccgattca cccacctgct gctgcccacc atcgcgctgg tgctgatctc
     9721 gttcgcgtcc tacacccgct accagcgtgg atcgatgctg gaagtgctca atcaggagta
     9781 catccggacg gcgcgcgcca agggtctgac cgaacgggtg acgatcgtgc ggcacgcgtt
     9841 gcgaaatgcg ctgctgccgc tcgcgtccgt cgtgccggtg gacatcatct ccctgatcgg
     9901 cggtgcggtg atcaccgaga ccatcttcgg ctggaacggc atgggcaaga tgttcgtgac
     9961 ctcgctgcgc cagagcgagc cggatccggt gatggcctac gtgatgatca ccggcgcact
    10021 cgccatggtg gcgaacctgg tggccgactt cctttacgcc cttctcgacc cacggatccg
    10081 ggtgaacgca tgacgacgaa tcctccgctc gaccccgcgt ccgacctctc cggccccgga
    10141 cgcggcgaca acgccatcga agccaaagag gtcgaaggcc tctcccaggg acagatcgtc
    10201 tggcggcggt tcctgcgcca ccgcggcgcg atgttcgggc tgatcctgct cgcgctggtc
    10261 gcgatcattt cgttcactgc catgggaatc ggcccgatcc ccggcctgtg gaagtaccag
    10321 gactacaacc gcagcaaccc ggagatcctc aacggtggtg cgccgacttt gagcattccg
    10381 ggcttcggca gcggtggctt cgcgatcggc gagcacccgt tcggccagga cgacatcggt
    10441 cgggacaact tcgcgctggt gatgaagggc gtgcagacgt ccctgctggt gatgatcgtg
    10501 atgagcatcg tggcgttggt gatcggcgtc accgtcggcg cgctcgctgg ctactaccgc
    10561 aagcgcgtcg accaggccct gatgcggttc accgacctgg tgatcacgct gccggtgatc
    10621 gtgctcggcg ccgtgctggg caagctgctg tcgaccatcc cgatcaagct cgattggagc
    10681 caggaagtac gtgagggaat cagtgcaagc atgccgctgc tgctggcggt gatgctgggg
    10741 ctgatcctgt ggccgagcct ggcccggctg gtgcgtgggg agttcctcac ccttcgggaa
    10801 cgcgagttcg tggattcggc gcgggtcgcc ggcgcaagcg actggcggat catcaccaag
    10861 cacatcctgc cgaacgcggt tggcgtgatc atcgtcaaca ccacgttgct gatgagcacc
    10921 gcggtggtgc tggagacggc gctgtcgttc ctcggtttcg gcatcaaccc gccgttgatt
    10981 tcgctgggca acctgatctc gacctatcag ggcgcgttct ctacgcggcc atggctgttc
    11041 tggtggccgg gcctgttcat catcctgatc gcgctgagcg tcaacttcat cggggacggg
    11101 ctgcgggacg cgttcgatcc gcggacgaaa cggatcccgt ccgagcggga gatggcgcgg
    11161 acgctgatca aggccaaccg tgcggcgacc gggggtgcgg cgtgagcaag cagaacgtgg
    11221 tggagaccaa ccttgagggc gccgtccggc tgtcccggcg gatcggcgac ggcacggtca
    11281 agaagggcga cgtgatcctt gaggtcaagg acctcggggt gaagttctgg gtgaacggcg
    11341 agtggttcga cgccgccgag aacgtcgact acgacctgcg cgcaggcgag gtgctggcga
    11401 tcgttggcga gtccggttcg ggcaagaccc agtcctcgat gagcctgatc ggactgctgc
    11461 cgcccaacgg gcgcgcgtcc ggatcggcga agctgaaggg cgtcgagctg atcgggttga
    11521 ccggcagccg gatgcgagag gtgcgtggca acgagatcgc ggtgatcttt caggagccga
    11581 tgactgcgtt gaacccggtg tacacggtcg gcttccagat cgtggagacg ctgcggacgc
    11641 acttcgtgat ggccccggcg gcggcgaagg cgcgggcgat cgagctgatg cggctggtgg
    11701 agattcccga gccggagtcg agcttcgaca agtacccgca ccagctttcc ggggggcagc
    11761 ggcagcggat catgatcgcg cagtcgctgg cctgtgatcc caagttgctg atcgcggacg
    11821 agcccaccac cgctctggac gtgaccgtgc aggccgagat cctgaagctg atgcgcgggc
    11881 tgcgtgagcg ggtggacgcg gcgatcatcc tgatcaccca cgacatgggc gtggtggccg
    11941 acatggccga ccggatcgta gtgatgaagg acggccgggt cgtcgagcac ggcacttcgg
    12001 acgagatctt caccgctccc aagcatccgt acacccagtc gctgctggct gcggttccgc
    12061 acctcggttc gcagacgggc gacgaccaca tcgtggtgcg cgagtcgttg gtttcggacg
    12121 ggaagaaccc ggtgccgctt gcggacggtg atcccgacgt gaccccggcg ctgctgatgg
    12181 agggtgtcag catcgactac ccgaagcagg ggcggcgtcc ggtgttccgg gcagcgcacg
    12241 acatcaacct gcggatcgat ccgggccgcg tgctcggctt ggtgggggag tcgggctcag
    12301 gcaagacgac gatcggacgc gcagccgtgg cgctgctgcc ggtggccgat ggacggctgc
    12361 aggttgccgg acgcgacatc acgcacgcct cgaacgcaga cctctacccg ttccggcggg
    12421 atgtcggcat cgtcttccag gatcccggat cgtcgctgaa cccgcggttg ccggtggggg
    12481 agtcgatcgg tgagccgctg tacctgcacg aaaagctgcg cggttcggag ctgtcgaagc
    12541 gggtggaggc gctgctcgat tcggtggagt tgccacgggc gatgcgtaac cgctacccgc
    12601 acgaattgtc gggcgggcag cggcagcgga tcggcatcgc gcgctcgctg gcgctggagc
    12661 cgaagctgct gatcgcggac gagcccacct cggcgctgga cgtgtcggtg caggcccgcg
    12721 tgctgcagct cttcgccgac ctgcaggagc gctacggctt cgcctgcctg ttcatcagcc
    12781 acgacctggc ggtggtggag atgctgagca gccggatcgc ggtgatgaag gacggccgcc
    12841 tggtggaggt cggccccacc gccgagatcg tgaactcgcc gcagcatccc tacacccagc
    12901 gcctgctctc cgcagtgccc gtgcccgacc ccgctgagca gaagaagcgc cgcgagctcc
    12961 gggacgcgat catcgagcaa cagcagctgg tttagtagcc cacgtagctg aaccctgagc
    13021 cggcgtgcgg ctcggggttc ggctgcttct tgagcttcgt cgagagcttg cgccgcgttg
    13081 gcacatcgcc ctgacaagca aaaatgcgac gaggggtttc ctcgtcgcac gcggtggcca
    13141 ggaccgggat cgaaccggtg acctttcact tttcaggcga acgctactac catctgagct
    13201 acctggccga gaagcaatag ccgcttcagc gaccccgacg ggactcgaac ccgcgacctc
    13261 cgccgtgaca gggcggcgcg ctaaccaact gcgctacggg gccttggaag gccttgccag
    13321 ccgttcccgg ccagccccga aactataacg taacccatcg ctcgcacgaa aatcggcccg
    13381 ctgtctgctc cggcttcgca tccccaacgg gattcgaacc cgcgctgccg ccttgaaagg
    13441 gcggtgtcct aggccgctag acgatgggga cctgaccgtt gaactatacg ggtacggcgt
    13501 tgggccccac aaaaccgccc caccggcgac gcgcgctgag cctggcggag tacgcggctg
    13561 gtcgagcttg cccgagaccc cgacgaccgc agcgcgcggc tgggccctcg cgacacgagt
    13621 cactacggcg cgtcgaacga cacgtgcacg tggtccaggt gattggcggt gtccccgcca
    13681 cgatcagcca tggcgaccca gtagccgcga gtaaggcggt agttgcgctg ctgccagatg
    13741 acgtagctga cgtgcagccg ggcgccgttg tcgatgaagt actgggcgat gctgtccccg
    13801 agcgcgatgt tggacttgta gctcgggatc atgatgtcga tcgcgcggcc gctggggtga
    13861 tcttcggagt agctgcttga ggatcgccag ccgtagatcg tcttgatctg cgggaagttg
    13921 gcgcgcacct cgggcacggc gaccttggcg tagggctcga gcttgttcag gctgtcagag
    13981 ccgaggtcta ccgtgcctgg ggtggccgtg gttggagtcg tggcggccgc cacaggcaca
    14041 ggcttggcga ggctgaggga ggaggaggtg atccagctgg tggtgccgtt ccagatgact
    14101 tgagtgaagt cgccctgaac ctgcttggtg atgcgcacga gggtgccgaa ggcgaaggtg
    14161 gcggtgggtg cggcggccgc gtccgggccg gcgaacataa ctacgtccgg tagcgtgatg
    14221 tagcgcttcc cggctgcacg cgggaggacg agttcggcgg gtgtgccctt ggccgccttc
    14281 aggtagccgg tgagcaccca accgaccttg ccggcataga cgacctggct gaagctgccg
    14341 ctgtgaacgc ccgtggtgcc gacggtcttg gacttcttga tggtgccgcg gctcttggcg
    14401 gtggcggacg gttctgcccg cagggccagc ggctcggtgg tcttgtggcg ggcgaccacg
    14461 tccggaagcg tgtcggtggc cttcgagagg tgaccggtga gcacccactg cagctcgccg
    14521 tacacgttga cctgggtgaa gtcgccggag gtgaggccgg ttacgcgcag tacgaacttc
    14581 ttggcgaggg tgcgggtgac ggcggcctcg atgctggcgc cagcgcggac gggggccttc
    14641 ttcttggtga cggtcttctt gccggccggc ccactggtga cctcggtggc tggggtgggg
    14701 ttgaccgtga ggtactcggc ccaggcgtag gcggtcttgc cggcgtactt gatgggtagc
    14761 cagtcgcccg tgatagcgcc ggcggcgagg acagtctggc cttcggcgag ttggccgagg
    14821 acggtcgagg aggtgctggc cttggcgcgg acgttgacgg aggtgtgggc ggtgtaggtg
    14881 gtggtcaagg cagcgctggc cggcgtggcg acgagcgacg gcgccaccag ggtgacggcg
    14941 aagacgatcc aggccagggc ggcccgacca cttgggagcg cacggttcac gacgaccgcc
    15001 ttcttcgggg gtgcctgctg gggctttgag gtgggacagg tgttcctcgg tggcgtggcc
    15061 ccacccgtcc tggtgccctc agctgtggac tgtagtaagc gtgcttgggt ggcgtcactg
    15121 cttgggtacg aacttgcggg gtcgggagag ggtgcgcggc cgcccggcgg agtgaacagc
    15181 gtggataggc tggccgcatg gcgctgcggg tgacggacca gcggttcgag gaactggtgg
    15241 atgtcgcgtt ggcgcagatc ccggccgaac tgctcgatca ggtggagaac tgcgccctga
    15301 tcatcgagga tgagcccgac cctgagtacg gtgacgtgct cggcttctat gacggaactc
    15361 ccttgtcaga gcgcacttcg cagtattcgg gagtgcttcc ggaccggatc ttgatcttcc
    15421 gcggcccgct gctgcgaatg gtggcgtccg aggatgagct ggtggacgag gtccgcatca
    15481 cggtgtggca cgaactggcc cactacttcg gaatcgacga tgacgaactg gacgagctgg
    15541 gctacgccta agccgcaacg cccgagaaaa aactcaccta cagccaaggt cgagagatcc
    15601 gcacggaacg cccaacgtta tcccggcgaa ggccgggatc tccacgcgag gtctgcgagc
    15661 ctagctggga tgcgttgcga gggcagacct cttcccttcc ttgacaacgc acggggagct
    15721 tcgcaggtga gcagctgttc cctcccaacg ttgcctgagg agcgtcgcga ggaacgagcg
    15781 aagcgtcacg aaggcccgcc acctgcaacc gatgccgccg accggggacg cctgagcttg
    15841 acgaggctcg atcactcacc aacgacccac agttgctgcc ggctactgac ccaggttccg
    15901 caggttccaa gcgccgacga cggcagatac ccggcccgaa gatcgtccac acccactggc
    15961 atagcgccga cgagcgaggc gaactgtgcc cgcttggtcg cgtccggaat gaggacgaca
    16021 gcgcacgcga cgtgcccctg cacgctgttg acgcgcaagg tcgcaagcac gtcggacgcg
    16081 acgctgggcg cgacgatgac gaccggcgcc cgatcggcct gagccttggc cagaaagtcg
    16141 atcagctccc gaatggaatt gatctcggag tcgcagacga gaatccgcgg tgccccaagc
    16201 acacaaagcc gctcgatgcc gccagaacgt tggcggagcc tggcgaagcc cgttcccgaa
    16261 ccaaggttcg gctgatcgag ctcgtcgata cccgcgacgg cattggcgaa gtcgtccgag
    16321 gcatgcccaa acgccatacc cggcgaccca gccagtcgtc gaagcgcgcg agccacagcc
    16381 tctgcgggaa cctccggacg ccgaacgtcg gccccacgaa gcagctcgtc ctcggtgcga
    16441 tattccggcg aggcggctga cgcatcgagg cccggaatcg cgcggtcagg cgccgaggtg
    16501 agggcttctt ggcgccgctg ggtatccacc cggtcagcca gccacccgta gacgacgaca
    16561 gcgatgccga tcagcagcac cagcccgatg ccgaggtagc cgaagttcac gcctccagtg
    16621 tgcatggccg cccgcgccgc aggtacgcta cgagggcgcg ccagcgtggg gcctatagct
    16681 caataggcag agcagcggac ttttaatccg cgggttctgg gttcgattcc cagtgggccc
    16741 ac
//
LOCUS       DAOPRQ010000058        16225 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_3024_length_16225_cov_13.375387, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000058 DAOPRQ010000000
VERSION     DAOPRQ010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 16225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16225
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3024_length_16225_cov_13.375387"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1406
                     /locus_tag="PKJ61_08500"
     CDS             <1..1406
                     /locus_tag="PKJ61_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016456770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5915 domain-containing protein"
                     /protein_id="HOC13902.1"
                     /translation="SSYKNVLCLGHILAEDGRKMSKHLGNILEPIPLMDTHGADAVRW
                     FMAAGGSPWASRRVGHGTIQETVRKVLMTFLNTVAFQSLYARANDWSPASTAAPGETE
                     HVLDRWLVSTRNTLTKQVTEALEDFDTQRAGSLLAAFVDDLSNWYVRRSRRRFWDGQP
                     GALQTLHDTLNVVTRLLAPLVPFITERVWQDLFVTTDSAAPASVHLASWPEADAAAID
                     AKLDEAMAVTRRIVELGRSARAEAKVKTRQPLRRALIPSGAFNLLDAELRAEVMAELN
                     VERVESFASAGDLVDYSAKGNFRNLGKRFGKDTPLVASAIASADAATLSADLATHGRA
                     EVLVADLGAVEVTPEDVIVSERPREGWSVVNEQGETVALDLELTPELVRAGLAREVIR
                     GVQEARKSSGFEVADRIRLAWQADDEATADAVRAHAEFIGAEVLAVEVAEAEPADDWF
                     ADAELGLRFAVTRVEQS"
     gene            1406..2134
                     /locus_tag="PKJ61_08505"
     CDS             1406..2134
                     /locus_tag="PKJ61_08505"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOC13903.1"
                     /translation="MSGPGGSESRLTPVVLLHAAGLTPQMWQAQVEAIAAQAEPAQRP
                     TQGAEVGAAQPAERQVMAPWIAGLRPGRPGELSLTGAAAQLVGTLDSYGIEQAILVGH
                     QLGAMVALQVAATEPERVAGLVLSGAMAAPGRVALAMQKGVIRMLPNRTLADSGASKA
                     DLIKALDVIASADFGKLEQVNTRALLIAGANDPLLAATRQLASRLPQARLVELSGAGP
                     HPNVEQPDAYNRLLADFLAWTPPA"
     gene            2116..>2413
                     /locus_tag="PKJ61_08510"
     CDS             2116..>2413
                     /locus_tag="PKJ61_08510"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012921613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOC13904.1"
                     /translation="MDAAGLTQRLVKRTRGRPGALALITADGITTVRHGCGPEADFEI
                     GSISKGVTGLLYHCALERGEVTADTQLGQLLPLADSPAAAIRLADLATHRSGLPR"
     assembly_gap    2414..2513
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2514..3145
                     /locus_tag="PKJ61_08515"
     CDS             <2514..3145
                     /locus_tag="PKJ61_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019510848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13905.1"
                     /translation="TATDLGPWIGWATPHPALGRSQPRPGRWHPGVRRSDGEAGAGAA
                     DAHDFYNAVLKKGKTDPAQEVKVARITAVVIGVLAIVGGIAANGQNIAFLVSLAFAVA
                     ACANLPSILMTLYWKRFTTRGSVWSIYTGLLGSILLIVFSPAISSPTWTNPVAWFPLT
                     SPTIVALPLAFLAGIIGTLTSRSPADPELQAEMEVRSLTGAGVAEVVAH"
     gene            3229..4158
                     /gene="rarD"
                     /locus_tag="PKJ61_08520"
     CDS             3229..4158
                     /gene="rarD"
                     /locus_tag="PKJ61_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006548736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HOC13906.1"
                     /translation="MPDANQAKLDSRGLLLAFLAYLLWGAFPLYFHLLSAADPVEIIG
                     HRLVWTNVFCLAGVLVWREWTHLRAVVADRKLFWRLTLAGVLVSANWLIYVWGVLNNH
                     IVDAALGYFINPLFTVALAVFVLGEKLRRAQLVAVAIGILAVVVIAVGYGQIPWVALS
                     LAVTFGIYGLVKKQVGGRVSPLVGLTVETTMLAPVALAYLVWLQASGHGAYLAHGPGL
                     TVALTLAGAVTAIPLLMFAAASSRIPLSMMGFIQYLTPVIQFALGVWVNHEEMPLARW
                     LGFGLVWLAVVVLTVDGVRAARRPDIIEPEVVN"
     gene            complement(4384..4719)
                     /locus_tag="PKJ61_08525"
     CDS             complement(4384..4719)
                     /locus_tag="PKJ61_08525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13907.1"
                     /translation="MSQTTPDESQGSWQQPPFAATNDPNQAPVVSGHVVVPGEAPPAP
                     SVLESTLNVVNGVIWPIAIFLGILGIGSFIGNIFMAVIASIVLGQITKELRRRRRPAE
                     LGGPPSDLR"
     gene            4880..6145
                     /locus_tag="PKJ61_08530"
     CDS             4880..6145
                     /locus_tag="PKJ61_08530"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002514581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOC13908.1"
                     /translation="MRVVQKFGGSSVADAESIKRVARRIVATQSAGNEVVVVISAMGD
                     TTDDLMDLAQQVSPNPRPRELDMLLTAGERMSAALLAMAINDLGADARSFTGSQAGVI
                     TTGTHGDARIIDITPGRIVSALEDGDIVIVAGFQGVSQTTKDVTTLGRGASDTTAVAL
                     AAALDAAYCEIYSDVDGIFTADPRIVPGARQIPQIGYEDMLEMAANGAKILHLRCVEY
                     ARREHVPVHVRSSFSDKPGTWVKDINYEESGMEQPLIEGIAHDRSEAKITIIGVPDRV
                     GEAAEIFTVVAEADVNIDMIVQNVSRVQEQATDISFTLAQQDGDKAIAALRAVQERIG
                     FLDVLYDDQVGKVSVVGVGMRSHPGVTARFFRALAEANVNIGMISTSEIRISVVVAID
                     DVDRAVRAGHTAFGLDSDEHAVVYGGTGR"
     gene            6291..6584
                     /locus_tag="PKJ61_08535"
     CDS             6291..6584
                     /locus_tag="PKJ61_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HOC13909.1"
                     /translation="MIFKRVGDSRPYPEHGYVPKQWAAIAPQQVRLDQLVTTKRTLDL
                     DALLEEDSTFYGDLFAHVVAFQGELYLEDGLHRALRAALQQRQTVHARVLELK"
     gene            6624..7148
                     /locus_tag="PKJ61_08540"
     CDS             6624..7148
                     /locus_tag="PKJ61_08540"
                     /inference="COORDINATES: protein motif:HMM:NF024791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HOC13910.1"
                     /translation="MRQFIRAIKTPVTLIVLLAILGYGAKWGYENATMGSTKPSGEAC
                     VMTDVGDELTPRKVTVRVFNGGTAPKAASLTKLYLNSHDFRVIRTGNSERAVVNTVII
                     GNSPDDPEVKLVQQYFADAVAEGDGRADHVVDVILHDKTKRATKIAGSVPVDGPICLP
                     RVSATPSASPTPTK"
     gene            complement(7164..8645)
                     /locus_tag="PKJ61_08545"
     CDS             complement(7164..8645)
                     /locus_tag="PKJ61_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HOC13911.1"
                     /translation="MGLDQILLLGSVVLLVSIGAARLGTRFGLPSLLLFLGLGVLVGE
                     LGLDFNDADLAHSLGFAALVLILAEGGFTTKWAEIRGALPAAILLATVGVGVSVAIVA
                     AFGHFVLGLSPATAVLLGAITSPTDAAAIFSVLRKVPLPAKLRATIEAESGLNDAPIV
                     LLVATATSWALGQGVEGGPVWMVTLIVLELAGGVVLGAALGWVGVQVLRNIALPASGL
                     YPLAALGWAVLSYALGSSLHLSGFAAVYVTALVLGHGELPHRHATRSFAEGVGWIAQI
                     GLFVMLGMLADPFRVTWANVLAGVAAGAVLTFVARPLSVLVCMVWFRTPWREQAFVSW
                     AGLRGAVPIIMATVPLAAGAPGASQVFDIVFAFVVVFTLLQSPTLPWASRLLKVSTGE
                     DATDVEVEFAPLDNIRADMMQVHVSEGSRLHGVTVRELRLPKNSVISLIIRDNEPFTP
                     SGEQIVRAGDDLLIVTNSQDRRKVEQRLRSISVGGRLARWQDG"
     gene            complement(8764..9153)
                     /gene="dhaM"
                     /locus_tag="PKJ61_08550"
     CDS             complement(8764..9153)
                     /gene="dhaM"
                     /locus_tag="PKJ61_08550"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: protein motif:HMM:TIGR02364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HOC13912.1"
                     /translation="MSVAFVIVSHSDKLAEGVVDLAGQMARDVQLRAAGGTDDGRIGT
                     SFDKVSAAVAELRASGHDVAILTDLGSANMTVEAVVDFLEPEDAAHVRFCDGPLVEGA
                     VAAAVTAQVGGDLDAVQAAARARELFG"
     gene            complement(9150..9794)
                     /gene="dhaL"
                     /locus_tag="PKJ61_08555"
     CDS             complement(9150..9794)
                     /gene="dhaL"
                     /locus_tag="PKJ61_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011183952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HOC13913.1"
                     /translation="MSETLSVAWALAWIRGAQAVLADHRMELIDLDRAIGDGDHGENM
                     DRGFKAVVAKLDPASGNPEPASVQDVLKLVASTLMSTVGGAAGPLYGTAFLRAAKAVD
                     GEVDADAVVALIAGGLEGIVARGKATTGEKTMVDAWTPAHEAAKEAALAGAAPAAVLR
                     AAADAATAGAEATIPMLATKGRASYLGERSIGHKDPGATSTAYLLDAAAKAAGA"
     gene            complement(9799..10800)
                     /gene="dhaK"
                     /locus_tag="PKJ61_08560"
     CDS             complement(9799..10800)
                     /gene="dhaK"
                     /locus_tag="PKJ61_08560"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HOC13914.1"
                     /translation="MKKLINDPANVVAETLAGFQAAHADLVTVHFDPDYVVRADAPVQ
                     GKVGLVSGGGSGHEPLHAGYVGKGMLDAAVPGAVFTSPTPDPILEATKAVDGGAGVLH
                     IVKNYTGDVLNFETAAEMAEMEGITVKAIVVNDDVAVEDSTWTAGRRGVAGTVLVEKI
                     AGAAADRGDDLDAVLAVAEKVNAQVRSMGVALTACTVPHAGKPSFDLTEEEIEIGIGI
                     HGEPGRHRIPMAPADSITDQLVDPILTDLAAPSGSKVLLFVNGMGGTPEAELYIVYNH
                     ARKRLEDAGMEVTRSLVGNYITSLEMQGCSITVLLLDDELTALWDAPVYTPALRRGL"
     gene            complement(11259..12092)
                     /locus_tag="PKJ61_08565"
     CDS             complement(11259..12092)
                     /locus_tag="PKJ61_08565"
                     /inference="COORDINATES: protein motif:HMM:NF013070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HOC13915.1"
                     /translation="MEIARVFNNNLVLALDEVGREVILTGRGLGFQARPGQVVDAAKV
                     VRVFVPTDGRDPDHLAQLLAGIPPEHIALVGAALTEVGLDRLGGNPALVIALADHVSF
                     ALKRVSVGMELEYPLLAEVQHLYAEEYAKAQALLAAVNARSAVKLPGIEAVGLALHLV
                     NAGFATGDLSYTYKMTGVIQQMVAVIERTFGLDLDSGSVSVGRFITHLRYLFVRIHNH
                     KQLSQEHSDVGAAIRAAYPDAEACALRLAGLLELRLGSRLTDDEVSYLALHVARVTAD
                     F"
     gene            complement(12159..14228)
                     /locus_tag="PKJ61_08570"
     CDS             complement(12159..14228)
                     /locus_tag="PKJ61_08570"
                     /inference="COORDINATES: protein motif:HMM:NF014437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HOC13916.1"
                     /translation="MESTSTTSTAEEIVAKVGGPGNILSLTHCATRLRFELQDASLVD
                     QAAVEKIKGVMGAVPQSGDRFQVVIGGAVQTTFNQIMNLPSMSGVGKGLSDADVKARE
                     RAKARGKVAWLDAFFEYLSDSFRPLLGVLLGASLIIAFAAVLDALGLVDFRSADKAAG
                     WFFVDAMWRSVLYFLPIMVAYNAAKKLNIDPWLGATIMAALFTPEFLMLSDPTAIPST
                     VCTTNPTLGTDSCIADIFGLPMQLNGYGGQVFVPLMMVAVLAVVYRLLKKIFPDNIQM
                     VFVPFFSMLIMIPVTAFLIGPLGIWLGTGIGTGLAWLNGNAPIVFAILIPMIYPFLVP
                     LGLHWPLNALMLANIASPATGHMDFIQGPMGSWNFACFGATAGVLLIAARNRNREMRE
                     VATGALAAGLLGGISEPSLYGIHLRYKRIYPRMLVGCAVGGILTGLLGWSSGGVKTDV
                     FAFTSLLTIPVFTPMWVYVISITAAFVTAMVLIVISDYRTPEQKAEEEAERAQEAADL
                     ALEKARLSGAAAPVAAATTAAVGGGVATLVRTDVIGAPVTGQVVPLEQVNDKVFASKA
                     LGEGVGIVPTDGHVIAPVGGVLITVPESGHAFGIRTDDGVEVLVHVGIDTVRLEGKGF
                     QVEVKADQRVEAGDLLAKVDLDVVKSAGYDPTTIVVVINTMSLTSVAPRNAGKVGLGD
                     PIIDVKP"
     gene            complement(14486..14573)
                     /locus_tag="PKJ61_08575"
     tRNA            complement(14486..14573)
                     /locus_tag="PKJ61_08575"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14537..14539),aa:Ser,seq:cga)
     gene            complement(14760..15212)
                     /gene="tadA"
                     /locus_tag="PKJ61_08580"
     CDS             complement(14760..15212)
                     /gene="tadA"
                     /locus_tag="PKJ61_08580"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008354659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HOC13917.1"
                     /translation="MNRWEPAMAQALAEAGPASAHGDVPIGAVVLDGSGAVIARAHNE
                     RELTGDPSAHAEVLALRRAAASVRSWRLDDCTLVVTLEPCTMCAGALVLARVKRLVFG
                     AYDPKAGAVASLWDVVRDPRLNHRVEVVGGISADACGAPIQEFFKAQR"
     gene            complement(15240..15845)
                     /locus_tag="PKJ61_08585"
     CDS             complement(15240..15845)
                     /locus_tag="PKJ61_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine deaminase-associated protein"
                     /protein_id="HOC13918.1"
                     /translation="MTTSDDFEIELPNRGDDRPGIDDLEDLEAEAAEDDDLDDYPDDA
                     TEDDIDLVVALYREDGAPVALALDYDLANDLEALITQLRRIPGDGGAVGLVSIASEVF
                     LIVRVRGKHVQVFCSDALAAADWPLARDVVDLLGVDVPEDEEDSGYIGDLDILADAGL
                     HDFELEAMADDFDEDAAVLVERIATRIKFGPEFAKALASFE"
     gene            15966..>16225
                     /locus_tag="PKJ61_08590"
     CDS             15966..>16225
                     /locus_tag="PKJ61_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002534602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HOC13919.1"
                     /translation="MDHPLVAHKVSLLRDVRTASATFRQLVEELVTLLAYEATREVRV
                     EEVDVVTPITTTKGVELTRPRPLVVPILRAGLGMLEGMMRLMP"
BASE COUNT         2741 a         5503 c         5402 g         2479 t
ORIGIN      
        1 agtcgtcgta caagaacgtg ctctgcctgg gccacatcct cgccgaggac ggccgcaaga
       61 tgagcaagca cttgggcaac atcctggagc cgatcccgct gatggacacc cacggcgcgg
      121 acgcggtgcg ctggttcatg gccgccggcg gctcgccctg ggcgtcgcgc cgggtcggtc
      181 acggcactat ccaggagacc gtccgcaagg tgctgatgac cttcctgaac acggtcgcgt
      241 tccagagcct gtacgcccgc gcgaacgact ggtcgccggc atcgacggct gcccctggcg
      301 agaccgagca cgttctcgac cgctggctgg tcagcacacg caacacgctc accaagcagg
      361 tcaccgaagc gctggaggac ttcgacactc aacgggcggg cagcctgctg gccgcgtttg
      421 tggacgacct gtccaactgg tacgtccggc gctcacgtcg ccgcttctgg gacggccagc
      481 cgggcgccct gcagaccctg cacgacaccc tcaacgtggt cacccggctg ctggctccgc
      541 tggtgccgtt catcaccgag cgggtgtggc aggacctttt cgtcaccacg gactcggccg
      601 cgccggcctc ggtgcacctc gcgtcctggc cggaggcgga cgctgccgcg atcgacgcga
      661 agctggacga ggcgatggcc gtcacccgcc ggatcgtcga actcggccgc tccgcccgcg
      721 ccgaggccaa ggtgaagacc cgccagccgc tgcgtcgggc gctgattccg tccggtgcgt
      781 tcaacctgct ggacgcggag ttgcgcgccg aggtgatggc cgagctgaac gtcgagcggg
      841 tggagtcgtt cgcgtccgcc ggtgacctcg tcgattactc ggccaagggc aacttccgta
      901 acctcggcaa gcgcttcggc aaggacaccc cgctggtggc atcggccatc gcgtccgccg
      961 atgccgctac gctttcggcc gacctggcca cgcacggccg cgccgaggtg ctggtcgccg
     1021 acctgggtgc ggtcgaggtg acccccgagg atgtgatcgt gtccgagcgt ccgcgggagg
     1081 gctggtcggt ggtcaacgag cagggtgaga cggtcgcgct cgacctggag ctcactcctg
     1141 agctggtgcg ggccggcctg gcccgcgagg tgatccgggg cgtgcaggag gcgcgcaagt
     1201 cgtccgggtt cgaggtggct gaccgcatcc ggctcgcctg gcaggccgac gacgaggcca
     1261 ccgcggacgc cgtccgcgcg cacgccgagt tcatcggtgc cgaggtgctg gcggtcgagg
     1321 tcgccgaggc ggaaccggcc gacgattggt tcgccgacgc cgaactcggc ctgcggttcg
     1381 cggtcacccg ggtcgagcag tcctgatgtc cgggcccggc ggcagcgagt cgcgactcac
     1441 gccggtcgtg ctgctgcatg ctgcgggact caccccgcag atgtggcagg cacaggtcga
     1501 ggctatcgct gcccaggccg aaccggccca acgcccgact cagggagcgg aggtcggggc
     1561 agcccaacct gcggagcgcc aggtgatggc gccgtggatc gcgggcctgc gtccggggcg
     1621 tccgggtgag ttgtcgctga ccggcgcggc ggcccaactg gtcggcacgc tggacagcta
     1681 cggcatcgag caggcgatcc tggtcgggca ccaactgggc gcgatggtcg ccctgcaggt
     1741 cgcggcgacc gagccggagc gggtggccgg cctggtgcta tccggcgcca tggccgcgcc
     1801 cggacgggtg gcgctggcca tgcagaaggg cgtgatccgg atgctgccca atcgcaccct
     1861 tgctgactcc ggcgcgtcca aggctgatct gatcaaggcg ctggacgtga tcgcgtccgc
     1921 cgacttcggg aagctggagc aggtgaacac tcgggcgctg ctgatcgccg gggccaacga
     1981 tccgctgctg gcggccaccc ggcagctcgc gtctcggctg ccgcaggcgc gtctggttga
     2041 gttgtccggc gcagggccgc accccaacgt cgagcagccg gacgcctaca accggctgct
     2101 ggcagacttc ctggcatgga cgccgccggc ctgacccaac gcctcgtcaa gcggacgcgc
     2161 ggtcgtccgg gcgcgctggc gctgatcacg gcggacggca tcacgacggt ccgccacggc
     2221 tgcggccccg aagctgactt cgagatcggc tcgatcagca agggcgtcac cggactcctg
     2281 taccactgtg cgctggagcg cggcgaggtg accgccgata cccagctcgg ccaactgctc
     2341 ccgctggccg acagcccggc cgcggcgatt cgcctggccg acctagccac gcatcggtcg
     2401 ggactgccgc ggannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngaactgc
     2521 taccgacctc ggtccctgga tcggctgggc gacgccccac ccggccctgg gtcggagtca
     2581 gcctcggccc ggccggtggc atccgggcgt ccgtcgaagc gatggcgaag ctggcgcagg
     2641 cgctgctgat gcgcacgact tctacaacgc cgtgctgaag aagggaaaga ccgaccctgc
     2701 gcaggaggtg aaggtagccc ggatcaccgc tgtggtgatc ggcgtgctgg caatcgtcgg
     2761 tggcatcgcg gccaacggcc agaacatcgc gttcctggtg tcgctggcgt tcgcggtggc
     2821 agcgtgcgcc aacctgccct caatcctgat gaccctgtac tggaagcggt tcaccacccg
     2881 gggctcggtc tggtcgatct acaccggact gctcggctcg atcctgctga tcgtgttcag
     2941 cccggccatc tccagcccga cctggaccaa cccggtggcc tggttcccgc tgaccagccc
     3001 caccatcgtg gcgctgccgc tggccttcct cgccggcatc atcgggacgc tcacctcgag
     3061 gtcgccggcc gatccggaac tgcaggccga gatggaggtc cgctcattga ccggagccgg
     3121 cgtggcggag gtcgtcgcgc actgatcagc ccgcacgcac gaaacagccg gcccaccctg
     3181 ggatgagccg gctgtttcgc gtccgcagtg cgggactagg gtgttcaggt gccggacgcc
     3241 aaccaagcca agctcgactc ccgcggactc ctgctggctt tcctcgccta cctcctgtgg
     3301 ggtgcgttcc cgctgtactt ccacctgctc agcgcggccg acccggtgga gatcatcggc
     3361 caccggctgg tctggaccaa cgtgttctgc ctggccgggg tgttggtttg gcgcgaatgg
     3421 acgcatctgc gggcagtcgt cgccgaccgg aagctgttct ggcgactgac cctggccggc
     3481 gtcctggtca gcgccaactg gctgatctac gtgtggggcg tgctgaacaa ccacatcgtg
     3541 gacgctgcgc tcggctactt catcaaccca ctgttcacgg tagcgctggc ggtcttcgtc
     3601 ctcggcgaga agctgcgccg cgcccaactg gttgcagtgg cgatcggcat ccttgcagtg
     3661 gtggtgatcg cggtcggcta cggccagatc ccgtgggtgg cactgtcgct ggcggtcacc
     3721 ttcggcatct acggcctggt caagaagcag gtgggcggac gcgtctcacc gctggtcggg
     3781 ctcaccgtcg aaaccaccat gctggccccg gtcgccctgg cctacctggt ctggctgcag
     3841 gccagcggac acggtgccta cctggcccac gggccaggac tgaccgtcgc gctcacgctc
     3901 gccggcgcgg tgaccgcgat cccgctgctg atgtttgcgg ccgcctccag ccggatcccg
     3961 ctgagcatga tgggcttcat ccagtacctc acgccggtga tccagttcgc gctcggggtg
     4021 tgggtgaacc acgaggagat gccgctcgcc cgctggctgg gattcgggct ggtctggctg
     4081 gctgtggtcg tgctcaccgt tgacggcgtg cgcgccgctc ggcgtccgga catcatcgag
     4141 cccgaggtcg tgaactgagc tcgcgcaccg accggtcccg gcccgtccga tcaccgagag
     4201 cccgtccgat caccgagagc cagcctcgga ctctgagcgc cagccttagg ggctagtcct
     4261 cggcgctcaa ccctggttct cggccggggg cgggacgcgc atcctgatcg acgcgaccgg
     4321 ccacgagctc cggcggcggg ctgaatcgcg cagtcgccgc aacgacccgg ggcaccggac
     4381 aggtcagcgc aggtcggacg gaggaccgcc cagctcggcg ggccggcggc gacgcctcag
     4441 ctccttggtg atctgaccga gcacgatgct cgcgatcacg gccatgaaaa tgttgccgat
     4501 gaacgaacca atgccgagaa tccccaggaa gatcgcgatc ggccagatca cgccattaac
     4561 cacgttcagc gttgactcca gcacgctggg cgcgggcggc gcctcgcccg gcaccaccac
     4621 gtgcccgctg acgacggggg cctgattggg atcgttcgtt gcggcgaacg gcggctgctg
     4681 ccaactgccc tgggattcgt ccggtgtggt ctggctcacc cagccatcgt acgttcaccc
     4741 atccgacggc acccggcgca accacggacg ccgtccagcg agcatgggca aggctgggca
     4801 gcacgtcgcc gctctggcaa agtaataccg gctccgggcg gcgcagagcg cctcggacaa
     4861 acacacagga gacgacaagt tgcgggtggt acagaagttc ggcggctcct cggtcgccga
     4921 cgccgagagc atcaagcggg tcgcgcgccg gatcgtggcc acccagtccg cgggcaacga
     4981 ggtggtcgtg gtcatctcgg ccatgggcga cacgaccgac gacctgatgg acctcgccca
     5041 gcaggtctcg ccgaacccgc gtccgcgtga gctggacatg ctgctcaccg ccggcgagcg
     5101 gatgagcgca gcgctgctgg cgatggcgat caacgacctg ggcgcggacg cccggtcgtt
     5161 caccggctca caggccggcg tgatcacgac cgggacgcac ggcgacgccc ggatcatcga
     5221 catcaccccg ggacgcatcg tctcggcgct cgaggacgga gacattgtga tcgtggccgg
     5281 cttccagggt gtctcccaga ccaccaagga cgtcaccacg ctgggacgcg gcgcgtccga
     5341 caccaccgcg gtcgcactgg ccgcagcgct ggacgccgcc tactgcgaga tctactcgga
     5401 cgtggacggc atcttcaccg ccgatccccg gatcgtgccg ggcgcgcgcc agattccgca
     5461 gatcggctac gaggacatgc tcgagatggc agccaacggc gccaagatcc tgcatctgcg
     5521 ctgcgtggag tacgcccgcc gcgagcacgt acccgtccat gtgcgctcgt ccttctcgga
     5581 caagcccggg acgtgggtca aggacatcaa ctacgaggag tcaggcatgg agcagccgct
     5641 gatcgagggc atcgcgcacg accgcagcga ggcgaagatc acgatcatcg gggtccccga
     5701 ccgggtcggc gaggccgccg agatcttcac cgtggtggcc gaagccgacg tgaacatcga
     5761 catgatcgtg cagaacgtgt cccgcgtgca ggagcaggcc accgacatct ccttcaccct
     5821 cgcgcagcag gacggcgaca aggcgatcgc cgcgctgcgc gcagtgcagg agcggatcgg
     5881 cttcctggat gtgctctacg acgaccaggt gggcaaggtc tccgtggtcg gcgtcggcat
     5941 gcgctcgcac cccggcgtca ccgcgcgctt cttccgggcc ctcgctgagg cgaacgtgaa
     6001 catcggcatg atctccacct ctgagattcg catctcggtc gtggtggcga tcgacgacgt
     6061 cgaccgcgcc gtccgcgccg gtcacaccgc cttcggcctc gactccgacg agcatgcggt
     6121 cgtctacggc ggcaccggcc gctgaacgaa aggggacggt tcctttttcg ttcaggttat
     6181 ccacaggctg tcgcccgatt cgtcgccgca tccctgtgga caagttggat ggaaagagaa
     6241 ccgtcccctt tccgttcaag ctcgggaggc tcggcacgta agctgaaggg gtgatcttca
     6301 agcgcgtcgg cgactcccgt ccctacccgg agcacggtta cgtgcccaag caatgggctg
     6361 cgatcgcacc gcagcaggta cggctcgacc agttggtcac caccaagcgg acgctcgacc
     6421 tcgacgccct gctcgaagag gactccacct tctacggcga cctgttcgcc catgtcgtgg
     6481 cgttccaggg cgagctttac ctcgaggacg ggctgcaccg ggccctgcgc gccgcgttgc
     6541 agcagcgcca gaccgtgcac gcccgggttc tggaactcaa gtaggctggt cggacagttc
     6601 gtccacagac agcaggagca cccgtgcggc agttcatccg ggcaatcaag accccggtca
     6661 cgctgatcgt gttgctcgcg atcctcggct acggcgccaa gtggggctat gaaaacgcga
     6721 ccatgggctc gaccaagccg agcggcgagg cctgtgtgat gaccgacgtc ggcgatgagc
     6781 tcaccccgcg gaaggtcacc gtgcgggtgt tcaacggagg caccgctccg aaggccgcga
     6841 gcctcaccaa gctttacctg aacagtcacg atttccgggt gatccgcact ggaaacagcg
     6901 agcgtgcggt ggtgaacacc gtgatcatcg gcaactcgcc cgacgatccc gaggtcaagc
     6961 tcgtccagca gtacttcgcg gacgcggtcg ccgagggcga cggacgtgcc gaccacgtgg
     7021 ttgacgtaat cctccacgac aagaccaagc gggccaccaa gatcgccggg agcgtccccg
     7081 tcgacggccc gatctgtctg cccagggtca gtgcgacacc ttccgccagc ccaactccca
     7141 ccaagtagcc gaggctccgg aggctagccg tcctgccagc gcgccaggcg tccgcccacg
     7201 gaaatgctgc gcaggcgctg ttcgaccttg cgccggtctt gggaattggt cacgatcagc
     7261 aggtcgtcac cggcccggac gatctgctcg ccgctgggcg tgaacggctc gttgtcgcgg
     7321 atgatcagcg agatcaccga gttcttcggc agcctcaact cgcggacggt gaccccgtgc
     7381 agccgcgagc cctccgagac gtgcacctgc atcatgtcgg cgcggatgtt gtccagcggg
     7441 gcgaattcga cctcgacgtc agtggcgtcc tcgcccgtcg agaccttcag cagccgcgac
     7501 gcccagggca gggtcggcga ctgcagcagt gtgaacacca ccacgaacgc gaagactatg
     7561 tcgaatacct ggctggctcc gggcgcgccg gcagccagtg ggacggtggc catgatgatc
     7621 ggcaccgcac cgcgcagccc ggcccaggac acgaaggcct gctcgcgcca gggcgtccgg
     7681 aaccagacca tacagaccag cactgacaac ggcctggcga cgaacgtgag caccgccccg
     7741 gcggcgacgc cggccagcac gttcgcccag gtgacccgga acgggtcggc gagcatgccg
     7801 agcatcacga agaggccgat ctgcgcgatc cagcccaccc cttcggcgaa cgatcgggtt
     7861 gcatgccggt gcggcaattc accgtggccg aggacgagtg cggtcacgta cacggccgcg
     7921 aacccggaca gatgcagcga gctgcccagc gcgtaggaga gcaccgccca gccgagtgcg
     7981 gccagtggat agagcccgga cgccggcagt gcgatattcc gcagcacctg gacgccgacc
     8041 caccccagcg ctgcgccaag gacgacaccg ccggcgagtt cgagcacgat cagggtgacc
     8101 atccagaccg gcccgccttc gacgccctgg ccgagcgccc agctggtcgc ggtcgcaacc
     8161 aggagcacga tcggggcgtc gttgaggccg gattcggctt caattgtggc ccggagcttg
     8221 gccgggagcg gcacttttcg gagcacggag aagatcgcgg ccgcgtccgt gggagaagtg
     8281 atcgcgccca ggaggacggc ggtggctggg ctgagcccga ggacgaaatg cccgaaggcg
     8341 gccacgatgg ccaccgagac gcccaccccg accgtggcca gcaggatcgc cgcgggtagg
     8401 gcgcccctga tctcggccca tttggtggtg aaaccacctt cggcgaggat cagcaccagc
     8461 gccgcgaacc ccaacgaatg ggcaagatct gcgtcgttga agtccagccc gagttcgccg
     8521 acgagcacgc cgagtccaag aaagagcagc agcgatggca acccgaaccg ggtgccgagc
     8581 cgcgcagcgc cgatcgatac gagcaggacg acagacccca acaacaggat ctgatccaag
     8641 cccacgcgcg ccccctttgc cgcagcttat cgacttcgct cccgaatggg accctcgctc
     8701 ccgaactatc gggagcgagg gtcccgttcg ggagcgaagt tgggcggacg gaacttgtcg
     8761 ggcctagccg aacagctcgc gggcgcgcgc tgctgcctgg acggcgtcca ggtcgccgcc
     8821 cacttgggcg gtgaccgcgg cggcgacggc accctcgacc agtgggccgt cgcagaaacg
     8881 gacgtgggcc gcgtcctcgg gttcgaggaa gtcgaccacc gcttcgactg tcatgttcgc
     8941 cgagccgaga tcggtgagga tcgccacatc gtgaccggac gcacgcaact cggctaccgc
     9001 agccgacacc ttgtcgaagg aggttccgat gcgaccgtcg tcggtgcccc cggcagcccg
     9061 caactgcacg tcacgggcca tctgaccagc gaggtcgacc acgccctcgg ccaacttgtc
     9121 cgagtgcgag acgatcacaa aggcgacgct catgctccgg cggccttcgc ggcagcatcc
     9181 aacaggtagg ccgtggacgt ggcgcccgga tccttgtgcc cgatcgatcg ctcgccgaga
     9241 taggacgcac gtcccttcgt ggcgagcatc gggatggtcg cttccgcacc ggctgtggct
     9301 gcgtccgctg ctgcccggag cacggccgcc ggtgccgccc ccgcaagggc agcttccttg
     9361 gccgcctcgt gggccggggt ccaggcgtcc accatggtct tctcaccggt ggtcgccttg
     9421 ccgcgggcaa cgatcccttc cagcccgccg gcgatcagcg cgacgactgc gtccgcgtcc
     9481 acctcgccgt cgacagcctt ggccgcacgc aggaacgccg tcccgtagag cggaccggca
     9541 gcaccgccga cagtcgacat cagggtggac gcgaccagct tcagcacgtc ctgcaccgac
     9601 gccggctccg ggttcccgga cgcggggtcg agcttggcca cgaccgcttt gaagccgcgg
     9661 tccatgttct cgccatggtc gccgtcaccg atggcacggt cgaggtcgat cagttccatt
     9721 cggtggtcgg ccaggacggc ctgggcgccg cgaatccacg cgagcgccca ggccaccgac
     9781 agcgtttcac tcaccggctc agagcccacg acgaagggcc ggggtgtaga ccggagcgtc
     9841 ccaaagggcg gtcagctcgt cgtccagcag caggacggtg atcgagcagc cctgcatctc
     9901 cagcgaggtg atgtagttgc cgaccagcga cctggtgacc tccatcccgg cgtcttcgag
     9961 gcgcttgcgg gcgtggttgt agacgatgta gagctcggcc tcgggggtgc cacccatacc
    10021 gttcacgaac agcagcacct tcgagccgga cggggccgca agatcggtca ggatcgggtc
    10081 gaccagctgg tcggtgatcg agtccgccgg agccatcggg atacggtggc ggccaggctc
    10141 gccgtggatg ccgatgccga tctcgatctc ctcctcagtt aggtcgaagg acggcttgcc
    10201 ggcgtgcggc acggtgcacg cggtgagcgc gacacccatc gaacgcacct gcgcgttgac
    10261 cttctccgcg acggccagga cggcgtccag gtcatcgccg cgatcggcgg ccgcaccggc
    10321 gatcttctca accaggacgg tgcccgcgac gccgcggcgt cccgcggtcc aggtggaatc
    10381 ctcgacggca acgtcgtcgt tgaccacgat cgccttgacg gtgatgccct ccatctcggc
    10441 catctcggct gcggtctcga agttgagcac gtcgccggtg tagttcttca cgatgtgcag
    10501 gacgcccgcg ccaccgtcga cggccttggt ggcctcaagg atggggtcgg gggtcgggga
    10561 ggtgaagacc gccccgggga cggccgcgtc gagcatgccc ttgccaacgt agccggcgtg
    10621 cagcggctcg tggcccgatc cgccgccgga caccagaccg accttgccct gaactggcgc
    10681 gtcggcgcga accacgtagt caggatcgaa atggacggta acgaggtcgg cgtgtgcagc
    10741 ctggaaacca gcgagggtct cggcaacaac attggccgga tcgttgatca gtttcttcac
    10801 tccagctctc ctttgaggcg cgttgtggtg ggcagtccca actttgcccg caaagcccgg
    10861 ggtcgggaag gggcagggct gcgcaccttc gttcaccgcc gaccgctcct gctgaacgaa
    10921 agcctgcgca ccgggacacc ggcgtcccgg agcccggtca acgcagtccg cgggtttgaa
    10981 cccgattccg accggttatg aaggcatcgg ccgcaccgcc gaacggttca acccgaaaac
    11041 gacaggttat gtaggccaga tcgggcttca taacccgcag aaattgggtt gaccgggctc
    11101 gggtccggaa accgggcttc ataaccggtc aaaatcgggt tgaacctccc acgaatcggt
    11161 gcgtgaccac ggagagtgcc gcgtgcgggt ttgagcttgt cgaaacccgc cggcaacagc
    11221 cgactcgaca agcgcgacca gccgatatcc agcggagttc agaagtcggc ggtcacgcga
    11281 gccacatgaa gggccagata ggagacttcg tcgtcagtga gccgggagcc cagccgcagt
    11341 tccagcaggc cagccaggcg caacgcgcag gcctccgcgt ccggataggc cgcacggatc
    11401 gccgcgccaa cgtccgagtg ctcctggctg agctgcttgt ggttgtggat gcgaacgaag
    11461 aggtagcgca ggtgagtgat gaaccggccg acgctcacgc tgccggagtc caggtcaaga
    11521 ccgaaggtgc gctcgatgac cgcgaccatc tgctggatca cgccggtcat cttgtaggtg
    11581 taggacaggt caccggtggc gaaacccgcg ttcactaggt gcaacgcgag ccctaccgcc
    11641 tcgatgccgg gcaacttgac cgcgctgcgg gcgttgacgg ccgcgagcag cgcctgcgcc
    11701 ttggcgtact cctctgcgta gaggtgctgc acctcggcca gcagcggata ctccaactcc
    11761 atccccaccg acacgcgctt gagcgcgaaa ctcacgtgat cggccagcgc aattaccagc
    11821 gcagggttcc cgcccagccg atccaggccg acctcggtga gggcagcgcc gaccagggcg
    11881 atgtgctccg gcgggatccc ggcgagcaac tgcgccaagt ggtcagggtc gcgtccgtcc
    11941 gtcggcacga agacccggac gaccttcgca gcatcaacga cctgacccgg ccgggcctgg
    12001 aaccccaggc cacggccggt caggatgacc tcgcgtccga cctcgtccag cgcaaggacg
    12061 aggttgttgt tgaagacgcg cgcgatctcc acgtcagccc cgggcgggac cgaactggga
    12121 cgcccgtcgg gtgggcttgg cgatcacggc gactcccctc acggtttcac gtcaatgatc
    12181 ggatctccga gacccacctt gccagcgttt cgcggtgcca ccgaggtgag cgacatggtg
    12241 ttgatcacga ccacgatggt ggtggggtcg taaccggccg acttcaccac gtcgagatcg
    12301 accttggcca gcaggtcacc ggcttccacc cgctggtcgg ccttgacttc cacctggaag
    12361 cccttgcctt ccagccggac ggtgtcgatg ccgacgtgca ccaggacttc gactccgtca
    12421 tcggtgcgga tgccgaacgc gtggccagac tccgggacgg tgatcagcac gccgcccacc
    12481 ggggcgatca cgtggccgtc cgtcggaacg atacccacgc cttcacccag cgccttggac
    12541 gcgaacacct tgtcgttcac ctgctccagc ggcaccacct ggccggtgac cggggcgccg
    12601 atgacgtccg tccgcaccag ggtggcaaca ccaccaccga ccgcggcggt ggtcgcggcc
    12661 gccaccgggg cagcagcacc ggaaaggcga gccttctcca gagccagatc ggccgcctcc
    12721 tgcgcccgct cagcctcctc ctcggccttc tgctccgggg tgcggtagtc ggagatcacg
    12781 atcaggacca tcgccgtgac gaaggccgcg gtgatcgaga tcacgtacac ccacatcggc
    12841 gtgaacaccg ggatggtgag cagcgaggtg aaggcgaaca cgtcggtctt cacgccgcca
    12901 ctggaccagc cgagcaggcc ggtgaggatg ccgccgaccg cacaacccac gagcatccgc
    12961 gggtagatgc gcttgtagcg caggtggatg ccgtacagcg acggctcgga gataccaccg
    13021 agcagacccg cagcgagggc gccggtggca acctcgcgca tctcccggtt gcggttgcgt
    13081 gcggcgatca gcaggacgcc ggccgtggcg ccgaaacagg cgaagttcca cgaacccatc
    13141 gggccctgaa tgaagtccat gtggccggtg gccgggctgg cgatgttggc cagcatgagc
    13201 gcgtttagcg gccagtgcag gcccagcgga accaggaacg ggtagatcat cgggatcagg
    13261 atcgcgaaca cgatcggggc attgccgttc agccaggcca ggccggtgcc gatgccggtg
    13321 cccagccaga tgccgagcgg gccgatcagg aacgcggtca ccggaatcat gatcagcatc
    13381 gagaagaacg gcacgaagac catctggatg ttgtccggga agatcttctt cagcagtcgg
    13441 tagaccacgg cgagtaccgc gaccatcatc agcggcacga acacctggcc gccgtagccg
    13501 ttcagctgca tcggcagtcc gaagatgtcg gcgatgcagg agtcggtgcc cagcgtcggg
    13561 ttggtggtgc agacagtgga cgggatcgcc gtcggatcgg acagcatcag gaactcgggc
    13621 gtgaacagcg cggccatgat cgtcgcgccg agccacgggt cgatgttcag cttcttggcc
    13681 gcgttgtacg ccaccatgat cggcaggaag tagagcaccg agcgccacat cgcgtccacg
    13741 aagaaccagc cggccgcctt gtcggcggaa cggaagtcga ccaggccgag ggcgtccaga
    13801 accgcggcga aggcgatgat cagggacgcg ccgagcagca cgccgagcag cggacggaac
    13861 gagtcggaca ggtactcgaa gaaggcgtcc agccaggcga ccttgccacg cgccttggcc
    13921 cgctcgcgag ctttcacgtc cgcgtcggac aggcctttgc cgacgccgga catggacggc
    13981 aggttcatga tctggttgaa ggtggtctgg acggcaccgc cgataaccac ctggaagcgg
    14041 tcaccgctct gcggcaccgc acccatgacg cccttgatct tctcgaccgc agcctggtcg
    14101 accaggctcg cgtcctgcag ttcgaagcgc agccgggtgg cgcagtgggt aaggctcagg
    14161 atgttgccgg gaccgccgac cttggcgacg atctcctcgg ctgtgcttgt tgtggatgtg
    14221 gactccatcg tttcctcttt ccgctcacca gtcgttgtgt gccgaaaaac ccattgtgtt
    14281 tcccgaagag gcactatgtg ccgaaaaaca aaaaagaccc gagacgcatc aagatgcatc
    14341 tcaggtcttg cccctcgcgg gtaacaaccc tgtcgagcca gctggctcgg ccggaaacct
    14401 atcacagagt ggccgggtgg ttccaccaaa gttccgaaag gtgatcgccg ctgccatccg
    14461 gtggggatgg cagcggcgat cacgttggcg gtggcggagg gatttgaacc ctcggagggt
    14521 ttcccctcac gcgctttcga ggcgcgctct ttaggccgct cagacacgcc accgcggaag
    14581 agtgtagtca ttcgaagccg gagcgggcca atcgggcgga cgctccctgc ctagcgacct
    14641 caggaccaac gtttgctctc gaaaccggtg tttgctaccg aaatttcggt agcaaacacc
    14701 ggtttcggta gcaaacgcgt tctgggtgga cgtgggagtg gcgactcagc cgaggcggcc
    14761 tagcgctggg ctttgaagaa ctcctggatc ggggcgccgc aggcgtccgc ggagatcccg
    14821 ccgaccacct cgacccggtg gttcagccgc gggtcgcgga ccacgtccca gagcgaggcg
    14881 accgcgccgg ccttcgggtc gtaggcgccg aagaccagcc gcttcacccg agccagcacc
    14941 agggcgccag cacacatcgt gcacggctcc agggtcacca ccaaggtgca gtcgtccagc
    15001 cgccacgacc gcacggacgc ggctgcgcgc cgcaacgcca acacctcggc gtgcgcggac
    15061 gggtccccgg tcagctcccg ctcattgtgc gcccgagcga tcaccgcgcc ggacccgtcc
    15121 agcaccacgg caccgatcgg cacgtcccca tgcgcggacg cgggccctgc ctcagccaga
    15181 gcctgggcca tggccggctc ccagcggttc accgagggcg gttcactcga aggcggtggt
    15241 cactcgaaag acgcgagcgc cttggcgaac tcaggaccga acttgatccg ggtcgcgatc
    15301 cgctcgacca gcacggccgc gtcctcgtcg aagtcgtcgg ccatcgcttc aagctcgaag
    15361 tcgtgcaggc cggcgtccgc gaggatgtcg agatcgccga tgtagccgga atcctcctcg
    15421 tcctcgggca cgtccacgcc gagcaggtcg accacgtcgc gtgccaaggg ccagtcggcg
    15481 gcagccagcg cgtccgagca gaacacctgg acgtgcttgc cgcgcacgcg cacgatcagg
    15541 aaaacctcgg acgcgatcga gaccagcccg accgcgccac cgtccccggg gatccggcgc
    15601 agctgggtga tcagcgcctc caggtcgttg gccaggtcgt agtcgagtgc gagcgcaacc
    15661 ggcgccccgt cctcgcggta gagggccacg accaggtcga tgtcgtcctc ggttgcgtcg
    15721 tcggggtaat cgtcgagatc gtcgtcctcg gcagcctcag cctcaagatc ctcaaggtcg
    15781 tcaatgccgg ggcggtcgtc gccacggttg ggcagctcga tctcgaaatc atccgaagta
    15841 gtcaccactg cctcctagtc ccagccaaga ccccatcgtg tcagatgacg ccagttgagc
    15901 acagtccggg cgctgcgtct gcgggcgcat ggttgtggga acctagtggc gtggaacttc
    15961 acgtcatcga tcatcccctg gtagctcaca aagtcagcct gctgcgcgac gtgcggacgg
    16021 cgtccgccac tttccgccag ctcgtcgagg agctggtcac cctgctcgcc tacgaggcca
    16081 cccgcgaggt gcgggtcgag gaggtggacg tggtcacacc gatcaccacc accaagggcg
    16141 tggagctgac ccggccgcgt ccgctggtcg tgccgatcct gcgcgccgga ctgggcatgc
    16201 tcgaaggcat gatgcggctg atgcc
//
LOCUS       DAOPRQ010000059        15946 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_3088_length_15946_cov_12.879806, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000059 DAOPRQ010000000
VERSION     DAOPRQ010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 15946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15946
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3088_length_15946_cov_12.879806"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            50..769
                     /locus_tag="PKJ61_08595"
     CDS             50..769
                     /locus_tag="PKJ61_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HOC13920.1"
                     /translation="MSGLLEVIALLPADAERAEAGGADRIELCGTLDEDGLSPTPQLV
                     DQVRRATSIQLRVMVRLRAGFGTDGGEAVRLRGLMASYADAGADGMVLGFLNGLGDVD
                     VGVVTELVGDGTWPWTFHRAVDACLDADKAWAALRTLPRLDQVLTAGSARDVEHGLSD
                     LLARAKADPWAASVIMAGGGLHPDHVPWLARAGVRAFHIGKAARPGGSFKAYVDEALV
                     RSWRSLVDAEVRHRPTPAAAG"
     gene            complement(839..1753)
                     /locus_tag="PKJ61_08600"
     CDS             complement(839..1753)
                     /locus_tag="PKJ61_08600"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOC13921.1"
                     /translation="MSASIPSASTAPASAIRRRNLEHFLWLSIATAIATVLIKGYAAW
                     LTNSVGLWSDALESTVNLAAALVALWALRLSAKPADHNHDFGHGKAEYVSAAVEGTLI
                     FVAAAFIIYGAVLRLFNPVPLEQLGLGLMLSMVATLLNLGTGLLLIRAGKQYRSITLE
                     ADGKHLLTDVWTSVGVLVAIGLVALTGWHILDPLIAIAVGVNILLTGYGLVRRSVVGL
                     LDAALPEDEVAMVSAVINEVCDDPRTEITELRTRESGRQRFVYVTLTVPGTWTVKRSH
                     DVADAVEDAVSAALADTTTFVHIEPTAE"
     gene            complement(1759..2316)
                     /locus_tag="PKJ61_08605"
     CDS             complement(1759..2316)
                     /locus_tag="PKJ61_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008355250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOC13922.1"
                     /translation="MSTPFRTRRFEPVAPHLRPRRFRQAARVIVTDGVHTLMLADTDP
                     GLPGSRWWVTPGGGIDPGETPVAAAVRELVEETGLVVDPSELLGPVASRTVVHGYSDQ
                     ILSQVEHFFVVRVSEPFELSPGGLTADEQLTLDGWAWHRLAQLTDLTEPVWPANLTEL
                     AALAGRPDAWPVDLGEIEESTLPVN"
     gene            complement(2313..3092)
                     /locus_tag="PKJ61_08610"
     CDS             complement(2313..3092)
                     /locus_tag="PKJ61_08610"
                     /inference="COORDINATES: protein motif:HMM:NF023043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HOC13923.1"
                     /translation="MSSADGPQGLGTPSSDHPSFAPATGTPAASAQPLWAPPSGPAGH
                     LPSEPASFVPAPPTGRGPQRKRSTHVRLLAGGLAAALVLTALASVLPLLPRGSTIAPT
                     ASAPAPASGEPSRLVTVSPTPQPADVVDVTGPDIARAAAFTTADGSGEVTITRATWTS
                     AGELAPPEGKRYLVLDVTVTQQAGRVPVESLSFRARSADGVGVTPGFGPKLDRPLGGS
                     TLTDAGQVSGQVGFALPPGSTTIELLDAGLLPVVTLQVPGP"
     gene            complement(3092..3895)
                     /locus_tag="PKJ61_08615"
     CDS             complement(3092..3895)
                     /locus_tag="PKJ61_08615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13924.1"
                     /translation="MATWEDGPEYAPRVRPQAFLIPEAEPLAPVHALAPAPAYPEAEP
                     HFNPPSGDAPELAALVPSLAPGRNPNVPFEVVTAAVTAPGPNHAGSPSATRSPYEPFG
                     PPGPSLKGYLPVQPMVDPTAQLNPAPFPAPGTQQWFAPPPPQQAPAAPPAVTIGQIFT
                     AITPAVLITFAVGALFAWLSLFMLALAFTLSARIAYRRESVRRAFSVAFVILAAFGGV
                     SLLTDGWNADVLFDSLASGAQLACWLLPLVVFGIVGSALRAGERPDRTG"
     gene            4057..4443
                     /locus_tag="PKJ61_08620"
     CDS             4057..4443
                     /locus_tag="PKJ61_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002532933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HOC13925.1"
                     /translation="MPVGKVRYYDAEKGFGFLSKDEGGDDVYVRASALPEGVTSLKRG
                     QKVEFGVVDGKKGEQALSLRLLEAPPSLSKAARKSPEQMAIIIEDLIKMLDNVGNGYR
                     RGRHPDAKVAGKTASVLRAVADELEL"
     gene            4446..5216
                     /locus_tag="PKJ61_08625"
     CDS             4446..5216
                     /locus_tag="PKJ61_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013161683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3027 domain-containing protein"
                     /protein_id="HOC13926.1"
                     /translation="MAVALELDAACADAVELARSAAVRRAGVLEVGEHVGVIPEDTRV
                     ATHLFECVHPGYPGWQWSVTVARAARARVVTVSEVTLLPGEGALLAPAWVPWADRMGA
                     KDLTPGVLAPTPADDFRLEPGYTGGEHAAVADPAEASQTRALVAELGLGRDRVLSLAG
                     RDAAAERWLDGPGGPDTPMAQQAPALCTTCGFFVALQGDLGRLFGACTNELSPSDGAV
                     VARTHGCGGHSDVPEPTKGVDLSLPIWDTISVDVSLFD"
     gene            complement(5322..5696)
                     /locus_tag="PKJ61_08630"
     CDS             complement(5322..5696)
                     /locus_tag="PKJ61_08630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13927.1"
                     /translation="MTQNSHHHRPLMVQAPVRALDPDGVTVVTAGTVAFAIGALACWW
                     LLPELNAAGKGWYLGVSITGFVLGLCGLTFGLFRRQRRHRAELDDDLGADSPTPRYAA
                     PDELRGSDEERHSAPALGSDES"
     gene            5742..7238
                     /locus_tag="PKJ61_08635"
     CDS             5742..7238
                     /locus_tag="PKJ61_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013161681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HOC13928.1"
                     /translation="MANPGTSTAAPAPNTNKALDQWFEITKRGSNLGREVRGGLVTFF
                     TMAYIIVLNPLIIGTTPDKDGNLITGMPIADAANIPTSIAMVAAATALIAGLMTILMG
                     VYGRFPIAIATGLGLNALLAYTIAPKMTWPQAMGLVVWEGILITILVLAGFRKAIFDA
                     VPRSLRSAISVGIGLFIAFIGLVDAGVVRPGAGTPVQLGVNNQLVGWPIGIFLIGLLI
                     LIALFVRKVKGAMLIAILSATVLAVIVEAVLSLGSKSDENATGWMLNVPKLNEFAAPN
                     LGLLGRVDLFGAFTSAAGVSAVIGLIMLVFSLLLSDFFDTMGTIVAVGAEGDMLEADG
                     NPPHTQEILLVDSVSAMAGGFGSVSSNTSYIESAAGVGEGARTGFASVVTGAAFLLSL
                     VLAPLVNMVPSEAAAPALFFVGFLMMAQVVHVNWEDPEEGIPAFLTMVLMPFTYSITV
                     GIGAGFIAYALIKIIRGKAKEVHTLLYVVAAAFVLYFGQGVITALIGG"
     gene            complement(7394..9922)
                     /locus_tag="PKJ61_08640"
     CDS             complement(7394..9922)
                     /locus_tag="PKJ61_08640"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOC13929.1"
                     /translation="MNLNLTLAWRYLRGRGLRSLLTTLAVVLGVMLTFGLNGILPAMV
                     EAFTHNLLSAAGKVDLTVTSAYNQPFRADVTDRLLTVPQVATASPGVQRTVPLPRSAD
                     AAADALAQVVLVGVDVPTATAVRDFPLASGRVLAPTDRTAVVLNTDLAAELGLGLGDQ
                     LVLPAAGGTARFQVVGLLSTATLPGQEQVFVTLAAAQQLLGLGDQLTQIEAVFTPDAD
                     RAATEAAVSAALGPDYQVGGLSTESSLLASLQVAQFAFNMFGIFALATAGFIILNSFR
                     TVVAERRRDIGMLRAIGMTRRSASRLFLIESLVQGVVGTALGIAAGWAMAAGLFAAMA
                     PIFKSVMHFTIGGPLFTAATWAEAIVLGVGVTILAAVVPARAAGRVTPMEAMRPQLGE
                     VFERRVGRRAWIGAALLVASLFGLTSGSAGLVGFGSVVFLVGIALVAPAVVNPIANWV
                     SAPLESFNACEGAIARSNLQRNPGRSAVTVSAVMLGLASIVAMISVVTSIFAGFTTYL
                     DKSLSADYLVMPQSIILGQGNVAAGPRLAAQVAGTDGIAAASSLRVAQAKAAGGDVQV
                     LGIDPENYLKVASFDWNSGSSDAAVDQLRSGRWLIANGIYAAQHNLALGQQIQLDTPN
                     GARTYHVAGIGNDYLNAKLSTLYTSQELLERDFNVTADLLIMANRTPSADPTQTQARL
                     AKVVGDYPAFRLYESEAWRAEQMRTFDQTIIIFDVLVAALALPSLLALVNTLAISVLA
                     RTREIGMLRAVGATRRQVRRMVLAESLLLAVIGTGFGAIAGLWLGYSLVTAMGAVGWQ
                     MPYAFPWTGLLATVLVGLVFGVLASIGPARSASRLNVIAALRQE"
     gene            complement(9919..10665)
                     /locus_tag="PKJ61_08645"
     CDS             complement(9919..10665)
                     /locus_tag="PKJ61_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020579462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOC13930.1"
                     /translation="MTTSDRPPTVQIAGLTKIYGTGAAQVTALDAVDLSVGAGEFVTV
                     MGPSGCGKSTLLNLLGGLDAPTSGTISIDGAQLGDLPDDDLTELRRRRIGFVFQFFNL
                     IPVLNAVENASLPLRLDGVRDAESRATTWLERVGLGDRLQSRPDQLSGGQQQRVAIAR
                     ALSTEPALILADEPTGNLDSRSATEIGELLREISAAYGRSVLMVTHDPRIAGFAERLV
                     LMRDGRIIDDLDLDDAPEGPRLALERAGLL"
     gene            complement(10662..11915)
                     /locus_tag="PKJ61_08650"
     CDS             complement(10662..11915)
                     /locus_tag="PKJ61_08650"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOC13931.1"
                     /translation="MNRKTLVTTGIAAAAVLALGAGYAYSVTNSRPEVETAIAEHAEL
                     TATVSASGTLAPAHTAGVYPSAAATIAKVTVADGDTVEKGEVLATLATGPLKLAVAQA
                     RAAASAAEAQLDAVNRGVPAAIDRSAANAALSAARSQVSTAAKNYSAYLADYRDASNA
                     ERAKMRPTLRSLKTAKSTANAALKGAESGLHQLSVAARVGLARTAANDARKAADRALD
                     LAQANLDHAELTAPFAGVVSFVGTVEKGAGATPGVPVFRLVDPTRLEFQAMVNETDIA
                     GITTDQDATVSLDAFDQPFTGKVTRVHSQSETTSTGTVAFRVDVAIESSDSGALSGMS
                     GSVELATESIPDALVVPVAAVQTRGTGRTVFVVDADSVAHAREVEVGLSTDTSVQIIS
                     GLSEGDRVVTTGAAALSDGQQVRTK"
     gene            complement(11912..12616)
                     /locus_tag="PKJ61_08655"
     CDS             complement(11912..12616)
                     /locus_tag="PKJ61_08655"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOC13932.1"
                     /translation="MARITKHPLVRREELLDVAFELCRSHGFEAMSVEQVTQTAGVAK
                     GTFYHYFGSKADLQEQLVGRFGESLFNHLTEAIDGVAGDGAHRLGRLMTASAAYKSGQ
                     ADLGYAAFLYRAENFALRHRLFDAWRERARAVLEPVIAAGVADGSFHVADAGAATDLI
                     LLLWFDAADQLWRRALQSPDATAFVQVMTAGGAAISQAQERILGVPDGSYAVPIPPEL
                     VNAIEQLYSHPVRTPA"
     gene            complement(12782..13432)
                     /locus_tag="PKJ61_08660"
     CDS             complement(12782..13432)
                     /locus_tag="PKJ61_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13933.1"
                     /translation="MGSVRLFAIAITEVREFFGAPSELADRLRAIAARTTTTASVPSQ
                     GLLGKLGPVFLRQPGAPIIRPDTPGRTVLDLMLAGRHVPPERLTAAWALLGTWLAAEA
                     WGEHSAEFDAHAITALDFDLARVGVPTKWSLGDLVRNDLGLPLRPAPGVAAGYVPNAA
                     ASALAANWRPIVAELEPEHRAFVRGLLGWLDAFPEWTRQGSAAGRMPPDLVAILRS"
     gene            13632..13889
                     /locus_tag="PKJ61_08665"
     CDS             13632..13889
                     /locus_tag="PKJ61_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13934.1"
                     /translation="MIEGARRILVSMAAAFLLTLCAGVQSAFAETPTPTPVATAAATT
                     GSDTTADAPDVAPDNSAVVWALGAAGVLAVIAGAVVFLRRN"
     gene            complement(14023..14667)
                     /locus_tag="PKJ61_08670"
     CDS             complement(14023..14667)
                     /locus_tag="PKJ61_08670"
                     /inference="COORDINATES: protein motif:HMM:NF041024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acVLRF1 family peptidyl-tRNA hydrolase"
                     /protein_id="HOC13935.1"
                     /translation="MSSTTWPRTVTVPPDRLAGWLAGFAERHGTPAVEVDTDALVLQA
                     ADGAAARVRLLWGPLPEGDPLAEVVAAFARPRRVGALIVRRKAHAVGIFVGSELVAGR
                     RGSHYVQGRTKAGGWSQQRYARRRANQAERSFEAAGRDAADLLVGEAGRLDALALGGD
                     GAAVASVLALPELARLAQVERRLGPLSVPDPNARVLAEFGALFRRVPIDLNELA"
     gene            14702..15388
                     /locus_tag="PKJ61_08675"
     CDS             14702..15388
                     /locus_tag="PKJ61_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012923429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HOC13936.1"
                     /translation="MSDLIDTTEMYLRTIYELGEEGVPPLRARIAERLKQSGPTVSQT
                     VARMERDGLVSVHPDRHLVLSEHGLELAMRVMRRHRLAERLLIDVIGLEWSLVHEEAC
                     RWEHVISTDVERRLVAILDHPVMSPYGNPIPALEELDVDPAPESFLDGTELLSFRLAD
                     GQAHEVVLRRMSESIQSDPKLLAALAEAGCVPGRPVRASILGRNLLLGSQNEVIVPAT
                     VGDHLFVAPE"
     gene            15488..15886
                     /gene="trxA"
                     /locus_tag="PKJ61_08680"
     CDS             15488..15886
                     /gene="trxA"
                     /locus_tag="PKJ61_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020574061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOC13937.1"
                     /translation="MATIALTEAEFGKTITGNGIVFVDFWAAWCGPCRMFGPVFEAAS
                     EAHPDAVFAKVDTDDQRGLAGDLDISSIPTLMAFRDGYLVYREAGALNKSQFEHLVQQ
                     VKALDMEKLKAEAGLGSAADAGRDDAGDDH"
BASE COUNT         2473 a         5320 c         5673 g         2480 t
ORIGIN      
        1 acccgcgtgt ctgccgggtg cgcgggctta cgcgtcccat cattgagcca tgagcggcct
       61 gctcgaagta atcgcgttgt tgcccgcgga cgccgaacgc gccgaggcgg gcggtgccga
      121 ccgcatcgaa ctttgcggaa ccctggatga ggacggactt tcgcccaccc cgcagttggt
      181 cgaccaggtt cgccgggcga ccagtattca gctgcgagtg atggtgcggc tgcgtgccgg
      241 gttcgggacg gatggcggtg aggcggtgcg gctgcgtggc ctgatggcca gctacgccga
      301 cgctggggcc gacggcatgg tgctcggctt cctcaacggt ctgggcgacg tggatgttgg
      361 cgtggtgacc gaactcgtcg gcgacggcac gtggccgtgg accttccatc gtgcggtgga
      421 cgcgtgcctg gacgccgaca aagcatgggc ggcgctacgg acgctgccgc ggctggacca
      481 ggtgctcacc gccggttcgg cccgcgacgt ggagcacggc ctaagcgacc tgctggcaag
      541 ggcgaaggcc gatccgtggg cggcttcggt gatcatggcc ggtggggggt tgcaccctga
      601 tcacgtgccc tggctggccc gcgccggcgt ccgtgcgttc cacatcggca aggccgcccg
      661 ccccggcggc tcgttcaagg cctacgtgga cgaggccctg gtgcgttcct ggcgcagcct
      721 ggtggacgcc gaggtgcggc accgtccgac tcctgccgcg gctggctgag ccgcccagag
      781 acgtcatccc ggcgaaggcc aggatctcct ttcggcgtcg gacgctggtg gggctggcct
      841 actccgcggt gggctcgatg tggacgaagg tggtggtatc ggccagcgcg gcgctaactg
      901 cgtcctcgac cgcgtcggcc acgtcgtggc tgcgtttcac cgtccaggtg ccaggcacgg
      961 tcaaggtcac gtagacgaac cgctgccggc cggactcccg ggtgcgcaac tcggtgatct
     1021 cggtgcgcgg gtcgtcgcag acctcgttga tcaccgccga gaccatggcc acctcgtctt
     1081 cggggagcgc cgcgtccaat agtccgacca ctgagcgccg caccagcccg tacccggtga
     1141 gcagaatgtt cacaccgacc gcaatggcga tcagcgggtc aaggatgtgc cagccggtga
     1201 gcgcgaccag gccgatcgcc acgagcaccc cgaccgaggt ccagacgtcc gtcagcaggt
     1261 gcttcccgtc cgcttccagt gtgatcgagc ggtactgctt gcccgcgcga atcagcagca
     1321 acccggtgcc gaggttcagc agcgtggcca ccatcgacag catcaggccg agaccgagct
     1381 gctccagtgg caccgggttg aacagtcgca gcaccgcgcc gtagatgatg aaggcggccg
     1441 cgacgaagat gagcgtgccc tccaccgctg cggacacgta ttcggccttg ccgtggccga
     1501 agtcgtggtt gtggtcggcc ggtttggccg acaaccgcag cgcccacaac gccaccaggg
     1561 cagcagccag gttcacggtg gactccaacg cgtccgacca caggcccacc gagttggtca
     1621 gccaggcggc gtaacccttg atcagtacgg tggcgatggc ggtggcgatg gaaagccaca
     1681 agaagtgctc caggtttcgc cggcggatgg ccgaggcggg tgccgttgac gcactcggga
     1741 tggacgcact cactcgcgtc agtttaccgg gagcgtggac tcctcgatct cgccgagatc
     1801 caccggccag gcgtccggac gcccagccag cgcggccaat tcggtgaggt ttgccggcca
     1861 gaccggctcg gtcaggtcag tcaattgggc cagtcgatgc caggcccagc cgtcgagggt
     1921 cagctgttcg tcagcggtca gcccgccagg agagagctca aacggttcgc tgacccggac
     1981 gacgaagaag tgttcgacct ggctcaggat ctggtcggag tagccgtgca cgaccgtccg
     2041 gctggccacc gggccgagca actcggacgg atcgaccacg agtccggtct cctcaaccaa
     2101 ttcccgcacc gcggccgcta ctggcgtttc gccaggatcg ataccgccgc cgggtgtcac
     2161 ccaccagcgc gagccgggca gcccaggatc agtgtcggcg agcatcaggg tgtgcactcc
     2221 gtcggtgacg atcacccggg cggcctgccg gaatctccgc ggacgcaggt gcggggcgac
     2281 cggctcgaaa cgccgggtcc ggaacggcgt gctcacggcc cgggcacctg cagcgtcacc
     2341 accggcagca ggccggcgtc gagcagttcg atcgtggtcg aacctggtgg tagggcgaag
     2401 ccgacctgcc cgctgacctg accggcatcg gtgagcgtgc tgccgccgag cggacggtcc
     2461 agcttgggac cgaacccagg ggtgacgccg acgccgtcgg cgctccgcgc acggaaactg
     2521 agcgactcga ccggaacgcg tccggcctgc tgggtgacgg tcacgtccag caccaggtag
     2581 cgcttgccct cgggcggggc gagttcgccg gcgctggtcc aggtggcacg ggtgatcgtg
     2641 acctcgccac tgccgtccgc ggtggtgaag gcggccgccc gggcgatgtc cggaccggtg
     2701 acgtcgacca cgtccgcggg ttgaggagtt gggctgaccg taaccagccg ggacggctcc
     2761 ccgctcgcgg gagccggggc gctcgccgtg ggcgcgatcg tgctcccccg gggcagcagc
     2821 ggcagcacgc tggccaaagc ggtcaggacg agcgcagccg ccaagccacc ggcgagtagc
     2881 cggacgtggg tgctgcgctt gcgctgcggt ccccggccgg tcggtggtgc gggcacgaag
     2941 ctggccggct cggacggcaa atgtccggcc ggtccggacg gcggcgccca gagtggctgc
     3001 gccgaagccg ccggcgtgcc tgttgcagga gcgaagctcg ggtggtcgga cgaaggcgtg
     3061 cccagaccct gggggccgtc cgcagagctc atcagccggt ccgatccggg cgctcaccgg
     3121 cgcgcagggc ggagccgacg atgccgaaga ccaccagcgg caacagccag caggccaatt
     3181 gggcgccgct ggcgaggctg tcgaacagca cgtccgcgtt ccaaccatcc gtcagcagcg
     3241 agaccccccc gaacgcagcg aggatcacga acgcaaccga gaacgcccgg cgcaccgact
     3301 cgcggcgata cgcgatccgg gccgagagcg tgaacgccag cgcaagcatg aacagcgaaa
     3361 gccatgcgaa cagtgcgccg accgcaaagg tgatcagcac agccggggtg atcgcggtga
     3421 aaatctggcc aatggtcacc gcgggcggag cggcgggggc ctgctgcggt ggcggcggcg
     3481 cgaaccactg ctgggttcct ggagcgggga acggtgccgg attcagttgg gcggtcgggt
     3541 ccaccatcgg ctgcaccggc aggtagccct tcaacgacgg acccggcggt ccgaacggct
     3601 cgtacggcga acgggtggcg gacggactgc cggcgtgatt cgggccgggc gcggtcacgg
     3661 ctgcggtgac cacttcgaag ggcacgttcg gattgcgccc gggggcaagg gacggcacca
     3721 gtgctgccaa ctcgggcgcg tccccggacg gcgggttgaa gtgcggctcg gcttcgggat
     3781 aggccggcgc tggcgccaag gcatggacgg gggccagggg ctcggcttcc gggatcaaga
     3841 aagcctgggg acgcacgcgt ggtgcgtact ccggtccgtc ctcccaggtg gccatgtcgc
     3901 aatcgtagac ggcggccgca gccgaccccc acgacgcttc ccggctcgcc gatcagcggg
     3961 cgtgaccggg ttcggtgtgc ctgcgctgca gcttccggtt aggctggttg ggtcgggccc
     4021 gatggcgggc cgaccacgat caggagttga ggaatcatgc cggttggcaa ggtgcgctac
     4081 tacgacgcgg agaagggttt cggcttcctg tccaaggacg agggcgggga cgacgtctac
     4141 gtgcgcgcgt ccgcgctccc agagggtgtg actagcctga agcgcgggca gaaggtcgag
     4201 ttcggggtgg tggacgggaa gaagggcgag caggccctgt cgctgcgcct gctggaggcg
     4261 ccgccgtccc tgtcgaaggc agcccggaag tcgccggagc agatggcgat catcatcgag
     4321 gacctgatca agatgctgga caacgttggc aatggctacc gccgcggccg tcatcccgac
     4381 gccaaggtcg ccggcaagac ggcgagcgtg ctgcgtgcgg tcgccgacga gttggagttg
     4441 tgaggatggc tgtggccctc gaactggacg ctgcctgcgc ggacgccgtc gaactggccc
     4501 gctcggctgc ggtgcggcgc gccggcgtcc tcgaggtcgg tgagcacgtc ggcgtgatcc
     4561 ccgaggacac gcgggtggcc acccatctct tcgagtgtgt gcacccgggc tacccgggtt
     4621 ggcagtggtc ggtcacggtg gcgcgcgccg cgcgggcccg ggtggtgacc gtctccgagg
     4681 tgacgctact tccaggtgag ggcgcgctgc tggctccggc ctgggtgccg tgggccgacc
     4741 ggatgggcgc gaaggacctc acccccggcg tgttggcgcc gacccctgcc gatgacttcc
     4801 ggctcgaacc gggctacacc ggcggcgagc atgcagccgt tgccgatcca gccgaggcca
     4861 gccagacccg cgcactggtc gccgaactgg ggctggggcg cgatcgcgtc ctctccctgg
     4921 ccggacgcga cgccgcggcc gagcgctggc tcgacgggcc cggcggtccg gacaccccga
     4981 tggcccagca ggctccggcg ctgtgcacca cctgcggctt cttcgttgcg ctgcagggtg
     5041 acctgggtcg cctgttcggc gcttgcacga acgaactctc gccgtccgac ggtgcggtgg
     5101 ttgcgcggac gcacggctgc ggcggtcact ccgacgtgcc cgagcccacc aagggtgtcg
     5161 acctatccct cccgatttgg gacaccatca gcgtcgacgt gagtctcttc gactgatcct
     5221 cgcagttcgt acctcactgc tcgtctgatc ctcgcagttc gtacctcact gctcgtcgag
     5281 tctcgtccct tcccagcgct ggcgcgctgt ggcggtcctc gtcaagactc gtcgcttccc
     5341 agcgctggcg cgctgtggcg ctcctcgtct gatcctcgca gttcgtccgg cgccgcatac
     5401 cggggcgtcg gtgaatcggc gcccagatcg tcgtccagtt cagcgcggtg tcgccgctgc
     5461 cgacggaaaa ggccgaaagt caggccgcaa aggccgagca caaagccggt gatcgacacc
     5521 ccgagatacc agcccttgcc ggccgcgttg agttccggca gcagccacca gcaggccagt
     5581 gcgccaatcg cgaacgctac cgttccggcg gtcaccacgg tcacgccgtc ggggtcgagg
     5641 gcgcggacag gcgcctgcac catcaacggc cgatggtggt ggctgttctg ggtcacggac
     5701 accacgatag gcccatccgt gtgagcccta ccatttgcgc catggcgaac cctggcactt
     5761 caacggcagc ccctgcccca aataccaaca aggccctgga ccaatggttc gagatcacca
     5821 aacgtggctc gaacctaggc cgagaggtcc gtggcggact ggtgacgttc ttcaccatgg
     5881 cctacatcat cgttctcaac ccgctgatca tcggcacgac tccggacaag gacggcaacc
     5941 tgatcaccgg gatgccgatc gcggacgcgg ccaacatccc gacttcgatc gcgatggtgg
     6001 cggctgcgac cgcgctgatc gccggcctga tgacgatcct gatgggcgtc tacggccgct
     6061 tcccgatcgc catcgccacc ggactgggcc tgaatgcgct gctggcctac accatcgcgc
     6121 cgaagatgac ctggccgcag gccatgggcc tggtggtctg ggaaggcatc ctgatcacca
     6181 tcctggtgct cgccggcttc cgcaaggcga tctttgatgc ggtgccgcgt tcgctgcgct
     6241 cggcgatctc ggtcggcatc ggcctgttca tcgcattcat cggtctggtg gacgcgggcg
     6301 tcgtccgtcc gggcgctggc accccggtgc agcttggggt caacaaccag ctcgtgggct
     6361 ggccgatcgg gatcttcctg atcgggctgc tgatcctgat cgcgctgttc gttcgcaagg
     6421 tcaagggcgc gatgctgatc gcaattctct ccgcaacggt gctggccgtg atcgtcgagg
     6481 cggtgctcag cctcggctcg aagagcgacg agaacgccac cggctggatg ctcaacgtcc
     6541 ccaagctgaa cgagttcgcc gcgccgaacc tcggcctgct cggccgggtg gacctgttcg
     6601 gcgccttcac cagtgcagcc ggagtcagcg cggtgatcgg cctgatcatg ctggtcttct
     6661 cgctgctgct cagcgacttc ttcgacacga tggggacgat cgtcgcggtc ggtgccgaag
     6721 gcgacatgct cgaagcggac ggtaacccgc cgcacaccca ggagatcctg ctggtcgact
     6781 cggtgtccgc catggccggc ggtttcggct cggtctcgtc gaacacttcc tacatcgaat
     6841 cggccgcagg tgtcggtgaa ggtgcgcgga cgggtttcgc gtccgtggtt accggcgcgg
     6901 cgttcctgct cagcctggtg ctcgcaccgc tggtgaacat ggtgccgtcc gaggcagcgg
     6961 ccccggcgct gttcttcgtc ggcttcctga tgatggcgca ggtcgtgcac gtgaattggg
     7021 aggacccgga agagggcatt cccgcgttcc tgaccatggt gctgatgccg ttcacgtact
     7081 cgatcaccgt gggtatcggc gccggtttca ttgcctacgc gctgatcaag atcatccgtg
     7141 gcaaggccaa ggaagtgcac acgctgctct acgtggtcgc ggccgcgttc gtgctctact
     7201 tcggacaggg cgtgatcacc gcgctgatcg gcggctgagc ttgccgtcgt cccggaacgc
     7261 actccacgtc gttccgggac accacgtcat cccggcgcag gccgggatct ctgaccgtct
     7321 tggagatccc ggccttcgcc gggatgacgt ttggtgcggg atgacgtttg gcgtaggccg
     7381 ggatctcagg ctgtcactcc tggcggaggg cggcgatcac gttcaggcgg gacgcggacc
     7441 gggccgggcc gatggacgcc aggacgccga acaccagccc gaccaatacg gtcgccagca
     7501 ggccggtcca ggggaatgcg tagggcatct gccagcccac cgcgcccatc gcggtaacca
     7561 gggagtagcc cagccacaag ccggcgatcg cgccgaagcc ggtgccgatc acggccagca
     7621 gtagtgactc ggccagcacc atccggcgta cctgccgccg ggtcgccccg accgcccgca
     7681 gcatgccgat ctcgcgggtg cgggccagga cgctgatcgc cagcgtgttc accagcgcca
     7741 gtagggacgg cagcgccagc gcggccacca gcacgtcgaa gatgatgatc gtctggtcga
     7801 acgtccgcat ctgctctgcg cgccacgcct ccgattcgta caaccggaac gccgggtagt
     7861 cgccgaccac cttcgccagc cgggcctggg tctgcgtcgg gtcagcggac ggcgtccggt
     7921 tggccatgat cagcaggtcc gcggtcacgt tgaaatcgcg ttcgagcaac tcctgtgagg
     7981 tgtacagcgt ggacagcttg gcgttcaggt agtcgttgcc gatcccggcc acatggtagg
     8041 tgcgagcgcc attgggagtg tccagctgga tctgctgccc gagcgcgagg ttgtgctgcg
     8101 cggcgtagat gccgttcgcg atcagccagc gcccgctgcg caactggtcc acggcggcgt
     8161 ccgacgaccc cgagttccag tcgaaactgg ccaccttgag atagttttcc ggatcgatac
     8221 cgagtacctg gacgtccccg ccggcagcct tggcctgggc cacccgcaag gaggatgcgg
     8281 ccgcgatccc gtccgtcccc gcgacctggg cggccaagcg tggaccggcg gccacattgc
     8341 cctggccgag gatgatcgat tgcggcatca ccaggtagtc ggccgagagt gacttgtcca
     8401 gataagtggt gaagccggcg aagatcgaag tcaccacgct gatcatcgcc acgatggacg
     8461 ccagcccgag catcaccgcg gacaccgtca ccgcgctccg gccagggttg cgctgcaggt
     8521 tgctgcgcgc gatcgcgccc tcgcaggcgt tgaagctctc caggggagcg ctcacccagt
     8581 tcgcgatcgg gttcaccacc gccggtgcga ccagcgcgat acccaccagg aagaccaccg
     8641 atccgaaccc gaccagaccg gccgaaccgg aggtaagccc gaacagggac gcgaccagca
     8701 gcgcggcgcc gatccaggcg cgccggccaa cccggcgttc gaacacctcg cccaactgcg
     8761 gacgcatcgc ctccatcggc gtcacccggc cggctgcgcg ggccggtacg acggcggcca
     8821 ggatggtcac gcctacaccc agcacgatcg cctcggccca ggtcgccgcg gtgaacaatg
     8881 gtccgccgat ggtgaaatgc atcaccgact tgaagatcgg tgccattgcc gcaaacagcc
     8941 cggcggccat cgcccagccg gcggcgatgc ccagcgccgt gccgactact ccttggacga
     9001 gggactcgat caggaagagc ctcgaggcgc ttcgcctggt cattccgatc gcccgcagca
     9061 tgccgatgtc gcgtcggcgc tcggccacca ccgtccggaa gctgttcagg atgatgaacc
     9121 ctgccgtggc gagggcgaag atgccgaaca tgttgaacgc gaactgcgcg acctgcagcg
     9181 aggccagcag cgacgactcg gtggagagcc cgcccacctg gtagtccggt ccgagcgcgg
     9241 cactgaccgc agcttcggta gcggcgcggt cggcgtccgg ggtgaacacg gcctcgatct
     9301 gggtcagctg gtcaccgagg cccagcaact gctgggccgc ggcgagggtg acgaacacct
     9361 gctcctggcc gggcagggtg gccgtgctga gcagcccgac gacctggaac cgggcggtcc
     9421 ccccggcggc cggcagcacc agttggtcgc cgagcccgag gcccagctcg gcggccaggt
     9481 cggtattcag cacgaccgcg gtgcgatcgg tgggcgccag cacgcgtccg gacgccagcg
     9541 ggaagtcgcg gacggcggtc gcggtgggta cgtccacccc gaccagtaca acctgcgcga
     9601 gggcgtccgc ggcggcgtcc gcgctgcggg ggagtgggac ggtgcgctgc acgcccgggc
     9661 ttgcggtggc gacctgcggc acggtcaaca gccggtcggt gacgtcggcc cggaagggct
     9721 ggttgtacgc gctggtgacg gtgaggtcga ccttgccggc agccgaaagc agattgtgcg
     9781 tgaaggcctc caccatcgcc ggcagaatgc cgttgaggcc gaaggtcagc atcaccccga
     9841 gcaccaccgc gagcgtggtc agcagcgagc gcagcccgcg gccgcgcagg tagcgccagg
     9901 caagggtgag attgaggttc acagcaggcc ggccctttcc agtgcgagcc ttggcccctc
     9961 gggagcgtcg tcgagatcga ggtcgtcgat gatccggccg tcccgcatca gcaccaagcg
    10021 ctcggcgaac ccggcgatcc gcgggtcgtg cgtgaccatc aggacgctgc ggccgtaggc
    10081 ggcgctgatc tcgcgcaaca gctcgccgat ctcggtggct gagcgtgagt ccaggttgcc
    10141 ggtgggttcg tcggccagga tcagcgccgg ttcggtggac aacgccctgg cgatcgcgac
    10201 ccgctgctgc tgcccgccgg agagctggtc tggccggctc tgcagccgat cgccgagccc
    10261 cacccgttca agccaggtgg tggccctgga ctcggcgtcg cggacgccgt ccagccgcag
    10321 cggcagggac gcgttctcga ccgcgttcag caccgggatc aggttgaaga actggaagac
    10381 gaagccgatc cggcggcgac gcagctcggt gaggtcgtcg tcgggaagat cgccgagctg
    10441 ggcgccgtcg atgctgatcg tgccggacgt gggggcgtcg agcccgccga gcaggttcag
    10501 cagcgtcgac ttgccgcaac cggacggccc catcacggtc acgaactcac cggcgccaac
    10561 gctcaggtcg accgcgtcca gcgcggtgac ctgggctgcg ccggtgccgt agatcttggt
    10621 gagtccggcg atctgcacgg tcggcggacg gtcggacgtg gtcacttcgt gcgcacctgc
    10681 tggccgtcgc tcagggccgc cgcgccggtg gtgaccacgc ggtcgccttc cgacaagccg
    10741 gagatgatct gcacggacgt gtcggtgctc agcccgacct cgacctcgcg agcgtgggcg
    10801 accgagtcgg cgtcgaccac gaagaccgtc cggccggtgc cgcgggtctg cacggcggcg
    10861 accgggacaa ccagcgcgtc cgggatcgac tcggtggcca gttcgaccga gccgctcatg
    10921 ccggacaggg cgccggagtc gctggactcg atcgccacgt cgacccggaa ggcgaccgtg
    10981 ccggtcgagg tggtctcgga ttggctgtgc acccgggtta cctttcccgt gaacggctgg
    11041 tcgaaggcgt ccaggctgac cgtggcgtcc tgatcggtgg tgatgccggc gatgtcggtc
    11101 tcgttcacca tcgcctggaa ttcgagcctg gtcgggtcga ccagtcggaa gaccgggacg
    11161 cccggagtcg cgcccgcgcc cttctccacc gtgccgacga aactgacgac gccagcgaag
    11221 ggtgcggtca gttcagcgtg gtcgaggttg gcctgggcca ggtccagggc gcggtcagcc
    11281 gctttgcggg catcgttggc tgcggttcgc gccagcccaa cccgtgcggc gaccgacagc
    11341 tggtgcaggc ccgattcggc gcccttgagc gctgcgttgg cggtgctctt ggccgtcttc
    11401 aggctgcgca gggttgggcg catcttggcc cgctcagcgt tgctcgcgtc ccggtagtcg
    11461 gcgaggtagg cagagtagtt cttcgctgcg gtggacacct gagagcgggc tgcggacaac
    11521 gccgcattcg cggccgaacg gtcgatcgct gccgggacgc cacggttaac cgcgtccagt
    11581 tgggcttcgg ctgccgaggc ggcggcccgg gcctgggcga cggccagttt cagcggtccg
    11641 gtggcgaggg tggcgaggac ttcgcccttc tcgaccgtgt ccccgtccgc caccgtcacc
    11701 ttggcgatgg tggcagcggc ggacgggtag acgccggcgg tgtgcgcggg cgcgagggtg
    11761 ccggacgcgc tgacggtggc cgtcagctcg gcgtgttcgg caatcgcggt ctcgacctcg
    11821 ggacggctgt tggtcaccga gtaggcgtag ccggcgccga gggctagcac ggctgcggcc
    11881 gcgatgccgg tggtgaccag ggtcttgcgg ttcatgctgg ggtcctaacc ggatgcgagt
    11941 agagctgttc gatggcattg acgagttcgg gtgggatcgg gacggcgtag ctgccgtccg
    12001 ggacgccgag gatgcgctcc tgggcttggg agatggctgc gccgccggcg gtcatgacct
    12061 gcacgaaggc ggtcgcgtcc ggggattgaa gtgcgcggcg ccagagttgg tcggcggcgt
    12121 cgaaccagag cagcaggatc aggtcggtgg ccgcgcctgc gtccgcgacg tggaaggagc
    12181 cgtcggccac cccggcggcg atcaccggtt ccagcacggc gcgagcgcgc tcccgccagg
    12241 cgtcgaacag ccgatgccgg agcgcgaagt tctccgctcg ataaaggaat gccgcgtagc
    12301 cgaggtcggc ctgcccggac ttgtaggcgg cggacgcggt catcagccgg ccgagccggt
    12361 gcgcgccgtc cccggccacg ccgtcgatcg cttcggtgag gtggttgaaa agcgactcgc
    12421 cgaagcgtcc gaccaactgc tcctgcaggt cggctttcga gccgaagtag tggtagaagg
    12481 tgcccttcgc cacgcccgcg gtctgggtga cctgttcgac gctcatcgcc tcgaaaccat
    12541 ggctgcggca aagctcgaat gcgacgtcga gcagttcttc gcgccgcacc agcggatgct
    12601 tggtgattcg agccatccgt cctccgttcg ttccctgggc ctggtcaccc gtcttcgcag
    12661 agtggaccga agctcgccgt cctggcgcga aaccgtccac cttggctgag ggctggcttc
    12721 actgaccgac cgtcggtcag tctactcgtt ggttgatgct ttgcggcctg aaagggcgcg
    12781 gtcaggagcg aagaatcgcg accagatcgg gtggcatccg gccagccgcg gacccctgcc
    12841 gcgtccactc ggggaaggcg tccagccagc cgaggaggcc gcgcacgaac gcccggtgct
    12901 cgggttccag ctcggccaca atcggacgcc agttggccgc cagcgcggat gcggccgcat
    12961 tcggcacata gccggccgcg acgccaggag cgggccgcag cggcaggccc aggtcattgc
    13021 gcaccaggtc gccaagcgac catttcgtgg ggacgccgac gcgggcgagg tcgaaatcca
    13081 gtgcggtgat cgcatgcgcg tcgaactctg cggagtgctc gccccaggct tcagccgcca
    13141 gccaggtccc gagcaacgcc caggccgcag tcagtcgctc cgggggcaca tgtctgcccg
    13201 cgagcatcag gtcgagcacg gtccggccgg gcgtgtccgg gcggatgatc ggtgcaccgg
    13261 gctggcgaag gaacaccggt cccagcttgc ccaacaggcc ctggctgggg acgctcgcgg
    13321 tcgtagtggt gcgagcggcg atcgcccgga gccggtcggc cagttcgctg ggcgccccga
    13381 agaattcgcg aacctcggtg atcgcgatcg cgaagaggcg caccgacccc atgagtccag
    13441 ggtaatccag gacgcatcac cggttgccgg ttgcgtccct gccgccggcg gtcccgggcg
    13501 cgtcggcggg catagattgg ggtgcgtgaa cccgcggtcc cgacgcctga tcgtcaccgg
    13561 cgcactgttg gcgctgatcg ccctggtgat gctgggaacg ctgctcggtc tgggccggta
    13621 ggctccgcct catgatcgag ggggcacgcc gcattctggt gagcatggcc gcggcgttcc
    13681 tgctgacgct ctgcgccggg gttcaatccg catttgccga gacgccgacg ccaacaccgg
    13741 ttgcgacagc tgcggcgacg actggctcgg acacgaccgc ggacgctccc gacgtcgcgc
    13801 ccgacaactc ggcagtggtc tgggcgctgg gcgctgccgg agtattggcc gtcattgccg
    13861 gcgcggtggt cttcctgcgc cgcaactgaa gcggataggc ccggggtgcc ggcgaaacgg
    13921 gacaattcgc gctccgcgtg gcccgaactt gccggtaaac gaaatcaacg cgccactagg
    13981 ggcaggtttg tcccgttcgg gttcggggaa gccgccagcg gctcaggcca gttcgttgag
    14041 gtcgatcggc acccgccgaa acagcgctcc gaactcggcc agcacccgtg cgttggggtc
    14101 cggcacggac aacggtccca gccgtcgctc aacctgcgcc aggcgcgcca gctccgggag
    14161 cgccaggacg ctggccaccg cggccccgtc cccgcccaga gccagggcgt caagacgtcc
    14221 ggcctcgcca accagtaggt cggccgcgtc ccgtccggcg gcctcgaagg aacgctcggc
    14281 ctggttcgcc cggcgccgcg cgtagcgctg ctgcgaccag ccgccggcct tggtgcgtcc
    14341 ctgcacgtaa tggctgccac ggcgtccggc caccaactcg ctgccgacga agataccgac
    14401 cgcgtgcgcc ttgcgccgca cgatcagcgc acccacccgg cgcggacgcg cgaacgccgc
    14461 gaccacctca gccagcgggt cgccttccgg caggggcccc cagagcagtc ggacgcgagc
    14521 cgcagccccg tccgcagcct gcaggacgag cgcgtcggta tctacttcga ccgcaggcgt
    14581 gccatgccgt tccgcgaaac cggccagcca cccggcgagc cgatccggcg gcacagtcac
    14641 ggtacggggc cacgtggtcg agctcatggc tatagtctga cctcaatcgc agaggaggat
    14701 gatgagcgac ctcatcgaca ccaccgagat gtacctgcga accatctacg agctcgggga
    14761 agaaggtgtg ccgccgctgc gtgcgcggat cgccgaacga ctcaagcaga gtgggccgac
    14821 cgtctcccag acggtggctc ggatggaacg cgacgggctg gtctcggtgc acccggatcg
    14881 gcacctcgtg ctgtccgagc acgggctgga actggccatg cgggtgatgc gccggcatcg
    14941 gctggccgag cgcctgctga tcgatgtgat cgggctggaa tggtcgctgg tccacgagga
    15001 ggcctgccgc tgggagcacg tgatctccac cgacgtcgaa cggcggctgg tggcgatcct
    15061 cgatcacccg gtgatgtcgc cgtacggaaa cccgattccc gcgctcgagg aactggacgt
    15121 cgatccggcg ccggagtcgt tcctggacgg caccgagctg ttgtccttca ggctggcgga
    15181 cggtcaggcg cacgaggtcg tgttgcggcg gatgagcgag tcgatccagt ccgatccgaa
    15241 gctgctggcc gcgctggctg aggccggctg cgtgccgggc aggccggtac gcgcgtccat
    15301 tctcggcagg aacctgctgt tgggcagcca gaacgaggtg atcgtgccgg ctacggtggg
    15361 cgaccacctg ttcgtagcgc cggagtagcc ggaggagacg gagcaggcgg ggctggagcc
    15421 cagcggacgg gaatagccga cgcgaccggg acgttctgaa ccacgtaccc gatcgtgagg
    15481 attgtccatg gcaaccattg ccctgaccga agccgagttc ggcaagacca tcaccggaaa
    15541 tggcatcgtt ttcgtcgatt tctgggccgc ctggtgcggc ccgtgccgga tgtttggccc
    15601 cgtgttcgag gcagcgtccg aggcgcatcc ggacgccgtg ttcgccaagg tcgacaccga
    15661 tgaccagcgc gggctcgccg gcgacctgga catcagctcc atcccgacgc tgatggcctt
    15721 ccgcgacggc tacctcgttt accgcgaggc cggggcgctg aacaagagcc agttcgagca
    15781 cctcgtccag caggtgaagg cgctcgacat ggagaagctc aaggccgaag caggcctggg
    15841 ttcagccgcc gatgccggac gcgatgatgc cggtgacgat cactgaggtg atggtgaccg
    15901 tggagaagcc ggcgacgatc atcttcggca ggatcgcgcc ctcgat
//
LOCUS       DAOPRQ010000060        15277 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_3280_length_15277_cov_11.706018, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000060 DAOPRQ010000000
VERSION     DAOPRQ010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 15277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15277
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3280_length_15277_cov_11.706018"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            223..1230
                     /gene="gap"
                     /locus_tag="PKJ61_08685"
     CDS             223..1230
                     /gene="gap"
                     /locus_tag="PKJ61_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOC13938.1"
                     /translation="MTVKVGINGFGRIGRNYFRALVASGADLDVVAVNDLTDNKTLAH
                     LLKYDSILGRFPGEVSYDDDALWVNGKEIKAFAERDPSALPWGELGVDIVIESTGFFT
                     DATKAKAHIDAGAKKVLISAPAKNEDITIVMGVNDDKYDAAVHNIISNASCTTNCLAP
                     MAKALNDGLGIVKGLMTTIHAYTQDQNLQDGPHSDLRRSRAAALNMVPTTTGAAKAVS
                     LVLPELKGKLDGYAMRVPTPTGSATDLTFEAARETTVEEVNAIVKAAAENGPMKGFLV
                     YTEDEIVSKDIETDPASCVFDAKLTKVIGNQVKVLGWYDNEWGYSNRLVDLTALVGSK
                     L"
     gene            1317..2525
                     /locus_tag="PKJ61_08690"
     CDS             1317..2525
                     /locus_tag="PKJ61_08690"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002534979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOC13939.1"
                     /translation="MKSVSDLGDLRGKRVLIRCDFNVPLDGDTITDDGRIKAALPTLT
                     QLVEAGARVVVMAHLGRPKGKVNPEFSLAPVAKRLGELIEAPVVLAADVVGDSAAATV
                     AGLGEGEIALLENVRYEPAEESKVDAERAELAAKYAALADVFVSDGFGVVHRKQASVY
                     DVAKLLPSAAGALVKKEVEVLKRLTEAPERPYVVVLGGAKVADKLGVIDNLLKVADTL
                     VIGGGMAYTFLKALGHEVGISLLDAEKVDVCADYLKQAEAAGKQILLPVDVRVVAELD
                     IPARTSSKVTIVAADAIPADQEGADIGPETEAIFAEAITGAATIFWNGPMGVAEIPEL
                     ASGTEAVAKALAASSGFSVVGGGDSAAAVRNLGFADDQFGHISTGGGASLEYLEGKVL
                     PGLAVLDEEN"
     gene            2528..3310
                     /gene="tpiA"
                     /locus_tag="PKJ61_08695"
     CDS             2528..3310
                     /gene="tpiA"
                     /locus_tag="PKJ61_08695"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007077852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOC13940.1"
                     /translation="MARKPLMAGNWKMNLNHVEATGLVQKLAWTLTDAKYDPERSEAA
                     VIVPFTDIRTVQTLVEGDKLPLKFGAQDVSVHASGAYTGEISAEMLAKLNCAYVVVGH
                     SERREYHGETDELIGEKAKVVFAAGMTPIVCCGEVLDIRKEGTHVPFVLGQVEACLDG
                     LTAEQVAAMVIAYEPVWAIGTGEVATPEDAQEVCGAIRGYVAETFGVEAGEAVRIQYG
                     GSVKANNIVEIMAKPDVDGALVGGASLNADEFAKIVMFYSVI"
     gene            3495..3758
                     /gene="secG"
                     /locus_tag="PKJ61_08700"
     CDS             3495..3758
                     /gene="secG"
                     /locus_tag="PKJ61_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HOC13941.1"
                     /translation="MTWPIVTLSVVLILTSIVLALFILLHKSRGGGMSDLFGGGMTTG
                     MGGTSVAERNLDRLTVIVGLVWLASIIGLLVLYRFFPEIGTLG"
     gene            3789..4145
                     /locus_tag="PKJ61_08705"
     CDS             3789..4145
                     /locus_tag="PKJ61_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007433696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein RbpA"
                     /protein_id="HOC13942.1"
                     /translation="MAGGSAIRGSRVGAGPMGEAERGDAAPRLYVSFFCANRHETRPA
                     FAADAVVPETWDCPRCGLPANLDSENPPPPPKIQPYKTHLAYVKERRTDAEAVAILAE
                     ALDALRARRAAGEVIY"
     gene            complement(4185..4922)
                     /gene="pgl"
                     /locus_tag="PKJ61_08710"
     CDS             complement(4185..4922)
                     /gene="pgl"
                     /locus_tag="PKJ61_08710"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015070951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HOC13943.1"
                     /translation="MTGRQPQVVRHRSHEELADTTARRLLATLTQLQRDGGVAHLCLT
                     GGRIATAMYARLGELLADSEVDSSRLELWWGDERFLPTEDPERNAGPTLALLARYFQV
                     DPARTHPMPSADGVVDAGASANTYAKELGETRFDICLLGMGPDGHVASIFPNHPSSEP
                     TTQPVIGVSDAPKPPSERISLTLATLSRSRQVWFLVSGEEKGDSLARALAGDPELPAA
                     HVEGTDCTLWLIDEDAAVKLPYFDCWA"
     gene            complement(4919..5851)
                     /locus_tag="PKJ61_08715"
     CDS             complement(4919..5851)
                     /locus_tag="PKJ61_08715"
                     /inference="COORDINATES: protein motif:HMM:NF021632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase assembly
                     protein OpcA"
                     /protein_id="HOC13944.1"
                     /translation="MIVQLTDTNAAALQEQLGQLRHRLGGISGVVFTLVVVVDQGDYD
                     RAMAAVVDTALEHPSRILLVTNGRARADRLDASIRAGEDAPGEIVTLKFHGELRKHRD
                     SVVLPLLLPDLPVIVWWSGCSPVAPASDQLGRLGTRRITDAMGDPDPLAALEVRARNY
                     CPGDTDLTWTRLTRWRGLLAAALDAYPMNVTRATVGAAPDNAAASLLAAWLGDRLNVG
                     VAVTRHPGVGITEVTLGAETDEIRVARTDGNLAVISAPGLPERLVALPRRDLNTLLTE
                     ELRRMDDDPIYAEAMAALVRRLDRATPQTGGARK"
     gene            complement(5871..7343)
                     /gene="zwf"
                     /locus_tag="PKJ61_08720"
     CDS             complement(5871..7343)
                     /gene="zwf"
                     /locus_tag="PKJ61_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HOC13945.1"
                     /translation="MAGPSALVLFGVTGDLARKKLLPAIYDLANRGMLPPGFGLVGFA
                     RRDWAAEHFADVVADAVRSHARTPFREDVWRQLVAGVRFVSGELDDDAAFEELKRTLA
                     ELDETQGTGGNHAFYLSVPPTAFDITVAQLRRHGLADRPRGSWRRVVIEKPFGHDLAS
                     AQELERKLAKAFEPTSVFRIDHYLGKETVQNLLAFRFANTMFEPIWNRNYIDHVQITM
                     AEDIGIEGRAGYYDGIGAARDVIQNHLLQLLALTAMEEPTSFGADELSAEKRKVLAAA
                     STPADLGAHTARGQYAAGWAGGRLVAGYLEEKGIPADSHTDTFAAIRVDIDNRRWAGV
                     PFYLRAGKRLPRRVTEVALNLRTAPHLPFDHADVRSLGNNALVLRIQPEEGITLRFGA
                     KVPATSELREVNMEFGYDTSFVESSPEAYERLLLDVLRGAEPLFPQPAEVELSWRLLD
                     PVLDHWASQEEPPQAYPSGTWGPTSAVEMLARDGFSWRRP"
     gene            7487..8419
                     /locus_tag="PKJ61_08725"
     CDS             7487..8419
                     /locus_tag="PKJ61_08725"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HOC13946.1"
                     /translation="MRPAEEGAPVSVLAPVDWRATAKAYLALAKPRIIELLLVTTVPA
                     MFLAAGGFPPWQLVLWTLLGGTAAAASANVFNSVLDADIDARMRRTRRRPMALDLVTP
                     ARAIAFGAVLGVASVLILGLGANWLSAGLALAANLYYVFVYTMALKRRTSQNIVWGGV
                     AGCFPPLIGWTAVTGSVAWAPLVLFAIVFAWTPPHTWALALRYREDYLNAHVPMLPVV
                     APPVTVAWRILAYSALTMAVSLLLWPVAGTGWGYPLVALVAGLAMLAEAVALLVRAKR
                     GLTDAALKPMRLFHLSNSYLAIVFIAAAVDPLLF"
     gene            complement(8594..9496)
                     /locus_tag="PKJ61_08730"
     CDS             complement(8594..9496)
                     /locus_tag="PKJ61_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007077842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HOC13947.1"
                     /translation="MDLVGSPAALRGWSIASLVANMGLVVTGGLVRVTGSGLGCSTWP
                     QCEPGSYVPHGDAPLHTYIEFGNRLLTFVLVAVAIGTLIAAWRARDAAGNPVRRVRVL
                     ALLAAAGIPAQAVIGGISVLARLEWWVVGLHLVPSLALIMVCTVLVHEVFGAAPAGAT
                     AAARNLAWVVLALGWLVLLLGIIVTGAGPNAGDADVHRSGFDLTAVARTHSLSVWAAT
                     IAIVALLWLARRTPALFRATLLLLGVVVLQGAIGYAQYFLGVPPMLVALHMLGTALFC
                     AAIANVWWLARVPARNPSLMAYSG"
     gene            complement(9487..10242)
                     /locus_tag="PKJ61_08735"
     CDS             complement(9487..10242)
                     /locus_tag="PKJ61_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOC13948.1"
                     /translation="MNLDLGPGAAPATRPRRVIAHSLMETRLIVRNGEQLLLALVIPL
                     GLIVAQAVLGPRFGIAREPFVASVIALGLWSSGFTSPAITTAFERRYGVLERLVATPL
                     RRADLLTGKATMIAILAVGQAAVLALAGLIVGWRPAPTLLQSGVTLLVVLLGLVAFAS
                     LALCLAGTVSAELTLGLANLVYLGGAALGILVPVASFPAWAQPLVASLPTTAVAESLR
                     VWAAGGVALWPLLVVLIWAVAGLALARKVFRWI"
     gene            complement(10239..10946)
                     /locus_tag="PKJ61_08740"
     CDS             complement(10239..10946)
                     /locus_tag="PKJ61_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOC13949.1"
                     /translation="MSAALTLTDVRIQLGRRPVLTGLSLSAAVGEVTAVLGPNGAGKT
                     TMIRCCTGLLSPDAGTIDVLGRAAGAPGANDRVGLMPQATGAWSGVRPLELLRYLAAL
                     YASPLPVAELVEMLGIGTFAQTPYRRLSGGQQQLVNLAGAIIGRPELVFLDEPTAGLD
                     PHVRRTVWQLIRDLRAAGVAVVLTTHAMDEAEKLADRVILLNAGRAVASGTVGELTAD
                     GNLEQLFLSLTTPGGAA"
     gene            complement(11062..13620)
                     /locus_tag="PKJ61_08745"
     CDS             complement(11062..13620)
                     /locus_tag="PKJ61_08745"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014848021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HOC13950.1"
                     /translation="MRSGLFVVFGLLGLSLAACSGTGAEPTHTTHKSEFDLPLPDLHA
                     VTFLGGVSGWYLLLIGMLICLAGFGFGIYSYMKLKNLPVHQSMREVSELIYETCKAYL
                     LQQGKFLLTLFAFIGAVIFVYFGFLNTTGGWGRVIIVLLFAIIGMAGSYGIAWYGIRL
                     NTFANSRTSFAALKGKPFNVYDIPMRSGMSVGMVLISTELIMMLAIMMFLPAEIATAC
                     FLGFAIGESLGASALRIAGGIFTKIADIGSDLMKIVFKIGEDDPRNPGTIADCTGDNA
                     GDSVGPSADGFETYGVTGVALITFILLGVQADLQAHFLVWIFFIRAIMLVASALSYFG
                     NALWAKSKYGNAEEMEFETPLTSLVWITSIVSIVLTFIISAMLIPNVGGNTNLWWQLS
                     LIISFGTLAGAIIPELVKVFTGVKAKHVEEVVKSAKEGGPSLDILSGLVAGNFSAYWL
                     GIAIVGLMGGAYGVSTMIPAIDPANAAAGGIMLAPGVFALGLVAFGFLGMGPVTIAVD
                     SYGPVTDNAQSVYELSQIEQIPGIEDELQRDYGITPNFERAKYLLEANDGAGNTFKAT
                     AKPVLIGTAAVGATTMVFSIIMGLTNGLQTDLLANLSLLHVPFLLGLITGGAIIYWFT
                     GASMQAVTTGAFRAVEFIKGNINLETSEKASVKDSRRVVEICTQYAQKGMFNIFLGIF
                     FATLAFAFVEPYFFIGYLISIAVFGLYQAIFMANAGGAWDNAKKIVEVDLQAKGTELH
                     DASIVGDTVGDPFKDTSSVALNPVIKFTTLFGMLAIELSLIINNSAINAVLAVVFFLA
                     SAYMVWRSFYGMRIDQPMLTAADFAYLKDAPSPELSTGAPAPAAAEHVADAAKV"
     gene            13873..14595
                     /locus_tag="PKJ61_08750"
     CDS             13873..14595
                     /locus_tag="PKJ61_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HOC13951.1"
                     /translation="MKSETEPNGDAAVAEAEQQAADASTRSRVARSILQHGPSTANDL
                     ASRLGLTPAAVRRHLTVLASTGQVTSREQRVYGHRGRGRPAKVFALTDSGRGEFYQAY
                     DALAINALDHLAEVAGPEAVEAFSAKVAEAVAARFAIIAPEYESAADALVEALTAEGF
                     VASLQPVASGVQLCQYHCPVAHVAAEYPQLCEAETRVFARLLDSHVQRLATIAHGDGV
                     CTTHVPRPVVAAMYPSPNRRAN"
     gene            14597..>15277
                     /locus_tag="PKJ61_08755"
     CDS             14597..>15277
                     /locus_tag="PKJ61_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HOC13952.1"
                     /translation="MTSTTTAPGLGSTQEEHLEALGTYQYGWHDSDLAGTSAKRGLNE
                     DVVRNISALKSEPEWMLKLRLKALKLFDRKPMPAWGADLNEIDFDNIKYFVRSTEKQA
                     QTWEDLPDDIKRTYDRLGIPEAEKMRLVAGVAAQYESEVVYHKINEELERQGVIFLDT
                     DTGLKQHPEIFEEYFGSVIPVGDNKFASLNTAVWSGGSFVYVPKGVHVTIPLQAYFRI
                     NTENMGQFE"
BASE COUNT         2639 a         5062 c         5203 g         2373 t
ORIGIN      
        1 tcgacggaca gcgcgccagc gctgtccgga atagacgacg acggataacc ggcgtcagct
       61 ggacccggaa ctagggtcac tggatcgagc cggatctgca cgttgtttcg gatcctcgac
      121 agcggcaacc gacgctcctt cgaaccgcta gggtggacgt tgtaagccgg ctggtcaagc
      181 gctggccgat gcatatcagt cgaagaagga gatcctctaa acatgaccgt caaggttggc
      241 atcaacggct tcggccggat cggccgtaac tacttccgcg ccctggtggc atctggcgcc
      301 gatctcgatg tggtggccgt caacgacctc accgacaaca agaccctcgc gcacctgctg
      361 aagtacgact cgatcctggg ccgcttcccc ggtgaggtct cctacgacga cgacgccctc
      421 tgggtgaacg gcaaggagat caaggctttc gctgagcgcg atccgtccgc gctgccgtgg
      481 ggcgagctgg gcgtcgacat cgtgatcgag tccaccggct tcttcaccga cgcgaccaag
      541 gccaaggccc acatcgacgc cggcgccaag aaggtcctga tctccgctcc ggcgaagaac
      601 gaggacatca ccatcgtgat gggcgtgaac gacgacaagt acgacgccgc cgtgcacaac
      661 atcatctcga acgcgtcctg caccacgaac tgcctggccc cgatggccaa ggcgctgaac
      721 gacggtctgg gcatcgtcaa gggtctgatg accacgatcc acgcctacac ccaggaccag
      781 aacctgcagg acggcccgca cagcgacctg cgtcgctccc gcgccgccgc cctgaacatg
      841 gtcccgacca ccaccggtgc ggccaaggcc gtctcgctgg tgctgcccga gctcaagggc
      901 aagctggacg gctacgcgat gcgcgtcccg accccgaccg gttcggccac cgacctcact
      961 ttcgaggccg cccgtgagac caccgtcgag gaggtcaacg cgatcgtcaa ggctgctgca
     1021 gagaacggcc cgatgaaggg tttcctggtc tacaccgagg acgagatcgt gtccaaggac
     1081 atcgagaccg accccgcgtc ctgcgtgttc gacgccaagc tcaccaaggt gatcggcaac
     1141 caggtcaagg tgcttggctg gtacgacaac gagtggggct actccaaccg cctggtcgac
     1201 ctgaccgcgc tggtcggctc gaagctctga tctgaccaaa cagctgtgca acgggccggc
     1261 tcgcgtcatc agacgcgggt cggcccgttg cttagtaacc gacagaaagg ctagacgtga
     1321 agtctgtatc cgacctcggt gacctgaggg gcaagcgggt tctgatccgc tgcgatttca
     1381 acgtgccgct cgatggcgac accatcaccg acgatggccg catcaaggca gcgctgccca
     1441 cccttaccca gctggtcgag gccggcgcca gggtcgtggt catggctcat cttggccgcc
     1501 ccaagggcaa ggtcaaccct gaattctcgc tcgccccggt agccaagcgg ctgggcgagc
     1561 tgatcgaggc accggtcgtg ctcgcggccg acgtggtcgg tgactcggcc gcagccacgg
     1621 tagccgggct cggcgaaggc gagatcgcac tgctggagaa cgtccgctac gagccggccg
     1681 aggagtccaa ggtggacgcc gagcgcgccg agctggccgc aaagtacgcg gctctcgccg
     1741 acgtcttcgt ctccgacggc ttcggcgtgg tgcaccgcaa gcaggcgtcc gtctacgacg
     1801 tggcgaagct gctgccgagc gctgccggcg cgctggtgaa gaaggaagtc gaagtcctca
     1861 agcgcctgac cgaggctccc gagcggccct acgtggtggt gctaggcggc gcgaaggtgg
     1921 ccgacaagct cggcgtcatc gacaacctgc tcaaggtggc cgacacgctg gtgatcggcg
     1981 gcggcatggc ctacaccttc ctgaaggcct tgggccacga agtgggcatc tcgctgctgg
     2041 acgccgagaa ggtcgacgtc tgcgccgact acctcaagca ggccgaggct gccggcaagc
     2101 agatcctgct gccggtcgac gtccgcgtgg tagcagagct ggacattccg gctcgcacgt
     2161 ccagcaaggt caccatcgta gccgcggacg cgatcccggc cgatcaggag ggcgccgaca
     2221 tcggccccga gaccgaggcc atcttcgccg aggccatcac cggcgcggcg acgatcttct
     2281 ggaacggtcc gatgggcgtc gcggagattc ccgagcttgc cagcggcacc gaggcggtgg
     2341 cgaaggccct cgcggcctcg tccggcttct cggtggtggg cggcggcgac tctgcggctg
     2401 cggtgcggaa cctcggcttc gccgacgacc agttcgggca catctcgacc ggcggcggcg
     2461 catcgcttga atacctcgag ggcaaggtgc tcccgggtct ggctgttctg gatgaggaga
     2521 actgacaatg gcacgcaagc cgctgatggc cggcaactgg aagatgaatc tgaatcacgt
     2581 cgaggccacc gggctggtgc agaagctggc ctggacgttg acggacgcga agtacgaccc
     2641 ggagaggtcc gaggcagccg tgatcgtgcc gttcaccgac atccgcaccg tgcagaccct
     2701 ggtcgagggc gacaagctgc cgctgaagtt cggcgctcag gacgtgtcgg tgcacgcgtc
     2761 cggtgcctac accggtgaga tctcggcgga gatgctggcc aagctgaact gcgcctacgt
     2821 ggtggtcggc cactccgagc gtcgcgagta ccacggcgag accgacgagc tgatcggcga
     2881 gaaggcgaag gtcgtgttcg cggccggcat gacgccgatc gtgtgctgcg gcgaggtgct
     2941 cgacatccgc aaggaaggca cccatgtgcc gttcgtgctg ggccaggtcg aggcctgcct
     3001 ggacgggctc acggccgagc aggtggcggc catggtgatc gcctacgagc cggtctgggc
     3061 gatcggcacc ggcgaggtgg ccaccccgga ggacgcgcag gaggtctgcg gggccattcg
     3121 cggctacgtc gccgagactt tcggggtcga ggccggcgaa gccgtccgga tccagtacgg
     3181 cggctcggtg aaggcgaaca acatcgtcga gatcatggcg aagcccgacg tggacggcgc
     3241 cctggtcggc ggggcgagct tgaacgcgga cgagttcgcg aagatcgtga tgttctattc
     3301 cgtgatctga cccatccgcc gagggccagc gtaagcgtcg agaaccagcc tttagaactg
     3361 gttctcgacg tccaaggctg gccctcggcg cattctcggg ggcggacgga cggcatggcc
     3421 gaccagcggg gccggcgttt cgtcgctcgg gtgctcggtg agttaagatt ctgcgcgtga
     3481 ctcagctgcc cttgatgacc tggccgatcg tgacgctttc ggtcgtcctg atcctcacca
     3541 gcatcgtcct cgccctgttc atcctgctgc acaagagccg gggcggcggc atgtccgacc
     3601 tgttcggcgg cggcatgacc accggcatgg gcggcacctc cgtggccgag cggaatctcg
     3661 accggctgac cgtcatcgtc ggcctcgtct ggctggccag catcatcgga ttgctggtgc
     3721 tctaccgatt cttccccgag atcgggaccc tgggctgagc ccccaaaaca cctagcaagg
     3781 agaagtcagt ggctggtgga agtgcgattc ggggcagccg cgtcggcgcg ggtccgatgg
     3841 gtgaggcgga gcggggtgac gccgctccac ggctctacgt ctcgttcttc tgcgcgaacc
     3901 ggcatgagac gcgtccggcg ttcgccgcgg acgcggtggt tccggaaacc tgggattgcc
     3961 cccgctgcgg attgccggcc aacctcgatt ccgagaaccc gccgccaccg ccgaagatcc
     4021 agccctacaa gacccacctg gcctatgtga aggagcgtcg taccgacgcc gaagccgtgg
     4081 ccatcctcgc cgaagccctg gatgcgctgc gcgcgcgccg cgcggctggc gaggtcattt
     4141 actgatcgag ccgtcgccgg tccgagcgag gccgctcgaa cgcgttaggc ccagcagtcg
     4201 aagtacggga gcttgacggc tgcgtcctcg tcgatcagcc agagggtgca gtcggttccc
     4261 tccacatggg cggccggcag ttcgggatcg ccggcgagcg ctctggccag cgagtcaccc
     4321 ttctcctcac ccgacaccag gaaccaaacc tgccgcgagc ggctcagcgt cgcgagcgtc
     4381 aggctgatcc gctcgctggg cggctttggt gcgtcgctca ccccgatcac cggctgcgtg
     4441 gtcggctcgg acgacgggtg gttggggaag atcgaagcca catggccgtc cgggcccatg
     4501 ccgagcaggc agatgtcgaa gcgggtctcg ccgagttcct tggcataggt gttcgcggac
     4561 gcgcctgcgt ccaccacccc gtcggcgctc ggcatcggat gggtccgggc cgggtccacc
     4621 tggaagtagc gcgccagcag ggcgagcgtt ggcccggcat tgcgttcggg atcttcggtg
     4681 ggcaggaagc gttcgtcgcc ccaccacaac tccagccggg acgaatccac ctcgctgtcc
     4741 gccagcagtt cgcccaggcg ggcatacatc gcggtggcga tccggccgcc ggtgaggcag
     4801 aggtgggcca caccgccgtc ccgctgcaac tgggtgaggg tggccagcag tcgccgcgcc
     4861 gtggtgtctg ccagttcctc gtggctacgg tgccgcacca cctggggttg cctgccggtc
     4921 atttgcgcgc gccgccggtc tgcggagtcg cgcggtcgag tcgccgcacc agagctgcca
     4981 tggcctcagc gtagatggga tcgtcgtcca ttcgccgcag ctcctcggtg agcagggtgt
     5041 tcaggtcgcg ccgggggagt gccaccaggc gctccggcag ccccggtgcc gagatcacgg
     5101 ccaggttgcc gtcggttcgg gccacccgga tctcgtcggt ctcggcgcca agggtcacct
     5161 cggtgatgcc caccccggga tgtcgggtga ccgcgacgcc gacgttcagg cgatcgccca
     5221 gccaggccgc gagcaggctg gcggcagcat tgtcgggcgc cgcccccacg gtcgcgcggg
     5281 tcacgttcat cgggtaggcg tccagtgcgg cagcgagcag gccgcgccag cgggtaagcc
     5341 gcgtccaggt gaggtcggtg tcgcccgggc agtagttgcg agcccgcacc tccagcgcgg
     5401 cgagcgggtc gggatcaccc atcgcgtcgg tgatccgacg cgtgcccagc cgtccgagct
     5461 ggtcggacgc tggcgccacc ggcgagcagc ccgaccacca gacgatcacc ggcaggtcgg
     5521 gcaggagcag cggcagcacc accgaatccc ggtgtttgcg cagctcgccg tggaacttga
     5581 gggtcacgat ctcgccgggc gcatcctcac cggcgcggat ggacgcgtcc agtcggtccg
     5641 cgcgggctcg tccgttcgtc accagcagga tccgggatgg atgctcgagc gcggtgtcaa
     5701 ccaccgcagc catggcccgg tcgtagtcac cctggtcgac gaccacaacc agggtgaaga
     5761 ccacgccgga gatccccccg agccgatggc gcagttgccc gagctgctcc tgcagcgccg
     5821 cggcgttggt gtcagtgagt tgcacgatca tcttcggtct cctcaagccc ttacggccgg
     5881 cgccagctga agccgtcgcg ggcgagcatc tccacagccg acgtcggccc ccaggtgccg
     5941 gatgggtagg cctggggcgg ttcctcctgg ctggcccagt ggtccaggac cgggtcaagc
     6001 agccgccagg acagctccac ctcagccggc tgggggaaca gcggctccgc gccacgcagc
     6061 acgtccagca gcagccgttc gtaggcctcc gggctggact ccacgaacga ggtgtcgtag
     6121 ccgaactcca tgttcacctc gcgtagttcg gaggtggccg gcaccttggc gccgaaccgc
     6181 agcgtgatgc cctcctccgg ctggatccgc agcaccaggg cgttgttgcc gagcgagcgg
     6241 acgtccgcgt ggtcgaaggg caggtgcggt gcggtgcgca ggttcagggc aacctcggtg
     6301 acccggcggg ggagccgctt gccggcccgc aggtagaacg ggacgccggc ccagcgacga
     6361 ttgtcgatgt ccacccggat cgccgcgaag gtgtccgtgt gtgagtcggc cgggatgccc
     6421 ttctcctcca gatagccggc caccagccgg ccaccggccc aacctgcggc gtactggccg
     6481 cgcgcggtgt gggcgcccag atcggccggt gtgctcgccg ccgccagcac cttgcgcttc
     6541 tccgcgctca actcatcggc gccgaaagac gtgggttcct ccatcgcggt cagggccagc
     6601 agctgcagca gatggttctg gatcacgtca cgggccgcgc cgatgccgtc gtagtagccg
     6661 gcccggcctt cgatgccgat gtcctcggcc atggtgatct gcacgtggtc gatgtagttg
     6721 cggttccaga tcggctcgaa catggtgttg gcgaagcgga aggccagtag gttctggacg
     6781 gtctccttgc ccagatagtg gtcgatccgg aagacgctgg tgggctcgaa cgccttggcg
     6841 agcttccgct ccagctcctg cgcgctggcc aggtcatggc cgaacggctt ctcgatcacc
     6901 acccgccgcc acgagcctct cggccggtcg gcgagaccgt gccgacgcag ttgcgcaacg
     6961 gtgatgtcga aggcagttgg tggcaccgac aggtagaagg catggttgcc gccggtgccc
     7021 tgggtttcgt ccagctcggc gagggtgcgc ttgagttcct cgaaggccgc gtcgtcgtcc
     7081 agttcgccgg agacgaaccg gacgccggcc acgagctgcc gccagacgtc ctcacggaag
     7141 ggcgtccggg catggctgcg gacggcgtcc gccacgacat cggcgaagtg ttctgccgcc
     7201 cagtcgcgcc gggcgaaccc gaccagcccg aaaccagggg gcagcatgcc gcggttggcg
     7261 aggtcgtaga tggccgggag gagcttcttg cgggcaaggt cgccggtcac gccgaacagc
     7321 accagggccg aaggtccggc catctcggga agccggcggt cgcgggggtc gcgaagagcg
     7381 ttcaccgtca cgactgtaca agtgcaagct ccgcgagcgg ttcacactga tcgcagtcca
     7441 gaagagcttg ggtctagact gctcagacct tctcagagag gacggcatga gaccagccga
     7501 ggaaggcgcg cccgtctcgg tgctggcgcc tgttgactgg cgagcgaccg cgaaggccta
     7561 tctggccctc gcgaagccgc ggatcattga actcctgctg gtgaccacgg tgcccgcgat
     7621 gttcctggcc gccggcggct tcccgccctg gcagctggtg ctctggacgc tgctgggcgg
     7681 taccgctgcc gccgccagcg ccaatgtctt caactcggtg ctggacgcgg acatcgacgc
     7741 caggatgcgg cggacgcggc ggcgtccgat ggcgctcgac ctggtcacgc cggcgcgcgc
     7801 gatcgccttc ggcgcggtgc tcggcgtcgc gtccgtcctc atcctggggc tgggcgccaa
     7861 ttggctttcg gcggggctcg ccctcgcggc gaacctctac tacgtgttcg tctacacgat
     7921 ggcgctgaag cgccgcacct cgcagaacat cgtctggggc ggcgtcgccg gatgctttcc
     7981 gccgctgatc ggctggacgg cggttaccgg ctcggtggcg tgggccccgc tggtgctgtt
     8041 cgcgatcgtc ttcgcgtgga cgccgccgca cacctgggcg cttgcgctgc gttaccgcga
     8101 ggactacctc aatgcgcacg tgccgatgct gccggtggtg gcgccgccgg tgacggtggc
     8161 ttggcgcatt cttgcttata gcgcgctcac catggcggtg agcctgctgc tctggccagt
     8221 ggccggaacc ggctgggggt acccgctggt cgcgctggtg gctggactgg cgatgctggc
     8281 cgaagcggtc gcgttgctgg ttcgagccaa gcgcggcctt accgacgcag cactgaagcc
     8341 gatgcggctc ttccacctct ccaacagcta cctggcgatc gtgttcatcg ccgctgcggt
     8401 cgatccgttg ctgttctgag cgacgcgagc accgctcgcg tcagccggct cgatcagtca
     8461 aacccgattt ggtaggcata ccaggccgcc ggcggacctc cctagggtct cgacccgacc
     8521 tggatggctt atccgggagc tgcggtgccc cgtaagccat ccaagtcggg tggcgccatc
     8581 tgtgcccggc tagtcagcct gaataagcca tcaaactcgg gtttcgtgcc ggtacgcggg
     8641 ccagccacca gacgttggcg atcgctgcgc agaacagcgc cgtcccaagc atgtgcaggg
     8701 ccaccagcat cggcggcacg ccgaggaagt actgggcgta accgatcgct ccctgcagca
     8761 cgaccacacc gagcaacagc agcgtcgcac ggaacagcgc cggcgtacga cgggccagcc
     8821 aaagcagcgc cacgatggcg atggtcgccg cccacaccga caatgagtgc gtccgggcta
     8881 ccgcagtgag gtcgaagccg ctgcggtgca cgtccgcgtc gccggcgttg ggtccggcgc
     8941 cggtcacgat gatgcccagc agtagcacca gccagcccaa cgccaggacc acccacgcca
     9001 gattgcgggc agccgcggtg gcgccagcag gtgcggcgcc gaagacctcg tgcaccagca
     9061 ccgtgcagac catgatcaag gcgagtgagg gcaccaggtg cagacctacg acccaccatt
     9121 cgagccgcgc cagcaccgag atgccgccga tgacggcctg cgctgggatg cccgctgccg
     9181 ccagcagagc cagtacccgc acccgtcgga cggggttgcc ggcggcgtcc ctggcccgcc
     9241 aggccgcgat cagcgtcccg atcgcaaccg cgaccagcac gaaggtcagc agccgattgc
     9301 cgaactcgat gtaggtgtgc agcggagcat ccccgtgcgg cacgtacgag cccggctcgc
     9361 actgcggcca ggtggagcag cccaggccag agccggtgac ccggaccagc ccaccggtca
     9421 ccaccaggcc catgttcgcc accagtgagg cgatcgacca gccgcgtagg gcagcggggg
     9481 agccaactag atccatcgga acaccttcct ggcgagcgcg agtcccgcga ccgcccagat
     9541 cagcacaacc agcaacggcc acagcgctac accgccggca gcccagaccc gaagtgactc
     9601 ggcaaccgcg gtcgtcggca gcgacgcaac caggggctgc gcccaggcag ggaagctcgc
     9661 caccggtacc aggatgccca gcgcggcccc gccgaggtac accaggttcg ccaggccgag
     9721 ggtcagttcg gcgctgaccg tccccgccag gcacagggcg aggctcgcaa aagcgaccag
     9781 cccgagcagc accaccagca gggtcacccc gctctgcaac agggtcggcg ccggtcgcca
     9841 accgacgatc aatccggcca gcgcaagcac cgctgcctgg ccgacagcca ggatcgcgat
     9901 catggtcgcc ttaccggtca gcagatcggc gcggcgcagg ggagtggcca ccagccgctc
     9961 cagcacgccg taacgccgct cgaaggcggt ggtgatcgcc ggggaggtga acccgctcga
    10021 ccacagtccg agtgcgatta ccgaagccac gaacggctcg cgagcaatgc cgaaccgcgg
    10081 gccgagcacc gcctgcgcga cgatcaggcc cagaggtatc accaatgcca ggagcaactg
    10141 ttccccgttg cggacgatca gccgggtctc catgagcgaa tgcgctatga cccgccgcgg
    10201 acgggtggcc ggggcagccc cgggaccgag gtcgaggttc atgccgcacc gcccggggtg
    10261 gtgaggctca ggaagagctg ttcgaggttg ccgtcggcgg tcagctcccc gacggtgccg
    10321 gacgcgaccg cgcgtccggc gttgagcagg atcacccgat cggccagctt ctcggcctcg
    10381 tccatggcat gggtggtgag caccacggcc acgccggcgg cgcggaggtc gcggatcagc
    10441 tgccagaccg tgcggcgcac gtgtgggtcg agcccggcgg ttggttcgtc aaggaagacc
    10501 agctccgggc ggccgatgat cgccccggcc aggttcacca gctgctgctg tccgccggaa
    10561 agccggcggt agggagtctg cgcgaaggtg ccgatgccga gcatctccac cagttcggcc
    10621 accggcagcg gggacgcgta gagcgcggcc agataccgca ggagttccag cggcctcacc
    10681 ccggaccagg cgccggtcgc ctgcggcatc aacccaaccc ggtcattcgc cccgggtgcg
    10741 cccgcggccc ggccgagtac atcgatcgtg ccggcgtccg gactcagcag cccggtgcag
    10801 cagcggatca tcgtggtctt gccggccccg ttagggccga gcacagcggt cacctcgccc
    10861 accgcagcgc tgaggctgag cccggtcagc accggacgcc gcccaagctg gatccgcaca
    10921 tcggtcaggg tgagggcggc ggacacgcgc tcagtctagg cccgggccgc ccgggcccgg
    10981 ccccgcacgc aaccccgggc cggaaaacgc tgctgcccgg caccctcgcg ggtaccgggc
    11041 agcagctggt taacagcagg gtcagacctt cgctgcgtcc gccacgtgct cggcggccgc
    11101 aggagcgggt gcaccggtgg acagctccgg cgagggagcg tccttcaggt aggcgaagtc
    11161 cgcagcggtg agcatcggct gatcgatgcg catgccgtag aacgaacgcc acaccatgta
    11221 ggcgctggcc aggaagaaga ccacggctag gacggcgttg atcgcgctgt tgttgatgat
    11281 cagcgacagc tcgatcgcca gcatgccgaa cagggtggtg aacttgatca ctgggttcag
    11341 ggccaccgag gaggtgtcct tgaacgggtc gcccacggtg tcgccaacga tggacgcatc
    11401 atgcagttcg gtgcccttgg cctgcagatc gacctcgacg atcttcttgg cgttgtccca
    11461 ggcgccaccg gcgttggcca tgaagatggc ctggtagagc ccgaagacag cgatcgagat
    11521 caggtagccg atgaagaagt acggctccac gaacgcgaac gccagcgtgg cgaagaagat
    11581 gccgaggaag atgttgaaca tccccttctg ggcgtactgg gtgcagattt ccaccacgcg
    11641 gcgggagtcc ttgaccgaag ccttctccga ggtctcaagg ttgatgttgc ccttgatgaa
    11701 ctccaccgcc cggaacgcgc cggtggtgac ggcctgcatg gaggcgccgg tgaaccagta
    11761 gatgatggcg ccaccggtga tcagaccgag caggaacggc acgtgcaaca gcgacaggtt
    11821 cgccagcagg tcggtctgca ggccgttggt gaggcccatg atgatcgaga acaccatggt
    11881 ggtcgcaccc actgccgcgg taccgatcag cactggcttg gcagtcgctt tgaacgtgtt
    11941 gccggcaccg tcgtttgctt ccagcaggta cttggcccgc tcgaagttcg gggtgatgcc
    12001 gtagtcgcgc tgcagttcgt cctcgatgcc ggggatctgc tcgatctgcg acagctcgta
    12061 gacgctctgg gcgttgtcgg tgaccggtcc gtaggagtcg accgcgatgg tcactgggcc
    12121 catgcccagg aagccgaagg cgaccaggcc gagggcgaac acgcccggag ccagcatgat
    12181 gccgccggct gcggcgttgg ccgggtcgat ggcgggaatc atggtggaca cgccgtaggc
    12241 gccgcccatc agaccgacga tggcgatgcc cagccagtag gcggagaagt tgccggcgac
    12301 caggccggac aggatgtcca gggacggacc gccctccttg gccgacttaa cgacctcctc
    12361 gacgtgcttg gccttgaccc cggtgaacac cttgacgagc tcggggatga tcgcgccggc
    12421 cagtgtgccg aagctgatga tcagcgagag ctgccaccac aggttggtgt tgccacccac
    12481 gttcgggatc agcatggccg agatgatgaa ggtgagcacg atcgagacga tcgaggtgat
    12541 ccagaccagc gaggtcagcg gggtctcgaa ctccatctct tcagcattgc cgtacttcga
    12601 cttcgcccag agcgcgttgc cgaagtagga aagggccgat gcgaccagca tgatcgcgcg
    12661 gatgaagaag atccagacca ggaagtgcgc ctgcaggtcg gcctgcaccc ccagcaggat
    12721 gaaggtgatc agcgcgacac cggtgacgcc gtaggtctcg aagccgtcag cggacgggcc
    12781 gaccgagtcg ccggcgttgt caccggtgca gtcggcgatg gtgccggggt tacgggggtc
    12841 gtcctcgccg atcttgaaga cgatcttcat caggtcggag ccgatatcgg cgatcttggt
    12901 gaagatgccg ccggcgatcc gcagtgcgga cgcgcccagc gactcgccga tcgcgaagcc
    12961 caggaagcag gcggtcgcga tctcggcggg caggaacatc atgatcgcga gcatcatgat
    13021 cagctcggtc gagatcagca ccatgccgac gctcatgccc gaacgcatcg ggatgtcgta
    13081 gacgttgaac ggcttgccct tcagcgccgc aaaggaggtg cgcgagttgg cgaaggtgtt
    13141 caggcggatg ccgtaccagg cgatgccgta cgagccggcc atgccgatga tcgcgaacag
    13201 cagcacgatg atcacccggc cccagccccc ggtggtgttc aggaagccga agtagacgaa
    13261 gatgaccgcg ccgatgaagg cgaacagggt gagcaagaac ttgccctgct gcagcaggta
    13321 ggccttgcag gtttcgtaga tcagctcgga cacctcccgc atggactggt gcaccggcag
    13381 gttcttcagc ttcatgtagc tgtagatgcc gaaaccgaac ccggccaggc agatcagcat
    13441 gccgatcagc agcaggtacc agcccgaaac gccgccgagg aaggtgaccg cgtgcaggtc
    13501 ggggagcggt aggtcgaact cgctcttgtg ggtggtgtgg gtgggctcag cgccggtgcc
    13561 ggaacaggca gccagcgaca gtcccaagag cccgaaaacc acgaacaggc ccgaccgcac
    13621 caaactgtgg ggacgacctg cggatggttt ggtatcgagc gatgaagtcg tcacgatgca
    13681 tccttcaatc aggtggttgt tttgctgatg tccgtctcgc cgttgttaga acaaacatcg
    13741 cccagcactc tacgaccatg tgtcgtagtc accgaatcaa ggccaccttt tggatgggcg
    13801 tctcatcccg ttggcggatc atgcctggac gcccgggttg agctaagcct cgattaaggc
    13861 aagaatgacg ttgtgaaaag cgagaccgag ccgaacgggg acgcggcggt agccgaggcc
    13921 gagcagcagg ccgcggacgc ttccacccgc agccgagtgg cccgctcgat cctgcagcac
    13981 ggaccgtcca ccgccaacga cctcgcgagt cggctgggcc tgaccccggc cgccgtccgc
    14041 aggcacctca ccgtcctggc cagcaccggc caggtgacca gccgcgagca gcgcgtctat
    14101 gggcaccgcg gacgcggccg tccggcgaag gtcttcgcgc tcaccgactc cgggcgcggc
    14161 gagttctacc aggcgtacga cgccctggcg atcaatgcgc tcgaccatct ggccgaagtg
    14221 gccggcccgg aggcggtgga agccttctcg gccaaggtcg ccgaggccgt ggccgcccgc
    14281 ttcgcgatca tcgcaccgga atacgagagt gctgccgatg cgttggttga ggcattgact
    14341 gccgagggtt tcgtcgcctc actgcagccg gtcgcctccg gcgtccagct gtgccagtat
    14401 cactgcccgg tcgcccacgt ggcggctgag tacccgcagc tctgcgaggc cgagaccagg
    14461 gtctttgcca ggctgctgga ttcgcacgtg cagcggctgg cgacgatcgc ccatggcgac
    14521 ggggtgtgca ccacgcacgt cccacgtccg gtggtcgccg cgatgtaccc atcaccaaac
    14581 cgaagggcca actgatatga cctcaaccac cacggctccg gggcttggct ccacccagga
    14641 agaacacctg gaagcgctgg gcacctatca gtacggctgg cacgactccg acctcgccgg
    14701 gacttccgcg aagcgggggc tgaacgagga cgtagtccgc aacatctcgg cgctgaagtc
    14761 cgagcccgag tggatgctga agctgcggct gaaggccctc aagctgttcg accgcaagcc
    14821 gatgccggcc tggggcgccg atctcaacga gatcgacttc gacaacatca agtacttcgt
    14881 ccgctccacc gagaagcagg cccagacctg ggaggatctg cccgacgaca tcaagcgcac
    14941 ctacgaccgg ctgggcatcc ccgaggccga gaagatgcgc ctggtggccg gcgtggcggc
    15001 gcagtacgag tccgaggtgg tctaccacaa gatcaacgag gaactggagc gtcagggcgt
    15061 catcttcctc gacaccgaca ccggcctgaa gcagcatccc gagatcttcg aggagtactt
    15121 cggctcggtg atcccggtcg gcgacaacaa gttcgcctcg ctgaacacgg cggtctggtc
    15181 gggcggttcg ttcgtctatg tcccgaaggg cgtccacgtc accatcccgc tgcaggccta
    15241 cttccggatc aacaccgaga acatgggcca gttcgag
//
LOCUS       DAOPRQ010000061        13899 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_3717_length_13899_cov_12.200448, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000061 DAOPRQ010000000
VERSION     DAOPRQ010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 13899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13899
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3717_length_13899_cov_12.200448"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..117)
                     /locus_tag="PKJ61_08760"
                     /pseudo
     CDS             complement(1..117)
                     /locus_tag="PKJ61_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010078638.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            227..457
                     /locus_tag="PKJ61_08765"
     CDS             227..457
                     /locus_tag="PKJ61_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13953.1"
                     /translation="MTAAQGVEKHSVSMPAATSESVRSRVGARGFSGYVAAAVARQLE
                     RDALDDALARMEAEHGRVDEDEVAAIFARLAG"
     gene            454..867
                     /locus_tag="PKJ61_08770"
     CDS             454..867
                     /locus_tag="PKJ61_08770"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HOC13954.1"
                     /translation="MIVRPHSILLDAEALSALARDDRRLQAWATFAKRSDSVIYVSCL
                     TLAEASDGTAREVAVRRAVKAVRLVDVGPDLGFRAGQLRATASASRRKRRDLTVDAVV
                     AATALALPTPVVVLTSDPEDLGLLLAGHDVRIEAV"
     gene            complement(1325..2191)
                     /locus_tag="PKJ61_08775"
     CDS             complement(1325..2191)
                     /locus_tag="PKJ61_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012924242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOC13955.1"
                     /translation="MPETTTHVIEMPGAVVVYDVHEPDQPSDHRPLFIFGSPMGAAGF
                     TELVRLFGDRTVITYDPRVGERSRLEKGAALSYEVHGDDVHRVVAASGLGPVDVFASS
                     GGAVAALPWLLAHPQEVGTVVVHEPPLTALLEDGEVLERVMADIAATYQAQGQGPAMA
                     KFIQLVMYDGPLPADFLDRPAPDPALFGFSAEDDGSRDDALLGRNLTMAAYHADGHAL
                     RASGVRILPAVGAESGAAMPRRAGEALAGLLGVEAVEFPGDHGGFATNEWSPQNDPAV
                     FAAKLREVLDGD"
     gene            2459..3850
                     /locus_tag="PKJ61_08780"
     CDS             2459..3850
                     /locus_tag="PKJ61_08780"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOC13956.1"
                     /translation="MNAELELLGGGAEAAELLLSRYDVADGRWDVPKEGDFWDAVGSA
                     HDLGREGAGKRVAVIDGGFDLSIPALSGQALRFAQGEGEAATHGTVVALLVREVAPAA
                     SLDLYPVSVAGKLNLDRVIAAIADCVQRGVDVINLSLGAALAEETVSTMPTGTDASPA
                     DTSRTPNPAYASDALEPDDWRTIFDVPESPLWRAAFDAANAGVTVVAATGNYAGTVYI
                     PAATPGVIAAGFLTVERLVSNRLEEAAAKPPAFIQSRYADLQLLQPADVLGSSFASPL
                     IAAFATLMADRKELPAYVRCARRAANASGALRLLQEGESANSDFLMLTDSLFKGALAS
                     APHTHYDEDVYSITPCPECAVFANPAYNDFGLFTMSTGNVDIAVRLFASLRQIAPTNP
                     YAAANLGVAFAMLAQAAKDAGDLERTRVLLEAAVGHVGDAVGLRPGYAPYAARLAEFE
                     AARVDPTKWTLAR"
     gene            3883..4359
                     /locus_tag="PKJ61_08785"
     CDS             3883..4359
                     /locus_tag="PKJ61_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13957.1"
                     /translation="MAVDRISIKIEDNDDLTAAYAAASATGVPVQAVTPPPSGDLEAQ
                     IEPVTAVLIGAGVVAAVKLILDWWERRKGGLVIDLRKDATDMFYRDKDLTYGFVATIP
                     EKGGKVTVDVKDLPDAATTWINLVITSAFKTVQDAAKAAKAAVGDSKVSVEPAAAG"
     gene            4435..4695
                     /locus_tag="PKJ61_08790"
     CDS             4435..4695
                     /locus_tag="PKJ61_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13958.1"
                     /translation="MPKRRWSDLSAGTRAAIVILGAADLALRAWSIADLSRRPQAQVN
                     GPKLLWMTTLSVVSSMGLLPAVYLSWGRRAGAAGSRRKRRTP"
     gene            complement(4705..5451)
                     /locus_tag="PKJ61_08795"
     CDS             complement(4705..5451)
                     /locus_tag="PKJ61_08795"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HOC13959.1"
                     /translation="MTGHQDDAAPATEQSLNAEPRGFETVMAYLTQAIIDRKLPAGSK
                     LPTERQLATDLDVSRSAVREAIKVLQAQGIAATAAGRAHGTRITTTPSEAFGRMLSLQ
                     LALGSTDVDELTDTRVVLERAAISEAARRATPDSLEEARTLAWRMTLATGSAEFNDLD
                     TAFHVALAQVGQNRLISDFTVAIRQAVAPMIRDAQTTVADWEVLRRRLATEHLEILET
                     VAEGRDEAAADLVESHIRRAHADLHLQLPS"
     gene            5622..7322
                     /locus_tag="PKJ61_08800"
     CDS             5622..7322
                     /locus_tag="PKJ61_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015583423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HOC13960.1"
                     /translation="MVPLEAFLPDTNPTGMGWLSALLAALPIVAMLVTLGALRWKAHL
                     AGAFSWVVAFVVAIAAFRMPALMAVSTSVEGFLYGLFPIVWILLTAIWMYDITVVSGR
                     FDDLKRTFFLLSDDPRVLALLIAFCFGGLLESLAGFGAPVAITAAMLVAIGFSPLRSA
                     MTALLANTVPVAFGAVGLPVIMAAKVGEFDNVLPISTISGRITAILCLVVPFLLLAVI
                     DGKQGIKEVWPFGLVVATSFGVTKWIVSSTPLYNLTEAVAAVVSVAVAIAFLRFWKPT
                     GGAAAIDRVARPIDPRIESHLPAPAPTDPDAPALTRNRILMALVPYALVIVVFSIAAI
                     PQVKAALAGTDLKLHWPLLDEMLNTAGAPAAHQTYTFAWASTPGILLALVAILTGLIY
                     RMGWKRIFKVLVDNMVKLRFTALTIGSVVALAYVMGDSGQTMALGLFIASSGALYPFF
                     APVLGWIGTYVTGSDTSANILFSGLQSGVGHQIGGDGAHLDVLQMRTLLVGGGGIGGV
                     VGKMISPQSLSVASTAIGLPGSESAILRRMIPWSLVLLVLLCLLSGLMSTPALSWLIP
                     "
     gene            7355..8146
                     /locus_tag="PKJ61_08805"
     CDS             7355..8146
                     /locus_tag="PKJ61_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002517115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOC13961.1"
                     /translation="MSVSTGTAAKVGKPGLGKRVALFATCINDAMMPAAPEATVRLLE
                     RLGCTVEFPRQQTCCGQILTNTGYFDEAVGTVKAYVGAFSDYDYIVGPSGSCVASVRH
                     QHPMLARHSGDSGLEKAATAAASRSYELTEFLVDVLGVVDVGAYFPHRVTYHPSCHAT
                     RMAKIGSRPYQLLEAVRGLELVPLPHAEECCGFGGTFSLKNPDISIAMASDKARHVRS
                     TEAEYLVAADNACLLNIGGVLHRQFSGIKTIHLAEILAQTEQDAA"
     gene            8143..9636
                     /locus_tag="PKJ61_08810"
     CDS             8143..9636
                     /locus_tag="PKJ61_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016669553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HOC13962.1"
                     /translation="MSLDAQRRTPAPPPGGWDGLPVFPKAVKVELQLTEQRKNLRHAT
                     GTIRAKRALRASEVPNWEELRDAAAAIKDRVGRHLDSYLEQAERAMLDAGITVHWARD
                     AAEANQIVVDIAKAKGVDEVVKIKSMVTQEIDLNEHLERSGIAAWETDLAELIVQLGH
                     DLPSHILVPAIHRNRSEVREIFTEEMGRYGLAAPSGISDDPAELAAAARAHLREKFLR
                     AKMAVSGGNFIVAETGTLVIVESEGNGRMCLTLPETLVSVVGIEKILPTLADLEVFLK
                     LLPRSSTGERMNPYTSFWTGVTTGDGPQDLHVILLDNGRSRVLADPVGRAALRCIRCS
                     ACLNICPVYERVGGHAYGSPYPGPIGAILNPQLHGVSDPKDQALPFASTLCGACNEVC
                     PVRIPFTDILVQLRHKVVEYKTGKHPTVEQGIMKTVSWVMSDGKHLEKAELATRAAGR
                     VLGDRVLGPMPVPLAGRWLRARDVDAPPVETFRQWWKHHRAADGKEL"
     gene            9636..10289
                     /locus_tag="PKJ61_08815"
     CDS             9636..10289
                     /locus_tag="PKJ61_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013161708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HOC13963.1"
                     /translation="MNAREEILGRVRAATQDVTVADPALDVPIAWTYGQPLATADVLA
                     DFVEKVEDYRATVVRVPAVEVPRAVAEALVTLGATSAVLPSGVPATWAQAVAEAGVQL
                     FADEPQLDYATLNTVSAVVTAAAVGMADSGTIALDHGDDQGRRVLSLLPDIHVCVVRA
                     DQVVSDVPEGVARLSPGLRAGRPVTWLSGGSATSDIELSRVEGVHGPRTLWVVLAEG"
     gene            10405..11805
                     /locus_tag="PKJ61_08820"
     CDS             10405..11805
                     /locus_tag="PKJ61_08820"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HOC13964.1"
                     /translation="MGSSTALRRLLAVVVAVFLTLAGLLAPVTPAQAATKPKVTGISI
                     TSGNLQGGETLTIRGTGFKKVKRVLFGTVRATNVKVKSSKRITVTVPPGQAGVVDVRV
                     VTKKGTSKRTTRAKYRYLAAEPAPVPSAAPTVSGIAPALGSVSGGTVVKLTGSRFAGA
                     TQVLFGSRPGTGLVVASDSAITVTTPSGVQGAVVVKVVGPGGTSAGTSFVYQLVDTMS
                     AGQRLSAGQSLSSSNGGYRLTMQADCNLVVYTSAGAAKWGSSTNRNDAGCHADVLADG
                     NLVIYTSGGQAVWASNTGGFNGAALRMQDDGNLVIYHGSAAVWSSKGGSGKVSSFKAW
                     ALNSANWNSTTRIYDAAGNIVGGGKPGIDADGAFGAQCADLGIAWSGQAGHRVGFDGW
                     DSSTAGKAGWHFVSGNLSQVQPGDVVTRVGGKQHVVVVTGSPSGGSVEVIQQNPGSPA
                     VASYSTSTSGVIWRLN"
     gene            11884..12594
                     /locus_tag="PKJ61_08825"
     CDS             11884..12594
                     /locus_tag="PKJ61_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020575371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HOC13965.1"
                     /translation="MTPEPIELFIRAVDLTGVFANAILGGLIARAKRFDPVGFLTLAI
                     TSGLGGGMIRDVLLQVGPPLALTDSAYLLVAIAGTAVAFFVPVSKRLWDLSFPYLDAL
                     ALGCWSAAGASKALAAGLGWLPAVMMGTITAVGGGVLRDVVLRKTPSVFGGNTLYATS
                     AILASIVLVVLQPLTVAPTATLTAIGTGMLVTLLARRLEWRLPSSYSWSPKRAVAMLP
                     RPRWRPKRKRTRPRADEA"
     gene            12646..12798
                     /locus_tag="PKJ61_08830"
     CDS             12646..12798
                     /locus_tag="PKJ61_08830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13966.1"
                     /translation="MHSPSSWRARLAKRIDPATVRNRVENVNDGILAIAGFSEGLAGG
                     GEIQNL"
     gene            12943..13341
                     /locus_tag="PKJ61_08835"
     CDS             12943..13341
                     /locus_tag="PKJ61_08835"
                     /inference="COORDINATES: protein motif:HMM:NF014090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - cellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HOC13967.1"
                     /translation="MAEIATYYEGRGVTPAIAQRVAEELNAADSLGAQPQIDGFGKPT
                     AVSGAISVALGAGVAFLIGATVPILISVITPVTWRDEYTILAVAVALGFTSIILARLG
                     HTRIWQPLARSVFIGLAALGSSYFVGSMLL"
     gene            complement(13367..13795)
                     /gene="trxA"
                     /locus_tag="PKJ61_08840"
     CDS             complement(13367..13795)
                     /gene="trxA"
                     /locus_tag="PKJ61_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013161660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOC13968.1"
                     /translation="MSSRVAVCPACGTKNRVPDAAKGRPQCASCHQALPWLVDASDAG
                     FASAIDTDQLVLVDLWAPWCGPCRMVAPILERLAASYAGRVKVVKVNVDDNRRTATRF
                     DASSIPTLVMLRNGKNVARFVGAQPEQVLRKRIDALLASG"
BASE COUNT         2186 a         4752 c         4613 g         2348 t
ORIGIN      
        1 gtcgaggccg aggtagcggc gggcttcggc ccattcgtcg tgttgttcgg ccaggccggc
       61 gcccacgagc cggatgatgc tggtccggtc agggaagatg ccgacgacgt cggtgcgggt
      121 gccgtgaacg tccgcatctg ccgcactcgg cgcgggcctg aaagacagtc gtggagcctt
      181 gccggagcaa tgacacccag attcctactt ctagtaggat ggagtcatga ccgcagctca
      241 gggagtagag aagcactcgg tgtcgatgcc ggccgcgacg tcggagtcgg tgcgttcgcg
      301 ggttggtgcg cgtgggttct ccgggtatgt ggcggcggcg gtggcccgcc agctcgagcg
      361 tgatgcgctg gacgatgccc tggcccggat ggaggccgag catggccggg tcgatgagga
      421 tgaggttgcc gcgatcttcg cccggcttgc gggatgatcg ttcggccgca ttcgatcctg
      481 ctcgatgccg aggcgttgtc ggccctggct cgggacgatc gtcgtttgca ggcgtgggcc
      541 acgttcgcca agcgcagcga ctcggtcatc tacgtctcct gcctgacgtt ggccgaggcc
      601 agtgacggaa ctgctcgcga ggttgctgtt cggcgggccg tgaaggcggt ccggctcgtc
      661 gacgtcggtc ctgacctcgg cttccgtgcg ggccagctgc gggcaaccgc gtcggcgtct
      721 cggcgcaagc gccgcgactt gacggtcgac gcggtagtgg ccgccactgc actcgcgctg
      781 ccgacgccgg tggttgtgct cacctcggac cccgaggatc tgggcctcct gctcgccggc
      841 cacgacgtcc ggatcgaagc tgtctagagt atgcctgtga tcgtccgtag gcagctgcca
      901 agatcagtcg cggatgaacc cagttcgcgg gtcgctcgag gacctcgaca agcgggctat
      961 cggggtgaaa atccgcgtca ctcctgacgc acccgggtga catggaactg tataacactt
     1021 agtcacgtgt ctcttagcga cagacttttg atccgcgggt tttgggttcg attcccagtg
     1081 agcccaccag gtcttcgatt cactcggatg gaccgaggaa ttgaggtcaa cggcgaagcg
     1141 aaggatcgtc gatgacctgc ttgattcagc acccgctgtg agaacgagta ctcggtctga
     1201 gtcgacggca gagtatctgg acggttgcga ccaggttcca ccagcgcggg tacctacctg
     1261 cgccaggaga cccgagccgg atcacgcgtt cggcgacgga ccggtgctcg tggcaacaac
     1321 ctgactagtc cccatcgagt acctcccgca gcttggccgc gaagaccgcc ggatcgttct
     1381 gcggcgacca ctcgttggtg gcgaaaccgc cgtgatcgcc ggggaactca accgcctcca
     1441 cgccaagcaa ccccgcgagc gcctcgccgg cccggcgggg catcgccgct ccgctctccg
     1501 cgcccacggc gggtaggatc cgcacgcctg acgcgcgcag cgcgtggccg tccgcgtggt
     1561 aggcggccat cgtcaggttg cgtcccagga gcgcgtcgtc gcgactgccg tcatcctcag
     1621 ccgaaaaccc gaacagggcc gggtccggcg ccggccgatc cagaaagtca gccgggagcg
     1681 gtccgtcgta catgacgagc tggatgaact tcgccatcgc cggtccttgc ccctgcgcct
     1741 ggtaggtcgc cgcgatgtcg gccatcacgc gctcgagcac ctcgccgtcc tccagaaggg
     1801 cagtgagcgg cggctcgtga accaccaccg tgccgacctc ctgcgggtgc gcgagcagcc
     1861 acggcaacgc ggcgaccgcg ccaccactgg acgcgaacac gtcgaccggg cccaacccgg
     1921 acgccgcaac gacccggtgg acatcatcgc cgtgcacctc gtaggacaac gcagcgccct
     1981 tctcgagcct gctccgctca ccgacccgcg ggtcgtaggt gatcaccgtc cggtcaccga
     2041 aaagtcgcac caactcggtg aaacccgcgg cccccatggg cgacccgaag atgaacagcg
     2101 gtcggtgatc actcggctgg tccggctcgt gcacgtcgta aacaacaacg gcaccgggca
     2161 tctcgataac gtgggtggtc gtctcaggca tgactcatcc tcagccgaag cgaccagcca
     2221 ggcaagaccc acaccgcaag cttgagctga gacctcgaga ttcgccgtgg aacgctacgt
     2281 cagagtgggt cgaaccttgc tttccgcggt acgcggctag cctgaccgac ctcaggttcg
     2341 gcgccgaact ggaagggccg gaaagggctg cacggccatt cccctcgaaa cagttagcac
     2401 ctagtgtttg agtcactcag ggtccgcacc ctgaacagcg gcagtccggg ggcgccagat
     2461 gaatgccgaa ttggagttgc tgggaggcgg cgcggaggcc gccgaacttc ttctgtcccg
     2521 atacgacgtt gcggacggcc gttgggacgt ccccaaagag ggtgacttct gggacgccgt
     2581 gggctccgcg catgaccttg gacgcgaagg cgcaggcaag cgagttgccg tgatcgacgg
     2641 cggcttcgat ctgtcgattc cagcactctc cggccaggcg ttgcggtttg cccaggggga
     2701 aggcgaagcc gcgactcacg gcacagtggt ggcgctcctg gttcgtgagg tggctccggc
     2761 cgcctcgctc gatctctatc ccgtgtccgt ggccggaaag ctgaacctgg atcgtgtgat
     2821 cgcggccatc gctgactgtg tgcagcgtgg tgtggacgtg atcaacctca gcctcggcgc
     2881 ggcgctggcg gaggagacgg tgtccaccat gccgacggga acagacgcgt cgccagccga
     2941 tacgagccgg acgccgaacc cggcctacgc gtccgacgct ctggagccgg acgattggcg
     3001 gaccattttc gacgtacccg aatcgccgtt gtggcgagcc gccttcgatg cggccaatgc
     3061 cggcgtcacc gtggtggccg ccaccggcaa ctacgccggg accgtgtaca ttccggcagc
     3121 cacgccaggg gtgatcgcgg caggtttcct caccgtcgag cgcctggtct cgaaccgcct
     3181 ggaggaggcc gcggccaagc cgccggcttt tattcaatcg cgctatgccg atctgcaact
     3241 gttgcagcca gccgacgtcc tcgggtccag cttcgcctcg ccgctgatcg ccgctttcgc
     3301 caccttgatg gccgatcgca aggagttgcc ggcctacgtc cggtgtgcgc gccgtgccgc
     3361 gaacgcgtcc ggtgcgctgc ggctgctcca ggaaggcgag tcagcgaaca gtgacttcct
     3421 gatgctcacc gactcgctgt tcaagggggc tctggcgagc gcaccgcaca cgcactatga
     3481 cgaggatgtg tattcgatca cgccttgtcc tgaatgcgcg gttttcgcca accccgcgta
     3541 caacgacttt gggctgttca ccatgtccac cggaaatgtg gacattgccg ttcggctctt
     3601 cgcctcgctt cgccagatcg ctcccaccaa tccctacgcg gccgcgaacc tgggggtggc
     3661 gttcgccatg ttggcgcagg ccgccaagga cgcgggcgat cttgagcgga cgcgcgtcct
     3721 gctcgaggcc gccgtggggc acgtgggaga cgcggtcggt ttgcgcccgg gctatgcgcc
     3781 ctatgccgca cggttggccg agtttgaagc ggcccgagtt gatccgacga aatggacgtt
     3841 ggccaggtag aaagcaacaa atcacaacga agggggacgg aaatggcagt cgaccgcatt
     3901 tctatcaaga tcgaagacaa tgacgacctg accgcggcgt acgccgctgc ttcggccacc
     3961 ggtgtccccg tccaggcggt caccccgccg cccagcggtg acctggaggc gcagatcgag
     4021 cctgtgaccg ctgtgctgat cggcgccggc gtggtcgccg cagtcaagct catcctcgat
     4081 tggtgggagc ggcgcaaggg agggctggtc atcgaccttc gcaaggacgc gaccgacatg
     4141 ttctatcgcg acaaggacct gacttatggc ttcgtggcca ccattcccga gaagggtgga
     4201 aaggtgaccg tggacgtgaa ggatctgccc gacgcggcaa cgacctggat caatctggtc
     4261 atcaccagcg cattcaagac cgtgcaggat gcggcgaagg cagccaaggc tgcggtcggt
     4321 gattcgaagg tgagcgtgga gcctgcggcg gccggctgac cagaccacga gcgaggtcat
     4381 gcctcgttcc acgggcggcc gcgcctaagc tcgagagtga cgaaggagcg gccaatgccg
     4441 aagcgaagat ggagcgacct ctcggcaggt acgcgggctg cgatcgttat tctcggcgcg
     4501 gccgacctgg cgctacgcgc ctggtcgatc gctgacctgt cccggcggcc gcaagcgcag
     4561 gtcaacgggc cgaagctgct atggatgacc accctctccg tggtgagctc gatgggcctg
     4621 ctgccggccg tttacctcag ctgggggcga cgcgcgggtg ctgcggggag tcggcggaaa
     4681 cgccgaacgc cttagcgacg ctgctcaact gggcagctgc aggtgaaggt cggcgtgagc
     4741 ccgtcggata tggctctcga cgagatcggc ggcggcctcg tcccggcctt cggccactgt
     4801 ctccagaatc tccaggtgct cggtcgccag ccggcgccga aggacctccc agtcggccac
     4861 ggtggtctgg gcgtcgcgga tcatcggcgc caccgcctgc cggatggcca cagtgaagtc
     4921 gctgatcagc cggttctggc ccacctgggc caaggccaca tggaaggcgg tgtccaggtc
     4981 gttgaactcc gccgatccgg tggccagggt catccgccag gccagcgtcc gagcctcctc
     5041 gagcgaatct ggcgtggccc gccgcgcggc ctctgagatc gccgcccgtt cgagcaccac
     5101 ccgggtgtcg gtgagttcgt cgacatcggt cgaacccagg gccagctgga ggctcagcat
     5161 ccggccgaac gcctcgctgg gcgtggtcgt gattcgggtc ccgtgcgcgc gtcccgctgc
     5221 cgtcgccgcg atgccctgag cttgcagtac cttgatcgcc tcgcgcaccg cgctgcggct
     5281 cacgtccaga tcagtggcca actgacgctc ggtgggcagc ttgctgcccg ccggcagttt
     5341 ccggtcgatg atcgcctggg tcaggtaggc catcaccgtt tcgaagccgc gcggctcagc
     5401 gttcaagctc tgctccgtcg ccggtgcggc gtcgtcctgg tggccggtca tgcagcccag
     5461 tctccaccaa tccggcgctt ccacgccccg cgccacgacg catggtggga ggggttgaca
     5521 tgacaaactc ccaggcttat ggtcagacca aagattaatg gtcggaccaa acatcgaatg
     5581 gtctgaccgg aaatcaggtc cctgagaaca gcggagtctc gatggtccct cttgaggcgt
     5641 tcctgcccga caccaacccc actggcatgg gctggctgtc cgctctgctc gcggcgctcc
     5701 cgatcgtggc catgctggtg acgctcggcg ccctgcgctg gaaggcccac ctcgccggag
     5761 cgttctcctg ggtggtggcc ttcgtcgtgg ccatcgccgc cttccggatg cccgccctga
     5821 tggcggtcag caccagtgtg gaaggcttcc tctacgggct gttcccgatc gtctggatcc
     5881 tcctcaccgc tatctggatg tatgacatca ccgtcgtctc gggccgcttc gacgacctca
     5941 agcggacgtt cttcctgctc agcgacgacc cccgcgtcct cgccctgctg atcgccttct
     6001 gcttcggcgg cctcctcgaa tccctggccg gcttcggtgc gccggtggcg atcaccgccg
     6061 cgatgctggt cgcgatcggg ttcagtccgc tgcgctcggc catgaccgcg ctactggcca
     6121 acacagtgcc cgtggccttc ggtgccgtcg gcctgccggt gatcatggcc gccaaggttg
     6181 gcgagttcga caacgtcctg ccgatctcca ccatctccgg acggatcacc gcgatcctat
     6241 gcctggtcgt cccgttcctg ctgctcgccg tgatcgacgg gaagcagggc atcaaggagg
     6301 tctggccgtt cggactggtc gtggcgacca gcttcggcgt caccaagtgg atcgtttcca
     6361 gcaccccgct gtacaacctc accgaggcgg tggctgctgt ggtgagcgtc gccgtggcga
     6421 tcgcgttcct gcgcttctgg aagcccaccg gcggagccgc cgccatcgac cgcgtggcgc
     6481 gtccgatcga ccccaggatc gagagccacc tacccgcccc cgcgccgacc gacccggacg
     6541 cccccgccct caccaggaac cgcatcctga tggcactcgt cccttacgcg ctggtgatcg
     6601 tggtgttcag catcgcggcc atcccgcagg tgaaggccgc gctggcaggc accgatctca
     6661 agctgcactg gccgctgctg gacgagatgc tgaacaccgc cggcgcgccc gcggcccacc
     6721 agacctacac tttcgcatgg gcatcgacgc ccggcatcct gctcgctctg gtcgcgatcc
     6781 tcaccgggct gatctaccgg atgggctgga agcggatctt caaggtactg gtcgacaaca
     6841 tggtcaaact tcggttcacg gcactcacca tcggctcggt ggttgccctc gcctacgtga
     6901 tgggcgactc cggacagacc atggcccttg gcctgttcat cgccagctct ggcgccctct
     6961 acccgttctt cgcgccggtc cttggctgga tcggcaccta cgtgaccggt tcggacacct
     7021 ccgccaacat cctgttctcc gggttgcagt ccggtgtggg gcaccagatc ggcggcgatg
     7081 gtgcccacct cgacgtcctc cagatgcgca cgctcctggt cgggggcggc ggcatcggtg
     7141 gggtcgtcgg caagatgatc tcgccgcagt cgctgtccgt cgcgtccacc gcgatcggcc
     7201 tgcccggttc ggagtccgcg atcctgcgcc gcatgatccc ctggagcctg gtcctgctgg
     7261 tcctgctctg cctcctctcc ggcctgatgt ccacgcccgc cctgagttgg ctcatcccct
     7321 gaaccgccga tagcaccacc aaggagtcgc acccatgtcc gtatccaccg gcaccgcagc
     7381 caaggtcggc aagcccgggc tcggcaagcg ggtggcactg ttcgccacct gcatcaacga
     7441 cgcgatgatg cccgccgcgc ccgaagcgac cgtccgcctc ctggagcggc tgggctgcac
     7501 cgtggagttc ccgcgacagc agacgtgctg cgggcagatc ctcaccaaca ccggctactt
     7561 cgacgaggcc gtcggcacgg tgaaggcgta tgtcggggcc ttcagcgact acgactacat
     7621 cgtcgggccg tctggttcgt gcgtcgcttc ggtgcgccac cagcacccga tgctggctcg
     7681 gcactccggt gactccggcc tcgaaaaggc ggccaccgca gccgcgtccc gcagctacga
     7741 actcaccgag ttcctggtgg acgtgctggg ggtggtcgac gtcggggcct acttcccgca
     7801 ccgggtcacc taccacccgt cctgccacgc gacccggatg gccaagatcg gcagccgtcc
     7861 ctaccagttg ctcgaagccg tccggggtct ggagctggtg cccctgccgc acgcggagga
     7921 gtgctgcggc ttcggcggca ccttctcact gaagaacccg gacatctcga tcgcgatggc
     7981 ctccgacaag gcccggcatg tgcgttcgac cgaggcggag tacctcgtcg ccgccgacaa
     8041 cgcctgcctg ctcaacatcg gcggggtgct gcatcggcag ttctccggaa tcaagaccat
     8101 ccacctggcc gagatactgg cccagaccga acaggatgca gcatgagcct ggacgcccag
     8161 cgccgcaccc ccgcgccgcc cccgggcgga tgggacggcc ttcccgtctt tcccaaggcc
     8221 gtcaaggtcg aactccagct caccgagcag cgcaagaacc tgcgtcacgc aaccggcacc
     8281 attcgggcca agcgggcgct tcgcgcgtcc gaagtgccga actgggagga actgcgcgac
     8341 gcagcggccg cgatcaagga ccgggtcgga cgccacctcg acagctacct cgagcaggcc
     8401 gaacgcgcca tgctcgacgc cggcatcacg gtgcactggg cccgcgacgc ggccgaagcc
     8461 aaccagatcg tggtcgacat cgccaaggcg aaaggggtcg acgaagtcgt caagatcaag
     8521 tcgatggtca cccaggagat cgacctgaac gagcacctgg aacgcagtgg aatcgcggcc
     8581 tgggaaaccg acctggccga gctgatcgtc cagttgggcc acgacctgcc cagccacatc
     8641 ctggtgcctg cgatccaccg gaaccgctcg gaggtgcggg agatcttcac cgaggagatg
     8701 ggacgctacg gcctggctgc gccttccggc atctccgacg acccggccga actcgccgcc
     8761 gcggcccgcg cgcacctgcg ggaaaagttc ctacgggcga agatggcggt ctccggcggc
     8821 aacttcatcg tcgccgagac cggcacgctg gtgatcgtcg aatccgaagg caacggacgc
     8881 atgtgcctga ccctgcccga gaccctggtg tcggtggtag gtatcgagaa gatcctgccg
     8941 acgctggccg atctcgaggt cttcctcaag ctgcttccgc gctcgtccac cggtgagcgg
     9001 atgaacccgt acacctcgtt ctggaccggg gtcaccaccg gcgacgggcc ccaggacctg
     9061 cacgtcatcc tcctcgacaa cggacgcagc cgggtgctcg ccgatccggt cggacgcgcg
     9121 gcgctgcgct gcatccggtg ctcggcgtgc ctcaacatct gcccggtcta cgaacgggtc
     9181 ggcgggcacg cctacggctc gccctaccca ggtccgatcg gcgccatcct gaacccgcaa
     9241 ctccacgggg tcagcgaccc caaggaccag gcgctgccgt tcgcgtccac tctttgcggg
     9301 gcctgcaacg aggtctgccc ggtgcggatc ccgttcaccg acatcctggt gcagcttcgc
     9361 cacaaggtgg tcgagtacaa gaccggcaag caccccacgg tcgagcaggg aatcatgaag
     9421 acggtctcct gggtcatgtc cgacggcaag cacctggaga aggccgaact cgccacccgt
     9481 gccgcgggca gggtgcttgg tgaccgcgtg ctcggcccga tgcccgtccc gctcgccgga
     9541 cgctggctac gcgcccgcga cgtggacgcg ccgccggtgg agacgttccg gcagtggtgg
     9601 aagcaccatc gcgctgccga cgggaaggag ttgtgatgaa tgcgcgcgaa gagatcctcg
     9661 gacgggtacg tgcggccacc caggacgtga ccgtggcgga cccagccctc gacgtcccga
     9721 tcgcgtggac ctatgggcag cccctggcca ccgcggacgt gctggctgac ttcgtggaga
     9781 aggtggagga ctaccgcgcc accgtggtgc gggtgccggc agtcgaagtg ccgcgggccg
     9841 tcgccgaggc actggtcacg ctcggtgcca ccagtgccgt gctgccctcc ggcgttcccg
     9901 cgacctgggc tcaggcggtc gcggaggcag gcgtccagct gttcgcggac gagccgcagc
     9961 tggactacgc gacgctcaac acggtgtcgg ccgtggtcac cgcggccgcg gtcggcatgg
    10021 cggattccgg cacgatcgcc ctcgaccacg gtgacgacca gggtcggcgc gtgttgagcc
    10081 tgctacctga catccacgtc tgcgtcgttc gggccgatca ggtcgtgagc gatgtgcccg
    10141 agggtgtggc ccgtctttcg ccgggattgc gcgcgggtcg gccggtgact tggctcagtg
    10201 gcgggtcggc gaccagcgac atcgagctga gccgcgtgga aggcgtccat ggtccccgga
    10261 cgctgtgggt ggtgctggct gagggctgag ccgactcgtc gtatggacat tcggaatcct
    10321 ctgcgcgccg acttccggcg tggttgattc tggctggtgc cggctcgatg ggggtctggc
    10381 ggggtcatgc catgggggga acggatggga tcttcgactg cgttgcggcg cttgctggcg
    10441 gtagtggtag cggtcttcct cacgctggcg gggctgctgg ccccggtgac gccggcgcag
    10501 gccgccacca agccgaaagt gaccggaatc tcgatcacgt ccggcaacct gcagggcggt
    10561 gagacgctca ccattcgggg cacgggcttc aagaaggtga agcgagtgct cttcggcacg
    10621 gtgcgggcga ccaacgtgaa ggtcaagtcc agcaagcgga tcacggtcac cgtgccgccg
    10681 ggtcaggccg gcgtagtcga cgtgcgtgtc gtgaccaaga agggcacctc caagcggacc
    10741 actcgcgcca agtaccgcta cctcgccgcc gagcctgctc ccgtcccaag cgcagcacca
    10801 acggtgtcgg gcatcgcacc tgccctgggc agcgtgtctg gcggcacggt ggtgaaactc
    10861 actgggtccc gcttcgccgg agccacccag gtgctcttcg gatcgcgccc gggaaccggt
    10921 ctggtggtcg cctccgactc ggcgatcacg gtgaccaccc catccggagt ccagggcgcg
    10981 gtcgtggtga aggtggtcgg tcccggtggc accagcgccg gaaccagctt cgtctatcaa
    11041 ctcgtcgaca ccatgtcagc cggccagcgg ctgtccgcgg ggcagagcct cagctccagc
    11101 aacggcggct accggctgac catgcaggcc gactgcaacc tggtcgtgta cacctcggct
    11161 ggagccgcga agtggggcag tagcacgaac cggaacgatg cgggctgtca cgccgacgtg
    11221 ctggcggacg gcaacctggt catctacacc tccggcggtc aggccgtctg ggcgagcaac
    11281 accggcggct tcaacggtgc cgcactgcgg atgcaggacg acggcaacct ggtgatctac
    11341 cacggctcgg ccgcggtctg gtccagcaag gggggatcgg gaaaggtctc cagcttcaaa
    11401 gcctgggcgc tgaactcggc caactggaac agcaccaccc gcatctacga cgccgccggc
    11461 aacatcgtgg gtggcggcaa gcctgggatc gacgcggacg gtgccttcgg cgctcagtgc
    11521 gccgacctgg gaatagcctg gtccggtcag gctggtcacc gggtcgggtt cgacggctgg
    11581 gacagcagca cggctggcaa ggcaggctgg cacttcgttt cgggcaacct gagtcaggtc
    11641 cagccgggtg acgtggttac ccgggtgggc ggcaagcagc atgtcgtggt cgtcaccggg
    11701 tccccgtccg gcggttcagt ggaagtgatc cagcagaacc ctggctcgcc ggccgtcgcc
    11761 agctactcga cctcgaccag tggggtgatc tggcggctca actgagctcg ccgcttcgaa
    11821 ccgcgggccg ccgcggcgct cgggttggcc gccgcatccg gatgcgggca cgcttgtccc
    11881 accatgactc ctgaaccgat cgagctcttc attcgcgcgg tcgacctcac cggcgtgttc
    11941 gcgaacgcga tcctcggcgg cctgatcgcc cgggccaagc gattcgaccc ggtcggcttc
    12001 ctcaccttgg cgatcacgtc cgggctcggc ggcggcatga tccgcgacgt gctgcttcag
    12061 gtcggcccgc cgctcgcgct caccgattcc gcctacctgc tcgtcgccat cgccggcacc
    12121 gcggtcgcct tcttcgtgcc ggtcagcaag cggctctggg atctcagttt cccctacctg
    12181 gacgcgctcg ccctcgggtg ctggtccgct gcgggtgcgt ccaaggcgct ggccgccggt
    12241 cttggctggt tgcccgccgt catgatgggc accatcacgg ccgtcggcgg gggagtgctt
    12301 cgcgacgtcg tgctgcgcaa gaccccgtcc gtcttcggcg gcaacactct gtacgccacc
    12361 tcggccatcc tcgcttcgat cgtgctggtc gtcctgcagc ccctcaccgt cgcgccgacc
    12421 gccaccctca ccgccatcgg caccggcatg ctggtcaccc tgctggcccg gcgcctcgaa
    12481 tggcgcctgc cgtcctccta ttcctggtca cccaagcggg ccgtcgccat gctgccgcgc
    12541 ccgcgctggc gtccaaagcg caagcggacg cggccgcgcg ccgacgaggc ataggtctcg
    12601 acaggctcga ccaactgcat gggctcccgc tggcagactg gcgtcgtgca cagccccagc
    12661 agttggcggg cgcggctcgc caagcgcatc gatccggcga ccgtccgcaa ccgggtcgag
    12721 aacgtcaacg acggcatcct cgccatcgcc ggcttcagcg aagggctggc cggtggcggc
    12781 gaaatccaga acctgtaatc gccgatcatc ctgctggccg ccttcgccgg cgcactatcg
    12841 gttgcccgcg tggtgttgag cgcgggcttg gctgaccggg acgccgagct cctcgtcgcc
    12901 gcgtaggaac agcgtcgcct ggcgctcggg cccactgaac agatcgccga aatcgccacc
    12961 tactacgagg gccgcggcgt caccccggcc attgcgcaac gggtcgctga ggagctgaac
    13021 gccgccgaca gcctcggcgc ccaaccccag atcgacggct tcggcaagcc gacggccgtg
    13081 tccggtgcga tcagtgtcgc gctcggcgcg ggggttgcct tcctgatcgg cgcgacggtg
    13141 cccatcctga tctccgtcat caccccggtc acctggcgcg acgagtacac catcctcgcc
    13201 gtggcggtcg cgttggggtt cacctcgatc atcctggccc gactgggcca cacccgcatc
    13261 tggcagcccc tggcgcggtc ggtcttcatc gggcttgccg cgttggggtc gagctatttc
    13321 gtcggctcga tgctgctgta actcaggtgg ggcagctacg aattgatcag cccgaggcaa
    13381 gtagcgcgtc gatccgcttc cggagcacct gctcaggctg cgcgccgacg aaccgcgcca
    13441 cattcttgcc gttccggagc atgaccaggg tcgggatgct gctcgcgtcg aacctggtag
    13501 cggtgcggcg gttgtcgtcc acgttcacct tgaccacctt cacccgtccg gcatagctgg
    13561 cggctagccg ctcgaggatc ggcgccacca tgcgacacgg gccgcaccac ggcgcccaga
    13621 ggtcgaccag cacgagctga tcggtatcaa tggcggacgc gaacccggca tcgctggcgt
    13681 ccaccaacca tggcagcgcc tggtggcagg aggcgcactg cggccggccc ttggcggcgt
    13741 ccggcacgcg gttcttcgtg ccgcaagccg ggcacacggc gacgcgagaa ctcatgaccc
    13801 gaacttagcg cgggtcgaga cacgccgttg gggtacgtgc gaaggatggt gagcgggtga
    13861 acccgccgtc gttgactgga ggctcaagtg acaacctag
//
LOCUS       DAOPRQ010000062        13664 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_3788_length_13664_cov_12.064443, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000062 DAOPRQ010000000
VERSION     DAOPRQ010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 13664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13664
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3788_length_13664_cov_12.064443"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            116..424
                     /gene="rplU"
                     /locus_tag="PKJ61_08845"
     CDS             116..424
                     /gene="rplU"
                     /locus_tag="PKJ61_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002513860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOC13969.1"
                     /translation="MYAIVRSGGRQHKVAVGDVLEIDKVEAKAGDSVALSPLLLVDGE
                     TITADAKKLGKASVTAEVIAETKGPKIRILKYKNKTGYKKRQGHRQRYTQVKVTGIES
                     "
     gene            434..694
                     /gene="rpmA"
                     /locus_tag="PKJ61_08850"
     CDS             434..694
                     /gene="rpmA"
                     /locus_tag="PKJ61_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017934149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOC13970.1"
                     /translation="MAHKKGASSSRNGRDSNSQRLGVKRFGGEQVNAGEIIVRQRGTH
                     FHPGEGVGRGGDDTLFALRAGKVEFGTRRGRRVVNIAIEAAE"
     gene            845..1621
                     /locus_tag="PKJ61_08855"
     CDS             845..1621
                     /locus_tag="PKJ61_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13971.1"
                     /translation="MRACWTRVVTLVAAAVAALLLTGCEIEGSVDVVSSEQVALDLTF
                     TGTAEDCQQFPDSSVYGLTVDTTYDDDQATCRMYGTVDARTLADVFPYFVATEVGDHY
                     LFSTRFGGYSFDSVDVTVGFPGDVVSATRGEVSGNQVRLTDPADFSGENAVVKIVAHS
                     RPGAQWWPALLAGGSVLAAGAAFVVGLLVGRRRRDASEVTELFDLTPDGERPIDRSWY
                     APNPQTRVPAEPAAGDFRAPSEPADHSAWAPPAEGESDSS"
     gene            1698..3197
                     /gene="obgE"
                     /locus_tag="PKJ61_08860"
     CDS             1698..3197
                     /gene="obgE"
                     /locus_tag="PKJ61_08860"
                     /inference="COORDINATES: protein motif:HMM:NF008954.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HOC13972.1"
                     /translation="MAIPSFIDQTVLTVAAGNGGHGCASIKREKFKPLGGPDGGNGGN
                     GGSVILRVDPNLTTLVEFHRQSIRRAANGQPGRGDFNNGANGDDVVLAVPDGTVVSDA
                     ETGAVLADLVGPGSEVVIAGGGRGGLGNAALASSSRKAPGFALLGEEGESRKITLELK
                     VVADIGLVGFPSAGKSSLIAAISRARPKIADYPFTTLIPNLGVVVAGATTFTVADVPG
                     LIEGASAGRGLGHEFLRHIERCQAIVHVIDCATYEPGRDPLTDLDVIENELSAYGGLA
                     DRPRMVALNKVDVPDAAELAELVTDDLKARGLPVFSISTKTGSGLQGLTYAMAELVAK
                     RRAEAPKPEPKKIVIKPAPVTTGPEFSISRQGDGEGGFVWRVRGDKPERWVRQTDFNN
                     PEAVGYLADRFARLGIEEELLAMGALAGDAVAIGAENPVVFDFAPQVEIGAEILSRRG
                     EDQRLITERPAAQRRRVKDAEYHAAKAAERAAEQYTRLASEGFEDAEDE"
     gene            3190..4335
                     /gene="proB"
                     /locus_tag="PKJ61_08865"
     CDS             3190..4335
                     /gene="proB"
                     /locus_tag="PKJ61_08865"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016456332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HOC13973.1"
                     /translation="MSEAAGSGLRPEIVDARRIVVKVGSSSLTDASGALDMMKLEALV
                     DALAATRARGQDVVLVSSGAMASGMGPLRMKHRPRDLASKQAAAAVGQSLLVGRYGTL
                     FARFGLTVGQVLLTAEDVARHDHYRNALRTFTRLIELGVVPIVNENDTVATHEFRFGD
                     NDRLAALVAHLVGADALVLLSDVDALYTAHPANPDARPIRLVTDVDELVVDTSGRGAE
                     IGTGGMATKLQAAQIASASGIPAVLASAEAVADALMGREVGTLFRPTGKRRSRKLMWL
                     AYASVSQGELVLDAGAVKAVTEKKASLLPAGIIEVRGSFVTGDPVRLVSESGTVVARG
                     LVAYDSADLPAIIGKSTRDLVAELGPQFDREVVHRDDLIVRHRKCKP"
     gene            4332..4991
                     /locus_tag="PKJ61_08870"
     CDS             4332..4991
                     /locus_tag="PKJ61_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13974.1"
                     /translation="MTPVELIGWAAAVVGTLLGVPQVYRLARTRTVAGLSMPAWQAIL
                     ALNIAWTSHGIILAKANMIVPNVLGLASTLPVLVMMARELGRPLGRVLLPGVLVAAMM
                     IGVDLAFGTAVYGVVAIFPALFANLGQCVELVRSPSVAGVSPVFLIGGVVNQVLWLAW
                     GLLVEDAGTIITACVTLAVTSFNLVWWILRRLGLRSFGVPTRDEVRAYVRARARAGTP
                     S"
     gene            5115..6356
                     /locus_tag="PKJ61_08875"
     CDS             5115..6356
                     /locus_tag="PKJ61_08875"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HOC13975.1"
                     /translation="MTFADLAALSRVGARRLATLNRTQKDAGLQSMADALAAASDAVL
                     AANAEDVAIARAAGTPESLIDRLALNPGRIDSMVAGLRALAALPDPVGDVVRGWTNAN
                     GVRVRQVRVPFGVVGIIYEARPNVTADAAGICLKSGNAALLRGSSSALNSNRAVVAAL
                     RAGLAASGLPADAVALVEGDRSVTGEMMTARGLVDLLIPRGGAGLINAVVEGSTVPVI
                     ETGTGNCHLFVDASADQDKALQIMLNAKVQRPSVCNALETLLVHVDAAPTFLPKALAA
                     LAEAGVTVHGTEAVRGYSDAVVPAAADEFDAEYLTLDLACDVVDSIDAAIEHIRTHTT
                     GHSETIVTEDRRSADRFAAEVDAAAVLVNASSRFVDGGEFGFGAEIGISTQKLHARGP
                     MGLAEMTSSKYIVDGDGQTRS"
     gene            complement(6556..7002)
                     /gene="rnhA"
                     /locus_tag="PKJ61_08880"
     CDS             complement(6556..7002)
                     /gene="rnhA"
                     /locus_tag="PKJ61_08880"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017935997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HOC13976.1"
                     /translation="MPDQTTLHMWTDGACKGNPGPGGWGVLMTYGEHVRELFGGEALT
                     TNNRMELMAVIRGLESLKRHSVVHLHVDSVYVMNGMTRWIHAWKRNGWRTADKKPVKN
                     AELWQELETQVAKHEVHWEWVKGHAGDPGNERADQLANLGVDQVRG"
     gene            7099..7230
                     /locus_tag="PKJ61_08885"
     CDS             7099..7230
                     /locus_tag="PKJ61_08885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13977.1"
                     /translation="MQILEAVVIDVAAPVVVGLIAFGILAAGLAFVRGVGKGRPHAK"
     gene            7227..7874
                     /gene="nadD"
                     /locus_tag="PKJ61_08890"
     CDS             7227..7874
                     /gene="nadD"
                     /locus_tag="PKJ61_08890"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HOC13978.1"
                     /translation="MSETPIAWEAQGTRKQRLGIMGGTFDPIHHGHLVAASEVQARFD
                     LDEVVFVPTGQPWQKADRQVSQAEDRYLMTTIATASNPRFSVSRVDIERDGLTYTVDT
                     LRDMRSLRGSEVDMFFITGADALSQILTWKGVDELWDYAHFVGVSRPGVPLGESDIAH
                     LPDDKVTLLEVPALSISSTACRDRVRDGLPIWYLVPDGIVQYIAKRGLYRNQGIK"
     gene            7871..8272
                     /gene="rsfS"
                     /locus_tag="PKJ61_08895"
     CDS             7871..8272
                     /gene="rsfS"
                     /locus_tag="PKJ61_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016667861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOC13979.1"
                     /translation="MTATEQALEIARVAARAAVAKLASDPVAFDVSERLAITDIFLIV
                     AATNERQVGAVVDAIEEALLKTGTKAVRREGDRENRWVLLDFLDVVVHVQHTEERALY
                     SLERLWKDCPRLDLGELETVPESSEDAVAGE"
     gene            8269..8880
                     /locus_tag="PKJ61_08900"
     CDS             8269..8880
                     /locus_tag="PKJ61_08900"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOC13980.1"
                     /translation="MTAFVLLRHGVTDWNDGGRFQGHADISLNDTGRTQAAAAAVALA
                     DAGIRRVYCSDLSRAAETAAIVAARLGLEVRTDARLREVDVGSWAGLSMAEAGELEPD
                     FWPALREGRDFRRSPSGETATESGVRVAGALLDYAAPADADADAEDVVLVVGHGLSLR
                     VAAMLLLGLDYSHARRFGGLRNCHWMVLQSGEEHWRLLSYNVG"
     gene            complement(8963..10228)
                     /locus_tag="PKJ61_08905"
     CDS             complement(8963..10228)
                     /locus_tag="PKJ61_08905"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HOC13981.1"
                     /translation="MPALARLVRALAVALTAVALVVGVGTSAPAKTVGTNNQPITNRA
                     GVTFGVLSHRGGALQWPENSVEAFENSVAAGFDSIETDILFTADGQAVMSHYDVLPAR
                     CTNAGESIHLMTSAQVAEVRCADLSGQKVVPLATFAQLADALAKNPQVGLTLDIKSYV
                     GQPAAEQRAYAEQAIQQVQDHGLLGRTSIISFYWKNHLPVIRKAAPKIYVLALDNGTM
                     DLDRVRLADKLKADGYGIKMKYTSEYLVKYTKAKGMDAVPWEVLGDEQRAFGIYYGGK
                     LQLFSSDEPTTTRTQLVAGQINLNPVPTPTLTSLGTPVLLSQAKYVKNKRSYPLVSGT
                     AVPKEDVAQLRSVTLSITVTGGKGTGKLYVGGMSTPLKSSVSKKLPKGTSTFTISAPL
                     GDKGKLRIYTTKTVTLTVTAVAYTRVRFA"
     gene            complement(10342..10992)
                     /locus_tag="PKJ61_08910"
     CDS             complement(10342..10992)
                     /locus_tag="PKJ61_08910"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOC13982.1"
                     /translation="MSRPASGRIRVLIVDDDPFVRQALTQFVNTSPDLTVVGSAGDGA
                     AAVKLVDKLKPAVVLMDVQMPDVDGIAATHALRAAHPEVRVLMLTAFAGHDTVDLAVR
                     AGASGFLQKTAKPAEVVNAIRLVARGHGVLPVTELDRRRADATPAPGEAVDLTRRERE
                     VLAALIRGLSNREIAAELQLAESTVKLHLSTLMAKLGVTSRVQALVRAHQLGLRAQ"
     gene            complement(10989..12119)
                     /locus_tag="PKJ61_08915"
     CDS             complement(10989..12119)
                     /locus_tag="PKJ61_08915"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOC13983.1"
                     /translation="MASPTRTGSLLQSAVRRGYLEPVLVGLLAVFVIASHLLVTMHAG
                     ALLYDLTALAAAALTGRWPRAAGIAFAAVMAANLLFIDWPTVSVYAALLVLFCFGVRG
                     RQRERLWFTGLYLPLLWLLNWLGLPPGVSVVPYLFIWIVFVAVAWLAGSAWHGLIVAA
                     EESRLRAALEERHELARELHDSVANSLAAVSLRAEQARLKGHADDAELSAIADEAARA
                     VGEFGQIVQVLRRDSGEPEPSTGSVSLEGIIDNAVQRLRSRGFAPNLSWDGPRPSLPV
                     KAAHTLARVIEEATNNMLRYGDPGADSAIVVEVSPGTAEFAFINRPKNARGAGGSGFG
                     LTSMSERVSALGGSFTSSQVGESWLTSFQVPIAALPAAAVPS"
     gene            12364..12504
                     /locus_tag="PKJ61_08920"
     CDS             12364..12504
                     /locus_tag="PKJ61_08920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13984.1"
                     /translation="MRALAALVLLFPVCSGQVIASAPTVEVTVAVAGICDVFPYLPGC
                     PR"
     gene            12550..12822
                     /locus_tag="PKJ61_08925"
     CDS             12550..12822
                     /locus_tag="PKJ61_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13985.1"
                     /translation="MITAESQRTFAAKLEAARVAQHLSIRTVGRIAKAPPTTVHGWLT
                     GTHFPALTMRGNYLRMVETLGLMAEMPADIREELWIVDESRAKAAG"
     gene            complement(12902..13423)
                     /locus_tag="PKJ61_08930"
     CDS             complement(12902..13423)
                     /locus_tag="PKJ61_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13986.1"
                     /translation="MSAFWEGVASNVVGTFVGAGLALLTSFFVIRHSETRNDLRLLQA
                     LIDRLYRSRALRAGQLATAFDTPDARENERRSTKSVLATRDRIAFTSDSLSGHSDAFD
                     ELDRMHVACLRYLNDVQDDPSGYIAGLMMLRSQLEPEVTALCERYRTLHPREIGAADL
                     RGRITGHSESNDS"
BASE COUNT         2148 a         4473 c         4766 g         2277 t
ORIGIN      
        1 tcttgaccct cggtgctcgt tgtgctggcc ccgtgccgcc agagcacaca atcaggcagc
       61 gttccactcc tgtggattgc gccattcggt gaagaacgta ggaagtaggt cgggcgtgta
      121 cgcgatcgtg cgcagtggtg gccgtcagca caaggttgct gtcggtgacg tcctggagat
      181 cgacaaggtc gaggccaagg ctggcgacag cgttgcgctg tccccgctgc tgctggtcga
      241 cggcgagacg atcaccgcgg atgccaagaa gctgggcaag gcgagtgtca ccgccgaggt
      301 gatcgccgag accaagggtc cgaagatccg cattcttaag tacaagaaca agaccggcta
      361 caagaagcgc cagggtcacc gccagcggta cacccaggtc aaggtcaccg ggatcgagag
      421 ctgaggcact gacatggcac ataagaaggg cgcatcctct tcccgtaacg gtcgcgactc
      481 caactcgcag cggctgggtg tgaagcgttt cggcggcgag caggtcaatg ccggcgagat
      541 catcgtccgc cagcgtggca cccacttcca ccccggtgag ggcgtgggtc gcggcggtga
      601 cgacaccctg ttcgcgttgc gtgcgggcaa ggtcgagttc ggcacccggc gcggacgccg
      661 cgtggtgaac atcgccatcg aggccgctga gtaactccag caacttctcc gagcaggacg
      721 gtccgtacgg gccgtcctgt tctgcgtttg cgtcccgttg tcgctgactg tggcttcatg
      781 caccttctgc ggtgcgcaga tcgacagtga agcgcgctac gagtagggcg ttaagctcgg
      841 ctccatgcgc gcatgctgga cgagagtggt gaccctggtc gccgcggcag tggctgcgct
      901 gctgcttacc ggttgcgaga tcgagggcag cgtcgacgtg gtttccagcg agcaggtggc
      961 gctcgacctg accttcaccg gcaccgcgga ggactgccaa cagttccccg actccagtgt
     1021 ctacgggttg accgtagaca ccacctacga cgacgaccag gcgacctgcc ggatgtacgg
     1081 gacggtggac gccagaaccc tggctgacgt gttcccgtac ttcgtggcca ccgaggtcgg
     1141 cgaccactac ctgttcagca ctcgatttgg gggctatagc ttcgattctg tggacgtgac
     1201 ggtgggcttc cccggcgatg tggtctcggc gacccggggc gaggtctcgg gcaaccaggt
     1261 aaggctgacc gaccccgccg acttcagcgg ggagaacgcg gtcgtgaaga tcgtcgccca
     1321 ctcccggccg ggagcgcagt ggtggcccgc gctgctggcc ggtggcagtg tgctcgcggc
     1381 cggtgccgcc ttcgtggtcg gcctgctggt cggacgccgg cggcgggacg cgtccgaggt
     1441 aacggagctg ttcgacctca ccccggacgg cgaacggccg atcgaccgct cctggtacgc
     1501 gcccaacccc cagacacggg tgcccgccga acctgctgcg ggcgacttcc gggcaccgag
     1561 cgagcctgcc gaccactccg cctgggcgcc gccggccgag ggtgaatccg attcgtcgta
     1621 gcgggccgat agaatccttc tcatcggtgc cggccgttgg ctgcactccg atctcctagc
     1681 gcacaaggtt cgaagctgtg gccattccct ctttcatcga ccagacggtg ctcaccgtcg
     1741 ccgccggcaa cggcgggcac gggtgcgcgt ccatcaaacg cgagaagttc aagcccctcg
     1801 gtggcccgga cggcggcaac ggcggcaacg gcggatcggt gatcctgcgg gtcgatccga
     1861 acctgaccac gctggtcgag ttccaccgcc agtcgatccg ccgcgcagcc aatggtcagc
     1921 cgggtcgtgg cgacttcaac aacggcgcca acggcgatga cgtcgtgctg gccgttcccg
     1981 acggcacggt ggtctccgat gccgagaccg gcgccgtgct cgccgacctc gtcggcccgg
     2041 gttcggaggt ggtcatcgcc ggcggcggac gcggcgggct cggtaatgcc gcgctcgcgt
     2101 ccagttcgcg caaggcgccc ggtttcgccc tgctcggcga ggagggggag tcgcgcaaga
     2161 tcaccctcga actgaaggtg gtcgccgaca tcggcctggt cggcttcccg agcgcgggca
     2221 agtcatcgct gatcgcggcg atctcccggg cccggccgaa gatcgccgac tacccgttca
     2281 ccaccctgat tcccaacctc ggtgtggtgg tggccggtgc cacgactttc accgtggccg
     2341 acgtgcccgg gctgatcgag ggcgccagcg cgggccgcgg cctcgggcac gagttcctgc
     2401 ggcacatcga gcgttgccag gcgatcgtcc acgtgatcga ctgcgccacc tatgagcccg
     2461 gccgtgaccc gctcaccgac ctcgacgtga tcgagaacga gttgagtgcc tacggtgggc
     2521 tggccgaccg gccccggatg gtcgcactga acaaggtgga cgtgcccgac gccgccgaac
     2581 tcgccgagct ggtgaccgac gatctcaagg ctcgcgggct gccggtgttc agcatctcga
     2641 ccaagaccgg cagcggtcta cagggcctca cctacgccat ggccgaactt gtcgccaagc
     2701 gcagggcgga ggcacccaag cccgagccga agaaaatcgt gatcaagccc gctccggtaa
     2761 ccaccggccc ggagttcagc atctctcgcc aaggcgacgg cgagggtggt ttcgtctggc
     2821 gggtgcgcgg tgacaagccc gaacgctggg tgcgccagac cgacttcaac aaccctgaag
     2881 ccgtcggcta cctcgccgac cggttcgcgc ggttgggcat tgaggaggaa ctgctcgcca
     2941 tgggggccct cgccggggac gccgtggcga tcggcgccga aaacccggtg gtgttcgact
     3001 tcgcgccgca ggtggagatc ggtgccgaga tcctgtcccg gcgcggcgag gaccagcggc
     3061 tgatcaccga gcggccggcc gcccaacggc gtcgggtgaa ggacgccgag taccacgcgg
     3121 cgaaggccgc cgagcgtgcg gccgagcagt acacccgact ggcgtccgaa gggttcgagg
     3181 acgccgaaga tgagtgaagc cgctggctcc gggctgcggc cggagatcgt tgacgcgcgc
     3241 cggatcgtgg tcaaggtggg ctcgtcgtcg ctgacggacg ccagcggcgc gctggacatg
     3301 atgaagctcg aagcgctggt ggacgcgctg gccgccacgc gcgcccgtgg ccaggacgtg
     3361 gtgttggtgt cgtcgggtgc gatggcgtcc ggcatgggtc cgttgcggat gaagcaccgc
     3421 ccacgggacc tggccagcaa gcaggctgcg gcagccgtgg gccagagcct gctggttggc
     3481 cgctacggca ccctgttcgc ccgtttcggt ctcacggtgg gccaggtgct gctgaccgct
     3541 gaggacgtgg ctcgccacga ccactaccgc aacgcgctgc gcaccttcac ccggttgatc
     3601 gaactcggcg tcgtgccgat cgtcaacgag aacgacacgg tcgccaccca cgagttccgg
     3661 ttcggcgaca acgaccggct cgcggcgctg gtcgcgcacc tggtcggcgc ggacgcgctg
     3721 gtgctgctca gcgatgtcga cgcgctctac accgcgcacc ccgccaaccc ggacgcgcgc
     3781 ccgatccggc tggtgaccga tgtcgacgag ctggtcgtgg atacctccgg ccgcggcgca
     3841 gaaatcggca ccggcggcat ggcgaccaag ctgcaggcgg cccagatcgc gtccgcgtcc
     3901 ggcattccgg cagtgctcgc gtccgccgaa gcggttgcgg acgcactcat gggacgcgag
     3961 gtcggcaccc tgttccggcc aaccgggaag cgccgctcac gcaagctgat gtggctggcg
     4021 tacgcgtccg tttcgcaagg ggagctggtg ctggatgccg gtgcggtgaa ggcggtcacg
     4081 gaaaagaagg cctcgctgct gcccgccggc atcatcgagg tgcgggggag tttcgtcacc
     4141 ggtgacccgg tgcggctggt gagcgagtcg ggcacagtgg tggcgcgcgg cctggtggcc
     4201 tacgactcgg ccgacctgcc ggcgatcatc ggcaagagca cccgggacct ggtggccgaa
     4261 cttgggccgc aattcgaccg cgaggtcgtg catcgcgacg acctgatcgt gcgtcaccga
     4321 aagtgcaagc catgaccccg gtcgaactga tcggctgggc ggcggctgtg gtcggcaccc
     4381 tgctcggagt gccgcaggtg tatcggctcg cccgcacccg cacggtcgct ggactgtcga
     4441 tgccggcctg gcaggcgatc cttgccctca acatcgcctg gacttcgcac ggcatcattc
     4501 tggcgaaggc caacatgatc gtgccgaacg tgctcggcct ggcctcgacg ctgccggtcc
     4561 tggtgatgat ggcgcgcgaa ctgggccgtc cgctgggccg ggtgctgctg cccggcgtgc
     4621 tggtggccgc gatgatgatc ggcgtcgatc tggcctttgg gactgcggtc tacggcgtgg
     4681 tcgccatctt ccccgcgctg ttcgcgaacc tcggccagtg cgttgagttg gttcgctctc
     4741 ccagtgtggc cggggtatcg ccggtcttcc tgatcggcgg tgtcgtcaac caggtgctgt
     4801 ggttggcctg gggactgctg gtcgaggacg ccggcacgat catcaccgcc tgcgtcacgc
     4861 tcgcggtcac gagcttcaac ctggtctggt ggatcctgcg tcgcctcggg ctgcgttcgt
     4921 tcggggtgcc gacgcgcgat gaggtgcgcg cctatgtccg cgcacgggcc cgcgccggga
     4981 cgcccagctg acccgttgca tgtcggctga gccttgctgc gagtctgctg aacaatgctg
     5041 ttccgggcga ggacgtgctg ggcgggattg gaccaccgaa ggccgcccaa gcgctggcgc
     5101 gtaccctatg ccgcatgact ttcgctgacc tggccgccct ctcgcgagtg ggcgcgcgcc
     5161 gcctggctac gctgaaccgc acccagaaag acgcgggcct gcagtcgatg gccgacgcgc
     5221 tcgccgccgc ctcggacgcg gtgctggccg ccaacgccga agacgtcgcg atcgcacgtg
     5281 ccgccggtac gccggaatcg ctgatcgacc ggctcgcact gaacccaggc cggatcgact
     5341 cgatggttgc cggcctgcgg gcgctggctg cgttgcccga tccggtgggt gacgtggtgc
     5401 gcggctggac gaatgccaac ggcgtccggg tgcgtcaggt gcgggtgccg ttcggggtgg
     5461 tcggcatcat ctacgaggct cgcccaaacg tgactgcgga cgcggccggc atctgcctga
     5521 agtcgggcaa cgccgcgctg ctgcgcgggt cctcgtccgc gttgaactcg aatcgcgcgg
     5581 tggtggccgc gctgcgggcc gggctggcgg cgtccgggct gccggcggac gcggtcgcgc
     5641 tggtcgaggg ggaccgcagc gttaccggcg agatgatgac cgcccgcggc ctggtcgacc
     5701 tgctgatccc gcggggcggg gccgggctga tcaacgcggt agtcgaaggc tcgaccgtcc
     5761 cggtgatcga gaccggcacc gggaactgcc atctgttcgt ggacgcgtcc gccgaccagg
     5821 acaaggcgct gcagatcatg ctgaacgcga aggtgcagcg accgagcgtc tgcaacgctc
     5881 tagagacact gctggtgcac gtcgacgccg cacccacgtt cctgccaaag gcgctcgccg
     5941 cgctggccga agcgggcgtg accgtccatg gcaccgaagc ggtgcggggc tactcggacg
     6001 cggtggtgcc ggcggcagcg gacgagttcg acgccgagta cctgaccctc gacctggcct
     6061 gcgatgtggt tgattccatc gacgcggcga tcgagcacat ccgcacgcac accaccggcc
     6121 attcggagac gatcgtcacc gaggaccggc gctcagccga ccgcttcgcc gcagaggtgg
     6181 acgcggccgc cgtgctggtg aacgcctcct cgcgcttcgt ggacggcggg gagttcggtt
     6241 tcggcgccga gatcggcatc tccacccaga agctgcacgc ccgcggcccg atgggcctgg
     6301 ccgagatgac gtccagcaag tacatcgtgg acggggacgg ccagactcgg agctgaaccc
     6361 ggttttggtg gcatttggcg atgggccgat ccatcacagc gaatggaacc cgactttgat
     6421 ggcttttcgg gctaccaggt ggcgtcatat gccatcgaat tcgggtcacg atccggaggc
     6481 aggtcgcgac ggtgctgata agccgtccta atcgggtttg gtgagcggtg cacgatcacg
     6541 accgtgacga cgggcttagc cgcgaacctg gtcgacgcca aggttggcca gttggtcggc
     6601 ccgctcgttg cccgggtcac cggcgtggcc cttcacccac tcccagtgca cttcgtgctt
     6661 ggcgacctgg gtctcgagtt cctgccagag ttcggcgttc ttcaccggct tcttgtccgc
     6721 ggtgcgccag ccgttgcgct tccaggcgtg gatccagcgg gtcattccgt tcatcacgta
     6781 gacggaatcg acgtgcaggt gcaccacgct gtgccgtttc agcgattcga ggccgcggat
     6841 caccgccatc agttccatcc ggttgttggt ggtgagcgcc tcgccgccga acagttctct
     6901 cacatgctcg ccgtaggtca tcagcacccc ccaaccgccg gggccgggat tgcccttgca
     6961 ggcgccatcg gtccacatgt ggagggtggt ctggtcgggc atctggctcc tcgatcgtcg
     7021 aatgtcccgg attattccat ctcgcggcgc ccggcccggg acagcctgcc gagcagcgcc
     7081 ccgtgtagta tccgcggcat gcagatcctt gaggcagtcg tgatcgacgt cgccgctccg
     7141 gtcgtggtcg gcctgatcgc gttcggcatc ctggccgcgg gcctggcctt cgtccgcggc
     7201 gtcggcaagg gccggccgca cgccaaatga gtgagacccc gatcgcctgg gaggctcagg
     7261 gcacccgtaa gcagcgcctg ggcatcatgg gcggcacctt cgacccgatc caccatggcc
     7321 acctggttgc agcgagcgag gtacaggccc gcttcgacct tgacgaggtg gtgttcgtgc
     7381 ccaccggtca gccgtggcag aaggcggatc gccaggtttc tcaagccgaa gatcgctatc
     7441 tgatgaccac gatcgccacc gcctcgaacc cgaggttctc ggtgagccgg gtcgatatcg
     7501 agcgggacgg tctgacctac acggtggaca ccctgcgtga catgcgctcg ctgcgaggta
     7561 gcgaggtgga catgttcttc atcaccggtg cggacgcgct gagccagatc ctcacctgga
     7621 agggtgtcga cgagctctgg gactacgccc attttgtcgg cgtgtcccgt ccgggggtgc
     7681 cgctgggaga gagcgatatc gcccacctgc ccgacgacaa ggtcaccctg cttgaggttc
     7741 cggccctttc gatctcgtcc accgcctgtc gcgaccgggt tcgcgacggc ctgccgatct
     7801 ggtacctggt gccagacggc atcgtccagt acatcgccaa gcgcggcctc taccgcaacc
     7861 aaggaatcaa gtgactgcaa ccgaacaagc actcgagatc gcccgggtgg ccgcgcgcgc
     7921 ggctgtcgcc aagctcgcgt ccgatccggt ggcgttcgac gtctccgagc gtctcgccat
     7981 caccgacatc ttcctgatcg tcgctgccac caacgagcgc caggtcggcg cggtcgtgga
     8041 cgcgatcgag gaagcgctgc tgaagaccgg gacgaaggcc gtccggcgcg agggcgaccg
     8101 cgagaaccgc tgggtgctgc tcgatttcct cgacgtcgtc gtgcacgtgc agcacaccga
     8161 ggagcgggcg ttgtacagcc tggagcggct gtggaaggac tgcccgcgac tcgacctcgg
     8221 cgagttggag accgtgccgg aatcgagcga ggacgcagtc gcgggggaat gaccgcgttc
     8281 gtcctactgc ggcacggcgt caccgactgg aacgacggcg gacgcttcca ggggcacgcc
     8341 gacatctcgc tgaacgacac cggtcggacg caggccgccg cggcagccgt tgcgctggcc
     8401 gatgccggca tccggcgggt gtactgctcc gacctgagcc gcgcggccga gaccgccgca
     8461 atcgtggccg cccgattggg gctcgaggta cggacggacg cgcggctgcg cgaggtggac
     8521 gtcggcagct gggccgggct gagcatggcc gaggcggggg agttggagcc cgacttctgg
     8581 ccggcgctac gcgaaggccg ggatttccgt cgctcgccgt ccggggagac cgccaccgag
     8641 tcaggcgtgc gcgtagccgg tgccctgttg gactacgccg cgccagcgga cgcggacgcg
     8701 gacgcggagg atgtcgtgct ggtggtcggc cacggactgt cgctgcgcgt cgccgcgatg
     8761 ctgctcctcg ggctcgacta ctcccacgct cgccggttcg gcgggctgcg gaactgccac
     8821 tggatggttc tgcagtccgg tgaagagcac tggcgcctgc tcagctacaa cgtcggctga
     8881 ggtccccgga ggctttccag ccgtttgggg tacggctgat cgagcttgtc atcgcactag
     8941 ctggaccagc cgtccgccgg cctcaggcga accgcacccg ggtgtaggca accgcggtga
     9001 cggtgagggt gacggtcttg gtggtgtaga tgcgcagctt gcccttgtcg cccagcgggg
     9061 cgctgatcgt gaaggtgctg gtgcccttgg gcagcttctt cgagaccgaa ctcttcagcg
     9121 gggtggacat cccgccgacg tacagcttcc ccgtaccctt accgccggtc acggtgatcg
     9181 agagagtgac gctgcgaagc tgagcgacat cctctttcgg cacggcggtg ccgctgacca
     9241 gcggatagct ccgcttgttc ttgacgtact tggcctggct gagcaggacc ggagtcccca
     9301 gcgaggtgag ggtcggtgtc gggacggggt tcagattgat ctggccggcc accagttgcg
     9361 tcctggtcgt ggtgggctcg tcggagctga acaactgcag cttgccgccg tagtagatgc
     9421 cgaacgctcg ctgctcgtcg ccgagcacct cccacggcac agcgtccatg cctttggctt
     9481 tggtgtactt caccaggtat tcggaggtgt acttcatctt gatgccgtag ccatcggcct
     9541 tcagcttgtc cgcaagccgg acgcgatcca ggtccatggt gccgttgtcg agggcgagca
     9601 cgtagatctt cggagcggcc ttccggatga ccggcagatg gttcttccag tagaagctga
     9661 tgatgctcgt cctgcccagc aggccgtggt cctgcacctg ctggatcgcc tgttcggcgt
     9721 aggcccgttg ctcggctgca ggctgtccga cgtagctctt gatgtccagg gtcagcccga
     9781 cctgtgggtt cttggccagc gcgtccgcca gctgggcgaa ggtggccagc ggcacgacct
     9841 tctggccgct caggtctgcg cagcgcacct cggcaacctg cgcggacgtc atcaggtgaa
     9901 tgctctcacc ggcgttggtg cagcgcgcgg gcagcacgtc gtagtgactc atcacggctt
     9961 ggccgtccgc ggtgaacaga atgtcggtct cgatcgagtc gaagccggcc gcgacgctgt
    10021 tctcgaacgc ctcaaccgag ttctccggcc actgcagcgc accgccgcgg tgggaaagga
    10081 cgccgaacgt gacgcctgct cgattggtga tcggctggtt gttcgtgcct acggtcttgg
    10141 ccggagcgga cgtacccacg cccacgacca gcgcgacggc ggtcagggcg acggcaaggg
    10201 ctcgaacgag ccgggcgaga gctggcatgg cggcaccttt ggggttgggt ttccttcacc
    10261 ataggtcgcc aagatgagag cgcaaagcgc aaacccccta ctcaacgccc aaagctggac
    10321 ccgagctgta gccctggtcg gctactgggc acgcaggccg agctggtgcg cccgcaccag
    10381 cgcctgcacc cggctggtca cgcccagctt tgccatcagg gtcgacaggt gcagcttcac
    10441 cgtggactcg gccagctgta gctcggcggc gatctcgcgg ttcgacagcc cccggatgag
    10501 cgcggcgagt acctcgcgct ccctgcgggt caggtcgacg gcctcgccag gggctggcgt
    10561 agcgtcagcc cgacgacggt cgagttcggt caccggcagg acgccgtgcc cgcgggcaac
    10621 caatcggatc gcgttcacca cttcggccgg cttggcggtc ttctgcagga agccggacgc
    10681 gcccgcgcgc acggccaggt cgacagtgtc gtgtccggcg aaagcggtca gcatcagtac
    10741 ccgcacctct ggatgcgccg cgcgcagcgc gtgggtcgcg gcgatcccgt ccacgtccgg
    10801 catctgcaca tccatgagga cgacggccgg cttgagcttg tcgaccagct tgaccgccgc
    10861 cgccccgtcc ccggcggagc cgacgacggt caggtcggga gaggtgttca cgaactgggt
    10921 gagcgcctga cgaacgaatg gatcgtcgtc gacgatcagc actcggatgc gtccggacgc
    10981 gggtcggctc acgacggcac cgcggcggcg ggcagggcag cgatgggcac ctggaagctc
    11041 gtcagccacg actcgcccac ctgggaactg gtgaaagacc cgcccagggc ggatacccgt
    11101 tcggacatgc tggtgaggcc gaagcccgag cccccggcac cgcgagcgtt cttcgggcgg
    11161 ttgatgaacg cgaactcagc cgtgcctggg gagacctcga cgacgatcgc agaatccgca
    11221 cccggatcgc cgtagcgaag catgttgttg gtggcctcct cgatcacccg agcgagggtg
    11281 tgtgcagcct tcaccggcag actgggcctg gggccgtccc agctgaggtt cggcgcgaac
    11341 ccacggctac gcagccgctg gactgcgttg tcgatgatcc cctccaggga caccgagccg
    11401 gtagacggct cgggctcgcc ggaatcccgg cgcaggacct gcacgatctg gccgaactcg
    11461 ccgaccgcac gggcggcttc gtcggcgatc gcgctgagtt cggcgtcgtc ggcgtggccc
    11521 ttcagcctgg cctgttcagc ccgcagcgag actgcggcca gcgagttcgc caccgagtcg
    11581 tggagttcgc gggcgagctc gtgacgctcc tcaagggccg ctcgcagccg agactcctca
    11641 gcggcaacga tcagcccatg ccaggcggag ccggccagcc aggcgacagc gacgaacacg
    11701 atccagatga acagatacgg cacgacgctc acgcctggcg gcaggcccag ccagttgagg
    11761 agccagagca gcggcaggta gagcccggtg aaccagagcc gctcgcgctg gcgtccgcgg
    11821 acgccgaagc agaacagcac aagcagcgcg gcgtacaccg acacggtggg ccagtcgatg
    11881 aagagcaggt tggctgccat cacggcggcg aacgcgatcc cggccgctct tggccaccgt
    11941 ccggtcagcg cggccgctgc cagggctgtg aggtcgtaga gcagagcacc ggcatgcatg
    12001 gtgaccagca gatgggaggc gatcacgaac accgccagca gtccgacgag gaccggttcc
    12061 agatagcccc tccgcacagc cgactggagc aggctgcccg tccgggtagg tgaggccatt
    12121 ggtcgccctt cggagttgtt tccgctgacg gggtgcaggc agcgggccta gcttggagcg
    12181 catctgaagg tccttcgtca gaccgcgctc cgggcaagtc tggccatcat tctgccgacc
    12241 tgtacctaag tccagttcat ggtcgttgct gactggacga acgtgcagtg tttagggggc
    12301 tggacaggtc ggtggtgcct cgcgccagcg aaactggcct ggagagattt ggggggatgt
    12361 ttcatgagag ccctggccgc gcttgtcctg ctgttcccgg tgtgctcggg acaggtgatc
    12421 gcctcggcac cgaccgtcga agtgacggtt gccgttgccg ggatctgcga cgtcttccca
    12481 tacctgccgg gctgcccgcg ctaaccgcag cgaaccgccg aacacccccc gagcccgagg
    12541 aaccctcccg tgatcaccgc agagtcccag cgaaccttcg cagcgaagtt ggaagccgca
    12601 cgcgttgccc agcacttgtc gatccgcaca gtggggagga tcgccaaggc gccgcccacc
    12661 acggtgcacg gttggttgac cggcacgcac tttcctgcgt tgaccatgcg aggcaactat
    12721 ctgcgcatgg tcgagacgct tggcctgatg gcggagatgc ccgcggacat ccgcgaggag
    12781 ctctggatcg tcgacgaatc gcgagcgaag gcggcgggtt gactgctgtc cgcgtcgaac
    12841 caggcccgta ctgcgccggc agttcggccg cgggccgccg agcgtgacgg gtgatcgaga
    12901 gtcacgagtc gttggattcg gagtgtccgg tgatgcgtcc gcgtaggtcg gcagcgccga
    12961 tctcccgtgg gtgcagggtg cggtaacgct cgcagagtgc ggtgacctcg ggctcgagtt
    13021 ggctgcgcag catcatcagg ccggcgatgt aaccggacgg atcgtcttgg acatcattga
    13081 ggtagcgcag gcaggcgacg tgcatgcggt cgagctcgtc gaaggcgtcc gagtgtccgg
    13141 agaggctgtc ggacgtgaac gcgatgcggt cgcgcgtcgc cagcaccgat ttggtcgatc
    13201 gccgctcgtt ctcccgcgcg tccggggtgt cgaaggcggt cgccagctgg ccggcccgga
    13261 gcgcccggct gcggtagagc cggtcgatca gggcttgcag cagccgcagg tcgttgcggg
    13321 tctcgctgtg gcgaatcacg aagaaggacg tcagcagcgc gagtcccgcg cccacgaacg
    13381 tgccgacgac gttggacgct acgccctccc agaacgcgct catgccaaca ttgtggaaag
    13441 ccggcccgac gtagtcgaga cgtcggaccg atcaggcgct cagcactatg gaccgctcgg
    13501 taagtccgga atgaaccttc ggattgccgc tttcacgtcg tccagccagg agcggagttc
    13561 acccggcaac tcggcacccg atggggacca aaccttggcg aagcaagaag gcccgccagc
    13621 ggcggacctc ggcttgcaat tcggtggagc taacgggatt cgaa
//
LOCUS       DAOPRQ010000063        13173 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_3982_length_13173_cov_11.336484, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000063 DAOPRQ010000000
VERSION     DAOPRQ010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 13173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13173
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3982_length_13173_cov_11.336484"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(76..1263)
                     /locus_tag="PKJ61_08935"
     CDS             complement(76..1263)
                     /locus_tag="PKJ61_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013160241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HOC13987.1"
                     /translation="MPRIAPFPAIRYAPGADLQRVIAPPYDVLSPADVAELAALDEHN
                     IVHIDVPAGGDERYANAANTLTDWLASGVLVRDDEPSLTIYRMSFVDATGASRTIVGV
                     LAGLEVVDLDAGGVLPHERTTPKAVTDRLDLTRATATNLSPVWGLSLASGLTEALAEP
                     GEPVGNMVAEGVAHTVERVTDPARIAEITAILAADDVLIADGHHRYSISRTYRDEVRA
                     ASGRTDTEAEFTLTFINELVADQLSIEAIHRVYTGIALDELRAELAGSFAIEPAPTPS
                     PAMLAEMVDRGRLVLLAPDGSAEWLTPLPGAFDGVRALDGAYLEHALVGSTAEVTYQH
                     GLDEMVALVGSGEVTAGILIRPTSLAEIQRTAREGLLMPPKSTFFTPKLRTGLVIRPT
                     VSL"
     gene            complement(1355..2758)
                     /locus_tag="PKJ61_08940"
     CDS             complement(1355..2758)
                     /locus_tag="PKJ61_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003794379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HOC13988.1"
                     /translation="MSELAVHQTAIPGLLVVDLVTHGDARGWFKENWQRAKMTALGLP
                     DFRPVQHSVAYNTEAGVTRGFHAEPWDKLISIAHGRIFGAWVDLRPGVGFGRLVTLEV
                     GPETAVFVPRGVANSYQTLVPETVYSYLVNEHWSPESLASYSYVNLADETVAAPWPIP
                     LDQAVVSAADLAHPRLADARPVPPKPTVIIGAYGQLGRALQVELPDAALLDLPDFDVT
                     DPAHVAAVDWKQFGTIINAAAYTAVDAAETPDGRTACWAANVTGVAALCRVAAEHRIT
                     LVHVSSDYVFDGTAEVHTEDELISPLGVYGQTKAAGDVLVGQLPQHYLVRTSWVIGDG
                     RNFVRTMAELAERGVKPSVVADQFGRLTFASTLAAGIVHLLSSGAAYGTYNLTNDGPT
                     RSWAELAAQVFAAVGRDASDITAVTTAEYSAGKELSPRPQHSTLSLDKIKATGFEIPD
                     ADAELTAYVARLLSEIS"
     gene            complement(2755..3753)
                     /gene="rfbB"
                     /locus_tag="PKJ61_08945"
     CDS             complement(2755..3753)
                     /gene="rfbB"
                     /locus_tag="PKJ61_08945"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016666789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HOC13989.1"
                     /translation="MSTILVTGGAGFIGSNFVRHVLAHTDDSVTVLDALTYAGSRASL
                     DGLPADRFGFVHGSVCDAALLDDLVGKHDVVVHFAAESHNDNSLNDPSPFIQTNLIGT
                     YELLQAVRKHGKRYHHISTDEVYGDLELDDPAKFTETTPYNPSSPYSSTKAGSDLLVR
                     AWVRSFGVQATISNCSNNYGPYQHVEKFIPRQITNVIDGIRPKLYGAGQNVRDWIHVD
                     DHNAAVLRIIASGRIGETYLIGADGEKDNKSVIELILTLMGQPADAYDHVNDRAGHDL
                     RYAIDASRLRDELGWEPRFTSFEAGLADTIDWYRANEAWWRPLKAATEAKYAAAGQ"
     gene            3826..4707
                     /gene="rfbA"
                     /locus_tag="PKJ61_08950"
     CDS             3826..4707
                     /gene="rfbA"
                     /locus_tag="PKJ61_08950"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016669669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HOC13990.1"
                     /translation="MRGIILAGGSGTRLHPITIGTSKQLVPVYDKPMIYYPLSTLIFA
                     GVREVLVINTPHEQEAFQRLLGDGSQFGIEISYATQPEPKGLAQAFTIGADFVAGHTS
                     CLVLGDNIFYGQGLGTQLQRFSDIDGAAVFGYWVDDPTAYGVVEVGEDHRAISIEEKP
                     AEPKSNFAVPGLYFYDERVVEIAANLPPSARGEYEITDVNRVYLEAGELQVSILPRGT
                     AWLDTGKFDSLNDASNFVRTIQARQGLQVGCPEEAAWRQGFLSDDELLVRAEALAKSG
                     YGQYLKHLVEQKHRPRV"
     gene            complement(4783..6252)
                     /locus_tag="PKJ61_08955"
     CDS             complement(4783..6252)
                     /locus_tag="PKJ61_08955"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOC13991.1"
                     /translation="MTTIGALVGVQPQQREQKVATKHWLADSSVQSGLLWGTDVFVLM
                     ATAAVAMSARNLLPFKSGELLPETFSITLLVVAAWVLILTAFGAYDLRHLRAGVVEYK
                     RIVMASVVTAGGVGITCYLLSYEYSRGLVVVLFTIGSILLLLSRNLRRRMMHSLYSKG
                     MLRTPVVVAGDSQHVDAIARVLRREVWLGYDVVGALTKERIPTTIGGLPVLGGVDDVL
                     DVISEQRVDSVIFAQGSFDEPGDFRRMAWRLEDRDVQMVVVPALTDVSSQRLDVRPLA
                     GLPLVDVDHPRAIEAGRWVKRTFDIVGSALLLVLFAPVMALVALAIKLEDGGPVLFRQ
                     RRVGMKGKEFDCLKFRSMVPDAEARLAALNALNEGAGPLFKMASDPRITKVGRFIRRF
                     SLDELPQFWNALIGEMSLVGPRPALPREVEQYDSDTRRRLDVRPGLSGLWQVSGRSNL
                     SWEDTVRLDLYYVDNWSMTQDLMILARTAKAVVGSAGAY"
     gene            complement(6404..7528)
                     /locus_tag="PKJ61_08960"
     CDS             complement(6404..7528)
                     /locus_tag="PKJ61_08960"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOC13992.1"
                     /translation="MARIALIASSFLPRMGGVEVHVANIATELVRLGHTVAIWAVDQG
                     DEAPDDYRGIPVRYLPCPLPARTLPAAAGFLQQALPAWRAWHSALAADRPDVLNIQCF
                     GPNGVYASALARTTGVPLVYSHHGETSGDANDVFGSSALLRRALTRTLAGADAVTSCS
                     AHAAADLARFGRDPSTVKVISNGIDMAEPLGSPPPGLPSQYLLGIGRLVANKGFANLI
                     RAYAEARPAAGLVIGGEGPEMGRLRALAGDLDVDAHFTGRLSRPEVGALMAGADALIV
                     PSLAEAFGITVLEGWRAGVPVVVTDRGGPPEFVTDQVTGLLVDPEDVHALAEHIRRLD
                     TDPELRHRLGAAGHAAVTGFTWVGAARLYSKVFEAATSAA"
     gene            7651..8691
                     /locus_tag="PKJ61_08965"
     CDS             7651..8691
                     /locus_tag="PKJ61_08965"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HOC13993.1"
                     /translation="MLTVATSASANPMGAERYQREVLAGARAALPGTAVRHLEFRSLR
                     SPLAGDRRLPMGWLRDASAASRSILGRVVFAGAGLVHRMDLLLPPGPNEVVTLHDVVA
                     WLYPDESSPVRAAAAELKRAAAVICVSQYSAEQAVERLGVPDPVVVYNGVDERFFSAP
                     ELSEAQRRELGLVGDYVLHAGGAAKRKNLEGLAQAWPLVHRNRPQLTLALCGPEHERR
                     TRLFTGMPGVRLLGRQPEELLPSLVKSASAVVVPSWHEGFGLPALEAMAAGVPVVAAN
                     TSSLPEVVGECGILVEPQAGAIAEGLLVAAAGRAGLPELVVAGAARAREFTWQRCLAG
                     HAEVWQRLGVAG"
     gene            8861..10117
                     /locus_tag="PKJ61_08970"
     CDS             8861..10117
                     /locus_tag="PKJ61_08970"
                     /inference="COORDINATES: protein motif:HMM:NF020919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1996 domain-containing protein"
                     /protein_id="HOC13994.1"
                     /translation="MRAVVRSVLAFALVAGGLSLAPTPALAVPEPSFRVVCALTRVAP
                     DDPIVFPGQPGRSHLHNFFASQVTAATTVDSLLAQSSTNCSRGYGGVDFSGYWVPALL
                     KNGSPVNSESVRVELAAYYKRAGGASGVAVNQAIPRGLRMIAGDMMAGGPQEMVYYKC
                     AKNEDAGSQSKLGRGFPSCASDEMLIAELKFPDCWDGVNLDSANHKSHVAYSTGSDAR
                     CSASHPVKLPQLTFELWYHGVNGPASSLSWASGDQYSFHGDVISAWNTTAQANLVNQC
                     INTARDCNPLVFGSVPVGAVSQAQLDAQLVGTMPGGNPSTGAPSTGAPSTTASSFTKI
                     ATPTVTGKARVGRKLTAKPGTWSPVPQALSYRWYRNGKAIKGATQVSYKLKKADRGKR
                     IKVKVTATKSGYVTVVKASKATAKVR"
     gene            complement(10178..11170)
                     /locus_tag="PKJ61_08975"
     CDS             complement(10178..11170)
                     /locus_tag="PKJ61_08975"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOC13995.1"
                     /translation="MAETSNQTAAGLTVAVVIPTFNRPDRVRDCLEHLATQEVRPNRV
                     VVVDSSADARTETIVRGFPGVLYVRNPMGRGRTPESRQIGYSLTTEDVIAFLDDDANA
                     RPTWLRELVARYDQPEIGGVGGSALNGIEGERSDGADQIGRLLPDGRITGYFAADPGR
                     DLDVDHLLGANMSFRRSAIEAVGGIHGDYPGTCMREESDLALRVREAGFRLVYTPAAV
                     VDHLPGEYAKGRRFDRRYVYYANRNTIVLFGRVYGLDHPIVRRYIGTAMREVGFEIAR
                     GVRGLGSIGRSGPQRAARTFVGGLTRAGAVAGGVAAGLPAAAVAIRRDRERFNS"
     gene            11385..12512
                     /locus_tag="PKJ61_08980"
     CDS             11385..12512
                     /locus_tag="PKJ61_08980"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOC13996.1"
                     /translation="MSSRVPVALAHDYVTQRGGAERVAAIMAEAFTGAPLYTTLYDPE
                     GTFADFEGLDIRTSVLNRLPWLRRHHRLALPLLAPAVSTMRVDADVLLASSSGWAHGI
                     RCTGRTVVYCHAPARWLYQKDVYLGADEGPPSQRAQRFLAAATLGMVGPALRRWDAGK
                     AKTADRYLVNSTVIRTAVAEVYGIEAEVLAPPPAMTPGGPMRRPDGVTEPFMLCVARL
                     LPYKNVDVVIRALDRLDGLGLVVVGRGPDEQRLRALAAHNPSLRILGGASEAELRWLY
                     ANAVGLVAASYEDFGLTPLEAAGFGKPTAALRAGGYLDTIDPVVNGVFFENPDPASVA
                     DGVAALVERTWDAAAIGGHADRFGRARFIERLRGVVAEFGA"
     gene            12643..>13173
                     /locus_tag="PKJ61_08985"
     CDS             12643..>13173
                     /locus_tag="PKJ61_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC13997.1"
                     /translation="MSLDARLVIFGAVRAWAPVVLAMLVLVVGAARLWQRRYLATLTG
                     AVTAAGVLLAAELLKRSLPRPRLGVASYDHNTLPSTNVAACAVLLLVLVRLSPAAGRQ
                     LSVGLAIPALAVIGYASVTTSAHRPSDVLSAIWLAAAGFAMTRPGGGSTVGAAIAFAG
                     SLGAVGMGGLWPAGGLA"
BASE COUNT         2101 a         4300 c         4594 g         2178 t
ORIGIN      
        1 cgtttgccac cgatacttcg gcagcaaacg tccacttcgg tagcaaacgc cggtttggga
       61 gcgggccagg cgcggctaga gggacaccgt cgggcggatc accaggccgg tgcggagctt
      121 cggggtgaag aacgtcgact tgggcggcat cagcaggccc tcgcgggcgg tgcgctgaat
      181 ctcggccaac gaggtgggcc gaatcaggat gcctgcggtc acctcgccgg agccgaccag
      241 tgccaccatc tcgtccaggc cgtgctgata ggtcacctca gctgtgctgc cgacgagggc
      301 gtgctccagg tacgcaccgt ccagagcgcg gacgccgtcg aaagcgccgg gcaatggggt
      361 cagccactcc gccgagccgt ccggggcgag cagcaccagg cgaccgcggt cgaccatctc
      421 ggccagcatc gccggggacg gggtcggtgc cggctcgatc gcgaacgaac ctgcgagctc
      481 ggcgcgcaat tcgtcgagcg cgatgccggt gtagacccgg tgaatcgcct cgatgctgag
      541 ctggtcggcg accagctcgt tgatgaaggt gagcgtgaac tcggcctcgg tgtcggtacg
      601 tccggacgcc gcgcgcacct cgtcgcggta ggtgcgtgag atcgagtagc ggtggtgacc
      661 gtcggcgatc aggacgtcgt ccgcggcaag aatcgcggtg atctcggcga tccgggccgg
      721 atcggtgacg cgctcgacgg tgtgggccac gccttcggcg accatgttgc cgaccggttc
      781 gccgggctcg gccaacgcct cggtgaggcc actagccagg gagagacccc acaccgggga
      841 gaggttggtc gcggtcgcgc gggtgaggtc gagccggtcg gtgaccgcct tcggtgtggt
      901 gcgttcgtgc ggcagtacgc cgccggcgtc cagatcgact acttcgaggc cggccagcac
      961 gccgacgatg gtgcggctgg cgccggtcgc gtccacgaac gacattcggt agatggtcag
     1021 gctgggctcg tcgtcgcgca ccagaacacc ggacgcgagc cagtcggtca gcgtgttcgc
     1081 ggcattggcg tagcgctcgt cgccgccggc gggcacgtcg atgtggacga tgttgtgctc
     1141 gtcgagcgcc gccagttcgg ccacgtccgc aggtgaaagg acatcgtaag gcggcgcgat
     1201 cactcgctgc aggtcagcgc ccggggcata gcggatggcc ggaaacggtg cgattcgagg
     1261 catgagcaaa cgctaccggc tctcgctggg ccagctgaac cgtctccaat agcgctccct
     1321 gaggagtcgt gatgagggac gagggaccct cggctcagga gatctcgctg agcagtcggg
     1381 ccacgtaggc ggtcaactcg gcgtccgcat cggggatttc aaagccggtg gctttgatct
     1441 tgtcgaggct cagagtcgag tgctgcggac ggggtgatag ctccttgccg gcagagtatt
     1501 ccgccgtcgt taccgcggtg atgtcggacg cgtcccggcc cactgcggcg aagacctgtg
     1561 cggccagctc ggcccaggat cgggtggggc cgtcgttggt caggttgtac gtgccgtacg
     1621 cggccccgct gctcagcagg tgcacgatgc cggcggccag cgtcgaggcg aaggtcagcc
     1681 ggccgaactg gtcggcgacc acgctcggct tcacgccgcg ctcggccagc tcggccatcg
     1741 tccggacgaa gttgcggcca tcgccgatca cccagctggt gcgcaccagg tagtgctgcg
     1801 gcaactgtcc gaccagcacg tcgccggcgg ccttcgtctg gccatacacg cccagcggcg
     1861 agatcagctc gtcctcggtg tgcacctcgg cggtgccgtc gaacacgtag tcactggaga
     1921 catgcaccag cgtgatccgg tgctcggcgg ccacccggca gagcgccgcc acgccggtga
     1981 cgtttgccgc ccaacaggcc gtccgaccgt ccggtgtctc ggccgcgtcc accgcggtgt
     2041 aggcggcggc gttgatgatc gtcccgaact gcttccagtc gaccgcggcc acgtgcgccg
     2101 gatcggtcac gtcgaagtcg ggcaggtcga gcagtgccgc gtccggcagc tctacctgca
     2161 gcgcgcgacc cagttggccg taggccccga tgatcaccgt cggtttcggt ggcaccggac
     2221 gggcgtccgc cagccgcggg tgcgccaggt cggcggccga gaccacagcc tgatcgagcg
     2281 ggatcggcca aggcgcggcc acggtctcgt cggcgaggtt gacatagctg taggacgcca
     2341 gcgactccgg gctccagtgc tcgttgacca gatacgaata gacggtctcc ggcaccagcg
     2401 tctggtagct gttcgccacc ccgcggggca cgaagaccgc cgtctcgggg ccgacttcca
     2461 gggtgaccag ccggccgaaa ccaacgcccg gacgcaggtc tacccaggcg ccgaagatgc
     2521 gtccgtgggc gatggagatc agcttgtccc acggctcggc gtggaagccg cgggttacgc
     2581 cggcctcggt gttgtaggcc acgctgtgct gcaccggacg gaagtcgggc aggcccagcg
     2641 cggtcatctt ggcccgctgc cagttctcct tgaaccagcc acgcgcgtcc ccgtgcgtga
     2701 ccaggtcgac gaccagcagg ccggggatcg cggtctggtg gacggcgagc tcgctcactg
     2761 gccggccgcc gcgtacttgg cttctgtcgc agctttgagc ggacgccacc aggcctcgtt
     2821 ggctcgatac cagtcgatgg tgtcggccag acccgcctcg aacgaggtga accgcggttc
     2881 ccagccgagc tcgtcccgca gccgggacgc gtcgatggcg tagcgcaggt cgtggcccgc
     2941 gcggtcgttg acgtggtcgt aggcgtccgc cggctggccc atcagggtga ggatcagctc
     3001 gatcacgctc ttgttgtcct tttcgccgtc cgcgccgatc agataggtct cgccgatgcg
     3061 tccggacgca atgattcgca gtacggcggc gttgtggtcg tcgacgtgga tccagtcgcg
     3121 gacgttctgg ccggcgccgt acagcttggg gcggatgccg tcgatgacgt tggtgatctg
     3181 gcgggggatg aacttctcga cgtgctggta gggcccgtag ttgttcgagc agttcgagat
     3241 cgtggcctgg acgccgaacg agcgcaccca ggcgcgcacc agcaggtcgg aaccggcctt
     3301 ggtcgacgag tacgggctgg acgggttgta cggcgtggtc tcggtgaact tcgccgggtc
     3361 gtcgagttcg aggtcaccgt agacctcgtc ggtcgagatg tggtggtagc gcttgccatg
     3421 cttgcggacg gcctgcagca gctcgtaggt gccgatcagg ttcgtctgaa tgaacggcga
     3481 cgggtcgttc agcgagttgt cgttgtgcga ctcggcagcg aagtgcacga ccacgtcgtg
     3541 cttgccgacc aggtcgtcga gcagggccgc gtcgcagacc gagccatgga caaaaccgaa
     3601 ccggtcagcg ggcaacccgt ccagcgaggc tcgcgagccg gcgtaggtca gggcgtccag
     3661 cacggtaacc gagtcgtcgg tgtgggccag gacgtggcgg acgaagttgc tgccgatgaa
     3721 gccggcgcca ccggttacca ggatcgttga catggtcttg atcctagagc gctgggaacg
     3781 ggttcgcgct gcggtcgagt cctccgacgt ggcaggatgc caggcatgcg cggcatcatt
     3841 ctggctggcg gctccggcac ccggctgcac cccatcacga tcggcaccag caagcagctc
     3901 gttccggtgt acgacaagcc gatgatctac tacccactgt cgacgctgat cttcgctggc
     3961 gtccgcgagg tgctggtgat caacaccccg cacgagcagg aggcgttcca gcggctgctc
     4021 ggggacggtt cccagttcgg catcgagatc agctacgcca cccagcccga gccgaagggg
     4081 ctggcgcagg cgttcactat cggcgcggat ttcgtggccg ggcacacgtc ctgtctggtg
     4141 ctgggcgaca atatcttcta cggccagggc ttggggacgc agctgcagcg gttcagcgac
     4201 atcgatggcg ccgcggtctt cggctactgg gtggacgacc cgaccgcgta cggcgtggtc
     4261 gaggttggcg aagaccaccg ggccatctcg atcgaggaga agccggccga gccgaagtcg
     4321 aacttcgcgg tgccggggct gtacttctat gacgagcgcg tggtcgagat cgcggcgaac
     4381 ctgccgccgt ctgcgcgcgg tgagtacgag atcacggacg tgaaccgggt ctacctcgaa
     4441 gccggtgaac tgcaggtttc gatcctgccg cgggggaccg cctggctcga caccggcaag
     4501 ttcgactcgc tgaacgacgc tagcaatttc gtccggacga ttcaggcccg gcaggggctg
     4561 caggtgggtt gcccggagga agccgcgtgg cggcagggat tcctgtccga cgatgaacta
     4621 ctcgttcggg ccgaggccct ggccaagtca ggctatggcc agtacctgaa gcatctggtg
     4681 gagcagaagc accgcccccg ggtgtagtcg ggtcgcaccc gggagcggcg ccggagggga
     4741 agggctcccc acgaggtcac cagccggccc cagttattcc ggctagtagg ctcccgcgga
     4801 gccgacgact gctttcgcgg tccgggccag gatcatcaga tcctgggtca tggaccagtt
     4861 gtcgacgtag tagaggtcga ggcgaaccgt gtcctcccag gacaggttcg agcgtccgga
     4921 gacctgccac aggccggaca ggccgggacg gacgtccaac cggcgacgcg tgtcagagtc
     4981 gtactgctcg acctcgcgcg gcaatgccgg acgcggcccg accaggctca tctcgccgat
     5041 cagggcgttc cagaactgcg gcagctcgtc cagcgagaac cggcggatga agcggccgac
     5101 cttggtgatc cgcggatcgg aggccatctt gaacagcggg ccggcaccct cgttgagggc
     5161 attcagcgcg gccagcctgg cctcggcgtc gggcaccatg ctgcggaact tgaggcagtc
     5221 gaactccttg cccttcatgc cgactcgacg ctggcggaac agcaccgggc caccatcctc
     5281 gagcttgatc gccagggcga ccagggccat caccggcgcg aacagcacca gcagaagggc
     5341 tgagccgacg atgtcgaagg ttcgcttgac ccagcgtccg gcctcgatcg cgcgcggatg
     5401 gtccacgtcc accagcggca gcccggccag cggacgcacg tccaggcgct gcgaggagac
     5461 atcggtgagc gcaggcacca cgaccatctg tacgtcccgg tcctccagcc gccaggccat
     5521 ccggcggaag tcgcccggct cgtcgaacga gccctgcgcg aagatgaccg aatccacccg
     5581 ctgctcggag atgacatcaa gcacgtcgtc gacgccgccc agcaccggca gcccgccgat
     5641 agtagtcggg atgcgctcct tggtcagggc gccgaccacg tcgtagccga gccacacctc
     5701 tcgacgcaat acgcgagcga tcgcgtccac gtgctggctg tctccggcca cgaccaccgg
     5761 cgtgcgcagc attcccttgc tgtagagcga gtgcatcatg cggcgacgga ggttgcgcga
     5821 cagcagcaac aggatcgatc cgatcgtgaa caacaccacg accagtccac gggagtactc
     5881 gtagctgagt aggtagcagg tgatgcccac cccgccggca gtgacgaccg aagccatcac
     5941 gatgcgcttg tactccacca cgcccgcccg caggtgtcgc aggtcgtacg cgccgaaagc
     6001 cgtgaggatc agtacccagg ccgccacgac cagcaaagtg atgctgaaag tctcgggcag
     6061 taactcaccg ctcttgaagg gcagcaggtt cctcgccgac atggcgaccg cggcggttgc
     6121 catgagcacg aaaacgtcgg tgccccaaag cagtcccgat tggacggacg aatcagccag
     6181 ccaatgtttg gtggctactt tttgctctct ttgctgtggc tgtacgccaa ccagcgcccc
     6241 gatagtggtc attgcccccc gactcagacc agtgaacccc agcccttccc cccgaacgac
     6301 tggacgagtc ttcgcgcccc cgcactaaca actcgcaggt tgagcatatc gccgcgggga
     6361 tccgctacaa cgagagtttc gcgagatctc gtacggaaac ttgctaagca gcggaggtgg
     6421 cggcttcgaa taccttcgag taaaggcggg cagcgccaac ccaggtgaac ccggtcacgg
     6481 ctgcgtgccc ggccgcgccg aggcggtggc gcagttcagg gtcggtgtcg agccggcgga
     6541 tatgctcggc gagtgcatgg acgtcctcag ggtcgaccag cagcccagtc acctggtcgg
     6601 tgacgaactc cggcgggccg ccccgatcgg tgaccaccac gggcaccccg gcgcgccaac
     6661 cttcgaggac ggtgatgccg aacgcctcgg cgaggctggg cacgatcagc gcgtctgctc
     6721 ccgccatcaa agctcccacc tctgggcggc tcaatcgtcc ggtgaagtgc gcgtcgacat
     6781 cgaggtcgcc ggccagtgcc cgcaggcgac ccatctccgg gccctccccg ccgatcacta
     6841 ggcccgcagc cggtcgagcc tcggcgtagg cacggatcag gttggcgaag cctttattgg
     6901 cgaccagccg gccgatgccg agaagatatt gcgatggcag ccccggcggg ggcgacccca
     6961 gcggctcggc catgtcgatg ccgttcgaga tcactttgac cgtgctggga tcgcggccga
     7021 agcgggcgag gtcggcagcc gcgtgggccg aacacgacgt caccgcgtcg gctccggcga
     7081 gcgtgcgggt cagcgcccgg cgcagcaggg ccgaggatcc gaagacgtcg ttggcgtcgc
     7141 cggacgtctc cccgtgatgg ctgtaaacaa ggggcacgcc ggtcgtccgc gccagtgcgg
     7201 acgcgtacac accgttcggc ccgaagcact ggatgttcag cacgtccgga cggtcggcgg
     7261 cgagcgcgct gtgccacgcc cgccacgcag gaagggcctg ttgcaggaac ccggccgccg
     7321 cgggcagagt acgcgctggc agcggacagg gcaggtagcg caccggaatg ccccggtagt
     7381 cgtcgggtgc ctcgtcgccc tgatctacgg cccagatcgc cactgtgtgg ccgaggcgga
     7441 cgagctcggt ggcgatgttg gccacgtgca cctcgacacc acccatccga ggcagaaatg
     7501 agctggcgat gagtgcgatc ctggccatgg ttgtccccct cgcgctgggc tggtctgcca
     7561 tcatcgttcc actaggcact gatgtcttcg cagtgaacgg cgccgcaacc cggaaaaggc
     7621 ggccctgctc actactcgac aaggatggtc atgcttaccg ttgccacttc cgcttcggcc
     7681 aatccaatgg gcgctgagcg ataccagcgt gaggtgctgg cgggtgctcg tgccgcgctg
     7741 cccggaacgg cggtccgtca cctcgagttc cgctcgctgc gttcgccgct ggcgggagac
     7801 cgccgcctac cgatgggttg gctacgcgac gcgtcggccg cgtcccggtc gatcctgggc
     7861 cgagtggtct tcgccggcgc cggactggtg catcgcatgg atctgctgtt gccacccggc
     7921 cccaatgaag tggtcaccct gcacgatgtg gtggcttggc tctacccgga cgagtcgtcg
     7981 ccggtgcggg cagcggcagc cgagctcaaa cgcgcagccg cggtgatctg cgtctcgcag
     8041 tactccgcgg agcaggcagt cgagcgtctt ggcgtgcccg acccggttgt ggtctacaac
     8101 ggagtggacg aacgcttctt ttccgcaccg gaactcagcg aggcgcagcg tcgtgagctg
     8161 ggcctggtag gggactacgt gctgcacgcc gggggcgcgg ctaagcgcaa gaacctggag
     8221 ggactcgcgc aggcctggcc gctggtgcac cggaaccgtc cccagctcac ccttgccctc
     8281 tgcgggccgg agcacgagcg acgcacccgg cttttcacag gcatgcccgg ggtgcgactg
     8341 ctgggaaggc agcccgagga gttgcttccc agcctggtga agagcgcgag cgcggtggtg
     8401 gtgccgtcct ggcatgaggg cttcgggctg cccgcgctgg aagccatggc cgcgggggtg
     8461 ccggtcgtgg cggcgaacac gtcctcgttg cccgaggttg taggcgaatg cggcatcctg
     8521 gtcgagccgc aagcgggcgc cattgccgag ggcctcctgg tggctgccgc cggccgggcc
     8581 ggattgcccg aactggtggt ggccggtgcg gccagggcgc gcgagttcac ctggcagcgt
     8641 tgcttggccg gtcacgcgga ggtgtggcag cggctcgggg tcgccggctg agtcgaggcg
     8701 gcgggctctt cggacgattg cgggatatcc accaaccttg accttggcca agaattagct
     8761 tagaatttct cagtaatccc tttccctggt gacgcactct ggagattggt gccctccgac
     8821 tcgtgcggtg atgcgtcgct ctgagtgttc cggaggctcc atgcgagcag tagttcggtc
     8881 tgtgctggcc ttcgctctgg tggctggtgg tctgtcgtta gcgcctacgc cggcgctcgc
     8941 agtaccggag ccgtccttcc gggtggtctg tgcgctcacc cgggtggcgc cggacgatcc
     9001 gatcgtgttt cccgggcagc ccggcagatc gcacttgcac aacttcttcg cttcgcaggt
     9061 cacggccgcc acgacggttg attcgctgct cgcgcagtcg tcaaccaact gctcgcgcgg
     9121 ctacggcggc gtcgatttct ccggctattg ggttccggca ctgctcaaga atggctcccc
     9181 ggtgaactcc gagtccgttc gggtcgaatt agcggcctac tacaagcgcg ccgggggagc
     9241 gtcgggagtc gcggtcaacc aagcgattcc tcggggcctg cgaatgatcg ccggcgacat
     9301 gatggctggc ggaccccagg aaatggtcta ttacaagtgc gccaagaatg aagatgccgg
     9361 cagccagagc aagctcggcc gtggcttccc cagttgtgct tccgatgaga tgctgatcgc
     9421 ggagctgaag ttccccgatt gctgggacgg ggtgaacctg gactcggcca accacaaatc
     9481 gcatgttgcc tactccaccg gcagcgatgc ccgttgctcg gccagccacc cggtcaagtt
     9541 gccgcagctc actttcgaac tctggtatca cggggtcaac ggcccagcct caagcctctc
     9601 gtgggccagc ggtgaccagt acagtttcca cggcgacgtg atctcggctt ggaacaccac
     9661 agcccaagcg aacctggtta accagtgcat caacaccgcc cgcgactgca acccgctcgt
     9721 attcgggagc gtcccggtcg gtgccgtttc ccaggcacag ctcgacgccc agttggtcgg
     9781 gacgatgccc ggcggcaacc catccaccgg tgccccctcg accggtgccc cgtccaccac
     9841 cgcgagcagt ttcaccaaga tcgccacccc gacggtcacc ggcaaggcca gggtcggacg
     9901 gaaactgact gccaagcccg gcacgtggag ccccgtcccc caagcgctga gctaccgctg
     9961 gtaccgcaac ggcaaggcga tcaagggggc cacccaggtc agctacaagc tgaagaaggc
    10021 cgatcgcggc aagcggatca aggtgaaggt gaccgcgacg aagtcgggtt acgtcaccgt
    10081 cgtgaaggcg tcgaaggcga ctgcaaaggt ccgctgacga aggccgcgcg gaggctggcg
    10141 acgcgatcgg gccagaaaaa gacctggccg gctggggtca gctgttgaag cgctcccgat
    10201 ctcggcggat cgcgaccgca gccgcaggaa ggcccgccgc cacacccccc gcgaccgcgc
    10261 ctgcgcgagt gagcccgccg acgaaggtgc gggcggcgcg ttgcggaccg gagcgtccga
    10321 tagaaccaag gccgcgcacg ccgcgggcta tctcgaaacc gacctcacgc atggccgtgc
    10381 cgatgtagcg gcggacgatc ggatgatcca gtccgtacac ccggccgaag agcacgatcg
    10441 tgttccggtt cgcgtagtag acgtagcggc gatcgaatcg cctgcccttc gcgtattcgc
    10501 cgggaaggtg gtcaaccaca gcggctgggg tgtagaccag ccgaaagccc gcttcccgca
    10561 cccgtagggc gagatcgctc tcctcccgca tgcaggttcc ggggtagtcg ccgtgaatgc
    10621 cgccgaccgc ttcgatggcc gaccggcgga aggacatgtt cgccccgagc agatggtcga
    10681 cgtcgaggtc ccgtccggga tcggcggcga aatagccggt gattcggccg tccggcagca
    10741 gccgtccgat ctggtcggcg ccgtcgctgc gctcgccctc aatcccgttg agcgcgctcc
    10801 cgccgacgcc gccgatctcg ggctggtcat agcgagcgac cagttcccgc agccaggtag
    10861 gcctggcgtt ggcgtcgtca tcgagaaagg cgatcacgtc ctcggtagtc aacgagtagc
    10921 cgatctggcg cgactccggg gtgcgtcccc gtcccatcgg gtttctgacg tagagcaccc
    10981 ccgggaagcc gcgaacaatc gtctcggtgc gggcgtccgc tgagctgtcc accactacga
    11041 cacggttcgg acggacctcc tgtgtcgcaa ggtgttccag acagtcgcgg acccggtcgg
    11101 gtcgattgaa cgtcgggatc accacggcga cggtcaggcc cgcagcggtc tggttgctgg
    11161 tttcggccat ggcgactcct cgagatcaac tccgaccccg tcagcccccg gtcggccagg
    11221 tgcaggccac tgtagtgcgg ctgggcacgt tcgcgaagcc tcgccagtgt ggacaccgtg
    11281 agtagggtaa ggccaagccg gcccccaccg cggagcaggt tcccggggga tgtggaggcg
    11341 gtcgcatggc ggcaggggac gtcgttcgac gggcgggacg cgcggtgagc agtcgggtcc
    11401 cggttgccct ggcgcacgac tacgtgaccc agcgcggcgg tgcggaacgg gtggcggcga
    11461 tcatggccga agcattcacc ggggctccgc tgtacacgac gctctatgac cctgaaggga
    11521 ctttcgccga tttcgagggc ttggacatcc gcacctcggt gctgaaccgg ctgccctggc
    11581 tgcgtcgcca ccaccgattg gcgctgccgc tgctcgcccc ggcggtgtcg acgatgcggg
    11641 tggacgcgga cgtcctgctg gcgagctctt caggctgggc acacggaatc cggtgcaccg
    11701 gtcgcacggt ggtctactgt cacgcgccgg cccgctggct ctaccagaag gacgtgtacc
    11761 tgggcgcgga cgaggggccg cccagccagc gtgcccagcg cttccttgcc gcggccacgc
    11821 tgggcatggt cgggccggcg ctgcgccgct gggatgcggg caaggcgaag acagcggacc
    11881 gttacctggt gaactccacg gtgatccgga ctgcagtcgc cgaggtctat ggcatcgagg
    11941 ccgaagtgct cgcacctccg ccagcgatga ctccgggcgg cccgatgcgg cgcccggacg
    12001 gagtgaccga gcccttcatg ttgtgtgtgg cccggttgct cccgtacaag aacgtggacg
    12061 tcgtgatccg ggcactcgac cggctggacg ggcttggcct cgtcgtggtg ggtcgaggtc
    12121 cggacgaaca gcggttgcgt gccctggcgg cgcacaaccc gtcgcttcga atactcggcg
    12181 gcgccagcga agcagagttg cgatggctct acgcgaacgc cgtaggcctg gtcgcggcct
    12241 cgtatgagga tttcggactg accccgctgg aggcggccgg tttcgggaag ccgactgccg
    12301 cgttgcgagc cggcggttac ctggacacga tcgacccagt ggtcaacggc gtcttcttcg
    12361 agaaccctga cccggcttcg gtggccgacg gcgtggccgc tctggtcgag cgcacctggg
    12421 acgcagctgc gatcgggggc catgccgacc gtttcggcag ggctcggttc atagagaggc
    12481 tccgtggtgt cgtcgcagag ttcggcgcct gagccgttgc gggggcggtt gctggcgtcg
    12541 tcgggctacc ttgccgccgc cgccgcgacc tacgcaatgc tggtgtggac gccttggggg
    12601 caatcattgg atgcggccag cctgggcgct ggattcggtt ggatgagtct cgacgcacga
    12661 ctcgtcattt tcggcgcggt gcgggcgtgg gcgcccgtgg tgctcgcgat gctggttctt
    12721 gtggtgggtg cggcacggct gtggcaacgc cgatatctgg ccaccctgac cggcgcggtg
    12781 acggccgccg gcgtgttgtt ggccgcagaa ctattgaagc gctcgctgcc gcggccgcgc
    12841 cttggcgtcg catcctatga tcacaacacc ctgccgagta cgaacgtcgc agcctgcgcg
    12901 gtgctgctgc tggtcctcgt ccggctatcc cctgcggcag gtcggcagtt gtcagtcgga
    12961 ctcgccattc cggccctggc cgtgatcggc tacgcctcgg tgacgacctc ggcccaccgg
    13021 ccaagcgacg tgttgagcgc gatctggctg gccgcggccg gcttcgcaat gacccgtccg
    13081 ggcggcggtt ccacggtcgg ggcggcgatt gcgttcgccg ggtcgctcgg cgccgtcggc
    13141 atgggcgggc tctggccggc cggaggatta gcg
//
LOCUS       DAOPRQ010000064        13105 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_4002_length_13105_cov_10.448352, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000064 DAOPRQ010000000
VERSION     DAOPRQ010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 13105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13105
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4002_length_13105_cov_10.448352"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..972
                     /locus_tag="PKJ61_08990"
     CDS             <1..972
                     /locus_tag="PKJ61_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002518559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HOC13998.1"
                     /translation="GLTAIISVKLAEPQFEGQTKTKLGNTEARSFVQKVVNDLLGDWF
                     EKNPADGKDIVRKSQAAASARVAARKARDLARSRKGLLNKGQYGKLSDCSSTEPKECE
                     VFIVEGDSAGGSAKGGRDPRTQAILPIRGKILNVEKARLDKILQNKEVEAIFNTLGTG
                     VQDDFDVDKLRYHKIVLMADADVDGAHIRTLLLTLLFRFMKPLINAGHIYLAQPPLFR
                     LRWSNAPHELAYSDEERDRLRDTGIEAGKKLPSVNPIQRYKGLGEMNAEDLWDTTMDP
                     DKRSLLQVTLEDAAAADQMFSILMGEDVEQRRSFIQRNAKDVRFLDI"
     gene            1068..3719
                     /gene="gyrA"
                     /locus_tag="PKJ61_08995"
     CDS             1068..3719
                     /gene="gyrA"
                     /locus_tag="PKJ61_08995"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020386711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOC13999.1"
                     /translation="MTDGPDEPTDVELETVPENDALAPTEAKTEEIDLQVEIQRSYLD
                     YAMSVIVGRALPDVRDGLKPVHRRVLYAMYDGGYRPDRGWNKCSRVVGEVMGLYHPHG
                     DSAIYDTLVRLAQPWVMRAPLVSGQGNFGSPGNDPAAAMRYTECKMAPLSMEMVRDIT
                     EDTVDFKPNYDNREMEPTVLPARFPNLLVNGSTGIAVGMATNIPTHNLREVASAVQWS
                     LEHPEASKEELLEAAMERVKGPDFPNGALIVGRKGIEDAYRTGRGSVIMRAVIDIEED
                     KNGRTQLVVTELPHMCNPDNLAVKIAELVNAGKLSGIADIRDDSSARTGQRLVILLKR
                     DAQPRVVMNNLYKHTQLQDTFGCNMLALVDEVPRTLRLDQFITYWVRHQIQVIQRRTA
                     FRLKEAEAKAHLDRGLVKALNRLDEVLALIRASRTADAAREGLKDLLDIDDAQALFIL
                     DQQLRRLASMEIQQINERLAAREIAIADLKDILAKPERQRQIVSTELAEIVDKYGDER
                     RTRIIAADGDLSDEDFIPDDDMVVTITRGGYAKRTRTDLYRTQKRGGKGVRGATLRAD
                     DEVDHLFATTNHHWILFFTNLGRVYRAKVWQLPEAGRDAKGGHVAGLLSFLPDERIAE
                     VLTLRAYDDAPYLLLATKNGLVKKTELRAYDSPRQAGVIAINFRDADDELIGASLCSP
                     VDDVLLVSKKGQAIRFAADDEQLRPMGRATSGVTGMKFRPGDALLSMSIIGADMPEDD
                     RYVFTVTDGGFAKRTAVSEYRQQGRGGLGIKAMKLNEDRGSLVGGLVVQESDEVIAIK
                     VSGQITRSAVAEVAAKGRDTMGVKFVGVADDDAVSVIAINPETAADDDEHAVVAEDAA
                     LHGTVELQEPEVATTADQTTPEEEPGD"
     gene            3712..4437
                     /locus_tag="PKJ61_09000"
     CDS             3712..4437
                     /locus_tag="PKJ61_09000"
                     /inference="COORDINATES: protein motif:HMM:NF023511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3566 domain-containing protein"
                     /protein_id="HOC14000.1"
                     /translation="MTDRKGTADPYAGPAATNGSEWSVEGWTVSEDGLSDTVVRMPKI
                     TDVDSQPTFVAPADRPDEPVDPLGDGAPALPSMAVTAPVPPVPAAVAVAPVPPVPAAA
                     AAVPVSAPKPKKTGLRRTRKARLRLSRLDPWSVMKTSFMFSIAFGIMFWVATWLLWTV
                     IVQSGLFEAINAQILNIIQNPSNTTTFRIEDYVNTNKVLGIAALLAVVNVVISTALGT
                     LFAFLYNLSANVLGGLELTLAED"
     gene            4685..4758
                     /locus_tag="PKJ61_09005"
     tRNA            4685..4758
                     /locus_tag="PKJ61_09005"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4719..4721,aa:Ile,seq:gat)
     gene            5119..5619
                     /locus_tag="PKJ61_09010"
     CDS             5119..5619
                     /locus_tag="PKJ61_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016668922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOC14001.1"
                     /translation="MSTQTTDATTVLPPADMEAMLDLSRFLAHVTEPAALLGPDGQTV
                     PLPIEAYQVLVKVVESMRAGKAITVAPLDQRLTTQEAADFLGISRPTLVKLLEQGEIS
                     YERPAAGRHRRVRLSDVLAYQVRKRQERRVTLDQMTADAVEAGLYDVVPDYSEALRAA
                     RKRRAS"
     gene            5623..6195
                     /locus_tag="PKJ61_09015"
     CDS             5623..6195
                     /locus_tag="PKJ61_09015"
                     /inference="COORDINATES: protein motif:HMM:NF024861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HOC14002.1"
                     /translation="MARYSAFLDACVLVPIALADTLLRLAEAGLYRPLWSDRVLDEVI
                     DAIGGIHPELASGPARSRTDAMQSSFEGACVTDWEELVAGIALPDPDDRHVVAAALQG
                     RADMIVTANVRDFPSEILEPMGLEVQHPDEFLLNQLDLEPDITIATLHRQADATRRPA
                     ITTKVLLEHLSRCGVPGFAATAAHQLWRAT"
     gene            6415..6750
                     /locus_tag="PKJ61_09020"
     CDS             6415..6750
                     /locus_tag="PKJ61_09020"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOC14003.1"
                     /translation="MSFAYLPETRTRDQVDALPGITVLQFGTDWCGYCRAAEPAIASV
                     LSEADGVRHVLVEDGKGRPLGRSFRVKLWPTLVFLADGAEIARVVRPWGTGELVEALA
                     RVRAAAEAA"
     gene            7157..7717
                     /locus_tag="PKJ61_09025"
     CDS             7157..7717
                     /locus_tag="PKJ61_09025"
                     /inference="COORDINATES: protein motif:HMM:NF014427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOC14004.1"
                     /translation="MPIAGLAPGGPVVTTVKFNQNQLRLVKRKTFQLEEGVYYTNVHP
                     AYAAAVTWGSSNSKIATVSGDGIVKAKKSGTVTITATTNELTEAGAKASTSIRITVVA
                     KKPKVKVTKVSASVPTSLKLGQSAYITGSYASAKAAGVKVSYASSNDSVASVDAVGRI
                     VAKNAGTLRITVKAGSKTRTYPVSVG"
     gene            complement(8291..8974)
                     /locus_tag="PKJ61_09030"
     CDS             complement(8291..8974)
                     /locus_tag="PKJ61_09030"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOC14005.1"
                     /translation="MNGARGRYSGSDEKIASIVDTAFRVFLEQGYHNSSLRQIAAACG
                     LTDGGLLHYFPSKSHLLSAVARRHADEKRASWSQLEAPLTFFEELGLAWRSKALSQEN
                     PRWIEFSVAATSEGSNPFSRTHADHAEMYRSIVSRLTGRYQICKDNGELRPEVDPAAL
                     ARARMALATGYSTQWVLEDQDFDMGSEYLESLAWLAASVSTERLTIEEARSRIVTEAG
                     KFTAVTPSG"
     gene            complement(9014..9538)
                     /locus_tag="PKJ61_09035"
     CDS             complement(9014..9538)
                     /locus_tag="PKJ61_09035"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOC14006.1"
                     /translation="MLEFALFALPSLIYFLVRGRTRDAAQVLGLRWPNLSGWLYGAAL
                     ALISIAIAWTATIGVPAAVLHGSGTSGRIVNAWGALAVVLAAAGEELFFRGFLQGIIA
                     RHWGASVGIIVQGVAFLLPHLLLLTVSPSLIWLVAGQFVIGLMLGWLRQHTASVAPGV
                     LAHVIANLLAGLLS"
     gene            9830..10132
                     /locus_tag="PKJ61_09040"
     CDS             9830..10132
                     /locus_tag="PKJ61_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14007.1"
                     /translation="MLFDNDRVRVLEYRDGPGDATRPHTHPDSVMITLSAFRRRLRSG
                     GRETDIELPAFQARWLDGQEHSGTNIGQTPTHTIFVELKEPAERGDAPGRLGPSTS"
     gene            complement(10257..10562)
                     /locus_tag="PKJ61_09045"
     CDS             complement(10257..10562)
                     /locus_tag="PKJ61_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020386370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HOC14008.1"
                     /translation="MTVSEEELPAVAGAAHAVVREAKAAGVWVFGGGINESVPAVLVA
                     GDGSLTEGTYPQTRQIEGGYTVLEVPTREAALEWAAKIAGACRCAQEVREFMYDPES"
     gene            10844..11485
                     /locus_tag="PKJ61_09050"
     CDS             10844..11485
                     /locus_tag="PKJ61_09050"
                     /inference="COORDINATES: protein motif:HMM:NF016109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQ-loop domain-containing transporter"
                     /protein_id="HOC14009.1"
                     /translation="MTSVEVLGWVATVVGAVLATPQLVRLVRTRNVEGLSLVGWQTAL
                     VLNIAWTVHGINIGQLPQILSSSLALFTTVPILVLMTGVLGRRFVPTILPSLAVAAVM
                     IAVDLMLGSAAYGIVAIFPGIAITAAQSVELVRAEHVRGVSPVSLMLGFLNLTLWAVW
                     AFLVNDSGTMIAVSVSWVVAVFNLIWYWLRRLGLRSFFAPVAVSVPACVGPAD"
     gene            complement(11764..12123)
                     /locus_tag="PKJ61_09055"
     CDS             complement(11764..12123)
                     /locus_tag="PKJ61_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14010.1"
                     /translation="MPNHPPDAAVQADKADGQGIDLRVDRDRRHLLADPERMPPGLPA
                     GTTLRKLTSAGTFNLDNVRYLVDAQRGFDDVLVVADGDQIMVVDFNGEILLETTRPAP
                     GVRYVGRPRGVRWSPKS"
     gene            12315..12893
                     /locus_tag="PKJ61_09060"
     CDS             12315..12893
                     /locus_tag="PKJ61_09060"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOC14011.1"
                     /translation="MAATQTGYPVWVANDAMLGALAEFDFGAGRGRRSLVYLNGGTSG
                     IGGGVIIEGTLLKGRAGYAGEFGHVRVSSSPARDSAGFSGTLEAEVTREELCSVLGLG
                     RPGPQEFASALLADRSTKVREVVRRQLNYLGTAIAAAINVLTLPPSLEGVTITAAELG
                     ENLLSVGAAGLAFTDLLADPTIVRQDMAVAST"
BASE COUNT         2275 a         4099 c         4476 g         2255 t
ORIGIN      
        1 ggcctgaccg cgatcatctc ggtgaagctc gccgagccgc agttcgaggg ccagaccaag
       61 accaagctgg gcaacaccga ggcacgttca ttcgtgcaga aggtggtcaa cgatctgctc
      121 ggtgactggt tcgagaagaa cccggccgac ggcaaggaca tcgtccgcaa gagccaggcc
      181 gctgccagcg cccgggtagc tgcccgcaag gcccgcgacc tggcccgcag ccgcaagggc
      241 ctgctaaaca agggccagta cggcaagctc tccgactgct cgtccaccga gccgaaggag
      301 tgcgaggtgt tcatcgtcga gggcgactcc gccggcggct cggcgaaggg cggacgcgac
      361 ccccgcacgc aggcgatcct gccgatccgc ggcaagatcc tgaacgtcga gaaggcccgg
      421 ctggacaaga tcctgcagaa caaggaggtc gaggcgatct tcaacaccct gggcaccggt
      481 gtgcaggacg atttcgacgt tgacaagctg cgataccaca agatcgtgtt gatggcggac
      541 gcggacgtgg acggcgcgca catccgcacg ctgttgctca cgctgctgtt ccggttcatg
      601 aagccgctga tcaacgccgg ccacatctac ctggcgcagc cgccgctgtt ccggctgcgc
      661 tggtcgaacg ccccgcatga gctggcctac tccgacgagg agcgggaccg gctgcgggac
      721 accgggatcg aggccggcaa gaagctgccg agcgtgaacc cgatccagcg ctacaagggc
      781 ctcggcgaga tgaacgccga agacctgtgg gacaccacca tggatccgga caagcgctcg
      841 ctgttgcagg tgacgctgga ggacgccgcc gccgccgacc agatgttctc gatcctgatg
      901 ggtgaggacg tcgagcagcg ccgcagcttc attcagcgca acgccaagga cgtccgcttc
      961 ctcgacatct gacctttgac gatggctgag cttgacgaag ccccggacca cccttcgaca
     1021 agctcaggga tcgttccagt acgaacaact ctcgtaggag aacccgaatg accgacggcc
     1081 ccgacgaacc tactgacgtc gagctcgaaa ccgtccccga gaatgacgcc cttgcgccga
     1141 ccgaggcgaa gaccgaggag atcgacctac aggtcgagat ccagcggtcc tacctcgact
     1201 acgcgatgag cgtgatcgtg ggccgggcgc tgcccgacgt ccgcgacggg ctgaagccgg
     1261 tgcaccgccg cgtcctgtat gcgatgtacg acggcggcta ccgtcccgac cgcggttgga
     1321 acaagtgctc ccgcgtggtc ggcgaagtga tgggtctgta ccacccgcac ggcgactcgg
     1381 cgatctacga caccctggtg cggctggcgc agccgtgggt gatgcgcgcg ccgctggttt
     1441 ccgggcaggg caacttcggc tcgccgggta acgacccggc cgcggcgatg cggtacaccg
     1501 agtgcaagat ggcgccgctg tcgatggaga tggtccgcga catcaccgag gacaccgtcg
     1561 acttcaagcc gaactacgac aaccgcgaga tggagccgac cgtgctgccg gcgcggttcc
     1621 cgaacctgct ggtgaacggt tcgaccggca tcgcggtggg catggccacg aacatcccga
     1681 cgcacaacct gcgcgaggtc gcgtccgcgg tgcagtggtc gctggagcat cccgaagcgt
     1741 ccaaggaaga gctgctcgag gccgccatgg agcgggtgaa gggcccggac ttccccaacg
     1801 gcgcgctgat cgttggccgt aagggcatcg aggacgcgta ccgcaccggt cgcggctcgg
     1861 tgatcatgcg ggcggtcatc gacatcgaag aggacaagaa cggacgcacc cagctggtgg
     1921 tcaccgagct gccgcacatg tgcaacccgg acaatctcgc ggtgaagatc gccgagctgg
     1981 tcaacgcggg caagctgagc gggatcgccg acattcgcga cgactcgtcc gctcgtaccg
     2041 ggcagcgcct ggtgatcctg ctgaagcggg acgcccagcc gcgcgtggtg atgaacaacc
     2101 tgtacaagca cacccagctg caggacacgt tcggctgcaa catgctggcg ctggtggacg
     2161 aggtgccgcg gacgctgcgg ttggaccagt tcatcacgta ctgggtgcgg caccagattc
     2221 aggtgatcca gcggcggacg gcgttccggc tgaaggaggc cgaagcaaag gcgcacctgg
     2281 accgcggtct ggtgaaggcg ctgaaccggc tggacgaggt gctggcgctg atccgggctt
     2341 cccgcacggc ggacgcggcc cgcgagggcc tgaaggacct gctggacatc gacgacgcgc
     2401 aggcgctctt catcctcgac cagcagctgc gccggctggc ctcgatggaa atccagcaga
     2461 tcaacgagcg cctggcagcg cgtgagatcg cgatcgcgga cctgaaggac atcctggcca
     2521 agcccgagcg gcagcggcag atcgtcagca ccgagttggc cgagatcgtc gacaagtacg
     2581 gcgatgagcg gcggacgcgg atcatcgcgg ccgacggcga cctgtcggac gaggacttca
     2641 ttcccgacga cgacatggtg gtgacgatca cccgcggcgg gtacgcgaag cgcacccgca
     2701 ccgacctgta ccggacgcag aagcgtggcg gtaagggcgt ccgcggggcg acgcttcgag
     2761 ccgacgacga ggtcgaccac ctgttcgcga caacgaacca ccactggatc ctgttcttca
     2821 cgaacctggg ccgggtgtac cgcgcgaaag tgtggcagct gccggaggcc ggccgggacg
     2881 ccaagggcgg ccacgtggcc gggctgctga gcttcctgcc ggacgagcgg atcgccgagg
     2941 tactgacgct acgggcctac gacgatgcgc cgtacctgct gctggctacg aagaacggcc
     3001 tggtgaagaa gaccgagctg cgggcgtacg actcgccgcg gcaggccggc gtgatcgcga
     3061 ttaacttccg cgacgcggac gacgagctga tcggtgcctc gctgtgttcg ccggtcgacg
     3121 atgtgctgtt ggtgtcgaag aagggccagg cgatccggtt cgcggccgac gacgagcagt
     3181 tgcgtccgat ggggagggcg acgtccgggg tgaccggaat gaagttccgt ccgggggacg
     3241 cgctgttgag catgtcgatc atcggggcgg acatgcccga ggacgatcgg tacgtgttca
     3301 ccgtgaccga cggcggtttc gcgaagcgga cggcggtgag cgagtaccgc cagcagggcc
     3361 ggggtgggct cgggatcaag gcgatgaagc tgaacgagga ccgcggttcg ctggtcggcg
     3421 gcctggtggt gcaggagtcg gacgaggtga tcgcgatcaa ggtgagcggc cagatcacgc
     3481 gctcggcggt ggccgaggtg gccgcgaagg gccgggacac gatgggcgtg aagttcgtcg
     3541 gtgtggcaga cgacgacgcc gtttcggtga tcgcgatcaa cccggagaca gccgcggacg
     3601 acgacgagca cgctgtggtt gccgaagatg cggcgctgca cggtaccgtt gaacttcagg
     3661 agcccgaggt agcgaccact gccgaccaga cgacgcccga ggaggagcct ggtgactgac
     3721 cgcaaaggca ctgctgaccc gtatgcgggt ccggcggcga cgaatgggtc cgagtggagc
     3781 gtggagggct ggacggtgtc cgaggacggc ctgagcgaca cggtcgtccg gatgccgaag
     3841 atcaccgacg ttgactcgca gccgaccttc gtcgcgccgg ccgaccggcc ggatgagccg
     3901 gtcgatccgc tcggtgacgg cgccccggcg ctgccttcga tggcggtgac cgcgccagtg
     3961 ccgccggttc ctgcggcggt agcggttgct ccggtcccac cggttccggc cgctgcggct
     4021 gcagttcccg tgtccgcacc gaagccgaag aagactggac tacggcgcac gcgaaaggcg
     4081 cgactgcggt tgtcgcggtt ggatccctgg tcggtgatga agacgtcgtt catgttctcg
     4141 atcgcgttcg ggatcatgtt ctgggtggcc acctggctgc tgtggacggt gatcgtgcag
     4201 tcgggcctgt tcgaggcgat caacgcgcag atcctgaaca tcatccagaa cccgagcaac
     4261 acgaccacgt ttcgcatcga ggactacgtg aacaccaaca aggtgctcgg catcgccgcg
     4321 ttgctggccg tggtgaacgt ggtgatctcg accgcgctgg gcacgctgtt cgcgttcctc
     4381 tacaacctca gcgcgaacgt cctgggcggc ctggagttga cgctggctga agactgaggc
     4441 tgacgttctc cgagccagcc acgtgcccga gcctctccgg ggccggtcga agggttcccg
     4501 cacggctggt cgagcttgtc gagacccatt tgccacaaaa cgcactgtcg atgctcgata
     4561 tcggctttca gcgcgcgtcc cgcgcaagtt cagcgcattt tgtcctgcaa gcgcccccgt
     4621 ccgccctcag tttggcctgg acgccacgac tcaggtaacg ttctgccttg cgtgcaacgc
     4681 aggcgggcct atagctcaga cggttagagc gctgccctga taaggcagag gtcactggtt
     4741 caagtccagt taggcccacc cgtagtcaga gcccgaatca cttagtcaac ggtggttcgg
     4801 gctctccgct ttttactcgt cgtcggggtt cagccgcagg gtcatcgcca tgagccaatg
     4861 atgccaatct ggtcgcgagg gtccaccccc gtcgcgcccg gtgagtttcg tgccaacgaa
     4921 taggtccggc aatgcacatc gtgcggcggt ttgcaccggg tgtggctgtc cgagtggcct
     4981 tggcgctgca cgttgctgtt gcagttcgcg gcggcggtgg gcgatctcat cggtttcagg
     5041 tggttccttg ggcagagggt ccgtaagggg agcaacaaga gagctttcgg atttttcgaa
     5101 tgtgtctaca ctgggaacat gagcacccag acgacagacg cgacgactgt cctgccaccg
     5161 gcggacatgg aggcgatgct cgacctctct cgcttcctcg cgcacgtcac cgagccggcc
     5221 gcgctgctgg ggcccgacgg ccagacagtg ccgctgccga ttgaggccta ccaagtgctg
     5281 gtgaaggtgg tggagtcgat gcgagcgggt aaggcgatca cagtcgcccc ccttgatcag
     5341 cggctcacca cgcaggaggc agcggacttc ctgggcatca gccgaccgac cctcgtgaag
     5401 ctgctcgaac agggcgagat ctcctacgag cggccagcgg ccggccggca tcgcagggtg
     5461 cggctcagcg acgtcctggc ctaccaggtt cgcaagcgcc aagagcgccg cgtcacgctc
     5521 gatcagatga ccgccgacgc ggtagaggcg gggctctacg acgtggttcc cgactacagc
     5581 gaagccctgc gggcagcccg caaacgtcgg gccagctgaa ccgtggcccg ctacagcgcg
     5641 ttcctcgatg cgtgcgtgct cgtccctatc gcgctggccg acacgctgct tcgcctagca
     5701 gaggccgggc tataccggcc gctgtggagt gatcgagtac tggacgaggt gatcgacgcg
     5761 atcggaggga tccatccaga gctcgcctcg ggtcccgcgc ggtcgcgaac tgatgccatg
     5821 caatcctcct ttgagggtgc atgcgtcacg gactgggagg agttggttgc tggcatcgct
     5881 ctcccggatc ctgacgaccg ccacgtggtg gctgcggcgc tgcagggacg ggcagacatg
     5941 atcgtcaccg cgaacgtgcg ggactttccg agcgagatct tggaaccgat ggggctcgag
     6001 gtccagcatc ccgacgagtt cctgctcaac cagctcgatc tcgagccgga catcactatc
     6061 gccaccctgc atcgccaagc agatgcgacg cgacgtccgg caatcaccac gaaggtcttg
     6121 ctcgaacacc tatccaggtg cggggttccg gggttcgcgg caacggcggc tcaccaactg
     6181 tggcgggcga cctaagtggc aacgtctcac cattcgatag cgcacgtagg ttagtgcttg
     6241 gtggtgaccg gttgggggtc tgtgattcct tacccggtaa caaggtcgtg cagggcattg
     6301 tggagcctgt ccgatcgagc ggtgctggat gcggcgtcgg cagctacccg agggctactg
     6361 ggcctaggct tccccgatct caggggttcc cggcgttgcc ctattctggc gggcatgtcg
     6421 ttcgcgtacc ttcccgagac ccgcacccgc gaccaggtcg atgcgctgcc cggaatcacg
     6481 gtgctccagt tcggcaccga ttggtgtggc tactgccgcg cggccgagcc ggcgatcgcg
     6541 tccgtcctca gcgaagcgga cggtgtgcgc cacgtcctgg tcgaggacgg caagggccgt
     6601 ccgctgggac gctccttccg ggtgaagctg tggccgacgc tggtattcct cgccgacggt
     6661 gcggagatcg cccgggtggt ccgtccgtgg ggtacgggtg aactggtcga ggcgctggct
     6721 cgcgtccgcg ccgctgctga ggctgcataa cgccggcagg cgtccggcct ctgcccgcat
     6781 tcgtgcctct gcccgtattc atggggtaac ccaggatgac cggacgaacg cgcccacccg
     6841 ccgacgggga ccccggcgcc aacggggcag cggagatctc acgctaactc ctgatcggcg
     6901 cagattccat gttgcgtggt tgtccgcaag cggccttctg ctttcgttga cacccaccct
     6961 tgcccgccag ctagcctgat ttcacctacc gattggatcg tttcaacgaa gaggcgagat
     7021 ccctctgcga accatttcca atcagccggg aactagtttc ggatgattcc gcagggcagt
     7081 tcgttaggtg actttgtgct gctccaagaa tctccgtgga ccctttcgca gccacccgct
     7141 gcgcaatcgc taggaggtgc cgatcgccgg tctcgcgccc ggtgggccgg tggtgacgac
     7201 ggtgaagttc aaccagaacc agctgcggct ggtgaagaga aagaccttcc agctcgagga
     7261 gggcgtgtac tacaccaacg tgcaccccgc ctacgccgcg gcggtgacct ggggttcgtc
     7321 gaactcgaag atcgccacgg tgtccggtga cggcatcgtt aaggcgaaga agtcgggaac
     7381 ggtgacgatc acggctacca ccaacgagct gaccgaggcc ggcgcgaagg cgtccacctc
     7441 gatcaggatc acggtggtgg cgaagaagcc gaaggtcaag gtgaccaagg tgtccgcgtc
     7501 cgtcccgacc tcgctgaagc tggggcagtc ggcgtacatc accggcagct acgcctccgc
     7561 gaaggcggct ggggtcaagg tcagctacgc ctcgtccaac gacagcgtcg ccagcgttga
     7621 cgcggtcgga cggatcgtgg cgaagaacgc cggcaccctg cggatcacgg tgaaggcagg
     7681 cagcaagacc aggacctacc cggtctcggt cggctagcca tgatcgctgt gggggtgggt
     7741 cgctcggccc acccccacag ctctgcgcag cggaagccgg acgcgcagtc acgcatgcat
     7801 acgagccgtt tggctgaccc caacgcagcg cggtccgaag tgaacttcac agataccttg
     7861 catgcaatga tttggtgcac taacgtgagg atccgatgcg gattgaatcg ttctaacgct
     7921 gatctcagcc acgacggaag tagcagcggc cggtgagcag ccggtgatgg tccgagcaga
     7981 gtcgacgcga ggaaaggaga actcgatggc tgttggtttc gtcgtcgagc atgagcaatg
     8041 cgcggcccag actgatagct cgctagcggc tcttgccgag tctcgcgagg gcgtccgcac
     8101 ccaggatctt cggattctgc ggttcctggc tgaaggcctc accttcgatg cggtcgacgc
     8161 cgcctcggca tctcggcccg aaccgcacgc cggcgcatgc gggacctttg cgaggagtgg
     8221 caggtcggca cgacggttga ggcgatcgtg gaggccgttc ggaagggcct gatctagcga
     8281 ccaaggatcg ctagccgctc ggcgtgaccg cagtgaactt gccggcctcc gtgacgatac
     8341 gggaccttgc ctcttcgatg gtgagacgtt cggtgcttac cgatgcggcc agccaggcca
     8401 gcgactccag gtactccgag cccatgtcga agtcctggtc ctcgagcacc cactgcgtgc
     8461 tgtagccggt cgccagcgcc attcgagctc tggcgagcgc cgccggatcg acctctggac
     8521 gcagttcccc attgtccttg cagatctgat agcgcccggt gagcctcgag acgatgctgc
     8581 gatacatctc ggcgtgatcg gcgtgggtgc ggctgaatgg attggacccc tcagaggtgg
     8641 ccgccaccga gaactcgatc cagcgcggat tctcctgcga gagtgccttc gatcgccagg
     8701 ccaacccgag ctcttcgaag aaagtcagtg gagcttccag ctgggaccaa ctcgcgcgct
     8761 tctcgtccgc atgccgccgt gcgaccgcgc tgagcaggtg tgacttggac gggaagtagt
     8821 gcagcaggcc gccgtcggtg aggccgcagg cggccgcgat ctgccgaagg ctgctgttgt
     8881 gatagccctg ctcgaggaag acgcgaaaag cggtgtcgac gatgctggcg atcttctcgt
     8941 ccgatcccga gtaccgtccg cgtgcaccgt tcacgccgcg attgtaggtg gcaagggcag
     9001 ttggccgcgc acgtcagctc agcagcccgg cgagcaggtt cgcgatgacg tgagccagca
     9061 cgcccggcgc gacgctcgcg gtgtgttggc gcagccaccc gagcatcagg ccgatcacga
     9121 actgcccggc gaccagccag atcagcgacg ggctgacggt gagtagaagc aggtggggaa
     9181 gcaggaaggc gaccccttgc acgatgatgc cgacggaggc accccagtgc cgcgcgatga
     9241 tgccctgcag gaaccctctg aagaagagtt cctcgccggc ggccgcgagc acgaccgcga
     9301 gggcgcccca agcgttgacg atccgtccgg aggtaccgga accatgcagg acggcggccg
     9361 gcacgccaat ggtcgccgtc caagcgatcg cgatgctgat cagggcgagc gccgcgccgt
     9421 acagccaccc gctcaggttc ggccaacgca gacccaggac ttgcgcggcg tcccgcgtcc
     9481 ggccgcggac caggaagtag atcagtgacg gcagcgcgaa cagagcgaac tcgagcatgg
     9541 cctcattctc tcgctcctgc acgcgggtct cccgctgccg ggcggcgcga agcttggttt
     9601 gagtgagcgg ccccacgaaa tgggacattg ccgacaccac cggcgttctg agaacgcatc
     9661 cgacggctct cttgcggtct cgggggagct ctgtcccaat tcagatgccg cgagcaggac
     9721 caagatgctg attgcttgtc gctcgggttg cgcggacagt gagcgtagcc gcttccgccg
     9781 cgacgaagga tcgccatgga ccccatcgaa accaacccgg agctctaccg tgttgttcga
     9841 taacgaccgc gtccgggtgt tggaataccg ggacggcccc ggcgacgcca cccggccgca
     9901 cacccatccc gacagtgtga tgatcacgct gagcgcattc cggcgtcggc tccgttccgg
     9961 gggtcgggag acagacatcg agttgccggc ctttcaggcg cgttggttgg acggacagga
    10021 gcattccggg acaaacatcg gccagacccc cacgcacacg atcttcgtgg agttgaagga
    10081 gcccgccgag cgcggtgacg cccccgggcg gctggggcct tcgacgtcct gaccctcttt
    10141 gcactccggt tcacggcgtt cttcgaagtc cggaccatag ggtgtggctc catgcagcgc
    10201 ggacggtgtg ctggctcact gtctaggggc tcaactgcag gtgcggaact cgaccatcag
    10261 ctttccgggt cgtacatgaa ctcccggacc tcctgggcgc accggcaggc gcccgcgatc
    10321 ttggctgccc actccagcgc ggcctcccga gtcggcacct cgagaaccgt gtagccgccc
    10381 tcgatctgcc gggtctgcgg gtaggtgccc tcggtcaggc tgccgtcccc cgcgaccagg
    10441 accgccggga cgctctcgtt gatgccgccg ccgaacaccc agacgcccgc cgctttggcc
    10501 tcccgtacca cggcgtgcgc ggcgccggcg acggccggaa gttcttcctc ggagacggtc
    10561 atggcgccgc tggggaatga gatcagatac ttcgccgtcg gtcagatcct ctctgcgttt
    10621 ccagtgatcg caccatccgc gcggcccctc gctggcggac gggggcgcgg cggtcggtgt
    10681 gcagtgtcgg cggtacatcc cgacatggtg acgccggcgc gcggtaaccc ttaggctgag
    10741 tgcaaagcga tggtcgatga agcccgcagg caccgcggtt gggagcgctc tcaaccttgg
    10801 atagcgggag tagtggaagc gagacagagg gagttcggga cagatgacgt ccgtcgaggt
    10861 gctgggctgg gtcgcgactg tggtcggggc ggtgctggcg accccgcagt tggttcggct
    10921 cgtgcggacg cggaacgtcg agggtctctc gctggttggc tggcagaccg ccctggtgct
    10981 gaacatcgcc tggacggtgc atggcatcaa catcgggcaa ctgccgcaga ttctgagcag
    11041 ctcgcttgcg ctgttcacta cggtgccgat tctcgtcctg atgacgggtg tgctcggacg
    11101 gcggttcgtc ccgacgatcc tgcccagcct tgcggtggcg gccgtaatga tcgctgttga
    11161 cctgatgctg gggtcggccg cgtacggcat cgtggcgatc ttcccgggca tcgcgatcac
    11221 cgctgcccaa tcggtcgaac ttgtccgcgc cgagcatgtg cgcggcgtct ccccggtctc
    11281 gctgatgctc gggttcctca acctcacgct ctgggcagtc tgggcgttcc tggtgaacga
    11341 ctccggcacg atgatcgcgg tatcggtctc ctgggtcgtc gccgtcttca acctgatctg
    11401 gtactggctg cgccgactcg ggttgcggtc gttcttcgca ccggtcgcgg tttccgtccc
    11461 ggcgtgcgtg gggccggctg attagttctg gacgcgagcg atgcccgaac cggcagcatg
    11521 cccgcgtcgg gctgagccgg aggctacaag tactgacgga ctgtggaacg tagccgctcg
    11581 gcgtaccgat agatgtcgtc cacccgatcc aatggaatac gttcttcctt cttgtcttcg
    11641 tcgaacagtc ccagatactt ctgtgttcga ttgaagtgga ggcgacagac tggctttcgg
    11701 ttgtgtgatg tatcaggaca gcggtgacag ttctgcatca ggagaccggt gacggttggt
    11761 gtgtcaggac ttcggtgacc atcggacgcc gcgtggtcgt ccgacgtagc ggacgccggg
    11821 tgctgggcgg gtggtttcca ggaggatctc gccgttgaag tcgacgacca tgatctggtc
    11881 gccgtcggcg acgacgagaa cgtcgtcgaa gccgcgttgt gcgtcgacga ggtagcggac
    11941 gttgtcgagg ttgaaggtgc cggcgctggt cagtttccgc agtgttgtgc cggcgggcag
    12001 tccgggtggc atgcgttcgg ggtccgcgag caggtgtctg cgatcgcggt caaccctgag
    12061 atcgatgcct tgaccatcag ccttgtcggc ttggacggcg gcgtccggcg ggtgattcgg
    12121 catgaatacg atcggatccc gacagcgact gaggtagtcg ggatcacttc ggcagtgatc
    12181 gccggcatga ggatggagtt gcagggcctg ctggttactg gcattggggt cgcagtgcct
    12241 ggacaggtac gaaccaggga cggattggtg cggaacgcgc cgcatctcgg gtggcgtgac
    12301 gaaccgatcg ctcaattgct gccacccaga ccgggtatcc agtatgggtg gccaacgatg
    12361 cgatgctcgg cgcgctggcc gagttcgact tcggtgccgg ccgcggccgt cgtagcttgg
    12421 tctacctgaa tgggggaacc tcaggtatcg gcggcggcgt gatcattgag ggcacgctgc
    12481 ttaagggccg cgccggctac gcaggcgagt tcggtcacgt ccgggtgtcg agttcccccg
    12541 cccgggactc cgccggtttc tcaggaacac tggaggccga ggtcacccgt gaggagctgt
    12601 gctcggtcct ggggcttggg cgtccgggtc cccaggaatt cgcgtccgct ctcctcgccg
    12661 atcgctccac caaggtgcgt gaggtggtcc ggcggcaact caattacctg gggacggcga
    12721 tcgctgcagc gatcaatgtg ctcaccctgc cgccatcgct ggaaggggtc accatcactg
    12781 cagccgaact tggcgagaac ctactctcgg tcggtgccgc cgggttggct ttcactgatc
    12841 tattggcaga tccgacgatt gtgcgccagg acatggcggt cgcaagcacc tagaactggg
    12901 tagttctgcc tcggcacctc gaaagtctga acagtgcgtt tggccgcgct gggctgatcc
    12961 gatctccggc tcgaggccgc gctcgaaccg gtgtcgccag ccccgaaagc aactgagcga
    13021 tcagggcaaa gcgattcgcg gctgaatcac gtccgccaag ttcagccggc gagcaccaga
    13081 taggtgacga acgccgcgac cgaag
//
LOCUS       DAOPRQ010000065        12863 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_4098_length_12863_cov_14.151077, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000065 DAOPRQ010000000
VERSION     DAOPRQ010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 12863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12863
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4098_length_12863_cov_14.151077"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..845
                     /locus_tag="PKJ61_09065"
     CDS             <1..845
                     /locus_tag="PKJ61_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011758110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOC14012.1"
                     /translation="MNTTEVENYPGFTDGIMGPDLMLAMRAQAERFGAEIVTDDVVAV
                     ELTGEVKSVTDAEGNTYSARTVILAMGSGYRKLGLPAEEVYSGRGVSWCATCDGFFFR
                     DKDIAVVGGGDSALEEATFLTRFAKSVTLIHRRDELRGSKIMIDRAMADPKITVAWNS
                     VITDLHGNGSLAGITLTDTVTGATRELPAQGLFVAIGHDPRSEIVRGQVEVDASGYVL
                     VDAPSTRTNLAGVFACGDLVDHTYRQAITAAGTGCAAALDAERYLAALSDAAANVAPA
                     TASV"
     gene            856..1179
                     /gene="trxA"
                     /locus_tag="PKJ61_09070"
     CDS             856..1179
                     /gene="trxA"
                     /locus_tag="PKJ61_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013866150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOC14013.1"
                     /translation="MANVAEVTDATFAAQVLQSSRPVLVDYWADWCAPCKQLSPIIDE
                     LARDYGDKVTFLKLDTNVNTATPVNYGVLSLPTIQIWVGGEVVKSFTGAKPKSVLLKA
                     IDEYL"
     gene            complement(1320..2315)
                     /locus_tag="PKJ61_09075"
     CDS             complement(1320..2315)
                     /locus_tag="PKJ61_09075"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4325 domain-containing protein"
                     /protein_id="HOC14014.1"
                     /translation="MEPDLLPIGAASKLLGVSTSYLRKLTERGVLSSVRTPGGHRRYR
                     PETLHREWAAFRSEPAGPGVRWSQTYPLAGLAEDEVWADLRTAVEGQSGAIPPRARTI
                     LGYAVTEMVNNAIDHSGGAIVVVALRVSGRKLEVEIRDDGVGAFRRMASKFGYADPAD
                     AIVDLTKGKRTTAPDRHSGEGIFFTSKAVDVLSIEANGYQVSFDNLTPDIALGVGSDA
                     GTTVRLVLELDTPRELVDVFAAYTTAGEFDRTTPRIELVRVGGEFLSRSEAKRFAAGL
                     EKFDRVVLDFTGVQMVGQGFVDELFRVWQADHPGVRMAVTGANPAVALMLSRVAR"
     gene            complement(2489..3007)
                     /locus_tag="PKJ61_09080"
     CDS             complement(2489..3007)
                     /locus_tag="PKJ61_09080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14015.1"
                     /translation="MTLAELPELPCPYCGRVLGTDTPWAASAATLWGWCGARLVVGDE
                     VAGLLLVGPMEDATQAMVMSAWVSPDHVGVGHGRQLVQRVAAGLVEQRVRAVFAQGSN
                     THVRCSAPPRDFLRAVGFTRGLDERLWRLELDRTVADRRGVRSALERFMDSLRPVAPP
                     EPAGGAVRSELR"
     gene            3150..4466
                     /locus_tag="PKJ61_09085"
     CDS             3150..4466
                     /locus_tag="PKJ61_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014848081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOC14016.1"
                     /translation="MSLPEPSRRDSRLDPYIDAYAERTHGLTVSAVRALFAVANRPEV
                     VSLAGGMPNITDLPREALGRAVDRLISERGTQAMQYGSGQGEPFMREQIVEVMAEEHI
                     HAHADDIVVTCGSQQALDLVTRVFVDPGDVVLAEAPSYVGAIGTFRAYQAEIVHVSMD
                     EHGIEPDALRAALVSCKAAGKRVKFLYTIPNFQNPTGVTQSVERRRELLAIAKEHDLM
                     VVEDNPYGLLTLEAEPKPALRSMDDEHVIYLGSFSKIFAPGFRVGWVLAPHAVREKLV
                     LTQEAATLCPPVFSQFAIATYLHEFDWRGQIKVYREMYRERRDAMLAGLVEHMPPGTT
                     WTHPNGGFFVWVTLPEGLDSQAMLPRAISARVAYTTGTAFYADGLGSRNMRLSFCFPT
                     PERIIEGTRRLGEVLAQELEMHHTFGLGVDAPRHFDLTTGPAPDIS"
     gene            4476..5453
                     /locus_tag="PKJ61_09090"
     CDS             4476..5453
                     /locus_tag="PKJ61_09090"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020574625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HOC14017.1"
                     /translation="MTDQPTHGPIVVLAGGLSHERDVSLRSGRRVAQALRDQGREVIE
                     SDLNPDLIELLRGLQNPVAFPVLHGGVGEDGGLQQVLTLLDVAYVGSEPGACRRAFNK
                     AVAGPLIAQAGLARPRLAALPHDMFRELGAAALVAALGERIGFPMIVKPARSGSALGC
                     TKVNAAAELPSAMVGAYSYGDLAVIEEFVDGTEVAVTVIEQDGVPVALPAVEIRPNSG
                     IYDYTARYTAGETRFIVPAELGDEVADACAELAIAAHRVLGLRDLSRSDIVVRPDGTP
                     VFIEVNSAPGMTETSLLPQEVEAAGWDFGVLCGQLVDGAWARHVSERVG"
     gene            5463..6203
                     /locus_tag="PKJ61_09095"
     CDS             5463..6203
                     /locus_tag="PKJ61_09095"
                     /inference="COORDINATES: protein motif:HMM:NF014193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HOC14018.1"
                     /translation="MGVRTKQWLIAVLGAAITLTMLWLGLWQMRVFEDKENESAAHRA
                     MQPPVALLDYVTADGTVGDIYGKPASVVGRYLPEQQVAVVSEDGSVRVLSALELVDGR
                     VLPVVRGVLVAGSNELPAPPAGELTQTGIFLPSEAASDNEATVTGAETGGSPEALGSV
                     RLAALAQTWPQQLMPGFITLSASDSAAQGLDEATLSLPSGEGSIQNAGYALQWWAFAA
                     FAAFMTWRFVRTIGRQGTLGTLSNQEEE"
     gene            6200..6571
                     /locus_tag="PKJ61_09100"
     CDS             6200..6571
                     /locus_tag="PKJ61_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014598718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3817 domain-containing protein"
                     /protein_id="HOC14019.1"
                     /translation="MSQQETVTPAISPEQVPGIRGALLRYRIMAYLVGVLLVVLVLIG
                     MPLKYFVDNGSVVTWTGVPHGWLYMILLVTAYDLGRRVHWPWGRLLIIALAGTVPFLS
                     FWAEYSARNDVQARLRDAELR"
     assembly_gap    6576..6675
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6676..6960
                     /locus_tag="PKJ61_09105"
     CDS             <6676..6960
                     /locus_tag="PKJ61_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14020.1"
                     /translation="GCSGGSSAQQAPVPALDVSASSAAAEAPAAAPSESAAAVAAELA
                     FADTICAKPEMASLPGKTKSKANPTAGAVSVDGANIGNPVRPAWTRPLRR"
     gene            7164..7304
                     /locus_tag="PKJ61_09110"
     CDS             7164..7304
                     /locus_tag="PKJ61_09110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14021.1"
                     /translation="MDASHGAFVNTSEGLGKAEAGALYHAMEDSFLQFLKKWPDRQIS
                     IK"
     gene            complement(7306..8205)
                     /locus_tag="PKJ61_09115"
     CDS             complement(7306..8205)
                     /locus_tag="PKJ61_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016455016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOC14022.1"
                     /translation="MNANRHGLGRGLGDLLQRTDPELAPQPAEGDVEPPAGARFGEIA
                     VADIRPNPKQPRTVFDEDALAELVGSIQEVGLLQPVVVRPLDDGKYELVMGERRLRAT
                     QQAGLATIPAIIRRTEEADLLRDALLENLHRAQLNPLEEAAAYQQMLSDFDCTQDELS
                     QRIKRSRPQISNTIRLLQLPASVQRRVAAGVVSAGHARAILMLSDQAAMEHLATRIVA
                     EGLSVRATEEIVSVGEQGTKRAPRSRRPVELSSAADELQRVLTERLDTRVLVSEGRSK
                     GRIVIEFAGIEDLERLSDLLLNN"
     gene            complement(8202..9128)
                     /locus_tag="PKJ61_09120"
     CDS             complement(8202..9128)
                     /locus_tag="PKJ61_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016668685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HOC14023.1"
                     /translation="MTITPPPTPRRVTFDEPEFGDPHTGEPDDLELDVSRETTLPKPD
                     KTRVLVVANQKGGVGKTTTSVNLATALALGGLTVLVVDLDPQGNASTALGIEHHEGVQ
                     GTYEVLTANSPIETLIKRSPESQGLWVLPATIDLAGAEIGLVSTHARESRLLHALRKF
                     NDKYHPDYVFIDCPPSLGLLTLNALVAAEEILIPIQCEYYALEGVSQLLRTINLVKGS
                     MNDHLYLSTVLLTMYDGRTRLASQVAEEVRRHFPNETLRAAIPRSVRVSEAPSYGQSV
                     ITYHAASAGAQAYLDAATELAERGVARKENQE"
     gene            complement(9709..10311)
                     /gene="rsmG"
                     /locus_tag="PKJ61_09125"
     CDS             complement(9709..10311)
                     /gene="rsmG"
                     /locus_tag="PKJ61_09125"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016666585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HOC14024.1"
                     /translation="MDVRAELFGDGAEVIRQYVDILSSRGIDWGLLGPREGERLWDRH
                     VLNSIACADLLAAGSSVVDVGSGAGLPGLPLAMLRPDLQVTLLESLLRRANFLELVVA
                     ELGLGERVAVIRSRAEDHDGRYDAVVSRAVAPLPRLVGWCLPLMRPDGCVLALKGSTA
                     SDELTDAAPLLRKLRLVGEVHELAVPGTAETTWAIELRQS"
     gene            complement(10317..10940)
                     /locus_tag="PKJ61_09130"
     CDS             complement(10317..10940)
                     /locus_tag="PKJ61_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015072078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HOC14025.1"
                     /translation="MSKETIVSEDQIEASPEVAADVEAVTESDQVADVAADSESEPAA
                     DEKADGSTRSKKETALENEGEIAADYLEELLDIADLDGDIDTYAEGGRAHISILTEGD
                     VLVGKNGEVLEALQELARLSVMTETGHRSRLMLDIGGHREKRRKVLQDLAADAVAEVK
                     DIGEPVRLAPMNPFERKIVHDAVAAAGLVSESEGEEPQRRVVVLPKD"
     gene            complement(10940..12034)
                     /gene="yidC"
                     /locus_tag="PKJ61_09135"
     CDS             complement(10940..12034)
                     /gene="yidC"
                     /locus_tag="PKJ61_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014848090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HOC14026.1"
                     /translation="MPLLVPLSLWDDFLGLLGAMMTPLYWAVSGILVGFHSLLTMLGM
                     NADWGGTWALSIVLLTVMIRTALIPLFVRQINSSRNMQLLQPKVKELNEKWGHDRERL
                     GQETMKLYKDEGVNPMASCLPLLLQMPIFLALFYVLNSVSHGNALGYFFETNPHLVTS
                     LQSATIFGAPISSTFLPFTTFGPTQFVTLTLIILMVGVLFITQLQLMRKNMPPEALTG
                     QMAQQQKMMLYLFPGIYAIGGVSIPIGVLIYWLTSNLWTMGQQYILIHNNPAPGTPAY
                     IDWEERMRAKGLDPDEIMAKRRGGKKPAATAPSDPTKVARQGRSGAPAAKAPAGPSEA
                     STNMDAAAGVQRQVIQRQQPKKNSRAARKQ"
     gene            complement(12040..12366)
                     /gene="yidD"
                     /locus_tag="PKJ61_09140"
     CDS             complement(12040..12366)
                     /gene="yidD"
                     /locus_tag="PKJ61_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013162246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOC14027.1"
                     /translation="MKYVVIGLLKGYRKVISPLYGNVCKYYPSCSAYALEAVTVHGAA
                     KGTWLAARRLGSCHPWAAGGYDPVPGTEAARVWAAEQAAKAAANANPAEPAPTGTHLA
                     SVRCEK"
     gene            complement(12363..12698)
                     /gene="rnpA"
                     /locus_tag="PKJ61_09145"
     CDS             complement(12363..12698)
                     /gene="rnpA"
                     /locus_tag="PKJ61_09145"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016668691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOC14028.1"
                     /translation="MLPARSRLRNPTDFRDTLRQGVRSGRASLVVHARRAALPESRAG
                     FIVSKAVGNAVSRNRVKRRLRHLAAAALPSAPFGVDVVVRALPPATTADLAGDFNSAF
                     AACLDRLAA"
     gene            complement(12711..12848)
                     /gene="rpmH"
                     /locus_tag="PKJ61_09150"
     CDS             complement(12711..12848)
                     /gene="rpmH"
                     /locus_tag="PKJ61_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013428281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOC14029.1"
                     /translation="MSKRTFQPSNRRRSRTHGFRLRMRTRAGRAIISARRRQGRVRLA
                     G"
BASE COUNT         2111 a         4272 c         4205 g         2175 t
ORIGIN      
        1 tgatgaacac caccgaggtg gagaactacc ccggcttcac cgacggcatc atgggtcccg
       61 acctgatgct cgcgatgcgc gcccaggccg agcggttcgg cgccgagatc gtcaccgacg
      121 acgtcgtcgc ggtcgaactg accggcgagg tgaagtccgt caccgacgcc gaaggcaaca
      181 cctactcggc gcgcaccgtg atcctggcca tgggctcggg ctaccgcaag ctcggcctgc
      241 ccgccgagga ggtctacagc ggacgcggcg tgtcgtggtg cgcgacctgc gacggcttct
      301 tcttccgcga caaggacatc gccgtggtcg gcggcggcga ttccgcgctc gaagaggcca
      361 cctttctcac ccgcttcgcc aagtcggtca cgctcatcca tcgacgcgac gagttgcgcg
      421 gctccaagat catgatcgac cgcgccatgg ccgacccgaa gatcaccgtc gcctggaact
      481 cggtgatcac cgacctccac gggaacgggt cgctggccgg catcaccctc actgacacgg
      541 tcactggcgc cacccgcgag ttgcccgccc agggcctctt cgtcgcgatc ggacacgacc
      601 cgcgctcgga gatcgtccgc ggccaggttg aggtggacgc gtccggctat gtcctggtcg
      661 acgcgccgag cacccgtacg aacctggccg gagtgttcgc gtgcggcgac ctcgtcgacc
      721 acacctaccg ccaggcgatc accgctgccg ggacgggttg cgccgccgcg ctggacgcag
      781 agcgctacct tgccgcactg tcggacgcgg ccgctaacgt cgctcccgct accgcttccg
      841 tctaggagaa cgaatatggc caatgtcgcc gaagtcaccg atgccacctt tgccgcgcag
      901 gtgctgcagt cgtccaggcc agtgctggtc gactactggg ccgactggtg cgcgccgtgc
      961 aagcaactgt ccccgatcat cgacgaactg gctcgtgact acggcgacaa ggtcactttc
     1021 ctcaagctcg acaccaatgt gaacaccgcc accccggtga actacggcgt gctgagcctg
     1081 ccgacgatcc agatctgggt tggcggcgaa gtggtgaagt cgttcaccgg agccaaaccc
     1141 aagtcagtgc tgctgaaagc cattgacgag tacctgtagt cgactggtcg gcaccttctg
     1201 agatcccggc ctacgccggg atgaccttgg gctcaggctg agcagctagc gcaagcggtg
     1261 gagaccggtc cctgaggagc tcggcgcgag gaacgagcgc cgagcgtcac gaagggccct
     1321 catcgagcca cccgactaag catcagggca accgccgggt tggctccggt cacagccatg
     1381 cgcacgccgg ggtgatcggc ctgccacact cggaacaact cgtcgacaaa cccctgcccc
     1441 accatctgaa cgcccgtgaa atcgagcacc actcggtcaa acttctccaa gcctgcggcg
     1501 aagcgcttcg cctcgctgcg gctgagaaac tccccgccta cccgcaccag ctcgatcctg
     1561 ggtgtggtgc gatcgaactc gcctgcggtg gtgtaggccg cgaagacgtc gaccaactca
     1621 cgtggggtgt ccagctcaag gaccagtcgg acggtggtgc cagcgtccga accgaccccc
     1681 agcgctatgt ccggagtcag gttgtcgaag ctgacctggt agccgttcgc ttcgatactc
     1741 agaacgtcca cagcctttga agtgaagaag atcccttcgc cggagtgtcg gtctggagcc
     1801 gtggtccgtt tgcccttggt gaggtcgacg atcgcgtccg ccggatcggc atacccgaac
     1861 ttgctcgcca tcctgcgaaa agctcctacc ccgtcgtccc tgatctcgac ctcaagcttg
     1921 cgaccggaga cccgcagcgc gaccacgaca atggcgccgc ccgaatggtc gatcgcgtta
     1981 ttgaccatct cagtgacggc gtagccgagg atcgtcctgg ctcgcggcgg aatcgctcca
     2041 gattgacctt cgacagcggt gcgaagatcg gcccacacct catcttcggc gagccctgcg
     2101 agcgggtagg tctggctcca gcgcactccg ggacctgctg gctccgagcg gaacgcggcc
     2161 cactcccgat ggagcgtctc cggccggtag cggcggtgcc cgccgggcgt gcggacgctc
     2221 gacaggacgc ctcgctcggt gagcttgcgc aggtaactgg tggatacgcc cagcagcttg
     2281 ctcgccgccc cgatcggaag cagatccggc tccatgagca ccaaatctac accagtgtag
     2341 attcatcgga aagaaatcta cactcaagcc ggagattcga gccaggcggc gacccttcgt
     2401 gacgactcgc tcgttcctcg cgagtctcct cagggatcgt tttgtggtga actcgttcct
     2461 cgcgaacgct cctcaggacc gtttggggtc agcgcaactc tgagcggacg gctccgccgg
     2521 ccggctcggg cggggccacc ggacgcagcg agtccatgaa ccgctccagg gcgctgcgca
     2581 cgccgcggcg gtcggctacc gtccggtcga gctccaggcg ccacaggcgc tcgtccaggc
     2641 cgcgggtgaa acccaccgca cgcaggaaat cccgcggtgg cgcgctgcag cggacgtgcg
     2701 tgttcgagcc ctgcgcgaac accgcgcgca cccgctgctc gaccagaccg gcggcaaccc
     2761 gctgcaccag ttgccggccg tgcccaaccc cgacgtggtc aggcgatacc caggcagaca
     2821 tcaccatcgc ctgagtcgcg tcttccatcg gcccgaccag caacaacccc gccacctcgt
     2881 caccgaccac gagcctcgcc ccacaccagc cccacagcgt ggccgccgac gcagcccaag
     2941 gcgtgtccgt gccgagcacg cgcccgcagt agggacacgg cagctcaggc agctcggcca
     3001 acgtcaccat cgacagtctc ggcacttcac ccccactgct ccattcccgc cgctgaggct
     3061 tagtcttttc ctcaagcggt ggagtccatg tgaccaccgt gtgagtgggt cggtttcatc
     3121 ctaagcggtc ggtacggcag gctgtgaggg tgagcctgcc tgaaccgtcc cgccgcgact
     3181 cgcggctgga cccctacatt gacgcgtacg ccgagcgaac ccatggcctg acggtctccg
     3241 ccgtccgagc tttgttcgcc gtcgccaacc gtcccgaggt ggtctcactg gccggcggca
     3301 tgccgaacat caccgacctc ccccgcgaag cgctcggacg cgccgtcgac cggctgatca
     3361 gcgagcgcgg cacccaggcg atgcagtatg gctccggaca aggcgagccg ttcatgcgcg
     3421 agcagatcgt cgaggtgatg gccgaggagc acatccacgc tcacgccgac gacatcgtgg
     3481 tcacctgcgg ttcgcagcag gctctcgacc tggtcacccg cgtcttcgtc gatccgggcg
     3541 acgtcgtgct ggctgaggcc cccagctacg tcggcgcgat cggcacgttc cgcgcgtacc
     3601 aggccgagat cgtccacgtc agcatggacg agcacggcat cgagccggac gcgctgcgcg
     3661 ccgcgctggt cagctgcaag gcggccggca agcgggtgaa gttcctgtac acgatcccga
     3721 acttccagaa cccgaccggc gtcacccagt ccgtcgagcg ccgccgcgag ctgctggcga
     3781 tcgccaagga gcacgacctg atggtcgtcg aggacaatcc gtacggcctg ctcaccctcg
     3841 aagccgagcc gaagcccgcg ctgcgctcga tggacgacga gcacgtgatc tacctcggct
     3901 cgttctcgaa gatcttcgcg ccgggcttcc gggtgggctg ggtgctggcc ccgcacgccg
     3961 tccgcgagaa gctggtgctc acccaggaag cggccacgct gtgcccgccg gtgttcagcc
     4021 agttcgccat cgccacctac ctgcacgagt tcgattggcg tggtcagatc aaggtttacc
     4081 gggagatgta ccgcgaacgc cgggacgcga tgctggccgg cctggtcgag cacatgccac
     4141 ccggcacgac ctggacgcat cccaacggcg gcttcttcgt ctgggtgacg ctgcccgagg
     4201 gcctcgattc gcaggccatg ctgccgcggg cgatcagcgc ccgggtggcc tacaccaccg
     4261 gcaccgcctt ctacgcggac ggcctgggta gccgcaacat gcggctctcg ttctgcttcc
     4321 cgacgcccga gcgcatcatc gaaggcaccc gccggctggg cgaggtgctc gcgcaggaac
     4381 tcgagatgca ccacaccttc ggcctcggcg tggacgcccc ccgccacttc gacctcacca
     4441 ccgggccggc gcccgacatc agttagggat cagctatgac agaccaaccg acccatggcc
     4501 cgatcgtcgt gctcgctggc gggctctccc acgagcgcga cgtgtcgctg cgctccggac
     4561 gccgggtcgc ccaggcactg cgtgatcagg gccgcgaggt gatcgagtcc gacctcaacc
     4621 ccgatctgat cgagttgctg cgcggcctgc agaacccggt ggcgttcccc gtcctgcacg
     4681 gcggcgtcgg cgaggacggc ggtctgcagc aggtgctgac cctgctggac gtcgcctacg
     4741 tcggctccga gccgggtgcg tgccggcggg cgttcaacaa ggctgtcgcc ggtccgctga
     4801 tcgcacaggc cggactggcc cggccccggc tggctgcgtt gccgcacgac atgttccgcg
     4861 agctcggcgc ggccgcgctg gtggccgccc tgggtgagcg gatcgggttc ccgatgatcg
     4921 ttaagccggc gcgcagcggt tcggcgctgg gttgcacgaa ggtgaacgcg gcggccgaac
     4981 tcccgtccgc gatggtcggg gcgtactcgt acggtgacct ggcggtgatc gaggagttcg
     5041 tggacggaac cgaggtcgcg gtcactgtga tcgagcagga cggggtgccg gtcgcgctgc
     5101 ccgcggtcga gatccggccg aactcgggga tttacgacta caccgcgcgc tacaccgccg
     5161 gcgagacccg gttcatcgtc ccggccgagt tgggcgacga agtagcggac gcgtgcgccg
     5221 aactggcgat cgccgcgcac cgcgtcctcg ggctgcggga cctgtcccgc tcggacatcg
     5281 tcgtgcgtcc ggacggcact ccggtcttca tcgaggtgaa ctccgcaccg ggcatgaccg
     5341 agacgtcgct gctgccgcag gaagtcgagg ccgccggctg ggacttcggc gtgctctgcg
     5401 ggcagctcgt cgacggtgcc tgggcgcgcc acgtgagcga gcgcgtcggc tagcctgccc
     5461 atgtgggagt gcgcaccaag cagtggctga tcgccgtcct tggggccgcg atcacgctga
     5521 ccatgctctg gctgggcctg tggcagatgc gcgtgttcga ggacaaggag aacgagtcgg
     5581 ccgcgcaccg ggcgatgcag ccgccggtcg cgctgcttga ctacgtgacc gcggacggga
     5641 ccgtgggcga catctacggc aaacctgcct ccgttgtcgg acggtatcta cctgagcagc
     5701 aggtcgcggt ggtgtccgag gatggctcgg ttcgggtgtt gagcgctcta gaactggtgg
     5761 acggacgggt gctgccggtg gtgcgcggcg tgctggttgc cggttcgaac gaactgcccg
     5821 cgccgccggc tggtgagctg acccagaccg ggattttcct gccgtccgag gctgcgtctg
     5881 acaacgaagc gacggtaact ggagcagaga ccggcggatc acccgaggcg ctcggctccg
     5941 tccgtctggc ggcgttggcg cagacctggc cgcaacagct gatgccgggg tttatcacct
     6001 tgtcagcgtc ggattccgcg gctcagggcc tcgacgaggc cacgctaagc ctcccgtccg
     6061 gcgaaggttc gattcagaac gccggctacg cgctgcagtg gtgggcgttc gcggcgttcg
     6121 cggctttcat gacgtggcgc ttcgtgcgca cgatcggacg ccagggaacc ctcggtacgc
     6181 tgtcaaacca ggaggaagaa tgagccagca agagacggtg acccccgcga tcagccccga
     6241 gcaggtgccc gggattcgag gcgcgctgct gcgctatcgg atcatggcct acctggtcgg
     6301 agtgctgttg gtggtgctcg tgctgatcgg aatgccactg aagtacttcg tagacaacgg
     6361 ctccgtggtg acctggaccg gtgtgccgca tggctggctc tacatgattc tgctggtgac
     6421 cgcctacgac ctcggccgcc gcgtccactg gccctggggg cgcctgctga tcatcgcctt
     6481 ggccggaacc gtcccattcc tttccttctg ggccgagtac tcagcccgca atgatgtcca
     6541 ggcccgattg cgcgacgccg agctccgcta acggtnnnnn nnnnnnnnnn nnnnnnnnnn
     6601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6661 nnnnnnnnnn nnnnngggtg ctcgggcggt tcgtctgccc aacaggcgcc ggttccggcg
     6721 ctcgacgtga gcgcttccag tgccgccgct gaagctccag ccgctgcacc atctgagtct
     6781 gctgcagcgg tcgccgccga gcttgcgttc gctgacacga tctgcgccaa gcccgaaatg
     6841 gcctcactgc cgggcaagac gaagtcgaag gcgaacccga ccgcgggcgc ggtcagcgtc
     6901 gacggggcca acatcgggaa ccctgttcga ccggcctgga cgcggccact acggcgctga
     6961 agtccaggcc gcccgtccca tggggccgcg cctccgagac cggtctggcc tgtgtctgga
     7021 gcacggacac gtcgtcgctt tccttctctg gattcgccac cgaggacagc gaagtgaagg
     7081 atgtgtggga cagcgacgaa ttggtctctg gcgtggggaa gagcgctgtc tggaccaagt
     7141 ggagtagtca gcctcaggtt gagatggacg cgtcccatgg cgctttcgtc aacacctccg
     7201 aaggcctcgg gaaggccgag gccggcgcgc tctaccacgc gatggaggat tcgttcctcc
     7261 agttcttgaa gaagtggccg gatcgccaaa tatcgataaa gtgatttagt tgtttagcag
     7321 taaatcactt agtctctcaa gatcttcgat accggcgaac tcgattacga tccggccctt
     7381 gctgcgtcct tcactgacca ggactcgggt gtcgaggcgc tctgtcagta cacgctgcaa
     7441 ctcgtccgct gccgaggaga gttctaccgg acgcctggag cgcggcgcac gtttggtgcc
     7501 ttgctccccg accgacacga tctcctcggt ggcccgcacc gaaagccctt cagcgacgat
     7561 gcgggtggcc aggtgttcca tggcggcctg gtcggacagc atcaggattg ctcgtgcgtg
     7621 cccggcgctg accacaccgg ccgccacccg gcgctgcacc gacgccggca gctggagcag
     7681 cctgatcgtg ttcgagattt gcggacgtga gcgcttgatt cgctgcgaca gctcatcctg
     7741 ggtgcaatcg aagtcgctga gcatctgctg gtaagcggcg gcttcctcca gcgggttgag
     7801 ttgcgcacgg tgcaggttct ccagcagcgc gtcccggagc aggtcggcct cctcggtgcg
     7861 gcggatgatg gccgggatcg tggcgaggcc ggcctgctgg gtcgcgcgaa gccggcgctc
     7921 gcccatgacc agctcgtact tgccgtcatc gaggggacgc acgaccaccg gctgtagcag
     7981 cccgacttct tgaatcgagc cgaccagttc agccagtgcg tcctcgtcga agacggtccg
     8041 cggctgcttc gggttggggc gaatgtccgc gacagcgatc tcgccgaatc gcgcaccggc
     8101 ggggggctcg acgtccccct ccgctggttg cggggcgagt tcagggtccg ttcgctgcag
     8161 caggtcacca agtcccctgc ccaatccatg gcggttggcg ttcattcctg gttctccttc
     8221 cgcgcgaccc cgcgctcggc aagttcagta gccgcgtcca gataggcctg ggcaccggcg
     8281 gacgcagcgt gataggtgat gaccgactgt ccgtagctgg gtgcctctga tacgcgaact
     8341 gagcgcggga tcgccgcacg caatgtctca ttcgggaagt gccggcgcac ctcttcggcg
     8401 acctgggacg cgagccgcgt ccgtccgtcg tacatggtca gcaagacggt ggagaggtag
     8461 agatggtcgt tcattgagcc cttgaccagg ttgatcgtcc gcagtaactg cgatacgcct
     8521 tcgagcgcgt agtactcgca ctggatcggg atgaggatct cttccgcagc gaccagcgcg
     8581 ttcagggtga ggaggccgag cgagggcgga cagtcgatga agacgtagtc ggggtggtac
     8641 ttgtcgttga acttccgcag ggcgtgcaag agtcggcttt cgcgagcgtg cgtgctgacc
     8701 aagccgatct cggcacctgc gagatcgatg gtggccggaa gcacccagag tccttgcgac
     8761 tcgggtgagc gcttgatcag ggtttcgatg ggcgagttgg cagtgagtac ctcgtaagtg
     8821 ccctggacgc cctcgtggtg ctcgatcccc agtgcagtcg aagcgtttcc ctgcgggtcg
     8881 aggtcgacga ccagcacggt aagaccgccg agggctagtg cggtggcgag attgaccgag
     8941 gtggtggtct tgccgacgcc gcccttctgg ttggcgacga ccaagacgcg ggtcttgtca
     9001 ggcttgggga gcgttgtttc acgtgaaaca tccagctcga ggtcgtccgg ttcgccagtg
     9061 tgagggtcac cgaactcggg ctcgtcgaag gtgacgcgcc gcggagtagg cggcggggtg
     9121 atcgtcattg cgctcctgca tcaagtcgtt tcacgtgaaa cagcagccta acggttgggt
     9181 gtgacatcca tcgaagcagg aggcggccct gcgctcgctc ctgcgagcct agcccgcgtc
     9241 gttcacctct gcacggtcgg ttggggaggc cgagcacagg ccccttgttg tgggatggct
     9301 cttcagctct cgtcgctgca gcgagggttc ttaagggata gggaatgtgc tcggcctccc
     9361 caaccgacct tgaaagtccg gtccttggcc gcttgcgcct caagcgaaac accagcgtcc
     9421 gtgatgcgtt gacccaggcg gcgtaagacc ggatcgctga gcagcgtgct ggcaacactg
     9481 ggggttccct tgttcccggc atctcgacga cgaggaccgg gagtggctgg aactgctcct
     9541 cgggaacgct ggtagaacta ctggctgccc ggtggatgga gatcccgggt cgagcccggg
     9601 atgacggaat ggccttcatg gctgacggat gggtgcaagg caactgtaag cagactggac
     9661 gtttcaggtc ggtcggggag accgcgcaga cgtgcgctgg gacagcgctc aggactgacg
     9721 cagctcgatc gcccaagtgg tctcggcggt gcccggcacg gcgagctcgt gcacctcgcc
     9781 aactaggcgg agcttgcgca gcagcggcgc ggcatcggtg agttcatcgg atgcggtcga
     9841 acccttgagt gcaaggacgc aaccgtccgg tcgcatcagg ggcagacacc agcccaccaa
     9901 gcggggaagc ggggccacgg ctcgggagac cactgcgtca tagcgtccgt cgtgatcctc
     9961 agcgcgcgac cgaatgaccg caacccgctc tcccaggccc agctcggcga cgaccaactc
    10021 caggaagttg gctcgccgca gcagggactc cagcagggtc acctgcaggt cgggccgcag
    10081 catggccaac ggcagaccag gcagaccagc gccagaaccg acgtccacca ccgatgagcc
    10141 ggccgccaac aagtctgcac aggcgatcga gttcagcacg tgccgatccc agagccgttc
    10201 accctcgcgc gggccgagca aaccccagtc gatacctcga ctagacaata tgtctacata
    10261 ttgcctaatc acctcagcgc cgtcgccgaa cagctcagcg cgaacgtcca atcgactcag
    10321 tccttcggca gcaccacgac gcgacgctgc ggctcttcac cctcagactc gctgaccagg
    10381 ccagcggcgg ccaccgcgtc gtgcacgatc ttgcgctcga acgggttcat cggcgcgagc
    10441 cgcacgggct ctccaatgtc cttgacctcg gccaccgcgt ccgccgcgag atcctgcagc
    10501 accttgcggc gcttctctcg gtgaccaccg atgtcgagca tcagccggct gcggtgcccc
    10561 gtctcggtca tcacggacag gcgcgcgagc tcctgcagcg cctccagaac ctcgccgttc
    10621 ttgccgacca gcacgtcccc ctcggtgagg atcgagatgt gcgcacggcc gccctcggcg
    10681 taggtgtcga tgtcgccgtc caggtcggcg atgtcgagca gttcctcgag gtagtccgcc
    10741 gcgatctcgc cctcgttctc cagggcggtc tccttcttcg accgggtgga gccgtcggcc
    10801 ttctcgtcgg ccgccggttc cgattccgag tccgcggcca cgtccgcgac ctggtccgac
    10861 tcagtcaccg cctcaacgtc ggcagctact tccgggctcg cttcgatttg atcttcgctc
    10921 acgattgtct ccttgctcat cactgcttgc gggcggcgcg cgagttcttc ttgggctgct
    10981 gccgctggat cacctgacgc tgcacgccgg ccgcggcgtc catgttggta ctcgcctcgg
    11041 acggacccgc gggagcctta gcggccggcg caccggagcg cccctggcgc gcgaccttgg
    11101 tcgggtcgga cggagcggtc gcggccggct tcttcccgcc gcgccgcttg gccatgatct
    11161 cgtccgggtc gaggcccttg gcgcgcatcc gctcttccca gtcgatgtac gccggggtgc
    11221 cgggggccgg gttgttgtgg atcaggatgt actgctgtcc catcgtccac aggttcgagg
    11281 taagccagta gatcagtacc ccgatcggga tgctcacgcc accgatcgcg tagatgccgg
    11341 ggaacaggta cagcatcatc ttctgctgct gcgccatctg gccggtcagc gcctcgggcg
    11401 gcatgttctt gcgcatcagc tgcaactgag tgatgaacag cacgcccacc atcaagatga
    11461 tcagcgtcag cgtgacgaac tgggtcgggc cgaaggtcgt gaacggcagg aaggtggacg
    11521 agatcggggc gccgaagatc gtggcgctct gcagcgaggt caccaggtgc gggttcgtct
    11581 cgaagaagta ccccagcgcg ttgccgtgcg agacgctgtt gagcacgtag aaaagagcca
    11641 ggaagatcgg catctgcagc agcagcggca ggcaggacgc catcgggttc acaccctcgt
    11701 ccttgtacag cttcatcgtc tcctggccga gccgctcgcg gtcgtggccc cacttctcgt
    11761 tgagctcctt gaccttcggc tgcagcaact gcatgttccg cgagctgttg atctggcgga
    11821 cgaacaacgg aatcagcgcc gtgcggatca tcacggtgag cagcacgatc gacagggccc
    11881 aggtgccacc ccagtcggca ttcatgccga gcatggtcag cagtgaatgg aagccgacca
    11941 ggatgccgga gaccgcccag taaagcggcg tcatcatcgc gccgagcagg cccaggaagt
    12001 cgtcccagag actcaggggc accagcaggg gcagtaggtt cacttctcac acctcacgga
    12061 cgctaggtgc gtcccggtcg gggccggttc ggccgggttg gcgttggcgg ccgctttggc
    12121 cgcttgttcc gcggcccaaa cccgtgcagc ttcggtgccg ggaactgggt cgtaaccgcc
    12181 tgctgcccac gggtggcaac tgcccagccg acgagcggcc agccaggtgc ccttcgctgc
    12241 gccgtggacg gtaaccgcct ccagcgcgta ggccgagcag gacgggtagt acttgcagac
    12301 gttgccgtac agcgggctga tcacctttcg gtagcccttc aacagaccga tgactacgta
    12361 cttcatgccg ccaaccgatc caggcaggcg gcgaaggccg aattgaagtc accggcgagg
    12421 tcagcggtgg tggccggtgg cagggcgcgc acgaccacgt ccacaccgaa aggtgcggac
    12481 ggcagcgcgg ccgcggcaag gtgccggagc cgacgcttga cccggttgcg gctcactgcg
    12541 ttcccgacgg ccttggacac gatgaagccc gcccgcgact ccggaagagc cgcacgacgg
    12601 gcgtgcacga ctaggctcgc gcgtccactg cggacgccct gacgtagcgt gtcgcggaag
    12661 tccgtggggt tacgcaaccg cgaccgggca ggcaacaccg cgttgccggc tcagccggcc
    12721 aggcggacgc ggccctgacg gcgacgagcc gaaatgattg cgcgccccgc acgggtacgc
    12781 atccgcagac ggaagccatg ggtgcggctg cgacgccggt tgctcggctg gaaagtgcgc
    12841 ttgctcacgc gagtgctccc aat
//
LOCUS       DAOPRQ010000066        12730 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_4154_length_12730_cov_13.377199, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000066 DAOPRQ010000000
VERSION     DAOPRQ010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 12730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12730
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4154_length_12730_cov_13.377199"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1952)
                     /locus_tag="PKJ61_09155"
     CDS             complement(<1..1952)
                     /locus_tag="PKJ61_09155"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HOC14030.1"
                     /translation="MKPNYTRAVRLFGTAVIGAGLALAPLTAAPAQAADEVTINLLNF
                     NDFHGRIATDTTVQFAGTIEKERATYGVDNTLLLSAGDNIGASLFASATQADQPTIDV
                     LNALNVKASAVGNHEFDKGFADLTGRVIGATPNAKWDHLGANVYKVGTQIPALPEYKV
                     YTVGGITVGVIGVVTQETPSLVSSIGIKDITFGDPVEAVNRVAAQLSDTDPANGEAQV
                     LVAEYHEGAARSLPVPLADQVAAGGAFAEIVNKTAASVDVIFTAHTHQAYSYTAAKPG
                     GGDRPVLQAASYSDKLAQVVLKVDDTTGNVSSYAAKLIDRTKDKAADLVAAYPVVKTV
                     DDITKAALAYADKVGSEETGKLTADITTAFSGGSYVNGVYAGGTRDDRSKESTMGTLI
                     ADSLLKTLSNAERGGAQIGIVNPGGMRAELLYKGSSATTGDADGKIVRSEANSVLPFV
                     NNLWTTTLTGAQLRSVLEEQWQPAGSSRPYLQLGLSSNVSYTYNSGAAAGSRIGRIMV
                     NGKLVNATDTFRIGSFSFLLAGGDNFNTFKAGTRTLDSGLVDYEAWIDYLKANSPVKP
                     SFAKHGVGVTSSISAKVGQSYTLSVSSLDLTSLGSPANTTLKVTVDGEVVADNVPVTK
                     GAAQTTFIVKHNGPVVLTANPSGTVV"
     gene            complement(2135..3181)
                     /locus_tag="PKJ61_09160"
     CDS             complement(2135..3181)
                     /locus_tag="PKJ61_09160"
                     /inference="COORDINATES: protein motif:HMM:NF018533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HOC14031.1"
                     /translation="MPEGDTVKRTCDRLHAALAGRVLTAAELRWPTLSTANLVGMTVV
                     GVVPYGKHILIRFDSGWTLHSHLRMEGQWRIEHSGPMPGSGPTSDPPALQRQPGPPQR
                     QPGPPQRQPGPPQRHPGEGRDLPPRSVELRAGAVGGRDLRALLANSEWTALGLRLGEL
                     DLVRTAEEGQLIGRLGPDILADDFALATAVANVAQSGTTIGAALLDQRNLAGIGTLWA
                     SEALFLVRLHPWRPAAEFTSSGLAVVIERARRLMLAALPHAVQSTTGSRRPNENTYVH
                     GRSGRPCRRCGATVRVAMIGPPTRERTMFYCPGCQGGLAPTDGGRSIAPLGSGSRDQR
                     PRGAPDGKPRTSRK"
     gene            complement(3185..7990)
                     /locus_tag="PKJ61_09165"
     CDS             complement(3185..7990)
                     /locus_tag="PKJ61_09165"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HOC14032.1"
                     /translation="MPGNVLERFSAATREWFRSNFAAPTSAQAGAWDAISSGRHTLVV
                     APTGSGKTLSAFLWALDRLATEPAPEPSRRCRVLYISPLKALAVDVERNLRAPLVGIG
                     HAATRLGLERPDIRVAVRSGDTPAEERRKFTKQPPDILITTPESLFLLLTSAAREVLT
                     GVETVIVDEVHAVAGAKRGAHLALSLERLDALLDRPAQRIGLSATVQPVDEVARFLSG
                     GREVARVQPPSEKQWDLQVVVPVPDLAAIGEVTGDLSGPAAGDPQRTSIWPHVEERIV
                     DEIAAHSSTLVFVNSRRLAERLTSRLNEIWLERFEAELPIEPVETTKASQAHAEIPGQ
                     ARDDMDPSSVGRSNVIPANAGISPRAPAQVMAQSAISHGAPPVLARAHHGSVSREQRQ
                     LTEEDLKAGRLPAVVATSSLELGIDMGAVDLVIQVEAPPSVASGLQRVGRAGHQVGAV
                     SRGVLFPKFRGDLVQTAVAVERMRAGEIESLHVPANPLDVLAQQIVAMVAMDDWTVGD
                     LEALIRRSAPFATITRPVLESVLDMLAGRYPSDEFAELRPRLVWDRTTDTLSGRRGAQ
                     RLAVTSGGTIPDRGLFGVFLASDGPGRRVGELDEEMVYESRVGDVITLGTSSWRIEEI
                     THDQVRVTPAPGLPARLPFWKGDSIGRPAELGAGIGAFLREVSGLKPAKARERVRAVG
                     LDDWATDNLIAYLNEQTEAIGRIPNDRQLVVERFRDELGDWRVVVHSPYGARVHAPWA
                     LVVTARLRERFGVDVQAMHADDGLVFRLPDTDWLADSDNPLVEQVGTRASHASRSSSM
                     DELVELLFPDPDEIPGLVNEQIGGSALFAARFRECAGRALLLPKRRPDRRQALWQQRL
                     RASQLLEVASQYPSFPIVLEAVRECVNDVFDVPSLVTLLRGVRSRQISTVAIETRQPS
                     PFASSLLFGYVAQFLYEGDSPLAERRAAALSLDPTLLADLLGTSEGLALSELLDPDAL
                     TRTERELQHLDESRKARDADQLTDLLRSLGPLTTAALQARSRAGPHIVGGWLDDLTQA
                     RRVLALPGERWAVIEDAGRVRDALGVPLPVGVPDALAAPVPDPLGDLLARYARSHTVF
                     TAGEAAQWLGIGVAVARDLLRRLVASGRLVEGDLRPPGWLPVSAQPVAHVETPLGQKV
                     ETPARSADHFDATQFCDARVLRLLRRRSLAALREAVEPVSASDFARFLPFWQGFGELR
                     GIDGVLRAIEQLAGVAVPASALESLILPARVIDYHPSMLDELTASGEVVWQGRGSLAG
                     TDGWVSLQPAEMAHLTLAPPAAVPDELAETVLAALGSGGYFFRPLADQLDIADPELTR
                     VLWDLVWSGHLSNDTLAPLRALLGQGRPAHRSRPSAPGRGRYGRLPARSLVAARASAP
                     QVAGRWSALPSLEVSPTERAVGTAEVLLERYGVVTRGSVAAEEIAGGFGGIYPVLAAA
                     EESGRIRRGYFIEGLGAAQFGTVGAVDRLRASARPLEDTPADPRALVLAAADPANPYG
                     AALPWPARAEGGHLPGRKAGALVVLLDGDLVLYVERGGRTLLSWSDDPGRLNPAAAAL
                     AGAVHRGALGKLTVTKVDGESVLGTESQLAAALASAGFGLTPSGLRLRR"
     gene            8153..8452
                     /locus_tag="PKJ61_09170"
     CDS             8153..8452
                     /locus_tag="PKJ61_09170"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HOC14033.1"
                     /translation="MWLSAATTLTEALIVAEARQGREAAADLAALLAELEAEVVSMDE
                     AQAEVAASAWRRFGKGRHPAALNFGDVFSYSLAAVRGEPLLFKGREFAATDVSQA"
     gene            complement(8453..12016)
                     /locus_tag="PKJ61_09175"
     CDS             complement(8453..12016)
                     /locus_tag="PKJ61_09175"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOC14034.1"
                     /translation="MVRLTKASLTHRTVVMLLSLLTIGLGVFAAGALKQELIPSFDLP
                     RGTVLAVYPGAAPEVVEAQVSKPIESAVKGVAGVTNVTSKSSSGVSYITVAWDYGLDP
                     DDMASSIRSAIESVSSSLPTSVDPKVITGSFDDIPIVVLALSSDDDLNTLSGKVTDTV
                     VPALKTIPGVRDVTAAGEETHEIIVTYDQAAIEEYGIDPTTIGQFFAANANAIPSGTL
                     RTDATNLDVQTGTTYASVKDIENLLLQGTDGPVKLSDIATVKEQPVETTSISRVNGRP
                     ALTLSVTKEAGANTVSVANGVAEALPELEQQLGGGAEFATVFDQAPYIEQSIHDLSIE
                     GGIGLVMAVLVIMVFLGSIRPTLITAISIPLSLLIALIGLWVGGYSLNILTLGALTVA
                     IGRVVDDSIVVIENIKRHQGYGEFGRTSIVNAVREVAGAVTSSTLTTVAVFAPIGLVG
                     GQAGQFFRPFAMTVTIALVASLFVSLTVVPVLASWFMRPTAKQQAAQTEAAEEEEKDT
                     WLQKSYLPVLNWTLAHRWITLAIALGLFAGTLALAPLLKTDFIGSMGNESLTITQKLP
                     SGTGLAETDAAAAKVEQLLAQNPSVDAYSTSIGGSTSVFMGSQADTNQATFTVPLKAG
                     GDGKVVATELRRQLADLGSSVGEVEVSVGSGQGGATGVVLYVESSDSEQLMVANDQVL
                     EMMKTVPGLTNIASDLSEARDQLSVDVDEAKAADKGMTQASIGQAVARSVRGQLIGTL
                     AQGDTTLNVYLRSQAPVKNIKELREIKLPVTQLMNAQAKSDAADKVSEDSEKLQADSK
                     KTQTTAYNDQVKALEKSRSAAKKSAAKLEKQLAKAKKQLKSLQSQLNQIEQLPPDQQG
                     PTVSAAVFKLTQAVSGVSMQVVQLAQGVAASKAQVTAVNKQLEGLAESRTKTLDAQDK
                     QQALSDAGKEAGKATAKPIKLKAVADVQLVNAPAMITRVDGTRAATLTASSESSDLGT
                     TTSQIKAGIAALDLPDGVTVRVGGVSQQQTESFQQLGLAMLIAIFAVYLIMVATFGSL
                     LQPLVLLVSIPFAATGALGLSLLTDTALGVPSMIGLLMLIGIVVTNAIVLIDLINQKR
                     KAGLGVDASIQAGARLRVRPIIMTALATIFALIPMSLGLTGGGVFISKPLAIVVIGGL
                     ISSTLLTLILVPVLYDLLETWREKRANRRAARKAEPVVVAD"
     gene            12108..12596
                     /locus_tag="PKJ61_09180"
     CDS             12108..12596
                     /locus_tag="PKJ61_09180"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOC14035.1"
                     /translation="MHQLGGLALDEERAAAARELWELQAQVHDRAVGMVGPMPLPPDL
                     TMQQVRVLGCVVKEPGLSGHELGKRIGVSAPTASGLVERLVEKGLIARTDDPDDRRIR
                     RLHPTTDGLAVIREMDSMFSRSLGALIEHLSMEDLDLLRRGTLAMLSALDRAIASANA
                     SG"
BASE COUNT         1956 a         4255 c         4445 g         2074 t
ORIGIN      
        1 tgcaccacgg tgccggacgg gttcgcggtc agcacgacgg gaccgttgtg cttcacgatg
       61 aaggtcgtct gcgcagcgcc cttcgtgacg ggcacattgt ccgcgaccac ttcgccgtcc
      121 acggtcacct tgagcgtggt gttggccggc gaaccgaggg aggtcaggtc gaggctggat
      181 accgagagcg tgtagctctg gcccaccttg gcggaaatgg acgaagtgac gcccacgccg
      241 tgcttcgcga agctcggctt caccggcgag ttggccttca ggtagtcgat ccacgcctcg
      301 tagtcgacca ggccggagtc caaggtgcgc gtgccggcct tgaaggtgtt gaagttgtcg
      361 ccaccggcca gcaggaagct gaacgaaccg atccggaaag tgtcggtcgc gttgaccagc
      421 ttgccgttga ccatgatccg gccgatccgg ctaccagcgg ccgcgcctga gttgtaggtg
      481 tagctcacgt tgctggacaa cccgagctgc aggtacggac ggctggagcc agccggctgc
      541 cactgctcct ccaggaccga cctgagctgt gcgccggtga gcgtagtggt ccacaggttg
      601 ttcacgaacg gcagcaccga gttggcctcg gagcggacga tcttgccgtc ggcgtcgccg
      661 gtggtggcgg agctgccctt gtaaagcagc tcggcacgca tgccgcccgg gttcacgatg
      721 ccgatctgag cgccgccgcg ttccgcgttc gacaacgtct tcagcaggga atcggcgatc
      781 agggtgccca tcgtggactc cttggaacgg tcgtcccgcg tcccgccggc gtagacgccg
      841 ttgacgtagc tgccgccgct gaaagcggtg gtgatgtctg cggtcagctt gccggtctcc
      901 tcggagccga ccttgtcggc gtaggcgagc gctgccttgg tgatgtcgtc gaccgtcttg
      961 accaccgggt atgcggccac cagatcggcg gccttgtcct tggtgcggtc gatcagcttg
     1021 gcggcgtagc tggatacgtt gccggtggtg tcgtccacct tgagtacgac ctgggccagc
     1081 ttgtcgctgt aggacgcggc ctgcagcacc ggacgatcgc caccgccggg cttggcagcg
     1141 gtgtagctgt acgcctggtg ggtgtgcgcg gtgaagatga cgtcgaccga agccgcggtc
     1201 ttgttcacga tctcggcgaa cgcgccgccg gcggcgacct ggtcggccag cggcaccggg
     1261 aggctccggg cggcaccttc gtggtactcg gccacgagca cctgggcttc gccgttggcc
     1321 gggtcggtgt cggagagctg ggcagcgacc cggttcacgg cctcgaccgg atcaccgaac
     1381 gtgatgtcct tgatgccgat ggacgagacc agcgaggggg tctcctgggt gaccacgccg
     1441 atcacgccga cggtgatacc gccgacggtg tagaccttgt actcgggcag tgccgggatc
     1501 tgggtgccga ccttgtagac gttggcgccg aggtggtccc acttggcgtt cggagtggcg
     1561 ccgatcaccc gtccggtgag gtcggcgaag cccttgtcga actcgtggtt gccgaccgcg
     1621 gacgccttga cattgagtgc gttcagcacg tcgatggtgg gctggtcggc ctgcgtggcg
     1681 gacgcgaaca gcgaggcgcc gatgttgtcg ccggccgaga gcagcagcgt gttgtcgacg
     1741 ccataggtcg cgcgctcctt ctcgatcgtg ccggcgaact gaacggtggt gtcggtcgcg
     1801 atccggccgt ggaagtcatt gaagttcagc aggttgatcg tcacttcgtc cgctgcctgc
     1861 gctggtgctg cagtgagtgg tgcgagtgcc aggccggcgc cgatgacggc tgttccgaac
     1921 aagcggacgg ccctggtgta atttggtttc acccgttctc cttaaggtgc tgacaagggt
     1981 agttaccgga ttcggatcgg gcggtcctgg ccggatgtgg cccgggcggc cctgcccgag
     2041 acttccggcc aactctgatt cggggctggc cggatcggtc catcatggtc tccggacagg
     2101 tcacctgtcg atggaatcga ggaactcgcg gtacctattt gcgcgaagtg cgcggctttc
     2161 cgtctggggc gccgcggggc cgctggtcac ggctcccgga accgagcggt gcgatcgaac
     2221 ggccgccgtc ggttggggcg aggccaccct ggcagccggg gcagtagaac attgtccgct
     2281 cccgggtcgg cggcccgatc attgctaccc gcacggtcgc cccgcagcgg cgacagggcc
     2341 tccccgaacg accgtgtaca taagtgttct cgttcggccg gcggctgccc gtggtcgatt
     2401 gcaccgcatg cggtagcgcg gccagcatca gccggcgggc acgctcgatg acgaccgcca
     2461 gtccgctcga ggtgaactct gcagccggcc tccacgggtg caggcgcacc aggaagaggg
     2521 cctcgctggc ccacagggtg ccgataccgg cgaggttgcg ctggtcgagc agggcagcgc
     2581 cgatagtcgt gccgctctgg gcgacgttgg ccacagcagt agcgagggcg aagtcgtcgg
     2641 cgaggatgtc cggaccgagc cggccgatca gttggccttc ctcggccgtc cgcaccagat
     2701 cgagttcgcc cagccgcagc cccagggccg tccactcgga gttggccagc agggcgcgaa
     2761 ggtcgcgtcc gccaaccgca cctgcgcgca gttcgactga tcgcgggggg agatcccggc
     2821 cttcgccggg atgacgttgg ggtgggccgg gctgacgttg gggtgggccg ggctgacgtt
     2881 ggggtgggcc gggctgacgc tggagtgcgg gtgggtctga cgttgggccc gagccaggca
     2941 ttgggccgga gtgctcgatt cgccactggc cctccatacg caggtgggaa tgcagcgtcc
     3001 aaccggaatc gaaccggatc aggatgtgct ttccgtacgg caccaccccg accacggtca
     3061 tgccgaccag gttcgccgtg gaaagggtcg gccagcgcaa ctcggccgcg gtcagcacgc
     3121 gtccggcgag tgcggcgtgc agccggtcac aggtgcgctt gactgtgtcg ccttcaggca
     3181 tcgtttaccg ccgcagcctc agcccgctcg gcgtgagtcc gaatccggcg gacgctaggg
     3241 cggccgccag ctgcgactcg gtgcctagca cggattcgcc gtcgaccttc gtcacggtca
     3301 gcttgcccag tgcaccgcga tggacggcgc cggcgagcgc cgctgcggcc gggttgagcc
     3361 ggcccggatc gtccgaccag gagagcagcg tgcgcccgcc gcgttccacg tagagcacga
     3421 gatcgccgtc taatagcacc accagcgcgc cggccttgcg tccgggcagg tggccgccct
     3481 cggctcgggc tggccagggg agtgccgcgc cataggggtt ggctgggtcg gccgcggcca
     3541 gcaccagcgc gcgggggtcg gctggcgtgt cctcaagcgg acgggcggac gcgcgcagcc
     3601 ggtcgacggc gccgaccgtg ccgaattggg ctgcgccgag cccttcgatg aagtagccgc
     3661 gacggatccg gccggactcc tcggctgcgg ccagcaccgg atagatgccg ccgaaaccac
     3721 cggcgatctc ctctgcggcg acgctgcccc tggtcaccac gccgtagcgc tccagcagca
     3781 cctcggcggt gcccacggcc cgctctgttg ggctgacctc caggctcggc aacgcggacc
     3841 agcgtccggc cacctgagga gcggacgcac gggcggcgac cagcgatctc gcgggcaggc
     3901 ggccgtaacg cccgcgaccg ggagcggacg ggcggctgcg atgcgccggt cggccctgac
     3961 ccagcaacgc ccgcagtggg gcaagagtgt cgttgctgag gtggcccgac cagaccaggt
     4021 cccaaagcac gcgggtgagc tcggggtccg cgatgtcgag ttggtcggcg agcggacgga
     4081 agaagtaacc accgctgccg agcgccgcga ggaccgtttc ggccagttcg tccggtaccg
     4141 cggccggcgg cgcgagggtg aggtgggcca tctcggcggg ttgcagcgag acccagccgt
     4201 ccgtgcccgc gaggctgccg cggccctgcc agaccacctc gccgctggcg gtgagctcgt
     4261 cgagcatgct ggggtggtag tcgatcaccc gggcaggcag gatcagcgac tccagcgcgc
     4321 tcgccggcac cgccaccccg gccagttgct cgatcgcacg caggaccccg tcgataccac
     4381 gcaactcgcc gaagccctgc cagaaaggca ggaaacgcgc gaagtcactc gcgctgaccg
     4441 gttccaccgc ctcgcgcagc gccgccaggg accgccggcg cagcagcctg agcacacgcg
     4501 cgtcacagaa ctgggtggcg tcgaagtgat cggcgctgcg ggccggggtc tcgactttct
     4561 ggcccagcgg ggtctcgaca tgcgcgaccg gctgagccga gactggcaac cagccgggcg
     4621 gacgcaggtc gccctcgacc aggcgtccgg acgcaaccag gcggcgcagc agatctcggg
     4681 ccacggcgac gccgataccg agccactggg cggcctcgcc agcggtgaac accgtgtggc
     4741 tacgggcgta gcgtgcgagc aggtcaccga gcgggtcggg cactggggcg gccagcgcgt
     4801 ccggcacgcc gaccggcagg ggcacgccga gggcgtcgcg aacccttccg gcgtcctcga
     4861 tcaccgccca gcgctcgccc ggtagcgcca gcacccgccg tgcctgggtg aggtcgtcca
     4921 gccacccgcc cacgatgtgc ggcccagccc gagaccgcgc ctgcaaagcc gccgtggtca
     4981 gcgggccgag gctccgcaac aggtcggtga gttggtcggc gtccctggct ttcctggact
     5041 cgtccaggtg ctgcaactcc cgttcggtcc gggtcagggc gtccgggtcg agcaactccg
     5101 aaagcgccag cccctcgctg gtgccgagca gatcggccag cagcgtgggg tcgaggctga
     5161 gcgcggccgc gcggcgctca gctaggggtg agtcgccctc gtagaggaac tgcgcgacgt
     5221 agccgaacag cagggaggag gcgaacggcg acggctgtcg ggtctcgatc gccacggtag
     5281 aaatctggcg ggagcggacg ccgcgcagca gcgttaccag gctgggcacg tcgaagacgt
     5341 cgttcacgca ctcgcggacg gcctccagca cgatcgggaa gctcggatac tggctggcca
     5401 cctccaacaa ttgggacgcg cggagccgtt gctgccacag cgcctgccgc cggtccggac
     5461 gccgcttggg cagcagcagg gcccgtccgg cgcactcccg gaagcgggcg gcgaacagtg
     5521 cggagccgcc gatctgctcg ttgaccaggc ccgggatctc gtctggatcc gggaagagca
     5581 gctcgaccag ctcatccatg gacgaactgc gggatgcatg gcttgctcgg gtcccgacct
     5641 gctcgaccag tggattgtcc gaatcggcca gccagtcggt gtcgggcagc cggaagacca
     5701 ggccgtcgtc ggcgtgcatg gcctgcacgt ccacgccgaa gcgctcccgc agccgcgcgg
     5761 tcaccaccag cgcccacggg gcgtgcacgc gcgcgccgta gggcgagtgc acgaccaccc
     5821 gccagtcgcc caactcgtcg cggaagcgtt ccaccacgag ctgacggtcg ttggggatgc
     5881 gtccgatcgc ctcggtctgc tcgttcaggt aggcaatcag gttgtcggtc gcccagtcgt
     5941 cgagcccgac ggcgcggacg cgttcgcggg ccttggccgg tttgagcccg gacacctcac
     6001 gcaggaaggc gccgatccct gccccgagtt cggctggccg cccgatcgaa tcgcccttcc
     6061 agaagggaag acgagcgggc aggccggggg ccggggtgac ccgaacctgg tcatgggtga
     6121 tctcctcgat ccgccacgac gaggtgccga gggtgatcac gtcacccacc cgggactcgt
     6181 agaccatctc ctcgtcgagc tcgccgacgc gccggccagg accgtcggag gcgaggaaga
     6241 ccccgaacag gccgcggtcg gggatggtgc cgccgctggt caccgcgagc cgttgcgcgc
     6301 cgcggcgtcc ggagagggtg tcggtggtgc ggtcccagac caggcgcgga cgcagctccg
     6361 cgaattcgtc ggacgggtag cgtccggcca gcatgtcgag caccgattcg agcaccgggc
     6421 gggtgatcgt ggcgaagggc gccgatcttc ggatcagggc ctccaggtcc cccaccgtcc
     6481 agtcgtccat cgccaccatc gcgacgatct gctgcgcgag cacgtctaag gggttggccg
     6541 ggacgtgcag cgactcgatc tcgccggccc gcatgcgctc caccgcgacc gctgtctgca
     6601 ccaggtcgcc gcggaacttg gggaacaata cgccccgcga cacggcgccc acctggtgcc
     6661 cggcccggcc cacgcgctgc agcccggacg cgaccgaagg cggcgcctca acctggatga
     6721 ccagatcgac cgcacccatg tcgatgccca gttcgaggga cgatgtcgcc accaccgccg
     6781 gcaggcgtcc ggccttcaga tcttcctcgg tgagctgccg ctgctcccgg ctgaccgagc
     6841 cgtggtgcgc cctggccagt accggtggcg cgccgtggct gatcgccgac tgcgccatca
     6901 cctgagcggg cgcgcgcggc gagatcccgg cgttcgccgg gatgacgtta ctgcggccca
     6961 cgctgctggg atccatgtca tcccgggctt gacccgggat ctccgcgtgg gcctgggagg
     7021 ctttggtggt ctcgaccggc tcgatcggca actcggcctc gaagcgctcc aaccagatct
     7081 cgttgagccg cgacgtcaag cgctcagcga gccgacggga gttgacgaag accaaggtgg
     7141 aggagtgtgc agcgatttcg tcgacgatcc gctcctcgac gtgcggccag atcgaggtgc
     7201 gctgcggatc acccgctgcc ggcccggaga ggtcaccggt cacctcgccg atcgcggcca
     7261 agtcaggcac cggcaccacc acctgcaggt cccactgttt ctcgctcggt ggctgcacgc
     7321 gggcgacctc gcgtccgccg gacaggaagc gagccacctc atcgaccggc tgaaccgtgg
     7381 ctgacaagcc gattcgctgt gcgggccggt ccagcagggc gtccaggcgc tccaggctca
     7441 gcgccaggtg ggcgcctcgt ttcgcgccgg cgacggcgtg cacctcgtcc acgatcacgg
     7501 tctcgacgcc ggtcaacacc tcgcgagctg cggatgtgag cagcaggaac agcgactcgg
     7561 gcgtggtgat caggatgtcc ggtggctgtt tggtgaactt gcgccgctcc tcggccgggg
     7621 tgtcgcccga gcgcaccgcg acccggatgt cgggacgctc aagtccgagc cgggtggccg
     7681 catgtccgat gccgaccagg ggagcgcgca ggttgcgttc gacgtcgaca gcaagcgcct
     7741 tcagcgggga gatgtagagc acccgacagc gcctgctcgg ctcaggcgcg ggctcagtgg
     7801 ccagccggtc aagtgcccaa aggaaggccg acagcgtctt gccggagcca gtcggcgcga
     7861 cgaccagcgt gtggcgacct gacgagatcg cgtcccaggc gccggcctgc gccgaggtgg
     7921 gcgcggcgaa gttgctccgg aaccactccc tggtggctgc ggagaaccgt tccaacacgt
     7981 ttcccggcat ggtgcatcaa ctgtgccacg acccactgac agcactcggc cgcgcacccc
     8041 tacccgacga cggacgcact ggcgcgagaa ctcgctgccc agatcgggga gagcatcacc
     8101 gaggcgatcc ggctggcgat gagcgagcga ctcgcccggt tgcggcgcca gaatctggct
     8161 ttcagcagcc accaccctca ctgaggcact gatcgtcgcc gaggcccgac aggggagaga
     8221 ggcagccgcc gatctggccg ccttgctcgc cgaacttgaa gcggaggtgg tcagcatgga
     8281 cgaagctcag gccgaggtcg ccgcgtccgc ttggcggagg tttggcaagg gccggcatcc
     8341 ggccgcgctg aacttcggcg acgtcttcag ctactcgctg gccgcggtcc gcggcgaacc
     8401 actgctcttc aagggccggg agttcgccgc caccgacgtc agccaagcgt gatcagtccg
     8461 ccacgaccac cggttcggcc ttgcgtgcag cccgccgatt ggcgcgcttc tcccgccaag
     8521 tctccagtag gtcatagagc accggcacca ggatcagggt gagcagagtg gacgagatca
     8581 gcccgccgat caccacgatt gccagcggct tggagatgaa caccccgccg ccggtcagcc
     8641 cgaggctcat cggaatcagg gcgaagatcg tcgccagcgc ggtcatgatg atcggccgca
     8701 cccgcagccg cgcgcccgcc tgaatggacg cgtccacgcc caacccggcc ttgcgtttct
     8761 ggttgatcag gtcgatcagc acgatcgcat tggtcaccac gatgccgatg agcatcagca
     8821 ggccgatcat gctgggcacg ccgagcgcag tgtcggtgag cagcgacagg cccagtgcac
     8881 ccgtcgccgc gaacggaatc gacaccagca ggaccaacgg ctgcagcagc gagccgaacg
     8941 tcgccaccat gatcaggtac accgcgaaga tggcgatcaa catcgccagc ccgagctgct
     9001 ggaacgactc ggtctgctgc tgcgacaccc cgccgacgcg gacggtgacc ccgtccggta
     9061 ggtcgagcgc cgcgatgcct gctttgatct ggctggttgt ggtgccgagg tcgctcgact
     9121 ccgaggaggc ggtcaacgtg gccgcgcgcg tcccgtccac ccgggtgatc atcgccggcg
     9181 cgttgaccag ctggacgtca gcgacagcct tcagcttgat cggcttggcg gtcgccttcc
     9241 ccgcctcctt gccggcgtcg ctgagcgcct gctgcttgtc ctgtgcatcg agggtcttgg
     9301 tgcgactctc cgccagcccc tccagttgct tgttcacagc cgtcacttgg gccttggacg
     9361 cggcgacgcc ctgcgccaac tgcaccacct gcatgctcac gcccgagacc gcctgggtga
     9421 gcttgaacac cgcagcgctg accgtcgggc cctgctggtc gggcggcaac tgttcgatct
     9481 ggttgagctg ggactgcagc gacttcaact gcttcttcgc cttcgccagc tgcttctcca
     9541 gcttcgccgc cgacttcttg gccgccgagc ggctcttctc cagcgccttc acctggtcgt
     9601 tataggcggt ggtctgggtc ttcttggagt cagcttggag cttctccgaa tcctcactga
     9661 ccttgtccgc agcatcgctc ttggcttgtg cgttcatgag ctgcgtcacc ggcagcttga
     9721 tctcgcgcag ctccttgatg ttcttcaccg gcgcctgcga gcgcaagtag acgttcaggg
     9781 tggtgtcgcc ctgggccaac gtcccgatca gctgcccccg caccgacctg gcgaccgcct
     9841 ggccgatcga cgcctgggtc atgcccttgt cagcggcctt cgcttcgtcc acatcgaccg
     9901 aaagctggtc ccgggcctcg ctcaggtcgc tggcgatgtt ggtcaatcct gggacggtct
     9961 tcatcatttc gagcacctgg tcgttggcga ccatgagctg ttcggagtct gagctctcca
    10021 cgtacagcac caccccggtg gcgccgccct gaccgctacc caccgacacc tcgacctcac
    10081 ccacggacga gccgaggtca gccagttggc ggcgcagttc ggtggcaacc accttgccgt
    10141 caccaccggc cttcaatggc acggtgaagg tggcttggtt ggtgtcggcc tggctgccca
    10201 taaacaccga ggtggagccg ccgatgctcg tggagtatgc gtccacggac gggttctgcg
    10261 cgagcagctg ctccaccttg gccgcggccg cgtccgtctc ggccagaccc gtgcccgaag
    10321 gcagcttctg ggtgatggtc aggctctcgt tgcccatgct gccgatgaag tcggtcttca
    10381 gcaggggtgc cagtgccagc gtcccggcga acaggccgag cgcgatcgcg agggtgatcc
    10441 agcggtgggc cagcgtccag ttcagcaccg gaaggtagct cttctgcagc caggtgtcct
    10501 tctcttcttc ctctgcagct tcggtctggg cggcctgctg cttcgcggtc ggacgcatga
    10561 accaggacgc cagcaccggc acgaccgtca gcgagacgaa caacgacgcg accagcgcga
    10621 tcgtcacggt catcgcgaag ggacggaaga actggccggc ctgaccgccg acgagcccga
    10681 tcggggcgaa caccgcgacg gtggtcaggg tggatgaagt gaccgcgccg gccacctcgc
    10741 ggacggcgtt cacgatcgac gtgcgtccga actcgccgta gccctgatgg cgtttgatgt
    10801 tctcgatgac gacgatcgag tcgtcgacca cgcgtccgat cgcgacggtg agcgcgccga
    10861 gggtgaggat gttcagggag tagccgccta cccaaagtcc gatcagggcg atcaggagcg
    10921 acaacgggat cgagatcgcc gtgatcagcg tcggccggat cgagcccagg aacaccatga
    10981 tcaccagcac ggccatgacc aggccgattc cgccttcgat gctcaggtca tggatcgact
    11041 gctcgatgta gggggcctgg tcgaagacgg ttgcgaactc agcaccgccg ccgagttgct
    11101 gctcgagttc ggggagcgct tcggcgaccc cgttggccac gctgaccgtg tttgcgcccg
    11161 cttccttggt gaccgaaagg gtgagggcgg ggcggccgtt gacccgggag atgctggtcg
    11221 tctctaccgg ctgttccttg actgtggcga tatcgctgag cttcaccggg ccgtccgtgc
    11281 cctgcagcag gaggttctcg atgtccttga ccgaggcgta ggtggtgccc gtctgtacgt
    11341 ccaggttggt cgcatcggta cgcagcgttc cggacgggat cgcgttggca ttggcggcga
    11401 agaactgccc gatcgtggtc gggtcgatgc cgtactcctc gatcgccgcc tggtcgtagg
    11461 tgacgatgat ctcgtgagtc tcctcaccgg cggcggttac gtcccggacg cccgggatgg
    11521 tcttgagcgc ggggacgaca gtgtcggtga ccttgccgga cagcgtgttc aggtcgtcgt
    11581 ccgacgagag ggcgaggacc acgatcggga tgtcgtcgaa ggaaccggtg atcaccttcg
    11641 gatcgaccga ggtcggcagg ctggaggaga ccgactcgat cgcgctgcgg atgctgctgg
    11701 ccatgtcgtc gggatcgaga ccgtagtccc aggcgacggt gatgtaggag acgccgctgg
    11761 agctcttgga cgtgacgttg gtcacgccgg ccacgccctt gaccgccgac tcgatcggtt
    11821 tggagacctg cgcctcgacc acttccgggg ccgcgccggg gtagaccgcc aggacggtgc
    11881 cgcgcggcag gtcgaacgac gggatcagct cctgcttcag ggcaccggca gcgaagacgc
    11941 cgagcccgat cgtgagcagg ctgagcagca tcacgacagt gcgatgcgta aggctggcct
    12001 tggtcagacg taccactaag gtccccttca cggtcctgaa ggcgccggtc agccgacgca
    12061 tccgttagca tagctctaac tatttctatg gccgaaaggg ttcgacagtg caccagctgg
    12121 gaggcctcgc gcttgacgag gagcgtgcgg ctgcggcccg cgagctctgg gagttgcagg
    12181 cccaggtgca cgatcgcgcg gtaggcatgg tcggcccgat gccgctgccg cctgacctga
    12241 ccatgcagca ggtgcgcgtc ctcggctgcg tggtcaagga gcccgggctt tcaggccacg
    12301 agctcggcaa gcggatcggg gtcagcgcgc ccaccgccag cggcctggtg gagcgcctgg
    12361 tggagaaggg gttgatcgcg cgtacggacg acccggacga ccggcggatc cgccggctgc
    12421 acccgaccac cgacgggctg gccgtgattc gtgagatgga ctcgatgttc tcccgctccc
    12481 ttggtgcgct catcgagcac ctgtcgatgg aggacctgga cctgctgcgc cgaggcaccc
    12541 tggccatgct ctccgcactg gaccgggcca tcgcttccgc gaacgcgtcc ggctagttcg
    12601 ggttccaggg gccggccgag cgtccggatt cgagctttcc gtgcggatgc gcactcggcc
    12661 gtcggcgccc tcttcgtccg ttttgtctcg gtggtaacgc gacacgccga cgacacgccg
    12721 tggggtctgg
//
LOCUS       DAOPRQ010000067        12157 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_4415_length_12157_cov_11.667080, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000067 DAOPRQ010000000
VERSION     DAOPRQ010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 12157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12157
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4415_length_12157_cov_11.667080"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(151..951)
                     /locus_tag="PKJ61_09185"
     CDS             complement(151..951)
                     /locus_tag="PKJ61_09185"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOC14036.1"
                     /translation="MDARAVALLACPVCAQHLELSPGVLRCPAGHSYDLARQGHVNLL
                     GGAEPANADTPAMLQARGRVHASGLFDEVAEAVAAQVRGSRLLEAGAGNGFYLARALE
                     RRPEAVGIALDVSKAAARAAVRAHPRIGAVVADVWRNLPIRDRCLDTVLCVFAPRNLP
                     EFRRVLEADGRLVVVLPNAGHLDGLRDRYGLLKVDADKAEQLTEAAAGLFEPVESVRI
                     SAASQVDAERALDLIAMGPNAFHRLPEVASEGEITVDVTLLSFRPLPT"
     gene            complement(966..1751)
                     /locus_tag="PKJ61_09190"
     CDS             complement(966..1751)
                     /locus_tag="PKJ61_09190"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HOC14037.1"
                     /translation="MRRYAIWTVGLVALLSACSTTTPAAPAPASSSVASAPALSGTSW
                     TVTSIKGTATLDGHQPTMLFEGSTVSGLASCNRFTGGFTQDGANLKFDTFASTAMMCT
                     DAAVMTQEQTFLGTVPTVTNLRSVDSGLELLDAGAQVLVVLAPVVDKPLAGTAWKLSG
                     IVTNDAVTAPVEGSSVTMTIQDGQLSGKACNNFSGTVETSDDGSFKAGPLRSTKMACA
                     NEALSAEETAVLATLQKATSFVIEGSSLTLKAGDGTGLEFLAS"
     gene            complement(1754..2548)
                     /locus_tag="PKJ61_09195"
     CDS             complement(1754..2548)
                     /locus_tag="PKJ61_09195"
                     /inference="COORDINATES: protein motif:HMM:NF014193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HOC14038.1"
                     /translation="MKLLNTPMGVAPSGRRPWLRWVALAVFVVALAIAFVNLGRWQLD
                     RLDQRRERNSAVVEHESGPLREFADVFNRPITEADQWQRVRVRGTYDAANQFLVRYRV
                     QGADSGYEIVTPLQATSGAWVLVDRGFAVKPPDQDYPKSLPAPPSGQVEIVGYVRRDE
                     TGSTDAMTPTQQTIRLVNSTALAQALGRELVNGYLSAIETRPGDPSGLVPVQPPELNE
                     GNHFSYALQWFMFAGLAGVGFFLLIRSDVRAMRRSSKEPAAGVEEG"
     gene            complement(2545..2868)
                     /locus_tag="PKJ61_09200"
     CDS             complement(2545..2868)
                     /locus_tag="PKJ61_09200"
                     /inference="COORDINATES: protein motif:HMM:NF022742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3099 domain-containing protein"
                     /protein_id="HOC14039.1"
                     /translation="MSPGNDSKPALITSARTSRSLDTDARRFRYLWTMAVRVACFIAA
                     TVTPLPWNLLLLVAAAVLPAIAVMLANAIDQRTPPAPTAPAPSDRPSLPSPGVVDGTV
                     EDETE"
     gene            2976..3695
                     /gene="fabG"
                     /locus_tag="PKJ61_09205"
     CDS             2976..3695
                     /gene="fabG"
                     /locus_tag="PKJ61_09205"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002523230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HOC14040.1"
                     /translation="MSTNPIARSVVVTGGNRGIGRAIVERFLAAGHKVASLDLGGEAP
                     AGAIGINCNVTDAEQVDAAFAAAEEANGPVEILVANAGITKDTLILRMSDADWDAVLN
                     VNLSGAFRCARRAAKSMIRQRFGRIVFTGSVVALYGSAGQVNYSASKSALVGMARSLT
                     REIGSRGVTANVVAPGFIETDMTAVLSEDVIARYLASIPAARLGQPEEVAAAVAFLAG
                     EEAGFISGAVLPVDGGLGMGH"
     gene            3706..4476
                     /gene="fabI"
                     /locus_tag="PKJ61_09210"
     CDS             3706..4476
                     /gene="fabI"
                     /locus_tag="PKJ61_09210"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004808724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HOC14041.1"
                     /translation="MGILEGKNILVAGVTMNTSIGYRVAEIAQAEGANVVVSNFGRAM
                     SLTARVLNRLDSPPPLLELDVTDESHLASLADRLGEHFDRLDGVVHSIAFANPETALG
                     GKFLSTPFSDVGFSVNVSAYSLVSLAIACKPLFAPSAAIVGLTFDARTSWPIYDWMGV
                     SKAALESTSRYLARYLGPEGVRCNLVAAGPLETIAKKAIPGSEEFNNIWGKRAPLGWD
                     PKDLTPTAKAVVALLSDWFPATTGEMLHVDGGLHSTGA"
     gene            4497..5312
                     /locus_tag="PKJ61_09215"
     CDS             4497..5312
                     /locus_tag="PKJ61_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016455784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOC14042.1"
                     /translation="MNVVQLEGVSIIRSGSTLLDNVSWTVQEDERWVVIGPNGAGKTT
                     MLQVLGAQWHPSRGSASILGERLGAVDVFELRPRIGVTSAALADRIPKSERVSDVVVS
                     AAYAVMGRWREDYDELDYERARQLMQQLRVDHLADRTFGTLSEGERKRVQIARALMTD
                     PELLLLDEPAAGLDLAGREALVTTLSELCTHELSPATVLVTHHVEEIPLGVTHALLLK
                     HGRVVAAGPIEKTLTGPLLSATFDLLLEVSNVGGRWAARAAARAGHHLPTRLA"
     gene            5355..5813
                     /locus_tag="PKJ61_09220"
     CDS             5355..5813
                     /locus_tag="PKJ61_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOC14043.1"
                     /translation="MAEWLAEHAWAAWMVLAALLAMAEMLTLDFTLLMLAVGALAGGL
                     VALAFPGLIWVQFLVAVVVAVAMLGLLRPTLLRKLRDLPGYRSSIDKMVGSSGVALTE
                     ITASAGEVKVAGEVWTAHSLEGTIAAGTEIEIYRVDGATVIVYPRHLALP"
     gene            5831..7009
                     /locus_tag="PKJ61_09225"
     CDS             5831..7009
                     /locus_tag="PKJ61_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004808720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOC14044.1"
                     /translation="MEYVIIVLWLLVAAVVVTVLLRSVRQIRQQQVAVVERLGKFKRT
                     LDPGLHILVPFVDSIRYVMDMREAVVPFPPQGVITEDNLMVNIDSVIYYQIVDPVRAA
                     YEAQNYIQAIEQLTMTTLRNIIGGLDLEQTLTSREEINQKLRTVLDEATGKWGIKVNR
                     VELRSIEPPATIRDAMEKGARAERDKRAAILTAEGQRQSQILSASGDREASILRAQGD
                     REAQVLRAQADRQASMLRAEGEAQAITTTFAAIHAGKPSQSLLAYQYMRMLPNLARGE
                     SNKVWIIPSELNDALKGLGQVAGNALGARVGAEPEAAEGEFEAPARVDVFAEIEEQKT
                     KDEADSAKSVQQAIAEAEALEGRKAARLAVENQNALTRGVPAAEPVQQAEPEAEPEPA
                     "
     gene            complement(7013..7984)
                     /locus_tag="PKJ61_09230"
     CDS             complement(7013..7984)
                     /locus_tag="PKJ61_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016665757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HOC14045.1"
                     /translation="MSWLSRIRIDRFLLAIILAAVVASILPASGFWADALDWFTAAAI
                     ALLFFLYGARLSPAETLAGLRHWRLHTTILSFTYVVFPLLGLALVALTPGLLSPGLQA
                     GLLYLTLVPSTVQSSIAFTSIARGNVAGAVVSASASNILGVFLTPLLCWLLLSNTGQI
                     SNTASTIVEIVLQIFLPFVLGQLSRRWTAGFVSAHPELKLVDRGSIILVVYSAFSAGM
                     REGMWAQTSVVELSRLVVLSLLILAGMLALTWWAARRLGFSRADAIAIQFCGTKKSLA
                     SGLPMAGVLFAGQPLGLLMLPLVIFHQAQLMACSALAGRYAKVAPGD"
     gene            8017..8820
                     /locus_tag="PKJ61_09235"
     CDS             8017..8820
                     /locus_tag="PKJ61_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002520242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOC14046.1"
                     /translation="MATLIHLDRADDERLGDYVGLREATLRRLLETERGVFIAEGSKV
                     IRRAIEAGYPPRSFLLAERWLADLADLLDGLDVPVYVTTEEVAEAVTGFHVHRGALAS
                     LYRAERFTIADVLAGRRVMVLQDLVDHTNVGAVIRNAAGLGWDGVLVSARCADPLYRR
                     SIKVSMGTVFALPWARLPEYVDLAGVLHSAGFTVAALALRPDAVELADFAAGLAKEAR
                     VAVLLGTEGHGLSAGWTDAADAVVRIPMKSGVDSLNVAAASAIAGYALG"
     gene            complement(8962..9552)
                     /gene="def"
                     /locus_tag="PKJ61_09240"
     CDS             complement(8962..9552)
                     /gene="def"
                     /locus_tag="PKJ61_09240"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOC14047.1"
                     /translation="MGIEELTTGGKLRRVTRWSEPVLHAQTRAVTAFDDELRELVRDM
                     FATMAASHGVGLAATQVGVDLAVFIYDCPDADEIHQIGAVCNPVVTTPEGDQRNLVSE
                     DEGCLSLPGGYQPLARPDKAICTGVDPWGNPVTIEGTGMLARCLQHETDHLNGTVFGD
                     RLSGRLRRKLYAQHEELAHLYPDDWPLTPKRTSADG"
     gene            9639..10595
                     /locus_tag="PKJ61_09245"
     CDS             9639..10595
                     /locus_tag="PKJ61_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008363307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HOC14048.1"
                     /translation="MTASEDFLTPAAARMVTEFAGHLRLERGLSPHTVRAYTSDVTSL
                     FAHLARVGGGEPDQVRLADLRTWLANLQAGGADRATLARRSAGARTFFAWARRTGRIA
                     ADPAATLRSPRVDRRLPPTIAVDHARDLLDGLAARIAEVDEPVARAGRQRDLAIVEVL
                     YSCGIRVSELVGLDLPDFDVQRGMLRVLGKGGKERTVPLGRPARRALEDWLSVRSQLV
                     APAARSAMFVGDRGGRIDPRVVRRLVHQALAQVPDAPDLGPHGLRHAMATHLLEGGAD
                     LRSVQELLGHSSLATTQLYTHISSERLRAAYRQAHPRSGAQG"
     gene            complement(10625..11557)
                     /locus_tag="PKJ61_09250"
     CDS             complement(10625..11557)
                     /locus_tag="PKJ61_09250"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOC14049.1"
                     /translation="MRRSRLLAGLVLAAGLLLHPVPAAAATAVPPIGGAVVRGFDPPA
                     QPWLSGHRGVDLLGKVGEPVLAVMAGRVTYSGTLAGRGVVVVSHGSLRTTYLPVTPGV
                     SVGAEVSAGEVIGGLAGGHTCPGGTCLHWGLKRGDEYLDPLSLLGDTALRLLPADAED
                     QVADIVRYRAAAIAEGGKLPGLLSSPADAEVGSKFGMRLHPIFHEWRLHNGVDLGAKC
                     GSPIRAAAPGRVVRVSYDSASGHRLEIDHGEVGGHHLRTGYLHATGYSVRVGQTVKRG
                     QMVGRVGTTGWSTGCHLHFITWLDGSYTDPERFL"
     gene            12017..>12157
                     /locus_tag="PKJ61_09255"
     CDS             12017..>12157
                     /locus_tag="PKJ61_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002527014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOC14050.1"
                     /translation="MAVVTTRQLLDSGVHFGHQTRRWNPKMKKFIFTERNGIYIIDLQ
                     QSL"
BASE COUNT         1975 a         4070 c         4197 g         1915 t
ORIGIN      
        1 ccgctcacat ccggatggcg atgcagctcc cggcgcagca gttgggcgtg cggcaggtcg
       61 gccgcgaggt gctcgtcgaa ctcggccagc aagcgaaccg ccggatcact tggccaatca
      121 gtcacccgct catcttggca gggtcggcac tcaagttggg agaggccgga agctcagcag
      181 ggtcacgtca acggtgatct cgccttcgga tgccacttcc ggtagccggt ggaaagcgtt
      241 cggacccatc gcgatcaggt cgagcgcacg ctcggcatcg acctgcgaag ctgcgctgat
      301 ccgaaccgac tccaccggct cgaatagccc ggcggccgcc tcggtgagct gttctgcctt
      361 gtcggcgtcc actttcaaca ggccgtaccg atcccggaga ccgtccaggt ggccggcatt
      421 gggaagcacc acgacgagcc gtccgtccgc ttccagaaca cgtcggaact cgggcaggtt
      481 cctgggcgcg aacacgcaga gcaccgtgtc caggcagcgg tcccggatgg gcaggttgcg
      541 ccagacgtcc gccaccaccg cgccgatcct cggatgggcc cggacggcag cgcgggcggc
      601 cgctttcgac acgtcgagcg cgataccgac cgcctccgga cgccgctcga gcgcgcgcgc
      661 caggtagaag ccgttgcccg caccggcttc aagcagccga ctgccgcgaa cctgcgctgc
      721 gacggcctcc gcaacttcgt caaaaagccc ggacgcatgc acgcgtccgc gggcctgcag
      781 catcgccggg gtgtcggcgt tggccggctc ggcaccaccc agcaggttca cgtggccctg
      841 gcgcgccagg tcgtagctgt gtccagccgg gcagcgcagc acccccggcg agagttcgag
      901 gtgctgcgca caaaccgggc atgcgagcag cgctaccgcc ctcgcgtcca tcccggccgc
      961 ccgaatcagc tggcgaggaa ctcgagaccg gtaccgtcgc cggccttcag ggtcagcgaa
     1021 gagccctcga taacgaagct ggtcgccttc tgcagcgtgg ccagaaccgc ggtctcctcg
     1081 gcggagaggg cctcgttggc gcacgccatc ttggtgctgc gcagcgggcc ggccttgaag
     1141 ctgccgtcat cggaggtctc gacggtgccg ctgaagttat tgcaggcttt gccgctgagt
     1201 tggccgtcct ggatcgtcat ggtcaccgag ctgccttcga ccggtgcggt cacggcgtcg
     1261 ttggtcacga tgccggagag cttccaggcg gtgccggcca gcggcttgtc gaccactggg
     1321 gcgagcacga ccagcacctg cgcgccggcg tccagaagtt cgaggccgga gtccacgctc
     1381 cgcaagttgg tcacggtggg gaccgtcccc aggaaggtct gctcctgggt catcaccgcc
     1441 gcgtcggtgc acatcatggc ggtgctggcg aacgtgtcga acttcaggtt ggctccgtcc
     1501 tgggtgaagc cgccggtgaa gcggttgcag ctggccaacc cggacacggt gctgccctca
     1561 aacaacatgg tcggctggtg gccgtccagg gtggcggtgc ccttgatgga cgtcaccgtc
     1621 caggaggtgc cgctcagggc gggagcggac gctaccgacg atgaagcggg ggccggcgcc
     1681 gctggcgtgg tggtgctgca cgcgctcaac agggcgacta ggccgacggt ccagatcgca
     1741 taccttctca tggtcatcct tcttcaactc ccgccgccgg ctccttgctc gagcgccgca
     1801 ttgcgcggac atcggagcgg atcagcagga aaaacccgac gccggccaac ccggcgaaca
     1861 tgaaccattg aagcgcatag gaaaaatggt ttccctcatt cagctcaggt ggctgcaccg
     1921 gaaccagtcc ggacggatcc ccgggcctcg tctcaatggc ggacaggtaa ccgttcacga
     1981 gttcgcggcc cagggcctgc gccagcgccg ttgaattcac caagcgaata gtttgctggg
     2041 tcggcgtcat cgcgtccgtg ctgccggtct cgtcgcgccg tacgtagccg acgatctcca
     2101 cctgcccgga cgggggcgcc ggcagcgact tcggatagtc ctggtcgggg ggcttcaccg
     2161 cgaaaccccg gtcgaccagc acccaggctc cgctggtggc ctgcagcggc gtgacgattt
     2221 cgtagccaga gtcggcgccc tggacccggt agcgcaccag gaactggttg gcggcgtcgt
     2281 aggtgccccg cacccgcacc cgctgccact ggtcggcctc ggtgatcggt cggttgaaca
     2341 cgtccgcgaa ctcccgcaac gggccggact cgtgctcgac caccgcgctg ttgcgttcgc
     2401 ggcgctggtc caggcgatcc aactgccagc ggccgaggtt cacgaaagcg atggcgagcg
     2461 cgaccacgaa gaccgccagc gcgacccaac gcagccaggg ccggcgaccg gacggagcca
     2521 cgcccatcgg cgtgttcagc agcttcattc ggtctcatcc tccacggtgc cgtcgacaac
     2581 ccccgggctc ggcagtgacg gacggtctga cggtgccggc gcggtcggcg cgggcggcgt
     2641 ccgctggtcg atggcgttgg ccagcatcac cgcgatggcc ggcagcactg cagcggctac
     2701 cagcagcagc aggttccagg gcaggggcgt gacggtggca gcgatgaagc aggccacccg
     2761 cacggccatc gtccacaggt aacggaagcg ccgggcgtcg gtgtccagcg aacggctcgt
     2821 ccgcgcgctg gtgatcagcg ccggcttgga atcgttgccc gggctcacca ccccagccta
     2881 ggctcccggg cccggcggct gacgaatcca gccccggggc ggaccggtgc acgacttccg
     2941 gcggggtgat ccgctagcgt tgcgccgtga ctgaaatctc aacaaaccca atcgcgcgca
     3001 gtgtggtcgt gaccggcggc aaccgcggca tcggccgggc catcgtcgag cgcttcctcg
     3061 cagccgggca caaggtggcc agcctagacc tgggcggcga ggcgccggcc ggtgcgatcg
     3121 gcatcaactg caacgtgacc gacgccgaac aggtcgacgc cgccttcgcc gcagccgagg
     3181 aggcgaacgg cccggtcgag atcctggtgg ccaacgccgg catcacgaag gacaccctga
     3241 tcctgcggat gagcgatgcc gactgggacg ccgtcctcaa cgtcaacctc agcggtgctt
     3301 tccgctgcgc gcgccgcgcg gcgaagagca tgatccgcca gcgcttcggc cggatcgtct
     3361 tcaccggctc ggtggtggcg ctgtacggct cggccggcca ggtgaactac tccgcgtcca
     3421 agtccgccct ggtgggcatg gcccgctcgc tgacccgcga gatcggcagc cgcggcgtca
     3481 ccgcgaacgt ggtcgcgccc ggcttcatcg aaaccgacat gaccgcagtg ctgtccgagg
     3541 acgtgatcgc gcgctacctc gcgtccattc ccgcggcgcg cctcggccag cccgaagagg
     3601 tggccgcagc ggtggcgttc ctcgcgggcg aggaggctgg cttcatctcc ggcgcggttc
     3661 tcccggtcga cggcggcctg ggcatggggc actgaaaggc atcgcatggg cattctcgaa
     3721 ggcaagaaca tcctggtcgc cggtgtgacc atgaacacct cgatcggcta ccgcgtggct
     3781 gagatcgccc aggccgaggg cgcgaacgta gtggtgtcga acttcggccg cgcgatgagc
     3841 cttaccgcgc gcgtgctgaa ccggctcgac tcgccgccgc cgctgctcga actggacgtc
     3901 accgacgaaa gccacctggc cagcctcgcc gatcgtctcg gtgaacactt cgaccgcctc
     3961 gacggcgtgg tgcactcgat cgctttcgcg aacccggaga ccgcgctcgg cggcaagttc
     4021 ctctccacgc cgttctccga cgtcggcttc tcggtcaacg tgtccgccta ctccctggtg
     4081 agtctggcga tcgcctgcaa gccgctgttc gcaccgtccg ccgcgatcgt gggcctgacc
     4141 ttcgacgccc ggacctcctg gccgatctac gactggatgg gcgtttccaa ggcggcactt
     4201 gagagcacct cgcgctacct cgcccgctat ctcggtcccg agggcgtccg ctgcaacctg
     4261 gtggcggcag gcccgctgga gacgatcgcc aaaaaggcca tccccggttc ggaggagttc
     4321 aacaacatct ggggcaagcg ggcgccgctc ggttgggacc cgaaggacct cacgcccacc
     4381 gcgaaggctg tggtggcgtt gctgagcgac tggttcccgg ccaccaccgg cgaaatgctg
     4441 catgtggacg gtgggctgca ctccacgggg gcctgagggt cgctaagttg ggcggtatga
     4501 acgtcgtcca actcgagggt gtctcgatca tccgatccgg ctccaccctt ctcgacaacg
     4561 tcagttggac ggtgcaggag gatgagcgct gggtcgtgat cgggcccaac ggcgccggca
     4621 agaccacgat gctgcaggtc ctgggcgccc aatggcatcc gtcccgcggc tcggcttcga
     4681 tcctgggtga gcggctcggc gcggtggacg tgttcgagtt gcgtccgcgc atcggcgtga
     4741 cctcggccgc gctggccgac cggatcccca agtcggagcg ggtttccgat gtggtggtct
     4801 cggccgcgta cgcagtgatg ggccgctggc gcgaggatta cgacgaactt gactatgagc
     4861 gcgccaggca actgatgcag cagttgcggg tcgaccatct ggccgaccgt actttcggca
     4921 ccctcagcga gggtgagcgt aagcgggtac agatcgcacg ggcgctgatg accgaccccg
     4981 aactgttgct gctggacgag cctgccgccg gcctcgacct ggccgggcgt gaggccctgg
     5041 tcaccactct ctcggagctg tgtacccacg aactgtcccc ggccaccgtg ctggtcaccc
     5101 accatgttga ggagattcca ctgggcgtca cgcacgcgct gctgctcaag catggccggg
     5161 tggtggcagc tggcccgatc gagaagaccc tcaccgggcc gctgctgagc gcgactttcg
     5221 acctgctgct ggaagtgagc aacgtcggcg gacgctgggc ggcacgggca gctgctcgtg
     5281 ccggccacca ccttcccact cggttggcat gatgggttcc agatcccgtc cgtaaaccga
     5341 gttgagggga accgatggct gagtggctcg ctgagcacgc ctgggctgcg tggatggtgc
     5401 tggctgccct gctggcgatg gccgaaatgt tgacccttga cttcacgctg ctgatgcttg
     5461 cggtcggcgc actggctggc gggctggtcg cattggcctt ccccgggctg atctgggtgc
     5521 agttcctggt ggccgttgtg gtcgcggtgg ccatgctcgg gttgctgcgg ccaacgctgc
     5581 tgcgcaagtt gcgtgatcta cccggctacc gctccagcat cgacaagatg gtcggcagtt
     5641 ccggggtggc gctcaccgag atcaccgcgt ccgcgggtga ggtgaaggtg gccggcgaag
     5701 tgtggacggc gcacagcctt gagggcacga tcgccgccgg caccgagatt gagatctacc
     5761 gcgtcgacgg cgctactgtc atcgtctacc cccgtcacct ggccctgccc tgaccccaga
     5821 ggagcaacaa atggagtacg tgatcatcgt cctatggctg ctggtggcgg ccgtcgttgt
     5881 caccgtgctg ctgcgcagcg tccggcagat ccggcaacag caggtggcgg tggtggagcg
     5941 gctgggcaag ttcaagcgca cgctggaccc gggcctgcac attctggtgc cgttcgtgga
     6001 cagcatccgc tacgtcatgg acatgcgcga ggctgtggtg ccgttcccgc cgcagggcgt
     6061 gatcaccgag gacaacctga tggtgaacat cgactcggtg atctactacc agatcgtcga
     6121 tccggtacgg gcggcgtacg aggcccagaa ctacatccag gccatcgagc agctcaccat
     6181 gaccacgctg cgcaacatca tcggcggcct cgacctggag cagacgctga ccagccgcga
     6241 ggagatcaac cagaagctgc gcacggtgct ggacgaggcc accggcaagt ggggcatcaa
     6301 ggtgaaccgg gtcgagctgc gctcgatcga gccgccggcc acgatccgcg acgcgatgga
     6361 gaagggcgcc cgcgctgagc gcgacaagcg ggccgccatc ctcaccgcag aaggccagcg
     6421 gcagtcgcag atcctgtcgg cgtccggtga ccgcgaggcg tccatcctgc gggcgcaggg
     6481 tgaccgcgag gcccaagtac tgcgggccca ggccgaccgg caggcgtcca tgctgagggc
     6541 agaaggtgag gcgcaggcga tcacgacgac cttcgccgcg attcacgccg ggaagcccag
     6601 ccagtcgctg ctggcctacc agtacatgcg gatgctgccc aacctggccc gcggcgagtc
     6661 gaacaaggtg tggatcatcc cgagtgagct gaacgacgcg ttgaagggtc tcggccaggt
     6721 ggccgggaat gcgctgggcg cccgcgtggg tgcggagccg gaggcagcgg aaggcgagtt
     6781 cgaggcaccg gcacgggtgg acgtcttcgc cgagatcgag gagcagaaga ccaaggacga
     6841 agcggactcg gccaagtcgg tgcagcaggc gatcgccgaa gccgaagccc tggagggacg
     6901 caaagccgcc aggctcgccg tcgagaacca gaacgcgctg acccgggggg ttccggccgc
     6961 ggaaccggtc cagcaggccg agcccgaagc cgagcctgag cccgcctagg actcaatcgc
     7021 ccggtgcgac cttggcgtag cgtccggcca gcgctgagca ggccatgagc tgcgcctggt
     7081 ggaagatgac cagcggcagc atcagcaggc cgagcggttg tccggcgaag agcacgccgg
     7141 ccatgggcag accgctggcc agtgacttct tggtgccgca gaactggatc gcgatcgcgt
     7201 ccgctctgct gaagccgagc cggcgcgccg cccaccacgt tagcgcgagc atgccggcga
     7261 ggatcagcag gctgagcacg accagccggc tcagctcgac caccgaggtc tgtgcccaca
     7321 ttccctcgcg catgccggcc gagaacgccg aatagaccac caggatgatc gagccgcgat
     7381 ccaccagctt cagttccgga tgggcgctga cgaagccggc cgtccaccgt ctggagagct
     7441 ggccgagcac gaacggtagg aagatctgca gcacgatctc gacgatggtc gaggcggtgt
     7501 tgctgatctg gccggtgttg ctgagcagca gccagcacag caggggagtg aggaacactc
     7561 ccaggatgtt ggatgcggac gcgctgacga ccgcgccggc cacattgcca cgggcgatgg
     7621 acgtgaaggc gatcgaagac tgcacggtgg agggcaccag ggtcaggtag agcaggccgg
     7681 cctgcagccc cgggctgagc aggccagggg tgagcgccac cagcgccagg ccgagcaacg
     7741 ggaataccac gtaggtgaag ctcaggatgg tggtgtgcaa ccgccaatgg cgtagtcccg
     7801 caagcgtttc ggccgggctg agccgcgcgc cgtagaggaa aaagagcagt gcgatcgccg
     7861 cggcagtgaa ccagtccagc gcgtccgccc agaacccgct cgccggcagg atcgacgcca
     7921 ccaccgcagc gaggatgatc gcgagcagaa accgatcgat tcggatgcgg gagagccagg
     7981 acatttgcct catcctaagc tgcctagaat cacgccatgg ccaccctgat acaccttgac
     8041 cgggccgacg acgagcgcct cggcgactat gtgggcctgc gcgaagcgac cttgcgccgg
     8101 ctgctggaga ccgagcgcgg ggtgttcatc gccgaaggca gcaaggtgat ccgacgagcg
     8161 atcgaggccg gctatccgcc caggtcgttc cttctggccg agcgttggct ggccgacctt
     8221 gcggaccttc tcgacggctt ggacgtgccg gtgtacgtga ccactgaaga agtggccgag
     8281 gcggtgaccg ggttccatgt gcatcgcggt gcgctggctt cgctgtatcg cgcagagcgg
     8341 ttcacgatcg cggacgtgct cgccgggcgc cgggtgatgg tgctgcagga tctcgtggac
     8401 cacaccaacg tcggcgcggt gatccggaat gcggccgggc tgggctggga cggggtgctg
     8461 gtgagcgcgc gctgcgcaga cccgctctac cggcgttcga tcaaggtttc gatggggacg
     8521 gtgttcgcgt tgccttgggc acggctgccc gaatacgtcg atcttgccgg ggtgctgcat
     8581 tcggccggtt tcacggtggc cgcgctggcg ttgcgtccgg acgcggtcga actggccgac
     8641 ttcgctgctg gcctggccaa agaagccagg gttgcagtgc tgctcggcac tgagggccac
     8701 ggtctcagtg cgggttggac cgacgccgcg gacgcggtgg tgcgaatccc gatgaagtcc
     8761 ggggtggact cgctgaacgt ggctgctgcc agcgcgatcg cgggctacgc gcttggttag
     8821 ggaaccgctt ttcaacgctg gctgagcctg acgaagcccg tagtcaccgc gcggatgtag
     8881 gacccaacgc ccggctcagg gatcgttgct cactgatcgg ccagactctg ggggattcgt
     8941 cgggcccggc caccgatggg ctcagccgtc ggccgaggtt cgcttcgggg tgagcggcca
     9001 atcgtcgggg tagaggtggg cgagttcctc gtgctgggcg tagagcttgc ggcgcaggcg
     9061 cccggacaga cgatcgccga agaccgtgcc gttcaggtgg tcggtctcgt gctgcaggca
     9121 gcgcgccagc atgccggtgc cctcgatggt gaccgggttg ccccaagggt cgacgccggt
     9181 gcagattgcc ttgtctggcc gggccagggg ctggtagccg ccgggcaacg acaggcaccc
     9241 ctcgtcctcg gatacgaggt tccgctggtc gccctcggga gtggtcacca caggattgca
     9301 gaccgcgccg atctggtgga tctcgtccgc gtccgggcag tcgtagatga agacggccaa
     9361 atccacaccc acctgggtgg cggccaggcc gacgccgtgg gaggcagcca tggtggcgaa
     9421 catgtcccgc accagctccc ggagttcgtc gtcgaaggcg gtgaccgccc gtgtctgggc
     9481 gtgtagcacc ggctcgctcc agcgggtgac ccggcgcagt ttcccgccgg tggtcagctc
     9541 ttcaatgccc atgcgtcctc actcttcgtc ggctgcccat cttagggctc accgcgtggc
     9601 cgcttgcgca gtgtcctctc cgaggtgacg ctggcggggt gactgcgagc gaggacttcc
     9661 tcacgccggc agctgctcgg atggtgactg aattcgccgg gcacctgcgc ctggagcgtg
     9721 ggctctcacc gcataccgtc cgcgcctaca cctcagatgt gacgagtctg ttcgcccacc
     9781 tagcgcgggt cggcgggggc gagcccgatc aggttcggct cgccgatctg cgcacctggc
     9841 ttgcgaacct gcaggcaggc ggagccgacc gggccacgct ggcgcgccgg tcggccgggg
     9901 cacgcacctt cttcgcctgg gcaaggcgga cgggccgaat cgctgccgat ccggccgcga
     9961 cgctgcgttc cccgcgggtg gatcgacggc tgcccccaac catcgcagtg gaccacgccc
    10021 gtgacctgct ggacggactt gcggcgcgga tagccgaagt cgatgagccg gttgcccgtg
    10081 ccggccgcca acgcgatctg gcgatcgtcg aagtcctcta ctcatgtggc atccgggttt
    10141 cggagttggt gggtttggac cttcccgact tcgacgtgca gcgcggcatg ctccgcgtgc
    10201 tcggcaaggg cgggaaggaa cggacggtgc cgctcgggcg gccggcgcgg cgggcgctgg
    10261 aggactggct gtcggtgcgg tcccaactgg tcgcaccggc ggccaggtcg gcgatgttcg
    10321 tgggcgaccg tggcggccga atcgatccgc gcgtggtccg ccggctggtg caccaggcgc
    10381 tcgcgcaagt gccggacgcc ccggacctcg gcccccatgg tttgcggcac gccatggcca
    10441 cgcacctgct ggagggcggc gccgacctgc gttcggtgca ggagctgctc ggccactcct
    10501 cgctcgcgac cacccagctc tacacgcata tcagcagcga gcggctgcgc gccgcctatc
    10561 ggcaggccca cccgcgctcg ggtgcgcagg gctgaggagg ttgtgtccgc cgccgcatcc
    10621 atggctacag gaagcgctcg gggtcggtgt aactgccgtc cagccaggtg atgaagtgca
    10681 ggtgacagcc ggtactccag ccggtagtgc ccacccgccc taccatctgg ccgcgtttca
    10741 cggtctggcc aaccctcacc gagtagcccg tagcgtgcag atagccggtt ctgagatggt
    10801 ggccgcccac ctcgccgtgg tcaatctcca gccgatgccc gctcgccgag tcgtagctca
    10861 cccgcaccac ccgtccgggc gcagccgccc ggatcggcga accgcacttc gcacccaggt
    10921 ccacgccgtt gtgcaagcgc cactcgtgga agatcggatg cagtcgcata ccgaacttag
    10981 agcccacctc cgcgtccgct ggactggaga gcagaccggg tagcttgccg ccctcggcga
    11041 tcgcggcagc tcgataacgc acaatgtcgg ccacctgatc ttccgcatcc gccggcagca
    11101 gccgcagtgc cgtgtcgccg agcaggctga gcgggtcgag atactcgtcg ccgcgtttca
    11161 ggccccagtg caggcaggtg ccgcccgggc aggtgtggcc gccggcgagc cccccgatca
    11221 cctcgccggc ggacacttcg gcccccaccg aaacccccgg cgtgaccggc agataggtgg
    11281 tgcgcaggct cccgtgggag accaccacca ccccgcgccc ggccagcgtt ccggagtaag
    11341 tgacccggcc agccatgact gccagcaccg gctcgccgac cttgcccaac aggtcgacgc
    11401 cgcggtggcc agacagccat ggctgggccg ggggatcgaa gccgcggacc accgcgcccc
    11461 cgatcggagg aaccgcagtg gccgcagcgg caggcaccgg atgcagcagc aaccccgccg
    11521 cgagtaccag cccggccaac aggcgcgatc tcctcatgcc ctatttatcg tcacgggagc
    11581 cggatttgtg tcacccgatt tgttgaactg tggataactc agtggttgtg gacaactcct
    11641 agctgtcttc accctgtacc agtccaggta ccgacccggt gttgctcggc gatgttccag
    11701 acggacaatt tgcccaggat tcgaccggaa ttgggctcag tgctgccgat tgggacatcg
    11761 acggggcgtc ttgctacacc gaaccccgat tggcgagcgc ccgtccgccc ggcgtagact
    11821 cagggcagcg acccggccga tccgggtcga cttcgcacgc agcctcaccg ccgtacggcg
    11881 ggtcggcgcc cgtggtcccg aatcagcccc ggctggacgg gtgggaatcc ggcggctgcg
    11941 tcaggcggac cgaaaagctc ggtccggaca acccaagcgg gtcgggctca gcctgcccgc
    12001 gcagagagga acggccatgg cggtcgtaac cacgcgccag ctgcttgaca gcggcgtcca
    12061 cttcgggcac cagacccggc gctggaaccc gaagatgaag aagttcatct tcaccgagcg
    12121 caacggcatc tacatcatcg acctgcagca gtcgctg
//
LOCUS       DAOPRQ010000068        12077 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_4458_length_12077_cov_12.722010, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000068 DAOPRQ010000000
VERSION     DAOPRQ010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 12077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12077
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4458_length_12077_cov_12.722010"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="PKJ61_09260"
     CDS             complement(<1..224)
                     /locus_tag="PKJ61_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14051.1"
                     /translation="MPGEVAMQAWSRRLLPVLALVVAVGCTPAAPTPSPLPSFRCTPE
                     AGGAEFDCSQAQYDEMVAKDALYAEAEAVYR"
     gene            complement(359..1069)
                     /locus_tag="PKJ61_09265"
     CDS             complement(359..1069)
                     /locus_tag="PKJ61_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HOC14052.1"
                     /translation="MTALLGLATRLGLARLMLITGTRSGQGDLAEFVDAAFAGGVDAV
                     QVRDPQATQPELLAALNAVRSVGYRYQGLVCAYESGPLAQEFQADLLHLPERGMGARL
                     ARGFLHKWALIGRSCHSRAEIDAALADADVNYLTIGPAFGGLGGSSAGLSLVRYAASV
                     APTGDRESKPWFAVGGVTAANLDEVLAAGARRVAVTRAITEAADAEAAAMAMKDRLRQ
                     AWNEDPEMQGLTLKVFRD"
     gene            1187..3181
                     /gene="pknB"
                     /locus_tag="PKJ61_09270"
     CDS             1187..3181
                     /gene="pknB"
                     /locus_tag="PKJ61_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HOC14053.1"
                     /translation="MNNTDPLVGHVVDGRYEILRKLARGGMATVYLATDRRLTRTVAI
                     KVMHDNLGSDQDFVSRFDREARAAARLSHPNVVSVFDQGMDAGRPYMVMEYVEGSTLR
                     NVITREAPMEPQRALDLLLPVVAAVAAAHEAGIIHRDLKPENVLISGRGQIKVADFGL
                     ARAVTAHTATANGMLIGTVSYIAPELVTQGHADTRCDVYALGVVLFEMLTGRKPHTGD
                     TPIQVAYSHVHNEITAPSASAPAIWRDSRSAIPPYLDALVLAAAARQPADRPADARVL
                     LDHLRQAREAIERGVSDDPALTARMRETRFDPTSSVTEVVPTIAAEPEVHRTATLRFT
                     PSTPISPSHPGAADGMPYYDAPAPVSPSAAPLVERRVRKRRSGVVSLVLLLLVTAILG
                     VGAWYLLAGRFTATPDFDNLDRAAATKLAAKHGLQLVFNEEFSETVPAGQVTRTEPKA
                     GDRVVIGGSVTAFLSKGQERFPVPSLVGRTVDDAISALKESNLEVGKTPEVYDEKVEI
                     GSVVSASIEAGQPVKRGTVVDLNVSKGPAPVKIISYVGKTFDEAKAFYEKAGLVVARA
                     PEDKFSTKIAAGSIISQDPKKGTLERGKTITFTVSKGPEMIAVPNVRGKATEEAKKIL
                     TDAGFKVEVEQQFWSGFALGTRPGEGELAPKGSTVTLVVG"
     gene            complement(3195..3836)
                     /locus_tag="PKJ61_09275"
     CDS             complement(3195..3836)
                     /locus_tag="PKJ61_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002513957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOC14054.1"
                     /translation="MQITHLGHSAVLVETPAIRALIDPGNFSDAWHGLTDLDVIAVTH
                     LHPDHVDPQHVPALIAANPLARVLVEPGVMDAVPLERAEPITADTAVSFGPVSIAAVG
                     GLHAVIHRDIPQVGNVGLVISAEGEPTFFHPGDSLAAIPAGVDVLAVPAYGPWAAMKE
                     TVDFAREVGAVEGFAIHDGLLNERGWSLVFNRVREMTPCQLTDLRDRRAWTPA"
     gene            complement(3856..4317)
                     /gene="aroQ"
                     /locus_tag="PKJ61_09280"
     CDS             complement(3856..4317)
                     /gene="aroQ"
                     /locus_tag="PKJ61_09280"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007079492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HOC14055.1"
                     /translation="MDVLVINGPNLNTLGTREPGIYGATTLAEIEAALVAQGAASGLS
                     VDTFQSNHEGAIIDRLQAASADGTRFIIINPGAFTHTSVAIRDAFAAVAIPFCEVHIS
                     NVHAREEFRRHSYLADLAVGVMAGFGVRGYSMALDYVAATLAGGSGQPAMA"
     gene            complement(4323..4823)
                     /locus_tag="PKJ61_09285"
     CDS             complement(4323..4823)
                     /locus_tag="PKJ61_09285"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HOC14056.1"
                     /translation="MIVLIGFMGAGKSTVGRLLAAKLGLPFADADALIEAEQGLSIPD
                     IFEGYGEPEFRRIEAETITKRLAGPETVLSLGGGALGTTAVRDALAGHQVVLLDVELA
                     EALRRVGGDGSRPMLARNDVPELYASRQQAYRAAAKLVVPVTGRTPQEVAASVLAALV
                     APGVGE"
     gene            complement(4820..5701)
                     /locus_tag="PKJ61_09290"
     CDS             complement(4820..5701)
                     /locus_tag="PKJ61_09290"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008356820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HOC14057.1"
                     /translation="MRAAVLGSPIAHSLSPVLHTAGYAALGLTDWTYDRFELVAAELP
                     GFLSGLDASWRGLSLTMPLKTVCLEVAEVVTDRARRAGVGNTLVRLPDGGWLADNTDV
                     AGLVSALTPSWQPEWTHAAVLGAGATARSALLALAELGVREVTVYARTPAKAEPLIEW
                     APTELTVSVRPLAAWTPDAEPAVLSTLPAGAADDLPAPNVIPAAHQPNVIPAAHQPNV
                     IPAKAGISPRLLFDAVYANWPTPLASWAQAANWTVVGGLDLLVHQAALQFELFTGHQA
                     PIEAMFAAGRAALEQPK"
     gene            complement(5698..6723)
                     /locus_tag="PKJ61_09295"
     CDS             complement(5698..6723)
                     /locus_tag="PKJ61_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017933891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HOC14058.1"
                     /translation="MIDLRSDTLTKPTPGMLAAMASAEVGDDVYGEDPTLNAFEARVA
                     ELLGHEAGLFCTTGSMANLLGVWCLVPPGQEVVADVRAHIVRAELGAHAALHGITTRT
                     WQSADGIADAATIADLITPDAGPYLVSTAAVALENTHNFGGGTVQPYSTLVEVNRLCR
                     DAGVGLHLDGARLWNAHVATGVALADYGRLFDTVNVCFSKGLGAPVGSMLVSSAANIA
                     KARAQRKRLGGGWRQAGVLAAAAEYALDKHLPRLAEDHVAARAFAAAVAESAPDAVDV
                     GAVQTNIVVLDAGARAAAEVIPAVLARGVGLSAVGSRTLRAVTHQEISVDAARAAGQI
                     AGEVLAG"
     gene            complement(6725..8113)
                     /locus_tag="PKJ61_09300"
     CDS             complement(6725..8113)
                     /locus_tag="PKJ61_09300"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002513958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HOC14059.1"
                     /translation="MLEPIPSLAELHALGAAQQPSYPDPDAVARVLAELRRRPPLVFA
                     GECDVLRDKLADVAAGRSFLLQGGDCAETFESVTAANIRGKLRVLLSMAVVLTYAAQV
                     PVLKVGRIAGQYAKPRSRDSETRGATTLPAYRGDAVNALAFEPEARQPDPQRLLEVYN
                     YSAATLNLLRAFVKGGFADLRAVHTWNADFVHNSNVEERYEHLADEISRALAFMVACG
                     VDKESFRTVDFYSSHEALLLEYEHALTRIDSRTDTPYNTSAHMVWIGERTRQLGGAHV
                     EYLRHLRNPIGVKLGPSAVPSEVTALFDKLDPDHEPGRLTVISRMGAAKVRDALPPIV
                     EAVEQTGRKVAWVCDPMHGNTFETSLGYKTRAFSQVADELNGFFDVHEGLGTWPGGVH
                     IELTGDDVTECVGGVDELAEADLANRYETACDPRLNRNQSLELSFLIADRLTDGRIRR
                     VNPVQEYSAEDF"
     gene            complement(8119..8877)
                     /locus_tag="PKJ61_09305"
     CDS             complement(8119..8877)
                     /locus_tag="PKJ61_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014847069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOC14060.1"
                     /translation="MYYALFKNVLFAPIVRWLFRATIEGEENLPKTGGVILASNHLAA
                     GDTFVMPAMLHRQVTFPAKAELFAGNRGIGSKIVAWFLKAVGQVPLDRSGGRVSLEGL
                     GPVLRVLADGGMVGIYPEGTRSPDGRLYKGRTGVARLALAAGVPVVPVGMLNTQTVRR
                     LGIPWIWKPQVKIGKPMDFGAYAGLHDDRETLRWVTDEVMNAIMELTGQTYVEAYGTS
                     LKSGSLTAADAEARVLPRPGYGATPPELPSGGLS"
     gene            8900..9673
                     /locus_tag="PKJ61_09310"
     CDS             8900..9673
                     /locus_tag="PKJ61_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007078096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOC14061.1"
                     /translation="MPSTPTTPRVGDYAQPFSADGGRIGVLFCHGFTGSPWSLLEWAK
                     LTADAGYSVSLPRLPGHGTTWQELNLTTWRDWYDTVEAAFLELRSRCDVVFVAGLSMG
                     GALALRLAERHRDEVAGLVLVNPAVVGMRRMAAIPLLKLFLPSAKAIGSDLIDTKVDE
                     HAYDRTPLKAAHSMMKMWADIRACMDLVICPVLIFRSATDHVVPAASTAFVLSHASSE
                     DISERVLENSFHVATMDRDKEQIFTESLAFFAKHSAKHP"
     gene            complement(9679..10500)
                     /locus_tag="PKJ61_09315"
     CDS             complement(9679..10500)
                     /locus_tag="PKJ61_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14062.1"
                     /translation="MGRCPPGDRLTLRARLAAAAVAFALVGCQPTPPIATIPEPVEGP
                     ESTSTEFGSLTASRLSETDRAAWTQRIEQAARTVRAAEITAAVRPGWQGKLDVVIATD
                     AAEFASRTATPADAASAATTCTDGASEIVVNPVVLVAGDPWLRSTLVHEGVHVATASA
                     CVPSGKSLGWATEGLAESVAAGADPSTASRNHKLVIDYLASHALPDKLPSELNTLTDY
                     ALAQLAVDQVIKHRGRQADDFLARAIHQAAKVTEAEHEQVRSWYVAELRRLRDGG"
     gene            complement(10470..11528)
                     /locus_tag="PKJ61_09320"
     CDS             complement(10470..11528)
                     /locus_tag="PKJ61_09320"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HOC14063.1"
                     /translation="MQAGRHARTALAVAAATLVVVAGPFADPASAVPTPTPTPTPSAQ
                     PIAAKIKDAKAQVEELEKQVSEVGEDYEVAAAALEENKDRLKSLKSDISRQQKLVDTL
                     AEQVRVIALRQFQNRDVDTTVQIFTSGDPDTLLDRLSTANKVDQDMTDTLAQHEAEQA
                     NLTDMTSTADAEVAALAEQEQKLAAMKKDLQDKVDAAQALVLQLTDQQRAELEAAEGG
                     RSTFDASELDETTVNAKIIAAIKYATSKVPNGQYVWGSSGPTGFDCSGLMLAAYRSVG
                     IGLPHSSRAQANVGRAVSKDELKPGDLIFWYHPIHHVGMYIGNGKIVHARNTRSDLVI
                     QTLASYPAPWAGARRVIG"
     gene            complement(11616..11849)
                     /locus_tag="PKJ61_09325"
     CDS             complement(11616..11849)
                     /locus_tag="PKJ61_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14064.1"
                     /translation="MDVLHVDCATCAARGPACADCVISVLLGPMPAGLTLDDTEQVAL
                     AAMADSGLLPPLRLVPTPPTWEQERVAKLRAVP"
BASE COUNT         1925 a         4210 c         4053 g         1889 t
ORIGIN      
        1 cgatacaccg cctcagcttc ggcatagagc gcatccttgg cgaccatctc gtcgtactga
       61 gcttgggagc agtcgaactc ggcgccacca gcctcggggg tgcagcggaa cgatggcagc
      121 gggctcggcg tcggtgcggc aggcgtgcaa ccgacagcca ccaccaacgc cagaaccggc
      181 aacagccggc gcgaccaagc ctgcatcgcc acctccccag gcatcaggag tctggcacac
      241 cgcccaacca agaaccagcc ctttgatgat ttatccacag gctcaagcga agttcgtaca
      301 cttgtccaca attcgcacac gcgtcacggc agagaattcc gcccaaccgg ttaggcagct
      361 agtcccggaa caccttcaag gtcagtccct gcatctcggg atcctcgttc caggcctgcc
      421 gcaggcgatc cttcatggcc attgcggcag cctccgcatc cgctgcctcg gtgatcgccc
      481 gggtcaccgc cacccgacgc gccccggccg ccagcacctc gtccaggttc gcggcggtca
      541 ctcccccgac cgcgaaccat ggcttcgact cccgatcacc cgtcggcgcg accgaggccg
      601 cgtaacgcac cagcgaaagc ccagcgctgc tgccgccaag cccgccgaac gccggcccga
      661 tggtcaggta gttcacgtcc gcgtccgcca gcgcagcgtc gatctcggcg cgggagtggc
      721 aggagcgtcc gatgagcgcc cacttgtgca ggaagccgcg cgcgagccgc gcacccatgc
      781 cgcgctcggg caggtgcagc aggtcggcct ggaactcctg cgccagcgga ccggactcgt
      841 acgcgcagac cagcccctgg taccgatacc cgaccgagcg gacggcgttc agcgcggcca
      901 gcagttcggg ctgggtcgcc tgcgggtcgc gcacctgaac cgcgtccacc ccgccggcga
      961 aggcggcgtc cacgaactcg gcaaggtcgc cctgcccgga gcgggtgccg gtgatcagca
     1021 tcaaacgcgc gagtcccagc cgggtcgcca atcccagtag cgcagtcacg gcgccagcct
     1081 aaccagcggc gggtcgatgc gggtgaactt ggtcccgacc tctacaatcg cctctaggtc
     1141 tggacggccc acttcctcga cttgtccgga gcaaaaagtg acgaccatga acaacaccga
     1201 tcccctggtg gggcatgtgg tggatggtcg ctacgagatt ctccgcaagc tggctcgtgg
     1261 cggcatggcc accgtttacc tcgccaccga ccgccggctc acccgaaccg tcgcgatcaa
     1321 ggtgatgcac gacaatctcg gcagcgacca ggacttcgtc agccgcttcg atcgcgaggc
     1381 gcgtgccgcc gcccggttgt cgcaccccaa tgtggtttcg gtgttcgacc agggcatgga
     1441 cgccggccgt ccctacatgg tcatggagta cgtcgagggt tcgaccctgc gaaacgtgat
     1501 cacgcgcgaa gccccgatgg agccgcagcg cgccctcgac ctgctgctgc cggtggtcgc
     1561 tgcggtcgcc gccgcgcacg aggcgggcat catccaccgc gatctcaagc ccgagaacgt
     1621 gctgatctcg ggacgcggcc agatcaaggt ggccgacttc ggcctcgccc gggcggtcac
     1681 cgcgcacacc gcgaccgcca acggcatgct gatcggcacc gtctcctaca tcgcgcccga
     1741 actggtcacc cagggccacg cggacacccg ctgcgatgtc tatgcgctcg gcgtggtgct
     1801 attcgagatg ctcaccggac gcaaaccgca caccggcgac acccccatcc aggtggcgta
     1861 ctcgcacgtc cacaacgaga tcaccgcgcc gtcagcgagc gcgccggcga tctggcggga
     1921 cagccggtcc gccattccgc cctacctgga cgcactggtg ctggccgcgg ccgcgcggca
     1981 gcccgcagac cgtccggcgg acgcccgggt gctgctcgac cacctgcgcc aggcccggga
     2041 ggcgatcgaa cgtggcgtga gcgacgatcc ggcgttgacc gcccggatgc gcgagacccg
     2101 gttcgacccg accagttcgg tgaccgaggt ggtaccgacc atcgcggccg agcccgaggt
     2161 gcaccggacg gcgaccctgc ggttcacccc gtccacgccg atcagcccga gccatccggg
     2221 cgctgcggac gggatgccct attacgacgc acccgcgcca gtcagtccct cggcagcgcc
     2281 gctggtggag cgccgcgtcc gcaagcgtcg gagtggtgtg gtcagcctcg tgctgttgct
     2341 gctggtcacg gcgatcctgg gcgtgggcgc ctggtatctc ctcgccggac ggttcaccgc
     2401 cacccccgac ttcgacaacc tcgaccgcgc ggccgccacc aagctggcgg ccaagcatgg
     2461 cctgcagttg gtgttcaacg aagagttcag cgagaccgtc ccggccggac aggtaacccg
     2521 caccgagccc aaggccggtg accgggtcgt gatcggtggc tcggtgaccg cgttcctctc
     2581 caagggccag gagcgtttcc cggtcccgtc cctcgtcgga cggacggtcg acgacgccat
     2641 ctccgcgctg aaggagagca accttgaggt cgggaagacc cccgaggtct acgacgagaa
     2701 ggtggagatc ggctcggtgg tgagtgcgtc gatcgaggcg ggccagccgg tcaagcgcgg
     2761 gacggtcgtc gacctcaatg tctccaaggg cccggcgccg gtgaagatca tcagctatgt
     2821 cggcaagacc ttcgacgagg ccaaggcgtt ctacgagaaa gctggtctgg tcgtcgctcg
     2881 cgcacccgag gacaagttca gcaccaagat cgcagccggc agcatcatct cccaggaccc
     2941 gaagaagggg accctcgagc gcgggaagac gatcacgttc accgtgtcca agggcccgga
     3001 gatgatcgcc gtcccgaacg tgcggggcaa ggccaccgag gaggccaaga agatcctcac
     3061 ggacgccggc ttcaaagttg aggtggaaca acagttctgg tccggcttcg cgctgggcac
     3121 ccgtcctggt gagggcgagc tggcacccaa gggatcgacc gtgaccctgg tggtcggctg
     3181 acgtccccaa cgcgtcaggc gggcgtccag gcccttcgat cacgcaggtc ggtgagctgg
     3241 catggggtca tctcgcggac gcggttgaac accagcgacc agccgcgctc gttgagcagt
     3301 ccgtcgtgga tcgcgaagcc ctccaccgct ccaacctcgc gggcaaagtc gaccgtctcc
     3361 ttcattgctg cccagggtcc gtaggcgggc accgcgagca cgtccacgcc tgccgggatc
     3421 gcggccaggc tgtcgccggg gtggaagaac gtcggctcac cctcggctga gatcaccagg
     3481 ccgacgttgc cgacctgcgg gatgtcccgg tggatgaccg cgtgcagacc gccgaccgcg
     3541 gcgatcgaca ccggcccgaa gctcaccgcg gtatctgccg tgatcggttc ggcccgctcc
     3601 agcggcaccg cgtccatcac ccctggctcc accagcacgc gcgccagcgg gttcgctgcg
     3661 atcaacgccg gcacatgctg cggatcgaca tggtccgggt gcaggtgcgt caccgcgatc
     3721 acgtccaggt cggtgagccc gtgccacgcg tccgagaagt tgcccggatc gatcaatgcc
     3781 ctgatcgccg gcgtttcgac gagtactgcg gaatggccga gatgggtgat ctgcatggtt
     3841 cgagcctacg tccgctcagg ccatcgcggg ctggcccgac cctccggcca gagtcgccgc
     3901 tacgtagtcg agggccatcg agtaaccacg gacgccgaac ccagccatca ccccgaccgc
     3961 aaggtcggcc aggtacgaat gtcgccggaa ctcttcgcgg gcatgcacgt tcgagatgtg
     4021 cacctcgcag aacgggatcg cgactgcggc gaacgcatcc cggatcgcta cggaggtgtg
     4081 cgtgaacgcc cccgggttga tgatgatgaa ccgggtgccg tcggcgctcg cggcctgcag
     4141 ccggtcgatg atcgcgccct cgtggttgct ctggaaggtg tccacgctga gcccggacgc
     4201 ggcaccctgc gccaccaggg cggcctcgat ctcagcgagc gtggtggcac cgtagatgcc
     4261 cggctcgcgg gtgccgagcg tgttcaggtt gggtccgttg atcaccagca cgtccatgcg
     4321 gcctactctc ccacgcccgg cgcgacgagt gcagccagga cgctggcggc gacctcctgg
     4381 ggtgtgcggc cggttaccgg gacgaccagt ttggccgcgg cccggtaagc ctgctgcctg
     4441 gacgcataca actcgggcac gtcgttcctc gccaacatcg gacgggaacc atcaccgccg
     4501 acgcggcgca gcgcctccgc gagctcaaca tcgagcagca ccacctggtg tccggcaagc
     4561 gcatcgcgca ccgcggtcgt gccgagggca ccaccgccca gcgacagcac ggtctccggc
     4621 ccagccagcc gcttggtgat ggtttcggcc tcgatccggc ggaactcggg ttcgccgtag
     4681 ccctcgaaga tgtcgggaat gctcaacccc tgctcagcct cgatcagcgc gtccgcatcg
     4741 gcgaagggca ggcccagttt tgcggccagc aggcggccca ccgtggactt gccggcgccc
     4801 atgaaaccga tcaggacgat cacttgggtt gctccagggc ggcccggccg gcggcgaaca
     4861 tggcttcgat cggagcctga tggccggtga acagttcgaa ctggagggcg gcctggtgga
     4921 cgaggaggtc caggccgccg actaccgtcc agttggccgc ttgtgcccag ctggctaggg
     4981 gtgttggcca gttggcgtag accgcgtcaa agagcaggcg gggcgagatc ccggccttcg
     5041 ccgggatgac gttgggttga tgcgccgccg ggatgacgtt gggttgatgc gccgccggga
     5101 tgacgttggg tgccgggagg tcgtcggcgg cgccggccgg gagcgtggac aggaccgcgg
     5161 gttcggcgtc cggggtccag gctgcgagcg ggcgcaccga aacggtcagt tcggtaggag
     5221 cccactcgat cagcggctcg gccttcgccg gcgtgcgcgc atagaccgta acctcgcgaa
     5281 cgccaagttc agccagggcc agcagggccg agcgggcggt cgccccagcg cccaggacgg
     5341 ccgcgtgcgt ccattccggc tgccaggaag gcgtcagtgc cgaaaccagg ccagcaacgt
     5401 cggtgttgtc cgccagccag ccgccgtccg gcagccgcac cagggtgttg cccactccgg
     5461 cccgccgcgc ccggtcggtc accacctcag ccacctcaag acaaacggtc ttcagcggca
     5521 tggtcaggct caggccgcgc cagctggcgt ccagccccga aaggaacccg ggtaactccg
     5581 cggcgaccag ttcgaaccgg tcgtaggtcc agtcggtcag gcccagtgcg gcgtagccgg
     5641 ccgtatgcag caccggcgac agcgaatgcg cgatcggcga accgaggacg gcggccctca
     5701 ccccgccagc acctcgccgg ctatctgccc agccgcgcgc gccgcgtcca ccgaaatctc
     5761 ctggtgggtg accgcacgca gggttcgcga cccaaccgcg gacaacccga cgccgcgcgc
     5821 gagcacagcc ggaatcactt cggccgcggc tcgcgccccc gcgtccagca ccacgatgtt
     5881 ggtctgcacc gcacccacgt ccacggcgtc cggcgccgac tctgccaccg ccgccgcgaa
     5941 cgccctagcc gcaacgtgat cctcggccaa ccgcggcaga tgcttgtcca gcgcgtactc
     6001 cgcggccgcg gccagcaccc cggcctgccg ccagccccca cccagccgct tgcgctgcgc
     6061 ccgcgccttc gcgatgttgg cggccgagga caccagcatc gagccgaccg gcgcgcccag
     6121 ccccttcgaa aagcacacgt tcacggtgtc gaacagccgt ccgtagtcag ccagggccac
     6181 cccggtggcc acatgcgcgt tccacagtcg cgcgccgtcg aggtgcaaac cgacgcccgc
     6241 gtcccggcaa agccggttca cctcgaccag ggtggagtac ggctgaaccg tgccgccgcc
     6301 gaagttgtgg gtgttctcca gcgccaccgc ggcggtcgag accaggtacg gaccggcgtc
     6361 cggtgtgatc aggtcggcga tcgtggccgc gtccgcgatg ccgtccgctg actgccaggt
     6421 gcgggtcgtg atcccgtgca gcgccgcgtg ggcgccaagc tcggcgcgga cgatgtgcgc
     6481 ccgcacgtcg gcgaccacct cctgccccgg tggcaccagg caccacacgc ccagcaggtt
     6541 ggccatcgaa ccggtggtgc agaacagccc tgcctcgtga ccgagcagct cagccacccg
     6601 ggcctcgaac gcgttcagcg tcgggtcctc gccgtagaca tcgtcgccca cctcagcgga
     6661 cgccatcgcg gccagcatcc ccggcgtcgg cttcgtgagc gtgtcactgc ggagatcgat
     6721 cacttcagaa atcctcggcc gagtactcct gcaccgggtt cacccggcgg atccggccgt
     6781 cggtcagccg gtcggcgatc aggaaggaca actccagcga ctggttgcgg ttcagcctcg
     6841 ggtcgcaggc ggtctcgtag cggttggcca gatcggcctc ggccagctcg tccaccccgc
     6901 ccacgcactc ggtgacgtcg tcgccggtca gctcgatgtg cacgccaccc ggccaggtgc
     6961 ccaggccctc gtgcacgtcg aagaagccgt tcagctcgtc ggccacctgg ctgaacgccc
     7021 gggtcttgta gccgagcgag gtctcgaacg tgttgccgtg catcgggtcg cagacccagg
     7081 ccaccttgcg tccggtctgc tcgaccgcct cgacgatcgg cggcagggcg tcgcgaacct
     7141 ttgccgcacc catccggctg atcaccgtca gccgtccggg ctcgtggtcg ggatccagct
     7201 tgtcgaacag tgccgtcacc tcggacggga ccgcggacgg cccgagcttg acgccgatcg
     7261 gattgcgcag gtgccgcagg tactccacgt gcgcgccgcc gagttgccgc gtccgctcac
     7321 cgatccagac catgtgcgcg gacgtgttgt acggcgtgtc cgtgcgcgag tcgattcggg
     7381 tgagggcgtg ctcgtactcc agcagcagcg cctcgtggct ggagtagaag tcgaccgtcc
     7441 ggaacgactc cttgtcgaca ccgcaggcca ccatgaacgc cagcgcgcgg ctgatctcgt
     7501 cggccaggtg ctcgtagcgc tcctccacgt tcgagttgtg cacgaagtcg gcattccagg
     7561 tgtggacggc gcgcaggtcg gcgaacccgc ccttcacgaa cgcacgcagc aggttaaggg
     7621 tcgccgcgga gtagttgtag acctccagca gccgctgcgg atcgggctgg cgcgcctccg
     7681 gctcgaacgc cagtgcgttc accgcgtcgc cgcggtaggc gggcagcgtg gtcgcaccgc
     7741 gggtctcgga gtcgcggctg cgcggcttgg cgtactggcc agcgatccgg ccgaccttga
     7801 gcacaggcac ctgcgcggcg taggtgagca ccacggccat gctgagcagc acccgcagct
     7861 tgccgcggat gttcgcagcg gtcaccgact cgaaggtctc ggcgcagtca ccgccctgca
     7921 gcaggaagga ccggccggcg gcaacgtcgg ccagcttgtc gcggagcacg tcgcactccc
     7981 cagcgaaaac cagcggcgga cgtcggcgca gttcggccag cacccgcgcg accgcgtccg
     8041 ggtcggggta gctcggctgc tgggccgcgc cgagcgcgtg gagttcagcc agcgaaggga
     8101 ttggttccaa cacgcgggtc aggatagccc gcctgagggc agctcaggcg gagtggcccc
     8161 gtagccggga cgcggcagca ccctcgcctc ggcgtccgca gccgtcagcg aaccgctctt
     8221 cagcgaggtg ccgtaggcct cgacgtaggt ctgcccggtc agttccatga tcgcgttcat
     8281 cacttcgtcg gtgacccagc gcagggtttc gcggtcgtca tgcagcccgg cgtacgcgcc
     8341 gaaatccatc ggcttgccga tcttgacctg cggcttccag atccacggaa tcccgagcct
     8401 gcggacggtt tgggtgttca gcatgcccac cggcaccacc ggcacgccgg cggccagggc
     8461 aaggcgcgcc acccccgtcc ggcccttgta gagacggccg tccggggagc gcgtgccttc
     8521 ggggtagatg cccaccatgc cgccgtcggc gagcacccgc agcaccgggc cgagaccctc
     8581 gaggctcacc cgtccaccgg agcgatccag tggcacctgg ccgaccgctt tcaggaacca
     8641 cgccacgatc ttcgagccga tcccgcggtt gccggcgaac agttccgcct tcgccgggaa
     8701 ggtcacctgc cggtgcagca tcgcgggcat cacgaaggtg tcgccggctg cgagatggtt
     8761 actggcgagg atcacgccgc cggtcttcgg caggttctcc tcgccctcga tcgtggcgcg
     8821 gaagagccag cgaacgatcg gggcgaacag cacgtttttg aacagggcgt agtacacgcg
     8881 gacagcctac gatgaccgca tgccttcaac tcctacgacg ccacgagtcg gcgactacgc
     8941 gcagccgttc agcgcggacg gcggccggat cggcgtcctg ttctgccacg ggttcaccgg
     9001 gtcgccctgg tcgctgctgg aatgggccaa gctgaccgcc gacgcaggtt acagcgtctc
     9061 gctgccccgg ctgcccggcc acggcaccac ctggcaggag ttgaacctga ccacctggcg
     9121 cgactggtac gacaccgtgg aggcggcgtt cctggaactg cgcagccgtt gtgatgtggt
     9181 gttcgtggcg ggcctgagca tgggcggggc gctcgccctg cggctggcgg aacgacaccg
     9241 cgacgaggtg gccggtctgg tgctggtgaa ccccgcggtg gtgggcatgc gccgaatggc
     9301 cgcgattccg ctgctgaagc tcttcctgcc ctctgccaag gcgatcggct ccgacctgat
     9361 cgacaccaag gtggacgagc acgcgtacga ccggacgccg ttgaaggcgg cgcactcgat
     9421 gatgaagatg tgggcggaca tccgcgcctg catggatctg gtgatctgcc cggtgctgat
     9481 cttccgctcc gccaccgatc acgtggttcc ggcagcgtcc acggcgttcg tgctcagcca
     9541 tgcatcgagt gaggacatca gcgaacgcgt gctcgagaac agctttcacg tcgccacgat
     9601 ggaccgcgac aaggagcaga tcttcaccga gtcgctggcc ttcttcgcca agcactcggc
     9661 gaagcatccc tgagctgctc agccaccatc cctgagccga cgaagctcgg cgacgtacca
     9721 gcttcgtacc tgctcgtgct cggcttcggt gaccttcgcc gcctggtgga tcgcgcgggc
     9781 gaggaagtcg tctgcttgcc ggccccgatg cttgatcacc tggtcaacgg ccagttgagc
     9841 cagcgcatag tcggtgaggg tgttcagttc gctcggtagc ttgtcgggca gggcgtggct
     9901 ggccaggtag tcgatgacca gcttgtggtt gcgggacgct gtggacgggt ccgcgcctgc
     9961 tgccaccgac tctgcgaggc cttcggtggc ccagcccagc gacttccccg acgggacgca
    10021 ggccgaggcc gtggcgacgt gcacgccctc atgcaccaga gtgctgcgca gccacgggtc
    10081 acctgcgacg aggacgaccg ggttcaccac gatttcggac gcgccgtcgg tgcaggtggt
    10141 ggccgcggac gccgcgtccg caggtgtagc ggttctggac gcgaactctg cggcatcggt
    10201 ggcgatgacc acgtcaagct ttccctgcca gcccggcctg accgcagcgg tgatttcggc
    10261 tgcgcggacg gtgcgcgcgg cctgctcgat tcgctgcgtc caggcagcgc gatcagtctc
    10321 gctcagccgg gaggcggtga gtgaaccgaa ctcggtgctg gtgctttcgg gcccttcgac
    10381 aggctcaggg atcgtggcaa tgggcggcgt gggctgacag ccgaccagcg cgaacgccac
    10441 agcggcggcg gcaagccgtg cgcggagggt cagccgatca cccggcgggc accggcccac
    10501 ggcgccgggt aggaggccag cgtctggatc accagatcgg agcgggtgtt gcgggcgtgc
    10561 acgatcttgc cgttgccgat gtacatgccg acgtggtgga tcgggtgata ccagaagatc
    10621 aggtcaccag gcttcagctc atccttcgaa acggcccggc caacgtttgc ctgcgcgcgc
    10681 gaggagtgcg gcagcccgat gccgaccgac cggtaggcgg cgagcatcag accggagcag
    10741 tcgaagccgg tcggcccgga agagccccag acgtactggc cgttcggcac cttcgaggtg
    10801 gcgtacttga tggccgcgat gatcttggcg ttgaccgtgg tctcgtccag ttcactcgcg
    10861 tcgaacgtgg accgcccacc ttcggcagcc tccaactcag cgcgctgctg gtcggtgagc
    10921 tgcagcacca gcgcctgggc tgcgtccacc ttgtcctgca ggtccttctt catcgcggcg
    10981 agcttctgct cctgctcagc cagcgctgcg acctctgcgt ccgcggtgct cgtcatgtcg
    11041 gtgaggttgg cctgctcagc ctcgtgctgg gcgagcgtgt cggtcatgtc ctgatcgacc
    11101 ttgttggccg tcgacaggcg atcgagcagg gtgtcggggt caccgctggt gaagatctgg
    11161 acggtggtgt cgacgtcgcg gttctggaac tggcgcagag cgatcactcg aacctgctcg
    11221 gcgagcgtgt ccaccagctt ctgctgccgg gagatgtcgg acttgagcga cttcagccgg
    11281 tccttgttct cttcgagggc tgccgcggcc acctcgtagt cctcgccgac ctcgctgacc
    11341 tgcttctcga gctcctcgac ctgggccttg gcgtccttga tcttggccgc gatcggctgc
    11401 gcggatgggg tcggcgtggg cgttggggtc ggtacggcgg atgcaggatc agcgaacgga
    11461 cccgctacga caacgagagt ggcagccgcc acagcgagcg cagtcctggc atgacgccct
    11521 gcctgcatta ctgcctcccc ttgcctgaac ttcgttagcc aactggtcga gcccagccga
    11581 cgtgggccgc cagctgaagc taaactgaga aaatgctaag ggacggcgcg tagcttggca
    11641 actctctcct gctcccaggt cggtggagtg ggcaccaatc gcagcggtgg caatagaccg
    11701 gaatcggcca tggcggcaag cgcgacctgc tcggtgtcgt ccagcgtgag acctgcgggc
    11761 atcggcccca acaggactga gatcacacag tccgcacaag ccgggccgcg ggctgcgcag
    11821 gtggcgcaat cgacatgcaa tacatccatg ggcacatcgt ggcggtcccc tccgacagtt
    11881 ttgtcgccta cccttcgcag atgggtgcaa gggcctggtc cgcggcgggt ttggcggtaa
    11941 tcctggctgc cggctgcact tcttcgccgt ggcaggccgt gccgcttccg gacgggctgg
    12001 aaccggcttc gttggcggcg accgccgaag gcgtcctcgt cgggggcaag gccgggactg
    12061 gtccggcact gttgcgg
//
LOCUS       DAOPRQ010000069        11653 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_4658_length_11653_cov_11.188222, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000069 DAOPRQ010000000
VERSION     DAOPRQ010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 11653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11653
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4658_length_11653_cov_11.188222"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..968)
                     /gene="pip"
                     /locus_tag="PKJ61_09330"
     CDS             complement(<1..968)
                     /gene="pip"
                     /locus_tag="PKJ61_09330"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004811668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HOC14065.1"
                     /translation="MTTDPRTEQVLYPPIEPYASGFLEVGDGQQLYWEECGNPDGKPV
                     VFVHGGPGGGCIPGYRRFFDPSRYRVILFDQRNCGRSRPHASTPDADLSQNTTWHLVA
                     DLEALREDRSVERWQVFGGSWGSALALAYAEAHPERVTELVLRGIFTLRRFELDWYYN
                     GGAGHLNPVWWRAFCAPLGDGFSGDRIAAYHDLLFDPDPAVHLPAGVAWTTWEAATSN
                     LEFSQAHVDEFSDPAFALAFARIENHYFVNAGWFREGQLIEDVGRIRHIPAVIVQGRY
                     DLPCPAVTADDLHRAWPEADYRLVLAGHAAMEPAIATELVAATDRFA"
     gene            complement(958..1392)
                     /locus_tag="PKJ61_09335"
     CDS             complement(958..1392)
                     /locus_tag="PKJ61_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5709 domain-containing protein"
                     /protein_id="HOC14066.1"
                     /translation="MTDYDEAVPEEAEQLDQLQPDETLLDRGVGDVLDEGYVTSENWS
                     QGQGFGNTPAEMRQGETLEMRMAQELPEPVPDDTPWNPDPTEPREVGHQRAGRLVDAN
                     GGIPGIDNEAESIGHDVGIDGGAASAEEAAMHIIDDYDDDDD"
     gene            1523..2227
                     /locus_tag="PKJ61_09340"
     CDS             1523..2227
                     /locus_tag="PKJ61_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HOC14067.1"
                     /translation="MSQPNPASSRPRGNYDLVLAAFVGLLLISNIGATKLIAFGPRME
                     VAGLPLLPIITDGGAILFPLTYILGDVLAEVYGLRRANRAILTGFVLAGVMSLTFLLV
                     DAAPPATDWPNQDAWHAVLGFVPRIVVASLLGFLAGQFLNAWVLVWIKNRWGSGRLWV
                     RLISSTIVGEFADTLVFCLIAFGPLGNVLGGESIPWPVLLNYIAVGWLYKVGVEVVLL
                     PVTYRVIAWVKRRESA"
     gene            complement(2307..2564)
                     /locus_tag="PKJ61_09345"
     CDS             complement(2307..2564)
                     /locus_tag="PKJ61_09345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14068.1"
                     /translation="MRHQVEGDVLVASHDAAALDELAGTRPRLAELPEFRRAVPDHDR
                     AIASAYVDLRAAGWPRVEPGSACAAFADSISALGGALPIHR"
     gene            complement(3161..4411)
                     /gene="tgt"
                     /locus_tag="PKJ61_09350"
     CDS             complement(3161..4411)
                     /gene="tgt"
                     /locus_tag="PKJ61_09350"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOC14069.1"
                     /translation="MPEGFGFTPGAVLENGLGRSGVIRTPHGEIRTPAFIAVGTKATV
                     KAVLPETVRELGAQAVLANAYHLYLQPGSDIVDAAGGLGRFMNWPGPTFTDSGGFQVM
                     SLGVGFKKVLAMDATGLANDDVIAEGKNRLAHVDDDGVTFTSHLNGDRHRFTPEVSMR
                     IQHELGADIMFAFDECTTLMNTREYQERSLARTEAWAARCLVAHAELTGERVGKPYQA
                     LFGVVQGAQFEDLRRRAARHLAELEVAGTQFDGFGIGGALEKENLGTIVGWVNSELPD
                     DKPRHLLGISEPDDLFAAVAAGADTFDCVMPSRVARNAAIYTASGRFNVNTAKSRRDF
                     GPLDPECDCYTCANYSRAYLHHLFKAKEMLGATLATIHNERFTVRLVDRIRASIDGGD
                     FLAFRTEFLGRFYGGRSQPTTTTF"
     gene            complement(4404..5204)
                     /locus_tag="PKJ61_09355"
     CDS             complement(4404..5204)
                     /locus_tag="PKJ61_09355"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013160785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HOC14070.1"
                     /translation="MTEPLFLALLDAPEVGQTVTLGGDEGRHAATVKRIRVGEVVLIS
                     DGNGAAVRGPVLTVDKSQLTVRVEAVLHEQRPTVKYVAVQALPKGDRAEQAVEMLTEL
                     GVDEIVPWQAERSVVRWAADRVERGLTRWRATAREAAKQSRRFRVPTVSVPMTTGELV
                     QRIAQTDLTVVLHESADAPLAALELPAEGEIMFIVGPEGGLTDAEVAAFTQAGARAAL
                     VADAVLRTGTAGVVALAQLHALAQRHELARHELAQPHVALPDSAATSA"
     gene            complement(5201..6370)
                     /gene="dnaJ"
                     /locus_tag="PKJ61_09360"
     CDS             complement(5201..6370)
                     /gene="dnaJ"
                     /locus_tag="PKJ61_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HOC14071.1"
                     /translation="MSSDYYEVLGVPRDATSEQIKKAYRRLAMKYHPDVADAPDAAEK
                     FKAIGEAYEVLHDQNKRAMYDRGGDPLGGGFGAGASGFGFPGGGGFDFTNLVDAMFGT
                     QTSRGPRSRTRRGQDALVRMDLTLAEAAFGITKPLRVDTAVICPRCSGTGANEGSEPV
                     TCSTCHGAGDVTQVQRSFLGEIRTSQPCPSCRGYGTVIPNPCGECSGEGRVRSNRTIN
                     VKIPAGVASGNRVHLVSQGEVGPGGGPAGDLYVELNVASHDTFKRNGDNLEMVVKVPM
                     TAAALGTQISLATLEAERDDLDADAASVLLDIPAGTQSGTRLNVPGRGVPRLRGEGRG
                     ELGVTLLVQTPTKLDDEQRELLRQFASLRDESRLEVSVAKHGKGGVLGWLKEAFS"
     gene            complement(6374..7387)
                     /gene="hrcA"
                     /locus_tag="PKJ61_09365"
     CDS             complement(6374..7387)
                     /gene="hrcA"
                     /locus_tag="PKJ61_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002519052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HOC14072.1"
                     /translation="MLDDRKLRILRAIVTDYVSMREPIGSRALVERYQLDVSPATVRN
                     DMAVLEEEGYITQPHTSAGRIPTDKGYRLFVDRLGAVKPLSSAERRAIETFMDGALDV
                     DEVVRRTVRLLAQITQQVAIVQYPVLGAATIRHVEIVALTPERGVLIVVNSSGRVEQH
                     PVELGGQSEAAVADLRGRLNACLSGLAPAQAIERTSTLLDELPADLRPLGTVVIAALL
                     ELISSDPTTRVVVGGVPNLTRFSDQYETTVRPVLEALEEQVVLLRLLGEATAAGDVSV
                     RIGHENSYAPLRSTSVVASAYGANPDAWATLGVVGPTRMDYPSTMAAVRAVARYVGRF
                     LEG"
     gene            7465..8304
                     /locus_tag="PKJ61_09370"
     CDS             7465..8304
                     /locus_tag="PKJ61_09370"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOC14073.1"
                     /translation="MVQFNVGEFTPVAEKILVAVAEPAGVNIGLVIGKSGCLVIDTGS
                     SPAQGAVIRRAAERASGLPVVGVLVTHWHYDHLFGLAGFAGVPSYGHDTVAAWLSRPE
                     CREAATGLGVEMTDLVAPSQSFSLARVIDVGGRRVEVVHFGRGHSDGDVVAIVPDAGV
                     IFAGDLLESAGPPEFGSDSYVREWPSAVDGILGLVAEETVIVPGHGAPMDRLAAFTQR
                     AEISALYGQVEHLISRGVRLPDAYAEGEWPFDEATVKSVLPRLYADFAATGLTPRTQL
                     PLL"
     gene            complement(8358..8537)
                     /locus_tag="PKJ61_09375"
     CDS             complement(8358..8537)
                     /locus_tag="PKJ61_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5302 family protein"
                     /protein_id="HOC14074.1"
                     /translation="MAEQDNDDLKAKMREALARKQHGPEGAGDTGHHGKEKGPAERGR
                     QGGPGMFRRKAGGGG"
     gene            complement(8600..9355)
                     /locus_tag="PKJ61_09380"
     CDS             complement(8600..9355)
                     /locus_tag="PKJ61_09380"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HOC14075.1"
                     /translation="MSDVLIDVAARLILPRFGSLHSADIHEKAPGDLVTVADREAEVE
                     IGRILLQDDPNALIVGEEGVFAEPGSLDQLPNARHAWVIDPVDGTRNFARGRGDFAVM
                     LAEVRDRVTTRGWIWQPVHSRMYVAERGAGVTCNGKQLPPPPPSPRSVPLGATYLPVS
                     GEDHAKVKVVRSWGSCGIDYPKLIEGEVDFLSYRSMFPWDHLAGGLMATELGGRMATD
                     AGVDYQAGVIGRRLLTAANPQLWQTARDALVGG"
     gene            complement(9488..10306)
                     /locus_tag="PKJ61_09385"
     CDS             complement(9488..10306)
                     /locus_tag="PKJ61_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015011497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3097 domain-containing protein"
                     /protein_id="HOC14076.1"
                     /translation="MRYSEDVLQPGWQRAGKPVSAETPVAVGLVVEDPTSGFCGAIVR
                     WENGLVVLEDRLGKRRSFPFGPGFWLDGKPVSLVAPKPVPRNQPTHTASGSRIGVPSA
                     AKVALESRIYVEGRHDAELVEKIWGDDLRHVGVVVEYLGGIDDLVSIVAEFQPGPGRR
                     LGVLVDHLVAGTKESRIVAGLDSAHVLVTGHPFIDIWQAVLPQRVGLDAWPQVSRRVD
                     WKTGISTALGRPVRSQADIAATWQWILGRVRDWHDLDPALPREVERLIDFVTTD"
     gene            complement(10528..11235)
                     /locus_tag="PKJ61_09390"
     CDS             complement(10528..11235)
                     /locus_tag="PKJ61_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOC14077.1"
                     /translation="MLEVKDLVVAYGRVKAVKGISFTVEQGQVVTLLGTNGAGKSTTL
                     RTISGLIPLVSGEIWFEGKRLDGIPAHKVVERGIAHSPEGRRLFRQMTVEENLKLGAY
                     SRKDADGVKRDLARVYEMFEVLGERRNEAAGLFSGGEQQMLAIGRALMSQPKLLMLDE
                     PSMGLSPIMTMRVMATIKELQSQGMTILLVEQNAQAALSLADRGYVLEVGQIMFSDSG
                     RNLLGDDRVKRTYLGVD"
     gene            complement(11228..>11653)
                     /locus_tag="PKJ61_09395"
     CDS             complement(11228..>11653)
                     /locus_tag="PKJ61_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOC14078.1"
                     /translation="ELLAFVGLTAAENVLSRNLPYGDQRRLEIARALATDPKLLLLDE
                     PTAGMNPQETRQAEDLIFRIRDLGLAILVIEHDMRFIFNLCDRVACLVRGGVLVEGPG
                     SVVQNDPRVIEAYIGTPPSEWAAEAERPAQDHGNGAVDA"
BASE COUNT         1801 a         4098 c         3828 g         1926 t
ORIGIN      
        1 gcgaaacgat cggttgcagc caccaactcg gtggcgatcg ccggctccat agctgcgtgg
       61 ccggccagca ccagacggta gtccgcttcc ggccaggctc ggtgcaggtc gtcggcggtc
      121 accgccgggc acggcaggtc gtagcgcccc tgcacgatga ccgccggaat gtgccgaatg
      181 cgtccgacgt cttcgatcag ctggccctcg cggaaccagc ccgcgttcac gaagtagtgg
      241 ttctcgattc gtgcgaacgc cagcgcgaaa gccgggtcgc tgaactcgtc cacgtgtgcc
      301 tgggagaact caagattgga ggtcgcggcc tcccacgtcg tccaggccac accagccggc
      361 aagtgcactg ccggatcagg gtcaaagagc aggtcgtggt aggcggcaat ccgatcgccg
      421 ctgaaaccgt ctccaagcgg ggcgcagaac gcgcgccacc agaccggatt gaggtgtccg
      481 gcgcccccgt tgtagtacca gtccagttcg aagcgccgca gcgtgaagat gccgcgcagc
      541 accagctcgg taacccgctc cgggtgcgcc tcggcgtagg cgagggccag tgcgctcccc
      601 cacgaaccac cgaagacctg ccagcgctcg acgctgcggt cctctcgaag ggcctccagg
      661 tcagccacca gatgccaggt ggtgttctgg ctcaggtccg cgtccggagt cgaggcgtgc
      721 ggacgcgatc gtccgcagtt gcgctggtca aacaggatca cccgataccg cgacgggtcg
      781 aagaaccggc ggtagccagg tatgcagccc ccgcccggcc caccatggac gaacaccacc
      841 ggttttccgt ccgggttgcc gcattcctcc cagtacaact gctggccgtc gccgacttcc
      901 aggaagccac tggcgtacgg ctcgatcggc ggatagagga cctgttcggt tcggggatca
      961 gtcgtcatcg tcgtcgtagt cgtcgatgat gtgcatcgcc gcttcctccg cggacgctgc
     1021 cccgccatcg atgcccacgt cgtgaccgat gctctcggct tcgttgtcga taccgggaat
     1081 cccgccgttg gcgtccacca agcgaccggc gcgctggtga cccacctcgc gcggctcagt
     1141 ggggtcgggg ttccacgggg tgtcgtcggg caccggctcc ggcaactcct gcgccatgcg
     1201 catctccagg gtctcgccct ggcgcatctc agcaggagtg ttgccaaatc cctggccctg
     1261 cgaccagttc tcgctggtca cgtagccctc gtccagcacg tcgccaaccc cacgatcgag
     1321 cagggtctcg tccggctgca gctggtcgag ctgctcggcc tcttccggga ctgcttcgtc
     1381 gtagtcggtc atgggcctag attgccacgt ccgggcccgg attgggtcgg caacgccgac
     1441 aactacgggt gtgaaccttc tcgcccgggt tgcacagttg cgagggcaat tgccggacgc
     1501 gagtgatcta ggattcgctc tcgtgtccca accgaatccg gccagcagtc gaccgcgtgg
     1561 caactacgac ttggtgctgg ccgcgttcgt cggtctgctg ctgatctcga acatcggtgc
     1621 gaccaagctg atcgccttcg ggcctcggat ggaagtggcg ggcctgccac tgctgccgat
     1681 catcaccgac ggcggcgcga tcctgttccc gctcacctac atcctcggcg atgtgcttgc
     1741 cgaggtgtac ggactgcgca gggcaaaccg cgcgatcctg accggtttcg tgctggccgg
     1801 cgtaatgtcg ctcaccttcc tgctggtgga cgcggccccg ccagccaccg actggcccaa
     1861 tcaggacgcc tggcacgccg tgctcggttt cgtgccgcgg atcgtggtgg ccagcctgct
     1921 gggcttcctt gccgggcagt tcctgaacgc ctgggtgctg gtctggatca agaatcgttg
     1981 gggcagcgga cggctctggg tgcgcctgat ctcgtcgacc atcgtcggcg agttcgccga
     2041 caccctggtc ttctgcctga tcgctttcgg ccccctgggg aacgtactgg gcggcgagtc
     2101 gatcccctgg cccgttctgc tcaactacat cgctgtcggc tggctgtaca aagtgggcgt
     2161 cgaagtggtg ttgctgccgg tcacctatcg cgtgatcgcg tgggtgaaac gcagggagtc
     2221 agcctgaccg cctgtccaac cggcggcgca cgctcagggc gggacgtcag ccgcgggtca
     2281 accggatgga ccagccgccc gtcccctcac cggtggatag gtaatgcccc cccgagagcg
     2341 ctgatcgagt cagcgaaggc cgcgcaggcc gagccgggct cgactctcgg ccacccggcg
     2401 gcccgcaggt cgacataggc ggacgcgatc gcgcggtcgt gatcaggcac cgcacgcctg
     2461 aactccggca gttcggcaag ccgcggcctc gtcccggcga gttcgtcgag tgcggcggcg
     2521 tcgtgggacg ccaccagcac gtccccttcc acttggtgcc tcaagctgcc gtactccgtg
     2581 ccggcgatga ccgtggccag cgttgcttcg gcctgacccg gatcgtcggt ggtgacccga
     2641 acgtcgtact cccggtcgaa gaggtcgccg ccgttggtcg cgctccccga tccatttctt
     2701 caaggtggcc acgccacgct cggtgtcgat caattcgatc gcagtcatcc aggttggctc
     2761 gtcaggcgcc accatgatcg cgaacccagc ccgatccccc agccagggct gcacgtcggc
     2821 ggcgaaatct gcgtcgtcgg cgcagcccag ccgcgatcca atcacctgcc gcaacaggag
     2881 tttcggctcc gggcggccgc cggcggactg cacctccggc aggtcgtgga ggaaggacca
     2941 cgcctgcaac tgctcccagg gcgcgggcgc caggtcgagc tcgaggtagg cggaaacctg
     3001 agcgggcagc acgtcagccg gacgcctgcg cctgtcgaac cagccgaacg cccagctcgc
     3061 caccgcgaca ccgaccccgg gaagcgggac aagcgtgacc ataaccgcgg aaatccagcg
     3121 ccgccggtcc agctggtcag cagcgggttg ggcagctacc tcagaaagtg gtggtggtgg
     3181 gttggctgcg gccaccgtag aagcgtccca gaaactccgt ccggaaagcg aggaagtcgc
     3241 cgccgtcaat actcgcgcga atccggtcta ccaggcgcac ggtgaatcgc tcgttgtgga
     3301 tcgtggccag cgtggcgccg agcatctcct tggccttgaa caggtggtgc aggtaggccc
     3361 gggagtagtt cgcgcaggta tagcagtcgc attcgggatc cagcgggccg aagtcacggc
     3421 ggctcttcgc cgtgttcacg ttgaaccgac cggacgcggt gtagatcgca gcgttgcggg
     3481 ccacccgcga gggcatcacg cagtcaaagg tgtccgcacc agcagccacc gccgcgaaca
     3541 ggtcatcggg ctcggagatc ccgagcaggt gccggggctt gtcgtccggc aactcggaat
     3601 tcacccagcc gacgatcgtg cccaggttct ccttctccag tgcgccaccg ataccgaagc
     3661 cgtcgaactg cgtcccagct acttccagct cagccaggtg ccgggctgcc ctgcggcgaa
     3721 ggtcctcgaa ctgcgcgcct tgcactacgc cgaacagcgc ctggtacggc tttcccaccc
     3781 gctcgccggt cagctcggcg tgcgccacca ggcaccgggc cgcccacgcc tcggtgcgcg
     3841 ccagggaacg ctcctgatac tcgcgggtat tcatcaaggt ggtgcactcg tcgaaagcga
     3901 acatgatgtc ggcgccgagt tcgtgctgga tccgcatcga gacctccggg gtgaaccggt
     3961 gccggtctcc gttcaggtgc gaggtgaacg tgaccccgtc atcgtcaacg tgcgcgagcc
     4021 ggttcttgcc ttcggcgatc acgtcgtcgt tggccagccc ggtggcgtcc atcgccagca
     4081 ccttcttgaa gccaacacca agactcatca cctggaagcc gccggagtcg gtgaaggtcg
     4141 ggccgggcca gttcatgaac ctgccgaggc cgccggccgc gtccacgatg tccgaaccgg
     4201 gctgcaggta gaggtggtag gcgttcgcca gcacggcctg tgccccgagt tcgcggacgg
     4261 tctcaggcag gacggccttg accgtcgcct tggtgcccac cgcgatgaac gcaggcgtgc
     4321 ggatctcgcc gtgcggggtg cggatgacgc cgctgcgtcc cagtccgttc tccagcactg
     4381 cgcccggagt gaacccgaag ccctcaggca ctcgtcgccg ccgagtctgg gagtgccacg
     4441 tgcggttggg ccagttcgtg tcgggccagt tcgtggcgtt gggccagcgc gtgcagttgg
     4501 gccagcgcca ccacgccggc cgtgccggtg cgcagcaccg cgtccgccac cagcgcagcc
     4561 ctggctccgg cctgggtgaa cgcggccacc tcggcgtcgg tcaacccgcc ttctgggccc
     4621 acgatgaaca tgatctcgcc ctcggcgggc agttcgagag cggccagcgg tgcgtccgcg
     4681 ctctcatgca gcacaaccgt cagatcggtc tgggcgatcc gctgcaccag ttcgccggtg
     4741 gtcatcggca ccgaaacggt cggcacccgg aatcggcggg actgcttggc cgcctcccga
     4801 gcagtcgccc gccagcgggt gagcccgcgc tcgacgcgat cggccgccca ccgcaccact
     4861 gagcgctcgg cctgccaagg cacgatctcg tccaccccga gctcggtgag catctccacg
     4921 gcctgctcag cccggtcgcc cttgggcagc gcttgtactg cgacgtactt caccgtgggt
     4981 cgttgctcgt gcagcacggc ctcaacccga acggtgagtt ggctcttgtc gacggtgagc
     5041 accggcccgc gcaccgcggc accgttcccg tcgctgatca ggaccacctc gccgacgcgg
     5101 atgcgcttca ccgtggcggc gtgccggccc tcatcaccgc cgagggttac ggtctgcccg
     5161 acctcgggcg cgtccagtag tgccaggaac aggggttcgg tcacgaaaag gcttccttga
     5221 gccagcccag caccccgccc ttgccatgct tggccaccga aacctcaaga cgcgactcgt
     5281 cccgcagcga ggcgaactgg cgcagcaact cgcgctgctc gtcgtccagc ttggtcgggg
     5341 tctgcaccaa cagggtcact ccgagctcac cgcggccctc gccacgcagc cgcggcaccc
     5401 cgcgtccggg gacgttaagt cgcgtaccgg actgggttcc cgccggaata tccagcaaca
     5461 ccgacgctgc gtcggcgtcc aggtcgtccc gctcagcctc caacgtggcc agcgagatct
     5521 gggtgccgag cgcggctgca gtcatcggca ccttgaccac catctccagg ttgtcgccgt
     5581 tgcgcttgaa ggtgtcgtgg ctagccacgt tgagttcaac atagaggtca ccggcgggcc
     5641 cgccacccgg tccgacttct ccctgcgaca ccaagtgcac ccggttcccg gacgcgacgc
     5701 ccgcagggat cttcacgttg atggttcgat tactgcggac gcggccctca ccggagcact
     5761 cgccgcaggg gttcgggatc accgtgccgt agcccctgca gctggggcag ggctggctgg
     5821 tgcggatctc gccgaggaac gagcgctgca cctgggtgac gtcgcccgcg ccgtgacagg
     5881 tcgagcaggt caccggctcg ctgccctcat tggcgccggt gccgctacac ctcgggcaga
     5941 tcaccgcggt gtcgacccgc agcggtttgg tgataccgaa agcggcctcg gccaaggtga
     6001 ggtccatgcg caccagggcg tcctggccgc gccgggtgcg cgaacgcggg ccgcggctgg
     6061 tctgggtgcc gaacatcgcg tccaccaggt tggtgaagtc gaacccaccg ccgccgggga
     6121 agccgaaacc ggatgcaccg gcgccgaacc caccgcccag cggatcgccg ccgcggtcgt
     6181 acatcgcccg cttgttctgg tcgtgcagca cctcgtacgc ctcgccgatc gctttgaact
     6241 tctcggctgc gtccggcgcg tcggccacgt cagggtggta cttcatggcc aaccggcggt
     6301 aggccttctt gatctgctcg ctggtggcgt cccggggcac cccgagcacc tcgtagtagt
     6361 cagaactcac gcatcatcct tctaggaacc gaccgacgta gcgggcaacc gcccgcaccg
     6421 cggccatcgt tgaggggtag tccatccggg tggggccgac gacgcccaac gtggcccagg
     6481 cgtccgggtt ggcaccatag gcgctggcca ccaccgaagt cgagcgcagc ggagcgtacg
     6541 agttctcgtg cccgatgcgg acggagacgt cgccggcggc cgtcgcctcg cccagcagac
     6601 gaagcagcac cacctgctcc tccagcgcct ccagcaccgg acgaaccgtg gtctcgtact
     6661 ggtcggagaa ccgggtcagg ttcggcacac cgccaacgac caccctggtg gtcgggtcgc
     6721 tggagatcag ttccagcagg gctgcgatca ccacggtgcc cagcggacgc aggtcagccg
     6781 gcaactcatc gagcagggtg gacgtgcgct cgatggcctg cgccggggcc agaccactca
     6841 agcatgcgtt caaccgcccc cgaaggtcgg ccactgccgc ctccgattgg ccgcctagct
     6901 caaccgggtg ctgctccacg cgcccggacg aattcacgac gatcaacacg ccccgctcag
     6961 gcgtgagcgc gacgatctcg acgtgccgaa tggtcgcggc gccaaggacc ggatactgca
     7021 cgatcgccac ctgctgggtg atctgggcga gcaggcggac ggtgcgccgc acgacctcgt
     7081 ccacatcgag ggcgccgtcc atgaacgtct cgatcgcccg ccgctcggcc gacgacagcg
     7141 gcttgaccgc gccgagccgg tcgacgaaca ggcggtaccc cttgtcggtg gggattcggc
     7201 cggcactggt gtggggctgg gtgatgtacc cctcctcctc cagcacagcc atgtcattgc
     7261 gcacggtggc cggggagacg tcgagctggt agcgctccac cagcgcccgc gaaccgatcg
     7321 gctcccgcat cgagacgtag tcggtgacaa tggcgcgcag gatgcgcagc ttgcgatcgt
     7381 cgagcacggc accctcccgt ccacgctggc actctcagta gtggagtgcc agtctagccc
     7441 agcattcggg ttaggcttct cggcatggtg cagttcaacg tcggcgagtt cacccccgtc
     7501 gcagaaaaga tcctggtcgc tgtggcggag cccgccggcg tgaacatcgg tctggtgatc
     7561 ggcaagtccg gctgcctggt catcgacacg ggctcctcgc ccgcccaggg tgcggtgatc
     7621 cggcgagccg ccgaacgcgc gtccgggctg ccggtggtcg gggtgctggt cacccactgg
     7681 cactacgacc atctcttcgg gctggccggg ttcgcgggcg tacccagcta cggccatgac
     7741 accgtcgccg cttggctctc gcgtccggag tgccgggaag ccgcgaccgg gctgggcgtg
     7801 gagatgaccg acctggtcgc acccagccag tcgttctcgc tcgctcgggt catcgatgtc
     7861 ggcggacgcc gcgtcgaggt cgtccacttc ggtcgcggcc atagcgacgg cgacgtggtc
     7921 gctatcgtcc cggatgcggg cgtgatcttc gccggggacc tgcttgagag cgccgggccg
     7981 cccgagttcg gatccgacag ctacgtgaga gagtggccga gcgcggtgga cggcatcctg
     8041 ggcctggttg ccgaggaaac ggtgatcgtg cccgggcatg gcgcaccgat ggaccggctt
     8101 gccgccttca cccagcgggc cgagatctca gcgctctacg gacaggtcga gcacctgatc
     8161 tcgcgtggcg tccggctgcc agatgcctac gccgaaggtg aatggccgtt cgacgaggcc
     8221 acggtgaagt ccgtgcttcc gcgtctctac gccgacttcg ccgccaccgg gctcaccccg
     8281 cggacgcagt tgccgttgct ctgagtgagc gcagcttcgg cgcgggtctc gccacgctcg
     8341 accagccgag ggttcggcta gcccccgccg ccggccttgc ggcggaacat gccggggcca
     8401 ccctgccggc cgcgttcggc cgggcccttc tccttgccgt ggtggccggt gtcgccagca
     8461 ccctccggcc catgctgctt gcgggccagc gcttcacgca tcttggcttt caggtcatcg
     8521 ttgtcttgct cggccatgtc agctcccgtc gtgtctggtg gccactctgc catctcggac
     8581 gcagggcgtc gagacggagt cagccgccga cgagggcgtc ccgcgcggtc tgccacaact
     8641 gcgggttggc cgcggtcagc agccggcgtc cgatcacgcc ggcctggtag tcgacaccgg
     8701 cgtcagtagc catccggccg cccagttcgg ttgccatcag gccgccggcg agatggtccc
     8761 aggggaacat cgagcggtag ctgaggaagt cgacctcgcc ctcgatcagc ttcgggtagt
     8821 cgatgccgca ggatccccat gacctgacca ccttcacctt ggcgtggtct tcgccggaca
     8881 ccggcagata ggtagcgcct agtgggacgc tgcgcgggct gggcggcggt ggtggcagtt
     8941 gctttccgtt gcaggtgacg cccgccccgc gctcggccac gtacatccgg gaatggacgg
     9001 gttgccagat ccagccgcgg gtggtcacac ggtcgcgaac ctcggcgagc atcaccgcga
     9061 agtcgcccct gcccctggcg aagttgcggg ttccgtccac cggatcgatg acccaggcgt
     9121 gccgggcgtt gggcaactgg tcgaggctgc cgggttcggc gaacacgccc tcttcgccga
     9181 cgatcagcgc gttgggatcg tcctgcaaca ggatccgtcc gatctcgacc tcggcctcgc
     9241 ggtcggcgac cgtcaccagg tcgccggggg ccttctcgtg gatgtcggca ctgtgcagcg
     9301 aaccgaatcg cggcaggatc aggcgagcgg ccacatcgat gagaacgtcg gacacctgct
     9361 cggtctgcac tgcgattcac ctgttctctg gcccggggaa gtaacccgcc ccaggctagc
     9421 catgcaaacg ctcacgattc gtcgtcgtcc gctctgcaaa ccaggttgcg gggccggacg
     9481 atctcgacta gtccgtggtc acgaagtcga tcagccgctc gacctcgcgg ggcagggctg
     9541 gatcgaggtc gtgccagtcg cggacgcgtc cgagaatcca ctgccaggtg gctgcgatgt
     9601 cggcctggct gcgtaccggc cggccgagcg cggtgctgat tccggtcttc cagtccaccc
     9661 gccgggacac ctgcggccaa gcgtcgagcc cgacccgttg gggcaacacg gcctgccaga
     9721 tgtcgatgaa tggatgcccg gtaaccagca cgtgcgccga atcgagaccg gccacgatcc
     9781 gggactcctt ggtgccggcg accaggtgat cgaccaggac gcccagtcgc cggccgggac
     9841 ccggctggaa ctcagcgacg atgctgacca gatcgtcgat gccgccgagg tactcgacca
     9901 ctaccccgac atggcgcaga tcgtcgcccc agatcttctc gaccagctct gcgtcgtgcc
     9961 ggccctcgac gtagatccgc gattccaggg ccactttggc agccgagggt acaccgatcc
    10021 gcgaaccgga cgcggtgtgc gtcggctggt tgcgcggcac cggcttgggc gccaccaggc
    10081 tgaccggctt gccgtccagc cagaagcccg gcccgaacgg aaagctgcgg cgcttaccga
    10141 gccggtcctc cagcaccacc agcccgttct cccagcgcac gatcgcgcca cagaagccgg
    10201 acgtggggtc ttccaccacc aggccgaccg cgaccggagt ctccgccgaa acgggcttgc
    10261 ccgcccgctg ccagccgggc tgcaacacat cctcggaata acgcacccgg tcaccttagg
    10321 cacccgactc ccgcggacgg gtcagccgcg ccgacgacac cgcgtccccg ccgaaccccc
    10381 gaccataagt tggctcctga atggaactcc cgctcccgaa tgttcgggag cgcggatccc
    10441 attcgggagc gaagttgcgt ggtcggtggg cgcgagccgc cggcgggcca gcgcggacgg
    10501 tggcccggtg ggtcagtcga cgcccggcta gtcgacgccc aggtaggtgc gcttgacccg
    10561 gtcgtcgccg agcaggttac ggccggagtc ggagaacatg atctggccga cttcgagcac
    10621 gtagccgcgg tcggcgagcg agagcgcggc ctgtgcgttc tgctccacca gcaggatcgt
    10681 catgccttgc gactgcaact ccttgatcgt cgccatcacc cgcatggtca tgatcggcga
    10741 caaccccatc gacggctcgt cgagcatcag cagcttgggc tggctcatca gcgccctgcc
    10801 gatcgccagc atctgctgct cgccgccaga gaacagcccc gcggcctcat tgcgtcgctc
    10861 ccccagcacc tcgaacatct cgtacacgcg ggccaggtcg cgcttcacgc cgtcggcgtc
    10921 cttgcgcgag taggcgccga gcttcaggtt ctcctcgacg gtcatctgcc ggaacaaccg
    10981 ccggccctcc ggtgaatgcg cgatgccgcg ttccaccacc ttgtgcgcgg gaatcccgtc
    11041 cagccgcttg ccctcgaacc agatctcacc cgacacgagg gggatcagtc cggagatcgt
    11101 ccgcagcgtg gtcgacttgc ccgcaccgtt ggtgccgagc agcgtgacca cctggccctg
    11161 ttcgaccgtg aaagagatgc ccttcaccgc cttcacccgg ccgtaggcga ccaccaggtc
    11221 cttcacctca agcatcgacg gctccattcc cgtggtcctg cgctgggcgc tcggcttccg
    11281 cggcccattc cgagggtggc gtcccgatgt aggcctcgat cacccgcggg tcgttctgca
    11341 ccacgctgcc cgggccttcc accagcaccc cgccgcgaac caggcacgcg acccggtcgc
    11401 aaaggttgaa gatgaaccgc atgtcgtgct cgatcaccag gatcgccagg ccgaggtcac
    11461 ggatccggaa gatcaggtcc tcggcctgcc gggtctcctg cgggttcatg ccggcggtgg
    11521 gttcgtcgag cagcagcagc ttcggatcgg tggccagcgc gcgggcgatc tccagccggc
    11581 gctggtcgcc gtagggcagg ttgcgcgaca gcacgttctc cgccgcggtc aggcccacga
    11641 aggccagcag ttc
//
LOCUS       DAOPRQ010000070        11546 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_4698_length_11546_cov_14.089548, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000070 DAOPRQ010000000
VERSION     DAOPRQ010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 11546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11546
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4698_length_11546_cov_14.089548"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="PKJ61_09400"
     CDS             complement(<1..353)
                     /locus_tag="PKJ61_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14079.1"
                     /translation="MTQPQPQPVAYGCYRHPDRPTYIGCQRCGRPICGDCMISAAVGF
                     QCPECVSAGAKQTRQHELPYGGTRVANTRVTTITLIGINVAVWVLYLLTGGSASRILA
                     NLLLLPSGQCTLTDQP"
     gene            415..1506
                     /locus_tag="PKJ61_09405"
     CDS             415..1506
                     /locus_tag="PKJ61_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOC14080.1"
                     /translation="MAGKRVALVLGSGGARGYAHIGAIQVLAERGYEVVAIAGTSMGA
                     LVGGLLAAGKLDEYTDWVTSLSQRDVFRLLDPAILAPGAIRAERVLARVSEILDGAMI
                     EDLPIPFTAVATDLGARREVWFSSGPVDVAIRASIAIPGVITPVMVNGRLLVDGGVLN
                     PVPVEPVIGADADFTLAVLLGGARDHALSVAPTKVSSAQRPPAEWLDRFLKGAAGVWE
                     NDVLAWITSRFGHTEDASEDSAAPSGPPKVVPATASTPEQPVGPGYEPAPPGIGLTDV
                     AAMALDTMGSLITRYRLAALPPDVTVTVPVDAAKVLDFHRAEELIELGRELTASALDE
                     AFPEDAETAAPAAQIESVAEIAAAQVAPG"
     gene            complement(1704..2414)
                     /locus_tag="PKJ61_09410"
     CDS             complement(1704..2414)
                     /locus_tag="PKJ61_09410"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HOC14081.1"
                     /translation="MTDFFEIFNPGLRYTREQQDAEKLLVVRTDQGGTGPRPLDLDSG
                     AVVLRLPGSSTYPAAMETPEVPPTDDKDWTFVLERPCLECGFASGEVDVHELPELVRA
                     ATAPWREVLARPDVAVRPDPVVWSPLEYACHVRDVLRLFAERATLIRSEDNPVFANWD
                     QDATAIAKRYRASDPAEVAAAIAAAAEANAAEWAAVTEDEWGRPGTRSNGSAFTLATL
                     GQYFLHDLRHHLHDVGAA"
     gene            complement(2422..2946)
                     /locus_tag="PKJ61_09415"
     CDS             complement(2422..2946)
                     /locus_tag="PKJ61_09415"
                     /inference="COORDINATES: protein motif:HMM:NF021482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2199 domain-containing protein"
                     /protein_id="HOC14082.1"
                     /translation="MVPVIDGPDTPCSVCHDVHRDLAKVMGTTAPDDWLAISDGERLE
                     AELTPDVCILSDHGTTRHFVRGHIQLPIEDVPDVFVWSVWVELDEASMAAIAQRWSDP
                     NRASMPPFAGRLATELPYEKPTKGLQVSVHTRDPGMVPLLMLSASSGHVLTVEQVTGI
                     PLHRVAEFAGLVQR"
     gene            3094..3498
                     /locus_tag="PKJ61_09420"
     CDS             3094..3498
                     /locus_tag="PKJ61_09420"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HOC14083.1"
                     /translation="MLAAAADAGHADLGKMESFISAIDEYLQGFDPSAREQLKRVREI
                     IREVLPQASEKIAYGIPTFVVDGRNLVHFGGWTDHLSIYPVPAGDAAFQAAITGHRAG
                     KGTLRFPLSEPLPEQLIRAQVLFLLAERGDRG"
     gene            complement(3548..4195)
                     /locus_tag="PKJ61_09425"
     CDS             complement(3548..4195)
                     /locus_tag="PKJ61_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14084.1"
                     /translation="MKLLTTLIATLAVLLAGCATPGNNWTPGPIVTIDAAPSETASAG
                     GGTPSATPSASSTEVGPPTVPDPSDAINVASYGRAHFASPSGRIWCAVEDGWALCHFP
                     KGMKMSKVPESSEVCPDDGLDVTGISVDAATSYFCSGDPAAFPKVGDASTKWWKSSGF
                     PSVKYDGTKFAVLPYGKKLIFDHFICASEKAGVTCANTDTGAGFRVALAGVTLIK"
     gene            4348..5196
                     /locus_tag="PKJ61_09430"
     CDS             4348..5196
                     /locus_tag="PKJ61_09430"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOC14085.1"
                     /translation="MGDNLSMTPSGDRALVLGSGGATGIAWQAGVLAGLADSGVELTP
                     ATVIGTSAGALVAARLTTGWTPERLFAVASAESLAPGRVGLGAVLALGAAQVYPSRRH
                     ALAWLGRRAERAWSADAETRWVARLVPDLAGAEWPDGLVIVATDAASGRPAFFSHSQP
                     ADLARAVAASCAIPGVFPAVRIDGRLHLDGGLRSPANLDVVASATSVIALAPLGGSVR
                     AYRRPAMQAKALGRRAAVALLRPDERSRKAMGSDLLASHRNSAAAAAGREQGRREGER
                     LGGWPA"
     gene            5407..8793
                     /locus_tag="PKJ61_09435"
     CDS             5407..8793
                     /locus_tag="PKJ61_09435"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HOC14086.1"
                     /translation="MFNKMLVANRGEIAVRAFRAATELGIGTVAVFPYEDRHAEYRLK
                     ADESYEIGERGHPVRAYLDVPGIIKAAQDAGADAIYPGYGFLSENPDLAQACEEAGIK
                     FIGPSHEVLELTGNKARAISAAIAAGLPTLRGSEPSNDVDTLVAAADSIGFPLFVKAV
                     SGGGGRGMRRVEEADKLRAALAEAMREAESAFGDSRMYLEQAVIRPRHIEVQILADAT
                     GEVIHLFERDCSVQRRHQKVVEIAPAPNLDPELRRRICSDAVRFARHINYLNAGTVEF
                     LLGEDGRYVFIEMNPRIQVEHTVTEEVTNVDLVSSQIRIAAGETLVGMGLTQADIQVR
                     GTALQCRVTTEDPANGFRPDTGIISVYRTPGGAGIRLDGGVVFAGATVAAHFDSMLVK
                     LTCRGTDLRSAARRAFRALSEFRVRGVSTNIPFLEAVISDPDFLDGHTTTSFIDDRPH
                     LLHYHLPADRGTKLLTYLAEVSVNKPNGDPRTLLRPRKKLPVLPPAVASAPPPAGSRQ
                     RLLEVGPAQWAAELRAQSAVAVTDTTFRDAHQSLLATRVRTRDLLHVAPVIARTLPQL
                     FSVEAWGGATYDVALRFLKEDPWDRLDALHAAMPNLPLQMLLRGRNTVGYTPYPLAVT
                     KAFVAEAARSGLDVFRIFDALNNVDQIRPAIEAVLETDHAVAEAALCYTGNMTSPGES
                     LYTLDYYLRLAEELVSAGAHILAIKDMAGLLRAPAAAALVTALRSNFDLPVHLHTHDT
                     AGGQLGTLLAAIDAGVDAVDVAAASMAGTTSQVSMSALVAALEGTDRDTGLSLAAVTA
                     LEPYFEGVRKLYAPFESGLPGPTGRVYVHEIPGGQLSNLRQQAIALGLGDRFEAIEEM
                     YAAANRILGNIVKVTPSSKVVGDLALALVGQKIDPSDFEANPERYDIPDSVIGFLNGD
                     LGDPPGGWPEPFRTKALAGRVTKPVVTELSDADASDLAEKPRRTLNRLLFPGPLRDYE
                     EAVVAYSELSVVPTREFLHGLRQGQEIEVSLERGKSLLLGISAIGEPDDRGVRQVVCT
                     VNGQMRVVSVKDDSVTSTVAMAERADAKSPGQIPAPFAGVVTLIVEAGDQVAAGQQVA
                     TIEAMKMEAAITTPVAGTVQRLAIGHAQAVEGGDLLAVIA"
     gene            9033..9962
                     /gene="folP"
                     /locus_tag="PKJ61_09440"
     CDS             9033..9962
                     /gene="folP"
                     /locus_tag="PKJ61_09440"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015069622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOC14087.1"
                     /translation="MDQPFASSVPVSTVPLVLRGRRFDAARPAVMGIMNRTPDSFYAA
                     ARFTDLEPALVALDRMLVEGADVIDVGGVRAGQEGEVVDAAVEIERVRPFLLAARERH
                     PEVLLSLDTWRSVVALACADARVDLVNDTWAGADPELVHVAVEIGAGYVVSHTGGLPP
                     RTDPVSVSYGDDPDAVVADVLRTLASGAERAVAAGVPAERVLVDPTLDFGKTTRNSLR
                     VLRATAAVTGLGYPVLQALSRKDFVGETLDLPVDDRLYGTLAATAVAGWLGTTMFRAH
                     DVLATRQVVDMVASIRGDRPPAVALRGEPPRSR"
     gene            10186..10818
                     /gene="pdxH"
                     /locus_tag="PKJ61_09445"
     CDS             10186..10818
                     /gene="pdxH"
                     /locus_tag="PKJ61_09445"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016668102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HOC14088.1"
                     /translation="MELKDMRLSYEKGSLDEADAGSAPLQLFQRWLNDAIRANVPEPN
                     AMTLATVGADGRPSTRIVLIKSADASGLVFFTNYDSRKGQELATNPQAALQFHWVQLE
                     RVVRIEGVVERTSAEESDAYFETRPLDSRIGAWASPQSQVIANRGVLLANAAKASAKF
                     GLKPPRPDHWGGYRLVPDVWEFWQGRKSRLHDRVRFRLEGDAWLKERLAP"
     gene            complement(10831..11406)
                     /locus_tag="PKJ61_09450"
     CDS             complement(10831..11406)
                     /locus_tag="PKJ61_09450"
                     /inference="COORDINATES: protein motif:HMM:NF025675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcxB family protein"
                     /protein_id="HOC14089.1"
                     /translation="MLEFSLTEADLAAFAAFRAQESGEEAKRIERIRLITPWVTGTIA
                     YLVVSLAFVIPMILGRQWALAGVGELVAIAVLAVLAVWQSRHLDPVLGRLLPRRYQAK
                     AQQALARTGSERKLWLDSDGLSVAGAGRTNHIAWDGITRIADTDEHWFIYTGADAAHV
                     LPKRLGAPAAELVAAISAEGCPPNTVDNPPH"
BASE COUNT         1746 a         3860 c         4084 g         1856 t
ORIGIN      
        1 ggctggtcgg tcagcgtgca ctgcccggac ggaagcagca acaggttggc caggatccgc
       61 gaggccgacc cgccggtgag caggtacagc acccagacgg cgacgttaat tccgatcagc
      121 gtgatggtgg tcacccgggt gttggcgacc cgcgtcccgc cgtacggcag ctcgtgctgc
      181 cgggtctgct tggctccggc cgagacacat tcggggcact ggaaaccgac cgccgcgctg
      241 atcatgcaat ccccgcagat cgggcgtccg caccgctggc agccgatgta ggtggggcga
      301 tccggatgcc ggtagcagcc gtaggctacc ggctgcggct gcggttgcgt catggccaaa
      361 ccctaccggc ccacccgagc cgtgacgcgt ccgccggccg cccataatgg acgcatggct
      421 ggcaagcggg tggcactggt tctgggctcg ggcggagcac gcggctacgc ccacatcggg
      481 gctatccagg tgctggccga gcgtggctat gaggtcgttg cgatcgccgg cacgtcgatg
      541 ggcgcgttgg tcggcgggct gctggctgct ggcaagctcg acgagtacac cgactgggtc
      601 acttcgctgt cgcagcgcga cgtctttcgc ttgctcgatc cggcgatcct tgctcctggc
      661 gcgatccggg ccgaacgggt gctcgcgcga gtttcggaga tcctggacgg cgcgatgatc
      721 gaagacctgc cgattccgtt caccgccgtg gccaccgatc tcggcgcacg ccgcgaggtg
      781 tggttctcct ccgggccggt ggacgtggcg atccgggctt cgatcgcgat cccgggcgtg
      841 atcacgccgg tgatggtcaa cggacggctg ctggtcgacg gcggcgtgct gaacccggtg
      901 ccggtcgagc cggtgatcgg tgcggacgcc gacttcacct tggcggtgct gctgggcgga
      961 gcccgtgacc acgcgctctc ggtggcaccg accaaggtga gttccgcgca gcgtccaccg
     1021 gccgagtggc tggaccggtt cctgaaaggg gctgccgggg tgtgggagaa cgacgtgctt
     1081 gcctggatca cgtccagatt cggccacacc gaggacgcgt ccgaggactc cgccgcgccg
     1141 agcggtccgc cgaaggtggt cccggctacc gcttcgacgc cagagcaacc ggtcggtccc
     1201 ggctacgagc cggcgccgcc cggcattgga ctgaccgacg tggcggcgat ggccctggac
     1261 acgatgggtt cgctgatcac ccgctaccgc ctcgcggccc tcccgcccga tgtcaccgtt
     1321 acggtgccgg tggacgcggc caaggtcctc gatttccacc gggccgagga actgatcgaa
     1381 ctcggccggg aactgaccgc gtccgctctg gacgaggcgt tcccggagga cgctgagacc
     1441 gcagcgcccg ccgcccagat cgagtccgtc gcggagatcg ctgctgccca ggtggctccg
     1501 ggctgaggag cgtcccagga ccggtctgga cgggttttgg gagccgattc ggccggcgcc
     1561 attcaagaaa cgggacaatt ccggtccgcg tggacgggtt ctcgggctaa ccggtagaaa
     1621 ccgccgacct cgtcgcgccg atagcgtcag ctttgtccca ttcgtgcggt gatgccggcc
     1681 gaatcgttgg gtcgtggccg aactcaggca gcgcccacgt cgtgcaggtg gtgacgcagg
     1741 tcgtgcagga agtactgacc cagggtcgcc agggtgaagg cagagccgtt gctgcgtgtc
     1801 cccggccggc cccactcgtc ttcggtcacc gcggcccact cggcagcgtt ggcctcggcg
     1861 gcggctgcga tcgccgcggc cacctcggca ggatcggacg ctcggtagcg cttggcgatg
     1921 gcggtcgcgt cctggtccca gttggcgaag accgggttgt cctcggaccg gatcagtgtg
     1981 gcccgctcgg cgaacagccg cagcacgtcc cgcacgtggc aggcgtactc cagcggcgac
     2041 caaaccaccg gatcgggccg cacagccacg tccggtcggg ccagcacctc ccgccagggt
     2101 gctgtcgcgg cgcgcaccag ctcaggcaac tcgtgcacat ccacctcgcc cgacgcgaag
     2161 ccgcactcca ggcacggccg ctccaacacg aatgtccagt ccttgtcgtc ggtcggcggt
     2221 acctctggtg tctccatcgc cgcaggatag gtgctggacc cgggcaggcg cagcaccaca
     2281 gctccggagt cgagatcgag cggacgtggt cccgtcccgc cctgatcggt gcgcacgacc
     2341 agcagtttct ccgcatcctg ctgctcgcgg gtgtagcgca acccggggtt gaagatctcg
     2401 aagaagtcgg tcatgcaggg ttcaacgctg gacgagcccg gcaaactccg cgacccggtg
     2461 cagcgggatg ccggtcacct gctcaaccgt gagcacatga cccgaactgg ccgacagcat
     2521 cagcagcggc accatgcccg gatctcgggt gtgcacactg acctgcagcc ctttcgtcgg
     2581 cttctcgtag ggcagctcgg tggcgagcct gccggcgaac ggcggcatcg aggcccggtt
     2641 cggatccgac cagcgctggg caatcgccgc catcgacgcc tcgtccagtt ccacccagac
     2701 cgaccagacg aagacgtcgg gcacgtcttc gatcggcaac tggatgtggc cgcggacgaa
     2761 atgcctagtg gtgccgtggt cggaaaggat gcaaacgtcg ggggtcagct cggcctcaag
     2821 tcgctcgccg tcgctgatcg ccagccaatc gtccggcgca gtggtgccca tcactttcgc
     2881 caagtcgcgg tgaacgtcgt ggcagacact gcacggcgtg tcagggccat cgatcaccgg
     2941 aaccatgaac tgaatctttc cgcagccggg cgccccgcgt ccggtgaact gcggatttgc
     3001 cctgaaatgg ttttcggcgc ggcttgagtt cgcgtcggca ctcgcggagc cgtcgcccga
     3061 tccgctcgcc aggaagcgcc ggctcggccc cttatgctgg ccgcagcagc ggacgctggg
     3121 cacgccgatc tggggaagat ggagagcttc atttcagcta tcgatgaata tctgcagggc
     3181 ttcgacccgt ccgcgcgcga acagctcaag cgggtccggg agatcatccg cgaggtcctg
     3241 ccccaagcga gcgagaagat cgcctacggc atccccacct tcgtggtgga cggccggaac
     3301 ctggtccatt tcggcgggtg gacggaccac ctgtcgatct acccggtgcc ggcgggcgac
     3361 gcggcattcc aggccgcgat caccggccac cgggccggca agggcaccct ccggttcccg
     3421 ctgtccgagc cgctgccaga gcagctgatc cgcgcgcagg tgctgtttct gcttgccgaa
     3481 cgcggagatc gcggctgaag ctcggtccgt ggccggccgg ttcgggcgtg gtcccgggcg
     3541 gctgagctca cttgatcagg gtgactccgg ccagggcgac ccggaagccc gcgccggtgt
     3601 cggtgttggc gcaggtgacg ccggccttct cgctcgcgca gatgaaatgg tcgaagatca
     3661 gcttcttgcc gtacggcagc accgcgaact tggtgccgtc gtacttgacc gatgggaagc
     3721 cgctactctt ccaccacttg gtactggcgt cgcccacctt tgggaacgcg gctgggtcgc
     3781 cgctgcagaa atagctggtg gccgcgtcca ccgagatgcc ggtgacgtcc aacccatcgt
     3841 ccgggcacac ttcggaagac tcgggcacct tgctcatctt catgcccttc gggaagtggc
     3901 acagtgccca gccgtcctcg accgcgcacc agatccgtcc gctcgggctg gcgaagtggg
     3961 cgcggccgta gctggccacg ttgatcgcgt cggacgggtc agggacggtc ggcggaccta
     4021 cttcggtaga gctggccgac ggcgttgcgg acggggtccc cccgcccgcg gacgcggttt
     4081 cggatggggc cgcgtcgatc gtgacgatcg gaccgggcgt ccagttgttt ccgggggtcg
     4141 cgcagccggc caacagcacg gccaaggtgg cgatcagtgt ggtgagcagc ttcatttcgt
     4201 tctctggcgg gacggtttgg gccagtctgc ccgtgcaacc gggcgacttg gctgagagcc
     4261 gaagaagaat tgcccagagg gttctttcag cttcgggtga tatttggcgg cctgccggac
     4321 ggacggctcg aagcatggct cgggctggtg ggagacaatc tgagcatgac cccgtccggc
     4381 gaccgcgcac tggtgctggg cagcggcggc gccactggca tcgcctggca ggccggagtg
     4441 ctggcggggc tggccgactc aggggtcgag ctaaccccgg caaccgtgat cggcacctcg
     4501 gccggtgcgc tggtggcggc gcggctgacc accggctgga cgccggagcg gctgttcgcc
     4561 gtagccagcg cggagtccct ggctcccgga cgcgtcggct tgggtgcggt gctggccttg
     4621 ggggcggcac aggtctaccc gagccggcgg cacgcgctcg cgtggctggg tcgacgagct
     4681 gagcgcgcct ggtctgccga cgccgagacc cggtgggtcg cgcggttggt gcctgatctg
     4741 gccggcgccg aatggccgga cgggctggtg atcgtggcca cggacgcagc gtccggtcga
     4801 ccggcgttct tctcccactc acagcccgcc gacctggccc gcgcggtggc cgcaagttgc
     4861 gccatcccag gcgtgttccc ggcggtgcgg atcgacggcc ggctgcactt ggacggtggg
     4921 ctgcggtcgc cggcgaacct ggacgtggtc gcgtccgcca cctcggtgat cgctctggcg
     4981 cccctgggcg ggtcggtgcg cgcgtaccgg cgtccggcga tgcaggccaa ggcgctcgga
     5041 cggcgcgccg cggtcgcgct gctgcgcccg gatgagcgca gccggaaggc catgggcagc
     5101 gacttgttgg ccagtcatcg caactccgct gccgctgccg ccggacggga gcagggtcgc
     5161 cgagagggtg agcgcctggg tgggtggccg gcctgagcct gcccaacggc gagggcacgt
     5221 cccctgcgtt cggcaggtcg agcctgtcga ggccacctgc gtcgccgatc tcgacccgct
     5281 cgatcaaccc gggaagcgcc ataatgggag gggaaatgtt gcgcaactga gcagcgcccg
     5341 gcgggcaggg ggtgcccaac cacgctgcag ccccaataga gtggctgacc cgctacagga
     5401 ggccaaatgt tcaacaagat gctggtcgcg aaccgcggcg agatcgccgt ccgcgccttt
     5461 cgcgcagcca ccgagctggg cattggcacc gtcgccgtgt tcccctacga ggatcggcac
     5521 gccgagtacc ggctgaaggc ggacgagtcc tacgagatcg gcgagcgcgg ccaccccgtc
     5581 cgcgcgtacc tcgacgtgcc cggcatcatc aaggccgccc aggacgccgg cgcggacgcg
     5641 atctaccccg gttacggctt cctctccgag aaccccgacc tggcccaggc ctgtgaagag
     5701 gccgggatca agttcatcgg tccttcgcac gaggtgctgg agttgaccgg gaacaaggcc
     5761 cgcgcgatct cggcggccat agcggccggc ctgccgacgc tgcgcggctc ggagccgagc
     5821 aacgacgtcg acaccctggt ggcggccgcc gattcgatcg gcttcccgct gttcgtgaag
     5881 gccgtctccg gcggcggcgg acgcggcatg cgccgggttg aggaggccga caagctccgc
     5941 gctgcgctgg ctgaggcgat gcgcgaggcc gagtcggctt tcggcgactc ccggatgtac
     6001 ctggagcagg cggtgatccg tccgcggcac atcgaggtgc agatcctggc cgatgccacc
     6061 ggtgaggtga tccacctgtt cgagcgggac tgctcggtgc agcgtcgaca tcagaaggtt
     6121 gtcgagatcg cccccgcgcc gaacctcgac ccggagttgc gtcgccggat ctgctcggac
     6181 gcggtgcgct tcgcccggca catcaactac ctcaacgccg gcacggttga gttcctgctc
     6241 ggcgaggacg gccgctacgt cttcatcgag atgaacccgc ggatccaggt ggagcacacc
     6301 gtcaccgaag aggtgacgaa cgtcgacctg gtgtcttcgc agatccgcat cgcggctggc
     6361 gagaccctgg tgggcatggg actgacccag gccgacatcc aggtccgcgg caccgcactg
     6421 caatgccggg tcacgaccga ggatccggcg aacggcttcc gtcccgacac cggcatcatc
     6481 tcggtctatc ggacgccggg cggcgcggga atccgattgg acggcggcgt cgtgttcgcc
     6541 ggggcgacgg tcgccgcgca cttcgactcg atgctggtca agctgacctg ccggggcacc
     6601 gacctgcgtt cggccgcgcg gcgggcgttc cgggcgttga gcgagttccg cgtccgcggg
     6661 gtgtcgacca acatcccgtt cctggaagcg gtgatctccg accccgactt cctcgatggc
     6721 cacacgacta cgtcgttcat cgacgaccgt ccgcatctgc tgcactacca cctccccgcc
     6781 gaccggggca cgaagctgct cacctacctg gctgaggtca gcgtcaacaa gcccaacggc
     6841 gacccgcgga cgttgctgcg tccccggaag aagctgccgg tgctgccacc cgcggtcgcg
     6901 tccgcaccgc cgccggccgg ctcgcggcag cgactgctcg aggtcggtcc ggcccagtgg
     6961 gcagccgaac tgcgcgcgca gagcgcggtg gcggtcaccg acaccacttt ccgggatgcg
     7021 caccagtcgc tgttggcgac ccgcgtccgc acccgcgacc tgctgcacgt cgcacctgtg
     7081 atcgcgcgga ccctgccgca gttgttcagc gttgaggcct ggggcggcgc gacttacgac
     7141 gtggcgctgc ggttcctcaa ggaggacccc tgggatcggc tggacgcgct gcacgcggcg
     7201 atgccgaacc tgccactcca gatgctgttg cgcggccgga acacggtcgg ctacaccccg
     7261 tacccgctgg cggtgacgaa ggcgttcgtc gccgaggcgg cccgctcggg gctggatgtc
     7321 ttccggatct tcgacgccct gaacaatgtc gaccagatcc ggccggcgat cgaggcggtg
     7381 ctggagaccg accacgcggt ggccgaggcc gcgctctgct acaccggcaa catgacctcg
     7441 ccgggcgagt cgctgtacac gctcgactac tacctgcggc tggccgaaga actggtttcc
     7501 gctggcgcgc acatcctggc gatcaaggac atggccggtc tgctgcgggc accggccgcg
     7561 gctgcgctgg tcaccgccct gcgtagcaac ttcgacctgc cggtgcatct gcacacccac
     7621 gacaccgccg gtggtcagct gggcacgctg ctggccgcca ttgatgcggg cgtggacgcg
     7681 gtggacgtcg cggccgcgtc catggctggg acgacctcgc aggtgtcgat gtcggcgctg
     7741 gtggcggcgc tggaaggcac cgatcgggac accgggctct cgctggccgc ggtgaccgcg
     7801 ctggagccgt acttcgaggg tgttcggaag ctctacgcgc cgttcgagtc gggccttccc
     7861 gggccgaccg gacgggtcta cgtgcacgag attccgggtg gacagctgtc gaaccttcgc
     7921 cagcaggcga tcgcgctcgg cctgggcgac cggttcgagg cgatcgagga gatgtacgcc
     7981 gcggcgaacc ggatcctggg caacatcgtg aaggtgaccc cgtcctcgaa ggtggtcggc
     8041 gacctggcgt tggcgctggt cgggcagaag atcgacccgt ccgatttcga ggcgaacccg
     8101 gagaggtacg acattccgga ttcagtgatc ggcttcctca acggcgacct gggtgatccg
     8161 ccgggcggct ggccggagcc gttccgcacc aaggcgctgg ccgggcgggt caccaagccg
     8221 gtggtcaccg aactgtcgga cgcggacgcg tccgatctgg ccgagaagcc gcggcgcacc
     8281 ctgaaccggc tgctgttccc cggcccgctg cgcgactacg aggaagcggt ggtggcgtac
     8341 tcggagctgt cggtggtgcc gacccgcgag ttcctgcacg gtctgcgcca gggccaggag
     8401 atcgaggtct cgctggaacg cggcaagtcg ttgctactgg gcatctcggc gatcggcgag
     8461 ccggacgacc gtggcgtgcg ccaggtggtc tgcacggtta acggccagat gcgggtggtg
     8521 tcggtgaagg acgactcggt gacgtccacg gtcgcgatgg ccgaacgggc ggacgcgaag
     8581 tcgccgggcc agatccccgc cccattcgcg ggcgtggtga ccctgatcgt cgaagccggc
     8641 gatcaggtgg ccgccggcca gcaggtggcg acgatcgaag ccatgaagat ggaggccgcg
     8701 atcaccaccc cggtcgccgg taccgtccag cgcctagcga tcggtcacgc ccaagccgtg
     8761 gaaggcggcg acctcctggc agtcatcgcc tgaggtgcca gccccactcc atttcggcga
     8821 ccacggcatc tcgagcctct tggccaggcc ttggtgaaga ccttccagac agccacgatc
     8881 atcagcacgc tcagcccgat cgcgaccagg atgaccggca gggagaggac agcgtttgtg
     8941 ttgtcggaat acgtgtttct cctggggttc ggggacgcag catagcgatt cggttcgcgg
     9001 cagggctgga cggctgcggc acagtaggag ctatggatca gcccttcgcc agcagtgtgc
     9061 ctgtgtccac cgtcccgctg gtcttgcgcg gacgccgctt cgatgcggcg cggccagcgg
     9121 tgatgggcat catgaaccgc acgccggact cgttctacgc cgccgctcga ttcaccgacc
     9181 tggaaccggc gctggtcgcg ctcgaccgga tgctcgtcga gggtgcggac gtgatcgacg
     9241 tcggcggggt ccgtgccggg caggaaggcg aagtggtgga cgcggccgta gagatcgagc
     9301 gggtgcgtcc gttcctgctg gcggcccgcg aacggcaccc ggaagtgctg ctcagcctcg
     9361 acacctggcg ctcagttgtg gcgttggctt gcgcggacgc ccgggtagac ctggtcaacg
     9421 acacctgggc gggcgccgat ccagagctgg tgcacgttgc cgtcgaaatc ggtgctggct
     9481 acgtggtttc acacaccggc ggactgccgc cgcgcaccga cccggtgtcg gtcagctatg
     9541 gcgacgaccc tgacgccgtg gtcgcggacg tgctgcgcac cttggcgtcc ggggccgaga
     9601 gggcggtggc agcgggggtg ccggccgagc gtgtgctggt ggacccgacc ctcgacttcg
     9661 gcaagaccac ccgcaactcg ttgcgcgtgc tgcgggccac cgccgcggtt accggtcttg
     9721 gctacccggt gctgcaggcc ttgtcccgca aggatttcgt gggcgagacc ctcgatctgc
     9781 cggtcgacga tcgactgtac gggacgctgg cggccaccgc cgtcgcgggt tggctgggca
     9841 cgacgatgtt ccgtgcccac gacgtgctgg ccacgcggca ggtggtcgac atggtcgcgt
     9901 ccattcgtgg ggaccggccg ccggcagtgg cgctgcgcgg cgagccgcca agatcacgct
     9961 gagctggacg agccggcgca caccccatcc aactcgggtt tggcaggctt gaccccggcc
    10021 gcctcgaaca cgtcctcgac tgtctcgatg agcttcgcca gcgggtcata gtgcctctgt
    10081 agtcgcccat ggctcgccgt catcgcgccg ctccagccga agcggagcga cgttcagcgc
    10141 actctgggac ggcctcgtcc gagcccgcgg gcataagctg ccggggtgga actgaaagac
    10201 atgcggctca gttacgagaa gggcagcctc gacgaggcgg acgcaggaag cgcgccgctg
    10261 cagctctttc agcgctggct caacgatgca atccgggcca acgtgcccga gccgaacgcg
    10321 atgaccctgg ccaccgtggg ggcggacggg cgtccgtcca cgcgaatcgt gctgatcaag
    10381 agcgcggacg cttccgggct ggtcttcttc accaactacg actcgcgtaa gggccaggag
    10441 ttggcgacca acccgcaggc cgcgctgcag ttccactggg ttcaactcga acgggtcgtc
    10501 cggatcgagg gcgtggtcga gcgcacgtcc gccgaagaat cggacgcgta cttcgagacc
    10561 cgtccgctgg attcgcggat cggtgcctgg gcctcaccgc agagccaggt gatcgccaat
    10621 cggggggtgc tgttggcgaa cgcggcgaag gcatccgcga agttcggact gaaaccgcca
    10681 cgcccagatc attggggcgg ctatcggctg gtgcccgatg tctgggagtt ctggcaggga
    10741 cgcaagagcc ggctgcacga tcgcgtgcgt ttccggctcg agggggatgc ctggttgaag
    10801 gagcgcctgg cgccgtagcc gccagtagaa tcagtgcgga gggttgtcca ccgtgttcgg
    10861 tggacaaccc tccgcactga tcgcggcgac cagctccgcg gcgggcgcac ccaggcgctt
    10921 gggaagtaca tgggctgcgt ccgcgccggt atagatgaac cagtgctcgt cggtatcggc
    10981 gatccgggtg ataccgtccc aggcgatgtg gttggtgcga cccgcgccag cgacactcaa
    11041 cccgtccgag tccagccaga gcttgcgttc gctccccgtc cgcgccagcg cctgttgggc
    11101 cttggcctgg tagcggcggg gcagcagccg gccgagtacc gggtccaggt gccgcgactg
    11161 ccagacggcc agcacggcca gcacggcaat cgccaccaac tcgcccacgc cggccagcgc
    11221 ccactgcctg cccaggatca tcgggatcac gaaagccagc gagaccacca gatacgcgat
    11281 ggttccggtc acccacggcg tgatcaggcg gattcgctcg atgcgcttgg cctcctcgcc
    11341 ggattcctgc gcacggaatg cggcgaacgc ggccaggtcg gcttcggtga ggctgaattc
    11401 gagcacgcag cgagggtagc gggtctcgag gcggacggag ttcgcgaccc aacgcctggc
    11461 tcaaggatcg tgtgcgcggc tgagagttca gctagggcgt gtcgttgcgc aacacgccct
    11521 agattccggg gacgccgagt tccctc
//
LOCUS       DAOPRQ010000071        11355 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_4805_length_11355_cov_10.822212, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000071 DAOPRQ010000000
VERSION     DAOPRQ010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 11355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11355
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4805_length_11355_cov_10.822212"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="PKJ61_09455"
     CDS             <1..448
                     /locus_tag="PKJ61_09455"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOC14090.1"
                     /translation="RIDERLRDRELGVLDLLTTLGVANRFPLEAARRQWWGKFYHRAP
                     GGESWADVALRLRSFLRDLADSPAERAVLFVHDAVVMLVRYILENLDEHAVLRIRATD
                     PVRNVSITVLIRDGVDWRLDRYNDVSHLVSAGAPVTEHPGEPDVAR"
     gene            459..1304
                     /locus_tag="PKJ61_09460"
     CDS             459..1304
                     /locus_tag="PKJ61_09460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HOC14091.1"
                     /translation="MTPRLLREWPLPEAGSSKYLRGRVLVIGGAVRTPGAVQLAGIAA
                     LRVGAGHLSLAVAKPAAVALAVSTPESGVTGLACDHAGSVLSDDLPKLAKALEVTDAV
                     VLGPGLDRSPATAQVVATVLAMLGSETRLVVDALALHPLTGIDALTESLAARMVLTPN
                     DDEASELLGRPLSDDLLADIGSLAARFGTVVACHDLVVQPDGKAWQISVGDSGLATSG
                     SGDVLAGALGGLLARGAEPAQAACWAKYLHAAAGERLASRIGRLGFLAGEIRDELPMV
                     LAEIG"
     gene            complement(1319..2590)
                     /locus_tag="PKJ61_09465"
     CDS             complement(1319..2590)
                     /locus_tag="PKJ61_09465"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HOC14092.1"
                     /translation="MAAGGAYGYLPRVSDALLRARLRAMGAVLIEGVKGCGKTETARQ
                     VAASEILLEVDHNAEQKALLDPRLILDGETPRLLDEWQRVPRVWDAVRRAVDERRMPG
                     QFILTGSATPNDNLQRHSGAGRFGIVDMRTMTLAEKQAITPSVSVAGLLEGVAPEPAN
                     CPMPFRDYLHHLVMGGWPYLVGADEQTADAFLDGYLSIIIDHDIDEVSEATRNPRLVR
                     RFLHAYAQMTAHPANLSSILKRAHDDVDADSNGPSRYAATDYLDALRRMRLVDEVTAW
                     EPSVRSSKRLTSTPKRHLGDVSLAVSLLRMTPDRMLNDLNTTGYLFESLVVHDLRVYA
                     AAERASVHHYRELDGRLEVDCILETQDGDWVGVEVKLGDRDVDGAARSLLRLAERVAR
                     PPKSLAVVTATSLAYTRADGVHVIPLGCLGP"
     gene            complement(2669..>2962)
                     /locus_tag="PKJ61_09470"
     CDS             complement(2669..>2962)
                     /locus_tag="PKJ61_09470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14093.1"
                     /translation="CSTAVRVRRAVAYSFGHSWGMLGAALIPGLILGLSVLGVVSHEH
                     AVWGTLWVDVAILAVLGYLGVATWSRRLWPRLVGAGATALLGVVLIVLKALIH"
     assembly_gap    2963..3062
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3063..4217)
                     /locus_tag="PKJ61_09475"
     CDS             complement(<3063..4217)
                     /locus_tag="PKJ61_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015071611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOC14094.1"
                     /translation="MRTTTESLPGLTITNLTLDAPLRHADPSAGTIEVFARVVTGADG
                     ERKPYLAFLQGGPGHEAPRPSPGSPPWLARALQDYQVVLLDQRGTGRSTPISSTGTEP
                     LAGLSAAQQAEYLTHLRADEIVNDAELVREALGAATWTVLGQSFGGFTTLHYLSVHAE
                     SLAGALITGGLSAVGRPIDDVYAATWQIMIAKSEAYYRRFPGDRDRVRQLSELATAGR
                     IALPNGDLVSAARLRTVGSLLGVQGGIERLHYLLELDHRSSAFAHDLAEALPFSGRNP
                     LYAVLHESSYADGGRTGWSAERTMPDRVREDVTLLGGEHLQRSVFTEDRELAGSAEVA
                     DLLAEHEWNQLYFPERLAAAEVPVAAAVYHQDAFVPLEFSLQTAALLPDCR"
     gene            complement(4341..5468)
                     /gene="chvE"
                     /locus_tag="PKJ61_09480"
     CDS             complement(4341..5468)
                     /gene="chvE"
                     /locus_tag="PKJ61_09480"
                     /inference="COORDINATES: protein motif:HMM:NF040907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HOC14095.1"
                     /translation="MRKFVKVATVLAAAGALALTGCGGGRTETSAPPAASPGATESSA
                     AAAGFDKGSVIGVALPQKTSENWVLAENLFNTQLTAAGYKPSVQFANNGVTEQQNQIQ
                     AMIEQGAKVLIIGAIDGSQLGTQLAAAKQAGITVIAYDRLLTNTPDIDFYVAFDNFKV
                     GQLQGQALLDGLAARKGDVKKWNIELIAGSPDDSNAKVFFDGAMDVLQPKIDDGTLTV
                     VSGQTTFDKVVTQGWKAENAQKRMDTILSGSYEKTDLHGILSPNDTLARAAMTAVDAA
                     GKDLPVVTGQDSEVESVKSIMAGKQYSTIFKDTNKLVEHSIVMVNDLQAGKTPEVNDT
                     ESYNNGVKIVPAYLLAPVIVTKENAAEVYANDATLGPLTKA"
     gene            complement(5552..6838)
                     /gene="gguB"
                     /locus_tag="PKJ61_09485"
     CDS             complement(5552..6838)
                     /gene="gguB"
                     /locus_tag="PKJ61_09485"
                     /inference="COORDINATES: protein motif:HMM:NF040906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOC14096.1"
                     /translation="MDALRKLFGGNMRQFGMLFVLVALTLFFHIMTRGLVVTSTNLMN
                     LLNGNSYILVLAIGMVMVIVIGHIDLAVGSVAAFVGIVVAVAMRDFGLPSWAGVLLGL
                     AIGAICGIWQGFWVAYFGIPGFIVTLAGYMFFRGANQWVGQSLTVPVPKDFQVLGAGY
                     LPEWGPNTGMNNSTLLLGIVAIAVLAWMQLAQRRALIKVNAEVPPMWVTITRIVLLAV
                     VIAYLTWMYGTGRPGTSFPIPGLILVGLVILYSWVTQRTITGRHIYAVGGNRLAAELS
                     GVNTKRTYFLVMMNMSILAAFAGMMFVGRSTASGPFDGVGWELDAIAAVFIGGAAVSG
                     GVGTVIGSIIGGLVMAVLNNGLQLMSVGSQATQMIKGLVLLAAVAIDVWQKRQGRPSI
                     SGLIFRGKNAGQVTPAEVALAESEAQDKTAVAKELR"
     gene            complement(6871..8430)
                     /gene="gguA"
                     /locus_tag="PKJ61_09490"
     CDS             complement(6871..8430)
                     /gene="gguA"
                     /locus_tag="PKJ61_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOC14097.1"
                     /translation="MSQSEVMLEMRHIVKEFPGVKALDGVSLTVRRGEIHAICGENGA
                     GKSTLMKVLSGVHPHGTYTGEIIYEGKPATFSSIRDSEHAGIVIIHQELALIPELSIT
                     ENMFLGNEVRGRWGIDWVVAREKARQLMARVGLQDHPDTQIKSLGVGKQQLVEIAKAI
                     SRDVRLLILDEPTAALNETDSAHLLDLIREFKAQGMTCIMISHKLNEIAAIADEITII
                     RDGHSVETIAVDPNARVDEDRIIRGMVGRPLTSRYPERHPHTGEVMFEVKDWTVAHPE
                     VPDRLVCKGSTFDIRAGEIVGFAGLMGAGRTELARSLFGRSYGTWLGGTMKLRGSQIE
                     PKTVEQAIDLGLAYVTEDRKVLGLNLLDDIRTTVVSAELSKIASRSGVVDRNAEFIAA
                     EQYRKELRIKTPSVNEGVIKLSGGNQQKVVVAKWLFTEPDLLILDEPTRGIDVGAKYE
                     MYTIMNTLAAQGKAIIMISSELPELLGMCDRIYTVSAGRITGCLPVAEASQESLMRLM
                     TLIPTNTENAA"
     gene            8766..9926
                     /locus_tag="PKJ61_09495"
     CDS             8766..9926
                     /locus_tag="PKJ61_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017934302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HOC14098.1"
                     /translation="MRTSPERTSARNRTREDIYGHIRAAGKLTRSSLVEMSGLSPSTV
                     GHAVSWLLAEGRIMETEMQGKGPGSGSGRPATMLQVTPSDIPIAAIDFGHSHIRVAVG
                     SGSGEIIDESHIQLDVDLLALESLDLAAKSLDGLRRQHGMTTFGAVVAGTPGPVDPLN
                     GVVRSPTILSSWVGIAPKQELERRLGTVVHIENDATMGALGELRHGIGRSHKDFLYVK
                     ASHGIGAGLVISGQVYRGATGLAGEIGHTLLVGRSELCRCGNRGCLEAVVAVGPLREQ
                     ILHSHPYSPAGETFEFTDFDDQITDRILNEAGRTLGRVLADLCNLLNPTALIIGGQLG
                     TSTPALVEGVQASVMRYAQPATAAAIEVAPAALGARAELVGALELAGQLASH"
     gene            9950..10657
                     /locus_tag="PKJ61_09500"
     CDS             9950..10657
                     /locus_tag="PKJ61_09500"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOC14099.1"
                     /translation="MARNDVRAPRVLIVEDEPALMGAFSRHLEQLGFEVAQATSIQQA
                     RAAAWEQLPDVVLLDVLLPDGSGYDFCAELRRITSAPIIYVTALGQDAAVVRGLAAGG
                     DDYLTKPFSLDVLTARINAQLRRSGAPRALRIELPPLYVDFVTGKVTLEGVAIALSQK
                     ELQLLGYFAANAGRGLTQDELLDAVWADHSGVPTNTVRQHISNLRRKLSLNGTSAFEL
                     ILTADHRYVFQQVRFTG"
     gene            complement(10647..>11355)
                     /locus_tag="PKJ61_09505"
     CDS             complement(10647..>11355)
                     /locus_tag="PKJ61_09505"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOC14100.1"
                     /translation="MDRAKTDFLAIVAHELNTPLAAIRTHAEEAERHLATGEPVTHEF
                     SVIDSEAERLSRLVGQLLDVARIADGRLDLELRPEHLATMVQQTLRAFMPLCTRAGNT
                     LELARGSASPVVLADRERVAQVLVNLLANAVRHTRAGVITVGITEADGFAELSVRDTG
                     EGMPPEVVARLFEHSLYRTGGLHSARDAGLGLGLQISEHIVEAHGGTLSVTSAVSVGT
                     TVTCSFPLAPADQLVSQ"
BASE COUNT         1879 a         3799 c         3675 g         1902 t
ORIGIN      
        1 ccggatcgac gagcgcctgc gcgaccgcga gctgggcgtc ctcgacctgc tgacgacact
       61 gggggtggcc aaccggttcc cgctggaggc cgccaggcgg cagtggtggg gcaagttcta
      121 ccaccgtgcg cccggcgggg agtcgtgggc ggacgtggcc ctgcgactcc ggtcgtttct
      181 gcgcgacctg gccgattcac ccgccgaacg ggcggtgctg ttcgtccatg acgcggtggt
      241 gatgctggtg cgctacatcc tggagaacct ggacgagcac gcggtgctgc ggatccgcgc
      301 caccgacccg gtgcgcaacg tgtcgatcac ggtgctcatc cgcgacggtg tcgactggcg
      361 actcgatcgc tacaacgacg tatcgcacct ggtcagcgcc ggtgcgccgg tgaccgagca
      421 cccgggagag cccgatgtcg cccgctgagc cgacgcaagt gaccccgcgc ctgctgcgcg
      481 agtggccgct gccggaagcg ggcagctcga agtacctgcg cggccgcgtc ctggtgatcg
      541 gtggcgcggt gcgaacgccc ggcgcggtgc aactggccgg gatcgctgcc ttgcgggtgg
      601 gtgcgggcca tctgagcctt gcggtcgcga aaccggctgc ggtggcgttg gccgtatcca
      661 ccccggagtc aggggtgacc ggactggcct gcgaccacgc cggctcggtg ctctccgacg
      721 acctgcccaa gttggccaag gccctagaag tgacggacgc ggtggtgctc ggccccggcc
      781 tggatcgcag tccggctacg gcccaagtgg ttgcgaccgt gcttgccatg ctgggttcgg
      841 agacgcgcct ggtggtggat gcgctcgccc tccacccact gaccggcatc gacgccctca
      901 ccgagtcact ggccgcccgg atggtactca ccccgaacga cgacgaagcg tccgagttgc
      961 tgggcagacc gctgagcgac gacctcctgg ccgacatcgg ctcgctcgct gcccggttcg
     1021 gcacggtggt ggcctgccac gatctggtcg tgcaaccgga cggcaaggcc tggcagatca
     1081 gcgtcggcga cagcggcttg gccacctcgg gcagcggcga cgtgctggcc ggcgcgctcg
     1141 gcggcctgct cgcccgcggc gcggaacccg cccaagccgc ctgttgggcg aagtatctgc
     1201 acgccgccgc cggtgaacgc ctggcctccc ggatcggacg cctcggcttc ctggccggcg
     1261 agatccgcga tgaactgccg atggtcctgg ccgaaatcgg ctgagccggg gcgtcggact
     1321 atggcccgag acaacccagc ggaatgacat gcactccgtc cgcacgggtg taggccaggg
     1381 atgtcgcggt caccacagcc aacgacttcg gcggtcgtgc gactcgctca gccagccgta
     1441 gcagcgaccg cgcagcgcca tcgacgtcgc ggtcgccgag cttgacctca acccccaccc
     1501 agtcaccgtc ctgagtctcg aggatgcagt ccacctcaag ccgaccgtcc aactcgcgat
     1561 agtggtgaac gctggctcgc tcggctgccg catagacgcg aagatcgtgc acgacgaggc
     1621 tctcgaacag atagccggtg gtgttcaagt cgttgagcat acggtcgggg gtcattcgaa
     1681 gtaaggacac ggcaagcgat acgtcaccga ggtggcgctt cggcgtcgaa gtcaggcgct
     1741 tggacgatcg gaccgagggt tcccaggcgg tcacctcgtc cactagccgc attcgccgta
     1801 gggcatccag ataatcggtg gcggcgtatc gactcggtcc attggagtct gcgtccacgt
     1861 cgtcgtgggc acgtttcagg atggaactca gattcgcggg gtgcgcggtc atttgggcgt
     1921 aggcgtgcag gaatcgacgc acgagtcgcg ggtttctggt ggcttcagac acttcgtcga
     1981 tgtcgtgatc gatgatgatg ctgagatacc catccagaaa tgcgtccgct gtctgctcat
     2041 cagcgccgac gaggtacggc caaccgccca tcaccaggtg atgtaggtag tcgcgaaatg
     2101 gcatcggaca gttcgccggc tcgggagcga ctccttcaag cagtcctgcc acggataccg
     2161 agggcgtgat tgcctgcttt tcggcaaggg tcatcgtccg catgtccacg atgccgaagc
     2221 gcccggcgcc agagtggcgc tgcaaattgt cgtttggcgt ggccgagccg gtgaggatga
     2281 actggcctgg catcctccgc tcgtcaacgg cgcgacggac cgcgtcccac acccggggga
     2341 cacgctgcca ttcgtcaagc aatctgggcg tttcgccgtc gaggatgagc cgtggatcga
     2401 gcaaggcctt ctgctcggcg ttgtgatcga cttcgagcag gatctcgctt gccgctacct
     2461 gcctcgccgt ttcggtcttg ccgcagcctt tgacgccttc gatgagcacc gcccccatgg
     2521 cccgcaagcg ggcgcggaga agagcgtcgg atacccgtgg gagatagccg taggcgcccc
     2581 ctgcagccat gccaccccct caaccaactg attacacgct ctcaaaccaa cagcttacac
     2641 attcttcaag ccacaaccca cacgccattc agtggatcag tgccttgagc acgatcagca
     2701 cgactccgag cagcgcggtc gcgccggcgc cgaccaggcg cggccagagc cgtcggctcc
     2761 aggtggcgac gccgaggtag ccgagcaccg cgaggatcgc cacgtccacc cacagcgtgc
     2821 cccagaccgc atgttcgtgg ctcaccacgc cgagcacgct cagcccaagg atcagtccgg
     2881 ggatcagcgc cgcgccgagc atcccccagg agtggccgaa cgagtaggcc accgcccggc
     2941 gcacccgtac cgcggtggag cannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nngcggcaat ccgggagaag cgccgccgtc tgcagcgaga actccagcgg cacgaacgca
     3121 tcctggtgat agaccgccgc agccaccggc acctcagccg cggccaggcg ctccgggaag
     3181 tacagctgat tccactcgtg ctcggccagc aggtcggcaa cctcggcgga cccggcgagc
     3241 tcgcggtcct cggtgaacac gctgcgctgc aggtgctcac cgccgagcag cgtcacatcc
     3301 tcgcggacgc gatcaggcat cgtccgttcc gccgaccagc cggtgcgccc gccgtcggcg
     3361 tagctggatt cgtgcagcac cgcgtacaac gggttgcgtc cgctgaacgg cagggcctcg
     3421 gccaggtcgt gggcgaaagc ggacgagcgg tggtcgagtt cgagcaggta gtgcaggcgc
     3481 tcgatgccgc cctgcacgcc gagcaggctg ccgaccgtcc ggaggcgcgc ggccgaaacc
     3541 aggtcgccgt tcggcagcgc gatccgtccc gcggtggcca gctcgctgag ttggcggacg
     3601 cggtcccggt cgccgggaaa gcgccggtag taggcctcag acttggcgat catgatctgc
     3661 caggtggccg cgtacacgtc gtcgatcgga cggccgaccg cgctcagccc accggtgatc
     3721 agtgctccgg ccagcgactc ggcatggacg ctcaggtagt gcagcgtggt gaagccgccg
     3781 aaggactggc cgagcaccgt ccaggtggcc gccccgagcg cttcgcgcac cagttcggcg
     3841 tcgttcacga tctcgtcggc gcgcaggtgg gtcaggtatt cggcttgctg ggcggcgctc
     3901 agaccggcca gcggttcggt gccggtgctg gagatcggcg tcgagcgtcc ggtgccgcgc
     3961 tggtcgagca gcacgacctg gtagtcctgc agcgcccgcg ccaaccacgg cgggctgccc
     4021 ggcgagggcc gcggcgcctc gtggcccggg ccgccctgca ggaaggccag atacggcttg
     4081 cgctcgccgt ccgcgccggt gaccacgcgg gcgaagacct cgatcgtgcc ggcggacggg
     4141 tcggcgtggc gcagcggagc gtccagggtg aggttggtga tggttagtcc gggcaggctc
     4201 tcggtcgtgg tgcgcatcca gccaagctat cgcgaaggtt gggtggtctc gacaagctcg
     4261 accagccaaa ggcgaaacgc cgtgggaacc agcgaactgc tggctcccac ggcgttctgc
     4321 ctgggtgaag ctagtgccga tcaggccttc gtgagcgggc ccagcgtggc gtcgttggcg
     4381 taaacctcag ccgcgttctc cttggtgacg atgaccggag ccagcaggta agccgggacg
     4441 atcttcacac cgttgttgta ggactcggtg tcgttgacct caggcgtctt gccggcctgc
     4501 aggtcgttca ccatgacgat cgagtgctcg accagcttgt tggtgtcctt gaagatggtc
     4561 gagtactgct tgccggccat gatcgacttc accgactcca cctcggagtc ctgaccggtg
     4621 acgaccggga ggtccttgcc cgcagcgtca acggcggtca tcgccgcacg ggcgagggtg
     4681 tcgttcgggg acaggatgcc gtggaggtcg gtcttctcgt acgagccgga gaggatggtg
     4741 tccatccgct tctgcgcgtt ctcggccttc cagccctggg tgactacctt gtcgaaggtg
     4801 gtctggcccg aaaccacggt cagcgtgccg tcgtcgatct tcggctgcag cacgtccatg
     4861 gcgccgtcga agaacacctt ggcgttggag tcgtcggggc taccggcgat caactcgatg
     4921 ttccacttct tcacgtcgcc cttgcgcgcg gcgaggccgt ccagcagggc ctggccctgc
     4981 agctggccga ccttgaagtt gtcgaacgcg acgtagaagt cgatgtccgg cgtgttagtc
     5041 agcaggcggt cgtaggcgat cacggtgatg ccggcctgct tggccgcggc gagctgcgtg
     5101 cccagctggg aaccgtcgat ggcaccgatg atcagaacct tggcgccctg ctcgatcatg
     5161 gcctggatct ggttctgctg ctcggtcacg ccattgttgg cgaactggac gcttggcttg
     5221 tacccagctg cggtgagctg cgtgttgaac agattctcgg ccagtaccca gttctcagag
     5281 gtcttctgcg gcagggcgac accgatgacc gagcccttgt cgaaaccggc agcagccgcg
     5341 gacgactccg ttgcgcccgg cgacgccgcc ggcggagccg aggtctcggt acggccacca
     5401 ccgcaaccgg tcagggcaag tgccccggcg gccgcgagca ccgtcgcgac cttgacgaac
     5461 ttcctcatcc catacccttt ctcgatccgg ttcccgcggt gggtaccggc cctatcgccg
     5521 gccgccttgg gtgcggccgg ggagtctgta gttacctgag ctccttggct actgccgtct
     5581 tgtcctgggc ttcgctttcg gcgagtgcca cctcagctgg ggtgacctgg ccggcgttct
     5641 tgccgcggaa gatcagtccg ctgatggagg ggcgcccctg ccgcttctgc cacacgtcga
     5701 tggcgacggc ggcgagcagg accaggccct tgatcatctg ggttgcctgg ctgccgacgc
     5761 tcatcagctg caggccgttg ttgagtactg ccatgaccag gccgccgatg atggagccga
     5821 tcacggtgcc gacaccgccg gagaccgccg cgccgccgat gaacactgct gcgatcgcgt
     5881 ccagctccca gcccacgccg tcgaacgggc ccgaggcggt ggagcggccg acgaacatca
     5941 ttccggcgaa cgcggccagg atcgacatgt tcatcatcac caggaagtag gtccgcttcg
     6001 tgttcacacc cgacagctca gccgcgaggc ggttaccacc aacggcatag atgtgccgtc
     6061 cggtgatggt gcgctgggtg acccaggagt acaggatcac taggccgacc aggatcaggc
     6121 cggggatggg gaacgaggtg cccggacggc cggtgccgta catccaggtg aggtaggcga
     6181 tcaccacggc gagcagcacg atccgggtga tggtgaccca catcggcggc acttcggcgt
     6241 tgaccttgat cagggcccga cgctgcgcga gctgcatcca ggccagcacc gcgatcgcca
     6301 cgattcccag cagcagggtg gagttgttca tgccggtgtt cggcccccac tcgggcaggt
     6361 agcccgcgcc gagtacctgg aagtccttcg gcaccgggac ggtcagggac tggcccaccc
     6421 actggttcgc gccacggaag aacatgtagc cggccagggt gacgatgaag cccgggatac
     6481 cgaagtaggc cacccagaag ccctgccaga tgccgcaaat ggctccgatg gccaggccga
     6541 gcaggacgcc ggcccaactc ggaaggccga agtcacgcat cgcgaccgcg accacgatgc
     6601 ctacgaaggc ggccaccgaa cccacggcca ggtcgatgtg gccgatcacg atcaccatca
     6661 ccatgccgat cgccagcacc aggatgtagc tgttgccgtt caacaggttc atcaggttgg
     6721 tcgaggtgac caccaggccc ctggtcataa tgtggaagaa cagcgttagt gcgaccagca
     6781 cgaacaacat gccgaactgg cgcatgttcc cgccgaacaa cttcctcagt gcgtccattg
     6841 cagtccttct cgaatcggtg tgatcactgg tcaggcggcg ttctcggtgt tggtcgggat
     6901 cagcgtcatc agccgcatca gcgactcctg gctggcctcg gccaccggca ggcagccggt
     6961 gatccgaccg gcgctgacgg tgtagatccg gtcgcacatg cccaacagtt cgggcagctc
     7021 ggaggagatc atgatgattg ccttcccctg cgcggcgagg gtgttcatga tcgtgtacat
     7081 ctcgtatttg gcgccgacat cgatgccccg ggtcggctcg tccaggatca gcagatccgg
     7141 ctcggtgaac aaccacttgg ccaccaccac cttctgctgg ttgccgccgg acagcttgat
     7201 caccccctcg ttcacgctcg gggtcttgat ccgcaactcc ttgcggtact gctcggcggc
     7261 gatgaactcc gcgttccggt cgaccacccc gctgcggctg gcgatcttgc tcaactcggc
     7321 cgaaaccacc gtggtgcgga tgtcgtccag caggttcagg ccgagcacct tgcggtcctc
     7381 ggtcacatag gccagaccca ggtcgatcgc ctgctccacg gtcttcggct cgatctggct
     7441 accgcgaagc ttcatcgtgc cgcccagcca ggtgccatac gagcgtccga acagggaacg
     7501 ggccaactcg gtgcggccag cgcccatcag cccggcgaac ccgacgatct caccggcccg
     7561 gatgtcgaag gtcgaaccct tgcagaccag ccgatccggc acctccgggt gggctaccgt
     7621 ccagtccttc acctcgaaca tcacctcacc cgtgtgcggg tgccgttcgg ggtagcggga
     7681 cgtcagcggt cggccgacca tgccccggat gatccgatcc tcgtccaccc gggcgttcgg
     7741 gtcgaccgcg atcgtctcca cactgtgtcc gtcgcggatg atcgtgatct catcagcgat
     7801 cgcggcgatc tcgttcaact tgtgcgagat catgatgcac gtcatgccct gggccttgaa
     7861 ctcgcggatc aggtcgagca gatgcgccga atcggtctca ttcagagcag ccgtgggctc
     7921 gtccagaatc aacaaccgaa catctcggga aatcgccttc gcaatctcga ccaactgctg
     7981 cttgcccaca cccaacgact tgatctgggt gtccggatgg tcctgcaagc ccacccgggc
     8041 catcaactgc cgcgccttct cccgcgccac gacccaatcg atcccccacc gcccgcgcac
     8101 ctcgttgcca aggaacatgt tctcggtgat cgacagctcc gggatcaacg ccagctcctg
     8161 atgaatgatc acgatcccgg catgctcaga atcacgaatg ctggaaaacg tcgccggctt
     8221 gccctcatag atgatctcac cggtgtaggt gccgtgcggg tgcacacccg acaacacctt
     8281 catcaaagtc gacttacccg cgccgttctc gccacagatc gcgtgaatct caccccggcg
     8341 caccgtcaac gacaccccgt ccaaagcctt cacaccaggg aactccttga cgatgtgccg
     8401 catctccagc atcacttcag attggctcat ccacctgcct ccagctccga tgaaggggcg
     8461 gcagcgctca gcaacatgcg ttcccccttc gaccacgtcg tcaaccggac ctgccttcgg
     8521 ccccagcgcc cggcgcgccg cggacggacc cgcggtggtc ggataggccg aacacaagcc
     8581 gaaggtttca tgctgtcaac gcgggataac aagtcgttat caaaccgttt ctcttcatca
     8641 ttgagttaaa gtcacccgcg gtccgccgcg ttcaccccgg caagggcgct tgtccttgaa
     8701 tcgttcaaca cgaattgatc agtggttggc tgaaaactgt gcacttagga gttaagcttg
     8761 cccccatgcg tacgtcgcct gagcgcacgt ccgcccgcaa tcggacgcgg gaggatatct
     8821 acggccacat ccgtgccgcc ggcaagctca cgcgttccag cttggtcgaa atgagcggac
     8881 tatcccccag cactgtcggc catgccgtct cctggctgct cgccgagggc cggatcatgg
     8941 agaccgagat gcagggcaag ggaccggggt cgggcagtgg ccggccggcg acgatgttgc
     9001 aggtgacccc gagcgacatc ccgatagccg ccatcgactt cgggcacagc cacatccggg
     9061 tggcggtcgg cagcggctcc ggcgagatca tcgatgagtc ccacatccag ctggacgtgg
     9121 acctgctcgc gcttgaatcc ctcgatctgg cggcgaagag cctggacggg ttgcgccgcc
     9181 agcacggaat gacgaccttc ggcgcagtgg ttgcgggcac cccagggccg gttgatccac
     9241 tcaatggcgt cgtccgctcc ccgaccatcc tgtccagctg ggtgggtatc gcgccgaagc
     9301 aggagttgga acgccggctg ggcacggtcg tccacatcga gaacgacgcc accatgggcg
     9361 ccctcggcga actccggcac ggcatcggac gtagccacaa ggactttctc tacgtgaagg
     9421 cttcgcacgg catcggcgcg ggactggtga tcagcggtca ggtctaccgg ggcgcgaccg
     9481 gcctggctgg cgaaatcgga cacacccttt tggtcggacg ctccgagctc tgtcgctgcg
     9541 ggaaccgcgg ttgcctggag gccgtggtgg cggtgggtcc gctgcgcgaa cagatcctgc
     9601 attcgcatcc gtacagcccg gcaggcgaga cgttcgagtt caccgacttc gacgaccaga
     9661 tcactgatcg catcctcaac gaagccggcc gcacgctggg ccgggtgctg gccgatctgt
     9721 gcaacctgct gaacccgacc gcgctgatca tcggcggcca gctcggcacc agcacgccgg
     9781 ctttggtcga aggcgtccag gcctcggtga tgcggtacgc ccagcccgcc actgcggcgg
     9841 cgatcgaggt cgcccccgcc gccctgggcg cccgcgcgga gttggtcggc gccctggagc
     9901 ttgccgggca actggcgagc cattgagctc cggggggaag ggtcggccga tggcgcgcaa
     9961 cgacgtgcgg gcaccgcgtg tgctcatcgt cgaggacgag cctgccctga tgggtgcctt
    10021 ttcccggcac ctggagcagc tcggtttcga agtggctcag gcgaccagca tccagcaggc
    10081 ccgcgctgca gcctgggagc agttgccgga cgtggtgctg ctcgatgtgc tgctgccgga
    10141 cggctccggc tacgacttct gcgccgagct gcggagaatc acgtccgccc cgatcatcta
    10201 cgtgaccgcg ctcggccagg acgctgccgt cgtccgcggg ctggccgccg gcggcgacga
    10261 ctatctcacc aaaccgttca gcctcgacgt actcaccgcc cggatcaacg cccaacttcg
    10321 tcgctccggt gccccacggg cgctgcgaat cgaactaccg ccgctctacg tcgatttcgt
    10381 cactggcaaa gtcaccctgg agggggtcgc gatcgcgctc tcccagaagg aactgcagct
    10441 gctcggctac ttcgccgcca acgctggccg aggactcacc caggacgaac tactggacgc
    10501 ggtctgggca gaccattccg gggtgccgac gaacaccgtc cgccagcaca tctcgaacct
    10561 gcggcgaaag ctgagcctta acggcaccag cgcgttcgaa ctgatcctga ccgcagatca
    10621 ccgctacgta ttccagcagg tccgcttcac tggctgacca actgatcggc cggtgcgagc
    10681 gggaagctgc aggtcaccgt cgtcccgacg ctgacggcgg aggtcaccga aagcgtgcca
    10741 ccgtgcgcct cgacgatgtg ctctgagatc tgcagcccca ggccaagccc ggcgtccctc
    10801 gccgagtgca gcccgccggt ccggtagagc gagtgctcga acagccgggc cacgacctct
    10861 ggcggcatgc cttcaccggt gtcacggacg gacagctccg cgaacccgtc cgcctcggtg
    10921 atgccgacgg tgatcacccc cgcacgggtg tgccggacgg cgttcgcgag caggttgacc
    10981 agcacctgcg cgacccgctc gcggtcggcc aggacgaccg ggctcgcgct gccgcgggcc
    11041 agctcgaggg tgttgccggc acgggtacac agcggcatga acgcccggag ggtctgctgg
    11101 accatagtgg ccaggtgctc cgggcggagc tcgaggtcga ggcgtccgtc cgcgatcctg
    11161 gcgacgtcga gcagttggcc caccagccgg ctcagccgct cagcctcgga gtcgatcacc
    11221 gagaactcgt gagtcaccgg ttcgccggtg gccaggtgcc gctcggcctc ctctgcatgc
    11281 gtccggatcg ctgcgagcgg ggtgttgagc tcatgcgcga cgatcgcgag gaagtcggtc
    11341 ttcgcgcgat ccatc
//
LOCUS       DAOPRQ010000072        11332 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_4816_length_11332_cov_10.322338, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000072 DAOPRQ010000000
VERSION     DAOPRQ010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 11332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11332
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4816_length_11332_cov_10.322338"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            107..277
                     /gene="rpmG"
                     /locus_tag="PKJ61_09510"
     CDS             107..277
                     /gene="rpmG"
                     /locus_tag="PKJ61_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HOC14101.1"
                     /translation="MAKKQADVRPKITLACTQCKERNYITKKNRRNDPDRMELKKFCP
                     KCHVHTAHRETR"
     gene            414..842
                     /locus_tag="PKJ61_09515"
     CDS             414..842
                     /locus_tag="PKJ61_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013160465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HOC14102.1"
                     /translation="MPISEEHVGRSYPATAPYQVSRAKIAEFAAALGDAANPAYAGTD
                     PIAPPTFAVVLAAAAWDAMFDDPDLELSLSRTVHADQRFAWQRPLRAGDEVTATLRIE
                     KVRARGPMAIITIAVDLATAAGEELCTATSTLMHTQEAAE"
     gene            839..1246
                     /locus_tag="PKJ61_09520"
     CDS             839..1246
                     /locus_tag="PKJ61_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012923738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HOC14103.1"
                     /translation="MSIEASVGQELPGFTRTFTRADLVRYAGASGDFNPIHWSNPAAA
                     ALGLPDVIAHGMLTMGTALRVVTDWTGDPARVLNYFVRFSKPVVVPETDGAEVRFSGT
                     VTKVEDGVATVRIEAVAGEVAVLGAATAEVSVG"
     gene            1239..2276
                     /locus_tag="PKJ61_09525"
     CDS             1239..2276
                     /locus_tag="PKJ61_09525"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HOC14104.1"
                     /translation="MAELLADHTTFRVGGPARAWAEPETEAELIDLVSSADARGEPVL
                     ILGGGSNVLVADAGFDGTVIQLGRLRGVHTEDVAACSGAFIDVAAGEPWDAFVERSVH
                     AGHVGVEALSGIPGLVGATPIQNVGAYGQEVSQTIARVRLWDRQDRKVVTRSVAECHF
                     GYRRSLFKAQPGRWVVLSVSFQFRLGELSTPVRYAELAKALGIQPGERAPLAKVRRTV
                     LQLRRGKGMVLDQADADTWSAGSFFTNPLVPAEIAAALPDDAPRYPQPDGTVKTSAAW
                     LIEHAGFSRGYGLGLARLSSKHTLAITNRGSAQAADIVALAREVRAGVERAYGVRLVP
                     EPVLIGVSLDA"
     gene            2343..3533
                     /locus_tag="PKJ61_09530"
     CDS             2343..3533
                     /locus_tag="PKJ61_09530"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016455973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid aminotransferase"
                     /protein_id="HOC14105.1"
                     /translation="MSILSSLPLAPRDPILGLTEAFVADTRADKVNLGVGVYLGEDGK
                     IPLLDCVREAEARLAAAPAPRGYLPIDGLPAYTKGVRELVFGAESDAVTSGRVVTVQG
                     LGGTGALKIGAEFLKLTDAGRTLLISDPSWENHQALFSRAGYDVRTYRYYAPALGGID
                     FQGMLADLRAAEPGTVVVLHACCHNPTGYDLDDAQWAQVADVVVERGLVPFLDMAYQG
                     FADGIEPDGAVVARFAGLGVPLLVASSFSKSFSLYGERVGGLSVLAADAEEAARVLSQ
                     LKVTIRTTYSNPPTHGAAVVASVLGDPELRARWEAELESMRLRIKAMRSALVSELAGA
                     PRDFGFIADQVGMFSYCGLSAEQMRELREVHGVYGTDAGRICVAALNPSNVGTVAKAI
                     AAVL"
     gene            3685..3757
                     /locus_tag="PKJ61_09535"
     tRNA            3685..3757
                     /locus_tag="PKJ61_09535"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3718..3720,aa:Trp,seq:cca)
     gene            3822..4232
                     /gene="secE"
                     /locus_tag="PKJ61_09540"
     CDS             3822..4232
                     /gene="secE"
                     /locus_tag="PKJ61_09540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HOC14106.1"
                     /translation="MADEQDQAAADVPADPTDSLDLEAMGLEDVELTEEEQVAQAAAQ
                     SRPVRKAATPDAGKGHATPKRRTPKVNSDEPRRATPAEFVQEAVAELRKVVWPTGSQL
                     QQYFLVVLIFVLIMIGIVTALDLLFGAGILAIFG"
     gene            4250..5065
                     /gene="nusG"
                     /locus_tag="PKJ61_09545"
     CDS             4250..5065
                     /gene="nusG"
                     /locus_tag="PKJ61_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014847293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HOC14107.1"
                     /translation="MTENNTPGDEFGTENEIEIDLGLPDVGTEDDLELNLDFGGTIDA
                     EADDIVLDLGDESEPEPEPETVSDADEEAVLEELRQRLRSQIGDWYVIHTYSGMENRV
                     KQNLDNRVKTLGMEDYIFEAVVPTEEVIETRNGARKSVTRTVLPGYVLVRMDLTDASW
                     AAVRHTPSVTGFVAHANAPVPLSLDEVEKMLAPSALAKAAAASVGTKRSAKKIEIADF
                     KVGDSVMVVQGPFAGVHATITEINANSQRLKALVEILGRETPVDLTLGQIQKV"
     gene            5135..5566
                     /gene="rplK"
                     /locus_tag="PKJ61_09550"
     CDS             5135..5566
                     /gene="rplK"
                     /locus_tag="PKJ61_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016666126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOC14108.1"
                     /translation="MPPKKKVAAIVKVALNAGAATPAPPVGTALGPHGVNIMEFCKRY
                     NAETEPMRGNVIPVEITIYEDRTFTFITKTPPAAELIKKAAGLKSGSARPHKDKVAHL
                     SADQVREIANTKLPDLNANDIDAAMKIVAGTARSMGVTVDL"
     gene            5647..6348
                     /gene="rplA"
                     /locus_tag="PKJ61_09555"
     CDS             5647..6348
                     /gene="rplA"
                     /locus_tag="PKJ61_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HOC14109.1"
                     /translation="MKHSKAYKAQIANIDEAQLYSPEAALGIVKKNASAKFDETVEVA
                     LRLGVDPRKADQMVRGTVNLPNGTGKTARVLVFATGEKAADAIAAGADEVGADELIDK
                     IAGGYLDFDAVVATPDLMGKVGRLGRVLGPRGLMPNPKTGTVTMDVAKAVTDIKGGKI
                     EFRVDRHANLHFIVGKASFTDQQLIENYFAALDEVMRLKPSASKGRYLKKATISSTMS
                     PGVGVDPTKLKPAAE"
     gene            6677..7261
                     /gene="rplJ"
                     /locus_tag="PKJ61_09560"
     CDS             6677..7261
                     /gene="rplJ"
                     /locus_tag="PKJ61_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020388132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOC14110.1"
                     /translation="MARPDKAAAVAELAEQFSNSAAAVLTEYRGLTVKSLKELRRALG
                     EDASYAVTKNTLTMIAAREAGIEGLDDQLAGPTAIAFIRGDVATVAKGLRDFAKANPL
                     LVIKGGVMDGRVLDAESVKKLADLESREVLLAKLAGAMNGNLAKAVGLFAAPLSQAAR
                     ALDALKSQKEAGDAPATDAAPAAEAPDAADAASE"
     gene            7280..7672
                     /gene="rplL"
                     /locus_tag="PKJ61_09565"
     CDS             7280..7672
                     /gene="rplL"
                     /locus_tag="PKJ61_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014167065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HOC14111.1"
                     /translation="MAKLTTDELLEAFKEMTLIELSEFVKLFEDTFGVTAAAPVAVAA
                     AAAPAAGGDAAEEEEVSNEVDVILDSAGDKKIQVIKEVRALTNLGLKEAKDLVEAAPK
                     VVLEKVTRDAAAKAKETLEAAGAAVTIK"
     gene            complement(7965..8126)
                     /locus_tag="PKJ61_09570"
     CDS             complement(7965..8126)
                     /locus_tag="PKJ61_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14112.1"
                     /translation="MSSVRKVLAVAAVTGVFAVLPITTSGIQSAGPVVGGGTSTTTNG
                     GQGSWPLKR"
     gene            8248..9516
                     /locus_tag="PKJ61_09575"
     CDS             8248..9516
                     /locus_tag="PKJ61_09575"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOC14113.1"
                     /translation="MSATDAARFGAMLRESRRSLGLSQAELGGGKYSGSYISHLESGR
                     RVANAEAIEFLSRRLGVSLLEWGVTATGDSPAQAVVADAIEDLLVAERASSQHDWAAA
                     TAHATKAAEIAFAAGDMSRHWESLYVLAQTRHASGEFLEAAHLAEQLSEHETALKFGV
                     ARAQALSLASIGYRASDRLGWAVAFGARAVEAASSAPPVILSEALMALVSAMSEAGHS
                     AAESEPYLRRLHELSPRLNSSHSRGIIAWTVGVAAFVAGDQEAGLRYFEEAKHLLEPQ
                     RDLRMWFRYHSIAARWRLNTGLLDGVESMIQTASTGLAFLGNTYDLVELRQVEAMLAL
                     RKGDAVEAARLMSEVLDDPVMGADGMSRGGSELLLAHAFEALGRNESARRRYQLAAMQ
                     FEVEGRFRAAVDAWRRSAGEETSNSFLTQG"
     gene            9895..>11332
                     /locus_tag="PKJ61_09580"
     CDS             9895..>11332
                     /locus_tag="PKJ61_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOC14114.1"
                     /translation="MAVSRTASKNTNVVSSSGRISFAKIHEPLEVPNLLDLQVESFDW
                     LIGNETWQNRVAAAQAVGRTDVNTKSGLEEIFEEISPIEDLSQTMSLSFRDNRFEEPK
                     HTVEECKDQDFTYAAPLFVTAEFMNNDTGEIKSQTVFMGDFPLMTDKGTFIINGTERV
                     VVSQIVRSPGVYFEQTADKTSDKDIFTCKIIPSRGAWLEFEIDKRDMVGVRVDRKRKQ
                     NVTVLLKALGWSNEQILAEFGEYESMRLTLEKDHTANTDEALLDMYRKLRPGEPPTRE
                     AAQQMLENYYFNPKRYDLAKVGRYKINKKLQTGLPFDTQVLTIDDIVAAIRYIVALHE
                     GRTELGEMIVETDDIDHFGNRRLRTVGELIQNQLRIGLGRLERTVRDRMTTQDIEAIT
                     PQTLINIRPVSAALKEFFGTSQLSQFMDQTNPVAGLTHKRRLSALGPGGLSRDRAGME
                     VRDVHHSHYGRMCPIETPEGPNIGLIGSL"
BASE COUNT         2050 a         3742 c         3709 g         1831 t
ORIGIN      
        1 gcgggatagc tcaggcggta gagcaagcgg ctcataatcg ctgtgtcgcg ggttcaagtc
       61 ccgctcccgc taccaaaagt agaactcgca agggaaagag gaagtcatgg ccaagaagca
      121 agccgacgtt cggcccaaga tcaccttggc atgcacccag tgcaaggaac ggaactacat
      181 caccaagaag aatcggcgca acgaccccga tcgcatggag ttgaagaagt tctgcccgaa
      241 gtgccacgtg catactgcgc accgcgagac ccgctgaccc aagcctcatc gagccgcccg
      301 ccgaccgcgg gcggcttcgt tgcgtttgcc caccaacggt ctgcacgatc ctgaaccgtg
      361 ttcggtgcag gatcatgcac gccgatgaga cttctgccgg ataacctgtg ccaatgccga
      421 tcagcgaaga acacgtcggg cgcagctacc cggcgaccgc gccctatcag gtgagccgag
      481 ccaagatcgc cgagttcgcc gccgccctcg gggatgccgc caacccggcc tacgccggca
      541 ccgacccgat cgccccgccc accttcgcag tggtgttggc cgcggcagcc tgggacgcga
      601 tgttcgacga tcccgacctc gaactcagcc tcagccggac ggtgcacgcc gaccagcgct
      661 tcgcttggca gcggccgctc cgagccggcg acgaggtgac cgccaccttg cgaatcgaga
      721 aggtaagggc tcgcggcccg atggcgatca tcacgatcgc ggtcgacctg gccaccgcag
      781 ccggcgagga actctgcacg gccacgtcca ccctgatgca cacccaggag gcagcggaat
      841 gagcatcgaa gcgtccgtcg gccaggaact gcccggcttc acccgtacct tcaccagagc
      901 agatctggtc cgctacgccg gtgcgtccgg cgatttcaac ccgatccact ggtcgaaccc
      961 tgcggccgcg gcgctcggcc tgccggatgt gatcgcccac ggcatgctca ccatgggcac
     1021 cgcgctgcgc gtggtcactg actggacggg cgatccggcc cgggtgctca actacttcgt
     1081 ccgtttcagc aagccggttg tcgtccccga gaccgacggg gccgaggtcc gcttcagcgg
     1141 cacggtgacc aaggtcgagg acggggttgc gacagtgcgc atcgaggccg tcgccggtga
     1201 agtggccgtg ctcggcgcgg cgaccgcaga ggtgtctgtt ggctgaactg ctcgccgacc
     1261 acaccacctt ccgggtgggc ggtccggccc gagcctgggc agagcccgag accgaggctg
     1321 agctgatcga cctggtcagc agcgcggacg ccaggggcga gccggtactg atcctcggcg
     1381 gcggctccaa cgtgctggtc gcggacgcgg gcttcgacgg cacggtgatc cagctcggac
     1441 gcctgcgcgg cgtgcacacc gaggacgtgg ccgcctgttc gggcgcgttc atcgacgtgg
     1501 ccgccggtga accctgggac gcgttcgtcg agcgctcggt gcacgccggc cacgtcggcg
     1561 tggaggcgct ctcgggcatc cccggcctgg tcggggccac cccgatccag aacgtcggcg
     1621 cctacggcca ggaggtcagc cagaccatcg cccgcgtccg gctctgggac cgccaggatc
     1681 ggaaagtggt cacccgcagc gtcgccgaat gccacttcgg ctaccggcgt tcgctgttca
     1741 aggcccagcc cggacgctgg gtggtgctca gcgtgagctt ccagttccgc ctaggcgaac
     1801 tgtcgacgcc ggttcgctac gccgagcttg cgaaagcgct gggcattcaa ccgggggagc
     1861 gcgcgccact ggccaaggtg cggcggacgg ttctccaact gcgccgcggc aagggcatgg
     1921 tgctcgatca ggctgatgcc gacacctgga gtgccggcag tttcttcacc aacccgctgg
     1981 tgccggccga gatcgcggcc gcgttgccgg acgacgcgcc gcgctacccg cagcccgacg
     2041 gcacggtcaa gaccagcgcg gcctggctga tcgaacacgc cggtttcagc cgcggctacg
     2101 gcttgggcct ggccaggttg tccagcaagc acacgctggc catcacgaac cgtggctcgg
     2161 cccaggctgc cgacatcgtc gcgctggcgc gcgaagtgcg tgccggtgtg gagcgcgcgt
     2221 acggcgtccg tttggtacct gaaccggtac tgatcggtgt cagcctggat gcgtagactg
     2281 tcgcccgacg actgctgggt cggctcagcc gcgccggcgc ccggtctttg aaaggaaccc
     2341 agatgagcat tctgtcgagc cttccgctgg caccgcgtga cccgatcctc ggcctgaccg
     2401 aggctttcgt cgccgacacc cgggccgaca aggtgaatct gggcgtcggg gtgtacctgg
     2461 gcgaagacgg caaaatcccg ctgctggact gcgtccgcga ggccgaggct cggctggccg
     2521 cagcgccggc cccacgcggc tatctgccaa ttgacgggct gccggcctac accaaaggtg
     2581 tccgcgagtt ggtcttcgga gccgaatcgg acgcggtcac gtccggccgg gtggtgaccg
     2641 tgcagggcct gggcggcacc ggtgcgctca agatcggcgc cgagttcctc aagctcaccg
     2701 atgccggacg cacactgctg atctccgacc cgtcctggga aaaccaccag gcgctgttca
     2761 gccgcgccgg gtacgacgtc cgcacctacc ggtactacgc gccggcgctg ggcggcatcg
     2821 acttccaggg catgctggcc gacctacgtg cggctgagcc aggcaccgtg gtcgtcctgc
     2881 acgcctgctg ccacaacccg accggctacg acttggacga tgcccagtgg gcccaggttg
     2941 ccgacgtcgt ggtcgagcgc gggctggtgc ccttccttga catggcctac cagggcttcg
     3001 cggacggaat cgagccggac ggcgcggtcg tcgcccggtt cgcagggctg ggcgtcccgc
     3061 tgctcgtcgc gtcctcgttc agcaagagct tcagcctcta cggcgagcgc gtcggcgggc
     3121 tgagcgtcct ggccgcggac gccgaggaag ccgcgcgggt gctctcccag ctgaaggtga
     3181 ccatccggac gacgtactcc aacccgccca cccacggcgc ggcggtggtc gcgtccgtcc
     3241 tcggtgaccc ggaactgcgg gcacgctggg aggccgaact ggagtcgatg cggctgcgga
     3301 tcaaggcgat gcgttccgcg ctggtgagtg aattggccgg cgccccgcgt gatttcggct
     3361 tcatcgccga ccaggtgggc atgttcagct actgcgggct gagcgcggag cagatgcgcg
     3421 aacttcgtga ggtgcacggg gtgtacggca ccgacgccgg ccgcatctgc gtggctgcgc
     3481 tgaacccgag caacgtcggg accgtggcca aggcgatagc tgcggtgctc tgagccggcg
     3541 atcggtccat acaccggctg ggcgcgccaa agcccgttcg gttcgactcg cctgatcaaa
     3601 acccgtacaa tctggttctt ggtggccgac tccggttcgc ccatgcccac aaggcagcgg
     3661 caacctcgag caggtcacca ttgaagggca atagctcaat tggcagagca gcggtctcca
     3721 aaaccgcagg ttgggggttc aagtccctct tgccctgctg gacggccgtt ttgcatgaac
     3781 ggttggcaac acccacctag tgaagatgta aggacggccc cgtggcggac gaacaggatc
     3841 aggctgctgc cgacgtacct gctgacccca ccgactccct tgatctggag gcgatgggcc
     3901 ttgaggacgt cgagctgacc gaggaggagc aggtcgcgca ggcggccgcg cagtcccgtc
     3961 cggtccgcaa ggcagccacc ccggacgccg gcaagggcca cgccacgccc aagcggcgca
     4021 ccccgaaggt gaactcggat gagccccggc gggccactcc ggcggaattc gtgcaggagg
     4081 cggtcgcgga actgaggaag gtggtctggc cgaccgggag ccagctgcag cagtacttcc
     4141 tggtggtcct gatcttcgtc ctgatcatga tcgggatcgt gaccgcgctc gacctgctct
     4201 tcggcgccgg gatcctggcg atcttcggct gacggacgga aagcgagaga tgaccgagaa
     4261 caacaccccc ggtgacgagt tcggcaccga gaacgagatc gagatcgatc tcggcctgcc
     4321 cgatgtcggc accgaggacg acctggagct gaacctcgat ttcggcggca cgatcgatgc
     4381 cgaagccgat gacatcgtgc tcgacctggg cgacgagtcc gagcctgagc ccgaaccaga
     4441 gaccgtcagc gacgcggacg aggaggcggt gctcgaagag cttcgccagc ggctgcgtag
     4501 ccagatcggc gactggtacg tcattcacac gtactccggc atggagaacc gggtcaagca
     4561 gaacctggac aaccgggtga agaccctggg catggaggac tacatcttcg aggccgtcgt
     4621 gcccactgaa gaggtcatcg agacccgcaa cggtgcccgc aagtcggtca cccgcacggt
     4681 gctccccggc tacgtcctgg tgcgcatgga cctgaccgac gcttcctggg ccgcggtgcg
     4741 gcacaccccg tccgtcaccg gtttcgtggc ccacgccaac gcgccggtgc cgctcagcct
     4801 ggacgaggtg gagaagatgc tcgcgccgtc cgccctggcg aaggctgcgg ccgcgtccgt
     4861 cggcaccaag cgcagcgcga agaagatcga gatcgccgac ttcaaggtcg gggattcggt
     4921 catggtggtg cagggcccgt tcgccggcgt ccatgcgacc atcaccgaga tcaatgcgaa
     4981 cagccagcgg ctcaaggcgc tggtcgagat cctcggccgg gagaccccgg tcgatctgac
     5041 cctcggccag atccagaagg tctaggcatc gcatcgccgt cggtcacccg gccgcacggc
     5101 agaacagcac caaacgagaa ggacccaatc agaaatgcct cctaagaaga aggttgccgc
     5161 gatcgtcaag gtggccttga acgcgggcgc ggccaccccg gctccgccgg tcggcaccgc
     5221 gctcggcccg cacggcgtga acatcatgga gttctgcaag cgatacaacg ccgagaccga
     5281 acccatgcgc ggcaacgtca tcccggtcga gatcaccatc tacgaagacc gcaccttcac
     5341 cttcatcacg aagaccccgc cggccgcgga actgatcaag aaggccgccg gactgaagag
     5401 cggctccgcc cgtccgcaca aggacaaggt ggcccacctc agcgccgacc aggtgcgcga
     5461 gatcgccaac accaagttgc ccgacctcaa cgccaacgac atcgacgcgg ccatgaagat
     5521 cgtggccggc accgcccgct ccatgggcgt caccgtcgac ctctgaccaa cccccagtgg
     5581 cagggcagtg cgcccacacc acgactggca cgcggcccac aagagggtcg aactaaggag
     5641 accagcatga agcacagcaa ggcctacaag gcccagatcg cgaacatcga cgaagcccag
     5701 ctgtacagcc ccgaggccgc cctcggaatc gtcaagaaga acgcctcggc gaagttcgac
     5761 gagacggtgg aggtcgccct ccgcctcggc gtcgaccccc gcaaggccga ccagatggtg
     5821 cgtggcaccg tcaaccttcc caacggcact ggcaagacgg cacgggtcct cgtcttcgcc
     5881 accggtgaga aggctgcgga cgccatcgcg gccggcgcgg acgaggtcgg cgccgacgaa
     5941 ctgatcgaca agatcgccgg cggctacctc gatttcgatg cggtcgttgc cacccccgac
     6001 ctaatgggta aggtcggccg cctcggccga gtgctcggcc cgcgcggcct gatgccgaac
     6061 ccgaagaccg gcaccgtcac catggacgtg gccaaggctg tcaccgacat caagggcggc
     6121 aagatcgagt tccgcgtcga tcggcacgcc aaccttcact tcatcgtggg caaggcttcg
     6181 ttcaccgatc agcagctgat cgagaactac ttcgccgcgc tggacgaggt catgcgactc
     6241 aagccgagcg cgtccaaggg ccggtacctg aagaaggcca ccatctcctc gacgatgagc
     6301 cctggcgtcg gtgtcgatcc gaccaagctc aagcccgccg ctgagtgatc tgctccgttc
     6361 ggccccgttc cagctctgga gcggggccga actgctttct gtccgcgcgg gcgcaggctg
     6421 tggctcagcg caccgtccac ggtgcacgca gccacagtca gggcgtccaa ctggggtcgc
     6481 gatttggtga cccgggtcgc gtcccgataa ggtgggcaca ccgaagaccg ctggttctcg
     6541 tcctggcttg ccaggaaggt aaatcccgcg caggtgaaac ctgaagcttg agttgttcgc
     6601 cctggttgcc ctgcgcacca gggttttttc gttcctgtgg acttcggtga tccaaacaga
     6661 tgtgggaagg agacccatgg cgaggccgga caaggcagcc gccgttgcgg agctggctga
     6721 gcagttcagc aactctgcgg ccgcagtgct gaccgagtac cgcggactca ccgtcaagag
     6781 cctgaaggag ttgcgcaggg ctcttggtga ggacgccagc tacgccgtga cgaagaacac
     6841 actgaccatg atcgcggccc gcgaagcggg catcgagggc ctcgacgatc agctcgccgg
     6901 gcccaccgcg atcgccttca tccgcggtga cgtagccacc gtggcgaagg gtctgcgtga
     6961 cttcgccaag gcgaacccgc ttctggtgat caagggtggc gtgatggacg gtcgtgtcct
     7021 cgacgccgaa tcggtgaaga agctggccga cctcgaatct cgcgaggtgc tgctggccaa
     7081 gcttgccggt gcgatgaacg gaaacctggc caaggctgtt ggtctgttcg ccgctccgct
     7141 gtcccaggcc gctcgcgccc tggacgccct gaagtcccag aaggaggccg gggacgcccc
     7201 ggcaaccgat gcagcacccg ctgctgaggc ccccgacgcg gctgacgctg catccgaata
     7261 agaagaaagg acgcccatta tggcgaagct cactactgac gagctccttg aggctttcaa
     7321 ggagatgacc ctgatcgagc tctccgagtt cgtgaagctg ttcgaggaca ccttcggtgt
     7381 caccgctgcc gctccggttg ccgtggctgc tgctgccgct ccggctgccg gtggcgacgc
     7441 tgccgaggag gaagaggtca gcaacgaggt cgacgtgatc ctcgactccg ctggcgacaa
     7501 gaagatccag gtcatcaagg aggttcgcgc gctcaccaac ctcggtctga aggaggccaa
     7561 ggatctggtc gaggccgctc cgaaggtagt gctggagaag gtcacccgcg acgcggccgc
     7621 caaggccaag gagaccctcg aggccgccgg cgctgccgtc accatcaagt gacctgagcg
     7681 tttcgcgcca agcggcggtt gcccttcggg gcagccgccg ttttgctttt ccggacgcgc
     7741 aactcggtgc gcggggcccc gtgtttgccc gcaagctgga ccgatcagtg ccgtcttggg
     7801 ccacaactgt ccagcttgtt gggtgggggc ctgaggggcc ggcgtgtcgg gaagatcact
     7861 gacggaaaca acaaggcggc gatccgtttg gatcgccgcc ttgctgactc tactgggggt
     7921 ggagggtaag tctttggggg tgagtcgggc tacgtgatgt gcgctcagcg cttgagcggc
     7981 cagctgccct ggccaccgtt ggtggtggtg ctggtgccgc cgccgacaac tgggccggcg
     8041 ctctggatgc cgctcgtggt gatcggcagc acggcgaaga caccggtaac agccgctacg
     8101 gcgaggacct tgcgaactga actcatggag ggggaccttt ctagagagcg catgtttgaa
     8161 acgactcgat gcgcatgaaa ctaacgatac ttgactgtct tgtcaacttt tgacaaggtg
     8221 tttttgggca agttttggag gtgacccgtg agcgcgaccg acgcggcgcg atttggcgca
     8281 atgcttcgcg agtcccgccg aagcctcggg ctttcccagg ccgagctcgg cggaggcaag
     8341 tattccggca gttacatctc ccatctggag agcggtcggc gggtagccaa cgccgaagcc
     8401 atcgagttcc tttcgcgcag gttgggcgtt tccctcctcg aatggggagt cactgctacc
     8461 ggcgattcgc cagcccaagc ggtcgtggcc gacgctatcg aggatctcct ggtcgcagag
     8521 cgtgcttctt cgcagcacga ctgggcggcg gcgacggcgc acgccacgaa ggcagcagag
     8581 atcgcatttg ccgccggaga catgtcgcga cactgggagt ccctgtatgt cctggcccag
     8641 acaaggcacg cgtcggggga gttccttgag gccgcgcacc tggccgagca gctctccgag
     8701 cacgagactg cgctcaagtt cggagtcgcg cgcgctcagg cgctgagcct cgcgtccatc
     8761 gggtatcgcg cgtctgaccg gctcggttgg gcggtagcgt tcggcgcgag agcggtggag
     8821 gcggcatcgt cggcgccacc cgtgatcctt tcggaagctc tgatggccct ggtcagtgcc
     8881 atgtcggaag cgggccattc ggccgctgaa agcgagccat acctgcgccg gctgcatgaa
     8941 ctctcaccga gactcaactc aagccattcc cgcggcatca tcgcctggac ggtcggcgtg
     9001 gccgcattcg tggccgggga ccaggaagct ggccttcgct actttgaaga ggcgaagcac
     9061 cttctcgaac cccaacgcga tctgcgaatg tggttccgct atcacagcat cgcagcgcgg
     9121 tggcggttga acacgggtct tctggacggg gttgagtcga tgatccagac cgcatcgacg
     9181 ggtctggcgt tcctcggcaa cacctacgat ctcgtggagt tgcgtcaggt tgaggccatg
     9241 ttggcgctac gcaagggaga cgcggtcgag gccgcgcggc tcatgtccga ggtgctggac
     9301 gacccagtga tgggtgccga cggaatgtcg cggggcggct cggaattgct gctcgcacac
     9361 gccttcgaag cgctgggtcg aaatgagtcc gcgcgccggc gctaccagct tgcggctatg
     9421 caattcgagg tggaagggcg cttccgcgcg gccgtggatg catggcgtcg gagcgccgga
     9481 gaagaaacct cgaattcttt cttgactcag ggttgacatg gttggtcaag tctgtttgac
     9541 ttaactcatc gacgtccccc caatgcgtcg agtgagtaca ccacttaggc cggtgtgctg
     9601 cagcgtttgg ctcccctccc cttgccttgc tgcagacacc ggccttgtgg cgtccccggc
     9661 cagaaactcc caaggggggc cgaaaagtta cgaatctgcg atcatgttcg cgtccggtcc
     9721 gccgacgcgg cactgacgac gggggtaggc ttaccgaccc gcagttgcag gtgagcgtgc
     9781 ggataggtaa gctactgctt tgcccaagct catcgacgac ccgtgtgcat ttgacgcggg
     9841 ccgttctgcg tgcaacgaac ccggccctgc cgggaattgc ggaaggactc caccttggcc
     9901 gtctcgcgca ctgcgtcgaa gaacaccaat gttgtctcat ccagcggtcg gatctccttc
     9961 gcgaagatcc atgaaccgct cgaggtcccc aatcttctgg atctccaggt cgagtccttc
    10021 gactggctga tcggcaacga gacctggcag aaccgggtcg ctgccgccca ggccgtggga
    10081 cgcaccgacg tgaacaccaa gtcgggtctg gaggagatct tcgaggagat ctccccgatc
    10141 gaggatctgt cgcagacgat gtcgctgtcg ttccgcgaca accgcttcga ggagcccaag
    10201 cacaccgtcg aggagtgcaa ggatcaggac ttcacctacg ccgcgccgct ctttgtcacc
    10261 gccgagttca tgaacaacga caccggcgag atcaagagcc agacggtttt catgggcgac
    10321 ttcccgctga tgacggacaa gggcacgttc atcatcaacg gcaccgagcg cgtggtcgtc
    10381 tcccagatcg tccgctcgcc gggcgtctac ttcgagcaga ccgcagacaa gacgtccgac
    10441 aaggacatct tcacctgcaa gatcatcccg tcccgtggcg cctggcttga gtttgagatc
    10501 gataagcgtg acatggtcgg cgtccgcgtc gaccgcaagc gcaagcagaa cgtgaccgtg
    10561 ctgctcaagg ccctcggctg gagcaacgag cagatcctgg ccgagttcgg cgagtacgag
    10621 tcgatgcgcc tcaccctcga gaaggaccac accgccaaca cggacgaggc cctgctcgac
    10681 atgtaccgga agctgcgtcc gggcgagccg cccacgcgcg aagccgccca gcagatgctg
    10741 gagaactact acttcaaccc gaagcgctac gacctggcca aggtcggccg ctacaagatc
    10801 aacaagaagc tgcagaccgg tctgcccttc gacacccagg tgctcacgat cgacgacatc
    10861 gtggcggcca tccgctacat cgtcgccctg cacgagggcc gcaccgagct gggcgagatg
    10921 atcgtcgaga ccgacgacat cgaccacttc ggcaatcgtc gtctgcgcac cgtgggcgaa
    10981 ctgatccaga accagctgcg aatcggcctc ggccgcctcg agcgcaccgt ccgcgaccgg
    11041 atgaccaccc aggacatcga ggcgatcacg ccgcagaccc tgatcaacat ccggccggtc
    11101 tccgctgcgc tgaaggagtt cttcggcacc tcgcagctgt cgcagttcat ggaccagacc
    11161 aacccggtcg ccggcctgac ccacaagcgc cgcctctccg cgctcggccc gggcggtctg
    11221 tcccgcgacc gcgccggcat ggaggtgcgc gacgtccacc acagccacta cggccggatg
    11281 tgcccgatcg agaccccgga aggcccgaac atcggcctga tcggctcgct gg
//
LOCUS       DAOPRQ010000073        11080 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_4953_length_11080_cov_12.045079, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000073 DAOPRQ010000000
VERSION     DAOPRQ010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 11080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11080
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4953_length_11080_cov_12.045079"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            591..1751
                     /gene="dnaN"
                     /locus_tag="PKJ61_09585"
     CDS             591..1751
                     /gene="dnaN"
                     /locus_tag="PKJ61_09585"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002512706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOC14115.1"
                     /translation="MKIRLDRDVLAEAVQWAARSLPTRPSVPILAGLLVKASGNQVVM
                     SSFDYETSAKITVNAEVTDDGVALVSGRLLADIARSLPNKPVDITADETKMELVCGTA
                     RFTLQTLPVADYPALPEMPEATGAILSDEFAKAVGQVVVAAGRDELLPVFTGVRMEIE
                     GETLSLLATDRYRMALKELDWNPRSSGGEGAALVPARVLAETAKSMTAGEQVTLSLSS
                     AATGDGLIGFEGTGTAGVRQITTRLLDGEFPKVRHLMNVQAAVSVRANTAEVIAAVKR
                     VGLVAERNTSLRMLIAEGSITLEAATGDQAQAVEALEAVVEDVSGTGLAMTAAGFNPH
                     YLQDALSALDSPYVQFSFTQPGKPCLLTGLNELDGEPNADYKHVIMLMRLPA"
     gene            1880..3064
                     /gene="recF"
                     /locus_tag="PKJ61_09590"
     CDS             1880..3064
                     /gene="recF"
                     /locus_tag="PKJ61_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HOC14116.1"
                     /translation="MFVTHLSIADFRSYQLADVPLEPGVTVLTGANGQGKTNLIEAVE
                     FLATLGSHRVASEAPLVRAGAERSIVRARVQAGLDDARSLLLEIEIIPGRANKASLNR
                     SPLRRPRDLLGALRVVLFSPEDLAIVKGDPSERRRFIDELLVARWPRLAGVRADYDRV
                     LKQRSTLLKSLSARGPRALTEEVEFTLEVWDGQLAALGAEIVAARLTTLAELGPLLAS
                     AYADIAPTNNLAAVAYRSSALDAAEPRAEATASDTIPTTAELTELLTAKMKARRNEEI
                     ARGLCLVGPHRDDLTLTLGDLPTKGYASHGESWSTALALRLASFGLLQADGVVPVLIL
                     DDVFAELDETRRARLAELIAGAEQVLITAAVAADVPSALQGRRFIVAGGTVVAEAPAA
                     DE"
     gene            3061..3534
                     /locus_tag="PKJ61_09595"
     CDS             3061..3534
                     /locus_tag="PKJ61_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002550519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HOC14117.1"
                     /translation="MTETPEHDPQGTDLAKQIARAARGTTPKPPQRKRRPVSPTDEPT
                     PLGDILKEVIDAQGWTREVNVHQLLARWPELVGAVNAEHSTPTGYADTVLTVRADSST
                     WASSLRLIAPQLVARLNEQLGQGTVTRVNVVGPEAPSWKKGRRSVPGRGPRDTYG"
     gene            complement(3618..4349)
                     /locus_tag="PKJ61_09600"
     CDS             complement(3618..4349)
                     /locus_tag="PKJ61_09600"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HOC14118.1"
                     /translation="MSEDSAESTSPMHHAIARELGLEIIDGAWPLEAARTLEDLQSRF
                     GVSRTVAREVSRQLEAMGLVRTRRRFGLVARPMSEWSVLNPLLIDWRLHSSRREEQIY
                     SLTQLRLAVEPAAAESAARFASIHTRARLLPLAAEMRRTGEAGQLHEFMKFDIEFHSR
                     LLKSCGNELFAALSDLVAVVLQGRTELGLMPAQPKPEALAGHEAVAEAVFRGDPEAAR
                     IAMQGILDEVREAFSEAEAARAAAS"
     gene            4508..5035
                     /locus_tag="PKJ61_09605"
     CDS             4508..5035
                     /locus_tag="PKJ61_09605"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HOC14119.1"
                     /translation="MTNTHLVIMGVAGCGKSSVAEALHKQLGWIVAEGDDFHPQANIA
                     KMSSGTPLNDDDRWPWLDLIAEWTATQDASGRSTIVTCSALRRAYRDRLRTAPGRTVF
                     LHLVGTPELLAERLAGRTDHFMPASLLPSQLATLEALEADEAGFAADIDRPVEAIANA
                     ALAELHLNPSDKSHH"
     gene            5051..6385
                     /locus_tag="PKJ61_09610"
     CDS             5051..6385
                     /locus_tag="PKJ61_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013160387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015295 - solute:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015718 - monocarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate:H+ symporter"
                     /protein_id="HOC14120.1"
                     /translation="MTTPLILAALGGIATIVVLIVWLKVHPFLSLMAGSAVMAIFAGV
                     PYPDLFKSFTAGLGSTLSGVGLLIILGAIIGTLLVSSGGADVIVDTILAKTPLQRLPW
                     AMALIAFVVGIPLFFEVGVVILIPVVMFAARRANLPVVLLGIPALAGLSALHGLVPPH
                     PGPLIAIDALGANLGLTLGLGLLVAIPCVIIAGPLLGKVMAKWVPIEARSDLLGSGGP
                     AEGAKRPSFGAALTVVLLPVILMLARTVVEMTGAEENPFGAALVFVGTPLIALTITTI
                     AAMFMLGFLLSKTRDEVSKLVGSAFGPIAGILLIVGAGGGFKQTLVDSGIADVIAKGL
                     ADAALPPLLAGWIVAVLIRLATGSATVATITASGIMAPLAAGLPPTHAALMVLAIGAG
                     SVFLSHVNDAGFWMVKEYFGMSVGQTFKTWSLMETVLSVTGLAGVMLLSLVV"
     gene            6385..8268
                     /gene="edd"
                     /locus_tag="PKJ61_09615"
     CDS             6385..8268
                     /gene="edd"
                     /locus_tag="PKJ61_09615"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008355987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HOC14121.1"
                     /translation="MSPSPAPAVVAEVTERIRRRSRAGRRAYLDRIHADAERADSRTR
                     LSCANLAHAFAASPESERLAVLKAARPNLGIVTAYNDVLSAHRPYEDYPAVIRDEARR
                     GGASAQVAGGTPAMCDGVTQGTVGMELSLFSRDVIAMSTAIALSHDVYDAALLMGICD
                     KIVPGMLIGALSAGHVPCVFVPAGPMGSGIANAAKAKVREAHVRGDASRDELLAVEQQ
                     AYHSAGTCTFYGTANSNQVLLEVMGLHVPGAAFVPPYSPLRDALTRQAVRTALQVRAG
                     GDRFTPIGELVDERSIVNALVTLLATGGSTNHLIHWVAVARAAGIVFDWSDVDQLSGV
                     VPLLARIYPNGQADINDFERAGGTGFVLRELLDLGLLHADVPTVNAGGLAAQALRPEL
                     VEGGLRFAAPPAETPDAAVLRGGADPFDTTGGLALIAGNLGRAVIKVAAVAPEHRVIE
                     APAFVADSQDEVAAAFQRGDLDRDVLVVVRFQGPRANGMPELHRLTAILGVLQSAGHR
                     VALVTDGRMSGASGRIPAAIHLTPEAAVGGPCAKLRTGDLIRLDANAGTIDVLAVDLE
                     QRTRARWDGPHPLGLGRDLFAGMRASVTGAEQGACSWLPFTDDLDPAAADNTTYRRDL
                     VTQ"
     gene            8265..8906
                     /gene="eda"
                     /locus_tag="PKJ61_09620"
     CDS             8265..8906
                     /gene="eda"
                     /locus_tag="PKJ61_09620"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017933006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - 4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0009084 - glutamine family amino acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HOC14122.1"
                     /translation="MTHEPWSGHGPIIPVLVIEDAGIAVDLARALVAGGLSALEVTLR
                     TPAAPHAIAAIAAEVPEATVGAGTLRLPADAALAKDAGATFAVSPGYTQDLGAACREA
                     GLPLLPGVATASELLLAYADGHRFCKFFPAVAAGGVPVLKALAGPFPDVSFCPTGGIT
                     EATAPDFLALATVPVCGGTWLTPAELVTTRDWTGITELARRAAALGREGTSCS"
     gene            8897..9790
                     /gene="gnd"
                     /locus_tag="PKJ61_09625"
     CDS             8897..9790
                     /gene="gnd"
                     /locus_tag="PKJ61_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008355537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HOC14123.1"
                     /translation="MQLGMIGLGKMGHNMAERLRRAGHEVIGYDRNPAVSETTDLAEL
                     VDRLAGPRVLWLMVPAGAPTQATVAELAELLGAGDVVIDGGNSYYKDDAINSALLAEH
                     GVGYLDCGVSGGVWGLENGYGLMVGGEAGEVARVMPIFDALRPDGPRDEGFVHAGAVG
                     AGHYAKMVHNGIEYGLMHAYAEGWELLETNGTVTDVAGCFKAWTRGTVVRSWLLDLLV
                     KALEENPGLADVDEYTTDSGEGRWTLQEAIDNAVPMPVLAASLFARFSSRQPNSPSMQ
                     MVSALRGQFGGHAVVHTPPPA"
     gene            10093..>11080
                     /locus_tag="PKJ61_09630"
     CDS             10093..>11080
                     /locus_tag="PKJ61_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013159943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOC14124.1"
                     /translation="MSEGKACVTDEPIVAAITAAEAAHVAEGSYDASAITVLEGLDAV
                     RKRPGMYIGSTGERGLHHLVYEVVDNAVDEALAGYCDNIIVRLLDGGGIQVVDNGRGI
                     PVKEHPKEKISALTVALTILHAGGKFGDGGYKVSGGLHGVGVSVVNALSSKLTVDVNR
                     DGYHWRQSFSLGDPDAPLEQLEPTEETGTTVTFYASPEIFETTNYSYETLATRFREMA
                     FLNKGLKINIVDERTQESDTDDDDNDDVEQRSQTFQYTDGLIDYVKYLTGSKDTIHPS
                     VIAIEQAAEEQRMSLEVAMQWNTSFNESVHTFANTINTHEGGTHEEGFRAALT"
BASE COUNT         1769 a         3810 c         3697 g         1804 t
ORIGIN      
        1 ccgctgcgct gaccaccctg gccacgccca ggatctaggg acccgccccg ggctagggga
       61 cagaccgaag tccacacctc acagtcgttc accgcagcgt ccgcacgcct cttcctgcgc
      121 ccattcccgg caaccaagtc cgctatgagc tccagatttc gggttgagcg ctcttcgacc
      181 cgctcaaggg tctccatagg ctcaattttc ccggttcaaa acctgaagcg ctctgcccac
      241 aacccgctcc cgcgggcgaa gtccgctgtg caccgctgtg gatatgtctg gggacgaagc
      301 cccctacttg tgaattccca aaacgcaggc tgagagtatc cgcaaatcct ccccgtgtag
      361 ttctctgtta tccacagggt catgcagacc gcgactcgcg atgtgggtgg ggctgacgcg
      421 agttgtccac cgaatccaca gccgctacta cgaggacgaa ttgatagatc tccaatgaac
      481 ttcaaagtcg gcggatgcac agctggggaa acacgtcacc aaaccccgaa gccagagcct
      541 gcgtcgatat gatctactga cccacgcgtg tgagcacaga gggagcaaca gtgaagatcc
      601 gtctcgaccg cgacgtcctg gctgaggccg tgcagtgggc tgcgcgcagc ctgcccaccc
      661 gtcctagcgt cccaatcctg gccggattgc tggtcaaggc cagtggtaac caggtcgtga
      721 tgagcagctt cgactacgag acgtcggcga agatcacggt caacgcggag gtcaccgacg
      781 acggcgtggc gctcgtttcc ggccggctgc tggccgatat cgcgcgttcg ttgccgaaca
      841 agccggtcga catcaccgcc gacgagacca agatggaact ggtctgcggc accgcccggt
      901 tcaccctgca gacccttccg gtcgccgact acccggcgct gccggagatg cctgaagcca
      961 ccggcgcgat cttgagtgac gagttcgcca aggcggtggg ccaggtcgtg gtcgcagctg
     1021 gacgcgatga gttgctgccc gtgttcaccg gcgtccgcat ggagattgag ggcgagaccc
     1081 tctccctact ggccacagac cgctatcgca tggcgttgaa ggagctcgac tggaacccgc
     1141 gcagcagcgg cggtgagggt gcggcgctgg tgccggcccg cgtcctggcc gagaccgcga
     1201 agtcgatgac cgccggcgag caggtcacgc tgagcctctc gtccgccgcg accggcgacg
     1261 ggctgatcgg cttcgagggc accgggacgg ccggcgtccg ccagatcacc acccggctgc
     1321 tggacggcga gttcccgaag gtgcggcacc tgatgaacgt gcaggcagcg gtcagcgtcc
     1381 gcgccaacac cgcggaggtg atcgctgccg tgaagcgcgt gggcctggtg gctgagcgca
     1441 acacgtccct gcgcatgctg atcgcggagg gttcgatcac cctcgaggcg gccaccggcg
     1501 accaggcgca ggcagtagag gccctggagg ccgtggtcga agacgtcagc ggcaccgggc
     1561 ttgcgatgac tgctgccggc ttcaacccgc actacctgca ggacgcgctc tcggcgctgg
     1621 acagcccgta cgtgcagttc tcgttcaccc agcccggcaa gccctgcctg ctcaccggcc
     1681 tgaacgaact ggacggcgag ccgaatgccg actacaagca cgtcatcatg ctgatgcgcc
     1741 tgccggcgtg accttagcgt ccggtcaatc atccccgcag tctcaggtca gtcatcgccg
     1801 catgtctctg gttggtcatc ccggcgcagg ccgggatctc aaaaaccttg agcacatcgg
     1861 ctcctggggg tgatcgaaga tgttcgtcac ccatctgagc atcgctgact tccgttccta
     1921 ccagctggcc gacgtgccgc tcgagcccgg cgtgaccgtg ttgactggtg cgaacggcca
     1981 aggcaagaca aacctgatcg aagcggtcga gttcctggcg acgctcggat cgcaccgagt
     2041 ggcttccgag gcgccgctgg tccgagccgg ggccgagcgt tcgatcgtcc gggcgcgggt
     2101 gcaggccggc ttggacgatg cgcgcagcct gctgcttgag atcgagatca tccccggacg
     2161 ggccaacaag gcttcactta accgctcccc gctgcggcgg ccgcgcgatc tgctcggcgc
     2221 gctgcgggtg gtgctgttct cgccggagga cctggcgatc gtcaaggggg atccgagcga
     2281 gcgccgccgg ttcatcgacg agttactggt ggcgcgctgg ccgcggctgg ccggggtgcg
     2341 ggcggactac gaccgggtgc taaagcagcg ttccaccttg ttgaagtcac tttctgcacg
     2401 cggcccgcgg gcgctgaccg aagaggtcga gttcaccctt gaggtctggg acgggcaact
     2461 ggccgcgctc ggcgccgaga tcgttgcggc tcggctgacc acactggccg agctgggtcc
     2521 gttgctcgcg tccgcttatg ccgacatagc gccgacgaac aatttggccg cggtggcgta
     2581 cagatcgtcc gctctggacg ccgccgagcc gcgtgccgag gccactgctt ccgacaccat
     2641 tccaaccacc gccgaactga ccgagttgct caccgccaag atgaaggcgc gccgcaacga
     2701 ggagatcgct cgcggcctgt gcctggtcgg acctcatcgc gacgacctga cgctcacgct
     2761 cggcgacctg ccgacgaagg ggtacgcgtc ccacggcgag tcctggtcga ccgcgttggc
     2821 gctgcggttg gccagtttcg ggctgctgca ggcggacggg gttgtgccgg tgctgatcct
     2881 ggacgatgtg ttcgctgaac ttgacgaaac ccgtcgcgcc cggctggcgg aactgatcgc
     2941 cggcgccgag caggtgctga tcaccgctgc ggtggccgcc gacgtcccgt ctgcgctgca
     3001 gggcaggcgc ttcatcgtgg cgggcggcac cgtggttgca gaagccccgg cggctgacga
     3061 atgaccgaga ctcccgaaca cgacccgcag ggcaccgacc tggccaagca gattgcgcgc
     3121 gcggcccggg gcacgacccc gaagcctccg cagcgcaaac gccgtccggt ctcgcccacc
     3181 gacgagccca ctccgctagg cgacatcctc aaggaggtca tcgacgccca gggctggacg
     3241 cgcgaggtca acgtccacca actactggct cgctggcccg agctggtcgg agcggtgaac
     3301 gccgagcact cgaccccaac gggctatgcc gacaccgtgc tcacagtccg cgcggactcg
     3361 tccacctggg ccagctcgct gcgcctgatc gccccgcagt tggtggcccg gctgaacgaa
     3421 cagctcggcc agggcacggt cactagggtc aacgtcgtcg gccccgaggc gcccagctgg
     3481 aagaagggcc gtcgttctgt ccccggccgt ggcccgcgcg acacctatgg ctagccgctt
     3541 ccgacctacg aacgcggatt ggtctaggtt tcggggcccg gtctggattc cgaggttggc
     3601 ggggcggtgg ggctcggtca ggacgcggcg gcgcgtgcgg cttcggcctc gctgaacgcc
     3661 tcgcggacct cgtccaggat gccctgcatt gcgatccgcg cagcctccgg gtcgcccctg
     3721 aacacggcct cggctacagc ctcgtgtccg gcgagggctt cgggctttgg ctgggccggc
     3781 atcaggccga gctcggtgcg tccctgcagt acgaccgcga ccaggtcgct cagtgcggcg
     3841 aacagctcgt tgccgcacga cttcagcagc cggctgtgga actcgatgtc gaacttcatg
     3901 aactcgtgca gctgcccggc ttcgccggtg cgccgcatct ccgcggccag cggcagtagc
     3961 cgcgccctgg tgtggatgga cgcgaatcga gccgcgctct ccgcggccgc cggctcgacg
     4021 gcgaggcgta actgggtgag cgagtagatc tgttcttcgc gacgtgagga gtgcagtcgc
     4081 cagtcgatga gcagtgggtt gagcacgctc cactccgaca tcggtcgagc caccagcccg
     4141 aagcggcgcc gggtgcgcac cagacccatg gcctcaagct gacgggagac ctcgcgggcg
     4201 accgtccggg agacgccgaa acgcgactgc aggtcctcca gcgtccgcgc cgcttcgagg
     4261 ggccaggcac cgtcgatgat ctccagcccc agctcacgcg cgattgcgtg gtgcatgggt
     4321 gaggtggatt cggccgaatc ctcgctcatc aagcctccta gtcagcgtcg gggtaagcat
     4381 atccaaacca cggcacggac tacctttcag ggaagaaata atccgattct cttgattgag
     4441 agtacttatg taggttaagg tcatctcact gaccgcgccg ctgcgatcag tagaggagag
     4501 gtcaacgatg acgaacaccc atctggtgat catgggcgtg gcaggctgcg gcaagtcgtc
     4561 cgtcgccgaa gcgctgcaca aacagctcgg ctggatcgtg gcggaaggcg atgacttcca
     4621 cccccaggcc aacatcgcga agatgagctc gggcacgccg ctgaacgacg acgaccgctg
     4681 gccctggctg gatttgatcg ccgaatggac ggccacccag gacgcgtccg gacgatccac
     4741 gatcgtcacc tgctcagcac ttcgccgcgc ctaccgcgat cggctgcgca cagctcccgg
     4801 acggaccgtg ttcctgcacc tggtcggtac ccccgaacta ctcgccgaac gtctggccgg
     4861 ccgcaccgac cacttcatgc cagcgtcgct actgccgtcc cagctggcaa ctctagaagc
     4921 cctcgaagcc gacgaggcag gcttcgctgc cgacatcgac cgtccggtcg aagcgatcgc
     4981 caatgccgcg ctcgccgaac tccacctcaa cccgtccgac aagtcccacc actagacaac
     5041 ggagtcgtca atgaccacac cactaatcct ggccgctctc ggcggcatcg cgacgatcgt
     5101 cgtactgatc gtctggctga aagttcaccc cttcctctcg ctgatggccg gttccgccgt
     5161 gatggcgatc ttcgccggcg tcccctaccc cgacctcttc aagagcttca ccgctgggct
     5221 cgggtcgacg ctttccggcg tgggcctgct gatcatcctc ggcgcgatca tcggcaccct
     5281 gctggtctcc tccggcggcg cggacgtgat cgtcgacacg atcctcgcga aaaccccgct
     5341 gcaacggctg ccgtgggcga tggcgttgat cgccttcgtc gtcggcattc cgctgttctt
     5401 cgaggtcggc gtggtcatcc tgatcccggt cgtgatgttt gccgctcgcc gtgccaacct
     5461 tccggtggtg ctgctgggca tcccggcact ggccggcctg tcggccctgc acgggctggt
     5521 gccgccgcac cccggcccgc tgatcgcgat cgacgccctc ggcgccaacc tcggcctgac
     5581 cctcggtctc ggcctgctcg tggcaatccc atgtgtgatc atcgccggcc cgctgctcgg
     5641 caaggtgatg gcgaaatggg tgccgatcga ggcgcgctcc gacctgctgg gctccggcgg
     5701 accggcggaa ggtgccaagc ggcccagctt cggcgccgcg ctgaccgtcg tcctgctgcc
     5761 ggtgatcctg atgctggcgc gcaccgtggt ggaaatgacc ggcgccgagg agaacccgtt
     5821 cggtgccgcc ctggttttcg tcggcacccc gctgatcgcg ttgacgatca ccaccatcgc
     5881 ggccatgttc atgctcggct tcctgctctc gaagactcgg gacgaagtga gcaaactggt
     5941 cggctcggcc ttcggtccga tcgctggcat cctgctgatc gtcggcgccg gcggtggctt
     6001 caagcagacc ctggtcgact ccggcatcgc ggacgtgatc gccaagggcc tggcggacgc
     6061 ggccctgccg ccgctgctgg ctggctggat cgtggccgtg ctgatccgcc tcgccaccgg
     6121 ttccgcgacc gtcgccacca tcaccgcgtc cggcatcatg gcgccgctgg ccgccggcct
     6181 gccgccgacg cacgccgcct tgatggtgct ggcgatcggg gccggctcgg tgttcctcag
     6241 ccacgtcaac gacgccggct tctggatggt gaaggagtac ttcggcatgt cggtcggcca
     6301 gaccttcaag acctggtcgc tgatggagac cgtgctgtcg gtgaccggcc tggccggcgt
     6361 gatgctgctt agcctggtgg tctgatgtcc cccagtcctg caccggctgt cgtcgccgag
     6421 gtgaccgagc gaatccggcg gcgcagccga gccggacggc gggcctacct cgatcggatc
     6481 cacgcggacg ccgagcgggc cgactcccgg acgcggctga gctgcgcgaa cctcgcgcac
     6541 gctttcgccg cctccccgga atcggagcgg ctcgccgtgc tcaaggccgc ccggccgaat
     6601 ctcgggatcg tcaccgctta caacgacgtc ctctccgcgc accgtccgta cgaggactat
     6661 ccggccgtga tccgggacga ggcccgccgc ggcggcgcct cggcccaggt cgccggcggc
     6721 accccggcga tgtgcgacgg ggtgacccag ggcactgtgg gtatggagtt gtcgttgttc
     6781 agccgggacg tgatcgcgat gagcacggcg atcgcgctca gccacgacgt gtacgacgcg
     6841 gccctgctga tgggcatctg cgacaagatc gtgccgggaa tgctgatcgg cgccctctcc
     6901 gcgggccacg tcccctgtgt cttcgtcccc gccgggccga tggggagcgg catcgcgaac
     6961 gcggccaagg ccaaagtgcg cgaggcccac gtccggggcg acgcgtcccg cgacgagcta
     7021 ttggcggtcg agcaacaggc ataccactcc gccggcacct gcaccttcta cggcaccgcg
     7081 aactcgaacc aggtgctgct ggaggtcatg ggcctgcacg tgcccggcgc ggcgttcgtg
     7141 ccgccgtact cgccgctgcg ggatgcactc acccggcagg ccgtgcggac ggcgttgcag
     7201 gtgcgggccg gtggtgaccg gttcactccg atcggggagt tggtggacga gcggtcgatc
     7261 gtgaacgcac tggtcacact gctggccacc ggcggctcga ccaaccacct gattcactgg
     7321 gtggccgtag cgcgtgcggc cggcatcgtc ttcgactgga gcgatgtcga ccaattgtcg
     7381 ggcgtggtac cgctgctggc gcggatctat ccgaacggcc aggccgatat caacgacttc
     7441 gagcgcgccg ggggcaccgg cttcgtgctg cgcgagttgc tcgaccttgg cctcctgcac
     7501 gcggacgtgc cgaccgtcaa cgccggcggg ctggccgcgc aggcgctgcg tccggaactt
     7561 gtcgagggcg ggttgcggtt cgccgcgcca ccagcggaaa caccggacgc tgcggtgctg
     7621 cgcggtggcg ccgacccgtt cgacaccacc ggcggcctgg cactaatcgc cggcaatctg
     7681 ggccgcgcgg tgatcaaggt cgccgcggtg gcgcccgagc accgggtgat cgaggcacca
     7741 gccttcgtcg ccgattccca ggacgaggtg gcggccgcct tccagcgcgg cgatctcgac
     7801 cgcgatgtct tggtggtcgt ccgcttccag gggccgcgcg cgaacggcat gcccgaacta
     7861 caccggctca ccgcgatcct cggcgtcctg cagtccgccg ggcatcgggt cgccctggtg
     7921 accgacggac ggatgtccgg cgcttccgga cggatcccgg cagctatcca cctgaccccc
     7981 gaagccgcgg tcggtgggcc gtgcgcgaag ctgcgcaccg gggacctgat ccggctggac
     8041 gcgaacgcag gcaccatcga tgtgctggcc gtagaccttg agcaacgtac gcgggcacgc
     8101 tgggacgggc cgcatccgct gggccttggt cgcgacctgt tcgccggcat gcgggccagc
     8161 gtgaccggcg ccgaacaggg cgcctgcagc tggctgccct tcaccgacga tctcgaccca
     8221 gccgctgcag acaacacgac ctaccgacgg gatttggtga cgcaatgacg catgaaccct
     8281 ggtccggaca tggaccgatc attccggtgc tggtgatcga ggacgccggc atcgccgtcg
     8341 acctggctcg agcgctggtc gcgggcggcc tgtcagcgct cgaggtgacc ctgcggacgc
     8401 cggccgctcc gcacgccatc gcagccatcg ccgcggaggt gccagaggcc accgtgggcg
     8461 ccggtaccct ccggctgccg gccgacgcgg cgctggccaa ggacgcgggc gccaccttcg
     8521 cggtctcgcc gggctacacc caggacttgg gcgccgcctg ccgggaagcg ggtctgccgc
     8581 tgctgcccgg cgttgcgacc gcgtccgaat tgttgctggc ctacgcggac ggccaccggt
     8641 tctgcaagtt ctttcccgcg gtggctgcgg gcggcgtccc ggttctgaaa gccctagccg
     8701 gaccatttcc ggacgtatcg ttctgcccga ccggcggcat caccgaagcc accgcaccgg
     8761 acttcctcgc gctcgctact gtgccagtct gtggcggcac ttggctgacg cctgctgaac
     8821 tggttaccac ccgtgactgg acgggtatca ccgaactggc ccggcgcgcc gcggcgctcg
     8881 gacgagaggg gacatcatgc agctaggaat gatcggcctc ggcaagatgg gccacaacat
     8941 ggcggaacgc cttcgccgtg ccggccacga ggtgatcggc tacgaccgta atccggcggt
     9001 ctccgagacc accgacctgg ccgagctggt cgaccggctg gccggtccgc gggtgttgtg
     9061 gttgatggtc cctgccggtg cgcccaccca ggccaccgtc gccgaacttg ctgagttgct
     9121 cggagccggg gacgtggtca tcgacggcgg caactcctac tacaaggacg acgcgatcaa
     9181 ctccgccctg ctggccgagc atggcgtcgg ctacctcgac tgtggggtgt ccggcggcgt
     9241 gtggggtctt gagaacggct acggcctgat ggtcggcggt gaagcgggcg aggtggccag
     9301 ggtgatgccg atcttcgacg cgctgcgtcc ggacggcccg cgggacgagg gtttcgtcca
     9361 cgctggcgcg gtgggcgccg ggcactacgc caagatggtg cacaacggca tcgagtacgg
     9421 cctgatgcat gcctacgccg agggctggga gctgctggag acaaatggca cggtgaccga
     9481 cgtggccggc tgcttcaagg cgtggacgcg gggcacggtc gtccgctcct ggctgctcga
     9541 cctgctggtc aaggcgcttg aggagaaccc gggcctggcc gacgtggacg agtacaccac
     9601 tgattcgggc gagggccggt ggacgctgca ggaggcgatc gacaacgcgg tgccgatgcc
     9661 ggtgctggcc gcttccctgt tcgcccggtt ctcgtccagg cagccgaaca gcccgagcat
     9721 gcagatggtg tcggcgctgc gcggccagtt tggcggtcac gcggtggtgc acacgccgcc
     9781 accggcctga tccactggac ccgcacggac gcggaccccg tccgccgtcc agcccgtcca
     9841 ctccaacttc gctcccattc gggagcgaag ttgcgttcgg cggcgacgag cagccagttc
     9901 gggccgattt gaccgcctgc ggggtctgga cgcgtacccg tccttgcgtg ggcccctgat
     9961 cggggctcgg aaacgtcacc gctggccgca cagggccaaa tgctgagcgg gcgtgccccg
    10021 aattctggcc ggaatccgct agactcggtt cgttccggag cgcgccgcca gcatcctcgg
    10081 cggcgcttgc ccatgtctga agggaaagcc tgcgtgactg acgagccgat cgtcgctgcc
    10141 ataaccgctg cggaagccgc acacgtagcc gaaggcagct acgacgcgtc cgcgatcact
    10201 gtcctcgaag gcctggacgc ggtacgcaag cgtccgggca tgtacatcgg ctcgaccggt
    10261 gagcggggcc tgcaccacct ggtctacgag gtggtggaca acgcggtgga cgaggcactg
    10321 gccggctact gcgacaacat catcgtgcgt ctgctggacg gtggcggcat ccaggtggtc
    10381 gacaacggcc gcggcatccc ggtcaaggaa cacccaaagg agaagatctc cgcgctaacc
    10441 gtcgcgctga cgatcctgca cgccggcggc aagttcggcg acggtggcta caaggtgtcc
    10501 ggcgggctgc acggcgtggg tgtctcggtg gtgaacgcgc tgagctccaa gctcaccgtc
    10561 gacgtcaatc gcgacggcta ccactggcgc cagtccttca gcctcggcga tccggacgcg
    10621 ccgctggagc agctggagcc caccgaggag accggcacga cggtcacctt ctacgccagc
    10681 ccggagatct tcgagaccac caactactcc tacgagaccc tggccacccg cttccgcgag
    10741 atggcgttcc tcaacaaggg cctcaagatc aacatcgtcg acgagcggac ccaggagtcc
    10801 gacaccgacg acgatgacaa cgacgacgtc gagcagcgct cacagacctt ccagtacacc
    10861 gacggcctga tcgactacgt gaagtacctc accggcagca aggacacgat ccaccccagc
    10921 gtgatcgcga tcgagcaggc cgccgaggag cagcggatga gcctcgaagt ggcgatgcag
    10981 tggaacacgt ccttcaacga gagcgtgcac accttcgcca acacgatcaa cacccacgag
    11041 ggcggcaccc acgaggaggg cttccgcgcc gcgctgacca
//
LOCUS       DAOPRQ010000074        10973 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_5019_length_10973_cov_12.269830, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000074 DAOPRQ010000000
VERSION     DAOPRQ010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 10973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10973
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5019_length_10973_cov_12.269830"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="PKJ61_09635"
     CDS             complement(<1..269)
                     /locus_tag="PKJ61_09635"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HOC14125.1"
                     /translation="MFGQVKYEAMNAHLNSMLERRRVLISAHRGSGGGSIAVNTELGI
                     RAAFLSGADLVEIDVSASVDGEFFCFHDGCEPELLGIEANLQTLTA"
     gene            416..2080
                     /locus_tag="PKJ61_09640"
     CDS             416..2080
                     /locus_tag="PKJ61_09640"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019047543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HOC14126.1"
                     /translation="MTEFRYADLLPIGADATPYRLLTTEGVETVEGPDGRSFLKVAPE
                     ALELLAETAMHDIAHYLRPAHLQQLRNILDDPEASGNDKFVALDLMKNANIAAGGVLP
                     MCQDTGTAIIMGKRGQQVLTDGTDELPLSRGVFEAYTKLNLRYSMNAPITMWEEKNTG
                     NNLPAQIELYADTASGHENTYKFLFMAKGGGSANKSYLYQETKAILNPASMMKFLDEK
                     LRSLGTAACPPYHLAIVIGGTSAEYALKTAKYASAKYLDSLPTSGSMDAHGFRDLELE
                     EQVLQLTRDFGIGAQFGGKYFCHDVRVIRLPRHGASLPVAISVSCSADRQCMGKITPE
                     GVFIEQLEREPAHFMPDTTDEHLEDSAVVEIDLGRPMPEILAELSKYPVKTRLSLHGT
                     VIVGRDIAHAKIKERLDAGEPMPQYLKDHPVYYAGPAKTPEGMPSGSFGPTTAGRMDS
                     YVDQFQAAGGSMIMLAKGNRSKAVTDACAAHGGFYLGSIGGPAARLAQDCITSVELLE
                     YPELGMEAIWKIEVKDFPAFIVVDDKGNDFFAEVNRPTLIGLSPRS"
     gene            complement(2220..3506)
                     /locus_tag="PKJ61_09645"
     CDS             complement(2220..3506)
                     /locus_tag="PKJ61_09645"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020580257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HOC14127.1"
                     /translation="MSNATFSREGVSLELPLAEATEGNDGYDITKLLAATGTTTLDVG
                     FANTASCRSAITFIDGDAGILRYRGYPIEQLAEHSSFLEVSYLVLHGELPTADQLATF
                     SSNIAKYHLIDERMREMFRSLPRKSHPMPVLAAGLNALSTFTASKYGEDSLHDLDRAT
                     HLLMGSLPTLAAYGYKTSVGQPMLYPDDSLSYIENFLRLSFGVPTGPYPFDDAITRAL
                     DVLLILHADHEQNCSTSTVRMVGSSGANIYVSVAAGVSALSGPLHGGANQAVLEMLKG
                     IRDEGGDVKSYVAKVKDKKDTTKLMGFGHRIYKNYDPRAAIVKKHADAILNTLSGHDQ
                     LLDLAVQLEEAALSDSYFQERKLYPNVDFYTGLIYQAMGFPQNMFTVLFALGRLPGWI
                     SHFREQAGDPATKINRPRQIYIGEVERPYVPMEQRG"
     gene            3690..4235
                     /locus_tag="PKJ61_09650"
     CDS             3690..4235
                     /locus_tag="PKJ61_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012922161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HOC14128.1"
                     /translation="MTSIRDLSGTYNLDPSHSRVGFVARHAMIAKVRGAFNEVAGTAT
                     IDGANPSASALDVTIQTASVDTRDANRDSHLASPDFFDAAQYPTITFKATDFAVTDDQ
                     TVEVTGDLTIKDITRPVVVPFTFEGVATDPFGNERAGFEGQVTINRKDWGLTWNAALE
                     TGGVLVGDKVTLELEVSAVKA"
     gene            complement(4316..5545)
                     /gene="rocD"
                     /locus_tag="PKJ61_09655"
     CDS             complement(4316..5545)
                     /gene="rocD"
                     /locus_tag="PKJ61_09655"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HOC14129.1"
                     /translation="MHALTEISDQQQVIEQIETYSPHNYHPLPVVIESGSGAWLVDTE
                     GKRYLDFLSAYSAVNFGHSNPELLAVASEQLAKLSITSRAVFSAPFGPFVEGLAKLCG
                     KDMVLPMNTGVEAVESSLKLARKWGYEVKGVPEDLANIIVMEGNFHGRTISVISFSSD
                     SDATDSYGPFTPGFVKVPYGDLDAIEAAIDEHTVAILVEPIQGEAGVVTPPSDFLPGI
                     RSLADRHQVLMIADEIQSGLGRTGSTFYCEQVGVVPDLYLLGKALGGGIVPVSAVVGN
                     RDVLGLLKPGQHGSTFGGNPLACAVGLAVVRLLETGEMQARSRELGDYLHSRLLELLG
                     RGVTEVRGIGLWAGVDIDPTLGTARDICERLLAKGVIAKDTHGSTIRLAPPLVITREE
                     LDLALDALASALAEASA"
     gene            complement(5602..6915)
                     /locus_tag="PKJ61_09660"
     CDS             complement(5602..6915)
                     /locus_tag="PKJ61_09660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14130.1"
                     /translation="MIPRRTLILGSAGRDFHVFATVFRSDPRHEVLGFTSSALSAGER
                     GVFPSCLCGDSYPAGIPIIAEADLERFVASERIEKVVFAYAEIRCTGVAAVAARVLAA
                     GADFQLLSPNRSMLTAQKPVIAVSSAHNGAGKTPTVRHLTDLLLTQGVRVAVLRHPVQ
                     RSGFTAGWQHQFSDDEPGLDACARPQSRAIGDQPGAIRVDGLDHAAVLAAAEEQADVI
                     LWDSAGTDLPFVRADLHLVLVDPLRADAGLDCFPGEVTLRLADAVIISKCDAVSDELV
                     QQSHRQITRLNPGATVFTADSPVVIDHPERIAGRTVVVVEEEPTLAFGDLRPGAGIAA
                     AHAVGVSAIISPLPQAVGSLVAVYACHPEAQSVLPVADREPATLADVRATAEATPSEA
                     VIDATTLGLDGLFDLGRPVAKAGYALRPHDPDALAALVLERVRPR"
     gene            7170..7667
                     /locus_tag="PKJ61_09665"
     CDS             7170..7667
                     /locus_tag="PKJ61_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007077479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOC14131.1"
                     /translation="MEQQAPIVIGYDGSEFAAEALRKGLWLAGKVGAGVRIVRAWTIS
                     NAPRPKSWSPGYVPPVEDFAEAVLAELTKATAAIVAEFPEVEVSFEAPHGAAGRELIK
                     ASADAQLVVVGTRGLGGFTGLLIGSVSDQVVEHAGCDVLVVRRRSGDQAPPRTLKLDR
                     ALDSE"
     gene            7719..8651
                     /locus_tag="PKJ61_09670"
     CDS             7719..8651
                     /locus_tag="PKJ61_09670"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOC14132.1"
                     /translation="MKSAGRAALAATVVALLFSGCATVPPRTLAARAAESIPPSQVNA
                     VVGFPLPTRPVASPLVAGSDAAAPVDSAATDPSAVPSPAEPTGPATEPPAEPPAPEPE
                     PPAEPAPVDCAVLKCVALTMDDGPVPGTGKLLDLFARRGVKATFFLLGQNAKAHPKLV
                     RRMVAEGHELGNHSWSHPEFWNLSKSGIRKQLVKTNKLLTKLGQRPKLVRPPYGEWDR
                     DVAKVTRSLGMPAVIWDVDPEDWKVRKAKTVAKRVLKQVERGSIILTHDSLKSTRKAI
                     PAIVRGLQAKGYTLVTVSQLMAGKLHPGKRCFHA"
     gene            complement(8668..10023)
                     /locus_tag="PKJ61_09675"
     CDS             complement(8668..10023)
                     /locus_tag="PKJ61_09675"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HOC14133.1"
                     /translation="MAEPRLLWFRRDLRLGDHPALAAAAAAGPVVPLFVLDPAFFDNA
                     GAVRQGWLAANLVALREALDSRLCLRWGDPVRVVPELAAEVGAVVVHVSTETEPGGLA
                     RDQRVQRALAAAGVEWVETGSPYAVTPGRVRTLSGSGFQVFTAFHRAWQAHGWRGPAL
                     LPDAFTPMRAAGNPAAWGRIEAALAACPIALPDAGEAAALGRWRRFLDADLSGYSGDR
                     DRPDLDTTSRLSPDLKFGVVHPRTLLADLAERPGPDADRYATELAWREFYADVLSRHP
                     ESLAADLKSIKLDYDEPSAGFEAWRLGRTGFPIVDAGMRQLLAEGWMHNRVRMITASF
                     LTKDLHHWWPLGATHFLDHLIDGDLASNAHGWQWTAGTGTDAAPYFRVFNPTSQGLRF
                     DPNGDYVRRWVPELRHLRGATAHEPSKAADGYRHGYPRPILDHGRERLEALARYRAAR
                     G"
     gene            complement(10016..10891)
                     /locus_tag="PKJ61_09680"
     CDS             complement(10016..10891)
                     /locus_tag="PKJ61_09680"
                     /inference="COORDINATES: protein motif:HMM:NF014646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOC14134.1"
                     /translation="MPDTVIPETTTLLTIKAAAEVTGLSEHTLRAWERRYELLAPQRT
                     ASGYRLYDRQTLARVRAMQELVQAGWSPKSAAAELLRRPPPLGDVTDSSAELIEAAAA
                     MDAASIHRIVTESFGAAAFETVVDDWMLPALARLGQAWADGKVTVAGEHLVADALMRR
                     LAAAYDSVRRGQGGPPVLIGAPPGVDHQLGLLAFAVACRRAGLATVYLGAKVPLPAWR
                     DAALRTRAFAVVTSVPQRRDSERVTRVAAQLESLPEVSVWVGGRFQHLVPAPARTLGH
                     LIGPAARTLAASRHG"
BASE COUNT         1844 a         3658 c         3787 g         1684 t
ORIGIN      
        1 gcggtgaggg tctgcaggtt ggcctcgatg ccgagcagct cgggttcgca gccgtcgtgg
       61 aagcagaaga actccccgtc cacggacgcg gagacgtcga tctccaccag gtcggcgccg
      121 gacaggaaag ccgccctgat gccaagttcg gtgttgaccg cgatgctgcc gccgccactg
      181 ccgcggtgag ccgagatcag cactcggcga cgctccaaca tcgagttcag gtgggcgttc
      241 atggcctcgt acttcacttg gccgaacatc gaacctccgc ggggtgtggg gtgtgcgaac
      301 gacattgtca gcacacattc gactctaccc acgaccacga ccggataacc aagtctggca
      361 atagtcgcga tcccgctgtg ggacgatttt gtacgggcta gagcgaagga gtgtcatgac
      421 cgaattccgc tatgcggatc tgttgccgat cggggcggac gccaccccct accggctcct
      481 caccaccgag ggtgtggaga cggtcgaggg gccggacgga cggtctttcc taaaggtggc
      541 gcccgaggcg ctggagttgt tggccgagac cgcgatgcac gacatcgccc actacctgcg
      601 gccggcgcac ctgcagcagc tgcgcaacat cctcgacgac ccggaggcca gcggcaacga
      661 caagttcgtc gcgctggacc tgatgaagaa cgccaacatc gctgccggtg gcgtgctgcc
      721 gatgtgccag gacaccggca ccgcgatcat catgggcaag cgtggccagc aggtgctcac
      781 cgacggcacc gacgaactgc cgctctcgcg cggcgtcttc gaggcgtaca ccaagctgaa
      841 cctgcgctac tcgatgaacg cgccgatcac catgtgggaa gagaagaaca ccggcaacaa
      901 cctgccggcg cagatcgagc tgtacgccga caccgcgtcc ggccacgaga acacctacaa
      961 gttcctgttc atggcgaagg gcggcggctc ggccaacaag agctacctct accaggagac
     1021 caaggcgatc ctgaatcccg cgtccatgat gaagttcctg gacgagaagc tgcgcagcct
     1081 cggcaccgcg gcctgcccgc cgtaccacct ggcgatcgtg atcggcggca cgtcggctga
     1141 gtacgcgctg aagacggcca agtatgcgtc cgcgaagtac ctggactcgc tgccgacttc
     1201 gggctcgatg gacgcccacg gcttccgcga cctggagctc gaggagcagg tgctgcagct
     1261 gacccgcgat ttcggcatcg gcgcgcagtt cggcggaaag tacttctgcc acgacgtccg
     1321 ggtgatccgg ctgccgcggc acggcgcgtc cctgccggtg gcgatctcgg tctcctgctc
     1381 ggccgaccgg cagtgcatgg gcaagatcac gcctgaaggt gtgttcatcg agcagttgga
     1441 gcgcgagcca gcgcacttca tgcccgacac gaccgacgag caccttgaag acagcgcggt
     1501 cgtcgagatc gacctcggcc ggccgatgcc ggagatcctc gccgagctga gtaagtaccc
     1561 ggtgaagacc cggctttcgc tgcacggcac ggtgatcgtc ggacgcgaca tcgcgcacgc
     1621 caagatcaag gagcgcctgg acgcgggcga gccgatgccg cagtacctga aggaccaccc
     1681 ggtctactac gccgggccgg cgaagacgcc cgaggggatg ccgtccggct cgttcgggcc
     1741 gacaaccgcc ggacggatgg attcctacgt cgaccagttc caggctgccg gaggctcgat
     1801 gatcatgctg gcgaagggga accgctcgaa ggctgtgacc gacgcctgcg ctgcgcacgg
     1861 tggcttctac ctcggctcga tcggtggccc ggctgcccgg ctggcgcagg actgcatcac
     1921 cagcgtcgag ctgctcgagt acccggaact gggcatggag gcgatctgga agatcgaggt
     1981 gaaggacttc ccggcgttca tcgtggtgga cgacaagggc aacgacttct tcgccgaagt
     2041 gaaccgtccg acgctgatcg ggttgtcccc gcgcagctga gatcagccgt cgctggggcc
     2101 caccaatcgg ggttgaaagc accgccaacg gcaacttcgc tcccgaaagg gactcccgct
     2161 cccgaatttt cgggagcaag cgtcactttc gggagcgaag ttcgctgggg gtagacccgc
     2221 tacccccgct gctccatcgg gacgtaggga cgctcgacct caccgatgta gatctgccgc
     2281 ggccggttga tcttggtggc ggggtcgccg gcctgctcgc ggaagtgcga gatccaaccg
     2341 ggcagccggc ccagggcgaa gagcaccgtg aacatgttct gcgggaagcc catcgcctgg
     2401 tagatcaggc cggtgtagaa gtcgacgttc gggtagagct tgcgctcctg gaagtacgaa
     2461 tcggacagcg ccgcctcttc cagctgcacc gcgaggtcga gcagctgatc gtggcccgac
     2521 aaggtgttca ggatcgcgtc cgcatgcttc ttcacgatcg ccgcgcgcgg atcgtagttc
     2581 ttgtagatgc ggtggccgaa gcccatcagc ttggtggtgt ccttcttgtc cttcaccttg
     2641 gcgacgtagc tcttcacgtc gccgccctcg tcgcgaatgc ccttgagcat ctcgagcacg
     2701 gcctggttgg cgccgccgtg cagcgggccg gatagggcgc tcaccccggc cgcgaccgag
     2761 acgtaaatgt tggcgccgga cgatccgacc atccggacgg tcgacgtcga acagttctgc
     2821 tcgtggtcgg cgtgcaggat cagcagcacg tcgagcgcgc gggtgatcgc atcgtcgaag
     2881 gggtagggcc cggtcggcac gccgaacgag agccgcagga agttctcgat gtagctcagc
     2941 gaatcgtcgg ggtagagcat cggctggccg accgaggtct tgtagccgta ggcggccagg
     3001 gtgggcagcg agcccatcag caggtgggtg gcgcgatcga ggtcgtgcag cgaatcctcg
     3061 ccgtacttgg acgcggtgaa ggtcgacagc gcattcagtc cggcggccag caccggcatc
     3121 gggtgcgact tgcgcggcag cgagcggaac atctcgcgca tccgctcgtc gatcaggtgg
     3181 tacttggcga tgttggacga gaaggtggcc agctggtcag cggtgggcag ctcgccgtgg
     3241 agcaccagat aggagacctc caggaagctg gagtgctcgg ccagttgctc gatcgggtag
     3301 ccgcgatacc tcaggatgcc cgcgtcaccg tcgatgaagg tgatcgcgga acggcaggac
     3361 gccgtgttgg cgaagccgac gtccagggtg gtggttccgg tggccgccag caatttggtg
     3421 atgtcgtagc cgtcgttacc ctcggtggct tccgccaagg ggagttcgag ggatacccct
     3481 tcgcggctga aggtcgcgtt gctcatccat cctccgtctc gtcggacgcg cggcagcgcg
     3541 cgtccggctc agtaagtggt gtcacaatac cccgccccac ccacccgcgg cagggcttcg
     3601 gcgtaccctc ccaggaggtg aagtagttga cggatcaatc accgtcgact atggttttac
     3661 ttgagacttc aatcaaagga gagcacgaca tgacttcgat ccgggacctg tccggcacct
     3721 acaacctcga cccctcgcac tcgcgagtcg gcttcgtggc tcgccacgcg atgatcgcca
     3781 aggtgcgggg ggcgttcaac gaggtcgccg ggaccgcgac catcgacggc gccaacccgt
     3841 ccgcttccgc tctggacgtg accatccaga ccgccagcgt cgacacccgg gacgccaacc
     3901 gcgacagcca cctggccagc ccggacttct tcgacgcggc ccagtacccg acgatcacct
     3961 tcaaggccac cgatttcgcg gtgaccgacg accagaccgt cgaggtgacc ggcgacctga
     4021 cgatcaagga catcaccagg ccggtcgttg tgccgttcac gttcgagggc gtggccaccg
     4081 acccgttcgg caacgagcgc gccggcttcg agggccaggt caccatcaac cgcaaggact
     4141 ggggcctcac ctggaacgcg gcgctggaga ccggcggcgt cctggtcggc gacaaggtca
     4201 ccctcgagct cgaggtttcc gccgtcaagg cctgaacaag aacggagacg gttcctttac
     4261 tggttcacac cgaacgctcg gtgtgaacca aaaaagggac cgtccccttt actgttcagg
     4321 cggacgcttc ggcgagcgcc gacgcgagcg cgtccagggc caggtcgagt tcctctcggg
     4381 tgatcaccag gggcggggcc aaccgtatgg tcgagccgtg ggtgtccttg gcgatcacgc
     4441 ccttggccag caggcgctcg cagatgtcgc gggccgtgcc caaggtgggg tcgatgtcga
     4501 cgccggccca caggccgatc ccgcgcacct cggtgacccc gcgtccgagc agttcgagaa
     4561 ggcgggagtg caggtagtcg ccgagttcgc gggagcgggc ctgcatctcg ccggtctcca
     4621 gcagccgcac cacggccagg cccaccgcgc aggccagtgg gttgccgccg aaagtcgagc
     4681 cgtgctgacc cggcttgagc aggccaagaa cgtcccgatt gccgaccacg gccgagaccg
     4741 gcacgatgcc gccgccaagc gccttgccga gcaggtacag gtcgggcacc acgccgacct
     4801 gctcgcaata gaaggtgctc ccggtgcgac cgagtcccga ctggatctcg tcggcgatca
     4861 tcagcacctg gtggcgatcg gcgagtgagc gaatgccggg caggaagtcg gacggcgggg
     4921 tgaccacgcc ggcctcaccc tggatcggct cgaccaggat cgccacggtg tgctcgtcga
     4981 tggccgcctc gatcgcgtcc aggtcgccgt agggaacctt cacgaatccc ggggtgaacg
     5041 ggccgtagga gtcggtggcg tcggagtcgg acgagaacga gatcacgctg atcgtgcgtc
     5101 cgtggaaatt cccctccatc acgatgatgt tcgccagatc ctcgggcacg cccttgacct
     5161 cgtagcccca cttgcgggcc agcttcaaag aggactccac cgcctcgacg ccggtgttca
     5221 tcggcagcac catgtccttg ccacacagct tggcaaggcc ttccacgaag ggtccgaagg
     5281 gtgcagagaa gacggcgcgc gaggtgatcg acagcttcgc caactgctcg gacgcgaccg
     5341 cgagcagctc gggattggaa tgaccgaaat tcaccgctga gtaggcggac aagaagtcga
     5401 ggtagcgctt gccctcggtg tcgaccagcc acgcacccga ccccgactcg atcacgaccg
     5461 gtagcggatg gtagttgtgc gggctgtagg tctcgatctg ctcgatgacc tgctgctggt
     5521 ccgaaatctc ggtgagcgcg tgcatggcgt cctttctgcc gaccagtgat gctaccggtt
     5581 cagcgccgat gcccctttcg ctcagcgcgg acggacccgc tccagcacca gcgccgccag
     5641 agcgtcaggg tcgtgcggac gcagcgcgta cccggccttc gccaccggac ggcccaggtc
     5701 gaacaacccg tccaggccga gcgtggtggc gtcgatgacc gcctcgctcg gggtcgcttc
     5761 ggcggtggcc cggacgtcgg ccagcgttgc cggttcccga tcggccaccg gcagcaccga
     5821 ctgggcttcg ggatggcagg cgtagaccgc gaccagggac ccgaccgcct gcggcagcgg
     5881 gctgatgatc gccgagaccc cgaccgcatg cgcggccgcg atgcccgcac ccgggcgcag
     5941 gtcgccgaag gcgagcgtcg gctcctcctc gaccaccacc acggtgcggc cggcgatgcg
     6001 ttcgggatga tcgatcacca ccggcgaatc ggcggtgaac acggtggccc cggggttgag
     6061 ccgggtgatc tggcgatgcg actgctgcac cagctcgtcg ctgacggcgt cgcacttgct
     6121 gatgatcacc gcgtccgcca gtcgcagggt gacctcgccg gggaagcagt ccaggccggc
     6181 gtcggcccgc agcggatcga ccagcaccag atgcagatcg gcccgcacga agggcaggtc
     6241 ggtgcccgcg ctgtcccaga ggatcacgtc ggcctgttcc tcggctgcgg ccaggacggc
     6301 agcgtggtcg agcccgtcca cccggatcgc gccgggctgg tcgccgatgg cccggctctg
     6361 cggacgtgcg caggcgtcga ggcccggttc gtcgtcgctg aactggtgct gccacccagc
     6421 ggtgaacccc gaacgctgga ccggatgccg caacacggcc acccggacgc cctgggtcag
     6481 cagcaggtcg gtcaagtgcc gcacggtggg ggtcttgccg gccccgttgt gggccgaact
     6541 gaccgcgatc accggctttt gagcggttag catcgagcgg ttcgggctga gcagctggaa
     6601 gtcggccccg gcggccagca cccgcgcggc gaccgcggcc acgccggtgc accggatctc
     6661 ggcataggcg aagacgacct tctcgatccg ctcggacgcg acgaaccgct ccaggtcggc
     6721 ctcggcgatg atcgggatac cggccgggta ggagtccccg cacaagcagg acggaaagac
     6781 cccacgttca ccggccgaca gtgccgaact ggtgaatccc aacacctcgt ggcgaggatc
     6841 tgagcggaaa acggtggcga agacgtggaa atcacgcccg gctgaaccga ggatcaatgt
     6901 gcggcgcggg atcatggcgg cggctccttt ctcccacggc tgagtatacg gcggcggccg
     6961 tcggtggtcg gccagtcggc tctatcccga gaacccgtca cccactcgcg atgcgccgtc
     7021 cgccgattcc gatgagctga ttcccgccct ggttaccgaa cgacagccgc tttcgggccc
     7081 gcgcggccag ccagggcatg gatcgcctca acgtccccag gggcgtcgcg ggtatcgtcg
     7141 gcgaacgagg tcgacggtag agtcgggcca tggagcaaca ggcgccgatc gttatcggct
     7201 acgacggttc ggagttcgcg gcggaagcgc tgcgcaaggg cttgtggctg gctggaaagg
     7261 tcggggccgg cgtccggata gtgcgggcct ggacgatcag caacgcgccg cgtccgaagt
     7321 cgtggagccc gggttatgtg cctccggtgg aggacttcgc cgaggcggtc ctcgccgaac
     7381 tgaccaaggc gacggcagcc atcgtcgctg agttccctga ggttgaggtg agcttcgaag
     7441 cgccccacgg agcggccggg cgcgaactca tcaaggcttc cgccgacgcc cagctggtgg
     7501 tcgtgggtac ccgcgggctc ggcggtttca ccggcttgct gatcggctcg gtctccgacc
     7561 aggtggtcga gcatgccggt tgtgacgtcc tggtggttcg ccgacgctcc ggcgaccagg
     7621 cgccgccgcg gaccctgaag ctggatcgcg cgctggacag cgagtgaacc ccgctccggc
     7681 ggtgcggtac ggagaacgac gcctactagg ctgaagttgt gaagtctgcg gggcgggcgg
     7741 ccctggccgc gacggtggtt gcgctgctgt tcagcggctg cgcgaccgtg ccgccgcgaa
     7801 ccctggctgc ccgggccgcc gaatcgattc cgccgtcgca ggtcaatgcc gtggtcgggt
     7861 tcccgctacc gacacgcccg gtcgcgtccc cgttggtagc cggctcggac gccgccgcgc
     7921 cggttgactc ggcagcgacc gatccgtccg cggtcccgtc cccggccgag ccgacggggc
     7981 cggcaaccga accgccggcg gagcccccgg cgcctgagcc cgagccgccc gcggaaccgg
     8041 ctccggtgga ttgcgcggtg ctgaagtgcg ttgccctgac catggatgac ggaccggtgc
     8101 caggcacagg gaagttgctc gatctctttg ccaggcgtgg cgtgaaggcg accttcttcc
     8161 tactcggcca gaacgcgaag gcgcacccca aactggtccg ccggatggtc gccgagggcc
     8221 acgagctcgg caaccactcc tggagtcatc cggagttctg gaacctctcg aagagcggca
     8281 tccgcaagca gttggtcaag accaacaagc tgctcaccaa gctcggccag cgtccgaagt
     8341 tggtgcgtcc gccgtacggg gagtgggatc gcgatgtggc caaggtgacc aggtcgctcg
     8401 gcatgcctgc cgtgatctgg gacgtggatc ccgaggattg gaaggtgcgc aaggccaaga
     8461 cggtcgcgaa gcgcgtgctg aagcaggtgg agcgaggttc gatcatcctc acccacgaca
     8521 gcctgaagtc gacccggaag gcgatcccgg cgatcgtcag aggcctccag gccaagggct
     8581 acacccttgt caccgtctcc cagttgatgg ccggcaagtt gcacccgggc aagcgctgct
     8641 tccacgcctg acccagatcc ggccacttca gccccgcgcg gcgcggtacc gggcgagcgc
     8701 ctccaacctc tccctgccat gatccagaat cggacgcgga tagccgtggc ggtaaccgtc
     8761 cgcggccttc gacggctcgt gcgccgttgc ccccctcagg tggcgcagtt ctggcaccca
     8821 gcgccggacg tagtcgccgt tcgggtcgaa gcgaaggccc tggctggtgg gattgaagac
     8881 ccgaaagtag ggcgccgcgt cggttccggt gccggccgtc cattgccaac cgtgcgcatt
     8941 ggaggccagg tcgccgtcga tcaggtgatc caggaagtgc gtggccccca gcggccacca
     9001 gtgatgcagg tccttggtga ggaaactcgc ggtgatcatc cggacgcggt tgtgcatcca
     9061 accctcggcc agcaactggc gcatcccggc atcaacgatc ggaaagccgg tccgccccag
     9121 ccgccaagcc tcgaagccgg ccgacggctc gtcatagtcc agcttgatgc tcttcaggtc
     9181 cgcagccagt gattcggggt gccggctgag cacgtcggcg tagaactccc gccaggcgag
     9241 ttcggtggcg tagcggtcgg cgtccggccc gggacgctcc gccaagtcgg cgagcagggt
     9301 tcgtgggtgt actacaccga acttcaagtc gggtgaaagg cgggaggtgg tgtccaggtc
     9361 cggacgatcc cgatcgccgg agtagccgga caggtctgcg tcaaggaacc gccgccagcg
     9421 tccgagggcg gccgcctcgc cggcgtccgg caacgcaatc ggacacgctg ccagcgcggc
     9481 ctcgatccgt ccccatgccg ccgggttacc ggcggcccgc atgggcgtga aggcgtccgg
     9541 cagcagcgcc ggcccgcgcc agccgtgcgc ctgccaggct cggtggaagg cggtgaagac
     9601 ctgaaagcca ctgccgctca gtgtccggac ccggccggga gtaaccgcgt acggcgagcc
     9661 ggtttccacc cactcgaccc cggccgcggc cagcgcccgc tgcacccgct ggtcgcgcgc
     9721 caatccgccg ggttcggtct cggtggagac gtgcaccacg acagcgccga cctcggcggc
     9781 caactcgggc accacccgaa ccggatcgcc ccagcgcagg cagagccgag agtcgagggc
     9841 ctcgcggaga gcaaccaggt tcgccgccag ccagccctgg cgaaccgcgc cggcattgtc
     9901 gaagaaggcc gggtcgagga cgaagagcgg caccacgggc ccggcggcgg ccgctgcggc
     9961 aagggccggg tggtcgccga gccgcaggtc gcgccgaaac cagagcagcc tgggttcagc
    10021 catgccggct cgcggcgagg gttctggcgg ccgggccgat cagatgcccg agcgtccgcg
    10081 ccggtgccgg tacgaggtgc tggaagcgtc cccccaccca caccgagacc tccggcaagg
    10141 actccagttg ggcggccact cgggtcaccc gctcggagtc gcgtcgctgt ggcaccgagg
    10201 tcaccaccgc gaaagcccgg gttcgcaggg ctgcgtcccg ccaggccggc agcggcactt
    10261 tggcgccgag gtagaccgtg gccaggccgg cccgacggca ggcgacggcg aaggccagca
    10321 ggccgagttg gtggtcgacg ccggggggtg cgccgatcag gaccgggggc ccgccctgcc
    10381 cgcggcggac ggagtcgtag gcagcggcaa ggcggcgcat cagggcgtcg gcgacgagat
    10441 gctcaccggc gacggtcacc ttgccgtccg cccaggcctg gccgagccgg gcgagcgccg
    10501 gcagcatcca gtcgtccacc acggtctcga aggcggccgc gccgaagctc tcggtcacga
    10561 tccggtggat cgaggccgcg tccatcgccg ccgcggcttc gatcaactcg gccgaggagt
    10621 cggtcacgtc gcccagcggc ggtggccggc gaagcaactc agcggcagcg gacttcggcg
    10681 accagccggc ctggaccagc tcctgcatgg cccgcaccct ggccagtgtc tggcggtcgt
    10741 agagccggta gccggacgcc gtccgctgcg gggcgagcag ttcgtatcgg cgctcccagg
    10801 cccgcagggt gtgctccgac aggccggtca cctccgcggc ggccttgatc gtcagcagcg
    10861 tggtggtttc gggtatgacc gtgtccggca tgtgtctaag gttacccagc cttgtcttag
    10921 cccggatcca ccgatggggt gggggttgtg ggcgtggcgt gaagcgcgaa tgc
//
LOCUS       DAOPRQ010000075        10914 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_5055_length_10914_cov_10.763054, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000075 DAOPRQ010000000
VERSION     DAOPRQ010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 10914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10914
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5055_length_10914_cov_10.763054"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="PKJ61_09685"
     CDS             <1..542
                     /locus_tag="PKJ61_09685"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytidylate kinase family protein"
                     /protein_id="HOC14135.1"
                     /translation="RQYGLHQLNDIYDHKPKVWELFNDNSVLTIQMMNQTIAAFAARG
                     NVVILGRGGFKVLEGMGDVLNVFVKAPQDIRVARIAKRENLSEDAATDRIKADDELRA
                     RFTRLFYSANWADESLFDLVVDTGVDSDEAALARIVAAVNALQVPGEVDRAAANIDVD
                     PVLNRTIDGVLGRRTAKLG"
     gene            complement(539..1984)
                     /locus_tag="PKJ61_09690"
     CDS             complement(539..1984)
                     /locus_tag="PKJ61_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOC14136.1"
                     /translation="MSYSAVQDPRQIRFGALAAVVGFLVYVEFTSGILQGFYTPLLTD
                     IARHLNVPDADVNWLEGGQMMAAAIIIPAFAKLGDMIGHRRMLLISTALTAVASAGLP
                     LTNNFWMFLLAWSLQAFYIVWLPLETALIYARARSTGHPAALTRRAAGWLVAALEVGV
                     IAGALSAGQLVDVLPLAVVLWIPAVAVAACFFVILLGVKESPELHGGEFDTTGMVLVA
                     IALLGLTGGLFLLRVNGPGSPVAWLAILVGLALFVPFVRWELRQKDPLVDVRMFVNPS
                     LWPVFLTAGLFGVSVLGAQAPLSTFARTDPSVYGYGLGASSGLTSVLIGAYVLALTAG
                     ALLLPLVTRWLSPRWALIGASLLVGLGYLLFLPFHTSFAETLTNMIIAGIGSGALVAA
                     LPAAAAAAAPHGQTGVATGLTNSVKTVGGSVASAVFGVALATHAVGALQGTAGSFSGY
                     VTVWVICGATALVAALALLFVPRTAFSDVVG"
     gene            complement(1984..3291)
                     /locus_tag="PKJ61_09695"
     CDS             complement(1984..3291)
                     /locus_tag="PKJ61_09695"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOC14137.1"
                     /translation="MIRVPTVSAERETQGLAPFEEFVDLLAELYPQLHAGLQLERFDG
                     FGLVYTWASSGSALDPVVLMAHYDVVPVTGQEAEWSVPPFEGRVADGKVWGRGTLDDK
                     GALCVLLEAVENLVAEGWTPPRPVLLCLGPDEEVMGATARLIAATYRDRGVRPWLVLD
                     EGGAITEVPFPGVDGHFAMVGLAEKGVLSVELNASGQGGHASATTGITAVGRIARVVA
                     KLNRNPFDVRMPRTVIDLLGKLAAHAQPKYARLWRLAARNPGPAARALLAGGGADGAA
                     LVRTSLAPTMLSGGSSPNVLPSAASATLNIRVNVGETTTGVIARLRRVINDPLVAVRV
                     LEGDEPTPESPSDNAQFALIAQAVEASYPGTPTLGYLTMAATDARHWHRFTSAVYRFA
                     PLRMSAAQRAAIHGIDESVDIDSLVRGEQFYRALLLNLPGGES"
     gene            3372..4091
                     /locus_tag="PKJ61_09700"
     CDS             3372..4091
                     /locus_tag="PKJ61_09700"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015071606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HOC14138.1"
                     /translation="MTEELARVLAASKRIVFFGGAGVSTESGIPDFRSGDGLYQRGGA
                     RYAPEEILSHSFFVRHPEEFFDYYRTNLLHPNARPNPAHLALARLEAAGRLTAVITQN
                     IDGLHQAAGSKRVLELHGSVHRNRCQRCGRGFGLDAILRSSGLPRCDCGGIIKPEVVL
                     YEEALDPDVLDAALSHLAAADTLVVGGTSLVVYPAAGLVREFRGRNLVLINREPTGYD
                     DLATLVVREPIGAAFGAAVPG"
     gene            4146..6020
                     /gene="ligD"
                     /locus_tag="PKJ61_09705"
     CDS             4146..6020
                     /gene="ligD"
                     /locus_tag="PKJ61_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007076669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HOC14139.1"
                     /translation="MAAAGTQHVTVAGRTLALTNLDKVLYPATGTTKGEVIAYYAAIG
                     DVLLPHLTMRPITRKRWPDGVGDGGPDVNVFFAKNLPRGTPDWVKRFPIEHSDGTNLY
                     PIADDVATLVWLAQLASLELHVPQWRFADKGTPRNPDRLVLDLDPGPGVTLSQCAEVA
                     GWVRDALRAVGLEPYPVTSGSKGIHLYTHLDGTLTPTEASALAKRLAEALQAKEPARV
                     TAVMRRADRDGKVFLDWSQNNGNKTTITPYSLRGRAHPTVAAPRTWDELSRPGLVQLD
                     FHEVLDRVAELGDLLAPLAGEPESRPARPAGVSPARPMLAKLATTAEVGSGDAWAFEM
                     KWDGVRVLIEIEGEQIRLVSRSGRDETLRYPDLMADLRAIGAGEAILDGEIVVVDAGG
                     APRFELLQPRINLTKPADIAAAAQRAPVQLMLFDVLSLNGVDLTELAYEERRKRLEEL
                     PAQGRVQVPPGFEGDLAAALDTSLALGLEGVVAKRRGSAYAAGSRSRNWLKIKHRRTQ
                     SVVIGGWRPGSGNREGTIGSLLVGVPSVQGLRYSGRVGSGFSDAGLAEAQRLLRELSR
                     AEPSLVDVPKEDAKDAAWVEPLLVGEVAYTERTSMGRLRHPVWMGWRTDLTPADVHPE
                     "
     gene            6030..6434
                     /locus_tag="PKJ61_09710"
     CDS             6030..6434
                     /locus_tag="PKJ61_09710"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOC14140.1"
                     /translation="MAAGGAMDVVAAVFTDGDLVLAGRRKPYLAAGGRWEFPGGKTVA
                     GEAPQAALEREIREELSVEIEVGDLADLSATMVNGTLIRLACYFVRPLAEVPATSTDH
                     DVVSWFRRERLMWLDWAEPDLPCVRRLAFAAS"
     gene            6466..6960
                     /locus_tag="PKJ61_09715"
     CDS             6466..6960
                     /locus_tag="PKJ61_09715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14141.1"
                     /translation="MALFARTRLDPELKEAAGRRRALASGTSPEGQVAGLVDALLFRQ
                     DGAWQEQPWHRIERGSWDAETHSLNWLDVEGRSWRLALSEPGQLPDFFNERVTASIAC
                     VRTADLGRGRTALITARRDLGDGSRPLVWRVSPGKGVRADELAQDPLVAIALEQLRAE
                     FDAR"
     gene            6996..7068
                     /locus_tag="PKJ61_09720"
     tRNA            6996..7068
                     /locus_tag="PKJ61_09720"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7029..7031,aa:Asn,seq:gtt)
     gene            7070..8752
                     /locus_tag="PKJ61_09725"
     CDS             7070..8752
                     /locus_tag="PKJ61_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14142.1"
                     /translation="MDTAARPNRRDRALTWLFIAIPVLVTTSMVWLRRWVSDDGFINY
                     RVIQQLMAGRGPIYNLNDRVEVGTSTLWLGLVWLGQAIVPSAETGMVMVVLGTALTIV
                     ALSCLALGAWALNPGRRLLVPVGLAVVAALPPVWDFGTSGLETSLSMAWIAGCFAALA
                     FRLRGGKDRPAWQPWPIAMLIGLGPLVRPDFALIALFFGIALLWQSRRRWLDWLAAAG
                     IALVLPLAYEVFRMGFFASLVPNTALAKANGSLAQGVSYVLDFVGTYWLWVPLAIGAW
                     LLGARILRRPSVGEPDARSIYVIFPLTALVHVAFVVSVGGDFMHGRFLLPATFLFFAP
                     IAMVPFTGVRRVLAPMVVAWALCCGLLLRPALWNGMIADERTYYAAFVADVPGAMVSL
                     ANWSDDIGFKLARAAASDHARGKSYYVTVVQPDERLGRTGPGVVMVLNSLGVAGAASG
                     LDVVVNDPPSLGDAIGARLVLPPDAEFRVGHAYKPEVWALARFASEDRTPPVAGLAEA
                     RQALACEPVVELLDAVSEPLTFERFVKNVTLAPSLTFLRIPVDPAEAVQKLC"
     gene            8833..9984
                     /locus_tag="PKJ61_09730"
     CDS             8833..9984
                     /locus_tag="PKJ61_09730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14143.1"
                     /translation="MADRTDEEIKAAERKAVADAISAEHDAAKKRLEVEAAERNAIKD
                     LVADLSGVDHGSLTVPDSSTVFQSLLGSRALADAAAKVAPIVAGKLKNGAKVLVITDL
                     NLAGRDLSYRSVIDQLKQLTTLTSTYPDAPQENKGIDDVRMIDGVTGVATAAAKLLPG
                     LLSLISARRTITNTSGTADDDVAAIAVAGALASTTKQVVVLDKARLIEADGAAQTAWT
                     DLRTACDGLAEKLKAAPESSATKGWLAGGNELLKKAEAVLSAMVASDGAEPSALAKAS
                     LQEALHDAGGFDAVLVVKGGATLSSQVVDDRPLLMKDKISLVSTATVSYVLIDHKNGG
                     RVLAGGLAVGEAQLHGVLGDELLPTTPQQEPVHDHTDPMEHGLGGAGPS"
     gene            10082..10552
                     /locus_tag="PKJ61_09735"
     CDS             10082..10552
                     /locus_tag="PKJ61_09735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14144.1"
                     /translation="MGLFNNIKDALTTDDAERAEAARMPEPTVEPVPQVEPPVVEAPA
                     PVVVEKPETKPAKPKDDEPKLTVDGKFGPRTIAATQRWVGAKVDGVWGVQSKRALQDK
                     LGVKEDGEVGPITIKALQAKIGARVDSMWGPDTVRHLQEYPQQALSSTKGSASV"
     gene            complement(10549..10779)
                     /locus_tag="PKJ61_09740"
     CDS             complement(10549..10779)
                     /locus_tag="PKJ61_09740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14145.1"
                     /translation="MQSDEAWQQPPFGSGLDAIAVDLITELVQQVFADLDPVQLVEAS
                     GRPLNEADKRTVAHLIDHAVVELEVDFGLAHD"
BASE COUNT         1808 a         3563 c         3831 g         1712 t
ORIGIN      
        1 tccggcagta cgggctgcac cagttgaacg acatctacga ccacaagccc aaggtgtggg
       61 agctgttcaa cgacaacagc gtgctgacga tccagatgat gaaccagacc atcgcggcgt
      121 tcgccgcgcg cggcaacgtg gtgatccttg gccgcggcgg tttcaaggtg ctcgaaggca
      181 tgggcgacgt gttgaacgtg ttcgtcaagg cgccgcagga catccgggtg gcccggatcg
      241 ccaagcggga aaacttgtcc gaggatgctg ccaccgatcg gatcaaggcc gacgacgagc
      301 tgcgcgcccg cttcacccgg ctgttctatt cagccaactg ggccgacgag tccctgttcg
      361 atctggtcgt ggacaccggg gtcgactccg acgaggccgc gctggcccgc atcgtggcag
      421 cggtgaacgc actgcaggtt ccgggcgagg tcgatcgggc cgcggccaac atcgacgtcg
      481 atccggtcct caaccgcacc atcgacggcg tgctcggacg ccgcacagcc aagctgggct
      541 aaccgaccac gtcgctgaac gccgtccgcg gcacgaacag aagggcgagc gcggccacca
      601 gggcggtggc cccgcagatc acccagacgg tcacatagcc gctgaaggag ccggcggtgc
      661 cctgcaaagc gccgaccgcg tgggtcgcaa gggcgacgcc gaacaccgcg gacgccaccg
      721 agccgcccac cgtcttcacc gaattggtga gtccggtggc gacgccggtc tggccgtgcg
      781 gagcggccgc agccgctgcg gccggcagcg ccgcgaccag tgcgcccgac ccgatgccgg
      841 cgatgatcat gttggtcagc gtctcggcga agctggtgtg gaagggcagg aagagcaggt
      901 agcccagacc gaccagcagg ctcgctccga tcagggccca gcgcgggctg agccaacggg
      961 tcaccaacgg cagcaacagt gcaccggccg tgagggcgag cacgtaggcc ccgatcagaa
     1021 ccgacgtgag tccgctgctg gcgcccagcc cgtagccgta gacggacggg tcggtgcggg
     1081 cgaacgtgga cagcggcgct tgggcaccga gcacgctcac gccgaacaac ccggcggtga
     1141 ggaacaccgg ccacagcgat gggttcacga acatccgcac gtccaccaag gggtccttct
     1201 ggcgtagttc ccagcgaacg aacgggacga acaacgccaa tcccaccagg atcgcgagcc
     1261 aggcgaccgg gctgcccggt ccgttcacgc gcagtaggaa gagcccgccg gtaaggccga
     1321 gcagcgcgat ggcgaccagc accatgccgg tggtgtcgaa ctcgccgccg tgcagctccg
     1381 gggactcctt caccccgaga aggatgacga agaagcacgc ggccacggca accgccggga
     1441 tccacaacac gaccgcgagt ggcagcacat ccaccaactg tccggccgaa agcgcgccgg
     1501 cgatcacccc cacttccagt gcggcgacca gccagccagc ggcccgtcgg gtgagggcgg
     1561 ctgggtgacc ggtgctgcgg gcgcgggcgt agatcagcgc ggtctccagc ggcaaccaga
     1621 cgatgtagaa ggcctgcagc gaccaggcga gcaggaacat ccagaagttg ttggtcagcg
     1681 gcaggccggc gctcgcgacc gcggtgagtg cggtcgagat cagcagcatc ctgcggtggc
     1741 cgatcatgtc gccgagcttg gcgaaggccg gaatgatgat cgcagcagcc atcatctggc
     1801 cgccttcgag ccagttcacg tccgcgtccg gcacgttcag atgcctggcg atgtcggtga
     1861 gcagcggggt gtagaagccc tgcaggatgc cgctggtgaa ctcgacgtac accaggaacc
     1921 cgacgacggc cgcgagcgcg ccgaaccgga tctggcgggg atcctgaacg gcgctatagc
     1981 tcatcacgac tcccctccgg gcaggttgag cagcagcgcg cgatagaact gctcgccgcg
     2041 gaccagactg tcgatgtcga cggattcgtc aatgccgtgg atcgcggccc gctgcgccgc
     2101 cgacatccgc agcggcgcga agcgatagac cgccgaagtg aagcggtgcc agtgccgggc
     2161 atcggtagcg gccatggtga ggtaaccgag cgtcggcgtg ccgggatagc tggcctcgac
     2221 ggcttgcgcg atcagcgcga actgggcgtt gtcggacggc gactcgggag tcggctcgtc
     2281 tccttccagc acgcggacgg cgaccaacgg atcgttgatc acccggcgca ggcgggcgat
     2341 cacgccggtg gtggtctcac cgacgttgac ccggatgtta agcgtcgccg aagccgcgga
     2401 cggcagcaca ttggggctgc tgccaccgct cagcatcgtc ggcgccaggc tcgttcggac
     2461 gagcgcagcc ccgtccgcac caccaccggc cagcagggct cgggcggccg gccccgggtt
     2521 cctggcggcg agccgccaga gccgggcgta cttcggctgg gcgtgcgcgg caagcttgcc
     2581 gagcaggtcg atcacggttc ggggcatccg gacatcgaac gggttgcggt tgagcttggc
     2641 gaccactcgg gcgatgcgtc cgaccgctgt gatcccggtg gtggcggagg cgtgtccacc
     2701 ctgccccgaa gcgttcagtt caacactaag cacgcccttc tcggccagtc cgaccatcgc
     2761 gaaatggccg tccacgcctg ggaacggcac ctcggtgatc gcgccgcctt cgtcaagcac
     2821 cagccatggc cgcacgccgc ggtcgcggta ggtggcggcg atcagcctgg cagtcgcacc
     2881 catcacttcc tcgtcggggc caaggcacaa cagcaccgga cgcggcggcg tccaaccttc
     2941 ggccaccagg ttctcgaccg cttccagcag cacgcacaag gcacccttgt cgtcgagggt
     3001 gccgcgtccc cagacctttc cgtcggcgac gcggccctcg aaaggcggga cggaccactc
     3061 ggcctcctgg ccggtgaccg gtactacgtc gtagtgcgcc atcagcacga ccggatccag
     3121 ggctgagcct gacgatgccc atgtgtaaac cagcccgaac ccatcgaagc gctcgagctg
     3181 caaccccgca tgcaactggg ggtagagctc agcgagcagg tcgacgaact cctcgaaggg
     3241 cgccaagccc tgagtctcgc gctcagccga gaccgttggc acccgaatca tcgccgcgag
     3301 tcgatccgct atcccctgtg acatgcgcgc agactatcgc cgcccactga cgtcgtcgct
     3361 agcctcgatc cgtgaccgaa gagttggcgc gagtcctggc cgcgagcaag cggatcgtgt
     3421 tcttcggcgg cgccggtgtc tcgaccgaga gcggcatccc cgacttccgc tccggcgacg
     3481 gcctgtacca gcgcggtggc gctcgctacg ccccggaaga gatcctcagc cacagcttct
     3541 tcgtccgcca cccggaggag ttcttcgatt attaccggac caacctgctg cacccgaacg
     3601 cccgaccgaa ccctgcgcac ctggccctgg ccaggcttga ggcggcggga cggctgaccg
     3661 cggtgatcac ccagaacatc gacggcttgc accaggccgc cggctcgaag cgggtgctcg
     3721 agctgcacgg cagcgtgcat cggaaccgct gccagcgctg cggtcgcggc ttcggcctgg
     3781 acgcgatcct gcgctcatcc ggtctgccgc gctgcgattg cggcgggatc atcaagcccg
     3841 aagtggtgct ctacgaggag gcgctcgacc cggacgtcct ggatgctgcg ctcagccacc
     3901 tggcggctgc cgacaccctc gtcgtcggcg gcacctccct ggtggtgtac ccggcagccg
     3961 gactggtgcg cgagttccgc ggccggaacc tggtgctgat caatcgggag ccgaccgggt
     4021 atgacgatct cgccacgctg gtggtccggg agccgatcgg cgcagccttc ggcgctgcgg
     4081 ttccgggctg agggctgcca gaactgccac tcgacccgcc ggtgggggtc cggcaggatg
     4141 tggccatggc ggccgcgggt actcagcacg tgaccgtggc cggtcgcacg ctggcgctga
     4201 ccaatctgga caaggtgctc tatccagcca ccggcaccac caagggtgaa gtgatcgcct
     4261 actacgccgc gatcggcgac gtgctgctgc cgcacctgac gatgcggccg atcacccgga
     4321 agcgctggcc ggacggggtg ggcgacggcg gtccggacgt gaacgtgttc ttcgccaaga
     4381 acctgccccg cggtaccccc gactgggtga aacggttccc gatcgagcat tcggacggga
     4441 ccaacctcta tccgatcgcc gacgatgtag ccaccctcgt ctggctcgcg cagttggcat
     4501 cgctggaact gcacgtgccg cagtggcgct tcgccgacaa aggcaccccg cggaatccgg
     4561 accgactggt gctcgacctc gatccggggc ccggcgtgac gctcagccag tgcgcggagg
     4621 tggccggctg ggtgcgggac gccctgcgtg cggtcgggct ggagccgtac ccggtcactt
     4681 cgggcagcaa gggcatccac ctgtacacgc acttggacgg gacgctgacg ccgaccgagg
     4741 cgtccgcgct ggccaagcgc ctggccgagg ccctccaggc caaggaaccc gcccgggtca
     4801 ccgcggtgat gcgtcgcgcc gaccgggacg gcaaggtctt cctcgactgg agccagaaca
     4861 acggcaacaa gaccacgatc actccgtatt cgttgcgcgg acgcgcccat cccacggtgg
     4921 ccgcccccag gacctgggac gagctgtccc gtcccgggtt ggtacaactg gacttccacg
     4981 aggtgctcga tcgcgtggcc gagctgggcg atctgctcgc accgctggcc ggcgaaccgg
     5041 agtctcgacc ggctcgacca gccggggtca gcccggcccg accgatgctc gccaagctcg
     5101 ccaccacggc cgaagtcggt tccggcgacg cctgggcgtt cgagatgaag tgggacggcg
     5161 tccgcgtcct gatcgagatc gaaggcgaac agatccggtt ggtgagccga agcggacgcg
     5221 acgagacgct gcgctatccc gatctgatgg ccgacctgcg cgccatcggc gctggtgagg
     5281 ccatccttga cggcgaaatc gtcgtcgtcg acgccggtgg agcgccacga ttcgaactgc
     5341 tccagccgcg gatcaacctc accaaaccgg ccgacatcgc ggcggcggcc cagcgggcac
     5401 ctgtgcagtt gatgctgttc gacgtactga gcttgaacgg tgtggacttg accgaacttg
     5461 cctacgagga gcgccgcaag cggcttgaag agctgccggc ccagggtcgc gttcaggtac
     5521 caccagggtt cgagggcgat ctggccgcgg ccctggacac cagcctggcg ctcgggctgg
     5581 agggcgtggt ggccaagcgc cgcggctcgg cgtacgccgc aggttcgcgc tcgcgcaact
     5641 ggttgaagat caagcaccgg cgaacccagt cggtggtgat cggcggttgg cgtcccgggt
     5701 ccggcaaccg tgaaggcacc atcggctcgc tgctggtcgg cgtcccgagc gtgcagggcc
     5761 tgcggtactc gggacgcgtg ggctccggtt tcagcgacgc cggcctggcc gaggcgcaac
     5821 ggttgctgcg cgaactgtcg cgagcagagc ccagcctggt cgatgtgccc aaagaagacg
     5881 ccaaggacgc cgcctgggtg gaaccgctgc tggtgggcga agtggcctac accgaacgca
     5941 cctcaatggg acggctgcgg cacccggtct ggatgggttg gcgaaccgat ctgaccccgg
     6001 ctgacgtaca tccggagtga gggtggagaa tggcagcagg gggcgcgatg gacgtggtgg
     6061 cggcagtttt caccgacggt gatctggttc tggccggtcg gcggaagcct tacctggccg
     6121 ccggcgggcg ctgggagttc cccggtggga agaccgtcgc gggcgaagcc ccccaggccg
     6181 cacttgagcg ggagatcagg gaggaactca gcgtcgagat cgaggtgggc gacctcgccg
     6241 atctgagcgc cacgatggtg aacggcactc tcatccggct ggcgtgctac ttcgtccgcc
     6301 ccttggctga ggtgcccgcg accagcaccg atcatgacgt ggtgtcctgg ttccgacgcg
     6361 agcggttgat gtggctggac tgggcggagc ctgatctgcc gtgcgtgcgg cggctggcgt
     6421 tcgctgcgtc ctgagcgact cctcggatca agcagaatgg gcggcatggc actcttcgcg
     6481 cgcacccgat tggaccccga gctgaaggaa gccgccggac gacgtcgcgc gctggcgtcc
     6541 gggacaagcc cagaaggcca ggtcgccggg ttggtggacg cgttgctgtt ccggcaggac
     6601 ggtgcctggc aggagcagcc gtggcaccgg atcgagcgcg gcagctggga cgccgagacc
     6661 cactcgctca actggctgga cgtggagggg cgctcctggc gcctggcgtt gtccgagccg
     6721 gggcagctgc cggacttctt caacgagcgc gtcaccgcga gcatcgcctg cgtccgtacg
     6781 gcggatctgg gacggggccg gacggcgctg atcaccgccc gccgcgacct gggtgacggc
     6841 tcgcgtccgc tggtctggcg agtgagcccc ggcaagggag ttcgggccga tgaactggca
     6901 caagacccac tcgtggcgat cgcgctggag cagttgcgcg ccgagttcga cgcccggtga
     6961 agttctggta atgtaacggc tcgcgcccag cgcgatcccc tgtagctcaa ttggcagagc
     7021 gtcggactgt taatccgcag gttcctggtt cgagtccagg cgggggagct tggatacagc
     7081 ggcccgtccc aatcggcgtg atcgtgccct gacttggctc ttcatcgcaa tccccgtgct
     7141 ggtgaccacc tcgatggtct ggcttcgacg ctgggtctcc gacgacggct tcatcaacta
     7201 ccgcgtgatc cagcaattga tggccggacg cgggccgatc tacaacctga acgatcgcgt
     7261 cgaggtgggc acgagcacgc tctggctcgg gttggtgtgg ctcggccagg cgatcgtgcc
     7321 gtccgccgag accggaatgg tgatggtggt gctcggcacc gcactcacca tcgttgcgct
     7381 gagctgtctg gcgctgggcg cctgggcact caatcccggt cgccgcctat tggtgccggt
     7441 cgggctcgcg gtggtggccg cgctcccccc ggtctgggac ttcggcacgt ccggcctgga
     7501 gacttcactc tcgatggcct ggatcgccgg gtgcttcgcc gcactggcgt tcaggttgcg
     7561 cggtggcaag gatcggccgg cctggcagcc ctggccgatc gcgatgctga tcgggctcgg
     7621 cccgttggtc cgcccggact ttgcgctgat cgcactattt ttcggcatcg cactgctgtg
     7681 gcagagccgg cggcgctggc tcgactggct ggccgcagcc ggaatcgccc tggtgctgcc
     7741 gctggcctac gaggtgttcc ggatgggttt cttcgcctcg ctggtgccga acaccgcgtt
     7801 ggccaaggca aacggctcgc tggcgcaggg cgtcagctac gtgctcgact tcgtcggcac
     7861 ctactggctg tgggtgccgc tggcgatcgg cgcctggctg ttgggcgcgc ggatactgcg
     7921 gcgtccgtcc gttggcgaac cggacgctcg atcgatctac gtcatcttcc cgttgaccgc
     7981 gctggtgcac gttgcgttcg tggtgagcgt gggcggcgac ttcatgcacg gacgcttcct
     8041 gctgccggcc acgttcctgt tcttcgcacc gatcgcaatg gtgcccttca ccggcgtccg
     8101 gcgggtgctg gcccccatgg tcgtcgcgtg ggcgctctgc tgcgggctgc tgctgcgtcc
     8161 ggcgttgtgg aacggcatga tcgcggacga gcggacgtac tatgcggcct tcgtcgcgga
     8221 cgtgccgggt gcgatggtgt cgctggcgaa ctggagtgat gacatcggct tcaagctggc
     8281 gcgggctgcg gcgtccgatc acgcgcgggg caagagctac tacgtgaccg tggttcagcc
     8341 ggacgaacgg ctgggacgca ccgggccggg cgtggtgatg gtgttgaaca gcctcggggt
     8401 ggccggggcc gcgtccggtc tggacgtggt ggtgaacgac ccgccatcgc tcggggacgc
     8461 gatcggcgcc cgcctggtgc tgccgccgga cgccgagttc cgggttggtc acgcgtacaa
     8521 gccagaggtg tgggcgctgg caaggttcgc gtccgaagat cgcactcccc cggtggcagg
     8581 cctggccgag gcgcgtcagg cgctggcgtg cgagccggtg gtcgagttgc tggacgcggt
     8641 ttcggagccg ctcacgttcg aacgcttcgt gaagaacgtc acgctcgctc cgtcgctcac
     8701 cttcctgcgg attccggtcg acccggctga ggccgtccag aagctctgct gaaatcagcg
     8761 tcgaggctct tacgccgcgc caccgaccct tctaggctgc atctcaactg cggaccagga
     8821 atcgggggaa tcatggccga tcgaactgac gaagagatca aagcagccga gcgcaaggct
     8881 gtggcggacg cgatcagcgc cgagcacgat gctgcgaaga agcggctgga agttgaggcc
     8941 gccgagcgga acgccatcaa ggacctggtg gccgacttga gcggggtgga tcacggatcg
     9001 ctgaccgtgc ccgattcatc aacggtgttc cagtcgctgc tcggctcgcg ggctttggcg
     9061 gacgctgccg cgaaggttgc gccgatcgtc gccggcaagc tgaagaacgg tgccaaggta
     9121 ctagtgatca ccgacctcaa cctcgccggg cgcgacctga gctatcgctc ggtgattgac
     9181 caactcaagc agttgaccac actgaccagc acctatccgg atgcccctca ggagaacaag
     9241 gggatcgatg atgttcgaat gatcgacggc gtcacggggg tggcgaccgc tgcggccaag
     9301 cttctgcctg ggctgctgtc actgatctcg gcgcggcgca cgatcaccaa cacgagcggc
     9361 accgcggacg acgacgtggc cgcgatcgct gtggccggag ccctggccag cacgactaag
     9421 caggttgtag tgctggacaa ggcgcgtctg atcgaggccg acggcgcggc gcagaccgca
     9481 tggaccgacc ttcggacggc ctgcgacggc ctcgcggaga agctcaaggc cgcccccgag
     9541 agctccgcga ccaagggttg gctcgcgggt ggtaacgagt tgttgaagaa agcggaggcc
     9601 gtgctgtcgg cgatggtggc cagcgatggt gccgagccga gcgcactggc gaaagcgtcg
     9661 ctgcaagagg cgctccacga tgctgggggc tttgacgcgg ttctggtggt caagggtggc
     9721 gcgacgttgt cctcccaggt agtcgacgac cgtccgctgc tgatgaagga caagatcagc
     9781 ctggtcagta cggcgacggt gtcgtacgtc ctgatcgacc acaagaacgg tggccgggtg
     9841 ctcgctggtg gtctggccgt cggtgaagcg cagctgcacg gtgtgctcgg cgacgaactg
     9901 ctgccgacta cgccccagca ggaaccggtg cacgaccaca ccgatccgat ggagcatggt
     9961 ctgggcgggg cgggcccctc ctaaaccgta gctggaccgc acccttgcct cttgactccg
    10021 gcttgacagc tggtgaaatc tgacgggctt gccgccacac ccgctggtgg gaggactgga
    10081 aatgggactg ttcaacaaca tcaaggacgc actcaccacc gatgacgcgg agcgcgccga
    10141 ggcggcccgc atgccggagc cgacagtcga gcccgtgccg caggttgagc ctccggtcgt
    10201 ggaggcgccc gctccggtcg ttgtggagaa gcctgagacg aagccggcca agcccaagga
    10261 cgacgagccg aagctgacgg tggatggcaa gttcggcccg cgaacgatcg ccgcgacgca
    10321 gcggtgggtc ggcgcgaaag tcgatggggt ctggggcgtg cagtcaaagc gagcgctcca
    10381 ggacaagctg ggtgtgaagg aggacggcga ggtcggccca atcacgatca aggcgctcca
    10441 ggcgaagatc ggcgccaggg tcgacagcat gtggggcccg gacaccgtcc gccacctgca
    10501 ggagtaccct caacaagcac tgagctcgac gaaggggtcg gcttcggtct agtcgtgggc
    10561 caggccgaag tcgacctcaa gctccaccac ggcgtggtcg atgaggtgtg caacggtgcg
    10621 cttgtcggct tcgttcagtg gccgtccgga cgcttcgacg agttggacgg gatcgaggtc
    10681 agcgaatacc tgctggacga gttcggtgat caggtcgacc gcaatcgcgt ccagcccgct
    10741 gccgaacggc ggctgctgcc aagcctcgtc tgattgcatg tgattcctgc cggttagctt
    10801 gtgccgaagg ctacccgcgt ggacaccgca gccaggcccg gctacgttga ttcgcgacac
    10861 caccggggcg gtagctcagc cggtcagagc aggggactca taatccctgg gtcg
//
LOCUS       DAOPRQ010000076        10878 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_5085_length_10878_cov_13.520743, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000076 DAOPRQ010000000
VERSION     DAOPRQ010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 10878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10878
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5085_length_10878_cov_13.520743"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(79..408)
                     /locus_tag="PKJ61_09745"
     CDS             complement(79..408)
                     /locus_tag="PKJ61_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family metal-binding protein"
                     /protein_id="HOC14146.1"
                     /translation="MSRSEEDGWPQTALELMRSRFEAFRDGDAAWLLASWHPSTRPAN
                     LDLTGNPTWRGLQIVDTLDGGPDDDTGIVEFRATCLAPRGGVEVQQERSRFVREGGRW
                     YYLAPLR"
     gene            complement(469..1326)
                     /locus_tag="PKJ61_09750"
     CDS             complement(469..1326)
                     /locus_tag="PKJ61_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016455211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HOC14147.1"
                     /translation="MTALGALRFPTQLYEAYVFDLDGTIYLGEELLPGAKRTIEELRR
                     REIPIRFLSNNATSDPAQVAAKLAGLGLPTDVAEIANTVVTTTQWLKAHHPEAVVFPI
                     SEEPLKRALAAAGIRMSEDPAEIDIVIASYDRTFDYRKPQIAFDAIWFHRRAFLIQTN
                     PDAFCPYPGGRGEPDCAAITAAIEASTDTTCRQSLGKPDPVMLQTVMATLPDADIARS
                     VMVGDRLQTDSRMALAAGMASAVVLTGEATAEDVAALPEQLKPTLVLDRIDRLIPMYA
                     WIELGWAEA"
     gene            complement(1413..2345)
                     /gene="ligD"
                     /locus_tag="PKJ61_09755"
     CDS             complement(1413..2345)
                     /gene="ligD"
                     /locus_tag="PKJ61_09755"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019045792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HOC14148.1"
                     /translation="MAKAEPVEIEVGPRTVRLTSPDRVYFPAHGWTKLDLANYYLAVG
                     DGIVRALRERPCMLHRFPSGLSGGKVHQKRVPAGAPPWLETVQIYFPRYGRTADELCV
                     TELGAVIWAVQMSTVEFHPWNSRRADVERPDEWRIDLDPMPAAGFERVRRVAAVAKTV
                     LDDLGMIGFPKTSGGNGLHIYVRIVPEWSFGEVRRAALAFAREVERRAPDDVTTAWWR
                     QERDPRHVFVDYNQNARDHTIASAWSVRGVPDGTVSTPFHWSELDAIAPRELTLATVP
                     ARFAKLGDPHAAIDDVAHRLDTLLEWADRDGLPE"
     gene            complement(2429..2611)
                     /locus_tag="PKJ61_09760"
     CDS             complement(2429..2611)
                     /locus_tag="PKJ61_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14149.1"
                     /translation="MSTMPWGTMNDWLRVEAEQRNAWTQLRVQDLRAAQVERRSARTV
                     RRPALRPTPATCVEAA"
     gene            2719..3297
                     /locus_tag="PKJ61_09765"
     CDS             2719..3297
                     /locus_tag="PKJ61_09765"
                     /inference="COORDINATES: protein motif:HMM:NF024701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOC14150.1"
                     /translation="MGYHHGDLRTAMIDAGTTLVAEGGLPALSVAEAARRTGVSAAAP
                     YRHFPTRQHFLIAVAAKAAQDLDAAMRTAADAAGPDPVEAIAAAAAAYVRFAATRRAG
                     FDLVYAPELRGVHDEELREAGRSVSDIVLLRAIEATGGDSRAAVDIVPQFMIVAQGAA
                     SMHLIGILSDVESAARLAAKTIRSLLRSAPGE"
     gene            3578..3727
                     /locus_tag="PKJ61_09770"
     CDS             3578..3727
                     /locus_tag="PKJ61_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14151.1"
                     /translation="MAAAKGAIKGFFHIFTADHLFTHARNRPEETPYHLVTILDVLEP
                     QRPRR"
     gene            3761..3991
                     /locus_tag="PKJ61_09775"
     CDS             3761..3991
                     /locus_tag="PKJ61_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14152.1"
                     /translation="MLTTSQAAALVGLPKEQFRSAMSKERKNGKEFHAPKELWADART
                     PLWDEKKVLAWAKDRKKRKKRKKEEKEEKEAS"
     gene            complement(3995..4963)
                     /locus_tag="PKJ61_09780"
     CDS             complement(3995..4963)
                     /locus_tag="PKJ61_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020575794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HOC14153.1"
                     /translation="MRAAYVKAANPADPLAALVVGELPEPEVPDGWTRVRVRAASLNH
                     HDLWSLRGVGLPPERMPMILGTDAAGTTDEGREVIVHAVISSPDWSGDETLDPKRTLL
                     SELHQGTLADYVVVPNANLIEKPANLSFEDAACLPTSWLTAYRMLTRNGLAPGDTILI
                     QGASGGVASAAIQLGRQLGLRVWATARTEAKRAFALELGADAAFEAGARLPQRVDAVI
                     ETVGEATWSHSLKALKPGGTIVVCGATSGYNPPAELNRVFFLQLRVHGSTMGTREELA
                     QLAELVAATGLKPRIDRALPLAEVASGLAALDAGAVLGKVMVSAGG"
     gene            complement(4981..5328)
                     /locus_tag="PKJ61_09785"
     CDS             complement(4981..5328)
                     /locus_tag="PKJ61_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020391278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5997 family protein"
                     /protein_id="HOC14154.1"
                     /translation="MKPATAASKLGIYLPAAPEEFQRTPITRQELDELRANPPQWLVD
                     LRRNGPFPRQVVAQKLGVSRSGLARGGIEDALTADQIGELIANPPGWLLHERDSYRAV
                     IAEQERVRLAREA"
     gene            5511..6221
                     /locus_tag="PKJ61_09790"
     CDS             5511..6221
                     /locus_tag="PKJ61_09790"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HOC14155.1"
                     /translation="MTGDSAQAAEATLRVGFVPGVTLTKWRRIWGERFPREPLEVSEV
                     AEAEQRRVLVEGAVDLCFIRLPIERTGLHAIPLYEEVPVVVVPKDHPLAAFDDVELAQ
                     LADLLAVDPQAHDPFDLVAYGQGVLRVPHSIARSGSRRDLVYRPIIDAEPTTVALAWR
                     IDNANELIEEFIGVVRGRTVNSSRTAQSRAANKAANKSAGSNQVTQANQDQPVAVQKR
                     AKAKSSTGRPSRRRARRP"
     gene            6362..7441
                     /locus_tag="PKJ61_09795"
     CDS             6362..7441
                     /locus_tag="PKJ61_09795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14156.1"
                     /translation="MFVKSSAGSRKSLKRPIALTIAGALVAAGLTGLLASGAAAEPVL
                     PPLTAAELLGKVAAADVDGLSATFEQRSDLGLPALPAELGDDDLSSVLALLTGTHTVR
                     VWVAGHDQAKAALIDGSTESSITRNGQDVWAWSSAEQEAVHSKLPDAAPSGSSTPTSP
                     TAIISDLLTKLEPSTEVAVASTGYVAGRAVYQLVLSPRDEASLVNQVRVAVDAEEFVP
                     LGLRVIADDGQDAVSVAATSVDFSVPDASVFAFQPPAGVKVTEKKTDAASTTKPGDKA
                     SDRALPKTFGSGWATVAVLDVPKGETSDEQAKILLDSLPKVSGVWGQGRLFSTSLVNA
                     VLTDDGRVAVGSVVPERLYAALLTK"
     gene            7438..8412
                     /locus_tag="PKJ61_09800"
     CDS             7438..8412
                     /locus_tag="PKJ61_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOC14157.1"
                     /translation="MTLAAEAVADAAADRDGAAITSSGLTKRFGKQLVVDDLALEVPS
                     GAVYGFIGPNGSGKTTTIRMLLGLVRPDAGTCALLGGTMPAAGGEVLPKVGALVEGPA
                     FHPYLSGRANLLRLDAADRRSDPATASARVAAAIDRVGLSAAARKRYRAYSLGMRQRL
                     GIAAALLQPRELLVLDEPTNGLDPQGTREVRHLIGDLAADGTTIFVSSHLLAEVEQLC
                     THLGVMRTGRLVAQGPMRELRALTAIRFQVRTPDAELALATVAALGVTDAARVPAGVA
                     GSLGEVAPERIVAALVNAGVAVAEFAQVTPTLEDLFVQLTGEGFDVSG"
     gene            8399..9265
                     /locus_tag="PKJ61_09805"
     CDS             8399..9265
                     /locus_tag="PKJ61_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020577710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOC14158.1"
                     /translation="MSAAEVASAPSRVRPVAAWARLLASELRLIFGRRRNQVGLATLF
                     GLVVLVGIALKVSSLRHPGSTGGDLMSAVAGNGLFLGFAGLSIEMALFLPLAVSVLAG
                     DAIAGEANQGTLRYLLLAPVSRVRLLVMKATSLAIGALTGAFVVAAGGLLTGIVLFGA
                     RPLTTLSGTSIGIADGLWRLALAVLYLAAGLTALAMIGLFISTLTEQPIAATVATAMV
                     GTLLWILDAVPQLDWLHPWLLVDRWPAFIDLLRDPVFTDRLVAGLWVDAAYAAAFFAL
                     AWLRFRRADITS"
     gene            complement(9346..9987)
                     /locus_tag="PKJ61_09810"
     CDS             complement(9346..9987)
                     /locus_tag="PKJ61_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016667379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOC14159.1"
                     /translation="MTTTSTRPETTEAAAKSVSPAAAITVLALTAVLGLAALLGPNLA
                     ASDLGMAMSMGMTHYMELLAANQPWNLLLFMGAPVVLAETLAITELVLLFQREAAPEW
                     VRRTSRIAGLLAGPVMVAILLHLLLNAVVPLTMSGGWRGPADVIAVGFYLSGVIPLAG
                     ITLVELGVVALGRRQAMKWHAIWIAVFLVVAHVAMIFGMLDPGVLGYVMAHQH"
     gene            complement(10423..>10878)
                     /locus_tag="PKJ61_09815"
     CDS             complement(10423..>10878)
                     /locus_tag="PKJ61_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOC14160.1"
                     /translation="AVSALVDRAYVAAREEFARKQVELVAEPTATGLMVDVDPGRIGQ
                     VLANLLSNSLRHTPSGGRVTLAVRPLATTVRIEVADNGEGIAADHLPHVFERFYRADA
                     ARDREHGGSGVGLAISQAIARAHGGGLSVVSAGVGRGATFTLELPRSAS"
BASE COUNT         1685 a         3716 c         3721 g         1756 t
ORIGIN      
        1 gccgacggag gccgcctcga tctgagtcgt ccgtgtcgtc gcgcccattc ccgggagcga
       61 agcttcagcg cctgcgcgct accgcagcgg ggcgaggtag taccagcgac caccctcgcg
      121 gacgaacctc gaccgctcct gctgcacctc gaccccacct cgtggcgcga ggcaggtcgc
      181 ccggaactcg actatgccgg tgtcgtcgtc cggcccaccg tcgagggtgt cgacgatctg
      241 cagtccacgc caggtcgggt tgccggtcag gtccaggttc gccggtctgg tcgacgggtg
      301 ccaggacgcg agcagccaag ccgcgtcccc gtcccggaat gcctcgaagc gcgaccgcat
      361 cagctccaag gcggtctgcg gccagccgtc ctcctcagat ctgctcacgc gcgcaagtct
      421 gccccagcgg gggtcggggc tgagcgactt gctgctcggt gagcgtgcct aagcctcggc
      481 ccagccgagt tctatccagg catacatggg gatcagccgg tcgatccggt cgagtaccag
      541 cgtcggtttg agttgctcag ggagggccgc aacatcctcg gcggttgctt caccggtcag
      601 caccacggcc gaagccatgc cggccgccag cgccatcctg ctgtcggtct gcagccggtc
      661 gccgaccatg accgagcggg cgatgtctgc gtccggcagg gtggccatca ccgtctgcag
      721 catgaccggg tctggcttac ccaggctctg ccggcaggtg gtgtctgtgg acgcctcgat
      781 cgccgcggtg atggccgcgc agtcgggctc gccccgtccg ccggggtagg ggcagaaggc
      841 gtccggattg gtctgaatga gaaacgctcg ccgatggaac cagatcgcgt cgaaggcgat
      901 ttgcggcttg cggtagtcga acgtgcggtc gtaggacgcg atcacgatgt cgatctccgc
      961 cgggtcctcg ctcatccgga tcccggcagc ggcgagcgcc cgcttgagcg gctcctccga
     1021 aatcgggaag acgaccgcct cgggatgatg ggccttcagc cactgcgtgg tggtcaccac
     1081 ggtgttcgcg atctccgcga catcggtggg cagccccagg ccagccagct tggccgcaac
     1141 ctgtgcggga tcgctggtcg cgttgttcga gagaaaccga atcgggatct ctcggcgacg
     1201 caactcctca atcgtccgct tggctcccgg caacagctcc tcgccaaggt agatggtgcc
     1261 gtccaggtcg aagacgtagg cctcgtagag ctgcgtcggg aacctgagcg cgccgagagc
     1321 agtcatagcg gccggtctcc tcactgccct gatccggttg gtcgagcctg tcgagactac
     1381 agcggacgga gcacatcgca gctgcggctg cgctactcgg gcagaccgtc ccggtcggcc
     1441 cactccagga gggtgtccag gcgatgggcg acgtcatcga tcgcggcgtg cggatcgccc
     1501 agcttggcga agcgggcggg cacggtggcc agggtgagtt cgcggggcgc gatcgcgtcc
     1561 agttcggacc agtggaaggg ggtcgacacg gttccgtccg gcacgccgcg gacggaccac
     1621 gcgctcgcga tggtgtgatc gcgtgcgttc tggttgtagt cgacgaagac gtgccgcgga
     1681 tcgcgctcct ggcgccacca cgccgtggtc acgtcgtccg gcgcacgccg ctccacttcg
     1741 cgggcgaacg cgagcgctgc ccggcgcacc tcgccgaagc tccattcggg cacgatccgg
     1801 acgtagatgt gcaggccgtt gccgccggag gtcttcggga agccgatcat gcccaggtcg
     1861 tccagcactg tcttcgctac ggcggccacc cgccgcaccc gctcgaagcc ggccgccggc
     1921 atcgggtcga ggtcgatccg ccactcgtcc ggacgctcca cgtccgcccg ccgcgagttc
     1981 cagggatgga actcgaccgt ggacatctgt actgcccaga tcaccgcgcc gagttcggtc
     2041 acgcagagtt cgtcagcggt gcgtccgtag cgcgggaagt agatctgcac ggtctccagc
     2101 cagggcggcg ccccggcagg tacgcgcttc tggtgcacct tgcccccgga aagcccgctg
     2161 gggaaccggt gcaacatgca tgggcgctcc cgcagcgccc ggacaatgcc gtcgcctacc
     2221 gcgaggtagt aattggccag gtcgagtttg gtccagccgt gcgcagggaa gtacacccga
     2281 tcagggctgg tcagccggac cgtccgcggc ccgacctcga tctccaccgg ctcggccttc
     2341 gccatggcgt caagctaccc gggacgaggc gggtcgtcag ctgttgtctg ggtatcggcc
     2401 tccggtcagc gttcgcttgc tggccggctc acgcggcctc gacgcaggtt gccggcgtcg
     2461 gacgcagcgc cggacgccgc acggtgcgag cggatcgccg ctcgacctgg gctgcgcgca
     2521 ggtcctgcac ccgtagctgc gtccaggcgt tgcgctgctc ggcctcgacc cgcagccagt
     2581 cgttcatcgt tccccaaggc atggtgctca tgattcgtgg tccttcgtgt tagtgatctc
     2641 ttacattgta agaggcatct aacatagagc gcaagagcag atgtatgatc ggtcttactt
     2701 tcacggcgag gaggcagggt gggctatcac cacggtgacc tgcggactgc gatgatcgac
     2761 gccggcacca ccctcgtggc cgagggcggg ctgcctgcgc tttcggtcgc agaagcggcc
     2821 cggcgcaccg gcgtcagcgc ggccgcgccg taccggcact tcccgacccg gcagcacttc
     2881 ctgatcgcgg tggcagccaa ggctgcgcag gatctggacg cggccatgcg aaccgcagcg
     2941 gacgcggccg gcccggatcc ggtggaagcc atcgccgcag cggcggccgc ttacgtccgg
     3001 ttcgccgcga cccggcgcgc cggcttcgac ctggtttatg cgccggagct gcgtggcgtc
     3061 cacgacgagg agctgcgtga ggctggacgc tccgtcagcg acatcgtgct gctgcgcgcg
     3121 atcgaggcca ccggcggcga cagccgggcc gcggtcgaca tcgtcccgca gttcatgatc
     3181 gtggcccagg gtgcggcgtc catgcatctg atcggcatcc tcagcgacgt cgaatccgcc
     3241 gcccggctgg ccgccaagac gatccgtagc ctgctgcgtt ctgcgccggg ggagtagccc
     3301 tgggtctcgg taggtccaac ttctcggctg gccagacagc ttgcaagatc ctgtaccgac
     3361 ttcggtgcag gatcttgcac gttctgggtt gagggtgatt tcggcgggtg gtgaatcgct
     3421 gggtcaggtg ccgcgcggtg ccggcgcccg gagtcgggct tggcccattc gaagagagaa
     3481 ttcgcttagc atcgctagag ttgcttgtct ggcatctcta gcgatgctaa cctcgttgtc
     3541 tcagctggct gagcacgcga accgggaggg ggctggcatg gctgcggcaa agggcgcgat
     3601 caagggtttc ttccacatct tcaccgccga tcacctcttc acacacgccc ggaacagacc
     3661 tgaagagacc ccgtaccatc tggtcacaat cctggacgtg cttgagccgc agcgtccgag
     3721 acgctgaatc gcaccccgga ttgccagtag ggtgcggact gtgttgacga cctcgcaggc
     3781 cgctgccctg gtgggcctcc ccaaggagca gttccgctcg gcgatgtcca aggagcgcaa
     3841 gaacggcaaa gagttccacg cgcccaagga actgtgggcc gatgcccgca cgccgctatg
     3901 ggacgagaag aaggtgctcg cctgggcgaa ggaccgcaag aagcgcaaga agcgcaagaa
     3961 ggaagaaaag gaagagaagg aagcttccta gcgatcatcc tccggcggag accatcacct
     4021 tgccgagtac cgcaccggcg tccaacgcgg ccaagccgga cgcgacttca gccagcggca
     4081 gcgcccggtc gatccgcggc ttcagccccg ttgcagccac caactcagcg agctgtgcca
     4141 gttcctcgcg ggtccccatc gtcgagccgt gcacgcgcaa ctgcaggaag aacacccggt
     4201 tcagttcggc gggcgggttg tagccggagg tagccccgca aacgacgatc gtgccgcccg
     4261 gcttcagcgc cttcagtgaa tggctccagg tggcttcgcc caccgtttcg atcaccgcgt
     4321 ccacccgttg cggcagccga gcgccggctt cgaacgcggc gtccgcgcca agctcgagcg
     4381 caaaggcgcg tttggcctcg gtgcgtgccg tggcccacac ccgtaggccg agctggcgcc
     4441 ccagctgaat cgctgcggac gcgaccccgc cggacgcacc ctgaatgagg atcgtgtcgc
     4501 ccggggccag gccattgcgc gtgagcatgc ggtaggccgt cagccatgat gttggcagac
     4561 aggcggcatc ctcgaacgag aggttggccg gcttctcgat caggttggcg ttcggtacca
     4621 cgacgtagtc cgccagcgtg ccctggtgca gctcggagag cagcgtccgc ttcgggtcga
     4681 gggtctcgtc gccggaccag tccggcgagc tgatcacggc gtgcacgatc acttccctgc
     4741 cttcgtcggt cgtgccggcc gcgtccgtgc ccagaatcat cggcatgcgc tccggcggta
     4801 gaccgacacc gcgaagcgac cagagatcgt ggtggttcag ggacgccgcg cgcacgcgca
     4861 cccgcgtcca accgtcgggc acctccggct cgggcagttc cccgaccacg agcgcggcca
     4921 atgggtcggc cgggttggcc gctttcacgt acgcagcgcg catgccgcca ctccaggttc
     4981 ctaggcttcg cgggcgagcc tgacccgctc ctgctcggcg atgacggcgc ggtagctgtc
     5041 gcgctcgtgc agcagccagc cgggtgggtt ggcgatcagt tcgccgatct ggtcggcggt
     5101 cagggcgtcc tcgatgccgc cgcgggcgag tcccgatctg gagacgccca gcttctgagc
     5161 gaccacctgg cgcgggaacg gcccgttgcg gcgcaggtcg acgagccact gcggcgggtt
     5221 ggcgcgcagt tcgtccagct cctgccgcgt gatcggggtg cgctggaact cctccggcgc
     5281 cgccggtagg tagatgccga gcttgctcgc cgccgtggct ggcttcatgg tctgactcac
     5341 gagaccccag cgtagtcacg cggacgcgcc tggaccgcct tgagcctggc gaggacgccg
     5401 ccgcccatgg ctcaacttgg ggcgcggcca tcgaaatgag ggctgtcagc ctgctgcagg
     5461 cccgcatagc cctcatttcg gtgcccgagc gggtcggcta gccttcactg gtgaccgggg
     5521 acagtgctca ggcagccgaa gcaacgctgc gggtgggctt tgttcctggg gtgacgctca
     5581 ccaagtggcg acggatctgg ggcgaacggt tcccccgcga gcccctcgaa gtgagcgagg
     5641 tagccgaagc cgagcagcgt cgcgtcctcg tcgaaggcgc tgtcgacctc tgcttcatcc
     5701 ggctgcccat cgagcggacg ggactgcacg cgatcccgct ctacgaagag gtgccggtgg
     5761 tcgtggttcc caaagaccac ccactcgcag ccttcgatga cgtcgagctg gcccagttgg
     5821 cagacctgct cgccgtcgat ccgcaagcac atgacccctt cgacctagtg gcgtacggcc
     5881 agggcgtgct gcgcgtgcca cattcgatcg cgcgctccgg cagccgccgc gacctggttt
     5941 accggccgat catcgacgcc gaaccgacca ccgtcgccct cgcctggcgc atcgacaacg
     6001 ccaacgaact gatcgaggag ttcatcggcg tcgtccgtgg ccggacggtg aacagttccc
     6061 ggaccgccca gtcccgcgcc gcgaacaagg ccgcgaacaa gtcggccggc tcgaaccagg
     6121 taacccaagc gaaccaggac cagcctgtgg cggtccagaa gcgcgccaag gcgaagtcct
     6181 cgacgggccg tcccagcaga cgccgggcgc gacgcccctg agccccggcc caccgtcatc
     6241 ccggcgtagg ccgggatctc agaatgggcc ctggtccacg tcatcccggc gcaggccggg
     6301 atctcggaac gcgttcgaca ggtctgggct gagggttgcc cacgcaaggc atgattgaga
     6361 catgttcgtg aagtcgtctg cagggtcgag aaagtcgctg aagcgcccca tcgcgctgac
     6421 aatcgcggga gcgctggtcg cggccggcct caccggtctg ctggcgagtg gcgcagcagc
     6481 cgagccggtg ctgccaccgc tgaccgcagc cgaactgctc ggcaaggtcg ccgccgccga
     6541 cgtggacggg ctcagcgcca ccttcgagca gcgcagcgac ctcggcctgc ccgcattgcc
     6601 cgccgaactc ggcgacgacg atctcagttc ggtcctggcg ctgctcaccg gcacgcacac
     6661 cgtccgcgtc tgggtggccg gccacgacca ggccaaggcc gcgttgatcg atggcagcac
     6721 cgagagttcg atcacccgca acggtcagga cgtctgggcg tggtcgagcg ccgagcagga
     6781 agctgtccat tccaagctcc cggacgcagc accgtccggt tccagtaccc caacctcgcc
     6841 gaccgccatc attagcgacc tgctgaccaa gctcgaaccc agcaccgagg tggccgttgc
     6901 cagcaccggc tatgtggccg gacgtgcggt ctaccagttg gtgctctcac ctcgcgacga
     6961 ggccagcctg gtgaaccagg tgcgtgtcgc agtggacgcc gaggagttcg ttccgctggg
     7021 ccttcgggtg atcgccgacg acggccagga cgcggtcagc gtggccgcca cttcggttga
     7081 tttcagcgtt ccggacgcgt ccgtattcgc cttccagccg ccggccgggg tgaaggtcac
     7141 cgagaagaag accgacgccg cttcgaccac caagccgggc gacaaggcgt ccgaccgtgc
     7201 cttgccgaag accttcggta gcggctgggc gacggtagcg gtactggacg tgccgaaggg
     7261 cgagacttcc gacgagcagg ccaagattct tctcgattcg ctgccgaagg tgtccggggt
     7321 gtggggtcag ggccggctgt tcagcacctc gctggtgaac gcggtactca ccgacgacgg
     7381 tcgcgtggcg gtcgggtcgg tcgtgccgga gcggctctac gcagccctgc tgaccaagtg
     7441 acgctcgctg ccgaggcggt ggcggacgcg gccgctgacc gcgacggtgc ggcgatcact
     7501 tcgtccgggc tgaccaagcg gttcggcaag caactggtcg tcgacgacct tgccctcgaa
     7561 gtgcccagcg gagcggtcta cggcttcatc gggccgaacg gttccggcaa gaccaccacc
     7621 attcgcatgc tgctcggcct ggtccgtccg gacgccggta cctgcgcgct gctcggcggc
     7681 accatgccgg cggccggcgg cgaagtgctg ccgaaggtcg gggcgctggt cgagggaccg
     7741 gcgttccacc cctacttgtc cggacgggcg aacctgttgc ggctggacgc ggccgatcgg
     7801 cgcagcgacc cggccacagc gtccgcccgg gtggctgccg cgatcgaccg ggtgggcctg
     7861 tccgcagctg ctcgcaagcg gtatcgcgcc tactcactgg gcatgcggca gcgcctcggc
     7921 atcgccgcag cgctgctgca accacgcgaa ctgctggtgc tggacgagcc gaccaacggc
     7981 ctcgatccgc agggcacgcg cgaggttcgg cacctgatcg gcgacctcgc cgccgacggc
     8041 acgacgatct tcgtgtccag ccatctgctg gccgaagtgg agcagctgtg cacccatctc
     8101 ggcgtgatgc gtactggccg cctggtcgct cagggcccga tgcgggagtt gcgggcgctc
     8161 accgccatcc gcttccaggt gcggacgccc gacgcggaac tcgcactggc taccgttgcc
     8221 gccctcggcg tgacggacgc ggcacgcgtc ccagcgggtg tggccggcag cctcggcgag
     8281 gtcgcgcccg agcggatcgt cgccgcgctg gtgaacgcgg gcgtggcggt ggccgagttc
     8341 gcgcaggtga cgccgacgct tgaggacctg ttcgtccagc tgaccgggga gggcttcgat
     8401 gtcagcggct gaggttgcgt ccgcgccctc gcgggtgcgt ccggtggctg cgtgggcgcg
     8461 gctgctggcg tccgagctgc ggctgatctt cggccgccgg cgcaaccagg tcgggctggc
     8521 caccctgttc ggactcgtgg tgctggtggg catcgcgttg aaggtctcgt cgctgcggca
     8581 ccccgggtcg accgggggcg acctgatgtc ggccgtcgcc ggcaacggtt tgttcctcgg
     8641 cttcgccggc ttgagcatcg agatggcgct gttcctgccg ctcgcggtct cggtgctcgc
     8701 cggggacgcg atcgccggcg aggccaacca gggcaccttg cgctacctgc tgctggcgcc
     8761 ggtgagccgc gtccgactac tggtgatgaa ggcgacctcg ctggctatcg gggcgctgac
     8821 cggagcgttc gtggtggccg ccggcggcct gctcaccggc atcgtgctgt tcggcgcgcg
     8881 tccgctgact acgctctcgg gcacctcgat cggcatcgcg gacgggctgt ggcggctcgc
     8941 gctagcggtg ctctacctgg cggcagggct gaccgcgctg gcgatgatcg ggttgttcat
     9001 ctccacgctc accgagcagc cgatcgcggc cacggtcgcc accgcgatgg tcggcacgct
     9061 gctctggatc ctggacgcgg tgccccaact cgactggctg catccgtggc tgctggtcga
     9121 ccgctggccg gcgttcatcg acctgctgcg cgatccggtc ttcaccgatc gcctggtcgc
     9181 cggtctctgg gtggacgccg cctacgccgc cgcgttcttc gccctggcct ggctgcgttt
     9241 ccgtcgcgcc gacatcacca gctgatcgga cgcccgcacg ccggctgagc ctgatgaagc
     9301 ccgccagaac ggtggctgag cctgacgaag ccctcagtca gcgagtcagt gctggtgagc
     9361 catcacataa ccgagcacgc ccggatcgag catgccgaag atcatcgcga cgtgcgccac
     9421 gaccaggaag accgcgatcc agatggcatg ccacttcatc gcctgccggc gccccagcgc
     9481 cacgacaccc agctcgacca gcgtgatgcc ggccaggggg atcaccccgg aaaggtagaa
     9541 gccgaccgcg atcacgtccg ccgggccacg ccagccaccg ctcatggtga gcgggacgac
     9601 cgcgttcagc agcaggtgca gcaggatggc gaccatgact ggaccggcga gcagtccggc
     9661 gatccggctg gtgcggcgca cccactccgg ggcggcctcc cgctggaaca gcaggacgag
     9721 ttcggtgatc gcgagggtct cggcgagaac gaccggcgcg cccatgaaca gcagcaggtt
     9781 ccacggctgg ttggcggcga gcagttccat gtagtgggtc atgcccatgc tcatcgccat
     9841 gccgaggtcg ctggcagcca ggttgggtcc gagcagcgcg gcaaggccga gcaccgcggt
     9901 gagcgcgagg acggtgatcg ctgcagccgg tgagacggat ttggccgcgg cttcggtagt
     9961 ttcgggacgg gtggacgtgg tggtcatggt gatcctcaat tcgggtactc gaccagcctc
    10021 ccgtggcgcc acccagccca gccccggttt caatgaagtt tccatgaagc ttgccgccga
    10081 gcgacgagca atcccaacga acgctccctg aggaggcttt ccgaggaacg agcagtccca
    10141 acggaacgct ccctgaggag gctttccgag gaacgagcaa tccccacgga acgctccctg
    10201 aggaggcttt ccgaggaacg aggaaagccg tcacgaaggg cgtccgccat cccgatccgt
    10261 gagttctacg gaagcactga tcaacgatcc ctgagccggg cgttgggtcg tccattcgct
    10321 ctcggctacg ggcttcgtca agctcagcca gcatcggtcg gcattgatca gcggttccct
    10381 aacgcaccgc agcagagatc tcgacaggct caaccagccg agtcagctcg cgctgcgcgg
    10441 caactccagg gtgaaggtgg cgcctcgacc cacccccgcg ctgactaccg aaagaccgcc
    10501 gccgtgcgct cgggcgatgg cctgactgat cgccagcccc accccggatc cgccatgctc
    10561 acggtcgcgg gccgcgtccg cccggtagaa gcgttcgaag acgtgcggca gatggtcggc
    10621 ggcgatgcct tcgccgttgt ccgcgacttc gatgcgcacc gtggtcgcga gcggacgcac
    10681 ggcaagcgtc acccgacctc cggacggagt gtggcgcagc gagttcgaga gcaggttcgc
    10741 cagcacctga ccgatccggc ccggatccac gtcgaccatc agaccggtgg ccgtcggctc
    10801 ggcaacgagc tcaacttgct tgcgggcgaa ctcctcgcgc gcagcgacgt acgcccggtc
    10861 gacgagagcg ctgaccgc
//
LOCUS       DAOPRQ010000077        10552 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_5287_length_10552_cov_11.834238, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000077 DAOPRQ010000000
VERSION     DAOPRQ010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 10552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10552
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5287_length_10552_cov_11.834238"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..724)
                     /locus_tag="PKJ61_09820"
     CDS             complement(<1..724)
                     /locus_tag="PKJ61_09820"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016667011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HOC14161.1"
                     /translation="MARPKAEPVRPTTMKELKDTLWKAADKLRGSLEASDYKDVILGL
                     VFLKYVSDSFDERRGQIRAELDAEGGYSDDDIEYTLEDADEYSGAGVFWVPPTARWGF
                     LAARAKGVDGITIGTLINEAMGALMDANPALAGTLPRIYNREAVDQRRLGELLDLFNS
                     ARFTGDGATKARDLLGEVYEYFLGKFASAEGKRGGEFYTPPGVVRVLVEMLEPQAGRI
                     YDPCCGSGGMFVQAEKFLEAHHR"
     gene            complement(705..2666)
                     /locus_tag="PKJ61_09825"
     CDS             complement(705..2666)
                     /locus_tag="PKJ61_09825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14162.1"
                     /translation="MAELMTMRDIAGLARVQRPVVTVWRRRARGSVHPFPAAQLHRDG
                     QEFFLRADVVAWLEATQRGNNPDVRADAATHTLLASSGVAEANALSALITLRHLTGEP
                     LGSHTGDELLDLADASDPDDRYLLSELAGADDVASLAAAAEDLVEAAWGELAAHRRLV
                     DARLRPPGSALARLALTEPAGRLLLAIFGPLARELGQPSVMDPTGCAGDVFAEIVAAL
                     ESPVLLMDGDSAAHRLTRRQLLLVDASTRAVGWGVGDWSVTGPVAHLVLLPDAAEPDA
                     SPLEQLAMIDEIALQLDQGQLALCLAPAEVLTDPLSGAALARRDQLLRDGYLRAIVRL
                     PAGLRPAQSREHLALWLLGPADPAQPAERRTLVADVSRLAVRECSGLADDLLAAWQGA
                     DGARRRAWAHLHPILTRDLVSASGTLVPRPPARANRSSRAGADWVVELRSADAAGWLS
                     DYRFEVADGPAALVTLARALEQGWLRMIPGRKLLLDGLPPGNVAVVSGDLVASSPASR
                     LAEAAEITVDRLALLARADVELTEPGDVVFTVRPTPHAVVDPHGGALVLAPARVLRVR
                     PNAPLLPLAIAARINQSTSTDWRGWTVAEFEQPDRDALGEALAELEQRRARLVANLAA
                     VDALTRDLTTAVESRQLRITKENHGPTQG"
     gene            2820..3524
                     /locus_tag="PKJ61_09830"
     CDS             2820..3524
                     /locus_tag="PKJ61_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14163.1"
                     /translation="MSVPQQPTPLGPQPFAPMPGVPPTPPNKRANPLTSKPVIGIVAL
                     LAGMALGSAGGHGGTPAGVSAAVPAATVTATAVQTITAQAEPAEPTEEPTEEATEEPA
                     AEETAAPEPAKKSYASLSKRKFKLLAKDPDSYIGKTYLVYGNVTQFDSATGTDTFRAD
                     AGPKKLRISYGYVDYDQNSIFSGTESKLKKLVEGDCFTAKVTVLGSYSYDTQIGGNTT
                     VPMFAVDSIKVYGSTD"
     gene            3598..3774
                     /locus_tag="PKJ61_09835"
     CDS             3598..3774
                     /locus_tag="PKJ61_09835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14164.1"
                     /translation="MKIPPVAEMMIVARRALHARGIPVTMQLDEDQPVLADDAGRFVI
                     PAGYLVNLVDGSAP"
     gene            complement(4293..4367)
                     /locus_tag="PKJ61_09840"
     tRNA            complement(4293..4367)
                     /locus_tag="PKJ61_09840"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4333..4335),aa:Val,seq:gac)
     gene            complement(4369..4439)
                     /locus_tag="PKJ61_09845"
     tRNA            complement(4369..4439)
                     /locus_tag="PKJ61_09845"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4405..4407),aa:Cys,seq:gca)
     gene            complement(4482..4554)
                     /locus_tag="PKJ61_09850"
     tRNA            complement(4482..4554)
                     /locus_tag="PKJ61_09850"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4519..4521),aa:Gly,seq:gcc)
     gene            complement(4639..5790)
                     /locus_tag="PKJ61_09855"
     CDS             complement(4639..5790)
                     /locus_tag="PKJ61_09855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOC14165.1"
                     /translation="MSGGEVQTSQRVGVEFQVGLGMVNVGFYVASIGVVLVVLAAEFG
                     VPVESLAWLGSLYGVALIVVAFAGPMLLRFGAHRFLAGSAVVLGVGSLLLAVVPGQFA
                     AYAGVILQAFGTSGLLLVTPGLLNGPGAESKLTTVNGAASMVGIAAPLLFGAAQALGI
                     GARLPMLLVVACMAVLTVLALRYPAAIVPNPTHAASGVQRYRRSLVLRRVLTQVSAVA
                     VEFCFVVWGVARLVDTGLGMATAAIVGAAFPVGMAVGRIVGPALIARFPMVPVGSVVA
                     AVGTLAVAATQNPAIVGVGQLVAGLGIATMYPITLARVMATPGLPPELGASLGALGSG
                     VAVMLAPAGLAAVATVVDLRTAFLAPLPLLAIMLLLYRERQPKPGDALA"
     gene            5895..5969
                     /locus_tag="PKJ61_09860"
     tRNA            5895..5969
                     /locus_tag="PKJ61_09860"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5927..5929,aa:Val,seq:cac)
     gene            6207..7001
                     /locus_tag="PKJ61_09865"
     CDS             6207..7001
                     /locus_tag="PKJ61_09865"
                     /inference="COORDINATES: protein motif:HMM:NF014016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HOC14166.1"
                     /translation="MTAPALVMVARGSSDPTVAQVTHQLVRGLAELRPEMAVRGAFLE
                     QSPPSANQVIDQLAANGHHEIVFVPLQLTHAVDCDPAMSDLLHRARVTYPDLRFTLSR
                     PIGPEISLLTVLDQRLRSALSQARCLELDGLVLSASCTGDVRGSALLARRARQWSTHH
                     RLPCLTAVADDSGPSVAQAIMGLRSQGRRHIAVGSFFLSADEAYHAQSDLAYRFGAVS
                     VSDPIGAAREVLDVVLARYAFAAMDLLDFGFEEEIEPARHLSVVGA"
     gene            complement(7148..8020)
                     /locus_tag="PKJ61_09870"
     CDS             complement(7148..8020)
                     /locus_tag="PKJ61_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020384775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HOC14167.1"
                     /translation="MSSQESRKHRSPLSELLRLPSGPVNMADLDAEASPGYPGEGKSD
                     APELTAAIGPELSDLQEMLYAAGRSTPETAPRVLVVLQGMDTSGKGGVIRHAIGLVDP
                     QGVDLTSFKAPSAEERAHHYLWRIKNALPGPGKIGIFDRSHYEDVLVVRVDSLVPESE
                     WSKRYAEINEFEAELVAGGTKLIKCMLNVSLDEQKARLAERLDDPTKHWKYNPGDVDS
                     RQKWPAYMDAYAAALELCNTADAPWYVIPANKKWYRNWAVAELLRETLDGMSLSWPVA
                     DFDVEVEKKRVAES"
     gene            8068..9132
                     /locus_tag="PKJ61_09875"
     CDS             8068..9132
                     /locus_tag="PKJ61_09875"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020384782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HOC14168.1"
                     /translation="MSLPLRPPLEPMLARSSPTIPAGMAYEPKWDGFRCLVFRDNDEV
                     ELISRNGRDLAIYFPEVVAAVLANTPPRCVLDSELVVVQGDRLDFERLSQRIHPAQSR
                     VEMLSNTMPASLVCWDLLALDDTELSARPFRERRELLEGALRHSEPPIHITPITYDLE
                     LAKKWFTQFEGAGLDGVVAKAPDGPYLPGQRAMVKIKHSREADVVVAGWRLHKNSTSE
                     LPLLGSMQLGLFDPDGVLHFVGVAASFPQARRAELAAEFAPLTLPEGATDHPWAASME
                     INGRRPGAISRWSTELKTTLLISPQLVCTVGYEHMEGTRFRHTAQFRRWRSDRDPESC
                     GFDQLEEVVGYDLAEVLGAG"
     gene            complement(9140..10189)
                     /locus_tag="PKJ61_09880"
     CDS             complement(9140..10189)
                     /locus_tag="PKJ61_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HOC14169.1"
                     /translation="MPDATEVQVGERSVRISSPDKVYFADLGLSKLDVVEYFLSVGDG
                     ILAALRDRPTAMERWPGGVREDVVIATRQDHRGEAFYQKRAPANTPDWVQTAQVTFPS
                     GRSAVEVAPADLASIAWMVNLGTVRFHPWPVRAADTDSVDQLRIDLDPQPGVEFSTAV
                     AAALELRSVLAEAGLTGFCKTSGGRGVHVFAPIDGADFIEARHATIAIGRELARRMPQ
                     QVTVNWWKEERGERVFVDFNQMARDRLMTSAYSIRPTPRGLVSAPLDWSELPDADPAD
                     FTLGTMPTRFARIGDPWAGMDATAPGRLDTALEWYDRDAVNGEGEMPYPPEYPKMPGE
                     PPRVQPSKKNEANWA"
     gene            10224..>10552
                     /locus_tag="PKJ61_09885"
     CDS             10224..>10552
                     /locus_tag="PKJ61_09885"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002527295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase"
                     /protein_id="HOC14170.1"
                     /translation="MVEDPRGGTMELKVIKTPEEWRAQLTPLEYRVLRESGTEAPFTG
                     EYTDTTTAGTYRCRACGAELFRSEHKFASHCGWPSFYTAAAEDRVRLLQDNSLPMHPR
                     TEVRCAA"
BASE COUNT         1768 a         3816 c         3360 g         1608 t
ORIGIN      
        1 ttcggtggtg ggcctccagg aacttctcgg cctgcacgaa catgccgccg gagccacagc
       61 acgggtcgta gatgcgtccg gcctgcggtt cgagcatctc gacgagcacc cgaaccacgc
      121 ccggcggggt gtagaactcg ccgccgcgct tgccttcagc cgaggcgaat ttgcccagga
      181 agtactcgta cacctcgccg agcaggtcgc gcgccttggt ggccccatcg ccggtgaacc
      241 gggccgagtt gaacaggtcg agcagttcac caaggcgccg ctggtcgacg gcttcgcggt
      301 tgtagatccg gggcagggtg ccggccagcg ccgggttggc gtccatcaaa gcgcccatcg
      361 cctcgttgat cagggtgccg atcgtgatgc cgtccacacc cttggcccgg gccgccagga
      421 agccccagcg ggcggtcggc ggtacccaga acacgccggc gccggagtat tcgtcggcgt
      481 cctccagggt gtactcgatg tcgtcgtcgc tgtagccgcc ctcggcgtcc agttcggcgc
      541 gaatctggcc acggcgctcg tcgaacgaat ccgagacgta cttgaggaac accaggccga
      601 gaatgacgtc cttgtagtcg gacgcctcca gcgagccgcg cagcttgtcg gccgccttcc
      661 acaaggtgtc cttcaactcc ttcatcgtgg tcggacggac gggctcagcc ttgggtcggg
      721 ccatggttct ccttggtgat gcgcaactgt cggctctcga cagctgtggt gaggtctcgg
      781 gtgagggcgt ccacggcggc gaggttcgcc accaaccgtg cccggcgttg ctccagttcg
      841 gccagcgcct cgccgagggc gtcgcgatca ggctgttcga actcggcgac ggtccagccc
      901 cgccagtcgg tcgaggtgga ctggttgatc cgcgccgcga tcgccagcgg cagcagcggt
      961 gcgttcggac gcacccgcag cacccgggcc ggtgccagca ccagggcgcc gccgtggggg
     1021 tcgacgaccg cgtgcggggt cggtcggacg gtgaacacga cgtcgccggg ctcggtcagc
     1081 tcgacgtcgg cacgggccag cagcgccagc cggtcgaccg tgatctcggc cgcttccgcc
     1141 agccgggacg ccggggagct tgcaaccagg tcgccggaaa ccaccgcgac gttgcccggc
     1201 ggcagtccgt cgaggagcag tttgcgtcca ggaatcatcc ggagccagcc ctgctccagg
     1261 gcccgggcga gggtgaccag cgccgccgga ccgtcggcca cctcgaaccg gtagtcgctc
     1321 agccagccgg cggcgtcggc cgagcgcagc tcgaccaccc agtcggcgcc cgcccgagaa
     1381 ctgcggttcg cccgagccgg cggacgcggc accagcgtgc cgctggccga gaccaggtcg
     1441 cgggtcagga tcggatgcag gtgcgcccag gcccggcgac gggccccgtc cgccccctgc
     1501 caggcggcca gcaggtcatc ggccagcccg gaacactccc gcacagcgag gcggctcacg
     1561 tcggcgacca gggtgcgccg ctcggcgggc tgggccggat cggccggccc gagcagccac
     1621 agcgccaggt gctcgcgcga ttgggctgga cgcaggccgg ccggcaaccg gacgatcgcc
     1681 cgcaggtagc cgtcgcgcaa gagctggtcg cgccgggcca acgcagcgcc cgacagcgga
     1741 tcggtgagca cctcggccgg ggccaggcac agcgccaact gtccttgatc gagttgcagg
     1801 gcgatctcgt cgatcatcgc caactgctcc agcggggatg cgtccggctc ggccgcgtcc
     1861 ggcaacagca ccaggtgcgc gaccgggccg gtgaccgacc agtcgccaac gccccacccc
     1921 actgcccgtg tcgaggcgtc caccagcagc agctggcgcc gggtcagccg gtgggcagcg
     1981 gagtcgccgt ccatcagcag caccggcgac tccagcgccg ccacgatctc ggcgaacacg
     2041 tccccggcgc agccggtcgg gtccatcacg ctcggctgtc ccagttcgcg cgcgagcggg
     2101 ccgaagatcg ccagcagcag tcgcccggcc ggctcggtga gcgccagtcg ggccagcgcg
     2161 ctccccggcg gacgcagccg ggcgtccacc agccgccggt gggcggccaa ctctccccag
     2221 gccgcctcca ccaggtcttc ggcggccgcg gccaacgagg ccacgtcatc ggccccggcc
     2281 agctcgctca acaggtagcg atcgtcggga tcggacgcgt cggccagatc gagaagctca
     2341 tcgccggtgt gggaaccgag cggttcaccg gtgagatgcc gcagcgtgat cagggcgctc
     2401 agcgcgttgg cttcggcgac cccgctggac gcgagcaacg tgtgcgtcgc cgcatcggcc
     2461 cggacgtcgg gattgttgcc gcgctgggtc gcttcgagcc acgccaccac gtcggcgcga
     2521 aggaagaact cctggccgtc gcggtgcagt tgggccgcgg ggaacggatg gacgctgccg
     2581 cgggcccggc gtcgccacac cgtgaccacc ggacgctgca cgcgcgccag gccggcgatg
     2641 tcgcgcatcg tcatcagctc agccatcgca gcctcccgtc caggacattc caccactcac
     2701 cgctgaccct tcgcgggcga atggtgcgat aacccccctt atcgacgcat cgctgtcctt
     2761 cgggagcgct tgcgcgacac cgtggtggcc agcaggagtc gcatcgaaag gaatcgacca
     2821 tgtccgtccc gcagcagccc accccgctgg gcccccagcc gttcgcacca atgccgggag
     2881 tgccgccgac gccgccgaac aagcgtgcca atccgctcac ctccaagccg gtgatcggca
     2941 tcgtggcgtt gctcgccgga atggctctcg gctcggcagg tggccacggc ggaacccccg
     3001 ccggagtgag tgcagcggtg ccggcggcca ccgtcaccgc gaccgccgtc cagacgatca
     3061 ccgcccaggc cgaaccggcc gagccgaccg aagagccgac cgaggaagcg accgaagaac
     3121 ccgccgccga ggaaaccgcg gcacccgagc cggccaagaa gagctacgcc tcactgagca
     3181 agcgcaagtt caagctgctg gccaaggatc ccgactccta catcggcaag acctatctgg
     3241 tctacggcaa cgtcacccag ttcgactcgg ccaccggcac cgacaccttc cgggccgacg
     3301 ccggaccgaa gaagctgcgg atctcctacg gctacgtcga ctacgaccag aactcgatct
     3361 tcagcgggac ggagtcgaag ctgaagaagc tggtcgaggg cgactgcttc accgccaagg
     3421 tgaccgtgct ggggtcctac tcctacgaca cccagatcgg cggcaacacc accgtgccga
     3481 tgttcgcagt ggactcgatc aaggtctacg gctcgaccga ctgatcagaa ccagaccggt
     3541 gtgggctgat cagggggaat cggcccacac cccgcttcca cggagaagga tgcactcatg
     3601 aaaatcccgc cggtagccga aatgatgatc gttgctcgac gagccctgca cgctcgcggc
     3661 attccggtca cgatgcagct cgatgaagac cagccggtcc tggcggacga cgccggacgc
     3721 ttcgtgattc ccgcgggcta cctggtgaac ctggtggacg gcagcgcgcc ctaggattgg
     3781 ccggacgtct tcgcggcgct ggccgacaac gtgtccgcct cgctgaccca ggctcatccg
     3841 gaggatctga gcgcggatca gctccgagct cagattcgca cccgcctggt tgcgaccagc
     3901 gaggtcgcgt ccgcagacct gacgcctcaa tgcgaaggca cgatcacacc aaggggtagg
     3961 tggtgttctg gacggttgcc gcagttcggc ggcgccgtcc gcgaagcccg cacccaaacc
     4021 ggcctgaccc atcgggaact cgccgaccgc gcgaacgtct cccgcccttg gctcggaaac
     4081 accccagtgt cgctatccat gccgctggtc cagaccgaac agggaccgtg ccgtcgagcc
     4141 gttcctcgac gggctgcttc ccgaccgaga ccacgcgccc ggcgaaaccg attcagcggc
     4201 ccggcgcggc agagagccgc atctcgatgt gcccagcttt cgacccacaa cagcacaaga
     4261 gcccccaatc agattctgac tagggacttc gttggtgggc gacactggga tcgaaccagt
     4321 gacctcttcc gtgtcaggga agcgcgctac cgctgcgcca gccgcccgag gtggagacgg
     4381 gatttgaacc cgtgtacacg gatttgcagt ccgttgcctc gcctctcggc cactccaccg
     4441 gcgagagccg gcctcgtctt cgatgcttga gaccttctcc gagcggacga cgggattcga
     4501 acccgcgacc ctcaccttgg caaggtgatg ctctaccaac tgagccacgt ccgcatgctc
     4561 ccggcccgtt tccggcccaa gtgcgagtgc aaccataacc cagccgcgaa gatgccgcaa
     4621 aacgagggcg cggacgagtc aagccagcgc gtcgcccggc ttgggctgcc tctcccgata
     4681 cagcagcagc atgatcgcga gcagcggcag cggcgccagg aaggccgtcc gcagatcgac
     4741 caccgtcgcg accgcagcca gccccgccgg cgccagcatc accgccaccc cggagccgag
     4801 agcgcccagg gacgcgccca gttccggcgg aagccctggc gtggccatca cccgcgcgag
     4861 cgtgatcgga tacatcgtcg cgatgcccag ccctgcgacc aactggccca cacccacgat
     4921 cgccggattc tgtgttgccg ccaccgccag cgtgccgacc gcggccacca ccgagcccac
     4981 cggcaccatc gggaaccgtg cgatcagggc tgggccgacg atccgtccga cagccatgcc
     5041 cactgggaaa gcggccccca cgatcgcggc ggtggccatg cccaagccgg tgtccaccag
     5101 ccgcgcaacg ccccacacca cgaagcagaa ctccaccgcc accgcactca cctgcgtcag
     5161 cacccgccgc agcaccaggc tgcgccgata ccgctgaaca ccggacgccg cgtgtgtcgg
     5221 gttcggcacg atcgccgccg gatagcgcag cgcaagcacg gtcaaaacag ccatgcatgc
     5281 caccaccagc agcatcggca gccgggcgcc gatccccagc gcctgcgccg ctccgaacag
     5341 caggggtgcg gcgatgccga ccatgcttgc cgccccgttc accgtggtca gcttggactc
     5401 cgcacccgga ccgttgagca gccccggcgt gaccagcaac agccccgatg tcccgaacgc
     5461 ctgcaaaatc acgccggcat aggccgcgaa ctggcccggc accaccgcca acaggagact
     5521 ccccacaccc agcaccaccg ccgagccggc gaggaagcgg tgcgcgccga accgcagcag
     5581 catcgggccc gcgaacgcga cgacgatcag cgcgacgccg tacagcgaac ccagccaggc
     5641 caggctctcg accggaaccc cgaactcggc agccaacacc accaggacca ccccgatgct
     5701 ggccacgtag aagcccacat tgaccatgcc gagcccgacc tggaactcaa cgccgacgcg
     5761 ctgggacgtc tgcacttcac cgcctgacac ttgccactcc tcgccgaatc cgaaatcgct
     5821 tcccgctgcc ggttcccgcg accaactcgc cgattgtgcg tcaccaccgc cgctgggcta
     5881 tggttgttcc ccgcgggcga ttggcgcagt ggtagcgcgc ttcgttcaca ccgaagaggt
     5941 cactggttcg aacccagtat cgcccaccat cccgcgcctg ctttccactt gatggaaagc
     6001 ggattcccat tccctgagac aagtcctggg ttttgcggcc cggttttgga ctcatcgcca
     6061 ggctaaatgg atatctccgt ctctccatat ggacaatcgt ctcatcattc ggtaagcaga
     6121 attgcgtcac aacttgataa cggacagagt tctgcctaaa gaacctcggc aatgaagccc
     6181 ggtcccttcg gacaggaagg caagccatga ccgcccccgc actagtgatg gtggcccgag
     6241 gtagttctga ccccacggtg gcgcaggtca cccaccagct cgtccgcggc ctcgctgagc
     6301 tgcgtccaga gatggccgtc cgcggcgcct ttctcgagca gagcccgccg agcgcgaacc
     6361 aggtcatcga ccagctggcg gcgaacggac accacgagat cgtcttcgtc ccgttacagc
     6421 tcactcacgc ggtggattgc gatcccgcga tgtcggacct gctgcaccgg gcccgagtca
     6481 cgtacccaga cctgcgcttc acgctctcgc gtccgatcgg cccggagatc agcctgctga
     6541 ccgttctcga ccagcgcctg cgcagcgcgc tcagccaggc tcgctgcctg gaactggacg
     6601 ggctggtgct ttctgcatcc tgcaccggcg acgtgcgcgg cagtgccctg ctggcgcgac
     6661 gcgcccggca gtggtcgacc caccaccggc tgccctgcct cacggccgtc gcggacgatt
     6721 ccggcccaag cgttgcccag gcgatcatgg ggctgcgctc ccagggccga cgccacatcg
     6781 cggtcggctc cttcttcctg agcgcggacg aggcctacca cgcccagtcc gatctggcgt
     6841 atcgattcgg cgccgtctcg gtgagcgatc cgatcggagc cgctcgcgag gttctggacg
     6901 tggtcctggc ccgctacgcc ttcgccgcca tggatctgct cgacttcggt ttcgaggaag
     6961 agatcgagcc cgctcggcac ctgagcgtcg tcggcgcctg agtctttcga cccacccggc
     7021 ccgctcggct ggccactagt ccctgcgatc ggaacttcgc tcccgaatgg gacccgtgct
     7081 cccgataatt caggagcgcg agtcccattc gggagcgaag ttgcctacgg acgggcagac
     7141 gaccggctca gctttcggcg actcgcttct tctcgacctc gacatcgaaa tcggcgaccg
     7201 gccagctcag gctcattccg tccagagtct cgcggagcag ctctgcgacc gcccagttgc
     7261 ggtaccactt cttgtttgcc ggaatgacgt accacggcgc gtccgcggtg ttacagagct
     7321 ccagtgcggc cgcgtacgcg tccatgtagg cgggccactt ctggcgagaa tccacgtcac
     7381 cggggttgta cttccagtgc ttggtcggat cgtcgagccg ctcggccaac cgagccttct
     7441 gctcgtccaa ggacacgttc agcatgcact tgatcagctt ggtgccaccg gccaccagtt
     7501 cggcctcgaa ctcgttgatc tcggcgtacc gcttgctcca ctcggattcg gggacgaggc
     7561 tgtccacccg caccacgagc acgtcctcat agtgcgaacg gtcgaagatg ccgatcttgc
     7621 cgggtccggg caacgcgttc ttgatccgcc aaaggtagtg gtgggcccgt tcctcggcgc
     7681 tcggcgcctt gaaggaggtc aggtccaccc cttgcggatc caccagcccg atcgcgtggc
     7741 gaatcacccc gcccttaccg gacgtgtcca tgccttggag caccacgagc accctcggcg
     7801 cggtctcggg cgtactgcgt ccggctgcat agagcatctc ctgcagatcg gacagctccg
     7861 gaccgatcgc agcggtcagt tctggagcgt ccgacttacc ctctcccgga tagccggggc
     7921 tggcttccgc gtccaggtcg gccatgttca ccggcccgct gggcaggcgc agcagctccg
     7981 atagtgggct gcgatgcttc cttgattcct gggaactcat tgccacagtc tggaccgaat
     8041 agccaccttt ggtgccaaga ttggcgaatg tcgctgccac tacgtccgcc gctggaaccg
     8101 atgctggccc gctcgagccc caccattccc gctggcatgg cctacgagcc gaagtgggac
     8161 gggttccgct gcctggtctt ccgggacaac gacgaggtgg agctgatcag ccgcaacggc
     8221 cgggacctgg ccatttactt ccccgaggtg gtggccgctg tcctcgccaa tacgccgccg
     8281 cgctgcgtcc tggattccga actggtggtg gtgcagggcg accggctcga tttcgaacgt
     8341 ctctcccaac gcatccatcc tgcccagagc cgcgtcgaga tgctgtcgaa cacgatgccc
     8401 gcatccctgg tgtgctggga cctgctcgca ctcgacgaca ccgaactcag cgcgcggccg
     8461 ttccgcgaac gtcgcgaact tctggaaggg gccctgcgcc acagcgagcc gccgattcac
     8521 atcacaccga tcacctacga cctggagctc gccaagaagt ggttcaccca gttcgagggc
     8581 gccgggctgg acggcgtggt cgccaaggca ccggacgggc cctacctgcc cggacagcgc
     8641 gcgatggtca agatcaagca ttcgcgcgag gcggacgtgg tggtggccgg ctggcgcctg
     8701 cacaagaact ccacctcgga gctgccgctg ctcgggtcga tgcagctcgg cctcttcgac
     8761 ccggacgggg tgctgcactt cgtcggcgtg gccgcatcct tcccgcaggc tcgccgggcg
     8821 gaattggccg ccgagttcgc cccgctgacc ctgccggaag gcgccactga tcatccctgg
     8881 gcagccagca tggagatcaa cggacgccgg cccggtgcga tcagccgctg gagcaccgag
     8941 ctcaagacca cgctgctgat ctcgccgcag ctggtctgca cagtcggcta cgagcacatg
     9001 gagggcaccc ggttccgcca caccgctcag tttcggcgct ggcgctccga ccgtgatccc
     9061 gagtcctgcg gcttcgatca gctcgaagag gtggtcggct acgacctcgc cgaggtcctg
     9121 ggcgccggct gagagtcggt cacgcccagt tcgcttcgtt cttcttcgag ggctgcaccc
     9181 ggggcggctc gcccggcatt ttcggatact cgggcgggta gggcatctcg ccttcgccgt
     9241 tcacggcgtc gcggtcgtac cactccagcg ctgtgtccaa gcgtccaggc gcggtcgcgt
     9301 ccatcccggc ccacgggtcg ccgatccttg cgaagcgggt cggcatggtg ccgagggtga
     9361 agtcggccgg gtcggcgtcc ggcagttcgg accagtccag gggcgccgag accaggcctc
     9421 gcggcgtcgg tcggatcgag taggccgagg tcatcaaccg gtccctggcc atctggttga
     9481 agtcgacgaa gacccgctcc ccgcgctcct ccttccacca gttcaccgtc acctgctgcg
     9541 gcatccgccg cgccagttcg cgtccgatcg cgatcgtggc atgccgtgcc tcgatgaagt
     9601 ccgcgccatc gatcggggcg aacacgtgga cgccgcgtcc gccgctggtc ttgcagaacc
     9661 cggtcagacc agcctcagca agcaccgaac gaagctccag cgctgccgcg accgcggtcg
     9721 agaactcgac tccgggctgc gggtccagat cgatccgcag ctggtcgacc gaatcggtat
     9781 ccgccgcccg caccggccag gggtggaacc gcacggtacc aaggttgacc atccacgcga
     9841 tgctcgccag gtcggccggc gccacctcga ctgcgctgcg cccggaagga aaggtgacct
     9901 gagcggtctg cacccagtcc ggcgtgttgg ccggcgcacg cttctggtag aacgcctcgc
     9961 cccggtggtc ctgccgggtg gcgatcacca catcctcgcg gacgcctccg ggccagcgct
    10021 ccatcgcggt cggacggtcc cgcaacgcgg ccaggatccc gtccccgacg ctgaggaagt
    10081 actccaccac gtccagcttg ctcaagccca gatcggcgaa gtagaccttg tctggactcg
    10141 agatccggac cgatcgctcc cctacttgga cttcggtggc atcaggcatt tccgaaggct
    10201 acaaggtgtc ggcggctacg cctatggtgg aggacccaag aggaggaacg atggaactga
    10261 aggtgatcaa gaccccggaa gagtggcgcg cgcagctcac gccgctggaa taccgggtgc
    10321 tccgcgagtc gggcaccgag gcgccgttca ccggggagta caccgacacg accacggccg
    10381 gcacctatcg ctgccgagcg tgtggcgccg aactgttccg cagtgagcac aagttcgcat
    10441 cccactgcgg ctggccgtcg ttctacaccg cggccgccga ggaccgcgtc cgcctgctgc
    10501 aggacaacag cctgccgatg cacccgcgta ccgaggtgcg ttgcgcggcc tg
//
LOCUS       DAOPRQ010000078        10551 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_5289_length_10551_cov_11.506383, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000078 DAOPRQ010000000
VERSION     DAOPRQ010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 10551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10551
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5289_length_10551_cov_11.506383"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..907
                     /locus_tag="PKJ61_09890"
     CDS             <1..907
                     /locus_tag="PKJ61_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002514992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HOC14171.1"
                     /translation="QDIYPINFDNDPEGIYHETLRVLKLWIAHGVRIFRVDNPHTKPV
                     QFWAWLMRQVRATDPDVLFLAEAFTKPAMMGALGKVGFHQSYTYFTWRNAKWELEEYL
                     TELSTKTDPFYRPNFFVNTPDINPFFLQSGNPAAFSIRAILAATLSPTWGVYSGFELF
                     EHEALAPGKEEYLNSEKFEYRPRDYQREPNLNVLLGKLNQVRRDHPALQQLRQLQFQH
                     APHDNVIAFAKRDGDDAVIVICSLDPNQVVETDIYLDLDWLGVPGDSVQLHDELTGAT
                     FTWGERNFVRLSPANPAHILHVRGH"
     gene            918..2228
                     /locus_tag="PKJ61_09895"
     CDS             918..2228
                     /locus_tag="PKJ61_09895"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HOC14172.1"
                     /translation="MTTPNAAWADYLRQCRWFGGKGLAADVTSIEPLPWYTPPGSWPA
                     VRSELATVSYADGRTETYQLLVAYVPADSSAPGVAVGSTELPDLGAVDFLDAPADPAA
                     MTALVHALRERAVDGMKWLDPEPVDAEAEMRLYTGEQSNTTVNVGGGALFKLFRKLEP
                     GRNLDAEVLAALTGSGITPAAYGVLASPDCGYDLALFLERVPEVTDGWVYATAACASG
                     RDFSTEARALGTELRELHRRLADTFGTATTNGDALSAAMLARFDAAAAGVRELADHRH
                     AARLIFAAAKGTQLVTQRIHGDFHLGQVLHRHAEPSWVIIDFEGEPLKSLAERREFDS
                     VWRDVAGLLRSLDYARSAHAEPDGREALAWCASAKNAFLEGYVGGPASASELLRAYEF
                     DKAVYEVVYEVRNRPDWAHIPWRGVQDEVMLATLEASATKTEEH"
     gene            2228..4126
                     /gene="glgB"
                     /locus_tag="PKJ61_09900"
     CDS             2228..4126
                     /gene="glgB"
                     /locus_tag="PKJ61_09900"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015070616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HOC14173.1"
                     /translation="MAHDLAGGLTGWDLEGFHNGGDTECWRRLGAHEVTLFDDERGEI
                     RGTRFAVWAPNAREVRLNADFNWWTGDSMHLVPGSGVWGLFVEGVGTGALYKFNILGP
                     DGVWREKVDPMAFFAEQAPANSSIVYTSKYAWADDGWMDRRAKAEPYKEAMSIYEVHL
                     GSWRRGKTYLELADELVEYVTWQGYTHVELMPVTEHPFEPSWGYQVTNYFAPQSRLGR
                     PDELRYLIDRLHQAGIGVILDWVPGHFPKDEWALGRFDGTALYEHADPRQGEHRDWGT
                     YIFNYGRNEVKSFLVSNALYWVTEFHIDALRVDAVASMLYLDYSREDGEWVPNIHGGN
                     ENLEAIDFLRYVNKHLYERVPGVVMIAEESTSFGGVTKPVHSGGLGFGFKWNMGWMND
                     SLRYLALEPIHRQYHHNEMTFAMVYAYSENFILPISHDEVVHGKGSMINKIPQDTWRQ
                     FATLRAFYSYMWAFPGKKLIFMGCEFGQRSEFNESASLEWWVSDLWGHKGLQRMFKDL
                     NELYRSHPALWELDSDPAGFRWINADDAPANTFSWVRSDSSGRQVAVAVNFSSEPWTR
                     YRIGLPKAGVWKEIFNSDSATYDGSDAHGNLGQVVATDEGWNGMPASAIVVVPPLGAV
                     YFEHVESE"
     gene            4123..4905
                     /locus_tag="PKJ61_09905"
     CDS             4123..4905
                     /locus_tag="PKJ61_09905"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOC14174.1"
                     /translation="MIRDSLPFSIADGVAVIDFRGDDGPERYELLLTGTCQQVFDTDG
                     VRRAEVNICAADAPRRRALHRAGFRLEGVLRERLLTASGPADVLCYARLATDEAAGRT
                     GFTAVMNTVTPRKRLISHMLLTDESGRVCILETTFKPDFELPGGILDVGETPRQGLVR
                     EIEEELNHTMSVGRLLVVDWLAPYLGWEDAVELIFDGCEMPLASVGLLRPDLREIRAV
                     HWLEPDAAAERMAPFARGRLLAALAARAEGRTLYLEAGQRIS"
     gene            5181..7685
                     /locus_tag="PKJ61_09910"
     CDS             5181..7685
                     /locus_tag="PKJ61_09910"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOC14175.1"
                     /translation="MSLTAQPAPASAPARRLGVLTAVYLLGIFMGAIDTGIVTPARTI
                     IQNDLGVNEQTGIWMLTIYTLAYAAAIPVMGKLADRYGRKPVYLASITLFGVGSLLCG
                     LSQDFGSFELLLIARAIQAIGGGGIVPIATAEFGTSFPPEKRGMALGLVGGVYGVANI
                     FGSSAGSLILDIFGTANWQFIFYVNVPISIAIIIAGLVFLPNHKVEQVAKIDLLGITL
                     LVAMILSLLYGLRNLDFFDFANSVTSTDVYPYLIGFLVLLPLFILAERRAADPVVNLR
                     YFTEGALVVTLALSFLSGVILMGVIFVPQFAENALRIPSGDGGYFVIILGLFSGIGAP
                     LSGRLTDRFGPNLVLGFGVVMSAAAAAAGVWWAIPHASWLSVCTTLALMGLGLGFTIG
                     SPLNYMMLERTPAEESNSALATLSLVRSIGTTIAPAFLVGFLAQAGTALPDALTAQLP
                     TTVAAPALPYAAELQAKFAELKADERLEDRLAGVEFPDLTSATTIDIGTMDGSATLPD
                     DLLELVQTADVTNITERTKVVAEHMFAEQTPGVIADITAGVDEGLASLADAHTELTHA
                     RTKLVDAVAGIDEGIAGMERAAKGMRSGIAGLTKAISGMDSGLAGTAKAIKGMEKAIA
                     GMTKGVKGLDHAIAGQQRGIAQLQAAYDQLMATLPPGTPEPPPAQAMRAQLETLKAKL
                     ADTKKSRSSLVAKRNTLEANLKKVRAERKQLTAQRASAVSKRAKLREQLTGVEAKRAS
                     AVKDRKKLVQARDDLSTAMTELAETKRQLTVLCAAVPGAFETAKADYLAEIDARAPKL
                     EQTFAATLNVGFRGIYLATLGASLLAAVLLLLRRGR"
     gene            complement(7689..7952)
                     /locus_tag="PKJ61_09915"
     CDS             complement(7689..7952)
                     /locus_tag="PKJ61_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14176.1"
                     /translation="MTSSRRSLLPRALWLAFGCLVLVSYVTGLVAGFRADELAGAPPA
                     PAGLDLAALSTITWLTVGLTVVVGIMALVMTVSRRNAGSAPRI"
     gene            complement(7962..8930)
                     /locus_tag="PKJ61_09920"
     CDS             complement(7962..8930)
                     /locus_tag="PKJ61_09920"
                     /inference="COORDINATES: protein motif:HMM:NF022240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2599 domain-containing protein"
                     /protein_id="HOC14177.1"
                     /translation="MIRTRTLIATIAAAMALAVPQSPMAVADTADPVESLADQVAAAI
                     ETSEPVISPTRGGADLVAESANGSAQVALALDAEEISLTDLKSGTSVAIGLPVEAEVE
                     RPAVAQDGTVIYQESGEGGVDIAAQVIADGSIRVQTIISDRTSPHEFSYPLTLPAGTI
                     LELTDDGGVLASDAHGGFVAGVRPPWAKDSQGNDVATAYRIEGNTIVQTVAESDARVY
                     PVVADPWLGIDLIEKVTRVWDSAKKGYRYSVFPTWWGRVGASRLARGDAWSETKKKGV
                     PQTSTLENQFLCHYDNRVLVAFKGSWNLEAYTKDRGYWGFMANLCN"
     gene            complement(9156..10043)
                     /locus_tag="PKJ61_09925"
     CDS             complement(9156..10043)
                     /locus_tag="PKJ61_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015070621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOC14178.1"
                     /translation="MTSAGFHPSRAMDLSGIVAAAKAPPPPSGATYVLEVDERNFEAV
                     IGGSVKYPIVLELTSPRANAQALSDALTELANEAGGAYLLARVNVDASPQIAAALGIQ
                     AVPAVIGVVAGQLAPLWQGTKDKAEAKAYLAQLLQAAAANGVTGRAEPASIDADAGPD
                     PRFAAADAALASGDFETAVAEFDKLLAASPNDTEAKAGRAQAALLSRAAKLDPAQALA
                     AADANPTDVAAQLAAADAELLGGAIPSAFGRLIALIRDSTAAEREQARVRLLELFETV
                     APDDPAVLKARRDLVSALF"
     gene            10295..>10551
                     /locus_tag="PKJ61_09930"
     CDS             10295..>10551
                     /locus_tag="PKJ61_09930"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002548824.1"
                     /note="catalyzes the interconversion of alpha-D-glucose
                     1-phosphate to alpha-D-glucose 6-phosphate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase, alpha-D-glucose
                     phosphate-specific"
                     /protein_id="HOC14179.1"
                     /translation="MAHERAGQVALPEDLIDVDALLRAYHDLVPDPDNPDQQVVFGTS
                     GHRGSALDAAFNDAHIAATTQAIVEYRASQGVTGPLFIGKDT"
BASE COUNT         1837 a         3573 c         3418 g         1723 t
ORIGIN      
        1 ccaggacatc tacccgatca acttcgacaa cgatcccgag ggcatctacc acgagacgct
       61 gcgcgtgttg aagctgtgga tcgcgcacgg cgtccggatc ttccgggtgg acaacccgca
      121 caccaagccg gtgcagttct gggcctggct gatgcggcag gtgcgcgcga ccgatcccga
      181 cgtgctgttc ctcgctgaag ccttcaccaa gccggcgatg atgggcgcgc tgggcaaggt
      241 gggcttccac cagagctaca cctacttcac ctggcgcaac gccaagtggg agctcgagga
      301 gtacctgacc gaactgagca cgaagactga cccgttctac cggccgaact tcttcgttaa
      361 cacccccgac atcaacccgt tcttcctgca gtcgggcaac ccggcggcgt tctcgatccg
      421 ggcgatcctg gctgccacac tctccccgac gtggggggtg tactccggct tcgaactttt
      481 cgagcacgag gcgctggcac cgggcaagga ggaatacctg aactcggaga agttcgagta
      541 ccggccgcgc gactaccagc gcgagccaaa cctgaacgtg ctgctgggca agctcaacca
      601 ggtacggcgc gaccacccgg cgctgcagca gttgcggcag ctgcagttcc agcacgcgcc
      661 gcatgacaac gtgatcgcgt tcgccaagcg ggacggcgac gacgcggtga tcgtgatctg
      721 cagcctcgat cccaaccagg tggtggagac cgacatctac ctcgacctgg actggttggg
      781 ggttccaggc gacagtgtgc agctgcacga cgagctgacc ggcgcgacct tcacctgggg
      841 cgagcgcaat ttcgtccggc tcagcccggc gaacccggcg cacatcctgc acgtacgcgg
      901 gcactgaaag cgcaccggtg accactccga atgccgcatg ggcggactac ctgcggcagt
      961 gccgctggtt cggcggcaag ggcctggccg cggacgtgac gtcgatcgag ccactgccct
     1021 ggtacacgcc gccgggtagc tggcccgcgg tgcggtccga actggccacg gtcagctacg
     1081 ccgacggtcg cacggagacc taccagttgc tggtggcata cgtgcccgcg gacagttcgg
     1141 cgccaggggt cgcggtcggc agcacggagc tgcccgacct cggcgcggtc gacttcctgg
     1201 acgcaccggc tgatccggcg gccatgaccg cgctggtgca cgcactgcgg gaacgcgcgg
     1261 tggacggcat gaaatggctt gacccggaac cggtcgacgc cgaggcggag atgcggctct
     1321 acacgggtga gcagagcaac accacagtga acgtcggcgg cggagccctg ttcaagctgt
     1381 tccgcaagct cgaaccggga cgcaatctgg acgctgaagt gctcgccgcg ctcaccggtt
     1441 cgggcatcac cccagcggcg tacggcgtcc tggcctcccc ggactgcggg tacgacctgg
     1501 ccctgttcct ggagcgggtg cccgaggtca ccgatggctg ggtctacgcg accgctgcct
     1561 gcgcgtccgg acgcgacttc agcaccgaag ccagggcgct cggcaccgaa ctccgcgaac
     1621 tccaccgccg gctggcggac acgttcggca ccgccaccac gaatggcgac gcgctgagcg
     1681 cggcaatgct ggcccgtttc gacgctgccg ccgctggagt ccgcgaactg gccgaccacc
     1741 ggcacgcagc ccggctgatc ttcgccgcag ccaagggcac ccaactggtc acgcagcgga
     1801 tccacggcga cttccatctc ggtcaggtgc tgcaccgcca cgcggagccg agctgggtga
     1861 tcatcgactt cgaaggcgag ccgttgaagt cgctggcgga gcggcgcgag ttcgattcgg
     1921 tctggcgcga tgtcgccggg ctgctgcgca gcctcgatta cgcccgcagc gctcacgccg
     1981 agccggacgg acgcgaggcg ctggcctggt gcgcgtccgc caagaacgcc ttcctcgaag
     2041 gctacgtcgg cgggccggcg tccgcgtctg agctgcttcg ggcctacgag ttcgacaagg
     2101 cggtctacga agtcgtctat gaggtgcgca atcgacccga ttgggcacat atcccctggc
     2161 ggggggtcca agatgaggtc atgttggcca cacttgaggc gtccgctacg aaaaccgagg
     2221 agcactgatg gcacacgatc tggctggcgg actaaccggt tgggaccttg agggtttcca
     2281 caacggcggc gacactgagt gctggcgacg cctcggcgcc cacgaggtca cgctcttcga
     2341 cgacgaacgc ggcgagatcc gcggcacccg cttcgcggtc tgggcgccga acgcgcgcga
     2401 ggtgcgactg aacgccgact tcaactggtg gaccggcgac tcgatgcacc tggtcccggg
     2461 atccggcgtc tgggggctgt tcgtcgaggg cgtgggcacc ggcgcgctgt acaagttcaa
     2521 catcctcggc ccggacgggg tctggcgcga gaaggtcgac ccgatggcgt tcttcgccga
     2581 acaggcgccg gccaactcgt ccatcgtcta caccagcaaa tacgcctggg ccgacgacgg
     2641 gtggatggat cgccgcgcca aggcggagcc gtacaaggag gcgatgagca tctacgaggt
     2701 gcacctgggc tcctggcggc gcggcaagac ctacctcgaa ctagccgacg aactggtgga
     2761 gtacgtcacc tggcagggct acacccacgt ggaactcatg ccggtcaccg agcacccgtt
     2821 cgagccgtcc tggggctacc aggtcaccaa ctacttcgcg ccacagtcgc ggctgggccg
     2881 tcccgatgag ttgcgctacc tgatcgaccg gctgcaccag gccggcatcg gcgtgatcct
     2941 cgactgggtg cccggccact tccccaagga cgagtgggcg ctgggacgct tcgacggtac
     3001 ggccctgtac gaacacgccg acccccgcca aggcgagcac cgggactggg gcacgtacat
     3061 cttcaactac ggccgcaacg aggtgaagtc cttcctggtc tccaacgcgc tgtactgggt
     3121 gaccgagttc cacatcgacg cgctgcgggt ggacgcggtc gcgtccatgc tctacctgga
     3181 ctactcccgc gaggacggcg agtgggtgcc gaacatccac ggcggcaacg agaacctgga
     3241 ggccatcgac ttcctgcgct acgtgaacaa gcacctctac gagcgggtgc cgggcgtcgt
     3301 gatgatcgcc gaggagtcca cctcgttcgg tggggtgacc aagcccgtcc attccggcgg
     3361 cctggggttc gggttcaagt ggaacatggg ctggatgaat gactcgctgc gctacctcgc
     3421 gctggagccc atccaccggc agtaccacca caacgagatg acgttcgcca tggtgtacgc
     3481 gtactcggag aacttcatcc tgccgatcag ccacgacgag gtcgtgcacg gcaaggggtc
     3541 gatgatcaac aagattccgc aggacacctg gcggcagttc gccaccctgc gcgcgttcta
     3601 ctcctacatg tgggccttcc ccggcaagaa gctgatcttc atgggctgcg agttcggcca
     3661 gcgctcggag ttcaacgagt cggccagcct ggagtggtgg gtctccgacc tgtgggggca
     3721 caagggcctg cagcggatgt tcaaggacct caacgagctc taccgcagcc atccggcttt
     3781 gtgggagctg gattccgatc cggccggctt ccgctggatc aatgccgacg acgcgcccgc
     3841 gaacaccttc agctgggtgc gcagcgactc gtccggtcgc caggtcgcgg tcgcggtcaa
     3901 cttctcctcc gaaccctgga cgcgctaccg catcgggctg ccgaaggccg gcgtgtggaa
     3961 ggagatcttc aactccgact ccgccaccta cgacggctcg gacgcccacg gcaacctcgg
     4021 ccaggtggtg gccaccgatg agggctggaa cggcatgccg gccagcgcga tcgtcgtggt
     4081 gccgccgctg ggcgcggtct acttcgagca cgtcgaatcg gagtgattcg ggactcgctg
     4141 ccgttcagta ttgcggacgg ggtggccgtc atcgacttcc gcggtgatga cggcccggag
     4201 cgctacgaac tgctgctgac cggcacctgc cagcaggtct tcgatacgga cggggtccgg
     4261 cgggccgaag tgaacatctg cgctgcggac gcgccccgcc ggcgggcgct gcaccgggcc
     4321 ggcttccggc tcgaaggggt gctgcgggag cgactgctca ctgcttccgg gccggccgat
     4381 gtgctctgct acgcccggct ggccaccgac gaggccgccg ggaggaccgg gttcaccgcg
     4441 gtgatgaaca ccgtgacccc gcgcaagcgg ctgatcagcc acatgctgct caccgacgag
     4501 tccggccggg tgtgcattct cgagaccacc ttcaagcccg acttcgagct gcccggcggc
     4561 atcctggacg tcggtgaaac tcctcgccaa ggcctggttc gcgagatcga ggaggagctg
     4621 aaccacacca tgagcgttgg ccggctgctg gtggtggact ggctcgcgcc ctacctcggc
     4681 tgggaggacg cggtcgagct gatcttcgac ggctgcgaga tgcctttggc ttcggtgggg
     4741 ctgctgcgtc ccgacctgcg cgagattcga gccgtccact ggctcgaacc ggacgccgcc
     4801 gccgagcgga tggctccgtt cgcccgcggc cgcctactgg ccgccttggc cgcccgagcc
     4861 gaaggccgca cgctctacct cgaagccggc caacggatca gctgacataa gcggtccctg
     4921 aggagcgagc ccagccagcg ccccgaacgg aactcccgct cccgagagtt cgggagcagc
     4981 ggtcccgttc gggagcgaag ttggcattgc ggtgcttgag ggagttcccg gcgcgcccat
     5041 taactgtggc tccacccacc gtccgcggtg cacccagcca cagtcaaggc cgcacagctc
     5101 cggcacaacc ttcgacaggc acccgccgac ccagaagccg gtcactatag ttcgcttgag
     5161 actaactaac cggagcgatc ttgagcctga ccgcccagcc agcacccgcc agcgcgcccg
     5221 cgcgccgcct cggcgtgttg accgcggtct acctgcttgg cattttcatg ggcgcgatcg
     5281 acaccggcat cgttaccccc gcgcggacaa tcatccagaa cgacctcggc gtgaacgagc
     5341 agaccggcat ctggatgctc accatttaca ccctcgctta cgccgcggcg atcccggtga
     5401 tgggcaagct ggccgaccgc tacggccgca aaccggtcta tttggccagc atcaccctct
     5461 tcggcgtcgg gtcgctgctt tgcgggctca gccaggactt cggcagtttc gaactgctgc
     5521 tcatcgcgcg ggcgatccag gccatcggcg gcggtggcat cgtcccgatc gcgaccgctg
     5581 agttcggcac gtccttcccg ccggagaagc ggggcatggc tctcggcctg gtcggcggcg
     5641 tgtacggcgt ggcgaacatc tttgggtcga gcgcaggcag cctcatcctg gacatcttcg
     5701 gaaccgcgaa ctggcagttc atcttctatg tcaacgtgcc gatcagcatc gcgatcatca
     5761 tcgccgggct ggtcttcctg cccaaccaca aggtcgaaca ggtcgccaag atcgatctgc
     5821 tgggcatcac gctgctggtc gcgatgatcc tgtccctgct ttacggcctg agaaacctcg
     5881 acttcttcga cttcgccaac agtgtcacga gcaccgacgt ctacccgtac ctaatcggtt
     5941 tcctcgtgct gttgccgctg ttcatcctcg ccgagcgccg ggcggccgac ccggtggtta
     6001 acctgcgcta cttcaccgag ggcgcgctgg tggtcaccct tgcgctctcg ttcctgtccg
     6061 gcgtgatcct gatgggggtc atcttcgtac cccagttcgc cgagaacgcg ctgcggatcc
     6121 cgtccggtga tggcggctac ttcgtgatca tcctcggcct gttctcgggg atcggcgcgc
     6181 cactttcggg acgcctgacc gaccggttcg ggcccaatct cgtgctgggc ttcggcgtgg
     6241 tgatgtcggc tgcagccgcg gcagccggag tgtggtgggc cattccgcac gcgtcctggt
     6301 taagcgtctg cacaaccctg gcgctgatgg ggctcgggtt gggcttcacc atcggctcgc
     6361 cgttgaacta catgatgttg gagcggacgc cggccgagga gtccaactcg gcactggcca
     6421 cgctgtcgct ggtccgctcg atcggcacga ccatcgcgcc ggcgttcctg gtcggcttcc
     6481 tcgcgcaggc cggcactgcc ctgccggacg ccctaactgc ccagctaccg accaccgtgg
     6541 ccgccccggc actcccctac gccgccgaac tgcaggccaa gttcgccgag ttgaaggcgg
     6601 acgaacggct ggaggaccgg ctggccgggg tcgagttccc tgacctgacc agcgcgacca
     6661 cgatcgacat cggcacaatg gacgggagcg cgacgctgcc cgacgacctg ctcgaactgg
     6721 tgcagaccgc cgacgtgacc aacatcaccg agcgcaccaa agtggtggcc gagcacatgt
     6781 tcgctgagca gacgcctggc gtgatcgccg acatcaccgc gggcgtggac gaagggctcg
     6841 ccagcctggc ggacgcgcat accgaactga ctcacgctcg caccaagctg gtggacgcgg
     6901 tcgccggcat cgacgagggc atcgccggaa tggagagagc agccaagggc atgcgctctg
     6961 ggatcgccgg gctcaccaag gcgatcagcg ggatggactc gggtctggcg ggcaccgcga
     7021 aggccatcaa gggcatggag aaggccatcg ccggcatgac caagggcgtc aaggggctcg
     7081 accacgccat cgctggccag cagcggggga tcgcccagct gcaggccgcc tacgaccagc
     7141 tgatggccac gctgccgccg ggcacgcccg agccacctcc tgcccaggcg atgcgggcac
     7201 agctggagac gctcaaggcc aagctcgctg acaccaagaa gtcgcgctcg tcgctggtgg
     7261 ccaagcggaa cacgctggag gcgaacctca agaaggtgag agccgagcga aagcaactga
     7321 ctgcgcagcg agcgtccgcg gtgagcaagc gcgcgaagct gcgcgaacag ctgaccggcg
     7381 ttgaggcgaa gcgcgccagc gcggtgaagg accgcaagaa gctggtgcaa gccagggacg
     7441 acctgagcac cgcgatgacc gaactggccg agaccaagcg tcagctgacg gtgctgtgcg
     7501 cggccgtccc cggggccttc gagaccgcca aggccgacta cctcgctgag atcgacgcgc
     7561 gcgctcccaa gctggagcag accttcgccg ccacgctgaa cgtgggcttc cgcggcatct
     7621 acctggccac tctcggcgcc agcctgctgg cggccgtgct gctcctgctc agaagagggc
     7681 gctgactatc agattcgcgg cgcgctcccc gcgttcctgc gcgacacggt catgaccaac
     7741 gccatgatgc ccactacgac ggtcaagccc acggtcagcc aagtaatggt ggacagtgcg
     7801 gccaggtcga gcccggccgg cgcaggtggc gcgccggcga gttcatcggc gcgaaaaccg
     7861 gcgaccaatc cagtcacgta actcaccaac accaggcagc cgaacgcgag ccatagtgcc
     7921 ctgggtagca acgatcgtct tgaactcgtc atatcgccag cttagttgca gagattcgcc
     7981 atgaagcccc aataccctct gtccttcgtg tacgcctcaa ggttccacga gcccttgaac
     8041 gccacgagaa cccggttgtc gtagtggcac aggaactggt tttctagagt ggacgtttgg
     8101 ggaacaccct tcttcttggt ctctgaccat gcatcccccc gcgccaagcg actcgcccca
     8161 acgcgacccc accaggtggg gaagactgaa tagcggtagc ccttcttcgc gctatcccag
     8221 acacgtgtga ccttctcgat gaggtcaatc ccgagccacg ggtcggctac aacagggtag
     8281 acacgggcat ctgattcggc cacggtctga acgatcgtgt tgccttcgat tcgataagcg
     8341 gttgctacgt cgttcccttg agagtccttt gcccaaggcg ggcgcacccc agcaacgaaa
     8401 cctccatggg cgtcggaggc gagcactccg ccatcgtcgg tcaactccaa gatcgtcccg
     8461 gctggcagtg tcagcggata gctaaactca tgcggagagg tcctgtccga gatgatcgtc
     8521 tggactcgaa tcgagccgtc cgctatcacc tgagcggcga tatcaacgcc cccctctccc
     8581 gattcttggt agatgacagt cccgtcctgg gcgacggcgg ggcgctcgac ttcggcttcc
     8641 accggcaacc caatcgccac agacgttccg ctcttaaggt ctgtcaagga gatctcctca
     8701 gcatcaagtg caagcgcaac ctgcgctgaa ccattagccg actcggccac gagatccgcg
     8761 ccaccacgcg ttggagagat caccggctca ctggtctcga tcgctgcggc cacctgatca
     8821 gccaacgact cgacaggatc tgcagtatca gcaacggcca tcggtgactg aggtacggcc
     8881 aacgccattg ctgcagcgat cgtggcgatg agggtgcgtg ttcggatcat gagcgtccta
     8941 atctcggcgg taggtagata gccaccgaaa ctagtgcaat tcaatgttcg aggctcatgt
     9001 atttcaccac aaagtgtacg acaactcttc tgtccgcttg cccccagccc gagagcgcgc
     9061 aggcctcacg ggcttgtccc cagtcgagcc taaatcgaac ccgacgagac cctccgtagg
     9121 cactggagtc ggcgctctct gccagcccat gccagtcaga agagggcgct gaccaggtcg
     9181 cgccgcgcct tcagcacggc cgggtcgtcg ggagccacgg tctcgaacag ttcgagcagg
     9241 cggacgcgag cctgctcccg ctcagctgcg gtgctgtcac ggatcaaggc gatcaaccgt
     9301 ccgaacgcgg acgggatcgc accgcccagc agttcggcgt ccgctgcggc cagttgcgcc
     9361 gccacatcgg tcgggttggc gtccgccgcg gccagcgcct gggctggatc gagcttcgcc
     9421 gcacgcgaca gcaaggcagc ctgggcgcgt ccggccttgg cttcggtgtc attcgggctg
     9481 gccgcgagca gcttgtcgaa ctcggccacg gcggtctcga aatcgccgga ggcgagtgct
     9541 gcgtccgccg cggcgaatcg cgggtccggc ccggcgtctg catcgatgct ggccggttcg
     9601 gcgcggccgg tgaccccgtt ggccgcggcg gcctgcagca actgcgcgag ataggccttg
     9661 gcctcggctt tgtccttcgt accctgccaa agtggcgcga gctgaccggc caccacaccg
     9721 atgaccgccg gaaccgcctg gatgccaaga gcggccgcga tctggggcga cgcgtccacg
     9781 ttgacccgag ccagcaggta cgccccgccg gcctcgttgg cgagctcggt gagcgcgtcc
     9841 gacagcgcct gcgcgttggc ccgcggcgag gtgagttcga gcacgatcgg gtacttcacc
     9901 gagccgccga tcaccgcttc gaagttgcgc tcgtccactt cgagcacata ggtcgcgccc
     9961 gacggtggcg gcggcgcctt ggcggcggcg acgatgcccg atagatccat ggcgcgggac
    10021 gggtgaaagc cagctgaagt cacaagcaag cattctgcca gccgtaccga agcggtgcga
    10081 atgtggtccg gcggttcccc actgtcgtac gtaggagcct gctgaacaat catgtcccgg
    10141 ctgcggcgca ctgggcgggc gatctgctgc ggccacatct tgggtcggta ggcccactac
    10201 agaccccaga tgtggccttg cagctcatcc cgcccagcac gtcctcgctc ggaacagcat
    10261 tgttcagcag actcctaggc ggcaggatgg ccccatggca cacgaacgcg ccgggcaggt
    10321 tgcactgccc gaagacctga ttgacgtcga cgccctgctc agggcctacc acgacctggt
    10381 gcccgatccg gacaaccccg accagcaggt cgtgttcggc acgtccgggc atcgcggctc
    10441 cgcgctcgac gcggccttca acgacgcgca catcgcggcc acgacgcagg cgatcgtcga
    10501 gtaccgggcc agccagggcg tcaccggtcc gctgttcatc ggcaaggaca c
//
LOCUS       DAOPRQ010000079        10457 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_5359_length_10457_cov_11.783022, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000079 DAOPRQ010000000
VERSION     DAOPRQ010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 10457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10457
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5359_length_10457_cov_11.783022"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="PKJ61_09935"
     CDS             complement(<1..245)
                     /locus_tag="PKJ61_09935"
                     /inference="COORDINATES: protein motif:HMM:NF022668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3017 domain-containing protein"
                     /protein_id="HOC14180.1"
                     /translation="MTTPTDPRPPKTFMQQVLGQWPLAGVLTGVALGLVVVAFGHWRA
                     GSTLIGAAITAGGLLRLLPQTRVGLLAVRSRAIDSILL"
     gene            complement(242..1093)
                     /locus_tag="PKJ61_09940"
     CDS             complement(242..1093)
                     /locus_tag="PKJ61_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016455504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HOC14181.1"
                     /translation="MTARILDGRAVAATIKGELAMRVAALESRGVRAGLGTVLVGDDP
                     GSRSYVAMKHRDCAEIGIESIRVDLPADASAERVAAAITGLNENPACTGYIVQLPLPR
                     HLNENAMLELIDPDKDADGLHPINLGKLVLNEPAPLPCTPRGIVELLRRHDVPLAGAE
                     VCVVGRGVTVGRPIGLILTRRSENSTVTLCHTGTRDLAAHTRRADIVIAAAGVPGLIS
                     AAMIAEGAVVLDVGVSRVDGKLVGDVAADVLQKASWVAPNPGGVGPMTRAMLLSNVVD
                     RAEALAG"
     gene            complement(1102..2661)
                     /gene="purH"
                     /locus_tag="PKJ61_09945"
     CDS             complement(1102..2661)
                     /gene="purH"
                     /locus_tag="PKJ61_09945"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016669476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HOC14182.1"
                     /translation="MNDLLPIRRALVSVYDKTGLIELGQALVASGVELVSTGSTASTL
                     ANAGLPVTPVEQVTGFPECLDGRVKTLHPHIHAGILADRRLESHRQQLDELGVAPFDL
                     VVSNLYPFTQTVASGAAVGECVEQIDIGGPSMVRAAAKNHPSVAIITSPAQYAELTAA
                     LAAGGFTLAQRQALAAAAFVHTATYDVAVASWMGSVLTDSSGGDGFPAWIGGTWDKVG
                     TLRYGENAHQGAALYAQPSGPAGLTQAEQLHGKEMSYNNYTDGDAAYRAAYDFTESAV
                     AIIKHANPCGIAVGADVAEAHRKAHACDPVSAFGGVIATNREVSVELAEQVAEIFTEV
                     IIAPGYADGAVEVLARKKNVRVLVAPPYPQRSVQLLPISGGALLQRPDRIDAPGDDPA
                     NWQLVAGEAADAATLADLEFAWRACRAVKSNAILLASGGASVGVGMGQVNRVDSCKLA
                     VERAGERARGSVAASDAFFPFADGPAILIEAGVRAIVQPGGSVRDAETIAAAEAAGVT
                     MYLTGTRHFFH"
     gene            complement(2658..3233)
                     /gene="purN"
                     /locus_tag="PKJ61_09950"
     CDS             complement(2658..3233)
                     /gene="purN"
                     /locus_tag="PKJ61_09950"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002546166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HOC14183.1"
                     /translation="MPKRLVVLVSGSGTLLQALLDASAAGELRAVVAAVGADRPDAYG
                     LTRAEAAGVPTFVHPLTKGADRAAWDAELTGLVAAHEPELIISAGFMKLVGPAFLARF
                     GGRMINTHPALLPSFPGMHGVRDALAHGVKVTGATVFLVDEGVDSGRILAQEAVPVLP
                     DDTEEVLHERIKIVERRLLVDTVNELCQGDA"
     gene            complement(3263..4501)
                     /locus_tag="PKJ61_09955"
     CDS             complement(3263..4501)
                     /locus_tag="PKJ61_09955"
                     /inference="COORDINATES: protein motif:HMM:NF040070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6350 family protein"
                     /protein_id="HOC14184.1"
                     /translation="MPSPKRRATERVQFSVSSARTVRIPVTDAPPLFPWPVAAIGGGL
                     LAGLAGSLLLAGVVMVAWFSAIAIELPAVLAFAAQSWLLAHGGVLPIGGSQITLVPLG
                     LTFLFGALCAAIGGFAYRQGRQARTGEPTRGQQTALVLGSIGQVAAGYLAYTVLVAWT
                     LAGTAGILRPALGALAVSVVGAGIGALTASGQRLDAIGPSWVRRSIRGAAAGMLGLII
                     AGSVALGTAMLLGETRIAALEAGLKFDNGGLFVWSLVALAYLPNLLVWSLAWVLGGGF
                     TVGTGSLVSLWTTQLGMLPAVPVFGALPPAGVASDWLLAWLVLGVSAGAVAGVVVASK
                     GRTSVVGVLGSAVLAGLLAALGYLAWAAAGRGDLGILRLVDLGPRLTESAVIGAPLLV
                     LSAAVGGLVAWFVRRRRSRA"
     gene            4606..6435
                     /locus_tag="PKJ61_09960"
     CDS             4606..6435
                     /locus_tag="PKJ61_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14185.1"
                     /translation="MTAENSSTPRRALAPLDDDTTGNARYGFADFGDGIDDDEDAGEN
                     TVVLRRLAMSPLSPDSAGSPASPAELSAGDPIPARASAATPASASSAGDPLDVESTTP
                     VPPPMPVLPDPEAALPPASGRRFSASTEPLEYAWAAPRRSAASPLSPTSPNPAIEPAI
                     PPAPSRPTAPPSVTVDALTATRADAAEPAKAAQSKTKQRNRKHLLIIFGALAAFAVLV
                     SGVVYLLTLDSIAPSGAPTTSATAAALDPLVTPEDLGNLAGLDWASGITTADGARPIC
                     LPAESDGLPEAQRSVNRKITVGTAAVVNVVDTYADVATATAAYQIRLAEAGTCPETTA
                     LITGASSISGLADSADAVRVTVQNEANEYHSLQLTRTGRTVGLLDVITSTDDVNAAAV
                     ASASAASQNRQCTGSEGTCPSSISVAADVPAAGALPGWLVAADLPRITPAAGRWGATD
                     PATTLHLIGSQCEVIDLNAVTGTTSVGQRTFLLADDAKAPSGFGIDQAVYSFSAAKAA
                     TNLRDKLAKNIDGCPDRAPTAKVTEGPSIKGTGVNGAKIAGSTYLVTQSTGTTTVAFR
                     VAVLTVDTRMVYLLANPSSSFDFSDDDWRQLAVRAGQRVSQSS"
     gene            complement(6500..6730)
                     /locus_tag="PKJ61_09965"
     CDS             complement(6500..6730)
                     /locus_tag="PKJ61_09965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14186.1"
                     /translation="MTEPEGVCRMSVDGELWDVSHRGDGQYDLSWTRENSPNYGFSMN
                     LGGREAEMTPQILEDVIREFMDNINPETGYLD"
     assembly_gap    7064..7163
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7453..7584)
                     /locus_tag="PKJ61_09970"
     CDS             complement(7453..7584)
                     /locus_tag="PKJ61_09970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14187.1"
                     /translation="MDYSTDLRPISCSTDPLAGTDGQFARQASSSLKSKLLDGLASK"
     gene            7654..8898
                     /locus_tag="PKJ61_09975"
     CDS             7654..8898
                     /locus_tag="PKJ61_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016456866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HOC14188.1"
                     /translation="MTWYVPRIIDAQAERAVASAGAVVFRGPRAVGKTESALRLAASE
                     LRLDSADPRAALARTQPGSALDGATPRLLDEWQVVPELWNEVRHAVDDRRASGQFIMT
                     GSAWPDDDRLLHSGAGRFRQVTVRTMSLAETGESTSAVSLAGLFEGDLRMAESATDFR
                     GVVQRLVRGGWPGWLDADEPAAIARATSYVTDIAEHDFVTVAGPRRDPRRLVAFLRAL
                     AGLTAQPASYAAITRRMNEKFTNIGAAAVPELHDLAQRLYLVEDQPAWSPSLRSRTAL
                     LQTPSRHLADPSLAAALLSAGTDRLLSEPPTLGFIFESQVVHDLRVYAQAADSRGVFH
                     YRDAKARDEIDAVVEASDGRWLAVEVKLGPAQVDAAAANLLRVTAKVSRPPSACVVVT
                     PSGVAHRRPDGVMVVPLTVLGP"
     gene            complement(8946..9833)
                     /gene="sucD"
                     /locus_tag="PKJ61_09980"
     CDS             complement(8946..9833)
                     /gene="sucD"
                     /locus_tag="PKJ61_09980"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020576915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HOC14189.1"
                     /translation="MSIWLDQRSKVIVQGMTGAEGRKHTQRMFGAGTRIVGGVTPGKG
                     GQSVVFEEDPVPVFDTVADAKAATGANTSVVFVPAKFARAAVIDAVDANLDLTVVITE
                     GIPVKDTAEFVAYARQQGHTRLIGPNCPGLISPGKSNVGIIPPNIAGAGPIGLVSKSG
                     TLTYQMMFELRDIGFSTAVGIGGDPVNGSTHIDVLAAFEDDPDTKAIVLIGEIGGDAE
                     ERAAAYIAEHVTKPVVGYVAGFTAPVGKTMGHAGAIVTGSAGTARAKKAALEASGVRV
                     GRTPSETAALLREIVAGLG"
     gene            complement(9846..>10457)
                     /locus_tag="PKJ61_09985"
     CDS             complement(9846..>10457)
                     /locus_tag="PKJ61_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002516025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit beta"
                     /protein_id="HOC14190.1"
                     /translation="LVEVNPLVKTGSGQIVALDGKVSLDANSAFRHPEWADYADESSD
                     DPLEVKARAKGLNYVKLDGSVGVIGNGAGLVMSTLDVVAAAGADLPGTPRPANFLDIG
                     GGASAQVMANGLDIILSDPQVKVVFVNVFGGITACSEVAKGIIDALAILGDRATRPIV
                     VRLDGNAVEIGRHMLAEAAHPLVELVSTMDEAARRAAELAAAE"
BASE COUNT         1696 a         3700 c         3413 g         1548 t
ORIGIN      
        1 agcaggatcg agtcgatcgc tcggctgcgc acagccaaca ggccgacccg ggtctggggc
       61 agcagccgca gcaggcctcc ggcggtgatg gccgcgccga tcagggtgct accggcccgc
      121 cagtgtccga acgcgaccac caccaggccg agagccacgc cggtgagcac tccggccagc
      181 ggccattggc cgagcacctg ctgcatgaag gtcttcggcg gacgcgggtc ggtgggcgtg
      241 gtcaccctgc caatgcttcc gcacggtcga ccacattgga aagcagcatc gccctggtca
      301 tgggcccgac accaccgggg ttcggcgcca cccacgacgc cttctgcaga acgtcggccg
      361 ccacatcgcc gaccagcttg ccgtcgaccc ggctgacgcc cacgtcgagc accaccgcgc
      421 cctcggcgat catggccgcc gagatcagcc cgggcacacc cgctgcggcg atcacgatgt
      481 ccgcgcgccg ggtgtgtgcg gccaggtccc gcgtcccggt gtgacacaac gtcaccgtgg
      541 aattctcgct gcgccgggtg aggatcagcc cgatcggccg tcccacggtg acaccacgac
      601 ccaccacgca cacctcggcc ccggccagcg gcacgtcgtg gcgccgcagc agttcgacga
      661 tcccgcgcgg cgtgcacggc agcggcgccg gctcgttcag caccagcttg cccaggttga
      721 tcggatgcag cccgtccgcg tccttgtccg gatcgatcag ttcgagcatc gcgttctcgt
      781 tcaggtgccg cggcaggggc agctgcacga tgtagccggt gcaggccggg ttctcgttca
      841 gcccggtgat cgcggcggct acccgctcgg cggacgcgtc cgcgggcagg tcgacccgga
      901 tcgactcgat gccgatctcg gcgcaatcgc ggtgcttcat ggccacgtag ctgcgcgaac
      961 cggggtcgtc gccgaccagc accgtgccca ggccggcccg gacgccgcga gattccagcg
     1021 cggccacccg catcgcgagt tcacccttga tcgtggccgc taccgcccgg ccgtccagga
     1081 ttcgtgcagt cataccgagc ctcagtggaa gaagtgacgg gtgccggtga ggtacatcgt
     1141 gaccccggca gcctcagcgg cagcgatggt ctccgcgtcc cgcaccgagc cgcccggctg
     1201 cacgatcgcg cgcaccccgg cctcgatcag gatcgccggc ccgtccgcga acgggaagaa
     1261 cgcatcggac gcggccaccg aaccccgcgc gcgctccccg gcccgctcga ccgcgagctt
     1321 gcaggagtcg acccggttca cctggcccat gcccaccccg accgacgccc caccggacgc
     1381 cagcaggatc gcgttcgact tcaccgcccg gcaggcccgc caggcgaact cgagatcggc
     1441 gagcgtggcc gcgtccgctg cctcgccggc aaccaactgc cagttcgccg ggtcgtcgcc
     1501 gggggcatcg attcgatccg gacgctgcag cagcgcacca ccggagatcg gcagcagttg
     1561 cacgctgcgc tgcggatacg gcggcgccac cagtacgcgc acgttcttct tgcgggccag
     1621 cacttcgacg gccccgtccg cgtagccggg ggcgatgatc acctcggtga agatctcggc
     1681 cacctgctcg gccagttcga cgctcacctc acggttcgtg gcgatcacgc cgccgaacgc
     1741 cgagaccggg tcgcaggcgt gggctttgcg atgcgcctcg gcgacgtccg cgccgaccgc
     1801 gatgccgcac gggttggcgt gcttgatgat cgccaccgcc gattcggtga agtcgtaggc
     1861 cgcacggtag gccgcgtcac cgtcggtgta gttgttgtag ctcatttcct taccgtgcag
     1921 ctgctcggcc tgggtgagcc ccgccggccc ggacggttgg gcgtacagcg cggccccctg
     1981 gtgcgcgttc tcgccgtagc ggagcgtgcc gaccttgtcc caggtgccgc cgatccatgc
     2041 cgggaagccg tcgccgccgg acgaatcggt aagcaccgag cccatccagg acgccaccgc
     2101 gacgtcgtag gtggcggtgt gcacgaaggc tgcggcggcc agagcctgcc gctgcgccag
     2161 ggtgaatcca cccgcagcca gggccgcggt gagttcggcg tactgcgctg gtgaggtgat
     2221 gatcgccacg gacggatggt tcttggccgc ggcccgcacc atcgacggcc cgccgatgtc
     2281 gatctgctcc acgcactcgc cgacggcggc acccgaagcg acggtctgcg tgaacgggta
     2341 aaggttcgag accaccaggt cgaacggcgc tacgcccagc tcgtcgagtt gctggcgatg
     2401 ggactccagc cgccggtcgg ccaggatgcc ggcgtggatg tgcggatgca gggtcttcac
     2461 ccggccgtcc aggcattcgg ggaacccggt cacctgctcc accggtgtca ccggcaaacc
     2521 ggcgttggcc agggtcgatg cggtcgaccc agtcgagacc agctcaactc ccgaggccac
     2581 cagtgcctgg ccgagttcga tcaggccggt cttgtcgtag accgaaacca gggcgcggcg
     2641 gattggcagc aggtcgttca tgcatcccct tggcagagtt cgttgacggt gtcgaccagc
     2701 aggcgccgct cgacgatctt gatccgttcg tgcaggactt cttcggtgtc gtccggcagc
     2761 accgggacgg cttcctgggc caggatccgg cccgaatcga cgccttcgtc aaccaggaac
     2821 acggtcgcgc cggtcacctt cacaccgtgc gccagcgcgt cccggacgcc gtgcatgccg
     2881 gggaaggacg gcagcagggc cgggtgggtg ttgatcatgc ggccgccgaa gcgggcgagg
     2941 aacgccggac cgaccagctt catgaagccg gcgctgatga tcagttcggg ctcgtgggcg
     3001 gccacgagcc cggtcagttc cgcgtcccag gcggcgcggt cggcgccctt ggtcagcgga
     3061 tgcacgaagg tgggcacgcc ggcggcttcc gctcgggtca gcccgtaagc gtccggacga
     3121 tcggcgccga cggccgcaac caccgccctc aactcgccgg cggcggacgc gtccaggagt
     3181 gcctgcagca gggtgccaga gcccgagacc aggacgacca ggcgcttcgg catgcggtcg
     3241 agcctatcga accccggacg gctcaagccc gagagcgcct acggcggacg aaccacgcca
     3301 ccagcccgcc gaccgccgcg ctgagcacca gcaacggcgc cccgatcacg gcgctttccg
     3361 tcagtcgggg cccgaggtcg accagccgca ggatgccgag atcaccccgg ccggccgcag
     3421 cccaggccag gtagccgagc gcggccagta gtccggccag cacggcggaa ccgagaacgc
     3481 cgaccacact cgtccgtcct ttgctcgcga ccaccacccc ggcgacggct ccggctgaaa
     3541 cgcccagcac cagccaggcc agcagccaat cggacgcgac cccggccggc gggagggctc
     3601 cgaagaccgg gacggccggc agcatgccca actgggtcgt ccacagcgac accagcgacc
     3661 cggtgcctac cgtgaacccg ccaccgagca cccaggccag cgaccacacc aacaggttcg
     3721 gcaggtaggc cagcgcgacc agcgaccaga cgaacaggcc gccgttgtcg aacttgagtc
     3781 cggcctccag cgcggcgatc cgggtctcgc ccagcagcat cgcggtgccg agagccacgc
     3841 tgccggcgat gattagcccg agcattccag cggccgctcc acgtatcgag cgacgtaccc
     3901 agctcggccc gatcgcgtcc aatcgctgtc cggacgcggt cagggccccg atgccggccc
     3961 cgacgacact caccgcgaga gcgcccagcg ccggccgcag gatgccggcc gttccggcga
     4021 gcgtccaggc caccagcacg gtgtaggcga ggtagccggc agcgacctgt ccgatcgagc
     4081 ccagcacgag agcggtctgc tggccgcggg tgggctcgcc ggtgcgggcc tggcgtccct
     4141 ggcggtaggc gaagcctccg atcgcagcgc agagcgcacc gaacaggaag gtgagcccga
     4201 gcggcaccag ggtgatctgg ctgccaccga tgggcagcac accaccgtgt gcgagcagcc
     4261 aggactgggc ggcgaaggcg agcaccgcgg gtagttcgat ggcgatcgcg ctgaaccagg
     4321 cgaccatcac cacacccgcc aggagcagcg aaccggccag cccggccagc agcccgccgc
     4381 cgatcgcggc caccggccaa gggaagagcg gcggcgcgtc ggtgacaggg attcgcaccg
     4441 ttcgagcgct ggagacgctg aactggacgc gttcggtcgc acggcgtttg ggactaggca
     4501 tggtctctcc atgctgaccc accagttgcg cgaccgaagg tgggacacgc cagcggatgg
     4561 ctagactggc taggtcgtct ccggaacggt caagggaaag caagcgtgac agcagagaat
     4621 tcgtccacgc caagaagggc gctggctccg ctcgacgacg acacaaccgg taacgcgcgc
     4681 tacggcttcg ccgacttcgg cgacggtatc gacgacgacg aagacgccgg cgagaacacg
     4741 gtagtgctgc gccgcctggc catgagtccg ctttcgccgg acagcgccgg cagccccgcg
     4801 agccctgccg agttgagtgc aggcgacccc attccggcca gggcatctgc ggccacgccc
     4861 gcgagcgcga gcagcgccgg tgatccgctc gacgtggagt cgaccacccc cgtcccgccg
     4921 ccgatgccgg tgctgcccga tccggaggcc gcactgccgc ccgcgtccgg acgccggttc
     4981 tcggcctcca ccgaaccgtt ggaatacgcg tgggccgccc ctcgccgctc ggcagccagc
     5041 ccgctctcgc cgacttcacc gaacccggcg atcgaaccgg caatcccgcc ggcgccgagt
     5101 cgcccgaccg ccccgcccag cgtgaccgtg gacgcgctca cggcaaccag ggcggacgcg
     5161 gcggagccgg ccaaggcggc gcagtcgaag accaagcagc gcaatcgcaa gcatctgctg
     5221 atcatctttg gcgcactggc cgcgttcgcg gtgctggtgt ccggggtggt ctacctgctc
     5281 acgctggact cgatcgctcc gagcggtgcg ccgacgacaa gtgccaccgc ggcagccctc
     5341 gacccgctgg tcacccccga agatctgggc aacctcgccg gcttggactg ggcttccgga
     5401 atcacgaccg cagatggcgc gcggccgatc tgcctgcccg ctgaaagcga cggcctgccc
     5461 gaagcgcagc gcagcgtgaa ccgcaagatc accgttggca ccgcagcggt ggtgaacgtg
     5521 gtcgacacct acgcggatgt ggcgaccgcg accgcggcct accagattcg gctggccgag
     5581 gccggcacct gtccggagac caccgcgctg atcaccggcg cgagttcgat cagtgggctc
     5641 gccgattcgg ccgacgccgt ccgggtgacc gtgcagaacg aggcgaacga ataccactcc
     5701 ctccaactca cccggaccgg ccggacggtc ggcctgctcg atgtgatcac cagcaccgac
     5761 gatgtgaacg ccgccgcggt ggccagcgct tccgcggctt cgcagaaccg ccagtgcact
     5821 ggctccgagg gcacctgccc gagcagcatc tcggtcgcag cggacgtgcc ggccgccggc
     5881 gcactgcccg gctggctggt ggcggccgac ctgccccgaa tcaccccggc cgccggccgc
     5941 tggggagcca ccgatccggc gaccacgctg cacctgatcg gcagccagtg cgaggtcatt
     6001 gacctgaacg cggtcaccgg caccaccagc gtcggccagc gcaccttcct cttggctgac
     6061 gacgcaaagg cgccgtccgg cttcggcatc gaccaggcgg tgtacagctt cagcgcggcc
     6121 aaggccgcca cgaacctgcg cgacaagctg gccaagaaca tcgatggatg ccccgaccgg
     6181 gcaccgaccg cgaaggtgac cgaaggtccg agcatcaagg gcaccggggt caacggcgcc
     6241 aagattgcgg gctcgaccta cctggtcacc cagagcactg gcacgaccac cgtggccttc
     6301 cgggtcgcgg tgctgaccgt ggacacccgc atggtttacc tgctcgccaa cccgtccagc
     6361 agcttcgact tcagtgacga cgactggcgc caactcgccg tccgcgccgg ccagcgggtc
     6421 agtcagagca gctgagctgg cagcgtggtc ggacccgcgc cacaaaagag gagcagcacc
     6481 tgccgctacc tggcctgact caatcgaggt agcccgtctc agggttgata ttgtccatga
     6541 actcgcggat gacatcttcc agtatctgcg gtgtcatctc tgcttcccgt ccgcccaagt
     6601 tcatcgaaaa gccatagttg gggctgttct ccctagtcca gctgagatca tattgaccgt
     6661 ctccacggtg gctcacgtcc cacagctcgc cgtcgacgct catacggcat accccctctg
     6721 gttcagtcat cgacagctgc ccgctcgctc gaaagatggt ctgaggcaac gcactcaacc
     6781 agttgctcaa tagcaggaga gagtccgatc cactgtgtac gcaagcgggg cggagtctgc
     6841 gtgcccaacg atgtccgcaa ggattatgga ccgccattgg tgactgctgg agttcacaca
     6901 cttcaccttg ttgttgactc gctgagtgct cccgctccct ggtgcccggt tctttccggt
     6961 taccgcgctg ccctgaaagt accaagtgcc gttgagcttc tcctccagcc agatcgtcac
     7021 gtcggcgcgg tagccacttg ggcaggtgag attccaccag tacnnnnnnn nnnnnnnnnn
     7081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7141 nnnnnnnnnn nnnnnnnnnn nnnacacttc cctctgggtg ggttcgagtg caccgtcgca
     7201 ctcctatgtc cagtatcgaa gacgcaggca tgccgccgtc aaccagaaag tgtacgacct
     7261 tcactattgc tcctacgtat acgcgacagt tcgtgagcgg ggcacggctg cgatggctca
     7321 gctgggcccc cctcgaactc cagatgcgga cggtcgacct ctcggccaag gagggtccgc
     7381 gggcgcggta agcacgggca cgggcatggg cccttccgag agcagtcatg accgtgggac
     7441 cacccgtgtg gtctacttgc tggccagccc gtccagcagc ttcgacttca gcgacgacga
     7501 cgcctggcgc gcgaactggc cgtccgtgcc ggccagcggg tcagtagagc agctgatcgg
     7561 ccgcagatca gtggagtaat ccactaggca gaggtggagt aatccatcaa gagctggtgg
     7621 agttatcccc tatccttgcc tatgctggcg tctgtgactt ggtatgttcc ccggatcatc
     7681 gacgcccagg ccgagcgtgc cgtcgccagt gctggcgcag tcgtatttcg cggtccgcgc
     7741 gcggtgggaa agaccgaatc ggctctccgc ctggcggctt ccgagttgcg ccttgactcc
     7801 gccgatcctc gggccgccct ggcgaggacg cagcccggct ccgccctgga cggcgcgacg
     7861 ccccggctgc ttgacgagtg gcaggtagtc cccgaactgt ggaacgaagt gcgccacgcg
     7921 gtcgacgatc gccgagccag cggtcagttc atcatgaccg ggtcggcctg gcccgacgat
     7981 gaccgactgc tgcactccgg tgccggacga ttccgccaag tgaccgtacg gacgatgtca
     8041 ctcgcggaga cgggcgaatc gacgagtgcg gtgtcgctcg ccgggctgtt tgaaggtgac
     8101 ctgagaatgg cagagtcagc gacggacttc cgtggagtgg ttcagcggct ggtgcgtgga
     8161 ggatggcctg ggtggctgga cgccgatgag cccgcagcta tcgccagggc aacctcctac
     8221 gtgaccgaca tcgccgaaca cgacttcgtc acagtcgccg gcccgcgccg cgatcctcgc
     8281 cggttagtcg ccttcctacg cgcgctggcg gggctgactg ctcaaccagc aagctacgct
     8341 gcgatcaccc ggcgcatgaa cgagaagttc acgaacatag gcgctgccgc ggttcccgag
     8401 ctccacgacc tcgcccagag gctttatctc gtcgaggacc agcccgcctg gtcgccgtcc
     8461 cttcgatcac ggacggcgtt actgcaaact ccgtccaggc atctggccga cccttcgctg
     8521 gccgcagcac tgctcagcgc gggaaccgat cgcctactga gcgagccccc taccctcggc
     8581 ttcatcttcg agtctcaggt ggtccacgac ctgcgggtct atgcccaagc ggcagactcg
     8641 cgcggcgtct tccactatcg cgatgcaaaa gcacgcgatg agatcgacgc ggtagtcgag
     8701 gcgtccgacg gtcgctggct ggcggtcgag gtcaagctcg gcccggcaca ggtggacgcg
     8761 gcagctgcga atctgcttcg agtaaccgcc aaagtctccc ggcccccatc ggcgtgtgtc
     8821 gtcgtcaccc cgagcggagt ggcgcaccgg cggcccgacg gcgtcatggt cgtgccgctg
     8881 actgtgctgg gcccctgacc gacccgtggc cgctcggacg aacccgcaac tcgttccgag
     8941 cgaactcaac ccaggccggc gacgatctcg cgtagcaggg ccgcggtctc gctgggcgtc
     9001 cggcccaccc ggacgccgga tgcctccagc gcggccttct tggcccgcgc cgtgcctgcg
     9061 gagccggtga cgatcgcacc agcgtggccc atggtcttcc caaccggggc ggtgaagccg
     9121 gcgacatagc cgaccacggg cttggtgacg tgctcggcga tgtaggcggc ggcccgctcc
     9181 tccgcgtcgc cgccgatctc gccgatcagc acgatcgcct tggtgtcggg gtcgtcctcg
     9241 aaggcggcca ggacgtcgat gtgggtgctg ccgttcaccg gatcaccgcc gatgccgacc
     9301 gcggtcgaga agccgatgtc ccgaagctcg aacatcatct ggtaggtcag cgtgcccgac
     9361 ttcgacacca ggccgatcgg gcctgcgccg gcgatgttcg gcggaatgat gcccacgttc
     9421 gacttccctg ggctgatcag gccgggacag ttcgggccga tcagccgggt gtgtccctgc
     9481 tgccgggcgt aggcgacgaa ctcggcggtg tccttcaccg ggatgccttc ggtgatcacc
     9541 acggtgaggt cgaggttcgc gtccaccgca tcgatcaccg ccgcccgggc gaactttgcc
     9601 ggcacgaaga ccacggacgt gttcgcgccg gtggcggcct tcgcgtccgc caccgtgtcg
     9661 aagactggta ccgggtcctc ctcgaagacc accgactgcc cacccttgcc cggcgtgacg
     9721 ccgccgacga tgcgggtgcc cgcaccgaac atccgctggg tgtgcttacg accctccgcg
     9781 ccggtcatgc cctgcacgat gaccttggag cgctggtcca accagatcga catgctcagc
     9841 tctccttatt cggctgcggc gagttcggcg gcccgccggg ccgcttcgtc catggtggac
     9901 accagttcga ccagcgggtg cgccgcttcg gccagcatgt gccggccgat ctcgaccgcg
     9961 ttcccgtcca ggcgcacgac gatcggacga gtggcccggt cgccgaggat ggcgagcgcg
    10021 tcgatgatgc ccttggccac ttcggagcag gccgtgatgc caccgaacac gttgacgaac
    10081 accaccttca cctgcgggtc ggaaaggatg atgtcaagac cgttggccat cacctgcgcg
    10141 gacgcgccgc cgccgatgtc caggaagttg gccggacgcg gcgtgccggg caggtcggca
    10201 ccagccgcgg cgaccacgtc gagggtgctc atcaccagcc cggcgccgtt gccgatcacg
    10261 ccgaccgacc cgtccagctt cacgtaattg aggccctttg cccgcgcctt cacctccagc
    10321 gggtcgtcgg aggactcgtc ggcgtagtcg gcccactcgg gatgccggaa agctgagttc
    10381 gcgtcgaggg agaccttgcc atcgagcgcg acgatctgcc cagacccggt cttcaccagg
    10441 gggttcacct cgaccag
//
LOCUS       DAOPRQ010000080        10402 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_5396_length_10402_cov_14.538417, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000080 DAOPRQ010000000
VERSION     DAOPRQ010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 10402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10402
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5396_length_10402_cov_14.538417"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(132..1586)
                     /locus_tag="PKJ61_09990"
     CDS             complement(132..1586)
                     /locus_tag="PKJ61_09990"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOC14191.1"
                     /translation="MAEVVILGAGVSGHTAALHLRRMLGRNHTITVVSPNSQWNWIPS
                     NIWVGVGEMSKKDVVFPLAPVYQRKGIQFRQAKAVAIRPAGDTHDGRGAVDIEYTSKE
                     KSGQADQLRYDYLINATGPKLRFEATEGMGPDGHTVSVCTADHAMEAAEKLREVIAVL
                     KSGKPQTLVVGMGHGTCTCEGAAFEYVFNVDHELREAGVRDLARVVYLTNEAALGDFG
                     VDGMVFDQQGFQTTSELWTGSLFRERGVEVILGAHVQKVEAGLIHYETLDGATYTLDF
                     DFAMLIPPFGGVPLKAYAPDGSDITSELFAPSGFMKVDADYTAKPYAEWRAEDWPTTY
                     AAPGYDNLWAVGIAFAPPHQISKPLKTPNGTMIAPSPPRTGMPSGVMGKTVAKTIADR
                     IRHGADAPAHTASMARMGAACVASAGTGLTKGSAAAMTMLPVVPDYQRFATGRDEKET
                     RGELGLHGHWVKLMLHYLFLYKAKALPGWQMIPE"
     gene            1846..2349
                     /locus_tag="PKJ61_09995"
     CDS             1846..2349
                     /locus_tag="PKJ61_09995"
                     /inference="COORDINATES: protein motif:HMM:NF016221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HOC14192.1"
                     /translation="MRRVLMMLVAVLVAPLAACASSATPSAAAALMVESPWVRTTTGA
                     KDPSMTAAFLTVVNPGAADVRLVSADCADAGMVQIHEMVKSGDKMMMQEAKDGALVPA
                     GGHLHLTPGGYHVMLMRLSREFAVGDSVNLTLHFSDGSSLPVTAPVKDFVEEEDHYHS
                     PSPTPSS"
     gene            2349..3563
                     /locus_tag="PKJ61_10000"
     CDS             2349..3563
                     /locus_tag="PKJ61_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012921321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HOC14193.1"
                     /translation="MTEQSSEPSGVDRRHLFGYAGAAGVGALAGFGGGRASAPAPSVP
                     VAVTRQTYSPHDVHQAGIVTRTPAFTQLVSFRLLQPSVDSLQKLLKLWSGDIPPLMAG
                     KPVPADPTPELAQAGVSLSVTVGLGPRVFSLPGLERKRPDGFVEIPAMQHDRLQERWN
                     GGDVLAIVAADDATTVDYAARRLTRDAASFAEVAWVQSGSWRGVDASGAPVTGRNLFG
                     QVDGSGNPAGELLDATVWATDPLPWFVGGTTLVVRRIEMDLDFWDRTTRERQEKVIGR
                     RLDNGAPLTGQHETDALDLVATDADGRPVIPVDAHARRSHPDENDNRRIARRGVNYTH
                     TEVVDGRPVTSAGLVFQSFQANIAQQFVPIQNRLDSGDALNDWTHAIGSAVFAIPGGF
                     PEGDWIARALFE"
     gene            complement(3604..4110)
                     /locus_tag="PKJ61_10005"
     CDS             complement(3604..4110)
                     /locus_tag="PKJ61_10005"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOC14194.1"
                     /translation="MATRGEPKAHRLYLLRHAEALSVGPDWRDASRELSARGRTQARA
                     VGEKLASQPVELVLCSTAIRARQTAELLKIPAPIRHLDRLYNCGVRQLLAEVAGVSDL
                     VRTVLIVAHAPGIPGLAAGLADPETSDPAALDRIQYNYPPATLVGLEFQGSWSELVDA
                     RLFLAHTP"
     gene            complement(4128..4685)
                     /locus_tag="PKJ61_10010"
     CDS             complement(4128..4685)
                     /locus_tag="PKJ61_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016456732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HOC14195.1"
                     /translation="MTVDRPSFARPKIQPSLHFDVTVEIPKGTKNKYEMDHHTGRIRL
                     DRTLFTSTQYPNDYGFIEGTLGQDGDPLDAMVLLPEATFPGALIECRAIGMFRMKDEM
                     GGDDKVLCIPVADQRQSSIKELTDLPSLTLLEIEHFFSVYKDLEPGKSVEGAVWVGHA
                     EAEVEIRASWARARGTSYENEFTRE"
     gene            4715..6106
                     /gene="dacB"
                     /locus_tag="PKJ61_10015"
     CDS             4715..6106
                     /gene="dacB"
                     /locus_tag="PKJ61_10015"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HOC14196.1"
                     /translation="MCEPSLSLAAARARWRSSAGGSAGPLRIVAACLAALLFAGCSTQ
                     AFNPNEVLPSAVVPVPSADASPTTPVAPQPTLIPIPEKLSVQLKKVSRSGIGAGGVAV
                     LADNGSMLTSRSPDKAVAPASTMKVLTSLAAVDLLGADHTFTTKVVDAGSGRIVLVGG
                     GDPLLTIKPSKSVNKPASLTALAQATAVALTASGVAKVSLGYDASLFSGAGYSPKWKS
                     TWRSYTARVSSLVVGSGMANKWQAAPDPALATAKAFAAKLKAAGIAVKLAGAAKAPAD
                     ASELASVQSAPLSRIVARTLQLSDNLAADMLARHVALASGQPATFAGGATATRQWLSG
                     HGLWADGMRLYDGSGLARDARVTPSVLAHAVQLSLNTERLAAVAQGLPVAGQNGTLKD
                     RFNDKSEKAGRGNVHAKTGTLRGIAALAGYLTTADGARLAFALMATNTVGQTTAYNWL
                     DRTAAAMVRCGCS"
     gene            complement(6114..7724)
                     /locus_tag="PKJ61_10020"
     CDS             complement(6114..7724)
                     /locus_tag="PKJ61_10020"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HOC14197.1"
                     /translation="MHPQLQRLAAVLLTVLLGLGLVPSTTDAAASAAPKVTKLSVKTG
                     PTAGGTSVVIRGKNFTADAVVTFGSTPATSVRFVSSKKLVATTPASAAGRTDVRVQTA
                     SGRSKTGKRSRFTFIAPPAIASLSTTAGPVKGGKRITIKGSGFTKVSKVLFGGVKGKK
                     LKVKSSKKLTVVVPARPNGYAEVKVVGRYGTSAGARYLYGTAPAAPPLVTGGTQQLAV
                     ASFNVTVATATTTPTATMRPWAERRSAVAGQLLREGIDVVGVQEASASARQVPTGVAQ
                     FQDLTNLMNELGGTYALTNTAEYCDGPAYAKCPNGAGQSDRIIFNTSRLALDRQGSRS
                     LDDRTDRSGSARHVTWAQLTDRVTSRSFFFVSTHLEPLDAKDDDAEGIRRSEALRAGQ
                     ASLILTEIAEENTARLPVVMVGDLATTKFTDLNDNEATNSAHQAFIDAGYADPLGNTY
                     RLKSRNDSVFAQSLVNAQYSSLNDYLPAPKTLSGYEIGSYMDYILLSSRSITALEWKT
                     VVDLDAAGNFSGVIPSDHNLVKLTVLLP"
     gene            7824..8798
                     /locus_tag="PKJ61_10025"
     CDS             7824..8798
                     /locus_tag="PKJ61_10025"
                     /inference="COORDINATES: protein motif:HMM:TIGR03883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HOC14198.1"
                     /translation="MEHIPEVDWALAARTGRALVHPGPELSRTEVAAVVAELRQAAAT
                     ATGHIAEVTQLDRPAPAEVLVVDRASWLAAVTQSAQAMLAGVGAETAAEKRSLPAQVS
                     SKALGVQAAGVFALVANRILGQFDPFVSPSRLLLVAPNIVSVERQLAAVPSDFRLWVC
                     LHEETHRFQFGHASWLKGHLLGLLGELVDGDELRFGWPDGKDGIRGLIGTPEQKQAFE
                     QVTALMSLMEGHADVMMDRVGAEVVPSYESIRKSFEARRNAGGWGAWLRKLLGLDLKR
                     AQYRDGAKFCREVIASADVATLNRAFEAPGLLPSLAEIHDPQRWLHRL"
     gene            8798..9775
                     /gene="tilS"
                     /locus_tag="PKJ61_10030"
     CDS             8798..9775
                     /gene="tilS"
                     /locus_tag="PKJ61_10030"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012924019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOC14199.1"
                     /translation="MAKRALGPATQQVVAAVEAVADAPILVACSGGPDSLALAAAAAI
                     VGRRRGLPVRAVTVDHGLQPGSAAVAELVVARLAALGLAATVLAVDVVAAGLGMEAAA
                     REARYAALACEAEPDEDVLLGHTLDDQAETVLLGLARGSGARTLAGMASRRGRFVRPL
                     LGVRSVTTRAACAELGLEPWHDPHNSDERFARVRARRSVMAVLESELGPGIAEALART
                     AELARADADLLDELAARELASQSDGDGLDCAWLESLPDAIANRVLLAWLRGSGGNDLS
                     AAHLASVRGLVHSWRGQRWVEVPGLRVARTGGRLVVRDPLAGVAAEPLG"
     gene            9785..10336
                     /gene="hpt"
                     /locus_tag="PKJ61_10035"
     CDS             9785..10336
                     /gene="hpt"
                     /locus_tag="PKJ61_10035"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HOC14200.1"
                     /translation="MDAADISTDLAEILYSKADIANRLAEVAAEIDRDYAGLNPLLVG
                     VLNGAVMVMADLSRALHIDCAMDWMAVSSYGMGTQSSGVVRILKDLSTDLVGRHVLVV
                     EDIIDTGLTLTYLMQNLASRGPASLEIMAMFRKPDAMKIEVDVKYIGFDLPNQFVVGY
                     GLDYAGRYRNLNDVGILAPHVYS"
BASE COUNT         1548 a         3430 c         3631 g         1793 t
ORIGIN      
        1 gtcaaaggcg atgaagcgca ggaactgacc gagcaggttc tgccggccgc gcagcgtctt
       61 cgcggtcggc agcggcgact tggacagatc ggcgaccggc tcgggagcgg gcaggttcga
      121 ctcagtcatg atcactccgg aatcatttgc cagcccggca gggccttggc cttgtagagg
      181 aacaggtagt ggagcatcag cttcacccag tggccgtgca ggccgagttc gccgcgggtc
      241 tccttctcgt cccggccggt ggcgaagcgt tggtagtcag gcaccactgg cagcatcgtc
      301 atcgcggcag ccgagccctt ggtcaacccc gtccctgcgg acgcgacgca ggctgcgccc
      361 atccgcgcca tcgacgcggt gtgcgccggg gcgtccgcgc cgtgccggat gcggtcggcg
      421 atcgtcttgg cgacggtctt gcccattacg ccggatggca tgccggtgcg tggcggcgag
      481 ggcgcgatca tcgtcccgtt cggggtcttc agcggcttcg agatctggtg cggcggtgcg
      541 aacgcaatcc cgaccgccca caggttgtcg tacccgggag cggcgtaggt ggtcggccag
      601 tcctcggcgc gccattccgc atagggcttg gcggtgtagt cggcgtccac cttcatgaag
      661 ccgctgggtg cgaagagttc gctggtgatg tcgctgccat cgggtgcgta ggccttcagt
      721 ggcaccccgc cgaacggcgg gatgagcatc gcgaagtcga agtcgagagt gtacgtggcg
      781 ccgtccaggg tctcgtagtg gatcagcccg gcctccacct tctgcacgtg cgcaccgagg
      841 atgacctcga cgccgcgttc gcggaacaga gaacccgtcc acagttcgct ggtggtctgg
      901 aaaccctgct ggtcgaagac catcccgtcg acgccgaagt cgcccagcgc tgcctcgttg
      961 gtcaggtaga ccacgcgggc caggtcgcgg acgccggctt cgcgcaactc gtggtcgacg
     1021 ttgaagacgt actcgaacgc cgctccctcg caggtgcagg tgccgtgacc catgcccacc
     1081 acgagggtct gcggcttgcc gctcttgagc accgcgatca cttcgcgcag cttctcggcc
     1141 gcctccatgg cgtggtcggc agtgcagacc gacaccgtgt gtccgtccgg tcccatgccc
     1201 tcggtggctt cgaagcggag cttcggaccg gtggcgttga tcaggtagtc gtagcgcaac
     1261 tggtcggcct gaccggactt ttccttcgac gtgtactcga tgtccaccgc gccacggccg
     1321 tcgtgcgtgt cgccggcagg gcggatcgct actgccttcg cctggcggaa ctggatgccc
     1381 tttcgctggt agaccggcgc gagcgggaag acgacgtcct tcttgctcat ctcgccgacg
     1441 cccacccaga tgttggacgg gatccagttc cactgcgagt tcggcgaaac cacggtgatg
     1501 gtgtggttgc gaccgagcat tctgcgtagg tgcagggctg cggtgtggcc ggacactccg
     1561 gctcctagga tgactacctc tgccattggt gctccttctg cggctgcgtt gccgacgtct
     1621 tcagcctata cgggttgggg tatcgacacg ggcttcttca gaagtttcag tggctgatgc
     1681 ggacgcctcg atctgaacgc cagccttagc gctcgagcgc cagcaccagt ggctagggct
     1741 cgatgcttgc ggctagcgct cggcgccgcc cggctaagtc ttccagttca atgactccgt
     1801 ggggtccggc ttgaggcatg cttggacgac ggaataggag ttcagatgcg tcgagtgctg
     1861 atgatgttgg tggcggtcct ggtggcgccc ctggccgcat gcgcgagcag cgcaacgccg
     1921 agcgctgcgg ccgccctgat ggtcgagagc ccatgggtgc gcaccaccac gggcgccaag
     1981 gatccgtcca tgaccgccgc gttcctgacg gttgtcaatc cgggcgcggc cgatgtgcgg
     2041 ttggtctcgg ctgactgcgc ggacgccggc atggtgcaaa ttcacgagat ggtgaagtcg
     2101 ggcgacaaga tgatgatgca ggaggccaag gacggcgcgt tggtgccggc cggcggtcat
     2161 ctgcacctga ccccaggcgg ctaccacgtc atgctgatgc gactgtcgcg cgagttcgcg
     2221 gtcggcgatt cggtgaacct caccctgcac ttctcggacg ggagttcgct gccggtcacg
     2281 gcaccggtga aggacttcgt cgaggaggag gatcattacc acagcccgtc cccgacccca
     2341 tcgagctgat gaccgagcaa tcctccgagc cttccggggt cgaccggcga cacctgttcg
     2401 gctacgccgg ggcggcaggt gttggtgcgc tcgcgggttt cggtggcgga cgtgcgtccg
     2461 cccctgcgcc gagcgtgccg gtggcggtga cccggcagac ctattcgccg cacgacgtcc
     2521 accaggccgg catcgtgacc cgcacgccgg cgttcaccca attggtgtcg ttccggctgc
     2581 tgcagccgtc ggtggactcg ctgcagaagc tgctcaaact gtggagcggc gacattcctc
     2641 cgctgatggc cggcaagccg gttccggccg atcccacgcc tgagctagcg caggcggggg
     2701 tgtcgctttc ggtgaccgtc gggctcggtc cgcgggtgtt ctccctcccc gggctggagc
     2761 gcaagcgccc ggacgggttc gtggagattc cggccatgca gcacgaccgg ctccaggagc
     2821 gctggaacgg cggcgatgtg ctggcgatcg tcgccgcgga cgacgccacg acggttgact
     2881 acgcggcacg gcggttgacc cgggacgcag cgagctttgc cgaagtggcg tgggttcagt
     2941 ccggctcctg gcggggagtg gacgcgtccg gcgcgccggt gaccggacgc aacctgttcg
     3001 gccaggtgga cggttcgggg aatccagctg gagagttgct ggacgcgacc gtctgggcca
     3061 ccgacccgct gccctggttc gtgggcggga cgacgctggt ggttcggcgg atcgagatgg
     3121 acctcgactt ctgggatcgc accactcgcg agcgtcagga gaaggtgatc ggccggcgcc
     3181 tggacaacgg cgccccgctg accgggcagc acgagaccga cgccctcgac ctggtggcga
     3241 cggacgccga tggccgtccg gtgatccccg tggacgcgca cgcgcgccgc tcccacccgg
     3301 acgagaacga caatcgccgg atcgccaggc gtggcgtcaa ctacacccac accgaggtgg
     3361 tcgacggccg cccggtgacc agcgccggac tggtcttcca atccttccag gcgaacatcg
     3421 cccagcagtt cgtcccgatc cagaaccgcc tggactcagg cgacgctctg aacgactgga
     3481 cccacgccat cggatccgcc gtattcgcga tcccaggcgg cttccccgaa ggtgactgga
     3541 tcgcccgcgc cctgttcgag taaccccaac ggtccggcct cggcacccga tcggcaagac
     3601 cgctcagggc gtgtgggcga ggaagaggcg ggcgtccacc agttcggacc agctgccctg
     3661 gaactccaga ccgaccagcg tggccggggg gtagttgtac tgaatccggt ccaaggcggc
     3721 tgggtcggag gtttccggat cagccaggcc cgccgccaga ccgggaatcc cgggtgcgtg
     3781 agccacgatc agcaccgtcc gcaccaggtc ggatacgccg gccacctcgg caagcaactg
     3841 ccggacgcca cagttgtaga gccgatcgag gtgccggatc ggcgccggta ttttcagcag
     3901 ttcggcggtc tgtcgcgccc ggatcgccgt cgagcagagc accaactcca ccggctggct
     3961 cgccagcttc tcaccgaccg ctcgcgcctg cgtccgtccg cgcgcagaca actcgcggct
     4021 ggcgtcgcgc caatccggac cgacgctcag ggcctcggca tggcgcaaca aatagagccg
     4081 gtgagccttc ggttcgccgc gagtcgccat tcagtcgcca gggaggatca ctcccgggtg
     4141 aactcgttct catacgaggt gccgcgagcg cgtgcccagc tggccctgat ctcgacctcg
     4201 gcctcggcgt ggcccaccca cacggcgcct tcgaccgact tccccggttc gaggtccttg
     4261 tagacgctga agaagtgctc gatctcgagc agcgtcaggc ttggcaggtc ggtgagttcc
     4321 ttgatgctgg actggcgctg gtcggccacc gggatgcaca gcaccttgtc gtcgccgccc
     4381 atctcgtcct tcatccggaa catcccgatt gcgcggcact cgatgagcgc gccggggaac
     4441 gtcgcctccg gcagcagcac catcgcgtcc agcggatcgc cgtcctggcc gagggtgccc
     4501 tcaatgaacc cgtagtcatt ggggtattgg gtggacgtga acagcgtccg gtcgagccgg
     4561 atccggccgg tgtggtgatc catctcgtac ttgttcttcg tgcccttggg gatctccaca
     4621 gtgacatcga aatgcaggct gggctggatc ttcggacggg cgaaagaagg gcgatccacg
     4681 gtcatctgag cctctctgtc atgattggta ctcgatgtgt gagccgagcc tatcgttagc
     4741 cgctgcccga gcaaggtggc gctcctcggc cggtgggtcc gccgggccgc tccggatcgt
     4801 cgccgcgtgt ctggccgcgc tgcttttcgc cggctgttcg acccaggcgt tcaaccccaa
     4861 cgaggtgctg ccgtccgcgg tcgtaccagt gccaagcgcg gacgcgtccc cgaccacacc
     4921 ggttgcgccc cagcccacgc tgatcccgat ccccgagaag ttgtcggtgc agctgaagaa
     4981 ggtttcgcgc agtggcatcg gggccggcgg cgtggccgtg ctcgctgaca acggctcgat
     5041 gctgaccagc cgcagccccg acaaggccgt ggcaccggcg tccacgatga aagttctgac
     5101 cagccttgct gccgtcgatc tgctcggcgc cgaccacacc ttcaccacca aggtcgtgga
     5161 cgcaggttca ggacggatcg ttctggtcgg cggcggcgac ccgctgctca cgatcaagcc
     5221 gtccaagtcg gtcaacaagc ccgcttcgct gaccgcactg gcccaggcga ccgctgtggc
     5281 gctgaccgcg tccggggtcg cgaaggtcag tctgggctac gacgcgtcgc tgttcagcgg
     5341 cgccgggtac agccccaagt ggaaatccac ctggcgcagc tacaccgctc gggtgagctc
     5401 gctggtggtc ggctcgggca tggcgaacaa gtggcaggct gcgcccgatc ctgcactggc
     5461 tacggcaaag gccttcgctg ccaagctcaa ggctgcgggt atcgcggtga aattggccgg
     5521 tgcggcgaag gcgccagcgg acgcgtccga gctggccagt gtgcagtcgg caccgctgag
     5581 ccggatcgtc gcgcgcaccc tgcagctcag cgacaacctc gccgcagaca tgctggcgcg
     5641 ccacgtcgcg ctcgcgtccg ggcagcctgc gaccttcgcc ggcggcgcca cggcgacccg
     5701 gcagtggctg agcggccacg gactctgggc cgacggcatg cggctatacg acggcagtgg
     5761 cctggcccgc gacgcccgcg tgaccccatc ggtgttggcg cacgccgttc agctctcgct
     5821 gaacacggag cggctcgcgg cggtggcgca ggggctgccg gtggccggcc agaacggcac
     5881 gctcaaggac cgcttcaacg acaagtcgga gaaggccggc cgcggcaacg tgcacgccaa
     5941 gaccgggacg cttcgtggaa tcgccgcgct ggccggctac ctgaccacag cggacggcgc
     6001 ccggctcgcg ttcgcgctga tggccaccaa caccgtcggc cagaccacgg cctacaactg
     6061 gctggaccgc acggccgcgg cgatggtgcg ttgcggctgc agctgacctt gccctagggc
     6121 aggaggacgg tcagcttcac caggttgtga tcggacggga tcacgccgga gaagttgccg
     6181 gcggcgtcca gatcgaccac cgtcttccac tccagcgcgg tgatgctgcg gctcgatagc
     6241 aggatgtagt ccatgtacga gccgatttcg taaccggaca gcgtcttcgg tgccggcagg
     6301 taatcgttga ggctgctgta ctgggcgttg accaggcttt gcgcgaagac cgagtcgttg
     6361 cggctcttga gtcgataggt gttgccgagc gggtcggcgt agccggcgtc gatgaacgct
     6421 tggtgggcgg aattcgtcgc ttcgttgtcg ttgaggtcag tgaacttggt ggtagccaga
     6481 tcgccgacca tgaccaccgg tagccgcgcg gtgttctcct cggcgatctc ggtcaggatc
     6541 aagcttgcct gcccggcgcg caacgcctcg gatcgccgga tgccttcggc gtcgtcgtcc
     6601 ttggcgtcga gcggctccag gtgggtgctg acgaagaaga agctgcggct ggtgacgcga
     6661 tcggtcagct gagcccaggt gacgtgccgg gccgaaccgc tccggtcggt ccggtcgtcc
     6721 agcgaacgcg agccttgccg atccagcgcc agccggctgg tgttgaagat gatccggtcg
     6781 ctctggccag cgccgttggg gcacttggcg taagccgggc cgtcgcagta ctccgcggta
     6841 ttggtcaggg cgtacgtccc gccgagctcg ttcatcaggt tggtcaggtc ctggaactga
     6901 gcgaccccag tgggcacctg cctggccgat gcggacgcct cctggacgcc aaccacatcg
     6961 atgccctccc gcaggagttg tcccgcgacc gcgctgcgcc gctcggccca cggacgcatg
     7021 gtggccgtcg gggttgtcgt ggcggtggcg acggtgacgt tgaagctggc caccgcgagc
     7081 tgctgggtgc cgccggtgac cagcggcggg gcggcgggcg cggtgccgta caggtagcgg
     7141 gcgccggctg aggtgccgta gcgtccgacg accttcacct ccgcgtaccc gttcggccgg
     7201 gcgggcacca ccacggtcag cttcttcgac gacttgacct tcagcttctt gcccttgacc
     7261 ccaccgaaga gcaccttgct gaccttcgtg aatcccgacc ccttgatcgt gatccgcttc
     7321 ccgcccttca ctgggccggc ggtcgtgctc agcgatgcga tcgccggagg tgcgatgaag
     7381 gtgaaccggg agcgcttgcc ggtcttggag cgtccggacg cggtctgcac ccgcacgtcc
     7441 gtccgtcccg cggcgctggc cggtgtggtt gcgaccagct tcttcgagga tacgaagcgc
     7501 actgaggtgg ccggcgtcga cccgaacgtc accacggcat cagcggtgaa gttcttcccg
     7561 cggatcacga cgctggtgcc gccggcagtg gggccggttt tcaccgacag cttggtcacc
     7621 ttcggggcgg cggacgccgc cgcgtccgtg gttgacggaa ccaggccgag gccgagcagg
     7681 acggtaagga gaacagcggc gagccgctgc agctgggggt gcatgcccgc attctggcag
     7741 aaggccgctg cggcggcagg ctatggaatt cattggctca gccgcagcgg ctcggaaaac
     7801 agaccacaag tagggttgac gccatggagc acatccccga ggtcgactgg gcgttggcgg
     7861 cccgcactgg acgagcgctg gtgcacccgg gtcctgaact cagccggacg gaggtcgcgg
     7921 ccgtggtcgc cgaactgcgg caggctgcag ccaccgcgac cgggcacatc gccgaggtca
     7981 cccagctcga ccggccggct ccggctgaag tcctggtggt cgatcgggcc agctggctcg
     8041 ctgcggtgac ccagtccgcc caggccatgc tggccggcgt gggtgccgaa accgctgctg
     8101 agaagcgctc cttgccggcg caggtgagct cgaaggcgct cggggtgcag gccgccggcg
     8161 tgttcgccct ggtcgccaac cgcatcctcg ggcagttcga cccgttcgtc agcccgtccc
     8221 ggttgctgct ggtcgccccc aacatcgtct ccgtcgagcg tcagctcgcc gcggtgccga
     8281 gcgacttccg gctgtgggtg tgcctgcacg aagagacgca ccgcttccag ttcgggcacg
     8341 cgtcgtggct caagggtcac ctgctcgggt tgctcggcga actcgtggac ggcgacgaac
     8401 tgcgcttcgg ctggcctgac ggcaaggacg ggatccgcgg cctgatcgga acgcccgaac
     8461 agaagcaggc gttcgagcag gtcaccgcgt tgatgtcgtt gatggagggc cacgctgacg
     8521 tgatgatgga ccgtgtcggc gcggaagtgg tgccgagcta cgagtcgatc cgcaagtcgt
     8581 tcgaggcgcg caggaacgcg ggcggctggg gagcctggct gcgcaaactg ctcggcctgg
     8641 acctcaaacg cgcgcaatac cgggacggag cgaagttctg ccgcgaggtg atcgcgtccg
     8701 ctgacgtggc gacgttgaac cgcgccttcg aggcgcctgg actgttgcca tcgttggcgg
     8761 agatccacga tccgcagcgg tggctgcatc ggctctgatg gcaaagcgcg ctctcggccc
     8821 ggccacccag caggtcgttg ctgcggttga ggcggtagcc gacgcaccga tcttggtggc
     8881 gtgctcgggc gggccggatt cactggcgct ggcggcggcc gccgcaatcg tcggacggcg
     8941 gcgcgggctg ccggtgcgcg cggtgaccgt cgaccatggg ctgcagcccg gttcggcagc
     9001 ggtggctgag ttggtggttg cccggctcgc tgccctcggg ttggcggcca cggtgctggc
     9061 ggtggatgtt gtcgctgccg ggctgggcat ggaagcggca gctcgagaag cccgctacgc
     9121 ggccctcgct tgcgaggccg agcccgacga ggacgtgctc ctgggccaca cgcttgacga
     9181 ccaggccgaa accgtcctgc tcggcctggc tcgcggctcc ggcgcgcgaa cgctggccgg
     9241 catggcgtca cgtcgcggac gcttcgtccg gccgctgctg ggggtgcgtt ccgtcacgac
     9301 gcgcgcagcc tgcgccgagc tcgggctgga accctggcat gacccgcaca actcggacga
     9361 gcgcttcgcc cgcgtccgcg cccgccgctc agtgatggcg gttctcgaat ccgagctggg
     9421 gcctggcatc gccgaagcgc tggcccgcac cgctgaactg gccagggcgg acgccgatct
     9481 gctcgacgag ctggccgcgc gcgaacttgc gtcccaatcc gacggtgatg ggctggattg
     9541 tgcctggctg gagtccctgc cggacgcgat cgcgaatcgc gtcctgctgg cctggctgcg
     9601 aggttccgga ggcaatgact tgtcggctgc gcacctcgcg tccgtgcgcg ggctggtgca
     9661 ctcctggcgt ggccagcgat gggtggaggt gcccggcctg cgagtggcgc ggacgggtgg
     9721 tcggctggtt gtccgcgatc cgttggctgg ggtcgccgcg gagccgttgg ggtaggtttc
     9781 atccgtggac gcagcagaca tcagtaccga tctcgccgag atcctctatt cgaaggctga
     9841 catcgcgaac cggctggctg aggtggctgc cgagatcgat cgcgactacg ccgggctgaa
     9901 cccgctgctg gtcggcgtgc tcaacggcgc ggtgatggtg atggccgacc tgtcccgtgc
     9961 gctgcacatc gactgcgcga tggactggat ggcggtctcg tcctacggca tgggcaccca
    10021 gtcgtccggg gtggtgcgga tcctgaagga cctctctacc gacctggtcg gacgccacgt
    10081 gctggtcgtc gaggacatca tcgacaccgg gctcacgctc acctacctga tgcagaacct
    10141 tgcctcgcgc ggtccggcca gcttggagat catggcgatg ttccgcaagc cggacgccat
    10201 gaagatcgag gtggacgtca agtacatcgg cttcgacctg cccaaccagt tcgtggtcgg
    10261 gtacggcctt gactacgccg gccgctatcg caacctgaac gacgtgggca tcctggcccc
    10321 gcacgtctac agctgacccc acctctccgc aaactggaca gtcctggccc gtcctgggcc
    10381 gcatccgtcc agtttctgct ga
//
LOCUS       DAOPRQ010000081        10155 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_5550_length_10155_cov_9.601782, whole genome shotgun sequence.
ACCESSION   DAOPRQ010000081 DAOPRQ010000000
VERSION     DAOPRQ010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 10155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10155
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5550_length_10155_cov_9.601782"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            126..1790
                     /locus_tag="PKJ61_10040"
     CDS             126..1790
                     /locus_tag="PKJ61_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14201.1"
                     /translation="MANTIVRGDCFRCGRVKNLRWNHALDRGECRACRARRSPQEACT
                     GCGKQRRVNARTSDGGALCVTCYARTRTADDACAECGSIGPLATRVGGKFEGSRNLCS
                     RCYRNPRRPCGICGRLKRVALKATATTPDVCPTCYQAPVIDCSICGQQALGRRTTNHG
                     RPRCFACQATQQIDAALSGPDGTINPELKPVRDALNEFRQPRSLLNNWHDLPSLHLLA
                     EIAQGRLDLSHDALDARPQTFSVTYLRSMLVATGALPPRDENAARLHRYAAEALANIA
                     DPEQRGVLIRYARWHVVGRAKTNRHGYISAAVTGRCKGDIQAAKGFFDHLTAHGQTLS
                     GCSQGYLDAWLATDRDRRLGFLRWLTRNGYLAGCRLPEPVRQKDPAHDADPSAQTDAA
                     RTLLHDPDSASIEDRAAACLILLYAQPAAKIVTLTTDDIEVRDGDTYLALGPESLLLV
                     PPLDALITALPVTKLFGTASTLADVRWLFTGKSAGSHLHPASLMRRMHRLGIIARTGR
                     NTALLHLASTTPPAVFASLIGIHVNTATRWAEMSGSAWNAYAAIRR"
     gene            complement(1985..3955)
                     /locus_tag="PKJ61_10045"
     CDS             complement(1985..3955)
                     /locus_tag="PKJ61_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HOC14202.1"
                     /translation="MSTACGCDEPATKPAGEAEEVERPWWRDPALLVPIFSGVAFVTG
                     LVLEWSGLETAALVVFWVGLLLGAYTFVPGAIRNLVTRRKLGIGLLMTISAVGAVILG
                     YVEEAAALAFLYSIAEALEDKAMDRARGGLRALLNLVPETATITRDGGQVEIPAKEIV
                     VGQVMVIRPGERLATDGIVRSGRSSLDTSAITGESIPVEVEPGDEVSAGAINTAGVLE
                     VETTAAGTDNSLTTIVELVEQAQAEKGDRARMADRIARPLVPGVMVLAVLVGVLGSLF
                     GDPGLWITRALVVLVAASPCALAIAVPVTVVSAIGAASKFGVITKSGAAFERLGGIRH
                     LAVDKTGTLTRNQPTVTAVIPADGTDEDKVLAAAAAIEQHSTHPLAAAIAAAAGAVPT
                     ARDVSEEAGHGITGIVEGHRVRVGSPRWIDPGPLASRVEDLEAAGQTCVLVTIDDEVA
                     GAIGVRDELRSEVPEVIETLRGQGVEVSMLTGDNTRTATALAQIAGIVDVHAELRPED
                     KARIVTEMAERSPTAMIGDGINDAPALAGASVGIAMGATGSDAAIQSADVAFTGHDLR
                     LIPQALLHARRGRAIINQNIVLSLAIIVVLLPLAITGALGLAAVVLVHEVAEVIVIAN
                     GLRAARTPRKDAVPAAGVASPSGRLAVAGGER"
     gene            complement(3952..4323)
                     /locus_tag="PKJ61_10050"
     CDS             complement(3952..4323)
                     /locus_tag="PKJ61_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009647418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOC14203.1"
                     /translation="MLTIASRVDVMNRLGRAMADPTRSRILLSLLEGPGYPAKLARKL
                     ELTRTNVSNHLTCLRGCGIVVSEPEGRQTRYEIADPHLAAALTALVDVTLAVDENVPC
                     IDPACTVPGCCDAQGSAEDAS"
     gene            complement(4540..4938)
                     /locus_tag="PKJ61_10055"
     CDS             complement(4540..4938)
                     /locus_tag="PKJ61_10055"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-responsive transcriptional
                     regulator"
                     /protein_id="HOC14204.1"
                     /translation="MRVAELASVVGVRPDTIRYYERAGLLPPPERTAAGYRQYDAGAV
                     DRLRFIQGAQRLGLRLEDIRDLLAVRDTGTCPCEPAGELLARRLTELDEEMVRLAELR
                     SQMVAMLGELPADDCPQPIPGTWLRKEGGD"
     gene            5510..5950
                     /locus_tag="PKJ61_10060"
     CDS             5510..5950
                     /locus_tag="PKJ61_10060"
                     /inference="COORDINATES: protein motif:HMM:NF014584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HOC14205.1"
                     /translation="MTDELSANEPLDTDAVADTGPGLRGIGLVALGGTAGTLARHALD
                     ALIGETAGLPVGIFIINITGAFALGVLIESLALGGPDRGRRRDLRLLLGAGLLGGYTT
                     FSTASLESIRLLTAKRYTAALLTGPGMLIACTTLSVAGILIIAP"
     gene            complement(6249..6629)
                     /locus_tag="PKJ61_10065"
     CDS             complement(6249..6629)
                     /locus_tag="PKJ61_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOC14206.1"
                     /translation="MTTSLPLLATDASGCCAPVTAGVMEVEDAQRLARVFKALGDPTR
                     VRLLSMIAAQSGGEACVCDLTEPVGLSQPTVSHHMKQLVDAGLVTREQRGKWAYYTII
                     PETLAMLSGALDPQALRAGAASSC"
     gene            6718..7593
                     /gene="arsM"
                     /locus_tag="PKJ61_10070"
     CDS             6718..7593
                     /gene="arsM"
                     /locus_tag="PKJ61_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HOC14207.1"
                     /translation="MGTNNDTAEKEELREQVRARYAGAATAVKAGARNENLLVEDACC
                     GTSCCGGAPADRDTTQPAAEDACCDSSCCGDTTATERSFGAGLYDAATTAGLPIEAVE
                     ASLGCGNPTAVTELHEGERVLDLGSGGGIDVLLSASRVGPTGFAYGVDMTDEMLDLAR
                     ENAAKAEATNVEFLKGTIEDVPLPNASVDVVISNCVINLSTDKPAVLAEMFRVLTPGG
                     RIGISDVVAEDHLSHEQRAERGSYVGCIAGALSKSEYLDGLAAVGFTDANVEFTHEAA
                     DGMHSAIIRATKPAA"
     gene            7608..8702
                     /gene="arsB"
                     /locus_tag="PKJ61_10075"
     CDS             7608..8702
                     /gene="arsB"
                     /locus_tag="PKJ61_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007078857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HOC14208.1"
                     /translation="MSTATAPVVAGARRLSRLDAWLPAWIGLAMIAGLLLGRFVPALS
                     DLLTRMEVAGISVPIGLGLLVMMYPVLAKVRYDKVAAVTGDKKLLVSSLVLNWLVGPA
                     VMFTLAWLFLPDLPEYRTGLIIVGLARCIAMVVIWNDLACGDREATAVLVAINSIFQV
                     IAFSFLGWFYLTLLPGWLGLDTQGLDVSVGKIALNVLVFLGIPLIAGFASRWIGEHRR
                     GRAWYEDTFLPRIGPWALYGLLFTIVLLFALQGDQIISQPLDVARIALPLLVYFALMW
                     FAGLGLGKSLGLGYARSTTLAFTAAGNNFELAIAVAIGTFGATSGQALAGVVGPLIEV
                     PVLVALVYVSLWTARRWFHTDPYQPETSPR"
     gene            8699..9100
                     /locus_tag="PKJ61_10080"
     CDS             8699..9100
                     /locus_tag="PKJ61_10080"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HOC14209.1"
                     /translation="MTTTKPTVLFVCVHNAGRSQMAAGLLTHLAGDRIEVRSAGSEPA
                     DQLNPAVIAAMREVGIDITAERPKLLTTDAVRTADVVITMGCGDTCPIFPGKRYEDWQ
                     LDDPAGQGIEAVRPIRDDIEARVRDLITQLT"
     gene            complement(9245..9655)
                     /locus_tag="PKJ61_10085"
     CDS             complement(9245..9655)
                     /locus_tag="PKJ61_10085"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HOC14210.1"
                     /translation="MSVPPDSVDDNDLRDGTRRMAIEPAAEPNQLSALLVERINSRDL
                     AGLVALYEDDAILDTSNGPVVGSAAIREFWSEFLATAPQVSLGQQAEPLVNGDLAFTS
                     TRVPGAFVTAEVARRQPDGSWKWAIDQPSLRVTD"
     gene            9771..10037
                     /locus_tag="PKJ61_10090"
     CDS             9771..10037
                     /locus_tag="PKJ61_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013601305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6112 family protein"
                     /protein_id="HOC14211.1"
                     /translation="MDVFPDFDGLGGIGDLRAVVGALLTFVLIVAVLMLIVSAIVWAI
                     ATAHGNYATASKGRIGVLVAVGAAVLAGGGVAWMNWLLTVGSSL"
BASE COUNT         1718 a         3825 c         3050 g         1562 t
ORIGIN      
        1 ccctacgtcg ccaccaactc gcggcgcgga cgcaagaccg gcaccaccgg caccacgccg
       61 ccgaagccaa gcaagaaccg tccgacccgt gctgtgatca agcctgccgg ggactgatgg
      121 gctgaatggc gaacacgatc gtccgcggcg actgcttccg ctgcggacgg gtgaagaacc
      181 tgcgctggaa ccacgccctg gaccggggcg aatgccgggc ctgccgcgcc cgccgctcac
      241 ctcaggaggc ctgcaccggc tgcggcaagc agcgccgggt caacgcccgc accagcgacg
      301 gcggcgcgct ctgcgtgacc tgctacgccc gaacccgcac cgccgacgat gcctgcgccg
      361 agtgcggcag catcggcccg ctggccaccc gagtcggtgg caagttcgag ggctcgcgga
      421 atctctgctc acgttgctac cgcaacccca ggcgcccctg cggcatctgc ggccgcctca
      481 aacgggtcgc actgaaagcc accgccacga cgccggatgt ctgcccgacc tgctaccagg
      541 cgccggtcat cgactgctcc atctgcggcc agcaggccct cggccgccgc accaccaacc
      601 acggccggcc ccgctgcttt gcctgccaag ccacccaaca gatcgatgcc gcgctcagcg
      661 gccccgacgg gacgatcaac cccgagctca aacccgtccg cgacgccctg aacgaatttc
      721 gccaaccccg atcactgctg aacaactggc acgatctgcc cagcctgcat ctgctggccg
      781 agatcgccca aggtcggctc gacctgtcgc acgatgccct cgacgcccgg ccgcagacgt
      841 tctcggtcac ctacctacgc tccatgctcg tcgccaccgg ggcgctgcca ccacgcgacg
      901 agaacgccgc ccgcctgcac cgatacgccg ccgaagccct cgccaacatc gccgacccgg
      961 aacagcgcgg ggtgctcatt cgctacgccc gctggcacgt cgtcggccgt gccaagacca
     1021 accgccacgg ctacatcagc gccgccgtca ccggacgatg caaaggcgac attcaggctg
     1081 ccaagggctt cttcgaccac ctcaccgcgc atggtcagac cctgagcggc tgctcgcagg
     1141 gctatctcga cgcctggctc gctaccgacc gcgaccggcg cctcgggttc ctgcgttggc
     1201 tcacccgcaa cggatacctc gcgggttgcc gcctgcccga gcccgtccgc cagaaagacc
     1261 ccgcccacga cgccgacccc agcgcacaga ccgacgccgc tcgcacgctc ttacacgacc
     1321 ccgacagcgc cagcatcgaa gaccgcgctg cggcctgcct gatcctgctc tacgcacaac
     1381 cggccgccaa gatcgtcacg ttgaccaccg acgacatcga ggtccgcgac ggtgacacct
     1441 acctcgccct cggccccgaa tccctgctgc tcgtcccacc actggacgcc ctcatcacgg
     1501 cgctgcccgt caccaagcta ttcggcaccg cgagcaccct ggccgatgtc cggtggctgt
     1561 tcaccgggaa gagtgccggc tcccacctgc atcccgcttc gttgatgcga aggatgcatc
     1621 gcctcggcat catcgcgcgc accggccgga ataccgcgct gctgcacctg gcctcgacca
     1681 cgccgccagc cgtgttcgcc agcctcatcg gcatccacgt caacaccgcg acccgctggg
     1741 ccgagatgtc gggctccgct tggaatgcct atgccgcaat ccgacgataa gaggactccc
     1801 gggcgaacac cgcttcaccc ccgtcggaca agcggggact gtcctcacgg accagcagct
     1861 cagaactcgc gcccctggtc tcccgggaac ggcggttcca tcgtcgcggt tgctgaaggc
     1921 caggaaaccg accaggacca gcactgcgac gccgatcatc gccagtgaca gctgcatgcc
     1981 cgactcaccg ctcacctccc gcgacggcaa gacggcccga aggtgatgcg acacccgctg
     2041 cgggcacggc atccttccgg ggcgtacggg cagcccgaag tccattggcg atcacgatca
     2101 cctcggcgac ctcgtggacc agcacgaccg cggcgaggcc gagcgcgccg gtgatcgcca
     2161 acggcagcag cacgacaatg atcgccagcg acagcacgat gttctggttg atgatcgccc
     2221 ggccgcggcg tgcgtgcagc agagcctgtg ggatcagcct gaggtcgtgg ccggtgaacg
     2281 cgacatcggc ggactggatc gccgcatccg acccggtcgc gcccatcgca atccccacgc
     2341 tcgctccagc cagggcgggg gcgtcgttga tgccatcgcc gatcatcgcc gtcggcgacc
     2401 gctcggccat ttctgtcacg atgcgggcct tgtcctctgg tcgcagctcg gcgtgcacgt
     2461 caacgatgcc ggcgatctgt gccagggcgg tggcggtgcg ggtgttgtcg ccggtgagca
     2521 tgctcacctc gacaccctga ccgcgcaggg tctcgatgac ttcgggcacc tcggaacgca
     2581 gttcgtcgcg gaccccgatc gcgccggcga cctcgtcatc aatagtgacg agcacgcagg
     2641 tctgccccgc agcctccaag tcctcgaccc gggacgcgag cggtccggga tcgatccagc
     2701 gggggctgcc cacccgcacc cggtgcccct cgacaatgcc ggtgatgcca tggccggctt
     2761 cctcggacac gtcccgcgcg gtcggcacgg cccctgcggc agcggcgatc gcggcagcca
     2821 gcggatgggt ggagtgctgc tcgatggcag ccgcagcggc caacaccttg tcctcgtcgg
     2881 tgccatcggc agggatcacc gccgtgacgg tgggttgatt gcgggtcagg gtgccggtct
     2941 tgtccaccgc caggtgccgg attccgccga ggcgctcgaa tgcagcgccg ctcttggtga
     3001 tgacaccgaa cttgctcgcc gccccgatcg ccgagaccac ggtgacgggt accgcgatcg
     3061 ccagcgcgca cggactggcc gcgaccagca ccaccagcgc ccgggtgatc cacaatcccg
     3121 gatcgccgaa cagcgacccg agcacaccga caaggacggc aagaaccatc acacccggca
     3181 ccagagggcg agcgatccgg tcagccatcc gcgctcgatc gcccttctcg gcctgggcct
     3241 gctcgaccag ttccacgatc gtcgtcagcg agttatcggt gcccgccgcg gtcgtctcga
     3301 cctccaacac tccggcggtg ttgatcgcac ccgcggagac ctcatcgccg ggctcgacct
     3361 cgaccgggat cgactcgccc gtgatcgccg atgtgtccaa actcgaccgc cccgaccgga
     3421 cgatcccatc ggtcgcgagg cgctctccag gtcggatcac catcacctgc ccgacgacga
     3481 tctccttcgc cgggatctcg acctggccac catcacgggt tatggtcgcc gtctcgggca
     3541 cgagattcag cagggcgcgc aggccaccac gggcccggtc catcgccttg tcctccaacg
     3601 cctccgcgat cgagtacaag aacgccagcg ccgctgcctc ctcgacgtag ccgaggatga
     3661 ccgcgccgac cgcgctgatt gtcatcaaca ggccgatacc cagcttgcgc ctggtgacaa
     3721 ggttcctgat cgcacccggc acgaacgtgt acgcgcccag cagcagaccc acccagaaca
     3781 ccaccagcgc ggcggtctcc agcccggacc attccaggac caggccggtg acgaacgcga
     3841 ccccggagaa gatcggcacc agcagggcag ggtcgcgcca ccacggacgc tcgacctcct
     3901 cggcctcacc cgcaggcttg gtggccggct cgtcgcaacc gcatgccgtg ctcacgacgc
     3961 atcctccgca gacccctgcg cgtcgcagca gcccgggacg gtgcaggccg ggtcgatgca
     4021 cggcacgttc tcatccaccg ccagcgtcac atctaccagc gccgtcagcg ccgccgccag
     4081 atgcgggtcg gcgatctcgt accgggtctg ccgcccctcg ggttcggaga ccacgatccc
     4141 gcagccccga agacacgtca gatggttcga cacgttcgtc cgcgtcagct ccagcttccg
     4201 agccagcttc gctggatacc ccggcccctc caacagcgac aatagaatcc tcgaccgcgt
     4261 tgggtcggcc atcgcccggc ccaatcggtt catcacatct acccgagaag caatagtcag
     4321 cacacactga ccatacagcg ctcagtgacc tatctccaaa ttgaatccca ctgactggca
     4381 tgagtttcga gcttgcttgg aattgtggtc gtcccatggt ccgccgcact ggccgagcct
     4441 gtggaacggc ccggccagcc gtgatgccga gtcgatcctc agcagtcgca gccgcacacc
     4501 tccggcgggc acatcgggtc ctcacagcag tcgttcatat caatcacctc cctcctttcg
     4561 cagccacgtc ccgggaatcg gctgcgggca gtcgtccgcg ggaagctcgc caagcatggc
     4621 gaccatctga gagcggagct cagccagtcg gaccatctcc tcatcaagct cggtgagccg
     4681 ccgggcgagc aactctcccg ccggctcaca cgggcacgtt cccgtgtcac gcacagccaa
     4741 caggtcgcgg atatcctcca gcctgagccc caaacgctgc gcgccctgga tgaaccgcag
     4801 ccgatccacc gcgccggcgt catactgccg gtacccggca gccgttcgct ccggcggcgg
     4861 aagaagcccg gcccgctcgt aataccgaat cgtgtccggc ctgaccccca cgacactggc
     4921 cagctccgcg acgcgcatcc gcggattcgg ctcaacatca actgacataa ccccacggta
     4981 aaccttgaag ccaactccaa gatcaagccc tcgggtccgc ccgctttccc acgacccggt
     5041 ggtccaactg gccagagcag ccaagatcaa cctgtctgag ccccgcgcgc cactgtcacg
     5101 gatcgaccgg cgcgggaccg cgttcgaccg ttcggtctca cgagccgccg gcggctcgga
     5161 ccggccaacg ttccggcatc accaaccgca agccgcacca ccgtcctgct gcacttcgtc
     5221 tcgaccatcc cgccgacgcc accgcgaccc gctgggccga actttccgtc gccgctagga
     5281 acgcctgcgc gatccctcac gcggacacca aggcacctgg acgaaccgtt gcagccaact
     5341 caccgaccct ggctttacgc gccggtgata atgccgtcgt gcgacactag agaaacgccc
     5401 ctcgcggcgc ccggtggtgg ggctcaccgg aaccaacctc acggacgtga caacggtccc
     5461 tatctcgaca ttgcgcgcca cggcgcgcct gtgggatagg agactgccga tgacagacga
     5521 gttgtcagcc aacgaacctc tcgacaccga cgccgtcgct gataccggac caggcctgcg
     5581 cggcatcgga ttggttgccc tcggcggaac ggccgggaca ctggcccgcc acgccctcga
     5641 cgcgctcatc ggagaaacgg ccggactccc ggtgggcatc ttcatcatca acatcaccgg
     5701 cgccttcgca ctcggtgtcc tcatcgagtc cctagctctc ggcggaccag accgcggacg
     5761 acgacgcgac ctacgcctgc tgctgggcgc cggactgctc ggcggttaca ccaccttcag
     5821 caccgccagc ctcgaaagca tccgcctgct caccgccaag cgctacaccg cagcactgct
     5881 caccggaccc ggcatgctca tcgcctgcac cacgctgtcg gtcgccggca tcctcatcat
     5941 cgcgccgtaa cacccgccaa cagcagtcgg cactccaccc ggcggcgggc atcagcaacc
     6001 agcggggccg tccacgcgca cctcaccatc atggatttcg aacgcttcga atacttgctt
     6061 ccgccagttt aatctcggaa gttgcatcta atgcacgatt catgcacttg ctacacgacg
     6121 gaatcacgtc gatctgcggc ggcgcggcaa gtccgagttt agcgcgtgac ccgctcctga
     6181 cctggcgttt cggggcctgg attgcatcag atgcatgaat cgtgcatcta atgtaaggcc
     6241 cgacgcgttc agcaggacga ggcggctccg gcccgcaatg cttgcgggtc gagcgcgccg
     6301 gagagcatcg ccagcgtctc ggggatgatc gtgtagtacg cccacttgcc gcgctgctcg
     6361 cgggtgacca ggccggcgtc gacgagctgc ttcatgtgat gcgacacggt gggctgggac
     6421 agtccgaccg gctcggtcag gtcgcacacg cacgcctccc cgccggactg cgcggcgatc
     6481 atcgacaaca gcctgaccct ggtcggatcg cccagcgcct tgaacacccg agccaggcgc
     6541 tgcgcgtcct cgacttccat cacgcccgca gtcaccggcg cgcagcaccc ggacgcatcc
     6601 gtggccagca gaggcagcga agtcgtcatg gctccactct accctgacgt attgacaggt
     6661 gtcgatgcat cgaccatcat caatatgagt tgatccgttc cgacgaagga gaccgctatg
     6721 ggcacgaaca acgacacggc cgagaaggag gagctgcgtg agcaggtccg cgcccgctac
     6781 gccggggccg cgaccgcggt caaggccggc gcccgcaacg agaacctgct ggtcgaggac
     6841 gcctgctgcg gcacgtcctg ctgcggcggt gcgcccgccg accgcgacac cacccagcct
     6901 gcggccgagg atgcctgttg cgactcgtcg tgctgcggcg acacgaccgc gacggagcgg
     6961 agcttcggcg cgggcctcta cgacgccgcg accaccgccg ggctgccgat cgaggcggtc
     7021 gaggcgagcc ttggctgcgg gaacccgacc gcggtgacag agctgcacga gggcgaacga
     7081 gtcctcgacc tcggctcggg cggcggcatc gacgtgctgc tctcggccag ccgcgtcggg
     7141 ccgacggggt tcgcttacgg ggtggacatg accgacgaga tgctggacct cgcccgcgag
     7201 aacgcagcca aagccgaggc cacgaacgtg gagttcttga agggcaccat cgaggacgtg
     7261 cccctgccga acgcctcggt ggacgtggtg atctccaact gcgttatcaa cctctccacc
     7321 gacaagcccg ccgtgctcgc cgagatgttc cgcgtcctca cccccggcgg acggatcggg
     7381 atctccgatg tcgtcgccga agaccacctg agccacgagc agcgcgccga gcgcggctcc
     7441 tacgtcggct gcatcgccgg agccctctcc aagagcgaat acctcgacgg gctcgccgcg
     7501 gtcgggttca ccgacgcgaa cgtggagttc acccacgaag ccgccgacgg catgcactcc
     7561 gccatcatcc gcgccaccaa gcccgccgcg tgaccacacc gcacggcgtg agcaccgcca
     7621 cggccccggt cgtcgcgggg gctcggcggt tgtcgaggct ggatgcctgg ctaccggcct
     7681 ggatcgggct cgcgatgatc gccggcctcc tgctcggccg attcgtgccc gccctctccg
     7741 acctgctcac ccgcatggag gtcgccggca tctccgtgcc gatcggcctt ggtctgctgg
     7801 tgatgatgta tccggtgctg gcgaaggtcc gctacgacaa ggtggccgcc gtcaccggcg
     7861 acaagaaact gctcgtgtcc tcgctggtgc tgaactggct ggtcggcccg gcagtgatgt
     7921 tcaccctcgc ctggctgttc ctgcccgacc tgcccgaata ccgcaccggg ctcatcatcg
     7981 tcggcctcgc ccgctgcatc gcgatggtcg tgatctggaa cgacctcgcc tgcggcgacc
     8041 gcgaagccac cgccgtgctc gtcgcgatca actcgatctt ccaagtcatc gcgttctcgt
     8101 tcctcggctg gttctacctc accctgcttc ccggctggct cggcctggac acccagggcc
     8161 tcgacgtgtc ggtcggaaag atcgcgctca acgtcctggt cttcctcggc atcccgctca
     8221 tcgccgggtt cgcgtcccgc tggatcggcg agcaccgccg aggccgggcc tggtatgagg
     8281 acacgttcct cccgcgcatc ggaccgtggg cgctctacgg gctgctgttc accatcgttc
     8341 tgctgttcgc cctccaaggc gaccagatca tcagccagcc gctcgacgtc gcccgcatcg
     8401 ccctgccgct gctggtctac ttcgcgctga tgtggttcgc cggcctcggg ctcggcaaga
     8461 gcctcggcct cggctacgcc cgctccacaa ccctggcgtt caccgcggcg ggcaacaact
     8521 tcgagctcgc catcgccgtc gcgatcggca ccttcggcgc caccagcggg caagccctcg
     8581 ccggggtcgt cggcccgctc atcgaagtgc ccgtcctcgt cgccctcgtc tacgtctccc
     8641 tctggaccgc caggcgctgg ttccacaccg atccctacca acccgaaacg agcccacgat
     8701 gaccacgacc aagccgaccg tcctgttcgt ctgcgtccac aacgccggcc gctcccagat
     8761 ggccgccggc ctgctcaccc acctggccgg ggaccgcatc gaggtccgct ccgccgggtc
     8821 cgagcccgcc gaccaactca accccgccgt catcgccgcc atgcgcgagg tcggcatcga
     8881 catcaccgcc gaacgcccca agctgctcac caccgacgcc gtgagaacag ccgatgtggt
     8941 catcacaatg ggctgcggcg acacctgccc gatcttcccc ggcaaacgct acgaagactg
     9001 gcagctcgac gaccccgccg gacaaggcat cgaggccgtc cgaccgatcc gagacgacat
     9061 cgaggcccgc gtccgcgacc tcatcaccca gctcacctga ccgagccgcg ccgtcgcagg
     9121 agcgcccgac gcacctgcac ggcatgaagc tcgcaacact caccaggctt catcgaagcg
     9181 tggaaccccg gctattccgg cacttcgatc atccgactct gccggtggct gtaccagcgt
     9241 tacatcagtc agtgacgcgg agtgagggct gatcgatggc ccatttccac gagccgtcag
     9301 gctggcgacg ggcgacctcg gcggtgacga aagcacctgg gacacgggtg gaggtaaagg
     9361 cgaggtcgcc gttgaccagt ggctccgctt gctggcccag cgatacctgc ggggcggtag
     9421 cgaggaattc tgaccagaac tctcggatcg cggccgaacc tacaaccggg ccgttgctgg
     9481 tatccaggat ggcgtcgtcc tcgtagaggg cgacgagccc ggcgaggtct cggctgttga
     9541 tgcgctcaac gagcagcgcc gagagctggt tgggttcggc tgccggttcg atagccatgc
     9601 gtctggtccc gtctcgaagg tcgttgtcgt caacgctatc gggcgggacc gacacgaccg
     9661 gaacagaagc caaccggcgc tggccaccgc gccgagttgc ctaccgcgca ccttacggtc
     9721 atggatctcg aacccttcgg atacttgcct tcgccggtcg gcggtgcgtg atggacgtgt
     9781 tccccgactt cgacgggctc ggcggcatcg gtgacctccg cgccgtggtc ggcgcgctct
     9841 tgacgttcgt cctgatcgtc gccgtcctca tgctgatcgt ctcggcgatc gtgtgggcca
     9901 tcgcgacggc ccacggcaac tacgccaccg ccagcaaggg ccgaatcgga gtcctcgtcg
     9961 ccgtcggtgc cgccgtcctc gccggaggtg gcgtggcctg gatgaactgg ctgctcacag
    10021 tcgggtcgag tttgtagctg cagcgttccc gtcattggtc cgagtcctcc gcgtcagcgt
    10081 tcgcggtctc gcaccgatca agcaggcgcg cgaggtagct gtacgccgcg aggcgctcca
    10141 tcgcttcctg ctcgg
//
LOCUS       DAOPRQ010000082        10135 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_5571_length_10135_cov_10.779762, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000082 DAOPRQ010000000
VERSION     DAOPRQ010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 10135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10135
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5571_length_10135_cov_10.779762"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /locus_tag="PKJ61_10095"
     CDS             complement(<1..592)
                     /locus_tag="PKJ61_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOC14212.1"
                     /translation="MPKSLNVNPAEVRKASTITVPPIPVNAYVQDFAAAKQRYGADGL
                     VAMLHDMLAIREFETMLNSIKTTGGWQGIEYNHKGPAHLSMGQEASAVGQAAELDADD
                     YIFGSHRSHGEILAKSFSAARKLDPAKLQGIMSTFLDGDTLRYAEKIGYDSAEELTEN
                     FILFGTLAEIFARKVGFNRGMGGSMHAFFTPFGSMPN"
     gene            complement(623..1990)
                     /gene="lpdA"
                     /locus_tag="PKJ61_10100"
     CDS             complement(623..1990)
                     /gene="lpdA"
                     /locus_tag="PKJ61_10100"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007587379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HOC14213.1"
                     /translation="MADFDVIVLGGGPGGYLAAERLGHAGKKVLLVEKEFLGGTCLNV
                     GCIPTKSLLNSAKLYSHALHSEQFGVTATGVSYDWAKVQAWKADVVSKLVAGVAATEK
                     RNGVEVVFGHGTLIGAGVVEVAGERKTADHVIIATGSVPVMPPIPGAKDNPLVVDSTG
                     LLAVAAVPKRLVVIGGGVIGVEFASLFAAFGAQVEVVEMLPEIVPFMDDDLAGRLRKA
                     LKDVTFRLGCRVTGIEGGTVVYTDAKGNEQRAEADLVLMAVGRRPLVDGWGAKEAGLE
                     AGPRGVLVNDTMRTNLPNVWAVGDVTGRSLLAHAAYRMGEIAAAHIIDPAGAKRAGQV
                     LRVDTIPWAVYSMPEAAGVGLTEAQAKARGHQVVTATVPLAVSGRAVAEQGVRGPGAV
                     KLIAEAATRAILGIHMLGNYAPETIWGAAAVLETELSVDDLRQVVFPHPTVSEAIREA
                     AWAIH"
     gene            complement(2019..3416)
                     /locus_tag="PKJ61_10105"
     CDS             complement(2019..3416)
                     /locus_tag="PKJ61_10105"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HOC14214.1"
                     /translation="MAQVVVMPQLGNTVESCLVTTWLVKVGDVVDAATLLCEIETDKS
                     AMEVPSGVAGTVLALLAEEGDDVPVKEPLVIVGEPGENIEELLAGAGLPPVTTGEAGE
                     VNESVPEPLTPAVSASAAAPAAPAVARSDEDSAVSPRARGLAASQGIAAEQLAGTGPQ
                     GRVIERDVQSAIASGAALTPGARAAAEGFVPGAAGTGIGGRVTRADLAAPVAESAEKS
                     PAVSTGSTSRATAEFPGEFTDIPLKGIRKVIAERMMNSLATSAQLTYTATAPASGLLK
                     LRKRLKNAADPELAAVTIGDLVGYATVKTLAKHAVVNAHLAEGTLRKFANVHLGMAVD
                     TPRGLLVPTLRFADTLTLREFSVQSKALANAAIGGSISPDLLSGATFTVSNLGSFGIE
                     SFTPLLNVPQVAILGVDAIFPRAVINADGSVGAEQRIGFSLTADHQVIDGADAARYLK
                     DLCAAIADIDLTVLG"
     gene            complement(3432..4187)
                     /locus_tag="PKJ61_10110"
     CDS             complement(3432..4187)
                     /locus_tag="PKJ61_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006680218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HOC14215.1"
                     /translation="MTIERTERESYILGQLARDGFLTVSGLSRNLKVSEVTIRTDLRN
                     LEERGVLVRTHGGAEATGYRTVLERQLQHAGEKDRIAAAAAELVRDFDHIMIEAGTTC
                     AQIVTHLSGRKGVQILTNSTLVLRQARLNPALTVILVGGAFHRPSESLVGPVTLRNLA
                     DFNVRLAFFGTDGFSVAGGLTTGFAEGADVIRAMQARAVESWLVADSAKYDQAGFVAV
                     SDLGALTGVITDVGLPADAQSDLNSSGLEVRYV"
     assembly_gap    4217..4316
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4317..5935)
                     /locus_tag="PKJ61_10115"
     CDS             complement(<4317..5935)
                     /locus_tag="PKJ61_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HOC14216.1"
                     /translation="MTDFPSTQYAIQFTGKDAVVVNPAKPVDPIGPTQILLKVEACGI
                     CFSDTKLLHAFEDHPRKSPVVAGLSADELAQVPSYRPDGQPTVPGHEPVVRVVAVGDQ
                     VTHYRVGERLLVQADWRHLPTAASNGAFGYNFEGALQEYVVVDERIVVHSGEEFMLRV
                     SDAPTAAQVALVEPWSTVEASYSRAERNTLAAGGRLLVVADAGVVPSGLAPLLAAARP
                     GSITVVGADPASVDPQAVASTKDALEAGWDDIVYFGSDAATIEALGALLSARSLLCVV
                     LGGARIDRRVKLDVGRVHYDFTRYIGTVGSDPAEAFTHVPANGEIREADRVAIIGAAG
                     PMGLMHTVRTVSLGLAGVSVVGADVSDERLANLAAVVNPTAAERGVPFEVVNSSATPL
                     QPGYSYVTCLVPVPALLSQAVDLAGEGAIVNAFAGFPAGTLAELDLQGIIERGVFVIG
                     TSGSEMSDMRTLLDKLETQALDTSISLDAVCGMAGFQDAIDAVNNRTSGGKIMVYPSL
                     PDLGLIRLSELAEKLPAVAAAMDGTRWTKAA"
     gene            complement(5973..7964)
                     /locus_tag="PKJ61_10120"
     CDS             complement(5973..7964)
                     /locus_tag="PKJ61_10120"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOC14217.1"
                     /translation="MNLVAELIAVANAFGADPEYSRAGGGNASVKVDGVLRIKPSGVP
                     LATLTADDLVGLRNDVLLESLASEVPTEGDPVRVAAEKALVDAPGGRRPSVEILFHAL
                     IPDALVLHTHPLVANALTCNADAEALAARLLGDYAVFVPYTDPGVPLARKVAEVRDAY
                     TARTGRPAPGVTLLGNHGIIVSGNTAAELTERAQWLTATIAAAIEAGTVADQANVIPA
                     QAGIPATESALINIVAPTLRGLLGNPERLAVVTSDASELVRSVTTTPAGRAILADGPL
                     IPDQIVYAGSLPLVFVPGEGDVAAQLTAAVAEYAQTHGKNPIITVIPGVIAFGAGADL
                     ASAQTALATFTDALRVARDANRIGTVRCMDAAERGFIENWEAESYRKSVAAGGSAGRM
                     TGKVVMVTGAAQGFGLGITTELLDAGASVVLADLNIDLARQNATELSAKHGAGRAVAV
                     QVNVSDEQSQLDAIAEVVRIFGGLDVFISNAGVLRAEGVMTQPVADFDLVTNVNYRGY
                     FLGVRAAAPVLAAQHAARPEVLFDIIEINSKSGLEGSKRNFAYAGSKFGGIGLTQSFA
                     LELIEYGIKVNAICPGNYLDGPLWMDPERGLFVQYLRAGKVPGATTVAEVRAFYEAKV
                     PMGRGCLPSDLAKAVYYIVDQTYETGQAVPVTGGQNMLN"
     gene            complement(7961..9148)
                     /locus_tag="PKJ61_10125"
     CDS             complement(7961..9148)
                     /locus_tag="PKJ61_10125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14218.1"
                     /translation="MTDTAVRDSAQPIPVVNTHIHFPPNFSAFETVADVVATATAEGA
                     RAIGISNFFDQQVYADFAEQAAVAGIVPLYGLEFITLDDELSAAGIRVNDPANPGRMY
                     LCGKGIAPFREKSELASRVAAEIRSGNDERAEAMVAKLAAWFLRCGLDTGLTADAIAA
                     KVAAAGGVPVEWVSLQERHIARAFQQALFELPPAERAQVLEAAYGSASGVDLENPGAL
                     QGELRSRLIKVGTPGFVPEVPLAFADAYAYVLEMGGIPTYPTLADGVSPVCPFEEPAT
                     ELAAKLLERGIHAAELIPIRNTSACVDAYVKAFTEAGIVVMGGTEHNTPDRIPVEVAC
                     VDGPVSDFARQAFWEATCVVAAHQHQVAQGGMGYVDASGERTSATVAELVELGANLIG
                     VAK"
     gene            9364..9786
                     /locus_tag="PKJ61_10130"
     CDS             9364..9786
                     /locus_tag="PKJ61_10130"
                     /inference="COORDINATES: protein motif:HMM:NF016885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0048029 - monosaccharide binding [Evidence
                     IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU domain-containing protein"
                     /protein_id="HOC14219.1"
                     /translation="MLLNLDPLLTGQLLMALDEMGHGDGVVIADAHFTASKLAKKHLI
                     DLPGISSPRVLTAIRSVMVPDVYGPTLDLMESPDGLLPVQTELIEAAQLNGEPYRLIE
                     RFAFYDLAKEAELIIRTGETRTYGNALFRKGVTPVASA"
     gene            9783..>10135
                     /locus_tag="PKJ61_10135"
     CDS             9783..>10135
                     /locus_tag="PKJ61_10135"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOC14220.1"
                     /translation="MTATETAAVAGAHDPSLLLQVEGVEKTFPGVKALEGMRFDLRKG
                     EVLGLVGENGAGKSTMMKLLTGIYSREAGEFWLNGEPLNVHTPKEAQEAGLSIIHQEL
                     NLVSHLSVAENIWLGR"
BASE COUNT         1616 a         3421 c         3398 g         1600 t
ORIGIN      
        1 tgttgggcat cgagccgaac ggggtgaaga aggcgtgcat cgagccgccc atgccccggt
       61 tgaagcccac cttgcgggcg aagatctcgg ccagggtgcc gaacaggatg aagttctcgg
      121 tcagctcctc ggcgctgtcg tagccgatct tctcggcgta gcgcagggta tcgccgtcca
      181 ggaaggtgct catgatgccc tggagcttgg ccgggtcgag cttgcgcgcc gcggagaagc
      241 tcttggcgag gatctcaccg tggctgcggt ggctgccgaa gatgtagtcg tcggcgtcca
      301 attcagcggc ctgaccgacc gcggaggctt cctggcccat cgacaggtgc gccgggccct
      361 tgtggttgta ctcgatgccc tgccagccgc cggtggtctt gatcgagttc agcatggtct
      421 cgaactcgcg aatggccagc atgtcgtgga gcatcgcgac caggccgtcg gcgccgtagc
      481 gctgcttcgc ggcggcgaaa tcctggacgt aggcgttcac cgggatcggc ggcaccgtga
      541 tcgtcgaggc cttccggacc tcggccggat tcacattcag tgacttgggc atggttgttt
      601 ctgttcctct tcgtttgtca gatcagtgga tggcccaggc cgcttcgcgg atggcctcag
      661 agacggtggg atgcgggaag acgacctggc gcaggtcgtc cacgctcagt tcggtttcga
      721 ggacggctgc tgctccccag atggtttcgg gggcgtagtt gccgagcatg tggatgccga
      781 ggatggcgcg ggtggccgcc tcagcgatca gcttcaccgc acccgggcca cggacgccct
      841 gctcggcgac cgcgcgtccg gagacagcga gcgggacggt ggccgtgacc acctggtggc
      901 cgcgggcctt ggcctgggcc tcggtgagcc cgacgccggc agcctcgggc atcgagtaga
      961 ccgcccacgg gatggtgtcg acgcgcaaca cctgcccagc ccgcttggcc ccggccggat
     1021 cgatgatgtg cgccgcggcg atctcaccca tccggtaggc ggcgtgggcg agcaggctgc
     1081 gtccggtgac atcgccgacc gcccacacgt tcggcaggtt ggtgcgcatc gtgtcgttca
     1141 cgaggacccc gcgcgggccg gcttccaggc cggcctcctt cgcgccccag ccgtcgacca
     1201 gcggacgccg tccgaccgcc atcagcacca gatcggcctc ggcgcgctgc tcgttgccct
     1261 tcgcgtcggt atacacgacg gtgccgccct cgatgccggt gacccggcag ccgaggcgga
     1321 aggtgacgtc cttgagcgcc ttgcgcaggc gtccggccag gtcgtcgtcc atgaacggga
     1381 cgatctcggg caacatctcg accacctcga cctgcgcgcc gaacgccgcg aacaaggacg
     1441 cgaactcgac cccgatcaca ccgccaccga tcaccaccaa ccgcttcggc accgcggcga
     1501 ccgccagcag accggtggag tcgaccacca gcgggttgtc cttggcgccc gggatcggcg
     1561 gcatcaccgg caccgagccg gtggcgatga tcacgtggtc ggcggtcttt cgctcgccgg
     1621 ccacctcgac caccccggcg ccgatcaggg tgccgtggcc gaagaccacc tcgaccccgt
     1681 tgcgcttctc ggtcgccgcg acaccggcga ccagcttgga caccacgtcc gccttccacg
     1741 cctgcacctt ggcccagtcg tagctgaccc cggtcgcggt cacgccgaac tgctcactgt
     1801 gcagcgcgtg ggagtacaac ttcgccgagt tcagcagcga cttggtcgga atgcagccca
     1861 cgttcaggca ggtgccaccg agaaactcct tctcgaccag cagcaccttc ttgcccgcat
     1921 gccccagccg ctcggcagcc aggtagccgc cagggccacc gcccaggacg atcacgtcaa
     1981 agtcagccat cagatgttct tccttgtcag gattcggttc agccgagcac ggtcaggtcg
     2041 atgtcggcga tcgccgcgca caggtccttc aggtagcggg ctgcgtccgc tccgtcgatc
     2101 acctggtggt cggcggtcag cgagaagccg atccgctgct cagcacccac cgagccgtcg
     2161 gcgttgatca ccgcgcgggg gaagatcgcg tccacgccaa ggatggccac ctgcggcacg
     2221 ttcagcagcg gggtgaacga ctcgattccg aacgaaccca ggttgctcac cgtgaaggtg
     2281 gcgccgctga gcaggtcggg gctgatcgag ccaccgatcg ccgcgttcgc cagcgccttg
     2341 gactgcaccg agaactcccg cagcgtcaga gtgtcggcga agcggagcgt cggcaccagc
     2401 aggccgcgcg gggtgtcgac cgccatgccg agatggacgt tggcgaactt ccgcagggtg
     2461 ccctcggcca ggtgcgcgtt gacgaccgcg tgcttggcca gcgtcttcac cgtggcgtaa
     2521 ccgaccaggt cgccgatggt gaccgcggcc aactcgggat cggcggcgtt cttcagccgc
     2581 ttgcgcagct tgagcagacc ggacgccggc gcggtcgccg tgtaggtcag ctgtgccgag
     2641 gtggccaggc tgttcatcat ccgctcggcg atcaccttgc ggatgccctt cagcgggatg
     2701 tcggtgaact caccggggaa ctccgcggtc gctcggctgg tcgaccctgt cgagaccgca
     2761 ggggacttct cggccgactc agccaccgga gcagccagat cagcgcgggt cacccgtccg
     2821 ccgatccctg taccggcagc acccggcacg aagccctccg ccgcagcccg ggcgcccggg
     2881 gtcagcgcag caccggacgc gatcgcggac tgcacgtcgc gctcgatgac ccgtccctgc
     2941 ggaccggttc cggccaactg ctcggcagcg atcccctggg acgcggccag tccgcgagcc
     3001 cgcggcgaga ccgccgaatc ctcgtccgac ctggccacgg ccggcgcagc aggcgcagcc
     3061 gcggaagccg agacggccgg agtcagcggt tcaggaacgg actcgttcac ctcgccggcc
     3121 tcaccggtgg tcaccggcgg cagcccggcc ccggccagca gctcctcgat gttctccccc
     3181 ggctcgccga cgatcaccag cggctccttc accggcacgt cgtcgccctc ctcggcgagc
     3241 agcgccagca cggtgccggc cacgcccgag ggcacctcca tcgccgactt gtcggtctcg
     3301 atctcgcaca gcagcgtggc ggcgtccacc acatcgccga ccttgaccaa ccaggtcgtg
     3361 accaggcacg actccaccgt gttgcccagc tggggcatca ccactacctg cgccattgag
     3421 aactcccttt gctagacata gcggacttca aggccgctgc tgttgaggtc ggattgggcg
     3481 tcggcgggca ggccgacgtc cgtgatcaca ccggtgagcg cgccgaggtc ggagaccgcg
     3541 acgaaaccgg cctggtcgta cttggccgag tcagccacca gccatgactc gaccgcgcga
     3601 gcctgcatgg cccgaatcac gtccgcgcct tcggcgaagc cggtggtcag cccgccggcg
     3661 acgctgaagc cgtcggtacc gaagaaggcg agccggacat tgaagtcggc gaggttgcgc
     3721 aaggtcaccg gcccgaccag cgactcgctc ggacgatgga aagcgccgcc gacgaggatc
     3781 acggtcagcg ccgggttgag ccgcgcctgg cgcagcacca gcgtcgaatt ggtgaggatc
     3841 tggacgccct tgcgtccgct caggtgggtg acgatctggg cgcaggtggt gccggcctcg
     3901 atcatgatgt ggtcgaagtc gcggacgagt tcggcggcgg cagcggcgat gcggtccttt
     3961 tcgccggcat gctgcaactg ccgctccagc acggtgcggt agcccgtcgc ctcggcgccg
     4021 ccgtgggtgc gcaccaggac gccgcgctcc tcgagattgc gcaggtcagt gcggatcgtg
     4081 acttcggaga ccttgagatt gcgggagagc ccggagacgg tgaggaagcc gtcacgcgca
     4141 agctgaccga ggatgtacga ctcgcgctcc gtgcgctcga tcgtcatcga tcgtcctttc
     4201 gcttctcttt cggcttnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntcgg
     4321 cggccttggt ccagcgagtg ccgtccatgg cggcggcgac cgccgggagc ttctcggcga
     4381 gttcgctgag ccggatcagg ccgagatcgg gcaggctcgg gtagaccatg atcttgccgc
     4441 cggaggtgcg gttgttgacc gcgtcgatgg catcttggaa gcccgccatc ccgcagaccg
     4501 cgtccaggga gatcgaggtg tccagcgcct gggtctccag cttgtccagc agggtgcgca
     4561 tgtcgctcat ctccgagccg gaggtaccga tcacgaagac cccgcgctcg atgatgccct
     4621 gcaggtccag ttcggcaagg gtgccggccg ggaaaccagc gaaggcgttc acgatcgcac
     4681 cctcaccggc caggtccacg gcctgggaaa gcagcgcagg gacgggcacc aggcaggtca
     4741 catagctgta gccaggctgc agcggggtgg ccgaggagtt caccacctcg aacgggacgc
     4801 cgcgctcggc cgcggtcggg ttgaccaccg cggccaggtt cgccagccgc tcgtcactga
     4861 cgtccgcgcc gacgacgctc accccggcca gtccgagcga aacggtgcgc acggtgtgca
     4921 tcaaccccat cgggccggcc gcgccgatga tcgcgacccg gtcggcctcg cggatttcgc
     4981 cgttggccgg gacgtgggtg aacgcctcgg ccgggtcgct cccgacggtg ccgatgtagc
     5041 gggtgaagtc gtagtggacg cgaccgacgt ccagcttgac ccgccgatcg atcctcgctc
     5101 cgccgagcac cacgcagagc aggctgcgcg cactcagaag ggcgccgagt gcctcgatcg
     5161 tggccgcgtc cgaaccgaag tagacgatgt cgtcccagcc ggcctcgagc gcatccttcg
     5221 tcgaagccac agcctgtggg tcgaccgaag ccgggtcagc ccccaccacc gtgatcgaac
     5281 ccggacgggc cgccgcgagc aaaggcgcca acccggacgg gaccacgccg gcgtcggcca
     5341 ccaccagcag ccggccgccg gcagccagcg tgttgcgctc ggcgcgggag taggaggcct
     5401 ccactgtcga ccagggctcg accagcgcaa cctgcgcggc ggtgggcgcg tccgagaccc
     5461 gcagcatgaa ctcctcaccg gagtggacga cgatccgctc gtccaccacc acgtactcct
     5521 gcagggcgcc ctcgaagttg tagccgaagg cgccgttcga ggcggcggtc ggcaggtggc
     5581 gccagtcggc ctgcaccagc agccgctcgc ccacgcgata gtgggtcacc tgatcgccaa
     5641 cggcgaccac ccgcaccacc ggctcgtggc cgggcacggt cggctggccg tcgggacggt
     5701 agctgggcac ctgcgccaat tcgtcggcgg acaggccggc caccacgggc gacttgcgcg
     5761 ggtgatcctc gaaggcgtgc agcagcttgg tgtcggagaa gcagatgccg caggcctcga
     5821 ccttcagcag aatctgggtc gggccgatcg ggtccaccgg cttggcgggg ttgaccacca
     5881 cggcgtcctt gccggtgaac tggatggcgt actgggtgct cggaaagtcg gtcatgtctg
     5941 gttccttggg ttcgttgatc tcgcaggatc gctcagttga gcatgttctg gccgccggtc
     6001 accggcacgg cctgtccggt ttcgtaggtc tggtcgacga tgtagtacac agccttggcc
     6061 agatcggacg gcaggcagcc gcgtcccatc ggcaccttgg cctcgtagaa ggcgcgaacc
     6121 tcggcgaccg tggtcgcgcc gggcaccttg ccggcccgca ggtactggac gaagagcccg
     6181 cgctcgggat ccatccacag cggtccgtcc aggtagttgc ccgggcagat cgcgttgacc
     6241 ttgatgccgt actcgatcag ctccagcgcg aagctctggg tgagcccgat gccgccgaac
     6301 ttcgagcccg cgtaggcgaa gttccgcttc gacccttcca gccccgattt ggagttgatc
     6361 tcgatgatgt cgaacagaac ctctggacgt gcggcatgtt gcgccgcgag caccggcgcg
     6421 gccgcgcgga ccccaaggaa gtagccgcgg tagttcacgt tggtgaccag gtcgaaatcg
     6481 gccaccggct gggtcatcac cccctcggcc cgcagcacgc cggcattgga gatgaagacg
     6541 tccagcccgc cgaagattcg cacgacctcg gcgatcgcat ccagctggga ctgctcgtcg
     6601 gagacgttca cctgcaccgc gaccgcccgg cccgcgccgt gcttcgcgct gagttcggtc
     6661 gcgttctgcc tagccagatc aatgttcagg tcggcaagca cgacggacgc gccggcgtcc
     6721 agcagttcgg tggtgatgcc cagaccgaag ccctgcgcgg caccggtgac catgaccacc
     6781 ttgccggtca tccggccggc ggaaccgccg gcggccaccg acttgcggta gctctcggcc
     6841 tcccagttct cgatgaagcc gcgctcggcc gcgtccatgc agcggacggt gccgatgcgg
     6901 ttcgcgtccc tggccacccg aagggcgtcg gtgaaggtgg ccagggcggt ctgagcggac
     6961 gcgaggtcgg caccggcgcc gaacgcgatc acgccgggga tgaccgtgat gatcgggttc
     7021 ttgccgtggg tctgcgcata ctcggcgacg gccgccgtga gctgcgcggc gacatcgccc
     7081 tcgcccggga cgaagaccag cggcagcgac ccggcgtaga cgatctgatc ggggatcagc
     7141 gggccgtcgg ccaggatcgc ccgacccgcc ggcgtggtcg tgacgctgcg caccagctcg
     7201 gacgcgtccg aggtgacgac ggcgagccgc tcggggttgc ccagtaggcc gcgcagcgtg
     7261 ggtgcgacga tgttgatcag cgccgactcg gttgcgggga tcccggcctg cgccgggatg
     7321 acgttggcct ggtcagccac cgtcccggcc tcgatcgcgg cggcgatggt ggcggtgagc
     7381 cactgcgcgc gctcggtcag ctcggcggcg gtattgccag acacgatgat gccgtggttg
     7441 cccagcaggg tgacccccgg cgcgggccgg ccggtgcgcg cggtgtaggc gtcgcgcacc
     7501 tcggccacct tgcgggccag cggcacgccc ggatcggtgt agggcacgaa taccgcatag
     7561 tccccgagca gccgggcagc cagtgcctcg gcgtcggcgt tacaggtgag ggcattggcg
     7621 accagcgggt gggtgtgcag caccagcgcg tccgggatca aggcgtggaa caggatctca
     7681 acactcggac ggcgtccgcc gggagcgtcc accagcgcct tctccgcggc cacccgcacc
     7741 gggtcgccct cggtcggcac ctcggaggcg agcgactcca gcagcacgtc gttgcgcagt
     7801 ccgaccaggt cgtcggcggt cagcgtggcc agcggaaccc cggacggctt gatccgcagc
     7861 accccgtcca ccttcaccga agcgttgccg ccgccggcgc gggagtactc ggggtcggcg
     7921 ccgaaggcgt tggcgaccgc gatcagttcg gcaaccaggt tcacttcgca actccgatca
     7981 ggttcgcacc gagctcgacc agctcggcga cagtcgccga cgtgcgttcg ccggacgcgt
     8041 ccacatatcc catcccaccc tgagcgacct ggtgctgatg cgcggcgacc acgcaggtgg
     8101 cctcccagaa cgcttggcgg gcgaagtcgg aaaccgggcc gtccacgcag gccacctcca
     8161 ccgggatccg gtcgggggtg ttgtgctcgg tgccgcccat cacgacgatc ccggcttcgg
     8221 tgaaggcctt cacataggcg tccacgcagg cggaggtgtt ccggatcgga atcaactcag
     8281 cggcgtggat gccgcgctcc aacagcttcg cggcgagctc ggtggccggc tcctcgaacg
     8341 ggcagaccgg gctcactccg tccgcaagcg tgggataggt ggggatgccg cccatctcca
     8401 ggacgtaggc gtaggcgtcc gcgaaagcca agggcacctc cggcacgaag cccggcgtac
     8461 ccaccttgat cagcctggaa cgcagctcgc cctgcagcgc acccgggttc tccaggtcga
     8521 ccccggaagc cgaaccgtac gcagcttcga gcacttgagc acgttcagca ggcggcaact
     8581 cgaacaacgc ctgctggaag gcccgcgcaa tgtgccgctc ctgcagcgaa acccattcga
     8641 ccgggacgcc tcctgcggcg gcgaccttcg ccgcgatcgc gtccgcggtc agtccggtgt
     8701 ccaggccaca gcgaaggaac caggcagcca gcttggcaac catcgcctcg gcccgctcgt
     8761 cgttacccga cctgatctca gcggccactc gcgatgccag ctccgacttc tcgcggaacg
     8821 gcgcgatgcc cttgccgcac aggtacatcc ggcccggatt ggccgggtcg ttcacccgga
     8881 tgcccgcagc tgagagttcg tcgtcgaggg tgatgaactc gaggccgtag agcggcacga
     8941 tgcccgcgac cgctgcctgt tcggcgaagt cggcgtagac ctgctggtcg aagaagttgg
     9001 agatgccgat cgctctcgcg ccctcagcgg tggcggtggc aaccacgtcg gccacagtct
     9061 cgaaggcgga gaagttcggc gggaagtgga tgtgcgtgtt caccactgga atcggctgag
     9121 ctgagtcccg gacggcggtg tcggtcatgg cctacctgtt cttggttctg gacggtttct
     9181 tcacgtcttt ttggaacgat tcaagagcct agcagttgcg tgcgtcgagc actagccccc
     9241 gacgctcgcc ggctgcccac aacggcccct agtctcgaaa aagataacga tttcatatcg
     9301 tcgcgtgaca atcggtacct acccgcccta gcatacagaa atcgattgca gacggaggcg
     9361 atgatgctgc tcaatctgga tccgttgctt acgggccagc tgctgatggc gttggacgaa
     9421 atgggccatg gcgatggtgt cgtgatcgca gatgcccact tcaccgcgtc caagctggcc
     9481 aagaagcacc tgatcgacct acccggaatc agctcaccca gagtgctgac cgcgatccgc
     9541 tcggtgatgg tgcccgatgt ctacggcccc acactcgatc tgatggagag cccggacggg
     9601 ctactgcctg tgcagaccga actgatcgag gccgcccaac tgaacggcga gccctaccgg
     9661 ctgatcgagc ggttcgcctt ctacgacctg gccaaggagg ccgaactgat catccgaacg
     9721 ggtgagaccc gcacgtatgg caacgcacta ttccgcaaag gcgtgacccc ggtggcttcg
     9781 gcatgaccgc caccgagact gcggctgtgg ccggcgcgca cgacccgagc ctgttgttgc
     9841 aggtggaggg tgtggagaag accttccctg gcgtgaaggc tctggagggc atgcgtttcg
     9901 accttcgcaa gggtgaggtg ctcggcctgg tcggggagaa cggtgccggc aagtcaacga
     9961 tgatgaagct gctgaccggt atttactctc gtgaggctgg cgaattctgg ttgaacggtg
    10021 agccgctgaa cgtgcacacg ccgaaagagg cgcaggaagc cgggttgtcg atcatccacc
    10081 aggaactcaa cctggtctcg cacttgagcg ttgccgagaa catctggttg ggtcg
//
LOCUS       DAOPRQ010000083        10108 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_5587_length_10108_cov_11.843330, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000083 DAOPRQ010000000
VERSION     DAOPRQ010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 10108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10108
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5587_length_10108_cov_11.843330"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..884
                     /gene="atpD"
                     /locus_tag="PKJ61_10140"
     CDS             <1..884
                     /gene="atpD"
                     /locus_tag="PKJ61_10140"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016667716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HOC14221.1"
                     /translation="GTSVFAGVGERTREGNDLINEMIEAGVMKDTALVFGQMDEPPGT
                     RLRVALSALTMAEYFRDVQGQDVLLFIDNIFRFTQAGSEVSTLLGRMPSAVGYQPNLA
                     DEMGQLQERITSTRGHSITSMQAIYVPADDYTDPAPATTFAHLDATTELSREIASRGL
                     YPAVDPLSSSSRIMDPQFIGQDHYDTATRVKKILQRNKELQDIIAILGVDELPEEDKI
                     IVSRARRIQQFLSQNTYMAEKFTNVPGSTVPLADTIESFKMICDGEVDHVAEQAFFNV
                     GGMDMVMANWDRIQKEG"
     gene            886..1293
                     /locus_tag="PKJ61_10145"
     CDS             886..1293
                     /locus_tag="PKJ61_10145"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HOC14222.1"
                     /translation="MAVLRVEVVAAEGIIWEGDALSVIARTVEGDLGILPNHEPFLAV
                     LVPCAAEVLTAEGNREVLAIDGGFISVADNRVSLLSQFAKVAHEIDLNGAEHELAAAE
                     KRLNAGELDEETRQHFLRAQAQVRAARMAQQNH"
     gene            1311..1751
                     /locus_tag="PKJ61_10150"
     CDS             1311..1751
                     /locus_tag="PKJ61_10150"
                     /inference="COORDINATES: protein motif:HMM:NF022199.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2550 domain-containing protein"
                     /protein_id="HOC14223.1"
                     /translation="MDWLGVTEVVALVLVVALLVPLVSLYARRRWLARQGGLFDCSLR
                     LSTKTPGTGWVLGVARYTGDNLEWFRSFSLSFRPRLVFPRSQSRTGAQREPDPIESVL
                     LYDDQRIVRMELTDGSSWELAMSVASLTGLLSWLESSPPGSRYR"
     gene            complement(1866..2270)
                     /locus_tag="PKJ61_10155"
     CDS             complement(1866..2270)
                     /locus_tag="PKJ61_10155"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HOC14224.1"
                     /translation="MRTTRNRERGAYVYFMTNRCDSVIYIGVTNDLLRRVAEHKLGMG
                     GFTARYRCTKLVYFEAGDSISDAVAREKQLKNWRRAWKNRLVSESNPGWVDLGPRIGL
                     SAELLARIATDSAFGGEEDAPASCREDGSAVS"
     gene            complement(2612..3265)
                     /locus_tag="PKJ61_10160"
     CDS             complement(2612..3265)
                     /locus_tag="PKJ61_10160"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOC14225.1"
                     /translation="MVNLTRIYTRTGDAGRTRLSDMSETSKTDPRVEAYGHVDEANSL
                     LGLVVVQSDLDDDFRAVLRHIQNELFDCGADLSNPLMLHSSYEPLRVIQANIDRLEGW
                     CDQFGDPLPALRSFILPGGTQTAALLHLARSVVRRAERAGWTAVEIYGTEPAEGHQPG
                     GVNLLALTYLNRLSDLLFILARAAGQQAGEVLWIPGKDREVTDPRAAKQRRRIQKTE"
     gene            3364..4071
                     /locus_tag="PKJ61_10165"
     CDS             3364..4071
                     /locus_tag="PKJ61_10165"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOC14226.1"
                     /translation="MKKLILLLVASVLALAGCSSGAVRGEPAGASSPVVSSSASASAT
                     TAGEVSCTYTATNDGVKKVDLPPTDGVAATGTAEYVLTLNSDPLKLTLDRENAPCTVN
                     SFSSLAGQGFFDGSSCHRLVDDGGLYVLQCGDPSGTGSGGPGYQFDDELTGSESYGAG
                     TLAMANAGPGTNGSQFFIVYADSQLSPDYTVFGHVDEAAVKVLAKLAAGGQDNSFGSS
                     GGGKPNSPAVITSIKPA"
     assembly_gap    4119..4218
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4268..5080)
                     /locus_tag="PKJ61_10170"
     CDS             complement(4268..5080)
                     /locus_tag="PKJ61_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017935530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOC14227.1"
                     /translation="MLAAAALMARLHTELVGSGRPRFAFLPGLFGRGRNWSQIAQRLA
                     EHELASVLFDLPDHGQSAWTDHFSYLGMAEAVADEVDLRLGSAAQLVLVGHSMGGKVA
                     MLTALRRPELASGLAVIDIAPGHSDQVETFTPLVAAMRGLDLSTIDSRAEADARLSPA
                     VPDDAVRLFLMQNLRRRHGWHWQPNLRLLGDSLDAIGGWPDPGPATYPGPVLWLTGEQ
                     SAYVRAEHEADMARLFPDVERVVVPGAGHWVHADDPDATVAALLELEARTHR"
     gene            complement(5059..5334)
                     /locus_tag="PKJ61_10175"
     CDS             complement(5059..5334)
                     /locus_tag="PKJ61_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14228.1"
                     /translation="MAKRPSKHLRAPRPLSTGAFAQLEIKADGEWMVQTMPADAAGKT
                     YTCPGCSRQVAPGASHLVAWPRLASIGSTSAVDERRHWHTACWQRRR"
     gene            5404..6114
                     /gene="nucS"
                     /locus_tag="PKJ61_10180"
     CDS             5404..6114
                     /gene="nucS"
                     /locus_tag="PKJ61_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease NucS"
                     /protein_id="HOC14229.1"
                     /translation="MRLLIARCQVDYAGRLTAHLPMATRLIMVKADGSVSVHADDRAY
                     KPLNWMSAPCTLTVSPPPEDVEAVAEADEAGLTEVWEVRNTDGDTLQIALGEVLHDSN
                     HELGIDPGLQKDGVEAHLQVLLAENPRTFGEGWTLIQREYLTPIGPVDLLLRDHTGAY
                     VAVEVKRRGEIDGVEQLTRYLALMNADPTMSPVRGVFAAQQIKPQARTLAGTRGIECV
                     LVDYNVLRGLDNAEDRLF"
     gene            complement(6125..7363)
                     /locus_tag="PKJ61_10185"
     CDS             complement(6125..7363)
                     /locus_tag="PKJ61_10185"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOC14230.1"
                     /translation="MTTPGSEPQPPAPPGGVPVPASARTPAALSAHTPTSAPPTRGWL
                     RKWRRTTQPTPADADGRRLGASDRISLLDRNPFQIGFFLTFGALAATGLVYTVISLRT
                     VLVLLMLSLAIALGLNPLVDWLHHHGVRRAFAVLLVALSAIGLLSLAVWALVPVVSDQ
                     VNKLMLQAPDYLENLRQNPQIAAFDAQFHVIEKAVEFLTSGELISSLFGGLLGAGQAV
                     ANTIFSVVITLVLTLYFLTSLPNLKEVIYKLAPASRRPRVKYLANEMFKRVSGYVSGL
                     FIVVLIAMSVAFVFLNVIGLSQYALALTVVVGLFAFVPLVGTTISMVIVSIIAFSFSP
                     TTGLITLIFFLAYQQFDAYFIQPRVFSHSVQVPSVLVMLAAISGYLLQGMIGAILAIP
                     VMAALLLLYREVLLPHLDRT"
     gene            complement(7360..8391)
                     /locus_tag="PKJ61_10190"
     CDS             complement(7360..8391)
                     /locus_tag="PKJ61_10190"
                     /inference="COORDINATES: protein motif:HMM:NF016959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HOC14231.1"
                     /translation="MVGEDTTGLELFDEVASAVGNFPQSLRGYDRGAVDAYVRDVEGQ
                     LARAKAQLRQQHRQLAEANARAADSDFGKAGAHTRTMLKAAESQAGELVDSADSRARL
                     VLSAAEAEARRLTIEAELALDAARAASIQELDALRQRLSEQTAAELEAAKQEANAVRQ
                     AAELHASQVVADAQARAAAIAEGARIDAAARAAEVERAAAEARLRLAERKEADLGELK
                     AAQAANATRLDDLLAEAEAVSTDYRTRLEADAKTWNERRAAALAEAEQIIASARAEAE
                     GIASAAEHEGERIRAERTREAEVEVERLQAELKLLIGRRQSVVEQLGELANLVGVSVA
                     DYTDGESGS"
     gene            complement(8469..8927)
                     /gene="mce"
                     /locus_tag="PKJ61_10195"
     CDS             complement(8469..8927)
                     /gene="mce"
                     /locus_tag="PKJ61_10195"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HOC14232.1"
                     /translation="MDNMSNDDLFICIDHVGYAVHNLDDAIKFHTEVLGWRVLHRETN
                     AEQGVEEAMIGTGDQLTENAQVQLLAPLNENSTIAKWIERNGGKGGIQQVAYRVADLD
                     KVSAVLRERGVRLLYDAPKIGTGGSRINFAHPKDVGGILLEIVEPAAAAH"
     gene            complement(8942..>10108)
                     /locus_tag="PKJ61_10200"
     CDS             complement(8942..>10108)
                     /locus_tag="PKJ61_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002527249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase"
                     /protein_id="HOC14233.1"
                     /translation="FKYNPPHGGPADTDATSVIAARANELLRSVGSIKRTPYATAARD
                     VTRVDYLGAYCEDLRNALDLDAIASAGVRIGADPLGGASVQYWGYIAERLGIDLTVIN
                     PQVDPAWPFMTLDWDGKIRMDCSSPYAMASLIANRESYEISTGNDADADRHGIVTPDY
                     GLMNPNAYLAVAIQYLFGHRPNWRPDAGVGKTLVSSSLIDKVAADLGRRLVEVPVGFK
                     WFVPGLMSGDLGFGGEESAGASFLARDGSTWTTDKDGILLALLASEIKAVTGKSPSEL
                     YAELEARHGASSYARVDAPASREQKAKLAKLSASDVTATELAGEPIVATLTHAPGNGA
                     AIGGLKVATASAWFAARPSGTENVYKIYAESLRGPEHLAQVQAEAKEVVDGVLS"
BASE COUNT         1651 a         3280 c         3387 g         1690 t
ORIGIN      
        1 gtggcacctc ggtgttcgcc ggcgtggggg agcgcacccg tgagggcaac gacctgatca
       61 acgagatgat cgaggccggc gtcatgaagg acaccgcgct ggtcttcggc cagatggacg
      121 agccgccggg cacccgcctt cgggtggcgc tgagtgcgtt gaccatggcg gagtacttcc
      181 gcgacgtgca gggccaggac gtgctgctct tcatcgacaa catcttccgg ttcacccagg
      241 ccggttcgga ggtgtccacg ttgctcggcc ggatgccgtc cgcggtgggc taccagccga
      301 accttgccga cgagatgggc cagctgcagg agcggatcac ctcgacccgc ggccactcga
      361 tcacgtccat gcaggccatc tacgtgcccg ccgacgacta caccgacccg gcgccggcaa
      421 ccaccttcgc gcacctggac gccaccaccg agttgagccg cgagatcgcg tcccgcggtc
      481 tctacccggc cgtcgacccg ctgtcctcgt ccagccggat catggacccg cagttcatcg
      541 gccaggacca ctacgacacg gccacccggg tgaagaagat cctgcagcgc aacaaggagc
      601 tccaggacat catcgcgatc ctaggtgtcg acgagctgcc cgaagaggac aagatcatcg
      661 tctccagggc gcgccggatt cagcagttcc tgagccagaa cacgtacatg gccgagaagt
      721 tcaccaacgt gcccggttcg accgtgccgc tggcggacac catcgagtcg ttcaagatga
      781 tctgtgacgg cgaggtcgac cacgtcgctg agcaggcctt cttcaacgtc ggtggcatgg
      841 acatggtgat ggccaactgg gaccggattc agaaggaagg ctgacgtggc ggttctgcgc
      901 gtcgaggtcg ttgcggccga ggggatcatc tgggaaggcg atgccctcag cgtgatcgcc
      961 cggacggtcg aaggcgatct cggtatcctg ccgaaccacg agcctttcct tgcggtgctg
     1021 gtgccgtgtg cggccgaagt gctgactgcg gagggaaacc gagaggtgct ggcgatcgac
     1081 ggcggcttca tctcggtcgc ggacaatcgg gtttcgctgc tcagccagtt cgccaaggtc
     1141 gcccacgaga tcgacctgaa cggggccgaa cacgagttgg cggccgcgga gaagcggttg
     1201 aacgccggtg agctcgacga ggagacccgg cagcacttcc tgcgggcgca ggcgcaggta
     1261 cgcgctgcgc gcatggccca gcagaaccac tgaggcgggc cgggcagcag atggactggc
     1321 ttggagtcac tgaagtggtg gcgctggtgc tggtcgtggc gctgctcgtg ccgctggtct
     1381 ccctgtacgc acgccgtcgc tggctggccc ggcagggtgg gttgttcgac tgttcactgc
     1441 gcctgtcgac gaagacgccg ggtaccggat gggtgctcgg cgtcgcgcgt tacaccggtg
     1501 acaacctgga gtggtttcga tcgttttcgc tgtcgttccg gccgcgcctg gtgttcccac
     1561 gctcgcagag ccggacggga gcccagcgcg aacccgatcc gatcgaatcg gtgctgctct
     1621 acgacgacca gcggatcgtc aggatggaac tgaccgacgg ctcttcctgg gagttggcga
     1681 tgtcggtagc gtccctgacc ggtctgctca gctggctcga atcgtcccca cccggctccc
     1741 gctaccgctg acgcgagtta ccccgacttc tagggctacc gtcatcgcgg ctccgagagt
     1801 tgacgtcatc ccggctccga gagttgacgt catcccggcg gaggccggga tctcgaactc
     1861 cgcaatcagg agaccgccga cccatcctcg cggcagctag cgggcgcgtc ctcttcgccg
     1921 ccgaacgcgc tatccgtagc gattcgagcg agcaactcgg cgctcagtcc gatccgaggg
     1981 ccgaggtcaa cccatcccgg gttcgactcc gacaccagcc ggttcttcca cgcccggcgc
     2041 cagttcttga gctgcttctc acgtgcgacg gcatcactga tgctgtcccc ggcttcgaag
     2101 tagacgagct tggtgcatcg atagcgcgcg gtgaagccac ccatgcccaa cttgtgttca
     2161 gccacccgcc gaaggaggtc gttggtcacc ccgatgtaga tgacgctgtc acaccgattg
     2221 gtcatgaagt agacgtacgc tccgcgctca cggttccgtg tagttctcat ccgatgaagc
     2281 taggcggtgg ttccgacaat tgtgcgggcg cctgcgctat gacgagatcc cggccttcgc
     2341 cgggatgacg gtgaactccc gagcggacgc cggatgacgg tgaactcccg agcggacgcc
     2401 ggatgacggt gaactcccga gcggacgcct gaccgctgag gtcccaccac cacggcaacg
     2461 cggctgtcgc cctggaggtc gcagcgggcc ttccccaaaa ctcgtatcgc agcctcacct
     2521 catcccggca gtcgttctcc gtcatcccgg cagtcgttct ctgtcatccc ggcgaaggcc
     2581 gggatcttgc gttccaagga ccgacgagcg cttactcggt cttctggatg cggcggcgtt
     2641 gtttggcggc cctggggtcg gtgacctcgc ggtccttgcc agggatccag agcacctcgc
     2701 cggcctgctg gccggctgcg cgggccagga tgaacagcag gtcggacagc cggttcaggt
     2761 aggtgagcgc cagcaggttc accccgcccg gctgatggcc ctcggcgggc tcggtgccgt
     2821 agatctcgac cgcggtccag ccggcgcgct cggcgcgtcg gacgacgctg cgggccaggt
     2881 gcagcagggc cgcggtctgc gtgcctccgg gaaggatgaa cgaacgaagc gccggcagcg
     2941 ggtcgccgaa ctggtcacac catccttcca gccggtcgat gttggcctgg ataacccgca
     3001 gtggctcgta gctggagtgc agcatcagcg ggttggatag gtcggcgccg cagtcgaaga
     3061 gctcattctg gatgtgtcgg aggacggcgc ggaaatcgtc atcgagatcg gattggacga
     3121 cgaccaggcc gagcagggag ttggcctcgt cgacgtgccc gtaggcctcc acccgcgggt
     3181 cggtcttgct ggtctcggac atgtcggaaa gccgtgtccg gccggcgtcc ccggtgcggg
     3241 tgtagatgcg ggtgagattg accatgggtt cactgaacac cgggcacatt tggctgtcaa
     3301 accccgtcga ggcaggatcg ggctcggtcg aagtccgtcc tcggcatgtg gcagcctgta
     3361 ctggtgaaga agctgattct gttgttggtt gcgtccgtac ttgctctcgc tggctgttcg
     3421 tcgggtgccg tccgcggtga gcccgccggc gcgtccagcc cggttgtgtc gtcgagcgcc
     3481 tctgcttctg cgacgactgc tggcgaggtg tcgtgcacct acacggcaac gaatgacggc
     3541 gtgaagaagg tggacctgcc acccacggac ggagtcgcgg ccaccggcac cgccgagtac
     3601 gtgctgaccc tcaactcgga ccctttgaag ctcaccctgg accgcgagaa cgcgccctgc
     3661 acggtgaact ccttctcgtc actggccggt cagggcttct tcgacggctc atcgtgccac
     3721 cggcttgtgg atgacggcgg cctgtacgtg ctgcagtgcg gtgacccgtc cgggaccggg
     3781 tcgggtggcc ccggctacca gttcgacgac gaactcaccg gcagcgagtc ctatggcgct
     3841 ggcaccctgg ccatggcgaa cgcaggcccg ggcacgaacg gctcacagtt cttcatcgtc
     3901 tacgcagaca gccagttgtc gcccgactac acagtgttcg gtcacgtgga cgaggctgcg
     3961 gtgaaggtgc tggcgaagct cgccgcgggc ggccaggaca acagcttcgg cagctccggc
     4021 ggtggcaagc ccaactcgcc cgcagtgatc accagcatca agccggcctg atctgcagcg
     4081 gctggtgggt tgagatcccg gcctacgccg ggatgacgnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnncc tacgccggga tgacgctggc gcagcttcgc caggccacct
     4261 aggcggctca gcggtgcgtt cgggcttcca gctcgagcag cgcggcaacg gtggcgtccg
     4321 ggtcgtcggc atggacccaa tggccggccc cggggaccac cacgcgctcg acgtcaggga
     4381 agagccgggc catgtcggcc tcgtgttccg ctcggacgta cgccgactgc tcgccagtaa
     4441 gccagagcac cggccccggg taggtggcag ggccgggatc aggccagccg ccgatcgcgt
     4501 ccagcgagtc gccgagcagt cgcaggttcg gctgccagtg ccaaccgtgc cggcgacgca
     4561 ggttctgcat caggaacagg cggacggcgt cgtccgggac ggctggactc aggcgggcgt
     4621 cggcttcggc tcgcgaatcg atggtgctca ggtcgaggcc gcgcatcgcg gcaaccagcg
     4681 gggtgaaggt ctccacctgg tccgaatgcc cgggtgcgat gtcgatcacc gccagtccgg
     4741 aagccaactc cggcctgcgc agtgcggtga gcatggcgac cttgccgccc attgaatggc
     4801 cgaccagcac caactgtgcc gccgagccga gccgaaggtc gacctcgtcg gccacggcct
     4861 cggccatccc gaggtagctg aagtggtcgg tccaggcgct ctgcccgtga tcgggcaggt
     4921 cgaagagcac actggccaac tcgtgctcgg ccagccgctg cgcgatctgg ctccaattgc
     4981 ggccacggcc gaagagcccg ggcaggaagg cgaagcgggg acgcccagag ccgacgagtt
     5041 cggtgtgcag ccgtgccatc agcgccgccg ctgccagcat gcggtgtgcc agtgccgtcg
     5101 ttcgtccaca gcggacgtgg agccgatgct cgccagccgg ggccaggcga ccagatggga
     5161 cgctccggga gcgacctgcc ggctgcagcc cgggcaggtg taggtcttgc cggcggcatc
     5221 ggcgggcatg gtctgcacca tccattcacc gtcggccttg atctcgagct gggcgaacgc
     5281 gccagttgac agcggccggg gcgcacgaag gtgcttcgag ggtcgcttgg ccattcagtc
     5341 agcctagcgc cgatgcgggt acgtccggtc aggcgcaccg gccgatcggt gtaggttgac
     5401 cgggtgcgtc tgctgattgc ccgttgccag gttgactacg ccggtcgttt gaccgcccac
     5461 cttccgatgg ccacccgctt gatcatggtg aaagcggacg gatctgtctc ggtccacgcc
     5521 gacgaccgcg cctacaaacc gctgaactgg atgagcgcgc cgtgcacctt gacggtcagc
     5581 ccgccgccgg aggatgtcga ggcggtagcc gaagccgatg aagccgggct caccgaagtg
     5641 tgggaggtgc ggaacacgga cggcgacacc cttcagatcg cgctcggcga ggtgctgcac
     5701 gactcgaacc acgaactcgg tatcgatccg ggattgcaga aggacggcgt tgaagcgcac
     5761 ctacaggttc tgctcgcgga gaatccgcgc acgttcggcg aaggctggac gctgatccag
     5821 cgcgagtact tgacgccgat cggcccggtg gacctactgc tgcgcgacca caccggcgcc
     5881 tatgtcgccg tcgaagtgaa gcgccgtggc gagatcgacg gggttgagca actcacccgc
     5941 tatctggcgt tgatgaacgc ggacccgacc atgagtcccg tccgcggcgt gttcgctgcg
     6001 cagcaaatca agccgcaggc gcggacgctg gccggtaccc gcggcattga atgcgtactc
     6061 gtggactaca acgtactacg agggttggac aacgccgagg atcggctctt ctaatttgta
     6121 gccgtcaggt ccggtccaag tggggcaata gcacctcgcg gtagagcagc agcagggcgg
     6181 ccatcaccgg gatggcgagg atcgcaccga tcattccctg caacaggtag ccggagatcg
     6241 cggccagcat gaccagcacc gacgggacct gcaccgagtg cgaaaagacc cgcggctgga
     6301 tgaagtaggc gtcgaactgc tgataggcga ggaagaagat cagcgtgatc aggccggtgg
     6361 tgggggagaa agagaacgcg atgatcgaca cgatcaccat cgaaatggtg gtaccgacca
     6421 gcggcacgaa ggcgaacagg ccgaccacta cggtcagcgc cagcgcatat tggctcagac
     6481 cgatcacgtt caggaatacg aacgccacac tcatcgcgat cagcaccacg atgaacaggc
     6541 cggacacgta cccgctgacc cgcttgaaca tctcgttggc gaggtacttc acccgcggac
     6601 gccgggacgc gggcgcgagt ttgtagatga cttccttcag attgggcagg gaggtgagga
     6661 agtagagggt gaggaccagg gtgatcacca ccgagaagat ggtgttggcc actgcctggc
     6721 cggcaccgag cagcccgccg aacagactgc tgatcaactc gccggaggtg aggaactcga
     6781 cggccttttc gatcacgtgg aactgggcgt cgaaagcggc gatctgcggg ttctgccgca
     6841 ggttctccag atagtcggga gcttgcagca tcagcttgtt cacctggtcg ctgaccacgg
     6901 gcacgagggc ccaaacggcc agcgagagta gtccgatcgc gctgagcgcg accagcagca
     6961 ctgcgaacgc gcggcggacg ccgtggtggt gcagccagtc caccagcggg ttcagcccga
     7021 gcgcaatcgc caacgaaagc atgagcagca ccagcaccgt gcgcagcgag atcacggtgt
     7081 acaccagtcc ggtggcggcc agcgccccga aagtcaggaa gaacccgatc tggaacgggt
     7141 tgcgatccag cagggagatc cggtcgctgg cgcccagccg ccgaccgtcc gcgtccgccg
     7201 gtgtgggctg ggtggtacgg cgccacttgc gcagccagcc tcgggtcggc ggtgcactgg
     7261 taggcgtatg cgcggatagc gcggccggcg tccgcgcgga cgccggaacc ggcaccccgc
     7321 caggcggtgc cggtggctgc ggttcgctac ccggagtggt cacgagcccg attcaccgtc
     7381 ggtgtagtcg gccacgctca ctcccaccag gttcgccagc tcgccgagct gctcgacgac
     7441 ggactgtcgg cgaccgatga gcagtttcag ctcggcttgc agccgctcca cctccacctc
     7501 ggcttcgcga gtgcgttcag cgcggatgcg ctcgccctcg tgctcggccg cggaggcgat
     7561 gccctcggct tcggctcggg cggacgcgat gatctgctcc gcctcagcga gagcggcggc
     7621 gcggcgctcg ttccacgttt ttgcgtccgc ttcgagccgg gtgcgatagt ccgtgctcac
     7681 ggcctcagcc tcggccagca aatcgtcgag tcgggtcgca ttggctgcct gggcggcctt
     7741 gagttcaccc agatcggctt ctttccgctc ggcgagccgc agcctggctt cggctgcggc
     7801 ccgctccacc tcggcggcgc gggccgccgc gtcgatccgc gcgccctcag cgatcgcagc
     7861 tgcgcgcgcc tgggcgtccg cgaccacttg ggaggcatgg agttccgcgg cctgccggac
     7921 cgcgttggct tcctgcttgg ccgcttccaa ctcggctgca gtctgctcgc tgaggcgctg
     7981 ccggagtgca tccagctcct ggatgctcgc cgcgcgggcg gcgtccaagg cgagctcggc
     8041 ctcaatcgtg aggcgtctcg cctccgcctc cgctgcgctc agcacgagcc gagcgcggct
     8101 gtccgctgag tcgaccaact cgcccgcctg tgactcggcg gccttcaaca tggtgcgggt
     8161 gtgcgcaccg gccttgccga aatcactgtc ggccgcacgg gcgttcgctt cggccagctg
     8221 gcgatgctgt tgccgcaact gcgccttggc ccgcgccaac tggccctcga cgtcgcggac
     8281 gtaggcgtcc acggcgccac gatcgtagcc gcgcaatgac tgcgggaagt tgcccacggc
     8341 cgaggccact tcgtcgaaca gttccagtcc ggtggtgtct tcgcctacca acgtcttccc
     8401 tttcgctcgc ccaatgatgc tagtggcccg gcccgacaaa tcgggccggg ccagtgcagg
     8461 tcagtgaatc agtgggccgc agcagccggc tcgacgatct cgagcaggat gccaccgaca
     8521 tccttcgggt gagcgaagtt gatccgggaa ccgccggtgc cgatcttggg cgcgtcgtag
     8581 agcaggcgaa cgccacgctc gcgcagcacc gcactgacct tgtcgaggtc ggccacccgg
     8641 taggcaacct gctggattcc gcccttgccg ccgttgcgct cgatccactt cgcgatcgtg
     8701 ctgttctcgt tcagcggtgc cagcaactgc acctgggcgt tctcggtgag ctggtcaccg
     8761 gtaccgatca tcgcctcctc gacgccctgc tcagcgttgg tctcgcggtg cagcacccgc
     8821 cagccaagca cttcggtgtg gaacttgatg gcgtcgtcaa gattgtggac ggcgtagccg
     8881 acatggtcga tgcagatgaa aaggtcgtcg ttgctcatgt tgtccagcct attcccaatg
     8941 ctcacgacag aacgccgtcg accacttcct tggcctcggc ctggacctgt gccaggtgtt
     9001 ccgggccgcg caacgattcg gcgtagatct tgtacacgtt ctcggttccg gacgggcgcg
     9061 ccgcgaacca ggcggacgcg gtggcgacct tgagcccgcc gatggccgcg ccgttgcccg
     9121 gggcatgggt aagcgtggcc acgatcggtt cgccggccag ttcggttgcg gtgacgtccg
     9181 acgcgctcag cttggcgagc ttcgccttct gctcgcggct ggcgggtgca tccacccggg
     9241 cgtagctgga cgcaccgtgg cgggcctcca gttcggcgta cagctcggac ggcgacttgc
     9301 ccgtgaccgc cttgatctcg ctggccagca gagcgagcag gatgccgtcc ttgtcggttg
     9361 tccacgtgga gccatccctg gcaaggaaag atgcacccgc tgactcttcg ccgccgaacc
     9421 cgagatcacc gctcatcagg ccgggcacga accacttgaa gcccaccggc acctcgacca
     9481 accgacggcc tagatcggca gccaccttgt cgatcagcga actcgacacc agggtcttgc
     9541 ccaccccggc gtccggacgc cagttggggc gatgaccgaa caggtactga atcgccaccg
     9601 ccaggtaggc gttgggattc atcagaccgt agtcgggggt gacaatgccg tgccggtccg
     9661 cgtccgcgtc attgccggtg gagatctcgt aggactcccg gttcgcgatc agggaggcca
     9721 tggcgtaggg cgaggagcag tccatgcgga tcttgccgtc ccagtccagc gtcatgaacg
     9781 gccaggccgg gtcgacctgc gggttgatca cggtcaggtc gatgcccagc cgctcggcga
     9841 tgtagcccca gtactgcacg gacgccccac cgagcggatc ggcgccgatc cgcacacccg
     9901 cggacgcgat cgcgtccagg tcgagcgcgt tgcgcaggtc ttcgcagtaa gcacctaagt
     9961 agtccacgcg ggtgacgtcg cgcgcggcgg tcgcgtacgg cgtccgcttg atcgaaccga
    10021 ccgagcgcag cagctcgttg gcccgggcgg cgatcaccga ggtggcgtcg gtgtcggccg
    10081 ggccgccgtg cggcgggttg tacttgaa
//
LOCUS       DAOPRQ010000084         9665 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_5916_length_9665_cov_12.003746, whole genome shotgun sequence.
ACCESSION   DAOPRQ010000084 DAOPRQ010000000
VERSION     DAOPRQ010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 9665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9665
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5916_length_9665_cov_12.003746"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="PKJ61_10205"
     CDS             complement(<1..206)
                     /locus_tag="PKJ61_10205"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007400908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HOC14234.1"
                     /translation="MTINLGMPAVPPPVLAPRRPTRQIKVGKVLVGGGAPVSVQSMTT
                     TKTTDINGTLQQIAELTATGCDIVR"
     gene            complement(301..594)
                     /locus_tag="PKJ61_10210"
     CDS             complement(301..594)
                     /locus_tag="PKJ61_10210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14235.1"
                     /translation="MSQATFLATTAAGLRAGVPSTALPGLLDQHHHSRRLHQLGVSPA
                     PLTLARVNPVRLAGPLRELTGGDYADRPVAVGESVRALDGAELTASILAGLVE"
     gene            complement(600..1895)
                     /locus_tag="PKJ61_10215"
     CDS             complement(600..1895)
                     /locus_tag="PKJ61_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HOC14236.1"
                     /translation="METSTVIGFAIAFFTLIMASVALHELGHMIPAKRFGVRVPKYFV
                     GFGPTLWSTTRGETEYGIKWFPLGGFVRLLGMYPPPPLDDRPRAASRLNQLADAARDQ
                     EWQEITPTDVTDKRLFYQKKTWQKLVVMAGGPVMNLLIAFVLMLGVTGLYGVYRAQTT
                     VGYVQPCVLTDSTRTECADTDPKSPAAAAGLQAGDRVVSFNETMITSYSQLSSLIRAN
                     LDAEARLVVERGGQEVALAPVHTVINEVADTLDPGRRLSAGWLGVSPKQELVKGGPVE
                     VLGDMWLQTKQSVVALVQFPVKVWHVMVGMVTGQPRDVYDPISILGASTVAGQVIASD
                     QVTPGAKVATFVSLLASVNLFLALFNFVPLPPLDGGHIAGALYEWVRRKFATLSKRPD
                     PGFFDTAKLLPVAYGVGGLLLLSGVVLILADIISPISLF"
     gene            complement(1899..3053)
                     /gene="dxr"
                     /locus_tag="PKJ61_10220"
     CDS             complement(1899..3053)
                     /gene="dxr"
                     /locus_tag="PKJ61_10220"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015582431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HOC14237.1"
                     /translation="MRQVVVLGSTGSIGTQAIEVITARPEQFEVVGLAAGGSSIELLV
                     EQARTLRPQVVAVSSPEAADRLAAALPGQEVWAGPEAATRLAAISADVVLNGITGAAG
                     LEPTLATLNAGTTLALANKESLVIGGRLVTDAAAPGQIVAVDSEHSAFAQALRAGRAE
                     EVRKLVLTASGGPFRGRTRAELRQVTPAQAMAHPTWDMGRVITINSATLVNKGLELLE
                     AGLLYDVDLDAIEVVVHPQSIVHSMVEFVDGSTIAQCSPPDMRLPIALGLTWPDRLAN
                     AAAPCDWKKAATWTFEPLDSAAFPAVELAREAGKAPGGLAPAVYNAANEVCVDAFCAG
                     RLEFPGIVDVVADVVATHLARHAAEPLSLAAVLAADQWARQAAAERISKE"
     gene            3165..4253
                     /locus_tag="PKJ61_10225"
     CDS             3165..4253
                     /locus_tag="PKJ61_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012922306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HOC14238.1"
                     /translation="MTTAPAGVEIESLAQFDARLAAAGNLVNCYLQSLDLSGRSAALG
                     TVDVTGAVFLGCVLEPGIEPELERRGALLFPTLPDLPFNPYRSGLYSASELYAGLERG
                     YRYTLDARVFRWWRYVGQHRSLTSELAMTLHDHAIVEALEDLRLEGAVGVMGGHQLVR
                     GSADYSDAVHLGRSLAATGRTVITGGGPGAMEAVNLGAALIGTRSELEAAIALLAGRP
                     RFAEDVASWVAAGLAVRERWELSGASYGVPTWLYGHEPPNVFSAGVAKLFSNAIREDM
                     LLRQASGGLVCLPGAAGTIQEIFQAATPRYYAAAGEPIPPLVLVGVDYWTRERPAWPL
                     LANLADGRPMSAAIHLVDDIAEVSGLLG"
     gene            complement(4432..5658)
                     /gene="rlmN"
                     /locus_tag="PKJ61_10230"
     CDS             complement(4432..5658)
                     /gene="rlmN"
                     /locus_tag="PKJ61_10230"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOC14239.1"
                     /translation="MTGLTPEEVADYQRSIAHAESGRTVPLLLNTPRRGKPPVHWADL
                     DATQRAEAIGELGLPKFRAAQLTKHLFERLATSPDDWTDIPKSARTQLAERFVPDLAE
                     RVRDQQADGGTTVKTLWRLHDGSLVESVLMRYGVRGIRASGKGRERATVCISSQAGCG
                     MACPFCATGQGGLQRNLSTAEILTQVMDAARRLRDGEIPGGPGRVNNIVFMGMGEPMA
                     NFNAVLRAVRTITAPAPDGLGISARGVTVSTVGLVPQIEKLAAVGLPVTLAVSLHAPD
                     DRLRDELCPINTRWQVAEVLAAARNYFATTGRRVSIEYALIRDINDQAWRAEALADVL
                     RGASAEGRNGDYGWVHVNLIPLNPTPGSKWTASRREDEAEFVHRLEARGVPVTVRDTR
                     GREIDGACGQLAAGQA"
     gene            complement(5655..6527)
                     /locus_tag="PKJ61_10235"
     CDS             complement(5655..6527)
                     /locus_tag="PKJ61_10235"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002527010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOC14240.1"
                     /translation="MTLSEPKSPPKSRAGRDLPAAIAVGLLLFGIVVVTLMWWPPGFI
                     ILLVVLTSLGAVELHQALMKIGMRSAIVPVVIGNILIIGGSYLAATLQNVTNVPWHVV
                     LLGTIGGTILLSLIWRMFGGADGYVRDSAAGLFIIGYLPVMASFVGLLLAQQHGVAKV
                     VAAFLCIVGSDTGGYAAGASLGKHPMAPNISPKKTWEGLVGSFALAGVLGIGMVTLVM
                     HQPWWIGAILSVVVVVFGTAGDLIESLIKRDIGIKDMSSFLPGHGGVMDRLDSTLIAV
                     PAAWLLFTLVGVGA"
     gene            complement(6528..7082)
                     /gene="frr"
                     /locus_tag="PKJ61_10240"
     CDS             complement(6528..7082)
                     /gene="frr"
                     /locus_tag="PKJ61_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002516897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOC14241.1"
                     /translation="MPEILKDADHKMNGAVEHAREEFAAIRTGRAHPAMFNKLMVDYY
                     GSPTPLQQLATFQVPEARTVLISPFDRGAMAGIEKAIRDSDLGVNPANDGNVIRLVMP
                     QLTEERRKEYIKVTRHKAEEARVAVRNIRRHSIEHVGKLTKDKEISEDDARGAEKQLD
                     AATKKHIDTIDELLKAKEAELLEV"
     gene            complement(7116..7829)
                     /gene="pyrH"
                     /locus_tag="PKJ61_10245"
     CDS             complement(7116..7829)
                     /gene="pyrH"
                     /locus_tag="PKJ61_10245"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002548354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOC14242.1"
                     /translation="MTSPYRRVLLKLSGEAFGGGKLGVDPDIISSIAGQIAAVVASGV
                     QVAIVTGGGNFFRGAELQTRGMDRSRADYMGMLGTVMNCLALQDFLEKVGVPTRVQTA
                     ITMGQVAEPYIPRRAIRHLEKDRVVIFGAGSGMPYFSTDTVAAQRALEIGAEVLLMAK
                     QGTDGVYDADPRTNPHAKKFDELTYDDFLARDLKVADATAVSLAREHRLPMVFFDLGA
                     EGSIAKVVAGEKIGTTLHA"
     gene            complement(7974..8789)
                     /gene="tsf"
                     /locus_tag="PKJ61_10250"
     CDS             complement(7974..8789)
                     /gene="tsf"
                     /locus_tag="PKJ61_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012922315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOC14243.1"
                     /translation="MATITAADVKKLRDATGAGMMDAKKALTEADGDFDGAVEILRVT
                     GQAKMAKRSDREASNGLVAASGKTVIQLGAETDFVAKNAEFMALAEAIAAAVDAAGAH
                     GVEAAGAVALPSGQTVAEAVAELSAKIGEKLELAAAATFDGDVSIYLHKRSQDLPPQV
                     GVMVEHAGGDEAFVRGIAMQIAAMSPAYVTRDEVPAEVLDNERKIAELTAKEEGKPEQ
                     IIPRIVEGRLGGFYKEVCLVEQASVSDDKLTVGQLLKNNGVEVKRFVRFAAGA"
     gene            complement(8807..>9665)
                     /gene="rpsB"
                     /locus_tag="PKJ61_10255"
     CDS             complement(8807..>9665)
                     /gene="rpsB"
                     /locus_tag="PKJ61_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007077458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOC14244.1"
                     /translation="FIFTERNGIYIIDLQQSLKYIDNAYAFVKATVARGGQIMFVGTK
                     KQAQEAIAEQATRVGMPYVNQRWLGGMLTNFGTISKRIAKMKELEGLNFDDVAASGLT
                     KKELLQRKRKMEKLAKTLGGIRDMHKLPQAIWVVDTNKEHLAIDEARKLRIPIVGILD
                     TNCDPDQVDFPIPGNDDAIRSVALLTRVLADAVAEGLLARSAGTTTGEEVEPMPDWER
                     DLLAGAPAEAEAVAVEVAEPAAEVAEPVADVEVAAEAVAEPVAEVVAETTEAAAAEVV
                     AELAADETK"
BASE COUNT         1484 a         3305 c         3273 g         1603 t
ORIGIN      
        1 cggacgatgt cacaccccgt ggcggtgagt tccgcgatct gctggagggt gccgttgatg
       61 tcggtggtct tggtcgtggt catcgactgc accgagaccg gcgcaccgcc acccaccagc
      121 accttgccaa ccttgatctg gcgggtcgga cgacgcggcg ccagcaccgg cggcggaact
      181 gcgggcattc cgaggttgat ggtcatcggc tcctcaatca cgacgaacgg gcggcggctc
      241 gcggccaccg tcgacagcct acccatccga gctgcggacg cgggaaacgg cgtcgaacgc
      301 ctactccacc agcccggcca ggatcgaggc ggtcagctcg gctccgtcca gtgctcgtac
      361 cgactccccc accgctactg gccgatcggc gtagtcgccg ccagtcagct cccgcagcgg
      421 accggccagg cgcactgggt tgacccgggc cagcgttagg ggtgccgggc tcacgcccaa
      481 ttggtgcagg cggcgggagt ggtggtgctg gtcgagcagg ccgggaaggg cggtcgaggg
      541 cacgccggca cgcaacccag cggcagtggt ggccaggaag gtcgcttgcg acatccggct
      601 cagaagaggc tgatcggcga gatgatgtcg gccaggatca gcaccacgcc gctgagcagc
      661 agcagtccac ccaccccgta ggcgaccggg agcagcttcg ccgtgtcgaa gaagccggga
      721 tcaggacgct tgctgagcgt ggcgaacttc cgccgcaccc actcgtacag ggccccggcg
      781 atatggccgc cgtccagcgg gggcagcggc acgaagttga acaacgcaag aaacaggttg
      841 accgaggcca gcagcgagac gaaggtggcc actttcgccc ccggggttac ctggtcggac
      901 gcgatcacct gtccggcgac agtgctggcg ccgaggatcg agatcgggtc gtagacgtcc
      961 cgcggctgac cggtcaccat gccgaccatc acgtgccaca ccttcaccgg gaactgcacc
     1021 agggcgacga cgctctgctt ggtctgcagc cacatgtcgc ccagaacttc caccgggcca
     1081 cccttgacga gttcctgctt tggtgaaacc cctagccaac ctgcactcag ccggcggccc
     1141 gggtcgagcg tgtcggccac ctcgttgatc acggtgtgca ccggggccag cgctacctcc
     1201 tggcctccgc gctcgacgac cagccgggcc tcggcgtcaa ggttggcgcg gatcagcgaa
     1261 ctcagctggc tgtacgaggt gatcatggtc tcgttgaagc tgaccacgcg gtccccggcc
     1321 tgcagcccag cagcggcggc cggcgacttc ggatcggtgt cggcacactc ggtgcgggtg
     1381 ctgtcggtga gcacgcacgg ctgcacgtag ccgaccgtcg tctgcgcccg gtagaccccg
     1441 tagagcccgg tcacgccgag catcaggacg aaggcaatca gcaggttcat caccgggccg
     1501 ccggccatca cgaccagttt ctgccacgtc ttcttctggt agaacagccg cttgtccgtg
     1561 acgtcggtcg gcgtgatctc ctgccactcc tggtcccgag cggcgtcggc cagctggttc
     1621 agccgcgagg ccgcacgggg acggtcgtcg agcggtggcg gcgggtacat gcccagcagc
     1681 cggacgaagc cacccagcgg aaaccacttg atgccgtatt ccgtctctcc tcgggtggtg
     1741 gaccacagcg tcgggccgaa acctacgaag tacttgggca cccgcacacc gaagcgcttg
     1801 gccgggatca tgtggccgag ttcgtgcagc gcaaccgacg ccatgatcag cgtgaagaag
     1861 gcgatcgcga agccaatgac ggtgctggtc tccatgcgct actccttgct gatccgctcg
     1921 gctgccgcct gccgcgccca ctggtcggcc gccagcacgg cggccaggct caggggctcg
     1981 gcggcgtgcc gggccagatg ggtggccacc acgtcggcga ccacgtccac gatgccaggg
     2041 aactccaacc gtccggcaca gaaagcgtcc acgcagactt cattggccgc gttgtagacg
     2101 gctggcgcga gcccaccggg ggccttgcca gcttcccggg ccagttccac ggcagggaat
     2161 gccgcggagt cgagcggttc gaaggtccag gtggccgcct tcttccagtc gcagggggca
     2221 gccgcgttgg cgagacggtc gggccaggtg agcccgagcg cgatcggcag gcgcatgtcc
     2281 ggcggcgagc actgcgcgat cgtcgaaccg tccacaaact cgaccatcga gtgcacgatg
     2341 gattgcgggt gcacgaccac ctcgatcgcg tccaggtcga cgtcgtagag caggccggct
     2401 tccagcagtt cgagtccctt gttcaccagg gtggcggagt tgatcgtgat cacgcggccc
     2461 atgtcccagg tggggtgcgc catggcctgg gccggggtga cctgccgcag ctcagctcgg
     2521 gtgcgaccac ggaaagggcc gccggacgcg gtcaggacga gtttgcgcac ctcttccgcc
     2581 cggcccgctc gcagtgcttg ggcgaaggcg gaatgctcgg agtcgaccgc tacgatctgg
     2641 ccgggtgcgg cggcgtcggt gaccagtcgg ccgccgatca ccagcgactc cttgttggcc
     2701 agggcgagcg tcgtacccgc attgagcgta gccagcgtgg gttcgaggcc ggcggcgccg
     2761 gtgatgccgt tgagcaccac atccgccgag atcgccgcga gccgcgtggc agcttccggt
     2821 cccgcccaga cttcctggcc gggcagggcc gccgccagcc ggtcggcggc ctcgggcgaa
     2881 ctgaccgcaa ccacctgcgg acgcagcgtc cgagcctgtt cgaccagcag ctcgatggat
     2941 gagcccccag ctgccaggcc gaccacttcg aactgctccg ggcgcgccgt gatcacctcg
     3001 atggcttggg tgccgatcga acctgtacta ccgagtacta caacctggcg catcggccaa
     3061 tcacctccca cacggaaccc ctgcagccta tcggccgcca ccgacacggc gtcccggtgc
     3121 gttgagtagg gtcggagcga atcgagtttg atgggagcgg ccaggtgacc acagcgcccg
     3181 ccggggttga gatcgaatcc ctggctcaat tcgacgccag gctcgcggcc gccggcaacc
     3241 tggtgaactg ctatctgcag tcgctcgact tgagcggacg ctcggccgcg ctcggcacag
     3301 tcgacgtgac cggcgcggtc tttctcggct gtgtactcga acccggcatc gagccggagt
     3361 tggagcggcg cggcgcactg ctcttcccca ccctgcctga cctgccgttc aacccgtacc
     3421 gctcggggct gtacagcgcg agcgaactct atgcggggct ggagcgtggc taccgctaca
     3481 ccctggacgc aagggtgttc cgttggtggc gttacgtcgg ccagcaccgc agcctgacca
     3541 gcgaactcgc catgaccctg cacgaccacg ccatcgtcga ggcgctggag gatctgcgcc
     3601 tcgaaggtgc ggtgggcgtg atgggtggcc accagttggt gcgcgggtcg gccgactatt
     3661 ccgacgccgt gcaccttggc cgctcgctgg ccgcaaccgg acggacggtg atcaccggcg
     3721 gtggaccggg cgcgatggag gcggtgaatc tcggcgccgc cctgatcggc acccgcagcg
     3781 agttggaggc tgcgatcgca ctgctggccg ggcgcccccg gttcgccgag gacgtggcgt
     3841 cctgggtggc cgccgggctg gccgtgcgcg agcgttggga gttgagcggc gccagctacg
     3901 gcgtgccgac ctggttgtac ggccacgagc cgccgaacgt gttcagcgcc ggggtggcga
     3961 agctgttctc gaacgcgatc cgcgaggaca tgctgcttcg ccaggcatcc ggcgggttgg
     4021 tctgccttcc cggtgccgca ggcacgatcc aggagatctt ccaggctgcg acgccgcgct
     4081 actacgccgc tgccggcgag ccgatcccgc cactggtgct ggtcggcgtc gactactgga
     4141 cccgggagcg tccggcctgg ccgctgctcg ccaacttggc ggacgggcgt ccgatgagcg
     4201 cggcgatcca cctggtcgac gacatcgccg aggtgtcggg gctgctcggc tgaactcgga
     4261 ggcaccaggc tcgcccgaca gccctagtcc gccgagtcgt gaacttcgct cccgaaaggg
     4321 actcgcgctc ccgaattatc gggagcaacg gtccccttcg ggagcgaagt tggaggtcgg
     4381 tttggtgcta gaccagcggt tgccacgacc cttcgtcaag ctcagggatc gtcaggcttg
     4441 gccggcggcg agctggccgc aggcgccgtc gatctcgcgg ccgcgggtgt cgcggacggt
     4501 gaccggcact cccctggctt ccaggcggtg gacgaattcg gcctcgtcct cacgacgcga
     4561 tgcggtccac ttcgacccgg gcgtcgggtt gagcgggatc aggttcacgt gcacccagcc
     4621 gtagtcgccg ttgcgaccct cagcggacgc gccgcgcagc acgtcggcca gcgcctcggc
     4681 ccgccaggcc tggtcgttga tgtcacggat cagggcgtac tcgatcgaga cgcggcgtcc
     4741 ggtggtcgcg aagtagttcc gcgcggcagc cagcacctcg gccacctgcc aacgggtgtt
     4801 gatcgggcag agttcatcgc gcagccggtc gtccggcgcg tgcaggctca ctgcgagcgt
     4861 taccggcaac ccgaccgcgg ccagcttctc gatctgcggc accaagccga ccgtcgagac
     4921 ggtcacaccc cgcgccgaga tccccagacc gtccggcgcc ggcgcggtga tcgtacgcac
     4981 tgctcgcagc acggcgttga agttcgccat cggctcgccc atgcccatga acacgatgtt
     5041 gttcacccgg cccggaccgc cgggaatctc gccgtcgcgc aaccggcggg cggcgtccat
     5101 cacctgggtg aggatctccg cggtgctcag attgcgctgc agcccgccct gaccagtggc
     5161 gcagaacggg caggccatgc cgcagccggc ctgggaagag atacagacag tggcgcgctc
     5221 gcgtcccttg ccgctggccc gaatcccgcg gacgccgtag cgcatcagca cgctctccac
     5281 cagcgagccg tcgtgcagcc gccaaagggt cttcaccgtg gtgccaccgt cggcctgctg
     5341 gtcgcggacg cgttcggcca ggtccggcac gaagcgctcg gccaactgcg tccgggccga
     5401 cttcgggatg tccgtccaat cgtccggcga ggtggccagc cgctcgaaca ggtgcttggt
     5461 cagctgtgcc gcccggaact tcggcaggcc gagttcccca atcgcttccg cgcgctgggt
     5521 cgcgtccagg tcggcccaat ggacgggtgg cttgccccgg cgcggcgtgt tcagcagcag
     5581 cggcaccgtg cgtccggatt cggcgtgcgc gatcgaacgc tggtagtcgg cgacctcctc
     5641 aggcgtcaac ccggtcatgc gcccaccccc accagggtga acagcagcca ggccgcaggc
     5701 accgcgatca gggtcgagtc gagccggtcc atgacgccgc cgtggccggg caggaaggag
     5761 ctcatgtcct tgattccgat gtcgcgcttg atcagcgact cgatgaggtc accggcggtc
     5821 ccgaaaacaa ccacgaccac cgagagaatc gcgccgatcc accacggctg gtgcatgacc
     5881 agcgtgacca tgccgatccc cagtacgcca gccagggcga acgagccgac caggccctcc
     5941 caggtcttct tggggctgat gttcggcgcc atcgggtgct tgccgagcga cgcgccggca
     6001 gcgtacccgc cggtgtcact gcccacgatg cagaggaacg cagccaccac cttggccacg
     6061 ccgtgctgct gagccagcaa caacccgacg aacgatgcca tcaccgggag gtagccgatg
     6121 atgaacaggc ccgccgcgct gtcacgcacg tacccgtccg cgccgccgaa catccgccag
     6181 atcagcgaca gcaggatcgt gccgccgatc gtgccgagca acaccacgtg ccagggaaca
     6241 ttggtcacgt tctgcagggt cgcggcgagg tacgagccac cgatgatgag gatgttgccg
     6301 atcaccaccg gcacgatcgc cgaacgcatg ccgattttca tgagcgcctg gtgcaattcc
     6361 accgcaccga ggctggtcag caccaccaga aggatgatga acccgggcgg ccaccacatc
     6421 agggtgacca ccacgatgcc gaagagcagc agcccgaccg cgatcgcggc gggcagatcc
     6481 cgcccggcgc gcgatttcgg tgggctcttc ggctcgctca aggtcactca gacctccagc
     6541 agttcggctt ccttggcctt cagcaactca tcgatcgtgt cgatgtgctt cttggtggcc
     6601 gcgtccagct gcttctccgc accccgagcg tcgtcctcgc tgatttcctt gtccttggtc
     6661 agcttgccga cgtgctcgat cgaatgccgc cggatgttgc ggacggcaac ccgcgcctcc
     6721 tcggccttgt ggcgagtgac cttgatgtac tccttgcggc gctcctcggt gagctgcggc
     6781 atcaccagcc ggatcacgtt gccgtcattg gccgggttca cgcccaggtc ggaatcacgg
     6841 atcgccttct cgatgccggc catcgcgcca cgatcgaacg ggctgatcag cacggtgcgg
     6901 gcctccggca cctggaaggt ggccagttgc tgcagcggtg tcggtgagcc gtagtagtcc
     6961 accatcagct tgttgaacat agcggggtgg gcgcgtccgg tgcgaatggc cgcgaactcc
     7021 tcgcgcgcat gctccaccgc accgttcatc ttgtggtcgg cgtccttgag gatctccggg
     7081 atcacgaggt ctttcccttc tgcttcgcct gcacgtcacg cgtgcagggt ggttccgatc
     7141 ttctcacctg cgaccacctt ggcaatgctt ccctcggcac ccaggtcgaa aaagaccatc
     7201 gggagccggt gttcgcgggc caggctgacc gcggtcgcgt ccgccacctt caggtcgcgg
     7261 gcaaggaagt cgtcgtaggt gagctcgtcg aacttcttcg cgtggggatt ggtgcggggg
     7321 tcggcgtcgt agacaccgtc ggtgccctgc ttggccatca gcagtacctc agcgccgatc
     7381 tccagcgccc gctgggctgc gacggtgtcg gtggagaagt acggcatgcc cgagccggcg
     7441 ccgaagatga ccacgcggtc cttctccaga tgccggatcg cgcgtcgcgg gatgtagggc
     7501 tcggccacct ggcccatggt gatcgctgtc tggacgcggg tgggcacccc gaccttctcc
     7561 aggaagtcct gcagcgcgag gcagttcatc acggtgccga gcatgcccat gtagtcggcc
     7621 cgggagcggt ccatgccgcg agtctgcagc tcggcaccgc ggaagaagtt gccgccgccg
     7681 gtgacgatcg cgacctgcac cccggacgcg accactgccg cgatctggcc ggcgatgctg
     7741 gagatgatgt ccggatcgac gccgagcttc ccgccgccga aggcttcacc cgagagtttc
     7801 agtagtacgc ggcggtaggg gctggtcatt tgtctcctag gacgggcggt gaaagcgtcc
     7861 ctaccctacc gaaactggcg ggccggttcg gactacgggt gcgcccgagt tccctggaaa
     7921 caccaggacg gccgcccagc atcgctgggc ggccgtcctg gctgcaactg ggatcaggct
     7981 ccggcggcga agcggacgaa ccgcttgacc tcgacgccgt tgttcttcag cagctggccc
     8041 acggtgagct tgtcgtcgga gaccgacgcc tgctcgacga ggcatacctc cttgtagaag
     8101 ccgcccaggc gaccctcgac gatgcgcggg atgatctgct cgggcttgcc ctcctccttg
     8161 gcggtcagct cggcgatctt gcgctcgttg tcgagcacct cggccggcac ctcgtcacgg
     8221 gtgacgtacg ccggggacat cgccgcgatc tgcatggcga tgccacgcac gaacgcttcg
     8281 tcgccaccgg cgtgctccac catgacgccc acctgcgggg gcaggtcctg cgaccgcttg
     8341 tgcaggtaga tgctgacgtc gccgtcgaag gtagcggctg cggccagttc gagcttctcg
     8401 ccgatcttgg cgctgagctc tgcgaccgct tcggccacgg tttgaccgga cgggagcgcg
     8461 accgcgccgg ccgcctcgac gccgtgcgcg ccagcggcgt ccacggcggc ggcgatggcc
     8521 tcggccagcg ccatgaactc agcgttcttc gcgacgaagt cggtctcggc acccagctgg
     8581 atgacggtct tgccggacgc ggcgaccagg ccgttgctgg cctcacggtc ggagcgcttg
     8641 gccatcttcg cctggccggt gacccgcagg atctccacgg cgccgtcgaa gtcgccgtca
     8701 gcctcggtga gggccttctt cgcgtccatc atgccggcgc cggtggcgtc gcggagcttc
     8761 ttgacgtccg cggcagtgat cgttgccata tctgttctct cgttccttac ttggtctcgt
     8821 cggcggcgag ctcggccacg acctcggcgg cggcggcttc ggtggtctcg gcgaccactt
     8881 cggcgaccgg ctcggccacg gcttcagcgg caacctcgac gtcggctacg ggctcagcaa
     8941 cctcagcggc cggctcggcg acctcgacag ccacggcctc ggcctcggcc ggagcgccgg
     9001 ccagcaggtc gcgctcccaa tcgggcatcg gctcgacctc ttcgccggtg gtggtgccgg
     9061 cggagcgggc cagcaggccc tcggccaccg cgtcagcgag cacgcgggtg agcagcgcga
     9121 ccgagcggat cgcgtcgtcg ttgcccggga tcgggaagtc gacctggtcg gggtcgcagt
     9181 tggtgtccag gatgccgacg atcgggatgc gcagcttgcg cgcctcgtcg atagccaggt
     9241 gctccttgtt ggtgtcgacc acccagatgg cctgcggcag cttgtgcatg tcgcggatgc
     9301 cgccgagggt cttggcgagc ttctccatct tgcgcttgcg ctgcaggagt tccttcttgg
     9361 tcaggccgga cgccgccacg tcgtcgaagt tcaggccctc aagctccttc atcttcgcga
     9421 tgcgcttgga gatggtgccg aagttggtga gcatgccgcc cagccagcgc tggttcacgt
     9481 agggcatgcc gacgcgggtg gcctgctcgg cgatggcctc ctgggcctgc ttcttggtgc
     9541 cgacgaacat gatctggccg ccgcgggcga cggtcgcctt cacgaaggcg taggcgttgt
     9601 cgatgtactt cagcgactgc tgcaggtcga tgatgtagat gccgttgcgc tcggtgaaga
     9661 tgaac
//
LOCUS       DAOPRQ010000085         9397 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_6126_length_9397_cov_12.952259, whole genome shotgun sequence.
ACCESSION   DAOPRQ010000085 DAOPRQ010000000
VERSION     DAOPRQ010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 9397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9397
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6126_length_9397_cov_12.952259"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            455..2293
                     /locus_tag="PKJ61_10260"
     CDS             455..2293
                     /locus_tag="PKJ61_10260"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009213061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HOC14245.1"
                     /translation="MPVDTSTITTNVPENAPEALVTWVNGVADLTQPDAVYWCDGSVD
                     ERDRLYAEMVAAGTFTKLNQELRPGCYLARSTPSDVARVESRTFICSEREEDAGPTNN
                     WADPVEMKQILATKFAGSMRGRTMYVIPFSMGPLGGDISKLGIEVTDSPYVVVNMRIM
                     TRIGTPALDHIAAGEPWVPAVHSVGYPLVDADGNPRDDVAWPCNDDKYITHFPETHEI
                     WSYGSGYGGNALLGKKCYALRIASVLAREQGWLAEHMLILKITGPDGREYHLTAAFPS
                     ACGKTNLAMLRPTIPGWKVETVGDDIAWMRPGKDGRLYAINPEAGFFGVAPGTSLDTN
                     PTCLNALDHDVIFTNVALTDDGDIWWEGLTKQPPAHLIDWQGNDWTPDSGRPAAHPNS
                     RFTVHASQAESISPDWEDPAGVPIDVILFGGRRATNVPLISESYDWNHGVFVGATVSS
                     EQTAAAEGAVGSLRRDPFAMLPFCGYNMADYWAHWLEIGEVLGDKAPRIFQVNWFRKD
                     SDGKWLWPGFGDNSRPIEWALRRVNGEVGAVDAISGRIPAPGDLNVSGLDITDAELTA
                     LFELDPDAWAKEADLTEEYFAQFGDKLPAELTSQLAGLRERIAAAK"
     gene            complement(2528..3541)
                     /locus_tag="PKJ61_10265"
     CDS             complement(2528..3541)
                     /locus_tag="PKJ61_10265"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOC14246.1"
                     /translation="MAKRATLADVARLAGLSPTTVSMVLNNRPNTRLSEEAAERVRAA
                     AARLNYRPNPAARGLRLGKTRTVGFISDEVTITRYASALIRGLLEEAEKREHTVLISE
                     SGRKISRMAEALDAMLDRQTDGIVFGLQAAKQIQVPKLSVEVPVVMANATSTLGHPCV
                     LPDEFQAGYDAVRYLTDRGHRRLALIGRSQVLMAAANSATVAQRYAGMDAALAEDGIE
                     FLHEVTAGKDWEPELGYRGAHEILGKVSVTAMVAANDRVAFGVYQAVQERGLRVPDDI
                     SVISFDDEYLASYLRPGLTTMHIPYLEMGQTAMRLVLDGDAPAQTLIPMPVRERGSVR
                     TLG"
     gene            complement(3640..4728)
                     /locus_tag="PKJ61_10270"
     CDS             complement(3640..4728)
                     /locus_tag="PKJ61_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015582009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HOC14247.1"
                     /translation="MSSLFNALQLRDVTIPNRIFLAPMCQYQAVDGVPNDWHVVHYGA
                     RAAGGFGLIIAEATGVSPEGRITPYCCGLWNEEQTNAWRRIVEFGHSQGAKMAIQLQH
                     AGRKASVYREFEPGKSGSVPVEEGGWETIGPSAIPFPGLATPREATGSDLRKVVADFA
                     AAAQRADAAGFDAVEIHAAHGYLIFQFLSPLSNHRTDEYGGDLAGRSRLLLEVADAIR
                     AVWPAGKPLLVRISATEWVEGGFTVEEATEVARMLADHGVDLVDVSSGGNVLAKINAL
                     PGYQVPLAAAVREAGLPVSAVGWITEPAQAQAILDDGLADVISLGRVALREPSWPLRA
                     AAELDADHMARYPDSYLRGRWPAVEPVR"
     gene            complement(4915..7101)
                     /locus_tag="PKJ61_10275"
     CDS             complement(4915..7101)
                     /locus_tag="PKJ61_10275"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HOC14248.1"
                     /translation="MLALTDVQVRAGLASAQWIAPDKAPGEPGKRPAYLLRACFDASD
                     VAKSLLVHATAHGLYEAFLNGSRIGDMELAPGFSAYRKRLEVQSCDVSELVVPGPNTL
                     VVVLSDGWYRGRHGFERRADGFGDQTALLLALTDDAGEPILATGPRWESGPSHILRAD
                     LMDGQTTDLRLLDPDWFTGADGPGWQQVVVPEGPIYADRSRLVAPIAPPVRRTQELAP
                     VAVTRPRPGTVVLDFGQAINGWVRLTDLGPAGTRLVLTHGEALAVSGLVNTDHLRAFV
                     FATGQVLPAGQVDEVIASGLVGESFEPRHTTHGFRFVQVDGVPDDLDLSSARGVVVHS
                     DLRRTGEFGCSDARLARLHEVVRWSLRNNVCAIPTDCPQRERSGFTGDWQLFVSTAAM
                     MADVQRFSRRWLRDLAADQWADGRIPTIIPNPAGDGPSGIVFEDASAGSAGWGDAAAI
                     VPWELWRAYGDLGALAEQLPAMQRWVAYAAGCATAGRHPDRAASRPEPAEHERYLWDT
                     GFHFGEWLEPDVPPAPNPAIDHGIVATAYLHRSAMITAKAAEVLGLTEVAAQNRRMAA
                     GAKAAWQAEYLRPDGRLTEERQAHYVRGLAFDLVPEGLQQAAADRLAELVRQNDDRLN
                     TGFLATGMLLPVLADHGHAVLAYRLLLRTGQPSWLGMIEAGATTMWEYWDGIAPDGQV
                     KGSLDHYSKGAVASFLHTHVAGLRLPEFPTPDWRLAIRRVASDRVR"
     gene            7142..7414
                     /locus_tag="PKJ61_10280"
     CDS             7142..7414
                     /locus_tag="PKJ61_10280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14249.1"
                     /translation="MDGPSSTHPAYGYFRRWYAQVRDWAAVGLADLKARGLYAQPEDA
                     AAQIAGMRDGLDPQWLLDPDSIDLVAVVRSQLQLYTTVDLSSDATE"
     gene            complement(7422..>7685)
                     /locus_tag="PKJ61_10285"
     CDS             complement(7422..>7685)
                     /locus_tag="PKJ61_10285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HOC14250.1"
                     /translation="GSRNFADFIDLMDAVFVLEVDIETLNSRLDARGPDEWGGRPDQR
                     ELILRLHRTQDDVPTDAISIDATAPIERVVDEILRLTEGVAGA"
     assembly_gap    7686..7785
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7907..8521
                     /locus_tag="PKJ61_10290"
     CDS             7907..8521
                     /locus_tag="PKJ61_10290"
                     /inference="COORDINATES: protein motif:HMM:NF018446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HOC14251.1"
                     /translation="MASGATELHPVERMVFFTDAVVAIAMTLLILPLLESVTAAVREG
                     LDTMGYLREHSGQLVAFALSFFIIGRFWRQHDRLFENVTHQSRGLFVLNLVWMFTIVW
                     LPVATAMVGAMQTDALQLAIYIGTMLATSLITLAMQALLLHRAAPDGQPFDRTPLLPM
                     LVTVGLFALALLLALVVPGLHFWSLLILLLSNPAEWLLKRRKPA"
     gene            complement(8475..8969)
                     /locus_tag="PKJ61_10295"
     CDS             complement(8475..8969)
                     /locus_tag="PKJ61_10295"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HOC14252.1"
                     /translation="MRWRARCARPRCCAGLADWGRADFRREYGEVWTAAPKILVSRTR
                     TRTRTSAEHNTRIIGDDAINQLAELPAGTDGDIGVGGATIATQLLRAHLLDELLLFTH
                     PVALGEGRPLFDELDEPVVCDLVEQRSFDWGVTMHRYSVRGAAGHEGVTPASDASVAT
                     PPDC"
     gene            9005..9130
                     /locus_tag="PKJ61_10300"
     CDS             9005..9130
                     /locus_tag="PKJ61_10300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14253.1"
                     /translation="MAAVSQQYSEAELRTIAAYLEAMIDALDVREQRPSGGPAPH"
     gene            9174..>9397
                     /locus_tag="PKJ61_10305"
     CDS             9174..>9397
                     /locus_tag="PKJ61_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007078093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HOC14254.1"
                     /translation="MASESVWSYPRPPAVRPSERLVEVVLGGEVIARTTAAIQVLETS
                     HPPTWYLPSADFAPGALMPGEGASFCEWKGT"
BASE COUNT         1522 a         3319 c         2984 g         1472 t
ORIGIN      
        1 cgctcggagg gactcgaacc cccaaccttc tgatccgtag tcagatgctc tatccattga
       61 gccacgagcg ctggaaagcg ttgtccacct tactcgacgc tgctgcaaag cgtccaactg
      121 ggtggtcaac gcaccctggg catccgagac caagtgcggc ttcgtcaggc tccgccagcg
      181 ttggaggaag ggcttcgtca agctcagccg gcacgaggtc ttggcgggct cgaccaccct
      241 cggcgggaac gccccaacta ggctgtgaga agccaaaacg atgcagcgga tcgcggaatc
      301 gatgcagcat gagacaagcc gcagacaccc ccacaatctc aatcagcagg tggtgataat
      361 gaaacaggat tagtcgaacc tcacaccggg agacccccat tgaggcacga ttgaacctac
      421 cgagcacgca atgcagcgaa cgcggaggag acccatgccc gtggatacca gcacaatcac
      481 caccaacgtc cccgagaacg cgcccgaggc gctcgtcacc tgggtcaacg gtgttgctga
      541 cctcactcaa ccggacgccg tgtactggtg cgacggctcg gtcgacgagc gcgatcgcct
      601 gtacgccgaa atggtcgccg ccggcacctt caccaaactc aaccaggaac tccgtccggg
      661 ctgctatcta gcgcgctcca ccccgtccga cgtcgcccgg gtcgagtccc gcaccttcat
      721 ctgctccgag cgcgaagaag acgccggccc caccaacaac tgggccgacc ccgtcgagat
      781 gaagcagatc ctcgccacga agttcgccgg ctccatgcgc ggccgcacca tgtacgtcat
      841 cccgttctcc atgggacccc tcggcggaga catcagcaag ctcggcatcg aggtgaccga
      901 ttccccctac gtcgtcgtca acatgcgcat catgacccgc atcggcaccc cggccctcga
      961 ccacatcgcc gccggtgagc cctgggttcc cgccgtccac tcggtcggct acccgctggt
     1021 cgacgcggac ggcaacccgc gcgacgacgt cgcctggccc tgcaacgacg acaaatacat
     1081 cacccacttc cccgagaccc acgagatctg gtcctacggc tccggctacg gcggcaacgc
     1141 cctcctcggc aagaagtgct acgccctgcg catcgcctcc gtcctcgccc gcgagcaggg
     1201 ctggctcgcc gagcacatgc tcatcctcaa gatcaccggc ccggacggac gcgagtacca
     1261 cctcaccgca gccttcccgt ccgcctgcgg caagaccaac ctggcgatgc tgcgtccgac
     1321 cattcccggc tggaaggtcg agaccgtcgg cgacgacatc gcctggatgc gccccggcaa
     1381 ggatggacgc ctctacgcca tcaatcctga ggccggcttc ttcggcgtcg cccccggcac
     1441 ctcactcgac accaacccga cctgcctcaa cgccctcgac cacgacgtga tcttcaccaa
     1501 cgtcgcgctc accgacgacg gcgacatctg gtgggaggga ctcaccaagc agcctccggc
     1561 ccacctcatc gactggcagg gcaacgactg gacgccggac tccggccgtc ccgccgccca
     1621 tcccaacagc cgcttcaccg tgcacgcatc ccaggccgag tcaatctccc cggattggga
     1681 ggacccggcc ggcgtgccga tcgacgtcat cctcttcggt ggccgccggg ccaccaacgt
     1741 cccgctgatc agcgagtcct acgactggaa ccacggcgtc ttcgtcggcg ccaccgtctc
     1801 ctccgagcag acggccgccg ccgaaggcgc cgtcggctcg ctgcgtcgcg accccttcgc
     1861 catgcttccc ttctgcggct ataacatggc cgattactgg gcgcactggc tcgagatcgg
     1921 cgaggtcctc ggcgacaagg ccccgcggat cttccaggtc aactggttcc gcaaggactc
     1981 cgacggcaag tggctgtggc ccggcttcgg cgacaactcc cgtccgatcg aatgggcgtt
     2041 gcgacgcgtg aacggcgagg tcggggcagt ggacgcgatc agcggacgca tccccgcgcc
     2101 cggtgacctg aacgtctccg gtctggacat cacggacgcc gaactgaccg ccctgttcga
     2161 gctcgacccg gacgcctggg ccaaggaagc cgacctcacc gaggagtact tcgcccagtt
     2221 cggcgacaag ctccccgccg agctgaccag ccagttggcc ggcctacgcg agcggatcgc
     2281 cgcggctaag tagttgacca ccagacggtg gcccgggctt ggtgcccggg ccatctttgg
     2341 ttcgcttgtg gctgagagat cccggccaac gcccggatga cgtaatgggg tgggacgccg
     2401 ggatgacgta atggggtggg acgccgggat gaccaaccct cacgtcatcc gggcgttggc
     2461 cgggatctcg tttctgccca cgtcatccgg gcgttggccg ggatctctca gcgggccggc
     2521 gacgaactca gccgagcgtc cgcaccgagc cgcgctcgcg cacgggcatc gggatcagcg
     2581 tctgcgcggg agcgtcaccg tcgagcacca gccgcatcgc cgtctggccc atctccaggt
     2641 aggggatgtg catcgtggtc agcccgggcc gcagatacga agccaagtat tcatcatcga
     2701 aggagatgac cgagatgtcg tcgggcaccc gcagcccgcg ctcctgcacc gcctggtaga
     2761 cgccgaacgc tacccggtcg ttggcagcca ccatcgcggt caccgacacc ttgcccagga
     2821 tctcgtgggc gccgcggtag ccgagctcgg gctcccagtc cttgccagcg gtaacttcgt
     2881 ggaggaactc gatgccatcc tcggccagcg cggcgtccat tccggcgtat cgctgggcga
     2941 cggtcgccga gttggccgcc gccatcagca cctgcgatcg tccgatcagc gccagccggc
     3001 gatgcccgcg atcggtcaag tagcgaaccg cgtcatagcc agcttggaat tcatcgggca
     3061 ggacgcacgg gtggccgagc gtgctggtcg cgttcgccat caccaccggc acttcgaccg
     3121 aaagcttcgg cacctggatc tgcttggccg cctgcaaccc gaacacgatg ccgtcagtct
     3181 gccggtcgag catcgcgtcc agtgcctcgg ccatccgcga gatcttgcgt cccgattctg
     3241 agatcaggac ggtgtgctcg cgcttctccg cttcttccag cagcccccgg atgagcgcgg
     3301 acgcgtagcg cgtgatcgtc acctcgtcgg agatgaagcc caccgtccgc gtcttcccta
     3361 gtcgcagccc gcgcgctgcc gggttcggac ggtagttgag ccgggcggcg gcggcccgga
     3421 cgcgctccgc cgcctcttcg gacagccggg tgttggggcg gttgttcagc accatgctga
     3481 ccgtggtcgg ggacaatccg gcaagccggg ccacatcggc cagggtggct cgcttagcca
     3541 tcagcacctc cgccggtctt ctggtgagga aactttaccg tggggccgca tccgattaca
     3601 ccgagggtgg tctcggctgc ctggatcgga cgcagtgcgt cagcggacgg gttccaccgc
     3661 gggccagcgt ccgcgcaggt aggaatccgg gtaccgcgcc atgtggtcgg cgtccagctc
     3721 ggcggcggcc cgcagcggcc aggacggctc gcggagcgcc acccgtccga gcgagatcac
     3781 gtcggcgaga ccatcgtcga ggatcgcctg cgcctgcgcc ggctcggtga tccaccccac
     3841 cgcgctcacc ggcagccccg cttcgcggac ggccgccgcg agcggcacct gatacccggg
     3901 cagcgcgttg atcttcgcca gcacattccc gccgctggac acgtccacca ggtcgacccc
     3961 gtggtcggcc agcatccggg ctacctcggt ggcctcctcg acagtgaacc cgccctcgac
     4021 ccattcggtc gccgagatcc gcaccaacag cggcttaccg gcaggccaga ccgcgcggat
     4081 cgcgtccgcg acctccagca gcagccgcga acgaccggca agatcgccgc cgtattcgtc
     4141 ggtccgatga ttcgacagcg gcgaaaggaa ctggaagatc aggtagccgt gcgcagcatg
     4201 gatctcgacc gcgtcgaagc ccgccgcgtc cgcgcgctga gccgcagctg cgaagtcggc
     4261 caccaccttc cgtagatcgg aaccggtcgc ctcgcgcggc gtggccagcc cggggaacgg
     4321 aatcgcggag ggcccgatgg tctcccagcc gccttcctcg accggcaccg agccggactt
     4381 gcctggctcg aactcgcggt acacggacgc cttgcggccg gcatgctgca actggatcgc
     4441 catcttggcg ccttgggaat gcccgaactc gacgatgcgg cgccaagcat tggtctgctc
     4501 ctcgttccac aagccgcagc agtagggcgt gatccggccc tcaggcgaga cgccggtggc
     4561 ctcggcgatg atcagcccga accccccggc cgcccgggcg ccgtagtgca cgacatgcca
     4621 gtcgttgggc accccatcga cggcctggta ctggcacatc ggcgccagga agatgcggtt
     4681 cgggatggtc acatcgcgca gttgcagcgc attgaacagg gacgacatgg cctcagccta
     4741 tggctgtcgg acgggcgtcc ctgctcgggt taagaccgag cagcgccggt gctggtcgca
     4801 actcagccca gagcggccag ttcggcggcg gtgatgctga gttcgtcggc ggcgatcgcc
     4861 gggtcttcgg cggacgactc gacgcccgcc tgcgggttgc gcgcaggatc ggtctcatcg
     4921 gacccgatcg gacgctaccc gtcgtatcgc cagtcgccaa tccggagtgg ggaattccgg
     4981 aagccgcagc ccggcgacat gcgtgtgcag gaaagacgcc acggcaccct tgctgtagtg
     5041 gtcgagcgaa cccttcacct ggccgtcggg cgcgatcccg tcccagtact cccacatcgt
     5101 ggtggccccc gcctcgatca tgcccagcca ggagggctgg ccggtgcgca gcagcagccg
     5161 atacgccagg actgcatggc cgtggtcggc cagcaccggc agcaacatcc cggtcgccag
     5221 gaagccggtg ttcagccggt cgtcgttctg ccgcaccaac tccgcgagcc gatcggcggc
     5281 tgcctgctgc aggccttcgg gaaccaggtc gaacgccaat cctcggacgt agtgcgcctg
     5341 gcgctcctcg gtcagccgac cgtccggacg caggtactcg gcctgccagg ctgccttggc
     5401 tccggccgcc atccgtcggt tctgcgcggc cacctcggtc agtcccagca cctcggcagc
     5461 ctttgcggtg atcatcgccg atcgatgcag gtaggcggtc gccacgatgc cgtggtcgat
     5521 cgccgggttc ggcgccggcg gcacgtccgg ttcgagccat tcgccgaagt gaaagccggt
     5581 atcccagagg tagcgctcgt gttcggcggg ttctggacga ctcgccgccc ggtcgggatg
     5641 ccgcccagct gtcgcgcagc cggccgcgta ggccacccac cgctgcatcg ccggcagctg
     5701 ctcagccagc gcacccagat ctccgtaggc ccgccacagt tcccacggga cgatcgccgc
     5761 ggcgtccccc cagccggccg aaccggcgga cgcgtcctcg aacacgatgc cggacggtcc
     5821 gtccccggcg gggttgggga tgatcgtcgg aatccggccg tcggcccact ggtcggcggc
     5881 caggtcgcgc agccagcgcc gtgaaaaacg ctgcacgtcg gccatcatcg cggccgtgct
     5941 gacgaagagc tgccagtcgc cggtgaaccc ggaacgctcc cgctgcgggc agtcggtcgg
     6001 gatcgcgcag acgttgttgc gcaggctcca gcgcaccact tcgtgcagcc gcgccagccg
     6061 cgcgtccgag cagccgaatt ccccggtccg gcgcaggtcg gagtgcacca ccacgccgcg
     6121 cgcgctgctg agatcaaggt cgtccgggac cccgtccacc tgcacgaagc ggaagccgtg
     6181 ggtggtgtgg cgcggctcga aactctcgcc gaccaggccg gacgcgatca cctcgtccac
     6241 ttgtccggcc ggcagcacct gcccggttgc gaacacgaac gcgcgcaggt ggtcggtatt
     6301 caccagtccg gacactgcca gggcctcacc gtgggtgagc acgagccgcg tcccggccgg
     6361 cccaaggtcg gtgagccgca cccagccgtt gatcgcctgg ccgaagtcga gcaccaccgt
     6421 gcccggacgc ggacgcgtga ccgcgactgg cgcgagctcc tgagtccgcc gcaccggcgg
     6481 cgcgatcggc gcgaccaggc gggaccggtc tgcgtagatc ggcccttccg ggaccaccac
     6541 ctgctgccag cccgggccgt ccgcgccggt gaaccagtca gggtcgagca ggcgcagatc
     6601 agtcgtttgg ccgtccatca ggtcggcgcg caggatgtgc gatggtcccg actcccatcg
     6661 cggcccggtc gccaggatcg gttcgcccgc gtcatcggtc agcgcgagca gcagcgcggt
     6721 ctggtcgccg aagccgtccg cccgacgctc gaagccgtgc cttcctcggt accagccgtc
     6781 cgacaacacc accaccaggg tgtttgggcc cggcaccacc agctcgctga cgtcgcaact
     6841 ctgcacctcg aggcgcttgc ggtacgcgga gaagccgggc gccagttcca tgtcgccaat
     6901 tcgggaaccg ttgaggaatg cctcgtagag gccgtgcgcg gtggcgtgaa ccagcagcga
     6961 tttcgcgaca tcggacgcgt cgaagcacgc tcgtagcaga tacgccggac gcttaccggg
     7021 ctccccgggc gccttatcag gcgcgatcca ctgcgcgctg gccagcccgg cccgcacctg
     7081 cacatcagtc agtgcgagca cgtcgacggc aacaccgaat tctgcagcga ggggacgagc
     7141 attggacggt ccttcctcca cccaccccgc gtacggctac ttccgacgct ggtacgccca
     7201 ggtccgggat tgggccgcgg ttggcctggc cgacctgaag gcacgcgggc tgtacgccca
     7261 gcccgaggac gcggccgcgc agatcgccgg catgcgtgac ggcctcgacc cgcagtggct
     7321 gctcgatccg gactccatcg acctggtggc cgtggtgcgg tcgcagttgc agctctacac
     7381 cacggtcgat ttgagctcgg acgcgaccga gtagctcgcc gttaggctcc ggctacacct
     7441 tcggtgagtc ggaggatctc gtccacgacc cgttcaattg gtgcggtggc gtcgatgctg
     7501 atcgcatccg ttggcacatc gtcctgagtg cggtgcaggc gcaggatgag ttctcgttgg
     7561 tccggacgtc cgccccactc gtccgggcca cgggcgtcga gccgcgagtt cagggtttcg
     7621 atgtccactt cgaggacgaa gactgcgtcc atcagatcga tgaagtcggc gaagttccgg
     7681 gacccnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnaccgt gacccagaac
     7801 atgatcgcca agaccgtcca cgagtccacg tccctcaggc tagcgaagcg gtatccgcga
     7861 cgggcgcccc cttgcgtagt ccggccgtga ccgctaggcc ttgatcatgg ccagtggtgc
     7921 aactgaactc cacccggtgg agcggatggt gttcttcacc gacgcggtgg tcgcgatcgc
     7981 gatgaccctg ctgatcctgc cgctgctgga atcggtgacc gcggccgtcc gcgaaggcct
     8041 ggacaccatg ggctacctgc gcgagcacag cggccaactg gtggccttcg ccctgagctt
     8101 cttcatcatc ggtcggttct ggcggcagca cgaccggctg ttcgagaacg taacccacca
     8161 gagccgtggg ctgttcgtgc tcaatctggt ctggatgttc accatcgtct ggctgcccgt
     8221 cgccaccgcg atggtgggcg ccatgcagac cgatgcgctg cagttggcca tctacatcgg
     8281 cacgatgctc gccaccagcc tgatcaccct cgccatgcag gccctgctgc tgcaccgggc
     8341 ggcgccggac gggcagccgt tcgatcggac cccgctgctg ccgatgctcg tcactgtcgg
     8401 gctgttcgcg ctcgccctcc tgctggctct cgtggttccc ggcctgcact tctggagcct
     8461 gttgatattg ctgctcagca atccggcgga gtggctactg aagcgtcgga agccggcgtg
     8521 acgccctcgt gtcctgcggc accgcggacg gagtagcggt gcatggtgac gccccagtcg
     8581 aaggaccgct gctcaaccag gtcgcagacg actggttcgt cgagctcgtc gaagagcgga
     8641 cggccctcgc ccagagccac cggatgggtg aagagcagca gttcgtcgag caggtgtgct
     8701 cgcagaagct gggtggcgat ggttgccccg ccgacgccga tgtcaccgtc ggttcccgcc
     8761 ggcagctcgg ccaactgatt gatcgcgtcg tcgccgatga tgcgggtgtt gtgctcggcg
     8821 ctggtccgcg tccgcgtccg cgtccgcgaa accaggatct tgggtgcggc ggtccagacc
     8881 tcgccgtatt cgcgccggaa gtcggcgcgt ccccaatccg cgagaccggc gcagcatcgt
     8941 ggtcgagcac accgcgcacg ccaccgcaac cggcctagcc catttagagt cgatcgccga
     9001 gcggatggct gcggtcagtc agcagtacag cgaagctgag ctacgcacca tcgccgctta
     9061 cctcgaggcg atgatcgacg ccctggacgt acgcgagcag cgaccctcgg gcggtccagc
     9121 gccgcactga ctcgattcgc cggctttgcc ggatctggca atctggagca atggtggcaa
     9181 gcgaatccgt ttggagctac cccaggccgc ccgccgtccg accgagcgaa cgcctggtag
     9241 aggtcgtcct cggcggcgag gtgatcgccc ggacgaccgc ggcgatccaa gtactggaga
     9301 ctagccatcc gcctacctgg tatctgccga gtgcggattt cgcacccggc gcgctgatgc
     9361 ccggcgaggg agccagcttc tgcgaatgga agggcac
//
LOCUS       DAOPRQ010000086         9372 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_6151_length_9372_cov_11.143286, whole genome shotgun sequence.
ACCESSION   DAOPRQ010000086 DAOPRQ010000000
VERSION     DAOPRQ010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 9372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9372
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6151_length_9372_cov_11.143286"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..164
                     /gene="rpsO"
                     /locus_tag="PKJ61_10310"
     CDS             <1..164
                     /gene="rpsO"
                     /locus_tag="PKJ61_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOC14255.1"
                     /translation="AHLTEHLKEHKGDHHSRRGLMLLVGQRRRLLNYVMKNDVEHYRS
                     LIARLGLRR"
     gene            350..2572
                     /locus_tag="PKJ61_10315"
     CDS             350..2572
                     /locus_tag="PKJ61_10315"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOC14256.1"
                     /translation="MEGPDLQYTEAVIDNGSLGKHVVRFESGLLARQANGCATVYLDG
                     DTMLLSATTAAKNPRDAIDFFPLTVDVEERMYAAGRIPGSFFRREGRPSEGAILAARL
                     CDRPLRPCFVKGLRNEVQVVVTVMALNPNVLYDVLAINASSASTTLAGLPFSGPVGGV
                     RVALIGDQWVGFPTVEQLEGATFDMVVAGRVLPDGDVAIMMVEAESTEATWDIVRSGR
                     TAPTEEVVAQGLEASKPFIKALCDAQAELAARFDKPVSQFPVFLDYQSDVFDAVAEAG
                     TERLKSVMSIAGKAERETTTEELLATLHSELDARFPDREKEITGAYRALTKKVVRHRT
                     LTEQVRIDGRGVRDIRTLSAEVGVLPRVHGSALFMRGETQILGVSTLNMLDMEQKLDT
                     LAPETTKRYMHNYNFPPYSTGETGRVGSPKRREIGHGALAERALVPVLPKRDEFPYAI
                     RQVSEALGSNGSTSMGSVCASTLALLNAGVPLKAPVAGIAMGLMSEEIDGETKYVALT
                     DILGAEDALGDMDFKVAGTKDFVTALQLDTKLNGIPADVLAGALLQAREARHTLLEVM
                     GEAIDTPDEMSPYAPRIITVRIPVDKIGEVIGPKGKMINQIQDDTGANISIEDDGTIY
                     IGADNGESAEAARALINAIANPTMPEKGERYLGTVVKIMPFGAFISLLPGKDGLLHIS
                     KLRGLAGGARVENVEDVVSVGQKIQVEISDIDEKGKLSLIPVVEEKAAEAPAEVPAEA
                     "
     gene            complement(2691..3311)
                     /locus_tag="PKJ61_10320"
     CDS             complement(2691..3311)
                     /locus_tag="PKJ61_10320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14257.1"
                     /translation="MTPSTKRRGRTMTEPPDLPELWRTQREQATHRAHAQYCRGHGWL
                     ADAGLDTVLAPLLCGPMYGDDAFFRFAALGADSAAALLAALPEEYLATERQNDSPTIG
                     KILRAVVAHPDRLLAHGYVIGPARCDERITTEGVLLRTGREYRLCELYGPPTPDCECA
                     DLYRVLVDDFGVDDAHTHPHELDRFHYYEWTPDGYQGEPWYRAWWD"
     gene            3473..4207
                     /gene="dapB"
                     /locus_tag="PKJ61_10325"
     CDS             3473..4207
                     /gene="dapB"
                     /locus_tag="PKJ61_10325"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HOC14258.1"
                     /translation="MRVAVFGAKGRMGSEVCRAVTATPDLELVAALDVGDDRTPAETA
                     DVVVDFTVPDAVMANLAWCIEHDKNVVVGTTGFTDARLDEVRAQLADHPQVGVLVAAN
                     FSIGAVLMMHFAERAARFFESAEIIELHHPNKVDAPSGTATVTAQRIASARAAAGIGP
                     VPDATTTQLPGARGADIDGIRVHGVRLRGLVAHQEVLFGAAGETLTIRHDSLDRSGFM
                     PGVLAAVRAVTGLPGLTIGIDGLLGL"
     gene            4209..4901
                     /locus_tag="PKJ61_10330"
     CDS             4209..4901
                     /locus_tag="PKJ61_10330"
                     /inference="COORDINATES: protein motif:HMM:NF022655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2993 domain-containing protein"
                     /protein_id="HOC14259.1"
                     /translation="MRRALIWLVVVALLSAGAVFADSWLRGYVQDQAEPQIGTQLGAG
                     EDLEVTLGGVPFSLALLTRSVPSATLTADEVKLDVAGKELRLRQASITTGRVSLADTQ
                     VDATNLVGAAELSYADLEKLAGVPVQYADDGRLELRYTASLLGRELGLAVSALPVLDE
                     ARAKIQLTDPKLDLNGSEVDLPIEQGLVDSLVEPIDVKLDYGLAVTALIPAESGLSVA
                     FGGDAVSIPLGE"
     gene            4953..6284
                     /locus_tag="PKJ61_10335"
     CDS             4953..6284
                     /locus_tag="PKJ61_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOC14260.1"
                     /translation="MTQTDWILLAVALACVLLAALVTTAETALSSVSKSRAEKLLADG
                     RPGAKRLLLQAQDPAPYLNTSLFVRLLLEAVATVLVTLVFFGRFTPTWERILFPVVTM
                     VLVSFILLGVAPRTLGRQHAEAVALAASGPIAALETVLGPIPQLMIVIGNALTPGRGF
                     SDGPFTSEAELRELVDIAEAREVIEDDERRMIHSVFELGDTIVKEVMVPRTEMVYIEQ
                     GKTLRQGVSLALRSGFSRIPVIGEDVDDVVGVLYLKDIIRRMYDNPKAQTNETVDTLM
                     RAPSFCPDSKPVDELLREMQLTRSHVVIVIDEFGGTAGLATIEDVLEEIVGEIVDEYD
                     HEVPPITELGESRFRVSARLGIDDLAELFGLRADDEDVDTVLGLMARELNKVPIPGSV
                     VEWEGIELVAERGGDRRHTVQTVLASPATENGDAAVEAAAKLAEESAKRVS"
     gene            6281..7246
                     /gene="era"
                     /locus_tag="PKJ61_10340"
     CDS             6281..7246
                     /gene="era"
                     /locus_tag="PKJ61_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HOC14261.1"
                     /translation="MSEHPGTGSQPAGAGERLSHSGFACFVGRPNAGKSTLTNALVGT
                     KIAIASSKPQTTRHAIRGIVNRPDAQLIVIDTPGLHKPRTLLGERLNDLVRDTWTEVD
                     VVGVCLPANQRIGPGDEYLVSELVSLAKKAQLVALATKSDLVSPARMAEHLLKIASLE
                     EKFGIRWASIIPVSAVTDDQVDVVTDELVKLLPPGPRFYPEGEVTDEPTETLVAELIR
                     EAALEGVRDELPHSLMVVVEEMGERAGRDPDRPLLDIFASMIVERDSQKGIMIGHKGE
                     RLREVGTTARQQISALVGMPVHLDLRVKVLKEWQRDPKHLNRLGF"
     gene            7457..9244
                     /gene="leuA"
                     /locus_tag="PKJ61_10345"
     CDS             7457..9244
                     /gene="leuA"
                     /locus_tag="PKJ61_10345"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014604479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HOC14262.1"
                     /translation="MTINTFGTRPQPVPTQQPSPMPFNRYTPFEPIDVPDRTWPAKKI
                     TAAPRWLSTDLRDGNQALIDPMTPSRKRKMFDLLVRMGYKEIEVGFPSASQTDFDFVR
                     SLITSDAIPEDVTISVLTQAREDLIERTAQSLIGARRANIHLYNATAELFRRVVFGID
                     EAQCIAMAVRGTELVMKYAEANLGEVEFGYQYSPEIFTQTPTDFAIEVCNHVMDAWQP
                     SAGREIILNLPATVERSTPNTYADQIEYFGRHVRNRDHVAISLHPHNDRGTAVAATEL
                     ALMAGADRVEGCLFGHGERTGNVCLVTLGMNLFSQGIDPKIDFSDIDEIRRTVEYCTN
                     LPVHPRHPYAGDLVYTAFSGSHQDAIKKGLEVLERTAASQGKTLHEIPWEAPYLPIDP
                     HDVGRTYEAVIRVNSQSGKGGMAYIMKSEHSMDLPRRLQMEFSRVVQARTDSAGGEVA
                     PDEMWTIFSAEYLEDTAPLELVTVKSVSNGGGYTVSAVVRDHGVEKEIVGEGNGPVSA
                     FVDALGILGYHVRVLDYTEHALSSGGDAKAAAYVETELGSDEDAHVLWGVGLHESIVT
                     ASLRAVISAINRDARIQAAQTQEQDTPRP"
BASE COUNT         1577 a         3102 c         3142 g         1551 t
ORIGIN      
        1 tcgcgcacct gaccgagcac ctcaaggagc acaagggcga ccaccacagc cgccgtggcc
       61 tgatgctgct ggtcggccag cgccgccgcc tgctcaacta cgtgatgaag aacgacgttg
      121 agcactaccg ttcgctgatc gcacgcctcg gcctgcgtcg gtgatttcag cttgacgacc
      181 agccggtacg ccgatcaggc gtaccggctg gttgtgcatc cacaacaaaa tcgccgtgac
      241 ggactggttt ggtcctcggt agtggctttc gggcttccgg acggaatccc gcgggcttcg
      301 atcgaagacc ggacgagatt ccaccgcggc agacccgaaa ggaatccgaa tggaaggtcc
      361 tgaccttcag tacaccgagg ccgtgatcga caacggctcg ctcggcaagc acgtcgtccg
      421 gttcgaatcc ggcctcctgg cccgtcaggc caatggctgc gccaccgtct acctcgacgg
      481 cgacaccatg ctgctctcgg cgaccaccgc ggccaagaac ccgcgcgacg ccatcgactt
      541 cttcccgctg accgtggacg tggaggagcg gatgtacgcc gccggccgca tccccggctc
      601 cttcttccgc cgcgagggtc ggccctccga gggcgccatc ctggctgctc gcctgtgcga
      661 ccgtccgctg cgtccctgct tcgtcaaggg cctgcgcaac gaggttcagg tcgtcgtcac
      721 cgtgatggcg ctcaacccga acgtgctcta cgacgtgctg gccatcaacg cctcctctgc
      781 ttccaccacg cttgccggcc tgccgttcag cggcccggtc ggtggcgtcc gcgtcgccct
      841 catcggcgat cagtgggtcg gtttcccgac cgtggagcag cttgagggcg ccactttcga
      901 catggtcgtg gccggccggg tgctgccgga cggcgatgtc gcgatcatga tggtcgaggc
      961 cgagtccacc gaggccacct gggacatcgt ccgctccggc cgtaccgccc ccaccgaaga
     1021 ggtcgtggcg cagggcctgg aggcgtccaa gccgttcatc aaggcgctgt gcgacgcgca
     1081 ggctgagctc gccgcccggt tcgacaagcc ggtcagccag ttcccggtct tcctcgacta
     1141 ccagtccgat gtcttcgacg cggtcgccga ggccggcacc gaacggctca agtccgtgat
     1201 gagcatcgct ggcaaggctg agcgcgagac caccaccgag gagctgctgg ccaccctgca
     1261 cagcgagctc gacgcccgct tccccgaccg ggagaaggag atcaccggcg cctaccgtgc
     1321 gctcaccaag aaggtcgtgc ggcaccgcac gctgaccgag caggtgcgca tcgacggtcg
     1381 tggtgtccgg gacatccgca ccctctcggc tgaggttggc gtgcttccgc gggtgcatgg
     1441 ttcggcgctg ttcatgcgtg gggagaccca gatcctgggc gtctccaccc tgaacatgct
     1501 cgacatggag cagaagctcg acaccctggc gccagagacc accaagcgct acatgcacaa
     1561 ctacaacttc ccgccgtact ccaccggtga gaccggccgc gtcggttcgc cgaagcgccg
     1621 cgagatcggc cacggcgcgc tcgccgagcg tgcattggtg ccggtgctgc cgaagcggga
     1681 cgagttcccc tacgccattc gccaggtgtc cgaggccctc gggtcgaacg gctccacctc
     1741 gatgggctcg gtatgcgcat ccaccctggc gctgctgaac gcaggcgtgc cgctgaaggc
     1801 tccagtggcg ggcatcgcca tgggcctgat gagcgaagag atcgacggcg agacgaagta
     1861 cgtcgctctc accgacatcc ttggcgctga ggatgccctc ggtgacatgg acttcaaggt
     1921 ggccggcacc aaggacttcg tgaccgcgct gcagctcgac accaagctga acggcattcc
     1981 tgccgacgtg cttgccggtg cgttgctgca ggcccgcgag gcccgacaca cgctgctgga
     2041 agtcatgggt gaggccatcg acaccccgga cgagatgagc ccctacgcgc cgcggatcat
     2101 cacggtgcgc atcccggtcg acaagatcgg cgaggttatc ggccccaagg gcaagatgat
     2161 caaccaaatc caggacgaca ccggcgccaa catctcgatc gaggacgacg gcacgatcta
     2221 catcggtgcg gacaacggcg aatccgccga ggccgcccgc gcgctgatca acgccatcgc
     2281 caacccgacg atgccggaga agggcgagcg ttacctgggc acggtcgtga agatcatgcc
     2341 gttcggtgcg ttcatctcgc tccttccggg caaggacggc ctgctgcaca tctccaagct
     2401 gcgcggcctg gccggcggcg ctcgcgtgga gaacgtggag gacgtcgtca gcgtcggcca
     2461 gaagatccag gtggagatct ccgacattga cgagaagggc aagctctccc tgatccccgt
     2521 ggtcgaggag aaggctgccg aggccccggc cgaggtgccc gccgaggcct gatccaggct
     2581 gcgagccggc gtcccgcgat cgcgggacgc cggctttcgc gctccctgcc cggtcggtcg
     2641 ctcctgtaga gacctactgc ggtctcgaca agctcgatca gccgagcggt tcagtcccac
     2701 cacgcccggt accagggctc gccctgatag ccgtccggcg tccactcgta gtagtggaag
     2761 cggtccagct catgcgggtg ggtgtgcgca tcgtcgacgc cgaagtcgtc gacgagtacg
     2821 cgatagagat ccgcgcactc gcaatccgga gtcggcggtc cgtacagctc gcacagccga
     2881 tactcgcgcc cggtacgcag caggacgcct tcggtggtga tccgctcgtc gcagcgagcc
     2941 ggaccgatca cgtagccatg ggccagcagc cggtcggggt gggcgaccac cgcgcggagg
     3001 atcttgccga tggtggggct gtcgttctgc cgctcggtgg cgaggtactc ctccggcagt
     3061 gcggccagca atgcggctgc gctgtccgca ccgagtgccg cgaatcgaaa gaacgcgtcg
     3121 tcgccgtaca tcgggccgca gagcagtggg gcgagcacgg tgtccagacc ggcgtcggcc
     3181 agccagccgt gcccgcggca gtactgggca tgtgctcggt gtgtggcctg ttcgcgctgg
     3241 gtgcgccaca actcgggtag gtccggcggc tccgtcatcg tcctgccgcg gcgcttggta
     3301 ctcggtgtca tcgatcctcc ttgacgttct cgatgacacc ttcggcaagt gcccgcaatt
     3361 tccttcacct gacttcaccg cctgtggaca acttcgggcc tgtggacaga cgcggtcggg
     3421 cctgtcagtg ccgtctccta gacttgccgg cacgcgagag ggaggggtcg gcatgcgagt
     3481 cgccgtgttc ggagccaagg ggcgaatggg atctgaggtc tgccgtgcag tgacggccac
     3541 gcccgatctg gagttggtgg ccgctctcga tgtcggcgac gaccggacgc cggctgaaac
     3601 agctgacgtt gtggttgact tcactgttcc ggacgcggtc atggcgaacc tcgcctggtg
     3661 catcgagcac gacaagaatg tcgtggttgg caccaccggc ttcaccgatg cccgcctgga
     3721 cgaggtgcgg gctcaactcg ctgaccaccc acaggtgggc gtgctggtgg ccgcgaactt
     3781 ctcgatcggc gcggtgctga tgatgcactt cgccgagcgg gcggcccggt tcttcgagtc
     3841 ggccgagatc atcgagctcc atcacccgaa caaggtggat gcgccgtccg gtacggccac
     3901 cgtcaccgcg cagcggattg cgtccgcccg cgcggccgcg ggcatcggac cggtgccgga
     3961 cgccacgacc acgcagctgc ccggagcgcg cggggcagac attgacggga ttcgtgtgca
     4021 cggcgtccga ctgcgcggac tggtggcgca tcaggaagtg ctgttcggcg cggccggaga
     4081 gacgctcacc atccggcacg actcgctgga ccgatccggg ttcatgcccg gcgtgctggc
     4141 agcggtccgt gcggtaaccg ggctgcccgg gttgaccatc ggcatcgacg gcctgctggg
     4201 cttgtgagat gcgaagggcg ctgatctggc tggtagtggt ggcactgctg agcgccggcg
     4261 cggtctttgc cgactcctgg ctgcgcggct atgtacagga tcaggccgag ccgcagatcg
     4321 gtactcaact tggtgccggc gaagatcttg aggtgaccct cggcggggtg ccgttcagcc
     4381 tggccctgct gacccgcagc gttcccagcg ccaccctgac cgcggacgag gtgaagctcg
     4441 acgtcgccgg caaggaactc aggcttcgcc aggccagcat taccacggga cgggtgtcgc
     4501 tcgcggatac gcaggtggat gccacgaacc tggtcggtgc cgcagagctc agctacgccg
     4561 atctggagaa gctggctggg gtaccggtgc agtacgcgga cgacggccgc ctcgagttgc
     4621 gctacaccgc gagcctgctc ggccgcgagc ttggcctggc ggtctcggcg ctaccggtgc
     4681 tggacgaggc aagggccaag atccagttga ccgacccgaa gctcgacctg aacggttctg
     4741 aagtcgacct gccgatcgag cagggcctgg tcgactcgct ggtggaaccg atcgacgtga
     4801 agctcgacta cgggttggcg gtgactgcgc tcattccggc cgaatccggc ctgagtgtgg
     4861 ctttcggtgg cgacgcggta tccatcccgc tgggggagta gcccgtccac tagactgggc
     4921 gctcaccgaa ctgagcgagg cgtgaacacc cgttgaccca gaccgactgg atcctgttgg
     4981 ccgtggctct tgcctgcgtc ctgctggccg cactggtgac caccgctgag accgcgttgt
     5041 cctcggtttc gaagtcccgg gccgagaagc tgctggcgga cgggcgtccg ggcgccaagc
     5101 ggttgctgct gcaggcgcag gacccggcgc catacctgaa cacctcactc ttcgtccgcc
     5161 tgctgctcga agcggtggcc acggtgctgg tcaccctggt cttcttcgga cggttcacgc
     5221 ccacctggga gcgcatcctc ttcccagtcg tgacgatggt gctggtgtcg ttcatcctgc
     5281 tcggggtggc tccgcgcacg ctcggacgcc agcacgccga agctgtggcg ctggccgcgt
     5341 ccgggccgat cgcggcactc gagaccgtgc tcgggccgat cccgcaactg atgatcgtga
     5401 tcggcaacgc gctcaccccc ggacgcggct tcagcgacgg gccgttcacc tccgaggccg
     5461 agttgcgcga gctcgtcgac attgccgagg cgcgcgaagt gatcgaggac gacgagcgtc
     5521 gcatgatcca ctcggtcttc gaacttggtg acacgatcgt caaggaagtc atggtgccgc
     5581 gcaccgagat ggtctacatc gagcaaggaa agactctgcg gcaaggggtt tcgcttgccc
     5641 tgcgctcggg cttcagccgg atccccgtca tcggtgagga cgtcgacgat gtcgtcggcg
     5701 tgctctatct gaaggacatc atccgccgga tgtacgacaa tccgaaggcc cagaccaacg
     5761 agaccgtaga caccttgatg cgtgcgccga gcttctgccc ggactccaag ccggtggacg
     5821 aactgctccg cgagatgcag ctcacccgca gccatgtggt gatcgtgatc gacgagttcg
     5881 gtggcaccgc cggcctggcc acaatcgagg acgtgctgga ggagatcgtc ggcgagatcg
     5941 tcgacgagta cgaccatgaa gtgccgccga tcactgagct gggggagagc cgcttccggg
     6001 tgtcggcgcg gctgggcatc gacgacctgg ccgagctctt cggcctgcgc gccgacgacg
     6061 aggacgtcga cacggtgctg gggctgatgg ccagggagct caacaaggtg ccgatcccgg
     6121 gttcggtcgt ggaatgggaa ggcatcgagt tggtcgccga gcgcggcggt gaccggcgac
     6181 acaccgtcca gacggtcctg gcttcaccgg cgaccgagaa cggcgacgca gcagtggaag
     6241 cggcggccaa gctcgccgag gaatcagcga agagagtctc atgagcgagc atccaggcac
     6301 cggatcacaa ccagccggcg cgggggagcg actgtcccat tccggcttcg cctgctttgt
     6361 cggacgcccg aacgcgggca agtcgacgct gaccaatgcc ctggtcggta cgaaaatcgc
     6421 gatcgcgtcc tcgaagccac agaccacccg gcacgcgatc cgcggcatcg tgaaccgtcc
     6481 cgacgcgcag ctgatcgtga tcgacacccc cggcctgcac aagccgcgga cgctgctggg
     6541 cgagcggctc aacgacctgg tccgcgacac ctggacggag gtcgacgtgg tcggcgtctg
     6601 cctgcccgcc aatcagcgga tcggtccagg cgacgagtac ctggtctccg agttggtctc
     6661 gctggccaag aaggcccaac tggtggcgct ggccaccaag tccgacctgg tctcaccggc
     6721 gcggatggcc gagcacctgc tgaagatcgc ctccctggag gagaagttcg gtatccgctg
     6781 ggcgagcatc atcccggtct cggccgtgac cgacgaccag gtggacgtgg tcaccgacga
     6841 gttggtcaag ctgctgccac ccggcccgcg gttctatccc gagggcgagg tcaccgatga
     6901 gccgaccgag actctggtgg ccgaactcat tcgcgaagcc gcgctcgagg gcgtccggga
     6961 tgaactgccg cactcgctga tggtggtggt ggaggagatg ggcgagcgtg ccggacgcga
     7021 ccccgaccgt ccgctgttgg acatcttcgc ctcgatgatc gtcgagcggg attcgcagaa
     7081 gggcatcatg atcggccaca agggagaacg cctgcgcgag gtcggtacca ccgcgcgcca
     7141 gcagatctcc gcgctggttg gcatgccggt gcacctcgac ctgcgggtca aggtgctcaa
     7201 ggagtggcag cgggacccca agcacctgaa ccgactgggg ttctgaaggg cttcgtcgcg
     7261 ctcggccatc gtcggcggca gcgtccacat ggtgtgccgc ccttggccct gcgccgcgtg
     7321 atgtgcaacg atgggcgaca tgtcatgggt gcgactcctc cttggtcgcc gcagcgaggc
     7381 ctagaagtcc tgatcaggcc tcctcgctgc ggagttctgc acgcgcctgg caccagcccg
     7441 cacagcgagg acaccaatga ccatcaatac gttcgggacg cgtccgcaac cggttccgac
     7501 tcagcagccc agcccgatgc cgttcaaccg gtacacgccg ttcgagccga tcgacgtgcc
     7561 cgatcggact tggccggcca agaagatcac cgccgcgccg aggtggctgt ccaccgatct
     7621 gcgcgacggc aaccaggcgc tgatcgaccc gatgacgccg tcccgcaagc gcaagatgtt
     7681 cgacctcctg gtgcggatgg gttacaagga aatcgaggtc gggttcccgt ccgcttcgca
     7741 gacggacttc gacttcgtgc gcagcctgat cacctctgac gcgattcccg aagatgtcac
     7801 catcagcgtg ctcacccaag cccgtgagga cctgatcgaa cgcaccgcac agtccctgat
     7861 cggggctcgg cgggcgaaca ttcacctgta caacgcgacc gcggaactgt tcaggcgggt
     7921 ggttttcggc atcgacgagg cgcagtgcat cgccatggcg gttcgcggca ccgaactggt
     7981 gatgaagtat gccgaggcga acctgggcga ggtggagttc ggctaccagt acagcccgga
     8041 gatcttcacc cagaccccga cggatttcgc catcgaggtg tgcaaccacg tgatggacgc
     8101 ctggcagccg agcgccggcc gggagatcat cctgaacctg ccggccacgg tcgagcgctc
     8161 gacgcccaat acctacgccg accagatcga gtatttcgga cgccatgtgc gcaaccgcga
     8221 ccacgtggcg atctcactgc atccccacaa cgaccgggga accgcggtgg cggccaccga
     8281 gttggcgctg atggctggcg ccgaccgggt cgagggttgc ctgttcggcc acggcgagcg
     8341 gacgggaaat gtgtgcctgg tgaccctggg catgaacctg ttcagccagg gcatcgaccc
     8401 gaagatcgac ttctccgaca tcgacgagat tcgtcggacg gtggagtact gcaccaacct
     8461 gccggtccat ccgcgccacc cgtacgcggg ggacctggtc tacaccgcct tctccggctc
     8521 ccaccaggac gccatcaaga aggggctgga ggtgctggaa aggacggccg cctcccaggg
     8581 gaagactctg catgagattc cctgggaggc gccgtacctg ccgatcgatc cgcacgacgt
     8641 cgggcgcact tacgaggccg tcatccgggt gaacagccag tctggcaagg gcggcatggc
     8701 ctacatcatg aagtccgagc attcgatgga cctgccccgc cggctgcaga tggagttctc
     8761 ccgcgtggtg caggcccgca ccgactctgc gggcggcgag gtggcgccgg acgagatgtg
     8821 gacgatcttc tccgccgagt acctggagga caccgccccg ctggagctgg tgaccgtcaa
     8881 gtcggtctcc aacggcggcg gctacacggt ctctgcggtg gttcgcgatc acggggtcga
     8941 gaaggagatc gtcggtgagg gcaacggccc ggtgtcggcg ttcgtggacg ccctcggaat
     9001 cctcggctac cacgtccgtg tgctggacta caccgaacat gcgctctcgt ccggcgggga
     9061 cgcgaaggct gcggcctacg tcgagaccga gctaggtagc gacgaggatg cgcacgtcct
     9121 ctggggtgtc gggctgcacg agtcgatcgt caccgcgtcg ctgcgtgccg tgatcagcgc
     9181 gatcaaccgg gacgcccgga tccaggccgc ccagacccag gagcaggaca ccccgcgtcc
     9241 gtagcttccc tggctgagcc tgacgaagcc tcgcggcgtc cgcccgtgcc ccgcgtctcg
     9301 ccgagcgcca gccctaggga aacgctgatc aatgttcgag ctgttgcgct cgggtggtgg
     9361 cgttgttgac tg
//
LOCUS       DAOPRQ010000087         9050 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_6412_length_9050_cov_10.149305, whole genome shotgun sequence.
ACCESSION   DAOPRQ010000087 DAOPRQ010000000
VERSION     DAOPRQ010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 9050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9050
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6412_length_9050_cov_10.149305"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="PKJ61_10350"
     CDS             <1..494
                     /locus_tag="PKJ61_10350"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HOC14263.1"
                     /translation="VEARYDKMHLFDALGYAESRQIQPGTQPVHFDLAGTRIGLATCY
                     DVRFPQLFTHLAGTGAELILLPASWAPGEHKLHQWRTLVTARAMDATAFVVAVDQALS
                     PAAADGRTPTGIGHSMVVDPTGDVLLELGDDPEFAVIDLNLAQVAEVRRRLPVLEHTR
                     RFN"
     gene            644..2386
                     /locus_tag="PKJ61_10355"
     CDS             644..2386
                     /locus_tag="PKJ61_10355"
                     /inference="COORDINATES: protein motif:HMM:NF024594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4012 domain-containing protein"
                     /protein_id="HOC14264.1"
                     /translation="MRKRRWPWIALTAVVLTVAAVGISGFLVYQDAMRARDSLQVAAD
                     ALPQVATQIRSDPQRADATIARVQMAAHSALEATTGPHWTVAGWLPWVGDNTRALQTV
                     TTAVDRIASGVLPELAASVQSVTPERLAPQDGKLNLAPLVEVRERVVDADQIVGQAIT
                     DVSGIPSANLIDQLADAVARLQQELTDARALTSTASAAVQLLPAMLGADGPRDWLLLA
                     QNNAESRATGGIPGAAILLHADNGRLSIVKQLGAGDFGPYPEPILPLTADESRLFGAA
                     LGHYLQDVNLTPDFPRSGQLVRAIWAADGGASDVANVVSIDPVALQTILRATGPVQFT
                     DPRGHAITLDADNTASFLMSEVYRRFDSPDAQDAVFALAVQAVLQQVMSGEADAGELL
                     GAVTEIAGNGRLLVWSADASEQQQLETIGVTGALRGSIQAGEGTRPVVGVFLNLTTAS
                     KLGYYLDLKATIGEVKARPDGSREFDVTVRLKSRLTPAEARRLPSHVIENAPRDGTNR
                     VNVLVYAPTDGTITQLSTASPGFVTTHDGLQVSAQTVTVPPESAAEVRFHIVTGPGQD
                     AEPYLRQTPGARNA"
     gene            2541..3137
                     /locus_tag="PKJ61_10360"
     CDS             2541..3137
                     /locus_tag="PKJ61_10360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14265.1"
                     /translation="MNLRALGTSIAATALVFAFPAAAYAETASPDPGPETTVPNYEAR
                     EYCKITANPTSLQAGGTTQISLVDAPNAETDLSVTSADASVKDSAIQIAGKQTAVKKA
                     DANGNVSFSVTLAEPGDYILDASTITGQFCGLTVKVSPKEKVVEAAEDEDETPVANAA
                     AAVLANTGATTLPYVGAAVVLLGAGAVAVVATRRRRNS"
     gene            3324..3656
                     /locus_tag="PKJ61_10365"
     CDS             3324..3656
                     /locus_tag="PKJ61_10365"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HOC14266.1"
                     /translation="MRADRGLAELATSACATEQALKRAQALVGKELEHADLKPVISAC
                     APPSGLAAENLSRAAAAPADVVSAWLGSPGHANNLLSPDLTQLGVGCVRAVDEPNPQL
                     LCAQVFLG"
     gene            3663..3760
                     /gene="ffs"
                     /locus_tag="PKJ61_10370"
     ncRNA           3663..3760
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PKJ61_10370"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            4099..>5586
                     /locus_tag="PKJ61_10375"
     CDS             4099..>5586
                     /locus_tag="PKJ61_10375"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020574906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma and tau"
                     /protein_id="HOC14267.1"
                     /translation="MEVVDALRQPSQAVSRPDTPLALYRRYRPDTFADVIGQEHVTEP
                     LQRALVNNRVNHAYLFSGPRGCGKTTSARILARALNCAEGPTPVPCGKCQSCRDLARG
                     GPGSIDVIEIDAASHGGVDDARDLRERAFFAPVHSRYKIYIIDEAHMVTPQGFNALLK
                     VVEEPPPHVRFVFATTEPDKVLGTIRSRTHHYPFRLVPPKTLSAYLATLCEAEGIAVE
                     PAVLPLVVRAGAGSVRDSLSVLDQLLGGAGEDGVSYPQATALLGYTPDALLDEIMDAF
                     AAGDSRGVFAAVDKVIEVGQDPRRFAEDLLRRLRDLIIVAAVPEALSSGLVDVAADQG
                     ERLHIQANAMGAGELTRAAEVIAVGLTEMRGTTAPRLHLELMCARVLLPGADVDGRGL
                     HARLDRLERRLSMGTVPEEPASRRAPDAGSRQARPAEESQPAREEPARTEPASRSAVL
                     RSPASDEPGVTKGAAAAPAPADIPAPADIPAPAVIPAKAGIPKRDE"
     assembly_gap    5587..5686
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5687..6470
                     /locus_tag="PKJ61_10380"
     CDS             <5687..6470
                     /locus_tag="PKJ61_10380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14268.1"
                     /translation="IPAPAVIPAKAGIPKRDEPVDTPPWIDAEMVPVEPREPASEASR
                     SAVLRSPASDEPGVTKEAADAGYPPARPAESARPAETPGVLGTTDVRRFWPQILDNVK
                     NRRRFTWILLSQNAQVVEVREGVLTLGLNNAGAREGFARGGNADVLREAILEVLGAAL
                     HIDPIVDANATPSPASSPAREAAPGPSVPPSSPASPPRSAAAEAARANLRPTRSAAAI
                     AAEDPEPEPNRDDEVVDESSTSSSEDLLMKHLGAELVAEDEN"
     gene            6518..6829
                     /locus_tag="PKJ61_10385"
     CDS             6518..6829
                     /locus_tag="PKJ61_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002518668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HOC14269.1"
                     /translation="MFDPSGFDLQGLLSQAQAMQDQLEAAQAELANRRVTGSAGGDLV
                     EATLTGTGELVGLVIKPEACDPDDTETLADLIVAAVRDANHQAMALAQAAMPPMPELD
                     L"
     gene            6967..7581
                     /gene="recR"
                     /locus_tag="PKJ61_10390"
     CDS             6967..7581
                     /gene="recR"
                     /locus_tag="PKJ61_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015069359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOC14270.1"
                     /translation="MYEGPIQDLIDELGRLPGIGPKGAQRIAFHILQTDKADVERLAN
                     VLREVTEKVRFCDICFNISEEERCRVCRDPRRDRTAICVVEESKDVMAIERTHEFRGL
                     YHVLGGAISPIDNVGPGDLHIRELLTRLQDETVTEVILATDPNLEGEATATFIARLLN
                     GTGVTVSRLASGLPVGGDLEYADEVTLGRAFSGRRRLNQESASL"
     gene            7578..8924
                     /locus_tag="PKJ61_10395"
     CDS             7578..8924
                     /locus_tag="PKJ61_10395"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HOC14271.1"
                     /translation="MNHALVRLIASDLDGTFLRDDRTVSGRTTGAVRAARASGVMVIA
                     ATGRQAQQLPAEVAASGIRHVVASNGAIGFDLVEQRVLFQDLLAPAAIAAIVSYLKER
                     LPDVRFSAVRDHGARHAAEPGYVDLLADAERGAWWRIDTEPLADVVSEPTLKLTVRHP
                     SLTADQLLSVLSESGMVGFHATTSGAPFLEIAGAGVTKASGVAQLCRLLEVEAAEVLA
                     AGDAKNDIELLTWAGVGVAMGNAVPEARAAADWVTATNEEDGLAMAIEAVLTARASGG
                     SMTEPALNPTEDETLMTDEPVLGDPVLSAWAAACVDHALLQFGDLVPADATTALESVR
                     AWTAGAGSAEASRDAAFAAHLAARDLQDAGYQAAALAVRAASNAAASVDDPTLALQSA
                     AQALEAFTLNSAPCEVQFNAESERRWQWTQLPLSHRARVFPTEPGEPAAASCALPS"
BASE COUNT         1504 a         3209 c         2949 g         1288 t
ORIGIN      
        1 ttgtcgaagc ccgctacgac aagatgcacc tcttcgatgc ccttggctac gccgagtcac
       61 gacagatcca gcccggcaca cagccggtcc acttcgatct ggccgggacg cggatcgggc
      121 tggccacctg ctacgacgtc cgcttcccac agttgttcac ccacctggcc ggcaccggag
      181 ctgaactgat cctgctgccc gcatcctggg cgcccggcga gcacaaactg catcagtggc
      241 ggaccctggt cacagcccga gcaatggacg ccaccgcctt cgtagtcgcg gtcgaccagg
      301 ccctctcccc cgccgcagcc gacggacgga cgcccaccgg catcggccac tcgatggtgg
      361 ttgatccaac cggcgacgtg ctcctcgaac tcggcgacga cccggagttc gccgtcatcg
      421 acctgaacct cgcccaagtc gccgaggtgc gccgccggct gcccgtcctg gagcacacgc
      481 ggcgcttcaa ctgagcgtcg tcgggatctc gacaggttcg acgagccgat gggtacgctg
      541 acagtgtcac gccacgctgc agtcggggga acgcatgtcg gccgatgatc tgcctgccga
      601 ggtcctgaac aacgggcccc gccagccggg cgtgggaatg gcagtgagaa agcgtcgctg
      661 gccgtggatc gcactcactg cggtcgtgct caccgtcgcc gccgtgggca tttctggctt
      721 cctcgtctac caggacgcca tgcgcgcccg cgactcccta caggtagccg cggacgccct
      781 gccgcaggtg gcgacacaga tccgcagcga cccgcagcgg gcggacgcca ccatcgcccg
      841 ggtacagatg gccgcccaca gcgcactgga agccaccacc ggcccgcatt ggaccgtcgc
      901 cggctggctg ccctgggtcg gcgacaacac ccgcgcgctg cagaccgtga ccacggccgt
      961 cgaccggatc gcgtccggcg tgcttcccga gctggccgca tccgtgcaga gtgtcacgcc
     1021 cgaacgcctc gcaccgcagg acggcaagct caacctcgca ccactggtcg aggtgcgcga
     1081 gcgcgtagtg gacgccgacc agatcgttgg ccaggcgatc actgacgtgt cgggcattcc
     1141 cagcgccaac ctgatcgacc agctcgcgga cgcggtcgcg cgcctccagc aggaactcac
     1201 cgacgcccgc gcactgacgt ccaccgcgtc cgccgcggtt cagctgctgc ccgcgatgct
     1261 cggcgcggac ggaccccgcg actggctcct gctcgcccaa aacaatgctg agtcgcgcgc
     1321 caccggcggc atcccaggcg ccgcgattct gctgcacgcc gacaacggca ggctcagcat
     1381 cgtcaaacaa ctcggggccg gcgatttcgg gccttaccca gagcccatcc tgccgctgac
     1441 cgccgacgag agccggctgt tcggcgccgc cctgggccac tacctgcagg acgtgaacct
     1501 gacgcccgac tttccacgca gcggccaact cgtgcgggcg atctgggcgg cggacggtgg
     1561 cgccagcgat gtcgccaacg tggtctcgat cgatccggta gcactacaga ccatcctgcg
     1621 cgccaccggg ccggtccaat tcaccgaccc ccggggccat gccatcaccc tcgacgccga
     1681 caacacggcc agcttcctga tgtctgaggt ctaccgtagg ttcgactcac ccgacgcgca
     1741 ggacgcggtt ttcgcacttg ctgtgcaggc agtgcttcag caggtgatgt cgggcgaggc
     1801 ggacgccggt gagctgcttg gtgccgtgac cgaaatcgcc ggtaacggcc ggctgctggt
     1861 ctggtccgcg gacgcgtccg aacagcagca gctggagacg atcggcgtca ccggcgcgct
     1921 gcggggatcg atccaagccg gcgaaggcac gagaccggtg gtaggtgtct tcctcaatct
     1981 gaccaccgcc tccaagctcg gctactacct cgacctgaag gccactatcg gcgaagtcaa
     2041 ggcgcgtccg gacgggtcgc gggagttcga cgtgactgtg cggctgaagt cccgactcac
     2101 ccctgcggag gctcgacggc tgccgtccca tgtgattgaa aatgccccca gggacgggac
     2161 caatcgcgtg aacgtgctgg tctatgcgcc caccgatggc acgatcacgc agctgagtac
     2221 cgcatcgccg ggcttcgtga ccacccacga cggcctgcag gtcagcgcgc agacggtgac
     2281 ggttccgcct gaaagcgcgg ccgaggtccg attccacatc gtcaccggtc ccggtcagga
     2341 cgccgagccg tacctgcgcc agacacctgg cgcgcgcaac gcttgagaca ccctttctga
     2401 acccgctctg ggcaaagaaa aagactgcag atcaattgct tacttcgcca ccgagccgcg
     2461 ggtgtaggct gaagtcgtcg gccagagtcg ggggacgttg gccaacgatt tgcagagttc
     2521 tgaagcttgg ggggagcacc atgaatctgc gcgcactcgg cacatccatc gcggccacag
     2581 cactcgtctt cgcattcccg gcagccgcct acgccgaaac tgcgagcccg gatcccggtc
     2641 cagaaacgac cgtgccgaac tacgaggcca gggagtactg caagatcacc gcgaacccga
     2701 ccagcctgca ggccggcggc actactcaga tcagtctggt cgacgctccg aacgccgaaa
     2761 ccgacctgag cgtgacttca gcggacgctt cggtcaagga cagcgccatc cagatcgccg
     2821 gcaagcagac cgcagtgaag aaggcggacg cgaacggcaa cgtctccttc tcagtgacgc
     2881 tcgccgaacc gggtgactat atcctcgacg cgtccacgat caccggtcag ttctgcggcc
     2941 tcaccgtcaa ggtttcgccc aaggaaaagg tcgtggaggc tgctgaggac gaggacgaaa
     3001 ccccggtcgc caacgccgcc gcagcagtgc tcgccaacac cggcgccacg acgttgccct
     3061 acgtgggcgc agcggtcgtg ctgctcggtg caggtgcggt ggcagtggtc gcgacccggc
     3121 gtcgccggaa cagctgagcc gagccagaaa cgtccggctg gtcgccacgc ccatccggcg
     3181 ggtcgaacct gtcgagatca ctgctcgtag tcctgttgct cctggccggc tgcaccgcgc
     3241 ccccgtccag cccttcgtca acgagccctg cggatcttga cccagcagag tacgcctacg
     3301 ccatcaccac cgagacgaat cgggtgcgcg cagaccgcgg gctggccgaa ctcgcgacgt
     3361 ccgcttgtgc aactgagcaa gcactgaagc gcgcgcaggc cctggtcggc aaggagctcg
     3421 aacacgccga cctgaagccg gtgatcagcg cgtgcgcgcc cccgtccggc ctggctgcgg
     3481 agaacctcag ccgggctgcg gcagcgcccg cagacgtggt cagcgcctgg ctgggctccc
     3541 cggggcacgc gaacaacctc ctcagcccgg acctgaccca gctcggtgtg ggctgcgtcc
     3601 gcgctgtcga cgaaccgaat ccgcagctgc tgtgtgcaca ggtattcctc gggtaggctt
     3661 acccttggtt ccccgcgcgg cggtacctcg cccaactccc ccagggtcgg aagacagcaa
     3721 gggtaagtga gctcttccgg gtgcgcgggg gccgcttcca attccggggc cgtctcaatt
     3781 ccagggccgt tcaactccat ggtcgctgcg aattccggcc cggcagaagt tctgtcactg
     3841 gcaccccgta aggttcaacc cgtggacgag cgtgacgatg acctgttcga agaagagccg
     3901 ctggagctct tcgagcagga cggtcctgac ctattcggcg ggttcgacga acccgtggcc
     3961 gacaaggcag acgagcctgg cgtcgaggac ctccccaccg aagagcctcc ggtcgaagac
     4021 ctgccgctga acgccggcag ccagccggta gaggtgggcc cgcatcgccg ctctgaggaa
     4081 gaggtcaccg cttcattgat ggaggtggtc gacgccttgc gccagccgtc ccaggcggtg
     4141 tcgcgtccgg acactccgct ggcgctgtac cgccgctatc gtccggacac cttcgcggac
     4201 gtgatcggcc aggagcacgt caccgagccg ctgcagcgag cactggtcaa caaccgggtg
     4261 aatcacgcct atctgttctc cggtccgcgc gggtgtggca agaccaccag cgcccgtatt
     4321 ctggcgcgcg cgctgaactg tgccgaaggg ccgacgccgg tgccgtgcgg caagtgccag
     4381 tcctgccgcg atctggccag gggcggaccg ggcagtattg acgtgatcga gatcgacgcc
     4441 gcatcgcacg gcggtgtgga cgatgcccgc gacctgcgcg aacgggcgtt cttcgcaccg
     4501 gtccacagcc gctacaagat ctacatcatc gacgaggccc acatggtcac gccgcagggt
     4561 ttcaacgccc tgctcaaggt ggtggaggag ccgccgccac acgtccgctt cgtttttgcg
     4621 acgaccgagc ccgacaaggt gctgggcacg atccgttcgc ggacgcacca ctacccgttc
     4681 cggctggtgc cgccgaagac gctgtcggcc tatctggcca cgctgtgtga ggccgagggc
     4741 atcgcggtcg agccggcagt gctgccgctg gtggtacgcg ccggcgccgg ttcggtgcgc
     4801 gactcgctgt cggtgcttga ccaactgctc ggcggcgccg gcgaggacgg ggtcagctac
     4861 ccgcaggcca ccgcgctgct gggctacacc ccggacgcgc tgctcgacga gatcatggac
     4921 gcgttcgcgg ccggcgactc tcgcggcgtc ttcgctgccg tcgacaaggt gatcgaggtg
     4981 ggtcaggatc cacgtcgctt cgccgaggac ctgctgcgcc gtctgcgtga cctgatcatc
     5041 gtggccgcgg tgccggaggc gctgtccagc ggcctggtgg acgtcgctgc cgaccagggc
     5101 gagcgcctgc acattcaggc caacgcgatg ggcgccggcg agctcacccg ggccgcagag
     5161 gtgatcgcgg tcgggctcac cgaaatgcgc ggcaccaccg ccccgcgatt gcacctggaa
     5221 ctgatgtgcg cccgcgtcct gctgcccggg gctgacgtcg acggccgcgg cctgcacgcc
     5281 cgcctggacc gcctggagcg ccgcctgagc atgggcacgg tcccggaaga gccggcatca
     5341 aggagagcgc cggacgcagg gtctcggcaa gctcgaccag ccgaggaatc acaaccggcc
     5401 cgtgaggagc cggcgagaac cgagccggcg tcacgaagcg ccgtcctgag gagcccggcc
     5461 agcgacgagc cgggcgtcac gaagggcgcc gccgccgctc cggcacccgc cgacatcccg
     5521 gcacccgccg acatcccggc acccgccgtc atcccggcga aggccgggat ccccaagcgc
     5581 gacgagnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncatc ccggcacccg
     5701 ccgtcatccc ggcgaaggcc gggatcccca agcgcgacga gccggtcgat accccgcctt
     5761 ggatcgacgc cgaaatggtc cccgtggagc ctcgcgaacc tgcgagcgag gcatcacgaa
     5821 gcgccgtcct gaggagcccg gccagcgacg agccgggcgt cacgaaggag gccgccgacg
     5881 ctgggtatcc accggctcga ccagccgagt cagctcgacc agccgagact ccgggcgtcc
     5941 tgggcaccac cgacgtccgc aggttctggc cgcagatcct tgacaacgtg aagaatcgcc
     6001 gtcgcttcac ctggatcctg ctcagccaga acgcgcaggt cgtcgaggtg cgcgagggcg
     6061 tcctgaccct cggcctgaac aacgccggcg cgcgcgaagg cttcgcccgc ggcggcaacg
     6121 cggacgtgct gcgcgaggcg atcctcgaag ttctcggcgc agccctgcac atcgatccga
     6181 tcgtggacgc caacgcgacc ccgtccccgg cgagcagccc cgcccgtgaa gccgcgccgg
     6241 gcccgagcgt tccgccgagt tcaccggcca gcccaccccg ttcggcagcc gccgaggccg
     6301 cgcgcgcgaa cctgcgtccg acccggagcg cggccgcgat cgcggccgag gatccggaac
     6361 cggagccgaa ccgcgacgac gaggtggtcg atgagtcgag cacgtcctcg tcggaggatc
     6421 tactgatgaa acacctcggc gccgagctgg tggctgagga cgagaactga accgagggcg
     6481 cggctggcac gccggcgtca gagatggagg agacaccatg ttcgacccga gtgggttcga
     6541 cctgcaggga ctgctctcgc aggcccaggc catgcaggac cagcttgagg ccgcccaggc
     6601 cgagctcgcc aaccggcggg tgactggcag cgccggcggc gacctcgtcg aggcgaccct
     6661 caccggcacc ggggaactgg tcggcctggt gatcaagccc gaggcctgcg atccggatga
     6721 caccgagacg ctggccgacc tgatcgtggc cgcggtcagg gacgccaacc accaggcaat
     6781 ggcgcttgca caagcggcga tgccgccgat gcccgagctc gacctgtaat cactcaccgg
     6841 ctggccgagc cattcggctg atcgagcctg tcgaggccac gcccaacgct ggtcgagcct
     6901 gtcgagacca ccactcgtcg gcccgccgag cttgtcgaga ccaatcgcac tgactaagga
     6961 accaccgtgt acgaaggccc gatccaggac ctgatcgacg aactgggccg gcttcccggc
     7021 atcgggccga agggcgcgca gcggatcgcg ttccacatcc tgcagaccga caaggccgac
     7081 gtcgaacgcc tggccaacgt gctgcgcgag gtcaccgaga aggtgcggtt ctgcgacatc
     7141 tgcttcaaca tctccgagga agagcgctgc cgggtctgcc gggatccgcg ccgcgaccgc
     7201 acggccatct gcgtggtcga ggagtcgaag gacgtgatgg cgatcgagcg gacgcatgag
     7261 ttccgcggcc tgtaccacgt gctcggcggc gcgatcagtc cgatcgataa cgtcggtccg
     7321 ggcgacctgc acatccgcga gttgctcacc cggttgcagg acgagaccgt caccgaggtg
     7381 atcctggcga ccgacccgaa cctcgagggc gaggcaacgg ccaccttcat cgcccggctg
     7441 ctgaacggca ccggcgtaac cgtttcccgt cttgccagcg gcttgccggt cggtggcgac
     7501 cttgagtacg ccgacgaggt cacgctcggc cgcgccttct ccggacgccg ccggctcaac
     7561 caggagagcg cgtcgctgtg aatcacgccc tcgtccgcct gatcgccagc gatctggacg
     7621 gcaccttcct gcgcgacgat cgcaccgtca gcggacgcac gaccggcgcg gtgcgtgccg
     7681 ccagagcgtc cggggtgatg gtgatcgcag ccaccggccg ccaggcccag cagctgccag
     7741 ctgaggtggc tgccagcggc atccggcacg tcgtggccag caatggcgcg atcggcttcg
     7801 atctggtgga gcagcgggtg ctcttccagg acctgctcgc cccggccgcg atcgccgcga
     7861 tcgtgagcta cctgaaggag cggctcccgg acgtccgctt ctccgccgtg cgcgaccacg
     7921 gcgcacggca tgcggccgaa cccggctacg tggacctgct cgcggacgcc gagcgcggcg
     7981 cgtggtggcg gatcgacacc gagccactgg cggacgtggt cagcgagccg accctcaagc
     8041 tgactgtccg gcatccatcc ttgaccgccg accagctgct gagcgtgctg tccgagtcgg
     8101 gcatggtcgg cttccacgcc accacttctg gcgcgccgtt cctcgagatc gcgggcgccg
     8161 gtgtcacgaa ggcgagcggt gtcgcccagc tctgtcgcct gctggaggta gaggcagcgg
     8221 aggtgctcgc cgccggcgac gccaagaacg acatcgaact gctgacctgg gccggtgtgg
     8281 gcgtggccat gggcaacgcg gttccggaag cacgcgccgc cgcggactgg gtaactgcca
     8341 ccaatgaaga ggacggcctg gcgatggcga tcgaagccgt cctcaccgcc cgagcttctg
     8401 gagggagcat gaccgagccc gcgctgaacc cgaccgagga cgagaccctg atgactgacg
     8461 aacctgtgct gggcgacccc gtcctgtccg cctgggcggc ggcctgtgtc gaccacgccc
     8521 tgctccagtt cggcgacctc gtcccggcgg acgcaacgac cgcgctcgag tccgtccgcg
     8581 cttggacggc aggcgccggt tcggccgagg ccagccgtga cgccgccttt gccgcgcacc
     8641 tcgccgctcg cgacctgcag gacgccggct atcaggccgc tgcgctagcg gtccgcgcag
     8701 ccagcaatgc ggccgcgagc gtggacgatc ccacgctggc tttgcagtcc gccgcccagg
     8761 cgctcgaagc attcaccctg aacagcgccc cctgcgaggt gcaattcaac gccgagtccg
     8821 aacgccgctg gcagtggacg cagctccccc tcagccaccg cgcccgcgtc ttcccaaccg
     8881 agcccggaga gcctgccgcc gcctcctgcg ccctcccgtc gtagcggtcc ccgccacccc
     8941 gcaacggtcc ctgaggtcct cagggaccga ctgcgggcaa ggtcttgcgg gattcacagg
     9001 tccagcacag gtgggcgagt aagcatggac ttcgtcggcg tcaccgggac
//
LOCUS       DAOPRQ010000088         8774 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_6661_length_8774_cov_12.316894, whole genome shotgun sequence.
ACCESSION   DAOPRQ010000088 DAOPRQ010000000
VERSION     DAOPRQ010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 8774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8774
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6661_length_8774_cov_12.316894"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1317
                     /locus_tag="PKJ61_10400"
     CDS             <1..1317
                     /locus_tag="PKJ61_10400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14272.1"
                     /translation="SQGRASADAGLAAVLAAFKKNSATCPSPTPSRTATPRYSTTCVE
                     SSGQVTFTSTGDPGLATAKKVEAVYVVNTSSTAGGAAELVFFNTGSDSVYFTNHVLPQ
                     SSTLSTILFPGGGTFECKTTVPGNIIMAGSFLGQSGCIVNGSVWAGGENPAQGKWALY
                     LNNDDQVLGSATAVGKVAIGGGSSKIGGTLTLPSSAELQIAWMNAGKPTSNARVSSGA
                     SGAISWPASVAKPTLAGWFEYTHSDAQWVGYAPVKITASSTPYNCSNINDWSTTFWSS
                     YVTGLTQDTVIDMTACSDGIKQVKNGKATIGVNVALIGNRFILSDFALAPKAGKDPRA
                     FIVVPDPNSSDNKPTCSGSRTIETNAAVNVTVKTMAYTPCTIRVGYGGVWTGAMYSGT
                     LDDGGDIKIYTSPMGLPGQFNAVTGGASGAGTKSLGDLVSRRDVNP"
     gene            1314..2132
                     /locus_tag="PKJ61_10405"
     CDS             1314..2132
                     /locus_tag="PKJ61_10405"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HOC14273.1"
                     /translation="MSVSSVFLITVAVIFGTVIGSFLNVVAYRVPAGISLMRESRCPG
                     CGSPVQWYHNIPVFSWFTLLGRCAACDARISFRYPVVEAATGLLFGLVAWFVPLAMPL
                     PGFAGFAVLLAFWWFAAITVVLVLIDLDTKRLPDVIVLPSYAVGGVLLLLACLFGADW
                     GALLRAGIGLGALYGFYALLRLVRPGGMGGGDVKLAGVAGMYLGWLGWGPLMVGGFAA
                     FLLGGVFGIALLASGRAQRRTAIPFGPWLLAGAWVGVLAGVRLGAWYGGLYGLY"
     gene            2201..3199
                     /gene="pilM"
                     /locus_tag="PKJ61_10410"
     CDS             2201..3199
                     /gene="pilM"
                     /locus_tag="PKJ61_10410"
                     /inference="COORDINATES: protein motif:HMM:NF022554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HOC14274.1"
                     /translation="MASVIGVEIAEQSIRAVEISRGRRPSVIAAGEVPLPEGVAKDSE
                     ILDPDAASLALRQLWSQANLRGGRVVLGISNRRVLVREYVSPRLAPELLRAALPYEVQ
                     DLLPVPVDQAVLDFYAVSETDTHILGLLVAAVSETVEGLIQTLANAKIRTEAVDFIPF
                     GLARVVRSLRGVEPVVAIASVGEHTTNVVVTSEGIPRFVRIIPADLTTGLLAPAGAVA
                     EAPGNPDGWVARRALATLPVETADPAITDLVGRLRGTLRFYAGREGAEPIHRLFLTGS
                     GLSMPGVAAAVASGLELPVTELGVTDVIGLSKSVAAQGAPPPSLMNALGIALGDEK"
     gene            3196..3864
                     /locus_tag="PKJ61_10415"
     CDS             3196..3864
                     /locus_tag="PKJ61_10415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14275.1"
                     /translation="MNLNIRPTKLPPAQAGKAPRVNLMPASEVARRERRVLLRNWMLG
                     LFATLAGLGLGVGAMLGVRMGAELRLAEENQRTEQLTIALAGYADVTKSLADRSALGD
                     FRGSAAANDLPWTELYGDISGALPGGVTVRGFDLHPGAAPMADADPATAAGLTGTITC
                     RSTDPGDQRRMVTALRKIPGALAVDAGKLDYLETDATYEYVITFVADQTRYTGRFAPE
                     GGVK"
     gene            3861..4478
                     /locus_tag="PKJ61_10420"
     CDS             3861..4478
                     /locus_tag="PKJ61_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14276.1"
                     /translation="MNLPKRLINLIGTVLVVGMLAVGIFMVAWPLYTGHLEVEAQRLS
                     VAQSNDVQAARIQELAKQQAELPALTAELDGLRQQIPVTASLDQASKLAVAAAKATGS
                     HLQTFAVGDTVAFTPPAGVVTDAAAAAPAPAPTDGSAAESPRLQTSVSFTITTDSTAQ
                     VAEFVDKLRTGSRLVQIVQVLVSTGDEVPVVVTGTVTALIFVQQP"
     gene            4488..5630
                     /locus_tag="PKJ61_10425"
     CDS             4488..5630
                     /locus_tag="PKJ61_10425"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HOC14277.1"
                     /translation="MPELTALDRLLATAVQARASDVHLSADHPPLMRLDGALIGVPGY
                     SDQLSGAWLETALLAIMGPALRDLYKVESEVDLAHSVPGIGRFRVNVFHQIGGVAAAL
                     RLVPEQVKTLAELGIPPVARELALRPRGLVLVTGPTGSGKSTTLAAMVDVVNREVPAH
                     IITVEDPIEYIHTSKRSLIHQREIGSDTPSFADALRHILRQDPDVVLIGELRDPESIS
                     IALTAAETGQLVLGTLHTQGAAKTIDRIIDTFPARQQSQVRSQLGDTLQGVVSQTLLP
                     RAQSGGRVLATEVLVHTPAVANLIREGQVQQLYSVMQSSSPLGMHTLDQSLVELVASG
                     TVSRQVAQGFVVDPRALDNVRVRPQDLDADAWSQHAAMQQRARVGA"
     gene            5630..6865
                     /locus_tag="PKJ61_10430"
     CDS             5630..6865
                     /locus_tag="PKJ61_10430"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOC14278.1"
                     /translation="MALMEEFTYQAVSPTGGPVVKGTLEASGANAVALKLRAQGLMPL
                     KIQAVSKTGLNRDVKLFNREGRVKVKPLALFAKQMAGLINAGLPLMRSLSVLIEQTED
                     ASLRSALTAVHADLETGHSFSAALAKQPHAFPPLMVNLVKVGETGGFMGDSLELAAKS
                     YGADAELQDKLKAASTYPMVVLVIAVLAVIGMIAFIVPIFEGMFASMGGELPLPTQIL
                     VTMSHNMVWLLPLLIVAVVAFWFWWMRNRNTEAVRKVIDPYKLKMPIFGTLMTKVAVA
                     RFSRNLSMMLAAGVPLLQALSTVGKASNNWAIEQAVLDVQKSVREGRSFAAPLAKAAV
                     FPPIVAQMVSVGEESGTLPDMLASIADMYEAEAKTATDQLASTIEPILIVMIGLLIGS
                     MVLALYMPIFGIYSQLAEQ"
     gene            7046..7294
                     /locus_tag="PKJ61_10435"
     CDS             7046..7294
                     /locus_tag="PKJ61_10435"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HOC14279.1"
                     /translation="MKTITVGQLRQNPTAMLADVEAGETYRVTRHNREVARLVPRGRE
                     LELLPRKRAAGSRLSELPRHELSTAATIDELLAEERDR"
     gene            7291..7692
                     /locus_tag="PKJ61_10440"
     CDS             7291..7692
                     /locus_tag="PKJ61_10440"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HOC14280.1"
                     /translation="MTTFYLDTSVALRALLGHSPSAAKWIDQVTADDEHLLVSSRMLR
                     TELTRILRREGLPVNRRDEVLDVLSLVPITEGVLAAAEAIQPHVKTLDAIHLGSVIAA
                     GLDATIVSHDATMIATATSLGYPTFDPVTDR"
     gene            complement(7878..>8774)
                     /locus_tag="PKJ61_10445"
     CDS             complement(7878..>8774)
                     /locus_tag="PKJ61_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14281.1"
                     /translation="AGFTRAMIETQLRAGRLVRLRAGVFLAASALPAEPVGHHLILAH
                     AEQTRYPEAVLSHESAAIVWNLPIPGVRPWHESPAAITFPAGTTQRSRHGVANHHIGK
                     LPPHHLGRDAAGYIVTSVARTAVDLAGRHPLPEQLVLLDAAARRICESLVANPRRRDY
                     ANPKLIAEARRLLLEAATVIRSGRLRLGISLCTPERESAAESLSAGHLHLAGIPTPLF
                     NPPIHTAKGTLYPDCYWPDHNLIGECDGKVKYRDPEAAVREKEREQVFRDDGYRMVRW
                     LAKEAMFAPSVMTTRVWRKLDE"
BASE COUNT         1389 a         2959 c         2930 g         1496 t
ORIGIN      
        1 tcccaggggc gcgcgtccgc cgacgccggt ctggccgcgg tgctcgctgc attcaagaag
       61 aacagtgcca cctgcccgtc accgacccct tcacgaaccg cgacgccgcg gtactcgacc
      121 acctgcgtgg agtcgtccgg ccaggtgacc ttcacatcca ccggcgatcc cgggctggcg
      181 accgcgaaga aggtggaagc ggtctacgtc gtcaacacat ccagcaccgc cggcggcgca
      241 gccgagctgg tgttcttcaa caccggatcc gactcggtct acttcaccaa ccacgtcctg
      301 ccgcagtcct cgactctttc cacaatcctg ttcccgggcg gtggcacctt cgagtgcaag
      361 accaccgtcc ccggcaacat catcatggcc ggctcgttcc tcgggcagtc cggctgcatc
      421 gtcaacggca gcgtctgggc cggcggcgag aaccccgccc agggcaagtg ggcgctgtac
      481 ctgaacaacg acgaccaggt gctcggctcg gcgactgctg tcggcaaggt cgccatcggc
      541 ggtggcagtt ccaagatcgg cggcaccctc accctgccct cgagcgccga acttcagatc
      601 gcctggatga acgccgggaa gccgaccagc aatgcccggg tctccagcgg cgcgtccggc
      661 gcgatcagct ggcccgcctc ggtcgctaag cccaccctcg ccggctggtt cgagtacacc
      721 cacagcgacg cccaatgggt gggctacgca ccggtcaaga tcaccgccag ctcaacgccg
      781 tacaactgct cgaacatcaa cgactggtcg accacgttct ggtcgagcta cgtcaccggg
      841 ctcacccagg acacggtcat cgacatgacc gcctgctcgg acggcatcaa gcaggtgaag
      901 aacggcaaag ccaccatcgg cgtcaacgtg gcgttgatcg gcaaccgctt catcctcagc
      961 gacttcgcgc tcgcccccaa ggcaggcaag gacccgcggg ccttcatcgt cgtgcccgac
     1021 ccgaacagtt ccgacaacaa gcccacctgc tccggcagcc gcaccattga gacgaacgcc
     1081 gcagtgaacg tgaccgtcaa gaccatggcc tacaccccgt gcacgatccg ggtcggctac
     1141 ggcggcgtct ggacaggcgc tatgtactcc ggcaccctcg acgacggcgg cgacatcaag
     1201 atctacacct cgccgatggg cctgcccggc cagttcaacg cggtcaccgg cggcgcgtcc
     1261 ggtgccggca ccaagtcgct cggtgacctg gtcagccgac gggacgtgaa cccgtgagcg
     1321 tttccagcgt cttcctgatc acagtggccg tcatcttcgg cacggtcatc gggtcgttcc
     1381 tgaacgtggt cgcctaccgt gtgccggcgg gtatctcgct gatgcgggag agccgctgtc
     1441 ctggatgtgg ctcgccggtg cagtggtacc acaacatccc ggtcttctcc tggttcaccc
     1501 tgctcggacg ctgcgccgcg tgcgatgcgc ggatctcctt ccgctacccg gtggtcgagg
     1561 cggccaccgg actgctgttc gggctcgtgg cctggttcgt cccgttggcc atgccgctgc
     1621 cgggcttcgc tggtttcgcc gtgctgctcg ccttctggtg gttcgcagcc atcaccgtgg
     1681 tgctggtgct gatcgacctc gacaccaagc gattgccaga cgtgatcgtg ctgcccagct
     1741 atgcagtcgg cggcgtcctg ctccttcttg cctgcctgtt cggtgcggac tggggtgcac
     1801 tgctgcgcgc cggcatcggt ttgggtgcgc tctatggctt ctatgcgctg ctgcgcctcg
     1861 tccgccctgg cggcatgggt ggcggggacg tcaagctcgc cggagtggcc ggcatgtacc
     1921 tcggctggct cggctggggg ccgctaatgg ttgggggctt tgctgccttt ctcctcggcg
     1981 gagtgttcgg catcgccctg ctcgcgtccg gacgcgccca gcgacgcacc gcgattccgt
     2041 tcggcccgtg gctgctggcc ggcgcctggg tgggcgtgct ggccggggtc cggttgggtg
     2101 cgtggtacgg gggactctat gggctctatt gaggtccggg gcgatggtct cgacaagttc
     2161 gaccagacga gtggtgcggc cgacaagcga agggtgaggc atggctagcg tgattggcgt
     2221 cgagatcgcc gaacagagca ttcgcgcggt cgagatcagc cgcggccggc gccccagcgt
     2281 catcgccgcc ggcgaagtgc ctctgcccga aggcgtggcc aaggactctg agatcctcga
     2341 tccggacgcg gcctcgctgg cgttgcggca gctctggtcg caggccaatc tgcgcggcgg
     2401 ccgggtggtg ctgggcattt ccaaccggcg ggtgctggtg cgcgagtacg tgtctccccg
     2461 gctcgcgccc gaactcctca gggcggcgct gccctacgag gtgcaggatc tcctgcccgt
     2521 cccggtcgac caggctgtgc tcgacttcta cgcggtcagc gagaccgaca cccacattct
     2581 gggactgctg gtcgctgcgg tatccgagac cgtggagggc ctcatccaga cgctcgccaa
     2641 cgccaagatc cgcaccgaag ccgtcgactt cattcccttc gggctggccc gggtggtgcg
     2701 cagcctgcgc ggcgtcgaac cggtcgtggc gatcgcgtcc gtcggtgagc acacgaccaa
     2761 tgtggtggtg accagcgagg gcatcccgcg cttcgtgcgg atcatcccgg ccgacctcac
     2821 caccggcctg ctcgcgcccg cgggtgccgt cgccgaggcg cccggcaacc cggacggatg
     2881 ggtcgcccgc cgagccctcg ctacgctccc ggtggaaacc gccgacccag cgatcaccga
     2941 cctggtcgga cggctgcgcg gcacgctgcg gttctacgcc ggacgcgaag gcgccgaacc
     3001 gatccaccgg ctgttcctga ccggctcggg actgtcgatg ccaggggtcg cggctgcggt
     3061 cgcgtccggg ctcgaactgc cggttaccga gcttggcgtc accgacgtga tcgggctgtc
     3121 caagtcagtg gccgcgcagg gtgcgccccc gccgagcctg atgaacgctc tcggcatcgc
     3181 gttgggagac gagaaatgaa cctcaacatt cgtccgacta agttgccgcc cgcacaggcc
     3241 ggcaaggcgc cccgggtcaa cctcatgccg gccagcgaag tggccaggcg tgagcggcgc
     3301 gtcctcctgc gcaactggat gctcggcctc ttcgccaccc tggctggtct ggggctcggc
     3361 gtcggcgcga tgctcggcgt ccgcatgggc gcggagcttc gccttgccga ggagaatcag
     3421 cgcaccgagc agctcaccat cgccctggcc gggtacgccg acgtgaccaa gagcctcgcc
     3481 gaccgctcag ccctgggcga cttccgcggc tcggccgcgg ccaatgacct gccctggacc
     3541 gagctctacg gcgacatctc cggtgcccta cccggcggag tgaccgtgcg gggcttcgac
     3601 ctgcacccag gcgcggcgcc gatggcggac gccgatccgg ccaccgcggc cggcctgacc
     3661 ggaaccatca cctgccgcag caccgacccg ggtgaccagc ggcggatggt gaccgcacta
     3721 cgaaagatcc cgggggcttt ggcggtggac gcgggcaagc tcgactatct ggagaccgac
     3781 gccacctacg agtacgtgat caccttcgtc gccgaccaga cccgctacac cggacgcttc
     3841 gctcccgagg gcggtgtgaa atgaatctgc ccaagcgcct gatcaacctg atcggcaccg
     3901 tactggtggt cggaatgctg gccgtcggca ttttcatggt ggcctggccg ctgtacaccg
     3961 gccacctaga ggtggaggcg cagcgactgt ccgtggccca gtccaacgac gtgcaggcgg
     4021 cccggatcca ggaactggcc aagcagcagg ccgaactgcc ggccctcacc gccgaactcg
     4081 acggcctgcg tcagcagatc ccggtcaccg cgtcgctcga ccaggcgtcc aagctggcgg
     4141 tcgcggccgc caaagccacc ggttcgcacc tgcagacctt cgcggtcggc gacactgttg
     4201 cgttcactcc gcccgccgga gtggtcaccg atgccgccgc ggcggcaccc gcgcccgcac
     4261 ctactgatgg cagcgccgcg gaatcgccgc ggctgcagac gtccgtcagt ttcaccatca
     4321 ccacggactc caccgcgcag gtcgcggagt tcgtggacaa gctgcggacg ggctcacggc
     4381 tggtgcagat cgtccaggtg ctggtcagca ccggcgacga ggtgccggtc gtagtcaccg
     4441 gcaccgtcac cgccctcatc ttcgtccagc agccctgagg aatgactatg cccgaactca
     4501 ccgcgctcga tcggctgctg gccacggccg tgcaggcccg cgcgtccgac gtgcacctct
     4561 cggccgacca cccgccgctg atgcggctcg atggcgcgct catcggcgtc cccggctact
     4621 ccgaccaact gtcgggagcc tggctggaaa cggcgctgct ggcgattatg gggcctgcac
     4681 ttcgtgacct ctacaaggtg gaatccgagg tcgacctcgc gcactcggtc cccggcatcg
     4741 ggcgattccg ggtgaacgtc tttcaccaga tcgggggagt ggcagccgcg ctgcggctcg
     4801 tcccagagca ggtgaagacc ctggctgaac tcggcattcc gccggtagcg cgcgagctgg
     4861 ccctgcgtcc gcgtgggctg gtgctggtga ccgggcccac cggctcgggc aagtcgacca
     4921 ccctggcggc gatggtcgac gtggtgaacc gtgaggtgcc ggcgcacatc atcacagtcg
     4981 aggacccgat cgagtacatc cacacttcca agcgctcgct gatccaccag cgcgagatcg
     5041 gctcggacac gccttcgttc gcggacgccc tgcggcacat cctgcggcag gatcccgacg
     5101 tcgtgctgat cggcgagttg cgcgacccgg agtcgatctc gatcgccctg accgcagcgg
     5161 agaccggcca gctcgtgctc ggcaccctgc acacccaggg tgcggccaag accatcgacc
     5221 gcatcatcga caccttcccg gcacggcagc agagccaggt gcgctcccag ctcggcgaca
     5281 ccctgcaggg ggtggtcagc cagaccctgc tgccgcgcgc ccagagcggc ggacgcgtgc
     5341 tggccaccga agtgctggtg cacacgccgg ccgtggcgaa cctgattcgt gaaggccagg
     5401 tgcagcagct gtactcggtg atgcagtcca gttcgccgct ggggatgcac accctcgacc
     5461 agagcctcgt ggagctggtc gcgtccggca cggtgtcgcg gcaggtggcg cagggcttcg
     5521 tggtggaccc gcgggcgctg gacaacgtcc gggtacggcc gcaggatctc gacgcggacg
     5581 cctggtcgca gcacgcggcg atgcagcagc gggccagggt gggtgcctga tggcgttgat
     5641 ggaggagttc acctaccagg ccgtctcgcc gaccggaggc ccggtggtca agggcacgct
     5701 ggaggcgtcc ggggcgaacg cggtggcgct gaagctgcgc gcccagggcc tgatgccgct
     5761 gaagatccag gcggtctcga agaccgggtt gaaccgcgac gtgaagctgt tcaaccgcga
     5821 aggccgggtg aaggtgaagc cgctggcgtt gttcgcgaag cagatggccg gcctgatcaa
     5881 cgccggcctg ccgctgatgc gatcgctgtc ggtgctgatc gaacagaccg aggacgcgtc
     5941 actgcggtcg gcgctgaccg cggtgcatgc tgacctggag accggacatt cgttctcggc
     6001 ggcgctggcg aagcagccgc acgccttccc gccgctgatg gtgaacctgg tgaaggtcgg
     6061 cgagactggc ggcttcatgg gcgactcgct ggagttggcc gcgaagagct acggagcgga
     6121 cgccgaactg caggacaagc tgaaagccgc gtccacctac ccgatggtgg tgctggtgat
     6181 cgcggtgctg gccgtgatcg gcatgatcgc cttcatcgtg ccgatcttcg agggcatgtt
     6241 cgcgtccatg ggcggtgagc tgccgttgcc aacccagatc ctggtcacca tgtcgcacaa
     6301 catggtctgg ttgctgccgc tgctgatcgt ggcggtggtg gcgttctggt tctggtggat
     6361 gcgcaaccgc aataccgagg ccgtgcgcaa ggtgatcgac ccgtacaagc tgaagatgcc
     6421 gatcttcggc accctgatga cgaaggtcgc ggtggcccgg ttctcgcgga acctgtcgat
     6481 gatgctggcc gcgggcgtcc cgttgctgca ggcgctgtcg acggtcggga aggcgtccaa
     6541 caactgggcg atcgagcagg ccgtgctcga cgtgcagaag tcggtgcgcg agggcaggtc
     6601 gttcgcggcg ccgctggcga aagccgcggt gttcccgccg atcgtggccc agatggtgtc
     6661 ggtgggtgag gagtcgggca cactgccgga catgctggcc agcatcgccg acatgtacga
     6721 ggcggaggcc aagacggcca ccgaccaatt ggccagcact atcgagccca tcctgatcgt
     6781 gatgatcggc ctgctgatcg gctcgatggt gcttgcgctc tacatgccga tcttcggcat
     6841 ctactcccag ttggccgagc agtaggcggc tggctgggct gaactggggg gcggttcagg
     6901 cgtcggacct gcggagacct tctagtcggg tgtctccgca ggtccgacct ccagaactca
     6961 ggttcgcctc ggctgatcgg gctagtctag attgctcatc cagccgcatg tagatattcg
     7021 tagacatttg gctacccttt ggtctatgaa gaccatcacg gtgggccagt tgcgacagaa
     7081 ccccactgcc atgctcgcgg acgttgaggc cggcgagacc tatcgagtca ctcggcacaa
     7141 ccgggaagtc gcccggctgg tgcccagagg gcgtgaactc gaactgctgc cacgcaaacg
     7201 cgctgccggt tcgcgcctgt cggagttgcc ccgccatgag ttgagcaccg ctgccacgat
     7261 cgacgaactg ctggccgaag agcgtgatcg ttgacgacct tctacctgga tacgtcagtt
     7321 gcccttcgcg cactgctcgg ccactcgccc tcggcggcaa agtggatcga tcaggtgacg
     7381 gctgatgatg agcacctgct ggtctcgtcg cggatgctgc gcaccgagct caccaggatc
     7441 cttcgccgcg aaggcttgcc ggtgaatcgg cgggacgagg tgctggacgt gctgagcctg
     7501 gtgccgatca ccgagggagt gctggctgca gccgaggcga tccaaccgca cgtcaagacc
     7561 ctggacgcga tccaccttgg ctctgtgatt gccgccggcc tcgatgccac gattgtcagc
     7621 catgacgcca ccatgattgc caccgcgacc tcactcggct atccgacctt cgatccggtc
     7681 accgatcggt gagcctgcca ggtttggcag cgacgaaccc aactttggcc ggttatggcg
     7741 gccgccacag atggtgcgga gttgccaacc caactactac cggttatgaa gccgatttca
     7801 ggcctcatgg ccagcagttt ttgggttgag ttcaacgcgg ccgttcgagc agccctgata
     7861 accggtcaaa gctgggttca ttcgtccagc ttgcgccaga ctcgtgtcgt catgaccgac
     7921 ggcgcgaaca tagcttcctt cgcgagccac cgcaccatcc ggtagccgtc gtcacggaag
     7981 acctgctcgc gctccttctc cctcacggcg gcttctgggt cgcggtactt cactttgccg
     8041 tcacactcgc cgatcaggtt gtggtccggc cagtagcagt cggggtacag ggtgccctta
     8101 gcggtgtgga tcggcgggtt gaacagtggc gtgggaatgc cggccagatg gaggtgaccc
     8161 gccgacaacg actccgctgc cgattcccgc tcaggtgtgc acagtgaaat gcccaagcga
     8221 aggcggcccg aacgtatcac cgtagctgcc tccagtaaca agcgccgggc ttcggcgatc
     8281 agcttcgggt tggcgtagtc gcgtcgtctt gggttggcga ccagagattc gcagatgcga
     8341 cgggcggccg catccagcag taccagctgc tcgggcaggg gatggcgccc agcgaggtcg
     8401 actgcggtgc gggcgacaga tgtgacgatg tacccagccg catcacggcc cagatggtgc
     8461 ggcggcaact tgccgatgtg atgattggcc accccgtgtc gcgaccgctg ggtcgtcccg
     8521 gccggaaagg tgatcgcagc cggtgactcg tgccacggcc ggacgccagg aatcggcagg
     8581 ttccatacga ttgctgccga ctcgtggctg agcaccgcct ccgggtacct ggtctgctcg
     8641 gcgtgggcca ggatgaggtg gtggccgacc ggttccgctg gcagagcgga cgccgccagg
     8701 aacacacctg cacggagccg caccagccgg ccagccctga gctgggtctc gatcatggcg
     8761 cgagtgaacc cggc
//
LOCUS       DAOPRQ010000089         8657 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_6791_length_8657_cov_12.053941, whole genome shotgun sequence.
ACCESSION   DAOPRQ010000089 DAOPRQ010000000
VERSION     DAOPRQ010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 8657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8657
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6791_length_8657_cov_12.053941"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            332..1189
                     /locus_tag="PKJ61_10450"
     CDS             332..1189
                     /locus_tag="PKJ61_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HOC14282.1"
                     /translation="MRSTNPVLSKPDTWQVDPAQTQGQYQAQYGQPGQYAQYAQYAQP
                     GQPGGYVQDPYQQAPVVPQAPGRMTIDDVITKSAITMGTLMVVAALTFVFMPAQILFP
                     AAMISAVVAFGTVLLVSFRRALNPAFVLAYAAIEGVFIGAISKVYEDAWNGIVPAALM
                     ATLVAAAATLGAYKFFRIKVTSKFRKMVMIGTIAYAGVLFVNMILSFFGMGFLFSSGN
                     LGLLLLISALGVGLAVFNLILDFDYIEQGIAMGASDTESWRAAFGLTVTMVWLYIEML
                     RLLSYFRSN"
     gene            complement(1253..1966)
                     /locus_tag="PKJ61_10455"
     CDS             complement(1253..1966)
                     /locus_tag="PKJ61_10455"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOC14283.1"
                     /translation="MRIIGVEVAGGTPVFTGPLPHGVDPHRLAWELGYRIVRPLSATG
                     SAPELTLTVQVGRHGRSSELRSAKTRSMDPDLELSDADPPVVRQRLAAYGIVLSRRGL
                     LATQFSDLTAVSGLWGLPGGGIDFGEGPSQALIREITEETGQEAEISHLIDVQTDHWI
                     GRSPSRVVEDFHAVRIIYAARCVEPSDPVVHDWGGTTAAAKWVPIQQWTQTPWSASAK
                     SLLERHLRDLDQAWPDLVS"
     gene            2082..2960
                     /locus_tag="PKJ61_10460"
     CDS             2082..2960
                     /locus_tag="PKJ61_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HOC14284.1"
                     /translation="MQYNENARLDPSQMGSGGGSKRGGTMAIGGGVGILAIILAMVFG
                     INPSSILGGTSGAEQAQTSTNEWAKCQTGADIAADRDCRFVAYTNSIQSYWQQAYPGY
                     EVTQTKLFSGQQATGCGTATSAVGPFYCPVDKVVYLDPSFFDQMLEGQLGAAGGDAAE
                     AYVIAHEYGHHISNLTGVLSQVQSAGLSQAGAKSGQVRLELQADCYAGAWLANSTKDP
                     NSPIKAITQDDLSRAVDAALAVGDDRIQMASTGTVSPESWTHGSSQMRKYWLAQGFNS
                     GDPSKCNTFAADAKLS"
     gene            2924..3982
                     /locus_tag="PKJ61_10465"
     CDS             2924..3982
                     /locus_tag="PKJ61_10465"
                     /inference="COORDINATES: protein motif:HMM:NF014177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - DNA methylation on adenine
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HOC14285.1"
                     /translation="MQHLRGGRQAELIKYLGSKRRLVGELGRIFEHCGATTALDLFTG
                     TTRVAQEFSRLGGFVTCVDTAAYAEVLAQCYVVTDSGQVDADELAEALARLAALPERR
                     GYVTEVFCERARYFHPDNGRRIDAIRAGIDDLYGDHPLRPVLLTALLEAADRVDSTVG
                     LQMAYLKEWAPRALRPLQLVAPKLTPGTGRAIRGDAVALAAELGPFDLAYLDPPYNQH
                     RYYTNYHVWETLVRWDAPEHYGVACKRSDAREAASRSPFNSRRTIAPTLAQVIAEVQA
                     THVVVSFNNEGFVPLDDLIDMCAARGHPVQVFAFDTRRYIGQRLGVFGPDGRRVGEPG
                     PERNVEYVLVSSPAGQVG"
     gene            complement(4325..5257)
                     /locus_tag="PKJ61_10470"
     CDS             complement(4325..5257)
                     /locus_tag="PKJ61_10470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14286.1"
                     /translation="MSTPSRLATAWIAAAAILVSGCAQLETSKSPTAPSATASPSASD
                     FTEPGSATAMVKQLMAKANSTAALMVEITESTVQVSVLDDAHQPTTWAYRGGEIAQVP
                     SDLAYVDQSTFSISRFNINDVGALFRAAAGMAGSEQDQSLTIVDYSGGEVVMSVSTVP
                     ESRTVFFNPDGSLLPVLDFDTPGGIAQGLKEAIGTRSTVASITVESDQGAWVDFPADT
                     ETTTRRTRTQKVPVTTNVRAEKLELPMFNAASVDAAAIWRVVDGLRGTSEIPEQSKWS
                     VVIDDRDALGVPRMHFTIGPKVVVTDLAGNVVST"
     gene            5298..5879
                     /locus_tag="PKJ61_10475"
     CDS             5298..5879
                     /locus_tag="PKJ61_10475"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002528287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HOC14287.1"
                     /translation="MLDLTLDSAALAPLLIGATVRFGEVTIELTEVEAYAGMDDPAAH
                     AFAGPRPHRLDLFAPPGTLYCYLSHGLHICGNITCGDGSGAAILLRAGRVLAGLEVVR
                     ARRSGVADVALARGPGNFGRAIGWTLADSGRRFGEGELELLPATEPRTVASGPRVGVT
                     VAHVRPWRFWADGDPTVSAYRASPRIPPGSQNW"
     gene            complement(5992..6360)
                     /gene="mnhG"
                     /locus_tag="PKJ61_10480"
     CDS             complement(5992..6360)
                     /gene="mnhG"
                     /locus_tag="PKJ61_10480"
                     /inference="COORDINATES: protein motif:HMM:NF009314.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HOC14288.1"
                     /translation="MNVELVLDYLGALSLAFGALFCLAAAIGLVRFPDPLTRMHAATK
                     PSVFGLLLVLLGVTLTLRDPKVFTLLTFAVLMQVMTAPVSGHLVSRTAHRIDQWDAEH
                     AVIDELAADQDDEPPTQAGP"
     gene            complement(6350..6661)
                     /locus_tag="PKJ61_10485"
     CDS             complement(6350..6661)
                     /locus_tag="PKJ61_10485"
                     /inference="COORDINATES: protein motif:HMM:NF015993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter complex subunit
                     F"
                     /protein_id="HOC14289.1"
                     /translation="MIVIIGIAALLLFASAVLTLWRMVQGPTGLDRIIASDVMVAIAI
                     AAAALYSVLARNATGLPILLGLSLVGFTGAVGVARLITSSAGVRKLVSRRKAMKDDDE
                     R"
     gene            complement(6658..7194)
                     /locus_tag="PKJ61_10490"
     CDS             complement(6658..7194)
                     /locus_tag="PKJ61_10490"
                     /inference="COORDINATES: protein motif:HMM:NF006521.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HOC14290.1"
                     /translation="MNASNRLRFRFQWRAIVLLALVWVLLWGDASLLNILYGALLGWL
                     VTVVFWLAPIRYYGRVHVGRALLLLFTQLRDLAVASVHLAMVAIRPRLELRPGVVKVT
                     LRSNSDLYQVMVAQLISIVPGTLVVEVSRHPRLLFLHVFDLPDAAAVKRERKHALALE
                     RRVVEAFGSKSDIEAVRQ"
     gene            complement(7191..>8657)
                     /locus_tag="PKJ61_10495"
     CDS             complement(7191..>8657)
                     /locus_tag="PKJ61_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit D"
                     /protein_id="HOC14291.1"
                     /translation="LAGRHPAVQRAISVTAVAAVCAVAGVLVWATDTAPLVLWVGDWP
                     QPLGIVLVADRLGALMLLVAALVSLAVLVYSTGQDEDEMRRETPVSVFHPTFLLLVAG
                     VSNAFLAGDLFNLFVSFELLLFASYVLLTMGGTEDRIRAGSVYVVVNLVSSSIFLVAI
                     ASTYAATGTVNLAQLAIRLGELAPEVRGVLQVMLLAVFAIKAAVFPLSAWLPHSYPTA
                     PAPVTAVFAGLLTKVGVYAIIRTQTLLFQAEPLTVVLGFAAVATMLIGILGAIAQVEI
                     KRMLSFTLVSHIGYMMFGIALANPRALAGSIFYAAHHITIQGTLFLVAGLMERVGGST
                     SLDRLGGLARISPLLGALFFVPAMNLAGIPPLSGFVAKLALLQAGVADGTPMAWALVV
                     TGVVTSLLTLYAMAKVWNRAFWSTAPETVVESARSYDSPARGRLLPYLMVGSTVGLIA
                     LTLTFTVAAGPMYDYVERSASALFDRSYITAVLPEGVR"
BASE COUNT         1472 a         2948 c         2883 g         1354 t
ORIGIN      
        1 gaggcaagcg gcttaaaccc gctcaagtat gggttcgaat cccattgggg gcacggttga
       61 aagtcacacc gagacgcatg atttggtagc ccgccacggc cagaccgtcg tggcttacgc
      121 caccaaagct gagactcttg gcctacccca ccccagctgg aacactaatc cgagcctgtc
      181 gagacccgac cgaaattcag ggttgcggat cagggttggc ccggggcgga acacagcagc
      241 ctctcaggag cgttatctac catggaaccc gacgacggtc cccgcgcctg aagatgggag
      301 catccgtcgc ccacccaagg aggaacaccc aatgcgcagc accaacccgg tcctgtccaa
      361 gccggacacc tggcaggtcg acccggcaca gacccagggc cagtaccagg ctcagtacgg
      421 ccagccgggc cagtacgccc aatacgccca atacgctcag cccgggcagc cgggtggcta
      481 cgtccaggat ccctaccagc aggcaccagt cgtgccgcag gctccgggac ggatgaccat
      541 cgacgacgta atcaccaaga gcgccatcac gatgggcacg ctcatggtcg tcgcggcact
      601 caccttcgtg ttcatgccgg cccagatcct gttcccggca gctatgatct cggcagtggt
      661 cgcgttcggc accgtgctgc tggtcagctt ccggcgcgcg ctgaacccgg ccttcgttct
      721 cgcctacgcg gccatcgagg gcgtgttcat cggtgcgatc agcaaggtgt atgaggacgc
      781 ctggaatggc atcgtgcccg cggcactgat ggccaccctg gtcgctgctg ccgccacgct
      841 gggcgcgtac aagttcttcc ggatcaaggt caccagcaag ttccgcaaga tggtgatgat
      901 cgggacgatc gcctacgccg gcgtgctgtt cgtgaacatg atcctgtcct tcttcggcat
      961 gggcttcctg ttctccagcg gcaacctcgg cctgctgttg ctgatctcgg cgctcggcgt
     1021 cggtctggcc gtgttcaacc tgatcctcga cttcgactac atcgagcagg ggatcgccat
     1081 gggcgccagc gacaccgagt cctggcgtgc ggcgttcggt ctcaccgtca cgatggtgtg
     1141 gctgtacatc gagatgttgc ggctgctgtc gtacttccgc tccaactagc tcgaacctga
     1201 acgagggccc cggtgtgccg ccggggccct cgtcgcgttt cggttcggtc gatcagctga
     1261 ccaggtcggg ccaggcttga tccaggtccc gcaggtgccg ctccaacagc gacttcgcgc
     1321 tggccgacca cggagtttgc gtccactgct ggatcggcac ccacttcgcg gccgcggtcg
     1381 tcccgcccca gtcgtgtacc accgggtcgg acggttcgac gcagcgcgcg gcgtagatga
     1441 tccgcacggc atggaagtcc tcgaccaccc gcgacggcga gcggccgatc caatggtcgg
     1501 tctgcacatc gatcaggtgg gagatctccg cctcctgacc ggtctcctcg gtgatctcgc
     1561 ggatcagcgc ctggcttggt ccttccccga agtcgatgcc cccgcccggc agcccccaca
     1621 gcccgctcac cgcggtgagg tcggagaact gcgtcgccag cagcccgcgc cgcgacagca
     1681 cgatgccgta cgcggccagc cgctgccgca cgaccggcgg gtcggcgtcc gacagttcca
     1741 gatcgggatc catcgagcgg gtcttcgccg aacgcaactc actgctgcgg ccgtgccggc
     1801 caacctgaac ggtgagggtc agctcgggcg cgctgccggt tgccgagagc ggacggacga
     1861 tgcggtagcc cagttcccag gcgaggcggt gcgggtcgac cccgtgcggc aagggtccgg
     1921 tgaacaccgg ggtacccccg gccacctcga caccgatgat ccgcatctgt ccatcatggc
     1981 accaagtccg cggcaaccaa gccggccacc cgcccggcaa cccagccgcg ctaacccgcg
     2041 gtggatatgc tcatcctggc gtctggagtc aggagtcagt tatgcagtac aacgagaacg
     2101 cacggctcga cccgtcccag atgggctccg gcggcggcag caagcgcggc ggcacgatgg
     2161 ccatcggtgg tggtgtcggc atcctggcca tcatcctggc gatggttttc ggcatcaatc
     2221 cgagttcgat cctcggtggc accagcggcg ctgagcaggc ccagacctcg acgaacgagt
     2281 gggccaagtg ccagaccggc gccgacatcg ccgccgatcg tgactgccgc ttcgtcgcct
     2341 acaccaactc gatccagtcc tactggcagc aggcgtaccc cggttacgag gtcacccaga
     2401 ccaagttgtt cagcggccag caggccaccg gttgtggcac cgcgacgtcg gccgtcgggc
     2461 cgttctactg cccggtggac aaggtcgtct acctcgaccc gagcttcttc gaccagatgt
     2521 tggagggcca gctcggcgct gccggcggcg atgccgctga ggcctacgtg atcgcccacg
     2581 agtacgggca ccacatctcc aacctcacgg gtgtgctctc gcaggtgcag tcggctgggc
     2641 tctcccaggc cggcgccaag tcgggccagg tacgccttga acttcaggcc gactgctacg
     2701 caggggcgtg gctggccaac tccaccaagg acccgaactc gccgatcaaa gccatcaccc
     2761 aggatgacct gagccgggcc gtggacgcag ccctcgccgt cggcgacgac cggatccaga
     2821 tggcctccac cggtacggtt tcgccggagt cgtggacgca cggctcgtcg cagatgcgca
     2881 agtactggct cgcccagggc ttcaacagcg gcgacccgag caagtgcaac accttcgcgg
     2941 cggacgccaa gctgagttga tcaaatacct cggctcgaag cgccgcctgg tcggcgagtt
     3001 gggccggatc ttcgagcatt gtggcgccac gaccgcgctc gacctgttca ccggcaccac
     3061 ccgggtggcc caggagttct cccggctcgg tgggttcgtg acctgcgtcg acaccgccgc
     3121 ctacgccgaa gtgctcgcgc agtgctacgt ggtcactgac tccggccagg tcgatgccga
     3181 cgagttggcc gaggcgctgg cgcggctggc cgcgctgccg gagcggcgtg gctacgtcac
     3241 cgaggtgttc tgcgagcggg cacgctactt ccatcccgac aacggtcgcc ggatcgacgc
     3301 gatccgggcc ggtattgacg atctctacgg cgaccaccca ctgcgtccgg tgctgttgac
     3361 cgcgctgctg gaggccgccg atcgggtcga ttccaccgtc ggactgcaga tggcctacct
     3421 caaggagtgg gctccccgcg ctctgcgtcc gctgcagttg gtcgccccga agctcacgcc
     3481 cggcactggt cgggcgatcc gcggggacgc ggtcgcgctg gccgctgaac ttgggccgtt
     3541 cgacctcgcc tatctcgacc cgccctacaa ccagcaccgc tattacacca actaccacgt
     3601 ctgggagacg ctggtccgct gggacgcccc cgagcactac ggggtcgcct gtaagcgctc
     3661 ggacgcgcgc gaagccgcca gccggagccc gttcaactcg cggcgcacca tcgcaccgac
     3721 gctggcccag gtgatcgctg aggtgcaagc cacccacgtg gtggtttcgt tcaacaacga
     3781 gggttttgtg ccgctcgacg acctgatcga catgtgcgca gcgcgcgggc acccggtgca
     3841 ggtcttcgcc ttcgacaccc gccgctacat cgggcagcgg cttggcgtct tcggtccgga
     3901 cggacgccgg gtcggcgagc ccggaccgga gcgcaacgtc gagtacgtcc tggtcagcag
     3961 tccggccggc caggtcggct gagccggtcg aagccgatcc tccgcaagcg ggaaacggct
     4021 tcgcctaccg cgattccgac gcgcgagcac tccggacggg gcggcgggct cacggccaag
     4081 ttggcccgcc aaggcacgaa ttgcgtcatg cgtcggccac ggccgcgttt ccccagccga
     4141 cgcttggcgg gtgtgaccgg cctggattgt cgcgatctcg cgatgatcgc gccgggctac
     4201 agctggctcc cggcgtcggc ttcgtagctc agcttcgaga ttgcgacagc ccgccgccgc
     4261 ctccgtccgc gagaggtggc ctgagcctcg gcaagttcag gggttcgtcc agactcagct
     4321 tggttcaggt gctgaccacg ttccccgcaa gatcggtgac cacaactttc gggccgatcg
     4381 tgaagtgcat ccgcgggacg ccaagtgcgt cccggtcatc gatgacgacg ctccacttcg
     4441 actgttccgg gatctcggac gtgccacgca gcccgtcaac cacccgccaa atggcggccg
     4501 cgtcgaccga ggcggcgttg aacatgggga gttcgagctt ctccgcgcgg acgttggtgg
     4561 tcaccggcac cttctgagtg cgggtccgcc tggtggtcgt ttcagtgtcc gcggggaaat
     4621 ccacccaggc accctggtca gattccacag tgatcgatgc gaccgtggac cgcgtcccga
     4681 tcgcctcctt caggccctgt gcgattccgc cgggggtgtc gaagtcgagc actggcaaca
     4741 ggcttccgtc cgggttgaag aacaccgttc gggactccgg cacggtcgag accgacatca
     4801 ccacctcacc gccggagtag tcgacgatgg tcaggctctg gtcctgctcg gaaccggcca
     4861 tgccggctgc ggctcggaaa agggcgccga catcgttgat attgaacctg gagatgctga
     4921 acgtcgactg gtccacgtaa gccaggtcgc tgggcacctg cgcgatctcg ccgccgcggt
     4981 acgcccaggt cgtgggctga tgggcgtcgt ccagcacgct cacctggacg gtcgattcgg
     5041 tgatctccac catcagcgct gcggtgctgt tcgccttcgc catcagttgc ttgaccatcg
     5101 cggtcgcaga cccgggctcg gtgaagtcgg aggcggacgg gctcgcggtc gccgagggtg
     5161 cggttggtga cttggaggtt tcgagctgtg cgcaccccga gaccaagatc gcggccgcag
     5221 cgatccaggc ggtcgcgagt cgagacgggg tgctcacctg tcgagcttac cgtcagcccg
     5281 attggcagac tgggggcgtg ctcgacctca ccctggactc cgcggccctg gccccgctgc
     5341 tgatcggggc cacggtgcgt ttcggtgagg tgacgatcga gctgaccgag gtggaagcgt
     5401 acgcggggat ggatgatccg gccgcgcacg cgttcgccgg gccgcggcca cacaggttgg
     5461 acctgttcgc gccgcccgga acgctctact gctacctctc ccacggcctg cacatctgcg
     5521 gcaacatcac ctgcggcgac ggctcgggtg cggcgatcct gctgcgtgcc ggacgcgtgc
     5581 tggccgggct ggaggtagtg cgcgcccgcc gttcgggggt tgcggacgtg gcgctggccc
     5641 gaggcccggg caatttcggg cgtgcgatcg gctggacgct ggctgattcg ggacgccgct
     5701 tcggcgaggg cgagctggaa cttctgcctg cgaccgaacc acggacggtt gcatccggcc
     5761 ctcgggtcgg ggtgaccgtg gcgcacgtcc gcccgtggcg cttttgggcc gacggcgatc
     5821 cgaccgtctc ggcctaccgg gcgagccccc ggatcccgcc gggcagccag aactggtgac
     5881 cggcggtctg agcaccgggg acggttccag atacgtcatc ccggcgtagg ccgggatgac
     5941 cttcggtgtc caactagggg ccggctgaac tcatccggcc agttgggtag ctcagggacc
     6001 ggcttgcgtc ggcggctcgt cgtcctggtc ggcggccaac tcgtcgatga cggcgtgctc
     6061 ggcgtcccac tggtcgatgc gatgggcggt ccgggagacc aggtggccgg acaccggggc
     6121 ggtcatcacc tgcatcagca cggcgaaggt gagcagggtg aacactttcg ggtcccgcag
     6181 ggtgagggtc acgccgagca gcaccagcag cagtccgaag acggagggct tggttgccgc
     6241 gtgcatccgg gtcagcgggt cggggaaccg caccaggccg atcgcggcgg ccaggcagaa
     6301 cagggcgccg aaggccaggc ttagcgcccc gagataatcc agcaccagtt caacgttcat
     6361 cgtcgtcctt catggccttc cggcggctga ccagcttgcg gacgcccgcc gacgaggtga
     6421 tcagccgggc cactccgacc gccccggtga atccgaccag cgacagcccc aacaggatcg
     6481 gcaggccggt cgcgttgcgg gcgagcaccg agtagagagc cgccgcggcg atggcgatcg
     6541 cgaccatcac gtccgaggcg atgatgcggt cgagcccggt cggaccctgc accatgcgcc
     6601 agagtgtgag caccgcggac gcgaacagca gcagcgccgc gattccgatg atgacgatca
     6661 ttgccgcacc gcctcaatgt ccgacttgct gccgaacgcc tccaccaccc ggcgctcgag
     6721 cgccagggcg tgcttgcgtt cgcgctttac cgcggccgcg tccggcaggt cgaagacgtg
     6781 caggaacaac aggcgcgggt gccggctcac ctccacgacc agggtgccgg ggacgatcga
     6841 gatgagttgc gcaaccatca cctggtagag gtcgctgtta ctgcgtagcg tgaccttgac
     6901 gacgcccgga cgcaactcca gtcgcggccg gatcgccacc atcgccagat gaacggacgc
     6961 caccgccaga tcccgcagct gtgtgaacag caacagcagc gcccggccga catgcacccg
     7021 tccgtagtac cggatcgggg ccagccagaa caccacggtc accagccagc cgagcagcgc
     7081 gccgtagagg atgttcagca gggacgcatc cccccagagc agcacccaga ccagggccaa
     7141 caggacgatt gcacgccact ggaagcggaa ccgaagccga ttggaggcgt tcatcgcacc
     7201 ccctccggga gcacggcggt gatgtagctg cggtcgaaca acgctgaagc ggaccgttcc
     7261 acgtagtcat acatcggccc ggcggcaacc gtgaaggtga gcgtgagcgc gatcagcccc
     7321 acggtcgagc cgaccatcaa atacggcagc agccggccgc gagcgggtga gtcgtagctg
     7381 cgcgccgact ccaccactgt ttccggtgcg gtgctccaga acgcccggtt ccagaccttg
     7441 gccatggcgt agagcgtcag cagcgaggtc accacaccgg tgacgaccaa tgcccaggcc
     7501 atcggcgtcc cgtccgcgac acctgcctgt agcaacgcga gcttggccac gaagccggac
     7561 agcggcggga tgccggccag gttcatcgca ggtacgaaga acagcgcccc gagcagcggc
     7621 gagatccttg ccaggccacc cagccgatcc agcgatgtgg atccgccgac ccgttccatc
     7681 agcccggcca ccaggaagag ggtgccttga atggtgatgt ggtgggcggc gtagaagatc
     7741 gaaccggcca gcgcgcgcgg gtttgccagc gcgatgccga acatcatgta gccgatgtgg
     7801 ctgaccaggg tgaacgacag cattcgcttg atctcgacct gtgcgatcgc gccgaggatg
     7861 ccgatcagca tcgtggccac cgcggcgaag cccagcacca cggtgagcgg ctcggcttgg
     7921 aagagcaggg tctgcgtccg gataatcgcg tagacaccca ccttggtgag caggccggcg
     7981 aacacggcgg tcaccggagc cggagcggtc gggtaggagt gcggcagcca ggcagagagg
     8041 gggaagaccg cggctttgat cgcgaatacc gccagcagca tgacctgcag cactccgcgc
     8101 acctcggggg ccagttcgcc gaggcggatg gccagctgcg ccaggttcac cgtgccggtc
     8161 gccgcgtagg tgctggcgat cgccaccagg aagatcgacg acgagaccag gttcaccacc
     8221 acgtacacgc ttccggcgcg gatccggtcc tcggtgccgc ccatggtcag cagcacatag
     8281 gacgcgaaca gcagcagttc gaagctgacg aacaggttga acaggtcacc ggcgaggaac
     8341 gcgttcgaaa cgccggccac cagcagcagg aaggtggggt ggaacaccga gaccggggtc
     8401 tcgcggcgca tctcatcctc gtcctggccg gtggaataga ccagcaccgc cagcgagacc
     8461 agcgcggcta ccaggagcat caaggcgccc agccggtcgg cgaccagcac gatgccgagc
     8521 ggctgcggcc agtcgccgac ccagagcacc agcggcgcgg tgtcggtggc ccagaccagg
     8581 acgccggcga ccgcgcacac cgcggccacc gcggtgactg agatcgcgcg ctggacggca
     8641 ggatggcggc cggccag
//
LOCUS       DAOPRQ010000090         8391 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_7058_length_8391_cov_12.207893, whole genome shotgun sequence.
ACCESSION   DAOPRQ010000090 DAOPRQ010000000
VERSION     DAOPRQ010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 8391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8391
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7058_length_8391_cov_12.207893"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1082)
                     /locus_tag="PKJ61_10500"
     CDS             complement(<1..1082)
                     /locus_tag="PKJ61_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008362273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HOC14292.1"
                     /translation="MSKKIWFLTGSQGLYGDDVLAQVASQSRQISDALAAAAQIPVEV
                     VAKPTLTDATAIRRIMLDANADDDCVGVIAWMHTFSPAKMWITGMDALTKPFLHLHTQ
                     VNVELPWATIDMDFMNLNQAAHGDREFAYIATRLGVPRKTVAGHVSDPRVIDRVGSWA
                     RAALARAELATLKLVRFGDNMRNVAVTEGDKVEAERRLGVSVNTYGVNDLVAVVDAVT
                     EAEIDPLIAEYADLYEVAAELLPGGDRHDSLRYGARIEIALRKFVIDGGYGAFTTNFE
                     DLGGLRQLPGLGVQRLMGEGIGFAGEGDWKTAVLGRTLMTMADGLPGGTSFMEDYTYH
                     LVPGQELSLGAHMLEVSPLIAGETPR"
     gene            complement(1079..1780)
                     /locus_tag="PKJ61_10505"
     CDS             complement(1079..1780)
                     /locus_tag="PKJ61_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015070798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HOC14293.1"
                     /translation="MDVTRTAIGGELRDVVEQLKIEVAALHAELPRNGLVVWTAGNVS
                     ARVPGAELLVIKPSGVSYDELTPEAMVVCDFEANLVEGSRSPSSDTAAHAYVYKHMAD
                     VGGVVHTHSDYATAWAARREPIPCVLTMIADEFGGEIPVGPFALIGDDSIGRGIVETL
                     RESRSRAVLMANHGPFTIGRDARDAVKAAVMVEDVARTVHIARQLGEPVPMAQADIDR
                     LFDRYQNVYGQGNDK"
     gene            complement(1891..2646)
                     /gene="recO"
                     /locus_tag="PKJ61_10510"
     CDS             complement(1891..2646)
                     /gene="recO"
                     /locus_tag="PKJ61_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015070170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOC14294.1"
                     /translation="MPTYRDEAVVLRTHKLGEADRIITLLSRQHGKVRAVARGVRRTS
                     SKFGARLEPFSHVDLQFATGRTLDVITQAVTLHAYSEPLAHDYERYTTGQVLVETADR
                     LVTEVGEPAVQQYQLLVGALHALTGGGADAPRPAAMVMDAYLLRATALAGYAPSLSDC
                     ARCGASGPHEAFSPQLGGMVCRRCRPPGSAHPDPATVAYLVALLTGDWAATEQVSATI
                     VRQGNGLVAAFVGWHLERGLRSLSHVDRSEPTR"
     gene            2763..4358
                     /locus_tag="PKJ61_10515"
     CDS             2763..4358
                     /locus_tag="PKJ61_10515"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HOC14295.1"
                     /translation="MSQSVVEVIAGGRTALGIEFGSTRIKAVLVGADNAPLATGAYDW
                     ENQFVDRVWTYSLADVRTGLQAAYASLSADVKARYGVDLTTIGALGVSAMMHGYLAFD
                     ASGELLVPFRTWRNTITGAASGKLSALFGHNIPQRWSIAHLYQAILNGEEHVGRVAHV
                     TTLAGYVHAQLTGEQVLGVGDASGMFPIDINTGDYNAAMLTQFDDLVADSGFDWRLGD
                     ILPKVLPAGEVAGRLTEAGAQLLDPTGKLAAGIPLCPPEGDAGTGMVATNSVARRTGN
                     VSAGTSIFAMIVLEHELSQVHDELDLVTTPAGDLVAMVHCNNGASELNAWAGLFGEFA
                     KRLGSDADQNAVFETLFAASLEGAADCGGLLAYNYLSGEHITGLVEGRPLFTRTPDSQ
                     FNLANFMRTHLFTALSSLRVGMEVLLKAEQVQLDSMFAHGGLFKTRGVAQRYLAAAIN
                     APVSVGDIAGEGGAWGIALLAAFARDRVEGQRLDEYLNSRAFANAALETIEPDATDVA
                     GFDAYVSRFVRGLSIEKTAVEHS"
     gene            4399..5022
                     /locus_tag="PKJ61_10520"
     CDS             4399..5022
                     /locus_tag="PKJ61_10520"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HOC14296.1"
                     /translation="MGILRITVDDTATVAASSAKLHLVVKGAAAVLGNAAVKRAAEVR
                     ELVAALAKAGIGEDKVEVAGVQLSSSTNLLGRNQRAEISLTVSLEPDQLPSALGILTD
                     SPNLTLESLEWVFDTFEASIPLTAGAMVKARRKADAVAAAAGLRITGITNASDSWSMV
                     APREALAYGAPMAARSMKSSADLDLGLDYSSSTQLSVHVSVDFEMAE"
     gene            5074..6012
                     /locus_tag="PKJ61_10525"
     CDS             5074..6012
                     /locus_tag="PKJ61_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015069845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOC14297.1"
                     /translation="MKRIADLRVSPVCLGGNVFGWTADSAASEQILDAYADAGQNFID
                     TADAYSAWVPGHSGGESESLIGGWLRKRRLRDRMVIATKVGKHPSRRGLRPANLRVCL
                     DGSRRRLGVETIDLYYAHSDDAHVPVAEWVGAFDAMIADGAIRHYGLSNFSAERTTEV
                     LEVAKAEGLAAPVVLQPQYHLVHRSEVEAGGLAELAAANGLAIVPYYGLAAGFLTGKY
                     RADEPMHGARAARVKHWANAEGFAVVDAVVDIANDLDVEPATVALAWLRQQPGVTAPI
                     ASVSTVEQLPAVLAAGELKLTRGQLNRLNKVSAPFA"
     gene            complement(6017..6448)
                     /locus_tag="PKJ61_10530"
     CDS             complement(6017..6448)
                     /locus_tag="PKJ61_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015069990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOC14298.1"
                     /translation="MARSYDPFRDLDRWFSEAPRTPASTAMPMDLYRDGDVFVARIDL
                     PGVDPSSIDIDVDDRTLTVRAERRAETGADAKWLVRERPAGTFARQLTLGYGVALDRI
                     SAEYSDGVLNLKIPVAEEAKPRKISVTHSGGPTQIEGSVES"
     gene            6606..7394
                     /locus_tag="PKJ61_10535"
     CDS             6606..7394
                     /locus_tag="PKJ61_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002513780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HOC14299.1"
                     /translation="MSVPRQFVEPSPHPSGARPPQLDPKLLPQHVAIVMDGNGRWAKA
                     RNLPRTEGHTRGEAALFDVIEGAIELGIKYLSAYAFSTENWRRSPDEVRWLMGFNRDV
                     IHRRRDQFVEMGVRVRWAGRTPKLWRSVINELQEAERMSAGNDRITLQFCVNYGGRAE
                     IVDAARAIAAAAQRGELDPSRLTEDRFRRFLDEPEIPDVDLFVRSSGEQRTSNFLLWQ
                     SAYAELVFLDRLWPDFDRRDLWRAVEMYVQRDRRYGGAVPNVLS"
     gene            complement(7519..>8391)
                     /locus_tag="PKJ61_10540"
     CDS             complement(7519..>8391)
                     /locus_tag="PKJ61_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HOC14300.1"
                     /translation="YRDSKEKAYSTDANIWGATHEAKELENLNVGIEIVEPIMGVRFW
                     DPSVEIATEDVTITFEQGRPTQINGRTFATAVDLVREANTIGGRHGLGMSDQIENRII
                     EAKSRGIYEAPGMALLHICYERLVTAIHNEDTFASYQLEGRKLGRLMYEGRWLDPQSL
                     MIRESLQRWVGSAVTGSVTLRLRRGDDYSILDTAGPSLSYHPERLSMERVGDAAFGPL
                     DRIGQLTMRNLDIADSRARLEQYAQLGLVGGEVSALVGQLEAGTAPAIAAGEGTDSEI
                     EAAQTRSGMEAGTD"
BASE COUNT         1340 a         2829 c         2843 g         1379 t
ORIGIN      
        1 atgcgggggg tctccccggc gatcagcggc gagacctcga gcatgtgggc gccgaggctc
       61 agttcctggc ctggcacgag gtggtaggtg tagtcctcca tgaaggaggt gccgcccggc
      121 agcccgtccg ccatggtcat cagggtgcgg ccgagcacag cggtcttcca gtcgccctcg
      181 ccggcgaaac cgatgccctc acccatcagc cgctgcacgc cgaggccggg gagctggcgc
      241 agcccgccga gatcctcgaa gttggtggtg aaggcgccgt agccgccgtc gatcacgaac
      301 ttgcgcaggg caatctcgat ccgggcgccg tagcgcagcg aatcgtggcg atcgccgccc
      361 ggcaggagct cggcagccac ctcgtagagg tcggcgtact cggcgatcag cgggtcgatc
      421 tcggcttcgg tgaccgcgtc caccactgcc accaggtcgt tgacgccgta ggtgttgacc
      481 gagacgccga ggcgacgctc ggcttcgacc ttgtcacctt cggtgaccgc gacattgcgc
      541 atgttgtcgc cgaagcgcac cagcttgagg gtggccagtt ccgcgcgagc cagcgcagcg
      601 cgcgcccagc tgccgacccg atcgatcacc cgcggatcgc tcacgtggcc ggccacggtc
      661 ttgcgcggga cgcccagccg ggtggcgatg taggcgaact cacggtcgcc gtgggcggcc
      721 tggttgaggt tcatgaagtc catgtcgatg gtcgcccagg gcagttccac gttcacctgg
      781 gtgtgcaggt gcaggaacgg cttggtcagc gcgtccatgc cggtgatcca catcttcgcc
      841 ggcgagaagg tgtgcatcca ggcgatcacg ccgacgcagt cgtcatcggc attggcgtcg
      901 agcatgatcc gccggatcgc ggtcgcgtcg gtcaacgtcg gcttggcgac gacttcgacc
      961 gggatctgcg cagccgcggc cagcgcgtcc gagatctggc gcgactggct ggcgacctga
     1021 gccagcacgt catcgccgta caggccctgg cttccggtga ggaaccagat cttcttgctc
     1081 atttgtcgtt tccttgtcca tagacgttct ggtagcgatc gaaaagccgg tcgatgtcgg
     1141 cctgcgccat cggcaccggc tcgcccaact ggcgggcgat gtgcacggtg cgagcaacgt
     1201 cctcgaccat caccgcggcc ttcaccgcgt cccgggcgtc gcgcccgatg gtaaacgggc
     1261 cgtgattggc catcagcacc gctcgggagc ggctttcccg cagggtttcg acgatgccgc
     1321 gcccgatcga gtcatcgccg atcagggcga aggggccgac cgggatctcg ccgccgaact
     1381 cgtcggcgat catcgtgagt acgcagggaa tcggctcgcg acgcgccgcc caagcggtcg
     1441 cgtagtcgga atgggtgtgc accacaccgc cgacgtccgc catgtgcttg tacacatagg
     1501 cgtgcgcagc ggtgtcggac gaaggactgc gactgccctc gaccaggttc gcctcgaagt
     1561 cgcagaccac catcgcttcc ggggtgagtt cgtcgtagga gacccccgac ggcttgatca
     1621 ccaacagctc ggctcccgga accctggccg acacattgcc ggcggtccag acgaccagtc
     1681 cgttgcgggg aagctcggca tgcagcgcgg ccacttcgat cttgagctgc tcaacgacgt
     1741 cgcggagttc gcccccgatc gcggtccgag tcacatccat gtgtcgtctt ccaatctcgc
     1801 ttcagcgcgc caacaatcgg aatgttaccg atatcacagc tcgggtgatg caagtggcga
     1861 taacggtttg gtatcgaatc aagaccgggc tcagcgagtc ggctcggacc ggtcgacgtg
     1921 gctgagcgac cgcagcccgc gctccagatg ccagccgacg aaggctgcca ccagcccgtt
     1981 tccctgccgg acgatggtcg cggaaacctg ctcggtcgcc gcccagtcac cggtcagcag
     2041 cgccaccaga taggcgacgg tggccggatc ggggtgcgcc gaaccgggcg gacggcagcg
     2101 ccggcagacc atgccgccca gctgcggaga gaacgcctca tgcggcccgg acgcaccaca
     2161 gcgggcgcag tcggacagcg atggggcgta gccggccagg gccgtcgcgc gcagcaggta
     2221 ggcgtccatg accatggccg cgggccgcgg tgcgtccgct ccgccgcccg tgagcgcgtg
     2281 cagggccccg accagcaact ggtactgctg gacggccggc tcccccacct cagtgacgag
     2341 tcggtccgcg gtctccacca gcacctggcc ggtggtgtag cgctcgtagt cgtgggcgag
     2401 cggctcggag taggcgtgca gggtcaccgc ctgggtgatc acgtccagcg tgcgtccggt
     2461 ggcgaactgc aggtcgacgt ggctgaacgg ctccaaccgc gcaccaaact tggacgaggt
     2521 tcgccgcacg cccctggcca ccgcgcgcac cttgccgtgc tgccgggaga gcagcgtgat
     2581 gatccggtcg gcctcaccca gcttgtgggt gcgcaggacg accgcctcgt cacgataggt
     2641 gggcaccagg ccattgtccc tgcccacctg atcggctcgg gtcaggagag ctccggcgcg
     2701 ccgttgttag cgcacacaca cgcctgccat gatggttcca gcactcagtc aaggaggatc
     2761 aggtgagcca atccgtagtc gaggtgatcg caggaggccg gaccgccctg ggcatcgagt
     2821 tcggttccac gcgcatcaag gcggtgctgg tcggtgccga caacgccccg ctcgccaccg
     2881 gcgcctacga ctgggagaac cagttcgtcg accgggtgtg gacctactcc ctggccgacg
     2941 ttcgcaccgg cctgcaggcc gcctacgcct cgctgagcgc tgatgtgaag gcccgctacg
     3001 gcgtggactt gaccacgatc ggcgcgctcg gcgtgtcggc gatgatgcac ggctacctcg
     3061 ccttcgatgc gtccggcgag ctgttggtgc cgttccgcac ctggcggaac acgatcaccg
     3121 gcgcagcgtc ggggaagttg agcgcgctct tcggtcataa catcccgcag cgctggagca
     3181 tcgcgcacct ctaccaggcg atcctgaacg gcgaggagca tgtcgggcgc gtcgcgcacg
     3241 tcaccacgct ggccggctac gtgcacgccc agctgaccgg cgagcaggtg ctgggtgtcg
     3301 gcgacgccag cggcatgttc ccgatcgaca tcaacacggg tgactacaac gccgcgatgc
     3361 tcacccagtt cgacgacctg gtcgccgatt ccggcttcga ctggcgcctc ggcgacatcc
     3421 tgccgaaggt gctcccggcc ggcgaggtgg ccggacgcct gaccgaagcc ggcgcgcagt
     3481 tgctcgatcc gaccggaaag ctggccgccg gcatcccgct ttgcccgccc gagggcgatg
     3541 ccggcaccgg catggtggcc accaactcgg tggcccgccg caccggcaac gtgagcgcgg
     3601 gcacctcgat cttcgcgatg atcgtgctcg agcacgagct cagccaggtg cacgacgagc
     3661 tcgacctggt caccaccccg gccggcgacc tggtggcgat ggtgcactgc aacaacggcg
     3721 ccagcgagct gaacgcctgg gccggcctgt tcggtgagtt cgccaagcgc ttgggttcgg
     3781 acgccgacca gaacgcggtc ttcgagaccc tgttcgccgc ttcgcttgag ggcgcggccg
     3841 actgcggtgg gctgctggcc tacaactacc tctccggcga acacatcacc ggtctcgttg
     3901 agggtcgacc gctgttcacc cgcacccccg acagccagtt caacctggcc aacttcatgc
     3961 gaacccacct cttcacggcc ctttccagcc tgcgggtggg catggaggtg ctgctgaagg
     4021 cggagcaggt gcagctcgac tcgatgttcg cccacggcgg cctgttcaag accaggggcg
     4081 tcgcccagcg ttacctcgcc gcagcgatca atgccccggt ttcggtcggc gacatcgccg
     4141 gcgagggtgg cgcctggggt atcgccctgc tggccgcgtt cgcacgtgat cgcgtcgagg
     4201 gccagcggct cgacgaatac ttgaacagcc gtgccttcgc caatgcggcg ctggagacca
     4261 tcgagcccga cgccaccgac gtggccgggt tcgacgccta cgtcagccgg ttcgtccgcg
     4321 ggttgtcgat cgagaagact gcagtcgaac acagctgaac cgctaccgga gccgcgctgg
     4381 ccgccctacg ctgccggcat gggaatcctg cggatcacag tcgatgacac cgctacggtc
     4441 gccgcaagtt cggccaagct gcacctggtg gtgaaaggtg cggcagccgt cctcggcaat
     4501 gccgcggtga agcgcgcggc cgaggtgcgc gagctggtgg cagcactggc caaggcgggc
     4561 atcggcgagg acaaggtaga ggtggccggc gtccagctga gctcgtccac caacctgctg
     4621 ggacgcaacc agcgggccga gatctccctg acagtttcgc tcgaacccga ccaactaccc
     4681 agcgcgctcg gcatcctgac cgattcgccg aacctgaccc tggagagcct cgagtgggtc
     4741 ttcgacacgt tcgaggcgag catcccgctc accgcagggg ccatggtcaa ggccaggcgc
     4801 aaggcggacg cggtcgcggc ggcggcgggc ctgcgaatca ccggcatcac gaacgccagc
     4861 gactcctgga gcatggtcgc gccccgcgag gcgctggcct acggcgcgcc gatggccgca
     4921 cgctcgatga agagctccgc tgatctggat ctcggcctgg actacagcag ttccacccag
     4981 ctcagcgtcc atgtgagcgt ggacttcgaa atggctgagt gagtcgctgc acgtttgcca
     5041 gccggcccgc accgaacccc atagtctttg ctgatgaagc gcatcgccga tcttcgggtc
     5101 tcgccggtct gcctaggtgg caacgtgttc ggctggaccg cggacagtgc ggcgtccgag
     5161 cagatcctgg acgcctacgc ggacgcgggc cagaacttca tcgacactgc ggacgcgtat
     5221 tcggcctggg tgccgggcca ttccggcggt gagtccgaaa gcctgatcgg cggctggctg
     5281 cgcaagcggc ggctacgcga ccggatggtg atcgccacca aggtgggcaa gcacccgagc
     5341 cgccgcggac tgcgtccggc caacctgagg gtctgcctcg acggctcccg ccggcgcctt
     5401 ggcgtggaga caatcgacct ctactacgcc cactccgacg atgcgcatgt gccggtggcc
     5461 gaatgggtgg gtgcgttcga cgcgatgatc gccgatggcg cgatccgcca ctacggcttg
     5521 tccaacttct cggccgagcg caccactgag gtgttggaag tcgccaaggc cgaaggcctg
     5581 gctgcgccgg tggtcctcca gccgcagtac cacctggtcc atcgcagcga agtggaggca
     5641 ggcgggctgg ccgagttggc ggctgccaac ggcttggcga tcgtgcccta ctacgggctc
     5701 gccgccggct tcctcaccgg caagtaccgg gccgacgagc cgatgcacgg cgctcgggca
     5761 gcgcgggtga agcactgggc caacgcggag ggcttcgccg tggtggacgc ggtggtggac
     5821 atcgcgaacg atctggacgt cgaaccggcc accgtggccc tggcctggct acgacagcag
     5881 ccgggagtga ccgcgcccat cgcttcggtc agtacggtcg agcagttgcc ggcggttctg
     5941 gccgcgggcg aactcaagct cacccgcggc caactgaacc gcctgaacaa ggtgtcggcg
     6001 ccgttcgcct gagagctcag ctctcgactg agccttcgat ctgggtaggc ccgccgctgt
     6061 gggtgacgct gatcttgcgc ggctttgcct cctctgcgac cgggatcttc aggttcagga
     6121 caccgtcgct gtattcggcc gaaatccggt cgagcgccac gccgtagccg agggtcagct
     6181 gccgggcgaa ggttccggcc gggcgctccc ggaccagcca cttcgcgtcc gcaccggtct
     6241 ccgcacgccg ctcggcgcgg acggtgaggg tgcgatcgtc gacgtcgatg tcgatggacg
     6301 acgggtcaac cccaggaagg tcgatccggg ccacgaagac gtcgccgtcg cggtagaggt
     6361 ccatcggcat agcggtgctg gccggcgtcc ggggcgcttc ggagaaccag cgatcgaggt
     6421 cgcggaacgg gtcgtaacta cgtgccattg cactcactcc ttcgtctgtt gttagcactc
     6481 gccatgcccg agtgctaagt ccagtttgcg ccaaatccgg atgcatttca agcaagttga
     6541 gccacgtcgg ctcaactcga gttcgctgct cgcgaacgcc tcgaggagcg tggcttgact
     6601 agggtgtgtc cgtgcctcgc cagttcgtcg aacccagccc gcatccgtcc ggcgctcgcc
     6661 cgccgcaact cgacccgaag ctgctgcccc agcacgtggc gatcgtgatg gacgggaacg
     6721 gacgctgggc gaaggcccgc aatctcccgc gcaccgaagg ccacacccgc ggcgaggcgg
     6781 cgctcttcga cgtgatcgag ggcgccatcg agctgggcat caagtacctg tcggcctatg
     6841 cgttctccac cgagaactgg cgtcgctccc ccgacgaggt gcgctggctg atgggcttca
     6901 accgggacgt gatccatcgg cggcgcgacc agttcgtgga gatgggcgtc cgcgtccgct
     6961 gggccggacg cacgcccaag ctgtggcgca gcgtgatcaa cgaactgcag gaagccgagc
     7021 ggatgagcgc tggcaacgac cggatcacgc tgcagttctg cgtgaactac ggcggtcgcg
     7081 ccgagatcgt ggacgctgct cgcgctatcg ccgcggccgc ccagcgcggc gagctcgatc
     7141 cgtcccggct caccgaggac cgcttccggc gcttcctgga cgagccggag atcccggacg
     7201 tggacctgtt cgtgcgctcg tctggggaac agcgcacgtc caacttcctg ctgtggcagt
     7261 ccgcgtacgc cgaattggtc ttcctggacc ggctctggcc cgacttcgac cgccgcgact
     7321 tgtggcgcgc ggtggagatg tacgtgcagc gcgaccgccg ctacggcggt gccgtgccca
     7381 acgtgctcag ctagccaccc gaacctcacc acccgcaaac gcgacttcgc tcccgatagg
     7441 gacccgcgct cccgaattat cgggagcagg agttcctttc gggagcgaag ttgaagctgt
     7501 ggcccgggaa cggacgactc agtcggtgcc ggcctccata cccgaacggg tctgggcggc
     7561 ttcaatctcg gaatcggtgc cttcaccggc tgcgatcgcg ggcgccgtcc cagcttcgag
     7621 ctgaccaacc agggcagaga cctcaccgcc gaccagaccg agctgggcgt actgctccag
     7681 ccgggcacgg gagtcggcga tgtcgaggtt gcgcattgtg agctgaccga tgcggtccag
     7741 cgggccgaag gccgcgtcgc cgacgcgctc catcgagagt cgctccgggt ggtatgagag
     7801 cgacggcccg gcggtgtcca ggatcgagta gtcgtcgccg cgacgcagcc gcagggtcac
     7861 cgaaccggtc actgcggaac cgacccagcg ctgcagcgat tcgcggatca tcagcgactg
     7921 cgggtcgagc cagcgaccct cgtacatcag ccggccgagc ttgcggccct caagttggta
     7981 gctggcgaag gtgtcctcgt tgtggatggc ggtgaccagg cgctcgtagc agatgtgcag
     8041 cagcgccata cccggcgcct cgtagatgcc gcggctcttg gcctcgatga tccggttctc
     8101 gatctggtcg ctcatgccga gtccgtgccg gccgccgatc gtgttggctt cgcggacgag
     8161 gtcgacggcg gtggcgaagg tgcgtccgtt gatttgggtc ggacggccct gctcgaaggt
     8221 gatcgtgacg tcttcggtgg cgatctccac gctcggatcc cagaaccgga cgcccatgat
     8281 cggctcgacg atctcgatgc cgacattgag gttctcgagt tccttggctt cgtgcgtggc
     8341 gccccagatg ttggcgtcgg tggagtacgc cttctccttg gagtcgcggt a
//
LOCUS       DAOPRQ010000091         8223 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_7275_length_8223_cov_11.909770, whole genome shotgun sequence.
ACCESSION   DAOPRQ010000091 DAOPRQ010000000
VERSION     DAOPRQ010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 8223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8223
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7275_length_8223_cov_11.909770"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(113..1792)
                     /gene="recN"
                     /locus_tag="PKJ61_10545"
     CDS             complement(113..1792)
                     /gene="recN"
                     /locus_tag="PKJ61_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017936226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HOC14301.1"
                     /translation="MLSELRIVDLGVIGEAVIEPGPGFTAVTGETGAGKTMIVTGLAL
                     LAGAKADPKLVRTTASRALVEGRWRLDEQRHLALAELDAELEQDEVLVARALSSSRSR
                     MTIGGAQVPLATGSGLVGEWVTIHGQSEQLRLGTAERQLEVLDRYAGSELATELAAYR
                     LDYSARLVALAELTELTTRSQERARELDVLRFGLDEIAKADPQPGEDATLAAESLRLQ
                     AVDDLRQAAHTALVALAGDEDDYDAADAMGLVGVARKALGMAVDSDPQLLPITAQLAD
                     ASAALADVASELASYSASLDADPVRLEWIAGRRAELAHLTRKYGETVDAVLSWSAEAA
                     ERVLTLDAGDTRIGELREVIAELDARLAASAGRLSGLRRTAADVLARLVADELKALAM
                     PKARLAFDLTPTELGPNGADQVSLLFAANPGSVPGPLGKVASGGELSRVRLALEVVLA
                     AQSSGDTFVFDEVDAGVGGQVALEIGRRLARLAEHSQVIVVTHLAQVAAFADRHYQVS
                     KADDGSVTTSGVRLVTDEDRLAVLATMMGGLDSSEVAIAHAEELLATVRAG"
     gene            complement(1782..2714)
                     /locus_tag="PKJ61_10550"
     CDS             complement(1782..2714)
                     /locus_tag="PKJ61_10550"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014847010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HOC14302.1"
                     /translation="MSNQRRLVAVTIHPLRPAALEAAAEFITGINAHGIGCLAHSEDL
                     DALAARVPGAGLGVLDADNGTACEVVVVFGGDGNILRAAEWALPRQVPVLGVNLGHVG
                     FLAELESSQVGHLIETVVSRSYQVEERMTIDTVVRAERGGEQLWSSFAINEVSVEKLA
                     RERMLEVLVRIDERPLSRWATDGVLVATPTGSTAYAFSAHGPVIWPELSALLLVPLSA
                     HALFNRPLVLSDRTVVSIEVLPGGSPGGVVWCDGRRSTDVTGGMEIEARVGQHRLRLA
                     RTSEQPFVHRLVRKFGLPVSGWRGAAERDATHAQ"
     gene            complement(2724..3461)
                     /locus_tag="PKJ61_10555"
     CDS             complement(2724..3461)
                     /locus_tag="PKJ61_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004810921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HOC14303.1"
                     /translation="MRLDRALVERGLARSRTVAQAAIVSGAVTVNGEPATKPSQLVGE
                     ADLVAAQVDPWVSRAAHKLLGALADSGVEVGGRVLDAGASTGGFTEVCLARGAERVYA
                     VDVGHGQLASGLRSDPRVVVWERTNLRDLTLAHVEGEPVDLIVGDVSFISLRLLLRPL
                     LAVLRPSGSALLLVKPQFEVGRAGLDDHGVVRSAALRTQAVTAVIQTAGELGWRADWH
                     GESSLPGESGNVEYFVHFVSADVSAGG"
     gene            complement(3461..3616)
                     /locus_tag="PKJ61_10560"
     CDS             complement(3461..3616)
                     /locus_tag="PKJ61_10560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14304.1"
                     /translation="MTETEPTPPPQTGVPQVDEALDQLVLGEDVSTHPAALAAAVEAL
                     QRALNQS"
     gene            complement(3645..4145)
                     /locus_tag="PKJ61_10565"
     CDS             complement(3645..4145)
                     /locus_tag="PKJ61_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007079418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HOC14305.1"
                     /translation="MGNSRQDVVTAALRLLDAFGLDSFSMRRLAAELEVQPSALYHHV
                     ENKQELLGHLAGRIVGGVEVDADLMATCHRLRDAMLAVRDGAEVVATATAFRLGVSEF
                     EAELARFSSVDVARTLLIYVIGHTQATQLHRQASELGAIRTDPDLEASFDRGLRIILA
                     GQSPES"
     gene            4221..4814
                     /locus_tag="PKJ61_10570"
     CDS             4221..4814
                     /locus_tag="PKJ61_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015225 - biotin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015878 - biotin transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HOC14306.1"
                     /translation="MARPAAAPGADLALIAVFAALIAVFSLTPIPAGVIGVPITLQTL
                     AVALAGLVLGAWRGFLATLLYVVVGLAGLPVLAGGAAGIGVLAGPTAGYLLSFPLAAA
                     ATGAIAAHGLRRPSRFHYLWLGAAAIAGAVLVTTPLGIAGLMVNAGLPLSTATVVALG
                     FVPLDLVKMLVAAAIALAVHKAFPALLASRAAVVTEQ"
     gene            4889..5506
                     /locus_tag="PKJ61_10575"
     CDS             4889..5506
                     /locus_tag="PKJ61_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015582529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOC14307.1"
                     /translation="MDLELSERRIAFIGANGSGKSTLLRLLNGLVLPTTGRVLVGDLD
                     TRRDGRRIRRQVGFVFTDPLAQLVMATPADDVELSLRNTIPDRADRRAGAARLLAQRG
                     LAHLADQSVYDLSGGERQLVALTSVLACGPAIVVADEPTTLLDLRNRNQVRTAISELD
                     QQVLVATHDLEMAADADRVIVIDDGLVVADGEPASAIAAYRKLMA"
     gene            5503..6111
                     /locus_tag="PKJ61_10580"
     CDS             5503..6111
                     /locus_tag="PKJ61_10580"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HOC14308.1"
                     /translation="MNLFGLYVPGRSLFHRLGAGPKYLIMILLTVPAAVLRDWRLGLA
                     CLVAAMGLLAACRIGLARAWRLPAMVWVLVAMLLGYHLLSGTPGRGVNAAAVFLVAIY
                     VSRLLTSTTPGGELIDALIAGLRPLAVFGISAERVGLAVAVMLRSIPVLLDSFGQVRQ
                     AAMARGREHRLFALVTPVVVRAVGHAQATGAALAARGLGERE"
     assembly_gap    6335..6434
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6435..6958)
                     /locus_tag="PKJ61_10585"
     CDS             complement(<6435..6958)
                     /locus_tag="PKJ61_10585"
                     /inference="COORDINATES: protein motif:HMM:NF024740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HOC14309.1"
                     /translation="MTALIDAYDAALFDLDGVIYLGPLAVPGATVALAELQQRGTKVV
                     YVTNNAARAAETVVNQLRELGFDAGSENVLTSAQVAATALADVLAPGSKVLVAGSASL
                     SGLIAEVGLRPVSSAEDEPVAVLQGYDPMLSWPTLDEVCLAIARGASWYATNDDASRP
                     TDRGLVPGAGAMIAA"
     gene            complement(6955..7764)
                     /locus_tag="PKJ61_10590"
     CDS             complement(6955..7764)
                     /locus_tag="PKJ61_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015070784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOC14310.1"
                     /translation="MRQTPRPGLAAKANEPETPKGLDLRQLPRGVRAELRSLTSEHAE
                     VVGAHLLMAGQLIDTDPELAYAHAEAARRRAARLPITREATAETAYAAGDFNTALSEF
                     RALRRMTGGNEYLAAMADCERALGRHQAALKLVKEGLASQPDAAAEIELRLVEAGVRA
                     DSGQTAEALRLLKAGIESIGTRGPKVARARLRYGYADLLERTGDADQAERWFDAVLRL
                     DPDGTTDAEDRLAAIRGIVIEYDETEEDLFDEADESVSLDPETAETEPEQQ"
BASE COUNT         1212 a         2954 c         2696 g         1261 t
ORIGIN      
        1 gaccggtgcg ggatcccgac cctcgccggg aagaccggtg cgggatcccg accctcgccg
       61 ggatgagggg tgcgggtggg tcagcaactc gggcctgagc ctgacgaagg actcagccgg
      121 cgcgaaccgt tgccagcaac tcctcggcgt gggcgatcgc cacttcactg gagtccaggc
      181 cgcccatcat ggtggccagc acggctagcc ggtcctcatc ggtgaccaag cgcacgccgc
      241 tggtggtcac cgagccgtcg tcggccttgc tgacctggta gtgccggtcg gcgaaggcgg
      301 cgacttgggc caagtgggtg acgacgatca cctggctgtg ctcggccagc cgcgccaacc
      361 ggcggccgat ctcgagcgcg acctggccgc cgacccccgc gtccacctcg tcgaagacga
      421 acgtgtcgcc ggacgactgt gccgccagca cgacctcaag cgccagccgc acccggctca
      481 actcaccacc ggacgcgacc ttgcccagcg gcccgggcac gctgccaggg ttcgccgcga
      541 acagcaggct gacctgatcg gcgccgttcg gccccagttc agtcggggtg agatcgaacg
      601 cgagccgagc tttcggcatg gccagcgcct tcaactcatc ggccacgagc cgggccagaa
      661 cgtctgcagc cgtacggcgc agcccgctca accggccggc ggacgcagcc agccgcgcgt
      721 ccagctcggc gatcacctcg cggagctcgc cgatccgggt atcccccgcg tccagggtca
      781 gcacgcgttc ggccgcttca gccgaccaag acagcaccgc gtcaacggtc tcgccgtact
      841 tccgggtgag atgggccaac tccgcgcgtc ggccggcgat ccactccagt cgtacggggt
      901 cggcgtccag gctcgccgaa taggacgcca gctctgaggc cacatccgcc agcgcggccg
      961 aggcgtcagc gagctgcgcg gtgatcggca gcaactgcgg gtcggagtcg acggccatcc
     1021 cgagcgcctt gcgcgcgact ccgacgagac ccatcgcatc ggccgcgtcg tagtcgtcct
     1081 cgtcgccggc aagggcgacc agcgcggtgt gcgcggcctg gcggaggtcg tccacggcct
     1141 gcagccgcag cgactcagcc gccagcgtcg catcctcgcc gggctgcgga tccgccttcg
     1201 cgatctcgtc caacccgaac cgaagcacat cgagttcgcg cgcacgttcc tgcgacctcg
     1261 tggtgagctc ggtgagttcg gccagcgcaa ccaatcgcgc ggagtagtcc aaccggtaag
     1321 ccgccaactc ggtggccagt tccgacccgg catagcgatc gagcacttca agctgccgct
     1381 cagcggtgcc aagccgcaac tgctcggatt ggccatggat ggtcacccac tcacccacca
     1441 gtcccgagcc ggtggccaac ggcacctgcg cgccgccgat cgtcatccgc gaccggctgc
     1501 tgctcagcgc ccgggccacc agaacttcgt cctgctcaag ttcagcgtcc agctcggcga
     1561 gtgctaaatg ccgttgctcg tccaagcgcc aacgaccctc caccagggcc cgggacgcag
     1621 tcgtgcgcac cagcttcgga tcggccttcg ccccggccag cagtgccagg ccggtcacga
     1681 tcatggtctt gccggcaccg gtctcaccgg tgaccgcggt gaagccaggc ccgggttcga
     1741 tcacggcctc gccgatcacg ccgaggtcga cgatccgcag ctcactgagc atgcgttgcg
     1801 tcccgttccg cagccccccg ccagccgctc accggcaggc cgaacttgcg caccaggcgg
     1861 tgcacgaacg gctgctccga cgtccgggcc agtcggagcc gatgctgccc cacccgcgcc
     1921 tcgatctcca tgccgccggt cacgtcggtg ctccgccgac catcgcacca gaccacgccc
     1981 ccggggctgc cgccgggaag cacttcgatc gagacgaccg tccgatcgct gagcaccagc
     2041 ggacggttga acagcgcgtg ggcgctcagc ggcaccagca gcagcgcaga caactccggc
     2101 cagatcaccg ggccgtgcgc ggaaaaggca taggcggtcg acccggttgg cgtggccacc
     2161 aggacgccgt cggtcgccca gcgcgacagc ggtcgctcgt cgatgcgcac cagcacctcg
     2221 agcatgcgtt cacgggccag cttctcgacc gagacctcgt tgatcgcgaa cgagctccac
     2281 agctgctccc caccccgctc agcacgcacc acggtgtcga tggtcatgcg ctcctcgacc
     2341 tgatagctgc gcgagaccac ggtttcgatc aggtggccaa cctgggatga ctccagttcg
     2401 gcgaggaagc cgacgtggcc caggttcact cccagcaccg gcacctgccg cggcagcgcc
     2461 cattcggcgg cgcggaggat gttgccgtcg ccgccgaaga ccacgaccac ctcacaagca
     2521 gtgccgttgt ccgcatcgag tacgccgaga cctgcgccgg ggacgcgggc ggcgagcgcg
     2581 tcgagatcct cggaatgcgc caggcaaccg atgccgtgcg cgttgatgcc cgtgatgaac
     2641 tcggccgcgg cttcgagcgc agccggacgc agcggatgga tggtcacggc cactagccga
     2701 cgctggttgg acacgtgcaa accctagccg ccagcactga catcggcgga aacgaagtgc
     2761 acgaagtatt cgacgttgcc cgactccccg gggaggctgc tctcaccatg ccagtccgct
     2821 cgccagccca actcgcccgc cgtctggatc accgccgtca cagcttgcgt ccgcagcgcg
     2881 gcgctgcgca ccacgccgtg atcgtccagt ccggctctgc ccacctcgaa ctgcggcttc
     2941 accagcagca atgccgaacc ggacggccgc agcaccgcca acagtggacg cagcaacagc
     3001 cgcagcgaga tgaacgagac atcgccgacg atcaggtcga ccggctcgcc ttcgacgtgc
     3061 gccaaggtta ggtcgcgcag gttcgtccgc tcccagacca ccacccgtgg atcactgcgc
     3121 aagccggacg cgagctgccc gtggccgacg tcgacggcgt acacgcgctc ggctcccctg
     3181 gccaggcaca cctcggtgaa tccgccggtg gatgcgccgg cgtccagcac ccgtccgccc
     3241 acctcgacgc ctgaatccgc gagcgcgccg agcagcttgt gggcggcccg cgacacccac
     3301 ggatcgacct gtgcggccac aaggtcagct tcgccgacca gttgcgaggg cttggttgcc
     3361 ggctcgccgt tgacagtgac cgccccggac acgatcgcgg cctgcgcaac ggtacgggag
     3421 cgggccagac cccgttccac gagcgcgcga tccagacgga tcacgactgg ttgagcgcgc
     3481 gctgcagtgc ctcgacagcg gccgccaacg cggccggatg cgtgctgacg tcctcaccca
     3541 gaaccagttg gtccagcgcc tcgtcgacct gcggaactcc ggtctgcggc gggggtgtcg
     3601 gctcagtctc ggtcacccgc acatcgtagg ccctgctcat ggtgtcagga ctccggcgac
     3661 tgcccagcca ggatgatccg caggccacga tcgaacgacg cctccaggtc ggggtcggtc
     3721 ctgatggcgc ccagctcgct ggcttggcga tgcagctggg tggcctgggt atggccgatc
     3781 acgtagatga gcagcgtccg ggccacgtcc accgagctga aacgggccaa ctcggcctcg
     3841 aactcggaca cccccagccg gaaggcggtc gcggtggcca ccacctcggc accatcccgg
     3901 actgccagca tcgcatcgcg aagccgatgg caggtcgcca tcaggtccgc gtccacctcc
     3961 accccgccga cgatccgtcc cgccaaatgg ccaagcagtt cctgcttatt ctcgacatgg
     4021 tgataaaggg cgctgggctg cacctcgagt tcggcggcca gccggcgcat cgaaaaggag
     4081 tcgagcccga aggcatccaa cagtcgcagc gcagcggtga ccacgtcctg cctgctgtta
     4141 cccatggccg cagtctatgg gctaacctga acactgttca cctgaacacc gttcaggcag
     4201 tcaatctccg aggagttccc atggcccgcc ccgcagccgc gcccggcgcc gacctggccc
     4261 tgatcgcggt cttcgccgcg ctgatcgcgg tgttctcgct gactccgatc cccgccggcg
     4321 tgatcggcgt gccgatcacc ctgcagaccc tggcggtcgc gctggccggc ctcgtgctcg
     4381 gcgcctggcg cggcttcctc gccaccctgc tgtacgtagt cgtgggcctc gccggccttc
     4441 cggtgctcgc cgggggcgcc gccggcatcg gcgtgctcgc cggccccacc gccggctacc
     4501 tgttgagctt cccgctggcg gcagccgcca ccggggcaat cgcggcccac gggcttcgcc
     4561 ggccgagccg gttccactac ctgtggttag gggcagcggc catcgccggc gcagtcctgg
     4621 tcaccacgcc gctggggatc gccggcttga tggtcaacgc cggcctgccg ctgtcgaccg
     4681 cgactgtggt ggcgctgggc ttcgtcccgc tggacctggt gaagatgctg gtcgcggccg
     4741 cgatcgcgct ggccgtgcac aaggcctttc ccgcgctgct ggcgagccgg gctgcggtgg
     4801 tcaccgagca gtgatccgcc tcgacggggt tcaggttgcg gttccaggat cgggtgccgc
     4861 gcggacgcag cggcgcatcc tcggcccggt ggacctcgag ctcagcgagc gccggatcgc
     4921 cttcatcggc gccaacggtt cgggtaagtc cacgctgctg cggctgttga acgggctggt
     4981 cctgcccacc accggacggg tgctcgtcgg cgacctcgac accaggaggg acgggcgccg
     5041 gatccgccgc caggttgggt tcgtgttcac cgatcccctg gctcagttgg tgatggcgac
     5101 ccctgctgac gacgtcgaac tcagcctgcg caacaccatc cctgatcgag ccgatcgccg
     5161 cgccggcgcc gcccggctgc tggcacagcg cggcttggca cacttggccg accagagtgt
     5221 ctacgacctc tccggcggcg aacggcagct ggtcgcgctg acgtccgtgc tggcgtgcgg
     5281 acccgcgatc gtggtcgcgg acgaacccac caccctgctc gacctgcgaa accgcaacca
     5341 ggtgcgcacc gcgatcagcg agctcgacca gcaggtgctg gtcgccaccc acgacctgga
     5401 gatggccgcc gatgccgacc gggtgatcgt gatcgacgac ggcctggtgg ttgcggatgg
     5461 cgaaccggcg tccgccatag cggcgtaccg gaagctgatg gcatgaacct cttcgggctc
     5521 tacgtgcccg ggcggtcgct gttccaccgg ctcggcgccg gcccgaagta cctgatcatg
     5581 atcctgctga ccgtgccggc agcagtgctg cgcgactggc ggctgggatt ggcctgcctg
     5641 gtggcggcga tggggttgct ggcagcctgt cgtatcgggc tggcccgcgc gtggcggctg
     5701 cccgcgatgg tgtgggtcct ggtggcgatg ctgctgggct atcacctgtt gtccggcacc
     5761 ccgggtcgcg gggtcaacgc tgcggccgtg ttcctggtcg ctatctacgt cagtcggctg
     5821 ctgacgtcca cgacgcccgg cggcgagctg atcgacgcgc tgatcgccgg gctgcgtccg
     5881 ctggcggtct tcggcatctc agcagagcgt gtcggactgg cggtcgcggt gatgttgcgc
     5941 tcgattccgg tgctgctcga cagcttcggc caggtgcggc aggccgcgat ggccagggga
     6001 cgcgagcatc gactgttcgc gctggtgacg ccggtggtcg tgcgggcagt tggccacgcc
     6061 caggccaccg gagccgccct ggccgcccgc ggtctcggcg agcgcgaata ggccggacgc
     6121 ccgaggtcag ctgatcaggt cgagagcttc cagagcgggc gctgcgtcca agtagggctc
     6181 cgaccatgct ttcgcggcca ccgcccacaa cgcatcgaac tgctcagaca cggttcgcgg
     6241 gtgggtctgg agaacaatcc ggctgtcatc ctgcgcagca cgctggccat tgcattcagc
     6301 gtccgactct gtgagcgtca cccgtcgtga cggcnnnnnn nnnnnnnnnn nnnnnnnnnn
     6361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6421 nnnnnnnnnn nnnngcggcg atcatcgcac cggcgccggg caccaggccc cgatcggtcg
     6481 gacgcgaggc atcgtcgttc gtggcgtacc agctcgcgcc acgtgcgatc gcaaggcaga
     6541 cttcgtccag cgtcggccag gacagcatgg ggtcgtagcc ctgcagcacc gcaaccggct
     6601 cgtcctccgc actggacacc ggtcgcaggc cgacctcggc gatcaggccg gacagactcg
     6661 cactacctgc caccagcacc ttggaacccg gggcgagcac gtcggccagc gcggtcgccg
     6721 cgacctgggc gctggtcagc acgttctccg aaccggcgtc gaagcccagt tcccgcagct
     6781 ggttgaccac cgtctccgca gcgcgcgcgg cgttgttggt gacgtacaca accttggtgc
     6841 cgcgctgctg caactcggcc agtgcaaccg tggctcccgg caccgcgagt gggccgaggt
     6901 agatcacccc gtccaagtcg aacagggctg cgtcgtaggc gtcgatcagc gcggtcactg
     6961 ttgttccggc tccgtctcgg cggtctcggg atccaggctc accgactcgt ccgcttcgtc
     7021 gaacaggtct tcttcggtct cgtcgtactc gatcacgatg ccacgaatgg ccgcgagccg
     7081 gtcttcggcg tcggtggtgc cgtccgggtc gagacgcagg acagcgtcga accaccgctc
     7141 ggcctgatca gcatcaccgg tgcgctccag caaatcggcg tagccgtagc gaagccgtgc
     7201 ccgagccacc ttcgggcccc gggtaccgat gctctcgata ccggccttca gtagccggag
     7261 cgcttcagcg gtctggcccg agtcggcgcg gacgccggct tcgaccagac gcagctcgat
     7321 ctcggcggcc gcgtccggct ggctggcgag gccttccttg acgagcttga gcgctgcctg
     7381 gtgccgaccg agggcacgct cgcaatcggc catggccgcc aggtactcgt tgccgccggt
     7441 catccgacgc agcgcacgga actcgctcag tgccgtgttg aagtcaccgg cggcataagc
     7501 ggtttccgcc gtcgcctctc tggtgatcgg cagtctggcc gcccggcgac gggccgcttc
     7561 ggcgtgcgcg tacgccagtt ccggatcggt gtcgatgagc tgtccagcca tcagcaggtg
     7621 cgcacccacc acctcagcgt gctcgctggt caagctgcgc agctcggcac ggacgccgcg
     7681 cggcagctgc cgaaggtcga ggcccttggg tgtctccggc tcgttggcct tggcggccag
     7741 gccgggacgt ggtgtctgcc gcacttggtc acgatcgtcg cgctggtagg caggccgact
     7801 tgacgagcgt ggacggtcag cgttgggccg tccatcccgg cgaccgtcac gaggacgctc
     7861 accacctcgg ggaccggcgg gtcgatcgcc acggctaccg gacgggcggc cacttgtgcc
     7921 gctaccgccc ggacccgacg accacgactt cttcggcttg tcggtcgacc agtccttgcg
     7981 tgggcgatca tcccgagatc ctgcggacga tccgtcgcgg cgggcacgat cgaagtcccg
     8041 gtccttgcgg ggccgctcgc tcccagaccg atcgtcgcga tttcggtcct gccagggacg
     8101 atcatcgcgc ctggcatcgc ctgagcgccc gccgctcttc gccttgcccg cgccgcggtc
     8161 gtagccaggc cggtctggcg tccaatcctt gcgtggccgg tcgtcacggg gacggtcacc
     8221 gcg
//
LOCUS       DAOPRQ010000092         8106 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_7395_length_8106_cov_12.505527, whole genome shotgun sequence.
ACCESSION   DAOPRQ010000092 DAOPRQ010000000
VERSION     DAOPRQ010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 8106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8106
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7395_length_8106_cov_12.505527"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2734)
                     /locus_tag="PKJ61_10595"
     CDS             complement(<1..2734)
                     /locus_tag="PKJ61_10595"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOC14311.1"
                     /translation="MKLIRGLGALSVLLVVLVGAPLALLTGGRYPSGWSGLLRPDDGS
                     MWLVVLTAVGWLAWVAFTAATLVEAVQLVSGVRFRLPLLGGLQQVSAGLLLAVLSLAP
                     ASTAVSPSAAVVVAAVPQTGPDADSLDADPVGDLLPAPAAEADWYVVASGDDLWSLSE
                     SLLGDGSRWRELVAANPETLAEPTARLTAGTRLVLPASALAPRQVVVERGDTLSGLAL
                     EHLGTAKRWPAIAAANADLIEDPDHIEIGWRLDLPAAHGSRSSDARPERDPAPAHEPD
                     DEPSADRGTGEGPNARSGPEPPSTTPPELDQGPVAATAQAEAPAEVSDDQDLPAIGVI
                     GSLAAAALIGTLEARRYVRLRSRPVGRRLISPTEPETRLRTAIGRRQRPDGTKLLDAA
                     LRTIGEHCFTNTRQLPALERATLGETTVSFQWVHPAGAPPSGFEGDDRSWRVEFSAAT
                     LRAPDWPCPYPALVSLGETASGDTLLVDAERSRVLGVAAATDELRRSALAAMALELTC
                     APWSRELRLIAAGDEAAMLALAGGDRVEVAPSINEAIARLRCAVADRRRALAGEPLAR
                     LRVDPDRADAVAPLVLVLNTRVDAALAEELDELLAGPATGAAVLLGSDSGAPALWQVS
                     GEEYAPSGQLTGSPGRLLAHSIPAATRAGVASLLRAADDPADTPAPWWGDPTDNVRPL
                     PQRANRGEEAMDIVRLLPVADAPRALLIGPVDLLGAAGPAPVRSRQQLVEACAWLLEN
                     PGRSATEMSLGLGVAEGTRRSNLSRLRAWLGSDPAGKPYLPDAYSGRIRLHPAVTSDW
                     ESVQALLRPGIDRVSDATLVAALELVRGAPLADVAPGLWHWAEELRTDLSSALRDVGL
                     VLADRALANGDVDLARWAASRALVVAPEDEQLLCARIRTEHRAGNRAEVERLVNQ"
     gene            complement(2731..3129)
                     /locus_tag="PKJ61_10600"
     CDS             complement(2731..3129)
                     /locus_tag="PKJ61_10600"
                     /inference="COORDINATES: protein motif:HMM:NF024792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HOC14312.1"
                     /translation="MAIEFVLLVPVLVLLFGVVVGGARVWLARGVAEQAAAAAARAAS
                     GERSVAKAERAGRELAVAQASTSGLRCAEFSVLVDADALAQDAGTPGQVHATVSCAVP
                     LGDVLVPGWPGTVDVSGDATAVVDRYRGRK"
     gene            complement(3156..3515)
                     /locus_tag="PKJ61_10605"
     CDS             complement(3156..3515)
                     /locus_tag="PKJ61_10605"
                     /inference="COORDINATES: protein motif:HMM:NF019428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HOC14313.1"
                     /translation="MNERGLAESTQWAVLAPTLLGLVLGLVQLGVWLHSRTIAASAAA
                     AVADVRALGASQDAAAREVGRRVAIAGGLQDVDIQLSTGADMVLVTVSGRAPLFFDLG
                     QGRVAETAVLPREVVKP"
     gene            complement(3512..3688)
                     /locus_tag="PKJ61_10610"
     CDS             complement(3512..3688)
                     /locus_tag="PKJ61_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002550924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14314.1"
                     /translation="MRFLHLLVAALVAPPPRRDERGLSQSTENAILLAGAVAVALIVV
                     TAVTVYVSAKLPRP"
     gene            complement(3710..4591)
                     /locus_tag="PKJ61_10615"
     CDS             complement(3710..4591)
                     /locus_tag="PKJ61_10615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14315.1"
                     /translation="MNSLLVAIGLGALIGFGLWSVARAFALPRPRLADALDLLDGSVQ
                     VEALPETTGLDRLGTWLRAQSRQPASASTLRRLKLAGRSLDRHYAYKGIWALLGLLLP
                     TLCGAVLALMTGAGLLLPAGLGLVCSAIGFVLPDVLLRQAETEVAADATEALLTYFDL
                     VTLERLANQSGTQSLRSAAALSDSTVFASIRDALERARLEQRAPYGELKRLGAELDLP
                     ALVDIADVMALDEAGATLSGALRARVRELRDAHLTEARIAASAVSERMALFMVIPSLV
                     FGLFFLVPPILRLIGAA"
     gene            complement(4588..5448)
                     /locus_tag="PKJ61_10620"
     CDS             complement(4588..5448)
                     /locus_tag="PKJ61_10620"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOC14316.1"
                     /translation="MGTFLASLCGLLIAGGLMLVIAGFNSPGAQRAASSTGLWKPLVE
                     SGVTNAARRHWWRLLLAVGAGLLAAGYTGWPLLAVLVPLVVNVLPMLLSAPPNRELAL
                     LEALDRWVRTLSSLLPTGRSITDAIRLSGRQAPTLIAPQLRLLAARLDDRWTIQQALR
                     ALADELNSPDADAVLAALSLAAQRGGTGAAATLAALADNVQDRLRALREIETERAKPR
                     IVVRQVTGVTVVVLALAFVFGGQFFAPYASGLGQALLALLVAAYLGSLLFLRRLTTPR
                     PRQRILSSAP"
     gene            complement(5448..6833)
                     /locus_tag="PKJ61_10625"
     CDS             complement(5448..6833)
                     /locus_tag="PKJ61_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF/VirB11 family protein"
                     /protein_id="HOC14317.1"
                     /translation="MKRLLDVELLAEPVEFETVFTRERLIAERPEEPMVDWQLVVALR
                     RKASERITTALSERGQLTEVDRRLLGRSLVRQVVRDHVDGLSTRGEALWPMAVEDAYV
                     QATESAIFGYGRLQPLFEIPEAENVEIHGFDSVVVQYGDGRREPRPPVADSDEELVDA
                     IRFLGESVNPARPFDDAHPMMTLALGDRFRLHAIGFGLSFRPSITIRQHTLTDVTLAD
                     LAAGGMMPLEVARFLHAAVLARKSMVISGDQGAGKTTLLRALIAAIPANERFGTLETD
                     YELLTHLQPNRRNMLALQARVGMGEIQDGRRVGEFTVADLIPEALRQNLSRLIVGEVR
                     GGEAGAMFEAATSGAGLISTTHSHSATSAIDRLASRVAQGGVLTVEEAYRQVAHHIHL
                     LIHVRLTDDTWRGGVRTRAITEIRQLTGGLEGGRPVTHLAYRSGDADGNFCPDADLIA
                     ELAQFYAAWDA"
     gene            complement(6830..7591)
                     /locus_tag="PKJ61_10630"
     CDS             complement(6830..7591)
                     /locus_tag="PKJ61_10630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14318.1"
                     /translation="MNTWLFTSAGHASGVTALTLALATLWPDDVLLVDGNREPDQSVL
                     AGYLQGANPAGRGLGAVLQAHRERRPLAECLPGLTLPLGDASSSDFLPGFAHPGMVAL
                     FAQVWPDFAAALSASERTVLVDAGRIGPTGLPLPMVSVSSGVVVVTGSRLVDLAALRL
                     YLPLVVASAGEERVGLVVVGPNRPYGTGEISERFGVPVWGKVAWSPAEAAVFAAGERA
                     PRRRLVGPYLADVRALAVSLLEQTAVHRSLIGVPR"
     gene            complement(7588..>8106)
                     /locus_tag="PKJ61_10635"
     CDS             complement(7588..>8106)
                     /locus_tag="PKJ61_10635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14319.1"
                     /translation="ALLYADISNSISAVAVVRTVYRDQVITEADLGITSAVPAAGLEL
                     VPSEQLTAIIGRTAQLDLTQGTLLSPRSFGDPATEPGHVRLGLRLAAGRIPAAPLPPG
                     ARVLLVPVGRDGADPPSGASVVGRVATAASTLPDGAALVDVSIAEAEAERVARLAAAD
                     QVVLVQLPGVPR"
BASE COUNT         1316 a         2922 c         2785 g         1083 t
ORIGIN      
        1 cctgattcac cagccgctcc acctcggcgc ggttgccggc ccgatgctcg gtacggatcc
       61 gcgcacagag cagctgctcg tcctcgggcg cgacgaccag ggctcgggac gcggcccacc
      121 gggccaggtc gacatcaccg ttggcgagcg cgcgatcggc cagcaccagc ccgacgtcgc
      181 gcagcgcgga cgacagatcg gtgcgtagct cctccgccca atgccagagc cccggcgcaa
      241 cgtcggccag tggtgcgccg cggacgagtt cgagcgccgc caccaatgtg gcgtcgctga
      301 cccggtcgat gcctggacgc agcaacgcct gcaccgactc ccaatccgaa gtcaccgcag
      361 ggtgcagccg gattcgcccg gagtaggcgt ccggcaggta cggcttcccg gccgggtcgc
      421 tgccgagcca ggcgcgcagc cggctcaggt tggagcggcg ggtgccctcg gcaacgccca
      481 gccccagcga catctcggtc gcgctgcggc cagggttctc gagcagccag gcacacgcct
      541 cgacgagttg ttgccgcgag cggacgggag ccgggccggc ggcaccgagc agatcgaccg
      601 gcccgatcag gagcgcccgc ggcgcgtccg ccaccggcag gagccgcacg atgtccattg
      661 cttcctctcc tcgattggct cgttgaggca acggccggac gttgtcggtc gggtcgcccc
      721 accatggcgc gggggtgtcc gccggatcgt cggcggcccg caacagggac gccacgccag
      781 cccgggtggc cgcgggaatg ctgtgcgcga gcagccgtcc gggcgatccg gttagctggc
      841 ccgacggcgc atactcctcg ccgctgacct gccacagcgc gggcgcgccg gagtcgctgc
      901 ccagcagcac ggcggcaccg gtggccgggc cggcgagcaa ctcgtccagc tcttcggcca
      961 gggccgcgtc cactcgcgtg ttcaggacga gtaccagggg cgcgacggcg tccgcgcggt
     1021 caggatcgac gcgcaggcgg gcgagcggct ctccggccag cgctcttcgc cgatcggcca
     1081 cggcacatcg caggcgggcg atcgcctcat tgatgctcgg cgccacttcc acccgatcgc
     1141 cgccggccag cgccagcatg gcagcctcgt cgccggctgc gatcagccgc agctcacggc
     1201 tccagggcgc gcaggtcagt tcgagcgcca tcgcggccag agcggatcgg cgcagttcgt
     1261 cggtagcggc ggcgaccccg agcacccgcg acctctcggc gtccaccagc aaggtgtcgc
     1321 cgctcgccgt ctcgcccagg ctgaccaatg ccgggtaggg gcacggccag tccggtgccc
     1381 gtagcgtggc ggcggagaac tcgactcgcc agcttcggtc gtcgccttcg aagccggacg
     1441 gcggcgcgcc cgccgggtgg acccactgga agctcaccgt ggtttccccg agtgtcgctc
     1501 gttccagcgc gggaagctgg cgggtgttgg tgaagcagtg ctcgccgatg gtgcgcagcg
     1561 ctgcgtcgag cagcttcgtg ccatccgggc gctggcgacg gccgatcgca gtgcgcagcc
     1621 gcgtttccgg ttcggtaggc gaaatcagtc ggcggcccac cggacgcgag cgcagccgca
     1681 cgtaccgccg cgcttccagc gtcccgatca gcgccgccgc ggctagggag ccgatcaccc
     1741 cgatcgccgg gagatcctgg tcatcgctga cttcagctgg cgcctcggct tgggcggttg
     1801 cggccaccgg cccttggtca agctcagggg gcgtggtcga aggtggctca ggacccgatc
     1861 gggcattggg tccttcgcct gttcctcggt ccgcggacgg ctcgtcgtct ggctcatggg
     1921 ctggcgccgg gtcccgctca ggcctggcgt ccgaacttcg ggagccgtgc gcggcgggta
     1981 ggtcgagccg ccagccgatc tcgatgtggt ccgggtcttc gatcaggtcg gcgttggctg
     2041 cggcgatcgc cggccagcgt ttggctgtcc cgagatgctc aagggccaat ccgctgagcg
     2101 tgtcgccacg ttccaccact acctgcctgg gcgccaaagc ggacgcgggc agcaccaatc
     2161 gggtgccggc ggtgagccgg gcggtgggct cggccagggt ctcagggttg gccgccacca
     2221 actcccgcca acgggagccg tccccgagca acgactcgct gaggctccac aggtcgtccc
     2281 cactcgcgac cacataccag tccgcttcgg cagccggagc cggcagtaag tcgcccaccg
     2341 gatcggcatc gagggagtcc gcgtccggcc cggtctgggg cacggccgca acgaccactg
     2401 ctgccgacgg cgacaccgcc gtggacgcgg gagccagcga caggaccgcc agcagcaggc
     2461 ccgcgctcac ctgctgcaac ccgcccagca gcggtagccg gaaccgcacc cccgaaacga
     2521 gttgcacggc ctccaccagg gtcgctgcgg tgaaggccac ccaagccagc caaccgactg
     2581 cggtgagcac caccagccac atcgacccgt cgtccggacg cagcaacccc gaccagccgg
     2641 acgggtatcg tcccccagtc agcaacgcca ggggcgcgcc gacgagcacc accagcagta
     2701 ccgaaagggc acccaacccg cggatcagct tcatttccga cctcgatatc tgtcgacgac
     2761 agcggtcgcg tctccggaga cgtcgacggt ccccggccat cccggaacca gcacgtcgcc
     2821 cagcggcacc gcgcagctca ccgtggcgtg cacttggccg ggcgtccccg cgtcctgcgc
     2881 gagtgcgtcc gcgtccacca gcacgctgaa ctcggcacag cgcaggccgc tggtgctcgc
     2941 ctgcgccacg gcgagctcgc gtccggcccg ctcggctttg gccacgctgc gctcgccgga
     3001 agcggcccgg gctgctgcgg cagctgcctg ctcggcgacg cctctggcca gccagacccg
     3061 cgccccaccc accacaacgc cgaacaacag caccagcacg ggcaccagca ggacgaactc
     3121 gatcgccatt gagcccctct ggtcgcggcc gacggtcacg gcttcaccac ttccctcggc
     3181 agtaccgcgg tttcggcgac tctgccctgg ccgaggtcga agaacagcgg cgcccgtccg
     3241 gaaaccgtca ccagcaccat gtcagcgccg gtcgagagct ggatgtcgac gtcctgcagg
     3301 ccgccggcga tagcgacgcg gcggccgact tcgcgagccg cggcatcctg ggaagccccg
     3361 agcgcacgta cgtccgccac cgcggccgcg gcgctggccg cgatcgtccg cgagtgcagc
     3421 cagacaccga gttgcaccag tccgaggacc agaccgagca gcgtcggcgc gaggacggcc
     3481 cactgggtgg actcggccaa cccgcgctcg ttcacggtcg gggcagtttg gccgatacgt
     3541 agacggtcac cgcggtgacg acgatcaggg ccactgcgac cgcgcccgcc agcaggatgg
     3601 cgttctcggt cgactgggac aggccgcgct catcccgtcg cggcggcggc gccaccagtg
     3661 cagcgaccag caggtgcagg aatctcatta ctcctcctta gttgtggact caggcagcgc
     3721 cgatcaggcg caggatcggt ggcacgagga agaacaggcc gaacaccagc gatgggatga
     3781 ccatgaacag cgccatccgc tccgaaactg cggacgccgc gatcctcgcc tcggtgaggt
     3841 gcgcatcgcg aagttcgcgg acgcgagcgc gcagcgctcc tgaaagcgtc gccccggctt
     3901 cgtcgagggc catcacgtcg gcgatgtcga ccagcgcggg caggtccagt tccgcaccga
     3961 gccgtttcaa ctctccgtac ggtgcacgct gttcgagacg agcccgctcg agcgcgtccc
     4021 ggatggacgc aaaaacggtc gagtcgctca gcgccgctgc cgaccgcagc gactgtgtgc
     4081 cggactggtt ggccagacgc tccagcgtga ccaggtcgaa gtaggtcagc agcgcttcgg
     4141 tcgcatcggc tgccacttcg gtctcagcct ggcgcaggag cacgtcgggc agcacgaacc
     4201 cgatcgccga acacaccagg ccaagcccgg ctggcaggag taggccggcg ccggtcatca
     4261 gggccagcac ggcaccgcac agggtcggca gcagcaggcc gagcagtgcc cagatgccct
     4321 tgtaggcgta gtgccgatcg agactgcggc cggcaagctt cagccgacgc agggtgctag
     4381 cgctcgccgg ctgacgtgac tgggctcgca accaggtacc gagccggtcc agacccgtcg
     4441 tctccgggag tgcctcgacc tgaacgctgc cgtccagcag gtcgagcgcg tctgccagcc
     4501 ggggacgcgg cagcgcgaag gcccgggcca cgctccagag cccaaagccg atgagcgcac
     4561 ccagcccgat cgcgaccagg agcgaattca tggcgccgaa ctcaggatgc gttgccgcgg
     4621 acgcggcgtg gtcaggcgac gcaggaacag cagcgaaccg aggtaggcag ccaccaacaa
     4681 ggccagcaag gcctgcccga gcccggacgc gtagggcgcg aagaactggc cgccgaacac
     4741 aaaggccagc gccagcacca ccaccgtcac cccggtcacc tgacggacga cgattcgtgg
     4801 cttggcgcgc tcagtttcga tctctcgaag ggcgcgcagc cggtcctgca cgttgtcggc
     4861 cagtgcggcc agagtggcgg ctgcaccggt gccgccacgc tgcgcggcaa gggaaagcgc
     4921 ggccagaacc gcgtccgcgt ccgggctgtt cagttcgtcg gcgagcgcgc gcagggcctg
     4981 ctggatggtc cagcgatcat ccagccgggc ggcgaggagc cgcagttggg gagcgatcag
     5041 ggtgggcgcc tgccgtccgg acagccggat ggcgtcggtg atcgagcgac cggtcggcag
     5101 cagcgaactc agcgtccgca cccagcggtc gagtgcctcc agcagcgcga gttcacggtt
     5161 gggcggcgcc gagagcagca tcggcagcac gttgacgacc agcggaacca gcaccgccag
     5221 tagcggccag ccggtgtaac cggcagcaag tagaccagcg cccacggcca gcagcagccg
     5281 ccaccagtgc cgccgcgcgg cgttggtcac tccactttcc acaagcggtt tccacagacc
     5341 tgtggatgag gcggcgcgtt gagcgccagg gctgttgaaa cctgcgatca ccagcatcag
     5401 tccgccggcg atgagcaggc cgcacagcga tgcgaggaag gtgcccatca ggcgtcccag
     5461 gccgcgtaga actgggcaag ctcggcgatc aggtcggcgt ccgggcagaa gttcccgtcc
     5521 gcgtcgccgc tgcgataggc gaggtgcgtg accggacggc caccctccaa accacccgtg
     5581 agttgccgga tctcggtgat ggcccgggtc cggacaccgc cgcgccaggt gtcgtcggtc
     5641 agccggacgt ggatgagcag gtgaatgtga tgagccacct gacggtaggc ctcctcgacg
     5701 gtgagcaccc cgccctgggc gacccgggac gctagccggt cgatcgcgga agtggccgaa
     5761 tgcgagtgcg tcgtgctgat caggccggcg ccggaggtcg ctgcttcgaa catcgcgccg
     5821 gcctcaccac cgcgcacctc acccacgatc agccgggaga ggttctgccg gagcgcctct
     5881 ggaatcaggt cggcgacggt gaactcacca acccggcgtc cgtcttggat ctcgcccatc
     5941 ccgacgcgtg cctgcagcgc gagcatgttc cgccggttcg gttgcaggtg tgtgagcagc
     6001 tcgtagtcgg tttcgagcgt gccgaagcgc tcgttggccg ggatcgctgc gatcagggcc
     6061 cgcagcagcg tggtcttgcc ggccccttga tcgccggaga tcaccatcga cttgcgggcc
     6121 aacaccgcag catgcaggaa ccgcgcgacc tctagcggca tcatgccgcc ggcggccagg
     6181 tcggccagcg tgacgtcggt gagtgtgtgc tgccgaatcg ttatcgacgg ccgaaaactc
     6241 aacccgaagc cgatcgcgtg caaccgaaaa cggtcgccga gcgcgagcgt catcatcggg
     6301 tgcgcgtcgt cgaacggccg cgccgggttc accgattcgc cgaggaaccg gatggcgtcg
     6361 accagttcct cgtcgctgtc agccaccggc ggacggggct cgcgccgacc gtccccgtac
     6421 tggaccacga ccgaatcgaa gccgtggatc tcgacattct cggcctcggg tatctcgaac
     6481 agcggctgca accggccgta cccgaagatc gcgctctcgg tggcctgcac gtaggcatcc
     6541 tcgaccgcca tcggccacaa cgcttcgccg cgtgtgctca atccgtcgac gtggtcgcgc
     6601 accacttggc gcaccagcga tcggccgagc agacgccggt cgacctcagt caactggcca
     6661 cgctcggaca gggcagtggt gatccgctca gaagcctttc ggcgcagcgc caccaccaac
     6721 tgccagtcga ccatcggttc ttcgggccgt tcggcaatca gccgctccct ggtgaacacc
     6781 gtctcgaact cgaccggttc ggccagcaac tccacgtcca gtaggcgttt catcgcggca
     6841 ccccgatcag ggatcggtgg acggccgtct gctcgagcag ggacacagcc agcgctcgca
     6901 cgtcggccag gtaaggacct accaggcgac ggcggggcgc ccgctcaccg gcggcgaaca
     6961 ccgcggcctc ggccggcgac caggccacct tgccccacac cggcacgccg aagcgctcgc
     7021 tgatctcgcc ggtaccgtac ggccgattcg gtccgaccac aaccaggccc acccgctctt
     7081 cgcctgcgga cgccaccacc agcggcaggt acagccgcaa cgcggccaga tcgaccagcc
     7141 tggagccggt caccaccact acgccactgc tgaccgagac catcggcagc ggcaggccgg
     7201 tcggcccgat ccgcccggca tcgaccaaca cggtgcgctc ggacgcggac agtgcggcgg
     7261 cgaagtcagg ccacacctgc gcgaacaggg cgaccatgcc gggatgtgcg aagccaggca
     7321 ggaagtcgct actgctggcg tcgcccaggg gaagggtcag acccggcagg cattcggcca
     7381 gcggacggcg ttcgcggtgc gcctgcagca ccgcgcccag accgcgtccg gccgggttgg
     7441 cgccctgcag gtacccggcc agcaccgact ggtccggctc gcggttgccg tcgaccagca
     7501 gcacgtcgtc gggccacagg gtggcgagcg cgagcgtgag cgcggtcacc ccggacgcgt
     7561 ggccggcgct ggtgaacagc caggtgttca tcgtggcacc ccggggagct ggaccagtac
     7621 cacctgatct gcggccgcga gccgtgcgac ccgctccgcc tccgcctcgg cgatcgacac
     7681 gtccaccagg gccgcgccgt cgggaagggt ggacgcggca gtagcgacgc ggccgaccac
     7741 gctcgccccg gacggtgggt ccgcgccgtc ccgtccgacc gggaccagca atacccgggc
     7801 gcccggcggc agcggtgccg ccgggattcg accggcggcc agccgcagac cgagccggac
     7861 gtgtcctggc tcagtggccg ggtcgccgaa ggagcgcggg ctcaacaggg ttccctgagt
     7921 caggtcgagc tgcgcggtgc gtccgatgat ggccgttagc tgttcggacg gcacgagttc
     7981 gagcccggca gcgggcaccg ccgaggtgat gccgaggtcg gcttcggtga tcacctgatc
     8041 gcgatagacg gttctgacca ccgcaacagc ggaaatagag ttgctgatat ccgcgtagag
     8101 cagggc
//
LOCUS       DAOPRQ010000093         8103 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_7397_length_8103_cov_13.349279, whole genome shotgun sequence.
ACCESSION   DAOPRQ010000093 DAOPRQ010000000
VERSION     DAOPRQ010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 8103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8103
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7397_length_8103_cov_13.349279"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1895
                     /gene="scpA"
                     /locus_tag="PKJ61_10640"
     CDS             <1..1895
                     /gene="scpA"
                     /locus_tag="PKJ61_10640"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013160681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HOC14320.1"
                     /translation="NAFYRRNLAAGQKGLSIAFDLATHRGYDSDHPRVTGDVGMAGVA
                     VDSILDMRQLFDGIPLDQMSVSMTMNGAVLPILALYVVAAEEQGVKLEQLAGTIQNDI
                     LKEFMVRNTYIYPPAPSMRIIADIFAFTSANMPKFNSISISGYHMQEAGATADIEMAY
                     TLADGVEYIRAGEAVGLKVDQFAPRLSFFWAVGMNFFMEVAKFRAARLLWARLVRQFE
                     PKNPKSMSLRTHSQTSGWSLTAQDVFNNVGRTCLEAMAATQGHTQSLHTNALDEAIAL
                     PTDFSARIARNTQLFIQQESGTCRTVDPWAGSAYVERLTYELASKAWDLIQEVEAAGG
                     MAKAIEAGIPKMRIEEAAARTQARIDSGRQPLIGVNKYIVENDVLPEVLKVDNNSVRQ
                     QQIDKLTRLRAERDSAATQAALEKLTWAAENPDSTDPERNLLKLAIDAARAKASVGEI
                     SDALEKVFGRYTANIRTISGVYNSESGSTEAVTKARGLVDDFEKAEGRRPRILVAKMG
                     QDGHDRGQKVISTAFADLGFTVDVGPLFQTPEETARQAVESDVHVVGVSSLAAGHLTL
                     VPALREELDKLGRDDMMIVVGGVIPPQDFDELYAKGADAIYPPGTIIPDSAIELLTKL
                     RERLDA"
     gene            2001..3125
                     /gene="meaB"
                     /locus_tag="PKJ61_10645"
     CDS             2001..3125
                     /gene="meaB"
                     /locus_tag="PKJ61_10645"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007433590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HOC14321.1"
                     /translation="MTQDRPPLWAESSPVPGRQYGDATPPAPKPSAVRRPRLDPQHLA
                     EQVLAGSRGGIARAITLVESSKPAHHAVAADLLRILRPYSGKAVRVGITGVPGAGKST
                     FIDALGRRLIDAGHRIAVLAVDPTSAKTGGSILGDRTRMGELAQSEHAFIRPSPAGSH
                     LGGVARTTRESMLVLEAAGYDVVLIETVGVGQSEVAVSGMVDTFLLIALARAGDQLQG
                     IKRGILEMADVITVNKADGDNESESRVTARELSIAMKLINSDPKAVRPPVLTSSSLTG
                     NGLDEVWQAIVDHRARLERSGQLEARRAKQQREWLWALVKGELEDALRTSPGVSAIRA
                     GLEAEVESGELSALEASAEILTAFAKDVPQLWSHRLDGIG"
     gene            complement(3129..3734)
                     /locus_tag="PKJ61_10650"
     CDS             complement(3129..3734)
                     /locus_tag="PKJ61_10650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14322.1"
                     /translation="MLSARSAVLIAAACAGLAGCFGGTSGGDAVPTSAVSSPAPAATA
                     GFTEHQVAGVRIGLPANWTETETSDGLGLQLADSGGNGTVMVIVNEVVRNSPGEIHYT
                     ASSYLEKALPWVLHFKDFRDIEITADLTGQARSLTLPGAENAAWLEVTGKRGNRPVAC
                     SVVAAQVGPNMAVIVGLQLPAQLWQQVMDTLRVDPNALEPT"
     gene            complement(3919..4785)
                     /locus_tag="PKJ61_10655"
     CDS             complement(3919..4785)
                     /locus_tag="PKJ61_10655"
                     /inference="COORDINATES: protein motif:HMM:TIGR04041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HOC14323.1"
                     /translation="MAELVEASGLITDFIEFSAVDGPGNRFVVFTQGCNLDCVACHNP
                     YTINPCIDCGDCVVSCPSGALSLDIAGKVLWDADTCTGGDTCIDVCEYDSTPKARTLA
                     VADVLTRLRPAAPFLSGVTVSGGEATQQAGFVRALFAAIKADPKLSRLTCFVDSNGDT
                     DSGDWDNLADVMDAAMIDLKCLDDVIHRRITGSSNARVLDSIRLLASRRQLYEVRLLL
                     LAGVNDADDLLAETGRWLASVDPRMRVKVIGYRHHGVRPSPIPLREPSAEQRAHYAEI
                     VRAQGDFEIVVV"
     gene            complement(4855..6402)
                     /locus_tag="PKJ61_10660"
     CDS             complement(4855..6402)
                     /locus_tag="PKJ61_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HOC14324.1"
                     /translation="MEMDTFKAEAERLVTDPAIGYDQKVRRLAGLAVSALPYPSLSPL
                     CAEALDARVICDMFEGNRPITPRYVLPDYAKALRNGVRYLELEPPTTLDDALSFLTIL
                     YTQVPSVTTYPVYLGDLDALLEPFVPADLPDVELDAKLRRFWIQLDRLLPDAFVHANV
                     GPSDSRVVRSIWRVERSLRQVVPNLTLKVTPDTPDDLIEDAVRTVFVTGKPHFVNHPM
                     MVADHGTDYAVVSCYNSLKTRGGAHSLVRLNLLEAVRRHVGGVDAFVTEALPHWVELT
                     AELGEARIRSLVEDQKFFETNFLATEGLIDLDRFSLMFGIFGLAECVNELMTREGQPE
                     ARYGHDDAANQVAWRLTRRIAELVAERPLPYCGGGGGFAYLHSQSGIDLDLDTTAGTR
                     LPIGDEPGMLEHISTVAPHHALFPAGISDIFHVEETVQNNPRAMVDVIRGALEVGMRD
                     FTFNLDSNEFIRITGYLVRKSDLIGIEEHGARHSSDYLAATAEVNHTVTTRAVKRVMA
                     AELSAGA"
     gene            6555..7622
                     /locus_tag="PKJ61_10665"
     CDS             6555..7622
                     /locus_tag="PKJ61_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007078487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOC14325.1"
                     /translation="MRQVTKIVIAATTAALTLTACAGPTTPAATSSEAAATSAPATSQ
                     APKLKVGMAFDVGGRGDQSFNDSAAAGLDKAAAEFGIEVKDAAATNGEAESARAERLQ
                     QLIDAGFTNIVAVGFAYANAVGQVAKENPDVKFAIVDDASEASTADNIMNITFAENEG
                     SFLVGAAAALKSESGHIGFVGGVETDLIKKFEAGFVAGAKAAKADIKVDSKYLTQPPD
                     FSGFASVDKGKAAAEGMYQNGADIVYHAAGGSGGGVFTAAKAAKKWAIGVDSDQALTA
                     AEDVRDVILTSMIKRVDVGVYTFIKSVHENAFKGGNTVFDLKADGVGYSTTNGHIDDI
                     KDKLEAYKADIIGGKITVPAK"
     gene            7781..>8103
                     /locus_tag="PKJ61_10670"
     CDS             7781..>8103
                     /locus_tag="PKJ61_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOC14326.1"
                     /translation="MSSAVEFQEPPPAPAGDGLAVELHGITKRFPGVVANRDIELSVR
                     KGTIHAIVGENGAGKSTLMKILYGVQLPDEGTIAINGEQVVLKSPTEAIRVGIGMVFQ
                     HFMLA"
BASE COUNT         1400 a         2649 c         2765 g         1289 t
ORIGIN      
        1 cgaatgcctt ctaccggcgc aacctcgcgg ccggccagaa gggtctttcg atcgccttcg
       61 acctggccac ccaccgcggc tacgactccg accacccgcg ggtgaccggt gacgtcggca
      121 tggccggcgt ggccgtggac tcgatcctgg acatgcggca gttgttcgac ggcatcccgc
      181 tcgaccagat gtcggtgtcg atgaccatga acggcgcggt gctgccgatc ctggcgctct
      241 atgtggttgc ggccgaggag cagggggtga agctggagca gctcgcgggc acgatccaga
      301 acgacatcct caaggagttc atggttcgca acacctacat ctacccgccg gcgccgtcca
      361 tgcggatcat cgccgacatc ttcgcgttca cttcggcgaa catgcccaag ttcaactcga
      421 tctcgatctc cggctaccac atgcaggagg ccggggcgac cgccgacatc gagatggcct
      481 acaccctcgc cgacggcgtg gagtacatcc gcgccggtga ggcggtgggc ctgaaggtcg
      541 accagttcgc gccgcggctg tcgttcttct gggcggtggg catgaacttc ttcatggagg
      601 tcgcgaagtt ccgcgcggcc cggctgctct gggcccggct ggtgcgccag ttcgagccga
      661 agaaccccaa gtcgatgagc ctgcggacgc attcgcagac ctcgggttgg tcgctgaccg
      721 cgcaggacgt gttcaacaac gtcggccgca cctgcctgga ggcgatggcc gcgacccagg
      781 ggcacaccca gtcgctgcac accaacgccc tggacgaagc gatcgcgttg ccgaccgact
      841 tctccgcccg gatcgcccgc aacacccagc tgttcatcca gcaggagtcg ggcacctgcc
      901 ggacggtcga cccgtgggcc ggttcggcct acgtcgagcg gctgacctac gagctggcca
      961 gcaaggcgtg ggatctgatc caggaggtcg aggcggccgg cggcatggcc aaggccatcg
     1021 aggccggcat cccgaagatg cgcatcgagg aggccgcggc ccgcacccag gcccggatcg
     1081 actccggccg gcagccgctg atcggcgtga acaagtacat cgtggagaac gatgtgctgc
     1141 cggaggtgct gaaggtcgac aacaacagcg tccgccagca gcagatcgac aagctgaccc
     1201 ggctgcgcgc cgagcgtgat tcggctgcga cgcaggctgc gctcgagaag ctcacctggg
     1261 ccgccgagaa tccggattcg accgacccgg agcggaacct gctcaagctg gccatcgacg
     1321 cggcgcgcgc caaggcctcg gtgggcgaga tctccgacgc gctggagaag gtgttcggac
     1381 gctacaccgc gaacatccgt acgatctctg gcgtgtacaa ctccgaatcg ggctcgaccg
     1441 aagccgtcac caaggcccgc ggcctggtgg acgacttcga gaaggccgag ggccggcgtc
     1501 cgcgcattct ggtggccaag atgggccagg acggtcacga ccgcggccag aaggtgatct
     1561 cgaccgcgtt cgcagacctc ggcttcaccg tcgatgtcgg cccgctgttc cagaccccgg
     1621 aggagaccgc acggcaggct gtggagtcgg acgtgcacgt ggtcggcgtc tcctcgctgg
     1681 ccgcgggtca cctgaccctg gtgccggcgc tgcgcgagga actggacaag ctgggccgtg
     1741 acgacatgat gatcgtggtc ggtggtgtga tcccgccgca ggacttcgac gagctctacg
     1801 ccaagggtgc ggacgcgatc tacccgcccg gcacgatcat cccggattcg gcgattgagc
     1861 tgctgaccaa gctccgggag cgcctggacg cctgacgaag ggccaagagg agatcccgga
     1921 tcaggcccgg gatgacgtgg aaactgtcat cccggcgcag gccgggatct ccttcgtctt
     1981 tgcgagttga aaggcaagtg atgacccagg atcgcccccc gctctgggcg gaatccagcc
     2041 ccgtccccgg acgccagtac ggggacgcga ccccgccggc acccaagccg tccgcggtac
     2101 ggcgtccgcg actggatccg cagcacctgg ctgagcaggt gctggcgggt tcgcgcggcg
     2161 ggatcgctcg ggcgatcacc ctggtcgagt ccagcaagcc ggcccaccac gcggtggcgg
     2221 ccgacctgct gcgcatcctg cgtccgtact cgggcaaggc ggtacgggtc ggcatcaccg
     2281 gcgtaccggg cgccggcaag tcgacgttca tcgacgccct cggccggcgg ctgatcgacg
     2341 ccggtcaccg gatcgccgtg ctagccgtcg acccgacgtc cgccaagacc gggggctcga
     2401 tcctcggcga ccgcacccgc atgggcgagt tggcgcagag cgagcacgcc ttcatccggc
     2461 cctcgccggc cggcagccat ctcggtggcg tggcccgaac cacccgcgaa tcgatgctgg
     2521 tgctggaagc cgccggctac gacgtggtgc tgatcgagac ggtcggggtt gggcagtcag
     2581 aggtggcggt ctccgggatg gtcgacacct tcctgctgat cgcgctggct cgcgcgggcg
     2641 accaactgca gggcatcaag cgcggcatcc tcgagatggc cgacgtgatc acggtcaaca
     2701 aggccgacgg cgacaacgag agtgaatcgc gggtcaccgc acgcgagctg tcgatcgcca
     2761 tgaagctgat caactccgac ccgaaggcgg tgcgcccgcc ggtgctgacc tcgagttcgc
     2821 tgaccggcaa cggcctggac gaggtttggc aggcgatcgt cgaccatcgg gcgcggctgg
     2881 aacgctccgg ccagttggag gctcgccggg ccaagcagca gcgggaatgg ctgtgggccc
     2941 tggtgaaggg cgagttggag gacgcgttac gcacctcgcc gggggtttcg gcgatccgcg
     3001 ccggcctgga ggccgaagtc gagagcggcg agctgtcggc gctggaggcg tccgcggaga
     3061 tcctgaccgc gttcgccaag gacgtgcccc agctctggtc ccaccgcctg gacgggatcg
     3121 gctgacctct aggtgggttc gagggcgttc ggatcgaccc gcagcgtgtc catgacctgt
     3181 tgccacagct gggcgggcaa ctgcaggccg acgatcaccg ccatgttcgg gccaacttgg
     3241 gccgctacca ctgagcaggc cacgggccgg tttccgcgct tgccggttac ctccagccag
     3301 gccgcattct ccgctcccgg gagcgtcagc gatcgggcct ggccggtcag gtccgcagtg
     3361 atctcgatgt ccctgaaatc cttgaagtgc agcacccagg ggagcgcctt ctccaggtat
     3421 gaggaagcgg tgtagtggat ctcaccggga ctgttgcgga ccacttcgtt cacgatcacc
     3481 atgacggtgc cattgccacc gctatcggct agctgaaggc cgagtccgtc gctcgtttcg
     3541 gtttcagtcc agtttgcggg gagcccgatt cttacgccgg caacctgatg ttcggtgaaa
     3601 ccggcggtgg cggccggtgc cggactggac acggcggacg tgggtaccgc gtcaccgccg
     3661 gaggtgccgc cgaaacagcc cgcgagaccg gcgcacgccg cggcgatcag caccgccgaa
     3721 cgcgcggaca acataggcag tttcctatcg ggatctaaat cgccagtcag tgtagccggg
     3781 ccggtgcggc tggcaccagt gtaaggggcg gaaggcctag aggcggcggc ctccttcgtg
     3841 acagctcgct cgttcctcgc gagcttcctc agggaccgtt acgcgggtgt ggtcgctcgc
     3901 tgccaacggt ggtcgaactc agaccaccac gatctcgaag tcgccttggg cgcggacgat
     3961 ctcggcgtag tgggcgcgct gttcggcgga cggttcgcgc agcggaatcg gggagggccg
     4021 gacgccgtgg tggcggtagc cgatcacctt cacccgcatc ctggggtcga cggacgcgag
     4081 ccagcgtccg gtttcggcca gcaggtcgtc ggcgtcattc accccggcca gaagcagcaa
     4141 gcgcacctcg tacagctgtc gccgtgaagc cagcagccga atcgaatcca gcactctggc
     4201 gttgctcgaa ccggtgatcc gacgatgaat gacgtcgtca aggcacttca gatcgatcat
     4261 cgccgcgtcc atcacgtcgg ccaggttgtc ccagtcgcct gagtcggtgt cgccattact
     4321 gtccacgaag caggtcagcc gggacagctt cgggtcggcc ttgatcgcag cgaacagggc
     4381 gcggacgaaa cctgcctgct gggtggcctc gccgccggag accgtcacac ccgaaaggaa
     4441 cggcgcggcc ggccgcagtc gagtcagcac gtccgcgacg gccagcgtcc gagcctttgg
     4501 ggtcgagtcg tactcgcaga cgtcgatgca ggtgtccccg ccggtacagg tgtccgcgtc
     4561 ccagagcacc ttcccggcga tgtccaagct caacgctccg gaagggcagg agaccacgca
     4621 gtcgccgcag tcgatgcatg ggttgatcgt gtacgggttg tggcaggcca cgcagtctag
     4681 gttgcagccc tgggtgaaca ccacgaaccg gttgcccgga ccgtccacag ccgagaactc
     4741 gatgaagtcg gtgatcaggc ccgaggcttc gacaagctca gccatcgtta gctccggacg
     4801 atccctgagc ttgacgaagg gtcaacgggg cttcggcaag ctcagccacc gttcctaagc
     4861 acctgcgctc agctcggcgg ccattacccg cttcacggct cgggtggtga ccgtgtggtt
     4921 cacctcggcg gtggcggcga ggtagtccga cgagtgccgg gcgccgtgct cttcgatgcc
     4981 gatgaggtcg gacttgcgca ccaagtagcc ggtgatccgg atgaactcgt tggaatccag
     5041 gttgaacgtg aagtcgcgca tgcccacttc gagcgcgccg cgaatcacgt ccaccatcgc
     5101 ccgcgggttg ttctgcacgg tctcctcgac gtggaagatg tccgagatgc cggcggggaa
     5161 cagcgcgtgg tgcggcgcga cggtcgagat gtgctcgagc atcccgggct cgtcgccgat
     5221 tggcaggcgg gtgccggcgg tggtgtccag gtcgaggtcg atgccgctct gcgagtgcag
     5281 gtaggcgaag ccgccaccgc cgccgcagta ggggagcgga cgctccgcca ccagttcggc
     5341 gatgcgcctg gtgagccgcc acgcgacctg gttggcggcg tcgtcgtggc catagcgtgc
     5401 ctccggctgg ccctcgcggg tcatcaactc gttcacgcac tcggccagcc cgaagatgcc
     5461 gaacatcagc gagaaccggt cgaggtcgat caggccctcg gtggccagga agttcgtctc
     5521 gaagaacttc tggtcctcca ccagggagcg gatccgggcc tcgccgagtt ccgcggtgag
     5581 ctccacccag tgggggagcg cctcggtgac gaacgcgtcc acgccgccga cgtgccggcg
     5641 caccgcttcg agtaggttca gccgcaccag cgagtgggcg ccgccgcggg tcttcagcga
     5701 gttgtagcag ctcaccacgg cgtagtcggt gccatggtcg gcgaccatca tcggatggtt
     5761 gacgaagtgc ggcttgccgg tgacgaagac cgtgcgtacc gcgtcctcga tcaggtcgtc
     5821 gggagtatcg ggagtcacct tcagggtcag gttcggtacg acctggcgca gcgagcgttc
     5881 cacccgccag atcgagcgca ccacgcggga atcggacggg ccgacgttgg cgtgcacgaa
     5941 cgcgtccggg agcaaccggt cgagctgaat ccagaagcgg cgcagcttgg cgtccagttc
     6001 gacgtccggc aggtcggccg ggacgaacgg ctccagcagc gcgtccaggt cgccgaggta
     6061 gaccgggtag gtggtcaccg aaggcacctg ggtgtacagg atcgtcagga aggacagcgc
     6121 gtcgtcgagg gtggtcggcg gctccagttc gaggtagcgg acgccgttgc gcagggcctt
     6181 ggcgtagtcc ggcagcacgt agcgcggcgt gatcggccgg ttgccctcga acatgtcgca
     6241 gatcacccgg gcgtccagtg cctcggcgca cagcggcgaa agcgacgggt agggcagggc
     6301 gctcaccgcc agcccggcca ggcgccgcac cttttggtcg tacccgatcg ccgggtcggt
     6361 cacgagtcgt tcagcctcgg ccttgaaggt atccatctcc atttccgaag tctatcgccg
     6421 accaggagaa tttacgtttc gataacggtt ggtcgcagct tcgtcggagg cggtcgagcg
     6481 cgacgccgtg gaggactaga gtctgaatct cggcgtggca tccacgcttg aatgatccat
     6541 ataaggagac acccgtgcga caggtgacca agatcgttat cgcggcgaca accgccgccc
     6601 tgaccctcac cgcttgtgct ggcccgacca cccctgcggc cacgagcagc gaagccgcag
     6661 cgaccagcgc tccggcgacg tcgcaggccc ccaagctcaa ggtcggtatg gccttcgacg
     6721 tgggcggtcg cggcgaccag tccttcaacg actccgctgc cgccggcctg gacaaggccg
     6781 ccgccgagtt cggcatcgag gtgaaagatg ccgccgcaac caacggtgag gcggagtcgg
     6841 cccgcgccga gcgtctgcag cagctgatcg acgccggctt caccaacatc gttgccgtgg
     6901 gcttcgccta cgccaacgcg gtcggccagg tcgccaagga gaacccggac gtcaagttcg
     6961 ccatcgtcga cgacgcgtcc gaggcttcca ccgccgacaa catcatgaac atcaccttcg
     7021 ccgagaacga gggctccttc ctggtcggcg cggccgccgc gctcaagtcc gagtcgggcc
     7081 acatcggctt cgtcggtggc gtcgagaccg atctgatcaa gaagttcgag gccggcttcg
     7141 tcgccggtgc gaaggcagcc aaggctgaca tcaaggtcga ctccaagtac ctcacccagc
     7201 cgccggactt ctcgggcttc gcgtccgtcg acaagggcaa ggctgccgct gagggcatgt
     7261 accagaacgg tgcggacatc gtctaccacg ctgccggcgg ttccggtggc ggcgtgttca
     7321 ccgctgccaa ggcggcgaag aagtgggcga tcggcgtcga ctccgatcag gcgctgaccg
     7381 ctgccgagga cgtccgcgac gtcatcctca cctcgatgat caagcgcgtc gatgtgggcg
     7441 tctacacctt catcaagtcg gtgcacgaga acgcgttcaa gggcggcaac accgtcttcg
     7501 acctgaaggc tgacggcgtg ggctactcca ccaccaacgg tcacatcgac gacatcaagg
     7561 acaagctgga ggcctacaag gctgacatca tcggcggcaa gatcaccgtt ccggctaagt
     7621 gatttgaggc gacgaccgag cggtagttga cccgtaattc cccggcggcc cgggcgcacc
     7681 gtcaaggtgc gtccgggccg ccgactttcc cactagagtg cgatcgaggc cgtgggcact
     7741 gatgcctggc gggccagcaa actgacgagg agaaccgact atgtcaagtg ctgtcgagtt
     7801 ccaagagccg ccaccagcgc cggccggcga cggactcgcg gtggagctgc atgggatcac
     7861 caagcggttc cccggagtgg tcgccaaccg cgacatcgag ctgagcgtcc gcaagggcac
     7921 cattcacgcc atcgtgggcg agaacggcgc cggcaagtcg acgctgatga agatcctcta
     7981 cggagtccag ctgcccgatg agggcacgat cgcgatcaac ggcgagcagg tggtactgaa
     8041 gagcccgacc gaggcgatcc gggtgggcat cggcatggtg ttccagcact tcatgctggc
     8101 cga
//
LOCUS       DAOPRQ010000094         7890 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_7652_length_7890_cov_11.891895, whole genome shotgun sequence.
ACCESSION   DAOPRQ010000094 DAOPRQ010000000
VERSION     DAOPRQ010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 7890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7890
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7652_length_7890_cov_11.891895"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(30..1328)
                     /locus_tag="PKJ61_10675"
     CDS             complement(30..1328)
                     /locus_tag="PKJ61_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013160698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HOC14327.1"
                     /translation="MPAELTTPATPASLPRGDRVRLWTATRVGDAVALASRLAGKGSG
                     ASIRGQILTRLAPDAFAQLLQGRRVLAVSGTNGKTTTTHLLAAAVRAGLGADADRLVT
                     NADGANLHYGIASALSQRRRADIAVLETDERVVSDMIAKARPELLVLLNFSRDQLDRH
                     HEIKALGRAWRVALAAAGKGGPTIVANASDPLVVWSAGTAARVIWVETVTAWSADGAL
                     CPGCGAVLTHTETAAGPRWRCPGCELHQPEPQYRVSGDQIVLPSGKMVRPDLQVPGSF
                     NVSNAACALAAAIEFGVPLPQALAGMHTVTAPAGRFATATFGATTARLLLAKNPAGWV
                     EALPLAASDPVILAIDSVAADGKDVSWLWDVPYEQLAGRTVVCTGPRAQDLAVRLSYA
                     GVDHEVIPDLRAALGERPRPVDVIATYTPFQRLLKMAGLR"
     gene            complement(1361..2146)
                     /locus_tag="PKJ61_10680"
     CDS             complement(1361..2146)
                     /locus_tag="PKJ61_10680"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOC14328.1"
                     /translation="MAEPIPWRSFVALGDSNTEGLQDFDESGRPAGWADRFVQHLADR
                     AGTGINYANLAIRGRLLRPILDEQVEPALALKPDLVSLWGGGNDLLRFGADPDEMSAA
                     METAVVRFREAGADVLIGLGVDAKDSPIIGLTRNRAAIFNLNLTSIARRHGAHIIDGW
                     SLASLRDWRMWDDDRLHLNGAGHERMAQAALAALGLAPDDPDWATPLPPAPVLSRRER
                     FDWNYVWARDHLLPWLGRRVRRTSSGAGRVPKQPDYRLVEPRN"
     gene            2395..3051
                     /locus_tag="PKJ61_10685"
     CDS             2395..3051
                     /locus_tag="PKJ61_10685"
                     /inference="COORDINATES: protein motif:HMM:NF024108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HOC14329.1"
                     /translation="MVRLPGSDDRRRPHNPRQHLARRLPRPLGACRPTRARRGVTWPA
                     RAVRPRNPPHRAGHPPPARHRGRCGCLRVVGCGVRRRPHRDSAQARRTLARSQLDPPL
                     LLALDEIGNLAPLPSLPTLMAEGGGTGITTMPVLQSPAQARDRWSEHQAGAIWDASIV
                     KIILGGASTSACATTSGARHAPSRSTKSVRGWSGGRSRCSPPATGPRANFIRSWWRLG
                     "
     gene            2988..3383
                     /locus_tag="PKJ61_10690"
                     /pseudo
     CDS             2988..3383
                     /locus_tag="PKJ61_10690"
                     /inference="COORDINATES: protein motif:HMM:NF019149.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            3754..4002
                     /locus_tag="PKJ61_10695"
     CDS             3754..4002
                     /locus_tag="PKJ61_10695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14330.1"
                     /translation="MLSNAADIYEHADDANRRLLNQALFKTIYIDEDNDVRVGYRNPY
                     DGLSIPGLHADALSWAAEAKWARLTPQPRADPWSRVQT"
     gene            4175..5341
                     /locus_tag="PKJ61_10700"
     CDS             4175..5341
                     /locus_tag="PKJ61_10700"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HOC14331.1"
                     /translation="MLHEQRERRGFGRLRQLPSKRWQAAYTGPDTQLHSPPSTFMSKD
                     DAVAWLAAERKLIDLDVWTPPSERRAVRRAVGQSFRDFADEWLASRKTAKGEPLKART
                     VADYRRYLDGHILPTLGSLPLRQLTPTRVQEWYDDLDAETPTQRAHVYQLLRAITTTA
                     TDKKLIPTNPCKIPGAGRAQRARKITPATPAELAAIAANMPKRLQLAVLLGGWCALRY
                     GEIAELRRRDIDLKRGTITVARGVTWPSVRDPETGKLVSKAVVGTPKSEAGLREVHIP
                     PHILPAVEAHLAEHTDRGRDAMLFASSTGNHIHPRAFGWQYHQARVKAGRPDLRFHDL
                     RHTGAVLAAQTGATLAELMGRLGHSTSSAAMRYQHAAADRDKAIAEALSRLAEA"
     gene            5760..5912
                     /locus_tag="PKJ61_10705"
     CDS             5760..5912
                     /locus_tag="PKJ61_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14332.1"
                     /translation="MLRVLFGEREPLGSLPFDLPGSMDAVIASRSDVPFDTAEPTFRF
                     GHALRY"
     gene            complement(6635..7063)
                     /locus_tag="PKJ61_10710"
     CDS             complement(6635..7063)
                     /locus_tag="PKJ61_10710"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HOC14333.1"
                     /translation="MRVALDLALAPGVVERLAGGNEPELTSLCDEMTSAAERNELFAA
                     ADRAFHLSLAARLGNEIYGQLVAAFWDVHTIVAPRLGVPSTRDAVDTALAHRAMLSAA
                     LSGNLDAYREAVSAHYRPLLRSLGSAQQHGLTTTLEPVPS"
     gene            7096..7422
                     /locus_tag="PKJ61_10715"
                     /pseudo
     CDS             7096..7422
                     /locus_tag="PKJ61_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002520859.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
     gene            7521..>7890
                     /locus_tag="PKJ61_10720"
     CDS             7521..>7890
                     /locus_tag="PKJ61_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014598960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOC14334.1"
                     /translation="MARNLTLILLVAVGVYFLMPIMWMIIASSKSNRDLLATFGFWFA
                     ELNWDANWTSLMSWTQNLFPRWVLNSMFYSTVAAAVGTLISVACGHAIAKFAFPGRRA
                     LLVVIMTGLLMPIALLTVPLY"
BASE COUNT         1263 a         2687 c         2710 g         1230 t
ORIGIN      
        1 cgactggtag accagcacga tctcgacgct catcgcagcc ccgccatctt cagcagccgc
       61 tggaacggag tgtaggtcgc gatcacatcc accggtctgg ggcgttcacc cagcgcggcc
      121 cgcagatcgg ggatcacctc atggtcgacg ccggcgtagc tgagccggac ggcgaggtcc
      181 tgggcgcgcg ggccggtgca gaccaccgtc cggccagcca gttgttcgta gggcacatcc
      241 cagagccagg agacgtcctt gccgtcggcg gccaccgaat cgatcgccag gatcaccgga
      301 tcggacgcgg ccagcggcag cgcctcgacc cagccagcgg ggttcttggc cagcagcagg
      361 cgggccgtgg tcgcgccgaa ggtcgcggta gcgaagcgtc cggcaggggc ggtgacggtg
      421 tgcatcccgg cgagcgcctg cggcagcggg acgccgaact cgatcgcggc ggccagggca
      481 caagcggcgt tcgagacgtt gaaggagccg ggaacctgca gatcggggcg aaccatcttg
      541 ccgctgggca gcacgatctg atcaccgctc acccggtact gcggctcggg ctggtgcagc
      601 tcgcagcccg gacagcgcca gcgcgggccg gctgcggtct cggtgtgggt cagaacggcg
      661 ccgcatccgg ggcacaacgc cccgtccgcg ctccaggcgg tgaccgtctc cacccagatc
      721 acccgcgccg ccgtgcctgc cgaccagacg acgagcgggt cggacgcgtt cgcgacgatg
      781 gtgggaccgc ccttcccggc agcggccagg gctacccgcc aggcgcggcc gagcgctttg
      841 atctcgtggt gccggtcgag ctggtcgcga ctgaagttga gcagtaccag cagttcggga
      901 cgggccttgg cgatcatgtc cgagaccacg cgttcgtcgg tctccagcac cgcgatatcg
      961 gccctacgtc gctggctgag cgcggacgcg atgccgtagt gcaggttggc gccgtcggcg
     1021 ttggtgacca gccggtctgc gtccgcgccc aggccagcac ggacggccgc cgcgagcagg
     1081 tgcgtggtgg tggtcttgcc gttggtgccg gagacggcga gcacccggcg tccctgaaga
     1141 agttgggcaa aggcgtccgg ggcgagccgg gtgagaatct gtccgcggat cgaggccccc
     1201 gagcccttac cggcgagccg ggacgcgagc gctactgcgt cgccgacgcg ggtggccgtc
     1261 cacagccgga cgcggtcgcc ccgcggcagg gacgctggcg tggcgggcgt ggtcagctcg
     1321 gcaggcatga cccccattgt gccgtgcggc actgaccacg tcagtttcgc ggttccacca
     1381 ggcgataatc gggctgcttc gggacgcgtc cggcgccaga gctcgtgcgg cgtacccggc
     1441 ggccaagcca gggcagcagg tggtcgcgcg cccagacata gttccaatcg aagcgttcgc
     1501 gacggctcag gacgggtgcc ggcggtagcg gcgtcgccca atcgggatcg tccggggcga
     1561 ggccgagcgc ggcgagggcg gcctgcgcca tccgctcgtg gcccgctccg ttcaggtgca
     1621 ggcgatcgtc gtcccacatc cgccagtcgc gcaggctggc caggctccag ccgtcgatga
     1681 tgtgcgcgcc gtgccggcgg gcgatgctgg tgaggttcag gttgaagatc gccgcgcggt
     1741 tgcgggtgag gccgatgatg ggggagtcct tggcgtccac gcccagcccg atcagcacgt
     1801 ccgcaccggc ttcgcggaag cggacgaccg cggtctccat ggccgcggac atctcgtccg
     1861 ggtcggcgcc gaagcgcagc aggtcgttgc cgccgcccca cagcgagacc aggtcgggct
     1921 tcagggccag cgcgggctcc acctgctcgt cgaggatcgg acgcagaagg cgtccgcgga
     1981 tggcaaggtt ggcgtagttg atgccggtgc cggcgcgatc ggcgagatgt tgtacgaagc
     2041 gatcggccca gccggccggc cgtccggatt cgtcgaagtc ctgcagacct tcagtgttgg
     2101 agtcgccgag tgcgacgaat gagcgccagg ggatgggttc ggccacggtg cgtccttcaa
     2161 ttcgaggaga tttctgagac tagctcgccg ggccttgctc ggacgcaccg tcttgaggca
     2221 ccaaggtcgt tcggcatcct tggatcgctc aggcgcactg cggacggccg cccgcctggt
     2281 agctgagggc gacgatgccg gtgctggggc cgagctgttc cgctggacac tcgacccctc
     2341 tgctgcctcc gaggcggtcg cgatcctgaa ctcccacccg gacgcggcga tggggtggta
     2401 cgactccctg gcagcgatga tcgacgccga ccccacaacc cgcgacagca tctggcaagg
     2461 cgtctccctc gccctctcgg cgcttgccga cccacgcgtg ctcgacgcgg tgtcacctgg
     2521 cccgcacgag cagttcgacc ccgaaaccct cctcaccgag cagggcaccc tccacctgct
     2581 cgccaccggg gcaggtgcgg gtgcctccgc gtcgttggtt gcggcgttcg tcgaagacct
     2641 catcgagaca gcgcgcaggc tcgccgcacg ctcgcccgga gccagcttga tccgccgctg
     2701 ctgctcgcgc tcgatgagat tgggaacctc gcgcccttgc cgtcgctccc gacgctcatg
     2761 gctgaaggcg gcggcaccgg catcacgacc atgcccgtgt tgcagtcgcc cgctcaagcc
     2821 cgcgaccggt ggagcgagca tcaggccggc gcgatctggg acgccagcat cgtgaagatc
     2881 atcctcggcg gcgcatccac gtcggcgtgc gcgacgactt cgggcgcgag gcacgcaccg
     2941 tcgagatcga cgaagagcgt gcgcggctgg tcaggtgggc gttccaggtg ttcgcctccg
     3001 gcgactggac cacgagccaa cttcatcagg agttggtggc gcttgggctg acgacagccg
     3061 cgacgccgcg gcgaccttct cggcccatcg ccaagtcatc ggtgcaccgg atgctgacga
     3121 acccgtacta caagggaagc gtccggttcc aaggcgtcac ctacgccgga gcacacgagg
     3181 cgatcgtccc caacgaggtg tgggaccagg tacagaccgt gctcggtaca caccgatccg
     3241 ccgctgacgc aacccaggta cgcgagcact acttgaaggg cacggtgttc tgcgggcagt
     3301 gcggctcgcg actgatcgtg tgcaacgcga agagcaccca aggcacgatc tacccctact
     3361 tcgtgtgcgc gagccggcac ggagccagag gcgactgcac ccgacaggcg atgctgatcg
     3421 agcaggttga acgactcatc gaacgcttct gcgccaaggt gcagatcgac ccggaaacga
     3481 tcgaggcggt ctcggcgagg atccatgccc ggttcgacga gatgctggcc gaaggagccg
     3541 ccgaactcgc cgacctcgcg tatcggagga ccgagctcgg agtcgagcaa cacaagctgc
     3601 tccaggctca ctacgcaggg gcgatcccgc tcgacctgct caagcgagaa caggaccgga
     3661 tcacccgcgt cgctggagac catcgagtac cggatcaccg cgcaccacgg ccactgcgcc
     3721 gacgcgagag agaacctcga cgactcactg aagttgctgt ccaatgccgc cgacatctac
     3781 gagcacgccg acgatgcgaa tcgtcggcta ctcaaccagg cgctgttcaa gacgatctac
     3841 atcgacgagg acaacgacgt gcgcgtcggc taccgcaacc cgtacgacgg gctgagcatc
     3901 cccggcctcc atgccgacgc gctgagctgg gccgccgagg caaaatgggc caggctgacc
     3961 ccgcaaccaa gggcggaccc ttggtcgcga gttcaaacct gacccgtttg gggtgagtaa
     4021 cgggactcga tccagatact cgcagaagtg acgagtaacg ggataccccc ttgtaagagc
     4081 cgtgctgact aggccgtacg cctcaccata cgcgccccgt tacttgggca gaagtgggca
     4141 gacgtgggcg ctgatgggct gctatattgc acgtatgttg cacgaacagc gggagcggcg
     4201 aggcttcgga cgcctgcggc agctgccgtc gaagcgctgg caggcggcct acaccggccc
     4261 ggacacccag cttcacagcc cgccctcgac gttcatgagc aaggacgacg ccgtggcctg
     4321 gctggccgcc gaacgcaagc tgatcgacct cgacgtgtgg acgccgccga gcgagcggcg
     4381 agcggtccgc agggccgtgg ggcagagttt cagggacttc gccgacgagt ggctggccag
     4441 ccgcaagacg gccaagggcg agccgctcaa agcgcggacg gtcgccgatt accggcgcta
     4501 cctcgacgga cacatcctcc cgacgctggg gagcctgccg ctgcggcagc tcaccccgac
     4561 ccgggttcag gagtggtacg acgaccttga cgccgagacg cccacccagc gcgcgcacgt
     4621 ctaccagctg ctgcgggcga tcaccacgac cgccaccgat aagaagctga ttccgaccaa
     4681 cccgtgcaag atcccgggcg ccgggcgcgc ccagcgggcc aggaagatca cgccggcgac
     4741 ccctgccgag ctggctgcga tcgccgcgaa catgcccaag cggctccagc tggccgtcct
     4801 gctgggcggc tggtgcgcac ttcggtacgg cgagatcgcc gaactccgac gtcgagacat
     4861 cgacctgaag cgggggacca tcaccgtggc ccgcggcgtg acctggccca gcgtccggga
     4921 tccggagacc ggcaagctgg tctccaaggc cgtcgtgggg actcccaaga gtgaggccgg
     4981 ccttcgcgag gtgcatattc cgccgcacat cctcccggct gtggaggcac atctggccga
     5041 gcacaccgac cgcggccggg acgcgatgct gttcgcctcg tccaccggaa accacatcca
     5101 cccgcgcgcg ttcggctggc agtaccacca ggcaagggtg aaggccggac ggcccgacct
     5161 gcgattccac gacctgcgcc acaccggcgc cgtcctggct gcccagaccg gcgccaccct
     5221 cgccgaactc atgggcaggc tcggccactc gacatccagc gccgccatgc gctaccagca
     5281 cgcggcagcc gaccgcgaca aggcgatcgc tgaggcgctg tcgagactgg cggaagcctg
     5341 accgactctt catccggcat agaaggccgg ctcgtccctt caaccgcttc caagcgcttc
     5401 gccgtcccac tcggctaggg accaggttct tcccctgagc cgcggcgtcc gtgtctacgc
     5461 cgaaggcgtg gccgccgatg ctcttggcgc gtacgcgagc gtggtggacg cgcctgagca
     5521 ggccgacgtc gcgatcctgc ggctgaaagc gccctacgag ccgcgaaaca acgggttcga
     5581 ggcgttcttc cacgcgggat ccctcgaatt cacggcggag cagcttgagc ggatcgggac
     5641 gatcgtcgcc gccgcgccaa cagtgatcga ccctacctgg agcgacccgc cgtgctcggt
     5701 gggcttgata agtccgcggc cgccgtcatc gccaactacg gcgcgcctgc gatgcggcgg
     5761 tgctccgggt gctattcggc gagcgcgagc cgctcggctc gctgcccttc gatctgcccg
     5821 gatccatgga cgcggtgatc gccagccgca gcgacgttcc gttcgacacg gccgagccca
     5881 ccttccggtt cggccacgcc ctgcgctact gatttcttgt caagggggta tggcgtggtg
     5941 gcgtcctccg tggtcggggg gacgccgccg ctgttgttca cggggctggt ggcctgtcgg
     6001 cgtggtcgtc gacgcggtgg tcagactgat atgcgggttg gataccgcct gacgggttgt
     6061 tcggctgggg cgttgccgct ggtctgaggt cgaaaccccg gttgtggggt tcgcagtaga
     6121 agcgcgggtc actccgatac gcgtgccctt ccggcgttcg cgaacgtttg tgttcccggc
     6181 tacatggtgg ccagggccca gcaccagccg gccagctcgc gggcgatcgc gacgttggcg
     6241 gccgtttgtg gcgggcgtcg aagtggagcc aaacgctggt ggaggcgttt gttgcctgcg
     6301 tgctgcggtg aagatgacgt cttcgaagca ggtgtcgaac gcggcctggg tgccgtattg
     6361 gtcgaaccgg atgccacgca gccaccgctg gtggtcaggg gtccaggcgg gtccgccgtt
     6421 gtacacgtgc ccgtgccgga tcagcagctt cccgagccga ggcactcatc ggacggctcg
     6481 gtctgtcggt gagcggcgtg ccttgcgctg cacgagcttc cgatggccgc tccggcgctc
     6541 ctccgagacc ggccagtggg gcctgccgaa cctgggcacg gcgaccacag ccgtgacgac
     6601 cggtccgatc cgcccggttg ccgctcctgc ctcctcagct agggacgggt tcgagcgtcg
     6661 tggtgagacc gtgctgctgg gcggatccga gggagcgcag gagcggccgg tagtgcgctg
     6721 acacggcctc tcgataggcg tccaggttcc cgctcagcgc ggcgctcagc atggctcggt
     6781 gcgccagcgc ggtgtcgacc gcgtcacgcg tactggggac gccgaggcgt ggggccacga
     6841 tcgtatgcac gtcccagaag gcggcgacga gctggccgta gatttcgttg ccgagccgcg
     6901 cagccagcga caggtggaag gcccgatcgg cggcggcgaa caactcgttc cgctcggcgg
     6961 cagacgtcat ctcgtcgcac agtgaggtga gttcgggttc gttgcctccg gcgagacgtt
     7021 cgaccactcc tggcgcgagg gcgaggtcga gggcaacgcg cacttcgacg acctgccgca
     7081 ggacctcgaa gtcgtctcta cgaggcggcc cggatcgacg gcgccaacgg ctggcagatc
     7141 accacgaaga tcaagatccc gttgattcga ggcgccgcgt tgctggcggt cctgctgtcg
     7201 atcatcggca cgatccagct gttcaacgaa ccggcggtga tgcgcaccat tgcgtcctgg
     7261 atgggccacg actaaacccc gatgatgatg gcctacaaca ccatgatggg agcgatcact
     7321 ccgagcggcg ccgggccggc gtcggccatt tcgatcctga tggcgctggt ggccggtgtc
     7381 ctcgccgtcc tctacgccct cgtccagcga aaggcggact gagcaccgtg gccaagccaa
     7441 ctctcgccac cactccccgc ggtcaggagt tccgtccctc ggtgggaggg ctgccgcccc
     7501 gggcctacgc accgtccacc atggcccgga acctcacgct gatcctgctc gtcgcggtcg
     7561 gggtctactt cctgatgccg atcatgtgga tgatcatcgc ctcatccaag tcgaaccggg
     7621 acctgctcgc caccttcggg ttctggttcg ccgagttgaa ctgggacgcc aactggacgt
     7681 ccctgatgag ctggacccag aacctcttcc cgcgctgggt actgaactcg atgttctact
     7741 ccacggtggc ggccgccgtc ggcaccctga tctcggtggc ctgcggccac gcgatcgcca
     7801 agttcgcctt cccgggccgt cgtgcgctgc tggtggtcat catgaccggc ctgcttatgc
     7861 cgatcgcgct gctgaccgta ccgctgtacc
//
LOCUS       DAOPRQ010000095         7835 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_7722_length_7835_cov_14.308612, whole genome shotgun sequence.
ACCESSION   DAOPRQ010000095 DAOPRQ010000000
VERSION     DAOPRQ010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 7835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7835
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7722_length_7835_cov_14.308612"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            144..347
                     /locus_tag="PKJ61_10725"
     CDS             144..347
                     /locus_tag="PKJ61_10725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14335.1"
                     /translation="MTYQLIQSRHSAPSLPRSLSAALAALRSLLAKVAHRLTLPTQVF
                     DDPDFGRFRGWPESEVQLRRWPY"
     gene            395..685
                     /locus_tag="PKJ61_10730"
     CDS             395..685
                     /locus_tag="PKJ61_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HOC14336.1"
                     /translation="MELEFTADVIEWRGPAPFVYAVVPEAEGEDIAAVARALTYGWGM
                     VLVTATIGATRFTTALYPKDGGYRLPIRVVVQRAESIRVGGRVQVRLELGTP"
     gene            737..1393
                     /locus_tag="PKJ61_10735"
     CDS             737..1393
                     /locus_tag="PKJ61_10735"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOC14337.1"
                     /translation="MQRLLSALDRMNRAHPWSHNDAYAGLVLHQARKTQRGGGSSALD
                     VGCGTGRLLRRLAECFPQAVGIEADAVTAGLAAAAVATFPAATVVNARFPVADGRRYD
                     FVSMVAVLHHLPLADGIAAVREVVAPGGRLVIVGVYREARADALFSVISLLLNPLIGM
                     ILHPRPADRTLEQLRAPAMPASEPYREIRSALRAALPGVRVRRGFFWRYVATWQAPRT
                     "
     gene            1549..1746
                     /locus_tag="PKJ61_10740"
     CDS             1549..1746
                     /locus_tag="PKJ61_10740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14338.1"
                     /translation="MVGAVHTAFEYGRMVATAEYNSAPSSVALFFMVPHLAVSMLLLV
                     AAVFTPRRKRANEPADTRSPR"
     gene            complement(1864..2865)
                     /locus_tag="PKJ61_10745"
     CDS             complement(1864..2865)
                     /locus_tag="PKJ61_10745"
                     /inference="COORDINATES: protein motif:HMM:NF019468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HOC14339.1"
                     /translation="MTSRPAKVPNRVRTTVLALVVPLVLLAAALAWAVAMLPELPDQV
                     ATHWGADGLPDGFGSPVSTLVMLAAIVVPFTIGMWAIGFYLGSTASTRRLSVFFSIWF
                     AVMISGLLVGSLAVQRGLTDATLAPGIGVPMAVSFAVATLAGALAALAIPADRPQPTT
                     APVPDGAERLTLGANEQAAWVRTAELPGLGWIMTAGAILTVLLGGIALLGGVGVGVTT
                     LVILAMVTLLLVLLGRWTVTVDHDGLAARSVLPRPRTVVPLNEVEAAEVVDVQPLADF
                     GGWGYRIDLTGRVGIVLRKGEAILVHRTGGRQLVITVDDAATGVALLNTLADRARLR"
     gene            complement(2862..3215)
                     /locus_tag="PKJ61_10750"
     CDS             complement(2862..3215)
                     /locus_tag="PKJ61_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015069419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOC14340.1"
                     /translation="MLFRIDPSATEPLFAQLAAQLRAGVADGSLRSGERLPAARELAA
                     SLNVNLHTVLRAYQELRDEGLIELRRGRGAVVTERATQDLAPLRRALAAVATESRRLE
                     LSAETTIALLKETLR"
     gene            complement(3379..4281)
                     /locus_tag="PKJ61_10755"
     CDS             complement(3379..4281)
                     /locus_tag="PKJ61_10755"
                     /inference="COORDINATES: protein motif:HMM:NF016152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HOC14341.1"
                     /translation="MEHPLMFDEADPWYQRVRGLALALPGAQEKVSHGRPTFYTKKVF
                     AYYGGSLKVDGAWVRHSQSVVLVGDLETISILRAQDCAYNPDYLGPSGWTGLDLDEHT
                     DLAELADWIEASYRFTAPRTLVAKVPAGRAFVDMLTGGNPRTLLGVTEVLAELRADPS
                     RISELIDCCTAADAVVRMRAADALEKFARERPELLTPFVDRLDAELAASTQPSIQWHL
                     AQLWGEVPLTAAQRARAAEWLIRTLDDADDWIVLTCAMSALATLAAADTSLRPALRER
                     LQRHSKSRLPAVAKRAVRLLAALD"
     gene            complement(4312..4668)
                     /locus_tag="PKJ61_10760"
     CDS             complement(4312..4668)
                     /locus_tag="PKJ61_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008356074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOC14342.1"
                     /translation="MAIARFPSVVLDCPDPAVLAEFYGRLLDWKVQLDGDWAEVRADY
                     GQAICFQQVADYRPPVWPGQEVPQQSHLDFVVDDLDAAAAATLELGAHKAEHQPGETF
                     RVFLDPAGHPFCLCVS"
     gene            complement(4747..5127)
                     /locus_tag="PKJ61_10765"
     CDS             complement(4747..5127)
                     /locus_tag="PKJ61_10765"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HOC14343.1"
                     /translation="MILVDTSIWIDHLHQGEPRLVALLNRNEVLSHPLVTGELALGSL
                     ADRDTVLGLLAHLPHAASASDAEILAFIEAQALYSRGLSLVDVHLLAATRLTPAARLW
                     TRDRRLLTAAEELDLAVAENTLIP"
     gene            complement(5124..5336)
                     /locus_tag="PKJ61_10770"
     CDS             complement(5124..5336)
                     /locus_tag="PKJ61_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009213360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HOC14344.1"
                     /translation="MQEVTMRTTVTIDSDLLALAAELTGTSERSTLFRKGLEALIQQE
                     SARRLARLGGTDPAAVSAPRHRGSET"
     gene            complement(5378..6736)
                     /gene="dnaB"
                     /locus_tag="PKJ61_10775"
     CDS             complement(5378..6736)
                     /gene="dnaB"
                     /locus_tag="PKJ61_10775"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOC14345.1"
                     /translation="MSLAEVIDYEEASYAGPDRTPPQDVAAEQSVLGAMLLSKDAIGD
                     VAEIVKGRDFYRPAHETIYDAILNLYGRGEPADAITVADELAKLGELGRIGGHIYLHD
                     LLSMVSIASNAAYYAEIVREKAILRRLVEASIKISQLGYAGQGDVAGIVDAAQQAIYE
                     VAEGKAGDDYEPLSALMESTLDEIEALSSHGIMAGVPTGFVDLDELTNGLHAGQMVIV
                     AARPGVGKSTLGLDLARAASIQHGLCSAFFSLEMTKTEIVMRLISAEAQVPLQDIRKG
                     HMSDENWARIARKTGDVSSAPLFIDDSPNLTMMEIRAKARRLKQRHDLKLVIVDYMQL
                     MSSGKRVESRQLEVSEFSRQMKLLAKELEVPVVALSQLNRGPEQRTDKKPMLSDLRES
                     GSLEQDADVVILLHRDDMYNNSSERTGEADFIVAKHRNGQTKTVTVAFQGHYSRFVDM
                     QH"
     gene            7215..7553
                     /locus_tag="PKJ61_10780"
     CDS             7215..7553
                     /locus_tag="PKJ61_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020577217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HOC14346.1"
                     /translation="MTANHEEQLSHRAECHHDSGHGYLDHKDEYLRRLRRIEGQARGL
                     QRMVDEEQYCIDILTQVSAMTKALEGFALAMLDEHLSHCVVRAARAGGAEAEEKLAEA
                     SAAIARLVRS"
     gene            7615..7821
                     /locus_tag="PKJ61_10785"
     CDS             7615..7821
                     /locus_tag="PKJ61_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016668944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HOC14347.1"
                     /translation="MLATYTVTGMTCNHCVHAIKEEVAAVPGVTGVELTLEDAKLNVT
                     SDAPVDFDRIMEAVYEAGENYSVA"
BASE COUNT         1329 a         2601 c         2611 g         1294 t
ORIGIN      
        1 ggctggttct gcagcagttc gagggcgcgc agggctcggg cggtggggct ggtctcggcc
       61 attgcgggct acctaatcct gcgtatctct ctgttgggta attccggaag tagatcgtcc
      121 ggattcgatc ctagcttcga gccatgacct accaattgat ccagtcccgg cattcagcgc
      181 catcgctacc gcgaagcctt tccgccgcgc tggcggcgct gcgaagcctc cttgccaagg
      241 tggcgcatcg cctcacgttg cccacccagg tgttcgacga tcccgacttc ggccgattcc
      301 gcggctggcc cgaatcggag gtgcagttgc ggcgatggcc atactgatcc catcgcgtcg
      361 ggcagcggag ggacggatgg cagcggtcgg agggatggag ttagaattca ccgcggacgt
      421 gatcgagtgg cgcggcccgg ccccgttcgt ctacgcggtg gtgcccgagg ccgagggcga
      481 agacatcgcg gccgtggccc gggcgctcac ctacggctgg ggcatggtgc tcgtgaccgc
      541 gacgatcgga gccacccggt tcaccaccgc gctctacccg aaggacggcg gctaccggct
      601 accgatccgg gtggtcgtgc agcgcgcgga gtccatccgg gtgggcggac gggttcaggt
      661 gaggctcgag ctgggcacgc catgagtgct gtcgcggttg accgtccgtt gccgaagctg
      721 gtgctttgat ggacggatgc agcggctgct gagcgcactc gaccggatga accgggcgca
      781 cccgtggtcg cacaacgacg cctatgccgg cttggtgctg caccaagcca ggaagaccca
      841 acgtggcggc ggcagttcgg cgctcgacgt cggctgtggc actgggcggt tgttgcgccg
      901 gttggcggag tgcttcccgc aggcagtggg catcgaagcc gatgctgtga ccgctgggct
      961 ggcggccgcg gcggtggcta cgtttccggc agcgaccgta gtcaacgcca ggtttccagt
     1021 agcggacggt cgtcgctacg acttcgtctc gatggtcgcg gtgttgcacc atctgccctt
     1081 ggcggacggg atcgcggccg tccgcgaagt cgtggctccc ggcggtcggc tggtgatcgt
     1141 gggggtctac cgcgaggcgc gggccgatgc gttgttctcg gtgatctcgc tgctactcaa
     1201 tccgctgatc gggatgatcc tgcacccgcg acctgccgac cggacgctgg agcagctgcg
     1261 cgcaccggct atgcctgcgt ccgagcctta ccgggagatc cgcagcgcgc tccgtgccgc
     1321 gctgcccgga gtgcgggttc gtcgcggatt cttctggcgc tatgtcgcta cctggcaagc
     1381 gccgcgcact tagcccgaac ccaccggccg agcttgtgga gaccggggat tcagcgccga
     1441 agtgactgcg ctcaccgaac acgttggcgg aaatcacgat gctgcgcgag cctgcgtagg
     1501 atcgtccgcg taaagcgcgt cctggtcggt gtggcagcct tggtgctcgt ggttggagca
     1561 gttcatacgg ccttcgagta cggccgaatg gtcgctacgg ccgagtacaa ctccgctccg
     1621 tccagtgtcg cgctgttctt catggtgccg catctggcgg tcagcatgtt gctgctggta
     1681 gcggctgtgt tcacgccacg gcgcaagcgc gcaaacgaac ccgcggacac gcgctcaccg
     1741 cgctgacggc caaaggtcga cctcgcggct caggcggggc tgcggtggtg cctggccact
     1801 gactgtgact gcacccaccg cggacggtgc actcagccac agcctcgacg attcggacgg
     1861 gaatcagcgc agccgggccc gatcggcaag ggtgttcagc agcgcgacgc cagtggcggc
     1921 atcgtcgact gtgatcacca attgccgtcc gccggtgcgg tgcacgagga tcgcctcgcc
     1981 cttgcgcagc acgatgccga cgcgtccggt gagatcgatc cggtagcccc agccgccgaa
     2041 gtccgccaat ggctggacgt cgaccacttc ggccgcttcc acctcgttta gcggcaccac
     2101 cgtccgcggc cgtggcagta ccgagcgcgc ggccaggccg tcgtggtcga cggtgaccgt
     2161 ccagcggccg agcagcacca gcaacaacgt gaccatagcc aggatgacca gggtcgtcac
     2221 gccgaccccg acaccaccga gcagcgcgat gccgcccagc aggacggtca ggatcgcccc
     2281 ggcggtcatg atccagccca gtccgggcag ttccgccgtc cgcacccagg ccgcctgctc
     2341 gttcgcgccg agcgtcaggc gctccgcgcc gtccggtacc ggggcggtgg tgggctgcgg
     2401 ccggtccgcc gggatggcga gcgcggccag cgcgccggcc agggttgcga cagcgaagga
     2461 aaccgccatc ggtaccccga ttccgggcgc gagggtggcg tcggtcaagc cgcgctgcac
     2521 ggccaacgag ccgaccagca gaccgctgat catcaccgcg aaccagatcg agaagaacac
     2581 cgacaaccgg cgagtggacg ctgtcgagcc caggtagaaa ccgatcgccc acatgccgat
     2641 cgtgaacggc acgacgatcg cggccagcat caccagcgtg ctcaccgggc taccgaaacc
     2701 gtccggcagg ccgtccgcac cccagtgggt agccacctga tcgggcagtt cgggcagcat
     2761 cgcgaccgcc cacgccagcg cggcggccag caggacgagc ggaaccacga gcgcaagcac
     2821 cgtagtccgg acgcgattcg gcaccttggc aggtctggac gtcatcgcag ggtctccttc
     2881 aacaaagcga tcgtggtttc ggccgagagc tccagccgac gggactcggt ggcgaccgcc
     2941 gcgagggcgc ggcgcagcgg ggccaggtct tgggtggcac gctcggtgac cacggcgccg
     3001 cggccgcggc gaagctcgat caggccttcg tcgcgcagtt cctggtaggc gcgcagcact
     3061 gtatgcaggt tcacgttcag agacgcggcc agctcgcggg ccgccggcag ccgttcgccc
     3121 gagcgcagtg aaccgtccgc cacgccggcg cggagttgcg cggcgagctg agcgaacagc
     3181 ggctcggtgg ccgacgggtc gattcggaaa agcacaggtt cagtatagtt gttatatgac
     3241 aactagaaca gttacttcca ctcagttcta gaaccgctga tcaacgcggc ccaacgctgg
     3301 ctgagcctga cgaagcccgt agtcaccccg cggatggacg acccaacgcc cggctcaggg
     3361 atcgaaatca gcgcttcgct agtcgagcgc ggcgagcaag cggacggccc gcttggcaac
     3421 cgcgggcagt cgcgacttcg agtgtcgctg cagtcgttcc cggagcgccg gacgcagcga
     3481 agtgtcggcg gcggccaacg tggccagagc cgacatcgcg caggtgagca cgatccagtc
     3541 gtcggcatcg tcgagggtac ggatcagcca ttccgcggcc cgcgcgcgtt gggcggcagt
     3601 gagcggcacc tcgccccaga gttgggccag gtgccactga atcgacggct gggtgctggc
     3661 cgccagttcg gcgtccaggc gatcgacgaa gggcgtcagc agttccggac gctcacgcgc
     3721 gaacttctcc agcgcgtccg cagcccgcat ccgcacgacc gcgtccgcgg ctgtacagca
     3781 gtcgatcaac tcgctgatcc gagacggatc ggcccgcagt tcggcgagca cttcggtcac
     3841 gccgagcagc gtccgcgggt tgccgccggt gagcatgtcg acaaacgccc tacccgcggg
     3901 caccttcgcc accagcgtcc gcggcgcggt gaaccggtac gaggcctcga tccagtcggc
     3961 gagttcggcc aggtcggtgt gctcgtcgag gtcgagtccc gtccagccgg acgggccgag
     4021 atagtcggga ttgtacgcgc aatcctgcgc ccggaggatc gagatggtct ccagatcgcc
     4081 gaccagcacc accgattggg aatgccgcac ccaggctccg tccaccttca gcgagccgcc
     4141 gtagtaggcg aacaccttct tcgtgtagaa ggtcggacgc ccatggctca ccttctcctg
     4201 cgctcccggc agcgcgagcg ccaagccccg gacccgctgg taccaggggt cggcctcgtc
     4261 gaacatcagc ggatgctcca ccggttcagc cctacgccca acagcgtccg gctaggagac
     4321 gcagaggcag aaggggtgcc cagccggatc gaggaagacc cggaaggtct cgcctggctg
     4381 gtgttcggcc ttgtgggcgc cgagttccag ggtcgcggcc gcggctgcgt ccaggtcgtc
     4441 caccacgaag tcgaggtgtg actgctgcgg cacttcctgc ccgggccaga ccggcggacg
     4501 gtagtccgcg acctgttgga agcagatcgc ctgcccgtag tcggcgcgga cctcggccca
     4561 gtcgccgtcg agctggacct tccagtccag gagccttccg tagaactcgg cgaggacggc
     4621 gggatcgggg cagtccagca ccacgctggg gaatcgtgcg atagccatgc cggagcctaa
     4681 ccggacaccg gacgctcggc acctggtgag aagtgcggta cctcgactcg tggcactgca
     4741 actgtcctag gggatgaggg tgttctcggc gacggccagg tccagctctt ctgcggccgt
     4801 gagaagccgc cgatcacggg tccagagacg agccgctggc gtgagccgcg tggccgcgag
     4861 caggtggacg tccaccaggc tcaggccgcg cgagtacagg gcctgagcct cgatgaacgc
     4921 gagaatttcg gcatcggacg cggacgcggc gtgagggagg tgcgccagca gtccgagcac
     4981 cgtgtctcga tcggccaggg agcccagcgc cagctcgccg gtcaccagcg ggtgggagag
     5041 cacctcgttg cgattcagca gcgcaaccaa ccggggctcg ccctggtgca ggtggtctat
     5101 ccagatcgag gtgtcaacca ggatcacgtt tcgctgcccc ggtggcgggg agcggacacg
     5161 gcagctggat ctgtgccgcc aagcctagcc aagcgcctgg cgctctcctg ttggatcagg
     5221 gcttcaaggc ccttgcggaa cagggtcgag cgctcactcg tcccggtgag ctccgccgcg
     5281 agcgcgagca gatcgctgtc gatggtaact gtggtgcgca tggtgacctc ctgcatcacc
     5341 ctcagcatag tttgatgctc aatctgatgc gaaagggtca gtgctgcatg tccacgaagc
     5401 gggagtagtg gccctggaac gcgacggtga cggtcttggt ctggccgttt cggtgcttgg
     5461 caacgatgaa gtcggcctcg ccggtccgct cggacgagtt gttgtacatg tcgtcgcggt
     5521 gcaacaggat caccacgtcc gcgtcctgct ccagcgaacc ggactcgcgc aggtccgaca
     5581 gcatcggctt cttgtcggtg cgctgctcgg ggccacggtt gagctgggag agcgcaacta
     5641 cgggcacctc gagctccttg gcgagcagct tcatctgtcg ggagaactcc gacacctcca
     5701 actggcggct ttccacccgc ttgcccgaac tcatcagctg catgtagtcg acgatcacca
     5761 gtttgaggtc gtggcgctgc ttgagtcgcc gcgccttggc ccggatctcc atcatggtca
     5821 ggttcggcga gtcgtcgatg aacagtggtg cgctggacac gtcgccggtc ttccgcgcga
     5881 tccgggccca gttctcgtcg ctcatgtggc ccttgcgaat gtcctgcagg ggcacttgcg
     5941 cctccgccga gatcaagcgc atcacgatct cggtcttcgt catttccagg ctgaagaacg
     6001 ccgagcacag tccgtgctga atagacgcgg cccgcgcgag gtcgaggccc agcgtcgact
     6061 tgcccacacc cggacgcgcc gccacgatca ccatctgccc ggcgtgcagc ccgttcgtca
     6121 gctcgtccag atcgacgaac ccggtcggca cgccggccat gatgccgtgc gagctcagtg
     6181 cctcgatctc gtccagcgtg gactccatca gcgcgctcaa cggctcgtag tcgtcgccgg
     6241 ccttgccctc ggccacttcg tagatcgcct gctgcgccgc gtccacgatg ccggcgacat
     6301 cgccctggcc ggcgtagccc aactggctga tcttgatcga cgcctcgacc agccggcgca
     6361 ggatcgcctt ctcccgcacg atctcggcgt agtaggcagc gttggacgcg atcgagacca
     6421 tcgacaacag gtcgtgcagg tagatgtgcc cgccgatccg tccgagttcg cccaatttcg
     6481 ccagctcgtc ggcgaccgtg atcgcgtccg ccggctcacc gcgtccgtac aggttgagga
     6541 tcgcgtcata gatcgtctcg tgtgccgggc ggtagaagtc acgccccttg acgatctcgg
     6601 cgacatcgcc gatcgcatcc ttgctcagca gcatcgcgcc cagcacgctc tgctccgcgg
     6661 caacgtcctg cggcggcgtg cgatcgggcc ctgcgtagga tgcctcttcg tagtcgatga
     6721 cttctgcgag cgacatgact cccctcaaga ctcgtcttca ccactgatcc atgctccgct
     6781 gagcacgtta ggacgagcct cagacacttt ctgtcggccg cctcgcgacg gttccggatc
     6841 gtcgccccgc accttccgac ccgcgccaac ctagacccat ccgggtcacg tctcaatggc
     6901 gctatccaca ggccctgtgg atagcgtggg gacaacgcgc gcgacacgcg cagggggctg
     6961 tgcaaggagt ggtgccgtaa ctgtggaaac ctcgtgaatc gttcagtgaa gcgccatctg
     7021 acaagggaag atgcttcatg cccatgtgga cggaagaagt cgtacagcaa ttttgagacc
     7081 gtcggaccgg acttgacagg cgaagcctgg ccgggtgtat agcggctgcg cactcgcggt
     7141 tatgcacaca ctatccacaa cctctggctt ggcttccata cccatggggg gtatcctgaa
     7201 gagggaggat gaagatgacc gcgaatcacg aagaacaact gtcacaccgg gccgagtgtc
     7261 accacgactc cggccacggg tatctcgacc acaaggacga gtacctgcgc aggttgcgcc
     7321 gtatcgaggg ccaggcgcgc gggctgcagc gcatggtcga cgaggagcag tactgcatcg
     7381 acatcctcac ccaagtgtcg gccatgacca aggcgctcga aggcttcgcc ctggcgatgc
     7441 tggacgaaca cctcagccat tgcgtcgtcc gcgcggcgcg agcgggcggc gccgaagcag
     7501 aggagaagct cgccgaagcg tccgctgcga tcgcgcggtt ggttcgctcc tgacgggtcc
     7561 agctcgacca accgaatcca aagaccaact gaagtccaac gaaaggaaac cgcaatgctc
     7621 gcgacctaca ccgttaccgg aatgacctgt aaccactgcg tccacgcgat caaggaagag
     7681 gtcgccgccg tccccggcgt gaccggcgtc gaactgaccc ttgaagatgc caagctgaac
     7741 gtgacctcgg acgccccggt cgacttcgac cggatcatgg aggccgtcta cgaggccggc
     7801 gagaactaca gcgtcgcctg agccatgagc accca
//
LOCUS       DAOPRQ010000096         7819 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_7737_length_7819_cov_13.431479, whole genome shotgun sequence.
ACCESSION   DAOPRQ010000096 DAOPRQ010000000
VERSION     DAOPRQ010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 7819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7819
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7737_length_7819_cov_13.431479"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            300..1877
                     /gene="glpA"
                     /locus_tag="PKJ61_10790"
     CDS             300..1877
                     /gene="glpA"
                     /locus_tag="PKJ61_10790"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004809824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpA"
                     /protein_id="HOC14348.1"
                     /translation="MRKLAVDVVVIGGGSTGAGVVRDVAMRGYSTVLLDRADLGQGTT
                     ARFHGLLHSGGRYVVSDPLSATECAQENVILSRIQASAIETTGGYFVTCPGDDPEFAD
                     KFLAGASATGVPAQEIPVAQALKAEPRLNPGILRAIEVQDGSVDGWQLVWGAARSAEA
                     YGAQILTYHKVTRITRDGNRVAAVHAIDLKNGEEVQIECNFVLNCAGPWAGQIAAMAD
                     CEPVDVIPGRGIMIAMNHRLVNRVVNRCIYPADGDILVPVHTVSIIGTTDVKVDDPDN
                     LAIELDEVQQMLDAGEILIPGFRQSRALHAWAGARPLIKDKRVAAADTRHMSRGMAVI
                     DHTPRDGLDGLLTIGGGKLTTYRLMAEHIVDAMCAKLGETRACRTADEAIPGSESGKN
                     YLVTHRLEAREEDRFDDQILCECELMNRKMFTDALAEQPKGSFDDLRRQLRLGMGPCQ
                     GGFCTTRATGVALASDHIDVERATGLLRLFLKNRWIGLWPILYGEQVRQTALDNWIFR
                     STLDVEHLPEPVSEVTL"
     gene            1874..3136
                     /gene="glpB"
                     /locus_tag="PKJ61_10795"
     CDS             1874..3136
                     /gene="glpB"
                     /locus_tag="PKJ61_10795"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015583618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HOC14349.1"
                     /translation="MSRVIVIGAGLAGLVAANRLADAGANVTMLTKGLGGLQLGQGTI
                     DVLGYRPQRVTNPLLELSEAFPEAGEGDRVHPYSVIGAKAVHESLAYLLTIVPDLLVG
                     DAEANYQLPTAVGAIRPTCLAQPSMLAGNVAAGKKFAIVGLRQLKDFQPKLIAQNLAR
                     TDLPEGGRVTARDLMLDFAAREPEIDSFGLTYARAFDDPDYRARFAAALKPLLAEGEI
                     VGLPAVIGIKDANAWRDLAERLDHPVFEIPLPPPSVPGMRLNEALTARAKAKGVRWVP
                     GVRTIAYHALDGRVTAVSTATVPAPKEYKADAFVLATGGFESGSLTLDSYGNVTETLF
                     GLPLAGTDRPLVHGDYWGAEQPLFEVGVMVDAQMRPRAAAGVPVYSNLYAAGGIIGGT
                     SRWRELSGDGIALASALKAADAILEELA"
     gene            3133..4365
                     /locus_tag="PKJ61_10800"
     CDS             3133..4365
                     /locus_tag="PKJ61_10800"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004809821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HOC14350.1"
                     /translation="MNSKGLEFAGHEFARASLDACVKCTICETQCPVSAVTPLFTGPK
                     FVGPQAERFRNGESVDHSLDYCSSCGTCTLVCPQGVQIAELNSQARAVMKADHMPLRD
                     RLIGQTTLMGMAMTPVAPIANAALHNGVIRTVMEKVVKVHRNAPMPPAQTQTFESWFR
                     KRTQPSGPFPRGAVVFFHGCAGGYFEVETSKKTVEVLEHLGYEVLVPKQGCCGLAQQS
                     NGLFDQATASVLKLCDQLRSSGRDLTIISSSGSCTGMLKHEAHEIMGVSDERLKDVGT
                     RIRDVMEFLLNLHDSGELPTDFQRIEMTVPYHAPCQLKSQGIGKPALEVLKLIPGLTV
                     VDSEKVCCGIAGTYGLKKEKYDIAQAVGRPLFEMVKETNPNLALCDTETCRWQIEQSS
                     GVKTEHPIWMVHRAYGLS"
     gene            complement(4775..5587)
                     /locus_tag="PKJ61_10805"
     CDS             complement(4775..5587)
                     /locus_tag="PKJ61_10805"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOC14351.1"
                     /translation="MSLAFDMASRAKWGGRAQAYAETFAGQCAHAISPMLDALDLQPG
                     ERLLDVGTGAGAVAVAALVRGVRVTAADPDADMLALAAVAAPDAVLRQGALPDLDLGH
                     GDFDAITANFVLNHVGDPGASARRLGELLRPGGRLGVSIWPAERGPLFRIWDEVVAEA
                     GVSDLSKATLPAHLEFPRTPDGLNGLLSAAGLSVREAWLHDFEHVADPDLWWSAAERG
                     VASIGQVYLARPADGQAAMREAWRRVSTHYLDADGRLRLHGTALIAAAARSE"
     gene            complement(5592..6395)
                     /locus_tag="PKJ61_10810"
     CDS             complement(5592..6395)
                     /locus_tag="PKJ61_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016669913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HOC14352.1"
                     /translation="MLILLSPAKALDFESPIPYLKTTWPRLLNESVQLVDVMRDKSVA
                     ELAKLSGISDELAALNAQRWADFSLPMKRSNARQALLAFDGDVYRQLGVRGRFQPRDF
                     TESGKVLRILSGLYGLLRPFDLIQPYRLEMGTRLETVRGKNLYDWWGTLVTDLLAEDI
                     PGSPGAPVVVNLASGEYSGVLQPERLGVRMISPRFEDVDARGRRSVVSFYAKRARGEM
                     AYWLVRNRTYTPVVLPGFVGLGYRYDKAASTPDAPVYVRAFEDRPRPGA"
     gene            complement(6430..6783)
                     /locus_tag="PKJ61_10815"
     CDS             complement(6430..6783)
                     /locus_tag="PKJ61_10815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14353.1"
                     /translation="MNILVGILVVLHLLGWAVALGAIVANFKQPKVASAVSHGLYLAV
                     GAGLVITGIAGAMQWNLNYAKIGIKLVVAVVATVMAVVGQKRPEKITTGYLAGLVGLI
                     VTNVALAVLWRAPED"
     gene            complement(6901..7293)
                     /locus_tag="PKJ61_10820"
     CDS             complement(6901..7293)
                     /locus_tag="PKJ61_10820"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HOC14354.1"
                     /translation="MTLVVDASVVVAALIDSGNAGAWADQVLIDEHLLAPDLLLVEVT
                     NILRRLECSGQLPTPTASLAQQQLLALPVEAVPFAPCAERIWELRRNLTGYDAWYVAA
                     AELASAPLATLDLRLASAAGIRCRVLSP"
     gene            complement(7290..7526)
                     /locus_tag="PKJ61_10825"
     CDS             complement(7290..7526)
                     /locus_tag="PKJ61_10825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14355.1"
                     /translation="MTKAVTIRDLPDAVVAELASRAAADGRSLQEFLKAQLVGLAARP
                     DINHWVAEARGRKQVVGVTLEIDDILTARDADRR"
     gene            7638..>7819
                     /locus_tag="PKJ61_10830"
     CDS             7638..>7819
                     /locus_tag="PKJ61_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011758110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOC14356.1"
                     /translation="MSEIREVIIIGSGPSGYAAAIYTARAGLKPLVFEGALAAGGALM
                     NTTEVENYPGFTDGIMG"
BASE COUNT         1330 a         2629 c         2623 g         1237 t
ORIGIN      
        1 actttccctc agctcatgcg aggtgtcggt ccgaactgtg gaagacctga acgaagaggg
       61 aaccgtcccc ctttcagcca gcccctttca atcagccctt cggttcagcc gtccttgaac
      121 gccctgcggg acgcggggcg atttcccacc gtcagggacg caggttgcac gtctgcgcga
      181 cctcgcccgt tgttgctgaa acccgcaccc tgggctgact agcctgcgaa gggaacgcgc
      241 gctgcgcgct gcccggacgg cgaggtcggc gtccgcgtcc cagctatagg gaggtcaacg
      301 tgaggaagct ggctgttgac gtcgtggtca ttggcggcgg ctcgaccgga gcgggcgtgg
      361 ttcgcgacgt ggcgatgcgc ggctacagca cggtgctgct cgaccgggcc gacctgggtc
      421 agggcaccac tgcccgattc cacgggctgc tgcactccgg cggacgttac gtggtcagcg
      481 acccgctgtc agccacggaa tgtgcgcagg agaacgtcat cctctcccgc atccaggcgt
      541 ccgcgattga gaccaccggc ggctacttcg tcacctgtcc cggcgacgac ccggagttcg
      601 ccgacaagtt cctggccggc gcgtcggcga ccggcgtccc ggcgcaggag atcccggtcg
      661 cccaggcact gaaggccgag ccgcggctca acccgggcat cctgcgcgcg atcgaggtgc
      721 aggacggctc ggtggacggc tggcagctcg tctggggtgc ggcccgctcc gcggaggcct
      781 acggggcgca gatcctgacc tatcacaagg tcacccggat cacccgggac ggaaaccgcg
      841 tagcggccgt gcacgccatc gacctcaaga atggcgaaga ggtgcagatc gagtgcaact
      901 tcgtgctcaa ctgcgccggt ccgtgggccg gccagatcgc cgcgatggcc gattgcgagc
      961 cggtggacgt gatccccggg cgaggcatca tgatcgcgat gaaccatcgc ctggtgaacc
     1021 gggtggtcaa ccgctgcatc tacccggcgg atggcgacat cctggtgccg gtgcacaccg
     1081 tcagcatcat cggcaccacg gatgtgaagg tcgacgaccc cgacaatctg gccatcgaac
     1141 tagacgaggt gcagcagatg ctggacgccg gcgagatcct gattcctggt ttccgccagt
     1201 cccgggcact gcatgcgtgg gcgggcgcac gtccgctgat caaggacaag cgcgtcgccg
     1261 ctgccgacac ccggcacatg agccgcggca tggcggtcat cgaccacact ccccgcgatg
     1321 ggctggacgg gctgctgacc atcggcggcg gcaagctcac cacctaccgg ctgatggccg
     1381 agcacatcgt ggacgcgatg tgcgccaaac tcggcgagac ccgcgcctgc cggacggcgg
     1441 acgaggccat ccccggctcg gaatcgggca agaactacct ggtcacccac cgcctcgaag
     1501 cccgcgaaga ggaccgtttc gacgaccaga tcctgtgcga gtgcgaactg atgaaccgga
     1561 agatgttcac cgatgcgttg gccgagcagc cgaagggctc gttcgacgac ctgcgtcggc
     1621 agctgcggct gggcatgggc ccttgtcagg gcgggttctg cacgacccgg gcaactgggg
     1681 tcgcgctggc gtccgatcac atcgacgtcg agcgggccac cggtctgctg cgactgttcc
     1741 tgaagaaccg ctggatcggt ttgtggccca tcctgtacgg cgaacaggtg cgccagaccg
     1801 cactcgacaa ctggatcttc cgatccacgc tcgacgtcga acacctgccc gaaccagtca
     1861 gtgaggtgac actgtgagcc gcgtcattgt gatcggggcc ggccttgccg gcctggtggc
     1921 tgccaaccgc ctggcggacg cgggggcgaa cgtcaccatg ctgaccaagg ggttgggcgg
     1981 gcttcagctc ggccagggca ccatcgatgt gctcggctac cgcccgcagc gggtgaccaa
     2041 cccgctcctg gagctcagcg aggcgttccc tgaggctggc gaaggcgacc gggtgcaccc
     2101 ctattcggtc atcggggcca aggcggtcca cgaaagcctg gcgtacctgc tgacgatcgt
     2161 ccccgacctc ctggtcggtg acgccgaggc caactaccag ttgccgactg cggtgggcgc
     2221 gatccgtccc acctgcctgg cgcagccgag catgctcgcc ggcaacgtcg ccgccgggaa
     2281 gaagttcgcg atcgtcggcc tgcggcagtt gaaggacttt cagcccaagc tgatcgccca
     2341 gaacctggcc cgcactgacc tgcccgaagg cggacgggtc accgcgcgcg acctgatgct
     2401 cgactttgcc gcgcgcgagc cggagatcga ctcgttcgga ctcacctatg cccgcgcgtt
     2461 cgacgacccc gactaccgcg cccggttcgc ggccgcgctg aagccgttgc tggccgaggg
     2521 tgagattgtc gggctgccgg ccgtcatcgg catcaaggac gccaatgctt ggcgcgatct
     2581 agccgagcgg ctcgaccacc cggtgttcga gatcccgctg ccgccgcctt cagtgccggg
     2641 catgcggttg aacgaagcac tcaccgcgcg tgccaaggcg aagggggtgc gttgggtacc
     2701 cggcgtccgg accatcgcct accacgccct ggatggccgg gtgaccgcgg tcagcaccgc
     2761 gaccgtcccg gcgcccaagg agtacaaggc ggacgcgttc gtgctggcca ccggtggatt
     2821 cgagtccgga tcgctgacgc tggactcgta cggcaacgtc accgagaccc tgttcgggct
     2881 gccgctggcc ggcaccgacc gtccgctcgt gcacggtgat tactggggtg ccgagcagcc
     2941 gctgttcgag gttggcgtga tggtggacgc gcagatgcgt ccgcgcgcag cggccggggt
     3001 accggtgtac tcgaacctgt acgccgccgg cggcatcatc ggcggcacgt cgcggtggcg
     3061 cgagttgtcc ggggacggca tcgcgctggc cagcgcactc aaagcagccg atgcgattct
     3121 ggaggagttg gcatgaacag caaaggcttg gagttcgccg gccacgagtt cgcccgggcc
     3181 agcctggacg cgtgcgtcaa gtgcacgatc tgcgagacgc agtgcccggt ttcggcggtg
     3241 acgccgctgt tcaccggccc caagttcgtc ggtccgcagg ccgagcggtt ccgcaacggc
     3301 gagtcggtcg accactcact ggactactgc tccagttgcg ggacgtgcac gctggtctgc
     3361 ccgcagggcg tccagatcgc cgaactcaat tcgcaggcgc gtgcagtgat gaaggccgac
     3421 cacatgccgc tgcgcgaccg gctgatcggg cagaccacgc tgatgggcat ggcgatgacg
     3481 ccggtggcgc cgatcgcgaa cgccgcgctg cacaacggcg tgatccgtac ggtgatggag
     3541 aaggtggtca aggtgcatcg gaatgcgccg atgccgccgg cccagaccca gacgttcgaa
     3601 agctggttcc gcaagcggac gcagccgtcc ggaccgttcc cgcgtggagc ggtggtgttc
     3661 ttccacggct gcgcgggcgg ttacttcgag gtggagacgt ccaagaagac cgtcgaggtg
     3721 ctcgagcacc tgggttacga ggtgctggtg cccaagcagg ggtgctgcgg cctggcccag
     3781 cagtcgaacg ggctgttcga ccaggccacc gcctcggtgc tgaagctctg cgaccagctg
     3841 cggtcgtccg gtcgcgacct gacgatcatc tcttcgtccg ggtcgtgcac gggcatgctc
     3901 aagcatgagg cgcacgagat catgggcgtg tccgacgagc gattgaagga tgtggggacg
     3961 cggatccgcg acgtgatgga gttcctgctc aacctgcatg actccggaga gctgccgact
     4021 gacttccagc ggatcgaaat gaccgtgcct taccacgcgc cctgccagct gaagagccag
     4081 ggcatcggca agccggcttt ggaggtgctg aagctgattc ccggcctgac cgtggtcgat
     4141 tcggagaagg tctgctgcgg catcgccggc acttacgggc tgaagaagga gaagtacgac
     4201 atcgcgcagg cggtcgggcg tccgctgttc gaaatggtca aggagaccaa cccgaacctc
     4261 gcattgtgcg acaccgagac ctgccgctgg cagatcgagc agtcctccgg cgtgaagacc
     4321 gaacacccga tctggatggt gcaccgcgcg tacggtctga gctgaatctg ggcgctggtg
     4381 aggcggcgac tccaccggag ggtcactcgc cgatctacgg ctggcctcgt cccgattcgg
     4441 agtgacggtc atctcaggtc gtactcaacc cagttttggc tggttatggc ggggggatgg
     4501 gctgcctgta ctgagtcgac ccagttttga ctggttgtga ggccagaatt gccgtccata
     4561 gccggtcgaa agtgggttgt tcccgtgggc tgctccagcg ggcgcgtcat agccggtcaa
     4621 aactgggttg aaccacgaat tgccgctgac tgcggtcccg aacgagtgtc cggggaggtg
     4681 ctgggagatc ccggccttcg ccgggatgac gtcttagttg ttccgggacg acgttttagt
     4741 cttcgtcgct ctggtgctcg actgatgcct ggtgctattc gcttcgcgct gccgcggcga
     4801 tcagagctgt gccgtgcagg cgcagccgcc cgtccgcgtc gaggtagtgg gtactgaccc
     4861 gccgccaggc ttcgcgcatg gccgcttgac catcggccgg ccgggccagg tagacctggc
     4921 cgatactggc cacgccacgc tcggctgcgc tccaccagag gtccgggtcg gcaacgtgct
     4981 cgaagtcgtg cagccacgct tcgcggacgc tcaggccggc ggcggagagc agcccgttca
     5041 gcccgtccgg cgtccgtggg aactcgaggt gggcgggcag cgtggccttg gacaaatcgc
     5101 tcactccggc ttcggcgacg acctcgtccc agattcggaa gagcgggccg cgctcagccg
     5161 gccagatcga aacccctagt cggccgcccg gacgcagcaa ctcacccagg cgacgagcgg
     5221 acgcccctgg atcgccgaca tggttgagga cgaagttggc cgtgatggcg tcgaagtcgc
     5281 cgtggcccag gtcgaggtcg ggaagcgcgc cttgtcgcag gacggcgtcc ggggcggcaa
     5341 cggctgcgag cgcaagcatg tccgcgtccg gatcggcggc ggtcacccgg acgccacgta
     5401 cgagcgcagc cacggccacc gcgccggcgc cggtacccac gtccaacagc cgctcacccg
     5461 gctgcaggtc cagcgcgtcc agcatcgggc tgatcgcgtg ggcgcactgg ccggcgaacg
     5521 tctcggcgta cgcctgcgcc cggccgcccc acttggctcg ggacgccatg tcgaaggcga
     5581 gggacatggc gtcaggcgcc gggcctgggc cgatcctcga acgcgcggac gtacaccggg
     5641 gcgtccgggg tcgaggcggc cttgtcgtag cgatagccga ggccgacgaa gcccggcagc
     5701 accaccggcg tgtaggtgcg attccgcacc agccagtacg ccatctcgcc gcgggcccgc
     5761 ttggcgtaga acgacaccac gctgcggcgt ccacgagcat cgacgtcctc aaaccgcggc
     5821 gagatcatcc tgacgccgag ccgctcgggc tgcagcaccc ctgagtactc gccggaagcc
     5881 aggttcacca ccaccggcgc gcccggcgag ccgggaatgt cttcggccag cagatctgtc
     5941 accagcgttc cccaccagtc gtagaggttc ttgccacgga cggtttccag ccgcgtcccc
     6001 atctccaggc ggtagggctg gatcaggtcg aacggacgaa gcagtccgta cagcccggac
     6061 aggatccgca ggaccttccc ggactcggtg aagtcgcgcg gctggaagcg cccgcgtacg
     6121 ccaagctggc gatacacgtc gccgtcgaag gcgagcagcg cctgccgggc attgctgcgc
     6181 ttcatcggca gtgagaaatc tgcccagcgc tgggcgttga gcgcggccag ttcatccgag
     6241 atgccggaga gcttcgcgag ctcggccacc gacttgtcac gcatcacgtc gaccaactgc
     6301 accgactcgt tcagcagccg cggccaggtc gtcttcaggt agggaatcgg actctcgaag
     6361 tccagcgcct tggccggaga cagcaggatc agcacggccg aagcctagcc acgcacgcgt
     6421 cgtccggcat cagtcctcag gtgctcgcca gagcacggcc agcgcgacgt tggtcacaat
     6481 cagcccgacc agcccagcca gatacccggt ggtgatcttc tccggacgct tctggcccac
     6541 gacggccatc acggtagcca cgaccgcgac caccagcttg atgccgatct tggcgtagtt
     6601 gaggttccac tgcatggcgc cggcgatccc ggtgatcacc aacccggccc caactgccag
     6661 atacagcccg tgcgacaccg ccgaggcgac cttcggctgc ttgaagttcg ccacgatcgc
     6721 cccaagcgct accgcccagc cgagcaggtg caacaccacc agaatcccca ccaagatgtt
     6781 catggcgccg agcctacaag ccggcgctga catcgccgtg gtcgccagcg tcgtccctct
     6841 cccaggtctc ggcctggcct cgacggccag ccttctgccg ggatgacggt ggatctgagc
     6901 tcagggggac aacacccggc agcgaattcc ggccgccgaa gccaggcgca gatccagcgt
     6961 cgccaacggt gcggacgcaa gctcggccgc ggccacatac caggcgtcat agccggttag
     7021 gttgcgccgg agttcccaga tgcgctccgc acagggcgcg aagggcaccg cctccaccgg
     7081 gagcgcgagc aactgctgct gggcgaggct cgccgtcggc gtcggcagct gaccggaaca
     7141 ctcaaggcgt cgcaggatgt tcgtcacttc gacgagcagc aagtccggcg caaggaggtg
     7201 ctcgtcgatc agcacctggt ctgcccaggc acccgcgttc ccgctgtcga tgagcgccgc
     7261 cactaccacg gacgcatcga cgaccagcgt catcgccgat ccgcgtcgcg cgcggtcaga
     7321 atgtcgtcaa tctccaaggt gactcccacc acctgcttgc ggccgcgggc ttcagccacc
     7381 caatgattga tgtcgggacg ggcggcgagc cccaccaact gggccttgag gaactcctgc
     7441 aacgaccgcc cgtccgctgc ggcgcgactg gccagctccg cgaccaccgc gtccggcagg
     7501 tctcgaatcg tcaccgcttt cgtcatagca gcaaaatagc agcacaagca gcagattgac
     7561 agacacgccg ggaatgctgt cgctcgcccc gtggttccac ggatagagtt ggcagcccaa
     7621 agaacgccag aggaaccatg tccgaaatcc gtgaagtcat catcatcggc tccggcccgt
     7681 ccggttacgc ggccgcgatc tataccgccc gcgccggcct caagccgctg gtgtttgagg
     7741 gtgcgctggc cgccggcggc gcgctgatga acaccaccga ggtggagaac taccccggct
     7801 tcaccgacgg catcatggg
//
LOCUS       DAOPRQ010000097         7665 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_7929_length_7665_cov_12.435217, whole genome shotgun sequence.
ACCESSION   DAOPRQ010000097 DAOPRQ010000000
VERSION     DAOPRQ010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 7665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7665
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7929_length_7665_cov_12.435217"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(334..3960)
                     /gene="murJ"
                     /locus_tag="PKJ61_10835"
     CDS             complement(334..3960)
                     /gene="murJ"
                     /locus_tag="PKJ61_10835"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HOC14357.1"
                     /translation="MSETQAGGGRRLLNATAVMASGTAVSRVLGLVRAMLIAFVLGNA
                     TMRVDVFNVAMFVPNSLYVLLAGGTLNNVLVPQIVRAVNHDEDGGKAFIDRIITGFLI
                     VLGALTVVFTVATPLVMSIYASRWNTPELADHWRSLLLLSYLTMPQLFFFGVFFLIGQ
                     VLNARETFGPMMWAPIVNNVVSIGVFGVYLWVWGTQTTPGTTFTDPQLWLLGLGSTLG
                     IAAQTVVLLPYLKKAGFSYHPRFDLKGTGLGRTFHVAKWMVGYVALTSLAQVVMTNLA
                     TSGTASTDGASSGAGLTAYQNAYLIWILPHSLLTVSLATAMLPMASRYAAAGDRAGVA
                     AETNRAIRLALTFLLPASVAFVALSDPITRVPFGMGVGANDYHFVAWALAAFGIGLVP
                     YTIQYLYLRAYYALDNTKVPFLLQIWISGANAGLAVVLVLAWHSPETIAARLALAYSL
                     AYVLGVFITHRSLRKRLPDLDGHATWHYLVRLFWATLPAAALAWVVTWWFAQYPSALL
                     RIVGLALAGLLAVLAFFFAAKRLGIPEATELIGVLRRRKGDEGESDAVEAIVEESDQE
                     ADQRQPAQPVAAGVREERTNRHVGPPSPFAPPEDELNLDPDDEPSAASAGDGVGVLTR
                     DASAARGVDGTGVFVPIASAPPPPEDVLDFPEPPATELQPTADASADPGRLLGERYRL
                     EEQLAVRDRTQTWRAHDVVLGRPVLVQLLAANDPRTPEALRMAREWAAATDSRFLRVL
                     DVVDSEPGAGAYLVYEYASGQSLEKVLQSGPLTGVETAWIVREVADAMTALHAQGLYH
                     RHLSPATLLVTGSGNVKVLGSGTNGQTESRDGATADVQGLGRVLYACLVKRWPDGDDF
                     GLPAAALVEGRLPSPAQVRSGVAPALDQVADRVLAGVPRGNATRITTAAELTTELSLV
                     LGPMSAAADLSARLHPAETEPPPAAVSTIAPPVPTPVASRFSASARLPEPVADVDYDD
                     DYGDETEPFVDSVLSRSESFTPVPPPPVPAEAEAEAPRRRVPRNSLLIGVIGLVLAVG
                     LIAAMMLSTRSPQPQKAAAPVASAVQIAKADDFDPKADGGSGAENPKQAKLAIDGDPA
                     TAWTTERYRKLAALGGLKPGVGLVLDLGKERTLTQLTLELEGGGTDLEVRVPKDPGED
                     SPMKSNEQWTVVAATRDAAGRAQLDLPGSPKSRYWLVYLTSLPGVEDGYFRGGIAEIE
                     ALGY"
     gene            complement(3957..5999)
                     /locus_tag="PKJ61_10840"
     CDS             complement(3957..5999)
                     /locus_tag="PKJ61_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14358.1"
                     /translation="MIGGTLGWTRRALALLLAFGAGVLALTFGPAAPASAATLAVTVD
                     ITGIAVAGTEPTDLVTLTGTITNTGDVPAYGVQVYLWRSMDPIRDLAALRQTSTNAAG
                     WGTQMPQRPGPYLLVTNSVTAFTPGASAPITLQATLADLGFNTRGAAYAIGAQVLATD
                     DMSSDYQTVGQFRTVVALPAADPVPVTSIVLLSATPTKLVENLFRNDDLAAELSGRLD
                     VLLTAAAQPGMSWLIDPGLLDEVRDLADGYQVGTGTKAGEGAGAAVAAAWLARFATLD
                     PQAGGRTLYANPDVNGARVAADASVVPRAQAAAAAVEGVDDLPLIVVPGGRTLNQSTY
                     AFLADSGANAILASNSAKAGALKRGTAEPLVLTTSALPTTGTDVARTALQQVQETLSG
                     AVVAGQSGVARLLSDEAEVAQDAAAMTGWMQRRRLGDLLTSEPSGAIKLTSTKPARLS
                     KAQFGAIDRLAEDFETYTELVPTTTLTEGAQAALSRTAASAFIGRTDAFDSQARGLAD
                     LVSRPAISRSVTLDASPRFLMSARSNEFPVTVTNKLAEPIRVKVVVKTENPQRLTVPA
                     SQLFTVPPDQSVTVNIKPQATANGLIMANAHVATESGGRVTPDTAIVVEVTDLGVVAW
                     IIVGVSAVVLVGATAWRIRQVRRRDSAQQASAAAEVPAPDTNEQPTASGRTVGKDA"
     gene            6081..7526
                     /locus_tag="PKJ61_10845"
     CDS             6081..7526
                     /locus_tag="PKJ61_10845"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016668677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HOC14359.1"
                     /translation="MPNLTEAQRAALDTVLAAGPKVRRLTDSFAKAGYELYLVGGSVR
                     DAILGRLGNDLDYTTSARPDEIEVLVRKVTKAVWDVGRAFGTIACRITEDDGTDWLVE
                     ITTFRADTYSDHTRKPEVAFGDHLGGDLVRRDFTVNAMALRTTDGELLDPFGGMSDLV
                     AEVIRTPAAAELSFSDDPLRMLRAARFASQLGFVPAPEVVTAMRAMAPRLEIISAERI
                     RDELVKLLLTDDPRPGLNLLVHTGIADVVLPELPALRMERDEHNRHKDVYEHTLTVIE
                     QAIALERARGHQPDLINRLAALFHDIGKPRTRRFEADGKVSFHHHDVVGAKLTRKRMQ
                     ALHFSNEEIKAVAKLVELHLRFHGYGDGQWTDSAVRRYVRDAGAELEHLHILTRSDCT
                     TRNVAKATRLRRAYEELEWRIDELAAQEELDSMRPDLDGTQIMALLGIGPSPVVGEAY
                     KFLLERRIEDGPLGPERAEQELLDWWSVRNA"
BASE COUNT         1213 a         2590 c         2692 g         1170 t
ORIGIN      
        1 gctgttctcg gttgggtcga ctgtgtcgac ctcgatcgga gcgacaactg caacggaccc
       61 gtcgctctag catcctcggc gtaggccgga gcagctagtt gactgctggc gaacatgaac
      121 gcgagggaga tgactcctat cgcgacgctt ctggtcaggg gtggttgccg gtatctcatg
      181 gttggctacc tagggtggtg gggtgtcgcc gaccgggacc ggtcggcgac accgggcgct
      241 agcagtccag ggccgggaat ggtgcgattt ccaagaccaa ggtgtatgga ccaacccgaa
      301 ctctcaagaa atccggagga agtggacggc ggctcagtag ccgagggcct cgatttcggc
      361 gatgccgccg cggaaatagc cgtcctccac gccgggtagc gaggtcaggt agaccagcca
      421 gtaccgtgac ttggggctgc caggtaggtc gagctgggcg cgtccggcag cgtccctggt
      481 tgcggcgacc accgtccact gctcgttcga cttcatcggg gaatcttcac cggggtcttt
      541 cggcacccgc acctccaggt cggtgccgcc gccttcgagc tcgagcgtga gctgggtcag
      601 cgtccgctcc ttgccgagat cgagcaccag gccaacgcct ggcttcagcc cgcccagcgc
      661 ggccagcttg cggtagcgct cggtcgtcca ggccgtcgcc gggtcgccgt cgatcgcgag
      721 cttggcctgc ttcgggttct ccgcgccgct gccaccgtcc gccttcggat cgaaatcgtc
      781 ggcctttgcg atctgcaccg cggacgcaac cggcgcggcg gccttctgcg gctgcggcga
      841 gcgggtgctg agcatcatcg cagcgatcag cccgaccgcc agtacgagac cgatcacccc
      901 gatcagcaat gagttccgcg gcacccgccg gcgtggcgcc tcggcttccg cctcggcggg
      961 caccggcggc gggggcaccg gcgtgaacga ctccgaccga ctcagcaccg aatccacgaa
     1021 cggctcggtc tcgtcgccat agtcgtcgtc atagtcgacg tcggccaccg gctccggcag
     1081 ccgagcggac gcgctgaacc ggctcgccac cggtgtcggc accggcggcg cgatcgtcga
     1141 aaccgctgcg ggcggcggct cggtttccgc gggatgcagc cgcgcggaca ggtccgcggc
     1201 tgcgctcatc ggtcccagca ccagcgacaa ctccgtggtc agttccgcag ccgtcgtgat
     1261 tcgggtcgcg ttcccccgcg ggacgccagc cagcacccga tcggccacct gatcgagcgc
     1321 cggcgcgacc ccggaacgaa cctgcgcagg gctgggcaac cgaccctcca ccagtgcagc
     1381 ggccggcagc ccgaagtcat ccccgtccgg ccaccgcttc accaggcacg cgtacaacac
     1441 ccgacccagc ccctggacgt cggccgtcgc accatcgcga gactcggtct ggccgttggt
     1501 gcccgaaccg agcaccttca cgttccccga cccggtcacc agtagcgtgg ccgggctcag
     1561 atgccggtgg tacagcccct gcgcgtgcag cgcggtcatc gcatccgcaa cctcgcggac
     1621 gatccacgcg gtctccaccc cggtcagcgg tccgctctgc aacaccttct ccaacgactg
     1681 cccggacgcg tactcgtaca ccaggtaggc accggcaccg ggctcggagt cgaccacgtc
     1741 cagcaccctc aggaagcggg aatcagtcgc ggcagcccac tcgcgggcca tccgaagcgc
     1801 ttccggcgtc cgcggatcgt tggcggccag caactgcacc agcaccggcc ggccgagtac
     1861 gacgtcgtgc gcgcgccagg tctgggtgcg gtcgcggacg gccagctgct cttccaggcg
     1921 gtagcgctcg ccgagcagcc gtccgggatc ggcggacgcg tccgcggtcg gctgcagctc
     1981 ggtggctggc ggctcgggaa agtcgagcac gtcttcgggt ggtggcggtg cgctcgcgat
     2041 cgggacgaag acgccggtgc cgtctactcc tcgggccgct gacgcgtccc tcgtcaagac
     2101 tccgacgcca tcaccggctg acgccgctga tggctcgtcg tctgggtcga ggttgagctc
     2161 gtcctcgggt ggggcgaacg gcgagggtgg cccaacgtgg cgattcgttc gttcctcgcg
     2221 aacccccgca gcgaccggtt gggctggttg gcgctggtca gcctcctggt cggactcttc
     2281 gacgatggcc tccaccgcgt cagactcgcc ctcatcgccc ttgcggcggc gcagcacgcc
     2341 gatcaactcg gtggcctcgg gaatgcctag ccgcttggcg gcgaagaaga aggccagcac
     2401 ggccagcagt ccggccagcg ccaggccgac gattcgcagc agcgcgctcg gatactgggc
     2461 gaaccaccag gtgaccaccc acgccagcgc ggccgcgggc agggtggccc agaacaggcg
     2521 caccaggtag tgccaggtcg cgtgcccgtc gagatccggc aggcgcttgc gcagcgaacg
     2581 gtgggtgatg aacacgccga gcacgtaggc gagcgagtac gccagcgcca gccgagcggc
     2641 gatcgtttcc ggactgtgcc aggccagcac cagcacaacg gcgagcccgg cgttcgctcc
     2701 gctgatccag atctgcagca ggaacggcac cttggtgttg tcgagggcgt agtaggcacg
     2761 caggtacagg tactggatcg tgtagggcac caaaccgatt ccgaacgcag ctagcgccca
     2821 cgcgacaaag tggtagtcgt tcgcgccgac gcccatgccg aagggaacgc gggtgatcgg
     2881 gtcggacagt gcgacgaagg ccaccgaagc cggcagcagg aaggtcaggg ccagacggat
     2941 cgcccggttg gtctcagccg ccaccccggc ccggtcgccg gccgccgcat accgggacgc
     3001 catcggcagc atcgcggtgg ccagcgagac ggtgagcaaa gagtgcggca ggatccagat
     3061 caggtaggcg ttctggtagg cggtgaggcc ggcccccgac gacgcgccgt cggtgctcgc
     3121 cgtcccggac gtggccagat tggtcatcac gacctgcgcg agcgaggtca gcgcgacata
     3181 gccgaccatc cacttcgcga cgtggaaagt gcgacccagc cccgtcccct tcaggtcgaa
     3241 gcgtgggtgg tagctgaacc cagccttctt caggtacggc agcagcacca ccgtctgcgc
     3301 ggcgatcccg agcgtcgaac cgaggcccaa cagccacaac tgcgggtcgg tgaaggtcgt
     3361 ccccggcgtg gtctgggtgc cccacaccca caggtacacc ccgaacaccc cgatcgagac
     3421 cacgttgttc acgatcgggg cccacatcat cggcccgaac gtctccctgg cgttgagcac
     3481 ctggccgatc aggaagaaca ccccgaagaa gaacaactgc ggcatagtca ggtagctcag
     3541 caggagcagc gagcgccagt ggtcggcgag ttcgggggtg ttccaccgcg aggcgtagat
     3601 gctcatcacc aacggggtgg ccacggtgaa cacgaccgtg agggcgccca ggacgatcag
     3661 gaaaccggtg atgatccggt caatgaaggc cttgccgccg tcctcgtcgt ggttgaccgc
     3721 gcggacgatc tgcggcacca ggacgttgtt cagcgtgccg ccggcgagta gcacgtagag
     3781 cgagttcggc acgaacatgg cgacgttgaa cacgtcgacc cgcatcgtgg cgttgcccag
     3841 cacgaacgcg atcagcatgg cgcgcaccag cccgagcacc cgcgagaccg cggtcccgga
     3901 cgccatcacg gccgttgcgt tgagcagccg acgtccgccg ccagcctggg tttcgctcat
     3961 gcgtccttcc cgacagtccg accggacgcg gtcggctgtt cgttcgtgtc cggggccggc
     4021 acttcagctg ccgcggacgc ctgctgggcc gaatcccggc gacgcacctg gcggatccgc
     4081 caggcggtgg cgccgaccaa cacgaccgcg gagacaccca cgatgatcca ggccaccacg
     4141 cccagatcgg tgacctcgac gacgatcgcg gtgtccggcg tgacccgtcc gccgctctca
     4201 gtggcgacgt gcgcgttggc catgatcagc ccgttcgctg tggcctgcgg cttgatgttc
     4261 acggtcacgc tctgatcggg agggacggtg aacagctgcg aggccggcac ggtcaggcgt
     4321 tgcgggttct ccgtcttgac taccaccttc acccggatcg gctcggcgag cttgttggtc
     4381 accgtgaccg ggaactcgtt cgaacgtgcc gacatcagga agcgcggact cgcgtccagg
     4441 gtcaccgaac ggctgatcgc cggtctactg accaggtcgg caagccccct ggcctggctg
     4501 tcgaaggcgt ccgtccgtcc gatgaaggcg gacgcggccg tgcggctcaa cgcagcctgg
     4561 gccccctcgg tgagcgtggt cgtgggcacg agttcggtgt aggtctcgaa gtcctcagcg
     4621 agccggtcga tcgctccgaa ctgcgctttg ctcagccggg cgggcttggt tgaagtgagc
     4681 ttgatcgcac cgctcggctc ggaggtgagc aggtcaccca ggcgtcggcg ctgcatccag
     4741 ccagtcatgg cagccgcgtc ctgggcgact tcggcctcgt cggacaacag ccgtgcgacg
     4801 cctgactggc ccgccaccac cgcaccggac agggtctcct gaacctgctg cagcgcggtc
     4861 ctggcgacgt ccgtgccggt ggtcggcagt gcggaggtgg tcagcaccag cggttcggcg
     4921 gtgccgcgct tgagggcgcc ggctttcgcg gagttgctgg ccaggatcgc gttggcgccg
     4981 gagtcggcga ggaaggcgta ggtggactgg ttgagtgtcc ggccgcccgg cacgacgatc
     5041 agcggcaggt cgtcgacgcc ttcgacagct gcggcggctg cctgcgcgcg gggcaccacg
     5101 gacgcgtccg cggcgacccg cgcgccgttc acgtccgggt tggcgtacag ggtgcggccg
     5161 ccggcctgcg gatcgagcgt ggcgaagcgg gcgagccaag ccgcggcgac ggcggcgccg
     5221 gcgccttcgc cggccttcgt gccggtgccg acctggtagc cgtcggcgag gtcgcgcacc
     5281 tcgtccagca ggcccgggtc gatcagccag ctcatgccgg gctgggcggc cgcggtgagc
     5341 aggacgtcca ggcgtccgct cagctcggcg gccaggtcgt cgttgcggaa caggttctcg
     5401 accagcttgg tcggggtggc gctgagcagg acgatcgagg tgactgggac gggatccgct
     5461 gcgggcagcg cgaccactgt ccggaactgg ccgacggtct ggtagtcgct gctcatgtcg
     5521 tcggtggcca gcacctgcgc accgatggcg tacgcggcgc cgcgggtgtt gaagccgagg
     5581 tcggcgaggg tggcctgaag ggtgatcggc gcgctggctc cgggagtgaa cgcggtgact
     5641 gagttggtca cgagcaggta gggacctggc cgctgcggca tctgggttcc ccagccggcg
     5701 gcgttggtgg acgtctgccg tagggccgcc aggtcgcgga tcggatccat cgaccgccac
     5761 aggtacacct gcacgccata ggccgggaca tcgccggtgt tggtgatggt gccggtgagc
     5821 gtaaccaggt cggtgggttc ggtgccggcg acagcgatgc cggtgatgtc cacggtcacc
     5881 gcgagggtgg ccgcgctcgc cggagcggcc gggccgaagg tgagcgccag tacgcccgcg
     5941 ccaaaggcga gcaggagcgc cagggcgcgc cgcgtccaac ccaaggtgcc gccgatcacc
     6001 gtcccatcgt acgggggtgg acgcaggggc ttcggcaggc tcagccgtcg ttccgctacg
     6061 gcccggtaga gtaaccggtc gtgccgaacc tcaccgaagc ccagcgcgca gccctcgaca
     6121 cggtgctggc ggctggtccg aaggtgcgcc ggctgaccga ttcgttcgcc aaggccggct
     6181 acgagctgta cctggtcggc ggttcggtgc gggacgcgat cctcggccgg ctgggcaacg
     6241 acctggacta caccacgtcc gcgcgtccgg acgagatcga ggtgctggtc cgcaaggtga
     6301 ccaaggcggt gtgggacgtg ggccgcgcgt tcggcacgat cgcctgcagg atcaccgagg
     6361 atgacggcac cgactggctg gtggagatca ccaccttccg cgccgacacc tattcagacc
     6421 acacccgcaa gcccgaggtg gcgttcggtg atcacctcgg cggcgatctg gtacgccgtg
     6481 acttcaccgt gaacgcgatg gccctgcgca ctacggacgg ggaattgctc gacccgttcg
     6541 gcgggatgtc cgatctggtc gccgaggtga tccggacgcc ggctgcggcc gagttgtcgt
     6601 tctcggacga tccgctgcgg atgctgcgcg cggccaggtt cgcgtcccag ctggggttcg
     6661 tccccgcacc cgaagtggtg acagcgatgc gcgcaatggc gccgcggctg gagatcatct
     6721 cggccgagcg gatccgggat gaactggtga agttgctgct caccgacgac ccgcgtcctg
     6781 gactgaacct gctggtgcat accggaatcg ctgacgtcgt gctgcctgaa ctgccggccc
     6841 tgcggatgga acgcgacgag cacaaccggc acaaggacgt ctacgagcac acgctcaccg
     6901 tgatcgagca ggcgatcgcg ctggagcggg cgcgcgggca ccagcccgac ctgatcaacc
     6961 gactggccgc actcttccac gacatcggta agccgcggac gcggcgcttc gaggccgacg
     7021 gcaaggtgtc cttccaccac cacgatgtgg tcggcgcgaa gctgacgcgc aagcgcatgc
     7081 aggcgctgca cttctccaac gaggagatca aggcggtcgc caagctggtc gagctgcacc
     7141 tgcgtttcca cggctatggg gacgggcagt ggacggattc agcggtgcgc cgctacgtcc
     7201 gcgacgccgg cgccgagctg gagcacctgc acatcctgac ccgctcggac tgcaccaccc
     7261 gcaatgtcgc caaggccacc cggctccgcc gtgcctacga ggaactcgag tggcggatcg
     7321 acgagctggc cgcgcaggag gagctcgact cgatgcgtcc cgacctggac gggactcaga
     7381 tcatggcgct gctcggcatc ggccccagcc cggtggttgg cgaggcctac aagttcctgc
     7441 tggagcgccg catcgaggac ggtccccttg gcccggagcg tgcggagcag gagttgctcg
     7501 actggtggtc ggtgcggaat gcctagcgaa tggccgcggc cagccggggt cgtgttcgac
     7561 aatgacggac tgctcgtgga ttccgagacc tgctggcacg aggccgagcg gctgctgttc
     7621 acccgtcgcg gcttggcgta ctcagatgag ctgcgcgccg aagtg
//
LOCUS       DAOPRQ010000098         7626 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_7982_length_7626_cov_11.005547, whole genome shotgun sequence.
ACCESSION   DAOPRQ010000098 DAOPRQ010000000
VERSION     DAOPRQ010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 7626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7626
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7982_length_7626_cov_11.005547"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="PKJ61_10850"
     CDS             <1..308
                     /locus_tag="PKJ61_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14360.1"
                     /translation="EQGFTGLKEENHYGDDAVRPPFPGAPSELLPFGEATHADYVELI
                     GIRRRHPWLVDATVTTSDVTNTSIAVHLDSGDSHLTLRLNIGDSAVDGLAPHSWSLT"
     gene            complement(396..1571)
                     /locus_tag="PKJ61_10855"
     CDS             complement(396..1571)
                     /locus_tag="PKJ61_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015071169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOC14361.1"
                     /translation="MDVELARWLVSADARPALAEASRQADPGSLAVATALRRSWPAEQ
                     AAAASSQVELRRRAAGKFGGRAERMFFTPDGLEQATRAEVAAWRASRFVAAGVREVVD
                     LGCGLGADALAFATAGLKVIAVEADPATAVLAAANLDGLGEVICGDAVTLAPQLLANG
                     AAVFIDPARRTASGRTWRVSDFSPPWDFATSLLAGRFGCIKAAPGLPSTLIPDGVAAT
                     WVSHRGDLVETSLWCLTEPRKGRRAVLLPSGAELDAGERREPPSGPIGGYLYEPDPAV
                     IRAGAIGALAEALHARAASPGIAYLFADKLIATPFATAFEVLETLPNDERTLRAWLRE
                     HQIGILEIKVRGLDVDPAVLRKRLKPRGSRSATLILTPTTDGARALVAQRTDARPSK"
     gene            1630..1893
                     /locus_tag="PKJ61_10860"
     CDS             1630..1893
                     /locus_tag="PKJ61_10860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14362.1"
                     /translation="MKVLSKLSLSLGLVALLAGIGVLVWGSWIAYWHYATLSTGRSAE
                     FQDPIPIIAIAAFVIAVGAFLTGLAVAAPRKVYPANQTVDPTV"
     gene            complement(1903..2265)
                     /locus_tag="PKJ61_10865"
     CDS             complement(1903..2265)
                     /locus_tag="PKJ61_10865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14363.1"
                     /translation="MTETPEQAAARIARGASPSSFVAAGGADASEPPETADAAPFDPL
                     RLCIFATIAILGWLFGPLAVVVFAGVGFAGYWKARRAGLTRSKCYLRDTRLVLAYLFL
                     LAVAGVVGLGLRIQDWLI"
     gene            complement(2262..>2473)
                     /locus_tag="PKJ61_10870"
     CDS             complement(2262..>2473)
                     /locus_tag="PKJ61_10870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14364.1"
                     /translation="LLADRVKRITIKPFQDISTMLEERLTQCCVHVGTFSDDGDPQCA
                     PFCAVQAWGRLGRQRVSTARAEPRP"
     assembly_gap    2474..2573
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2574..3557)
                     /locus_tag="PKJ61_10875"
     CDS             complement(<2574..3557)
                     /locus_tag="PKJ61_10875"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOC14365.1"
                     /translation="MTNLLNVTPRTRPSLGPGQPLRGDRIHKYVTAYCPECHDPDAEL
                     SSVQRLSGILVVRDERVWLQRACPTHGMITTLYDEHANILRYLEQWQAPTKVHIPDHA
                     DNFRPMPEAFAHGLPAFHTQHTCILIQDVTEHCNLKCPTCFTASGPALTGVAPLADVL
                     ANVDAKLSREEGRLDVLMLSGGEPTLYPQLAELLDELVARPIVRIMLNTNGLLIASDD
                     ALVELLKSHRDRLEVYLQYDGPSAEASRHHRGADLTRFKEAAIARLSAAGVFTTLVMT
                     AALGVNDGDIGAVVLKALATPFIGGVAIQPVFGSGRGQGIDPMNRLTHTGVL"
     gene            complement(3571..3909)
                     /locus_tag="PKJ61_10880"
     CDS             complement(3571..3909)
                     /locus_tag="PKJ61_10880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14366.1"
                     /translation="MSESTPGWGPTPPPGPPYHLPPQPPRGGSLWLGLAIAVGSMALI
                     WFGVAALSGSGGFDIFWLLFDWPFVLLLGGLVLAAIPRTSRTGGGILLGLGAGVLILG
                     GICIALLASV"
     gene            complement(3902..4690)
                     /locus_tag="PKJ61_10885"
     CDS             complement(3902..4690)
                     /locus_tag="PKJ61_10885"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOC14367.1"
                     /translation="MVGLPFAGSGLVHGLFMGLGILVGVLLFAHELRRRGLKDDRLWI
                     VAGTCLAFGAIGSRLGTWFQFLDLSQNRPVLEWWMDGNRSVLAGLVGAWIGVHVGKLI
                     TGYQASTGDLFAPSVALAMLIGRVGCLLTELPGQPTGGGWGIVLTPEQARLLDGPAGV
                     GLHPSFAYELVFQGLAFWWLLRYRDRLPRSGDLFVAYVTAYALFRFGVEFVRANDQVW
                     GGLTRPQWFLLCTLPLLGWRTSRIWPRTPKTATPIPALNGGSHE"
     gene            4897..5529
                     /locus_tag="PKJ61_10890"
     CDS             4897..5529
                     /locus_tag="PKJ61_10890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14368.1"
                     /translation="MALTLTMALVGAGEINLETIAQAQPDLIVTAIYPTDRAGTLDST
                     QPYYGFKDLEQQEKLAAIAPIVAIKVGGMGIEVIESNTKLALALGADQAKVDADADGI
                     YLVKPEDEPETQLHKSLGLKYTELNPDGDYYWDIYSWENAGKVKSGDVILLSNEGFQQ
                     ADLLKQATFADDPALVAGQVHERRVTPLGYSSQAKSLDHIAGILSSSKDV"
     gene            complement(5722..6156)
                     /locus_tag="PKJ61_10895"
     CDS             complement(5722..6156)
                     /locus_tag="PKJ61_10895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14369.1"
                     /translation="MAVIGILVGVSSGCGPEPPRAFIAESETVLQLSAIGCSGIVKVE
                     VKDPATGEYYEGMTTLWEGSADKDAEQRSITLFESNVGYTGLPAPVPTQRVLIGFYVR
                     GSNPGGQLAGVLSAIPGGQALTVDGLVDRASVVVESCPESPN"
     gene            complement(6355..6498)
                     /locus_tag="PKJ61_10900"
     CDS             complement(6355..6498)
                     /locus_tag="PKJ61_10900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14370.1"
                     /translation="MIIFRGLMLAVILAVFGLVFLAAAKAESCALVRFGAGRGRYDWL
                     TIE"
     gene            complement(6544..6849)
                     /locus_tag="PKJ61_10905"
     CDS             complement(6544..6849)
                     /locus_tag="PKJ61_10905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14371.1"
                     /translation="MAVFGFTLLGLLVASMVFFAVMLNMAPVLRPRNGSSKDLDVARR
                     VSVVFALAGIPYVALPGGLPTDGWLRNLSYIAVPVIVAMVVRWRLDARGDDHQPGPD"
     gene            complement(7017..7388)
                     /locus_tag="PKJ61_10910"
     CDS             complement(7017..7388)
                     /locus_tag="PKJ61_10910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14372.1"
                     /translation="MVVMETLSTFDLFWTVCRVLALAGSLIVPLVFVLVGLRLTDWLG
                     YGLLAVLSVLSVLNLVDTAMWAAAIGVPAALAIAYGIARAMARAYREHPDLEFDGFFP
                     IGSPPARVRSVWPRKAAPRAK"
     gene            complement(7421..>7626)
                     /locus_tag="PKJ61_10915"
     CDS             complement(7421..>7626)
                     /locus_tag="PKJ61_10915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14373.1"
                     /translation="IRLGNLMSQMSTVISLVKDYGFVRARTIMLSKLGTAGVAAAVLY
                     NALDTIMDVLGLSSCVSLMTGKD"
BASE COUNT         1390 a         2622 c         2408 g         1106 t
ORIGIN      
        1 acgagcaggg cttcaccggg ctgaaggagg agaaccacta cggcgacgac gccgtgcggc
       61 cgccgttccc gggtgcgccg tccgagctgc tgccgttcgg ggaggccacg cacgccgact
      121 atgtcgaact catcgggatt cggcgccggc atccgtggct ggtggacgcg acggtgacca
      181 cgagcgacgt gaccaacacc tcgatcgcgg tccatctcga ctccggtgac agccacctca
      241 ccctgcggct gaacatcggc gattcggccg tggacgggct cgcgccgcac agctggtcgt
      301 tgacctgaat cgaccgatcc acgcgaccca ccgtgacgcc acggtcctgc ggatcgaaat
      361 gcgggctctc agcgcggatc gggtgctgag aaccctcatt tcgatggccg cgcgtccgtc
      421 cgctgcgcga cgagggcgcg ggcaccgtcc gtcgtggggg tcagaatcag ggtggccgag
      481 cgcgaacccc ttggcttcag gcgtttgcgc aggaccgccg ggtcgacgtc taggccgcgc
      541 accttgatct ccagaatgcc gatctggtgc tcgcgcagcc aggcgcgcag cgtccgctcg
      601 tcgttgggca gggtttcgag cacctcgaac gccgtcgcga agggcgtggc gatcagcttg
      661 tcggcgaaca gataggcgat gcccgggctc gcagcgcgtg cgtgcagggc ttccgccagg
      721 gcgccgatcg cccctgcccg gatcaccgcg gggtcgggct cgtagaggta gcctccgatc
      781 ggcccggacg ggggctcgcg gcgctcgccg gcgtccagct cggctccgga cggcaggagt
      841 accgcacgtc gacccttcct cggttcggtg agacaccaca gcgaggtctc caccaggtcg
      901 ccgcggtggc tcacccaggt ggccgcgacg ccgtccggga tcagcgtgga cggcagcccg
      961 ggcgcggcct tgatgcagcc gaaccgtccg gccagtagcg aggtcgcgaa atcccagggc
     1021 ggcgagaagt cagaaacccg ccaggtgcgt ccagacgcgg tccgccgagc cgggtcgatg
     1081 aacaccgccg cgccattggc cagcagttga ggagcgagcg tcaccgcgtc cccacagatc
     1141 acttcgccca gcccgtccag gttggcggcg gccagcacgg ccgtagccgg gtcagcctcg
     1201 accgcgatca ccttcagccc ggctgtggcg aaggccagcg cgtccgcccc aagtccgcaa
     1261 cccaggtcga ccacctcgcg caccccggcg gccacgaacc gggacgcccg ccaggccgca
     1321 acttcggccc gggttgcctg ctccaatccg tccggggtga agaacattcg ctcggcccgt
     1381 ccgccgaact tcccggcggc gcggcggcgc aattccacct ggctcgaggc agccgctgcc
     1441 tgctccgccg gccatgatcg gcgcagggct gtggctaccg ccagcgatcc cgggtcagcc
     1501 tgcctggatg cctccgccag agccggacgg gcgtccgccg acaccagcca gcgagcgagt
     1561 tcgacatcca cgacacgaac ctagcccgcc tagccatcaa tccccgcacc gcgccaatat
     1621 gatcggcgca tgaaggtgct gtcgaaactg agtctgagcc ttggcctcgt cgcgttgctc
     1681 gccgggatcg gcgtgctggt ctggggctcc tggatcgctt attggcacta tgcaaccctc
     1741 tccaccggcc gcagcgcgga gttccaggat ccgattccga tcatcgccat cgcggcgttc
     1801 gtgatcgcgg tgggcgcctt cctgaccggg ctcgcggtcg ccgcgccgcg caaggtgtat
     1861 cccgccaacc agactgtcga tccgactgtc tgacccgacg ggctagatca gccagtcctg
     1921 aatccgcagg cccagcccga ccactccggc gacggccagc aggaacaggt acgcgagcac
     1981 cagccgggtg tcgcgcaggt agcacttcga ccgggtgagt ccggcgcgtc gcgctttcca
     2041 gtagcccgcg aacccgaccc cggcaaacac caccaccgcc agcggtccga acagccagcc
     2101 aaggatggcg atagtcgcga agatgcacag ccgtagcgga tcgaacggcg ccgcgtccgc
     2161 tgtttccggc ggctcggacg catccgcgcc cccggctgcc acgaacgaag agggggacgc
     2221 cccgcgcgcg atccgagcgg cggcctgttc gggggtctcg gtcatggccg tggttccgct
     2281 cgcgcggtgc tcacccgctg gcgtccgagt cgtccccagg cctgcaccgc gcagaacggg
     2341 gcgcactgcg gatcaccgtc gtcgctgaag gtgccgacgt gcacgcagca ctgggtgagc
     2401 cgctcctcca gcatggtcga gatgtcctgg aacggcttga tcgtgatccg cttcacccgg
     2461 tcggcgagca ggtnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngagcacg
     2581 ccggtgtggg tgagccggtt catcgggtcg atgccctggc cgcgtccgga accgaacacc
     2641 ggctgaatcg ccaccccgcc aatgaacggc gtagccagcg ccttcagcac caccgcgccg
     2701 atgtcgccgt cgttgacgcc gagcgctgcg gtcatcacca gggtggtgaa caccccggcc
     2761 gccgacagcc gggcgatcgc cgcttccttg aaccgggtca ggtcggcccc gcgatgatgc
     2821 cgggacgcct ctgcggacgg cccgtcgtac tgcaggtaca cctccagccg gtcgcggtgc
     2881 gacttcagga gttccaccag cgcatcgtcg gacgcgatca gcagaccgtt cgtgttcagc
     2941 atgattcgga cgatcggacg cgccaccaac tcgtcgagca actcggccag ctgcgggtag
     3001 agggtgggct cgccgcccga cagcatcagc acgtcgagcc gtccctcctc gcgggagagt
     3061 ttcgcgtcca cgttcgccag cacgtccgcc agcggggcga ccccggtcaa cgccggcccg
     3121 gacgcggtga agcaggtcgg gcacttcagg ttgcagtgct cggtgacgtc ctggatcagg
     3181 atgcaggtgt gctgggtgtg gaaggccggc agcccgtgag cgaacgcttc gggcatcggc
     3241 cggaagttgt cggcgtggtc aggaatgtgc accttggtcg gtgcctgcca ctgctccagg
     3301 tagcgcagga tgttcgcgtg ctcgtcgtag agggtggtga tcatcccgtg cgtggggcag
     3361 gcgcgttgca gccagacccg ctcgtcgcgg acgaccagga tgccggagag ccgctgcacg
     3421 ctgctgagtt cagcgtccgg gtcgtggcac tcgggacagt aggccgtgac gtacttgtgg
     3481 atccggtcgc cgcgcaacgg ctggcccgga ccgagcgagg gacgcgtccg cggggtcacg
     3541 ttgagcagat tggtcaaggc ttgcctttcg tcagacgctg gccagcagcg cgatgcagat
     3601 gccgccgagg atcaggaccc ccgcgccgag cccgagcagg atgcccccac cggtgcgact
     3661 cgttctcggg atggccgcca gcaccagccc gcccagcagc agcacgaacg gccagtcgaa
     3721 caagagccag aagatgtcga aaccaccgct gccagagagc gcggcgactc cgaaccagat
     3781 cagcgccatc gaccccaccg cgatggccag gccgagccac agcgaacccc cgcgcggcgg
     3841 ctgtggtggc aggtggtacg gcggaccggg cggcggtgtc ggtccccacc cgggggttga
     3901 ctcactcatg gctgcctccg ttcagagcgg gtatcggagt ggcggttttg ggagtgcgtg
     3961 gccagattcg gctcgtccgc cagccgagca gcggcagggt gcagagcagg aaccattgcg
     4021 gacgggtcag gccgccccaa acctggtcgt tcgcgcggac gaattccacg ccgaaccgga
     4081 acagcgcata ggcggtgaca taggccacga acaggtcgcc ggacctcggc agccgatccc
     4141 ggtaccgcaa gagccaccag aacgcgaggc cctggaagac gagctcgtag gcgaagctcg
     4201 ggtgcaggcc gacgcctgcc gggccgtcca gcagccgggc ctgctcggga gtgagcacga
     4261 tcccccagcc gccaccggtg ggctgtccgg gcagctcggt gagcaggcag ccaacccgtc
     4321 cgatcagcat ggccagggcc accgaaggcg cgaacaggtc gccggtcgaa gcctggtacc
     4381 cggtgatcag cttgccgaca tggacgccga tccaggcgcc aacgagcccg gccagcacgc
     4441 tgcggttccc gtccatccac cactccagca ccggacggtt ctggctgagg tcgaggaact
     4501 ggaaccaggt gcccagccgg gaaccgatcg cgccgaacgc gaggcaggtg cccgcgacga
     4561 tccacagccg gtcatccttc agcccacgcc tgcgcaactc gtgggcgaac agcagcacgc
     4621 ccacgaggat gcccagcccc atgaacaggc cgtgcaccaa tccgctgcct gcgaacggaa
     4681 ggccgaccat gacgtcactc tagggggtga ttcaccgttt gggacctaac tgtggctcta
     4741 cccaccgtcc gtggtgcgct cagtcacagt caaaggcggt cgaggcgggt tacgccttcc
     4801 agaagccgga aaaggtgatc cgcgacttgt gcctgggcgc ccttcccgcg gcaaccagcg
     4861 gacatcgacc ccggccgggg ccgccacctg aactcgatgg cgctcaccct gaccatggcc
     4921 ctggtcggcg cgggcgagat caacctggag acgatcgccc aggcgcagcc cgacctgatc
     4981 gtcacggcga tctatccgac cgaccgcgcg ggcacgctgg acagcaccca gccgtactac
     5041 ggcttcaagg atctggagca gcaggagaag ctggccgcga tcgccccgat cgtcgcgatc
     5101 aaagtgggcg gcatgggcat cgaggtgatc gaaagcaaca ccaagctcgc tctcgcgctc
     5161 ggcgccgacc aggccaaggt ggacgcggac gcggacggga tctatctggt gaagcccgag
     5221 gacgagccgg agacccaact gcacaagagc ctgggcctga agtacaccga actgaacccc
     5281 gatggcgact actactggga catctacagc tgggagaacg ccggcaaggt gaagtccggt
     5341 gatgtgatcc tgctctcgaa cgagggcttc cagcaggccg acctactcaa gcaggccacg
     5401 ttcgccgacg acccggccct ggtcgcgggc caggtgcatg agcgccgggt caccccgctg
     5461 ggctactcct cccaggccaa gagcctcgat cacatcgccg gcatcctaag ttcctcgaag
     5521 gacgtgtagt cggaagcgtg atctcgacag gctcgatcag ccgaacggtc gcgcctgtcg
     5581 agaccgcacg ctctctccag atcggcccct gaggaacgag cgccagcgag tgtcatccct
     5641 ccacagcggt ccctgaggaa cgagcgccag cgagtgtcac gaagggccga acccccggcc
     5701 cgggaacagc ttgagggggc tctagtttgg agactccggg caagactcca cgaccacgga
     5761 cgcacgatcc accagcccgt ccacggtcag ggcctgccca ccgggaattg cactgagcac
     5821 tcccgcaagc tgaccgcccg ggttggatcc gcgcacatag aagccgatca gaacccgttg
     5881 agtcggcacg ggtgccggca gtccggtata gccaacgttc gattcgaaca gtgtaatgct
     5941 gcgttgttcc gcatccttgt cggcggaccc ctcccagagt gtggtcatgc cctcgtagta
     6001 ctcgccggtg gcagggtcct tcacctccac cttcacgatg ccagagcagc cgatcgcact
     6061 gagctgaagc accgtctcgc tctcggcgat gaatgcgcgt ggaggttcag gcccacaacc
     6121 agagctcacg ccgaccaata tgccgatcac cgccacggcc catagtctga gcaaggcgtg
     6181 ccgcgcgatc cgcgccctca ttggaggctt cagcacgtgt agctgccctt cttgagatca
     6241 gccatgatct cgaagatgtt gccacagccg gaagcgccgg tcgatgagtt gcgatgaaac
     6301 accagccccc acgacgatgc gatcaggccc gcagtcagac ggttacgacg ccggtcattc
     6361 gatggtgagc cagtcatagc gccctctccc ggccccgaac cggacgagcg cacaagactc
     6421 ggccttggcg gccgcgagga acaccagtcc gaacaccgcg aggatcacgg ccagcatgag
     6481 cccgcggaag atgatcacaa agcctccgac tccaacagct ggcatgctag gtcaacttct
     6541 cgctcaatcg ggtccgggct ggtggtcatc cccgcgagca tcgagtctcc atcgaaccac
     6601 cattgcgacg atcaccggca ctgcgatgta cgagaggttg cgaagccaac catcggtcgg
     6661 cagtccgccc gggagcgcaa catagggaat tcccgccagc gcgaagacaa ccgacactcg
     6721 ccgcgcgaca tcaaggtctt tgctcgagcc gtttctcgga cgaagtaccg gcgccatgtt
     6781 gagcataacc gcgaaaaaca ccatcgacgc caccagcagt cccagcagcg tgaatccgaa
     6841 gactgccatc ctgttaaccc cgcagaatcc tcttgaaaat cccccaacat gtgtcgatcc
     6901 caatcacgac catgagcgcc tcaccacccc gccggaagat ctcctcgacg gccgccgcct
     6961 gggccttgct tagtgccaca gccggcagcg ctggtcgatg ggttgcgatc accacctcac
     7021 ttcgctcgcg gtgcagcctt cctgggccag accgaccgga cgcgcgctgg cgggctgccg
     7081 ataggaaaga agccgtcgaa ctccaagtcc gggtgctccc gatacgcccg ggccatggcg
     7141 cgcgcaatgc cgtacgcgat ggccaaagcg gccggaactc cgatcgccgc agcccacata
     7201 gcggtgtcca ccagattgag cacgctcagc acggacaaca ccgcgagcaa gccatagccc
     7261 aaccagtcgg tcagccggag cccaaccaac acgaacacca acggcacaat caagctgcca
     7321 gccagagcca gcacgcggca aaccgtccag aacaggtcga aagtcgacaa agtctccatg
     7381 actaccatgc tgcctcctca ggccatagca gtgactcttc ctagtccttc ccagtcatca
     7441 ggctcacgca cgagctcaac cccagaacat ccatgattgt gtcgagcgcg ttgtagagca
     7501 cggccgcagc caccccggcg gttcctagct tggagagcat aatggtccga gccctgacga
     7561 atccgtaatc cttgacgagc gatatcaccg ttgacatctg tgacatcaag ttaccgagcc
     7621 taatcc
//
LOCUS       DAOPRQ010000099         7492 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_8172_length_7492_cov_11.061987, whole genome shotgun sequence.
ACCESSION   DAOPRQ010000099 DAOPRQ010000000
VERSION     DAOPRQ010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 7492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7492
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8172_length_7492_cov_11.061987"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="PKJ61_10920"
     CDS             complement(<1..516)
                     /locus_tag="PKJ61_10920"
                     /inference="COORDINATES: protein motif:HMM:NF015237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HOC14374.1"
                     /translation="MSRSRSASTTTPKPRGRLRRLLDRIPIPRPDTKRGAIFLILAVL
                     AVGAAVTLAGVHVVEYSGSTAFCTQLCHTMEPQKKAHAASTHSEVECGSCHVAPGVYG
                     FVKAKLEGTHELFALITNTYPRPIPAIEHSKLPSTTVTCQACHPLATRRPASTSRTSA
                     SPRSGSRTTPAT"
     gene            complement(562..1272)
                     /locus_tag="PKJ61_10925"
     CDS             complement(562..1272)
                     /locus_tag="PKJ61_10925"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOC14375.1"
                     /translation="MSDHVDESFDEFLDDDVDEPARPVRLAPVDVLSDAPAGARERIR
                     SSKLGTLVVLLITAAVVAGGVWLVNQGNAKNAAAQTAGGTVVKMPGISKVAPPEVGKP
                     AQDFTITTYDGKSVSLSGLKGKAVWVTFGASWCSGCQAEVPDINAAAKAFESKGVVVL
                     GVNISEDNAAVKAYAQRIGLMYPIGADPESAIADDYAVSAIPSHFFIDANGVVRDIRL
                     GALSPSMMESILNKLVAS"
     gene            complement(1324..2283)
                     /gene="ccsB"
                     /locus_tag="PKJ61_10930"
     CDS             complement(1324..2283)
                     /gene="ccsB"
                     /locus_tag="PKJ61_10930"
                     /inference="COORDINATES: protein motif:HMM:TIGR03144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HOC14376.1"
                     /translation="MLVYSQYAIVAALVATALASICYIWAVVRGHQEIMAASGVQRPT
                     KVLVGTTGDHLDDLDSLTETPVASTQVAPSGPAAGSLARYGSLLAWLALGFLTVALVL
                     RTIVTGHGPFVTQHEFATSTAWGFLAMYLFFEWRYRARTLALLMLPITAGALLYALSW
                     ATTADPLVPALQNSLLLTTHIIVAVFAYGAFAVSAAAAGLYLLVSYWPERFGHVLPKE
                     DVLDRLGYRAVVIGYPLLTMVIVLGAVWAEIAWGSYWSWDPKETASLVTWLIYGGYLH
                     ARVARGWVGRGAAYLLLVAFASVILTFVGNAFFGGLHSYGTGR"
     gene            complement(2319..3788)
                     /locus_tag="PKJ61_10935"
     CDS             complement(2319..3788)
                     /locus_tag="PKJ61_10935"
                     /inference="COORDINATES: protein motif:HMM:NF016995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HOC14377.1"
                     /translation="MTTPVKDGAEVTGSVPGHVDGVDVLDGGRRPSAEGVSILGRVWG
                     FFISMRIGLFIILILGLLTLIGTVIGQVSEEVLADPVAYQQWFEAGPRQKFGGWATVF
                     DALGLFHVFSAWYFRALFAILAVSILACSVNRAPRLWRVATKPRTSMNDAFFAAAPLK
                     AEFVLPVGVDAAVERVKAGFAQEHFRVLDGSKKSKGADVYADKFRWGPFGTVVAHLSF
                     LIILAGFVVSGTSGFKDNQVIAPIGVPVAVGHDTGLTVTAMSFQDSYYDNGSPKDYVS
                     DLVVTKGGVQVARQETRVNTPLIVDGVWFHQSFFGVGADVTATKDGQTIFTDTVPLQW
                     TSNDGQRSIGQFAIQSAGVSVYVIQAASGAKLADLPAGSVALEVHKTGVTDPQVQVVS
                     QGKSATIDGITYTFDRNRQYTGLAITRDPGSWIVWLGSILLILGSYLTFFLPHRRVWV
                     RVRPTAKGSSIQVGSPIKRDPAFEPRFNDIVARIQSTES"
     gene            complement(3812..4546)
                     /locus_tag="PKJ61_10940"
     CDS             complement(3812..4546)
                     /locus_tag="PKJ61_10940"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HOC14378.1"
                     /translation="MIEVGLAVAFIAGVASFASPCCLPMVPVWVSYVVGANPTPGRAA
                     RSVALRQSLAFVAGFTVVFVALWASLGLVGYALRDQAALLRQIGGAVLIIMGLHVAGL
                     ISIPVLGRSMGLDAGRLVKRRADGTVAQQGPSLGRSLLFGVVFAAGWTPCVGPTLGAI
                     IGMASLRSSFAQGTLLLLVFALGLGLPFVLVALGADAVRRRLAWFTAHEAVVSVVTGV
                     LLMIVGFLMITNLLVRLSQFFPQFPV"
     gene            complement(4551..5399)
                     /locus_tag="PKJ61_10945"
     CDS             complement(4551..5399)
                     /locus_tag="PKJ61_10945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14379.1"
                     /translation="MTADTTSTGTSSVRQDLLARLGLPVDAGAEDVSATHGQIVEFLD
                     TAPPAITGWAKRRRKEVDKIESLLSGPESALQAMARPVVAPARASLPKPLLVLLGLVL
                     LAGIIVGVYWLGRPTPNVPSMTSAETPAATPTVDAAALADLMKKVEANPKDVASLTSI
                     AALYFNAADFKNSATFQQKVLDIEPTNEQALIDAGACAFYQSDMTNAEKYWAAGVAAH
                     PNNPELRYDLGFIYMTTGRSAQMKEQWDKVVELAPNSTLAKTVQQQVGSIKSTASPAP
                     SATPSK"
     gene            5773..6045
                     /locus_tag="PKJ61_10950"
     CDS             5773..6045
                     /locus_tag="PKJ61_10950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14380.1"
                     /translation="MGEGIAARLRDRGVATTVLARPDGATMSGELAKYPSVIIAEWAT
                     PECPALVGQLSPTSRIVDVGTVIGHGFSDSADLAHFSAILDALASA"
     gene            complement(6053..7258)
                     /locus_tag="PKJ61_10955"
     CDS             complement(6053..7258)
                     /locus_tag="PKJ61_10955"
                     /inference="COORDINATES: protein motif:HMM:NF024905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HOC14381.1"
                     /translation="MLSGAAQMFERGGRLDARRAVVFARARLRQGIRRVRTALEPVLI
                     AAVGAALAWAIAHDALHQPAPLFAPMAAWICLGFTKNRSPRIVAELALGATVGVGLGE
                     LFVSTFGAGAWQIGVVLVVAALAGRLLDRGDVVTFQTVGNGLVIVGLSAIPGMELHAG
                     GRWMDALVGGAVAFVLTVALPRSVVERPRRYARSAFAEMAQAIEMLADGLRRGDVEAM
                     MDVEGQVRATTESIDDFDKALKAAADVAALNPTLRAEVPQLDELKRILALARRAETGL
                     AMMVRQAVGFYEQAGRLPDVGDRLAEVGPAVRALSTSIGGWHRPQHARDVLSDVAAKT
                     APHEIESQDWRPAALMSLVRALVSDLLQVTGLSRSDAAARLAATHGAPFAAEREASPE
                     DEASALWGD"
     gene            complement(7260..>7492)
                     /locus_tag="PKJ61_10960"
     CDS             complement(7260..>7492)
                     /locus_tag="PKJ61_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HOC14382.1"
                     /translation="RPADKPLLDIFATIVVERDSQKGIIIGHQGERLREVGRDARLQI
                     NKLLGTPTHLALKVKVMRDWQRDAKQLNRLGF"
BASE COUNT         1276 a         2492 c         2640 g         1084 t
ORIGIN      
        1 cgttgccggc gtcgttcgac tgccggatct tggcgaggct gatgtacgtg acgtgctcgc
       61 cggtcttcgg gtcgccagcg ggtggcaggc ctggcaagtc accgtggtcg aaggcagctt
      121 cgagtgctcg atcgcgggga tcggccgcgg gtaggtgttg gtgatcaggg caaacagctc
      181 gtgggtgccc tccagcttcg ccttgacgaa gccgtagacg ccgggggcca cgtggcagga
      241 cccgcactcg acctcggagt gagtggacgc cgcatgcgcc ttcttctgcg gctccatcgt
      301 gtggcagagc tgggtgcaga aggccgtgct cccggagtac tcgaccacgt gcaccccggc
      361 cagggtcacg gcagcaccca ccgccaggac ggccaggatc aggaagatcg cgccgcgctt
      421 cgtgtcggga cgcggaatcg ggatgcggtc cagcagccgg cggaggcgtc cacggggctt
      481 gggggtggtg gtggacgccg aacggctccg cgacatcgtt gtcggagacg tcaggggcat
      541 ccggggagtc gggatcatcg gtcatgacgc caccagcttg ttgaggatgg attccatcat
      601 cgacggcgac agggcgccga ggcggatgtc gcggacgacg ccgttggcgt cgatgaagaa
      661 gtgcgaggga atcgcggaga ccgcgtagtc gtccgcgatg gcggactctg ggtcggcgcc
      721 gatcgggtac atcagcccga ttcgctgggc gtacgccttc acggcggcgt tgtcctcgga
      781 gatgttgacc cccaagacga caacgccctt gctttcgaac gccttggctg cggcgttgat
      841 gtcggggacc tcggcctggc agccggagca ccagctggcg ccaaacgtca cccagacggc
      901 cttgcccttg agtcccgaca gcgacaccga cttgccgtca taggtcgtaa tggtgaagtc
      961 ctgcgccggc ttgcccactt ccggaggggc caccttggag atccccggca tcttgacgac
     1021 ggtcccgccg gcggtctgcg cggcagcgtt cttggcattg ccctggttga ccagccacac
     1081 gcccccggcg accaccgcgg cggtgatcag caggaccacc agggtgccga gcttggacga
     1141 cctgatgcgc tcccgggccc cagcaggagc gtccgacagc acatccaccg gcgccagccg
     1201 gacaggacgg gccggctcat cgacgtcgtc gtcgaggaac tcgtcgaacg actcgtccac
     1261 gtggtctgac atgttcgttc ccttcccgtg ccgggccacc ggggcccggc gtgaggcgtc
     1321 cggctagcga ccggtgccgt aactgtgcag gccgccgaag aaggcgttgc cgacgaaggt
     1381 caggatgacc gaggcaaagg cgacgagcag caggtaggcg gcgccacgtc cgacccagcc
     1441 gcgagcgacg cgtgcgtgca ggtagccgcc gtagatgagc cacgtgacca gggacgccgt
     1501 ctccttggga tcccagctcc agtagctgcc ccacgcgatc tcggcccaga cggcgcccag
     1561 gacgatcacc atggtgagca gggggtagcc gatgacgacc gcgcggtagc cgagccggtc
     1621 gaggacgtcc tccttgggca ggacgtgacc gaagcgctcc ggccagtacg agacgagcag
     1681 gtagaggccc gcggcggcgg cggagaccgc gaaggcgccg taggcgaaca cggcgacgat
     1741 gatgtgcgtg gtcagcagca ggctgttctg cagggccgga accagcggat cggccgtcgt
     1801 ggcccacgac agcgcataca gcagggcgcc ggccgtgatg ggcaacatca gcagggcgag
     1861 ggtgcgggcc cggtaccgcc attcaaagaa gaggtacatc gcgaggaagc cccacgcggt
     1921 ggacgtcgcg aactcatgct gggtgacgaa cggtccgtgg ccggtgacga tcgtgcgcag
     1981 caccagggcg acggtgagga acccgagggc gagccaagcg agcaacgacc cgtaccgagc
     2041 caggctgcca gcggcgggtc cggacggggc cacctgcgtg ctggccaccg gggtctcggt
     2101 cagggaatca agatcatcga ggtgatctcc ggtggtgcct accagcacct tggtggggcg
     2161 ctgcactccg gacgccgcca tgatctcttg gtggcccctg acgacggccc agatgtaaca
     2221 gatgctggcc agggcggtgg cgaccagcgc cgcgacgatc gcgtactggg agtagacaag
     2281 catggcggtt gctccttcgc ttgacggtgg ctaggtggct aggactcggt ggactgaatg
     2341 cgggcgacga tgtcgttgaa ccgcggctcg aaggccgggt ccctcttgat cggcgagccg
     2401 acctggatgc tgctgccctt cgcggtgggg cggacccgca cccagacgcg gcggtgggga
     2461 aggaagaacg tgaggtagct gcccaggatc agcaggatgc tgcccagcca gacgatccac
     2521 gatccggggt cacgcgtgat ggcgaggcct gtgtactggc gattccggtc gaacgtgtag
     2581 gtgatgccgt cgatggtggc cgacttgccc tgactgacca cctgcacctg gggatcggtg
     2641 acgccggtct tgtgcacctc gagggccact gacccggccg gcaggtccgc gagcttcgcc
     2701 ccggacgccg cctggatgac gtagacggac acaccggccg actggatggc gaactgccca
     2761 atcgaccgct ggccgtcgtt ggaggtccac tgaagcggaa cggtgtcggt gaagatggtc
     2821 tgaccgtcct tcgtcgccgt gacgtcagcg cccaccccga agaacgactg atggaaccaa
     2881 acgccgtcca cgatcagcgg cgtgttgacc cgggtctcct gccgggccac ttggacgccc
     2941 cccttggtca cgacgagatc ggacacgtag tccttcggcg agccgttgtc gtagtagctg
     3001 tcctggaacg acatcgccgt cacggtcagc ccggtgtcgt gccccacggc cacggggacg
     3061 ccgatcggcg cgatgacctg gttgtctttg aaaccggagg tcccggagac gacgaacccg
     3121 gccaggatga tcaagaagct gaggtgggcc accaccgtgc cgaaggggcc ccagcggaac
     3181 ttgtccgcgt acacatccgc acccttggac ttcttactgc cgtcgagcac ccggaagtgc
     3241 tcctgagcga agccggcctt gacccgctcc accgcagcat cgacgcccac cggcaggacg
     3301 aactcggcct tcagcggcgc cgcagcgaag aacgcgtcgt tcatcgacgt gcggggcttg
     3361 gtggccaccc gccacaggcg aggggcgcgg ttcaccgagc aggcgaggat gctcacggcc
     3421 aagatggcga acagtgcccg gaaataccag gcggagaaca cgtgaaagag gccgagcgcg
     3481 tcaaagaccg tggcccagcc gccgaacttc tgcctcggac ccgcctcaaa ccactgctgg
     3541 taggccacgg ggtccgcgag cacctcctcg gacacctgac cgatgaccgt gccgatgagg
     3601 gtgagcaggc cgaggatcag gatgatgaac aggcctatcc gcatcgagat gaagaagccc
     3661 cacacccggc cgaggatgct cacgccctcg gcgctcggac gccgcccacc gtcgaggacg
     3721 tcgacaccgt cgacatgccc cggaacgcta ccggtgactt cggcgccatc cttcacaggt
     3781 gtggtcatca cgcaactact ttcagacggt ttcagacggg gaactgcggg aagaactggg
     3841 agagcctgac gagcaggttg gtgatcatca ggaagccgac gatcatcagc aggacgccgg
     3901 tcacgacgga gacgacggcc tcgtgcgcgg tgaaccaggc gagacgacgt cggacggcgt
     3961 ccgcccccag tgccacgagc acgaagggca ggccgagccc gagcgcgaag accagcagca
     4021 gcagggtgcc ctgggcgaag ctggaccgaa gggacgccat cccgatgatc gcgcccagtg
     4081 ttgggccgac gcacggcgtc cagcccgccg cgaataccac gccgaacagc agcgagcggc
     4141 cgaggctcgg cccctgctgc gccaccgtgc cgtccgcgcg gcgcttgacg aggcgtccgg
     4201 cgtcgaggcc catcgagcgg cccaggaccg ggatggagat gagccctgcg acgtgcaagc
     4261 ccatgatgat caggaccgct cccccgatct ggcgaagcag cgccgcctgg tcgcgcaggg
     4321 cgtagccgac gaggccgagc gaggcccaga gtgcaacgaa gacgaccgtg aagccggcga
     4381 cgaaggccag ggactgccgc aacgcgaccg agcgagcggc gcgacccggg gtggggttgg
     4441 caccgacgac gtagctcacc cagaccggga ccatgggcag gcagcagggc gacgcgaacg
     4501 acgccacccc cgcgatgaac gcgactgcca gcccgacctc gatcatgggg tcacttgctc
     4561 ggcgtcgctg atggagcggg cgaagcggtg gacttgatgg atccgacctg ctgctggacg
     4621 gtcttggcca gcgtggagtt cggggccagc tcgacgacct tgtcccactg ctccttcatc
     4681 tgcgcgctgc ggcccgtcgt catgtagatg aagcccaagt cgtagcgcag ctcggggttg
     4741 ttcggatgcg cggccacgcc agcggcccag tacttttcgg cgttcgtcat gtcgctctgg
     4801 tagaaagcac aggccccagc gtcgatgagc gcctgctcat tggtcggctc gatgtcgagg
     4861 accttctgct ggaaggtcgc agagttcttg aagtcagcgg cattgaagta cagcgcggca
     4921 atcgaggtca gcgacgccac atccttcggg ttcgcctcga ccttcttcat caggtcggcc
     4981 agggcggcgg cgtccaccgt gggggtcgcg gcgggcgtct cggcagacgt catcgagggg
     5041 acgttcggcg tcggccgtcc gagccagtac accccgacga tgatccccgc cagcaagacc
     5101 aggcccagca ggacgagcag cggcttgggc agcgaggccc gggctggcgc gaccaccgga
     5161 cgcgccatcg cttggagcgc cgactccggc ccactcagca acgactcgat cttgtccact
     5221 tccttgcggc gacgcttggc ccagccggtg atcgccggcg gggcggtgtc gaggaactcg
     5281 acgatctggc cgtgggtggc ggacacgtcc tcggcgccgg cgtccacggg aagcccgagt
     5341 ctggccaaga ggtcctggcg gacactggag gtgccagtgg acgtcgtatc ggcagtcatg
     5401 gaaacggttg ctccctcaag ctcacatcac agcggtggaa tgttcgactt aagtgtcgtt
     5461 ttgagcgatt tgtaacgcta tgtcccgaag ggcacatatc aggtagaact tgacgtcgga
     5521 tccggccatc gcggccgagg tcgacgccct cgccgaggtg tgcgccgacg cctacgagga
     5581 cgcccgcgag atgatcctcg cgctgcgcga cgccgatcga tccgaccgcg gcctggccga
     5641 ggggctgcgc gacttcctcg ggaagggcag cggacgagtt tcccccagga aggcccccac
     5701 ctggtcagca gcgcaacgag cggcgtcctc gccccgggcg cgacggcact ggtcgtctac
     5761 gagcatgagc tgttgggtga agggatcgcg gcccgcctcc gggatcgcgg ggtcgcaacc
     5821 accgtcttgg cgcgtccgga cggcgcgacg atgtccggcg agctggcgaa gtatccgtcg
     5881 gtgatcatcg ccgagtgggc gacccccgag tgcccggcac tggtgggtca gctcagcccg
     5941 acgtcacgca tcgtcgacgt cggcaccgtc atcggacacg gcttctccga ctcggcggat
     6001 ctcgcgcact tcagcgccat cttggacgcc ctcgcctcgg cctgagccgg gctcagtccc
     6061 cccacagcgc ggacgcctcg tcctcggggc tggcctcgcg ctcggccgcg aagggggcgc
     6121 cgtgcgtggc ggcgagccgc gcggcggcgt ccgacctcga tagcccggtg acctggagga
     6181 ggtcggagac cagggcgcgc acgagcgaca tcagggccgc cggacgccag tcctgagact
     6241 cgatctcgtg gggggccgtc ttggccgcta cgtccgacaa gacgtcgcgg gcgtgctggg
     6301 gacggtgcca gccgccgatc gacgtcgaca gggcgcgcac cgccgggccg acctcggcga
     6361 gccggtcccc cacgtcgggg aggcgtccgg cctgctcgta gaacccgacc gcctggcgca
     6421 ccatcatcgc cagcccggtc tcggcgcggc gtgccagcgc gaggatgcgc ttcagctcgt
     6481 cgagctgggg aacttcggcg cgcagcgtcg ggttcagcgc cgcgacgtca gcggccgcct
     6541 tgagcgcctt gtcgaagtcg tcgatggact ccgtggtcgc ccggacctgg ccctcgacgt
     6601 ccatcatcgc ctcgacgtcg ccccgcctca gcccgtcggc cagcatctcg atggcctggg
     6661 ccatctcggc gaaggccgaa cgggcgtaac ggcgcggacg ctccaccacc gagcgcggca
     6721 gcgccaccgt gagcacgaag gcgacggcac cgccgacgag ggcgtccatc cagcggccac
     6781 cggcgtgcag ttccatcccc ggaatggcgg acaggcccac gatgaccagt ccgttgccga
     6841 cggtctggaa cgtgacgacg tcgccgcggt cgaggaggcg tccggctaag gccgccacga
     6901 ccagtacgac cccgatctgc caggcgccgg cgccgaacgt cgagacgaag agttcaccga
     6961 ggcccacgcc gacggtggcc cccagcgcca actcggccac gatgcggggc gagcggttct
     7021 tggtgaagcc gaggcagatc caggccgcca tcggcgcgaa caggggcgcc ggctggtgga
     7081 gcgcatcgtg cgcgatcgcc cacgcgagcg ccgcgccgac cgcggcgatg agcaccggct
     7141 ccagcgccgt ccgaacccgc cggatgccct ggcgtagccg agcgcgggcg aagacgaccg
     7201 cgcgacgggc gtccaaccga ccgccccgct cgaacatctg ggcggcaccg ctcaacacgt
     7261 cagaacccga ggcggttcag ctgtttggcg tcccgctgcc agtcgcgcat gaccttgacc
     7321 ttgagggcga ggtgggtcgg ggtgcccagg agcttgttga tctggagccg cgcatcgcgc
     7381 ccgacctccc gcaggcgctc gccctggtgg ccgatgatga tgcccttctg cgaatcccgt
     7441 tcgaccacga tggtcgcgaa gatgtcgagc agcggcttgt ccgccggacg cc
//
LOCUS       DAOPRQ010000100         7207 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_8586_length_7207_cov_13.716163, whole genome shotgun sequence.
ACCESSION   DAOPRQ010000100 DAOPRQ010000000
VERSION     DAOPRQ010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 7207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7207
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8586_length_7207_cov_13.716163"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..355)
                     /locus_tag="PKJ61_10965"
     CDS             complement(5..355)
                     /locus_tag="PKJ61_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002516921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOC14383.1"
                     /translation="MTETPWPTEWLRGVLEVCVLAVIDRGPTYGYAISAELAAAGLGD
                     LKGGTLYPLLGRCEQAGWVAVEWRVGESGPGRKYYTLTPSGRTELRGRASRWHEFVDV
                     TGSLLAASELKEKS"
     gene            634..1992
                     /locus_tag="PKJ61_10970"
     CDS             634..1992
                     /locus_tag="PKJ61_10970"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOC14384.1"
                     /translation="MPARRQLTIAEFTDNYGPAHSGLLYAVQFLEEQVLAAGHKLLLV
                     APEANGPNPHAGHPARREIRLPSVPIPGTHIRLSMGQDFDYRLAQMVANPPDVIHVHG
                     LGAIGLLGMWVAERTGKPLVITWHTDLEAYAEHYWHVVPFLNAAYKVYEMHMAESTWE
                     QIKKLKFKRPRRGGAQVELLQLASKMLTDADLVTTPSDKTAKRVLEIAPAAQVRVVPN
                     GTDALPELPPIAKGRGPRLIYVGRISLEKGIGLMLDAFELVRGQIPNVDLMIVGDWKS
                     TPPKLRRRLQRAARFGGVKLPGQIPREKLGAYYASGDAFVFASLTDTQALVLHEAAHA
                     GLPFVMVDHELRLVVEPGVNALLARPNAVSLAGAMVSMLNNLKDPEFAESARARSKEL
                     AAKWTIAHQSEEFVRIYEALADGREVELTEGLDPQIGRRVFPRRKVTAQFEPKELDSA
                     AE"
     gene            complement(1946..3055)
                     /locus_tag="PKJ61_10975"
     CDS             complement(1946..3055)
                     /locus_tag="PKJ61_10975"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOC14385.1"
                     /translation="MIVLIPSYEPDERLTNLVAELGHVPDLKVLVVDDGSGADYAGIF
                     ADAARAGAEVLVHEVNRGKGVALRTGFGYIRNLYPDEPVVCADADGQHTVLDILRVAA
                     AVRDSGELVLGVRRFTGRVPLRSRLGNRITAAVFTVLTGTRLPDTQTGLRGYPAGLLD
                     WASAVPGVRFEYELNLLLRATRDRLPIRQIEIATVYHADNASSHFRPVRDSLRIYRPL
                     LAYAASSLAGFVVDATVLFTWYAITGNLLTAVVVARLLSATVNFLVNRHAVFEADDVP
                     IWPSVRRYALLAAVVLALNAALMAVLTPVLGVVAAKLVTELGLFVGGFMVQHRLVFAH
                     GRATNPPELDQPVELSRPVELTRPRSPTPLARTAR"
     gene            complement(3059..4039)
                     /locus_tag="PKJ61_10980"
     CDS             complement(3059..4039)
                     /locus_tag="PKJ61_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HOC14386.1"
                     /translation="MMISTEPLPDVQADAQGPTRRPFARRTFLVAGGGTLLAAAGGTG
                     WALNRYVIEHVEVTDASAQQPANATVAQPASGTATADAYTSDTASIKISKRTSGSGNS
                     LLTWFVADITVTDATIVRSAFANDSFGENIVANPSVIAAQTGAMLAINGDYYGFRDTG
                     IVIRNGVAYRDQGARQGMAIRTDGSMSLYDETKTNAAELLADGVWQTLSFGPGLVENG
                     EVIAGIDNLEIDTNFGNHSVQGNQPRTGVGVIASNHFLFIVVDGRSTGYSRGASMSEF
                     AQMFVDEGAKVAYNLDGGGSSAMVFNGSLVNNPLGRGRERGTSDILYVAG"
     assembly_gap    4273..4372
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4373..5826)
                     /locus_tag="PKJ61_10985"
     CDS             complement(<4373..5826)
                     /locus_tag="PKJ61_10985"
                     /inference="COORDINATES: protein motif:HMM:NF025620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding domain-containing protein"
                     /protein_id="HOC14387.1"
                     /translation="MTITPIRKLTAGIVALATGLVFTACTSTTTTSSTTTGTTQTTTQ
                     TASGSTTMVASAAAALADNAESHADANDADYDASDAVTITLADGASKASSSDGVKVDG
                     DVITISAPGTYVLTGSLSNGQVVVNSEAEGKVKLVLDNAAIVSTSGSAVVITAADEAV
                     VILADGSTNTLVDGTGYDTSADDAPNAALFSMADLTIGGTGALTVTGNTNDGIASKDG
                     LVILSGTITVSAKDDGIRGKDYLIIADGTVNVTAEGDGLKSDNEDDDTVGYVSIDGGT
                     VEIAAGDDGIHAEGDLAVNSGKLTVTRSNEAIEGATISIAGGEVDVTASDDGLNATSG
                     TSTDTGGRGGGGMQDDGSLLTISGGTLLVNADGDGLDSNGALVITGGTTVVSGPTNSG
                     NGALDSNGGITVTGGTVLAAGSAGMAETPGTESTQGWVAVTLDQAVAAGQTISIVTDG
                     TVIASYTAVKQVQSVVLSDPDLKTGESYDVYVGG"
     gene            complement(5841..6650)
                     /locus_tag="PKJ61_10990"
     CDS             complement(5841..6650)
                     /locus_tag="PKJ61_10990"
                     /inference="COORDINATES: protein motif:HMM:NF020916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HOC14388.1"
                     /translation="MTALAYAEPVAAVRPRSLDELPTITLEQLLSQAELQTRIDRKYL
                     VEADAVPTLLGQADADLRVLSIAGRRRFAYRSTYFDTPELDAFLLAGRGRRRRFKVRT
                     RVYRDSGEAWLEVKTRGPRGTTVKDRMPYDLADAGRLTTVGLDFIAATLAAQEVFGVD
                     VAALQPTLHTGYDRSTLLVSSRTSDQVSRATIDSNLVWRRPGSEHSLATPGSVIIETK
                     GGATASAIDRALWYSGVRPSCVSKYGAGLAALGEDLPELKWRRALNQLNPE"
     gene            complement(6647..>7207)
                     /locus_tag="PKJ61_10995"
     CDS             complement(6647..>7207)
                     /locus_tag="PKJ61_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016460634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HOC14389.1"
                     /translation="LIAADLVAIVVLVFGLFLRRHQRRDLIVAFLVVNVGVLALMATL
                     SSTTLSVGVGLGLFGVLSIIRLRSEELRQHEVAYYFAALTLGLLGGSTGLDLVWVLGL
                     MTAVVATLYLGDHPAIAKRTERQQIQLDRAYTDEVALRARVESLLGGNVDAITVVKTD
                     LVNDTTLVDVRFVRAAKPAPVAAVAR"
BASE COUNT         1133 a         2363 c         2475 g         1136 t
ORIGIN      
        1 tcgctcatga tttctccttc agctcggacg cggccagcag cgagccggtc acgtcaacga
       61 attcgtgcca ccggctggcc cggccacgca attccgtccg cccggacggg gtgagcgtgt
      121 agtacttgcg cccgggaccg ctctccccca cgcgccattc gacggcaacc cagccggcct
      181 gctcacagcg tccgaggagg gggtagagcg tgccgccctt caaatcgccg agcccagcgg
      241 cggccagttc cgccgagatg gcatagccgt aagtcggacc ccggtcgatc accgccagca
      301 cacagacctc gagcactccg cggagccact cggtgggcca cggcgtctcg gtcatggcta
      361 gatagtaggg ctatctagtt agtgtcacaa cctagctggc gaagttgtag gccaaagtgc
      421 ggccgtccgc tggagaacgc cagcgatccg gacagttgcc ggcgaacatt ccccggcgaa
      481 tgtcagtttc gctggcgtgt tcgcaccagg caaacctgca cgcgcgggga acttcggacg
      541 ggttcgcgtc cggccgctag attaggcacg gaaacccgcg aggaaggccc tgagatcagc
      601 gacgacagcc ccgccaccgc aggcgccggc gcagtgccgg cccgccgaca gctcacgatc
      661 gccgagttca ccgacaacta cggcccggcg cattcgggtc tgctgtacgc cgtccagttc
      721 ctcgaagagc aggtgctggc ggccggccac aagctgctgc tggtcgcgcc cgaggcgaac
      781 ggtcccaacc cgcacgccgg ccacccggcc cggcgcgaga tccggctgcc cagcgtcccg
      841 attccgggga cgcacatccg cctctcgatg ggccaggact tcgactaccg cctcgcccag
      901 atggtcgcca acccgccgga cgtgattcac gtccacggcc tcggcgcgat cgggctgctg
      961 ggcatgtggg tggccgagcg caccggcaag ccgctggtga tcacctggca caccgacctg
     1021 gaggcctacg ccgagcacta ctggcacgtg gtgccgttcc tgaacgcggc ctacaaggtg
     1081 tacgagatgc acatggccga gagcacctgg gaacagatca agaagctcaa gttcaagcgt
     1141 ccccgccgcg gcggcgccca agtcgagttg ctgcagctcg cgtccaagat gctcaccgac
     1201 gccgatctgg tcaccacccc gtccgacaag acggcgaaac gcgtgctcga gatcgccccg
     1261 gccgcgcagg tgcgcgtagt acccaacggc acggacgcgc tgccggagct gccaccgatc
     1321 gcgaagggac gcggcccgcg gctgatctat gtcggccgga tctcgctcga aaagggcatc
     1381 gggctgatgc tggacgcgtt cgagctggtt cgtggccaga ttccgaacgt ggacctgatg
     1441 atcgtcggcg actggaagag caccccaccc aagctgcgcc ggcgcctaca gcgggccgcc
     1501 cggttcggcg gggtgaagct gcccggacag atcccgcggg agaagctcgg cgcctactac
     1561 gcctccgggg acgcgttcgt gttcgcctcg ctcaccgaca cccaggcact cgtgctgcac
     1621 gaagccgcgc acgcagggct gccgttcgtg atggtcgacc acgagctgcg gctggtggtc
     1681 gaacctgggg tgaatgcgtt gctggcaagg ccgaacgcgg tctcgctagc cggcgcgatg
     1741 gtgagcatgc tgaacaatct caaggacccg gagttcgccg agtccgcccg cgcccggtcg
     1801 aaggaactgg ccgcgaagtg gacgatcgcg caccagtccg aggagttcgt ccgcatctac
     1861 gaagcgctcg cggacggccg cgaggtcgaa ctcaccgagg gtctggatcc gcagatcgga
     1921 cgcagggtct tcccgcggcg caaggtcacc gcgcagttcg agccaaagga gttggactcc
     1981 gcggccgagt gagctcaacc ggccgactca gctcaacggg ctggtcgagt tccggcgggt
     2041 tggtggcgcg gccgtgcgcg aagactaggc ggtgctgaac catgaagccg ccgacgaaga
     2101 ggccgagttc ggtgacaagc ttggccgcga ccacgccaag caccggcgtg agcacggcca
     2161 tcagcgccgc gttcaaggcc agcaccaccg cggccagcag cgcgtagcgt cgcacggatg
     2221 gccagatcgg cacgtcgtcc gcctcgaata ccgcgtgccg gttcaccagg aaattcacgg
     2281 ttgcgctcag cagccgggcc acgacgaccg ccgtgagcag gttcccggtg atcgcgtacc
     2341 aggtgaacag cacggtcgcg tccaccacga agccagccag ggacgaagcc gcgtaggcca
     2401 gcagcggacg gtagatgcgc agggagtcgc gcaccggccg gaaatgcgag gacgcgttgt
     2461 cggcgtggta cacggtggcg atttcgatct gccggatcgg cagtcgatcc cgggtcgccc
     2521 gcagcagcag gttgagctcg tattcgaacc ggacccctgg cacggcggac gcccagtcaa
     2581 gcagcccggc ggggtagcca cgcagtcccg tttgcgtgtc aggcaggcgg gtgccggtga
     2641 ggaccgtgaa cacggcggcg gtgatccggt tgcccagccg actgcgcagc gggacgcgtc
     2701 cggtgaaccg gcggacgccg agcaccaact cgccggagtc ccgcacggcg gccgcgaccc
     2761 gcaggatgtc gagcaccgtg tgctggccgt cggcgtcggc gcagaccacc ggctcgtccg
     2821 ggtagagatt gcggatgtag ccgaagccgg tacgcagggc gacccctttg ccgcgattca
     2881 cctcgtggac gagtacttcg gcgccggcgc gggccgcgtc cgcgaagatg ccggcatagt
     2941 cggcgccact gccgtcgtcg accaccagca ccttgagatc cggcacgtgg cccagttcgg
     3001 ccaccaggtt ggtgagccgc tcgtcgggtt catagctcgg tatcagcacg atcatggctc
     3061 agcccgccac gtagaggatg tcagaggtgc cgcgctcgcg tccgcggccg agtgggttat
     3121 tcaccagcga gccgttgaac accatcgcgg acgagccgcc gccgtcgagg ttgtaggcca
     3181 ccttggcgcc ctcgtccacg aacatctggg cgaactcgga catcgaggcg ccgcggctgt
     3241 agccggtgct gcgtccgtcc accacgatga agaggaagtg gttggacgcg atcacgccca
     3301 cgccggtgcg cggctggttg ccctgcaccg agtggttgcc gaagttggtg tcgatctcga
     3361 ggttgtcgat cccggcgatg acttcaccgt tctcgaccag cccggggccg aaggagaggg
     3421 tctgccagac cccgtcggcg agcagctcgg cggcgttggt cttggtctcg tcgtagaggc
     3481 tcatcgaacc gtcggtgcgg atcgccatcc cctggcgggc accctggtca cggtaggcga
     3541 ccccgttgcg gatcacgatg ccggtgtcgc gaaatccgta gtagtcgccg ttgatggcca
     3601 gcatcgcacc ggtctgggct gcgatcactg acggattcgc gacaatgttc tcgccgaacg
     3661 agtcgttggc gaaggccgag cgcacgatcg tggcatcggt caccgtgatg tcggcgacga
     3721 accaggtgag caggctgttg ccgctcccgg aagtgcgctt gctgatcttg atggacgcgg
     3781 tgtcggacgt gtacgcgtcc gcggtggcgg taccgctcgc tggctgcgcg acggtggcgt
     3841 tggccggctg ctgggcggac gcgtccgtca cttcgacgtg ctcgatcacg taccggttca
     3901 gcgcccagcc ggtgccgccg gcggccgcta gcagggtgcc gccaccggcc accaggaacg
     3961 tccgccgagc gaacggacgt cgagtcgggc cctgcgcgtc tgcctgtacg tcgggtagag
     4021 gctccgtcga aatcatcatg taaccagttg accgggcaac cttgtgcgcc tcctgtggcc
     4081 acccccagcc cagccgatgg atcggcggac ttcccctccc cgaccggcac gcgtccgggc
     4141 gcagcagttc gcgccagcga aacggacaca agccagggaa ggttcgctgg cgttggtccg
     4201 tttcgctggc gcgaagtcgg gggtcgcggg acgggggcga gcgtcaggga cggcgtccgc
     4261 cgcccgggcc acnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntggccgcc
     4381 gacgtagacg tcgtaggact cgccggtctt caggtcgggg tcggagagca ccaccgactg
     4441 cacctgcttc accgcggtgt aggacgcgat cacggtcccg tccgtcacga tgctgatcgt
     4501 ctggccggcc gcgaccgcct gatcgagggt gaccgcgacc cagccttgcg tcgactccgt
     4561 gccgggcgtc tcggccatgc ctgcgctgcc ggccgccagc accgtgccgc cggtgacggt
     4621 gatgccgccg ttggagtcga gtgcaccatt gccggagttc gtcggtccgg agaccacggt
     4681 ggtcccgcca gtgatcacca gcgcgccgtt ggagtcgagg ccatcaccgt ccgcattgac
     4741 caacagcgtg ccgccgctga tcgtgagcag cgagccgtcg tcctgcatgc caccgccccc
     4801 gcgtccgccg gtgtcggtgc tggtgcccga ggtggcgttc agcccgtcat cggacgcggt
     4861 gacgtccacc tcaccaccgg cgatgctgat cgtggcgccc tcgatcgcct cgttcgacct
     4921 ggtgacggtg agcttgcccg aattcaccgc caggtcgccc tcggcgtgga taccgtcgtc
     4981 gccggccgcg atctcgacag tgccgccgtc gatgctcacg tagccgacgg tgtcgtcgtc
     5041 ctcgttgtca gacttcaacc cgtccccttc ggcggtcacg ttcacagtcc cgtccgcgat
     5101 gatcaggtag tccttgccgc ggatgccatc gtccttcgcg ctgacggtga tcgtgccgct
     5161 gaggatcacc aggccgtcct tgctggcaat gccatcgttt gtgttgccgg tgaccgtgag
     5221 cgcgccggta ccgccgatgg tcaggtcggc catcgagaac agcgctgcgt tcggcgcatc
     5281 gtccgcggac gtgtcgtagc cggtgccgtc gacgagagtg ttggtggagc cgtcggcgag
     5341 gatcaccacg gcctcgtccg ctgcggtgat caccaccgcc gaaccggatg tcgaaacgat
     5401 ggcagcgttg tcgaggacca gctttacctt gccctcggcc tcgctgttca ccacgacctg
     5461 gccgttgctg agcgatccgg tcagcacgta ggtgcccggg gcgctgatgg taatcacgtc
     5521 gccgtcgacc ttgacgccgt ccgacgagct ggccttcgac gccccgtccg cgagcgtgat
     5581 cgtgaccgca tcggacgcgt cgtagtcggc gtcgttcgcg tccgcgtggc tctcggcatt
     5641 gtcggccagc gcggcagcgg cggacgcgac catcgtcgtc gagccgctgg cggtctgggt
     5701 ggtggtctgg gtggtgccgg tagtcgttga actggtcgtt gtggtcgacg tgcaggcggt
     5761 gaagacgagg ccagtcgcca atgcgacgat gccggccgtc agctttcgga tcggggtaat
     5821 ggtcatatca gttttcgctt tcattccggg ttcagttgat tcagggcccg gcgccatttc
     5881 agctcgggca ggtcctcgcc aagggccgcc aggccggcgc cgtacttgct cacacagctc
     5941 gggcggacgc cgctgtacca cagcgcccgg tcaatggcgg acgcggtggc gccgcccttg
     6001 gtctcgatga tcacgctgcc cggcgtcgcc agcgagtgct cgctgcccgg gcgtcgccag
     6061 accaggttcg agtcgatggt ggcgcggctg acctggtcgg aagttcgcga actcaccagc
     6121 agcgtgctgc ggtcgtagcc ggtgtgcagg gtgggttgga gcgccgccac gtccactccg
     6181 aagacttcct gggcggcgag ggttgcggcg atgaagtcca gcccgacggt ggtgagccga
     6241 ccggcgtcgg caaggtcgta gggcatccgg tccttcaccg tggtgccacg cggtcctcgc
     6301 gtcttgacct ccagccacgc ctcgccggaa tcgcggtaga cccgcgtccg caccttgaag
     6361 cgacggcgtc gaccccgccc ggcaaggaga aacgcgtcca gttccggggt gtcgaagtag
     6421 gtcgagcggt aggcgaagcg gcggcgtccg gcgatgctga gcacgcgcag gtcggcgtcc
     6481 gcttggccga ggagcgtggg gacggcgtcc gcttcgacca ggtacttgcg gtcgatgcgg
     6541 gtctgcagct cggcttggct gagtagttgc tcgagggtga tcgtgggcaa ctcgtcgagg
     6601 ctgcgcggac ggactgcggc gacgggttcg gcgtaggcga gggcggtcat cgggccaccg
     6661 ccgcaaccgg cgccggcttg gcggcgcgaa cgaagcgaac gtcgaccagg gtggtgtcgt
     6721 tgaccagatc ggtcttgacg acggtgatcg cgtccacatt gccgccgaga agggactcaa
     6781 cccgggcgcg gagggcgacc tcgtcggtgt aggcacgatc gagctggatc tgctggcgct
     6841 cggtgcgctt ggcgatcgcg gggtggtcgc cgaggtagag cgtggcgacc accgcggtca
     6901 tcagtccgag cacccagacc agatcgaggc cggtgctgcc gccgagcagg ccgagggtca
     6961 gcgccgcgaa gtagtaggcg acctcgtgct ggcgaagctc ttcggaccgc agccggatga
     7021 tcgacaggac gccgaacaga ccaagcccca cccccaccga cagtgtcgtg gacgaaaggg
     7081 tggccatcag ggcgagcacc ccgacgttga ccacgaggaa cgcgacgatc aggtcgcgcc
     7141 gctggtggcg ccgcaggaag agaccgaaga ccaggacgac gatggcgacc aggtcggctg
     7201 cgatgag
//
LOCUS       DAOPRQ010000101         7114 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_8721_length_7114_cov_12.835246, whole genome shotgun sequence.
ACCESSION   DAOPRQ010000101 DAOPRQ010000000
VERSION     DAOPRQ010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 7114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7114
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8721_length_7114_cov_12.835246"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="PKJ61_11000"
     CDS             complement(<1..296)
                     /locus_tag="PKJ61_11000"
                     /inference="COORDINATES: protein motif:HMM:TIGR02683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOC14390.1"
                     /translation="MVEIVRSGTFDRWLTKLNDRAAVARVMIRINRLAAGNPGDVKPI
                     GHGISQLRIPYGPGYRIYYVHDGDHVILLLAGGDKSTRTPTSVKPTKSPPIGGEA"
     gene            928..1830
                     /locus_tag="PKJ61_11005"
     CDS             928..1830
                     /locus_tag="PKJ61_11005"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOC14391.1"
                     /translation="MASITASSALEGAIVADQARAKQIIDGKAAQLRNRSEQELAGYR
                     AALDYLFTEAWHPLNAGLLLHVHRLLWGHTAAEGGKFKVDDNLVVDRSATGDTEVRFV
                     PVPARLTPQCTTDLVDRYTEETLRGAHHPVLLIGLFVLDLLTIHPFADGNGRVVRAVT
                     NALLDDAGYGVGRYVSLEQIIANSAEAYYAALLASTRGWHDDAHDPWPWLGYFVSTLT
                     TAYETFESRASSDRSTGTKQDRVRDYVVNHAGDIFKISDIRVTLPGISDPTIRLALES
                     LKREGRITPEGTGRSAVWRKTRST"
     gene            complement(1969..2271)
                     /locus_tag="PKJ61_11010"
     CDS             complement(1969..2271)
                     /locus_tag="PKJ61_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014847605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HOC14392.1"
                     /translation="MTTLNEITARRKPRRERVEVHKERMLAQARAWRLRELRERFEIT
                     QVELAGRLQVSQNRVSTIERGDIDRAQVDTLRRYVEALGGTMTLEVHVGDETFTIA"
     gene            complement(2268..2681)
                     /locus_tag="PKJ61_11015"
     CDS             complement(2268..2681)
                     /locus_tag="PKJ61_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014847604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOC14393.1"
                     /translation="MPPVTPSRFYNLSVVLRWVWEVDVTLAQGWLLGHDEDDYALVIA
                     ALEILQSAGPMLGRPLVDTLVGSRHKDMKDLRPGSRGRSEVRILFAFDPRRRAILLVA
                     GDKAGNWRRWYTKNIPVADDLFDDHLEKQRRRRTT"
     gene            2791..3657
                     /locus_tag="PKJ61_11020"
     CDS             2791..3657
                     /locus_tag="PKJ61_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HOC14394.1"
                     /translation="MLANYLVGLREGLEASLVVVILVAHLVRTNRRELLTSIWLGVGV
                     AVAISLAFGALLTFGPRGLTFEAQEIIGGCLSILAVGFVTWMVLWMATAARGLSGELK
                     GHVDAAALAGRGSLVVVAMLAVGREGLETALFLWAATRAATGGSQSAVIPLVGALLGL
                     LTAVAIGWLVYRGALRLNLARFFSWTGGFLILVAAGVLSYGVHDLQEAGVLPGLNTLA
                     FDVSNVIDPSTLLATVLKGVFNFSPATTVAEAFAWVAYVIVVLPLFVRRLRAGTTAPS
                     SSTASTPSGVSS"
     gene            3654..4829
                     /locus_tag="PKJ61_11025"
     CDS             3654..4829
                     /locus_tag="PKJ61_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M75 family protein"
                     /protein_id="HOC14395.1"
                     /translation="MTTRLRPAAVVILAAGLALGLAGCTDNSNAVAAGGIAAPGALTV
                     VSTDDGCQVSAVQAPSGTLTFSVANKGSKVTEFYLLAQDRLRIVGEVENIGPGLTRDL
                     VLRAAPGSYFTACKPGMKGDGIQAAFTVTDSGTPLAAAGADADLVAQANAAYAAYVKD
                     QTEHLVTKTDAFAAAYTAGRDDEARTLYPAARVHWERIETVAESFGDLDPRMDAREAD
                     LEPGQKWTGWHVMEKDLWPPKTGYTALTTAQRKEMADTLVTDTATLYERTRSMTFTAD
                     QIGNGAKGLLDEVATGKVTGEEETWSHTDLWDFQANVDGARVAWEGLRPLLKKRDAAL
                     DTQIEQRFTALQKLLDAHREGDGFVLYTSLDPAEVKALSDAVNALSEPLSKLTAAVV"
     gene            4839..6245
                     /gene="efeB"
                     /locus_tag="PKJ61_11030"
     CDS             4839..6245
                     /gene="efeB"
                     /locus_tag="PKJ61_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HOC14396.1"
                     /translation="MTDSTTGTSSGAPADSASGPASGPASGPASGPASGPASGPVTEA
                     WRYSRRRFLGAAGAVAGAAGVAGFAGGLLTGRGGEAAPAPASGTTGPAHPFHGEHQAG
                     ILTPAQDRLHFAAFDVTTSSRDELIGLLRAWTSAAAELTQGRAVSSGSAVGYDAPPLD
                     TGEATGLQASRLTITIGFGPTLFRTEDGRDRFGIAGRQPKLLTRLPHFPADDLDPRRS
                     DGDLAVQACADDPQVAVHAVRNLSRIAFGAAGIRWAQLGYGRTSSTSTAQETPRNLFG
                     FKEGTANIKLEETDAATQHVWVAPEDDPGAAWLAGGSYLVARRISMHIETWDRTSLRE
                     QEAIMGRDRRDGAPLSGGNEFTKPDFAAKGGDGRPLIPEVSHVRLAHPDTNGGHRMLR
                     RGYNFTDGSDALGRLDAGLFFLAFVRDPATQYVPVQNRLSKSDVMMEYIKHTGSALFA
                     VPPGARPGEYLGQSLFST"
     gene            complement(6760..6835)
                     /locus_tag="PKJ61_11035"
     tRNA            complement(6760..6835)
                     /locus_tag="PKJ61_11035"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6800..6802),aa:Arg,seq:acg)
BASE COUNT         1130 a         2564 c         2302 g         1118 t
ORIGIN      
        1 gcttctcctc caatcggcgg cgatttggtg ggcttgacgg atgtcggcgt cctggtggac
       61 ttgtccccgc cggcgagcag caggatcacg tggtcaccgt cgtgtacgta gtagatgcga
      121 tagccggggc cgtaggggat ccgaagctgg gatatgccgt gcccgatggg tttgacgtcc
      181 ccggggtttc cggctgccag tcggtttatc cggatcatta cgcgcgcgac agcggcgcgg
      241 tcgttgagtt tggtgagcca tcggtcgaac gttccggatc ggacaatctc gaccatgtgt
      301 caactgtagt tcacaccggc gagagcgggc aacttcagac cactcccttg gcgtcgggtt
      361 tgatctgggt cgcctcgcga tgccttgccc ggttccccgc gacacgatgg gcggacaatt
      421 gacgacgcca ccctcaaggc gctgtccggc caaacactcc aatccgccgc cgccgacgaa
      481 tcggtgccgc gggcaccagg tcagtacgcg cagtacgggg atcgcttagc gtgtgcggga
      541 gcttggcttc gctggacggg gagcgactgc cctcatccgg tgcctcgcaa cccttgagtc
      601 tggccaaggt cggcgagccg aggcagtgca cgagggcggc cagaacgcac acggctgacc
      661 tggccggcgc atggcaaccg gccgaagcga gcaacgggtc gaggctaagg aatccactca
      721 tgacgctaag atcccacctc gttagcatga ttaaacgttg agttgggagg ttcggtgccg
      781 tcgttcactg ccgtggacag actcctgcag tcggtccccg gccctcttgt cacccggctg
      841 agcaggatcg acacaggccg tggccgcgaa cagctgtatc gccatcagct cccccaactc
      901 ctcaccgagc tggcggcacg ggcccgcgtg gcgagcatca ccgcctcgtc cgctctcgaa
      961 ggtgcgatcg tcgccgacca agcccgagcc aagcagatca tcgacggcaa ggcggcccag
     1021 ctgcgcaacc gcagcgagca ggagctcgca ggctaccgag ccgccctgga ctacctgttc
     1081 accgaggctt ggcatccgtt gaacgccggc ctgctgctcc acgtgcaccg cttgctctgg
     1141 ggacacacgg cggccgaggg tgggaagttc aaggtcgacg acaacctcgt cgtcgaccga
     1201 tctgccaccg gggacaccga ggtgcggttc gtacccgttc ctgcccggct cacgccgcaa
     1261 tgcaccacgg acctggtgga ccgctacacc gaggaaactc tccggggtgc acaccacccg
     1321 gtcctgctca tcggcctgtt cgtcctggac ctgctcacga tccatccgtt cgccgacggg
     1381 aacggccgcg ttgtgcgcgc cgtcaccaac gcgctgctcg atgacgccgg ctacggagtg
     1441 ggtcgctacg tctcactgga acagatcatc gccaactccg ccgaggccta ttacgcggcc
     1501 ctgctcgcct ccacccgcgg ctggcacgac gacgcccacg acccctggcc gtggctgggc
     1561 tacttcgtgt ccaccctgac cacggcatac gaaaccttcg agagccgggc gtcttcggac
     1621 cgctcgaccg gcaccaaaca ggatcgtgtc cgcgactacg tcgtcaacca cgccggcgac
     1681 atcttcaaga tcagcgacat ccgcgtgacc ctgcccggga tcagcgaccc gacgattcgg
     1741 cttgcgctcg aaagcctgaa acgagaaggg cggatcacgc cggaggggac cggccgttct
     1801 gccgtatggc gcaagacacg gtcgacgtag cgcgaacggc acttcacgcc accacggatc
     1861 aggtgtcgcc gatcgatgca gccgtcactc catcgtcttc ggtccaccga tcggggtggc
     1921 tagagccgac acccgcccaa gccacggcat acccaggccg cgccaaaatc aggcgattgt
     1981 gaaggtttcg tcgccgacat ggacctcaag agtcatcgtg ccgccgagag cctcgacgta
     2041 gcggcgcagg gtgtcgacct gcgcacggtc gatgtcgcca cgttcgatcg tggacacgcg
     2101 gttctggctc acctgcaacc gtccagccaa ctcgacctga gtgatctcga accgttcgcg
     2161 caactcccgc aggcgccacg cccgagcctg agcgagcatc cgctccttgt gcacctcgac
     2221 cctctcgcgg cgcggcttac gccgcgcagt tatctcgttc agcgtggtca tgtcgtcctc
     2281 cttcggcgct gcttttccag gtggtcatcg aacaagtcgt ccgcgaccgg gatgttcttg
     2341 gtataccaac gacgccaatt gcctgccttg tcaccggcca ccaggaggat cgcccgccgc
     2401 cgagggtcga acgcgaacag gatccggacc tcagaacgcc ctcgcgaccc aggccgcaag
     2461 tccttcatgt ccttgtgccg cgaacccacg agggtgtcga ccaagggtcg tcccagcatg
     2521 gggccggcgc tctgcaagat ctccagcgca gcgatcacca gggcgtagtc gtcctcgtca
     2581 tgacccagca accagccttg agccagtgtg acgtcaacct cccagaccca ccggagtaca
     2641 acactcagat tgtaaaacct agaaggtgta actgggggca cgagggccgg cgctcagact
     2701 caccctgcgg aacgacccga aaaagaccgt gggggacctg ctagcctccg aagtaaggta
     2761 aggcttacct aacttcctga ggctgaggcg atgctggcga actatctggt ggggctccgc
     2821 gagggcctcg aggcgtccct ggtggtcgtc atcctcgtcg cccacctggt gcgcaccaac
     2881 cgccgggagc tcctcaccag catctggctc ggggtcggcg tcgccgtggc gatctcgctg
     2941 gctttcggcg cactgctgac cttcggcccc cgcgggctga ccttcgaggc gcaggagatc
     3001 atcggcggct gcctgtcgat cctcgccgtc ggtttcgtca cctggatggt cctctggatg
     3061 gcgaccgccg cccgcggcct gtccggtgag ctgaagggtc acgtggacgc tgcagcgctc
     3121 gccggacggg gcagcctggt ggtcgtcgcg atgctggccg tcggccgcga ggggctggag
     3181 acggcgctgt tcctgtgggc ggccacccgg gcggcgaccg gcgggtcgca gtccgccgtg
     3241 atcccgctcg tcggcgccct gctcggcctg ctgaccgcgg tggcgatcgg ctggttggtg
     3301 taccgggggg cattgcgcct caacctggcg cgattcttct cctggaccgg cggatttctc
     3361 atcctcgtcg ccgccggggt cctctcctac ggcgtccacg acctgcagga ggccggcgtc
     3421 ctgcccggtc tgaacaccct ggccttcgac gtcagcaacg tcatcgaccc gtcgacgctc
     3481 ctggccaccg tgctcaaagg tgtcttcaac ttctcccccg ccaccacggt cgccgaggcg
     3541 ttcgcctggg tcgcctacgt gatcgtcgtg ctgccgctgt tcgtccgccg attgcgtgcc
     3601 ggcactaccg ctccgtcctc gtccaccgcg tccaccccgt ctggagtctc gtcgtgacca
     3661 cccgcctacg ccctgccgct gtcgtcatcc tcgccgccgg gctggcgctc ggcctggccg
     3721 ggtgcacgga caattcgaac gctgtcgccg ccggcggcat cgccgcgccc ggagccctga
     3781 ccgtcgtctc cacggacgac ggctgccagg tctccgccgt ccaggcaccc tcgggcacgc
     3841 tcacattctc ggtcgcgaac aagggatcga aggtcaccga gttctacctc ctcgcgcagg
     3901 accggctgcg catcgtcggt gaagtcgaga acatcgggcc cggcctcacc cgtgacctcg
     3961 tgctgcgggc cgcgcccggg tcctacttca ccgcctgcaa gccgggcatg aagggcgacg
     4021 gcatccaggc ggcgttcacc gtgacggact ccggtacgcc gctggcggcg gccggtgcgg
     4081 acgccgatct ggtcgcccag gcgaacgcgg cgtacgccgc ctacgtcaag gatcagaccg
     4141 agcatctcgt gaccaagacg gacgcgttcg ccgccgccta cacggccggc cgggacgacg
     4201 aggcccggac gctctacccg gccgcccggg tgcactggga gcggatcgaa accgtcgcgg
     4261 agtccttcgg ggacctcgac ccacggatgg acgcccggga ggcagatctc gaaccgggtc
     4321 agaagtggac cggctggcac gtcatggaga aggacctctg gccgccgaag accgggtaca
     4381 ccgcgctgac cacggctcag cgcaaggaga tggccgacac cctcgtcacg gacacggcca
     4441 ccctctacga gcgcacccgg agcatgacgt tcaccgccga ccagatcggc aacggggcga
     4501 aggggctcct cgacgaggtc gccaccggca aggtcaccgg cgaggaggag acctggagcc
     4561 acaccgatct gtgggacttc caggccaacg tggacggcgc tcgggtggcc tgggagggcc
     4621 tgcggccgct gctcaagaag cgggacgccg cgctggacac ccagatcgag cagcgcttca
     4681 ccgccctgca gaagctgctc gacgcccacc gcgagggtga cggcttcgtc ctctacacca
     4741 gcctggaccc ggccgaggtc aaagccctct cggacgctgt caacgcgctg agcgaacccc
     4801 tgtccaagct gaccgccgcg gtcgtgtgag gagcgaggat gactgacagc acgaccggga
     4861 cctcgagcgg agccccggcg gattcggcgt ccggcccggc gtccggcccg gcgtccggcc
     4921 cggcgtccgg cccggcgtcc ggcccggcgt ccggcccggt taccgaggcc tggcggtaca
     4981 gccggcggcg cttcctcggc gccgccggcg cggtcgccgg tgcagccgga gttgccgggt
     5041 tcgccggcgg cctactgacc gggcgcggcg gcgaggccgc accagccccg gcgagcggca
     5101 cgaccgggcc ggcccaccca ttccacgggg agcatcaggc ggggatcctc acccccgccc
     5161 aggaccggct gcacttcgcc gccttcgacg tcaccacgag ctcccgggac gagctcatcg
     5221 ggctgctgcg cgcctggacg tcggccgccg ccgagctgac ccagggccga gccgtctcct
     5281 ccggcagcgc cgtcggctac gacgcgccac cgctcgacac cggcgaggcg accggcctgc
     5341 aagcgtcccg gctgaccatc accatcgggt tcggccccac cctgttccgc accgaggacg
     5401 ggcgtgatcg cttcggcatc gccggtcggc agccgaagct gctgactcgt ctcccgcact
     5461 tccccgccga cgacctcgat ccgcgccgca gcgacgggga cctcgccgtg caggcctgcg
     5521 ccgacgaccc gcaggtcgcc gtgcacgccg tccggaatct gtcccggatc gccttcggcg
     5581 cggccgggat ccgctgggcg cagctcggtt acgggcggac gtcgtccacg tcgaccgccc
     5641 aggagacccc gcgcaacctg ttcggcttca aggaaggaac tgcgaacatc aagctcgagg
     5701 aaacggacgc cgcgacgcag cacgtgtggg tcgcccccga ggacgaccct ggggccgcct
     5761 ggctggccgg cgggtcctac ctggtcgccc ggcggatcag catgcacatc gagacctggg
     5821 accgtacgtc gttgcgggag caggaagcca tcatgggccg cgaccgccgc gacggcgccc
     5881 cgctgtccgg cgggaacgag ttcaccaagc ccgacttcgc cgccaaagga ggggacggcc
     5941 ggcccctgat ccccgaggtc tcccacgttc ggctcgccca tccggacacc aacggcgggc
     6001 accgcatgct tcggcggggc tacaacttca ccgacggcag cgacgccctc ggacggctcg
     6061 acgccgggct gttcttcctc gcgttcgtgc gcgacccggc cacgcagtac gtcccggtgc
     6121 agaaccgcct ctccaagagc gacgtcatga tggagtacat caaacacacc ggctcggccc
     6181 tgttcgccgt cccgcccggc gcccgcccgg gtgagtatct cgggcagagc ctcttctcga
     6241 cctgacggga cctcacgccg gtccggttcg atgcggtgag cgaaccggcg cgccggttgt
     6301 tgaggcgatg cgcttcgaac cggccggcga caacgggttg ctccgacagc gcggcgcacc
     6361 tggtcgcgag ccagtccgtt gccccctcgt cgtcggcgtc gcagaaggtc agccaccggc
     6421 cacgggcctc gtcgacgccc cgattgcgcg ccggattcac gtatcggccg gcgtgagacc
     6481 tggccacgtc ctcgtcggtt gtgatgtaca gaccagtccg gggtcgtagg tagccgtggg
     6541 gtctagatac gagtacacgg atctcagttc cgccgctggc agcaacacat cgcctacagc
     6601 aagcccgggg gctccccgat gaaacatgcc acctgtgccg acaacaaggt gtctcttgcc
     6661 gcgagcgtgc cgcgatcgct tgcccacggt tcaccgctca tgagcgcatc gctggccagc
     6721 tgcgacggtc cgagcggacc cgccgtcctc gaggtctgct ggcgcgcccg aagggattcg
     6781 aacccctaac cttctgatcc gtagtcagat gctctatccg ttgagctacg ggcgccggtc
     6841 gtgtcgacca ccccaccggg gggcagcgcg acgaggagca agcctaacgg catacccggg
     6901 ttgggaagaa atcgaatcca cggccggaca ccccaccgcc acccccggta tgccgtccgc
     6961 tcacctggcc ggacggcata cccgatgctt gtgaggggac tcacccggcc gggacctctg
     7021 cccggttacg ggccctgggc cccccgggat agcgtggtct cgaccgagac gtctggcacg
     7081 tctctcgggc ggcgttccgt ccgtcccaag cgtt
//
LOCUS       DAOPRQ010000102         7072 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_8796_length_7072_cov_11.700299, whole genome shotgun sequence.
ACCESSION   DAOPRQ010000102 DAOPRQ010000000
VERSION     DAOPRQ010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 7072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7072
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8796_length_7072_cov_11.700299"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..770
                     /gene="sufB"
                     /locus_tag="PKJ61_11040"
     CDS             <1..770
                     /gene="sufB"
                     /locus_tag="PKJ61_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002527055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HOC14397.1"
                     /translation="QFERTLIIADEGSYVHYVEGCTAPIYSSDSLHSAVVEIIVKKNA
                     RVRYTTIQNWSTNVYNLVTKRATCEEGATMEWIDGNIGSKVTMKYPAVYLLGEHSRGE
                     TLSIAFAGEGQHQDAGSKMVHAAPHTSSSIISKSVARSGGRASYRGLVQVQEGAHHSA
                     SSVKCDALLVDTISRSDTYPYVDVREDDVSMAHEATVSKVSDDQLFYLRSRGMGEDEA
                     MAMIVRGFVEPIARELPMEYALELNRLIELQMEGAVG"
     gene            795..1997
                     /gene="sufD"
                     /locus_tag="PKJ61_11045"
     CDS             795..1997
                     /gene="sufD"
                     /locus_tag="PKJ61_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HOC14398.1"
                     /translation="MTVTALPFVAEAAEATVASHLHPVTSFDLADHPIPSGREEVWRF
                     TPLRRLRGLLDGNPSDAHLEWNAELPAGVTVTELPTADAIALGVPAPVDRPSALAVAH
                     GGGAVLVDVPANSQLTDPVVIELSGSGLTQVYGQVVFRIGTNAAATIVLRHTGSAVYA
                     SSVSVLVGEGANVNFVGLQDWADDTVHVGHVGLRVGRDARVRTVTASLGGDLVRLVET
                     AEYDGPGGELEQFGLYFVDAGQHIEHRLFVDHNAPHTRSHVDYRGALQGVGAHSVWVG
                     DVLIRKIAEGISTYETNKNLVLTDGCRADSVPNLEIETGEIEGAGHSSTTGRFDDEQL
                     FYLRSRGIPEEEARRLVVHGFFADIIRRIGVPEVESRLLATVEKELEVIVGAPTSVAS
                     LDEYRAQQ"
     gene            1994..2329
                     /locus_tag="PKJ61_11050"
     CDS             1994..2329
                     /locus_tag="PKJ61_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HOC14399.1"
                     /translation="MTFVRACALAEVPVGSSIAIDFGGEAEIAVVNTDDGYFAIQDLC
                     SHAEVPLSDGDVEGCTIECYMHGSVFDLRTGQPLNLPATIPVPVYPTRIVGDDLYLDP
                     DNPIESQEQ"
     gene            2330..3097
                     /gene="sufC"
                     /locus_tag="PKJ61_11055"
     CDS             2330..3097
                     /gene="sufC"
                     /locus_tag="PKJ61_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HOC14400.1"
                     /translation="MSELRISDLRVEVETESGPKEILKGVNLTVRSGEVHAIMGPNGS
                     GKSTLAYSIAGHPKYKITGGSVTLDGVELTELAVEERAKAGLFLAMQYPIEVPGVSVA
                     NFLRTAKTALSGEAPKVRTWVKDVNAALERVGLDGSFAARSLNEGFSGGEKKRNEIAQ
                     LELLNPKFAILDETDSGLDIDALRIVSEGVNRYTAQGDRGLLLITHYTRILNYIKPDF
                     VHVFVDGRIAEEGGAELADQLEIEGYDRFVKAVKLDA"
     gene            3090..4400
                     /locus_tag="PKJ61_11060"
     CDS             3090..4400
                     /locus_tag="PKJ61_11060"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013161361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HOC14401.1"
                     /translation="MPDSTARSVGASPRLVEPVETPFDVERIRADFPILTRRVGEWPL
                     VYLDSANTSQKPRAVVDAIATHYLEHNANVARAMHTLGGEATEAFEGARNKVANFIGA
                     VPEEVVFTKNASEALNLAAHTLGARLQPGDEIVISVLEHHSNIVPWQLLAERTGAKLR
                     WFDITADGRLDLEAAETDGLINERTRIVSVAWVSNVLGTINPVRQIAGLAHRVGATVV
                     VDASQGVPHLPTDVGVLGADLLAFTGHKMLGPTGVGLLWGSYDLLANLPPFLGGGEMI
                     EIVRMTGSTFAKPPHRFEAGTPPIAQAVGLGAAVDYLSAIGMDAIAAREHQLAEYLLG
                     RLGAVEGVRILGPTEAIDRGAAVSFTLATSDGMEIHPHDVMQFLDARGVAVRGGHHCA
                     RPLHDRLGVTASSRASAYLYTTEAEIDAFGDALDYVRNFFGGVR"
     gene            4397..4849
                     /locus_tag="PKJ61_11065"
     CDS             4397..4849
                     /locus_tag="PKJ61_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013161360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HOC14402.1"
                     /translation="MSVEAMYQEIILDHYKAKHHSGLREPYQAQVTHVNPTCGDEVML
                     RVALDGDRVTDVSYEGVGCSISQASTSVMTDLVIGRTVAEALALHEEFTEMMHSQGRM
                     EPDEDRLEDAVAFIGVAQFPARVKCALLGWSAFKDATAQALATTDREA"
     gene            4851..5225
                     /locus_tag="PKJ61_11070"
     CDS             4851..5225
                     /locus_tag="PKJ61_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016455774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HOC14403.1"
                     /translation="MTHPTRPSDLVNDTFPDDETTARPSEEDVLEAMKDVVDPELMVN
                     VVDLGLVYGVTIDDEHNATIDMTLTSPTCPLTDRLEYDTQLALEGLVNSVTINWVWLP
                     PWTLENITEDGREQLRAIGYNL"
     gene            5446..6423
                     /locus_tag="PKJ61_11075"
     CDS             5446..6423
                     /locus_tag="PKJ61_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016667952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOC14404.1"
                     /translation="MSSHDEQPRDDQETLGGEPAGVPVGHAGTRVEISERRAWSIPGA
                     IAVLGILVILAAAIWLIVLAAMADESGANGTVFGVLGALLLVVGVIGASAITIVGPGD
                     TRVLQLFGRYVGTVRTPGLQLTVPFVVGRKVSVKVRNFETNELKVNDADGNPVNIASI
                     IVWQVADTAKAVFAVEKYTEFVEVQAESALRHVATSHPYDNAGPGEESLRGSTDLVAA
                     ELAAEVAARIAIAGLEVVEARISSLAYAPEIAHAMLQRQQASAVIAAREKIVEGAVSM
                     VQGALRQLEAEDIVALDEERKAAMVSNLLVVLCSSTQATPVVNTGTLYS"
     gene            6429..6677
                     /locus_tag="PKJ61_11080"
     CDS             6429..6677
                     /locus_tag="PKJ61_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14405.1"
                     /translation="MTEPTDAGQAGGRERKPVLLRLDPAVHAALAQWAADDLRSLNAH
                     IELLLRQALVKAGRKPRDAAPIPKRGRPAKDARGAGDA"
BASE COUNT         1160 a         2296 c         2458 g         1158 t
ORIGIN      
        1 gccagttcga gcggacgctg atcatcgccg acgagggctc ctacgtgcac tacgtggagg
       61 gctgcaccgc gccgatctac tcgtccgact cgctgcattc ggcggtggtc gagatcatcg
      121 tgaagaagaa cgcccgggtg cggtacacga ccatccagaa ctggtcgacc aacgtgtaca
      181 acctggtcac caagcgcgcc acctgtgaag agggcgcgac gatggagtgg atcgacggca
      241 acatcggttc caaggtgacc atgaagtacc ccgcggtcta cctgctgggc gagcattccc
      301 gcggcgagac gctctcgatc gcgttcgccg gcgaaggcca gcaccaggac gccggttcca
      361 agatggtgca cgccgccccg cacacctcca gctcgatcat ctccaagtcg gtcgcccgct
      421 ccggcggacg cgcgtcctac cgcggcctgg tgcaggtgca ggaaggcgcg catcactcgg
      481 ccagttcggt gaagtgtgac gcgctgctgg tggacaccat ctcgcgctcg gacacctacc
      541 cgtacgtgga cgtccgcgag gatgacgtgt cgatggccca cgaagccacc gtttcgaagg
      601 tgagcgacga ccagctgttc tacctccgca gccgcggcat gggtgaggac gaggcgatgg
      661 cgatgatcgt gcgcggcttc gtcgagccga tcgcccgcga gctgccgatg gagtacgccc
      721 ttgagctgaa ccggctgatc gaactgcaga tggaaggggc cgtgggctga gcccgcgcca
      781 aagcgactag aaagatgact gtgacagcac tccctttcgt ggccgaagcg gctgaggcga
      841 ccgtcgcctc gcacctgcac ccggtgacgt ccttcgacct tgccgaccac ccgatcccga
      901 gcgggcgcga ggaggtgtgg cggttcaccc cgctgcgccg actgcgtggc ctgctggacg
      961 gcaaccccag cgatgcccac ctcgagtgga acgccgagtt gccggccggg gtgaccgtga
     1021 ctgagctccc gaccgcggac gcgatcgcgc tcggcgtgcc ggcgccggtg gaccgtccgt
     1081 ccgcgctggc agtggcccat ggcggcgggg cggtgctggt ggacgtgccg gcgaacagcc
     1141 agctgaccga cccggtggtg atcgagctgt ccggctccgg gcttacccag gtctacggcc
     1201 aggtggtctt ccggatcggg accaacgcgg ctgccacgat cgtgctgcgg cacaccggct
     1261 cggcggttta cgcgtccagc gtctcggtgc tggtcggaga aggtgcgaac gtgaacttcg
     1321 ttggcctgca ggactgggcc gacgacaccg tccacgtggg tcacgtcggc ctgcgagttg
     1381 gccgggacgc gcgcgtccgt acggtcaccg ccagcctcgg cggtgatctc gtccggctcg
     1441 tcgagaccgc cgagtacgac ggtcccggcg gcgagctgga gcagttcggc ctgtacttcg
     1501 tggacgcagg gcagcacatc gagcaccgcc tgttcgtcga tcacaacgcc ccgcacaccc
     1561 gcagccacgt cgactaccgc ggtgcgctgc agggcgtggg cgcccactcg gtgtgggttg
     1621 gcgacgtgct gatccgcaag atcgccgaag gcatcagcac ctacgagacc aacaagaacc
     1681 tggtgctcac cgacggctgc cgcgccgatt cggtgccgaa cctggagatc gagaccggcg
     1741 agatcgaggg cgccggccac tcgtccacca ccggacgctt cgacgacgag cagctgttct
     1801 acctgcgctc gcgtggcatc ccggaggagg aagcccgccg gctggtggta cacggcttct
     1861 tcgccgacat catccgccgt atcggcgtgc ccgaggtgga gtcgcggttg ctggccaccg
     1921 tggagaagga actcgaagtg atcgtcggtg cgccgacgag cgtcgcgtcc ctcgacgagt
     1981 accgggcaca gcagtgacct tcgtccgggc ctgcgcgctg gccgaagtgc cggtcggctc
     2041 ctcgatcgcg attgatttcg gtggggaagc cgagatcgcg gtggtgaaca cggatgacgg
     2101 ctacttcgcg atccaggacc tctgctcgca tgccgaggtg ccgctgagcg acggcgacgt
     2161 cgagggttgc accatcgagt gctacatgca cggctcggtg ttcgacctgc ggaccgggca
     2221 gccgctgaac ctgcccgcca ccatccccgt cccggtatat ccgacccgga tcgtcggcga
     2281 cgacctctac ctagacccag ataatccgat cgaatcccag gagcagtgaa tgtcagaact
     2341 ccgcatcagc gaccttcgtg tcgaggtcga gaccgagagc ggtccgaagg agatcctcaa
     2401 gggggtcaac ctgacggtgc gcagcggcga ggtgcacgcg atcatgggtc cgaacggctc
     2461 cggcaagtcg accctggcct actcgatcgc gggtcacccg aagtacaaga tcaccggcgg
     2521 ctcggtgacc ctcgacggcg tcgagctgac cgagcttgcc gtggaggagc gcgccaaggc
     2581 cgggctcttc ctggccatgc agtacccgat cgaggtgccg ggtgtctcgg tggcgaactt
     2641 cctgcgcacg gccaagaccg cgctctcggg tgaggcgccg aaggttcgca cttgggtgaa
     2701 ggatgtgaac gccgcgttgg agcgggtggg tctggacggg tcgttcgctg cccgctcgct
     2761 gaacgagggc ttctccggcg gtgagaagaa gcgcaacgag atcgctcagc tggagctgct
     2821 caacccgaag ttcgcgatcc ttgacgagac cgactccggc ctcgacatcg acgcgctgcg
     2881 gatcgtctcc gagggcgtca accgctacac cgcccagggc gatcgcggcc tgctgctgat
     2941 cacgcactac acgcggatcc tgaactacat caagcccgac ttcgtgcacg tcttcgtgga
     3001 cggccggatc gccgaggagg gtggcgccga gctcgccgac cagctggaga tcgagggcta
     3061 cgaccgcttc gtgaaggctg taaagctcga tgcctgattc gactgctcga tcggtcgggg
     3121 ccagcccccg gctggtcgag cctgtcgaga cccctttcga cgtcgagcgc atccgggccg
     3181 acttcccgat cctgacccgc cgggtggggg agtggccgct tgtctacctc gactcggcca
     3241 acacgtccca gaagccccgg gcggtggtgg acgcgatcgc gacccattac ctggagcaca
     3301 acgccaacgt ggcgcgcgcg atgcacaccc tcggcggtga ggcgaccgag gcgttcgagg
     3361 gcgcccgcaa caaggtggcg aacttcatcg gcgcagtgcc ggaagaagtc gtgttcacca
     3421 agaacgcgtc cgaggcgctc aacctggctg cgcacaccct cggcgcaagg ctgcagccgg
     3481 gcgacgagat cgtgatttcg gtgctcgagc accactcgaa catcgtgccg tggcagctac
     3541 tggccgagcg caccggcgcg aagctgcgct ggttcgacat caccgcggac ggccggctcg
     3601 acctcgaagc ggccgaaacc gacggcctga tcaacgagcg cacccggatc gtctcggtcg
     3661 cgtgggtttc caacgtgctc ggcacgatca accccgtccg gcagatcgcc ggactggccc
     3721 accgggtggg cgcgaccgtc gtggtggacg cgtcccaggg cgtgccgcac ctgcccaccg
     3781 atgtgggtgt gctcggtgcc gatctgctgg cgttcacggg ccacaagatg ctcggcccga
     3841 ccggggtcgg cctgctttgg ggctcctacg acctgctcgc caacctgccg ccgttcctcg
     3901 gtggtggcga gatgatcgag atcgttcgga tgaccggctc gacgttcgcc aagccgccgc
     3961 accgcttcga ggcagggacg cccccgatcg cgcaggcggt cgggttgggc gcggccgtcg
     4021 actatctttc ggccatcggc atggacgcga tcgccgcgcg tgagcatcag ctggccgagt
     4081 acctgctcgg ccgtctcggc gcggtcgagg gggtgcggat tctcggtccg accgaggcga
     4141 tcgaccgggg cgcggcggtt tcgttcacgc tggcgacctc ggacgggatg gagatccatc
     4201 cgcatgacgt catgcagttc ctggatgccc gtggcgtcgc ggttcgtggt ggccaccact
     4261 gcgcgcgtcc gctgcacgat cggctgggcg tgaccgcgtc cagtagggct tcggcgtacc
     4321 tgtacacgac cgaggccgag atcgatgcgt tcggcgacgc gctggattat gttcgcaact
     4381 tcttcggggg tgtccggtga gtgttgaggc gatgtaccag gagatcattc tggaccacta
     4441 caaggccaag catcacagtg gcctgcgcga gccgtaccag gcccaggtga cgcacgtgaa
     4501 ccccacctgc ggggacgaag tgatgctccg ggtggcgctc gatggcgatc gggtgaccga
     4561 cgtctcgtac gagggggtcg gctgctcgat ctcgcaggcg tccacctcgg tgatgaccga
     4621 cctggtgatc gggcggacgg tggccgaggc tctcgcgctg cacgaggagt tcaccgaaat
     4681 gatgcattcc caaggccgga tggagcctga tgaggatcgt ctggaggacg cggtcgcgtt
     4741 catcggggtg gcccagttcc cggctcgggt gaagtgcgcg ctgctgggct ggtcggcgtt
     4801 caaggacgcg accgcccagg cgctggcaac aaccgatcga gaggcatgag atgacccacc
     4861 ccacgcgtcc gtccgacctg gtgaacgaca ccttcccgga cgatgagacc accgcccggc
     4921 cgtccgagga ggacgtgctg gaggccatga aggacgtcgt cgaccccgag ttgatggtga
     4981 atgtggtcga cctcggcctg gtctacggcg taacgatcga tgacgagcac aatgccacga
     5041 tcgacatgac cctgacctcg cccacctgcc cgctgaccga ccggctggag tacgacaccc
     5101 agcttgcgct cgagggtctg gtgaattcag tgacgatcaa ctgggtgtgg ctgccgccgt
     5161 ggaccctgga gaacatcacc gaggacgggc gcgaacagct gcgtgcgatc ggctacaacc
     5221 tgtagaaacc gctcagctgg tcgagcctgt cgcgacccat tgcggtccga cccacggctg
     5281 gtcgagcttg tcgagacgtc tgcggctggc attcggttgg tcgagcttgt cgagacccgt
     5341 tgcggttcga cctgcggctg gtcgagcttg tcgagaccac ttccgccaca gctagaaatc
     5401 tgatatcact tagatatcag attctttggg gaggagcccg acaccatgtc cagtcacgac
     5461 gagcagccgc gagacgatca ggagaccctg ggcggcgagc ccgccggggt gccggtgggt
     5521 cacgccggta ccagggtgga gatcagcgag cggcgtgcat ggtcgattcc gggagcgatc
     5581 gcggtgctgg gaatcctggt gatccttgcc gcggcgatct ggctgatcgt gctggccgcg
     5641 atggccgacg agagcggtgc taatggcacc gtcttcggcg tgcttggcgc actgttgctg
     5701 gtggttggcg tcatcggagc gagcgcgatc acgatcgtcg gcccgggcga cacccgagta
     5761 ctgcagctgt tcgggcggta cgtgggcacg gtccgcacgc ctggactgca gctgacggtg
     5821 cccttcgtgg tcggccgcaa ggtctcggtg aaggtgcgca acttcgagac caacgagctg
     5881 aaggtgaacg atgccgacgg aaacccggtg aacatcgcct ccatcatcgt ctggcaggtg
     5941 gccgacaccg ccaaggcggt cttcgcggtc gagaagtaca ccgagttcgt cgaggtgcag
     6001 gccgagtcgg cgctgcggca cgttgcgacc agccacccct acgacaacgc gggtccgggg
     6061 gaggagtcgc ttcgcggttc caccgacctg gtggcggcgg aactcgccgc cgaggtcgcc
     6121 gcgcggatcg cgatcgcggg cctggaagtg gtggaagcac gcatctccag cctcgcctac
     6181 gcgccagaga tcgcccacgc catgttgcag cgccagcagg cgtccgcggt gatcgcggcc
     6241 cgcgagaaga tcgtcgaggg tgcggtcagc atggtgcagg gagcgctacg ccaactcgag
     6301 gccgaggata tcgtcgcgct cgacgaagaa cgcaaggctg ccatggtttc gaacctgttg
     6361 gtagtacttt gtagttcaac tcaggctacc cccgtggtca acaccgggac gctttacagc
     6421 tgagtccgat gaccgagcca acggacgcgg gccaggccgg cgggcgggaa cgcaaacccg
     6481 tcctgctgcg cctggacccc gcggtacacg cggcattggc tcagtgggcg gccgatgacc
     6541 tgcgctcgct caacgcccac atcgaactgc tgctgcgaca ggccctggtg aaagcgggac
     6601 gcaagccccg ggacgcggcc ccgatcccca agcgcggccg cccggccaag gacgcccgcg
     6661 gcgctggcga cgcctgaggc ctgggccggt cgtgaagtgc agcctgcacg gctgtgacgc
     6721 tcaggttcgg ccggcggttc gcgccgccct ccctgaacct gtcgaggatc ggggcagggg
     6781 cttcgtcagg ctcagccggc gttggctggt cgggcgtgtt ggaggtcgac cgttccccgg
     6841 ggcggtcgag ggtccgagcc tttgggggtc gtgtccgcgg gggtcgagct tgccgagtgc
     6901 cagccgaccg cgtggagcgc tagccctgtg tgctggcggt cgagggctat ggctggctca
     6961 ggcgccatct tgtgggcggc tcggccagcg cccgccgcca cgacccccgc ggcggccctt
     7021 cgtgacgcgt tcgctcgttc ctcgcgaacg ctcctcaggg accgattggg gg
//
LOCUS       DAOPRQ010000103         7010 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_8903_length_7010_cov_11.556003, whole genome shotgun sequence.
ACCESSION   DAOPRQ010000103 DAOPRQ010000000
VERSION     DAOPRQ010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 7010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7010
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8903_length_7010_cov_11.556003"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="PKJ61_11085"
     CDS             complement(<1..190)
                     /locus_tag="PKJ61_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002526735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HOC14406.1"
                     /translation="MLPVLEISGSINMIGYALATLGPAIGVAWIFASVINGTARQPEA
                     RGAMMGTAWIGFAVVEALA"
     gene            complement(222..1034)
                     /gene="atpB"
                     /locus_tag="PKJ61_11090"
     CDS             complement(222..1034)
                     /gene="atpB"
                     /locus_tag="PKJ61_11090"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HOC14407.1"
                     /translation="MRGLIGLIVPLEGDAHGWPPGTHSFASRPLFPGIDPEGAWLNNH
                     VAQAVIGAILVIAFWLWMAHGQKVVPGKKQFAGEFLYNLIRNTIARDILGHDYQKFLP
                     YLVALFSFIFVNNLFGSFFLFMFPTFSKIGFPYGLALLTFVIYNAAGIGKYGLGNYLK
                     RMTLPEGVPVFLWPVIIPLEFISNFVARPFTLALRLFANMFAGHLVIMVFVVGGGLLL
                     ASGEAFYVAAGSMSIVFSFAIFALELLVAALQAYIFTVLSAQYISSAIAEEH"
     gene            complement(1054..1248)
                     /locus_tag="PKJ61_11095"
     CDS             complement(1054..1248)
                     /locus_tag="PKJ61_11095"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HOC14408.1"
                     /translation="MGERSGIDTGLRVVSILISGLIFYGGIGWLLDNWLHTRWWLPVG
                     IIVGTVAGVYLVIARYGRSE"
     gene            complement(1322..1744)
                     /locus_tag="PKJ61_11100"
     CDS             complement(1322..1744)
                     /locus_tag="PKJ61_11100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14409.1"
                     /translation="MAELSLNRRRAQQLLVGGAAGGHLALLLAVLGFAAASGLSAALS
                     AAVAGVVTIAFFMIGQAVQVLVADAPARRVLIASLASYGLRVSVLGGLLMLALANSAQ
                     IAWLDPIAVAVTTIAVVVGWLSVELWTYSRLRIPIYDE"
     gene            complement(1839..2843)
                     /locus_tag="PKJ61_11105"
                     /pseudo
     CDS             complement(1839..2843)
                     /locus_tag="PKJ61_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002513459.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
     gene            complement(2843..3532)
                     /locus_tag="PKJ61_11110"
     CDS             complement(2843..3532)
                     /locus_tag="PKJ61_11110"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOC14410.1"
                     /translation="MSGMAKVWDITADPEGAIEAAAAAVSAGNCIVLPTDTVYGIGAD
                     AFSAHAVQGLLDAKERGRDMPPPVLIADADALRSLADEVSQEMLALADTFWPGALTLV
                     VTAHANLRMDLGDRGQTIAIRVPDHEFTRELLRATGPLAVSSANVSGQPAANTVAEAQ
                     AQLGKRVSVYLDGGPAGAGVPSTIVDLTGRPRILREGKLTRAELVAVVPDLAEAASDP
                     AEQPPQDADQA"
     gene            complement(3529..4368)
                     /gene="prmC"
                     /locus_tag="PKJ61_11115"
     CDS             complement(3529..4368)
                     /gene="prmC"
                     /locus_tag="PKJ61_11115"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012922630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOC14411.1"
                     /translation="MPRSAPLLAAATAELGSAAEARTLLAHVTGVEPSRLLLLDDVDE
                     AAAADYAELIARRAAGEPLQYLTGRAYFRTIEVAVAPGVFIPRPETEVLTGWALERLA
                     KLPGEPVVVELCAGSGAISKAIAVERPGCLQYAVELSETAARLAQQNLSGTGVTLTEG
                     DMADAFPELNGAVDLVIANPPYIPLTAWEYVAEDVRTHEPDLALFSGEDGLDALRVVA
                     EVAARLLRPGGFVCAEHAEVQTDSALDVFAVRGAFTQLADHLDLTGRPRFVAGVRAAN
                     LAG"
     gene            complement(4368..5438)
                     /gene="prfA"
                     /locus_tag="PKJ61_11120"
     CDS             complement(4368..5438)
                     /gene="prfA"
                     /locus_tag="PKJ61_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016456229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOC14412.1"
                     /translation="MFEAAAGLREEYADLERQLADPALHSDLAASRRVGRRYNELSPI
                     VRSLDAHEQLSSDLVAAKELAAEDPSFAEEADAIAAELVTVEEKLTRLLAPRDPNDSA
                     DCLIEIKSGEGGEESALFAGDLFRMYARYAETRGWKTEVLDSQDTDLGGYKSITVAVK
                     APGNGDPENAPYGVLKFEGGVHRVQRVPVTESQGRVHTSAAGVLVMPDVEAAEVEIND
                     EDLRVDVYRSSGPGGQGVNTTDSAVRITHLPSGIVVSCQNERSQLQNKESAMRMLRAR
                     LVARAEDEAASAASAARKSQVRTVDRSERIRTYNFPENRIADHRIGFKAYNLDQVLAG
                     DLSAVITALQAADLAERLGAVG"
     gene            complement(5514..5723)
                     /gene="rpmE"
                     /locus_tag="PKJ61_11125"
     CDS             complement(5514..5723)
                     /gene="rpmE"
                     /locus_tag="PKJ61_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HOC14413.1"
                     /translation="MKSGIHPDYVETQVTCTCGNTFTTRSTAKNGVMHAEVCSACHPF
                     YTGKQKILDTGGRVARFEKRYAKKK"
     gene            5877..>7010
                     /locus_tag="PKJ61_11130"
     CDS             5877..>7010
                     /locus_tag="PKJ61_11130"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF002367.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOC14414.1"
                     /translation="MTALPGNLRLLGLGPERLRDVIDLDRWAFPSSDDLAETLALPSP
                     LPWPRTFGVADADADDRLVAMHASYPFRTCPVPGGRLPVAGLTWVAVHPEARRRGILR
                     SMIGAHLAHCRTWGEPISVLTASEPAIYGRFGYGLAARQVEVRLPRGAALRPVPGGEA
                     LTVRIEHLQVEQHRELIGRLHGAVTRPGWVGRETPELTTAWFADSPMFHPGFESARIM
                     IAERAGVPVGYAVFRRNSSWGPGGPTGTVRVSDFVGADPSATHLLWSRLLDLDLTTEV
                     VVSMLATDDPLLSLLVDVRAVVPTVKDNLWLRLVDVAAALAGRQYAADLDVVLEVSDA
                     MLPSNAGRWRLTASALGSAAVTRTDAEADLALDVRELGSAYLGG"
BASE COUNT         1176 a         2368 c         2375 g         1091 t
ORIGIN      
        1 tggccagcgc ctcgaccacg gcgaagccga tccacgcggt gcccatcatg gcgccacggg
       61 cctcgggctg ccgagcggtt ccgttgatca ccgaggcgaa gatccaggcc acaccgatgg
      121 ccgggccgag cgtcgccagg gcgtatccga tcatgttgat cgaaccgctg atttccagga
      181 cggggagcat gtcgtttcct ttcgagggtt atttggcagg ctcagtgctc ctcggcgatc
      241 gccgaggaga tgtattgggc actcaaaacg gtgaagatgt aggcctgcag cgctgccacc
      301 agcaactcca gggcgaagat ggcgaaactg aacacgatcg acatcgagcc ggccgcgacg
      361 tagaaggcct caccggaggc cagcagcagg ccgccgccga ccacgaagac catgatcacc
      421 aggtggccgg cgaacatgtt ggcgaagagt cgcagggcca gcgtgaacgg ccgggcgacg
      481 aagttcgaga tgaactcaag cgggatgatc accggccaca agaagaccgg cacgccttcg
      541 ggcagggtca ttctcttcag gtagttgccg agaccgtact tgccgatgcc ggccgcgttg
      601 tagatcacga aggtcagcag ggcgaggccg tacgggaagc cgatcttcga gaaggtcggg
      661 aacatgaaca ggaagaacga cccgaacagg ttgttgacga agatgaagct gaacagcgcg
      721 accaggtagg gcaggaactt ctggtagtcg tggccgagga tgtcacgggc gatggtgttg
      781 cggatcaggt tgtagaggaa ttcaccggcg aactgcttct tgccggggac caccttctgg
      841 ccgtgagcca tccagagcca gaatgcgatc accaggatcg cgccgatcac ggcctgagcg
      901 acgtggttgt tcagccacgc accctcgggg tcgatccccg gaaacagcgg acgggacgcg
      961 aagctgtggg tgccaggcgg ccagccgtgg gcgtctccct ctagcgggac gatcaggccg
     1021 atcaggccgc gcatgagacc tcctgtctgg ccgtcactcc gaccgtccgt atcgtgcgat
     1081 aaccagatag acgccggcga cagtaccgac gattattccc acgggcagcc accaccgtgt
     1141 gtgcagccag ttgtcgagca accagccgat tccaccgtag aagatcagcc cagagatgag
     1201 gatgctgacg acccgcaaac cggtgtcgat tccgctccgc tcacccatga ctcttgggac
     1261 tataacagcc gacccgccct cgcttcaccc cggtggcggc aaagcgtcca aagccatcga
     1321 attactcgtc gtagatcggg atccgcagcc gtgaatacgt ccacaactcg acggacaacc
     1381 aacccaccac gacagcgatc gtggtcaccg cgaccgcgat cggatccagc caggcgatct
     1441 gcgcggagtt ggcaagggcc agcatcaaca gcccgcccag caccgacacc cgcaggccgt
     1501 aggacgccag ggaggcgatc aggacgcggc gcgccggggc atccgccacc agcacctgga
     1561 cggcttggcc gatcatgaag aatgcgatcg tgaccacccc ggccaccgcc gccgacagcg
     1621 cagccgacag tccactcgcc gccgcgaagc ccagcacggc cagcagcagg gcgagatgtc
     1681 cgcccgcagc tcccccgacc agcagctgct gggcccgtct gcggttgagg ctcaattcag
     1741 ccattggcgg ccttcgtcgg agtccgccgt gcccaggtca gatagaccgc gagcccgagc
     1801 gccagcagga aaccgacgaa agtgatcggg tgcgggaaca gaccgatcag caccatgccg
     1861 taggccagca gcgccgacca catccacaac agagccaccg cacgggcgtg gctgtggcca
     1921 aggttcagca tgcggtggtg caagtgcatc ttgtctgcct ggtaccagag cttgccggcc
     1981 agcgtccggc ggacgtacgc cagcaccagg tcgagcagcg gcagcgccat cactgccagc
     2041 ggcagcagga tgggcaggta ggcagcgaac gcattcagcc ccaggccgct cagaccggac
     2101 gggtcgatct ggccggtgag gctgatcatc gcggtggcca gcaggaagcc gagcagcatc
     2161 gccccggagt cacccatgaa catgcgcgcc cggtagatgt tgtagggcag gaagcccagg
     2221 cacaccccaa ccgtcgccac ggtgatcagg ctggacgtgg tggcgcggac gagttcatgt
     2281 tcgtacgaca gcagatatgc gtaagcgaag aacgcaccgg cgccgatcgc caccacgcct
     2341 gcggcgaggc cgtccaggcc gtcgatcagg ttgatcgcgt tcacgcaaag gaagatcagc
     2401 acgacggtca ctgcgatcga agtgccctcg tccagcgaca cgatcgcccc ggggaggggg
     2461 atccagtaga ccttcacgcc gttgagcacc gccaccccgg cggccagcaa ctgtccggcg
     2521 atcttcgcca gcgcgttcag ctcgaacagg tcgtccacca cgccgaccgc gcagatcagt
     2581 cctccggcca gcagcaccac cccagcgtcg tgggtgacca gttcgaagct gcccagccag
     2641 ggcagttggg cggcgagcag gaacgcggcc gcgagcccga accacatcga cacgccgccg
     2701 aagtacggga tcggcttggt gtgcacgtct cggtccctga ccatggcgac ggcgttgtaa
     2761 cggaacgcca gtctgcgcgc gaggccgctg agcaggtagg tcaccccggt ggcgaccagc
     2821 aggacgagca ggtattcgcg catcaggcct gatccgcgtc ctgcggtggc tgttcggcgg
     2881 gatcggacgc cgcctcggcc aggtcgggca ccaccgcgac gagttcggcc cgagtgagct
     2941 tgccttcgcg caggatgcgg ggccgtccgg tcaggtcgac gatcgtggac gggacgccgg
     3001 ccccggccgg gccgccgtcc aggtagaccg acacccgctt gcccagttgc gcctgcgctt
     3061 cggcgacggt gttcgccgca ggctgtccgg agacgttggc cgagctgacc gcgagtgggc
     3121 cggtggcccg cagcagttcg cgggtgaact cgtggtcggg cacccggatc gcgatggtct
     3181 ggccacgatc gcccaggtcc atccggaggt tggcatgcgc ggtgacgacc agcgtgagcg
     3241 cccccggcca gaacgtgtcg gcgagggcga gcatctcctg gctgacctcg tcggccagcg
     3301 agcgcaacgc gtccgcgtcc gcgatcagga ccggtggcgg catgtcgcgg ccgcgttcct
     3361 tggcgtcgag caggccctgc acggcgtgtg ctgagaacgc gtccgcaccg atgccgtaga
     3421 ccgtgtcggt gggcagcacg atgcagttac cggcgctgac ggccgctgcc gccgcctcga
     3481 ttgcaccctc ggggtcagcc gtgatgtccc agaccttcgc catgccgctc atcctgccag
     3541 gttggcggcg cgaactcccg cgacgaaccg tggccgcccg gtcaggtcga ggtggtcggc
     3601 gagctgggtg aacgcgcccc gcacggcgaa cacgtcgagc gcgctgtcgg tctgcacttc
     3661 ggcgtgctcg gcgcacacga agccgcccgg gcgcagcagc cgcgcggcca cctcggcgac
     3721 tacgcgaagg gcgtcgaggc cgtcctcgcc ggagaacagc gccaggtcgg gctcgtgggt
     3781 gcgaacatcc tcggccacgt attcccacgc ggtcagcggg atgtacggcg ggttggcgat
     3841 gaccaggtcg acggcaccgt tcaactccgg gaaggcgtcc gccatgtcgc cttcggtcag
     3901 ggtgacgccg gtgccgctga gattctgctg cgctagcctg gccgcggtct cggagagctc
     3961 caccgcgtac tgcagacagc ccggacgctc gacagcgatc gccttcgaga tcgcgccgct
     4021 gcctgcgcag agttcgacca cgaccggctc gccgggcagc ttcgcaaggc gttcgagcgc
     4081 ccagccagtc agcacttcgg tctccggacg cgggatgaac acacccggcg ccacggccac
     4141 ctcgatcgtg cggaaatagg cgcgaccggt caggtactgc agcggctccc ccgccgccct
     4201 acgggcgatc agctcggcat agtcggcggc tgcggcttca tcgacatcgt cgagcagcaa
     4261 caaccgtgac ggttccacac cggtgacgtg cgcaagcaac gttctagcct ccgcggccga
     4321 gccgagttca gccgtcgccg ccgcgagcag cggcgcactg cggggcatca gcccaccgca
     4381 ccgagacgct cggcgagatc ggcggcctgc agcgcggtga tcaccgcgga caggtcaccg
     4441 gccagcacct ggtcgaggtt gtaggccttg aagccgatgc ggtggtcggc gatccggttc
     4501 tccgggaagt tgtaggtgcg gatccgctcg gaacggtcca ccgtgcgcac ctgcgacttg
     4561 cgggcagcgg acgcggccga ggcggcctca tcctcggccc gggccaccag tcgggcccgt
     4621 agcatgcgca tcgcggactc cttgttctgc aactgcgagc gctcgttctg gcaggacacc
     4681 acgatgccgc tgggcaggtg ggtgattcgg acggccgagt cggtggtgtt cacgccctgc
     4741 ccacccggcc cggaagagcg gtagacgtcg acccgcaggt cctcgtcgtt gatctccacc
     4801 tcggctgcct cgacgtcggg catcaccagc acgccggccg cggaggtgtg cacccggccc
     4861 tgggattcgg tgaccggcac ccgttgcacc cggtggacgc caccctcgaa cttcaaaacg
     4921 ccatagggag cgttctccgg atcgccgttt ccaggagcct tcaccgcgac cgtgatcgac
     4981 ttgtagccgc ccagatcggt gtcctgggaa tcgagcacct cggtcttcca gcccctggtc
     5041 tcggcatatc tggcgtacat ccggaacagg tcgccggcga acagcgccga ctcctcgccg
     5101 ccctcgcccg acttgatctc gatcaggcag tcggccgagt cattggggtc gcgaggcgca
     5161 agcaggcgag tcagcttctc ttccactgtg accagttcgg ccgcgatcgc gtccgcctct
     5221 tcggcgaacg acgggtcctc ggcggccagt tccttcgccg cgaccaagtc actgctgagc
     5281 tgctcgtgcg cgtccaggct gcggacaatc gggctgagct cgttgtagcg tctgccgacg
     5341 cgccgggacg ccgccaggtc ggagtgcagc gctgggtcgg cgagctgccg ttccaggtcc
     5401 gcgtactcct cgcgcaggcc ggctgcggct tcgaacatgg caatcccttc gacgaccggg
     5461 caaacacgaa cgccggggct caggcaatcc tgagcacccg gcgttcggtg aagctacttc
     5521 ttcttggcgt agcgcttctc gaaccgagcc acgcggccgc cggtgtcgag gatcttctgc
     5581 ttgccggtgt agaacgggtg gcaggccgag cagacttcgg cgtgcatgac gccgttcttc
     5641 gcggtgctgc gggtggtgaa ggtgttgccg caggtgcagg tcacctgggt ctccacgtaa
     5701 tcgggatgaa tgcctgactt catttcttct cctgttcagg gcaccgggtc gctctgggtt
     5761 tgggcggctg gattggcctg ctggcgtgaa ccggcaccga ccctccattg tgcctgatcg
     5821 aagcgcaggt gccaaacccg gaaccgcgcc ggtcggcatc gggcacagtg gtgcccatga
     5881 ccgcacttcc tggcaatctc cgcctgctcg gtctcggccc cgagcgcctc cgcgacgtca
     5941 tcgaccttga ccgctgggcg ttcccgtcca gcgatgacct ggcggagacc ctggccctgc
     6001 cgagcccgtt gccctggccg cgcacgttcg gggtggcgga cgcggatgcg gacgaccggc
     6061 tggtggcgat gcacgcgtcc tatccgttcc ggacctgccc ggtgccgggc ggacggctgc
     6121 cggtggccgg actcacctgg gtggccgtgc accccgaggc acggcgtcgc ggcatcctgc
     6181 ggtcgatgat cggggcgcac ctcgcgcatt gccggacgtg gggtgagccg atctcggtgc
     6241 tcaccgcgtc tgaacccgcg atctacggac gcttcggcta cggtctggcc gcccggcagg
     6301 ttgaggtgag gctgccgcgc ggcgcagcgc tgcgtcccgt gcctgggggc gaggcgctca
     6361 ccgtgcgcat cgagcacctc caggtcgagc agcatcggga gctgatcggc cggctgcacg
     6421 gtgcggtcac ccgtccgggt tgggttggcc gggagacgcc ggagttgacc accgcctggt
     6481 tcgcggactc ccccatgttc catcccgggt tcgaatcggc ccgaatcatg atcgccgaac
     6541 gggccggcgt gccggtcggc tacgcggtgt tccggcgcaa cagctcctgg ggaccgggcg
     6601 gtccgacggg cacggtcagg gtgagtgact tcgtcggcgc cgacccgtca gccacccacc
     6661 tgctgtggtc gcggctgctc gacctggacc tcaccaccga agtggtcgtc agcatgctgg
     6721 ccacggacga tccgttgctg agcctgctgg tggacgtgcg cgcggtggta ccgacggtca
     6781 aggacaacct ctggctgcgc ctggtggacg ttgccgccgc cctggccgga cgtcagtacg
     6841 cagctgatct cgatgtggtc ttggaggtat cggacgcgat gctgcccagc aatgccggcc
     6901 ggtggcggct gaccgcgtcc gcgctcggtt cggctgcggt cacccgcacc gacgccgaag
     6961 ccgatctggc gctggacgtc cgtgaactcg gctccgccta cctcggcgga
//
LOCUS       DAOPRQ010000104         7004 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_8909_length_7004_cov_12.805727, whole genome shotgun sequence.
ACCESSION   DAOPRQ010000104 DAOPRQ010000000
VERSION     DAOPRQ010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 7004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7004
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8909_length_7004_cov_12.805727"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            134..1717
                     /locus_tag="PKJ61_11135"
     CDS             134..1717
                     /locus_tag="PKJ61_11135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14415.1"
                     /translation="MPNPVQAAWGRLRPAFTAPWSGNHRLGLGLIIASALVTIAVAFC
                     GPSSVTLKLGERINFLPPWYLPVKWLPLGEWVAVPALWTGIALGALGLLICWRAVNLG
                     WRPSNRRLFLTGSVLSIATSLVPPLTSADVLMYAAYGRIQVLGLNPYDITPAEIFRQE
                     FDPLLIVTERPWQDTPSVYGPLASASQWLAAYLGGGNMHNTVFWLQMFALLPFLLIGA
                     LAVRMAHDDHRTQTRAVLFTVLNPLMIWSVLAGAHNEAFTLVFAIVSLYFMRRSSFVA
                     GLGIGLAGTVKVSLVFYGIAMAWGYRRDWRKLVGLALGAAIPLVIAYGFIVPRALLAA
                     GRNTGYISAGSWAPWLQWAMGWVIGDWAARGVIGWLGWVLMAVVAWMLSRVLPWRLVP
                     GVANTLDPRRDPLTVTVRTAAILTTAWLVTSPYTLSWYDLITWVPLGLMAATRLDVLM
                     MWRGMWLSIAYVTGRSVEFSPTMQAVSVFLRDWLCTGAQWMVLILIVHWWWTSAQRFP
                     AVPAWVPRPGRGRLEAAPH"
     gene            1727..2641
                     /gene="pheA"
                     /locus_tag="PKJ61_11140"
     CDS             1727..2641
                     /gene="pheA"
                     /locus_tag="PKJ61_11140"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004809749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HOC14416.1"
                     /translation="MLGYFGPAGTFTHQALLTISDAEAVPYATVGQAVAAVRSGEIEA
                     ALVPIENSVEGGVSATLDILASGRRLVITKEVLLPVQFNLYARPGTTADQVRRVLTHP
                     HSAAQTRGWMASNLPEAVVTEGGSNAASAQEVADPESRYDAAICGQIAGEMYGLVPVA
                     TEIADNASAVTRFVLVSRPTEPTQPTGADKTTLVAYMHTDRAGALLEILDQFSNRGVS
                     LSRIESRPTKTGLGNYCFSIDAEGHMREPRLAEAMLGLHRICAEVVFLGSYPRADGKR
                     TQVASGFTDADFAAAREWYATLVHPEDD"
     gene            2758..4026
                     /gene="serS"
                     /locus_tag="PKJ61_11145"
     CDS             2758..4026
                     /gene="serS"
                     /locus_tag="PKJ61_11145"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002528250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOC14417.1"
                     /translation="MIDPKLLRTDPDLLRRAQEARGESVELVDELVSADSSRRAAIAA
                     FENLRAEQKELGKQVARAQGEEKAAVLARTRELAAEVKAAQAASDEAGAKFDELMARL
                     PNPVFEDVPRGGEDDFVVLETIGAPRDFAAEGFEPRDHLELGEMLGAIDMERGAKVSG
                     SRFYFLTGVGARLELALINLALTKAMGWGFTPILPPALVKPSAMEGTGFLGQAAQDVY
                     HLPADDLYLVGTSEVALAAYHSEEILDAATLPRHYVGYSPCFRREAGSYGKDTRGIFR
                     VHWFDKVEMFVYCDPAEAQAEHQKLLGFEKDFITALEIPFQVLEVASGDLGLSAARKY
                     DCYGWLPTQGKYREITSTSNCTEFQARRLNIRMRDADGVRSVATLNGTLCAMTRMIIM
                     LLENHQQADGSIYVPAALRPFLGGAEYLRP"
     gene            4232..>5034
                     /locus_tag="PKJ61_11150"
     CDS             4232..>5034
                     /locus_tag="PKJ61_11150"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOC14418.1"
                     /translation="MNSHNSAAWRPQVVALDIDGTLVDHAGVLPNEVRRAVRRVTAAG
                     VPVVLTTGRSWHATRPVFEQLGLPEGPAISSNGAVIVQFPPFELTQVVTFDPSAVVER
                     VLQEHPAAALAAEVVGAGYRVTKHFPEGDLSGEIEVVSVEDLAGSDVTRIVVRDPEAS
                     DGEFIALAQRLGLHGVSYSVGWSAWLDIAPAGVNKATALADVVADLGVAAADVLAIGD
                     GRNDIEMLTWAGRGVALGDACPEVHLVADHVTGRFAEGGTAAELNRWFG"
     assembly_gap    5035..5134
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5188..6522
                     /locus_tag="PKJ61_11155"
     CDS             5188..6522
                     /locus_tag="PKJ61_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4921 family protein"
                     /protein_id="HOC14419.1"
                     /translation="MQPFIREPEFLTQLKDGTVKQLNPFSGTEVWTVAGRGNRPLGIT
                     LPDPVPLDPAKEGHHCPFCEQRYLETPPEKSRVIRHGDGNWETRYRVAAEDLHATVAE
                     FRRIPNLFEILSFDYWNLNYGYELPSRVRERKAAYLDSAAGRDHVLAVVRNKLKAGGR
                     TDEMIDALGEDGQLAAASGFFGGGHDVIVARRHFVDGATDNTQLAASGTLTPEEHYRF
                     FAFTIESTQALFDLNRYARYVAVFQNWLKPAGASFDHLHKQLVAIDERGVQHELAMDR
                     LRTNPNIYNEYGVNYASYHNLVLAENDHAVAFAGFGHRYPTIEIYSKSEACDPWNHTP
                     EEIRGVSDLVHAMHAASGVAVPSNEEWHYRPIDTDLAMPWRVMLKWRNSTIAGFEGAT
                     KIYLNTIDPYALRDRLVPALFALREQRRLGDLRIATECPCRPNTLRYRTNRS"
     gene            6550..>7004
                     /locus_tag="PKJ61_11160"
     CDS             6550..>7004
                     /locus_tag="PKJ61_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOC14420.1"
                     /translation="MIPSYELNDGHRLPVIGFGTYGLWGADGAEAIASGLRAGYRLID
                     SAVNYENEGAVAEGIRRSGVPAEEIIITSKLPGRHHAYAKARTCVYESLYRLRTDQIG
                     LYLIHWPNPLEDRFVEAWQALIDLQSEGVLRSIGVSNFLPEHLSRIIGET"
BASE COUNT         1067 a         2302 c         2351 g         1184 t
ORIGIN      
        1 cgggtatccc gccaccctag gcgtgagctt gccgtccgct tcgtaacggt ccggtctcgg
       61 ccgcggtcgg acgtgggttg ccctgtgccg tacaggtagc ctgctagatg gtccatccga
      121 tcgtgaggtg agaatgccga atccggttca ggccgcctgg gggcggctgc gtccggcatt
      181 cacggcaccc tggagcggca accatcggct cggtctcggg ttgatcatcg ccagtgcgct
      241 ggtcacgatc gcggtcgcgt tctgcgggcc gtcctcggtc acgctgaagc tgggcgagcg
      301 catcaacttc ctcccgccct ggtacctccc ggtgaaatgg ctgccgctgg gcgaatgggt
      361 ggccgtccct gcgctgtgga cggggatcgc gctcggcgcg ctcggcctgc tgatttgctg
      421 gcgcgcggtg aacctcggct ggcgtccgag caaccgccgg ctgttcctca ccggctcggt
      481 cctcagcatc gccaccagtc tggtgccacc gctgacttcg gcggacgtgc tgatgtacgc
      541 cgcctacgga cgcatccagg tgctcggcct gaacccctac gacatcactc ccgcggaaat
      601 cttccggcag gagttcgatc cgctgttgat cgtcaccgag cggccatggc aggacacgcc
      661 gagcgtctat gggccgctcg cgtccgcgtc ccagtggctg gccgcctacc tcggcggcgg
      721 caacatgcac aacacggtct tctggctgca gatgttcgcg ctgctcccgt tcctgctgat
      781 cggcgcgctc gccgtccgga tggcgcacga cgaccatcgg acgcaaaccc gcgccgtgct
      841 gttcaccgtg ctgaacccgc tgatgatctg gtcggtgctg gccggtgcgc acaacgaggc
      901 gttcaccctg gtgttcgcga tcgtcagtct gtacttcatg cggcgcagct cgttcgtggc
      961 cgggttgggc atcgggcttg ctgggactgt gaaggtttcg ctggtgttct acggcatcgc
     1021 catggcctgg gggtaccggc gggactggcg caagctggtc ggactggcgc tcggtgccgc
     1081 gattccgttg gtgatcgcat acggcttcat cgtgccgcgg gcgctgctgg ccgctggccg
     1141 caacaccggc tacatctccg ccggctcctg ggcgccgtgg ctgcagtggg cgatggggtg
     1201 ggtgatcggc gactgggccg cgcgcggcgt gatcggctgg ctgggttggg tgctgatggc
     1261 ggtggtcgca tggatgctct cgcgggtact gccctggcgg ctcgttcccg gggtggccaa
     1321 cacgctcgat ccgcgccgcg acccgctcac cgtcaccgtg cggacggccg cgatcctcac
     1381 caccgcctgg ctggtcacgt ccccctacac gctgagttgg tacgacctga tcacctgggt
     1441 gccgctgggg ttgatggccg ccaccaggct ggacgtgctg atgatgtggc gtggcatgtg
     1501 gctgtcgatc gcctatgtaa ccggccgctc ggtggagttc agcccgacta tgcaggcggt
     1561 gtcggtgttc ctccgtgact ggttgtgcac cggtgcccag tggatggtgc tgatcctgat
     1621 cgtccactgg tggtggacat cggcgcagcg cttccctgcg gtgcccgcct gggtgccgcg
     1681 cccgggacgc ggccggctcg aagccgcacc ccactaggct gagcgggtgc tcggatactt
     1741 tggccccgca gggacgttca cgcatcaggc tctgctgacg atctcggacg ccgaagctgt
     1801 gccgtacgcg accgtgggtc aagcggtggc cgccgtccgc tccggtgaga tcgaggcggc
     1861 tctggtgccg atcgagaact ccgtggaggg tggggtttcg gccacgctcg acatcctcgc
     1921 gtccgggcgg cggctggtga tcaccaagga agtgctgctg ccggtgcaat tcaacctgta
     1981 cgcacgcccg ggcaccacgg ccgaccaggt gcggcgggtg ctcacccacc cgcattcggc
     2041 cgcgcagacc cgaggctgga tggcttcgaa cttgcccgag gcagtggtca ccgagggtgg
     2101 gtcgaacgcc gcgtccgcgc aggaggtggc cgacccggag tcgcgctacg acgccgcgat
     2161 ctgcggccag atcgccggcg agatgtacgg cctggtgccg gtggccacgg agatcgccga
     2221 caatgcgtcc gcggtgacga ggttcgtgct ggtgtcgcgt ccgaccgagc cgacccaacc
     2281 gacgggtgcc gacaagacca cgctggtggc gtacatgcac accgaccggg ccggcgcgct
     2341 gctggagatc ctcgaccagt tctcgaaccg cggagtgagc ctcagccgga tcgagtcgcg
     2401 tccgaccaag accggcctgg gcaactactg cttcagcatt gacgccgagg gccacatgcg
     2461 cgagccgcgg ttggctgagg cgatgctcgg actgcatcgg atctgcgctg aggtggtgtt
     2521 cctcggctcg tatccgcggg cggacgggaa gcgcacccag gtggccagcg gcttcaccga
     2581 cgccgacttc gccgccgcca gggagtggta cgccaccctg gtgcaccccg aggacgactg
     2641 agctgaacgg attcgcactc gatcagcctt tgcgatggcg gttcgagggt ttccacgcct
     2701 agttgcgcgc aagtccgttc gaggtcgccc gatgggtgtc cgataggctc ttggcccgtg
     2761 atcgacccga agctgctgcg taccgaccct gacctgctcc gccgcgccca ggaggcccgc
     2821 ggcgagtcgg tcgaactcgt cgacgaactg gtcagcgcgg attcgtcccg gcgcgctgcg
     2881 atcgctgcct tcgaaaacct gcgggccgag cagaaggagc tcggcaagca ggtggcccgc
     2941 gcccaggggg aagagaaggc cgcggttctc gcgcgcacca gggagctggc ggcagaggtg
     3001 aaggctgccc aggcagcgtc cgacgaagcc ggtgcgaagt tcgacgagct tatggcccgg
     3061 ctgcccaacc cggtgttcga ggacgtcccg cgcggtggcg aggacgactt cgtggtgctg
     3121 gaaaccatcg gtgcgccgcg cgacttcgcc gccgagggct tcgagccgcg tgaccacctc
     3181 gaactcggcg agatgctcgg cgcaatcgac atggagcggg gcgcgaaggt ctccgggtcg
     3241 cggttctatt tccttaccgg cgtcggtgct cggctggagt tggcgctgat caacctggcg
     3301 ctgaccaagg cgatggggtg gggcttcacc ccgatcctgc cgccggcgct ggtcaaaccg
     3361 tccgcgatgg agggcaccgg cttcctcggc caggccgcgc aggacgtgta ccacctgccc
     3421 gccgacgacc tatacctggt cggcacctcc gaggtggccc tggccgccta ccactccgag
     3481 gagatcctgg acgcggccac gctgccgcgg cactacgtgg ggtacagccc gtgcttccgc
     3541 cgcgaggccg gttcgtacgg caaggacacc cgtggcatct tccgggtgca ctggttcgac
     3601 aaggtcgaga tgttcgtcta ctgcgacccg gccgaggccc aggccgaaca ccaaaagctg
     3661 ctcggcttcg agaaggattt catcactgcg ctggagattc cgttccaggt gctggaggtg
     3721 gcctccggtg acctcggcct gagcgccgcc cgcaagtacg actgctacgg ctggctgccc
     3781 acccagggca agtaccgcga gatcacgtcc acctcgaact gcaccgagtt ccaggcccgc
     3841 cggctgaaca tccgcatgcg cgacgcggac ggcgtccgct cggtcgccac gcttaacggc
     3901 accctctgcg cgatgacccg gatgatcatc atgctgctgg agaaccacca gcaggccgac
     3961 ggctcgatct acgtcccggc cgccctgcgt cccttcctgg gcggcgcgga gtacctgcgc
     4021 ccctgagtgg gccactggtc tttcccgctg acgttgccgc cggtcctggc ggcgcgaaca
     4081 cgccggcgaa agtgacatcc gccggggaat gttcgccggc aactgtccgg atcgctggcg
     4141 gcaatcagcg ggacgggctt ggggggccga cgtgaggcgg acgcccggcg ttcaccgggt
     4201 gttcggaatg gtgagctagc ctggtttgaa gatgaatagt cacaacagcg cggcttggag
     4261 gccgcaagtg gtggcactcg atatcgacgg gacgctcgtc gaccacgcgg gagtgttgcc
     4321 aaacgaggta cgccgggcag tccggcgagt cacggcggcc ggggttccgg tcgtcctaac
     4381 gacgggacgc agttggcacg cgacgcgtcc ggtcttcgaa cagcttggcc tgccagaggg
     4441 gccggccatt tcgtcaaacg gcgcggtgat cgtgcagttc ccgccgttcg agctcaccca
     4501 ggtggtcacg ttcgacccgt ccgcggtggt ggagcgggtg ctgcaggagc atccggcagc
     4561 ggcgctggcc gccgaggtgg tgggcgccgg ctatcgcgtc accaagcatt ttcccgaagg
     4621 ggacttgagc ggcgaaatcg aggtggtcag cgtcgaagac ctggccggca gcgatgtcac
     4681 cagaatcgtg gtgcgcgacc cggaggcgtc tgatggtgag ttcatcgcct tggcgcagcg
     4741 gctcggcctg cacggcgtga gctactcggt gggctggagc gcctggttgg atatcgcacc
     4801 ggcgggggtc aacaaggcca ccgcactggc ggacgtggtc gccgacctcg gcgtcgccgc
     4861 tgcggacgtg ttggccatcg gcgatggccg caacgacatc gagatgctca cctgggccgg
     4921 acgcggggtc gcgctgggcg acgcctgtcc cgaagtccac ctggtcgccg accatgtcac
     4981 cggacggttc gccgaaggcg gcaccgcggc ggagctgaac cgctggttcg gcgannnnnn
     5041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnaacgga ctagtctgaa tcggtccaaa
     5161 gacgcggacg cggcgtgagg ggtatcgatg cagccattca ttcgggagcc ggagttccta
     5221 acccagctca aggacggcac ggtcaagcag ctcaacccgt tcagcggcac cgaggtgtgg
     5281 acggtcgcgg gccgcggtaa ccgtccgctg ggcatcactc tgcccgaccc ggtgccgctc
     5341 gaccccgcca aagagggtca tcactgcccc ttctgtgagc agcgttacct tgagacgccg
     5401 ccggagaagt cccgggtgat tcgccacggc gacggcaatt gggagactcg ctaccgggtt
     5461 gccgccgagg acctgcacgc cacggtcgcc gagttccggc gcatcccaaa cctcttcgag
     5521 atcctttcct tcgactactg gaacctcaac tacggctacg aattgcctag tcgcgtccgt
     5581 gagcgtaaag ccgcctacct cgattccgcg gccggtcgcg atcatgtcct cgccgtcgtc
     5641 cgcaacaagc tcaaagccgg cggacgtacc gacgagatga tcgatgcgct cggagaggac
     5701 ggccagctcg cggctgcgtc cggttttttt ggcggtgggc acgacgtgat cgtcgcccgc
     5761 aggcacttcg tggacggcgc caccgacaac acccaactgg ctgcgtccgg cacgctcacg
     5821 cccgaggagc actaccggtt cttcgctttc acgattgaat ccacccaggc gctgttcgac
     5881 ctcaaccgct atgcccgcta cgtcgcggtc ttccagaact ggctgaagcc cgcgggcgcg
     5941 tccttcgacc acctgcacaa gcagctggtc gcgatcgacg aacgcggggt gcagcatgaa
     6001 ctcgccatgg atcggctgcg caccaacccg aatatctaca acgaatacgg ggtcaactac
     6061 gcctcgtacc acaacctcgt gctcgccgag aacgaccatg cggtggcctt cgccggcttc
     6121 gggcaccgct acccgacgat cgagatctac tccaagtcgg aagcctgcga tccctggaac
     6181 cacacgcctg aggaaatccg cggcgtctcc gacctggtac acgccatgca tgccgcgtcc
     6241 ggggtggcgg tgccgtcgaa cgaggagtgg cactaccgtc cgatcgacac cgacctggct
     6301 atgccgtggc gcgtgatgct gaaatggcgc aattcgacca tcgccggctt cgaaggcgca
     6361 accaagatct acctcaacac gatcgacccc tacgccctgc gtgatcgcct ggtgcccgcg
     6421 ttgttcgccc tgcgggagca gcgccggctc ggcgacctgc ggatcgccac cgagtgcccg
     6481 tgtcggccca acaccctgcg atatcggacg aaccgaagct gagccgggcg ccggggtaac
     6541 gtctggggca tgatccccag ctacgaactc aacgacggcc accggcttcc cgtgatcggt
     6601 ttcggcacct acggcctctg gggcgcggac ggggccgagg ccatcgcgtc cgggctgcgc
     6661 gccggctatc ggctcatcga ttcggcggtg aactacgaga acgagggtgc ggtcgccgag
     6721 ggcatccggc gctccggggt gccagccgag gaaatcatca tcacctccaa gctgcccggg
     6781 cgccaccacg cctacgcgaa ggccaggacc tgcgtctacg agtcgctcta ccggctgcgc
     6841 accgaccaga tcgggctgta tctcattcac tggccgaacc cgctcgagga ccgctttgtc
     6901 gaggcttggc aggcgctgat cgacctgcag tcagagggcg tgctgcgctc catcggggtg
     6961 agcaattttc tccccgagca cctcagccgg atcatcggcg agac
//
LOCUS       DAOPRQ010000105         6889 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_9116_length_6889_cov_12.524729, whole genome shotgun sequence.
ACCESSION   DAOPRQ010000105 DAOPRQ010000000
VERSION     DAOPRQ010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 6889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6889
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9116_length_6889_cov_12.524729"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..179)
                     /locus_tag="PKJ61_11165"
     CDS             complement(<1..179)
                     /locus_tag="PKJ61_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HOC14421.1"
                     /translation="MFDRFTDRARRVIVLAQDEARMLNHNYIGTEHLLLGLIHEGEGV
                     AAKALESLGISLEAVR"
     gene            complement(339..674)
                     /locus_tag="PKJ61_11170"
     CDS             complement(339..674)
                     /locus_tag="PKJ61_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lsr2 family protein"
                     /protein_id="HOC14422.1"
                     /translation="MAKRVQVIHVDDIDGSDAAETIAFALDGINYSIDLSAGNAAKLR
                     ESFAPYIAAGERQRGASNRRVPATGRRTGGGTAATAIRAWATEQGMQVSARGRVSAEV
                     REAYERAHS"
     gene            complement(917..1501)
                     /locus_tag="PKJ61_11175"
     CDS             complement(917..1501)
                     /locus_tag="PKJ61_11175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14423.1"
                     /translation="MKTSATHRFASSSPLRRNAWISLAVSSVVAVACAFGTLWVTRAG
                     IAVAIVGGLLAAKFAWQEIRASRNALLAQNSADARDAATLLHTERAQHVRLLGVLQLR
                     NADLRSKLNTTRAEAAVLSQEAAQLRGDKAALELELAKQQESAQAQVFPWPRRVTGHG
                     EAQDIWVSDGAPTVVELQALANPPLSSAPQRQHA"
     gene            complement(1533..2033)
                     /locus_tag="PKJ61_11180"
     CDS             complement(1533..2033)
                     /locus_tag="PKJ61_11180"
                     /inference="COORDINATES: protein motif:HMM:NF022819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3180 family protein"
                     /protein_id="HOC14424.1"
                     /translation="MNDAPPPRPTVAATSWQAVAAAALLGGGCGWLLFAGLDALGMPA
                     PQLPIVVTFAIALFALLVGVQAVRTHRLVQVRREELPARKAVALLVLGKTCVLAGAGL
                     AAGYAAVAAYFWSRQDAMLPRQRVLSSVVAVVVSVALAVAGAFLERACRIPRTTDEDA
                     TPPSER"
     gene            complement(2030..2536)
                     /gene="folK"
                     /locus_tag="PKJ61_11185"
     CDS             complement(2030..2536)
                     /gene="folK"
                     /locus_tag="PKJ61_11185"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007078951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HOC14425.1"
                     /translation="MRQPRNSVFSIGSNLGDRMEYLNLAVRALRATPGLRLTGVSSVY
                     ATAPVGVVTQPDFLNAVVAVESTLPSMVMLERALAIEDALNRVRTTRWGPRTIDVDLI
                     TVDDRQLESPELTLPHPRAHERAFVLVPWLEVQPDAVLVGHGPIVDLVAGLDTAGVRR
                     CDDLRIDW"
     gene            complement(2565..2933)
                     /gene="folB"
                     /locus_tag="PKJ61_11190"
     CDS             complement(2565..2933)
                     /gene="folB"
                     /locus_tag="PKJ61_11190"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020579449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HOC14426.1"
                     /translation="MLPDRITLTGIQVSARHGVYAAEKVTPQLFLIDVTCSLAAPASA
                     DDLATTVDYAELSEQIADVATTGSVDLIETLAERVAAVCLAWALVAEVEVTVHKPQAS
                     MPVAVADIAVTITRRRASDR"
     gene            complement(2956..3756)
                     /gene="folP"
                     /locus_tag="PKJ61_11195"
     CDS             complement(2956..3756)
                     /gene="folP"
                     /locus_tag="PKJ61_11195"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOC14427.1"
                     /translation="MGILNVTPDSFSDGGEFLDTDAAVRHGLALAAEGADLVDVGGES
                     TRPGALRAEPDVELARVVPVIERLVAAGVNVSIDTMRVTVAEAAVRAGAVLVNDVSGG
                     LADGQMLEAVAELGVDYVAMHWRGPSDHMTELAHYGDVVTDVVAELHDRLAAATRAGV
                     RRTRIILDPGLGFAKNAEHNWALLSRIDELAALGCPLLVGASRKRFLGQLLATADGPR
                     PVREREDAGVAITALLAVHGVWGVRTHTVRAHRDAIEVAEQLRVGGSR"
     gene            complement(3827..4393)
                     /gene="folE"
                     /locus_tag="PKJ61_11200"
     CDS             complement(3827..4393)
                     /gene="folE"
                     /locus_tag="PKJ61_11200"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002549559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HOC14428.1"
                     /translation="MRVAVDQERVAAAVREILLGIGEDPDRDGLRKTPERVARAYAEM
                     FDGLDNDPAEILSTTFDLGHDELVLVRDIEVWSTCEHHLLPFTGVAHVGYIPTKGRIT
                     GLSKLARLVDAFAKRPQVQERLTSEVADALVQHLQPRGVIVVIECEHLCMTMRGVRKP
                     GAKTVTSAVRGILANSATRAEAMSLIIG"
     gene            complement(4732..6642)
                     /gene="ftsH"
                     /locus_tag="PKJ61_11205"
                     /pseudo
     CDS             complement(4732..6642)
                     /gene="ftsH"
                     /locus_tag="PKJ61_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002517215.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
BASE COUNT         1110 a         2382 c         2299 g         1098 t
ORIGIN      
        1 cggacggctt ccagggagat gcccagcgac tccaacgcct tggcggcgac gccctcaccc
       61 tcgtgaatga gcccaagcag caggtgctcg gtgccgatgt agttgtgatt gagcatgcgc
      121 gcctcgtcct gggcgagcac gatcacacgt cgagcacggt cggtgaacct atcgaacatg
      181 cttggaactc cttatccaac tgcggcaggc gggcgtccgc tcaacgcgga gcccggcccc
      241 ttcactttag tcaacgcagc cgcatgcccg ggcagtcccg tgttcgcccc cggctcaatc
      301 cgatcctccc ggggccgata ccaacctcaa ggagccgatc agctgtgcgc ccgctcgtat
      361 gcctcgcgaa cctcggcgga aacccggccg cgagccgaga cctgcatgcc ctgctcggtc
      421 gcccaggccc ggatcgcggt ggctgcggtg cctccgcccg tcctgcgacc ggtcgccggc
      481 acgcgccgat tgctcgcacc ccgctggcgc tcgcccgccg cgatgtaggg cgcaaacgat
      541 tcacgcagct tcgcagcgtt tcccgcagac aggtcgatgc tgtagttgat tccgtccaat
      601 gcgaaggcga ttgtctcggc agcatcactg ccgtcgatgt cgtcgacgtg aatcacctga
      661 acacgctttg ccacttcgtt cccctcaagt ccagtgcaga cagcttcccc aatccctcgc
      721 gagcatatac cgacaatccc aagaacggcc tagctgaatt cgttccgaga tagcatttcg
      781 gcgacaatcc gtacgaactg gttcaccgaa agcaactgat ccgtcaaaac agttgatgaa
      841 tgcaggtcct cgacgctggg gataagaccg gccgccgtcg tgcgcccggc ccggggttcg
      901 acagaagcgt gccggctcag gcgtgctgac gctgcggcgc cgaagacagc ggcggattcg
      961 ccagggcctg caattccacg acggtgggcg cgccgtcgga gacccagatg tcttgagcct
     1021 caccgtgacc ggtcacccgg cgcggccagg ggaacacctg agcctgcgcc gactcctgct
     1081 gcttggcgag ttccagttcc aatgcggcct tgtcgccgcg aagctgggcc gcctcctggc
     1141 tgagtaccgc ggcttcggcc cgagtggtgt tgagcttgct ccggaggtcg gcattgcgca
     1201 gctgcaggac cccgagcagg cggacgtgct gggcgcgctc ggtgtgcagc aacgtcgccg
     1261 cgtcgcgagc gtccgcgctg ttctgggcga gcagcgcgtt acgggacgcg cggatctcct
     1321 gccaggcgaa cttagcggcg agcagcccac ccacgatggc cacggcgatg ccggcacggg
     1381 tcacccagag cgtgccgaag gcacaggcga ccgccacgac cgaactcacc gccagcgaaa
     1441 tccaggcgtt gcggcgcagc ggggaggaac tcgcgaatcg gtgcgtggcg ctggtcttca
     1501 cagcccggag tctagtcaag cagttgccga cttcagcgct ccgacggcgg cgtggcgtcc
     1561 tcatctgtgg tacgcgggat ccggcaggcg cgctcgagga aggcgccagc caccgcgagc
     1621 gcgacgctga ccacgaccgc caccaccgaa ctgagcacgc gttgccgcgg caacatcgcg
     1681 tcctggcggc tccagaagta ggcagccacc gcggcgtagc cggcagccag gccggcgccg
     1741 gcgagcacgc aggtcttgcc gaggacgagc aacgccactg ccttgcgggc gggcagctcc
     1801 tcacggcgca cctgcaccag gcgatgcgta cggaccgcct gcaccccaac cagcaacgcg
     1861 aacagcgcga tcgcgaacgt caccacgatg ggcagctgcg gcgcgggcat cccgagcgcg
     1921 tccagcccgg cgaacagcag ccagccacag ccgccaccga gcagtgcagc cgcggcgacc
     1981 gcctgccagc tcgtggccgc aacggtgggc cgcggcggtg gcgcgtcgtt caccagtcga
     2041 tcctcagatc gtcgcagcgg cggacgccgg cggtgtccag gccggccacg aggtcgacga
     2101 ttgggccgtg cccgaccagg actgcgtctg gctgcacctc gagccacggc accagaacga
     2161 aggcacgctc gtgggctcgc gggtgcggca acgtcagctc cggcgattcg agttggcgat
     2221 catcgaccgt aatgaggtcg acgtcgatgg tgcgcggccc ccagcgggtg gtgcggacgc
     2281 gattgagcgc atcttcaatg gccaaggcac gttcgagcat gaccattgac ggcagcgtcg
     2341 attcgactgc gaccactgca ttaaggaaat ctggttgggt caccacccct accggagcgg
     2401 tcgcatagac cgaggaaacg ccggtcagcc gtaaccctgg agtagcccga agggcgcgta
     2461 ccgccaagtt gaggtattcc attcggtcgc caaggttcga gccgatggag aacaccgagt
     2521 tccgcggctg ccgcatttcc gcgcgcatag cattacctgc cggattagcg gtcactggct
     2581 ctcctgcggg tgatggtcac ggcgatgtca gccaccgcga ccggcattga tgcctgcggt
     2641 ttgtgcacgg tcacctccac ctcggcgacc aacgcccagg ccaggcagac ggcggccacc
     2701 ctctcggcta gggtctcgat gagatcgacc gagccggtgg tggcgacgtc cgcgatctgc
     2761 tcggacagct cggcgtagtc gacggtggtg gcgagatcgt ccgccgatgc cggtgcggcg
     2821 aggctgcagg tcacgtcgat caggaacagc tggggcgtga ccttctccgc ggcatagacg
     2881 ccgtgccgag cggacacctg aatgccggta agggtgattc ggtcgggcaa catcagtcct
     2941 ccttcgcagc cgaggttacc gactgccgcc gacgcgcagc tgttcggcga cttcgatcgc
     3001 gtcgcggtgg gcccggacgg tgtgcgtccg tacgccccag acaccgtgta cggcgagcag
     3061 ggcggtgatc gcaacgcccg cgtcctcccg ttcgcggacc ggacgcggac cgtccgcggt
     3121 ggctaggagt tggccgagga aacgcttgcg ggaagcgccg accagcagcg ggcaaccaag
     3181 cgcggccaac tcgtcgatgc ggctgagcag ggcccagttg tgctcggcat tcttcgcgaa
     3241 gccgagtcca gggtcgagga tgatccgggt gcgccggaca ccggccctcg tggccgcggc
     3301 gaggcggtcg tggagttcgg cgacgacatc ggtgaccacg tcgccgtaat gggccagctc
     3361 ggtcatgtga tcggacggac cgcgccagtg catggcgaca tagtcgaccc cgagctcggc
     3421 cactgcttcg agcatctgcc cgtcggccag tccgccggag acatcgttca ccagcaccgc
     3481 tccggcgcgg acggcagcct cggccaccgt cacgcgcata gtgtcgatcg acacgttcac
     3541 cccggccgcg accagtcgtt cgatcaccgg gacgacccgg gccagttcga cgtccggctc
     3601 cgcccgcaga gcgcccggcc gggtcgactc accgcccacg tccaccaggt cggcaccctc
     3661 ggccgccaac gccagcccgt gccgtaccgc agcgtcggtg tcgaggaact ccccgccgtc
     3721 ggagaacgaa tccggcgtca cgttcaggat gcccatcacc agcgtgcgtc cgggagtggg
     3781 aagtctgctc actctggaag cgtaatcgct gcgcagtggg gcggcctcag ccgatgatca
     3841 ggctcatcgc ctcggcgcgg gtggccgagt tcgcgaggat gccgcggacg gcgctggtga
     3901 cggtcttggc gccgggcttg cggacgccgc gcatggtcat gcacaggtgt tcgcactcga
     3961 tcaccacgat cacgccgcgc ggttgcaggt gctgcaccag cgcgtccgcc acttcggacg
     4021 tgagtcgctc ctgcacctgt ggacgcttgg cgaacgcgtc caccagccgc gcgagcttgc
     4081 tcagtccggt gatcctgccc ttggtcggga tgtagcccac gtgggccacg ccggtgaacg
     4141 gcagcaggtg gtgctcgcag gtgctccaca cctcgatgtc ccgcaccagc accaactcgt
     4201 cgtgaccgag gtcgaaagtg gtcgaaagaa tttcggccgg atcattgtcc aggccgtcga
     4261 acatctccgc gtaggcgcgt gccacccgtt cgggggtctt gcgcagaccg tcccgatccg
     4321 ggtcctcgcc gatgcccagc aggatctccc ggacggccgc ggccacccgc tcctggtcga
     4381 ccgccaccct cagctccccg gcttgtcctg gcccggctgg accggcgggg ccggttcgcc
     4441 gccagcctcg ggcgcgaacc ccgactcagt cccgctcggg tacggacgcg agtagtcagg
     4501 ctgttgcggg tagcccggct ggccgtacgg cgggtagccc ggctggccat aacccggcgg
     4561 cgggtagccc ggtgcctgcg ggtagaccgg ctgcccgtaa ggttggccga cgggcggctg
     4621 cccctggcca ccgttcggct gaccgtaggc gaactgcggg tcgtaggcca ccggctggcc
     4681 caccggcagc gcacccacgc ccggatccgg agtcaccggc tgcgccggac gctccggcgg
     4741 gtccaccggc ggtaccgagc tcggtttgcg ggtcgacgac ccagtccagg ccggacgctt
     4801 cggccagcgc tgtaggggct cgaagatctt ggcgacctgc tccttatcga gggtctcctt
     4861 ctcgaacagc tggcgcacca gttcgtccag cacctcacgg ttggtctcga ggacgtcgaa
     4921 cgcctcctga tgcgcggtcg cgatcagccg ggacacctcc gcgtccacct tcgcggcgac
     4981 cgactcggag tactcgcggg tcgaccccgc gccgtagctc atgccgagga agggctcgtg
     5041 gtcaccggac cccagcttga ccgcgccgag ttcctcggtc atgccgtact cggtgaccat
     5101 cgcccgggcc agcttggtgg ccttctcgat gtcgttgccg gcaccggtgg tcgggtcgtg
     5161 gaagaccagc tcctcggccg cgcgtccgcc catcatgtag gcgagctggt cgagcagttc
     5221 gccgcgggtc tgggagtact tgtcgtcgtc cggcatcacc atcgtgtagc ccagcgcccg
     5281 tccgcgcggc aggatcgtca ccttctgcac cgggtcggta cccggcatcg cggcggccac
     5341 cagtgcgtgt ccgccctcgt ggtaggcggt gatcagcagt tcacgctcgt tcatcacccg
     5401 gctgcgcttc tgcggtccgc cgatcacgcg gtcgatggcc tcgtccagct gtggcttgcc
     5461 gatgaagcgc aggttcagtc gagcggtcag caacgcggcc tcattcagca cgttggccaa
     5521 gtcggcgccg gtgaagcccg gcgtccgctt ggcgaccatg gccaggtcga cgtccggggc
     5581 gatcggcttg ttcgagccat ggacgcgcag gatctgcagc cggcccttca tgtccggcgc
     5641 ctccaccggg atctgccggt cgaagcgtcc cggacgcagc agggcggggt cgagcacgtc
     5701 cggacggttg gtcgccgcga tcagcaccac gccgccgtgc acgtcgaagc cgtccatctc
     5761 gaccagcaac tggttcaggg tctgctcgcg ctcgtcgtgg ccgccgccca tgcccgcgcc
     5821 gcggtggcgt ccgacggcgt cgatctcgtc gatgaagacg atggccgggg cagcctcctt
     5881 ggcctgctcg aacaggtcgc ggacgcggga agcgcccacg ccgacgaaca tctccacgaa
     5941 gtcggagccg gaaatcgaga agaacggcac cccggcctcg ccggcgaccg cgcgggccag
     6001 cagcgtcttg ccggtgccgg gagggccgta gagcagcacg cccttaggga tcttcgcccc
     6061 gacggcggtg aacttggccg gctcggagag gaactcgcgg atctcctgga gctcctcgat
     6121 cgcctcctca caaccggcga catcgctgaa cgtggtcttg ggcgtgtcct tactcgccag
     6181 cttggccttc gacttgccga agcccatgac gcggttgccg ccgccctgga tgttgttgaa
     6241 catgaagaag aacaggaagc cgatcaccag gaacggcagc accgtgaaca ggaaagtgct
     6301 ccagtagttc ggctgcgccg gctcgccacc ccacttgtcg agggtcccgg cggccacccg
     6361 ggcgttcaga cgctcgatga tgtcctggct ctggtttccc acccagtagg agcggaactt
     6421 ccgcgccggc gtctctttgg tggtgatctt gatcgtctgc tcgccgtcgg tcagcagcac
     6481 ctcagccagc ggctcgtttc cgttgatcat cgtgaccacc tcggaggtgg gcttctcggt
     6541 gtagccgccg acgttcccga cggcctcgaa gatcagcgcg atggccagga agccgagcac
     6601 gatccagagg atgggagatc ggaaaaaccg gtttggtttc attgcctgcc ttcgtcagct
     6661 tcgcgttgcg caactaggac gataccgaag cgggcaacct gagcgcgcag cggccgctcg
     6721 caacccggca gccgccgaac cgtccacgca gtcccaaccc accgatcgcg gagagatccc
     6781 ggcctacgcc gggatgacgg tggagcggga acgggacgac ggtgtggcgg ccaatcagca
     6841 gaaactggac ggatgcggcc caggacgggc caggactgtc cagtttgcg
//
LOCUS       DAOPRQ010000106         6719 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_9410_length_6719_cov_10.292317, whole genome shotgun sequence.
ACCESSION   DAOPRQ010000106 DAOPRQ010000000
VERSION     DAOPRQ010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 6719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6719
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9410_length_6719_cov_10.292317"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(17..1078)
                     /locus_tag="PKJ61_11210"
     CDS             complement(17..1078)
                     /locus_tag="PKJ61_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007078392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOC14429.1"
                     /translation="MSSLAVHAVIRSRGVALDLEVAEGSVVALVGPNGAGKSSLLHLV
                     AGLAQPSSGEVRIGGREVASRDRCLPPHRRRVALLTQRATLFPHLSVLENVAFGPRAA
                     GNSPAAARTRALTELEAVGCAEFASRRPDALSGGQAQRVAIARALATEPEVVLLDEPL
                     AGLDVAVAAEVRHTLAARLRGRTALLVTHEVLDLWTIADQLAVITAGKVVESGPVAEL
                     LTRPASAFLARLGGTNLVAGVAVGADLLEVAPGVMLRGLADPEQPLAVGAAGLASIQP
                     EAISLHLAVPGGSPRNTLAAKVTGIEPRGQVVRVHLAVAGHELLADLTAMSVAELGLR
                     PGAHVWAAVKATQVRLYGR"
     gene            complement(1075..1881)
                     /locus_tag="PKJ61_11215"
     CDS             complement(1075..1881)
                     /locus_tag="PKJ61_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007078393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOC14430.1"
                     /translation="MIRPALPRWLAVPATAAAVFLAVPLLAMLVRVPWGRVPALLSTP
                     AAVDALWLSLRTCTLTVVVSLVLGLPLALVLSRTRGRWAAWGRVLATVPMVLPPVVAG
                     LALLVALSRRSWLGGWLASIGIDLAFTTAAVVIAQTFVAMPYLVVSVEGALRSAHDEH
                     AVIAATLGASPSVVLRRVTLPLIAPAVASGAALAFARALGEFGATLTFAGSLQGTTRT
                     LPLEIYLQRELDTDTAIALSVVLIAVAALLVAITARLSRGAVTSADGAAA"
     gene            complement(1878..2627)
                     /gene="modA"
                     /locus_tag="PKJ61_11220"
     CDS             complement(1878..2627)
                     /gene="modA"
                     /locus_tag="PKJ61_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017934502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HOC14431.1"
                     /translation="MKTCNLCAGLAAALLLTSCASTQPAAGGSELVVFAAASLNGTFT
                     ELGKEFEASHAGTTVKFSFDGSSALVDQLKQGAPADVFASADRSNMDKAVAAGVITGA
                     PEQFTSNVLTIIVPTGNPAAITGLDDSLTGKKLVVCANGVPCGSATVKLAEALGVTLS
                     PVSEETKVTDVRTKVESGQADAGIVYVTDAKASADLVESIPIQGADKARNDYLIGVAK
                     DAKQAALAAEFIALVKGSRGQEVLKAAGFGE"
     gene            complement(2624..3028)
                     /locus_tag="PKJ61_11225"
     CDS             complement(2624..3028)
                     /locus_tag="PKJ61_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007078395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HOC14432.1"
                     /translation="MTRLRISEAAALTGVSDDTVRRWSEQGKLSVSLDAANRQVVPGT
                     ELAALAAAHADSALDRLDEGAGRRSARNHFTGLVTRVISDQVMSQVELQCGPFRVVSL
                     ISTEAVQELGLEPGSVATAVVKATNVTVEAKR"
     gene            3179..5548
                     /locus_tag="PKJ61_11230"
     CDS             3179..5548
                     /locus_tag="PKJ61_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020576680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOC14433.1"
                     /translation="MTKTQQAENPADIHDLIRVHGARENNLRGIDIELPKRRLTVFTG
                     VSGSGKSSLVFDTIAAESQRLINETYSAFVQGFMPSLARPDVDLLDGLTAAIIVDQKR
                     MGGDPRSTVGTATDANAMLRILFSRLAEPHIGPPSAFAFNLATVHGAGAFTVERGAAT
                     KAEKATFTRTGGMCPRCEGRGTLSDFDLTALFDESLSLRDGALLVPGYSMDGWYGRIF
                     AGSGYFDMDKPLGGYTKKELHHLLYAEPKRIKVEGINVTYEGLIPKIRKSMLAKDPES
                     MQPHVRAFVERAITFGTCPDCDGTRLSEAARSAKLAGISIADACRMQISDLAEWVRGV
                     DAPNLGPLVGSLQQLLDFFTEIGLGYLSLERPSGTLSGGEAQRVKMIRHLGSSLTDVT
                     YVFDEPTAGLHPHDVQRMNNLLLRLRDKGNTVLVVEHEPETIAIADHVVDLGPGAGSA
                     GGTVCFEGSVAGLRASGTLTGRHLADQASLKAEVRTPTGSIEVRGASTHNLQSVDVDI
                     PLGVLVVVTGVAGSGKSSLVHGSIPAGAGVVSIDQAPIKGSRRSNPATYTGLLDPIRK
                     AFAKATGTKPALFSANSEGACPTCNGAGVVFTDLGFMASIASTCEDCEGKGFQASVLA
                     YRLGGRNIAEVLAMSVTAALEFFSDGEARTPAAVAVLARLADVGLGYLTIGQSLTTLS
                     GGEMQRLKLATRMGEKGGVYVLDEPTNGLHLADVEQLLELLDRLVDSGKSVIVVEHNQ
                     AVMAHADWLIDLGPGAGHDGGRIVFEGTPRDLVASGSTITAQHLAQYVR"
     gene            complement(5687..6496)
                     /locus_tag="PKJ61_11235"
     CDS             complement(5687..6496)
                     /locus_tag="PKJ61_11235"
                     /inference="COORDINATES: protein motif:HMM:NF025615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwiC-like family protein"
                     /protein_id="HOC14434.1"
                     /translation="MPASAQQRRSPGWKPKQHGAWFMLALPLVVGLILRPADAGSGWH
                     LAPLAGCWIIGYLAFTAATVWLKAPASRRAQHLRPVLVYGSVSGGFGIVTLALVDRGI
                     LWWLLAFLPLIGAALWLALLRRERTLASGALTVAAASLVTLVVCFDTPAALLAAWGTP
                     AANHALAMAALIFGYEFGTVFSVKTMIRERGDAGWLAASIGWHAFCTLGAAALAWSGA
                     AGWIWVVFFAATVVRAWLLPALGRTRTIRPLVIGLVEIGFTTAFVLAALLG"
BASE COUNT         1088 a         2326 c         2324 g          981 t
ORIGIN      
        1 cgcgtgcgct cgctcctcag cgaccgtaga ggcgcacctg ggtggctttg acggctgccc
       61 agacgtgggc gccggggcgt agaccgagtt cggctacgga catcgcggtc aagtcggcca
      121 ggagttcgtg gccggctacc gccaggtgga cgcggaccac ctggccacgg ggctcgatgc
      181 cggtcacctt ggcggccagg gtgttccgcg ggctgccacc aggaacggcc aggtggaggc
      241 tgatcgcttc gggctggatg ctggccaggc ccgccgcgcc gacggccaag ggctgctccg
      301 ggtcggcgag gccgcgcagc attactccgg gtgccacttc cagcagatcg gcaccgaccg
      361 cgaccccggc caccaggttc gtcccaccca gccgggcgag gaacgcgcta gcgggccgcg
      421 tgagcaactc ggcgaccggt ccgctctcca ccaccttgcc agcggtgatt acggccaact
      481 ggtcggcgat cgtccacagg tcgagcactt catgggtgac cagcagcgcc gtccgtccgc
      541 gcagccgggc ggcgagggtg tggcgtacct cggcggctac ggctacgtcc agtccggcca
      601 gcggctcgtc cagcagcacc acctctggct cggtcgccag cgcccgggcg atcgccaccc
      661 gctgggcctg cccgcccgag agcgcgtccg gacgccggga cgcgaactcc gcgcacccca
      721 ccgcctccaa ttcggtgagc gctcgcgtcc gcgccgcggc cggagaattg cctgcggccc
      781 ggggcccgaa ggccacgttc tccagcacgc tgaggtgcgg gaacagcgtc gcgcgttggg
      841 tcagcagtgc cacgcggcga cggtgcgggg gcaggcagcg atcccgcgaa gcgacctcgc
      901 ggccaccgat tcgcacttcg cctgatgagg gctgggcgag tccggctacg aggtgcagca
      961 ggctgctctt gccggcaccg ttcggcccaa ccagcgctac gaccgagccc tcggctacct
     1021 ccaggtcgag cgccacgccc cgggacctga tcaccgcatg gacggccagg ctgctcatgc
     1081 ggccgccccg tccgcgctgg tgaccgctcc tcgggacagc cgggccgtga tggccaccag
     1141 caatgcggca acggcgatca gcacgacgga gagcgcgatc gcggtgtcgg tgtcgagctc
     1201 gcgttgcagg tagatctcca gcggcagcgt ccgggtggtg ccttgcagtg atcccgcgaa
     1261 cgttagggtc gcgccgaact cgcccaatgc gcgggcgaac gccaatgcgg ctccggacgc
     1321 gaccgccggc gcgatcagcg gcaacgtcac ccgccgcaac accaccgacg gcgacgcgcc
     1381 cagggtggcg gctatgactg cgtgctcatc gtgcgcagag cgcagcgcgc cctctaccga
     1441 caccaccagg taaggcatgg ccacgaaggt ctgcgcgatc accaccgccg cggtggtgaa
     1501 ggccaggtcg atgccgatcg aggccaacca gccgcccaac caggatcgcc ggctcagcgc
     1561 caccagcagg gcaaggccgg caacgactgg cggcagcacc atcggcaccg tggccagcac
     1621 gcgtccccag gccgcccagc gtccgcgcgt ccgggagagc accaacgcca gtggcaggcc
     1681 cagcacgagt gagaccacca cggtcagcgt gcaggtgcgc aggctcagcc acagcgcgtc
     1741 caccgcggcc ggggtgctca acagcgccgg gacgcgtccc cacggcaccc gaaccagcat
     1801 cgccagcagg ggtaccgcga gaaagactgc ggctgcggtg gcgggcacgg ccagccaccg
     1861 cggcagcgcg gggcggatca ctccccgaag ccggcggcct tcagcacctc ctggccgcgg
     1921 ctgcccttca ccagcgcgat gaactcagcg gccagcgcgg cctgcttggc gtccttggcc
     1981 acgccgatca gatagtcgtt gcgggccttg tccgccccct ggatcgggat gctctccacc
     2041 aagtcggcgg acgccttcgc gtcggtcacg tagacgatgc cggcgtccgc ctggccggat
     2101 tcgaccttcg tccgcacgtc ggtgaccttg gtctcctcgg agaccgggct cagggtcacg
     2161 cccagcgctt cggcgagctt cacggtggcc gaaccgcacg gcacgccgtt ggcgcagacc
     2221 acgagcttct tgccggtcag ggagtcgtcc agtccggtga tcgccgcggg attgcccgtg
     2281 ggcacgatga tcgtcagcac attgctggtg aactgctccg gagcgccggt gatcacccct
     2341 gcggcgaccg ccttgtccat gttcgagcgg tcggcggacg cgaacacgtc cgccggtgcg
     2401 ccctgcttga gctgatcgac cagcgccgac gagccgtcga aggagaattt caccgtagta
     2461 ccggcgtggg acgcctcgaa ctctttgccg agttcggtga aggtgccgtt cagggacgcg
     2521 gccgcgaaca ccacgagctc ggagccgccg gctgccggct gagtcgaagc gcacgaggtg
     2581 agcagcaggg cagcggccag gccggcgcac aggttgcagg tcttcatcgc ttcgcctcca
     2641 cggtcacgtt cgtcgccttg accacggccg tggccacgct gcccggctcc aggccgagtt
     2701 cctgcactgc ttcggtggag atcagcgaca ccacccggaa cgggccgcac tgcagttcca
     2761 cctgcgacat cacctggtcg gagatgacgc gggtcaccag cccggtgaag tggtttcgcg
     2821 cgctccggcg tccggcgccc tcatcgagcc ggtccaaggc ggaatcggcg tgcgcggccg
     2881 ccagcgcggc aagttctgta ccgggtacga cctggcgatt ggcagcgtcc aggctcacgc
     2941 tgagcttgcc ctgctcgctc cacctgcgga ccgtgtcatc gctgaccccg gttagcgcag
     3001 cggcctcact gatccgtagt cgcgtcatga ccggaaccct atctccgcga atgcgtagtt
     3061 gaaagctgtc tggagcgcga ggtcgggtgc gaaggcagca atccctcgcc ggcgcggggt
     3121 gttgtcggca tcggcgatct cgtgagagat tggccaactc gccgcggacg gagacctcat
     3181 gacgaaaacc cagcaggcgg agaatcccgc cgacatccac gacctgatcc gcgtccatgg
     3241 cgcgcgggag aacaacctgc gcggcatcga tatcgaactg ccgaagcggc ggctgaccgt
     3301 gttcaccggc gtatccggct cgggcaagag ctcgctggtg ttcgacacca tcgcggccga
     3361 atcgcagcga ctgatcaacg agacctacag cgccttcgtg cagggcttca tgcccagcct
     3421 cgcccgtccg gacgtggacc tgctggacgg cctgaccgcc gcgatcatcg tcgaccagaa
     3481 gcggatgggt ggcgacccgc gttcgaccgt gggcaccgcc accgacgcca atgcaatgct
     3541 gcgcatcctg ttcagccgcc tggcggagcc gcacatcggt ccgccgagtg cgttcgcgtt
     3601 caacctggcc accgtccacg gtgctggagc gttcaccgtc gagcgcgggg cggccaccaa
     3661 ggccgagaag gccaccttca cccgcaccgg cggcatgtgc ccacgatgcg agggccgcgg
     3721 cacgctctcc gacttcgatc tgaccgcgct cttcgacgag tcgctctcgc tgcgcgacgg
     3781 cgcgttgctg gtgcccggct acagcatgga cggctggtac ggccgcatct tcgccggcag
     3841 cggctatttc gacatggaca agccgctggg cggctacacc aagaaggaat tgcaccatct
     3901 gctctacgcg gagcccaaac ggatcaaggt agagggcatc aacgtcacct acgaggggct
     3961 gatcccgaag atccggaagt cgatgctggc caaggatccg gagtcgatgc agccccacgt
     4021 ccgcgccttc gtcgagcggg cgatcacctt cggcacctgt ccggactgcg acggcacccg
     4081 gctgagcgag gctgcacgct cggccaagct ggccggaatc agcatcgcgg acgcctgccg
     4141 gatgcagatc agcgacctcg ccgaatgggt gcgcggcgtg gacgcgccga atctgggccc
     4201 gctggtcggt tcgctgcagc agttgctgga cttcttcacc gagatcgggc tcggctacct
     4261 ctcgctggaa cggccgtcgg gcacgctttc tggcggcgag gcgcagcggg tgaagatgat
     4321 ccgccacctc ggatcatctc tgaccgatgt cacctatgtc ttcgacgagc cgaccgccgg
     4381 cctgcatccg cacgacgtgc agcgcatgaa caacctgctg ctgcggctgc gggacaaggg
     4441 aaacaccgtc ctggtggtcg agcacgaacc ggagacgatc gcgatcgccg accacgtcgt
     4501 cgacctcggt ccgggcgccg gcagtgccgg tgggacggtc tgctttgagg gctcggtcgc
     4561 cggactgcgg gccagtggga cgctgaccgg acggcacctg gccgatcagg cctcgctgaa
     4621 ggccgaagtg cggacgccga ccgggtctat cgaggtgcgc ggcgcgtcca cccacaacct
     4681 gcagagcgtt gacgtggaca ttccgctcgg ggtgctggtc gtggtcacgg gcgtcgcggg
     4741 gtcggggaag agttcgctgg tgcacggctc cattccggcc ggcgccggcg tggtgtcgat
     4801 cgaccaggcg ccgatcaagg gctcgcggcg cagtaatccg gccacctaca ccgggctgct
     4861 cgatccgatc cggaaggcgt tcgcgaaggc caccggcacc aagccggcgc tgttcagcgc
     4921 gaactccgaa ggggcttgtc cgacgtgtaa tggcgctggt gtggtgttca ctgatctggg
     4981 tttcatggct tcgatcgcgt ccacgtgcga ggactgtgaa ggcaagggct tccaggcgtc
     5041 cgtactggcc taccggctgg gcggacggaa catcgccgag gtgctggcga tgtcggtcac
     5101 cgcggcgctg gagttcttct cggatggcga ggcgcggacg ccggccgcgg tcgcggtgct
     5161 ggcacggctg gccgacgtcg gtctcggcta cctgaccatc gggcagtcgc tgaccacctt
     5221 gtccggcggc gagatgcagc ggctgaagct ggccacccgg atgggggaga agggcggggt
     5281 gtacgtgctc gacgagccca cgaacggcct gcacctcgcc gatgtggagc agttgctgga
     5341 gttgctcgac cggctggtgg actccggcaa gtcggtgatc gtggtcgagc acaaccaggc
     5401 ggtcatggcg cacgccgact ggctcatcga cctcggcccg ggtgccggcc atgacggcgg
     5461 ccggatcgtg ttcgaaggca ccccgcggga cctggttgcc tccggctcca cgatcaccgc
     5521 ccagcatctg gcccagtacg tccgctaggt agacgctgat caacgatccc tgagcttgac
     5581 gaagggtcgt ccatccgtac ggtgactacg ggcttcgtca ggctcagccg tcgttgaacg
     5641 cggtgactac gggcttcgtc aagctcagcc agcgttggcg ctggggttag ccgagcaggg
     5701 cggccagcac gaaggcggtg gtgaacccga tctcgaccaa cccgatgacc agcggacgga
     5761 tcgtccgggt gcggccgagg gctggcagta accaggcgcg cacgacggtg gcggcgaaaa
     5821 agacgaccca gatccagccg gccgcgcccg accaggccag cgcggctgcg cccagggtgc
     5881 agaacgcatg ccagccgatc gaagctgcca gccagccggc gtccccgcgc tcgcggatca
     5941 tggtcttcac tgagaacacg gtgccgaatt cgtagccgaa gatgagcgcg gccatcgcca
     6001 gcgcatggtt cgcggccggc gttccccacg cggccaacag cgctgccggg gtgtcgaagc
     6061 agaccaccag ggtcaccagg ctcgcggcgg ccacggtcag cgcgccgctg gccagcgtcc
     6121 gctcccgtcg cagcagggcc agccacagcg ccgccccgat cagggggagg aacgccagca
     6181 gccaccagag aatgccgcgg tccaccaggg cgagcgtcac gatgccgaac cctccggaca
     6241 cgctgccgta caccagcaca ggacgcaggt gctgagccct ccggctcgcg ggggccttca
     6301 accaaacggt ggcggcggtg aaggcgaggt aaccgatgat ccagcagccg gccagcgggg
     6361 ccaggtgcca gccgctacct gcgtccgctg ggcgcaggat caggcccacc accaggggta
     6421 acgcaagcat gaaccaggcg ccgtgctgct tcggtttcca ccccggcgaa cggcgttgct
     6481 gggcggaggc gggcatggca gcgatcgtag cctgacgcga cgcgacccgg ccgacccccg
     6541 tcgcgggagt cggccatcgt tcactgagtt gcgcgcgaag ctgccgcagt acctgcgtta
     6601 gtcatggggc agcggaccct ccgaagcggt cgtgtgcatc ccgacggctt ctgcagccgt
     6661 tctggagcgg atgacgggaa tcgaacccgc atggccagct tggaaggctg gggctctgc
//
LOCUS       DAOPRQ010000107         6614 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_9595_length_6614_cov_10.576002, whole genome shotgun sequence.
ACCESSION   DAOPRQ010000107 DAOPRQ010000000
VERSION     DAOPRQ010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 6614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6614
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9595_length_6614_cov_10.576002"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(268..690)
                     /locus_tag="PKJ61_11240"
     CDS             complement(268..690)
                     /locus_tag="PKJ61_11240"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOC14435.1"
                     /translation="MKYRKLGHTGLEISPICVGCMSFGVPGRGSHAWTLGEDDSREVI
                     RGAVEAGINFFDSANVYSDGTGEEIRGRALRDFGRRDEPVIATKVYYPTGEGPNRSGL
                     SRKAIFTEVDNSRRRLGPTTSTSTRPISGTRPHHLKRP"
     gene            830..1690
                     /locus_tag="PKJ61_11245"
     CDS             830..1690
                     /locus_tag="PKJ61_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004808989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOC14436.1"
                     /translation="MKIAGAPISWGVCEVPNWGYQMAPERVMAEMREIGLTATEFGPQ
                     GWLPVDAEARAVEVAKFGLTPVGAFFLAVMHDPAIDPIPAVLAELRAFEVAGGSYLIL
                     AAASGAEGYDARPVLDDAGWQTLFENLDRISAVCAAKGVTACLHPHWGTMVQNADEVD
                     RVLRNSSVGLCLDTGHITCGGGDPAAIVAEFADRVEIVHAKDVRKSLTDQLLTSGLTW
                     SEGIRAGMFTPIGEGDIDFAAIVGRLAEAGFDGYYVLEQDIMIDDEPPAGGGPVANAR
                     KSLAALRALA"
     gene            complement(1764..2951)
                     /locus_tag="PKJ61_11250"
     CDS             complement(1764..2951)
                     /locus_tag="PKJ61_11250"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOC14437.1"
                     /translation="MDASQVFVRTSSQRALRENPWSVDCRRRRPARAVGGKELDVMSG
                     DAEADQGAGEARRMHRPTMGDVAEAAGVSRPLVSIVMRDAKGASEATRQRVLKVAAEL
                     GYVPDDRARKLRQKSSRLIGVAFWLQHPFHGDVVEQLYLAAAEQGYDLALSAVAPTRD
                     ESTAIESLIRERCEALIVLSLKEEAKHLQTVSKRVPVISVARRLKESTVGVVRGDDRA
                     GLRMAVEHLASLGHTRIAHIDGARVPGSVERRSSYRKTMQRLRFDEHIDVVLGGPTEE
                     DGARGMAELLSRSVRPTAVIGFNDRCAAGALDYAIRAGLSVPQDISIMGYDDSRLSRR
                     THVQMSTISQDTQTQAHQAVRLAIEQVNGMPAREVIVQPQLVARETTAAPPDSAAEIS
                     RRS"
     gene            3053..4063
                     /locus_tag="PKJ61_11255"
     CDS             3053..4063
                     /locus_tag="PKJ61_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOC14438.1"
                     /translation="MRVALIGAGRIGSSHAELLANRVPGVELAAIYDPIEGAAARLAA
                     KLAVPFATHNIDDIWSDPAIHAVAIAAPARSHTDLVVAAAGAGKHVFVEKPMAVTLED
                     ADRAIAAAEASGVILQVGFNRRYAAGFAAARAAVDAGVVGEPQLLRSLTRDPGPFMGD
                     PTRIPQWTIFFETLIHDFDTVCWLNPNAKPVRVTALADALVRPDGKEIGHLDTSVVTI
                     EFDNGAIAVAEANFCAMYGYDVRGEVFGSAGMCTAGDVRATNLTAYTATGISVETSRL
                     DSDLLRDAYLGEFEAFATAIRAGGPSPVPGSAARLALAIALASIKSVQEKRAVNIEEV
                     DR"
     gene            4060..4833
                     /locus_tag="PKJ61_11260"
     CDS             4060..4833
                     /locus_tag="PKJ61_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOC14439.1"
                     /translation="MSYRLAASSEMIYLDRPFLERVERIRDRGLLVEIWDWSNKDIDA
                     LANSGAEIVSMTGYLTGTLTDDDGIEQFLATAEQSIEVAKRLNCPSLNVHGTGLGEGG
                     LPVKPVQVVTPGMWLRAADTLRRLAELGAKHDKVFTLENLNLPVDHPGTPFALARDTK
                     TLVAAVDSPHLRMNLDLYHAQIGEGNLIELVRECQPWIGEIQVADVPGRCEPGTGEVN
                     YAAVAAALKDIGYSGVVAMEAFAKGDSDVALDAFCAAFS"
     gene            4876..5631
                     /locus_tag="PKJ61_11265"
     CDS             4876..5631
                     /locus_tag="PKJ61_11265"
                     /inference="COORDINATES: protein motif:HMM:NF019322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOC14440.1"
                     /translation="MIIETQNPAFTVLLDRASPSPLYHQLVQQFTAAIESGVLKPGEA
                     LEREDLFAARLAVARPTLRRALVELASKGLITRSRGRGTEVVGPFSRTVQPAREAWLA
                     CPEPSDQAVLRLVRLEPDRVEPRAALDLGLDRDGALVYLEQLEVDQDKRISFRKEWLP
                     GSLVGPVLHNLSTTPVRRVLADIGHATVRDQRTFEFRPPDATEKAALGLDDSERIFAV
                     RIVAFDQGGTPIEVATVVYVNEPALDHDLRRAS"
     gene            5877..>6614
                     /locus_tag="PKJ61_11270"
     CDS             5877..>6614
                     /locus_tag="PKJ61_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOC14441.1"
                     /translation="MTVVSTDAAAVPSASAGEVLLEARGVGKLFPGVRALNNVDFVLR
                     GGEVHALVGENGAGKSTLMKIFAGLYQPDGGEVRLRGDRIVLPTPLSAHHAGISVIHQ
                     EFFLMNHLTVAQNIFIGREPKLPGGVLNDDRMLNRQAAELLDRLGVDIDPKLQVGRLT
                     VAQQQMVEIAKALSFNASVLIMDEPTAALTETEVATLFRIIDNFVTPTTAVVYISHRM
                     EEIKKISTQVTILRDGELVGTHPAAELS"
BASE COUNT         1127 a         2125 c         2196 g         1166 t
ORIGIN      
        1 gttgacagcg gccaactcgt ccgcggtcaa gacgagattt agggctgcga gcgcgtccgt
       61 caggtgctgg ggcttggtca cggtcggcgg actcgtcgta gagcgcaccc tgcatctcgt
      121 cggtctcgga gcgggcggtt tcagcagtcc agtcccgggt gagcttgccg cgagccagcg
      181 ggctccacgg catcgcggcg actcccaacg cggccgtcgc ggggtagctt tcgcgctcct
      241 cctcgcggtt gagcaggttg tagtaactca gggtctcttc aagtggtgtg gccgcgtccc
      301 agaaatgggt cgggtagagg tcgatgtagt cggtccgaga cggcgccggg agttgtcgac
      361 ctcggtgaag atcgccttgc gggacagccc cgagcggttg gggccctcgc cggtcgggta
      421 gtagaccttg gtcgcgatca ccggttcgtc ccggcggccg aagtcgcgca gggcgcgtcc
      481 gcggatctcc tcaccggtac cgtcggagta gacgttcgcg gagtcgaaga agttgatccc
      541 ggcttccacc gcgccgcgga tcacttcgcg cgagtcgtcc tcgccaagcg tccaggcatg
      601 cgaaccacgg cccgggacgc cgaagctcat gcatcccacg cagatgggtg agatctccaa
      661 gccagtgtgg ccaagcttcc gatacttcat gaccgcgcct tagggcgccg gtgggtgctg
      721 agacaggtac tgggagtagc ctgttggagg cgaacccgct ctagttgtac tggtcgtcgc
      781 gcgcaagtag cacgtgctag cacagccccc aagaaatagg agcaaatcaa tgaagatcgc
      841 tggagccccg atcagctggg gcgtgtgtga ggtaccgaac tggggctatc agatggcacc
      901 ggagcgagtc atggccgaaa tgcgcgagat cggcctaact gcaaccgagt tcggcccgca
      961 aggttggctg ccggttgacg ctgaggcacg ggccgttgag gtagcgaagt tcgggctgac
     1021 gccggtgggt gcgttcttcc tcgccgtgat gcatgatccg gcgatcgatc cgatccctgc
     1081 ggtgttggcc gagttgcgcg cattcgaggt ggccggcggc tcctacctga tcctcgccgc
     1141 ggcgtccgga gcggagggct acgacgcccg tccggtgctg gacgatgccg gctggcagac
     1201 cctcttcgag aacctcgatc ggatcagcgc cgtctgcgcg gccaagggtg tgaccgcgtg
     1261 cctgcacccg cactggggca ccatggtgca gaacgccgac gaggtcgacc gcgtgctgcg
     1321 caacagttca gttgggcttt gtctcgacac cggccacatc acctgcggtg gcggtgaccc
     1381 ggcggcgatc gtggccgagt tcgccgaccg ggtcgagatc gtccacgcca aggacgtgcg
     1441 caagtcgctt accgatcagc ttctgaccag tggactcacc tggtcagagg gcatcagggc
     1501 cggcatgttc accccgatcg gtgaaggtga catcgacttc gcggcgatcg tcgggagatt
     1561 ggccgaagcg ggcttcgatg gctactacgt gctggagcag gacatcatga tcgacgacga
     1621 gcctccggca ggcggcggcc cggttgccaa cgcccgcaag tcgctggctg ctctgcgcgc
     1681 cctcgcctga gcacgggcgt ctcccggctg gcaacgtcat aacggctggc caacgtcatc
     1741 ccggcgaagg ccgggatctc tcgtcacgac cgacgcgaga tttcggccgc gctgtccggc
     1801 ggtgctgccg tcgtttcccg tgcaaccagc tgcggctgca cgatcacctc gcgtgccggc
     1861 atcccgttga cctgttcgat cgccagccgc acggcctggt gggcctgggt ctgggtgtcc
     1921 tgcgagatcg tgctcatctg gacgtgagtt ctccgcgata gccgggagtc gtcatagccc
     1981 atgatcgaaa tgtcctgggg gacgctgagc ccggcgcgga tggcgtagtc gagcgccccg
     2041 gcggcacaac ggtcgttaaa accgatcact gcggtgggtc gtacagatcg gctgaggagt
     2101 tccgccatgc ctcgagcgcc atcttcttcg gtggggccgc ccaacaccac gtcgatgtgc
     2161 tcgtcgaacc gaaggcgctg catggtcttt cggtagctcg acctacgttc gacactgcca
     2221 ggcacccgcg cgccgtcgat gtgtgcgatc cgggtatggc caaggctggc gagatgctcc
     2281 acggccatgc gcaggcccgc acgatcatcg cccctcacca ccccgacggt ggactccttc
     2341 aggcgcctcg caaccgagat caccggaacc cgcttcgaca ccgtctgtag gtgcttggct
     2401 tcttccttga gactcagcac gatcagggcc tcgcagcgct cgcgaatcag cgactcgatc
     2461 gccgtgctct cgtcccgggt gggggcgact gcgctcaggg caaggtcgta gccctgctct
     2521 gcggcggcca ggtacagctg ctcgaccacg tcgccatgga aggggtgctg cagccagaag
     2581 gcaacgccga tcagccgcga cgacttctgc cgcagcttgc gagcgcggtc gtcgggcacg
     2641 tagcccaact ccgcggcgac cttcagaacg cgttggcggg tggcttcgct ggcccccttg
     2701 gcgtctcgca tgacgatgga gacgagggga cgggatacgc cggcggcttc cgcaacgtcc
     2761 cccatcgtgg gccggtgcat tcgacgcgcc tcgccggcgc cctggtccgc ttcggcgtcc
     2821 ccgctcatga cgtccagttc cttcccgccg acagcccgcg ccgggcgccg cctgcggcag
     2881 tctaccgacc agggattttc gcgcagggcc cgttggctag atgttctgac aaacacttga
     2941 ctcgcgtcca tctttggctc acactagcac gtgttactag cacgtgtgag attggagaac
     3001 agagaacatg tcagttgacg acgaagtcac tggtccaggc aatccttggc cgttgcgcgt
     3061 tgccctgatc ggtgccgggc ggatcggcag cagccacgca gagctgctcg cgaaccgagt
     3121 gcccggcgtc gagttggccg ctatctacga cccgatcgaa ggggcggcag ccagacttgc
     3181 cgccaagcta gccgtgccct tcgccaccca caatatcgac gacatctgga gcgatcccgc
     3241 tatccatgct gtcgccatcg ccgctcccgc acggtcccac actgatctgg tggtggccgc
     3301 cgcaggcgcc ggcaagcacg tgttcgtgga gaagccaatg gcggtaaccc tcgaagacgc
     3361 cgaccgggcc atcgctgccg ccgaagcgtc cggcgtgatc ctgcaggtcg ggttcaaccg
     3421 gcgctacgcc gcaggcttcg ctgccgcccg cgccgccgtg gacgccggcg tggttggaga
     3481 gccgcaacta ttgcggtcgc tcacccgcga cccagggccg ttcatgggcg accccacccg
     3541 gatcccccaa tggacaatct tcttcgagac cctgatccat gacttcgaca cggtgtgctg
     3601 gctcaacccg aacgcgaagc cggtgcgggt caccgccctg gccgacgcgc tcgtccgtcc
     3661 ggatggcaag gaaatcggcc acctcgacac gtcggtcgtg accatcgagt tcgacaacgg
     3721 tgcgatcgcc gttgcggagg ccaacttctg cgcgatgtat ggctatgacg tccgcggcga
     3781 ggtcttcggc tcggccggta tgtgcacggc cggcgacgtc cgagccacca acctgaccgc
     3841 gtacacagcc acgggaatca gcgtcgagac ctcccgcctg gattccgacc tgctgcggga
     3901 cgcgtacctg ggtgagttcg aggcattcgc cactgccatc cgggctggcg gaccgtcccc
     3961 ggttcccggt tccgcggcgc gcctcgcctt ggcgatcgcg ctcgcgtcca tcaagagcgt
     4021 gcaggagaag cgcgcggtga acatcgagga ggtcgaccga tgagctaccg tcttgccgcc
     4081 agttcggaga tgatctacct cgaccggcca tttctggagc gggtcgagcg cattcgtgat
     4141 cggggtctcc tggtagagat ctgggactgg tcgaacaagg acatcgacgc cctcgccaac
     4201 agcggcgcag agatcgtctc catgaccggc tacctcaccg gcaccctcac cgacgacgac
     4261 ggcatcgagc agttcctcgc cacggccgag cagtcgatcg aggtggccaa gcggctgaac
     4321 tgcccgagcc tgaacgtgca cggtaccggc ctgggtgagg gcgggctgcc ggtgaagccg
     4381 gtccaggtgg tcacgccggg catgtggttg cgcgccgccg acaccctgcg ccggctcgct
     4441 gaactgggag ccaagcacga caaggtgttc accctggaga acctcaacct tccggtcgat
     4501 caccccggta cccccttcgc gctggcgcgc gacaccaaaa ccctcgtcgc tgcggtcgac
     4561 tccccgcacc ttcgcatgaa tctcgacctg taccacgccc agattggtga ggggaacctg
     4621 atcgagttgg tgcgggagtg ccagccgtgg atcggcgaga tccaggtcgc cgacgtgcct
     4681 ggacgttgtg agccggggac gggtgaggtg aattacgccg cggtcgccgc tgcgctcaag
     4741 gacatcggct actccggcgt ggtggccatg gaggccttcg ccaagggtga ttccgatgtc
     4801 gctctggacg ccttctgcgc ggccttcagc tgacgccgac acgaaaccca cagcggcgac
     4861 ggttaggaga agaaggtgat catcgaaacg cagaaccctg cgttcactgt gctcctcgat
     4921 cgcgcgagcc cgtcgccgct gtaccaccaa ctcgtgcagc agttcacggc cgcgatcgag
     4981 agcggtgtgc tgaaacccgg cgaagcgctg gagcgggaag acctcttcgc ggccagactc
     5041 gccgtcgccc ggccgaccct ccggcgggcc ttggtcgagt tggcaagtaa ggggctgatc
     5101 accaggtcgc gcggccgggg caccgaagtg gtcggtccct tttctcggac ggttcagccc
     5161 gcccgagagg catggctggc ttgccccgaa cccagtgatc aggctgtcct acggcttgtt
     5221 cgactcgagc cggaccgagt cgagccgcgg gctgcgctcg acctcggcct ggatcgggac
     5281 ggtgcgctgg tctacctcga gcagttggaa gtggaccagg acaagcggat cagcttccgt
     5341 aaggagtggt tgcctggctc gctggtgggt ccggtgctgc acaacctgtc cacgacgccg
     5401 gtgcgacggg tccttgccga cattgggcat gccacagtgc gggaccagcg cacctttgag
     5461 ttccggcctc cggacgcgac cgagaaggca gccctgggtc tggatgactc ggagcggatc
     5521 ttcgcggtgc ggatcgtcgc cttcgatcaa ggtggaactc cgattgaggt ggcaacggtg
     5581 gtctacgtga atgagccggc gctggaccac gatctgcggc gtgccagcta gttatcgccg
     5641 gacgtgaacc agcgggcatc cggcactgac tagcacgtga aagcaacagt tagataacac
     5701 ctgctagcag gttcgtaaca atgtattgac aaagcggttc agatgctaga acgtgctata
     5761 cgaagtcgtc gctgatggtc ggggcgagtt cgtcgggcac agacccgacc ctcgatcggc
     5821 aatccgagaa cgacggtcac tagagacatg cgacaccaac agcgaggagg ctgaagatga
     5881 cggtggtcag cacggacgcg gcagcagtac cgtccgccag tgcgggcgag gtgctgctgg
     5941 aagcgcgcgg ggtcggcaag ctatttcccg gcgtgcgggc cttgaacaac gttgacttcg
     6001 tcctgcgtgg tggcgaggtg cacgctctgg tgggcgagaa cggcgccggc aagtcgacgt
     6061 tgatgaagat cttcgccggt ctgtaccaac ccgacggggg cgaggtgcgg ctacgtggag
     6121 atcggattgt gctgcccacc ccattgtcgg cacaccatgc cgggatcagc gtgatccatc
     6181 aggagttctt cctgatgaac cacctcacgg ttgcccagaa catcttcatc ggtcgggagc
     6241 cgaagctgcc cggcggcgtc ctcaacgatg accgcatgct taaccggcag gctgcggaat
     6301 tgctcgaccg gctcggcgtc gatatcgatc caaagctgca ggtgggccgg ctgacagtgg
     6361 cgcagcagca gatggtcgag atcgccaagg cgctgtcatt caacgcttcg gtgttgatca
     6421 tggacgagcc cacggccgcg ctcaccgaga ccgaggtggc gaccctgttc cggatcatcg
     6481 acaacttcgt cacccccacg accgcggtgg tctacatctc gcaccggatg gaggagatca
     6541 agaagatctc cacccaggtg accattttgc gggacgggga gttggtcggt actcatccgg
     6601 cagctgagct cagc
//
LOCUS       DAOPRQ010000108         6520 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_9757_length_6520_cov_10.308121, whole genome shotgun sequence.
ACCESSION   DAOPRQ010000108 DAOPRQ010000000
VERSION     DAOPRQ010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 6520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6520
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9757_length_6520_cov_10.308121"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="PKJ61_11275"
     CDS             complement(<1..692)
                     /locus_tag="PKJ61_11275"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HOC14442.1"
                     /translation="MTQIWAHRGARAEAPENTLPAFELAIEQQADGVEFDVQFTADNE
                     LVVIHDETVARTTDGQGRVVAYSLAQLQRLDASAGKPEFAGVQIPTLAQVLEVLKPTN
                     LELNIELKNSVEDYPGLEEAVLAMVAEFGLADRVVLSSFNHYSLKKLQSLGAPSPIGM
                     LYSDPLYRPWRYAAKLGVDALHPPARYVTSARFVRKAHAAGLAVRPWIVNGERRLERM
                     FRWGVDAVFTDYP"
     gene            complement(750..1004)
                     /locus_tag="PKJ61_11280"
     CDS             complement(750..1004)
                     /locus_tag="PKJ61_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014847421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14443.1"
                     /translation="MPELHQSARKHFRRDRLDDATVRYAASHVVHCRPLDEEDVPRRW
                     LMIGTDTAGRLLELVVLVYDDGYELVIHAMKARPQYLDEI"
     gene            complement(997..1272)
                     /locus_tag="PKJ61_11285"
     CDS             complement(997..1272)
                     /locus_tag="PKJ61_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HOC14444.1"
                     /translation="MRMSEHLLHGQPVTDEQIQAWADEAEAGYDLAKLPKPRRGRPPV
                     GDGPGVVVPVRLDAATLAALTARAEAEGIATRSDAIRAAVREWSHVA"
     assembly_gap    1413..1512
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1513..2574
                     /locus_tag="PKJ61_11290"
     CDS             <1513..2574
                     /locus_tag="PKJ61_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HOC14445.1"
                     /translation="YGELVTFLTDELAAQAPADDAVGRDRYALWSREFVGAAVDLDET
                     YEWGLEELERMAAEQDRIVAEIAGPGASIEDAIAKLETDPARVLHGKDALREWMQSTA
                     DEAIAALDGVHFDLAEPIRKIECMIAPTENGGIYYTGPSDDFSRPGRMWWSVPPGVTE
                     FSTWREKTTVYHEGVPGHHLQIAQAVLNRSQLNSWRRLVSWSSGHGEGWALYAERLME
                     EFGFMDDLGDRLGLVDGQRMRATRVVLDIGVHCRKPAPERWGGGTWDAAKAWECFKAN
                     VNMDESFLRFELNRYLGWPGQAPSYKIGQRLWETYRAQASAAAAAAGEEFSLKDFHTR
                     ALNLGSVPLAVFPEALGLS"
     gene            complement(2797..3711)
                     /locus_tag="PKJ61_11295"
     CDS             complement(2797..3711)
                     /locus_tag="PKJ61_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HOC14446.1"
                     /translation="MTEPLHRSWRPAWPCPVGQVWGTFKRGLADPTYKVIDGRHWRAL
                     NTPVGTTTLAVRTLDASGVIEAEAWGDGAAWALDDLPQMLGASDDPSGFRPSHQLLAE
                     LHRSRPHWRIARTASVWDALVPVVIEQKVTGAEATYGIRALLVRYGVPAPGPGEQLGL
                     RTFPTADALRRIPSWDWLKMHIDPARSRTLLTAARVADTLNRVLPAEPERADERLRSL
                     PGIGIWTSAEVRARALGDADALSLGDYNVPRDIGWALVGQPLDDAGLAELLEPYRPHR
                     LRVQRLIGMAGIAPPRHGPRLAPRTHLP"
     assembly_gap    3713..3812
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3825..5330
                     /locus_tag="PKJ61_11300"
     CDS             3825..5330
                     /locus_tag="PKJ61_11300"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017933718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0071897 - DNA biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HOC14447.1"
                     /translation="MLLDQVVQTSARVAATSSRTAKVAALAESLAAASADELEIVAHY
                     LSGSLRQRRTGVGWRSLLSRPEAADAPTLTLTEVDAAFGELAGLGGAGSQGARQASLA
                     QLFGRATPAEQAFLSGLITGETRQGALVGLLLPAIASAFAVPVEAVRRAAMFAGSIPV
                     VAMTAQAGGEAGLAEITLQVGRPVLPMLASSAPSIEAALGRFGAGTPVAVEAKLDGIR
                     IQVHREGDAVGVYSRSLDDITARLPAVVAAVARLPASRLVLDGEALALRPDGRPMLFQ
                     DTASAQAELVPFFFDLLHVDEVDLVDQPMSVRQAHLAALLPAELQVARLVTTEPGDAE
                     DFSRQVLAAGHEGVVIKALDAGYQAGRRGAAWVKVKPVHTLDLVVLAVEWGSGRRRGL
                     LSNIHLGARDPGSGGFVMLGKTFKGMTDEMLAWQTERFLALETHREGHVVFVRPEQVV
                     EVAFDGVQRSTRYPGGMALRFARVVRYRDDKAADDADTVDVIRDLATREPR"
     regulatory      5456..5569
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00634"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class IV; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00634"
     gene            5582..5911
                     /locus_tag="PKJ61_11305"
     CDS             5582..5911
                     /locus_tag="PKJ61_11305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14448.1"
                     /translation="MTDSCDRFGPGHNVHWIQRGLASRHLYDEPPLRATFMWRDGCEV
                     DVWLEDHLERWRFHDGRQITRLVEAWKLDTVAYLLPHGLLTLVGVGCVYPCRDESQWR
                     ACTSRQT"
     gene            complement(5992..>6520)
                     /locus_tag="PKJ61_11310"
     CDS             complement(5992..>6520)
                     /locus_tag="PKJ61_11310"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOC14449.1"
                     /translation="TIHPFVDGNGRTGRALLHAHLRNKQLVRRVTIPVSAGLLADTNA
                     YFKALTAYRQGDIEPIVSQLAAASIRAAGLGRWLVDQLADVRDGWLKRLPSRTGSAGR
                     RLASILVGQPAVNVTFVVERLGVSATAARRAIDQATAAGLLVETSDRKRNRVWIAREA
                     LAVFDEFSDRAGRRV"
BASE COUNT         1016 a         2033 c         2236 g         1035 t
ORIGIN      
        1 ggatagtcgg tgaagaccgc gtccacaccc cagcggaaca tgcgctccag gcgccgctca
       61 ccgttcacga tccacggacg caccgccagg ccggcagcgt gcgccttgcg gacgaaccgg
      121 gcgctcgtga catacctggc cgggggatgc agcgcgtcca cgcccagctt cgcggcgtag
      181 cgccacggcc ggtacagcgg atcggaatac agcatcccga tcggcgaagg cgcgccgagt
      241 gactgcaact tcttcagcga gtagtggttg aagctcgaca gcaccacccg atcggccagg
      301 ccgaactcgg ccaccatcgc cagcacggcc tcctccaagc ccggatagtc ctccaccgag
      361 ttcttcagct cgatgttgag ctcaaggttg gtcggcttga ggacctccag cacctgggcg
      421 agcgtcggaa tctgcacgcc ggcgaactca ggcttcccag cggacgcgtc cagccgctgc
      481 aactgcgcca acgaataggc caccacccgg ccctggccgt cggtcgtccg cgccaccgtc
      541 tcgtcgtgga tcaccaccaa ctcgttgtcc gcggtgaact ggacgtcgaa ctcgaccccg
      601 tccgcctgct gctcgatggc caactcgaac gccggcaacg tgttctccgg cgcctctgcg
      661 cgggcgccgc ggtgggccca gatctgggtc atgtcactcc tggcattcaa cgttcatcac
      721 tccgtggtct cgccacccat catggccgat cagatctcgt ccaggtattg cggacgagcc
      781 ttcatagcgt ggatgaccag ctcgtatccg tcgtcataga ccaagacaac aagttcaagc
      841 agacgccctg ccgtgtccgt accgatcatg agccatcgac gcggaacgtc ctcctcgtcg
      901 agcgggcggc agtgcaccac gtgcgaagcg gcgtagcgga cggtggcgtc atcgaggcga
      961 tcacgccgga agtgcttccg agcggactgg tgcagttcag gcaacgtgcg accactcgcg
     1021 cacagcagcc cggatcgcat ccgagcgtgt ggcaattccc tcggcttcgg cgcgcgcagt
     1081 caacgcggcc agggtggctg cgtccaatcg aactggcaca accactccgg gtccatcacc
     1141 caccggaggg cgaccgcgac gtggcttcgg gagcttcgcg aggtcataac cggcctcggc
     1201 ttcatcggcc cacgcctgaa tctgctcgtc agtcacaggc tgaccgtgca gcaggtgctc
     1261 actcatcctc atagcgtata cgaaaaccag tgtgcggcca acggccagct ccgttcgatc
     1321 gcccagccgt tacgacgtct tcgggctagg gtctgatttg tgactgtacg ccaagcaact
     1381 gccatcgacg ccatcgccga cgcctacacc cannnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nntacggcga gctggtgacg ttcctgaccg acgagctggc tgcgcaggcg
     1561 cccgccgacg acgccgtcgg acgcgaccgt tacgccttgt ggtcacgcga attcgtgggt
     1621 gcggccgtcg acctggacga gacctacgag tggggcctgg aagaactcga acgcatggcc
     1681 gcggagcagg accggatcgt cgccgagatc gccggcccgg gagcatcgat cgaggatgcg
     1741 atcgccaagc tcgagaccga cccggcccgc gtcctgcacg gcaaggacgc gttgcgcgaa
     1801 tggatgcagt cgaccgccga cgaggcgatt gcagcgctgg acggcgtcca cttcgacctg
     1861 gctgaaccca ttcgcaagat cgagtgcatg atcgcgccga ccgagaacgg cggcatctac
     1921 tacaccggcc cttcggacga cttctcgcgt cccggccgga tgtggtggtc ggttcccccg
     1981 ggcgtgactg aattcagcac ttggcgggag aagaccaccg tttaccacga gggcgtgccc
     2041 gggcatcacc tgcagatcgc gcaggcggta ctcaaccgct cgcaactcaa ctcgtggcgg
     2101 cgcttggtga gctggagttc tgggcacggc gagggctggg cgctctacgc cgagcggctg
     2161 atggaggagt tcgggttcat ggacgacctg ggcgaccggc tcgggctcgt cgacggccag
     2221 cgcatgcgcg ccacccgcgt ggtgctggac atcggcgtgc actgtcgcaa gcccgcgccc
     2281 gagcgctggg gcggcggcac ctgggacgcg gcgaaggcct gggagtgctt caaggccaac
     2341 gtgaacatgg acgagtcgtt cctgcgcttc gagctgaacc gctacctcgg ctggcccggg
     2401 caggcgccgt cgtacaagat cggccagcgc ctatgggaga cctatcgcgc tcaagcttcc
     2461 gcggcggccg ccgctgccgg tgaggagttc agcctgaagg acttccacac tcgcgctctg
     2521 aatcttgggt cggtgccttt ggctgtgttc ccggaggctc tgggcctgtc ctgagccgcg
     2581 gcccaccgtc atcccggcga aggccgggat ctccaagcgt ggccgtggtc ccgtggtccg
     2641 ggctgaaccc cgaggagtgc gcaggcatcg aaatgcgggt tgtgagcgcc tctgctggcc
     2701 tcagaaccct catatcgatg gtcggcgcct cagtggcgct cgcgaggagg gcttgcgagg
     2761 aacgagcgag cccgtcacga aggaccgccg cagacctcac ggcagatgcg tccgcggcgc
     2821 gagcctagga ccatgccgcg gcggggcgat cccagccatg ccgatcagcc gctgcacccg
     2881 cagccgatgc ggacggtagg gctccagcaa ctcggccaga ccggcatcat ccagcggctg
     2941 cccgacgagg gcccagccga tgtcccgggg cacgttgtag tcgccgaggc tcagcgcgtc
     3001 cgcatcaccg agggcacggg cgcgcacttc ggcgctggtc cagatgccga tgccgggcag
     3061 gctgcgcagc cgttcgtccg cgcgttcggg ctcggccggc aggacgcggt tgagcgtgtc
     3121 ggcgacccgg gcagcggtga gcagggtgcg cgagcgggcc gggtcgatgt gcatcttgag
     3181 ccagtcccag gacgggatgc ggcgcagcgc gtccgcggtc ggaaaggtgc gcagtccgag
     3241 ttgctcgccg gggccgggtg ccgggacgcc gtagcggacg agcagcgcac ggatgccgta
     3301 ggtggcttcg gcgccagtca ccttctgctc gatgacgacg gggacgaggg cgtcccaaac
     3361 cgaggcggtg cgggcgatcc gccagtgtgg gcggcttcga tgaagctctg cgagcagctg
     3421 atgcgatgga cggaagccgg acgggtcgtc cgacgcgccg agcatctgcg gcaggtcgtc
     3481 gagtgcccag gcggcgccgt caccccaggc ttcggcttcg attaccccgg acgcgtcgag
     3541 cgtccgcacc gcgagcgtgg tggtaccaac gggtgtgttt agagcgcgcc agtggcgtcc
     3601 gtcgatgacc ttgtaggtgg ggtcggccag tccgcgcttg aaggtgcccc agacctgtcc
     3661 caccgggcac ggccaagccg gacgccagct gcggtggagg ggttcggtca ctnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngatagttg tcccatgctg ctggatcagg
     3841 tggtgcagac gtcggcacgg gtcgccgcga cgagttcgcg gacggccaag gtagccgcgc
     3901 tcgcggagag cctggcagcg gcttccgctg acgaactgga gatcgtggcg cactacttgt
     3961 cgggctcgct gcggcagcgg cggacagggg tgggctggcg gtccctgctg tcgcgtccag
     4021 aggctgccga tgcgccgacg ctgacgttga cggaggtgga cgcggcgttc ggcgagttgg
     4081 ctgggttggg cggagcgggt tcacaggggg cacgacaggc atcgctggct cagctgttcg
     4141 ggcgggcgac gccggctgaa caggccttcc tgagcgggct gatcaccggg gagacgcgcc
     4201 agggggcgtt ggtcggattg ctgctgccgg cgatcgcgtc cgcgtttgcg gtgccggtgg
     4261 aggccgtgcg gcgcgcggcg atgttcgccg gctcgatccc cgtggtggcc atgaccgcgc
     4321 aggccggcgg cgaggcgggg ttggcggaga tcacgctgca ggtggggcgt ccggtgctgc
     4381 cgatgctggc gtccagcgcg ccttcgatcg aggcggcgct gggccggttt ggagcgggga
     4441 cgccggtggc ggtggaggcg aagctggacg ggattcgcat ccaggtgcat cgggagggtg
     4501 atgccgtcgg cgtctacagc cggagcctgg acgacatcac tgcgcggctg cccgcggtgg
     4561 tggccgcggt ggctcggctg cctgcgtcgc gattggtgct ggacggggag gcgctggcgt
     4621 tgcgtccgga cgggcgtccg atgctgttcc aggacacggc atcagcgcag gcggagttgg
     4681 tgccattctt cttcgacctg ctgcatgtgg acgaggtcga tctggtcgac cagccaatgt
     4741 cggtacgaca agcgcatttg gcggcattgc tgcctgcgga gctgcaagtc gcgcgtttgg
     4801 tgacgaccga gccgggtgat gcggaggact tctcgcgcca ggtgctggcg gccggacacg
     4861 aaggggtggt gatcaaggcg ctggacgccg gctaccaagc cggacggcgc ggcgcggcct
     4921 gggtgaaggt gaaaccggtg cacacgctcg acctggtggt gctggcggtc gagtggggca
     4981 gcggacggcg gcgcggactg ctgtcgaaca tccatctcgg ggcgcgcgat cccgggtccg
     5041 ggggtttcgt gatgctgggc aagacgttca agggcatgac ggacgagatg ctggcgtggc
     5101 agacggagcg cttcctggcg cttgagaccc atcgcgaggg ccacgtggtg ttcgtgcgtc
     5161 ccgagcaggt ggtcgaggtc gccttcgatg gggtgcagcg ttcgacccgc taccccggag
     5221 gcatggctct ccgcttcgca agggtggtgc gttaccggga cgataaggct gccgacgatg
     5281 ccgacacggt ggacgtcatt cgggacttgg caacacgcga accgcgctga ctcgcagacc
     5341 cgactggttc acctttcaat ggtcctagtc catggttcaa cctgtcgatg atcgagcctg
     5401 tagagattac ccgcgacgta acaccttgtc tgagcgccgc agtacagttg ggcgcgatcg
     5461 agaagcgcgg ccccaagccc tccggctggc cgcctggcaa ccgcgtcccg cacacggtgc
     5521 ccccggagga agatcagccc cggcaggagc tgtcggggaa gctgcgacct cggagatgtt
     5581 catgaccgat tcctgcgacc gctttggacc cgggcacaac gtgcactgga tccagcgtgg
     5641 acttgcctca cggcacctgt acgacgagcc accgcttcgc gccactttca tgtggcgcga
     5701 tggctgcgaa gtggacgtct ggctggagga ccacctcgag cggtggcgtt ttcacgacgg
     5761 caggcagatc accaggctcg ttgaggcgtg gaagctggac acagtcgcgt acctgctgcc
     5821 ccacggactg ttgactctgg tcggtgtcgg ctgcgtctac ccctgtcgtg acgaaagcca
     5881 gtggcgagcg tgcacctctc gccagacctg attgtgggac ggatctcgcc cgcgatgtcc
     5941 caggatgggt gggcaaggtc cgagcccgac acccgaccat ctgatccgta gtcagacgcg
     6001 ccgacccgcc cgatccgaga actcgtcgaa gacggcgagc gcctcgcgcg cgatccatac
     6061 gcgattacgt tttcgatcgc tggtctcgac cagaagaccc gcagcggtgg cttggtcgat
     6121 ggcccggcgg gcggcggtgg ctgaaacccc caaacgttcg acgacaaagg tcacgttgac
     6181 cgcgggctgc ccaaccagga ttgaggcgag acgccgaccc gctgaaccag ttcgcgaggg
     6241 cagtctcttc agccacccgt cgcggacgtc ggccaactgg tccaccaacc agcgtccgag
     6301 cccggcggcg cgaatcgacg ctgcagcgag ctggctcaca atcggttcga tgtcaccttg
     6361 ccggtaggcc gtcagcgcct tgaagtaggc gttggtgtcg gcaagcagtc cggccgatac
     6421 cgggatcgtg acccggcgaa ccaactgctt gttgcgcagg tgcgcatgca gcagcgcccg
     6481 cccggttcgt ccgttgccgt cgacgaaggg gtggatggtc
//
LOCUS       DAOPRQ010000109         6435 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_9913_length_6435_cov_13.730878, whole genome shotgun sequence.
ACCESSION   DAOPRQ010000109 DAOPRQ010000000
VERSION     DAOPRQ010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 6435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6435
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9913_length_6435_cov_13.730878"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1098
                     /locus_tag="PKJ61_11315"
     CDS             <1..1098
                     /locus_tag="PKJ61_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOC14450.1"
                     /translation="KLGLAVAAPVLAILVALVLTSIILLVAGDPVLQVWQVLFKAPLP
                     RQIVAILNEATVLYLSAVAVAIGFKMNLFNIGVDGQYRIASFAAAVFAGANLLPGWLN
                     IIVSILIAMVTGALWAAIAGLLKVTRGVSEVISTIMLNYIATYLVAYLLKGAAVKVEG
                     SNATSTAVIAESSRVPGISVESWLGTGREIYGLIFLAIAVGILFWFVLTKTRFGFDLR
                     ATGRSESAAIASGVNVKKMVVTSMILSGAIAGLVGMPLLFGQDFAYGDTVQAGLGFAG
                     MSVALLGRNHPVGMAVAALLWGYLNQLSNGLQISAGVSDKLVLIIQGIIVLSVVIAYE
                     VVRRAGLRMEQRRVARELAAQPKPATEEVAA"
     gene            1095..2363
                     /locus_tag="PKJ61_11320"
     CDS             1095..2363
                     /locus_tag="PKJ61_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007078484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOC14451.1"
                     /translation="MSTSVTAKPEPLRATLTPGRPAWYWPALIGGALVVIATIRVLTG
                     AHDIDSSGALAAAIGLAMPLALAGLGGLWSERAGVVNIGLEGMMILGTWGAAFFGFHY
                     GPWIGILGAVVMGILGGAIHALATVIFGVDHIVSGVAVNLLGAGAGAYLAVRTFTGLP
                     GGGPTQSPPLQKLPSISIPGLGDWLLGLEQSRTWLVADLAGILRTLTTDLNVLTVIAV
                     GLLVFSWWLLWRTPFGLRLRSCGEAPQAAETLGVNVIRFKFLAVLVSGALAGLAGGFL
                     VLVASNLYRDGQTGGRGYIGLAAMIFGNWRPGGLAAGALLFGYADAVQLRGRGDVIHA
                     YLLPIGLMLLAWGIWQLVRRRTGKPVGALVRVALALAVLAWYWFTDEVPAEFTNMTPY
                     IATLLVLAFSAQRLRMPAADGAIYRKGEAN"
     gene            2359..2757
                     /locus_tag="PKJ61_11325"
     CDS             2359..2757
                     /locus_tag="PKJ61_11325"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008600100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HOC14452.1"
                     /translation="MSVDWDELRSRAQGMLERAYVPYSRFPVGAAALVDDGRIVTGCN
                     VENAAYGVALCAECGLVSELVATGGGRLVAFSCCNANGELLMPCGRCRQLLWEHGGAD
                     LLVDTPLGVQSMSEVLPQAFGGADIEKVTG"
     gene            2754..3821
                     /locus_tag="PKJ61_11330"
     CDS             2754..3821
                     /locus_tag="PKJ61_11330"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020576985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HOC14453.1"
                     /translation="MSVPIEICRAVPKVALHDHLDGGLRPATIVEEAARVGHQLPTTD
                     PAELGAWFFEAADSGSLVRYLETFDHTIAVMQTHDQLRRVAAEFVEDQAADGVVYAEA
                     RWAPEQHLAGGLTLTQAVEAVRDGLAEGMAACAVAGRPIIAQQILTSMRHGMPTTEIA
                     ELAVGYRHDSVCGFDIAGAEDGFPPSRFAEAFDYLKRQNFQFTIHAGEAFGLPSIWEA
                     VQLCGASRLGHGVRLVEDIAPDGTLGDLAAYIRDRRLPLELCPTSNLQTGICERIEDH
                     PFGRLAELQFRVTVSCDNRLMSRTTLSRELALLSAAFGYTIDDLQRFTINAAKSAFLP
                     FDQRLVLIDQIKAGFAAVKAG"
     gene            complement(3808..4209)
                     /locus_tag="PKJ61_11335"
     CDS             complement(3808..4209)
                     /locus_tag="PKJ61_11335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14454.1"
                     /translation="MHASGFNLNGITTTPSGALLAVQSATGLLFRIDPRTDLGGQLLT
                     NGDGLLRQGRTLYVVQNQQNQIAVVKLSRSGATGALKRTITSPDFDVPTTVAALGKWL
                     YLPNARFGTAATPQTEYTAVHVSTRPAVSRP"
     gene            4642..4878
                     /locus_tag="PKJ61_11340"
     CDS             4642..4878
                     /locus_tag="PKJ61_11340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14455.1"
                     /translation="MSSTGRVIIVAAVRLGRTRGEAGGKSDREWSSPLLGCLKPIGRD
                     LGLDKLDQPNGANIVRTLFTDLCSGVVSAANLAT"
     gene            4888..6168
                     /locus_tag="PKJ61_11345"
     CDS             4888..6168
                     /locus_tag="PKJ61_11345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14456.1"
                     /translation="MTSNRSRSVAILADTDSRWKWAAHLAGRLHPSTPVAGFQLLGSV
                     PPSGRQLAEAGLQPGQIRSVTAAELIGALREQQPDVLILALPGGGCQAMLHLLAAAEL
                     PSRPLVVAGYVGVVYEKVVEGLLLRAGADLIVANSPADYDRFRLVLSGAGVDPSCLVL
                     GRLPFLGDARPDRLTASASDAYTVTFAGQPGVPGSLRERRYLIERLAEHARRHTARDV
                     QVKLRALPGERVTHAELYSYADLVQGLGASRPPNLKLAMGEMGEVLDRTDLLVTVSST
                     AAAEAIHRSVPTAILTDFGIRESLGNAYFLGSGCLVSFDDLDAGAAPVAEPRWARRHG
                     LGIGIDQLPGRVAEQLVRNGLPQLRPFYTLANAPALLPGLLARYGVRADGQPLSGGAA
                     GPGRLRAAVRTSARTMYRHGAEVVAPALRKLAAI"
     gene            6165..>6435
                     /locus_tag="PKJ61_11350"
     CDS             6165..>6435
                     /locus_tag="PKJ61_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase"
                     /protein_id="HOC14457.1"
                     /translation="MSGDVLVVIPARGGSKGVPGKNLARVGGVPLVVRAIRAAQMAQR
                     VTAVAMSTDDPTIAAAATAAGAIAVERPPDLAGDEATSEAALSHAV"
BASE COUNT          903 a         2147 c         2289 g         1096 t
ORIGIN      
        1 aagctcggcc tagccgtggc cgcaccggta ctggcgatcc tggtggcgct ggtgctcacc
       61 tcgatcatcc tgctggtcgc cggcgacccg gtgctgcagg tgtggcaggt gctcttcaaa
      121 gccccgctgc cgcgccagat cgtggcaatc ctgaacgaag ccaccgtgct gtacctctcg
      181 gcggtggcgg ttgcgatcgg gttcaagatg aacctgttca acatcggcgt ggacgggcag
      241 taccggatcg catccttcgc cgccgcggtg ttcgccggtg ccaacctgct gccgggctgg
      301 ctgaacatca tcgtctcgat cctcatcgcc atggtcaccg gagccctgtg ggcagcgatc
      361 gccggtctgc tgaaggtcac ccgcggggtc tccgaggtga tcagcacgat catgctcaac
      421 tacatcgcca cctacctggt cgcctacctg ctcaaaggtg cggcggtgaa ggtggaaggc
      481 tcgaacgcga ccagcactgc ggtcatcgcc gagagctcgc gagtgccggg tatcagcgtg
      541 gaaagctggc tcggcaccgg ccgggagatc tatggcctga tcttcctcgc catcgcggtc
      601 ggcatcctgt tctggttcgt gctcaccaag acccggttcg gcttcgacct gcgcgccacc
      661 ggacgctcgg agagcgcggc gatcgccagc ggcgtgaacg tcaagaagat ggtcgtcacc
      721 tcgatgatcc tctccggtgc gatcgcgggc ctggtcggca tgccgctgct cttcggccag
      781 gacttcgcct atggcgacac cgtccaggcc gggctcggct tcgccggcat gtcggtggcg
      841 ctgctcggcc gcaaccatcc ggtcggcatg gccgtcgccg cgctgttgtg gggttatctg
      901 aaccagctga gcaacggcct gcagatcagt gccggcgtct cggacaagct ggtgctgatc
      961 atccagggca tcatcgtgct gtcggtcgtg atcgcctacg aggtcgtccg tcgggccggc
     1021 ctgcgcatgg agcaacgccg ggtcgcccgc gaactcgccg cccaacccaa gccggctact
     1081 gaggaggtgg cggcatgagc acttccgtga cggcaaagcc ggaaccgctg cgcgcaacgc
     1141 tgacccccgg acgtccggcc tggtactggc cggccctgat cggcggcgcg ctggtcgtga
     1201 tcgccaccat ccgggtgctc accggcgccc atgacatcga ctcgtccggc gccttggccg
     1261 cagccatcgg gctggccatg ccgctggcgc tggccggcct gggtggcctg tggtcggagc
     1321 gggccggcgt ggtgaacatc ggcctggaag gcatgatgat cctcggcacc tggggtgcgg
     1381 cgttcttcgg cttccactac gggccgtgga tcggcatcct cggcgcggtg gtgatgggca
     1441 tcctcggcgg tgcgattcac gcgctggcca cggtgatctt cggggtcgac cacatcgtct
     1501 ccggtgtcgc ggtgaacctg ctgggcgccg gcgcgggcgc gtacctcgcc gtccgtacct
     1561 tcaccgggct gcctggcggc ggtcccaccc agtcgccgcc actgcagaag ctgccgtcca
     1621 tctcgattcc cggcctcggc gactggctgc tcgggctgga gcagtcgcgt acctggctgg
     1681 tggccgacct ggccggcatc ctgcgcacgc tgaccaccga cctgaacgtg ctcaccgtga
     1741 tcgcggtcgg cctgctggtc ttctcctggt ggctgctctg gcggacgccg ttcggcctgc
     1801 gcctgcgctc ctgcggcgag gcgccgcaag ccgccgagac gctcggcgtg aacgtgattc
     1861 ggttcaagtt cctcgcggtg ctggtgtccg gtgcgctggc gggtctggcc ggtggcttcc
     1921 tggtgctggt cgcgtccaac ctctaccgcg acggtcagac cggcggacgc ggctacatcg
     1981 gcctcgcggc gatgatcttc ggcaactggc gtccgggcgg tctggccgcc ggtgcgctgc
     2041 tcttcggcta cgcggacgcc gtccagctgc gcggccgtgg cgacgtgatc cacgcctacc
     2101 tgctgccgat cggcctgatg ctgctggcgt ggggcatctg gcaactggtc cggcggcgca
     2161 ctggcaagcc ggtgggtgcg ctggttcggg tggccctcgc cctggccgtc ttggcctggt
     2221 actggttcac cgatgaggtg ccggccgaat tcaccaacat gaccccgtac atcgcgaccc
     2281 tgctggtgct ggcgttctcg gcgcagcgat tgcgaatgcc ggccgcggac ggtgcgatct
     2341 accgaaaggg tgaggcgaat tagcgtcgat tgggacgagt tgcgctcgcg cgcccaggga
     2401 atgctcgagc gggcgtacgt gccctactcg cgcttcccgg tgggggccgc tgcgctggtc
     2461 gatgacggcc ggatcgtcac cggctgcaac gtcgagaacg cggcttacgg cgtggcgctg
     2521 tgcgcggaat gcggcctggt ctcggaactg gtcgcgaccg gtggcggacg cctggtggcg
     2581 ttctcatgct gcaacgcgaa cggcgaactg ctcatgccgt gcggacgctg ccgccagctg
     2641 ctctgggagc atggcggcgc cgacctgctc gtggacacgc cactgggggt gcagtcaatg
     2701 agcgaggtgc tgccgcaggc cttcggcggg gccgacatcg agaaggtgac cgggtgagcg
     2761 tgccgatcga gatctgccgc gccgttccga aggtggcgct gcacgaccac ctggacggtg
     2821 ggctgcgtcc ggcgacgatc gtcgaagagg cggcccgggt gggccaccaa ttacccacca
     2881 ccgacccggc cgagcttgga gcgtggttct tcgaagcagc cgactcgggt tcgctggtgc
     2941 gctacctgga gacgtttgac cacaccatcg cggtgatgca gacgcatgac cagttgcggc
     3001 gggtggccgc cgagttcgtc gaggaccagg ccgccgacgg cgtggtctac gccgaagcca
     3061 gatgggcgcc cgagcagcac ctggccggcg ggctgaccct gacccaagcg gtcgaggcgg
     3121 tccgggacgg tctggctgag ggcatggccg cgtgcgcggt cgccgggcgt ccgatcatcg
     3181 ctcagcagat cctcacttcg atgcgccacg gcatgcccac caccgagatc gccgaactgg
     3241 cggtcgggta tcgccacgat tcggtgtgtg gcttcgacat cgccggcgcc gaggacgggt
     3301 tcccgcccag ccggttcgct gaagcgttcg actatctgaa gcggcagaac ttccagttca
     3361 cgatccacgc cggcgaggcg ttcgggctgc cgtccatctg ggaggcggtg cagctgtgcg
     3421 gcgcgtcccg gcttggtcac ggcgtccggc tggtcgagga catcgcaccg gacgggacgc
     3481 tgggcgacct ggccgcctac atccgcgacc ggcggcttcc gctggaactg tgcccgacct
     3541 ccaacctgca gaccggaatc tgcgagcgga tcgaggacca tccgttcgga cggctggccg
     3601 aactgcagtt ccgggtgacc gtgagctgcg acaaccggct gatgagccgc accacgctga
     3661 gccgggaact ggccctgctc agcgcggctt tcggctacac gatcgacgac ctgcagcgct
     3721 tcacgatcaa cgcagccaag agcgcgttcc tgccgttcga ccagcggctc gtcctgatcg
     3781 accagatcaa ggcgggcttc gctgcggtca aggccggctg accgcggggc gagtcgacac
     3841 gtggacggcc gtgtactcgg tctgcggtgt cgcagcggtg ccgaaacggg cgttcggcaa
     3901 gtagagccac ttgccaaggg cagcaacggt ggtcggcacg tcgaagtccg gactggtgat
     3961 cgtccgcttg agcgctccgg tcgcgcctga gcgcgacagc ttcaccactg cgatctggtt
     4021 ctgctggttc tgcaccacgt acagggtgcg gccctggcgc agcaggccgt ccccgttggt
     4081 cagcagctgc ccgccgaggt cagtgcgcgg atcgatgcgg aagagcagcc cggtcgctga
     4141 ctgaacggcc agcaacgcac cgctcggggt ggtggtgatg ccgttgaggt tgaagccgga
     4201 cgcgtgcacg taggcgccac tgagcggcag cgtggatcac ctggtcaggg gctgagaggc
     4261 gcccccattt gccgagcggc accccgtaga gctcggcgtt ctgggagtcg gtgaaccacg
     4321 cggtgtgacg ggtgagtacc acctcgttga tgaaagctgc tccagtggtg aactgatagc
     4381 tcgccagtac ccggccggag cggacatcga ccacgcgtgc gttaccggcg gacccgccgg
     4441 cgacgaagag ccgtccgcga ccatccacct tgagccccac ggacggagtg ccggggcctt
     4501 cgctgatgac cttgccctcg ccggtggtca ggctcgcagc gtagatgtcg ccgtccgcga
     4561 gggagccgaa gtaggccgtc cgacccagga tggcgatgcc ttcgggctgg aatccgttgg
     4621 gtagctggat cgcgttgacc ggtgtcctcg accggccggg tgatcatcgt ggcggccgtg
     4681 cgcctgggcc ggaccagggg cgaggccggc ggaaagagcg atcgcgagtg gtcttccccc
     4741 ttgctcggat gcttgaaacc catcggacga gatctgggtc tcgacaagct cgaccagccg
     4801 aacggcgcca acatcgttcg gacgttgttc accgacttgt gttccggcgt tgtcagcgcg
     4861 gccaatctgg ctacctaggt tgccggtgtg accagcaatc gatcccggtc ggtggcgatc
     4921 ctcgccgata ccgacagccg ctggaagtgg gcagcccatc tggctggacg gctgcatccg
     4981 tccacgccgg tggccggctt ccagttgctc ggcagcgtgc cgccaagtgg ccggcagctt
     5041 gccgaggccg gcctgcagcc cgggcagatc aggagcgtga ctgccgccga attgatcggc
     5101 gcgctgcgcg aacagcagcc cgacgtgctg atcctggccc tgcccggtgg tgggtgccag
     5161 gcgatgctgc acctgctggc cgccgccgag ctgccgtccc gtcccctggt ggtggccggg
     5221 tacgtcggcg tggtctacga gaaggtcgtc gaagggttgc tcctgcgcgc cggggcagac
     5281 ctgatcgtcg cgaacagccc cgctgactac gaccggttcc ggctggtgct gagcggggcc
     5341 ggggtggatc cgtcctgcct ggtgctgggc cggttgccct ttctcggcga cgcccggccg
     5401 gaccggctta ctgcctcggc ctcggacgcc tatacggtca ccttcgccgg ccagcccggg
     5461 gtgccgggca gtcttcgcga gcgtcgctac ctgatcgagc ggctcgccga acatgcccgc
     5521 cgtcacactg ctcgggacgt gcaggtgaaa ctccgggcgc tgccggggga gcgggtcacc
     5581 cacgccgagc tctactccta tgccgacctg gtgcaggggc tgggcgcgtc ccgtccgccg
     5641 aacctgaagc tggcgatggg tgagatgggc gaggtgctgg atcgaaccga cctgctggtg
     5701 acggtcagtt cgaccgcggc tgccgaggcg atccatcgga gcgtcccgac ggccatcctc
     5761 actgacttcg ggatccgcga gtcgctgggc aacgcctact tcctcgggtc cggctgcctg
     5821 gtctccttcg atgacctgga cgcgggcgcc gccccggtag ccgagcccag gtgggcgcgg
     5881 cgacacgggc tgggcatcgg catcgaccag cttcccggac gggtggcaga gcaactcgtg
     5941 cgaaacggat tgccgcagct gcgaccgttc tacacgctcg cgaacgcgcc ggccctcttg
     6001 cccggactgc ttgcccgcta cggcgtccgt gccgatggcc aaccgctcag tggcggtgcg
     6061 gccgggcccg gacggctgcg cgcggcggtg cggaccagcg cccgcaccat gtaccggcac
     6121 ggcgccgaag tggtggcgcc ggcactgcgg aagctggccg caatatgagc ggcgacgtgc
     6181 tggtggtgat tccggccagg ggcggttcaa agggcgtccc aggcaagaac cttgcccggg
     6241 tcggcggggt gccactggtt gtgcgcgcga ttcgtgccgc ccaaatggcc cagcgcgtca
     6301 cagccgtcgc gatgtccacc gacgatccga cgatcgccgc ggcggccacg gcggcgggcg
     6361 cgatcgcggt ggagaggccg cccgacctcg ccggtgacga ggccaccagc gaggctgcgc
     6421 tgagccatgc cgtcg
//
LOCUS       DAOPRQ010000110         5983 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_10825_length_5983_cov_12.264507, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000110 DAOPRQ010000000
VERSION     DAOPRQ010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 5983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5983
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10825_length_5983_cov_12.264507"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(248..2683)
                     /locus_tag="PKJ61_11355"
     CDS             complement(248..2683)
                     /locus_tag="PKJ61_11355"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOC14458.1"
                     /translation="MLKQALAEVRLHPGRFVSTILAVAISVAFLAGSSVLVATEGRAE
                     GMSTNLGISAADLVVTSEEALDLRPIVAQTPGLTAAHPVLQLTEPLRGERESVLVGLY
                     LVPAEQLRWANLTAGRWPEAADEIALSRGGASALGVTVGAVVTAAGDERVTVVGVTDE
                     PGSAFVRTGYAAADRFVRNGLVTDAVPGQWAVRAQPGADLAALAQVLGSKLEEYGPTV
                     VVRPAADVRAEALKQSTRGVEVFKYLLWTFAVVALVVGMITVANTFAITLAQRRRQIG
                     LLRAVGASGAQVRRRFLAEAVILGVLGSLVGVAAGIGLAALAAAYTGSLFWGLEFPSA
                     ELWLAFGAGVASTTAAAWLPIIRGTRVHPLEALQPALTGEEQRRASIVRGLICGLLLA
                     GGAALVALALAGRENAFLEAIAAGALIALGVLFGAPLFVPGLLRSTGTLVGRWGTVAS
                     LAAKNSERNPRRATATATALMLAVGLIVTLQVGTASARETMLAEINRTKPVDIAVTWQ
                     DADGGAAAIPEAKRTALAMAPGVTDSLALAAVTALLTDQESAQEVTVLGYDPAIAGLT
                     GVPDGVGDDEILVGANQAEYLADVVRVKGPKGERELRVVASKLADYDSALVSAGTLAE
                     LGTPIAGAVMWLSVPERSAAVDALLQVHELAGDSAQVGGSVVEAAQVEQVLDLLLAIT
                     TALLGVAVLIALIGVSNTLGLSVLERTRESALLRALGLQARSLRLMLTVEAMQVTLVG
                     VLVGLVSGTFFGWLGISSLGRTMRVQEVRFAVNVPQTLGMLALAVLAAALASVLPGRR
                     AAKASPTEALADI"
     gene            complement(2687..3436)
                     /locus_tag="PKJ61_11360"
     CDS             complement(2687..3436)
                     /locus_tag="PKJ61_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016666644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOC14459.1"
                     /translation="MTLPTHLQSEPIAVATDLVKTYGSGDAAVRALDGVTVAFAKGSF
                     TAIMGPSGSGKSTLMHCLAGLDRPTAGEVTLAGRGLANLSDRELTAVRRDHVGFVFQA
                     FNLLPTLTAAQNIVLPLELAGRRPDASALADIVESLGLTDRLSHLPSQLSGGQQQRVA
                     IARALITEPSVVFADEPTGALDSRSGHHLLGYLRAACRNLGQTIVMVTHDPNAAAYAD
                     RTVLLSDGRIVDDLSSPDADAILAAMKLLGA"
     gene            3638..5011
                     /locus_tag="PKJ61_11365"
     CDS             3638..5011
                     /locus_tag="PKJ61_11365"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002528923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HOC14460.1"
                     /translation="MALSVFDLFRVGIGPSSSHTVGPMKAAGRFVQALAASGALAEVE
                     KVRAELFGSLGATGHGHGSVPAVVLGLAGHQPDTVDPPAVRPLVAQIRAEHRLALANG
                     PTIDFDPDADVVLHRNLSLPVHANGMTFTAWDASGAELDRRTYYSVGGGFVLDESETG
                     PDRIVPDATVVPHPFGSGAELLAHCGETGFSIAGLMRENERTWRTEADIDAGLLHLWA
                     VMQECIDHGMTTEGVLPGGLKVRRRAPELLQRLQAEADSTDPLRGMDWITLYALAVNE
                     ENAAGGRVVTAPTNGAAGVVPAVLMYYRRFVHGSDDEGVIRFLLTAGAIGVVIRELAS
                     ISGAEVGCQGEIGAACSMAAAGMAAALGGTAGQVENAAEIGMEHNLGLTCDPVGGLVQ
                     IPCIERNAIAAVKAVTAARTALRGDGRHIVSLDSVLKTMRQTGADMSVKYKETARGGL
                     AVNVIEC"
     gene            5085..>5983
                     /locus_tag="PKJ61_11370"
     CDS             5085..>5983
                     /locus_tag="PKJ61_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013162219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOC14461.1"
                     /translation="MPILDLYTPDTDRYDGRMPYRPVGRTGLKLPAISLGLWHNFGDD
                     TPFANQRATLRRAFELGISHFDLANNYGPPYGSAETNFGRHLREDFKPYRDELIISSK
                     AGWDMWPGPYGDLGSRKYLLASLDQSLQRMGLDYVDIFYHHHYDPTTPLAETMLALDT
                     AVRQGKALYVGISSYSAERTREAVAIATELGTPLVIHQPSYSMLNRWVEDATAGESLL
                     DALGDTGLGCIAFSPLAQGMLTDKYLNGVPEGSRAAQDKSLSTDLLSAQNLDHIRALN
                     DLAAGRGQTLAQLAIAWVLRDERV"
BASE COUNT          962 a         2143 c         2028 g          850 t
ORIGIN      
        1 ccggcgaaca cctcgtcgcg gctctccctg cgcgcccaga tcaaccccgc gacgcccagc
       61 gcgagcagca ccacaccgat gaagagttcc atccgcctcc gtccgttgtg gacattctgc
      121 cggtttgggg aaggcggcag cccaacgtgg cgcgttgcgg gtcctgagga acacgagtga
      181 gcttgcgaac tcgtgtgtca cgaagggcgt tcctcacaac gctcctcagg gaccggttgc
      241 gaggagctca gatgtcggcc aaggcctccg tcggggacgc cttcgcggct cgccgcccgg
      301 gcagcacgga cgccagcgcg gcggccaaca ccgcaagggc caacatgccc agcgtctgcg
      361 gcacgttcac tgcgaaccgc acttcctgga cgcgcatcgt gcggcccagc gacgaaatgc
      421 ccagccagcc gaagaaagtg cccgagacca gccccaccag caccccgacc agggtcacct
      481 gcatcgcctc caccgtgagc atcaggcgga gcgaccgcgc ctgcaacccc agcgcccgca
      541 gcaacgccga ctcccgggtc cgttcgagca ccgacagccc cagcgtgttg ctgacgccga
      601 tcagcgcgat cagcaccgcc actcccagca gcgcggtggt gatcgccagc agcaggtcga
      661 gcacctgctc cacctgggcc gcctcgacca ccgagccacc gacctgggcc gagtcgccgg
      721 ccaactcgtg cacctgcagc agcgcgtcca cagccgctga ccgttccggc accgacagcc
      781 acatcaccgc gcccgcgatc ggtgttccca gctcagctag ggtgcccgcc gacaccaacg
      841 cactgtcgta gtcggccagc ttggacgcca ccacgcgcag ctcgcgctcc cccttcggac
      901 ccttgacccg caccacgtca gccaggtact cagcctggtt ggcgccgacc aggatctcgt
      961 cgtcgcccac accgtcaggc acgccggtga gaccggcgat cgccgggtcg tagcccagca
     1021 cggtcacctc ctgggccgat tcctggtcgg tcagcagtgc ggtcacggcc gccagcgcca
     1081 gcgagtccgt cacccccgga gccatcgcca gagccgtgcg cttggcttcg gggatggcag
     1141 ccgcaccgcc gtccgcgtcc tgccaggtca ccgcgatgtc caccggtttg gtgcggttga
     1201 tctcggcgag catcgtctcg cgggcgctcg ccgtgccgac ctggagcgtg acgatcagcc
     1261 cgaccgccag catcagcgcg gtcgcggtcg cggtggctcg gcgcgggttc cgctccgagt
     1321 tcttcgccgc cagggacgcg accgtccccc accggcccac cagcgtgccg gtcgagcgca
     1381 gcaggccggg cacgaacagc ggcgccccga acagcacgcc gagcgcgatc agtgcaccgg
     1441 ccgcgatcgc ttcgaggaag gcgttctcac gtccggcaag ggcgagcgcg accagcgccg
     1501 caccgccggc cagcaatagt ccgcagatca gcccgcgcac gatggacgcg cgccgctgct
     1561 cctcgccggt gagggccggc tgcagcgcct ccaaggggtg cacccgcgtg ccccggatga
     1621 tcggcagcca cgccgctgcg gtggtggacg cgacgccggc cccgaacgcg agccacaact
     1681 ccgcggacgg gaactccagg ccccagaaca gcgagccggt gtaggcggcg gcgagcgcgg
     1741 ccaagccgat gcctgctgcc acccccacca gggagccgag cacgcccagg atcaccgctt
     1801 cggcgaggaa ccgccgccgc acctgggcgc cgctcgcgcc gaccgcacgc agcaggccga
     1861 tctgtcggcg ccgctgcgcg agggtgatcg cgaaggtgtt cgccaccgtg atcatcccaa
     1921 ccaccagcgc caccacggcg aacgtccaca acaggtactt gaatacctcc acgcctcggg
     1981 tgctctgctt gagggcctcg gcccggacgt cggccgcagg gcgcaccacg accgtgggcc
     2041 cgtactcctc cagcttgctc cccagcacct gggcgagcgc ggccaggtcg gcgcccggct
     2101 gagcccgcac cgcccactgc cccggcaccg cgtcggtaac caagccgttg cggacgaagc
     2161 gatcagccgc tgcgtagccg gtgcggacga acgccgatcc gggctcatcg gtgactccca
     2221 ccacggtcac ccgctcgtcg ccggctgcgg tgacgaccgc gccgacggtc acccccagtg
     2281 cggacgcgcc gccccggctg agcgcgatct cgtccgcagc ctcgggccag cgtccggcgg
     2341 tcaggttcgc ccagcgaagc tgctcggcgg ggacgaggta cagcccgacg agcaccgatt
     2401 cccgttcgcc acgcagtggc tcggtgagct gcagcaccgg gtgcgcggcg gtaaggccag
     2461 gggtctgggc cacgatcgga cgcaggtcga gtgcctcctc gctggtgacg acgaggtcgg
     2521 ccgcggagat ccccagattc gtcgacattc cttccgcgcg accctcggtc gcaaccagga
     2581 ccgatgagcc ggcaaggaag gccacgctga tcgcgaccgc gaggatggtc gacacgaacc
     2641 gtcccgggtg caggcgcacc tctgccaagg cctgtttgag catggttcag gcccccagca
     2701 gcttcattgc ggccaggatc gcgtctgcgt ccgggctact caggtcgtcg acgatccggc
     2761 cgtcgctgag caggaccgtc cgatcggcgt aggcggcggc gttcgggtcg tgcgtgacca
     2821 tcacgatcgt ctggcccagg ttgcggcacg ccgcccgcag gtagcccagc aggtggtgcc
     2881 ccgaccgcga gtcgagcgct ccggtcggct catcggcgaa gaccaccgat ggctcggtga
     2941 tcagcgccct tgcgatggcg acccgctgct gctgcccacc tgagagctgg gacggcaggt
     3001 ggctgagccg atcggtaagg cccagcgact cgacgatgtc ggcgagcgcg gaagcgtccg
     3061 gacggcggcc ggcaagctcc aggggcagca cgatgttctg agccgcggtg agggtgggca
     3121 gcaggttgaa ggcctggaag acgaagccca cgtgatcgcg gcggacggcg gtgagttcac
     3181 ggtcggaaag gttcgcgagc ccgcgaccgg ccagcgtcac ctcgcctgcg gtcggccggt
     3241 cgagcccggc caggcagtgc atcagcgtgg acttgcccga gccggacggc cccatgatcg
     3301 cggtgaacga ccccttcgcg aatgcgaccg tcaccccgtc gagggctcgc acggcggcgt
     3361 ccccgctgcc gtaggtcttg accaggtcgg tcgcgaccgc gatcggttcg gactgcaggt
     3421 gggtgggcag ggtcatcggg gcagaggtag tcacagtgcg agtctggctg gcgggtcacc
     3481 ccgcggtcgt cctccccagg gtcggtccga ctcgtccgat cggacgatct ggagccatga
     3541 gtcgacgagc ccgatcgagc ggtccagctg gtcgagcggt ccagctggtc gagcccgcca
     3601 ccatccgaac ccgacccaac cgagctatgg tcaaggcatg gcgctcagcg tcttcgacct
     3661 gttccgggtg ggcatcggcc cgtcctcttc gcacacggtc ggcccaatga aggccgccgg
     3721 gcggttcgtg caagcgctgg ccgcgtccgg ggcactggcc gaggtggaga aggtgcgggc
     3781 cgagttgttc ggctcgctgg gcgcgaccgg ccacggccac ggcagcgtcc cagccgtcgt
     3841 gctggggctg gccggccacc agcccgatac ggtcgacccg cccgcggtgc gtccgctggt
     3901 tgcacagatc cgtgcggagc atcggctcgc tctcgccaac gggcccacga tcgatttcga
     3961 cccggacgcg gacgtggtgc tgcaccgcaa cctttcgctg ccggtgcatg ccaacggaat
     4021 gaccttcacc gcctgggacg cgtccggagc cgagctggac cgacgcacct actactcggt
     4081 ggggggcggg ttcgtgctgg acgagtccga gaccggtcca gacaggatcg tgccggacgc
     4141 gaccgtggtg ccgcacccgt tcggcagcgg cgcggagctg ttggcgcatt gcggggagac
     4201 cgggttctcg atcgcaggat tgatgcgcga gaacgagcgg acgtggcgaa ccgaagccga
     4261 catcgacgcc ggcctgttgc acctctgggc ggtgatgcag gagtgcatcg accacggcat
     4321 gaccaccgag ggtgtgctgc cgggcgggct gaaggtgcgc cggcgcgcgc ccgagctgct
     4381 gcagcgcctg caggcggagg cggacagcac cgatccgctt cgcggaatgg actggatcac
     4441 gctctacgcc ctggccgtga acgaggagaa tgctgccggc ggacgggtgg tcaccgctcc
     4501 gaccaatggt gccgctggtg tagtaccggc agtactgatg tactaccgac gcttcgttca
     4561 cggttcggac gacgagggcg tgatccggtt cctcttgacc gctggcgcga tcggcgtggt
     4621 gatcagggaa ctcgcctcca tcagcggagc ggaggtgggg tgccaaggtg agatcggcgc
     4681 tgcctgttcg atggccgctg cgggcatggc ggcagccttg ggcggcaccg ctgggcaagt
     4741 ggagaacgct gccgagatcg gtatggagca caacctcggg ctcacctgcg atccggtcgg
     4801 cgggttggtg cagatcccct gcatcgagcg caacgccatc gccgcggtga aggccgtcac
     4861 ggccgctcgc acagccctgc gtggtgacgg acggcacatc gtcagcctcg actcggtgct
     4921 gaagaccatg cgccagaccg gagccgacat gagcgtgaag tacaaggaga ccgcccgcgg
     4981 cggcctggct gtgaacgtca tcgagtgctg atgtgggtgg ccgtaggtcc cggcaaccgc
     5041 tcagcagcgg ataagttgag aacctcacga aagaggtgcc cagcatgccg atccttgatc
     5101 tctacacccc tgacaccgac cgttacgacg gccgaatgcc ctaccgcccc gtcggacgca
     5161 ccggactgaa gctgcccgcg atctcgctgg gcctttggca caacttcggc gacgacaccc
     5221 cgttcgccaa ccagcgcgcc accctgcggc gggcgttcga actcggcatc agccatttcg
     5281 acctggccaa caactacggt ccgccctatg gctcggcgga gaccaacttc ggccggcacc
     5341 tgcgcgagga cttcaagccc taccgcgacg agttgatcat ctccagcaag gccggctggg
     5401 acatgtggcc cggaccctac ggtgacctgg gctcccgcaa gtacctgctc gcgtccctcg
     5461 accagtcgct gcagcggatg ggcctggact acgtcgacat cttctaccac caccactacg
     5521 accccacgac gccgctggcc gagacgatgc tggcgctgga taccgccgtc cgccagggca
     5581 aggcgctcta cgtcggcatc tcctcctact cggccgagcg gacgcgggaa gcggtcgcga
     5641 tcgcgaccga gctcgggacg ccgctggtga tccaccagcc ctcgtactcg atgctgaacc
     5701 gctgggtcga ggacgcgacc gcgggcgaga gcctgctgga tgcacttggt gacacggggt
     5761 tgggttgcat cgcgttctcc ccgctggccc agggcatgct caccgacaag tacctcaacg
     5821 gcgttccgga gggctcgcgg gcagcgcagg acaagtcgtt gtccaccgac ctgctctctg
     5881 cgcagaacct ggaccacatc cgcgcgctga acgacctggc cgccggacgc ggtcagaccc
     5941 tggcccagct ggcgatcgcc tgggtgctgc gggatgagcg ggt
//
LOCUS       DAOPRQ010000111         5980 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_10832_length_5980_cov_12.126582, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000111 DAOPRQ010000000
VERSION     DAOPRQ010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 5980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5980
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10832_length_5980_cov_12.126582"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /locus_tag="PKJ61_11375"
     CDS             complement(<1..713)
                     /locus_tag="PKJ61_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014847487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOC14462.1"
                     /translation="MIAVTDLKKVYRQANRDIHALDGVTLTVPENSVHGVIGQSGAGK
                     STLVRCLALLDRPTSGVVEINGVELTAVPSAELRRARRRLGVVFQHANLFDSRTITAN
                     VAYPLELAGVGRAARTAKATELLGLVGLAEAGDAHPAQLSGGQRQRVGIARALATDPD
                     VLLCDEPTSALDPATTGEILDLISALKVRLGLAVLVITHEMNVVKRICDSVSLLEDGR
                     VVESGPLTEVVGTFGSRLS"
     gene            1395..2033
                     /locus_tag="PKJ61_11380"
     CDS             1395..2033
                     /locus_tag="PKJ61_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017887769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOC14463.1"
                     /translation="MDETSARSPGRYPKGVARRRQILEMAIAQFADRGVDAASLRSIG
                     EAIGVSHTALRHYFSSRDELLIEVYRTHESLQHYTPTAGDTQDAAAAISASAEANRAV
                     PGLVQLYSTLSADALGEQHPITREFISARFDQLRSALAESVREHQAAGELRADVDADE
                     VAALVIAASDGLQIQWMLAPDTVDVARCLDLVSKLLAPTSPQDAEGRDGGPS"
     gene            2172..4415
                     /locus_tag="PKJ61_11385"
     CDS             2172..4415
                     /locus_tag="PKJ61_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HOC14464.1"
                     /translation="MVGNQDGSRVEALLSQLSLKEKVGQLIQYFYFQLPEQPAADGAI
                     QFDMSTQPRGVEAALSEGGVSSLLFVTRPEVINRLQRLAIEGSAHGIPAIFGFDVIHG
                     LRTILPVPIALAATWDAGVIERSQAVAAREARAVGIHWTFAPMVDIARDPRWGRMIEG
                     AGEDPLLGSMVAAAQVRGFQGDRLGASEHIIAGPKHYAGYGAALGGRDYDEVDISDQE
                     FWNVYLPPFAAAVKAGAGNIMSAYMDLNGVPATGNKWLLDDVLRGELGFEGFVVSDAN
                     SVKDLETHHFAADATDAAVRALTAGLDLEMAVTDAAYANLVEAVESGRVPIELVDRSV
                     RRVLLAKEAIGLFDQPYVDEDAAAQVLAAPEHREAARLAAEASAVLLRNEGSLLPLSP
                     ALARVAVIGGLAASKRDTLGPWVFDYDLDETVTLLDGIRNHLGDSVQVDYAQGAATPT
                     RLYPSMFDMFGDGTGIVDPDFDADAEFDRAVQAAAAAEVAIVVVGENQDMIGEAASRS
                     SLDLPGRQLELLQAVVATGTPTVLLVMNGRPLDLSWAAENVPAILDIWYPGTQGGAAV
                     ANLLFGAAEPRGRLPFTWPRSIGQVPMHHAVTRGHSPQNQQMRYWNEEHTPLFPFGFG
                     LGYASFSYGEVSVTPASVPVNGEVEVAVDVTNTSERAGQANVQLYLHQRSGSSSRPLR
                     QLKGFQLVELEAGETKRVVVKVGPDERRYWSSATHGYVLDESIFDVWVGADATADASA
                     TFTVSGN"
     assembly_gap    4463..4562
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4563..4822)
                     /locus_tag="PKJ61_11390"
     CDS             complement(<4563..4822)
                     /locus_tag="PKJ61_11390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14465.1"
                     /translation="MSETAVRRPLLSRLDPADVVDVFVYVVVLNLAAEYLPMVISETF
                     TLSLLTAILLKLVLELVVWLKDRAKLRLKSATTAVGKVTSVL"
     gene            complement(4909..5826)
                     /locus_tag="PKJ61_11395"
     CDS             complement(4909..5826)
                     /locus_tag="PKJ61_11395"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOC14466.1"
                     /translation="MKLGFFTACLPGVPLADVASWAAATGYEALEVACWPVSPDRPFT
                     ASHLDVTADADRLAEAASVVADHGLTISSLAFYGNNLDPDPAERAATHAHVERCLEAA
                     AVLGVPTVGTFVGRDWTRSVRQNLDTAEEVFKPLVDRAGELGVKIVIENCVMEGWHPD
                     GYPGNLAYSPELWEWMFDQGLYLNYDPSHLVWMGIDPVAALRPYVGRIAHVQAKDVQL
                     FPEKRNRYGWTGKAVHRDNPWDVGWWRYRVPGLGEVDWRGVVDALYEGGFTGAVSVEH
                     EDPLWGGTEAKVKTGLEIAYRTLRPLIVE"
BASE COUNT          955 a         1974 c         2003 g          948 t
ORIGIN      
        1 tcgctcagcc ggctgccgaa agtgccgacc acttcggtca gcggaccgga ttcgacgacc
       61 cgtccgtctt cgagcagcga gacggagtcg cagatgcgct tgaccacgtt catctcgtgg
      121 gtgatcacca gcacggccag cccgagccgg accttgagcg cgctgatcag gtcgaggatc
      181 tcgccggtgg tggccggatc gagcgcactg gtcggctcgt cgcagagcag cacgtccggg
      241 tcggtggcca aggcgcgggc gatgccgacc cgctgccgct gcccacccga gagctgcgcc
      301 gggtgcgcgt cgccggcttc ggccaggccc accagcccaa gcagttcggt ggctttggcc
      361 gtccgcgcgg cgcgtccgac cccggccagt tcgagtgggt aggcgacgtt ggccgtgatc
      421 gtgcgggagt cgaagaggtt ggcgtgctgg aagaccacgc cgagccgccg gcgggctcgc
      481 cgcagctcag cggacgggac ggcggtcagt tcgacaccgt tgatctcgac cacaccggac
      541 gtcggccggt cgagcagtgc caggcagcgc accagggtgg acttgccggc gccggattgt
      601 ccgatgacgc cgtgcacgga gttctccggc accgtaaggg tgacgccgtc gagcgcgtgg
      661 atgtcccgat tcgcttggcg gtagaccttc ttcaggtcgg tgactgcgat cactttgggt
      721 ctcttctgtc gggctgcggc agcgcgcgtc cgcaacagga cggcgcatcg gcgcgggttt
      781 gaagtggccc gacagggcag gacagcgtcg ctgtggacgc ttcagtctga gatcaggtca
      841 gcaggaccga tcggaatgcg ctgtcgggtc ggaggatcaa cgacaacaca ttcgcgcgca
      901 catcatggcc ggcgtcacgg gacgcaggct ggcatgacgg cacacgatgt tgaacacgca
      961 gccaagctag cccgcgtcct cctgcgcgcg caaatcggtc ggaccgtttc gttgaaaccc
     1021 ggctcagcct cagccatgac gcgatttcga ccggcgatcc tgcggcaacg ccgccagggg
     1081 cgaccctggc ggccgctgcg gtccgaatcg catcatcgcg ggccggccgg gatcccgcgg
     1141 tgtcgcgatc tcggcaatcg gtgaccaaga tgagccctgg atccgcccca tccgacccga
     1201 gagctacggg atcgcgacac cgccaccgtc cgagccccgg tacgccgctg cccaagacgc
     1261 gatttcgacc cgcgatcttc cgcaacgccg ctagggcggg cggtcgctgc ggtccggatc
     1321 gcgtcgtgcg gtcatcccgg gtcggctggt ggggctgctg agacacaacc agccacgatc
     1381 cgtacgatga gcgcgtggac gagacctctg cccgctcccc cgggcgttat ccgaagggag
     1441 tagcgcggcg tcgccagatc ctcgagatgg cgatcgcgca attcgccgat cgcggcgtgg
     1501 acgcggcgtc gctgcgtagc atcggtgagg cgatcggggt ttcgcacact gcgctgcggc
     1561 actacttctc ctctcgcgac gaactgctga tcgaggtcta tcggacccat gagtcgctgc
     1621 agcactacac tcccaccgct ggtgacacgc aggatgcggc cgcggcgatc agcgcgtccg
     1681 ccgaggccaa ccgggccgta ccggggttgg tgcagctcta ctccaccttg tccgcggacg
     1741 cactgggcga gcagcacccg atcacgcgtg aattcatctc tgctcggttc gatcagttgc
     1801 gcagcgcgct ggcggaatcc gttcgcgagc atcaggcggc cggcgaactg cgcgccgatg
     1861 tggacgccga cgaagtggcc gcgctggtga tcgccgcgtc cgacggcttg cagatccagt
     1921 ggatgctggc ccccgacacc gttgatgtcg cccgctgcct cgacctggtg agcaagttgc
     1981 tggcgccgac ctcgccccaa gacgcggagg gacgtgacgg cggcccttcg tgacgcccag
     2041 tcctcaggga ccggttcgcg tggcggtggc tgcaactgcc accttcagaa tcaggcgtcc
     2101 gactcttgcc aactgcaccc gcaggatgaa aagcttccac tggaacaatt cttgccgcga
     2161 ggaggctgca aatggttggg aatcaggacg ggtcaagggt cgaggcgctg ctctcgcagc
     2221 tgagcctgaa ggagaaggtc ggccagctga tccagtattt ctacttccag ctgcctgagc
     2281 agcccgctgc ggacggcgca atccagttcg acatgtccac gcagccccgc ggcgtcgaag
     2341 ccgcgctcag cgagggtggc gtcagctcgc tgctgttcgt gaccaggccc gaggtgatca
     2401 atcgcctgca gcggctcgcg atcgagggca gtgcacacgg catcccagcg atcttcggct
     2461 tcgacgtgat tcacggcctg cgcactatcc ttcccgtccc gatcgcactg gccgccacct
     2521 gggacgcagg cgtgatcgag cgttcccagg ccgtggctgc gcgcgaggcc cgcgcggtcg
     2581 gcatccactg gacgttcgcg cccatggtcg acattgcccg tgacccccgc tggggccgga
     2641 tgatcgaggg cgcgggcgaa gatccgctcc tcggctcgat ggtcgcggca gcccaggtgc
     2701 gcgggttcca gggcgaccgg ctcggcgcgt ccgagcacat catcgccggc ccgaagcact
     2761 acgccggcta tggcgcggct ctcggcggac gtgactacga cgaggtcgac atctccgacc
     2821 aggagttctg gaacgtctac ctgcctccct ttgccgccgc ggtgaaggcc ggcgccggca
     2881 acatcatgag cgcctacatg gacctcaacg gcgtccccgc gaccggcaac aagtggctgc
     2941 tcgacgatgt gctgcgcggc gagctcggct tcgaaggctt cgtggtcagc gacgcgaact
     3001 cggtcaagga tctggagacc caccacttcg cagcggacgc gaccgacgcc gccgtccgcg
     3061 ccctgaccgc cggtctcgac ctggagatgg cggttacgga cgcggcctac gcgaacctgg
     3121 tcgaagccgt cgaaagcgga cgggtgccga tcgagctggt cgatcgcagc gtgcgccggg
     3181 tgctgctggc gaaggaggcg atcggcctgt tcgaccagcc gtacgtcgac gaggacgccg
     3241 cggcgcaggt gctggcggca ccggagcacc gcgaggctgc ccggctcgcc gccgaggcca
     3301 gtgcggtgct gctgcgcaac gaaggctcgc tgctgccgct gtcccccgca ctggcgcggg
     3361 tcgcggtgat cggtgggctg gccgcttcca agcgcgacac gctcgggccg tgggttttcg
     3421 actacgacct cgacgagacc gtcaccttgc tggacggaat ccggaaccac ctcggtgatt
     3481 cggtgcaggt cgactacgcc cagggcgcgg cgacgccgac ccggctctat ccgtccatgt
     3541 tcgacatgtt cggcgacggc accggcatcg tcgacccgga cttcgacgcc gacgccgagt
     3601 tcgatcgggc cgtgcaggcg gccgccgctg ccgaggtggc gatcgtcgtg gtcggcgaga
     3661 atcaggacat gatcggcgag gccgcgtcgc gttcgtccct cgacctgccc ggacgccagc
     3721 tcgagctgct gcaggctgtg gtggcgaccg gcaccccgac ggtgctgctg gtgatgaacg
     3781 gtcgtccgct cgacctgagc tgggcggccg agaacgtgcc cgcgatcctc gacatctggt
     3841 accccggcac gcagggcggc gcagcggtcg ccaacctgct tttcggtgcc gccgagccgc
     3901 gcgggcgcct acccttcacc tggccgcgca gcatcggtca ggtaccgatg caccatgcgg
     3961 taacccgtgg acacagtcca cagaaccagc agatgcgcta ctggaacgag gagcacaccc
     4021 cgctgttccc gttcggcttc gggctgggat acgcgtcgtt cagctatggc gaggtgtcgg
     4081 tcacccctgc gtccgtgccg gtgaacggcg aggtcgaggt ggccgtggac gtcaccaaca
     4141 cctcggagcg ggccggccag gcgaacgtgc agctgtacct acaccagcgc tccggcagct
     4201 catcgcgtcc gctgcggcag ttgaagggct tccagctcgt ggagctggaa gccggcgaga
     4261 ccaagcgggt ggtggtcaag gttggcccgg acgagcgccg ctactggagt tcggccaccc
     4321 acggctacgt gctggacgag tcgatcttcg acgtgtgggt cggcgcggac gccaccgcgg
     4381 acgcgtccgc taccttcacg gtcagcggca actgagccac ccgaaggtag cagcaagctc
     4441 gatcagccgg ggtgatcctc acnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnatgagcac cgaggtgacc ttgccgacgg ccgtcgtcgc cgacttcagg cgcaacttgg
     4621 cgcgatcctt gagccagacc accagttcga gcaccagctt cagcaggatg gcggtgagca
     4681 gcgacagggt gaacgtctcc gagatcacca tgggcaggta ctcggcggca aggttgagca
     4741 cgaccacata gacgaagaca tcaaccacgt ccgccggatc cagccggctg agcagcggtc
     4801 tccggacggc agtttcgctc acgtcttccc ccgtttggct gcgatgtcac cgatcaccgt
     4861 taccgagccg gacgcccgcg tcaagtgagc gcccgtcaga ggcgagcgct actcgacaat
     4921 cagcggacgc agcgttcggt aggcgatctc caagcccgtc ttgaccttgg cctcggtacc
     4981 tccccagagc ggatcctcgt gctccaccga gaccgcgccg gtgaaaccgc cttcgtagag
     5041 cgcatccaca acgcccctcc agtcgacctc gccgagaccc ggcacacggt agcgccacca
     5101 gcccacgtcc caggggttgt cgcgatggac cgccttgccg gtccagccat aacgattgcg
     5161 cttctccgga aagagctgaa cgtccttggc ctgcacgtgg gcgatccggc cgacgtacgg
     5221 gcggagcgct gcgaccgggt cgatgcccat ccacaccagg tgggacgggt cgtagttgag
     5281 gtacaacccc tggtcgaaca tccactccca cagctcgggc gagtaggcca gatttcccgg
     5341 gtagccgtcg ggatgccaac cctccatcac gcagttctcg atgacgatct tcacgccgag
     5401 ctcaccggcc cgatcgacca acggcttgaa cacttcctcg gcggtatcga ggttctgccg
     5461 gactgaacgc gtccagtcgc ggccgacgaa ggttccaacg gtgggcacgc cgaggacggc
     5521 ggcggcttcg aggcagcgct cgacgtgggc gtgtgtggcg gcacgctctg cggggtccgg
     5581 gtcgaggttg ttgccataga aggccagcga ggagatcgtc aggccgtggt cggcgacgac
     5641 ggacgcggct tcggcgagac ggtcggcgtc cgcggtcacg tcgaggtgcg aggcggtgaa
     5701 cggccggtcc ggtgagactg gccagcaggc cacttccagc gcctcatagc cagtggctgc
     5761 agcccaactc gcgacgtccg cgagcgggac gccgggcagg caggcggtga agaatccgag
     5821 cttcaagatc ggtacctctc tcgtgcttgt ccgacgaagc tatcagctat ctaccccagc
     5881 ggagccgtgc cgcggtatcg gtggcgggtg tgggtaatgg ccggtcatgg ttttttaatc
     5941 gaccgggtca cgacctgcct gtgccgtcgt tgggccgctg
//
LOCUS       DAOPRQ010000112         5900 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_11020_length_5900_cov_12.252866, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000112 DAOPRQ010000000
VERSION     DAOPRQ010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 5900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5900
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11020_length_5900_cov_12.252866"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="PKJ61_11400"
     CDS             <1..372
                     /locus_tag="PKJ61_11400"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOC14467.1"
                     /translation="QTGQYWSGVTGVSMGSLVGAVVFLVPGVLAGGASLWQPQVLLLG
                     SVVAVMSSVIPYGLEMVALRSLPSGLFGILMSLEPAAAALAALVVLKEQLSMVEIVAM
                     ACVIIASVGATRTLAKAPAPN"
     gene            complement(380..670)
                     /locus_tag="PKJ61_11405"
     CDS             complement(380..670)
                     /locus_tag="PKJ61_11405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14468.1"
                     /translation="MDSSGSTFDGTTWHPPQRAVGPAAPSTPVPGYAFTDPHYGYGLT
                     RQTSDDLQFIARLTRVMIWIWISLVILAIVPTMILMMQLFALLAALAAWIPR"
     gene            complement(848..2533)
                     /locus_tag="PKJ61_11410"
     CDS             complement(848..2533)
                     /locus_tag="PKJ61_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HOC14469.1"
                     /translation="MAPWWHSAVVYQIYPRSFADSNADGIGDLPGIIAHLDHLAALGV
                     DVVWLSPVYRSPMADNGYDISDYDDVDPLFGTLEDLDALIAGLHDRGMKLVMDLVVNH
                     TSDEHPWFVESRDPASDKRDWYFWRPARPGFEPGTPGAEPTNWGSFFSGRGWQFDPAS
                     GEYYLHLFAPKQPDLNWENPAVRAEIYAMMRRWVDRGVDGFRMDVINLISKTLPLADG
                     PAAPDGICYDPTLVVEGPRLHEFLRELNREVGISAKELFSVGEMVLVDVETARSHTDP
                     AAGELGMVFTFEHMSVDAGPSGLKWELVPLQLPALKANLAKWQDGLADVGWNSLYFEN
                     HDQPRSVSRFGDDSPEFRVASAKTLATTLHLLKGTPYVYQGQELGMTNAGFSAIEHYD
                     DIESVNFYAQAVASGADPNQLLPVIAFKSRDNARTPMQWDASDTAGFTDGTPWLPVTA
                     NHAEINAAAQVGDPGSVFNHYRALIGLRHELEVVQHGRFQLLLGDHEQLFCFTRTLGE
                     ERLLVLANWSSAPVALPSELPSHADAELLLGTHDDPADGVLAAWESRIYRLGR"
     gene            complement(2566..3963)
                     /locus_tag="PKJ61_11415"
     CDS             complement(2566..3963)
                     /locus_tag="PKJ61_11415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HOC14470.1"
                     /translation="MARSEDATTPAVVTGDFDRRNKWTFGVGTVGRDMVYTLVSLYLV
                     FFLSDVLKPSREDFLWATSLILLARLFDAVADIVMGAIVDNTRTRWGHYKPWIAGGVV
                     ASAVFTVLLFADLGLTGTAFVVGFALIYLLWSLSWTANDIPYWSLLPALTLDQKQRER
                     FGSLAKIFATIGLFAVVVAVIPVTSALTTTLGATKAWTLFAVGVVVIMLLGQAVTLIG
                     VKEPPIVAEQERTSLRELAQVVFRNDQLLWVAIAMVLFMTGYLTTTTFGTYYFKYVYG
                     DESMYSPFGAVLVASQLLGYAIFPTLSSRFSRRTLYTAATALILAGYLVFFVSPTHIG
                     FIAAAGLLLFIGDSFITLLMLVFLTDTIEYGQWKLGRRNGAVTFALQPFINKVGAALS
                     TQIVAIAIVIAGVNPDRPADTTASGLLTIKLGMLVLPPILIVISYLIYRDKYRIDETF
                     YAQIVSDLRERGQLH"
     gene            4312..>5900
                     /locus_tag="PKJ61_11420"
     CDS             4312..>5900
                     /locus_tag="PKJ61_11420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14471.1"
                     /translation="MTTDKIQFAAGETFTLTAIVDQPLEDSASTLIIRDVTDSVDVKQ
                     CTSGTHCEAELGFASGGPHEYRAAVGAVESQAVTVSRSAWDIVLDVDRIEIGAGEHAS
                     LVATLNQDLSSTSGAYSVAIFDVTRARRAQTCVAGSACQVEIPAYTDDAISAQFIAAV
                     VSADDVVSQTVVEAADIQAVSEPVTIARKAWTLQVSVERDVLAAGERTTVHLRADQNV
                     RDTRGRYSLYIFEMSKNVLIAQCDSGTECSAGIDWVTVDPWAGHFMGVVAERRSEPPS
                     SLGDLQDLIGLGSEQPAVAQQGWEITVESDRQQLAPGDTAQIKATANQNLGLTSDRLT
                     LYIADMDGRTILAECNTGTTCDAQTAYYRRGDDGRHLILVEAIVAPSGAASFDELLRS
                     GWRGDSNRLRIDRTPWQSALVHTGGSTLTLIANQNVGLSFGSWAWYLFNAQTGSRVGK
                     CFTGKRCVFSGASRGGQYAALLAPATDPSSIDDVTYVLTASVFGSAPSPVFGPTLTSP
                     LSGRDETAGGQNLAEGPCACGR"
BASE COUNT         1067 a         1799 c         2001 g         1033 t
ORIGIN      
        1 cagaccggtc aatactggtc gggcgtgacc ggggtgagca tgggctcgct ggtgggtgcc
       61 gtggtcttcc tggtgccggg cgttttggcc ggtggcgcgt cactgtggca gccgcaggtg
      121 ctgctgctgg ggtcggtggt ggcggtgatg agttcggtga tcccatacgg gctggagatg
      181 gtggcgctgc gcagtctccc gtccggactg ttcgggattc tgatgagcct cgaacccgcg
      241 gcggcggcgc tcgcggcgct ggtggtgctc aaggagcaac tgagcatggt cgagattgtc
      301 gccatggctt gcgtgatcat cgcctcggtt ggtgcgacgc ggacgctggc caaggcgccc
      361 gcgccgaact gagtcgagat cagcgcggta tccaggccgc gagcgccgcg agcagagcga
      421 agagctgcat catcaggatc atcgtcggca cgatggccag gatcaccagc gagatccaga
      481 tccagatcat caccctggtt aaccgcgcga taaactgcag atcatcggag gtctgccggg
      541 tgagtccgta gccgtagtgc ggatcggtga aggcgtagcc cgggaccgga gtggacgggg
      601 cggctggacc cacggcccgc tgcggagggt gccaggtggt gccgtcgaag gtgctgcctg
      661 aactgtccat tcggggcacc caggcagtgc cgttccacac gtagccgatc gctcccctgg
      721 tggctcgatc gcaagctcgc tcccgaaagc gactcgcgct cccgaaagtt cgggagcaaa
      781 ggtcccattc gggagcgaag tcgaggggct ggtcctgagg actgttggcc cccttcccga
      841 tctcagctca tcgacccagg cggtagatcc gtgactccca agcggccagg acgccgtccg
      901 cggggtcgtc gtgggtgccg agcagcagct cggcgtccgc gtgggacggg agttcggacg
      961 gcaacgccac cggcgccgac gaccagttcg cgagcaccag caggcgctcc tcgccgaggg
     1021 tgcgggtgaa gcagaacagc tgctcatggt ctcccagcaa cagctggaag cgaccgtgtt
     1081 gaaccacctc cagctcatgg cgcaacccga tcaacgcccg gtagtggttg aagaccgaac
     1141 cggggtcacc cacctgcgcg gccgcattga tctcggcgtg attcgcggtc accggcagcc
     1201 acggcgtccc gtcagtgaac cccgcggtgt cggacgcgtc ccactgcatc ggggtgcgcg
     1261 cgttgtcgcg gctcttgaac gcgatcaccg ggagcagctg atttggatcg gccccggacg
     1321 ccacggcctg agcgtagaag ttcaccgact cgatgtcgtc gtagtgctcg atcgcactga
     1381 acccggcatt cgtcatgccc agctcctggc cctggtacac gtacggcgtg cccttgagca
     1441 gatgcagcgt ggtggccagg gtcttcgccg aagccacccg gaactccggc gagtcgtcac
     1501 cgaagcgcga caccgaacgc ggctggtcgt ggttctcgaa gtacagcgag ttccagccga
     1561 catcggccag cccgtcctgc cacttcgcca ggttcgcctt cagcgcgggc agttgcagcg
     1621 gcaccagctc ccacttcagg ccggaagggc ccgcgtccac gctcatgtgc tcgaaagtga
     1681 acaccatgcc cagttcgccg gcggccgggt cggtgtggga gcgggcggtc tcgacgtcca
     1741 ccaggaccat ctcaccgacg ctgaacagct ccttggccga gatgccgacc tcgcggttca
     1801 gttcgcgcag gaactcgtgc agccgcggcc cctccaccac caacgtcggg tcgtagcaga
     1861 tgccgtccgg cgcggccggg ccgtcggcca gcggcaaggt cttcgagatc aggttgatca
     1921 cgtccatgcg gaaaccgtcc acgccgcggt cgacccagcg ccgcatcatc gcgtagatct
     1981 ccgctcggac ggccgggttc tcccagttca ggtcgggctg cttgggtgcg aacaggtgca
     2041 ggtagtactc gccggacgcg gggtcgaact gccagccgcg gccggagaag aacgagcccc
     2101 agttggtcgg ctcagcgccg ggcgtgccgg gctcgaagcc tggccgcgcg ggtcgccaga
     2161 agtaccaatc ccgcttgtcg gacgcaggat cgcgggactc gacgaaccat gggtgctcgt
     2221 cggaggtgtg gttcacgacc aggtccatca ccagcttcat gccgcggtca tgcaggccgg
     2281 cgatcagcgc gtccaggtct tcgagcgtgc cgaacagcgg atcgacgtcg tcgtagtcgc
     2341 tgatgtcgta gccgttgtcg gccatcggcg agcggtagac cggcgacaac cagaccacgt
     2401 ccacgccgag cgcggccaga tggtcgaggt gggcgatgat gccggggagg tcgccaatcc
     2461 cgtccgcatt gctgtcggcg aacgaccggg gatagatctg atagaccacc gcgctgtgcc
     2521 accaaggagc catgcgcaca ttcttgcgga cgcgcgtgct gccggtcagt gcaactgacc
     2581 gcgttcgcgc aggtcgctga cgatctgggc gtagaaggtc tcgtcgatgc ggtacttgtc
     2641 gcggtagatc aggtagctga tcacgatcag gatcggcggt agcaccagca tgccgagctt
     2701 gatcgtcagc agtccggacg cggtggtatc ggccggacgg tcagggttga ccccggcgat
     2761 gacgatcgcg atcgccacga tctgggtgga gagcgccgcc cccaccttgt tgatgaacgg
     2821 ctgcagcgcg aaagtgaccg cgccgttgcg gcgtccgagc ttccactggc cgtactcgat
     2881 cgtgtcggtg aggaagacca gcatcagcaa ggtgatgaac gagtcaccga tgaacagcag
     2941 cagaccggcg gcggcgatga agccgatgtg ggtgggcgaa acgaagaaga cgagatagcc
     3001 ggccaggatc aacgcggtcg ccgcggtgta cagcgtccgc cgggagaagc ggctggacag
     3061 ggtgggaaag atcgcatagc cgagcagttg gctggccacc agcaccgcgc cgaacggtga
     3121 atacatgctt tcatcgccgt agacgtactt gaagtagtag gtgccgaagg tcgtggtggt
     3181 gaggtagccg gtcatgaaca ggaccatggc gatggcgacc cacagcagtt ggtcgttgcg
     3241 gaagaccacc tgcgccagtt cgcgcaggct ggtgcgttcc tgctcggcga cgatcggcgg
     3301 ttccttgacc ccgatcaggg tgaccgcctg gccgagcagc atgatcacca ccaccccgac
     3361 ggcgaacagc gtccatgcct tggttgcacc gagggtcgtg gtgagtgcgc tagtcactgg
     3421 aatcaccgct acgactacgg caaacagtcc gatggttgcg aagatcttgg cgagcgagcc
     3481 gaaccgctca cgctgcttct ggtcgagggt cagggcaggc agcagcgacc agtaggggat
     3541 gtcgttggcc gtccaggaca ggctccacag caggtagatc agggcgaaac cgacgacgaa
     3601 ggccgtcccg gtgaggccca ggtcagcgaa gagcagcact gtgaacactg cggacgcgac
     3661 cacgccgccc gcgatccagg gcttgtagtg gccccagcgg gtgcgggtgt tgtcgacgat
     3721 ggcgcccatc acgatgtcgg cgaccgcgtc gaacagccgg gcgagcagga tcagcgaggt
     3781 ggcccagagg aagtcctccc gtgacggttt gagcacgtcg ctgaggaaga agaccaggta
     3841 caggctgacc agggtgtaga ccatgtcgcg gccgaccgtg ccgacgccga acgtccactt
     3901 gttgcgccgg tcgaagtcgc cggtgacgac ggctggcgtt gtcgcgtcct cagagcgagc
     3961 catgggtctg agtcttgcgc ataaccgctt ccacggatgg gcatcccgga gccagatcgt
     4021 tcgtcatccc acccactcag cgaccaagtc atcccggcgc aggccgggag ctccgcaggg
     4081 caagggttcg atctcgaaag tgttcgaacc ttactggtcg ataaacgcat tccccgtcct
     4141 cggcgccggt cgtactaata ccttgcccgc atggaaatgc gtgctttcgg gtcgggtcgt
     4201 cgcctatgcg gattgttcac gtcttgggtg gtggcgtcca gcctggtctt cttcgcccca
     4261 tcgcttgtac cagaagccgt cgctgaccct ttgccgccgt cccaagtctt gttgaccacc
     4321 gacaagatcc agttcgctgc cggggagacg ttcactctca cagcgatcgt ggatcaaccc
     4381 ctggaagaca gcgcaagcac gctcattatc agagatgtca ccgacagtgt cgacgtcaag
     4441 caatgcacaa gcgggactca ctgtgaggct gaactcgggt ttgcgtcggg tggtccgcat
     4501 gagtacaggg cggctgtcgg agcggtggaa tcacaggccg tgacggtctc acggtcagca
     4561 tgggacatcg ttctggatgt tgaccgcatc gagatcggtg cgggcgagca cgcgagtttg
     4621 gtcgccacgc tgaaccaaga cctctctagc acctctggcg cgtattcggt tgcgatcttt
     4681 gatgtgacga gggcgaggcg agcccaaacc tgcgtagcgg gttcagcatg ccaagttgag
     4741 attccggcat acacagacga tgcgatttcg gcccaattca ttgcggccgt cgtttctgcc
     4801 gatgatgtcg tctctcagac agtcgttgag gcagcggata tccaagctgt gtccgagccg
     4861 gtcactatcg ccaggaaggc gtggacactt caagtatcgg ttgagcggga cgtgctggct
     4921 gcgggcgagc gcacgacggt gcatctgcgc gctgaccaga acgtaaggga cacacgcgga
     4981 cgctactcgc tgtacatctt cgagatgagc aagaacgtcc tgatcgctca gtgtgacagt
     5041 ggcacggagt gctcagcggg tatcgactgg gtgacggtgg atccatgggc cggccacttc
     5101 atgggagtgg tcgccgagcg cagatccgag ccaccttcat ctttgggtga tcttcaagac
     5161 ctcattggtc tcgggagtga gcagccagcg gtcgctcaac agggttggga gatcactgtc
     5221 gagtcggatc ggcagcaact cgcccctggg gacacggcgc agatcaaggc gacagcgaat
     5281 cagaatctcg gactgacgag cgaccggctg acgctctaca tcgccgacat ggatgggcga
     5341 acgatcttgg ccgaatgcaa cactggcacg acatgcgacg cccagactgc ctattaccgg
     5401 cgtggggatg acgggcgaca cctgatcctg gttgaggcaa ttgtcgctcc gtcgggggcg
     5461 gcctcgttcg acgaactgct tcgctccgga tggcgcgggg attccaaccg actgcgtatc
     5521 gaccggacgc catggcagag cgcgctcgtg cacacgggtg gtagcaccct caccctcatc
     5581 gccaaccaga atgtcggcct ctcattcgga tcctgggcct ggtatctgtt caacgctcag
     5641 accgggtcga gggtcgggaa gtgcttcacc ggcaagaggt gtgtcttcag cggggcatct
     5701 cggggtgggc aatacgccgc cttgctggct ccggccaccg acccgagctc gatcgacgac
     5761 gtaacctatg tgctcaccgc gagcgtgttt ggcagcgcac ccagcccggt gtttggtccc
     5821 acgctgactt cgccgttgag cggtcgggac gaaaccgcgg ggggccagaa cctggcggag
     5881 gggccgtgtg cctgcgggcg
//
LOCUS       DAOPRQ010000113         5843 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_11166_length_5843_cov_11.484105, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000113 DAOPRQ010000000
VERSION     DAOPRQ010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 5843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5843
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11166_length_5843_cov_11.484105"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1144)
                     /locus_tag="PKJ61_11425"
     CDS             complement(<1..1144)
                     /locus_tag="PKJ61_11425"
                     /inference="COORDINATES: protein motif:HMM:NF014741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HOC14472.1"
                     /translation="MEEQNGVAPVEVALAAIGAALNSIDPNRARLAPEARLRVAMTAR
                     SLTGRLDALASVLLAEADAARASERTAGTPTSTWMAIDQNLTKREAAGALHRAAELAA
                     HPVLGAAAVAGQVSSGQVRAINTVLGGLAPQLDAAQQDKAEQLLVGMAGHLDSDALSK
                     AAPQVLARVAPADANELLESKLQREAEAAQRSRSLRFWKQGGSVRFEGCLPRVEGEQF
                     ITLIDTNTEALRRTAVEARDPLFVNATPEQRRADALIHVLGHAATATPQPGVGAAKVI
                     VKLDYHQLKSGLAGAGLVGDGVQLSAGELRRACCDAELIPVVLRGGSEVLDVGRSRRL
                     VTPAMRAALIARDGGCVFPGCTAPPAACEAHHVVPWYLGGVTALSNL"
     gene            complement(1292..1666)
                     /locus_tag="PKJ61_11430"
     CDS             complement(1292..1666)
                     /locus_tag="PKJ61_11430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14473.1"
                     /translation="MIFFDESYELGSDVTLRRVGGHFRGQTVAAWRTGNRGRGVLLAG
                     DAVFPNPDHRTVIFLRSYPNRLPLSGAVVLRISAQLDGLHFDRLYNNFGAVVGSDAKS
                     VVRSSAVRHAAWTNGEHDAETW"
     gene            complement(1862..5020)
                     /locus_tag="PKJ61_11435"
     CDS             complement(1862..5020)
                     /locus_tag="PKJ61_11435"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016667007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HOC14474.1"
                     /translation="MALISEAEWESTALEVLAEQGWAPSTGAELAHERASASDLVLTE
                     RVHEAVRKLNPSVPALYLRQAVTEILTPASQDAMSENRRMHEFLVHGYRGVTYLDNDG
                     VEQNPTIRLVSVEPSDNDWVVAQQVRLDNAEHHRRFDVVGYLNGLPVIVAELKQAGLA
                     NATLASAHGQLGTYLAEFPLAFRCCVATLISDGVTAAYGTPFTPLHHYASWNVDDDGE
                     PLALGDSIVDTDYDTPLEVTLNGLGNQLRFLDLMRNFTAFDAGDFGLAKRVAKPHQYF
                     AVNKALGRTVTAVKSDGKAGVVWHTQGSGKSMEMELYANLVLRSTHLLNPTIVVITDR
                     RELDGQLFGAFDASTLLPEKPIQIRTRSQLREELSQRSSGGILFTTLQKFNRTLEERE
                     AGADHPLLSDRRNIVVVVDEAHRSHYDDLDGYARFLRDALPHATLIAFTGTPISEVER
                     NTREVFGDYIDIYDLTRAVTDGATVPVYFEPRLVKVSFAEEVTAEDLDRAADEATTGL
                     DEVERERLEKSVAVINAVYGAPARLATLAADIVAHWETRRARMRPLIDGPGKAMIVGA
                     TRQICADLYDQLIALRPEWHADSLEAGVVKVVYSGTASDPEPIRRHVRRESENQVVKK
                     RLKDPDDELEIVIVKDMMLTGYDSPPLHTLYLDRPMKGALLMQTLARVNRTFRGKDSG
                     LLVAYAPLVENLSAALAEYTLTDQATRPVGRNAAEGAELARALVDQLRTLVAGYDWRA
                     KLGGPKGWVKAAVGLTAWLRNPQNPGNQVAEGELGVAGRYRQLAGQLGRAWALASGAD
                     NLGDLRDEVRFYEEVRVWMAKYDAAERTARGEPVPEEVGRLLRQLAYEATGSDEVVDI
                     YSAAEIAPPNFNDLTPAVLAQAQRSANPHLAIEALRDAILAEAARSAGASLVRQRAFS
                     ERLTAVMLRYTNSQLTAADVLLELWELAQDISAEAKRGEQFTPPLGRDELATYDAIAD
                     NASALDVLGQDVLADIARQLVTLMRADVKTDWTVRDDVRASLRAKIRRLLRKYKYPPD
                     SAEGAVKQVIEQMELLAPGVAA"
     gene            5207..5440
                     /locus_tag="PKJ61_11440"
     CDS             5207..5440
                     /locus_tag="PKJ61_11440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14475.1"
                     /translation="MRFETIESDEFNLEHATRRASASEIEQAITNAEQMLRHRVSGRR
                     LIRARTNGGRRLAIVAELKDNGRRVRPITAWEE"
     gene            5443..>5843
                     /locus_tag="PKJ61_11445"
     CDS             5443..>5843
                     /locus_tag="PKJ61_11445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14476.1"
                     /translation="MMDTNGMSEAELADYYDRTHDISGFDLEHPEPITPARGRLDVTI
                     SVRFSSEEIDLLRAEAERAGMKVTAFIRALALQHASGQVLDRAALRGQLARVSQDLRA
                     LEHELGAWLADPEHAAEMAESAEAIRQGRAL"
BASE COUNT          968 a         2076 c         1855 g          944 t
ORIGIN      
        1 ccaggttcga cagcgcggtc acaccgccca aataccaggg caccacatga tgggcctcac
       61 aagccgcggg tggcgcggtg cagcccggga acacacagcc accatcgcgg gcgatcaggg
      121 cggcccgcat cgcgggggtc accagccggc gggaccggcc cacatccagc acctcgctgc
      181 caccgcgcag caccactggg atgagctcgg cgtcgcagca ggcacggcgt agctcaccag
      241 ccgacaactg aacaccgtca cccaccagcc cggcaccggc cagaccggat ttgagttggt
      301 ggtagtcgag tttcacgatc accttcgccg cgcccacccc cggctggggc gtggcggtcg
      361 cggcgtgacc cagcacgtgg atcagggcat cggctcggcg ctgttcgggg gtcgcgttca
      421 caaacagcgg gtcacgggct tcgacagcgg tgcggcgtag cgcttcggta ttggtgtcga
      481 tcagagtgat gaactgctca ccctccacac gtggcagaca gccctcgaac cgcaccgagc
      541 cgccctgctt ccagaatctc aacgaacgac tccgctgcgc cgcctccgct tcacgctgca
      601 acttcgactc aagaagttcg ttggcatcag cgggcgcgac gcgggccaac acctgcggcg
      661 cggccttgct caacgcatcc gaatccaggt ggccagccat gccgaccagt agctgttcgg
      721 ccttgtcctg ctgcgcggca tccaactgcg gggccaagcc gcccagcacg gtgttgatcg
      781 cccgtacctg accagaactc acctgcccag caaccgcggc cgcacccaac accggatggg
      841 cggccaactc ggcagcccga tgcaaagcac cagcggcctc acgcttggtc aggttctgat
      901 cgatcgccat ccacgtcgac gtcggcgtac ccgccgtccg ctccgacgcg cgcgcggcgt
      961 ccgcctcagc cagcaacacc gacgccagcg cgtccaaacg gccagtcagg ctccgagcgg
     1021 tcatggccac gcgcagacga gcttcggggg ccagccgggc acggttggga tcgatcgagt
     1081 tcagagcagc accgatcgcg gccagcgcaa cttccactgg cgccacaccg ttctgctctt
     1141 ccatgagaca agccaacaac ccgcctctga cacttctcgt ccaaaaccgc cccaaacggg
     1201 cgtaccgccc acactctcag gaaactttca ggaatggggg tggtagccct tagacggggc
     1261 gaggcatggt gacgtcgaat cggccctcca ctcaccaggt ttcagcgtcg tgctcgccat
     1321 tcgtccaggc agcgtggcgg actgccgaac tcctgaccac gctcttcgcg tccgacccga
     1381 ccacggcgcc gaagttgttg tagagccggt cgaagtgcag gccgtccaac tgcgcagaga
     1441 ttctgagcac caccgcgccc gacagcggca gccggttcgg atagctgcgc aggaagatca
     1501 ccgtccggtg gtccgggttc gggaagacgg cgtccccagc gagcagcact cctcggccac
     1561 gattgcccgt ccgccacgca gcgaccgtct gaccgcggaa atggccgccc acccggcgca
     1621 gcgtcacgtc cgatccgagt tcgtaggact cgtcgaagaa gatcacgcca tcagtgcggc
     1681 ggcaccactc atcgcgcaca cctcgggcgg cacttcgagg tcggcgtctc cacaccgcag
     1741 gtcacgcaaa gccagatcat ccgccgatgt tgggtcgctt gcgtctgctc gtcgagcatt
     1801 catccgcgga cggttaacac cacgtcttga cgatctgctg ccgccgtcca gcccgaacgg
     1861 cctacgccgc cacaccgggc gcgagcaact ccatctgctc gatcacctgc ttgacagctc
     1921 cttcggccga atccggcgga tacttgtact ttcgcagcag tcgtcggatc ttggcccgca
     1981 gagacgcccg gacgtcatcg cggacggtcc agtcggtctt cacgtccgcc cgcatcagag
     2041 tgaccaattg ccgggcgatg tcggcgagaa cgtcctgacc cagcacgtcg agggctgagg
     2101 cgttgtcggc gatcgcgtcg taagttgcca gctcgtcgcg tcccaagggc ggggtgaact
     2161 gctccccgcg cttggcttcg gcggagatgt cctgggcgag ttcccacaac tccagcagca
     2221 cgtcggcagc ggtcagctga ctgttggtgt agcgcagcat cactgcggtc agtcgctcgc
     2281 tgaacgcccg ctgccgcacc aggctcgctc cggccgagcg cgccgcttcg gccaggatcg
     2341 cgtcccgcag cgcctcgatc gccaggtgcg ggttggccga gcgctgtgcc tgggccagca
     2401 cggccggggt gaggtcgttg aagttcggcg gtgcgatctc ggcggccgag tagatgtcca
     2461 ccacctcgtc cgacccggtc gcttcgtagg cgagctggcg cagcagccgt ccgacttctt
     2521 cggggacggg ctcgccgcgg gcggtgcgtt cggccgcgtc gtacttggcc atccacaccc
     2581 gcacctcttc gtagaagcgc acctcgtcgc gcaggtcacc caggttgtcc gccccggacg
     2641 cgagcgccca cgcccgtccc aactgcccgg cgagctgccg gtagcgtccg gccacgccga
     2701 gctcgccctc ggccacctgg ttgcccgggt tctgcgggtt ccgcagccag gcggtcagcc
     2761 cgaccgcggc cttcacccag cccttcggcc cgcccagctt ggcccgccag tcgtagccgg
     2821 cgaccagcgt ccggagttga tcgaccagcg cccgcgccaa ctcggcaccc tcggcggcgt
     2881 tccgtccgac cgggcgcgtc gcctggtcgg tcagggtgta ctcggccagc gccgcgctca
     2941 ggttctccac cagcggcgcg taggcgacca gcaggcccga atccttgccc cggaaggtcc
     3001 ggttcacccg ggccagggtc tgcatcagca gcgcgccctt catcggacgg tcgaggtaca
     3061 gggtgtgtag cggcggcgag tcgtagccgg tgagcatcat gtccttgacg atcactatct
     3121 ccagctcgtc gtcgggatct ttgagccgct tcttcaccac ctggttctcc gactcccgcc
     3181 ggacgtgccg ccggatcggc tccgggtcgg acgccgtccc ggagtacacc accttcacca
     3241 ccccggcctc aaggctgtcg gcgtgccact cgggccgcag cgcgatcagc tggtcgtaca
     3301 ggtcggcaca gatctgccgg gtcgcaccga cgatcatcgc cttgcccggc ccgtcgatca
     3361 gcggacgcat ccgcgcccgc cgggtctccc agtgcgccac gatgtcggca gccagggtgg
     3421 ccagccgggc cggcgcccca tagacggcgt tgatcaccgc gaccgacttc tccagccgct
     3481 cccgctcgac ctcgtccagc ccggtggtgg cctcgtcggc ggcccgatcg agatcttcgg
     3541 ccgtgacctc ctcggcgaag gagaccttca ccagccgcgg ctcgaagtac accggcacgg
     3601 tcgcgccgtc ggtgaccgcc ctggtcaggt cgtagatgtc gatgtagtcg ccgaacacct
     3661 cccgggtgtt gcgctccacc tcgctgatcg gcgtcccggt gaacgcgatc agggtggcgt
     3721 gcggcagggc gtcgcgcagg aagcgggcgt agccgtccag gtcgtcgtag tgggagcggt
     3781 gagcctcgtc gaccaccacc acgatgttgc gccgatccga cagcagcggg tggtcggcgc
     3841 ccgcctcgcg ctcctccagc gtccgattga acttctgcag ggtggtgaac aggattccgc
     3901 cggacgaccg ctggctcagt tcctcgcgca gttgcgatcg cgtccgaatc tggatcggct
     3961 tctccggcag cagcgtggac gcgtcgaagg cgccgaacag ctggccgtcc aactcgcgtc
     4021 ggtcggtgat caccacgatc gtcggattca gcaggtgggt cgagcgcaac accaggttgg
     4081 cgtacagctc catctccatc gacttgcccg agccctgggt gtgccagacc acgccggcct
     4141 tgccgtcgct cttcaccgcg gtcaccgtgc ggcccagcgc cttgttcacc gcgaagtact
     4201 ggtgcggttt cgcgacccgc ttggccagcc cgaagtcgcc ggcatcgaag gcagtgaaat
     4261 tccgcatcag gtcaagaaag cgcagctggt tgcccagccc gttgagggtc acttccagcg
     4321 gagtgtcgta gtcggtgtcg acgatgctgt cgcccagggc cagcggctcg ccgtcgtcgt
     4381 cgacgttcca ggacgcgtag tggtgcagcg gcgtgaacgg cgtcccgtag gccgcggtca
     4441 ccccgtcgct gatcagggtc gccacacagc agcggaaggc cagcgggaat tcggccaggt
     4501 aggtgcccaa ctgaccgtgc gcggacgcga gcgtcgcgtt cgccagcccg gcctgcttca
     4561 actcggccac gatcaccggc agtccgttca ggtagccgac cacgtcgaag cggcgatggt
     4621 gttcggcgtt gtcgagccgc acctgctggg ccaccaccca gtcattgtcg gacggctcga
     4681 cgctcaccaa ccggatcgtc ggattctgct ccaccccgtc gttgtcgaga taggtgaccc
     4741 cgcgatagcc gtggaccaga aactcatgca ttcgccggtt ctcgctcatc gcatcctgcg
     4801 acgccggggt gaggatctcg gtgaccgcct gccgcaggta caacgccggc acggacggat
     4861 tcagcttccg cacggcctcg tgcacccgct cggtcagcac cagatcggac gcggacgcgc
     4921 gctcgtgcgc caactcagcg cccgttgagg gcgcccagcc ctgctcggcc agcacctcca
     4981 gcgccgtcga ctcccactcg gcctcactga tcagcgccat ggaactcttc cctcacattc
     5041 gtcgtcggtt gagctgtcga aaccggcccc aagccggctg tcagatgcga gtggcagcct
     5101 gcagcacgaa gggtggcgca gcgaccaatc cggggcggac ggccagcgcc ttggaatcgt
     5161 gttgcgcaca ggatagaatc gcaacacatc gcaggaggag ttcgcggtgc ggttcgagac
     5221 aattgaatca gacgagttca acctggaaca cgcaacgcgg cgcgcctccg cgagcgagat
     5281 cgaacaggcc atcaccaacg ccgagcagat gctgcgccat cgtgtcagcg gccgacggct
     5341 cattcgagct cgaaccaacg gtggtcgtcg cttggcgatc gtcgccgaac tcaaggacaa
     5401 cggacgccga gtccggccga tcacagcgtg ggaggagtga agatgatgga caccaatggc
     5461 atgagtgaag ccgaactggc cgactactac gaccggacgc acgacatctc gggcttcgat
     5521 ctggagcatc ctgagccgat cactcctgca cgcggccggc tcgacgtgac catctcggtg
     5581 cgattcagct ctgaggagat cgatctgctt cgagccgagg ctgagcgcgc cggaatgaag
     5641 gtgaccgcct tcatcagagc gctggctctg cagcacgcct ccggtcaggt tctcgatcgc
     5701 gccgcgcttc gcggccaact tgcccgagtc tctcaggatc tgagggcgct cgaacacgag
     5761 ttgggggcgt ggcttgccga tcccgagcac gccgcggaga tggcggagtc tgccgaggcc
     5821 atcaggcagg gacgcgcgct gag
//
LOCUS       DAOPRQ010000114         5840 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_11177_length_5840_cov_11.206223, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000114 DAOPRQ010000000
VERSION     DAOPRQ010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 5840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5840
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11177_length_5840_cov_11.206223"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="PKJ61_11450"
     CDS             complement(<1..437)
                     /locus_tag="PKJ61_11450"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017934702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOC14477.1"
                     /translation="MAQLTVGSPAPAFTLPDADGNPVSLADYPRRRVIVYFYPAALTP
                     GCTTQAVDFDAALAELVAAGYQVVGISPDQVAKLATFRDRNGLDFPLLADPDLEILKA
                     YSAYGTKMLYGKAIEGVIRSTFVIDVDEAGHGVVAVAQYNVKAT"
     gene            700..2085
                     /locus_tag="PKJ61_11455"
     CDS             700..2085
                     /locus_tag="PKJ61_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOC14478.1"
                     /translation="MRVLAAAASAVALLAMAACSSGTTATSAAPGATGTTSATADEKC
                     AAYAEYGDLTGKTISVFTSIAADAEAKPHTDSYKPFEECTGATIKYEGSREFESQLPV
                     RLQAGNAPDIAYIPQPGLLQTLVADNPGKVFEAGALATKNVETYYDPAWKGYGSVDGK
                     FYAVPVGANVKSFVWYSPKAFADKGYAVPQTWDELIALSDKIVADNPDGDVKPWCAGI
                     ESGTATGWPATDWVEDVMLRVNGPEVYDQWVNHEIAFNDPKVVAALDQVGTILRNDKY
                     VNGGLGDVKSIATTAFADAGLPVADGTCYMHRQASFYQANWDKGTVVGEDGDVWAFYL
                     PSMTADSKPVLGGGEFAAAFSDRPEVQAFQAYLASPEWSNAKAIATPNGGWLSANKKL
                     DINNLVKPVDKLSFSMLADEKAVFRFDGSDLMPSAVGAGAFWKQMTNWIALGKTSQEA
                     ADAIEKAWPAK"
     gene            2169..3146
                     /locus_tag="PKJ61_11460"
     CDS             2169..3146
                     /locus_tag="PKJ61_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020575114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOC14479.1"
                     /translation="MEWFLKPETLPEKIGVMVLAILLFAAVMGLVLAVVDRPRLPKWV
                     PAVGYLGPAVLLITFGLLWPGLNTIKNSFFDRLSQNFVGIANYQTIFTDMGFLIVLRN
                     TLLWVLLVPTVATALGLVYAVLVDRTRVEKFAKTLIFLPMAISMVGASIIWKFMYDMK
                     PGKTQIGLLNQIVVWLGGEPQPWLLNPPLNTFMMIVVMVWIQAGFAMTVLSAAIKAIP
                     DEIIEAAKVDGVTGIKLFFYVTIPSVRPAIIVVLTTIAMGTLKSFDIVYTMTGGNFES
                     SIVAQEFYVQSFVQGRPDIGAALAVLLFIMITPIIVYNVRQMRISEGER"
     gene            3143..4039
                     /locus_tag="PKJ61_11465"
     CDS             3143..4039
                     /locus_tag="PKJ61_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020575113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOC14480.1"
                     /translation="MSLDTHVVEQRKLTRKEQRDVDSKTRLASPGASLIAVLIAVFWT
                     IPTAGLLITSFRPQADIRRTGWWTALLNPKFTLDNYIDTLGANNLANSFFNSFIITIP
                     AVVIPILLALLAAYAFAWIDFKGRNFLFAFVFALQVVPIQVTLLPLLTQYVQWGLAGT
                     LWTVWLSHTIFGLPLAIFLLHNFMKEIPRSLIEAARVDGAGHVRVFFQVLLPLLLPAI
                     ASFGIFQFLWVWNDLLVSLTFAGPEYSPITRVIGELSGSFGSKWHLLSSGAFISMLVP
                     MIVFLSLQRYFVRGLLAGGVKG"
     gene            4255..4545
                     /locus_tag="PKJ61_11470"
     CDS             4255..4545
                     /locus_tag="PKJ61_11470"
                     /inference="COORDINATES: protein motif:HMM:NF023697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3618 domain-containing protein"
                     /protein_id="HOC14481.1"
                     /translation="MSETTRSKAEIEAEIAAARERLAASVEGLFMQVHPKAVVANTVS
                     EARAFVANGAQTVKAELVNVDGSLRIERVGLIAAAVAGSLAFLATVRSILRR"
     gene            4680..>5840
                     /locus_tag="PKJ61_11475"
     CDS             4680..>5840
                     /locus_tag="PKJ61_11475"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOC14482.1"
                     /translation="MDPWSEHGPITLAAGDSAIWPELVTGWNSLHPGEPVTLVNLPVS
                     SNRRLAAITERAQANSGEYTVIALEPPWTGELAAKGWLTELPAGRFAADGMAPAAFAS
                     ASWQKTLYAYPVTTDAGLLYYRSDLLADAGVAAPVTRAALQASCRQVGADSARSCFGL
                     SLAKDEPLTVAFTEAVVGAGGQVLTSAGEPGVESSAAVSGLDRLAEDLDSRIIPEAAL
                     SWSEADAVREFAKGNLVFLRGWATAGAVLDSATPGTVVVGKTGLALLPGQTEPAASVL
                     GGENLAISAFARNRGTAADFLAYAASDVTQRRLVEKGSIGPVLSGLYGDADLVARHPY
                     LSVLDAALKAATVRPATQRYSEVTLAIQDQANGALRGEKTADQALTELQGRLE"
BASE COUNT          992 a         1811 c         1942 g         1095 t
ORIGIN      
        1 gtggccttga cgttgtactg agccaccgcg accaccccgt gaccggcctc gtcgacgtcg
       61 atcacgaagg tggagcggat cacgccctcg atcgccttgc cgtagagcat cttcgtgcca
      121 taggccgagt aggccttgag tatctcaagg tcgggatctg ccagcaacgg gaagtcgagg
      181 ccattgcggt cgcggaaggt ggccagcttc gcaacctggt caggagaaat acccacaacc
      241 tggtagccgg ccgcgaccaa ctcggccaga gccgcgtcga agtcgaccgc ctgggtggtg
      301 cagccagggg tcagcgcggc cgggtagaag tagacgatga cgcgtctgcg ggggtagtcg
      361 gccagtgaga cggggttgcc gtccgcgtcc ggaagggtga aagcgggagc agggctgccg
      421 acggtgagtt gcgccatggc ccaagattag ggcaccggcg gaggtgggct tcgatgggca
      481 accgggtcga cacgccggga aagctgccgg aaaggccacc gaaccggcca gaattccatc
      541 tggctttgac taggctgaat agtctgctaa atggttacag cttgataaca cttcgacaac
      601 gggggcccgt gagcgtagtc gaagggatag agtccgaaca cccctggttc ttaggggcga
      661 cctgacacag agaagtgagg actcatgtca caaccacgga tgcgcgtact agctgccgct
      721 gcctccgccg tcgcactgtt ggctatggct gcctgtagtt ccggcaccac ggccacctcg
      781 gctgctcctg gtgccaccgg caccacttcg gccaccgccg acgagaagtg cgcggcctat
      841 gccgagtacg gcgatctgac cggcaagacg atctcggtat tcacctcgat cgccgcggac
      901 gccgaggcca agccgcatac ggactcctac aagccgttcg aggaatgcac cggcgcgacg
      961 atcaagtacg agggctcccg cgagttcgag tcgcagttgc ctgtccgcct gcaggccggc
     1021 aacgcgcccg acatcgccta catcccgcag ccgggcctgc tgcagaccct agttgccgac
     1081 aacccgggca aggtcttcga ggcgggcgca ctggccacca agaacgtcga gacctactac
     1141 gacccggcct ggaagggcta cggctcggtg gacggcaagt tctacgcggt gccggtgggc
     1201 gccaacgtga agtcgttcgt ctggtactcg cccaaggcgt tcgcggacaa gggctacgcc
     1261 gttccgcaga cctgggacga gctgatcgcg ctctccgaca agatcgtcgc cgacaatccc
     1321 gatggcgatg tcaagccgtg gtgtgcgggc atcgagtccg gcaccgccac cggctggccg
     1381 gccaccgact gggttgagga cgtcatgctg cgcgtcaatg gcccggaagt ctacgaccag
     1441 tgggtaaacc acgagatcgc cttcaacgac cccaaggttg ttgcggcgct cgaccaggtc
     1501 ggcaccatcc tgcgtaacga caagtacgta aatggtgggc tgggcgacgt gaagtccatc
     1561 gccactactg ctttcgctga cgccggcctg cccgttgccg acggcacctg ctacatgcac
     1621 cgccaggcgt ccttctacca ggccaactgg gataagggca ccgttgtggg cgaggacggc
     1681 gatgtctggg ccttctactt gccgagcatg accgctgact ccaagccggt gctgggcggt
     1741 ggcgagttcg ccgcggcctt ctccgatcgt cccgaggtgc aggcgttcca ggcgtacctg
     1801 gccagccccg agtggagcaa cgctaaggcc atcgccaccc cgaacggtgg ctggctctcg
     1861 gccaacaaga agctggacat caacaacctg gtcaagccgg tcgacaagct gtccttcagc
     1921 atgctggccg acgagaaggc cgtgttccgc ttcgatggat ccgacttgat gcccagcgca
     1981 gttggcgctg gcgccttctg gaagcagatg accaactgga ttgccctcgg caagactagc
     2041 caggaagctg ccgacgcgat cgagaaggcc tggccggcta agtagcccgg gcacaaggca
     2101 atccacgttg ggtccgggta cgcttcggcg gcgcccggac ccaacgtgct ctctgagagg
     2161 actcaacaat ggagtggttc cttaagcccg agactctccc cgagaagatc ggggtgatgg
     2221 ttctggccat cctgcttttc gcggctgtga tgggcttggt gctggccgtg gtcgatcggc
     2281 caaggctgcc aaaatgggtg ccggccgtcg gttaccttgg tccggccgtc ctgctgatca
     2341 cctttggcct gttgtggccg ggtttgaaca cgatcaagaa ctcgttcttc gatcggctca
     2401 gtcagaactt cgtcggcatc gccaactacc agacgatctt caccgacatg ggcttcctga
     2461 tcgtgctgcg caacacgttg ctgtgggtgc tgctggtgcc caccgtcgcg acggccctcg
     2521 gtctggtcta tgcggtcttg gttgaccgga cccgggtgga gaagtttgcg aagaccctga
     2581 tcttcttgcc gatggcgatt tcgatggtgg gtgcctcgat catctggaag ttcatgtacg
     2641 acatgaagcc cggtaaaacc cagatcggcc tgctcaacca gatcgtggtc tggcttggtg
     2701 gcgagccgca accgtggcta ctgaatccgc cgctgaacac cttcatgatg atcgtcgtga
     2761 tggtctggat ccaggccggc ttcgccatga ccgtgctatc ggctgcgatc aaggcgattc
     2821 cggatgagat catcgaggcc gctaaggtcg acggcgtcac cggaatcaag ctgttcttct
     2881 acgtgactat tccgagtgtg cgtccagcaa tcatcgtggt gttgaccacg atcgccatgg
     2941 gcacgttgaa gtccttcgac atcgtctaca cgatgaccgg cggcaacttc gaaagctcga
     3001 tcgttgcgca ggagttctac gtgcagagct tcgtgcaggg acggcctgat atcggcgccg
     3061 cgctggcggt gctgctgttc atcatgatca cgccgatcat cgtctacaac gttcggcaga
     3121 tgcggatttc ggagggggag cgatgagcct cgatacccat gtggttgagc agcgcaagct
     3181 cacccgaaag gaacagcgtg acgtcgattc caagacccgg ctagcctccc cgggagcgtc
     3241 gctgatcgca gttttgatcg cggtcttctg gacgatcccg accgcaggcc tgctgatcac
     3301 gtccttccgt ccgcaggcgg atattcggcg gacggggtgg tggacggcac tgttgaaccc
     3361 gaagttcacc ctggacaact acatcgacac cctcggtgcg aacaacttgg cgaactcgtt
     3421 cttcaactcg ttcatcatca cgattccggc cgtggtcatc ccgatcctgc tggctctgtt
     3481 ggcggcgtac gcgttcgcgt ggatcgactt caagggacgc aacttcctgt tcgcgttcgt
     3541 gttcgcactg caggtggtgc cgattcaggt gaccttgctg ccgctgctca cgcagtacgt
     3601 ccagtggggt ctggccggca cgctgtggac ggtgtggcta tcgcacacca ttttcgggct
     3661 tcctttggcc atcttcctgc tgcacaactt catgaaggag attccgcgct ccttgattga
     3721 ggctgcccgg gtggacggtg ccggtcacgt gcgggtgttc ttccaggtgc tgctgccgct
     3781 gttgctgcca gcgatcgcgt cgttcggcat cttccagttc ctgtgggtct ggaacgacct
     3841 gctggtgtcg ctgaccttcg caggtccgga atactccccg atcacccggg tgatcggcga
     3901 actctccggc agcttcggca gcaagtggca cctactttcc agcggtgcct tcatctcgat
     3961 gctggtgccg atgatcgtgt tcctctcgct gcaacggtac ttcgtccgcg gtctgctggc
     4021 cggtggcgtg aagggctgag ttgttcgtag ttagccgagg gcctctccca ggtgggggag
     4081 gccctcggtg cgtccgcgcc tggtggaaag atggctgagc ccccattcca ccgtcatccc
     4141 ggcgcaggcc gggatctctc cccgccatcg tggatccagg ctgcttgacg aagcctgctg
     4201 cgcccatcgc aggtactggc ataggctgag cggcaggatc ctgcaggagg aacgatgagc
     4261 gagaccacca ggtcgaaggc cgaaattgaa gccgagatcg ccgccgctcg tgagcggttg
     4321 gccgcgagtg tggagggcct cttcatgcag gtgcacccca aggccgtggt ggcgaacacc
     4381 gtcagcgaag cccgggcgtt cgtcgcgaac ggcgcgcaga ccgtcaaagc cgaattggtg
     4441 aatgtggacg ggagcctgcg catcgagcgt gtcggtctga tcgcagccgc cgtggccgga
     4501 tcgcttgcgt tcctggccac cgtccgctcg atcctacgga ggtagtgctg gtacccggac
     4561 gcgtccgcag ggggttcggt tggtcgagct ggtcgagacc cctcgcggcc gcattgacac
     4621 tggttaccct cgctgcttgc acaccggcca gcccgccgcc cgccccgacg ccgacgatcg
     4681 tggacccgtg gagcgagcat ggcccgatca ccctcgcggc cggcgactcc gcgatctggc
     4741 ccgagctggt gaccggctgg aactcactgc acccggggga gccggtgacg ttggtgaacc
     4801 tgccggtgtc gtcgaatcgg cgtctggcgg ccatcaccga gcgggcgcag gcgaatagcg
     4861 gggagtacac cgtgatcgca ctggagccgc cgtggaccgg cgaacttgcc gccaagggct
     4921 ggctgaccga gttgccggcg ggccgattcg ccgctgacgg gatggccccg gccgcgtttg
     4981 cgtccgcttc gtggcagaag acgctgtacg cgtacccggt gaccactgat gccggactgc
     5041 tctactaccg cagcgacctg ctggcggacg cgggggtggc cgcgccggtc acacgggcgg
     5101 cgctccaggc gtcctgccgg caggtcgggg cagactcggc tcggtcatgc ttcgggctgt
     5161 cgctggcgaa ggatgagcca ctgacggttg ccttcaccga agccgtggtc ggggctggtg
     5221 gccaggtgct gacctctgcc ggcgaacccg gggtggagag ttcggctgcg gtcagtggac
     5281 tggaccggct cgccgaagat ctcgattctc gaatcatccc ggaggccgcg ttgtcctgga
     5341 gtgaagcgga cgcggtccgt gagttcgcca agggcaacct ggtgttcctg agaggctggg
     5401 cgacggccgg tgcggtgctg gattcggcca cgccgggcac cgtggtggtg ggcaagaccg
     5461 ggctggcgct actgcctgga cagactgagc cggccgcgtc cgtccttggc ggggagaacc
     5521 tcgcgatctc tgcgttcgcc cggaaccgcg ggacagccgc cgacttcctg gcctacgcgg
     5581 cgtcggacgt cacccagcgc cgcctggtgg agaagggttc gatcggtccg gtgctgagcg
     5641 ggctctatgg cgacgcggac ctggtggccc ggcatccgta tctatccgtg ctggacgctg
     5701 ctctgaaggc cgctacggtg cggccggcga cgcagcggta cagcgaagtg accctggcga
     5761 tccaggatca ggccaacggg gcgctacggg gcgagaagac cgccgaccag gcgctcaccg
     5821 agctgcaggg aaggcttgag
//
LOCUS       DAOPRQ010000115         5391 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_12365_length_5391_cov_13.542166, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000115 DAOPRQ010000000
VERSION     DAOPRQ010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 5391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5391
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12365_length_5391_cov_13.542166"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..914
                     /locus_tag="PKJ61_11480"
     CDS             <1..914
                     /locus_tag="PKJ61_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HOC14483.1"
                     /translation="DTGEATGLGPYGLTITFGFGRSLFTTDDGRDRFGLDGLLPEQFT
                     PLPKMTNDFIDPARSGGDLCIQACANDPQVAVHAIRNLTRIGFGRTELKWSQLGFGRT
                     SSTSTAQTTPRNLFGQKDGTNNLKAEEPERLRDNVWITDGPEWSLGGSYLIARRIAMT
                     IEVWDGVRLEEQNRVLGRDKATGAPLSGGEEFTAPDFAASADGEPLIDPRSHVARTSP
                     ENNGGIAMLRRGYNYVDGNDEVGRLNAGLFFIAFISSPERFAKVHTNMARDDMFVEYL
                     KTTSSSVFLVPPGIKEGQYVGQPLFEA"
     gene            complement(1067..1363)
                     /locus_tag="PKJ61_11485"
     CDS             complement(1067..1363)
                     /locus_tag="PKJ61_11485"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HOC14484.1"
                     /translation="MLIVHVFVRVVPEGIDEFIAATRANAEASRHEPGIARFDVVQSL
                     DDPASFVLVEAYRTDQAALDHKQTEHYAVWRDAVAPLMAEPRTSLKHRDVYYPD"
     gene            1545..2795
                     /locus_tag="PKJ61_11490"
     CDS             1545..2795
                     /locus_tag="PKJ61_11490"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOC14485.1"
                     /translation="MPTSQTRELVANQPYLVLLAARTISMLGMAFSPVALAFGVLRLP
                     GGDAGSLSIVLAAQTLPMVVFMLVGGVVADRYPRSVVLLTGEWLAALAWGAIGVMMLT
                     GYAPIWLLSVAAALAGIAGSLIYPALNGIIPDLVPEHVRQRGNAWLAMGASAARLAGL
                     VAGGAVVVWLGGGWAMIVACAMYAIAGVLIMGLPKVTGTLAGAQEHPIRQLIDGWGEF
                     SSRQWLWVVVLQWSVMLMMLQAAHGVLGPVVAEAELGGAAAWTAILAGEALGAVVGVA
                     IAMFWKPRRPILVATLLTLSAGVPAVLLGFAFPLWTVILAAFVMGIGFDLFGVLWMTT
                     MQDEVPPESLSRVASYDALGSLMLGPLGLVLAGPATLLFGAHNALIGSGVIAIVTAVF
                     ALAFPEVRQLEAKRPAAAEVVEAA"
     gene            2921..3838
                     /locus_tag="PKJ61_11495"
     CDS             2921..3838
                     /locus_tag="PKJ61_11495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14486.1"
                     /translation="MASLTPALTAWVRELAADQDARITATTPVVPLVTRGLPTRPLAP
                     EHSQVYVCAGVIVKLHGTGTDAQRLTRRLAGLAAPDMDRLWIQPLRYRPIPAPDGRVA
                     SIWPRVSVLSTVDRPPWPEVGTLLARLHRMPIGGDLPRLGGHSRLARAVVFLRTLGAE
                     WAWLVNLGEHLADALARPGPNTLAHGDFHLGQLGHTPLRRSWKLLDVDDFGLGDPAWD
                     LARPAGFWAAGLLADEAWATFLAAYREAGGPAVPADGDPWPALDLPARAAVLVATVQA
                     LRLSHGEPTVEPLLAACRRMQTGTHDPSE"
     gene            3822..4130
                     /locus_tag="PKJ61_11500"
     CDS             3822..4130
                     /locus_tag="PKJ61_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HOC14487.1"
                     /translation="MTHPSDPPRYEPYAGPQAAAGAMLCPKCGGPMRTYERNGIHLEQ
                     CSGCRGVFLDFGELEHLTQLESRFAQQPPPQPNYGPAWGSYGNQKYRRGGLSGLFFSS
                     "
     gene            complement(4295..>5391)
                     /locus_tag="PKJ61_11505"
     CDS             complement(4295..>5391)
                     /locus_tag="PKJ61_11505"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HOC14488.1"
                     /translation="VAGLIWARRESRDEVFAGVTPGLLPAPGQPSERRLLSRGEQPPI
                     AVRFEPPTGLRPGLAGVIIDSRVDAVELSATLIDLAIRGWLVLKPIAPDGSQTRPNGW
                     ELHQVPNPPAEALTRTEQAVLAAAFGNGPVSTVAELRAGHRLQEAARLLGEESAEHGW
                     FRPASARWVRSVGWIALLLGTFLISTGLIWAGLGALAAGVIAVAGTRGLGQPLSAEGY
                     AARVQTLGFRQYLATAEAEQLRFEVGVDVFGRYLPYAMVLGVVDHWRTVFANALRADL
                     AAGEELAGFGWLALDDALTGLLLVDLLTSDFGLLDNLGEGFGGELEGVDAGLDSSDIG
                     ADSGGIDWGGGGDGGDGGWGGFDGFDGGFD"
BASE COUNT          833 a         1865 c         1819 g          874 t
ORIGIN      
        1 acgacaccgg tgaggccacc gggctcggcc cgtacggctt gaccatcacg ttcggcttcg
       61 gccggagcct gttcaccacc gacgacggca gggaccgctt cggcctggac ggcctattgc
      121 ccgagcagtt cacaccgctg ccgaagatga ccaacgactt catcgatccg gcgcgctccg
      181 gtggcgacct ttgcatccag gcgtgcgcga acgacccgca ggtggccgtg cacgcgatcc
      241 gcaacctgac ccggatcggc ttcggtcgca ccgagctgaa gtggtcgcag cttgggttcg
      301 gacgcacgtc gtccacttcg accgcgcaaa ccacaccgcg caacctgttc ggccagaagg
      361 acggcaccaa caacctgaag gcagaggagc ctgagcggct gcgcgacaac gtgtggatca
      421 ccgacggccc cgaatggtcg ctgggtggtt cgtacctgat cgcgcgccgg atcgcgatga
      481 ccatcgaggt gtgggacggc gtccggctgg aggaacagaa ccgggtgctc ggccgggaca
      541 aggccaccgg cgcaccgctg tcgggcggcg aggaattcac cgcgcccgac ttcgccgcgt
      601 ccgctgacgg tgagccgctg atcgatccgc gttcacacgt ggcccgcacc agccccgaga
      661 acaacggcgg catcgccatg ctgcgccgcg gctacaacta cgtcgacggc aacgacgagg
      721 tcggccgcct gaacgccggc ctgttcttca tcgcgttcat ttcctcgccc gagcgcttcg
      781 cgaaggtgca caccaacatg gcccgcgacg acatgttcgt cgaatacctg aagaccacct
      841 cgtcctcggt cttcctggtt ccccccggca tcaaagaagg ccaatacgtc ggccagcccc
      901 tgttcgaggc ctgaccgagg gcggcgtccg agccccgaac ttccgattgt tctcggagat
      961 cccgcctgcg ccgggatgac gcggaccagc gaagcctgaa cgagaagggg acggttcctt
     1021 ttccgttcaa gttatccacc ggcggacctc cggccggtcc gtccactcaa tcggggtagt
     1081 agacgtcccg gtgtttcagc gaagtcctcg gctcggccat cagcggcgcg accgcatccc
     1141 gccagacggc gtagtgctcg gtctgcttgt ggtcgagggc agcctgatcg gtgcggtagg
     1201 cctccacgag cacgaaggac gcggggtcgt cgaggctctg cacgacatcg aaacgggcga
     1261 ttcccggctc gtgcctggac gcttccgcgt tggcccgggt ggctgcgatg aactcgtcga
     1321 tcccctccgg gaccacacgc acgaagacat ggacgatcaa catggctcaa tcttcgcgcc
     1381 gacctggcag gtgccgatac aaaatgcccc gtcgattgcg aaaccgcagt gctgagcaga
     1441 tatttcgcga tcgacagggc agtttgtgac accaggcgtg ggttgcgtcc ggcgggcagc
     1501 ggtgagagca ttcaaggctg cccagaaccg aggaagaaac tcccatgccc acgagccaga
     1561 cccgcgaact cgtcgcgaac cagccctacc tggtgctgct ggctgcccga accatctcga
     1621 tgctgggcat ggcgttctcg ccggtggcgc tggcgttcgg cgtgctgcga cttcccggtg
     1681 gtgacgccgg ttcgctctcc attgtgctgg ccgcgcagac tctcccgatg gtggtgttca
     1741 tgcttgtcgg cggtgtggtc gccgaccgct acccccgctc ggtggtgctg ctgacgggcg
     1801 aatggctggc cgcgctggcc tggggcgcga tcggcgtgat gatgctcacc ggctacgccc
     1861 cgatctggct gctgagcgtt gccgcagcac tcgccggcat cgccggttcg ctgatctatc
     1921 cggcgctgaa cggcatcatc cccgacctcg tgcccgagca cgtccgccaa cgcggcaacg
     1981 cctggctggc gatgggtgcg tccgcggctc ggctcgccgg actcgtcgcc ggcggcgcgg
     2041 tggtggtttg gctcggcggt ggctgggcca tgatcgtggc gtgcgcgatg tacgcgatcg
     2101 cgggggtgct gatcatggga ctgccaaagg tcaccggcac gctcgccggc gcccaggagc
     2161 atccgatccg ccaactcatc gacggctggg gtgaattctc gtcccggcag tggctgtggg
     2221 tggtggtgtt gcagtggtcg gtgatgctga tgatgctgca ggctgcgcac ggcgtcctcg
     2281 gcccggtggt agccgaagcc gaactgggcg gcgctgcggc gtggacggcg atcctggcgg
     2341 gcgaagcgct cggggccgtg gtgggggtgg caatagccat gttctggaag ccgcggcgac
     2401 cgatcctggt ggcgacactg ctcaccttga gcgccggcgt gcccgccgtg ctgctcggct
     2461 tcgccttccc gctgtggacg gtgatcctgg ccgccttcgt gatgggcatc ggcttcgacc
     2521 tgttcggcgt gctgtggatg acgaccatgc aggacgaggt gccgcccgag tcgctgtctc
     2581 gggtcgcgtc ctacgacgcg ctgggctcgc tgatgctcgg cccgctcggt ctcgtgctcg
     2641 ccggaccggc caccctgctc ttcggcgccc acaacgcgtt gatcggttcg ggcgtgatcg
     2701 ccatcgtcac cgcggtgttc gcgctcgcgt tcccagaggt gcgccagctt gaggccaagc
     2761 gtcccgcggc tgcggaggta gtcgaggcgg cgtagccaat cggctggcgg ccagctcctg
     2821 ctgcttgtgg ataacctgaa cgaaaaggaa ccgtccccgg ccgttcaggt gccggtagcc
     2881 tggtgcgcca tggcccggac gggtcggcgg gaggtggtag atggcatcgc tgacgcccgc
     2941 actgaccgcc tgggtacgcg aactcgccgc cgatcaggat gcccggatca ccgccaccac
     3001 tccggtcgtg ccgctggtga cccgaggcct gcctacccgt ccgctcgcgc cggagcactc
     3061 ccaggtgtac gtgtgtgccg gggtgatcgt gaaactgcac ggcaccggga ccgacgcgca
     3121 gcgactcacc cggcggctcg ccggcctggc cgctccggat atggaccggc tctggatcca
     3181 gccgctgcgt taccgtccga ttccggcccc ggacggacgg gtggcgtcga tctggccgcg
     3241 ggtcagcgtg ctgagcacgg tcgaccgtcc gccctggccg gaggtgggca ctttgctggc
     3301 ccggctacat cggatgccga tcggcggtga tctgcctcgg ctgggcggac actcgcggct
     3361 cgcgcgggcg gtggtcttcc tgcgcacgct cggcgctgag tgggcctggc tggtcaacct
     3421 gggcgagcac ctggccgacg cgctggccag gccagggccg aacacgctcg cacacggcga
     3481 tttccatctt ggccagcttg gtcacacccc actgcgccgc agctggaagc tgctggacgt
     3541 cgacgacttc ggcctgggcg acccggcctg ggacctggcc cggccagccg gattctgggc
     3601 agccggcctc ctcgctgacg aagcttgggc gaccttcctg gccgcgtacc gcgaagccgg
     3661 tggcccggcc gtaccggcgg acggggatcc gtggccggcc ctcgacctgc cggcgcgggc
     3721 cgctgtgctg gtggcgaccg tccaggcgct tcggctgtcc cacggcgaac ccacagtcga
     3781 gccattgctg gcggcttgtc ggcgcatgca gactgggacg catgacccat ccgagtgacc
     3841 caccccgcta cgagccgtac gcgggaccgc aggccgcagc cggggccatg ctctgcccga
     3901 agtgcggcgg cccgatgcgc acctacgaac gcaacggaat tcatctggag cagtgctcag
     3961 gctgccgggg agtgttcctg gacttcggcg aactggagca tctcacccag ctggaatcgc
     4021 ggttcgcgca gcagccacct ccacagccca actacggacc ggcctggggc agctacggca
     4081 accagaagta tcgccgtggt ggcttatccg ggttgttctt ctccagctga tcggttcggt
     4141 gagcggacgg aaggtcgtct cgactgctaa cttcgcgggc ccctgcccaa tcaccgcaaa
     4201 ctggacattt ccggccgaca tcggctggaa atgtccagtt tgcgcttggt gaggggatca
     4261 gccgggccgc attccgcgtc cggccgacaa atggtcagtc gaaaccaccg tcgaaaccgt
     4321 cgaaaccacc ccagccgccg tcgccgccgt caccaccacc gccccagtcg atcccgccgg
     4381 agtctgcgcc gatgtcggag ctgtccaagc cggcgtccac accctcgagt tcgccgccga
     4441 agccttcacc caggttgtcg agaaggccga aatccgaagt gagcaggtcg accagcagca
     4501 gcccggtgag cgcgtcatcg agcgccagcc acccgaagcc ggcaagctct tcgccggcag
     4561 ccagatcggc gcggagggca ttcgcgaaca ccgtccgcca gtggtcgacc acgccgagca
     4621 ccatcgcgta cggcaggtaa cggccgaaca cgtccacgcc cacctcgaag cgcagttgct
     4681 ccgcctcggc ggtggccaag tactgccgaa agccgagggt ctggacgcgc gcggcgtagc
     4741 cttccgctga cagcggctgg cccagtccgc gggtgcccgc gactgcgatc acacccgcgg
     4801 cgagagcgcc caaccctgcc cagatcaggc ccgtcgagat caggaacgta ccgaggagga
     4861 gtgcgatcca gccgaccgag cgcacccaac gagcgctcgc cggacggaac cagccatgct
     4921 cagcgctctc ctcgcccagt agtcgcgccg cctcctgcag ccggtggccg gcacgaagct
     4981 cggcgactgt gctcaccggt ccgttgccga aggcggcggc gagcacggcc tgctcagtgc
     5041 gggtcaaagc ctcagcgggc ggattcggca cctggtgcaa ctcccagccg ttcggacggg
     5101 tctggctacc gtccggcgcg atcggcttga gcaccagcca gccgcgaatg gcgaggtcga
     5161 tcagcgttgc ggacagttcg accgcgtcga ctcgtgaatc gatgatcacc cccgctaacc
     5221 ctggccgcag cccagtgggc ggttcaaagc gcaccgcgat cggtggttgc tcgccacggc
     5281 tcagcaagcg gcgctcgctc ggttggccgg gagcaggcag caggccgggg gtgacgccgg
     5341 cgaacacctc gtcgcggctc tccctgcgcg cccagatcaa ccccgcgacg c
//
LOCUS       DAOPRQ010000116         5356 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_12465_length_5356_cov_13.879645, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000116 DAOPRQ010000000
VERSION     DAOPRQ010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 5356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5356
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12465_length_5356_cov_13.879645"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="PKJ61_11510"
     CDS             complement(<1..513)
                     /locus_tag="PKJ61_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HOC14489.1"
                     /translation="MNTLSDDDLAAVSSPGVGPHPQPWPADPRFDRELLAAGDRRNVP
                     DEYRYWTVPAIVAELDARRLPLHVAIQNWEHDFNIGSIVRTANAFNVSGVHIVGRRRW
                     NRRGAMVTDRYLQVHHHANEVALLAWLAERGIVPVGVDNIPGSVPLESSELPAKCCLI
                     FGSEGPGLTDA"
     gene            539..1249
                     /locus_tag="PKJ61_11515"
     CDS             539..1249
                     /locus_tag="PKJ61_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HOC14490.1"
                     /translation="MNLLLQLAVVPLLLPDWLDPEFILTSLGNWALWGAALIIFIECA
                     IFPVLPGDSLLFAVGMFIANDAQIIRFGDLDKPAVLGISIVVLVTAAVLGNVVGYWVG
                     KSISPWLFKPRPGFIGKVFSAKHLDQTHAFFERYGSKALVLGRFVPFVRTFITMVAGA
                     AGMTFRTFITWTGIGAVVWAAGCTLLGYFLGGFDFIKHNLDAVLVAIVLVSVLPMVVE
                     ALLERRRRKSAEVESVES"
     gene            1314..2183
                     /locus_tag="PKJ61_11520"
     CDS             1314..2183
                     /locus_tag="PKJ61_11520"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HOC14491.1"
                     /translation="MPGAPDTRPPATQAELDAAVSALSGRPIAALTGAGFSTDSGLPD
                     YRGPDALPRRPMTYEQFVGSAEFRRHYWARNHLGWHGMHRSEPNDGHYALAELERSGN
                     VLGVITQNVDRLHQRAGSRRVIDLHGHYDLIRCLSCGWTCSRAELDALLLALNPDFLD
                     RVRQLGDIEIAPDADAVLESTAGFVVADCPRCGGVLKPDITFFGESVPRERVAHAFEL
                     VDEAQALLVAGTSLAVMSGLRFVRRAAQRAIPIVIVNRGLTRGDELASVRLNAGVTPA
                     LTYLARRLPPTRS"
     gene            complement(2229..2384)
                     /locus_tag="PKJ61_11525"
     CDS             complement(2229..2384)
                     /locus_tag="PKJ61_11525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HOC14492.1"
                     /translation="MDELDDSEDDGDTAVLAMTGATAMGAGVVGLGLFGLGLLAVLFT
                     RRRRSEA"
     gene            complement(2368..3030)
                     /locus_tag="PKJ61_11530"
     CDS             complement(2368..3030)
                     /locus_tag="PKJ61_11530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14493.1"
                     /translation="MKQTYRVSSLRPGIPMPVRRGISVLLAGILALSSVQVAFAADET
                     PAPEPAATVAPSAASAAAEPSAAADEGDKASDAESSPAKSAAPAEPEEPAADASDDAA
                     RSNDTQKADEAAEADDTAKAGDTAKAAKAKPGDTAKADETATPEAEAPAVSAAADQPS
                     ADAEVGAHGGLRPGDCEVPGDCAGTAGRWLPEHRAAEGIRCGQQGVGMFGADPDRGGR
                     TG"
     gene            complement(3280..4086)
                     /locus_tag="PKJ61_11535"
     CDS             complement(3280..4086)
                     /locus_tag="PKJ61_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020390168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOC14494.1"
                     /translation="MGWFFLVLLIIVVGFALYGVSLYNGFVRSRNLIQESWRQIDVEL
                     NRRYDLIPNLVETVRAYAAHERNTLEGITRLRNQAAALAQAGGGAVTQERAAVEEQLS
                     GAVRQLLVSVEAYPELKSNVNFLELQRELTATEDRIAAGRRFYNANVRNYNTQVESFP
                     SNVLAGMFKFEKATYFEVNEAAVRQAPQVSFGEISDRPAGSAPEPSNDQLAAGAAPSA
                     LPNPQAYPQANPVAPPPGEFPAQQPPLYTPPQAQPGPGQQPPTYQQPPQA"
     gene            4245..4673
                     /locus_tag="PKJ61_11540"
     CDS             4245..4673
                     /locus_tag="PKJ61_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012924258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3151 family protein"
                     /protein_id="HOC14495.1"
                     /translation="MTRDNLLPGPEPTRLPAEGADADARAALAAGRNPRQAVPGAPAA
                     SLLWALLAEEALGIGGDPVAAYAYARTGYHRGLDALRRAGWRGAGPIPSDHEPNQGFL
                     RALLALAEAAAAIGEDAEAERCQQFLLDSGTSTAEVAALR"
     gene            complement(4797..>5356)
                     /locus_tag="PKJ61_11545"
     CDS             complement(4797..>5356)
                     /locus_tag="PKJ61_11545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14496.1"
                     /translation="AVAENPRIVAIERATGRTWPDWLAFLESMDAARLDHTRIAEAVL
                     PQLDGVIDSAGWWTQAVTAAYEQHIGRRLPGQRADGTFQTTVSRATGLGMRELMERWG
                     AFAAGDPEVLGMLSGEPRLSGTERRLSWRARAADGSSILITSEPKKDGSSASLIATLA
                     GLPSPEANQGAKATWASILERFLAS"
BASE COUNT          802 a         1882 c         1773 g          899 t
ORIGIN      
        1 cgcatcggtc aggccggggc cctctgagcc gaagatcagg cagcatttcg ccggcagctc
       61 cgacgactcc agcggcaccg agccagggat gttgtccacc cccaccggca cgatcccacg
      121 ctcggccaac caggccagca gcgcgacttc gttcgcgtga tggtgcacct gcaggtaccg
      181 gtcggtgacc atcgcgcccc gccgattcca gcgccgccgg ccgacgatgt gcaccccgga
      241 gacgttgaac gcgttcgccg tccgcacgat cgagccgatg ttgaagtcgt gctcccagtt
      301 ctgaatcgcc acgtgcagcg gcagccgtcg cgcgtccaac tcggcgacga tcgcgggcac
      361 cgtccaatag cggtactcgt ccgggacgtt gcggcgatcg ccggcagcca gcagttcccg
      421 atcgaagcgg ggatcggccg gccagggctg tggatgcggc ccgacccctg gcgaactcac
      481 cgcggccaga tcgtcgtcgc tcagggtgtt cacaggcgtc acggtaccct tgggagttgt
      541 gaacctactt ctccagctgg ccgtggtgcc cctgttgctg cccgactggc tcgatccgga
      601 gttcattctc acctcactcg gcaactgggc gttgtggggc gcggccttga tcatcttcat
      661 cgagtgcgcg atcttcccgg tgctgcccgg cgactcgctg ctgttcgcgg tgggcatgtt
      721 catcgccaac gacgcacaga tcatccgctt cggtgatctc gacaagcccg cggtgctcgg
      781 catctcgatc gtggtgctgg tgaccgcagc ggtgctgggc aacgtcgtcg gctactgggt
      841 cggcaagagc atctcgccat ggctgttcaa accgcggcca ggcttcatcg gcaaggtctt
      901 ctccgccaag cacctcgacc aaacccacgc cttcttcgag cgctatggct ccaaagcctt
      961 ggtgctcgga cggttcgtgc ccttcgtccg caccttcatc accatggtcg ccggcgcggc
     1021 cggcatgacc ttccgcacct tcatcacctg gaccggcatc ggcgcggtcg tctgggccgc
     1081 gggctgcacg ttgctgggct acttcctggg cggattcgac ttcatcaagc acaatctgga
     1141 cgcggtgctg gtggcgatcg tgctcgtctc ggtgctgccg atggtggtgg aagccctgct
     1201 ggagcggcgg cggcggaagt ccgccgaggt cgagagcgtt gagtcctgac ggagccttcg
     1261 atagggcttc gacttcgagc ccggtaggcg ttcgacggag cgcgatcggc tggatgcccg
     1321 gggcgcccga cacccgtccg cccgcgaccc aggcagagct ggacgccgcc gtcagcgcct
     1381 tgagtggacg tccgatcgcg gcgctcaccg gcgccggatt ctccaccgat tccggcttgc
     1441 ccgactatcg cgggccggac gcgttgccgc ggcgtccgat gacgtacgag cagttcgtcg
     1501 gcagcgccga attccggcgg cattactggg cgcgcaacca cctcggctgg catggcatgc
     1561 accgcagcga gcccaacgac gggcactacg cgctggccga actcgagcgc agcggcaacg
     1621 tgctcggggt gatcacccag aacgtcgacc ggctacacca gcgggccggc tcgcggcggg
     1681 tcatcgacct gcacggtcac tacgacctga tccgctgcct cagctgtggc tggacgtgtt
     1741 cgcgcgccga actggacgcc ctgctgctgg cgctcaatcc cgacttcctc gaccgcgtcc
     1801 gccaactggg cgacatcgag atcgccccgg acgcagacgc cgtcctggaa tcgaccgccg
     1861 gttttgtcgt ggccgactgc ccgcgttgcg gtggtgtgct gaagcctgac atcacgttct
     1921 tcggcgagtc ggtcccacgc gagcgggtcg cgcacgcctt cgagttggtg gacgaggcgc
     1981 aggccctgct cgtggccggc acttcactcg ccgtgatgtc cgggctccgg ttcgtccggc
     2041 gagcggccca gcgcgccatc ccgatcgtca tcgtcaatcg tggcctgacc aggggtgacg
     2101 agttggcgtc cgtccggctg aacgcgggcg tgacaccggc gcttacctac ctcgcccgga
     2161 gattgccgcc gacccggtcc tgacgcccct caatagcggc agtgcccggg ccggcggcct
     2221 cgatcggctc aggcctcgct gcgccggcgg cgcgtgaaca gcaccgccaa cagccccagc
     2281 ccgaacaagc cgagcccgac gacaccggca cccatagcgg tcgcgccggt catggcgagg
     2341 accgcggtgt cgccgtcatc ttcactgtca tccagttcgt ccaccgcggt cggggtcggc
     2401 tccgaacatg cccacacctt gctgtccgca ccggatgcct tcagcagcgc ggtgttcagg
     2461 aagccaccgg cctgcggtcc ctgcgcagtc accaggcact tcgcagtccc ctggtcgaag
     2521 gccgccgtgg gcaccgacct cggcgtccgc gctcggctgg tcggcggcgg ccgacacggc
     2581 cggcgcctca gcctcaggag tggcggtttc gtccgccttc gcggtgtcac cgggcttggc
     2641 cttcgcggcc ttcgccgtgt caccggcctt cgcggtgtcg tcagcttcag cagcctcgtc
     2701 tgccttctgg gtgtcgttgg accgggcagc gtcgtcggac gcgtccgcgg caggctcctc
     2761 gggttcagcc ggcgcggcgc tcttcgctgg gctgctctcc gcgtcgctgg ccttgtcgcc
     2821 ctcgtcggcc gctgcgcttg gctcggcggc cgcgctcgcg gcggaggggg ccaccgtggc
     2881 cgctggctcc ggcgccgggg tctcgtcggc ggcgaaggcc acctggacgc tgctcagcgc
     2941 aagaattccc gccagcagta ctgaaatgcc acgccgcaca ggcataggta tgcccgggcg
     3001 cagcgaactt acccgataag tttgcttcat tgacttgtcc cctccgtcgc gagcaccatc
     3061 cccccagacg ggcgcacgag gcctctagtt aagcacatga ggaaaacccg ctgcagccgc
     3121 ggcctcaagg tcattgatgc tcacaaatgc gactacggcg gaactgccgc caaacgcaag
     3181 aaaagaccgc gctgcggcta gctcaggtgc tagtcacagc gcggtccggg tcgaataaaa
     3241 aaaccccgag gaatcgtgcc gaaaggcgaa ttcctaagcc tacgcctggg gcggctgctg
     3301 gtacgtcggc ggctgctggc cgggcccggg ctgggcctgc ggtggcgtgt acagcggcgg
     3361 ctgctgagcc gggaactccc ccggaggcgg cgcgaccgga ttggcctgcg ggtacgcctg
     3421 cgggttgggc agtgcggacg gggccgcgcc ggcagcgagc tggtcgttcg aaggctccgg
     3481 ggccgagccg gccggccggt cactgatctc gccgaaactc acctgcggcg cctgtcggac
     3541 agcggcctca ttcacctcga aataggtggc tttctcgaac ttgaacatgc cggccagcac
     3601 attcgacggg aacgactcca cctgagtgtt gtagttgcgc acattcgcgt tgtagaagcg
     3661 gcgtccggcc gcgatgcgat cctcggttgc ggtgagttcg cgctgcagtt caaggaagtt
     3721 cacattgctc ttcagctcgg gatacgcctc gacactgacc agcaattggc gaaccgcgcc
     3781 gctcaactgc tcctcaactg cggcacgctc ctgggtcaca gccccaccac cggcctgagc
     3841 gagcgcggcc gcctggttgc gcagccgggt gatgccttcg agggtgttgc gttcgtgcgc
     3901 ggcgtaggcc cggacggtct cgacgaggtt ggggatcagg tcgtagcggc ggttgagctc
     3961 gacgtcgatc tgtcgccacg actcctgtat caggttccgt gagcggacga acccgttata
     4021 gagcgagacc ccatacaggg caaacccgac aacgatgatc agtagcacca ggaagaacca
     4081 ccccatgatc acagactcca atccgagaag ggccgggcgc accggctgcc cttcagccta
     4141 acggtcggca ctgactatgc ggcagcccgt ccgcttcgtt tctggccgtt cctcgctgcg
     4201 gcggtacctg gtgccacgcg aaaccgtccg ggcagactgg ggcgatgact cgcgacaacc
     4261 tacttcccgg ccccgagccc acccgcctgc cagccgaagg agcggacgcg gacgcacgcg
     4321 ccgctctggc cgccggtcgc aatccgcggc aggcggtgcc gggagcgccg gcggcatcgc
     4381 tgctgtgggc gctgctggcc gaggaagcgc ttgggatcgg cggcgatccg gtggccgcct
     4441 acgcctacgc gcgcaccggc taccaccgcg gtctggacgc gctgcgccgc gccggctggc
     4501 ggggcgccgg gccgatcccg tccgatcacg aacccaacca gggcttccta cgcgcactgt
     4561 tggcgctcgc cgaagccgca gcggcaatcg gtgaggacgc cgaagctgag cgctgccagc
     4621 agttcctgct ggactcaggc accagcaccg ctgaggtggc cgcgctccgc tgagtttccg
     4681 agcgcttccc gagcttggtt ccccaacgct ccctgagccc agttcctcaa cgttccctga
     4741 gccggacgaa gggcggtctc gacaagctcg accagccgag gaaccgaccg cagcaatcag
     4801 gaagccagga aacgttccag gatcgacgcc caagtcgcct tggcgccctg gttggcctcg
     4861 ggtgagggca gtccagcgag cgtggcgatc agactggccg acgagccgtc cttcttcggc
     4921 tcgctcgtga tcaggatcga cgatccgtcg gcagctctgg cccgccagga cagccggcgc
     4981 tcggtgccgc tcagccgcgg ctcaccggac agcatcccga gcacctccgg gtcgcccgca
     5041 gcgaacgctc cccagcgctc catcagttcg cgcatcccca agccggtggc tctactgacg
     5101 gtggtctgga aggtgccatc ggcgcgctgt ccaggcagcc ggcgtccgat gtgctgctcg
     5161 tacgcggccg tgacggcctg cgtccaccag ccagcgctgt cgatgacccc gtcgagttgc
     5221 ggcagcaccg cttcggcgat gcgggtgtgg tcgagccgcg cggcgtccat cgattcgagg
     5281 aaggccagcc agtccggcca ggtacgtccg gtggcgcgct cgatcgcgac gatcctgggg
     5341 ttctcagcca cggccg
//
LOCUS       DAOPRQ010000117         5219 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_12868_length_5219_cov_12.073974, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000117 DAOPRQ010000000
VERSION     DAOPRQ010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 5219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5219
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12868_length_5219_cov_12.073974"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1299
                     /locus_tag="PKJ61_11550"
     CDS             <1..1299
                     /locus_tag="PKJ61_11550"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOC14497.1"
                     /translation="LRPRGEAGRGVRRAAFALARLVKDGMFGAVPTSQAAIAVSNLGK
                     TYRLGAMGERPSTAAQAAWTWLRSTGRANFTLFDALNEVSFEVAPGEALGIVGRNGAG
                     KSTLLKILTRVTAPSRGQIELRGRVGSLLEVGTGFHPELTGRENIFLNGAILGMTRKE
                     IQRRYDEIVDFSGIEKFLATPVKRYSSGMYVRLAFSVAAHLDTEILAIDEVLAVGDAE
                     FQRRSIQKMRDAASQGRTVLYVSHQLQTVQALCSSALLLDRGRLIYSGTVEGTLEAYR
                     DSFESYALAQADPDNRPGDGRLRLSSVAMADEFIKSADPIVIEFAAPAQPKLIGSYFI
                     SIHVNNDQGLVIAQCDSRLTGFWLDPAVPQNGRIVIRDLWLKPGAYTVDAYVCQAGVL
                     DAWEGAWRFEVLPDLPYPEFTEASAIEKGMVFTNYSYESR"
     gene            1301..2131
                     /locus_tag="PKJ61_11555"
     CDS             1301..2131
                     /locus_tag="PKJ61_11555"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOC14498.1"
                     /translation="MDTELQRTVIDPPGALNLPSIRELWGAREVAVRLAQRDIIVRYR
                     QTFFGIAWVLLQPLVSAGIFTIVFGQIAGLSTGSTPAFLFTLAGMLAWNLFNGALGRA
                     SSSMVGNQSLVQKVFFPRLLVPLSSLASVALDFLVGLSLAVILLFVYGINPGWQVLLL
                     PVWILLILLLGTGFGLAAAAYMVKYRDVGYVLPWLVQVLLYASPVAYALTEVPATLRW
                     LFDLNPVTWFLEAFRWSLLGSTPPISWQVVALIVVSPLVFLLGTLIFQQNEREFADYI
                     "
     gene            2131..2973
                     /locus_tag="PKJ61_11560"
     CDS             2131..2973
                     /locus_tag="PKJ61_11560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14499.1"
                     /translation="MRVTAYVLIADPNFLAASLGSYYRWVDRIVLSYDRTATSWTGTP
                     LPVAECLDVVAAVDTEGKCVHAPGDYARLDHGPLENDTYQRQAALLAASEGADWVLQL
                     DTDEVMLSPETFFASLNRADEVGAAALEYPSRWLYARVGPGRYLEASSRLGGVAASYP
                     GPMAVRSGTRLQLARQAAVPSYRVDFRFRNTDPANRHDAPVHEVVAESAAILHFSWVR
                     DHDTMRRKFGWSGHTEAYSQPQVFRRWAARQLHPRRTVLTSALRGSDWFRLVDVPEPP
                     GGEP"
     gene            2973..3917
                     /locus_tag="PKJ61_11565"
     CDS             2973..3917
                     /locus_tag="PKJ61_11565"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOC14500.1"
                     /translation="MLSASVVVTCADTSPPLAECLAGLVNQEPRPAEVIVVHRRGGAE
                     IVGLRAEFPDVRFVEFEGKTDAARNAGLARAGAEVVAFIDSDCVAQPGWLASLLENYR
                     NPAVAAVGGRLVEPSGGTNTLIGLALPNGTLATGFDRDPGRTIQVEHLSPANASFRRE
                     SLDAAGGFGSHYAGDGGTGVTELCLRLHAGGQRICFAPGAVVRKPAADRVPPFWPDLR
                     ADYRVRRDHVLLLALVHGTSAKVVRNYLTVAVREEFDCFARMRARSGPVMADGTPRLL
                     SRRLTAPVVVGRGLAGVTGLVAGLAVAVKANAGRKRGG"
     gene            3910..5091
                     /locus_tag="PKJ61_11570"
     CDS             3910..5091
                     /locus_tag="PKJ61_11570"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOC14501.1"
                     /translation="MAELRPLVHAITPGDHFSPLTGSAVPTVVDGLSRAIIDRPARVL
                     VARGTYAERYDSAEAVEYEPAAARRSDRVLDLARSRLGLSRVGTKRQWAAALAGQGGW
                     DPSVIVAHNGPELIPLIDTGRHQAVLYAHNQLLATYGRREAGRCLDPVDAILCVSSYL
                     AEATAERLPKRLCDRLCVVPNGVDANHFAASGRPARGAELRVIFVGRVVPDKGVHVLL
                     EAVRRLGRKDLAVTIVGRPGFAAEAPLSRYEQELRRTARSVPGRVRFASFVERSALPS
                     VLHKADVAVVPSVWPEPFGLTVLEAMAAGAATIASRIGGIPEAGGDAAILIAPGDSAE
                     LATALEIFLEDDQKLAVAQAAAVAHAQRCSWTRSAESLSAALAAIEGGGCDERWAVGT
                     A"
BASE COUNT          772 a         1664 c         1829 g          954 t
ORIGIN      
        1 cttaggcccc gcggagaggc cggtcgcggg gtccgacgag cggcgttcgc cctggcccgg
       61 cttgtcaaag acggtatgtt cggtgccgtg ccaacgtccc aagcggccat agccgtctcg
      121 aatctgggca agacctaccg tctcggcgcg atgggagaac gtcccagcac cgcggcgcag
      181 gcagcctgga cctggctgcg atcgaccggt cgggcaaatt tcaccctctt tgacgccctc
      241 aacgaggtct ccttcgaggt cgcaccgggt gaagcgctgg gaatcgtggg ccgaaacggc
      301 gctggcaagt ccaccttgct gaagatcctc acccgcgtga ccgcaccgag tcgcggacaa
      361 atcgagttgc ggggtcgggt cggatcgctc ttggaggtgg gcacgggttt ccatcccgaa
      421 ctcaccggac gcgagaacat ctttctcaac ggtgccattc tggggatgac gcgaaaggag
      481 atccagcggc gctacgacga gatcgtcgac ttctccggga tcgagaagtt tctggctact
      541 ccggtaaagc ggtactcctc cggcatgtat gtccggctgg cgttctccgt ggcagctcat
      601 ctggataccg agatcctagc catcgacgaa gtgctggccg ttggtgatgc ggaattccag
      661 cggcgctcga tccagaaaat gcgtgacgcg gccagtcagg gacgtacggt tctctacgtg
      721 agccaccagc tgcagacggt gcaggcactg tgcagttcgg cgttgctcct ggaccgcggc
      781 cggctcatct actccggtac cgtggagggg accctggagg cataccggga cagtttcgag
      841 agctacgcgc tcgcccaggc ggacccggat aaccggccag gcgacgggcg gctccgcctg
      901 tcgtcagttg caatggccga tgaattcata aagtccgctg atccgattgt gatcgagttt
      961 gctgcaccgg cacaaccgaa gctgatcggc agctacttca tctcgatcca tgtcaacaat
     1021 gaccagggcc tggtcatcgc ccagtgtgac tcgcggctga ctggcttctg gctcgacccg
     1081 gctgtccccc aaaacgggcg gatcgtgatc cgtgacctgt ggctgaaacc cggggcctac
     1141 acggtcgatg cctatgtctg ccaggccgga gtgctggacg cctgggaggg cgcttggcgg
     1201 ttcgaggtgc ttcctgacct gccctatccg gagttcacgg aagcctcggc catcgagaag
     1261 ggcatggtct tcaccaacta cagctacgag agccgctgac atggacaccg aactgcagcg
     1321 aaccgtgatc gatccccccg gggcgctcaa cctgccctcg atccgcgagt tgtggggcgc
     1381 ccgcgaggtc gccgtgcgct tggcgcagcg ggacatcatc gtgcgctacc ggcagacgtt
     1441 cttcggtatc gcgtgggtgc tgctgcagcc gctggtgagc gccgggatct tcacgatcgt
     1501 tttcggtcag atcgcagggc tgtcgaccgg gtccaccccc gcgttcctgt tcacgctcgc
     1561 aggaatgctc gcctggaacc tgttcaacgg ggcgctcgga cgggcgtcct cgtcgatggt
     1621 gggcaaccag tcactcgtcc agaaggtgtt cttcccgcgc ctgttggtcc cgctgtccag
     1681 tctcgcttcg gtggcgctgg acttcctggt gggattgtcg ctggcggtga tcctcctatt
     1741 cgtctacggc atcaaccctg gctggcaggt gctgctgctg ccggtctgga tcctgctgat
     1801 cctgttgctg gggactggct tcgggctggc agctgccgcc tacatggtga aatatcgcga
     1861 tgtgggctac gtgttgccct ggttggttca agtgctgctc tatgcgagcc cggtcgccta
     1921 cgccctcacc gaggtgccgg ccacgctgcg ctggcttttc gacctgaacc cggtcacgtg
     1981 gttcctggaa gcgttccggt ggtcgttgct cggcagcacc ccgccgatca gctggcaagt
     2041 ggtcgcgctg atagtggtgt caccgctggt cttcttgctc ggcactctga tcttccagca
     2101 gaacgaacgc gagttcgcgg actacatctg atgcgagtca ccgcgtacgt cctgatcgcc
     2161 gacccgaact tcctcgccgc gagcctgggg tcgtactacc ggtgggtcga ccggatcgtg
     2221 ctctcctacg accgcaccgc gacgtcgtgg acgggaacgc cgctgccggt ggccgagtgc
     2281 ctggacgtgg tggccgcggt cgacaccgag ggcaagtgtg tgcatgctcc gggcgactac
     2341 gcacggcttg atcacggccc gttggagaat gacacctacc agcggcaggc agccctgctg
     2401 gccgcttccg agggcgccga ttgggtgctg caactcgaca ccgatgaggt gatgctcagt
     2461 ccggagacgt tcttcgcttc cctgaacagg gcagacgaag tgggtgctgc ggcgctggag
     2521 tacccctccc gatggctcta cgcacgggtg gggccgggac gctatctcga ggcaagtagc
     2581 cggttggggg gcgtggccgc gtcctacccg gggcctatgg ccgttcggtc agggacgcga
     2641 ctgcaactgg cgaggcaggc cgcggtgccg tcctaccggg tcgacttccg gttccgcaac
     2701 accgacccgg ccaaccgaca cgacgcgccg gtccacgaag tggtggcgga gagcgctgcg
     2761 atcctgcact tctcctgggt gcgcgaccac gacaccatgc gacgcaagtt cggttggtcg
     2821 gggcacaccg aggcctactc tcaaccgcag gtgtttcgcc gctgggccgc gcgccagttg
     2881 catccgcgcc ggacagtgtt gacctcggcc cttcgcggct cggactggtt ccggctggtc
     2941 gacgtgcccg aaccgccggg gggtgaaccc tagtgctgag cgccagcgtg gtggtgacct
     3001 gcgccgacac gtccccgccg ttggcggaat gcctggcggg cctggttaac caggaacccc
     3061 gcccggcaga ggtcatcgtc gtccatcggc gcggtggggc cgagatagtc ggcctgcgag
     3121 cggagttccc cgacgtgcgc ttcgtcgagt tcgagggcaa gacggacgcg gcccggaatg
     3181 ccggcctggc tcgcgctggg gcagaagtcg tggccttcat cgacagcgac tgcgtggctc
     3241 agccgggctg gctcgcgtcc ctgctggaga actaccgaaa cccggcggtg gccgccgtag
     3301 gcggccggct ggtggagcca tccggcggga ccaacacgtt gatcgggctt gcgctgccca
     3361 acggaacctt ggcgaccggc ttcgaccggg atccgggccg gacgattcag gtggagcacc
     3421 tcagcccggc caacgcgtcc tttcgcaggg agtctctcga cgccgcgggt gggttcggta
     3481 gccattacgc cggtgatgga ggcactgggg tcaccgaact ctgtctgcgg ctacacgcgg
     3541 gcgggcagcg gatctgtttc gctcccggcg cggtcgtccg gaagccggcc gcggaccgcg
     3601 tgcccccctt ctggccagac ttgcgcgccg actatcgcgt gcgccgcgat cacgtgttgc
     3661 tgctcgccct ggtgcacggc acgagtgcga aggtggtgcg gaactacctg accgttgcgg
     3721 ttcgggagga gttcgactgc ttcgccagga tgcgtgcgcg aagtgggccg gtgatggcgg
     3781 acggcacgcc ccgactgctt agccgacgcc tgaccgcgcc ggtggtggtg ggtcgcgggc
     3841 tggccggcgt gaccggtctg gtcgcggggc tggccgtggc agtcaaggcg aatgcgggcc
     3901 ggaagcgggg tggctgagtt gcggccgctg gtgcatgcga tcaccccggg ggatcacttc
     3961 tcgccgctca ccggcagtgc agtcccgacc gtggtcgacg ggctcagccg agccatcatc
     4021 gaccgcccgg cgcgcgtgct ggtggcacgt ggcacctacg ctgagcgtta cgactcggcc
     4081 gaggccgtcg aatacgaacc ggccgcagcc cggcgctccg accgggtcct agacctcgct
     4141 cggtcccggc tcgggttgtc ccgagtgggg acgaagcggc agtgggcggc ggccctggca
     4201 ggtcagggcg gctgggatcc ttcggtgatc gtcgcgcaca acggccctga gctgatcccg
     4261 ctgatcgaca ccggacggca ccaggctgtt ctctacgcgc acaaccaact gctggcgacc
     4321 tatggtcggc gcgaggccgg ccgatgcctc gacccggtgg acgcgatcct ttgcgtcagc
     4381 tcttacctgg ccgaggcgac cgccgagcgg ctcccgaagc gcctttgcga tcgactttgc
     4441 gtggtgccta acggggtcga cgccaaccac ttcgccgcct cggggcgccc tgcccggggt
     4501 gcggagctgc gggtgatctt cgttgggcgg gtggtgccgg acaagggcgt gcacgtgctg
     4561 ctcgaggcag tccgccggct tgggcgcaag gaccttgcgg tcaccatcgt gggtcggcca
     4621 ggattcgccg cggaggcccc gctgtcccgt tacgagcagg aactgcggcg aaccgctcga
     4681 tcggtgccgg ggcgggtgcg gttcgcttcc ttcgtcgaac gcagcgctct gccgagcgtg
     4741 ttacacaagg cggacgtggc cgtggtgccc tcggtgtggc cggagccctt cggcctgacg
     4801 gtgctggagg cgatggcggc gggggcggcg accatcgcgt ctcggattgg tggcattccc
     4861 gaagccggcg gcgacgctgc catcttgatc gcgcctggcg attctgccga gttggctacg
     4921 gccctcgaga tcttcctgga agatgatcag aagctcgcag tcgcccaggc cgctgcggtt
     4981 gcccacgcgc agagatgctc atggactcgg tccgcggaga gcctgtcggc agcattggcg
     5041 gccattgaag ggggtggatg cgatgagcgg tgggcggtcg gcacggcttg attcccggac
     5101 cgtcgacctg gcggcattga ctgaagtcga cgttgcccgg tgggcggatc tggcggggcg
     5161 cgcactagga gcctgctgaa caaggcgcca ttatgagggc gtatcgagcg tggcgacgg
//
LOCUS       DAOPRQ010000118         5108 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_13213_length_5108_cov_11.733030, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000118 DAOPRQ010000000
VERSION     DAOPRQ010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 5108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5108
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13213_length_5108_cov_11.733030"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            199..1143
                     /locus_tag="PKJ61_11575"
     CDS             199..1143
                     /locus_tag="PKJ61_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015071072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOC14502.1"
                     /translation="MSSHGGTKAVVAAMAANAFIAVTKFAAWGLTGAASMLAEGVHSV
                     ADTANQILLLVGGRSAKREASEEHPFGYGRARYINAFLVAIILFSLGGLFALYEAFHK
                     YEEVIGGHPNELLEGPWWWVPIVVLVAAIIAEGFSFRTAIKESIPAKGRQSWPRFIKT
                     SKSPELPVVLLEDFAALLGLVFALMGVGMTLLTHNGLWDAVGTALIGILLIAVAITLA
                     IETRSLLLGEAATPEAVVAIRSALEQADGVGRVIHLRTLHLGPDEVLVAAKIAVEPTE
                     SAQRVAQVIDNAEIAIRAANSNVTALYLEPDIDRGPEL"
     gene            1302..2717
                     /gene="ahcY"
                     /locus_tag="PKJ61_11580"
     CDS             1302..2717
                     /gene="ahcY"
                     /locus_tag="PKJ61_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HOC14503.1"
                     /translation="MEYRVNDLNLAPFGRAEIKLAEHEMPGLMALRERYGDAKPLRGA
                     RIAGSLHMTVQTAVLIETLIALGAEVRWASCNIFSTQDHAAAAIVVGPDGTPDDPRGV
                     PVFAWKGESLEDYWWCTERILDWGDRTPNMILDDGGDATLLVHKGVEFAKAGAVPDAS
                     ASDSAEYQVVLARLRESTLDWVGIANSIFGVTEETTTGVHRLYEMARNQSLLFPAINV
                     NDSVTKSKFDNRYGIRHSLIDGINRATDVLIGGKTAVVCGYGDVGKGCAAALAGQGAR
                     VVVTEIDPICALQAAMDGFQVATLDDVVSTADIFITATGCLKVITADHLAKMKHQAIV
                     GNIGHFDNEIDMAGLEALPDVNRVNIKPQVDLWEFPDGHSIIMLSEGRLLNLGNATGH
                     PSFVMSTSFANQVLAQIELYTKPEQYPVGVYTLPKQLDEYVASLHLGSLGVHLSELTE
                     EQADYLGVAVEGPFKTELYRY"
     gene            complement(2776..2925)
                     /locus_tag="PKJ61_11585"
     CDS             complement(2776..2925)
                     /locus_tag="PKJ61_11585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14504.1"
                     /translation="MNVAKAGKPKRITLLSSSRLEETLKPNLNKCGNPMKPAYDEVKA
                     LLTPA"
     gene            complement(3209..3706)
                     /locus_tag="PKJ61_11590"
     CDS             complement(3209..3706)
                     /locus_tag="PKJ61_11590"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOC14505.1"
                     /translation="MAGKTLIVLRHAKSSWHTDAPDLRRPLSERGRRDAVVAGGMLAD
                     YHIDVVLASTAVRVQQTWERAQLGGAECADVRSSEAIYHAWPGELLAELHELDESLHT
                     ALLIGHQPTLGDLITTLAKPSALADQVVRKFPTSGLAVLTYRGAWKTLSAGKATLKRF
                     EVPRG"
     gene            complement(3721..3987)
                     /locus_tag="PKJ61_11595"
     CDS             complement(3721..3987)
                     /locus_tag="PKJ61_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012919432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3039 domain-containing protein"
                     /protein_id="HOC14506.1"
                     /translation="MTTEQPQTITIVKERTEERTDLRPEEGDHERFSHYVPKAKLTEA
                     MVMGTPVIALCGKVWVPSRNPDRFPVCPECKEIWASLRPGEGDN"
     gene            4085..>5108
                     /locus_tag="PKJ61_11600"
     CDS             4085..>5108
                     /locus_tag="PKJ61_11600"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOC14507.1"
                     /translation="MTTPGETQAQAHSRRGFVVGILLCVVATAFEAMAVLTAMPAAAK
                     DLGGHDYYAWTFTGFVIAQTFAIVAAGLACDRFGPVRPLMIGFGLFTVGVVLAAMSPT
                     MPLLIGARVVQGLGGGTMNLAIMVLVGLVFDGHERAVMMTGFSFAWMAPSFVGPPVAA
                     WLSENLSWHWVFWSVLPLLAVSGALSARPLLRLPLPDLAPADVQTRRFLASAGMVSIG
                     AALVQWAGQHIELLSLLWLAMGLVLLWFGLRPLMPAGYALAGRGLAAVINVRSALAGA
                     FFGAQAFLPLMLTEARGLELQWAAWTMTIGSLGWTFGSWLQSRRWLRMRRDAIITAGA
                     LSLGVGL"
BASE COUNT          773 a         1667 c         1753 g          915 t
ORIGIN      
        1 gtgtaagccg ggatctccga gtcagtccga gatcccggcc tacgccggga tgacgtgtgt
       61 gtccgggatg acgtgtgtgt ttccgggatg acgtgtgtgg gtccggagat cggccagttc
      121 gactgtcggc cctgaacgag aaggggagtg tccccggacg tactgggacg cgagggcggc
      181 tctggcagga tgtcgggcgt gagtagtcat ggtggaacca aggcggtcgt cgctgcgatg
      241 gcggcgaacg cgttcatcgc ggtcaccaag ttcgcggcgt gggggctgac cggggccgcg
      301 tccatgctgg ccgaaggcgt gcactcggtc gcggacacgg cgaaccagat cctgctgctg
      361 gtgggtggaa ggtcggccaa acgcgaagcc agcgaggagc atccgttcgg ctacggccga
      421 gcgcggtaca tcaacgcctt cctggtggcg atcatcctgt tcagcctcgg cggcctgttc
      481 gccttgtacg aggccttcca caagtacgag gaagtgatcg gcggccaccc caacgagttg
      541 cttgaggggc cgtggtggtg ggtgccgatc gtcgtgctgg tcgcggcgat catcgccgaa
      601 gggttcagct tccggactgc gatcaaggag tcgatcccgg ccaaggggcg gcagagctgg
      661 ccccggttca tcaagacctc caagtcgccg gaactgccgg tcgtgctgtt ggaggacttc
      721 gccgcgctgc tgggcctggt cttcgcgttg atgggcgtcg gaatgacgct cctcacccac
      781 aacgggctct gggacgcggt cggcaccgcg ctgatcggca tcctgctgat cgcggtggcg
      841 atcaccctgg ccatcgagac ccgcagcctg ctgctgggtg aggccgccac cccggaggcg
      901 gtcgtcgcga tccgttcggc cctcgaacag gcggacgggg ttggccgcgt catccacctg
      961 aggaccctcc acctgggccc ggacgaggtg ctggtggcgg ccaagatcgc ggtcgagccg
     1021 accgaatccg cccaacgcgt ggcccaggtg atcgacaacg ccgagatcgc gatccgtgcg
     1081 gcgaactcga acgtgaccgc gctctacctc gaacccgaca tcgatcgcgg tcccgaactg
     1141 tagccctggc ggacttcgct cccgaacggg accttcgctc ccgatagttc gggagcggga
     1201 gtccccttcg ggagcgaagt cgaggcattg gcgagcggac gcgtcctcgc gttcgccgtc
     1261 gaatgtcccc ggaccggggt caccgcgtac gatcccgtcc tatggagtat cgcgtaaacg
     1321 acctgaacct ggccccgttc gggcgagccg agatcaagct tgccgaacac gagatgccgg
     1381 gcctgatggc cttgcgggag cgctacggcg acgccaagcc gttgcgcggc gcccggatcg
     1441 ccgggtcatt gcacatgacc gtgcagaccg cggtgctcat cgagaccctg atcgccctcg
     1501 gcgccgaggt tcgctgggcc tcttgcaaca tcttctccac ccaggaccat gccgccgccg
     1561 cgatcgtcgt cggcccggac ggcaccccgg acgaccctcg tggcgtcccg gtgttcgcct
     1621 ggaaggggga gtcgctggag gactactggt ggtgtaccga acgcatcctc gactggggcg
     1681 accggacgcc gaacatgatc ctcgacgacg gcggggatgc caccctgctc gtccacaagg
     1741 gggttgagtt cgccaaggct ggtgcggtgc cggacgcgtc cgcgtccgat tcggcggagt
     1801 accaggtggt gctggcccgg ctgcgcgagt cgacgctgga ctgggtgggc atcgcgaaca
     1861 gcatcttcgg ggtcaccgag gagaccacca ccggcgtcca tcgcctgtac gagatggcca
     1921 gaaaccagtc gctgctgttc ccggcgatca acgtcaacga ctcggtcacc aagagcaagt
     1981 tcgacaaccg ctacggcatc cggcactcgc tgatcgacgg gatcaaccgc gccaccgacg
     2041 tgctgatcgg cggcaagacg gcagtggtct gcgggtatgg cgacgtgggc aagggctgtg
     2101 cggccgcgct ggccggccag ggcgcccgcg tcgtggtcac cgagatcgac ccgatctgcg
     2161 cgctacaagc ggcgatggac gggttccagg tcgccactct ggacgatgtg gtcagcaccg
     2221 cagacatctt catcaccgcc accggctgcc tgaaggtgat caccgcagac cacctggcca
     2281 agatgaagca ccaggcgata gtgggcaaca tcggccattt cgacaacgag atcgacatgg
     2341 ccgggctcga agcgctcccc gacgtgaacc gtgtcaacat caagccgcag gtcgacctgt
     2401 gggagttccc ggacgggcac tcgatcatca tgctcagcga aggccgactg ctgaacctcg
     2461 gcaacgcgac cgggcacccc agcttcgtga tgagcacctc cttcgccaac caggtgctgg
     2521 cgcagatcga gctgtacacc aagccggagc agtatccggt gggggtgtac acgctgccca
     2581 agcagctcga cgagtatgtc gccagcctgc atctcggctc gctcggcgtg catctctccg
     2641 agctcaccga ggagcaggcc gactacctcg gtgtggctgt cgaaggtccg ttcaaaacgg
     2701 agttgtaccg ctactgagcc gcgacgatcg gccaatcagc cgatcggggc cggtcgatca
     2761 ggtcgatcgg gccggtcagg ccggggtcag caatgccttg acctcgtcgt aggccggctt
     2821 catcgggttt ccgcatttgt tcaggttcgg tttgagggtt tcctcgagcc gtgaggagct
     2881 gaggagcgtg attcgcttcg gcttgccggc cttggccacg ttcaccacta cctcgcggta
     2941 gtcccacgtc tgctgccagc cccacttgcc gccgttcggt ttgccgttgg tggtgcactt
     3001 ctcggtgcgg tggcgcagcc gttgcgctgc aaccggtgat gaagaggccg gtaaggatgg
     3061 ccgtggacgc gactgccttg aaccacatgt ctacccacgg aaagggttgt cgttgagccg
     3121 atccagcccg cttgaagcgg ggttcgatca accttgcggc tcacgttagc cccgttgctg
     3181 aggcctcgca ggcgaacggc tgggttgctc agccccgtgg cacctcgaac cttttcaaag
     3241 tggctttgcc cgcagacagg gtcttccagg cgccgcggta ggtgagcacc gccaggcccg
     3301 aggtggggaa cttgcgcacc acctgatcag ctagtgcgga cggcttggcc agggtcgtga
     3361 tcaggtcacc gagggtgggc tggtggccga tcagcagcgc ggtgtgcagg ctctcgtcca
     3421 actcgtgcag ttcagccagc agttcgcccg gccaggcgtg gtagatcgct tcggagctgc
     3481 ggacgtccgc gcactcggcg ccgccgagct gggcccgctc ccaggtctgc tggacgcgta
     3541 cagcagtcga cgcgagcacc acgtcgatgt ggtaatcggc gagcatccca ccggccacga
     3601 ccgcgtcccg ccggccccgc tccgacaggg ggcgacgcag gtcgggcgcg tcggtgtgcc
     3661 aggacgattt cgcgtgccgc agcacgatca gggtcttgcc ggccatgctg cctccacgcc
     3721 tcagttgtcg ccctcgcccg gacgcaacga cgcccagatc tccttgcact cagggcacac
     3781 ggggaaccgg tcggggttgc gggagggcac ccacaccttg ccgcacagcg cgatgaccgg
     3841 cgtgcccatc accatggcct cggtgagctt cgccttcggc acgtagtgcg aaaaccgctc
     3901 gtggtcgccc tcctcgggac gcaggtcggt gcgttcctcg gtgcgctcct tgacgatggt
     3961 gatcgtctgc ggctgttccg tggtcatctg gccctcctgc tcgcgtcgcc ggacaccgtc
     4021 caacctaccc aaagcgcagt ccgcccgaaa ccgcgcccag cacgttgtgg caggatcgat
     4081 caacgtgacc acccccggcg aaacccaggc gcaggcgcat tcgcggcgtg ggtttgtcgt
     4141 cggcatcctg ctctgtgtcg tggcgaccgc tttcgaggcg atggccgtgc tcacggcgat
     4201 gcccgccgca gccaaggacc tcggtggcca cgactactac gcctggacgt tcaccggttt
     4261 cgtgatcgcg cagaccttcg cgatcgtcgc cgccgggctt gcctgtgacc gcttcggacc
     4321 ggtgcgtccg ctgatgatcg ggttcgggct gttcaccgtc ggcgtggtgc tcgcggcgat
     4381 gtcgccgacc atgccgctgc tgatcggcgc gcgcgtcgtg cagggcctcg gcggcggcac
     4441 catgaacctg gcgatcatgg ttctggtcgg gctggtcttc gacgggcacg aacgcgcggt
     4501 gatgatgacc ggcttctcgt tcgcctggat ggcgccgtcg ttcgtcgggc caccggtggc
     4561 cgcctggctg agcgagaacc tgtcctggca ctgggtgttc tggtcggtgc tgccgctgct
     4621 cgcggtctcg ggcgcactat ctgcccggcc attgctgcgg ctgccgctgc cggacctggc
     4681 acccgccgac gtgcagaccc ggcggttcct cgccagcgcg ggcatggtgt ccattggggc
     4741 ggcgctggtg cagtgggccg gacagcacat cgaactgctt tcgttgctct ggctcgccat
     4801 gggcttggtc ctgctgtggt tcggtctgcg tccgctgatg ccggcgggct acgcactggc
     4861 cggacgcggg ttggccgcgg tgatcaacgt ccgctcagcc cttgccggag ccttcttcgg
     4921 ggctcaggcc tttctgccgc tgatgctcac cgaggctcgc ggtttggaac tgcaatgggc
     4981 ggcatggacg atgaccatcg gctcgctggg ctggacgttc ggttcgtggc tgcagtcgcg
     5041 acgctggctg cgaatgcgcc gggacgcgat catcaccgcg ggcgcgctca gcctcggcgt
     5101 ggggctcg
//
LOCUS       DAOPRQ010000119         4986 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_13638_length_4986_cov_10.385926, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000119 DAOPRQ010000000
VERSION     DAOPRQ010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 4986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4986
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13638_length_4986_cov_10.385926"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1530
                     /gene="narH"
                     /locus_tag="PKJ61_11605"
     CDS             <1..1530
                     /gene="narH"
                     /locus_tag="PKJ61_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HOC14508.1"
                     /translation="TEYVWFNNVETRPGLGYPRRYEDQDKWRGGWVRTASGGLKLRSG
                     GRFQKLLSIFASPVQPELDDYYEPWTYDYQNLISAPLGDDFPVARPKSLITGEDTKIT
                     WSANWDDSLGGVHATGDADPIVQKLREQANEQIKFEYEKSFMFFLPRICEHCLNPSCM
                     ASCPSGAIYKRAEDGIVLVDQDRCRGWRQCITGCPYKKIYFNHRSGKAEKCTFCYPRI
                     EVGLPTVCSETCVGRLRYLGIVLYDPDAVTAAAVADEKDLYEAQMDLLLDPNDPEVIA
                     LARAGGMPEDWIAAAQRSPIYALIKHFKVALPLHPEYRTMPMVWYIPPLSPVVDHLAA
                     QGHDAEAAGNLFGAIDALRIPVTYLAELFTAGDTAIVTGVLQKLAAMRAYMRGVTLGT
                     GRDPELAAAVGMTPEALEQMYRLLAIAKYDERYVIPKAHHEEAHNLEELACSLDFEGG
                     PGMYESGPFGEASGRPVPVAIETYTALKARQQAEEAASSDQLQNRTALLNWDANEDAK
                     P"
     gene            1602..2270
                     /gene="narJ"
                     /locus_tag="PKJ61_11610"
     CDS             1602..2270
                     /gene="narJ"
                     /locus_tag="PKJ61_11610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HOC14509.1"
                     /translation="MTAAETTSNVVPAKAGISAATICQAAALVLAYPEAELLDRLDLI
                     ETALIGGPAAEQFAPVLAHLRGETASASDAEDPDELLSLLQAFHVQEFDLSRKHSLHL
                     SYWTDGDTRRRGNVLAEVKQVYRESGLLTDTGGELPDYLPMVLEFTAADPERGFGLLQ
                     RFRASVELIRLGLSKDHLPHAGVLAAVCDLLPGPSPTTRAEVQARFGEVQPIELVGLE
                     LVRS"
     gene            2272..3036
                     /gene="narI"
                     /locus_tag="PKJ61_11615"
     CDS             2272..3036
                     /gene="narI"
                     /locus_tag="PKJ61_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HOC14510.1"
                     /translation="MNALDVLLWGALPYVVVIMLVAGTIWRYKYDQFGWTTRSSQLYE
                     SKLLRIASPMFHYGLFVVLMGHLLGLLIPKAWTDLVVTQETYHMVALVAGTLAGVATL
                     AGIVLLIYRRRTTGPVFLATTRNDKLMYVVLVAAIVFGMIATLTGDQTASGEHHNYRE
                     TVSIWFRSLLVFQPNVEAMAAATWQFKLHILVGFALFALMPFSRLVHAFTVPLHYLFR
                     PYIVYRSRDALTPTPSRGRVRGWDPIGTQDRDRKTR"
     gene            3033..4424
                     /locus_tag="PKJ61_11620"
     CDS             3033..4424
                     /locus_tag="PKJ61_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HOC14511.1"
                     /translation="MTQTTHAAHPHHGDDVVAPIHETGRGANRVLAMSTIDFTVMFAV
                     WLMFGILGIEIQKELLLDDVQLSWITALAVLNGSMWRLPAGMITDRIGGRKVTIFMLA
                     ATAVPAYFVATAHSYGVLLILAFLVGFAGNLFSVGIAWNAAWFGRERQGFALGLFGAG
                     NVGASVTKFIGPPLIAGTAGATFIFGIQGGWRLVPVIYSVLLVVLAVLTWLIVPRHDR
                     LAGESKPIRDMLAPLTHVRVWRFSLYYVAVFGAYVALSAWLPKYYVDNFKVPLYTAAL
                     LTAVFIFPASLLRPLGGWISDRIGARKVMYTTFTVMLATTGILMMPNGHIVITHADGS
                     QTEHLAYSINVWWFTFLVFLLGCAMGVGKAGVYKHIPEYFPDNVGSVGGLVGMLGGLG
                     GFILPPLFAYTKLWTGFPTSTFFVLFILTAICAIWMHLTIVRMLHAQAPQLADHFDTE
                     HIDTISLNEEAKA"
     gene            4421..>4986
                     /locus_tag="PKJ61_11625"
     CDS             4421..>4986
                     /locus_tag="PKJ61_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOC14512.1"
                     /translation="MSGSIVERLQGGPEWLKSWDPENEQTWDKRLAWQTLWITTFTLT
                     LCFVTWFLPSAIVPKLNAIGYSFTREQLYWLAAIPGLSGGLLRLVWMVLPPVMGTRKM
                     VAATTLLLFFPVLGWGVRSMSPTAPYWELLVLAFLAGIGGGAFSGFMPSTSYFFPRNK
                     QGTALGLQAGIGNFGVSLVQLLTPWLIGL"
BASE COUNT          799 a         1723 c         1572 g          892 t
ORIGIN      
        1 accgagtacg tctggttcaa caacgtcgag acccggcccg ggctcggcta cccgcgccgc
       61 tacgaggacc aggacaagtg gcgcggcggc tgggtgcgga ctgcctccgg cggcctcaag
      121 ttgcgtagtg gcggacgctt ccagaagctg ctcagcatct ttgcctcgcc cgtacagccc
      181 gaactggacg actactacga gccgtggacg tacgactacc agaacctgat ctcggccccg
      241 ctcggcgacg acttcccggt ggcccggccg aagtcgctga tcaccggcga ggacaccaag
      301 atcacctggt cggctaactg ggacgattcg ctgggcggcg tccatgccac cggcgacgcc
      361 gacccgatcg tgcagaagct gcgtgagcag gccaacgagc agatcaagtt cgagtacgag
      421 aagagcttca tgttcttcct gccgcggatc tgcgagcact gcctgaaccc gtcctgcatg
      481 gcgtcgtgcc cgtccggggc gatctacaag cgggccgagg acggcatcgt cctggtcgac
      541 caggaccgct gccgcggctg gcgtcaatgc atcaccggct gcccgtacaa gaagatctac
      601 ttcaaccacc gctccggcaa ggccgagaag tgcaccttct gctacccgcg gatcgaggtc
      661 gggctgccga cggtgtgctc ggagacctgt gtcggccggc tgcgctacct gggcatcgtg
      721 ctctacgacc cggacgccgt gaccgcggct gccgtggccg acgaaaaaga cctttacgaa
      781 gcgcagatgg acctgctgct cgacccgaac gatccggaag tcatcgctct ggccagggcc
      841 ggtggaatgc cggaggactg gatcgcagcc gcgcagcgtt cgccgatcta tgccctgatc
      901 aagcacttca aggtcgcgct gccgctgcat ccggagtacc gcaccatgcc gatggtctgg
      961 tacatcccgc cgctgtcccc ggttgtcgac cacctggctg cgcagggcca cgacgccgaa
     1021 gccgccggca acctgttcgg cgcgatcgac gccctgcgga tccccgtcac ctatctggct
     1081 gagctgttca ccgcaggcga caccgcgatc gtcaccggag tgctgcagaa gctggccgcc
     1141 atgcgggcct acatgcgcgg cgtgaccctt ggcaccggac gcgacccgga actcgccgcc
     1201 gccgtgggca tgacgccgga ggcgttggag cagatgtacc ggctgctggc gatcgccaag
     1261 tacgacgagc gctacgtgat ccccaaggcg caccacgagg aggcgcacaa ccttgaggaa
     1321 ctcgcctgtt cgctcgactt cgagggtggc ccgggcatgt acgagtcggg tccgttcggc
     1381 gaagcgtcgg gacgccccgt gccggtcgcg atcgagacct acacggcgct gaaggcccgc
     1441 cagcaggccg aggaggccgc ctcgtccgac cagttgcaga accgcaccgc gctgctcaac
     1501 tgggacgcca acgaggacgc gaagccatga gcccgctgtt ctcccgaagc cgaaaggtcc
     1561 agatcccagg ccttgcccgg gatgacgaag gtggcaccac gatgactgcc gccgaaacca
     1621 cctcgaacgt cgtcccggcg aaggccggga tctccgcagc aacgatctgc caggccgcag
     1681 cgctggtgct ggcctacccg gaagccgaac tgctcgaccg cttggacctg atcgaaaccg
     1741 cgctcatcgg tggtccagcg gccgagcagt tcgccccggt gctcgcgcat cttcgtggcg
     1801 agacggcgtc cgcatccgac gctgaagacc cggacgaact gctgtcactc ctccaggcct
     1861 tccacgtgca ggagttcgac ctgtctcgca agcattcgct gcaccttagc tactggaccg
     1921 acggcgacac gcggcggcgc ggcaacgtgc tggccgaggt gaagcaggtc taccgcgagt
     1981 ccgggctgct gaccgacacc ggcggcgagc tgcccgacta cctgccgatg gtgctcgagt
     2041 tcaccgcagc ggaccccgaa agagggttcg gcctgctgca gcgcttccgc gccagcgttg
     2101 agttgatccg gctcggcctg tcgaaggacc acctgccgca tgccggcgtg ctggccgcag
     2161 tctgcgacct gctgcccgga ccgtcgccga ccacccgcgc cgaggtgcag gcacggttcg
     2221 gcgaggtgca accgatcgaa ctggtcgggc tcgaactggt gaggagttga gatgaacgcc
     2281 ctggacgtcc tgctttgggg agccctgccg tacgtcgtcg tcatcatgct ggtcgccggg
     2341 acgatctggc gctacaagta cgaccagttc gggtggacca cccgctcgtc ccagctgtat
     2401 gagtccaagc tgctgcgaat cgcgtccccg atgttccact acggcctgtt cgtcgtcctg
     2461 atggggcacc tgttggggct gctgatcccg aaggcgtgga ccgacctggt cgtgacccag
     2521 gagacgtatc acatggtggc gctcgtggcc ggcacgctgg ccggcgtcgc gaccctggcg
     2581 ggtatcgtcc tgctgatcta ccggcggcgc accaccgggc cggtgttcct tgcgaccacc
     2641 cgcaacgaca agctgatgta cgtggtgctg gtggccgcga tcgtgttcgg gatgatcgcc
     2701 actctcaccg gcgaccagac cgctagcggt gaacaccaca actaccgcga gaccgtgtcg
     2761 atctggttcc gctcgctgct ggtcttccag ccgaatgtcg aggccatggc agccgcgacc
     2821 tggcagttca agctgcacat cctggttggc ttcgccctgt tcgcattgat gccgttcagc
     2881 cggcttgtgc acgcgttcac cgtgccgctg cactacctgt tccggccgta catcgtgtac
     2941 cgcagccggg acgcgctcac cccgaccccc tcgcgcggac gcgtccgcgg ctgggacccc
     3001 attggcacac aggatcgaga ccggaagacc cgatgaccca gaccacccac gccgcgcacc
     3061 cccatcacgg cgacgacgta gtcgcgccca tccacgagac gggcaggggc gcgaaccgag
     3121 tcctggccat gtcgaccatc gacttcaccg tgatgttcgc ggtctggctg atgttcggca
     3181 tcctgggcat cgagatccag aaagagctgc tgctcgacga cgtgcagttg tcctggatca
     3241 ccgcgctggc cgtgctgaac ggttcgatgt ggcgcctgcc ggccggcatg atcaccgacc
     3301 ggatcggggg tcgcaaggtc acgatcttca tgctggccgc cactgccgtc cctgcctact
     3361 tcgtggccac agcgcacagc tacggcgttc tgctgatcct ggccttcctg gtcggtttcg
     3421 cggggaacct tttcagcgtc ggcatcgcct ggaacgccgc ctggttcgga cgcgagcgcc
     3481 agggcttcgc gctcggcctg ttcggggcgg gcaacgtcgg cgcctccgtg accaagttca
     3541 tcggcccgcc gctgatcgca ggcacagccg gggccacctt catcttcggc atccagggcg
     3601 gctggcggct ggtgccggtg atctactcgg tgctgctggt ggtgctcgct gtgctcacgt
     3661 ggctgatcgt gccgcgccac gaccggctgg ccggggaaag caagccgatc cgcgacatgc
     3721 tcgctccctt gacccacgta cgggtgtggc ggttcagcct gtactacgtg gcggtcttcg
     3781 gcgcctatgt cgcgttgtcg gcatggctgc cgaagtacta cgtcgacaac ttcaaggttc
     3841 cgctgtacac ggccgccctg ctcaccgcgg tgttcatctt cccggcctcg ctgctgcgtc
     3901 cgctgggcgg ctggatctcc gaccgcatcg gcgcccgcaa ggtgatgtac acaaccttca
     3961 cggtcatgct ggccaccacc ggcatcctga tgatgccgaa cggccacatc gtgatcacgc
     4021 atgccgacgg cagccagacc gagcatctgg cctactcgat caatgtctgg tggttcacct
     4081 tcctggtgtt cctgctcggc tgtgcgatgg gcgtcggcaa ggccggtgtc tacaagcaca
     4141 tccccgaata ctttcccgac aacgtcggct cggtcggcgg cctggtcggc atgctgggag
     4201 ggctcggggg ctttatcctt ccgccgctgt tcgcatacac caagctctgg acgggtttcc
     4261 cgaccagcac cttcttcgtc ctgttcatcc tcaccgcgat ctgcgcgatc tggatgcacc
     4321 tgaccatcgt ccgcatgctg catgcccagg caccgcagct cgccgaccat ttcgacaccg
     4381 agcacattga cacgatttcc ctcaatgagg aggcaaaagc atgagcggtt ccatcgtcga
     4441 gcgactccag ggtggccccg aatggctgaa gtcctgggac ccggagaacg agcagacctg
     4501 ggacaaacgc ctggcctggc agaccctgtg gatcaccacc ttcaccctca cgctctgctt
     4561 cgtcacctgg ttcctgccct cggccatcgt ccccaagctg aacgccatcg gctacagctt
     4621 cacgcgcgag cagctgtatt ggctcgccgc gatcccgggc ctctccggcg gcctgctgcg
     4681 gctggtgtgg atggtgctgc caccggtcat gggcacccgc aagatggtgg ccgcgaccac
     4741 cctgctgctg ttcttcccgg tgctgggctg gggcgtccgc tcgatgtcgc ccaccgcgcc
     4801 ctactgggag ctcctggtgt tggcgttcct ggccggcatc ggtggcggcg cgttctccgg
     4861 tttcatgccg tcgacttcct acttcttccc gcgcaacaaa cagggcaccg cgctcggact
     4921 gcaggccggc atcggcaact tcggggtttc gctggttcag ctcctcaccc cgtggctgat
     4981 cggcct
//
LOCUS       DAOPRQ010000120         4761 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_14508_length_4761_cov_13.792180, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000120 DAOPRQ010000000
VERSION     DAOPRQ010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 4761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4761
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14508_length_4761_cov_13.792180"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..79)
                     /locus_tag="PKJ61_11630"
     tRNA            complement(4..79)
                     /locus_tag="PKJ61_11630"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(44..46),aa:Thr,seq:tgt)
     gene            complement(145..651)
                     /locus_tag="PKJ61_11635"
     CDS             complement(145..651)
                     /locus_tag="PKJ61_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013161859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NACHT domain-containing protein"
                     /protein_id="HOC14513.1"
                     /translation="MTAAVRQLVQRDARPVVLIDGGAGSGKTTLAQKLVAAWSGTTRP
                     QLVSLDDLYPGWGGLAAASAAVPDLITGGGYASWDWARSRPGPWRTLDPERPLIVEGC
                     GAITPASAALSGLTVWVDLDASERRRRAIARDGDSFATHWDEWADQETAHWRSDRPRD
                     LADVILRS"
     gene            complement(668..1852)
                     /gene="manA"
                     /locus_tag="PKJ61_11640"
     CDS             complement(668..1852)
                     /gene="manA"
                     /locus_tag="PKJ61_11640"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HOC14514.1"
                     /translation="MQLLQGTIKHYPWGTTDAIPALLGVPADGRPFAEYWLGAHPLSP
                     STIDGARLDDVIREYPDCLGEVPRQTFGTQLSFLVKILSARHALSVQAHPSRAQAEDG
                     FARENLAGIPLDAPERTYRDSWPKPEVLIALDEFHTLFGFREPQATARLFDALGVAGQ
                     LSSVIGPLTARKGPAALAEVFLDVLSLSGERAQLSEVVCAAAMEHQHRADEVGEFART
                     VLELDEVFPSDPGILAALLLNRVVLQPGEAIYVPAGYMHAHLRGTGVEVMANSDNVIR
                     GGLTTKHLDVSELLTVVDFEAGVPRIINSAPMVDGVASYPTPCPEFEVWRLSPDGRTV
                     DVPGDGSARVVLVISGSLVLSQGGEAATLTHGQAAFLPADRAAATASGTGLAFVTASG
                     VR"
     gene            1983..2279
                     /locus_tag="PKJ61_11645"
     CDS             1983..2279
                     /locus_tag="PKJ61_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017932934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3263 domain-containing protein"
                     /protein_id="HOC14515.1"
                     /translation="MSDPMAARSDLLEASGLSAQQVALLDFEKQWWALPGSKETEIRE
                     RFDISPTRYYQLLNALIDTEAALVHDPLLVKRLRRLRATRQKERTARRLGVNLR"
     gene            complement(2344..2862)
                     /locus_tag="PKJ61_11650"
     CDS             complement(2344..2862)
                     /locus_tag="PKJ61_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020574370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14516.1"
                     /translation="MPRSRPTRPAEPANDPRPTATRHLVRAVGNAVNLSSVLGLGLAL
                     AGRAQLSRGPQALLLAEHYRLPLPKAGAFTIGNVVLVPKRTLAELEQLHPGTAAHEAA
                     HAWQYFGFLGLPFLPAYALASGWSWLRTGDPASANWFERNAGLVSGGYPENPRDNTGF
                     KRIGRVVRPRRP"
     gene            3087..4709
                     /gene="groL"
                     /locus_tag="PKJ61_11655"
     CDS             3087..4709
                     /gene="groL"
                     /locus_tag="PKJ61_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012923547.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOC14517.1"
                     /translation="MAKLIEFNIEARRGLERGMNTLADAVKVTLGPKGRNVVLEKKWG
                     APTITNDGVSIAKEIELEDPYEKIGAELVKEVAKKTDDVAGDGTTTATVIAQAMVREG
                     LRNVTAGANPMSLKKGIEKAVAAIVAELGNQAVDIETREQIAATASISAADTTVGEII
                     AEAMDKVGKEGVITVEESNTFGLDLELTEGMRFDKGFIAPYFVTDAERMEAVLDDPYI
                     LIVNSKVSSLKDLLPVLEKVMQSGKPLLVIAEDVDGEALAGLIVNKIRGTFKSVAVKA
                     PGFGDRRKAMLADIAILTGGTVVSEEVGLKLDAVTVDLLGRARSVRVTKDETTIIDGA
                     GEGEQIAGRVAQIRREIENSDSDYDREKLQERLAKLAGGVAVIKVGAATEVELKERKH
                     RIEDAVRNAKAAVEEGIVPGGGVALLQAAAKVSLPELSGDEATGANIVFVACSAPLKQ
                     IAINAGLEGGVVAEKVSHLEAGNGLNAATGEYVNLIDAGIIDPAKVTRSALQNAASIA
                     ALFLTTEAVIADKPEKAPAMPAGDGGMGGMDF"
BASE COUNT          838 a         1571 c         1658 g          694 t
ORIGIN      
        1 cgctggagcc gcgtgggaga atcgaactcc cgacaaccga tttacaagat cggagctcta
       61 ccaactgagc taacgcggcg cggggtactc cccggcgaac cattgtgccc gacgcggcgg
      121 gccgatgacc gaccgacggc cgattcaact gcgcaagatc acgtcagcga ggtcgcgggg
      181 acggtcggac cgccaatgcg cagtctcctg atcggcccat tcatcccaat gcgtggcaaa
      241 gctgtcgccg tcgcgagcga tcgcacggcg gcgccgctcg ctcgcgtcca agtccaccca
      301 gacggtcagt ccggacagcg ccgcgctggc cggtgtgatc gcgccgcagc cctccacgat
      361 cagcggacgc tcggggtcga gcgtccgcca aggccccgga cgcgagcgcg cccagtccca
      421 ggatgcgtag ccgccgccgg tgatcaggtc cggcaccgcg gcggaagcgg cggccaggcc
      481 gccccagccc gggtagaggt cgtcgaggct gaccagttgc ggacgagtgg tcccggacca
      541 ggcggcgacc aacttctgcg cgagcgtggt cttgcccgag ccggcgccgc cgtcgatcag
      601 caccaccgga cgcgcgtccc gctgcaccag ctggcggacg gccgcggtca cccgcgagtc
      661 gaccacctca gcggacgccg ctggcggtaa cgaaagccaa tccggttccg ctggcggtcg
      721 cggccgcacg gtcggcgggc aggaacgccg cctggccgtg ggtgagcgtg gccgcttcgc
      781 cgccctggct gagcacgagt gacccggaga tgaccagaac cacccgcgct gacccgtccc
      841 cgggaacgtc gaccgtcctg ccgtcggggg agagccgcca gacctcgaac tcggggcacg
      901 gcgtgggata gctcgccacc ccgtccacca tcggtgcgga attgatgatc ctaggaacac
      961 cggcctcgaa gtcgaccacg gttagcagtt cgctcacgtc gaggtgcttc gtcgtcagtc
     1021 cgccgcgaat cacgttgtcg gagttggcca tcacctccac cccggtgccg cgcaggtggg
     1081 cgtgcatgta gccggccggg acgtagatcg cctcacccgg ctgcagtact acccggttca
     1141 gcagcagcgc ggccaggatg ccggggtcgg aggggaagac ctcgtccagt tcgaggaccg
     1201 tgcgggcgaa ctcgcccacc tcgtccgctc ggtgctggtg ttccatagct gcagcgcaga
     1261 ccacctcgga cagctgcgcg cgttcgccgg agaggctgag cacgtccagg aacacctcgg
     1321 ccagcgccgc cggacccttg cgtgccgtga gcggcccgat taccgaagac agctgaccgg
     1381 ccacgccgag cgcgtcgaac agccgggcgg tcgcctgggg ttcgcggaag ccgaagaggg
     1441 tgtggaactc gtccagggcg atcagcacct ccggcttggg ccaggagtcg cgataggtgc
     1501 gctcgggtgc gtccagcggg atgccggcaa ggttctcgcg ggcgaaaccg tcctcggcct
     1561 gtgcgcggga gggatgcgcc tgcaccgaga gggcgtgccg cgccgacagg atcttcacaa
     1621 ggaaggacaa ctgggtgccg aaggtctgcc gcggtacttc gccgaggcag tcggggtact
     1681 cgcggatcac atcgtccagc cgtgcgccgt cgatggtgga cggcgagagg gggtgcgcgc
     1741 ccagccagta ttcagcgaag gggcgcccgt ccgccggaac tccgagcagg gcggggatgg
     1801 cgtcggtggt gccccagggg tagtgcttga tcgtgccctg cagaagctgc atcgagagtc
     1861 ctcgctgtcg ggtaccaggc gcttaggcaa cgttgcgagc cgattgtagc caagggccac
     1921 gggtgccgat cggtgctgac gaacgaatga catcgatgtg gttcagcggc tacgctggcc
     1981 ccatgtccga cccgatggct gcccgttcag acctgctcga ggcgtccggg ttgagcgcgc
     2041 agcaggtggc actgctcgat ttcgagaagc agtggtgggc gctccccggc tcgaaggaga
     2101 ccgagatccg ggagcggttc gacatctcgc cgacccgcta ctaccagctc ctcaacgcgc
     2161 tgatcgacac cgaggccgcc ctagtgcacg atccgcttct ggtgaagcgg ctgcgacgac
     2221 tgcgggccac gcggcagaag gaacgcaccg cccgccgcct cggcgtgaac ctgcgctagt
     2281 cgcgagtggg ccgccccggc ctgtggttaa cgtgaacgaa aaaggaaccg tccacatttt
     2341 tcttcagggg cggcgcggac gcacgacgcg tccgatgcgt ttgaaaccgg tgttgtcgcg
     2401 cggattctcc ggatagccgc ctgagaccag gcccgcgttg cgttcgaacc aattcgcgga
     2461 cgcgggatcg ccggtgcgca accaggacca acccgacgcg agcgcgtagg ccggcaggaa
     2521 gggcaggccg aggaaaccga agtactgcca cgcgtgggcg gcctcgtgcg cggcagttcc
     2581 ggggtggagc tgctcgagct cggccagcgt ccgcttcggc accagcacca cattcccgat
     2641 ggtgaacgcg ccggccttcg gcagcggcag ccggtagtgc tcggccagca gcaatgcctg
     2701 tgggccgcga ctcaactgcg cgcgtccagc gagtgcgagg ccgagcccca gcacggagga
     2761 caggttcacc gcgttcccga cggcgcgcac cagatgccga gttgcggtcg gacgcggatc
     2821 gttggccggt tcggctggac gcgtgggtcg agatctcggc atggggccaa ttgtgcccgt
     2881 cgatgtgtcg gttggggagc cggaattgtt tgcactcgcc tggtgagagt gctaaacatg
     2941 gacttagcac tctcaggttg agagtgccac cagtctgact cgatgagatc gccaagatcg
     3001 tccgtcgcgg gcatcgatgc aagaccaaca cctgagtcca cagtggggca ccgtcccccg
     3061 agatgccaaa ggattgccga aacaagatgg cgaagctgat tgaattcaat atcgaggcgc
     3121 gccgcggcct cgagcggggc atgaacaccc tcgccgatgc ggtcaaggtg accctcggcc
     3181 ccaagggccg caacgtggtc ctggagaaga agtggggcgc ccccaccatc accaacgacg
     3241 gcgtttccat cgccaaggag atcgagctcg aggatcccta cgagaagatc ggcgccgagc
     3301 tggtcaagga agttgccaag aagaccgacg acgtcgctgg cgacggcacc accaccgcca
     3361 ccgtgatcgc ccaggccatg gtgcgtgagg gcctgcggaa cgtcaccgcc ggcgcgaacc
     3421 cgatgagcct gaagaagggc atcgagaagg ccgtcgcggc catcgtcgcc gagctgggca
     3481 accaggccgt cgacatcgag acccgtgagc agatcgccgc gaccgcgtcc atctctgctg
     3541 cggacaccac cgtcggcgag atcatcgccg aggcgatgga caaggtcggc aaggaaggcg
     3601 tgatcaccgt cgaggagtcg aacactttcg gcctcgacct ggagctcacc gaaggcatgc
     3661 gcttcgacaa gggcttcatc gcgccgtact tcgtcaccga cgccgagcgg atggaggccg
     3721 tcctggacga cccgtacatc ctgatcgtca actccaaggt ctcctcgctg aaggacctgc
     3781 tgcccgtgct cgagaaggtc atgcagtcgg gcaagccgct gctggtcatc gctgaggacg
     3841 tggacggcga ggcgctggcc ggtctgatcg tgaacaagat ccgcggcacc ttcaagtcgg
     3901 ttgccgtcaa ggcgccgggc ttcggcgacc gccgcaaggc catgctcgcc gacatcgcca
     3961 tcctcaccgg cggcacggtc gtctccgagg aggtcggcct gaagctggac gcggtgaccg
     4021 tcgacctgct gggccgcgcg cgttcggtgc gggtgaccaa ggacgagacc acgatcatcg
     4081 acggcgcggg cgagggcgag cagatcgccg gacgcgtggc gcagatccgt cgcgagatcg
     4141 aaaactccga ctccgactac gaccgcgaga agctgcagga gcggctggcc aagctggccg
     4201 gcggcgttgc cgtgatcaag gttggcgcgg ccaccgaggt cgagctgaag gagcgcaagc
     4261 accgcatcga ggacgccgtc cgcaacgcga aggctgccgt cgaggagggc atcgtccccg
     4321 gcggtggcgt ggcgctgctg caggccgcag ccaaggtttc cctgccggag ctctccggcg
     4381 acgaggcgac cggcgcgaac atcgtgttcg tcgcctgctc ggctccgctg aagcagatcg
     4441 ccatcaacgc cggccttgag ggtggcgttg tggccgagaa ggtgtcgcac ctcgaggctg
     4501 gcaacggact gaacgcggcc accggcgagt acgtcaacct gatcgacgcc ggcatcatcg
     4561 atccggccaa ggtcacccgt tcggcgctgc agaacgcggc ttcgatcgcg gcgctgttcc
     4621 tgaccaccga agcggtcatc gccgacaagc cggagaaggc tccggccatg ccggccggcg
     4681 acggcggcat gggtggcatg gacttctgat ccagcactga caccacgcga gggcggtccc
     4741 gttaggggcc gccctcgttt g
//
LOCUS       DAOPRQ010000121         4752 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_14548_length_4752_cov_12.315733, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000121 DAOPRQ010000000
VERSION     DAOPRQ010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 4752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4752
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14548_length_4752_cov_12.315733"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1584)
                     /locus_tag="PKJ61_11660"
     CDS             complement(<1..1584)
                     /locus_tag="PKJ61_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016668018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOC14518.1"
                     /translation="MALSGRPATPATEAVRAARDFLFAHAHDYEGAVAGFSWPELAEF
                     NFALEWFDVMAAEHPDRDAVKIVDADLYARSWSYAELSRRSDQVANWLHNLGMRRGDH
                     MIVMLNNTIEIWEILLGLLKVGGVAIPTSTLLSETDLNYRIDRGEARFTIAPDALAAK
                     FASTPAGVIRIGVGGSTPDGWVDFADSHEAALTFTPDGPTPADDTSLLYFTSGTTAYP
                     KLVRHTQVSYPVGHLSTMWWLGVRDGDVHLNISSPGWGKHAWSNFFSPFLAGATVFIF
                     NYDRFDAAALMRIMDTHEVTSFCAPPTVWRMLIQADMTQLRKPPRELVGAGEPLNPEV
                     IAQVRAAWGNTIRDGFGQTEMTCCIGNSPGQLVKDGSMGRPMPGYAVALLDPATGAPA
                     EAEGEICLDLSVPNIALMGGYYHDAEQTAEACRGGFYHTGDIAHVDADGYLTYVGRAD
                     DVFKASDYKISPFELESVLLQHELVAEAAIVPSPDPLRLAVPKAYVCLVSGAVPDETA
                     ARAVFAHARENLAGYQRIRI"
     gene            complement(1872..2861)
                     /locus_tag="PKJ61_11665"
     CDS             complement(1872..2861)
                     /locus_tag="PKJ61_11665"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002516051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HOC14519.1"
                     /translation="MSQTPVKVAVTGAAGQICYSLLFRIASGALLGPDQPVELRLLEI
                     TPALKALEGVVMELDDCAFPLLSKVEIGDDANKVFDGVNLAMLVGARPRTAGMERADL
                     LSANGAIFTAQGKALNASAADDIRVLITGNPANTNALIAMNNAPDIPKERFNALTRLD
                     HNRAITQIAQKAGVPVTAVKHMTIWGNHSASQYPDIFHTEIDGKSAAEVINDQAWVEE
                     YFIPTVAKRGAAIIAARGSSSAASAANATVEHMRDWVKGTPEGDWASMSVPSDGSYGV
                     PEGLISSFPCTVKDGKYEIVQGLELNDFSRGLIDASVAELAEERDAVKELGLI"
     gene            3011..3631
                     /locus_tag="PKJ61_11670"
     CDS             3011..3631
                     /locus_tag="PKJ61_11670"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOC14520.1"
                     /translation="MRLILVRHGRTSSNVGFLLDTGEPGADLDEFGHGQARTLVERLA
                     EHPIEAIYASTLVRTQQTAAPLATALGLDVEVLPGLREISAGDDELAADATRYIGTLM
                     RWHAGEVHARIPGGETAMEFMERFDEAIAVVASRTHDVAMVVSHGAALRVWSSARVRG
                     FVDELGSGHLDNTGVIVVDGSADEGWQLVSLAGVMNWRSADDLVED"
     gene            complement(3655..4503)
                     /gene="purU"
                     /locus_tag="PKJ61_11675"
     CDS             complement(3655..4503)
                     /gene="purU"
                     /locus_tag="PKJ61_11675"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002528634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HOC14521.1"
                     /translation="MDSTHWLLTLTCADRPGIVHRVTGAIVGAHGNITELQQFTSTES
                     GHFFMRVQVQSGFDRSEFEHNFASIAAELDAKWVLDYVGRRRRTLVLASKAGHCLNDL
                     LFRQRSGYLPITMPLIIANHPDLGDLAKFYGVPFEAHQVNAGSKAAVERHILDVVAEH
                     DIELVVLARYMQILSPELCAELAGRAINIHHSFLPGFKGANPYRQAHSRGVKLIGATA
                     HFVTSDLDEGPIIEQNVRRVDHTMDVPQLVAMGQEQESRTLTEAVKLFAEHRVLLDGD
                     RTVIFR"
BASE COUNT          734 a         1509 c         1686 g          823 t
ORIGIN      
        1 gatgcggatg cgctggtacc cggccaggtt ttccctggcg tgggcgaaca ccgcgcgggc
       61 cgcggtctcg tccggcactg ctccggagac caggcagacg taagccttcg gcacggccag
      121 ccgcagcgga tccgggctgg gcacgatcgc ggcctcggcc accagttcgt gctgcagcag
      181 taccgactca agttcgaacg ggctgatctt gtagtcgctg gccttgaaca cgtcgtcggc
      241 gcggccgacg taggtcaggt agccgtccgc gtccacgtgg gcgatgtcgc cggtgtgata
      301 aaaaccgccg cggcacgcct cggcggtctg ctcggcatcg tggtagtagc cgcccatcag
      361 cgcgatgttc ggcaccgaga ggtcgaggca gatctcgccc tcggcttcgg ccggcgctcc
      421 ggtcgccgga tcgagcagcg ccacggcgta gccgggcatc gggcggccca tcgagccatc
      481 tttgaccagc tgaccagggg agttgccgat gcagcaggtc atctcggtct ggccgaaccc
      541 gtcgcgaatg gtgttgcccc aggccgcccg cacctgggcg atcacctccg ggttgagcgg
      601 ttcgccggcc ccgacgagct cccgcggcgg cttgcgcaac tgggtcatgt cggcctggat
      661 cagcattcgc cacaccgtcg gcggggcgca gaagctggtg acctcgtgcg tgtccatgat
      721 ccgcatcagc gcggcggcgt cgaaccggtc gtagttgaag atgaacaccg tcgcgccggc
      781 gaggaacggc gagaagaagt tgctccaggc gtgcttgccc cagccggggg aggagatgtt
      841 caggtgcacg tcgccgtccc ggacgccgag ccaccacatc gtggaaaggt ggcccaccgg
      901 gtaggacacc tgggtgtgcc gcaccagctt cgggtaggcc gtggtgccgg acgtgaagta
      961 cagcagggac gtgtcgtcgg ccggcgtggg cccgtccggg gtgaaagtga gcgcggcctc
     1021 gtgcgagtcg gcgaagtcga cccagccgtc cggagtgctg ccaccgacgc cgatcctgat
     1081 gacgccggca ggtgtggacg cgaacttggc cgcaagtgcg tccggagcaa tggtgaacct
     1141 ggcctcgccg cggtcgatcc ggtagttcag gtccgtctcc gacagcagcg tggacgtcgg
     1201 gatcgccacg ccgcccacct tgagcaggcc gagcaggatc tcccagatct cgatcgtgtt
     1261 gttcagcatc acgatcatgt ggtcgccacg gcgcatgccc aggttgtgca accagttggc
     1321 cacctggtcg gagcggcggg acagctcggc gtagctccac gagcgggcgt agaggtcggc
     1381 gtccacgatc ttcaccgcgt cgcggtcagg atgctctgcg gccatgacgt cgaaccactc
     1441 cagcgcgaag ttgaactcgg ccagttccgg ccagctgaac ccggcgacag ccccctcgta
     1501 gtcgtgggcg tgcgcgaaca ggaagtcgcg ggcggctcgg acggcttcgg ttgccggggt
     1561 cgcgggacgg ccggagagcg ccattggcgt gctgctttcg ggtcggaggt ggggccgtac
     1621 cagggtaacg gcggcaccta cggcgccgta acttagctca gccaccggtc ggtcttggct
     1681 ggctgcccgt cggctggtgc gccgatcgtt ctgtcctctc gcctgtccag agctgtccaa
     1741 gccagcgaaa ctgacacccg ccagggaatg ttcgccggca tctgtccgaa tcgctggctg
     1801 gaagtgccgg acgccggaaa cgcagagtgc cccggtgcgc ggacgctccg gggcactctg
     1861 cggaagctga ctcagatcag gccgagttcc ttcactgcgt cccgctcctc ggcgagctcg
     1921 gcgacggacg cgtcgatcaa gccgcgggag aagtcgttga gctcgaggcc ctggacgatc
     1981 tcgtacttgc catccttcac ggtgcacggg aaggacgaga tcaggccctc gggcacgccg
     2041 tacgagccgt cggacgggac cgacatcgag gcccagtcgc cctcgggggt gcccttaacc
     2101 cagtcgcgca tgtgctcgac ggtggcgttg gctgccgagg cggccgagga cgagcctcgg
     2161 gccgcgatga tggccgcgcc gcgcttggcc acggtcggga tgaagtattc ctcgacccat
     2221 gcctggtcgt tgatgacctc ggcggcgctc ttgccgtcga tctcggtgtg gaagatgtcc
     2281 gggtactggc tggcggagtg attgccccag atggtcatgt gcttgaccgc ggtcaccggg
     2341 acgccggcct tctgtgcgat ctgggtgatc gctcggttgt ggtcgagccg ggtgagggcg
     2401 ttgaaccgct ccttgggaat gtcgggagcg ttgttcatcg cgatcagggc attggtattg
     2461 gccgggttgc cggtgatcag cactcggatg tcgtccgcag cggacgcgtt cagggccttg
     2521 ccctgggcgg tgaagatggc gccgttcgcg ctgagcaggt cggcccgctc catgccggcg
     2581 gtgcgcggcc gggcgccgac cagcatggcc aggttcacgc cgtcgaagac cttgttggca
     2641 tcatcgccga tctcgacctt gctcagcagc gggaacgcgc agtcgtccag ctccatgacg
     2701 acgccctcaa gcgccttgag ggcgggggtg atctccagca gccgcagttc aaccggctgg
     2761 tcggggccga gcagggcgcc gctggcgata cggaaaagca ggctgtagca gatctgtccg
     2821 gcggcgccgg tgaccgcaac cttgacggga gtctggctca cgatgtgctc ctgattggtg
     2881 tggtggaagt gtttgctgac cactgtcgaa actatcagtc tccagccctc cgtttcggcg
     2941 atgggcgcca agactgcgat ggtcgagact gtgcgccggc cgcgtccgcg aggtgcctat
     3001 gctcgtcggt atgcgcttga tcctcgtccg ccacggccgt accagttcca acgtcggttt
     3061 cctgctcgac acgggcgagc ctggcgccga cctggacgag ttcggccacg gccaggcgcg
     3121 gacgctggtt gaacgtctcg ccgagcaccc gatcgaggcc atttacgcgt ccaccctggt
     3181 gcggacgcag cagactgctg cgccgctggc caccgcgctg ggtctcgacg tcgaagtcct
     3241 gcccgggctg cgagagatct cggccggtga cgacgagctg gccgcggacg ccacccgcta
     3301 catcgggacg ctgatgcgct ggcacgccgg tgaggtgcac gcccggattc ccggcggcga
     3361 aaccgcgatg gagttcatgg agcgcttcga cgaggccatc gccgtagtgg cgtcccggac
     3421 gcacgacgtc gcgatggtgg tcagccacgg cgcggctctg cgggtttggt cgtcggctcg
     3481 cgtccgcgga ttcgtggacg aactcgggtc gggacacctg gacaacaccg gtgtgatcgt
     3541 ggtggacggc tccgcggacg agggctggca actggtcagc ctcgccggcg tcatgaactg
     3601 gcggtcggcc gacgacctcg tcgaggactg atctttccgg ctcgccgttt ccgctcagcg
     3661 gaagatgacc gtccgatcgc cgtccagcag aacgcggtgc tcggcgaaca gcttgacggc
     3721 ttcggtcagc gtccggctct cctgctcctg gcccattgcg acgagctggg gcacatccat
     3781 ggtgtggtcc acgcggcgga cgttctgctc gatgatcggg ccctcgtcga ggtcggacgt
     3841 gacgaagtgg gcggtcgcgc cgatcagctt caccccgcgc gaatgcgctt gccggtacgg
     3901 gtttgcgccc ttgaagccgg gcaggaacga gtgatggatg ttgatcgccc gtccggccag
     3961 ctcggcgcac agctcgggcg acaggatctg catgtagcgg gccagcacga ccagttcgat
     4021 gtcgtgctcg gcgaccacgt cgaggatgtg ccgctcgacc gcggccttac tcccggcgtt
     4081 cacctggtgc gcctcgaacg gcaccccgta gaacttggcc agatcgccca gatcagggtg
     4141 gttggcgatg atcagcggca tggtgatcgg caggtagccc gagcgctgcc ggaacagcag
     4201 gtcgttgagg cagtgccccg ccttgctggc cagcaccagg gtgcggcggc ggcgtccgac
     4261 gtagtcaagc acccatttcg cgtccaactc tgcggcgatg ctggcgaagt tgtgttcgaa
     4321 ctcggatcgg tcgaagcccg attgaacctg cacccgcatg aagaagtggc ccgactcggt
     4381 ggacgtgaac tgctgcaact cggtgatgtt gccgtgcgcg ccgacgatcg ctccggtgac
     4441 gcggtgcacg atgccgggac ggtcggcgca ggtgagggtg agcagccagt gggtggagtc
     4501 gatcacggct gaaaggttag cggctggcca acgccggtcc gctcgcgcgt tcgcccaggg
     4561 cgcgggacgc gtccgcgcag tcggctcacc ggaagcccgg ctgaggtatc ttctggaccc
     4621 aggatcagcg atgcagaaag gctcgaggat ggcgaagatc aaggtcgtcg gcacggttgt
     4681 cgaactggac ggggacgaga tgacccggat catctggcag ttcatcaagg accgcctgat
     4741 ccacccctac ct
//
LOCUS       DAOPRQ010000122         4672 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_14857_length_4672_cov_14.418670, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000122 DAOPRQ010000000
VERSION     DAOPRQ010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 4672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4672
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14857_length_4672_cov_14.418670"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="PKJ61_11680"
     CDS             <1..260
                     /locus_tag="PKJ61_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14522.1"
                     /translation="VCPVAPPGAQAYANTLTGYVLWGVLALMVVGVVVSIGAVVGGRV
                     FSMPHASKAGVVGVAVVFVAAVAYMVVPGMLSGITGSGCVG"
     gene            257..1081
                     /locus_tag="PKJ61_11685"
     CDS             257..1081
                     /locus_tag="PKJ61_11685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14523.1"
                     /translation="MSTAAATADQWGRRRLVRTLVVVAMAALLLLGGLVYAVYSAVAS
                     TRTTTAAPAAAAQAAEQLPPGPARRDAIAAAAMLAVPPEASRSGTPSARQSLTLTLPP
                     AGKVGPADVPTGFPKTPEGAIGQLAAIETTVLQGMSIDRAHQVHAAWSTTNAVPVEAW
                     ELTGNIQAFLSSGAGQYADSLHAAVVATPVAAQVKGVDGTDWVLACVLLDVKAQISTQ
                     ARIAYGHCERMQWTDQDGGRWVIGDGPAPARAPSTWPGTDLAGQAGWRTLTSPADQ"
     gene            1098..2558
                     /locus_tag="PKJ61_11690"
     CDS             1098..2558
                     /locus_tag="PKJ61_11690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14524.1"
                     /translation="MNWWDYVNPFTYLGNAAGKIVADGWTAAMLGIWNAGLWLLKLIL
                     GIEDDFLVPDLSENGPMRGIYPYTFWIAGSLILILLMIQLGIAAFRRDGQSLARVLIG
                     TAQFVMVWVAGVVYAVAVVAAAGGLTRALMESLLHVDAMAAWQPWTGFSTADITDGTI
                     ATVLGVMGFFLIFAAFGHLLVMLTRAGALLVLAATNPIAAAGLVWEGGRSWFWKAFRW
                     FHAAAFTPVIMMLMLGLGVQVTSHVALSASATDTLQKAIGTAVPGVMLILVGCFAPLA
                     LFKLLAFVDPGTSSGAAMRAGLAAQGGLQGLLRGGGADESSNAASSSEPSGRSGGEAS
                     TEAATSQRFANSAGGFMGALGVAGQVAATGLGMAVGAGTRAATLGADLTNQMGVGHNT
                     YIPDFSSSRSRGRNNAGPSDQDNPEINGSGPDSDAATPTAAPPAPPTPPMPQAPAAPA
                     ASGAGVAGPSAGAAGAGGAGAAAGGGAAASVPIVPV"
     gene            2574..4085
                     /locus_tag="PKJ61_11695"
     CDS             2574..4085
                     /locus_tag="PKJ61_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HOC14525.1"
                     /translation="MTQVYNQYSKARIGWFPFGLTGWQGAIVAVSTLPVFWSLKEQAW
                     ASAGMFALICAAVSLVTVVPVRGRSAIGWIIASTKFASGGLANWTTFRSRASCGAAED
                     LADVDLPGSLAGIEIHEGPPTGLTGARVAIIQNHATRTWAATAAIVHPGTGMRDADER
                     SRFGRGLADLLDQVSRTELVDEVLFLVRTVPEDGAERDQWIRRHRRLDGPAQVKVIND
                     ELQQVLTGASVRTEAFVMIVVPESRLGKASREAGRGVEARAQILYSQMAEVEAQLKGG
                     MGCTSVSWLTSPELAIACRTGFAPGDRAGIIDALNAQAANPDVNTEIPWAMAGPSGAD
                     AAARHYSHDAWNSISATIKLPVKGAVLGALAPILAPTGTGERRSFLVAFPILSQSKAS
                     RQSASSEWTSEMGQALREKAKMKQSTKARDEADQVRSLEAKLARGSALTRPYAVCTVT
                     APKTVRIAEYGRRLDASIRRAGFAPLRLDLAQDAAFAASVVPLGISLTRKGES"
     gene            4207..>4672
                     /locus_tag="PKJ61_11700"
     CDS             4207..>4672
                     /locus_tag="PKJ61_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOC14526.1"
                     /translation="MSQLLSDFGHELPTTKTAGAPPKEARLFRHVARHGEHRPGRGWS
                     PASAPVTPYRMTSDQAPVFWPFIAGPGLPPTGAQMGVDVLSGSSFYADPIGWVLRDDI
                     PVTNANVGCFGKPGTGKSATTKAFCLRMVDFGYRVLVLGDPKDEYERLCHFFG"
BASE COUNT          727 a         1769 c         1482 g          694 t
ORIGIN      
        1 cggtctgccc ggtggcgccc cccggggcac aggcctacgc gaacaccctg accggctacg
       61 tcctgtgggg cgtcctggcg ctcatggtcg tcggcgtcgt cgtctcgatc ggtgccgtcg
      121 tcggcggccg ggtcttctcc atgccgcacg ccagcaaggc cggcgtggtg ggcgtggcgg
      181 tcgtcttcgt ggcagccgtt gcctacatgg tcgtccccgg catgctgtcc ggcatcaccg
      241 gcagcggctg cgtcggatga gcacggcagc cgccaccgcc gaccagtggg gtcgccgacg
      301 tctcgtgcgc accctggtcg tggttgcgat ggcggctctg ctgctgctcg gtggcctggt
      361 ctatgcggtg tacagcgcgg tcgcctccac ccggaccacg accgccgcac ctgcggctgc
      421 ggctcaagcc gccgagcagc tccctcccgg cccggcgagg cgcgacgcca tcgcggccgc
      481 ggcgatgctg gccgtcccgc ccgaggccag ccgctcgggt accccatcgg ctcgtcaaag
      541 cctcacgctc accctgccgc cagcgggaaa ggttggcccc gcagatgtgc ccaccggctt
      601 ccccaagacc ccagagggag cgatcggaca gctggcagcg atcgagacca ccgtcttgca
      661 gggcatgtcg atcgatcgcg cgcaccaggt gcacgccgcc tggtcgacca cgaacgccgt
      721 ccccgtggaa gcgtgggaac taacgggcaa tattcaggca ttcctgtcca gcggcgccgg
      781 ccagtacgca gacagcctcc acgccgccgt cgtcgccacc ccagtcgcgg ctcaggtcaa
      841 aggcgtcgac ggcaccgatt gggtgctggc ctgcgtcctg ctcgacgtca aggcccagat
      901 ctccacccaa gcccgcatcg cctacggcca ctgcgaacgc atgcagtgga ccgaccagga
      961 cgggggccgg tgggtcatcg gcgacggacc ggcacccgca cgtgccccct ccacctggcc
     1021 cggaaccgac ctcgccgggc aggccggttg gaggaccctg accagcccag ccgaccagta
     1081 gacccaggag agactcgatg aactggtggg actacgtcaa ccccttcacc tacctcggca
     1141 acgctgccgg gaagatcgtc gccgacggct ggactgcggc catgctcggg atctggaacg
     1201 ccggactgtg gctgctcaaa ctcattcttg gcatcgagga cgacttcctc gtccccgatc
     1261 tgtccgagaa cgggcccatg cgaggcatct acccctacac gttctggatc gccggctcac
     1321 tgatcctgat cctgctgatg atccagctcg gcatcgccgc cttccgccgc gacggtcagt
     1381 cccttgctcg agtcctgatc ggtaccgccc agttcgtcat ggtgtgggtc gccggcgtcg
     1441 tctacgccgt ggccgtcgtc gcggccgccg gcggcttgac ccgggccctg atggaatctc
     1501 tcctgcacgt cgacgcgatg gccgcttggc aaccctggac cggcttctcc accgccgaca
     1561 tcaccgacgg caccatcgcg accgtcctgg gagtgatggg cttcttcctc atcttcgccg
     1621 ccttcggcca cctcctggtc atgctcaccc gcgcgggcgc actgctcgtg ctcgctgcga
     1681 ccaacccgat cgccgccgca ggcctggtct gggaaggcgg ccgctcctgg ttctggaagg
     1741 ccttccgctg gttccacgcc gcagccttca ccccggtgat catgatgctg atgctcggcc
     1801 tcggcgtaca ggtcacctca cacgtcgcac tcagcgccag cgccaccgac accctccaga
     1861 aggccatcgg aactgccgtc cccggggtca tgctcatcct cgtcggctgc ttcgcccccc
     1921 tggcgctgtt caagctgctc gccttcgtcg accccggcac ctcatccggg gccgcgatgc
     1981 gcgccgggct ggccgctcag ggcggcctgc aaggtctgct gcgcggcggc ggggccgacg
     2041 agtcctccaa cgcggcctcg agcagcgagc ccagtggccg ctccggcgga gaagcctcca
     2101 ccgaggccgc caccagccaa cggttcgcca atagcgcggg cgggttcatg ggagcactcg
     2161 gggtagccgg ccaggtcgcc gcaaccggcc tcggcatggc cgtcggtgcc ggcacgcgcg
     2221 ccgccaccct cggcgcagac ctgaccaacc agatgggggt cggccacaac acctacatcc
     2281 cagacttctc ctccagccgc tcccgcggcc gcaacaacgc cggccccagc gatcaggaca
     2341 accccgagat caacggctcg ggccccgact cggacgcggc aacccccacc gcagccccac
     2401 cggctccgcc caccccgccc atgcctcaag ccccggccgc gcctgcggcc agcggcgctg
     2461 gagtggcagg tcccagcgct ggcgcagccg gtgccggcgg tgcgggcgct gccgccgggg
     2521 gaggcgcagc cgcgtccgtc cccatcgtcc ccgtctaacg tgaaggaaca gtcatgacac
     2581 aggtctacaa ccagtactca aaggcacgga tcgggtggtt cccgtttggc ctcaccggct
     2641 ggcagggcgc gatcgtcgcg gtctccactc tcccggtgtt ctggtccctg aaggaacaag
     2701 cgtgggcatc ggccggcatg ttcgccctca tctgcgccgc cgtctctctg gtcaccgtgg
     2761 tcccggtccg cgggcggtcc gcgatcggct ggatcatcgc ctccaccaag ttcgcctccg
     2821 gaggactggc gaactggacc accttccggt cccgcgcctc ctgcggtgca gccgaggacc
     2881 tggccgacgt cgacctgccc ggatcccttg ccgggatcga gatccacgag ggtccgccga
     2941 ccggactgac tggcgcacgg gtggccatca tccagaacca cgcgacccgc acttgggccg
     3001 ccactgcagc gatcgtgcac cccggcaccg ggatgagaga cgccgacgaa cgcagccggt
     3061 tcggccgcgg cttggctgac ctgctcgacc aggtcagccg taccgagctc gtcgacgagg
     3121 tcctcttcct ggtccgaacc gtccccgagg acggagccga acgcgaccag tggatccgcc
     3181 gccaccgccg actcgacggg ccggcacagg tcaaggtcat caacgacgag cttcagcagg
     3241 tcctcaccgg cgcgtcggtg cgcaccgaag cattcgtcat gatcgtggtt cccgagtcca
     3301 ggctggggaa ggcctcccga gaagccgggc gaggagtcga ggctcgagcg cagatcctct
     3361 actcccagat ggccgaggtg gaagcccaac tcaaaggcgg catgggctgc accagcgtct
     3421 cttggctgac cagcccggaa ctggccatcg cctgccggac cgggttcgcc ccaggggacc
     3481 gggccgggat catcgacgca ctcaacgccc aagcggccaa ccctgacgtg aacaccgaga
     3541 tcccgtgggc catggccggc ccttcgggcg ctgacgcggc ggcccggcac tacagccatg
     3601 acgcgtggaa ctccatcagc gccacgatca agctgccggt caagggtgcg gtgcttggcg
     3661 cgctggcccc gatcctggcg cccaccggaa ccggggagcg gcgcagcttc ctggtcgcgt
     3721 tcccgatcct ctcgcagagc aaggccagcc ggcagtccgc ctcgagtgaa tggacctccg
     3781 aaatgggtca ggcgctgcgg gagaaggcca agatgaagca gagcaccaag gccagggacg
     3841 aggccgacca ggtccgaagc ctggaggcga agctggcgcg cggctccgcc ctgacccgcc
     3901 cctacgccgt gtgcacggtc accgcgccca agacggtccg gatcgccgaa tacggccgcc
     3961 gcctggacgc ctccatccgc agggccgggt tcgcgccact gcggctcgat ctcgcccagg
     4021 atgccgcctt cgcggcctcg gtcgtcccgc tcggcatcag cctgacccgc aagggagaat
     4081 cctgaccctc ccaccggctc gcgctgcttt aggtggcatg acccacctga gacgacccga
     4141 gcgggacacc acagccgcga tcacggaggt gacccgatga gccgacccag ccgcaccggc
     4201 cgcgacatga gccaactcct gtccgacttc ggtcacgagc tgcccaccac caagacggca
     4261 ggagcacccc caaaagaggc gcgactgttc cgacacgtcg cgcgccacgg agaacacaga
     4321 ccgggccgcg gatggtcccc ggcctcggcg ccggtgaccc cctaccggat gacctccgac
     4381 caagcccccg tgttctggcc gttcatcgcc ggccctggac tgcccccgac tggggcccag
     4441 atgggcgtcg acgtcctctc cgggtcctcg ttctacgccg atcccatcgg ctgggttctg
     4501 cgcgacgaca tccccgtcac caacgccaac gtcggctgct tcggaaaacc aggaacgggc
     4561 aagtcagcca ccacgaaagc gttctgcctg cggatggtgg acttcggcta tcgagttctc
     4621 gtcctcggcg accccaagga cgagtacgag cgcctctgtc acttcttcgg cg
//
LOCUS       DAOPRQ010000123         4667 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_14881_length_4667_cov_13.776453, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000123 DAOPRQ010000000
VERSION     DAOPRQ010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 4667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4667
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14881_length_4667_cov_13.776453"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..865)
                     /locus_tag="PKJ61_11705"
     CDS             complement(<1..865)
                     /locus_tag="PKJ61_11705"
                     /inference="COORDINATES: protein motif:HMM:NF015746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HOC14527.1"
                     /translation="MSETVATASTGNSKWLDWVERVGNKVPNPTIMFVYLIAVVALLS
                     AILSFAGISVTDEVVTPVPKDEFSQINEHLGGTWSIYDSVTGEPAEVPDFIVKEVTFP
                     VRNLLSVEGVRFFFASFVDNFAGFGVVAVVLVSMAGVGVAEHAGLMGALIRKMVKVAP
                     ARWLTFILVFVGVLSSVATDAGYLILVPLAAAAFYSVGRHPLAGLAAAFAGVGAVFGV
                     NLLITPSDSMLTEITNSVLTQLNMAPLEVTQNFFFSIVSSIVLAIVATVVTAKVTMKR
                     LGVYDHADYQGV"
     gene            1150..1968
                     /locus_tag="PKJ61_11710"
     CDS             1150..1968
                     /locus_tag="PKJ61_11710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14528.1"
                     /translation="MRTLKSAQVAAVLLLAGLIATTGCGTLPIPDRSALPSISLPPRP
                     TAWPTLPSRTPAPTATITATETATATATETAPAPEPTPTSASPTQTPTPSPTAGSPSA
                     VPAPPPTQDSGPGWWLLGILIAAVLAVAAGVAGWRHTNAQRQWDRRYQVVRAELEWAD
                     TDLLPQILEYPTAAGAAALWQTGRGRLSAADEELAALGISASRDRRLGQVQTVRRPLA
                     SLLAAMDAETALGADATAEMLRQTRAQVTSARQALRTALNDPSEQAPNDRSDGR"
     gene            complement(2192..2410)
                     /locus_tag="PKJ61_11715"
     CDS             complement(2192..2410)
                     /locus_tag="PKJ61_11715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14529.1"
                     /translation="MPLIRPAKLNGISSCRGDVPIRLATASTTGTKIATTPVELITEP
                     SAATQSISRITSRAPESPAWLASQSPSR"
     gene            2446..3795
                     /locus_tag="PKJ61_11720"
     CDS             2446..3795
                     /locus_tag="PKJ61_11720"
                     /inference="COORDINATES: protein motif:HMM:NF013132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HOC14530.1"
                     /translation="MNAELVRHGLQPFGFFAPTPFGLVVLALGIGYMLLARRWLGDAT
                     PSGGTASRQTIADLLAAYALSGDPRRVVVDADSELGGRTLAELDLARTHGVVVLSVET
                     GPRWHRVLNAADGTTRLTAGDALTVQALAGRSGQLEQLASLGLMEVAATPEDFAQQTR
                     QVGVAELMVPPTSVTDGLKVRDLDLIRRHQLTAIGVRHRGKVVAETPASVRVDSGDTI
                     LVTGEWAAIQRFGRRSDLVLLDLPAEVEETAPAGNQARFALASLLVMVVLMVTGWVPN
                     VLAALIAALLMGATGCIDMPSAYRAIHWPTLLVIVGMLPFAQALERTGGVDLAAQGLL
                     ALVGGAPPGVVLAAIFAATALIGLFVSNTATAVLMAPVAIATATQLGASPYPFAMTVA
                     LAASAAFMTPVSSPVNTLIVEPGRYRFSDFLKLGTPFTILVMIVVAIMVPLVMPLHP"
     gene            3985..4425
                     /locus_tag="PKJ61_11725"
     CDS             3985..4425
                     /locus_tag="PKJ61_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015071038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOC14531.1"
                     /translation="MSIRLENVGIAVRDIEATLAFFTDLGLTVVGRNTVSGEWADTAV
                     GLDGNHAKIAVLQTPDGQGRLEHFEYIHAEAIETDPTLPNEIGMHRVAFAVDDIDKAL
                     EIAARHGCHPLRGVATYADSYKLTYLRGPSGILVMFAQELKKEA"
BASE COUNT          733 a         1557 c         1638 g          739 t
ORIGIN      
        1 ccacgccttg gtagtcggcg tggtcgtaga ccccgagccg cttcatcgtc accttcgcgg
       61 tgaccacggt cgccacgatg gccagcacga tcgaggacac gatcgagaag aagaagttct
      121 gggtgacctc gagcggagcc atgttcagct gggtgagcac cgagttggtg atctcggtca
      181 gcatcgagtc ggacggggtg atcagcaggt tcaccccgaa caccgcgccc acgccggcga
      241 acgccgcagc cagaccggcc agtggatggc gtccgaccga gtagaacgcg gccgccgcga
      301 gtggcaccag gatcaggtag ccggcgtccg tcgccaccga ggacaacacg cccacgaaga
      361 ccaggatgaa ggtcagccag cgtgccgggg cgaccttcac catcttgcgg atcagcgcgc
      421 ccatcagccc ggcatgctcg gccacgccca caccggccat cgagaccagc accaccgcga
      481 ccacgccgaa gccggcgaag ttgtcgacga aggacgcgaa gaagaagcgg acgccctcta
      541 ccgacagcag gttgcgaacc gggaaggtga cctctttgac gatgaagtcc ggcacctcgg
      601 ccggctcgcc ggtcaccgag tcgtagatcg accaggtgcc gccgaggtgt tcgttgatct
      661 ggctgaactc gtccttcggg acgggggtga ccacctcgtc ggtgacgctg atgccggcga
      721 aagacaggat cgcggacagc agggccacca cagcgatcag gtagacgaac atgatcgtcg
      781 gattgggcac cttgttgccc acgcgctcga cccagtcgag ccacttcgaa ttgccggtgc
      841 tggctgtagc cacggtttcg gacatgtcca acctcactca cgctcgcagg caaaccggtt
      901 ggcccgaggc ccacgcttcg cggcacggtt cgcgacgcgc tcggcaggtt cgggcagacc
      961 aacctcaggt tagggtttgg cgagcggtta ggacaccccc agaactggtg gacgggacgg
     1021 ctgaaccggg cggacggaag cgcaggtcgc gcagctcgca cccccgactc tgggactatc
     1081 cgcgcgccgc cgtgctgact agcctgctcg cagcggccga aggccgggat cgaccgatcg
     1141 aggtgatcag tgcgcacact caagtcagct caggtcgcag cagttctgct gctggccgga
     1201 ctcatcgcca cgaccggctg cggcacgcta ccgatccccg atcgttcggc gctgccgtcg
     1261 atctcgctgc cgccccgacc gaccgcatgg ccgacgctgc cgtcccggac gccggcgcca
     1321 acggccacga tcacagctac cgaaacggca acggcgaccg ccactgagac ggcgcccgcg
     1381 ccggagccga cgccaacgtc ggcctcgccg acccagacac caaccccatc accgaccgcc
     1441 ggctcgccgt ccgcagttcc ggcgccacca ccgacgcagg actcgggacc cggctggtgg
     1501 ctgctcggca tcctgatcgc ggccgtgctc gcggtcgcag ccggcgtggc cggctggcgc
     1561 cacaccaacg cccagcggca gtgggaccgg cgctaccagg tcgtccgtgc cgaactggag
     1621 tgggccgaca ccgatctgct gccgcagatc ctggagtacc cgaccgcggc cggagcggca
     1681 gcgctctggc agaccggacg cggccgcctg agcgccgctg acgaggagtt ggccgcgctc
     1741 ggcatcagtg cgtccaggga ccgccggctc ggccaggtgc aaacggtgcg gcgtcccctg
     1801 gcgtccctgc tggcggccat ggacgcggag accgctctcg gcgccgacgc gaccgccgag
     1861 atgctgcggc agacccgggc ccaggtcacg tccgcgcggc aggcgttgcg cacggcgctg
     1921 aacgacccgt ccgagcaggc cccgaacgac cgcagcgacg ggcgatgacc accgacctcg
     1981 gcatggtgct ggcgctgctg gccgctgcga tcgttatgtt cgcgatcggc cggccacgaa
     2041 tggacgtggt cgcgctgctg gcgttggtgg cgttgccgct gaccggagtg ctgaccctgc
     2101 ccgagaccct tgcaggtttc gccgacccca acgtgatcct ggtggccgcc ctgttcgtgg
     2161 tcggcgaagg gctctcccgc accggggtga cctaccggct gggcgactgg ctggccagcc
     2221 atgccggcga ctcgggcgcg cggctggtga tcctgctgat gctctgcgtg gctgcgctgg
     2281 gctcggtgat gagttcgacc ggggtggttg cgatcttcgt cccggtggtg ctggcggtcg
     2341 ccaaacggat cgggacatcg ccgcgacaac tgctgatgcc gttgagtttc gcgggcctga
     2401 tcagcggcat gctcacgctg atcgcgaccg cacccaaccc tggttgtgaa tgccgagctg
     2461 gtccggcacg gcctgcagcc gttcggcttc ttcgcaccga cgccgttcgg gctggtggtg
     2521 ctggcgctcg ggatcggcta catgctgctg gcgcggcgct ggctggggga cgcaaccccg
     2581 tccggcggta ccgcttccag gcagacgatc gccgatctgt tggcggcgta cgcgctttcc
     2641 ggcgaccccc gccgggtagt ggtggacgcg gactccgaac tgggcggacg cacgctggcc
     2701 gagttggatc tggcgcggac gcacggcgtg gtcgtgctga gcgtggagac cggaccgcgc
     2761 tggcaccgcg tcctcaacgc tgcggacggg acgacccggc tgaccgccgg ggatgcgctg
     2821 accgttcagg ctctggccgg tcgatctggg cagctggaac agttggcgtc gcttggcctg
     2881 atggaggtgg cggcgacgcc ggaggacttc gcccagcaga cccggcaggt cggggttgcc
     2941 gagctgatgg tgcccccgac ttcggtgacg gacgggttga aggtgcgcga tctggacctg
     3001 atccggaggc accagctgac cgcgatcgga gtgcggcatc gcggcaaggt ggtcgcggag
     3061 acgccggcga gcgtccgggt ggacagcggc gacacgatcc tggtgaccgg cgaatgggcg
     3121 gcgatccagc gtttcggccg ccgctccgac ctggtgctgc tggacctgcc ggccgaggtg
     3181 gaggagaccg cgccggcggg gaaccaggcg aggttcgcgc tggccagcct gctggtgatg
     3241 gtggtgctga tggtgaccgg atgggtgccg aacgtgctgg cggcgctgat cgcggcgctg
     3301 ctgatgggcg ccaccggttg catcgacatg ccgagcgcct atcgagcaat tcactggccg
     3361 acgctgctgg tgatcgtggg catgctgccg ttcgcacagg cgttggagcg gacgggcggt
     3421 gtcgaccttg ccgcgcaggg gctgctggcg ctggtgggcg gggcgccacc cggggtggtg
     3481 ctggcggcga tcttcgccgc gaccgcgctg atcgggttgt tcgtgtccaa caccgcgaca
     3541 gcggtgctga tggctccggt ggcgatcgcg accgccaccc aactaggggc ttcgccctac
     3601 ccgtttgcga tgaccgtggc gctggctgcg tccgctgcgt tcatgacgcc ggtgtcttcg
     3661 ccggtgaaca cgctgatcgt ggagccgggg cggtaccgct tcagcgattt cctcaagttg
     3721 gggacgccgt tcacgatcct ggtgatgatc gtggtcgcga tcatggtgcc actggtgatg
     3781 ccgctgcacc cgtgaggcgc gaactgtgcg gtcacgcagc cggcagcggt cccgtccatc
     3841 gctaggtgtg gattcgtgca ccgcggacgg tgggctgatc cacactcaag gccggtgcct
     3901 ggccagccaa ctcagagtca ggcctggcga atctcgcctg aagcgtccgc agagcagccg
     3961 agctccactg ggtacattca gggcatgtcc atcagactcg agaatgtcgg aatcgccgtc
     4021 cgcgacatcg aagcgacgct cgcgttcttc accgacctcg gcctcaccgt cgtcggccgg
     4081 aacacggtca gcggcgagtg ggcggatacc gccgtgggtc ttgacggcaa ccatgccaag
     4141 atcgcggtgc ttcagacacc ggacggccag ggtcggcttg agcacttcga gtacatccat
     4201 gccgaggcca tcgagaccga cccgacgctt cccaacgaga tcgggatgca tcgcgtggcc
     4261 ttcgcggtag acgacatcga caaggccctt gagatcgccg cgcggcacgg ctgccacccg
     4321 cttcgtggcg tcgcgaccta tgcggacagc tacaaactca cctacctgcg cgggcccagc
     4381 ggcatcctgg tgatgttcgc ccaggaactc aagaaagaag cctgagcgcg acgcgcttca
     4441 ccggtcggtc gacgcgcgct gcgtcccgtc gatgagatag cgctggctcg tcggggtggg
     4501 agccgaacgg tccccattcg gtcgaaccag gtggcctagc tcactcctgg ccagcgtcgt
     4561 gagttgccgt cgcgtccggc gggaactctc aattcggcgt tgctcgggtc ggcgctttcc
     4621 cattggagcg gatgacggga atcgaacccg cactgtcagc ttgggaa
//
LOCUS       DAOPRQ010000124         4645 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_14970_length_4645_cov_9.232680, whole genome shotgun sequence.
ACCESSION   DAOPRQ010000124 DAOPRQ010000000
VERSION     DAOPRQ010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 4645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4645
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14970_length_4645_cov_9.232680"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..606)
                     /locus_tag="PKJ61_11730"
     CDS             complement(<1..606)
                     /locus_tag="PKJ61_11730"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016667716.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. The beta chain is a
                     regulatory subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HOC14532.1"
                     /translation="MTMTTQTPTQPATGATGRVARVIGPVVDVEFAADEMPDINNKLL
                     IEIETQGRASTIAAEVALHVGDNMVRAISLKPTDGMRRGGKVIDTGEQITVPVGDVTK
                     GRVWNVTGECLNDDLSGVEITERWPIHRDPPKFDALEPKTQMLETGIKVLDLLTPYVQ
                     GGKIGLFGGAGVGKTVLIQEMIYRIAHNFGGTSVFAGVGERT"
     gene            complement(620..1528)
                     /locus_tag="PKJ61_11735"
     CDS             complement(620..1528)
                     /locus_tag="PKJ61_11735"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002526739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HOC14533.1"
                     /translation="MPASLRELRERRKSVSATKKITRAMELVASSRIIRAQQTAARAL
                     PFTRELNRAVSAVAIRSDLDHPLTNEVENPKRVAVLFITSDRGMNGAYSSNVIKTVAA
                     FRQRMEEEGKEVVRYVVGRKGIGYLTYRGMPIEAKWEGFSDSPTFDRAVEISDRLLAD
                     FLLPTEEGGVDEIWAVYTRMESMLTQTPRARRVLPLEVVEGVHEPTKDEAVPLYLFEP
                     DPETVLDSLLPLYIRSRIWFYLLQSAASQLASQQRAMKSATDNAQQLIERLTREANQA
                     RQAEITQEISEIVGGASALADLTEND"
     gene            complement(1533..3176)
                     /gene="atpA"
                     /locus_tag="PKJ61_11740"
     CDS             complement(1533..3176)
                     /gene="atpA"
                     /locus_tag="PKJ61_11740"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013160950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HOC14534.1"
                     /translation="MAELTISPEEIRDALDKFVSNFNPDTASRDEVGTVVTSGDGIAR
                     VEGLPSVMANELLEFANGTTGIALNLDVREIGVVVLGDSEGIEEGSTVKRTGEVLSVP
                     VGDGYLGRVVDAMGNPIDGLGELKDVGERRPLELQAAGVMDRQSVNEPLQTGIKAIDA
                     MTPIGRGQRQLIIGDRKTGKTAIAVDTIINQKAAWATGDPKQQVRCIYVAIGQKGSTI
                     AALRTVLEESGAMEYTTIVHSPASDPAGYKYIAPYSGSAIGQHWMYQGKHVLIIFDDL
                     TKQAEAYRAMSLLLRRPPGREAYPGDVFYLHSRLLERCAKLSDDLGGGSMTGLPIIET
                     KANDVSAYIPTNVISITDGQIFLQSDLFNANQRPAIDVGISVSRVGGAAQVKAMKKVA
                     GTLKIGLAQYRDMEAFAMFASDLDAASRRQLDRGARLVELLRQPQYSPYPVAEQVISV
                     WAGTTGEFDSVPVNELLGFEREFLDHLRHNTKVLETIAQTGDFDADTQQATKDALAQF
                     RTQWVSGGGESLVSGDQAAEAMADEDMDHEQIVVNRKRS"
     gene            complement(3202..3999)
                     /locus_tag="PKJ61_11745"
     CDS             complement(3202..3999)
                     /locus_tag="PKJ61_11745"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009967.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HOC14535.1"
                     /translation="MNAAAEARLSELDRVLDARELTADLAAELFAVADLLTGQTQLRN
                     AVADPTSSADQRRELASAVLGSRVSADAAAVAGEAAALKWGSPANLVAALERQGVRTL
                     FGTAQVAGRLDSVEEELFRFARSVAGSPELRAALDDRNAPVPARQQLIGDLLADRAKP
                     ETVALAQRAAAVQTRSFEAVLDEYLKLAAAAQQRALAVVTVAVPLSAAQKARLMAALS
                     RQLGRPVSLQVLIDPAVLGGARVRVGDEVIEGTVAGRLAAAEHELTK"
     gene            complement(3996..4544)
                     /locus_tag="PKJ61_11750"
     CDS             complement(3996..4544)
                     /locus_tag="PKJ61_11750"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020575219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HOC14536.1"
                     /translation="MPLAGEAESPFGPLLPEHPIELWVGIGLALIITLVVWKLVVPVF
                     EKTYAERTAEITGGIEKAEEAQRQAADALAEYTAQLATARTEASQIREQAKAQAAVIA
                     AEIKEQAQAESARMIASAKAQIDAERAQVVHQLRAEVGGLATQLAGRIVGESLADDER
                     AKRSVDRFIAELEAADTVGEKA"
BASE COUNT          651 a         1621 c         1549 g          824 t
ORIGIN      
        1 ggtgcgctcc cccacgccgg cgaacaccga ggtgccaccg aagttgtggg cgatccggta
       61 gatcatctcc tggatcagca cggtcttccc cacaccggcg ccgccgaaca ggccgatctt
      121 gccgccctgg acgtacgggg tgagcaggtc gagcaccttg atcccggtct cgagcatctg
      181 ggtcttcggc tccagcgcgt cgaacttcgg cggatcgcgg tggatcggcc agcgctcggt
      241 gatctccacc cccgacaggt cgtcgttcag gcactcgccg gtcacgttcc agacccggcc
      301 cttggtgacg tcgccgaccg ggacggtgat ctgctcgccg gtgtcgatca ccttgccgcc
      361 gcggcgcatg ccgtcggtcg gcttgagcga gatcgcgcgc accatgttgt cgccgacgtg
      421 cagcgccacc tcggctgcga tcgtgctggc ccgtccctgg gtctcgattt cgatcagcag
      481 cttgttgttg atgtcgggca tctcgtcagc ggcgaactca acgtccacca cggggccgat
      541 cacccgggcc acgcgcccgg tggcgccggt ggcgggctgg gtcggggtct gcgtcgtcat
      601 ggtcatctgt gtgcctcgcc tagtcgttct cggtcaggtc ggccagcgca ctggcaccgc
      661 cgacgatttc gctgatttcc tgggtgatct cggcctgccg ggcctggttg gcttcccggg
      721 tgagccgctc gatcagttgc tgcgcgttgt cggtggccga cttcatggcc cgctgctggc
      781 tggccagctg ggacgccgcc gactgcagca ggtagaacca gatgcggctg cggatgtaca
      841 gcggcagcag gctgtccagc acggtctccg gatcgggctc gaacaggtag agcggtaccg
      901 cctcgtcctt ggtcggttcg tggacaccct ctaccacttc cagcggcagg acgcggcgcg
      961 ctcgcggcgt ctgggtgagc atcgactcca tccgggtgta gaccgcccag atctcgtcca
     1021 cgcccccttc ttcggtggga agcaggaagt cggccagcag ccggtcggag atttccaccg
     1081 cccggtcgaa ggtcggcgag tcggagaagc cctcccactt ggcctcaatc ggcatgccgc
     1141 ggtaggtcag gtagccgatg cccttgcggc ccacgacgta gcggacgacc tccttgccct
     1201 cctcctccat gcgctggcgg aaggcggcca cggtcttgat cacgttcgag gaataggcgc
     1261 cgttcatgcc ccgatcggac gtgatgaaca gcaccgctac ccgcttgggg ttctccacct
     1321 cgttggtgag gggatggtcg aggtctgacc ggatcgcgac cgccgagacg gcacggttca
     1381 gctcccgggt gaacggcagc gcccgggccg cggtctgctg cgcccggatg atccgtgagg
     1441 acgcgaccag ctccatcgcc ctggtgatct tcttggtcgc agacaccgac tttcggcgtt
     1501 cccgcagctc ccgcaggctg gctggcattc gatcagctcc gcttccggtt caccacgatc
     1561 tgttcgtggt ccatgtcctc gtcggccatc gcctccgctg cctgatcgcc gctgaccaac
     1621 gactcgccgc caccgctgac ccactgggtc cggaactgcg caagcgcgtc cttggtggcc
     1681 tgctgggtgt cggcgtcgaa gtcaccggtc tgtgcgatcg tctccagcac cttggtgttg
     1741 tgccgcaggt ggtccaggaa ctcccgctcg aagccgagca gctcgttcac cggcacgctg
     1801 tcgaactcac cggtggtgcc agcccacacg ctgatcacct gctcggccac cgggtacggg
     1861 ctgtactgcg gctggcgaag cagctccacc aagcgtgcgc cacgatcgag ctggcgccgc
     1921 gaggccgcgt ccaggtcgga cgcgaacatc gcgaacgcct ccatgtcgcg gtactgcgcc
     1981 aggccgatct tgagcgtgcc ggccaccttc ttcatcgcct tcacctgcgc ggcgccgccc
     2041 acgcgggaca ccgagatgcc cacgtcgatg gccggacgct ggttcgcgtt gaacaggtcc
     2101 gactgcagga agatctggcc gtcggtgatt gagatcacgt tggtcgggat gtatgccgag
     2161 acgtcgttgg ccttggtctc gatgatcggc aggccggtca tcgagccgcc gccgagatcg
     2221 tccgacagct tcgcgcagcg ctcgagcagg cggctgtgca ggtagaagac gtcgccgggg
     2281 taggcctcgc ggcccggcgg ccggcgcagc agcagagaca tcgcccggta ggcctcggcc
     2341 tgcttggtga ggtcgtcgaa gatgatcagc acgtgcttgc cctggtacat ccagtgctgg
     2401 ccgatggccg agcctgagta gggcgcgatg tacttgtagc ccgccgggtc ggacgcgggc
     2461 gagtgcacga tcgtggtgta ctccatcgcg ccggactctt ccaggaccgt acgcagcgcc
     2521 gcgatcgtgg agcccttctg gccgatcgcg acgtagatgc accgcacctg ctgcttgggg
     2581 tcgccggtgg cccaggccgc cttctggttg atgatcgtgt cgaccgcgat cgccgtcttg
     2641 ccggtcttgc ggtcgccgat gatcagctgg cgctggccgc ggccgatcgg ggtcatcgcg
     2701 tcgatggcct tgatgccggt ctgcagcggc tcgttgacgc tctggcggtc catcacgccg
     2761 gcagcctgca gctccagcgg acgccgctcg ccgacgtcct tcagctcacc gagaccgtcg
     2821 atcgggttgc ccatcgcgtc cacgacccgg ccgaggtagc catcgcccac cgggacgctg
     2881 agcacctcgc cggtgcgctt gaccgtcgag ccctcctcga tgccctcgga gtcgcccagg
     2941 acgacgacgc cgatctcgcg gacatcgagg ttcagcgcga tgccggtggt gccgttggcg
     3001 aactcgagca gctcgttggc catgaccgaa ggcaggccct cgacccgggc aatgccgtca
     3061 ccggaggtga ccacggtgcc aacttcgtcc cgggaagcgg tgtcggggtt gaagttggac
     3121 acgaacttgt ccagcgcgtc ccggatctct tcggggctga tggtcagttc cgccatttgt
     3181 gcaccttctg ctttcgattc tctacttggt cagttcatgt tcggcggcag cgagccgacc
     3241 ggcgacggtg ccctcgatca cctcgtcacc gacccgcacc cgcgcaccgc cgaggacggc
     3301 cgggtcgatc agcacctgca ggctcaccgg gcggcccaac tggcggctca acgcggccat
     3361 cagccgggct ttctgcgctg cactcaacgg cacggccacg gtcaccacgg ccagcgcccg
     3421 ttgctgggct gccgcagcga gcttgaggta ttcgtcgagg acggcttcga aggaccgggt
     3481 ctggacggcg gccgcgcgct gtgccagtgc caccgtctcc ggtttggccc ggtcagccaa
     3541 caggtcgccg atcagctgct gccgcgcggg taccggtgcg ttgcggtcgt ccagcgcggc
     3601 cctcagttcg ggactgcccg ccaccgaccg agcgaaccgg aacaactcct cctcaacgct
     3661 gtccagccgg ccagccacct gcgcggtgcc gaacagtgtc cggacgccct gacgctccag
     3721 cgcggcaacc aggttggccg ggctgcccca tttcagtgcg gccgcctcac cggcgaccgc
     3781 ggccgcgtcc gccgaaaccc ggctgccgag cacagccgac gcgagttcgc gccgctggtc
     3841 ggctgaggat gtgggatcgg ccaccgcgtt gcggagctgg gtctgcccag tcagcaggtc
     3901 ggcgacggcg aacaactccg ccgccaggtc ggcagtgagc tcgcgggcgt caaggacccg
     3961 atcgagctcg gaaagccggg cctcggcggc ggcgttcacg ccttctctcc cacggtgtcg
     4021 gcagcttcga gctcggcgat gaagcggtcg acgctccgct tggcccgctc gtcgtcggcc
     4081 agcgactcgc cgacgatccg tccggccagc tgggtggcca gcccacccac ctcggcgcgc
     4141 agctgatgca cgacctgagc ccgctcagcg tcgatctggg ccttggcgga cgcgatcatt
     4201 cgcgccgatt cggcctgcgc ctgctccttg atctctgcgg ctatcaccgc ggcctgcgcc
     4261 ttggcctgct cgcggatctg gctggcctcg gtgcgtgccg tagccagctg ggcggtgtac
     4321 tcggcgaggg cgtccgcggc ttgccgctgg gcctcctcag ccttctcgat cccaccggtg
     4381 atctcggccg tgcgctcggc gtacgtcttc tcgaaaaccg gcacgaccag cttccacacc
     4441 acgagggtga tgatgagcgc gaggccgatg ccgacccaga gctcgatcgg gtgctcggga
     4501 agcagcggcc cgaatgggct ctctgcctcg cctgcgagtg gcagcacggc tcagctcact
     4561 gaatggcgaa ggcgagggcg atgccgatga tggccagcgc ctcgaccacg gcgaagccga
     4621 tccacgcggt gcccatcatg gcgcc
//
LOCUS       DAOPRQ010000125         4563 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_15326_length_4563_cov_10.356921, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000125 DAOPRQ010000000
VERSION     DAOPRQ010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 4563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4563
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15326_length_4563_cov_10.356921"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="PKJ61_11755"
     CDS             <1..245
                     /locus_tag="PKJ61_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HOC14537.1"
                     /translation="LAEVFGGTAPAPGGQFRAAEFAETEWGPVPADASVWAGVRLERT
                     AETGWSTLTTCVLEHLVVEGDGEPLVHYRGRYPRLG"
     gene            complement(279..1205)
                     /locus_tag="PKJ61_11760"
     CDS             complement(279..1205)
                     /locus_tag="PKJ61_11760"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015583605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HOC14538.1"
                     /translation="MNTAVTVVGEALIDIVVPVSGEPAEHVGGSPANVAIGLSRLGHN
                     TRLATHIGTDARGQRIAALFAAEDVELTPGSTDADRTPTATANLDAAGVATYDFDLEW
                     TLDPATVDLSSGGHLHTGSIAATLPPGGDAVHEIVQRAREHATVSYDPNARPTLMGSP
                     EEARPIIERLIRLADVVKASEEDVAWLYGGAPVTQVLAEWAAMGPAVCAVTQGSEEVV
                     ILAAGELQRFPTLPTKVADTVGAGDSFMAGLISGLLDAGLLGDAEARERLRFAEWPVL
                     EIAVSRALACAAITVSRAGANPPTRAELEVEV"
     gene            complement(1216..2139)
                     /locus_tag="PKJ61_11765"
     CDS             complement(1216..2139)
                     /locus_tag="PKJ61_11765"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOC14539.1"
                     /translation="MPVATRRPVPDRLPPLQLPDLAGRRIVITGATSGIGLESAIALA
                     QARAALVLGVRDVAKGEAAADRIRERVPSARVAVEQIELGSLASVADFALRVGSSPVD
                     VLISNAGLSTADASARTEDGFDLQIGVNYLGSFALVAGLWPALVAAQNPRVVTLGSLM
                     AHRGRIDPDFGRPRKSTYRSYSDSKLGQVVLAMELRRRSRASGTGVEAVAAHPGWSQT
                     AIFDTAGPPAVVETIGNFIGALQSPADGAQPVLLAATAPRPSMYYGPTRWFGAAGPAG
                     PSALPKGARTAGAAERLWRLSTELTGVEFEP"
     gene            complement(2139..2441)
                     /locus_tag="PKJ61_11770"
     CDS             complement(2139..2441)
                     /locus_tag="PKJ61_11770"
                     /inference="COORDINATES: protein motif:HMM:NF021378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HOC14540.1"
                     /translation="MARLLRTAAHMAVATSVHGKVQRRQSARWAAADQAQAQAAPAAP
                     APAPAPVAPSAPAPAPAGDDAMGRKLAQLTQLGELKAAGILSEAEFEAKKALILAG"
     gene            complement(2454..2906)
                     /locus_tag="PKJ61_11775"
     CDS             complement(2454..2906)
                     /locus_tag="PKJ61_11775"
                     /inference="COORDINATES: protein motif:HMM:NF039728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6325 family protein"
                     /protein_id="HOC14541.1"
                     /translation="MSAAQDWITDHIGPVEVVVFTLPATGSSSAWNSLLAAVDAHSVR
                     LLDLEFVRRISADEVEILSAEDLPDLVPVTELSGATSELVGEEDALELLGELADGEVA
                     AVVLVEHIALLPALQDFEDAGSRLLLTGSVELAALEEALDEPVTSEND"
     gene            complement(2983..>4563)
                     /locus_tag="PKJ61_11780"
     CDS             complement(2983..>4563)
                     /locus_tag="PKJ61_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004809060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HOC14542.1"
                     /translation="EPNGYLHIGHAKAIAVDFGTAEEFGGICNLRLDDTNPEVEETEY
                     VDSIVADIEWLGFRPYQLLHASDYFGQLYEWAEHLIEQGLAYVDDQDNEAISAQRGGF
                     GKPGVESPFRDRSIADNLDLFRRMKAGEFPDGDRVLRAKIDMNHENMALRDPVMYRIR
                     HSHHFRTGDDWCIYPTYDWAHGQSDAIEGVTHSICTLEFDTHRPLYDWYLEHLPLPAE
                     KPKQIEFARLELTHTVTSKRRLRKLVEDGTVDGWDDPRMPTLRGLRRRGYPARAIRAF
                     CTEVGTTRTNSRQSIEALEAYVRRELNATAQRRMAVIRPLRVVIDNWPADAAGAPVTE
                     YFEVANNPENAADGTRQVAFSGELFIEQDDFAEVPPPKFFRLTIGREVRLRGAYLVTA
                     TSIEKDAAGEVVCVHATYDPATRGGDAPDGRKVKSTMHWVATDHAVDITVALYERLFT
                     THVPGEATGEPLDDLNPNSRELVAGKAEAALGEAAPGVVVQFERLGYFAHDPEQPGLF
                     HRTVGLRDEWAAIQKRQG"
BASE COUNT          650 a         1519 c         1672 g          722 t
ORIGIN      
        1 ggctggccga ggtgttcggc ggcactgcgc ccgcgccggg cggacagttc cgcgcggcgg
       61 agtttgccga gaccgaatgg ggaccggtgc ccgcggacgc gtccgtttgg gctggggtgc
      121 gtctggagcg gacggcggag acgggctggt cgacgctgac cacctgcgtg ctggaacacc
      181 tggtcgttga aggagacggt gagccgctgg tgcactaccg cggacggtat ccgcggctgg
      241 gctgaagtga ccgtggcgcc gaagcgagcg acctggacct agacctcgac ctcgagttcg
      301 gcgcgggtcg gcgggttggc gcccgccctg gagactgtga tcgcagcgca cgcgagggcg
      361 cggctgaccg cgatttccag caccggccat tcggcgaagc gcagccgttc acgcgcttcg
      421 gcgtcgccca gaaggcccgc gtccaggaga cctgagatca ggccggccat gaacgagtca
      481 ccggcgccga ccgtgtctgc gaccttggtc ggcagggtgg ggaagcgctg caactcgccg
      541 gcggcgagga tcaccacttc ttcgctaccc tgagtgaccg cgcaaacggc cggccccatc
      601 gcggcccatt cggccaacac ctgcgtgacc ggcgcaccgc cgtagagcca ggccacgtcc
      661 tcctcggatg ctttgaccac atcggccagg cgaatcagcc gctcgatgat cggacgggcc
      721 tcctcggggg agcccatcag ggtgggccgt gcgttcgggt cataggagac ggtggcgtgc
      781 tcgcgggcgc gctgcacgat ctcatggacg gcgtcgcctc cggggggcag tgtcgccgcg
      841 atcgagccgg tgtgcaggtg gccgccgctg ctgagatcca ccgtcgccgg gtcgagcgtc
      901 cactccaggt cgaagtcgta ggtggccacg ccggccgcgt cgaggttggc ggtggcggtc
      961 ggcgtccggt cggcgtcggt actgcccggg gtgagctcga cgtcctcggc ggcgaagagc
     1021 gcggcgatgc gctggccacg cgcgtcggtg ccgatatggg tcgcgagccg ggtgttgtgc
     1081 ccgagccggg acaagccgat ggcgacgttg gcggggctgc caccgacatg ttcggcgggt
     1141 tctccggata cgggcacgac gatgtcgatc agagcttcgc cgaccacggt gactgcggtg
     1201 ttcataccgg ataccttagg gctcgaactc gaccccggtg agctccgtgg acagccgcca
     1261 gaggcgttca gcggcccccg cagtccgggc acccttcggc agtgcggacg ggccggcggg
     1321 tccggctgca ccgaaccagc gggtggggcc gtagtacatc gaaggacgcg gtgcggttgc
     1381 ggccagcagg acgggctggg ccccgtccgc cggtgattgc agggcaccga tgaagttgcc
     1441 gatggtctcg acgacagcgg gcggcccggc ggtgtcgaag atggcggtct gcgaccagcc
     1501 ggggtgcgcg gccacggcct ccacgcccgt cccggacgcg cggctgcggc gtcgcaattc
     1561 catcgcgagc accacctggc cgagcttgga gtcggagtag gaccgatagg tggacttgcg
     1621 gggccggccg aagtccgggt cgatgcggcc ccggtgggcc atcagagagc ccagcgtgac
     1681 cacccgcggg ttctgggcgg cgaccagagc cggccacagg ccggcgacca gtgcgaacga
     1741 accgaggtag ttgaccccga tttgcaggtc gaagccgtcc tcggtgcgcg cggacgcgtc
     1801 cgctgtgctc aagccggcgt tactgatcag cacgtcgacc ggtgagctgc ccacccgcag
     1861 ggcgaagtcg gcgacgctgg ccagcgagcc gagttcgatc tgctccaccg ccactctcgc
     1921 ggacgggacc cgctcgcgaa tccggtcggc cgcagcctcg cctttcgcga catcccgcac
     1981 gcccagaacc agcgcggcgc gggcctgggc cagagcgatc gcggactcca gcccgatacc
     2041 gctggtggca ccggtgatca cgatccggcg tccggcgagg tcgggcagtt gcagcggcgg
     2101 cagccggtcg gggaccggcc gccgcgtggc taccggcatc aaccggccag gatcaaggcc
     2161 ttcttggctt cgaactcggc ttcggagaga atgccggccg ccttcagctc cccgagttgg
     2221 gtgagctggg ccagcttgcg gcccatcgcg tcgtcgccgg ccggagctgg cgcaggtgcg
     2281 gacggcgcca ccggtgccgg ggccggcgca ggcgcggcag gtgcggcttg cgcctgggcc
     2341 tggtcggcgg cagcccagcg ggcggactgg cgacgctgca ccttgccatg gacagaggtt
     2401 gccacggcca tgtgggcggc ggtacggaga aggcgtgcca tcgggattcc tattcagtcg
     2461 ttctcggagg tgacgggttc gtccagcgct tcttccagcg cggccaactc gaccgaaccg
     2521 gtcagcagca agcgggagcc ggcgtcctcg aagtcctgca gcgcgggcag cagggcgatg
     2581 tgctctacca ggacaacagc ggcaacctcg ccgtcggcga gctctccgag cagttcgagg
     2641 gcgtcctctt cgccgaccag ttcgctggtc gcgccgctca gttcggtgac cggcaccagg
     2701 tcgggcaggt cctcggcgct gaggatctcg acctcgtcgg cgctgatcct gcggacgaac
     2761 tccaggtcga gaaggcggac gctgtgcgcg tccaccgcgg ccagcaggct gttccaggcg
     2821 gacgagctgc cggtggccgg cagggtgaag acgacgactt ccacgggacc gatgtggtcg
     2881 gtgatccaat cttgggctgc gctcatgtcg ctcagcctaa cgacgtgcac cagcccgcgg
     2941 gcgcacagcg gtgttcggtc tcggtggcgc gccgataggg gctcagcctt ggcgcttctg
     3001 gatcgccgcc cactcgtccc gcaacccgac cgtccgatgg aacaacccgg gctgctccgg
     3061 gtcgtgggcg aagtagccga gccgttcgaa ctgcaccacc acaccgggtg cggcttcgcc
     3121 aagggccgcc tcggccttgc ccgccaccag ctcgcgggag ttggggttca ggtcgtccag
     3181 gggctcgccg gtggcctcgc cgggcacgtg ggtcgtgaac aaccgctcgt agagcgcgac
     3241 cgtgatgtcg acggcatgat cggtcgccac ccagtgcatc gtggacttaa ccttgcggcc
     3301 gtccggtgcg tctccgccac gcgtggccgg gtcgtaggtc gcgtggacgc agaccacctc
     3361 gccggcggcg tccttctcga tcgaggtggc ggtcaccagg taggcgccgc gcagccgcac
     3421 ctcgcgtccg atggtcagcc ggaagaactt gggcgggggt acttcggcga agtcgtcctg
     3481 ctcgatgaac agctcgccgc tgaaggcgac ctggcgagtg ccgtccgccg cgttctccgg
     3541 gttgttcgcc acctcgaagt actcggtcac cggcgcgccc gcggcgtccg ccggccagtt
     3601 gtcgatcacc actcgaagcg gacggatcac cgccatccgg cgctgggccg tggcgttcag
     3661 ctcgcgccgc acgtaggcct ccagcgcctc gatggactga cggctgttgg ttcgggtggt
     3721 gccgacctcg gtgcagaacg cgcggatcgc gcgagccggg tagccccgtc gccgcaggcc
     3781 gcgcagggta ggcatccggg ggtcgtccca gccgtcaacc gtgccgtcct cgaccagctt
     3841 ccgcagcctg cgcttgctgg tcacggtgtg ggttagctcc agccgggcga actcgatctg
     3901 cttgggcttc tccgccggca gcggcaggtg ttcgaggtac cagtcgtaca gcggacggtg
     3961 ggtgtcgaac tccagcgtgc agatcgaatg ggtgacgccc tcgatcgcgt ccgactggcc
     4021 gtgcgcccag tcgtaggttg ggtagatgca ccagtcgtcg ccggtgcgga aatggtggct
     4081 gtgccggatc cggtacatca ccgggtcgcg cagcgccatg ttctcgtgat tcatgtcgat
     4141 cttggcgcgc agcacgcggt cgccatcggg gaactccccg gccttcatcc gccggaagag
     4201 gtcgaggttg tcggcgatcg agcggtcccg gaacgggctc tcgacgccgg gcttgccgaa
     4261 gccgccgcgc tgggccgaga tggcctcgtt gtcctggtcg tccacgtagg ccaggccctg
     4321 ctcgatcagg tgttcggccc actcgtagag ctgcccgaag tagtcggacg cgtgcagcag
     4381 ctggtagggc cggaaaccca gccactcgat gtcggcgacg atcgaatcga cgtactcggt
     4441 ctcctcgacc tcgggattgg tgtcgtcgag gcggagattg cagatgccgc cgaactcctc
     4501 ggccgtgccg aagtcgaccg cgatcgcctt ggcgtgaccg atgtgcaggt agccgttcgg
     4561 ctc
//
LOCUS       DAOPRQ010000126         4481 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_15687_length_4481_cov_11.856304, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000126 DAOPRQ010000000
VERSION     DAOPRQ010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 4481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4481
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15687_length_4481_cov_11.856304"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..883
                     /locus_tag="PKJ61_11785"
     CDS             <1..883
                     /locus_tag="PKJ61_11785"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOC14543.1"
                     /translation="TILRDGELVGTHPAAELSIHDIIQLMVGRKIAVDIRPEPRASTT
                     PVLEVEGLCSATLLKDVSFTLEKGEILGFAGLMGAGRTETARALIGADAKTSGTVKID
                     GKLANITHPGDAVRKGIGYLSEDRKRYGLILQQTVAANTVLASISKYTRYGLLQDAEM
                     RKAGEKWVNVLATKTPSVRQLVRNLSGGSQQKVVLAKWLEQDCDILIFDEPTRGIDVG
                     AKQEIYTLLDQLTKEEGKSIIMISSEMEEVLRMSDRIVVMCDGRITGVLPNAEATQEK
                     IMELATRFNPTEHQIEA"
     gene            888..1895
                     /locus_tag="PKJ61_11790"
     CDS             888..1895
                     /locus_tag="PKJ61_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOC14544.1"
                     /translation="MSTNTTAVSASTMKWAIPERYKVFLQQGMVLLVVVLLAVFFTIM
                     KPNFASWSNISSLLLASVVTGVQALGLSFVIATGGIDLTPGLGMAATSTFTAILIADA
                     GLRLPIWVGILGGLLAGALIGLINGSLVAYVRMQPMIATLAMMMVCAGTALVIANGKT
                     ISLRSIQGWESIAMGQVIPGVPNGVTILILLAIIAGVVMNKTAIGRYSLAIGSNKEAT
                     RISGVNTRRWEVLAYVICGVFTGISGILMSSRLAAAQPTLGQGYEMYAIAAAVIGGTS
                     LRGGRASIFGAVIGAVTIQTIFNGLTVMGVQDQWQKVVLGVVVLIAVFVDIMRRGGKE
                     E"
     gene            1975..2964
                     /locus_tag="PKJ61_11795"
     CDS             1975..2964
                     /locus_tag="PKJ61_11795"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOC14545.1"
                     /translation="MKKFIAGVAGATIAVLALSACSAGGTAATSAAAGASSAAGGSIK
                     VSLIAKGTSDYWTLVKNGAEAAGKDLGVEVTYNATDTENEGDKQLNLLTAAINDKPQG
                     VAIAPQDGAQDGAPTVLDQAKAANIPVAVFDTPLKSWDGAIATIASDNEGIGAQAAEN
                     LSKLIGGKGKVAMVIQGVTGTAALRRDGFANWMKANAPDIQVVDIQNGEADPAKSRDK
                     AQAILQANPDLAGFAGTSDYSTIAIADEVAAKGGTVKVVGVDASPDVLTLLSEGKIDG
                     IVTQNPYQIGYKTVSTLVDQVKGKAPAEKTIVSEAAWVTKDNMNNPEIKQITG"
     gene            2989..3405
                     /locus_tag="PKJ61_11800"
     CDS             2989..3405
                     /locus_tag="PKJ61_11800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14546.1"
                     /translation="MPTQPPPIAEISIRHWLVVIGAGLLMSLNVLVFLAAGILLPPLA
                     ESLGVGLGQVMVFVSINMVAGALVLAVAGPFLIRRFGSRWLSLLGGLVTGASIFAVSL
                     VTELWQLIAALIGFALVTGPRSWRPTGAGRRVGGAA"
     gene            3807..>4481
                     /locus_tag="PKJ61_11805"
     CDS             3807..>4481
                     /locus_tag="PKJ61_11805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14547.1"
                     /translation="MASTAGTNRALLAVQRLIGWPELDFRGRLFAVAALTGVFASVTS
                     AIADAINGLAVVQLVINLLVAVFSVGMLWLVRVTHRTQLGYVVAVVVVFMVAFPMLFF
                     AGGGYSAGMPAFFVFALVITPLMIRGRTLWVLWFALVGLYTACCLIAYRYPELVTPLP
                     SDSAVVSDVIVSLLISGGAIAVALRQLLRQHERNQELLAQQNQQLSQMDRAKTDFLAI
                     VAHELNT"
BASE COUNT          779 a         1458 c         1441 g          803 t
ORIGIN      
        1 gaccattttg cgggacgggg agttggtcgg tactcatccg gcagctgagc tcagcattca
       61 cgacatcatc caactgatgg ttggccgcaa gatcgccgtc gacatccgac ccgaaccccg
      121 cgccagcacc acgccggtgt tggaggtaga agggctttgc tcggcgacac tgctgaaaga
      181 tgtcagcttc accctggaaa agggcgagat cctcggcttc gccggcctga tgggtgccgg
      241 acgcaccgag acagctcgag cgctgatcgg agccgacgcc aagacgtccg gcacggtgaa
      301 gatcgacggg aagctcgcga acataactca ccccggtgac gcggtgcgaa aggggattgg
      361 gtacctgtcg gaggaccgca agcggtacgg cctgatcttg cagcagaccg ttgccgcgaa
      421 caccgtcctt gcctcgatct ccaagtacac caggtatggg ctgcttcagg acgccgagat
      481 gcgcaaggcc ggcgagaagt gggtcaacgt cctggccacc aagaccccgt cggttcgcca
      541 gctcgtgcgc aacctctccg gcgggagcca gcagaaggtc gtgctggcca agtggctgga
      601 acaggactgc gacatcctca tcttcgatga acccacgcgg ggcatcgacg tcggcgccaa
      661 gcaggagatc tacacccttc tcgaccagct gacgaaggaa gagggcaagt cgatcatcat
      721 gatctcctcc gaaatggagg aagtcctccg tatgtccgac cgcatcgttg tgatgtgcga
      781 cggacgcatc accggcgtcc ttcccaacgc tgaggccacc caggaaaaga tcatggagct
      841 ggccacgcga ttcaacccaa ccgaacacca gattgaggcg tgacccaatg agcaccaaca
      901 ccaccgccgt gtccgcgtcc acgatgaagt gggctatccc cgagcgctac aaggtcttcc
      961 ttcagcaagg gatggtcctg ctggtcgtcg tcctcctcgc cgtgttcttc actatcatga
     1021 aaccgaactt cgccagctgg tcgaacatct cgtcgctcct gctggcctcg gtcgtcaccg
     1081 gcgtccaagc gctcgggctt tctttcgtca tcgcgactgg cggcatcgac ctgacccccg
     1141 gcctgggcat ggcggccacc tcaactttca ccgcgatcct gatcgccgac gctgggctgc
     1201 ggctgccgat ctgggtgggc atcctgggcg gcctcctcgc cggcgcactg atcggactga
     1261 tcaacgggtc gctggtcgcc tacgttcgga tgcagcccat gatcgccacc ctggcgatga
     1321 tgatggtctg tgccggaacg gcgctggtca tagccaacgg caagacgatc tcgctccggt
     1381 cgatccaggg ctgggagtcc atcgccatgg ggcaggtcat cccgggtgtg ccgaacggcg
     1441 tcaccatctt gatcctgctg gcgatcatcg ccggcgtcgt gatgaacaag accgcgatcg
     1501 gacgctattc gctcgcgatc ggctcgaaca aggaggccac caggatctcg ggtgtgaaca
     1561 cccgtcgctg ggaggtgttg gcctacgtca tctgcggtgt gttcaccggc atctccggca
     1621 tcctgatgtc gtcccggctc gccgctgctc agccgaccct cggccagggc tatgagatgt
     1681 acgccatcgc tgcggccgtg atcggtggca cctccctgcg gggcggacgg gcgtccatct
     1741 tcggcgccgt gatcggtgcc gtcaccatcc agaccatctt caacgggctg accgtaatgg
     1801 gcgtccagga ccaatggcag aaggtcgtcc ttggcgttgt cgtcctgatc gccgtcttcg
     1861 tcgacatcat gcggcgcggc ggcaaggaag agtgacgccc caactcaaga actcggccgg
     1921 gtggtccggt cccgagaaag cagtcctccc agaacaaaga aaaggagaac gttcatgaag
     1981 aaattcattg caggcgtggc cggtgcgacc atcgccgtcc tggcgctcag cgcgtgcagc
     2041 gccggtggaa ccgcggccac cagtgcagct gccggcgcca gcagcgctgc cggcggctcg
     2101 atcaaggttt ccctgatcgc gaagggcacc tcggactact ggaccctggt gaagaacggt
     2161 gctgaagccg ccggtaagga tctgggcgtc gaggtgacct acaacgccac cgacacggag
     2221 aacgagggcg acaagcagct gaacctgctc accgccgcga tcaacgacaa gccgcaggga
     2281 gttgcgatcg ctccgcagga cggcgcacag gacggcgcgc ccaccgtgct ggatcaggcc
     2341 aaagccgcga acatcccggt tgcggtgttc gacaccccgt tgaagagctg ggacggtgcc
     2401 atcgccacca tcgcgtccga caacgagggc atcggcgccc aggccgcgga gaacctgtcc
     2461 aagctgatcg gcggcaaggg caaggtcgcc atggtcatcc agggtgtcac tggtaccgcc
     2521 gcactgcgcc gcgacggctt cgcgaactgg atgaaggcca atgcgccgga catccaggtc
     2581 gtcgacatcc agaatggcga ggccgacccg gccaagtccc gcgacaaggc ccaggccatc
     2641 ctgcaggcca accccgacct ggctggcttc gccggcacca gcgactactc gacgatcgcg
     2701 atcgccgatg aggtcgccgc aaagggcggg acggtgaagg tcgtcggtgt cgacgcctcg
     2761 ccggacgtgc tgaccctgct ttctgagggc aagatcgacg gcatcgtcac ccagaacccc
     2821 taccagatcg gctacaagac cgtgtcgact ctggtggacc aggtcaaggg gaaggccccg
     2881 gccgagaaga ccatcgtctc cgaggctgcc tgggtgacca aggacaacat gaacaacccc
     2941 gagatcaagc aaatcacagg ctagttcaca gcgtgacgcc gtgtaggcgt gccaacgcag
     3001 ccgccgccca ttgccgagat cagcatccgc cattggctgg tggtcattgg cgccggcctg
     3061 ctgatgtcgc tgaacgtcct ggtgttcctc gcggccggga tactgctccc gccgctggct
     3121 gagtctctgg gcgtggggct gggccaggtg atggtcttcg tctcgatcaa catggtggcc
     3181 ggcgcgctgg tgctcgccgt cgccggccct ttcctgatcc ggcggttcgg ttcacggtgg
     3241 ctatcgctgc tcggcggtct cgtcaccggc gcgtcgatct tcgcggtctc gctcgtcacc
     3301 gagctgtggc agctgatcgc tgctctcatc gggttcgccc tggtcaccgg gccgaggtcg
     3361 tggcgtccga ccggggctgg ccggcgggta ggtggcgctg cctgatcgcg tgtcgcgatc
     3421 tcggtgtgat ccttcgactt gatcccgctg tgaacgggcg gtctcggttc tggccgtgag
     3481 atcgcgacat ggtcgccccg tccgcagcaa gaaggtgtaa cggccagcca tgacgaaccg
     3541 ggacgcgccg ctgccgtccg cctgactccg tccgccaacc ggtcgataaa ggatccgtta
     3601 ccgcgggacg cgagcgcgcc cctggtgact agcgtcggca gcgcagccgg cggacgtcgg
     3661 gtcggggggc cgaaacgcca gcgggactgt gcatgggtag ccgggtcgtc ccggttgcag
     3721 gggggtaaac cgggacactc ggcgcctggc aggtatcacc tgtcaggcgt gcccgccatc
     3781 agaccagaac gaagtagggt ccggccatgg cctccactgc tggcacgaat cgcgcgctgc
     3841 tcgctgtgca gcggctgatc ggctggcctg aactcgactt ccgcggccgc ctgttcgcgg
     3901 tagccgccct caccggtgtc ttcgcctcgg tcacctcggc catcgctgac gcgatcaacg
     3961 gcctggcggt ggtgcaactg gtgatcaacc tcctggtcgc ggtgttctcg gttgggatgt
     4021 tgtggctggt tcgggtgacc caccggacgc agctgggcta cgtcgtcgcg gtggtggtcg
     4081 tcttcatggt ggcctttccg atgctcttct tcgccggcgg cggctactcc gcaggaatgc
     4141 cagcgttctt cgtgtttgcc ctggtgatca cgccattgat gattcgcggc agaactttgt
     4201 gggtgctctg gttcgcgttg gtcgggctgt acacggcctg ttgtctgatc gcttaccggt
     4261 accccgagct ggtcaccccg ctaccgtcgg attcggcggt ggtctcggac gtcatcgtct
     4321 ccctcctgat ctccggcggc gccatcgccg ttgcgctgcg ccagctgctt cgccaacacg
     4381 agcgcaacca ggagttgctc gcgcaacaga accagcagct cagccagatg gatcgcgcga
     4441 agaccgactt cctcgcgatc gtcgcgcatg agctcaacac c
//
LOCUS       DAOPRQ010000127         4304 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_16492_length_4304_cov_12.174159, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000127 DAOPRQ010000000
VERSION     DAOPRQ010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 4304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4304
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16492_length_4304_cov_12.174159"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            398..568
                     /locus_tag="PKJ61_11810"
     CDS             398..568
                     /locus_tag="PKJ61_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOC14548.1"
                     /translation="MPTQRYETIAQACERTGISRNTLRRRIATGELPAFMTGRRILRL
                     RPEDVDRLLKPR"
     gene            793..1089
                     /locus_tag="PKJ61_11815"
     CDS             793..1089
                     /locus_tag="PKJ61_11815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOC14549.1"
                     /translation="MLPGLRGADPSVLDRIEAGYGLSAEPEAIHPEPFTAPSLFLFGR
                     QDDVVGFEDGWSFRDHYPRASYVVLDAAGHNAHLERPSLTGALISDWLLRMDAP"
     gene            1286..2527
                     /locus_tag="PKJ61_11820"
     CDS             1286..2527
                     /locus_tag="PKJ61_11820"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOC14550.1"
                     /translation="MDDADVLVVGAGLAGLNAARRLERSGLEVVVLEAADVAGGRVRT
                     DLVDGFRVDRGFQVLNPAYPALRDAVDVERLALGRFGRGVVVRTAGGDQVLADPLRMR
                     GTVKALASVVGADPGAALALLRWAAPSVAGRRVIAATTEMTMDGSLDAAGVRGPLRAQ
                     VLAPFLAGVLGDSEGVTSARFVRWLLRWFILGTPGLPAQGMAAVPAQLVHRLRRPVRL
                     GVAVHALTKRGAGWTTTTSAGNFSARAVVVAAGPAASAALTGRPAPRLRGLATWWFAP
                     PEPPTESRFLHVDGLAAGPVVNTAQVSNAQPGYAPAGRHLVQASALWNGDGPAEAEVR
                     AHLTQLYGRPATDWPLVAVHQIPDALPAISPSGWRTGACDDVDGVLVAGDTADASIQG
                     ALASGAAAADLVARRLGAAGA"
     gene            2639..3076
                     /locus_tag="PKJ61_11825"
     CDS             2639..3076
                     /locus_tag="PKJ61_11825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HOC14551.1"
                     /translation="MAQFTVVRDVRAPAGAVFARLVDWDAHSAAIPLTRLHHTGEAAV
                     GQRFVARTGWGRLAFDDPMEVRLLRPPAGDGVGDVGGVVEVAKAGRVIAGRVRWTVTP
                     TQSGSRVEWSQTLVVPWLPKFLDPLVGGVGRLAYTAGLRRILR"
     gene            3117..3692
                     /locus_tag="PKJ61_11830"
     CDS             3117..3692
                     /locus_tag="PKJ61_11830"
                     /inference="COORDINATES: protein motif:HMM:NF016705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HOC14552.1"
                     /translation="MTEQQPYAVVERRADFELRRYPAHLVAQTEVEAPMEAAVNRGFR
                     VLADYIFGANQAKAKVAMTTPVLQQSAGEPIAMTAPVLQAPGDRPGRYVISFVMPAGY
                     TLDTLPTPLDVRVQWREVAAELAAARRFSGLGRAEAFSAELDRLTAAVRAAGLTVVGP
                     ARSARFDPPWKPWFLRRNEVVLPVAGQPSQG"
     gene            complement(3885..>4304)
                     /locus_tag="PKJ61_11835"
     CDS             complement(3885..>4304)
                     /locus_tag="PKJ61_11835"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOC14553.1"
                     /translation="GITKDPALREMNFGGWEGESNLTMQKAVLAKLGTGTFTIRQMLE
                     AIKDTNPVPAMPAENCDDINARALPAMDDIAKDAADGSGGKKVLVVTSGITIACMLDS
                     MGSAIPATGIANGAVNLLEYNNGTWTVKTVNDTHYAG"
BASE COUNT          593 a         1483 c         1506 g          722 t
ORIGIN      
        1 gggaggttca actcctctct cgcgcacaca ttggttatgt gcccctgacg agggcaaaca
       61 gcatctgact agcggtttta ggccgaattg gctctgctca ggtgtaccca ggaagtgcca
      121 tcgggggctc ttcgtgcccc acgtttgccc cacgctgccc cacgtgtgcc ccacggaaat
      181 ggtacggaat ggcataccca aaggggtatc gccggcgcaa cccctcttat gctggatacc
      241 ccctggatgt cgtccagggg gacagccatt cacgcatagc gccgtcgatg gtcccggcct
      301 gtccgggtgc ggctggctct cttctcctca agttcccggc gcctccctgt ccggatcgac
      361 accgttgcag ggcttaagga gtcagagagg agtagccatg cccacccagc gttacgagac
      421 catcgcacag gcttgtgagc ggaccgggat cagccgcaac acgctgcgcc ggaggattgc
      481 caccggcgag ttgcccgcgt tcatgaccgg gaggcgcatc ctgcgcctcc gccccgagga
      541 cgtcgaccgg ctcctcaagc cgcgctaagg ccgcccgatc gcagtctgag gtaccgatat
      601 gagccgaagg caggacaagc gtacgttcgg tgagatctcg ccgttgccga gcggacggta
      661 ccgggcgcgg taccgcggcc ccgacggcga ccggtacacg gcgccggtca cctttctgtc
      721 ccggatggac gccgaaacct ggctgtcggc ccaggatcga aagatcagct cctcgcggcg
      781 ttccttaagt tcgtgcttcc cggtcttcgt ggcgccgatc cgtccgtact cgaccgcatc
      841 gaggccggct acggcctgtc cgccgagccc gaggccatcc accccgagcc gttcaccgcg
      901 ccctcactgt tcctgttcgg tcgccaggac gatgtcgtgg gctttgaaga cggctggtcc
      961 ttccgcgatc actacccgcg cgccagctac gttgtcctgg acgcagccgg tcataacgcg
     1021 catctcgagc gtcccagcct caccggcgcc ttgatcagcg actggctgct gcgcatggat
     1081 gcaccgtgac gccacagcct tgtagaggcc gggacggcgc cgccggggcc agccggccaa
     1141 gcggcgaagc gcaagaccga ttctcggcga gcacactatg tcgagaccga acggtgctga
     1201 acgcaaagca gccgcgacca gatgcgcggg ggctcgctat gctcggcccg cggagcggtt
     1261 tgggcatcag gccaggagga gcggcatgga cgacgccgat gtgctggttg ttggagccgg
     1321 cctggctggc ctgaacgcgg cccgccggct tgagcgcagc ggtcttgaag tggtcgtgct
     1381 ggaggccgcc gacgtcgcgg gcggacgggt gcgcaccgac ctggttgatg gcttccgggt
     1441 tgaccgtggc ttccaggtgc tcaacccggc ctacccggcg ttgcgcgatg ccgtggacgt
     1501 cgagcgcctc gccctgggtc ggttcggacg cggggtggtc gtgcgtacgg ccggtggcga
     1561 ccaggttctg gccgacccgt tgcggatgcg gggaacggtc aaggcgctgg cgagcgtggt
     1621 cggcgccgat ccaggggctg cgttggcgct gctgcgctgg gcggcgcctt cggtcgccgg
     1681 gcgccgagtg atcgctgcga cgaccgagat gaccatggac ggttcgctgg acgcggccgg
     1741 ggtgcgcggc ccgctgcgcg ctcaggtgtt ggcgccgttc cttgccggcg tgctgggcga
     1801 cagcgaaggc gtgacgtctg cccggttcgt gcgctggctg ttgcgctggt tcatcctcgg
     1861 cacgcccggc ctgccggcgc aggggatggc ggctgtgccc gcccagctgg ttcaccggct
     1921 gcggcggccg gtgcggctgg gcgttgccgt gcacgccttg acgaagcgcg gcgcgggctg
     1981 gacgacgacg accagcgcgg gcaacttcag cgcccgggca gtagtagtgg cggccggccc
     2041 cgcggcgtcc gctgcgctca ccggacggcc ggcgccccgc ctgcgcgggc tcgcgacctg
     2101 gtggttcgct ccgccggagc cgccgaccga gagccgattc ctgcacgtgg acggtctggc
     2161 cgcgggcccg gtggtgaaca ccgcccaggt gagcaacgcg cagcccgggt acgcaccggc
     2221 cggacgacac ctggtgcagg cgtccgcgct gtggaacggc gacggcccgg ccgaggcgga
     2281 ggtgcgtgcg cacctgaccc agctttacgg ccgtccggct actgactggc cgctggtcgc
     2341 cgtccaccag atcccggacg cgctgccggc catcagcccc tcgggttggc ggacgggcgc
     2401 atgcgacgac gtggacgggg tgctggtcgc cggcgacacc gccgacgcct cgatccaagg
     2461 cgccttggct tcaggcgctg ccgccgccga cctcgttgct cgccggctcg gtgcggccgg
     2521 cgcctgagcg ctgggccact gctcctcgcg accggttcag cgctgccgta caactgcggc
     2581 cacaccccgg ccttctaccg cactcgccac caggcaggcc caacggcgac gattgggtat
     2641 ggctcagttc acggttgtcc gagacgtccg cgcgccggcc ggggcggtgt ttgcccgact
     2701 ggtcgactgg gatgcccaca gcgctgccat cccgctcacc cggctgcacc acaccggcga
     2761 ggcggccgtg ggtcagcggt tcgtggcccg caccggctgg ggccggttgg cattcgacga
     2821 tccgatggag gtgcgcctgc tgcgtccgcc cgctggggac ggcgtcggtg acgtgggcgg
     2881 ggtggtcgaa gtggccaagg ccggccgggt catcgccggc cgggtgcgct ggacggtgac
     2941 gccgacgcaa tccggctccc gcgtggaatg gtcccaaacg ctcgttgtcc catggctgcc
     3001 gaagttcctc gacccgcttg tcggcggggt cggtcgactc gcctacaccg cggggctgcg
     3061 taggatcctg cgctgaaccg gtccggcagc attgggcagg ggtgcacgct tgggttatga
     3121 ccgagcagca gccctatgcg gtggtggagc gtcgcgcgga cttcgagttg cgccgatatc
     3181 cagcccattt ggtcgcccag accgaggtcg aggcaccaat ggaggcagcc gtcaaccgcg
     3241 gcttccgggt actggccgat tacatcttcg gcgcgaacca ggccaaagcc aaggtcgcga
     3301 tgaccacacc cgttctgcag cagtccgctg gcgagccgat cgccatgacc gcacccgtcc
     3361 tgcaggcgcc aggtgatcgg cccggacggt acgtgatctc gttcgtcatg ccggccggct
     3421 acaccctcga tacgcttccc accccactcg acgtccgcgt ccagtggcgc gaagtcgccg
     3481 ccgagcttgc ggctgcccgg cggttcagtg ggctcgggcg tgcggaggct ttcagcgcgg
     3541 agctcgaccg gctgaccgca gcggtgcgag ccgccggctt aaccgttgtc ggaccggccc
     3601 gcagtgccag gttcgacccg ccctggaagc cctggttcct gcggcgaaac gaggtcgtcc
     3661 tgcccgtggc gggccagccg tcgcagggct gaccacaggg cactgcgttt caccgcgcac
     3721 tgaaccgcga ggcgatcacc cgcgtgccga cccgccccgc tcgccgaaac gggtggtaac
     3781 cacggtcggc gttgcgtcca gaccccgcca gctcccggag tggccctgcg cgcatggcaa
     3841 gcgaaagggt cgcccgcttc cgagcgggcg gcctttcctg ctggtcagcc ggcgtaatgc
     3901 gtgtcgttga ccgtcttcac cgtccaggtg ccgttgttgt actcgagcag gttcaccgca
     3961 ccattggcga taccggttgc cggaatggcc gaccccatcg agtcgagcat gcaggcgatc
     4021 gtgatcccgc tggtcacgac caacaccttt ttgccgccgg agccgtcggc tgcgtccttc
     4081 gcgatgtcgt ccatcgccgg gagcgcgcgg gcgttgatgt cgtcacagtt ctcggctggc
     4141 atggcgggca ccgggttggt gtccttgatc gcctcaagca tctggcggat ggtgaacgtc
     4201 cccgtgccga gcttggccag caccgccttc tgcatggtca ggttcgattc gccttcccag
     4261 ccgccgaagt tcatttcgcg cagcgcggga tccttggtga tgcc
//
LOCUS       DAOPRQ010000128         4205 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_17007_length_4205_cov_12.184819, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000128 DAOPRQ010000000
VERSION     DAOPRQ010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 4205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4205
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17007_length_4205_cov_12.184819"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="PKJ61_11840"
     CDS             complement(<1..203)
                     /locus_tag="PKJ61_11840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14554.1"
                     /translation="MSVLGEWAEATDDNRPSVGTVSVAGVSVDVTGLAQDLVRKTILS
                     EVRAQVRDVVTPVIADAVAKALTP"
     gene            complement(200..2038)
                     /locus_tag="PKJ61_11845"
     CDS             complement(200..2038)
                     /locus_tag="PKJ61_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015070189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HOC14555.1"
                     /translation="MTTANNRRTEPGRAAGETVLLWVGLGVGGFALLLVWVAVKAGHR
                     LAGLPDTTNDPFELVVGLVRGKVAWPLASTIVAVGLVVIVLAVAIAGAVAWSRRRRQS
                     SRVDHAARYLGRGQDVAPLSRQNAEATANRLGMATGAPKKRAAADARPVTVMPVGVPI
                     GITVSGGQRLYGTWEDMHLDIWGPRTGKTTSRAVPAILDAPGAVFATSNKRDLVDATR
                     DVRESAGAVWVFDPQGIALEEPTWWWNPLSYVVDEVRAAKLAEHFASGSREPGARTDA
                     YFDPAGQDLLAGLLLAAALDGRAITQVYAWLTRPTEDEAVAILERHGFQLTADQVAGV
                     IAAPDKQRGGVYGTAQQMASCLTNRQVARWVTPAGVGFDPRPQFSPERFLREGGTLYS
                     LSKEGRGTAGPLVTALTVAVVEAAEELAARSSGGRLPVPLLGVLDEAANVCRWRELPN
                     LYSHYGSRGIVLMTILQSWSQGVDVWGESGMRKLWSASNVKVYGGGVDESEFLGHLAT
                     VIGDYDKETSSVSFGKGHRQVSQQLHRERILDVADLAAMPKGRAIVMASGARPTLIRT
                     CPWMAGRYAAAVQASITAHDPEASRTLHDATVSLAAAEASADAAGQ"
     gene            complement(2035..2979)
                     /locus_tag="PKJ61_11850"
     CDS             complement(2035..2979)
                     /locus_tag="PKJ61_11850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14556.1"
                     /translation="MAALIAILRNDPPNDREETILDRALRVLDDRHHHGVPVLADLLR
                     VIQEGPEDVRMVALDRGDNDRYQAITENLEASLIGLVGGGRLGRTFSEQTTNPMRRDR
                     AVVYDVSGIDDSEVELQAATLLACWSAGFGTVNVANALADAGLEPRRHYFVILDELWR
                     ALRAGRGMVDRVDALTRLNRQRGVGMAMISHTMSDLMALADESDRMKARGFVERSGMV
                     ICGGLPSAEMPMLTSAIPMSQAEQDLLIGWQDPPAWDPSTNREAAPPGRGKFLVKVGG
                     RPGIPVDVQLTSIEMSVNDTNKVWHTQSRRSAVEEAIA"
     gene            complement(3585..>4205)
                     /locus_tag="PKJ61_11855"
     CDS             complement(3585..>4205)
                     /locus_tag="PKJ61_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015070192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14557.1"
                     /translation="AYDPHAQQWIDECHAQGNPTGLDWSDVGPGAAESTWDGYRHEGA
                     FSITWAMTSAPRGNVQSGVLARILAPHRDIARKRVTILYKPIDAARAAAIVEADLRSA
                     EFRASSANRPSARDSLAVRQAATTASEEAAGAGLVNFGLLVTATVLDVDQAPEARAAI
                     DNLSATARLRLRPMYGSQDVGFIGALPLGLILTKHLRVPTEVRDRL"
BASE COUNT          592 a         1453 c         1476 g          684 t
ORIGIN      
        1 ggcgtgaggg ccttggcgac cgcgtccgcg atcacgggcg tgaccacgtc ccgcacctgt
       61 gcgcggacct cgctgaggat cgtcttgcgg actaggtctt gagccagccc tgtgacgtca
      121 acgctcacgc cggcgacgct gaccgtgccc acggagggcc ggttgtcatc ggtggcttcg
      181 gcccactcgc ctaggacgct cattggcctg cggcgtccgc tgacgcttcc gcggccgcca
      241 ggctcacggt ggcgtcgtgg agcgtcctag acgcttcagg atcgtgcgcg gtgatggagg
      301 cttggaccgc agcggcgtac cggccggcca tccagggaca ggtgcggatg agggttggcc
      361 gtgccccgga ggccatgacg atcgcgcggc ccttgggcat cgcggccagg tcggcgacat
      421 cgaggatgcg ttcgcggtgg agctgctggg acacctgccg gtggcccttc ccgaaggaga
      481 ccgacgatgt ctccttgtcg tagtcaccga tcacggtggc caggtggccc aggaactcgc
      541 tctcgtcgac gccgccgccg tagaccttga cgttactcgc gctccagagc ttccgcatcc
      601 cgctctcgcc ccagacgtcg acgccttggg accagctctg caagatcgtc atgaggacaa
      661 tccctcggga gccgtagtgg ctgtagaggt tcggcagctc acgccaccgg cagacgttgg
      721 cggcctcgtc caggacgccg agcagcggca ccggcaaccg gccaccggag gagcgggcgg
      781 ccagctcctc ggcggcctcc acgacggcca cggtgagcgc cgtgacgagc ggccccgccg
      841 ttccgcggcc ttccttgctc aggctgtaca gggtcccgcc ttcacggagg aatcgttccg
      901 ggctgaactg tggccgggga tcgaagccga cgcctgccgg cgtgacccac cgggcgacct
      961 gtcggttagt caaacaacta gccatctgct gcgcggtccc gtagacgcct ccccgctgct
     1021 tgtccggggc ggcgatgacg ccggcgacct ggtctgcggt gagctggaac ccgtgccgtt
     1081 ccaggatggc tacggcctcg tcttcggtcg gccgggtcag ccaggcgtac acctgcgtga
     1141 tcgcacgccc atcgagcgcc gcggccagca gcaggccggc caggaggtcc tggccggccg
     1201 ggtcgaagta ggcgtccgtg cgggcgccgg gctcgcgtga tccggacgcg aagtgctcgg
     1261 ccagtttggc ggctcggact tcgtcgacga cgtaggacag cgggttccac caccaggtcg
     1321 gttcttccaa ggcgatccct tgcggatcga agacccacac cgcgccggcg ctctcgcgga
     1381 cgtcgcgggt cgcgtcgacc aggtcgcgtt tgttcgaggt cgcgaagacg gctccgggtg
     1441 cgtccaggat ggccggtacg gcccgcgacg tcgtcttgcc ggtgcgggga ccccagatgt
     1501 ccaggtgcat gtcctcccag gtgccgtaga gccgttggcc gccgctgacg gtgatgccga
     1561 tcggcacccc gaccggcatc accgtgacgg gacgggcgtc ggcggccgca cgcttctttg
     1621 gcgctcccgt ggccattcct agccggttcg cggtcgcttc ggcgttctgc cgcgacaacg
     1681 gtgcgacgtc ctgaccgcgg ccgagatacc gagccgcgtg gtcgacccgg ctggactgcc
     1741 gacgacggcg agaccaggcg accgcgccgg cgatcgcgac agccagcacg atcaccacca
     1801 ggcccacggc gacgatcgtg gacgccagcg gccaggcgac cttgccgcga accaatccga
     1861 cgaccagctc gaacggatcg ttcgtggtgt ccggcaggcc cgcgagccgg tgccctgcct
     1921 tgaccgctac ccacaccagc aggagagcga acccgccgac cccgagaccg acccacagca
     1981 ggacggtctc gccggccgcg cggcccggct cggttctgcg gttgttcgcg gtggtcacgc
     2041 gatcgcctcc tcgaccgcgc tgcggcggga ttgcgtgtgc cacaccttgt tggtgtcgtt
     2101 gacgctcatc tcgatgctgg tcaactgcac gtcgaccgga atccccggtc gtccgcccac
     2161 cttgaccaag aacttcccgc ggcctggggg tgcggcttcc ctgttggtgc tggggtccca
     2221 cgccggcggg tcctgccatc cgatgaggag gtcttgttcg gcctgcgaca tcgggatggc
     2281 ggaggtgagc atcggcattt cggcggacgg cagcccgccg cagatcacca tgccggaccg
     2341 ctcgacgaat ccgcgcgcct tcatcctgtc cgattcatca gccagcgcca tgaggtcgct
     2401 catcgtgtga gagatcatcg ccatcccgac gccgcgttga cggttcagcc gtgtcagcgc
     2461 gtcgacccgg tcgaccatgc cccggccggc gcgcagcgcc ctccacagtt cgtcgaggat
     2521 gacgaagtag tgtcgccgcg gttcgaggcc ggcgtccgcc agggcgttcg cgacgttcac
     2581 cgttccgaag ccggccgacc agcatgccag cagggtcgcg gcttgcagtt cgacctcgga
     2641 gtcgtcgatt ccgctgacgt cgtagacgac agcgcggtcg cggcgcatcg gattggttgt
     2701 ctgctcggag aatgtgcggc cgagtcggcc accgcccacc aggccgatca gtgaggcttc
     2761 caggttctcg gtgatggcct ggtaacggtc gttgtcgccg cggtcgaggg cgaccatgcg
     2821 gacgtcctca gggccttctt ggatcacgcg cagcagatcg gccagcaccg gcacgccgtg
     2881 gtggtggcga tcgtcgagga cgcgaagagc ccgatcgaga atcgtctctt cgcggtcgtt
     2941 cggcggatcg ttgcgcagga tggcgatcag ggcggccacc atggtgagcc gtcgaccgtg
     3001 ggcgtcggcc aggacagcgg cgcgcgctga cccggtcagc ctgtcggcgg cttcgtgggc
     3061 ttcgccgggg tcgaggacgt tgatcgaacc gcggccgcgg ccgatcggaa tcacctggcc
     3121 gcccagggct tcgatcaggt cgacgtagtc aggtttcagg tcaccgagga ccaatgggag
     3181 ggcgccgaac ccggccagcc cggtcgccat gcgacggacc atcgtggatt tcccgagacc
     3241 cggtttgcct agcacgaagg ccgaggggtt gctgatcagc ttggcccgct ggaaccagct
     3301 gatcgggtcg cagcacagcg ttgcgccggt cccgatgtga cggccgaccg gcacgccaac
     3361 cattggcgtc cctgtgccga cgacccacgg ccacagccca cagacttgca cggtcgtgcc
     3421 gcgccactca tctggtgcct cgacgagcac ggactgaccg gctccccggc cgtaccagcc
     3481 ccgcgaggct ggtcggagcg gccgcagcgg agcgtgacga ggctgtgact cctcgccgcg
     3541 ccacttgcgg ccggcccgct tcccggcttc gttcggcttc gcggtcacag tcgatcacgc
     3601 acctcggtcg gcacccgcag gtgcttggtc aggatgaggc ccagcggcag cgcgccgatg
     3661 aagccgacgt cctgggaccc gtacatgggc cgcaggcgca gtcgtgccgt cgccgacagg
     3721 ttgtcgatcg ctgcgcgcgc ctcgggcgcc tggtcgacgt cgaggacggt tgcggtcacc
     3781 aggagaccga agttgaccag accagcgccg gcggcttcct cggaggccgt tgtggcggcc
     3841 tggcgtaccg ccagcgagtc ccgcgctgac ggccggttcg ctgagctcgc ccggaactcg
     3901 gcgctgcgca ggtcggcctc gacgatcgcc gcggcgcggg cggcgtcgat cggcttgtag
     3961 aggatcgtga cccgcttgcg ggcgatgtcg cgatgtggcg ccaggatgcg tgccaggacg
     4021 cccgactgga cgttgccgcg tggggccgat gtcatcgccc acgtgatgct gaacgcgccc
     4081 tcgtggcggt agccgtccca ggtcgactcg gctgcgcccg gcccgacatc ggaccagtcc
     4141 aggcccgtgg ggttgccttg cgcatggcac tcgtcgatcc actgctgggc gtgcgggtcg
     4201 taggc
//
LOCUS       DAOPRQ010000129         3965 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_18250_length_3965_cov_10.342967, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000129 DAOPRQ010000000
VERSION     DAOPRQ010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 3965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3965
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18250_length_3965_cov_10.342967"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="PKJ61_11860"
     CDS             <1..556
                     /locus_tag="PKJ61_11860"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOC14558.1"
                     /translation="LLFTRRGLAYSDELRAEVFGKSVPATAALLAGPLGEVGNESALV
                     EEMLTAVADVIAAEVRPMPGALELLTHLHGRVPIAVASNSPRYLVELALGRAGLRPFF
                     EVLVSRDDVSAGKPAPDLYVEACARLGVNPAEAVAFEDSLVGLASARAAGLRVVGVPS
                     VRGQEFPADLVVDSLADARLLSWL"
     gene            complement(781..>1611)
                     /locus_tag="PKJ61_11865"
     CDS             complement(781..>1611)
                     /locus_tag="PKJ61_11865"
                     /inference="COORDINATES: protein motif:HMM:NF027952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HOC14559.1"
                     /translation="SMAILQANPQFTSYTLISDASHIRRAAVLFDAASVAIQSRTGAA
                     WAMTARGNVAFSDKAITAQASDATHSVITSNVASVFGVLSAYSALLKTPPGTPVLKSI
                     KVTPPTVLKYAVGQKMNATGLVVTAYYNVTGLTKVVTANAKISGYSSTKTGKHTVHVS
                     YTENGVTKSTTFTTLVGKAASRVALTASKSTLKVKKTRATVTADVVSATGVVPTGKVK
                     FYLGKKLVKSVKLKSGQTTVKVKYPAVEKKGTYKVTVKYSGSSLLEKSQKSLKLKVKK
                     "
     assembly_gap    1612..1711
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2020..2436
                     /locus_tag="PKJ61_11870"
     CDS             2020..2436
                     /locus_tag="PKJ61_11870"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOC14560.1"
                     /translation="MDSLVALAADLRIACQRVSRRVRFESSTEIAPHLVSAMANLRHG
                     PLTPGELAEVERISAPSMTRTVNCLVDKGLVAREDHPSDGRCKVLTLTASGHDLLDRV
                     AQARNDWMVHQLEGLTDAERQLLREATDLLTQVVSK"
     gene            2433..3719
                     /locus_tag="PKJ61_11875"
     CDS             2433..3719
                     /locus_tag="PKJ61_11875"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOC14561.1"
                     /translation="MTVTEERVPMFASMGLRNYRLFWTGGFISNIGTWMSRIAQDWLV
                     LTILTQNSAVALGLVTALQFLPIAVLAPYAGSLADRFSKRRVLMITQVAQAAVGLTLA
                     GLVLGGAVQLWHVYVLAALLGIAGAFDGPARQAMAPEMVPETLIPNAVGLNTTSFHAA
                     RLIGPAMAGLSIAWWGVGPALVFNGLSFVAVLVALAMMDEAELTPSPRAKAKGSTMAG
                     LRYVRSRPDIMLLMFLVFMLGTFGLNFQITNALMATTVFKVGADSYGVLSSIMACGSL
                     AAGLVAAKRTQLRLRLIMGALTLFALSCLLLAVAPNYLWYALLLVPAGFTSLTVMTAA
                     NASVQLSTTPVMRGRVMAIYQAIFLGGTPLGAPLIGWVGDAWGPRWTVAIGGIMCGVA
                     VVVAIAYLVADEGWDVMPWRNRRPDAVLVEDFEEAR"
     gene            complement(3727..>3965)
                     /locus_tag="PKJ61_11880"
     CDS             complement(3727..>3965)
                     /locus_tag="PKJ61_11880"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756059.1"
                     /note="activates fatty acids by binding to coenzyme A;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HOC14562.1"
                     /translation="DAAVIGVPAKSGGERVVAVVIPRPGTTIDEAAMRDWCRERLAGY
                     KVPRQIVAIDDLPRSMLGKVLRKQVRDDFLANHA"
BASE COUNT          550 a         1282 c         1299 g          734 t
ORIGIN      
        1 gctgctgttc acccgtcgcg gcttggcgta ctcagatgag ctgcgcgccg aagtgttcgg
       61 taagagcgtc cctgcgaccg ccgcgctgct agccggaccg ctaggcgagg tcggcaacga
      121 atcggcactc gtggaagaga tgctcaccgc ggtcgcggac gtgatcgcag ccgaggtgcg
      181 gccgatgccc ggcgccctgg agttgctcac gcacctgcac ggccgggtgc cgatcgctgt
      241 cgccagcaat tcgccgcgct atctggtgga gttggctctc ggccgcgcgg ggctgcgtcc
      301 gttcttcgag gtgctggtct cccgcgacga cgtgtccgcc ggcaagccgg ccccggatct
      361 ctacgtcgag gcctgcgccc ggctcggcgt caatccggcc gaggcggtgg cgttcgagga
      421 ctccctggtc ggcctggctt cggcgcgagc cgcaggcctg cgggtggtcg gcgtgcccag
      481 cgtccgcggg caggagttcc cggccgacct tgtggtcgac tcgctggccg acgcccggct
      541 gctcagctgg ctctgacccg acttggatgg cttacgggag cttgaggttc caagcagttg
      601 aaccaaaccc gacttcggag gcttaaccga ccccaagtga gccggttaag ccatccaact
      661 cgggttacgg ccgtcagcgc tgtcgccacc ggcttggtat gcctcccaat tcgggttacc
      721 ggtgtgccgc cggcgctcag ctcgaggtgt gcctcgtgag gctggcaccc gggtgggtgt
      781 ttacttcttc accttcaact tcagcgactt ctgcgacttc tccagcagcg agctgccgga
      841 gtacttgacg gtgaccttgt aggtcccctt cttctcgacc gcggggtact tcaccttgac
      901 cgtcgtctgg ccggacttca gcttcaccga cttgaccagc ttctttccca ggtagaactt
      961 caccttccct gttggtacga cgccggtggc ggagaccacg tccgcagtga ccgtggcccg
     1021 ggtcttcttc accttcagcg tcgacttgga cgcggtcagc gccaccctgc tggccgcctt
     1081 gccgaccagg gtggtgaagg tcgtcgactt ggtgacgccg ttctcggtgt agctcacgtg
     1141 cacggtgtgc ttgccggtct tggtcgagct gtagccggag atcttcgcgt tcgccgtgac
     1201 caccttggtc aacccggtca cgttgtaata ggccgtcacg accagcccgg ttgcgttcat
     1261 tttctgtccg acggcgtact tcaacaccgt cggcggtgtg accttgattg acttcagcac
     1321 cggggttccg ggcggcgtct tcagcagcgc actgtaggcc gacaacaccc cgaacacact
     1381 ggccacgttg ctggtgatga cgctgtgcgt ggcatcggac gcctgggctg tgatcgcctt
     1441 gtccgagaac gcgacgttgc cccgggcggt catcgcccag gcggcgcccg tccggctctg
     1501 gatcgcgacg gacgcggcgt cgaacagcac ggccgcgcgc cggatgtggg acgcgtccga
     1561 gatcaacgtg tagctggtga actgcgggtt ggcctgcagg atggccatcg annnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncaggtcgcc gacgcgctgg tagtagagcg
     1741 cacgcgtcct caggtcggtg acgctcaccg tggccgcctg ggccggggtc gtggcgggcg
     1801 cgaccagtcc cagcagcaac gccaggctca ccagtaacac tcgaaccgtt gcgttgaact
     1861 gcttcatcac acgtccttac ggcggggtcg tgcagcggtg cgtcgacgcg tccggctgca
     1921 ggagttgggg accttgggtc ttcaacgtag ctaccgatcg cgattcgcgt ggatttggtt
     1981 gagagttagt tagctgaact aatgagctaa gctaaggata tggattccct ggttgccctt
     2041 gccgcagatc ttcggatcgc ctgtcagcgg gtcagccgac gcgtccgctt cgaatcgagc
     2101 accgagatcg caccgcacct ggtgagcgcc atggccaacc tccggcacgg cccgctcact
     2161 cccggcgaac tcgccgaggt tgagcggatc agtgcgccga gcatgacccg aaccgtgaac
     2221 tgcctggtcg ataaaggcct cgtcgcgcgc gaggaccacc ccagcgacgg tcgctgcaag
     2281 gtgctcacgc tcaccgcgtc cgggcacgac ctgctcgacc gagtggcgca ggcgcggaac
     2341 gactggatgg tgcaccaact cgaagggctc accgacgccg agcggcagtt gctgcgcgaa
     2401 gccaccgacc tgctgaccca ggtggtgagc aagtgaccgt taccgaagag cgggtcccga
     2461 tgttcgcttc gatgggccta cgcaactacc ggctgttctg gacgggcggc ttcatctcca
     2521 acatcggcac ctggatgtcc cgcatcgctc aggactggct ggtgctgacc atcctcaccc
     2581 agaactcagc ggtcgcgctg ggcctggtca ccgcgctgca gttcctgccg atcgcggtgc
     2641 tcgcgccgta cgccggctcg ctcgccgacc gcttctccaa gcgcagggtg ctgatgatca
     2701 cccaggtcgc gcaggctgcg gtcggcctga ctcttgccgg gctggtgctc ggtggcgcgg
     2761 tgcagctctg gcatgtgtat gtgctggccg ctctgctcgg catagccgga gccttcgacg
     2821 gtccggcccg gcaggcgatg gcgccggaaa tggtgcccga gaccctgatc cccaacgcgg
     2881 tcgggctgaa cacgaccagc ttccatgcgg cccggctgat cggtccggcg atggccgggc
     2941 tgagcatcgc ctggtggggc gtcggcccgg cgctggtgtt caacgggctg agcttcgtcg
     3001 ccgtcctggt cgctctggcg atgatggacg aagccgaact caccccgtcc ccgcgggcca
     3061 aggcgaaggg ctccacgatg gccggtctgc gctatgtgcg ctcgcgtccg gacatcatgc
     3121 tgctgatgtt cctggtgttc atgctgggga cgttcgggct gaacttccag atcaccaatg
     3181 cgctgatggc taccaccgtg ttcaaggtgg gcgcagactc ctacggcgtg ctcagctcga
     3241 tcatggcctg cggctcactg gccgccgggc tggtcgcggc gaagcggacg cagttgcggc
     3301 tgcggctgat catgggcgcg ctgaccctgt tcgcgctgtc gtgcctgctg ctcgcggtgg
     3361 cgcccaacta cctgtggtac gccctgctgc tggtgccggc cggtttcacg tcgctgactg
     3421 tgatgaccgc cgcgaacgcg tccgtccagc tgtcgaccac gccggtgatg cgcggccggg
     3481 tgatggcgat ttaccaggcg atcttcctcg gcgggacgcc gctcggcgcg ccgctgatcg
     3541 gctgggtcgg cgacgcttgg ggcccgcgct ggacggtggc catcggcggc atcatgtgcg
     3601 gcgtggccgt cgtcgtcgca atcgcgtacc tggtcgctga cgaaggctgg gacgtcatgc
     3661 cctggcgcaa tcgccgtccg gacgccgtcc tggtggagga cttcgaagag gcccgctgaa
     3721 cggcgttcag gcgtggttgg cgaggaagtc gtcacgaacc tgcttgcgca gcaccttgcc
     3781 gagcatcgag cgtggcaggt cgtcgatcgc gacgatctgg cggggcacct tgtagccggc
     3841 cagccgctcc ctgcaccagt cgcgcatggc cgcctcgtcg atcgtcgtgc ccggacgcgg
     3901 aatcaccacc gccaccaccc gctcgccgcc ggacttcgcc gggacgccga tcacggccgc
     3961 gtcca
//
LOCUS       DAOPRQ010000130         3910 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_18563_length_3910_cov_13.606744, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000130 DAOPRQ010000000
VERSION     DAOPRQ010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 3910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3910
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18563_length_3910_cov_13.606744"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(119..1249)
                     /locus_tag="PKJ61_11885"
     CDS             complement(119..1249)
                     /locus_tag="PKJ61_11885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14563.1"
                     /translation="MSQVEERLREDLLRATDFAVEVDAEAVLGAGRRDRSRRFVRTAG
                     VGLVATVAVGLLALAGLGGRLNFGGTEVGATVAASAAPAPTAAPGTASFDVLQQFLSK
                     SVTYTDEGEVTASPQVKSEMPMRVEVTPQGAGRYQLAVTTVVGERTEEFVQVEAGGSV
                     MTALLPEVLVGVIRADARWVDLRPLEVSAAGGWLDTGMVGESGDATAFVMAFPEDGTA
                     AGEVRGVTWADADGRVHDSSGVSVVTAKVGLGEAEGLVYLEPEGDGVRYHDDAARELG
                     VGMQDAEVSFRAVASWSESDSGVEEAGLGVLPKGATDAEFKWLDGVVEKSASTVRLGD
                     REVFVVSAKLPTRVEPVTMDGGLKSVEYTTADGTRGTYRPGD"
     gene            complement(1246..1758)
                     /locus_tag="PKJ61_11890"
     CDS             complement(1246..1758)
                     /locus_tag="PKJ61_11890"
                     /inference="COORDINATES: protein motif:HMM:TIGR02983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="HOC14564.1"
                     /translation="MFGSSTRRARDAEFVAFVKQASAGLTRTAYLLTGDRETARDLVQ
                     EALVRTYRAWGRVRTEEAGAYARRVLVNLNIDRWRRRPAEPRPEVERIDPADAMARVD
                     DRDEVVRMLSRLPAQQRKVIVLRYFGDLTEARTAQVLGISVGTVKSASSRGLESLRSL
                     YVPVEEGERS"
     gene            complement(1849..2898)
                     /locus_tag="PKJ61_11895"
     CDS             complement(1849..2898)
                     /locus_tag="PKJ61_11895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14565.1"
                     /translation="MIDLEERLRAELNGLRTRDLDLGVATDDVIASARRVRGRRQLRG
                     AISLVAAAAAIGVVAMPLAGMLRGTDQPVPATPAGPSTSTESGLGRVEFDLAQYELGD
                     DFASLGVAVRRVGDRVEATATRYSASGMMQAQFGPAISPSVEEALWFSVGPRVEFGVI
                     SGVATWVAPRGLSAERIMVTKDLPELDATVYLTIAPKGEANQTTIMPNVLWQDRNGAV
                     RSGAAGQLPSTAIDVAGNRGTAYLDLGADEFGFVASTGNSWRFKASEAAKRTRCYFSG
                     EELDGKWDAAVFCLLPPGAQNPEPELVDATKHALADVGGRKLLVMPGSADSIKGIVTS
                     VSYTNTDGQTVTDPV"
     gene            complement(2898..3401)
                     /locus_tag="PKJ61_11900"
     CDS             complement(2898..3401)
                     /locus_tag="PKJ61_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020576379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="HOC14566.1"
                     /translation="MLGSSKRGRDAEFTEFVRSASKSLSWTAYLLTGEKDAAAEVLQE
                     ALVKTYLAWPRVRHGEAAAYARRTLVNLSIDRWRRRPARPTDDYDRPDLRDAERVIDD
                     RDELVRMLAGLPERQRRVIVLRYFEDLSEAAVAQCLGISVGTVKSAASRGLATLRAAH
                     APLGGER"
     gene            3564..>3910
                     /locus_tag="PKJ61_11905"
     CDS             3564..>3910
                     /locus_tag="PKJ61_11905"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HOC14567.1"
                     /translation="MRVALAQLIASTDPAANLNQVRAHIKRAVAAGAELLVLPEATMC
                     SFSRPRADTAEPFDGPWATAVRAAAAAAGLTVVVGMFTTTNTARVANTLLATGVVEAR
                     YDKMHLFDALGYA"
BASE COUNT          654 a         1632 c         1018 g          606 t
ORIGIN      
        1 tgaacccaac ccgctttcca agcgagcgcc ataggcctct aggcgaatcc tccgccggtt
       61 acgataccgg gttcctcggg cagaactcca accagcgccc agaccacccg gccaaccctc
      121 agtcaccagg acggtaggtg cccctcgtcc cgtccgccgt ggtgtactcg accgacttca
      181 ggccgccgtc catcgtgacc ggctcgactc tcgtcggcag cttcgcactc accacaaaca
      241 cctcgcggtc acccagtcgc accgtcgatg ccgacttctc caccacacca tccagccatt
      301 tgaactcggc gtcggtcgcg cccttgggca atacccccag tccggcctcc tccacaccag
      361 aatccgactc cgaccagctc gcgacggctc tgaaactcac ctcagcatcc tgcatcccga
      421 cccccaactc ccgcgcagcg tcgtcgtgat accgcacccc atcaccctcc ggctccaggt
      481 acaccaaccc ctcagcctcg cccagcccca ccttcgcagt caccactgac accccggagc
      541 tgtcatgcac ccgaccgtcc gcgtccgccc acgtcactcc gcgcacctcg ccggctgccg
      601 tcccgtcctc ggggaacgcc atcacgaacg ccgtggcgtc acccgactcg ccgaccatgc
      661 ccgtgtccaa ccagccaccg gccgcactca cctcgagcgg acgcagatca acccagcgcg
      721 cgtccgcgcg gatcaccccg accagcacct caggcaacag cgccgtcatc accgacccgc
      781 ctgcctcgac ctggacgaac tcctccgtcc gctcccccac caccgtcgtc accgccaact
      841 ggtaccgccc agcaccctgc ggcgtcacct ccacgcgcat cggcatctcc gacttcacct
      901 gcggactcgc cgtcacctca ccctcatctg tatacgtcac cgatttcgac aagaactgct
      961 gcagcacatc gaagctcgcc gtcccgggcg cggccgtcgg cgccggtgcc gcggacgcag
     1021 ccaccgtcgc ccccacctcc gtcccgccga agttcagccg tccacccaag cccgccagcg
     1081 ccagcaatcc caccgccacc gtcgccacca gcccgacacc cgccgtccgg acgaaccgcc
     1141 tcgaccgatc cctccggccg gcccccagca ccgcctccgc atccacctcc accgcgaagt
     1201 ccgtcgcccg caacaggtcc tcgcgcaacc gctcctccac ctggctcatg accgttcccc
     1261 ctcctcaacc ggcacgtaca gactccgcag cgactccagg ccacgagacg acgccgattt
     1321 caccgtcccg acactgattc ccagaacctg tgccgttctc gcctcggtca ggtcgccgaa
     1381 gtaccgcagc acaatcacct tccgctgctg cgccggcagc ctggacagca tccgcaccac
     1441 ctcatcgcgg tcgtccaccc tcgccatcgc atccgccgga tcgatccgct ccacctccgg
     1501 cctcggctca gccggacgcc gccgccagcg atcgatgttc agattcacca gtacacgcct
     1561 ggcgtacgcg ccggcctctt ccgtccggac gcgtccccat gcccggtacg tccgcaccaa
     1621 cgcctcctgc accaggtcgc gcgccgtctc ccgatcaccg gtcagcaggt acgccgtccg
     1681 ggtcagcccg gcggacgcct gcttcacgaa cgcgacgaac tcagcgtcgc gcgcccgtcg
     1741 cgtggatgaa ccgaacatct aggctcctgt cccttcatcc ttagctacgc acactacgga
     1801 gggatggttg cctacgggtc ccgaccagct cgaccaaccg aaagtgggct agaccggatc
     1861 ggtcaccgtc tggccgtccg tgttggtgta gctcaccgac gtgactatgc ccttgatcga
     1921 atcggcgctg ccgggcatca ccagcagctt tcggccgccc acgtccgcca gcgcgtgttt
     1981 tgtcgcgtcc acaagttcgg gttccggatt ctgcgccccg ggcggcagca ggcagaacac
     2041 cgccgcgtcc cacttgccgt ccagctcctc gcccgagaag tagcaccgcg tccgcttcgc
     2101 cgcttcggac gccttgaagc gccagctgtt gccggtggat gccacgaaac cgaactcgtc
     2161 ggcgccgaga tctaggtacg ccgtcccgcg atttccggcg acgtcgatcg ccgtcgaagg
     2221 caactgccca gccgcgcccg agcgcaccgc accattccgg tcctgccaaa gcacattcgg
     2281 catgatggtg gtttggttcg cttcgccttt cggcgcgatc gtcaggtaga ccgtcgcatc
     2341 tagttcaggc aggtccttgg tgaccattat ccgctcggcg ctcagccctc gcggcgccac
     2401 ccaggtcgcc acgccactga tcaccccgaa ctcgacccga ggacccaccg agaaccagag
     2461 cgcctcctcc actgacggcg aaatcgccgg cccgaactgc gcctgcatca tcccggacgc
     2521 gctgtatcgc gtcgctgtcg cctccacccg gtcgccgacc cgtcgcaccg ccacccccag
     2581 cgatgcgaag tcatcgccca actcgtactg ggccaggtcg aactccaccc ttcccaatcc
     2641 agactccgta gacgtggacg gccccgcggg tgtcgccggc accggctggt ccgtgccacg
     2701 cagcatcccg gccagcggca tcgccacaac cccgatcgcc gcggccgccg ccaccaggct
     2761 gatcgccccg cgcaactgcc ggcgtccgcg caccctgcgg gcggacgcga tcacgtcgtc
     2821 cgttgccaca cccaggtcga ggtcacgcgt ccgcaagcca ttcaactcgg cgcgcagccg
     2881 ctcctccaga tcgatcatca ccgttctcct cccagtggcg cgtgcgccgc gcgcaaagtc
     2941 gccagcccgc gcgatgccgc ggacttcacc gtccccacac tgatcccgag gcactgcgcc
     3001 accgcagcct cggacaagtc ctcgaagtac cgcagcacga tcacccggcg ctgccgctcg
     3061 ggtagtccgg ccagcatccg cacgagctcg tcgcggtcgt cgatcacccg ctcggcatcc
     3121 cgcagatccg gacggtcgta gtcgtcggtc ggccgcgccg gacgccgccg ccaacggtcg
     3181 atgctcaagt tcaccagcgt ccgtcgggca taggccgcgg cctcaccatg ccgcaccctg
     3241 ggccaggcca gataggtttt gaccagcgcc tcctgcaaca cctcggccgc cgcgtccttc
     3301 tcgccggtca gcagataggc agtccacgac aacgacttcg acgccgagcg cacaaactcg
     3361 gtgaactccg cgtccctgcc gcgtttggaa gaacccaaca tctacgcgcc tttcgacctg
     3421 tgctcatcag gtcatacgca gatcgtggcc gtttggttgc tcgagctgaa cgcattcgct
     3481 ccagattccg cgacatcagg cccgaaagcg gccgccagcg ggatccgccg cgcaaatctg
     3541 ctcgcccgtg ccaagctgac tccatgcggg ttgcccttgc ccagctgatt gccagcaccg
     3601 atccagccgc caacctcaat caggtccgtg cgcacatcaa gcgtgcggtc gctgccggcg
     3661 ccgagctact cgtcctcccc gaagccacca tgtgttcgtt ctcgcggcca cgagccgaca
     3721 ccgccgagcc gttcgacgga ccttgggcga ccgccgtgcg cgcggccgcg gcggccgccg
     3781 gcctgaccgt ggtcgtcggc atgttcacca ccaccaacac cgcacgggtc gccaacacgc
     3841 tcctagctac gggcgttgtc gaagcccgct acgacaagat gcacctcttc gatgcccttg
     3901 gctacgccga
//
LOCUS       DAOPRQ010000131         3866 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_18850_length_3866_cov_12.773288, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000131 DAOPRQ010000000
VERSION     DAOPRQ010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 3866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3866
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18850_length_3866_cov_12.773288"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..777
                     /locus_tag="PKJ61_11910"
     CDS             <1..777
                     /locus_tag="PKJ61_11910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14568.1"
                     /translation="RVVGVKPVSVFDVSQTDGDPLPEAPRSELLTGQAPPGLWDALQG
                     FVEAQGYAVVRGDCGGANGLTAYDAREVRVRDDIDEAQAVKTLAHEAGHVLLHAPESR
                     EGFACRGVWEVEAESVAYLVTAAHGLDASQYTFNYVAGWAHDTATPDGPSVEDLVKAT
                     GQRVIGASDRILQATKPTPTLIDEALSDLAVEVGRGMVPSAADVEARVDLGPTAPSTV
                     QLPAWEFAGEQSSLPIDVPSAASPAQHPRRTPAGTVRRAL"
     gene            846..1439
                     /locus_tag="PKJ61_11915"
     CDS             846..1439
                     /locus_tag="PKJ61_11915"
                     /inference="COORDINATES: protein motif:HMM:NF017959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HOC14569.1"
                     /translation="MSYKGALRLLGLGVAALLLGIAPAFLAPGEGYSWIVQTTSESAA
                     QGASGAWVARLGFVTWGLTVLWFVADRSRLAQPVPRVALRMFGVLLMVVAACSHRHWL
                     PSVPFDPVEDQLHSIAASLMGFVFVLALLALALSRSRPKGLRLLNAVGAVASIVLPAL
                     MAVLPEWAGLLQRSMFAIAFVWFGVQIVSSERSSHVS"
     gene            1429..1986
                     /locus_tag="PKJ61_11920"
     CDS             1429..1986
                     /locus_tag="PKJ61_11920"
                     /inference="COORDINATES: protein motif:HMM:NF021886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOC14570.1"
                     /translation="MSLEHAILGFLHAQPQSGYDLKKNFDASVSHFWPADQSQIYRTL
                     NRLVADGWVTVEVIHQTERPDRKVYSLTGAGRDELLRWLVSELPGSPSRSAEMIQVFF
                     AAQLSDDQVLALFERVADGFRAGLAGYAAIPTDIEDHPGLTRSPREFYFAMLTLEIGT
                     MQLQANLDWIEDRIARIRAGEIPPS"
     gene            1983..2777
                     /locus_tag="PKJ61_11925"
     CDS             1983..2777
                     /locus_tag="PKJ61_11925"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOC14571.1"
                     /translation="MTRTPRQAPLRQRLRLALVLLSFLAFPLTLNFLSPYLIVVGAGQ
                     GVITGSFILFGVLFVASLFVGRLWCGWLCPGAGMAQVATWVNDAPFQAKRADRIKWVI
                     WTPWILLIAGLAFSAGGYRLVDPLFQTEGGISVSEPSRYVVYFAVLALFVGLAIAFGR
                     RAGCHTVCWMAPFMILGRSVRNRLGLPALQLVTTPETCSRCRTCTINCPMSLPVDELV
                     QTGRIEHPECILCTTCADGCRKGTIVLTVARPPVDRPGFSSSSAKP"
     gene            complement(2849..3403)
                     /locus_tag="PKJ61_11930"
     CDS             complement(2849..3403)
                     /locus_tag="PKJ61_11930"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOC14572.1"
                     /translation="MPKIRPYQPSDLESWLRCRVLSFLHTCYYDDVWQSRPDSPAIQL
                     IAADGETVIGLIDVEIVGSLATIETIAVHPDEQGRGIGSRILAEAERCLPRSVTLLDA
                     WTREDASTLAWYRARGFEESEQYLHVYKGSDDSNDDWSAPPPLHGPVMAFAHGLISDE
                     RELRSRFRRVYVCRRFSKAVREDR"
BASE COUNT          575 a         1202 c         1334 g          755 t
ORIGIN      
        1 cgggtggtgg gggtgaagcc ggtgtcggtg ttcgacgtgt cacagactga cggcgacccg
       61 ctgccggagg cgccgcggtc ggagttgctg accggtcagg ctccgcccgg tctgtgggac
      121 gccttgcaag gcttcgtcga ggcgcaggga tacgcggtgg tccgtgggga ctgtggcggg
      181 gcgaacgggc tcacggcgta cgacgcgcgg gaggtgcggg tccgggacga catcgatgag
      241 gcgcaggcgg tgaagacgtt ggcgcacgag gccgggcatg tgttgttgca tgccccggag
      301 tcgcgggagg ggttcgcgtg tcgtggcgtg tgggaggtgg aggccgagtc ggtcgcctat
      361 ctggtgacgg cggcgcacgg cctggatgcc tcgcagtaca cgttcaacta cgtcgccggc
      421 tgggcgcacg acactgccac cccggacggt ccgagcgtcg aggacctcgt gaaggccact
      481 gggcagcggg tgatcggcgc cagtgatcgg atcctgcaag ccacgaaacc aaccccaacc
      541 ctgatcgatg aagcactgag cgacctcgcg gtcgaggtcg gacgcggaat ggtcccatcg
      601 gctgcagatg tcgaggcaag ggtggacctt gggccgactg ctccctcaac tgttcagctt
      661 ccggcctggg agttcgcggg cgagcagtcg agtttgccga tcgatgtccc ttctgcggcc
      721 tcgcctgcgc aacatcctcg tagaactcca gctggcaccg tccggcgtgc gctgtgaaga
      781 actcgatgac ggacaattcg gccgctgctt gagctctgcc ctattggacc tgtaccttat
      841 gagatgtgtc ctataaaggt gcgctgagat tgctgggcct gggggtggcc gccttgctgc
      901 tcgggattgc ccctgcgttc ctggcgccgg gcgagggcta ctcgtggatc gtccagacga
      961 ccagcgagtc ggctgcccaa ggggccagtg gtgcctgggt agcccgtctc ggtttcgtga
     1021 cctgggggct gacggtcctg tggttcgtgg cggaccgcag ccgcctcgca cagccggtgc
     1081 cccgtgtggc gctgcggatg ttcggcgtcc tgctgatggt cgtggcggcg tgttcgcacc
     1141 ggcactggct cccctcggtg ccgttcgacc cggtcgagga tcagctgcac tcgatcgcgg
     1201 cgtccctgat ggggttcgtg ttcgttctgg ctttgttggc gctcgcgttg tcccgttctc
     1261 ggcccaaggg gctgcgcctg ctgaacgccg taggtgcggt ggcgtcgatc gttctcccgg
     1321 cgctgatggc ggtgctgccc gagtgggctg ggctgttgca gcggtcgatg ttcgcgatcg
     1381 cgttcgtgtg gttcggcgtc cagattgtgt cgtcggaaag gagttcgcat gtctcttgag
     1441 cacgccatct tgggattcct gcacgcccag ccgcagtcgg gctatgacct gaagaagaac
     1501 ttcgacgcgt ccgtgagcca cttctggccg gcggatcaga gccagatcta ccgcaccctg
     1561 aaccggctgg tcgctgacgg ttgggtgacc gtggaggtga tccaccagac tgagcggccg
     1621 gaccgcaagg tgtacagcct caccggtgca ggacgtgacg agttgctgcg ctggctggtc
     1681 agcgagctgc cgggctcgcc gagccgcagc gccgagatga tccaggtgtt cttcgccgcc
     1741 caactcagcg acgaccaagt gctggccctg ttcgaacggg tcgccgacgg attccgggcc
     1801 gggctggcgg gctacgcggc gatcccgacc gacatcgagg accaccctgg cctgacccgg
     1861 tcgccgcggg agttctactt cgcgatgctc accctcgaga tcggcacgat gcagctgcag
     1921 gccaacctgg actggatcga agaccgcatc gcccgcatcc gcgccggcga gatccccccg
     1981 tcatgaccag gacgccccgt caggcgccgc tgcggcagcg cctccggctc gcgttggtgt
     2041 tgttgtcgtt cctggccttc ccgctgacgc tgaacttcct gtcgccctac ctgatcgtgg
     2101 tcggggccgg ccagggcgtc atcacgggca gcttcatcct gttcggcgtc ctgttcgtgg
     2161 cgtcgttgtt cgtcggacgc ctctggtgcg gttggctgtg tcccggggcg ggcatggccc
     2221 aggtggcgac ctgggtcaac gacgccccgt tccaggcgaa gcgcgccgac cggatcaaat
     2281 gggtgatctg gacgccctgg atcctgctga tcgccgggct cgccttctcg gctggcgggt
     2341 accggctggt ggatccgctg ttccaaaccg agggcgggat ctccgtgagt gagccgtccc
     2401 gctatgtcgt gtacttcgcc gtcctggcgc tgttcgtggg gctcgcgatc gccttcggac
     2461 gccgcgccgg ctgccacacc gtgtgctgga tggcgccgtt catgatcctc ggacggagcg
     2521 tccgcaaccg actcgggcta cccgcgctgc agttggtcac cacccccgag acctgcagcc
     2581 gctgccgcac ctgcaccatc aactgcccga tgagtctgcc cgtcgacgaa ctcgtccaaa
     2641 ccggacgcat cgaacacccc gaatgcatcc tctgcaccac ctgcgccgac ggctgccgca
     2701 aaggcaccat cgtcctcacg gtcgcgagac cacccgtgga cagacccgga ttcagttcgt
     2761 cgtccgccaa gccgtgattg agcgcccgat tggaggtgga tgggcgaagt ctgcgacgcg
     2821 cgttggagcg gacctgatgg gggaacccct accggtcctc tcggacagcc ttgctgaacc
     2881 ttcggcagac gtacacgcgt cggaaacggc tccgcaattc ccgctcgtcg gagatcaacc
     2941 cgtgagcgaa ggccatcact gggccgtgca gtggaggtgg agcggaccaa tcgtcgttcg
     3001 agtcgtcgct gcccttatag acgtgcagat attgttcaga ctcttcaaat ccacgcgctc
     3061 gataccaggc aagggttgag gcatcctcgc gggtccaggc gtctagcaag gtcacgctgc
     3121 gagggagaca cctttctgcc tccgcgagga ttcgagaacc gatgccgcgg ccctgctcgt
     3181 cagggtgaac agcaatggtt tcgatggtcg cgagcgagcc cacgatctct acatcgatga
     3241 gcccaatcac ggtctcgcca tctgcggcga tgagttgaat tgctggactg tcggggcggg
     3301 actgccagac gtcgtcgtag tagcaggtat ggaggaacga taggacgcgg cacctcagcc
     3361 aggattcgag gtcgcttggc tgatagggac gaattttggg catggctgct cccggtgaga
     3421 actggatggg ttggagacac ctcaggaccg ggacatgctc ggcatgctag caaacgcggt
     3481 tgggaacggc cgcgactggc tttccgtcga ggcggctccc cacgttgtcg ccgccgattc
     3541 gtcagaagcg attgccccct gtgctcgcca tcatcggaca ggcgttggca gggtcctcgg
     3601 ctgtgagtct gggtgtggcc atccccggta ctttcgtacc gttttcgggg agcactgtcg
     3661 acattgggtc tggttcgact taccgtgcgg gaatccctgg tgggatgtct gaccgcttta
     3721 cctgtcccaa gttcatgggc ggggcttcgg cagaagtcgt ttcctcgttc ggcatcgcgt
     3781 ggcgctgtgg gcgtctgcgt acccctgtta gcccgactag cgcgcgttgc agccgcagga
     3841 gcggcgaatc accagggatg ccgggt
//
LOCUS       DAOPRQ010000132         3695 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_19992_length_3695_cov_15.066209, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000132 DAOPRQ010000000
VERSION     DAOPRQ010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 3695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3695
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19992_length_3695_cov_15.066209"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            98..424
                     /locus_tag="PKJ61_11935"
     CDS             98..424
                     /locus_tag="PKJ61_11935"
                     /inference="COORDINATES: protein motif:HMM:NF023257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3349 domain-containing protein"
                     /protein_id="HOC14573.1"
                     /translation="MSPTSDNPVRRVLRWLRAGRPDGVAPGDYVAVVAVLNRDLTEAQ
                     AEEVAQALLEEGPYDDELITFDEINDMVASKIHEQASQEDIEKVTALLAARGINLEAG
                     LPGVDA"
     gene            661..1917
                     /locus_tag="PKJ61_11940"
     CDS             661..1917
                     /locus_tag="PKJ61_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017934144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HOC14574.1"
                     /translation="MTTATTVILVMVVVTALAFDFTNGFHDTGNAMASSIATGALSPK
                     AAVTLAATLDLVGALISVKVALTITNAVIDGIQLKNGLPNPEYIGTENGKQGTLLLLV
                     VLAGLVGGIVWNLFTWLLGLPSSSSHALLGGLVGATIAGFGFGQVNWLGHSTEKLDGV
                     VGKVLLPSLISPVIAFIVAAVGTRIVFKLTERMGAKFRESRFRWGQVGSASLVALAHG
                     TNDAQKTMGVITLALIAAGDIDVTKEIGIPLWVKISAAVAIALGTYLGGWRIMRTLGK
                     GIVDINAPQGFAGDTSTASVILASSGLGFALSTTHVASGSIMGAGVGRKGAEVRWSVA
                     GRMASAWAITFPAAAVVGGLMLVIGRALPGLLGPSVVMIIVIGVSLLIWVRSRKDPVD
                     KHSVGKDWDAEPDAAPVVEAAHHVHH"
     gene            1946..2233
                     /locus_tag="PKJ61_11945"
     CDS             1946..2233
                     /locus_tag="PKJ61_11945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14575.1"
                     /translation="MNNFIIALDGAWKVLAAGLLLGAGIPTLYALAVNGLAMAQGGEA
                     SGKPANPVGKAIAAVVLVVILTVVAYGLAWLIFTSLGYSVSFSGILPTFKK"
     gene            2248..2574
                     /locus_tag="PKJ61_11950"
     CDS             2248..2574
                     /locus_tag="PKJ61_11950"
                     /inference="COORDINATES: protein motif:HMM:NF023257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3349 domain-containing protein"
                     /protein_id="HOC14576.1"
                     /translation="MSPHSEGPARRVLNWLRGGRPEGIAQGDYIALMGVLHRKLTTAE
                     VEHVATALLEEGYYDDDLITHDEIHDMIAAKVHEEATEEDIARVSELLAKAGIHLEAG
                     LPGADA"
     gene            complement(2694..2981)
                     /locus_tag="PKJ61_11955"
     CDS             complement(2694..2981)
                     /locus_tag="PKJ61_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008360832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3263 domain-containing protein"
                     /protein_id="HOC14577.1"
                     /translation="MADTAARLEAPGATLTERDREVLAFERHWWKYAGAKEQAIKELF
                     DMSATRYYQVLNGLLDNPAALEADPMLVKRLRRMRASRQRQRSARRLGLTL"
     gene            3261..>3695
                     /locus_tag="PKJ61_11960"
     CDS             3261..>3695
                     /locus_tag="PKJ61_11960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14578.1"
                     /translation="MTITPLDPQPGGGMPSGRVPGAAPAGEERVQPGVVGLSTAVVHA
                     TFDGIHAQLSAADGGVLVSSADIHEVDRIVRRAEALRLALVAKADQQQAHRQFGHSSM
                     SAWVAATTRSGGASAQRDVVLATALADGLDLTREAFHAGEVSV"
BASE COUNT          591 a         1273 c         1198 g          633 t
ORIGIN      
        1 cccacccggt ctggacacca tccgttcacc cgccccgggt accctgtgat ggaccgaggc
       61 cagcgcctcc ggttaccgag gaagtgaggt aatccccatg tcccccacct ccgacaaccc
      121 cgtccggcga gtgctcagat ggctgcgcgc cggtcggccg gatggggtag cccccggtga
      181 ctacgtcgcc gtcgtggcag tgctcaaccg cgacctgacc gaggcccaag ccgaagaggt
      241 cgcgcaagcg ctgctcgagg agggccccta cgacgacgag ctcatcacct tcgatgagat
      301 caacgacatg gttgcgtcga agatccacga gcaggccagc caagaggaca tcgagaaggt
      361 gaccgccctg ctggccgcga ggggtatcaa cctggaagcg ggcctgccag gagttgacgc
      421 ctgacacctg acgcgtgtcg tcgccgtcgg cgcggtcggc accgaacggc atacccaaag
      481 aagccgccca gactgaacct gggcggcttc tgcgtgccag cctgcggggt cttcgtggcc
      541 aatggcgaat atcaggtgaa cgcggcaagg cgcagaggcg agcggaaggt gccgtggttc
      601 acgtcctgac ataggacatt cgtccctcgg tcatctgccg ttcatcacca ccatggtgcg
      661 gtgaccaccg ctaccaccgt catccttgtc atggtcgtcg tcacggccct ggcatttgac
      721 ttcaccaatg ggttccacga caccggcaac gccatggcgt cttccatcgc caccggcgca
      781 ctgtctccca aggccgctgt gacgctcgcg gccactctcg acctcgtcgg agcgctcatc
      841 tccgtcaagg tcgccctcac catcaccaac gcggtcatcg acggcatcca gctgaagaac
      901 ggactgccca accccgagta catcgggaca gagaacggca aacagggcac cctgctgctc
      961 ctggtcgtgc tcgccggact ggtcggcggc atcgtctgga acctgttcac ctggctgctc
     1021 ggcctaccgt cgtcctcctc acacgcgctg ctcggcggcc tcgtgggagc cacgatcgcc
     1081 ggcttcggtt tcggtcaggt caactggctg ggccactcca ccgagaagct cgacggcgtg
     1141 gtcggcaagg tcttgttgcc ctcgctgatc tcccccgtca tcgccttcat cgtggccgcc
     1201 gtcggcaccc ggatcgtctt caaactcacc gaacggatgg gcgcgaagtt ccgcgagtcc
     1261 cgcttccgct ggggtcaggt cggctcggcc tcgctcgtgg ccctcgcgca cggcaccaac
     1321 gacgctcaga agaccatggg tgtcatcacc ctggcgctca tcgccgccgg cgacatcgac
     1381 gtgaccaagg agatcggcat cccgctgtgg gtcaagatct cggccgcggt ggccatcgct
     1441 ctgggcacct acctcggtgg ttggcgcatc atgcgcaccc tgggcaaggg catcgtcgac
     1501 atcaacgccc cgcagggctt cgccggcgac acctcgaccg cgtcggtcat cctcgcctcc
     1561 agcgggctgg gcttcgcgct ctcgacgacc cacgtcgcct ccggctcgat catgggcgcc
     1621 ggtgtcggtc gtaagggcgc cgaggtccgc tggtcggtcg ccggccggat ggccagcgcc
     1681 tgggcgatca ccttcccggc tgccgccgtc gtgggcgggc tcatgctcgt catcggccgc
     1741 gcgttgcctg ggctgctcgg cccgagcgtc gtcatgatca tcgtcatcgg cgtctcgctg
     1801 ctgatctggg tgcgctcgcg caaggacccg gtcgacaagc acagcgtcgg caaggactgg
     1861 gacgccgagc ctgacgcggc accggtcgtc gaagccgctc accacgtcca ccactgacgt
     1921 ccatcactga cccggaagag ttgacatgaa caacttcatc atcgccctgg acggcgcgtg
     1981 gaaggtcctg gccgctgggc tgctccttgg cgccggcata cccactctct acgccctggc
     2041 cgtcaacgga ctggccatgg cgcagggtgg cgaggcatcc ggcaagccgg ccaatccggt
     2101 cggcaaggcc atcgccgccg tcgtcctggt cgtcatcctg accgtggtgg cctacggcct
     2161 ggcctggctg atcttcacca gccttggcta ctccgtatcg ttcagcggca tcctgccgac
     2221 gttcaagaag tgaggtaggt ccccaccatg tccccccact ccgaaggccc cgctcgccga
     2281 gtcctcaact ggctccgagg cggtcggccc gaaggcatcg cgcagggcga ctacatcgcc
     2341 ttgatgggtg tcctgcaccg caagctgacg acggccgagg tcgaacatgt ggccaccgcc
     2401 ctgctcgaag agggctacta cgacgacgac ctcatcaccc atgacgagat ccacgacatg
     2461 atcgccgcca aggtccacga ggaagccacc gaggaggaca tcgctcgggt gtccgaactc
     2521 ctggctaaag ccggcatcca cctggaagcc ggactccccg gagccgacgc ctgacgcgac
     2581 ggtcaccgcg tcggcgcgcc gacgacgaag ccgcccagga gcacctgggc ggcttcgtgt
     2641 gtgtcgggga cggtatgccg tggcgtctca cggcgtggac gcggcgtggg cgcctacagg
     2701 gtcagaccca ggcggcgggc gctgcgctgc cgctgtcggc tggctcgcat ccggcgcagt
     2761 cgcttgacga gcatgggatc ggcctccagg gcagcggggt tgtcgagcag cccgttgagc
     2821 acctggtagt agcgcgtggc gctcatgtcg aacagttcct tgatggcctg ctccttggca
     2881 cccgcgtact tccaccagtg ccgctcgaac gcgagcacct cgcggtcgcg ctcggtcaag
     2941 gtcgcacccg gcgcctcgag ccgcgctgcc gtgtcagcca tcaatccctc catacccgca
     3001 tgtctgacag ccactatagg cggggaatga cacccatgtc attcgtcccc gtggcggctg
     3061 ggcaggccgt gacggctgtg acgaatgaga cgcatgaggt atgccgtgcg gtcacctcct
     3121 cggctccgcg gttggttggg cggcaccctc gcgacggttg gcgcgcccca tcagattgac
     3181 ccgccctctt cccaagaagt agttcaggaa aaacggactt tcatcttgtg ttagcacacc
     3241 tgtccgagta cgctagtttc atgacgatca cgccgctcga cccacagcca ggcggcggta
     3301 tgccgtccgg ccgggtcccg ggagcggcgc cggcgggtga ggagcgggtg cagccggggg
     3361 tggtggggct ttcgacggcg gtggtgcatg cgacgttcga tgggatccac gctcagctgt
     3421 ctgccgccga tggcggggtg ctggtctcgt cggcggacat ccacgaggtc gaccggatcg
     3481 tgcgccgggc cgaggcgctg cggttggcgt tggtcgccaa ggcagatcaa cagcaggcac
     3541 accgacagtt cggtcattcc tcgatgtcgg catgggtcgc tgcgacgacc cgtagtgggg
     3601 gtgcgagcgc gcaacgtgac gtcgtgctgg cgacggcatt ggcggacggg ttggacctca
     3661 cgcgtgaggc gttccacgct ggtgaggtct cggtg
//
LOCUS       DAOPRQ010000133         3641 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_20406_length_3641_cov_11.581428, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000133 DAOPRQ010000000
VERSION     DAOPRQ010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 3641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3641
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20406_length_3641_cov_11.581428"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(86..250)
                     /locus_tag="PKJ61_11965"
     CDS             complement(86..250)
                     /locus_tag="PKJ61_11965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14579.1"
                     /translation="MLPSGSANQTARIPPKVATSPSQLTFAAHPHLRLFPGNSIEVLE
                     CPPAPGGKED"
     gene            466..1209
                     /locus_tag="PKJ61_11970"
     CDS             466..1209
                     /locus_tag="PKJ61_11970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14580.1"
                     /translation="MIHILPMILMLAVLGGIAWFALAVAHGRIPLAKVVTAVALIFGI
                     GAAVVTALSLIDPLFYDRTTVAVPLVPFQPHPAPGITLEGMTATIVGGGVDRATLTLT
                     GLSWPAKSLIVSSWVLQGATWVAVCFVALRLARSLDQGDIFRDGTQALLRVAGIVAVS
                     GLLAAILADLGAWRAGTEALQHYGGAAEGAIAGLDPFDNLAQYGWPEPYGLLVNIPFW
                     PLTAGLGLALIAAAFKAGHKLREDTQGLV"
     gene            1209..1433
                     /locus_tag="PKJ61_11975"
     CDS             1209..1433
                     /locus_tag="PKJ61_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020578801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOC14581.1"
                     /translation="MPPEDEIGRIHCRLDELLAERGMTLTRLAELVGVSLVNLSILKN
                     DRARAIRFSTLTAICDVLECQVGDLLVVHR"
     gene            1587..3311
                     /locus_tag="PKJ61_11980"
     CDS             1587..3311
                     /locus_tag="PKJ61_11980"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOC14582.1"
                     /translation="MKGLARTLVLNGTVDPAAMSLAVEQFGESPELVNHLVQRRLATE
                     IEVAQAIADQTDHTFYDLAGVQLDPAVVALVPGHLCRKYQLIPIEMRPAEAYPMPRRV
                     ILAEAEEQSDELVLGMVDPTDIIAIDDVASITDTVIEPVVVTRSALAQAFEHFIRSDE
                     ELSEISAALEEDVAADTPIFTENLDGQASDAPVVRFVNLLIAQAVNDRASDIHIEPGE
                     HNLTVRFRIDGVLHEMQRADRAIQDGIISRLKIMSQIDIAERRRPQDGRISINHEGRQ
                     VDLRVATVPTVWGEKIVMRILDNKANSISMQDLGMSALNTDRFNRAITRPHGMILVTG
                     PTGSGKSTTLYASMGEVASPRVNVITIEDPVEFRIPGISQIQVNPKAGLTFSSALRAV
                     LRCDPDVVLVGEIRDQETAITSIEAALTGHLVFSTLHTNDAPSALTRLIEIGAEPYLV
                     GTALSVAVAQRLARRLCERCRTPIPYDPALLDRLEFPHDRSRPPSLYRANGCSVCAGT
                     GYRGRLALHEVMVVTEEIEQMVVRSATGGELRGLAIAQGMVPLRLDGFQKVAAGLTTI
                     EEVLRVSL"
     gene            3383..>3641
                     /locus_tag="PKJ61_11985"
     CDS             3383..>3641
                     /locus_tag="PKJ61_11985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14583.1"
                     /translation="MNQQVNRHPGADRDLVVTCGSRLETAELSTDAISTFAHPAQSHQ
                     THSMRSARPVILPTQPATKDILRSAGFTRAMIETQLRAGRLV"
BASE COUNT          635 a         1237 c         1157 g          612 t
ORIGIN      
        1 agcgcgtctc gttcgcaatg agaaggtcag gggttcgaat ccccttagct ccaccactaa
       61 gacccctagt caactagggg tcttttcagt cttccttgcc acccggagcc ggcgggcact
      121 ccagtacctc gatcgaattc ccggggaaga gccgcaggtg cgggtgtgcg gcgaacgtca
      181 gctgggacgg tgacgtcgcc accttcggcg ggatgcgggc ggtctggttc gccgatccgg
      241 acggcaacat catcaacctg gaaagcagtt cctaagcgcg gacgaagcga atcaccaggt
      301 tcttgatcgc gtggtggttg cgatagccgc agcgggtaat cgcaggtgat caggccacga
      361 gccatccatt ggccagtgag ttctggaaag ccctcctgga aacacttgcg gagcttatcg
      421 atagtcgtta tgttgtaatg actatcgata atccgaggag tcgtgatgat tcatatcctg
      481 ccgatgatcc tgatgctggc ggttctcgga ggcatcgctt ggttcgcgct cgcagtcgcc
      541 cacggtcgga tcccgttggc gaaggtggtc acggcggtcg cgctcatctt cgggatcggc
      601 gccgcggtcg tgactgcgct cagcctgatc gacccacttt tctacgaccg caccaccgtg
      661 gcggtcccac tcgtcccgtt ccaaccccat ccagccccag gaatcacgct cgaaggcatg
      721 accgccacca tcgtcggcgg cggcgtggat cgagccacgc tcaccctcac cggcctctct
      781 tggccggcca agtcgctcat cgtttccagt tgggtgctgc agggagcgac ctgggttgcg
      841 gtctgcttcg tcgcactccg gctcgcccgc tcactcgacc agggggacat ttttcgcgat
      901 ggcacccagg cactgctgcg agtcgcaggc atcgtcgcgg tcagcggact actggccgca
      961 atcctcgccg acctgggcgc atggcgggcc ggaaccgaag ccttgcagca ctacggcggt
     1021 gccgccgaag gagcgatcgc cggccttgac ccgttcgaca atctggctca gtacggttgg
     1081 ccggagccgt acgggcttct ggtcaacatt cccttctggc cgctcaccgc aggactggga
     1141 ctggccttga tcgctgccgc gttcaaagcc ggtcacaagc tccgcgagga cacccagggc
     1201 ctggtctgat gccaccagag gacgagatcg gacgcatcca ctgccgcctc gacgagctgc
     1261 tggcggaacg cggaatgact ctcacccggc tcgccgagct ggtcggggtg agcctggtga
     1321 acctttcgat cctgaagaac gaccgggccc gtgcgatccg gttttccacg ctcaccgcta
     1381 tctgtgacgt gctggaatgc caggtgggcg atctgctggt cgtccaccgc tgaacattcc
     1441 aggcctcaag ccgaggctga aggtgggatg gcgttccgcg tccgcagctt gacgctgctg
     1501 cgcagccgct cttggatgga ctgaacaggg cccgcggcca gcctgccaag gctacgcgcc
     1561 cacccggtca cgccaagggg tggtgcatga agggattggc acgaacgctc gtgctgaacg
     1621 gcaccgtgga ccccgcagcc atgtcgctgg ccgtcgagca gttcggcgag tcgcccgaac
     1681 tcgtgaatca cctggtgcag cggcgcctgg ccaccgagat cgaagtcgcc caggcgatcg
     1741 ctgaccagac cgaccacacg ttctacgacc ttgccggggt ccagctcgac cccgccgtgg
     1801 tggcgctcgt tcccgggcac ctctgccgga agtaccagct gatccccatc gagatgcgtc
     1861 cggccgaggc atacccgatg ccgcggcggg tgatcctggc agaagccgag gagcagagcg
     1921 acgagctcgt gctgggcatg gtcgacccga ccgacatcat cgccatcgac gatgtggcca
     1981 gcatcaccga cacggtgatc gagccggtcg tggtcacccg cagcgccctc gcccaggcct
     2041 tcgagcactt catccgctcg gacgaggaac tcagcgagat ctcggcagca ctggaagagg
     2101 acgtggctgc cgacaccccg atcttcaccg agaatctgga cgggcaggcc agcgacgccc
     2161 cagtggtccg gttcgtcaac ctgttgatcg cgcaggcggt gaacgaccgg gccagcgaca
     2221 tccacatcga gcccggcgag cacaatctca ccgtccgctt ccggatcgat ggcgtgctgc
     2281 acgagatgca gcgggccgac cgggccatcc aggacggcat catctcccgg ctgaagatca
     2341 tgtcgcagat cgacatcgcc gaacgccgcc gaccccagga cggtcgcatc tcgatcaacc
     2401 acgagggccg ccaggtcgac ctgcgcgtgg ccaccgtgcc gacggtgtgg ggcgagaaga
     2461 tcgtcatgcg catcctcgac aacaaggcca actcgatctc gatgcaggac ctgggcatgt
     2521 cggccctcaa cacggaccgg ttcaaccggg cgatcacccg tccgcacggc atgatcctgg
     2581 tcaccgggcc gaccgggtcg ggcaagtcga ccaccctgta cgcgtccatg ggcgaggtgg
     2641 ccagcccgcg ggtcaacgtg atcacgatcg aggacccggt cgagttccgc atccccggca
     2701 tcagccaaat ccaggtgaac ccgaaggccg gactgacctt ctcgtccgct ctgcgcgcgg
     2761 tgctgcgctg cgacccggac gtggtgctgg tcggcgagat ccgcgaccag gagaccgcga
     2821 tcacctcgat cgaggcggcc ctgaccggtc acctggtgtt ctcgaccctg cacacgaacg
     2881 acgcacccag cgcgctcacc cggctgatcg agatcggcgc cgagccctat ctggtgggca
     2941 ccgcgctgtc ggttgcggtg gcgcagcgcc tcgctcgccg gctctgcgaa cgctgccgga
     3001 cgccgatccc gtacgacccg gcgctgctcg accggctgga gttcccgcac gatcggagcc
     3061 ggccgccgtc gctgtatcgg gcgaacgggt gctcggtctg tgccggcacc ggataccgcg
     3121 gacgcctcgc gctgcacgag gtgatggtgg tcaccgagga gatcgagcaa atggtcgtgc
     3181 gcagcgccac cggaggcgag ctgcgcggcc tggccatcgc ccagggcatg gtgccgctgc
     3241 ggctggacgg cttccagaag gtggccgcgg gcctcaccac gatcgaagag gtcctgcgcg
     3301 tctcgctcta gcgatccgtc cgccaactgt aaccccggcg acggatgccg cccacactga
     3361 tcatcgccaa ggattcgtgg tcgtgaacca gcaggtcaac cgtcatccgg gagcagaccg
     3421 ggatctcgtc gtgacctgtg gatcaaggct tgagaccgcg gagttatcca cagacgcgat
     3481 cagcaccttc gcccatcccg cgcagagcca ccagactcat tcaatgcgaa gcgctcgtcc
     3541 ggtcatcctg ccgacccaac cggccaccaa ggacatcctc cggtcggccg ggttcactcg
     3601 cgccatgatc gagacccagc tcagggctgg ccggctggtg c
//
LOCUS       DAOPRQ010000134         3619 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_20565_length_3619_cov_12.134119, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000134 DAOPRQ010000000
VERSION     DAOPRQ010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 3619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3619
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20565_length_3619_cov_12.134119"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            99..1319
                     /locus_tag="PKJ61_11990"
     CDS             99..1319
                     /locus_tag="PKJ61_11990"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015071124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HOC14584.1"
                     /translation="MTTPDPDGSVLLTSSDVGALLVAAVQHAGGTLVTWALDHVDANP
                     AQSTTATYSALIDWPYGRREELLGVSARASGPTESDSAAEIFADGVREVAVWIYPQDP
                     DLPGLARAAYAESMAEVCNANKVFTNQVSPAELQLQMIGYRPRRRAVVRVSYGDAPQV
                     VYVKVLRARLFDDVLQRHNLLAAAGIPVAEVAATTSDHLLMLRELPGRPLSQAVFDAT
                     QPCTAEDLINLLDAMPSSVSRLDRRPPWSDAVGHYARIVAAALPALEAKLDRLVDTIT
                     SGLASVPLGSEPTHGDFHEGQVHVRDGRICGLLDTDTIGPGRRADDLACMVAHLSTIQ
                     RMSSAQEARVHELIRTWVPVFDTRVDPTELRLRAAAVIISLATGPFRGQEPEWERETA
                     KMIGSAEALVRQVS"
     gene            1423..3273
                     /gene="mutA"
                     /locus_tag="PKJ61_11995"
     CDS             1423..3273
                     /gene="mutA"
                     /locus_tag="PKJ61_11995"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015583101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase small subunit"
                     /protein_id="HOC14585.1"
                     /translation="MTDTPLTLAGDFASPTREDWEVEVLKVLNRRRPEGKELTIEQAM
                     KRLRTVTVDNLTIEPLYTDTDVPLGHPGVMPFTRGTMVKTSPMTSWDIRQLHEDPDVA
                     TTSREIIADLERGASSVWLRTGSDAIAVEDVAAVLADVDPNLAPISISSIEDQVAAAK
                     ALATFWKSADAKHARGNLGLDALALAARTGEAPDLSAQAQWVTAMLAELPGVRALTVD
                     VLPYDDAGASDVDQLAFAIATGVAYLRDLEAAGIAPGAAFGQIAFRVSANTDQFMTIA
                     RLRALRRLWARVGEASGVPAGARGAVQHAVTSWRMITRDDPWVNLLRGTIATFAAAVG
                     GAEIITCLPFDAAWGLPDEFSRRMARNTQALASEESNIGRVSDPAGGSWYVESLTDDL
                     AVKAWAAFQAIEAAGGMTSALTSGLVAERIGATTDERAKRLATRKLPLTGVSMFPKPD
                     EEPVQAKPRPAAPERHGLAPRRDAEIFEALRDRASAAEARPAVFLACLGERRDFGGRE
                     MFTGALLGVGGIDRPSSEGGTPEEIVAKVSEAGAKFVILCSSAKVYAREAVPVAQALK
                     DAGVPTVFIAGRKTETGAEDVDQVIDGEVFDGMDVVAFLNDAMDRIGVAK"
     gene            3270..>3619
                     /locus_tag="PKJ61_12000"
     CDS             3270..>3619
                     /locus_tag="PKJ61_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HOC14586.1"
                     /translation="MSQIPSFTDIELGAASVPEDSAEQFQALLEESISYSEGWQTPEH
                     ILVPPNFDASAYEGLDFLKTYPGIPPFLRGPYATMYVNQPWTIRQYAGFSTAEESNAF
                     YRRNLAAGQKGLSIA"
BASE COUNT          578 a         1265 c         1230 g          546 t
ORIGIN      
        1 tcggcgggcg aacgttgggc gccgagcgga tcgagcacta atctgctggc agcccggctg
       61 ggtacgggtg cagagtgtgc tgcgacaagg aggtgtgagt gaccacgccc gatcccgacg
      121 gctcggtgct gctcacctcg agtgacgtcg gcgccctgct ggtggcggcg gtgcaacacg
      181 ccggcggcac gctggtcacc tgggcgctcg accacgtgga cgcgaacccg gcacagtcca
      241 ccaccgcaac ctactccgcc ctgatcgact ggccctacgg ccggcgcgag gagttgctcg
      301 gcgttagcgc ccgtgcgtcc gggcccaccg agagcgattc ggcagcggag atcttcgccg
      361 acggggttcg cgaggtcgcg gtctggatct acccacagga tcccgatctg cctgggctgg
      421 cacgggctgc ctacgccgaa tcgatggccg aggtctgcaa cgccaacaag gtgttcacca
      481 accaggtgag ccccgccgaa ctgcagttgc agatgatcgg ctaccggccg cgtcggcggg
      541 ccgtggtcag ggtcagctac ggtgacgccc cgcaggtcgt gtacgtcaag gtactacgcg
      601 cgcggctctt cgacgacgtg ctgcagcgcc acaatctgct cgccgcagcc ggcattccgg
      661 tagccgaagt cgcggcgacg acgtccgacc acctcctgat gctgcgcgag ctgcctggtc
      721 gcccgctgtc ccaggcggtc ttcgacgcaa cccagccgtg cactgccgag gacctgatca
      781 acctgctgga cgcgatgccg tcgtcggtat cgaggctcga ccgccgtccg ccgtggtcgg
      841 atgcggtggg ccactacgcc cggatagtcg cggccgcact gccggcgctt gaggcaaagc
      901 tcgaccgcct cgtcgacacg atcacctccg ggctggcgtc cgtcccgctc gggagcgaac
      961 cgacccatgg cgacttccac gagggtcagg tgcatgtccg ggacggacgg atctgcgggc
     1021 tgctcgacac cgatacgatc ggccctgggc gccgtgccga cgacctggcg tgcatggtgg
     1081 cgcacctgtc cacgatccag cggatgagct cggcccagga ggcgcgggtg cacgaactga
     1141 tccgcacctg ggttcccgtc ttcgacacca gggtcgatcc caccgaactc aggctgcgtg
     1201 cagccgcggt gatcatctcc cttgccaccg gtccgttccg cggccaggag cccgaatggg
     1261 agcgcgagac cgccaagatg atcggctcgg ctgaggctct cgttcgccag gtgagctgag
     1321 cccgggcgga gcattcgtcc agcatgcggg cgctgggttg cgcgaacgtt ccgaaatggt
     1381 ccgtccgaca agccgccgtc cgacagcgat agtgtgaagc ccatgaccga cacgcccttg
     1441 acgcttgccg gtgacttcgc cagtcccacc agggaagatt gggaggttga ggtcctcaag
     1501 gtcctcaacc ggcggcgtcc cgaaggcaaa gaactgacca ttgagcaggc catgaagcgc
     1561 ctgcggacgg tgaccgtcga caacctgacg atcgagccgc tctacaccga caccgatgtc
     1621 ccgctggggc accccggcgt gatgccgttc acccgcggca cgatggtgaa gaccagcccg
     1681 atgaccagct gggacatccg ccagctgcac gaggatccgg acgtcgccac caccagccgc
     1741 gagatcatcg cagacctcga gcgcggcgcc agctctgtct ggctgcggac cggcagcgat
     1801 gcgatcgcgg tcgaggacgt cgccgcagtg ctggccgacg tagacccgaa cctcgccccg
     1861 atctcgatca gcagcatcga ggaccaggtg gcggcggcca aggcactggc caccttctgg
     1921 aagtcggcgg acgcgaagca cgcccgcggc aacctcggcc tcgacgccct cgcgctggct
     1981 gcccgcaccg gcgaagctcc cgacctgagc gcccaggcgc agtgggtgac ggcgatgctc
     2041 gccgagctcc ccggcgtccg cgcccttacc gtggacgtgt tgccgtatga cgatgccggc
     2101 gccagcgacg tggaccagct ggccttcgcc atcgccaccg gcgtggccta cctgcgcgac
     2161 ctcgaggcag ccggcatcgc gccaggtgcg gccttcggtc agatcgcgtt ccgggtaagc
     2221 gccaacaccg accagttcat gacgatcgcc cggctgcgcg cgctgcgtcg cctctgggcc
     2281 cgcgtgggcg aggccagcgg agtgccggcc ggcgctcgcg gggccgtcca gcacgcggtg
     2341 acctcctggc gcatgatcac ccgggacgac ccgtgggtga acctgctgcg cggcacgatc
     2401 gccaccttcg ccgcagccgt cggcggggcc gagatcatca cctgcctgcc gtttgacgcg
     2461 gcctgggggc tgccggacga gttcagccgc cgcatggcgc gcaacaccca ggcgctcgcg
     2521 tccgaggagt cgaacatcgg acgggtcagt gacccggccg gcggctcctg gtacgtggag
     2581 agcctgaccg acgacttggc cgtgaaggcc tgggccgctt tccaggccat cgaggccgcg
     2641 ggcggaatga cgtccgcgct cacgtccggc ctggtggccg agcggatcgg tgcgaccacc
     2701 gacgagcggg ccaagcgtct ggccacccgc aagctgccgc tcaccggcgt cagcatgttc
     2761 cccaagcccg atgaggagcc ggtgcaggcc aagccccgtc cggcagctcc ggagcgtcac
     2821 gggctcgccc cgcgtcgcga cgctgagatc ttcgaggcgc tgcgcgaccg tgcgtccgcg
     2881 gccgaggccc gtccggccgt gttcctcgcc tgcctgggcg agcgccgcga cttcggcggc
     2941 cgcgaaatgt tcaccggcgc gctgctgggc gtcggcggca tcgaccgtcc gtccagcgaa
     3001 ggcggtactc ctgaggagat cgtcgccaag gtgagcgagg ccggtgcgaa gttcgtgatt
     3061 ctctgctcct cggccaaggt ctacgcccgc gaggcggtgc cggtagccca ggcactgaag
     3121 gatgccggcg tgccgaccgt gttcatcgcc ggccgcaaga ccgaaaccgg tgccgaggac
     3181 gtcgatcagg ttatcgacgg cgaagtcttc gacggcatgg atgtggtcgc cttcctcaat
     3241 gacgccatgg accggattgg agtggcgaaa tgagccagat cccaagtttc accgacatcg
     3301 aactgggcgc agccagcgtt ccggaggact ccgcggagca gttccaggcg ttgctggagg
     3361 agtcgatcag ctacagcgaa ggttggcaga ccccggagca catcctggtg ccgccgaact
     3421 tcgacgcgtc cgcctacgag ggcctcgact tcctgaagac ctacccgggt atcccgccgt
     3481 tcctgcgcgg gccgtacgcc acgatgtacg tcaaccagcc gtggacgatc cggcagtacg
     3541 ccggcttctc caccgctgag gagtcgaatg ccttctaccg gcgcaacctc gcggccggcc
     3601 agaagggtct ttcgatcgc
//
LOCUS       DAOPRQ010000135         3518 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_21286_length_3518_cov_12.498989, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000135 DAOPRQ010000000
VERSION     DAOPRQ010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 3518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3518
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21286_length_3518_cov_12.498989"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            96..1094
                     /locus_tag="PKJ61_12005"
     CDS             96..1094
                     /locus_tag="PKJ61_12005"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HOC14587.1"
                     /translation="MADNDNYYLYGPRSTPLAQIDKASDQASYLHTDMLGSVRLSTDG
                     SAQVLGERTYTEFGSVLSAVGAIATVFGFTGNLTDPVTGLVHLRARDYDPETGQFVSV
                     DPALDDTRQPYAYASNNPVQFTDPTGLYTGDPVVDGIGGGLAYFAGLYDVITFGGFSA
                     FERWIDPGYDCFIETYKVSYDIGSGVGLFATIALGAASAGKALLSAFARDGGAAAVSA
                     GSVATASRFRSLTHAGSGIAPYSVQRAVTAGQGGSIQAHHLIEQRFADVMGQAIGEMP
                     SIVVTRAEHQVFTNAWREAIPYGSNGTGMATRAQVEGAARQIYSEYPEILRALGLG"
     gene            1253..1708
                     /locus_tag="PKJ61_12010"
     CDS             1253..1708
                     /locus_tag="PKJ61_12010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14588.1"
                     /translation="MKVEGAWVPNWRLDVICVEQGLAVEAAARFDVQTREVDWHPTSA
                     GAATQIIVPTVGPAWFDPEELREATIARHGVDGATCPRCGVWRWMPLTFGFLPPVRVT
                     QSLGDVAIAGSPEWFGDGKRAFRQVLVRRGLAELIAAASPRDFTVQDVL"
     gene            complement(1964..>2261)
                     /locus_tag="PKJ61_12015"
     CDS             complement(1964..>2261)
                     /locus_tag="PKJ61_12015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14589.1"
                     /translation="KVTVAKAKPKVTLKARYDRATGVATIAVRVKSSAATPTGYVKVS
                     LAGKSVRAELDASGRATVQLSDLGKSGKRTVTAWYAGSELLKSKTGKTSVKLKR"
     assembly_gap    2262..2361
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2362..>3518)
                     /locus_tag="PKJ61_12020"
     CDS             complement(<2362..>3518)
                     /locus_tag="PKJ61_12020"
                     /inference="COORDINATES: protein motif:HMM:NF027952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain repeat protein"
                     /protein_id="HOC14590.1"
                     /translation="IAPLEKLSKLSELNLASTGLASVHELAGLTGLTKLNLDGNHLRD
                     LSPLAALGSLSDLSATSQALTLAEVAGGVAVEVPSVTTVAGAKVTASLPDGAVASDSG
                     ASFRRAGTYRWPFASGDFTGAFSQVVTSDVAEPDAQAGASECVSAGKVWVVVERDTGL
                     QQGGCASKFGTGLEALVSAGFTTTGTSYVTAIDGYPSGSADSYWSYWHAANPVVDGSS
                     TRYSWSYSNVGANNYAPKAGSIEGWRFESWQTDAVAPSWTPVVRTAATPSPEPSPSTP
                     TTPAPEPATPAPTPGATATVATKVSAKAVKVTYGKRATIKVKVKPAKAKGMVTTVVGA
                     TTITAKLANGKAKLVLPARSLPPGKHSLTVSFADQGKYRASSKVVKVTVAK"
BASE COUNT          514 a         1091 c         1149 g          664 t
ORIGIN      
        1 atcagacgga ctcgcgaggc cttaggcagg tacgacagga cggcacgacc acggatcagt
       61 acctgtggtc gactgcgcgc acgttgcctc tgctcatggc tgacaacgac aactactact
      121 tgtacggccc gagatcgact ccgctggccc agatagacaa ggcatccgac caggcaagct
      181 atctgcacac tgacatgctc ggctcggttc ggctgagcac cgacggctcg gcgcaggttc
      241 tgggtgagcg aacctatacc gagttcggga gcgtgctcag tgctgttggt gcgatcgcca
      301 cggtgtttgg gttcaccggc aacttgaccg atccggtgac cggcttggtg caccttcggg
      361 cgcgtgacta cgatcctgag actgggcagt tcgtcagcgt cgatcctgcg cttgatgaca
      421 cgcggcagcc gtacgcttac gcctccaata atccggttca gttcacggat ccgaccggcc
      481 tgtacaccgg cgacccagtc gtggacggta ttggcggtgg gctggcctat ttcgctggcc
      541 tgtacgacgt gatcactttc ggcggcttca gtgcgttcga gcgatggatc gaccccggct
      601 atgactgttt catcgagacc tacaaggtct catacgacat cggctctggt gtgggtctgt
      661 tcgcgaccat cgcccttggt gcggcgagcg ccgggaaagc gctcctgtcc gcctttgcac
      721 gcgatggagg ggcagcggct gtttccgccg gaagtgtcgc cacagcgagc aggttcaggt
      781 ctctgaccca cgctgggtct gggattgcac cctacagtgt gcagcgggct gtgaccgctg
      841 gccagggcgg gagcatccag gctcaccatt tgattgagca gcgatttgct gacgtgatgg
      901 gccaagctat cggggaaatg ccgtcgatag tggtcacccg ggctgaacat caggtgttta
      961 cgaatgcatg gcgggaggcg attccgtacg ggtcgaacgg gacgggaatg gcgactcgtg
     1021 ctcaggtcga gggcgcggct cggcagatct acagtgagta cccggagatc cttcgagcgt
     1081 tggggttggg atgaccgatt tcgttcagtt gtactggaag cgcaatcgcg gttggccctg
     1141 gccggaggat tcgtggggtc tgacgccgat gtacggtccg gatggctggt gcgatacctg
     1201 cggggtgccg aagcactccc agtccggctc cctcgtgctt cagcgtaggg ggatgaaggt
     1261 ggagggtgcc tgggtgccga actggcggct ggacgtcatc tgtgtcgagc agggcctggc
     1321 tgtcgaggcg gcggcccgat tcgatgtcca gacccgcgag gttgactggc atcccacctc
     1381 agcgggcgcc gctactcaga tcatcgttcc caccgtcggc ccggcctggt tcgaccctga
     1441 ggagctccgt gaagcgacga tcgcgcgtca cggtgtcgat ggtgcgacgt gccccaggtg
     1501 cggcgtctgg cggtggatgc ccctcacttt cgggttcctg ccgccggttc gggtcacgca
     1561 atctcttggg gacgtcgcga ttgctgggag tcccgagtgg ttcggcgacg gcaagcgagc
     1621 cttccggcag gtcctggtac gacgtgggct tgcggaactg atcgctgcgg cgagtccacg
     1681 cgacttcacg gtccaggacg tcctttgatg gccggggtgt ttcttagtcg gttggccgcc
     1741 gacctggctg ccgtcctcat cacgcctttc gcgggtgttc tcggcacgga caggttccag
     1801 cgcccgctgc agccgctcct gttcagcgca agcgtcgagc gcggcctgca gttcgggggt
     1861 gatcacggca gcagcctagg tggcttgggc atcaaaatgc gggctgtgtg cgcctggtta
     1921 gggctgacaa cccgcatttc gatgcccgag cttgagcgga ggctcagcgc ttcagcttga
     1981 ccgaggtctt gccggtcttc gacttcagca gctccgagcc ggcgtaccag gccgtcactg
     2041 tgcgcttgcc cgacttaccc aggtcgctca actggacggt cgcgcgtccg ctggcatcaa
     2101 gttcggcgcg gacggacttc ccagccagcg acacctttac gtagccggtg ggcgtggctg
     2161 cgctcgactt gacccgcacc gcgatcgtcg ccaccccggt ggcacggtcg tagcgtgcct
     2221 tcagggtcac tttcggcttt gcctttgcga ccgtgacctt tnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn ncctttgcga ccgtgacctt tacgaccttg ctggatgccc
     2401 ggtacttgcc ctgatcggca aagctgacgg tgagcgagtg ctttcctggc ggcagcgacc
     2461 gcgccggcag caccagcttg gccttgccgt tggcgagctt ggccgtgatt gtggtggcgc
     2521 ccaccacggt cgtcaccatg cccttcgcct tggccggttt caccttcacc ttgatcgtgg
     2581 ctcgcttgcc gtaggtcacc ttgactgcct tcgccgagac cttggtcgcg accgtcgcgg
     2641 tggctcccgg tgtaggggca ggggtcgccg gttcgggcgc aggtgtcgtc ggcgtgctcg
     2701 gcgatggctc cggggacggc gtggcggcag tcctcaccac cggcgtccag gagggcgcca
     2761 cggcgtcggt ctgccacgac tcgaaccgcc agccctcgat cgaaccggcc ttcggcgcgt
     2821 agttgttcgc cccgacgttc gagtagctcc acgagtaccg cgtggacgag ccgtcgacga
     2881 ccgggttggc cgcatgccag tacgaccagt acgaatcagc ggacccgctc gggtagccgt
     2941 cgatagcggt cacgtagctg gtgccagtag tagtgaaccc ggcgctgacc agtgcctcca
     3001 gcccggtgcc gaacttgctc gcgcacccgc cctgctgcag cccggtgtcg cgctcgacca
     3061 caacccacac cttgccggcc gacacacact ccgacgcccc cgcctgtgcg tccggttcgg
     3121 ccacgtccga ggtgaccact tggctgaacg ccccagtgaa gtcgccggac gcgaacggcc
     3181 agcgatacgt cccggcgcgc cggaagctgg ccccactatc ggacgcgacc gccccgtccg
     3241 gcaggctagc ggtgaccttg gcgccggcga ccgtggtcac cgacggcacc tcgaccgcta
     3301 cgccgccggc cacctcggcc agcgtgagcg cctgggacgt cgcgctgagg tcgctgagcg
     3361 agcccaacgc cgccagcggg gagaggtcgc gcaggtggtt gccgtccaga ttgagcttgg
     3421 tcaacccggt cagcccggcc agctcgtgca ccgaagcgag tccagtcgag gccaggttca
     3481 gctcggacag cttgctgagc ttctccagcg gggcgatg
//
LOCUS       DAOPRQ010000136         3514 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_21318_length_3514_cov_11.843886, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000136 DAOPRQ010000000
VERSION     DAOPRQ010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 3514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3514
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21318_length_3514_cov_11.843886"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /locus_tag="PKJ61_12025"
     CDS             complement(<1..454)
                     /locus_tag="PKJ61_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016669853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOC14591.1"
                     /translation="MDEPAAAVGGSLSSAGHSDTPLAVRMRPRTVDEIVGQSHLLGPG
                     SPLRRLAEGRNAMSVFLWGPPGVGKTTIAAVVSATGQRRFVELSAVTAGVREVRQALA
                     EAQRQLARGVGTVLFVDEVHRFSKTQQDVLLPAVENRLVTLIAATTENP"
     gene            complement(491..1747)
                     /locus_tag="PKJ61_12030"
     CDS             complement(491..1747)
                     /locus_tag="PKJ61_12030"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOC14592.1"
                     /translation="MSSPNHPRLGSAFTNLWIATLASNLGDGIGRVAIPLLAARLTDD
                     PLLIAALGALAMLPWLLFAIPAGIVIDSADRRRVLASAQAVRVVVAVILLVLVATSAL
                     TIWWLLAVVFVYGIFETLYDGASRAIPASIVPTAELPRANGRIEAGEQVMQSFVSGPL
                     TSALFAVSVLIPLGLNAAAFAIAGVLALLLPAAASGRSAPAAGDDDPKVPWYRQFGDG
                     WRFLKGNRMLVTLWLTTTVVGIFVQLALASYVLFVLGPLGVPEAWFGVFMLSGAVGAI
                     IGSLSTASLKRRFNAGPVMAMSTLVSGVAILAMGLWPKVVVAAIANALLSASIVTWNV
                     LVMSLRQAIIPVALFGRVQGTWRTLLWGSMPLGSVLGGLVARVDLALPFLVGGGATTL
                     VGVIFFDFYRQLPNAEDVAVDVGAAV"
     gene            1844..2545
                     /locus_tag="PKJ61_12035"
     CDS             1844..2545
                     /locus_tag="PKJ61_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOC14593.1"
                     /translation="MNESRHDDEYFEPGEPRGRRRGGRRGHGHPGFGPGFGPGGPFGP
                     SFGPGGPFGPGGPFGAGGPMGPAPRGRGRRGHVRTSILALLAEQPLNGYQIMQTLAER
                     TDGAWKPSPGAVYPALSQLEDEGLIEAFDNDGQKAFRLTDAGRQAAEGVQTKPWEVVN
                     EALSGQRSDQIRALWQEFPTLAGAAKELTRSGTDAQLMEATALLAATRRKLYGLLAAE
                     EGTNSGDVHNGEDLR"
     gene            complement(2636..>3514)
                     /locus_tag="PKJ61_12040"
     CDS             complement(2636..>3514)
                     /locus_tag="PKJ61_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012921977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit C"
                     /protein_id="HOC14594.1"
                     /translation="QAEYVGGVVMPGGGSQPRRTFDAWQDWAKQRGAKGLAYVTIGED
                     GELGGPVAKNITEAERAGLAAAMDASPGDCIFFGAGRREATQELLGAARLEIARRCGL
                     IDESEWSFLWVVDAPMFKLREDAEASGDVAVAGGKYTAVHHAFTSPKPESMDTFDTDP
                     GSALSYGYDFICNGNEVGGGSIRIHRRDVQERVFAVMGLTPEESLEKFGFLLDAFAYG
                     APPHGGIAFGLDRMVMLLGGFETIRDVIAFPKSGGGFDPLTSAPAPISAKQRKEAGVD
                     YVPPAPKAQTPATPAT"
BASE COUNT          596 a         1254 c         1195 g          469 t
ORIGIN      
        1 acgggttctc ggtggtggcc gcgatcaagg tgaccaggcg gttctcgacc gcgggcagca
       61 gcacgtcctg ctgggtcttg gagaaccggt gcacttcgtc gacgaacagc acggtgccga
      121 caccacgggc cagttggcgt tgtgcctcgg ccagggcttg gcggacctcc cggacgccgg
      181 cggtgaccgc ggacagctcc acgaagcggc gttgcccggt ggccgagacc acagcggcga
      241 tcgtggtctt gcccacgccg ggcggacccc agaggaagac cgacatcgcg ttgcggccct
      301 cggccagccg gcgtagcggc gaacccgggc cgagcaggtg gctctggccg acgatctcgt
      361 cgaccgtgcg tggccgcatc cgcaccgcca gcggggtgtc gctgtgcccg gcgctgctca
      421 gcgacccacc gaccgctgcc gcgggctcgt cgatggggtt gccgaacaga tccgtactca
      481 cgcgccgacg ctacaccgca gcgccgacat cgaccgccac gtcctcggcg ttcggcaact
      541 ggcggtagaa gtcgaagaag atcacgccga ccagcgtggt cgcgccaccg ccgaccagga
      601 acggcaaagc cagatcgacc cgggccacca gcccaccgag gacgctgccc aacggcatcg
      661 agccccacag cagcgtccgc caggtgccct ggacgcggcc gaacagcgcg accgggatga
      721 tcgcctggcg cagcgacatc accagcacgt tccaggtcac gatcgaggcg ctgagcagtg
      781 cgttcgcgat cgcggctacg accaccttcg gccacagccc catggccagg atcgcgacgc
      841 cggagaccag ggtggacatc gccatcaccg ggccggcatt gaagcgtcgt ttcaggctgg
      901 cggtggacag cgagccgatg atcgcgccca cggccccgct cagcatgaac accccgaacc
      961 aggcctcggg caccccgagc ggcccgagga cgaagagcac gtagctcgcc agcgccagct
     1021 gtacgaagat gcccaccacc gtggtggtca gccagagcgt gaccagcatc cggttgccct
     1081 tcaggaagcg ccagccgtcc ccgaactggc ggtaccaggg caccttgggg tcgtcgtccc
     1141 cggccgcggg cgcgctgcgt ccagacgcgg ccgcgggcag cagcagcgcc agcaccccag
     1201 cgatcgcgaa ggcagccgcg ttcaggccaa gcggtatcag caccgatacc gcgaacagcg
     1261 cggacgtgag cggcccggag acgaagctct gcatcacctg ctcaccggcc tcgatccgtc
     1321 cattggctcg cggtagctcg gcggtcggca cgatgctcgc cgggatcgcg cgggacgcgc
     1381 cgtcgtagag cgtctcgaag atgccgtaga cgaagaccac cgcgagcagc caccaaatgg
     1441 tcagcgcgga ggtggccacc agcaccaaca ggatcaccgc caccaccact cgaaccgcct
     1501 gggcggacgc caggacgcgc cgccgatcgg cggagtcgat cacgatgcct gcggggatcg
     1561 cgaacagcag ccacggcagc atcgccaacg cacccaacgc agcaatcagg agcgggtcgt
     1621 cggtgagtcg tgcggccagc agcggaatcg cgacccgccc gatgccgtcg ccgaggttgc
     1681 tggccagcgt agcgatccac aggttggtga acgcggaacc gaggcgaggg tgattcggcg
     1741 aagacacccc agcaccctac gcctcgccgc ttgacgaatc ggccctgtcg acctactctc
     1801 gatatatcga cgaagttacg tcgatctgac aaggagaaca accatgaacg aatccagaca
     1861 cgatgatgag tacttcgagc cgggcgagcc gcgcggacgc cgccgcggtg gtcggcgcgg
     1921 ccacggccat ccgggcttcg gccccgggtt cggtccgggc ggtccgttcg gaccgagctt
     1981 cggcccaggt ggtccgttcg gtcccggtgg acccttcggc gccggtggcc caatggggcc
     2041 cgcacctcgc ggccgcggcc gtcggggaca cgtccggacg tccatcctgg cgctgctcgc
     2101 cgagcagccg ctgaacggct accagatcat gcagaccctg gctgagcgca ccgacggggc
     2161 ttggaagccc agcccgggcg cggtctaccc cgcactgtcg cagctggagg acgaaggcct
     2221 gatcgaggcg ttcgacaatg acggccagaa ggcctttcgg ctcaccgacg ccggccggca
     2281 ggctgccgag ggtgtgcaga ccaagccgtg ggaggtcgtc aacgaagccc tgtccgggca
     2341 gcgctccgac cagatccggg ccctctggca ggagttcccc accctcgccg gggccgccaa
     2401 ggaactcacc cgcagcggca ccgacgcgca actgatggaa gccaccgcgc tgctcgccgc
     2461 cacccggcgc aagctctacg gcctgctggc ggctgaggag ggcaccaact caggggacgt
     2521 ccacaacggc gaagacctgc gctagccctc gaccccttca caagctggac agttctggcc
     2581 cgtcactggc cgcaactgtc cagtttgcgc ttcctggggg cggtttgcgc tggggtcagg
     2641 tggctggggt tgcgggtgtc tgggccttcg gggccggtgg gacgtagtcg acgccggctt
     2701 ccttgcgctg cttggccgag atcggggcgg gcgcggacgt gagcgggtcg aagccgccac
     2761 ccgacttcgg gaaggcgatc acgtcgcgga tcgtctcgaa accgccgagc agcatcacca
     2821 tccggtccag gccgaaggcg atgccgccgt gcggcggggc gccgtaggcg aacgcgtcca
     2881 gcaggaagcc gaacttctcc agcgactcct cgggggtgag ccccatcacc gcgaagaccc
     2941 gctcctgcac gtcgcggcga tggatacgga tcgagccgcc gcccacctcg ttgccgttgc
     3001 agatgaagtc gtagccgtag ctgagcgcac tgcccgggtc ggtgtcgaag gtgtccatcg
     3061 actccggctt gggtgaggtg aacgcgtggt gcacagcggt gtacttgccg ccggccaccg
     3121 ccacatcacc ggacgcctcc gcgtcctcac gcagcttgaa catcggcgcg tccaccaccc
     3181 acaggaacga ccactcgctc tcgtcgatca gtccgcagcg gcgcgcgatc tccaggcggg
     3241 ccgcgccgag cagctcctgg gtggcctcgc ggcgtccggc accgaagaat atgcagtcgc
     3301 ccggagaagc gtccatagcg gcggccagcc cggcccgctc ggcctcggtg atgttcttgg
     3361 ctaccgggcc gccgagctcg ccgtcctcac cgatggtgac gtaggccagg cccttggcgc
     3421 cgcgctgctt ggcccagtcc tgccaggcgt cgaaggtgcg ccgcggctgc gaaccgccgc
     3481 cgggcatcac gacaccgccg acgtactcgg cctg
//
LOCUS       DAOPRQ010000137         3502 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_21421_length_3502_cov_13.418335, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000137 DAOPRQ010000000
VERSION     DAOPRQ010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 3502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3502
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21421_length_3502_cov_13.418335"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(201..1139)
                     /locus_tag="PKJ61_12045"
     CDS             complement(201..1139)
                     /locus_tag="PKJ61_12045"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HOC14595.1"
                     /translation="MGSRPTCWSWRPSYRELGGLCFGRSRPWADDSDWSWGPSAASTP
                     SSRFQEVRCPVATGELVTRHALESFLDRLRRTPHGKDFILKGGIVLAGYGIRRSTKDV
                     DSEAISASVTAAHIEQVVMDVAAVEVEDGLELDRSSLDVQTIRGAADYPGLRMRVHGT
                     VGTARVVLAWDISTGDPVVPLPTLVSIPRVLGEPIEMLGYAPETMVAEKGVTILERGI
                     TSTRWRDYVDIVALAQRGIDPDVLLASARAVARYRRVTLRPVAPSVAGYGATAQAKWA
                     AWRRKEGLEGMCKPDLDDQMREVIAILDPLFSRGPT"
     gene            complement(1051..1608)
                     /locus_tag="PKJ61_12050"
     CDS             complement(1051..1608)
                     /locus_tag="PKJ61_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14596.1"
                     /translation="MPAAELLRAGEHATSRSAFYRRAKVGAYERIARGVYLPSDADPA
                     DWDWLEATAKHPKATVCLASALAYYDLTDEIPQALDVAIPRGSRTPATDAAIAWHHFA
                     ADSFGLGRTTIAIPGSNVRIGIYGPERSIADAFRLRGSLGYELGRDALRSWLGRGGKP
                     ADLLELASQLPRTRGPLLRTIEALG"
     gene            complement(1804..2514)
                     /locus_tag="PKJ61_12055"
     CDS             complement(1804..2514)
                     /locus_tag="PKJ61_12055"
                     /inference="COORDINATES: protein motif:HMM:NF013488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HOC14597.1"
                     /translation="MSDAVDAVDAVDAVIAVDALDVQVWADLVCPWCYIAQRRLAAAV
                     AGYAGPVAVRHRAYELDPGMPSGERVPVAEYLGRKYGGGCAAGLAMTERVAAVAAEDG
                     LVLDFAQAVKSSTFDAHRLLALAAAEGAGQLAGELQEAMYAAHFRQGLALDDHGVLMD
                     LATGVGLSRNRIGKVLGSDAFAAQVRADQAEARSIGVTGVPFTVAGGRVAVSGAQPVE
                     VFAALLTQAADASGPPDR"
     gene            complement(2511..3443)
                     /locus_tag="PKJ61_12060"
     CDS             complement(2511..3443)
                     /locus_tag="PKJ61_12060"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOC14598.1"
                     /translation="METDALRWFQFVADGATVTEVAELHMVSQPSVSRALAALQDEIG
                     TPLLTRSGRVLRLTHAGSVFKRHVDRLINTLDDGLAAVDQLLDPERGTVVLAHDPSLG
                     SWLVPALIGGFRAEHPDVQFVVHQTGQEGVLASPDSLVESRVDLVITAQRPHGIPVQW
                     RLLFREDLFLAVPARHAWAGRTEVSMAEAAGEAFVVPRAPSPMRQRWERLVAAAGIEP
                     QVAFEVDDLTTARGFVAAGLGVAVVPATGDDPLPPSTGGPWLVRITDDAGARDVGLVW
                     STERALLPAAGLFRRYLLAHAPRPQASRSARLGR"
BASE COUNT          616 a         1259 c         1101 g          526 t
ORIGIN      
        1 tagagcaccg ctctcctaaa gcgggtgtcg cgcgttcgat tcgcgccggg ggcactctgc
       61 gcggcctgca tcgtggttcg ccgcatggtt cgctgacgtt ggccgcactc cactgagggt
      121 ccaatgtcac cggagtgcgg tgtccctcgc cgaagccgcc ggatcggcga tggctgagcg
      181 ccggagagac ctaccccgcg ctacgtgggc ccccttgaaa acaaggggtc caggatggcg
      241 atgacctcac gcatctggtc gtccaggtcc ggcttgcaca tgccctcgag gccttccttg
      301 cggcgccacg ccgcccactt ggcctgtgcc gtcgcaccgt acccagctac tgaaggcgcg
      361 acgggacgaa gggtaacccg gcggtatcgc gcgacagccc gcgctgaggc caagagcacg
      421 tctgggtcga tgcctcgttg agcgagcgca acgatgtcga cgtagtcgcg ccagcgggtg
      481 ctggtgatgc ctcgctcgag aatcgtgacg cccttctcgg ccaccatggt ctctggcgca
      541 tacccgagca tctcgatggg ctctcccagc actcggggaa tggacaccag ggtcggcagc
      601 gggacaaccg ggtcgccggt cgagatgtcc caggccagca cgactcgcgc ggttccaacg
      661 gtgccatgca cccgcatccg gaggccgggg tagtcagccg cccctcggat ggtttggaca
      721 tcgaggctgc tccgatcgag ttcaagaccg tcttcgacct cgacagctgc gacatccatc
      781 acgacttgct cgatatgcgc tgcggtcacg gaagccgaga tggcctccga gtccacatcc
      841 ttggtcgagc ggcgaattcc atagccagcc aacacgatgc cgcccttgag tatgaagtcc
      901 ttcccgtgcg gagtcctgcg aagtcggtct aggaacgact cgagagcgtg acgggtgacc
      961 aactcgccgg tcgcgaccgg gcatcggact tcctggaatc gtgagcttgg cgttgaagct
     1021 gccgaaggac cccatgacca gtcactgtca tcagcccaag gcctcgatcg tccgaagcag
     1081 aggcccccga gttcgcggta gctgggacgc caactccaac aagtcggccg gcttcccacc
     1141 cctacccagc caactgcgca acgcgtcacg gcccaactcg taccctaacg agccacgaag
     1201 ccggaacgcg tcggcgatgg accgctccgg accgtagatg ccgatgcgaa cgttcgagcc
     1261 agggatggcg atggtggtcc gacctaggcc gaaggagtcg gcggcaaagt ggtgccaggc
     1321 gatggcggcg tcagtcgccg gggtccggct acctcgtgga atcgcgacgt caagtgcctg
     1381 aggaatctcg tcggtcaggt cgtagtaggc cagcgccgag gccaagcaga cagtcgcctt
     1441 gggatgtttg gctgtcgcct cgagccagtc ccagtcagcg gggtcagcgt cagacggcag
     1501 gtacacgccg cgcgcgatcc tctcgtaggc gccgaccttc gcccgtcggt agaacgcgct
     1561 gcgggacgtg gcgtgctcac cagcacgaag cagttcggca gctggcatgc tgaccctcct
     1621 cgctggaaca aaacgtctac cgataattgt atctgtatac gatttgtttc gcaccttgcg
     1681 tggtctgtgg cggttcgccg cttggttcgc tgaggttcgc cgcactccac tgagggtcca
     1741 acctcaccgg agtacggctt ccctcgccga agcccccgat caaaccttcc ctgcatcgcc
     1801 cgatcagcga tcgggcggcc ccgacgcatc ggcagcttgg gtcaacaacg cggcgaagac
     1861 ctcgacgggt tgagcaccag aaaccgccac gcgtcccccg gccacggtga acggcacgcc
     1921 ggtcacgccg atcgagcgcg cctcggcctg gtcggcacgc acctgcgcgg cgaacgcatc
     1981 cgagccaagg accttgccaa ttcggttgcg agacaggccc acacctgtgg caaggtccat
     2041 cagcacaccg tgatcgtcca gcgccaggcc ctgccggaag tgtgcggcat acatcgcctc
     2101 ctgcaactcc ccggccagct gcccagcacc ctcggcagcg gccagcgcca agagccggtg
     2161 tgcatcgaag gtcgacgact tcacggcctg ggcgaagtcc aggaccagcc cgtcctcggc
     2221 agcaaccgcg gccacccgct cggtcatggc cagccctgcg gcgcacccgc cgccgtactt
     2281 gcgaccgagg tactcggcca ccggcacccg ctcgccggac ggcataccag ggtcgagttc
     2341 gtaggcccga tgccgcaccg ccaccggccc ggcataccca gccaccgccg ccgcgagccg
     2401 ccgctgcgcg atgtagcacc acgggcagac caggtcagcc cacacctgca cgtccagcgc
     2461 atccaccgca atcacggcgt caacagcatc cacggcatcc acggcatcgc tcatctcccg
     2521 agtctcgcac tcctggacgc ctgcggccgc ggcgcatgcg cgagcagata gcgccggaac
     2581 aggcccgctg cggggaggag agcgcgctcg gtcgaccaga ccagccccac gtcgcgggcc
     2641 ccggcatcgt cggtgatgcg caccaaccac ggcccgccgg tcgagggtgg cagcgggtca
     2701 tctccggtcg cgggcaccac agcaaccccc agcccggccg cgacgaaccc ccgcgcggtc
     2761 gtcagatcgt cgacctcgaa agccacctgc ggttcgatcc ccgcagccgc cacgagccgc
     2821 tcccaccgct gccgcatcgg gctcggcgcg cgcggcacga cgaacgcctc ccccgccgcc
     2881 tccgccatcg acacctcggt gcgccccgcc cacgcatggc gcgctggcac ggccaggaag
     2941 aggtcctcgc ggaagagtaa ccgccactgc accggtatgc cgtgcggccg ctgcgccgtg
     3001 atgaccaggt ccacccgact ctcgaccaag ctgtccggcg acgccagcac tccttcctga
     3061 ccggtctggt ggacgacgaa ctgcacgtcg gggtgctcgg cccggaagcc accgatgagt
     3121 gccggcacca accaggagcc caacgacggg tcatgggcga gcacgacggt cccccgctcg
     3181 gggtcgagca actggtcgac cgccgccagc ccatcgtcga gggtgttgat gagccggtcg
     3241 acgtggcgtt tgaagaccga ccccgcgtgt gtcagccgaa gcacgcggcc agaacgcgtc
     3301 agcagcgggg tgccgatctc atcctgcagg gccgccaggg cgcgcgagac gctcggctgg
     3361 ctcaccatgt ggagttcggc gacctcggtc accgtcgccc catcggcaac gaactggaac
     3421 caccgcagcg catccgtctc cacccggcga gtctatctat gcactatccg tatggataag
     3481 acatctcaga ggtattggac gt
//
LOCUS       DAOPRQ010000138         3384 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_22361_length_3384_cov_11.775608, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000138 DAOPRQ010000000
VERSION     DAOPRQ010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 3384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3384
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22361_length_3384_cov_11.775608"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="PKJ61_12065"
     CDS             <1..383
                     /locus_tag="PKJ61_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017932887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HOC14599.1"
                     /translation="GSLGSVIVFLLWLWLTNLALLFGAEIDAELERARQLQAGIRAER
                     TLQLPPRDDSGSLKAATKLEERVAEGRRLRLEAQASGKVEDSAIGYGRGHGHQRRRAR
                     SGEAPAHELEPLPSEIARARDRRP"
     gene            483..1607
                     /locus_tag="PKJ61_12070"
     CDS             483..1607
                     /locus_tag="PKJ61_12070"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOC14600.1"
                     /translation="MNRFHEGFVLGDRYTLTGRIASGGMGDVWEASDSVLERQVAVKI
                     LRPSLEEEEVFALRFRNEALHTANLAHVNVATVFDYGEEDNLAYLVMELVPGEALSDL
                     VRREGALPPDQVRAILAQAALALGAAHEAGVVHRDVKPANILVMPDGTVKLTDFGIAR
                     AVDGSGHTQTGEVLGTPHYLSPEQALGEAATGASDLYALGIVGHELLTGQRPFDKGTP
                     VATALAQVNDPPPPLPTHVPPGLRSLIEQCLEKKPSDRPASARELGERLGLPEADLPV
                     ASEDAAQVVGTAAAEEQVADERSAGEQPVGDQLAGDQPAGEPSGDAPTTTPRHWSGGD
                     PTPTPSSLPAVPKGHVHWAWVPGFSVALLAVLAAFYLFTR"
     gene            1737..1809
                     /locus_tag="PKJ61_12075"
     tRNA            1737..1809
                     /locus_tag="PKJ61_12075"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1770..1772,aa:Lys,seq:ttt)
     gene            2081..2371
                     /locus_tag="PKJ61_12080"
     CDS             2081..2371
                     /locus_tag="PKJ61_12080"
                     /inference="COORDINATES: protein motif:HMM:NF013677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOC14601.1"
                     /translation="MPETRKKYDREFREGAVRIVEETNKPIAAVARDLGVNEGTLGNW
                     VTRARAEREGRGEMSAGDIEELKRLRAENAELRMERDVLKRSVVLWVKEATK"
     gene            2368..3297
                     /locus_tag="PKJ61_12085"
     CDS             2368..3297
                     /locus_tag="PKJ61_12085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016666080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HOC14602.1"
                     /translation="MSVARFVVDQRTNYQVPVAFTCALLGISISWFYKWLPRTAIATG
                     LYTKRDLRRDTMDRAVKVMFDKNRGLHGSPRLVLDLREEGWTVSEKTVANSMRRQGLI
                     ARKIKRRNGLTKQDKSKAPFPDLIRRDFTATAVNQRWVGDITEIPTAAGKLYLATVID
                     LYSRRLIGAATSLHPNAELAKAAITMAVTVRGGKEAIWKEEESERVIFHSDRGSTYTA
                     DLFTRACRNLGIRQSMGRVGSCFDNAAAEAFFSSLEWEVLSRNEFRNTTEAATVVSDW
                     CWNFYNTDRRHSAASMMSPINYENAALTPRAAA"
BASE COUNT          563 a         1144 c         1157 g          520 t
ORIGIN      
        1 acggctccct cggaagcgtc atcgtcttcc tcctgtggct gtggctgacc aacctcgcac
       61 tgctcttcgg ggccgagatc gacgccgagc tggagcgggc acggcagctg caggcgggta
      121 tccgggccga gcgcacgctg cagctgccgc cgcgcgatga ctccggctcg ctcaaggccg
      181 ccacgaagct ggaggagcgc gtcgccgagg ggcgtcggct gcgcctcgag gcgcaggcgt
      241 cgggcaaggt tgaggactcc gcgatcgggt acggacgggg tcatggacac caacgacgca
      301 gggcccgctc cggtgaggca ccggcacacg agctggagcc cttgccgtcg gagatcgcgc
      361 gggcccggga ccggcgcccc tgagggagtc ctcggcctcc cggcacgtga cccgcgcgta
      421 cgcaggccgg tcaggggccc tgtggtgacc taccatggtc aggtgaatgc gaccaacccc
      481 cggtgaaccg gtttcacgag ggattcgtcc tcggcgaccg ctacacgctg acgggccgga
      541 tcgcgtccgg ggggatgggc gacgtctggg aagccagcga cagcgtgctc gagcgtcagg
      601 tggccgtgaa gatcctgcgg ccgagcctgg aggaggaaga ggtcttcgcg ctgcgctttc
      661 gcaacgaggc gctgcacacc gccaacctcg cgcacgtcaa cgtcgccacc gtcttcgact
      721 acggcgagga ggacaacctc gcctacctcg tcatggagct cgtgcccgga gaggccctgt
      781 cggacctcgt gcgacgcgag ggcgcccttc cgcccgacca ggtgcgcgcg atcctggcgc
      841 aggcggccct ggccctcggg gcggcccacg aggccggcgt cgtccaccgc gacgtcaagc
      901 ccgccaacat cctcgtcatg cccgacggca ccgtgaagct cacggacttc ggaatcgccc
      961 gggccgtcga cgggtcgggg cacacccaga ccggcgaggt gctcggcacc ccgcactacc
     1021 tctcccccga gcaggccctc ggcgaggcgg caaccggagc gtccgacctg tacgccctcg
     1081 ggatcgtggg ccacgagctg ctcaccggac agcggccctt cgacaagggg acgccggtcg
     1141 ccacggcact cgcccaggtc aacgacccgc ccccgccgct gccgacgcac gttcccccgg
     1201 gcctgcggtc gctcatcgaa cagtgcctcg agaagaagcc gtccgaccgg ccggcgagcg
     1261 cccgtgagct cggtgagcgt ctcgggctgc ccgaggccga cctgcccgtt gcctccgagg
     1321 acgccgcgca ggtcgtcggc accgccgcgg cggaggagca ggtcgccgac gagcgctcgg
     1381 ccggcgagca gccggtgggc gaccagctgg ccggcgacca gccggccggc gagccctcgg
     1441 gcgacgcccc gaccaccacc ccgcggcact ggtccggcgg cgaccccacc cccaccccgt
     1501 caagcctgcc ggccgtgccg aagggccacg tccactgggc ctgggtgccc gggttcagcg
     1561 tcgccctgct cgccgtcctg gccgcgttct acctgttcac gcgctgaacc gtggacgccc
     1621 ggtcccgggc cgggcggcgt gatccgggcc cggctacgcc atactgtgcg cggacgcgtg
     1681 cggccccgaa cctcggatgc cgtggcgcac ggtgcgtgga tgccgtctcg tcctccgggc
     1741 ctctagctca atggtcagag ctgcggactt ttaatccgta ggttgtgggt tcgagtccca
     1801 cggggcccac ccaaagtgtc ttacgggaac acgcgacatc gaaagatgtg gggtgttccc
     1861 gttcggcttc cttgccttcg tcgccgccgt cgtgcgcctc acggaagatc gttgcgaacg
     1921 gctccgcgag gtgtggccgt agttgttcgt cgtcggtgat gtcgaggcgt gaggtgtgtc
     1981 tgagtaaatg tcgggcgtag cgggataaat acccaaccag gatggtcgtg cgtttggact
     2041 tccccccgaa gcgtgtaggt atcagctata aggagatgct gtgcctgaga cacgtaagaa
     2101 gtacgaccgt gagttccgtg agggggctgt acggatcgtc gaggagacca acaagccgat
     2161 cgctgccgtc gcgcgcgatc tcggcgtcaa cgaagggacc ctgggcaact gggttacccg
     2221 ggctcgcgcc gagcgtgagg gacgcggcga aatgtcggcc ggtgacatcg aggagttgaa
     2281 gcggctacgc gcggagaacg ccgagctgcg gatggagcgt gatgtcctca agcgatccgt
     2341 ggtcctgtgg gtgaaggagg cgacgaagtg agcgtggcac gcttcgtggt cgaccagagg
     2401 accaactacc aggtgcccgt cgcgttcacc tgcgcgctgc tcgggatctc gatctcgtgg
     2461 ttctacaagt ggctacctcg gacggccatc gcgaccggcc tgtacaccaa gcgggacctg
     2521 cgccgcgaca ccatggaccg ggcggtgaag gtcatgttcg acaaaaaccg cgggttgcac
     2581 gggtccccgc ggctggtgct cgacctgcgc gaggaggggt ggacggtcag tgagaagacg
     2641 gtcgcgaact cgatgcgccg gcaagggttg atcgctcgga agatcaagcg ccgcaacgga
     2701 ttaacgaagc aagataagtc gaaggcgccg ttcccggact tgattcgccg ggacttcacc
     2761 gcgaccgcgg tcaaccagcg ctgggtcggt gatatcaccg agatcccgac cgcggccggg
     2821 aagctgtacc tggccaccgt gatcgacctg tactcccgcc gactgatcgg ggccgcgacg
     2881 tcgctgcacc cgaacgcgga actggccaag gccgcgatca ccatggccgt aacggtccgc
     2941 ggcggcaaag aggcgatctg gaaggaggag gaatcagagc gggtcatttt ccactctgac
     3001 cgcggctcga cctacaccgc ggatctgttc acccgcgcat gccgaaacct cgggatccgc
     3061 caatccatgg gacgcgtcgg atcgtgcttc gataatgcgg ccgcggaggc gttcttctcc
     3121 agcctcgagt gggaagtcct ctcccgcaac gagttccgaa acaccaccga agccgctacc
     3181 gtggtctcgg actggtgctg gaacttctac aacaccgacc gccgccacag cgccgcatcg
     3241 atgatgtcac ccatcaacta cgaaaacgct gccctcaccc cgagagcagc cgcctaagag
     3301 acaacctcca cgattcgggg ggaaccacag ttcgtggttg ttgttctcat tgtcctcgac
     3361 tcgctcggtg gtggtcgagg cgtt
//
LOCUS       DAOPRQ010000139         3362 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_22563_length_3362_cov_9.000907, whole genome shotgun sequence.
ACCESSION   DAOPRQ010000139 DAOPRQ010000000
VERSION     DAOPRQ010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 3362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3362
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22563_length_3362_cov_9.000907"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="PKJ61_12090"
     CDS             <1..807
                     /locus_tag="PKJ61_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012919338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HOC14603.1"
                     /translation="RRHSPPADGRLMIVAADHPARGALGVRGDANAMASRAELLERLA
                     VALTRPGVDGVLGTADILEDLLLAGLLEGKMVIGSMNRGGLQGAEFEFDDRWTGYDAE
                     TLAANGLDGGKMLTRICLEDEDTARVLESTGRAVTTLANHELMAMVEPFLSVRVAPDQ
                     VQNQLDADSVIRSIQIAAGLGATSAHTWLKLPVVAQMHRVLDSTTLPSLLLGGDPDGD
                     PDETYRRWADALTHPTAMGLVIGRALLYPADGDVATAVDTAVGLVHGGVA"
     gene            804..1712
                     /gene="iolB"
                     /locus_tag="PKJ61_12095"
     CDS             804..1712
                     /gene="iolB"
                     /locus_tag="PKJ61_12095"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HOC14604.1"
                     /translation="MSEWVLPAGTTADGDFDTRVLPVPSAGHGTSSGSGSEWAHTGLL
                     VTDLAAGESRTIDTGDLEYVVVPLAGSAQVTIGDQSYGLAGRTDVFEAATDVLYVPRD
                     ASFTITSTAGGRYAFCAATASRELPVRHLPASDVPDELRGAGTASRRVRNFGTPGVLE
                     ADSIIACEVITPAGNWSSYPPHKHDEEREGVETSLEEIYYFELRQEPGSPTSAENAFG
                     YQRVYGTAERPIDVLAEVRSRDVVLVPHGWHGPAMAPPGYDMYYLNVMAGPGATRAWL
                     ICDDPDHAWVRQTWADQRIDPRLTAN"
     gene            1824..>3362
                     /locus_tag="PKJ61_12100"
     CDS             1824..>3362
                     /locus_tag="PKJ61_12100"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102662 - malonate-semialdehyde
                     dehydrogenase (acetylating, NAD+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HOC14605.1"
                     /translation="MTITEQSSTTQSAATLKRLRYLVDNEWRESKTTSYMPVMNPSTG
                     EQIAEAPTCTQEEVDSAVAAAARAYPAWSATPLPQRIQLMFRFKQLLDEHLDELSVLL
                     ATEMGKVVAEARGDVLKAIEVVECACSTHYLMQGDTTMNISKGYDTVSYREPLGVFVG
                     IAPYNFPAMIPMGWMLPFAITTGNTFVLKAASMVPQTSMRMMELLIQAGLPKGVANLV
                     TCSRVEADSLLVHPDVRGITFVGSTAVGKHIYATAAGAGKRVQALTEAKNHALVLEDA
                     VLERSVAGIINSTYGCAGQRCMALPVIAVQESIADKVVEMLAAKAAELKLGPAYLPES
                     QLGPVITAKHREWVESCIQRGVDEGGDLVLDGRGVTVDGYEGGFYVGPTILDRVGPDN
                     WAGTHEIFGPVTSIKRVRDFEEGINIMNANPFANGSCIYTSSGHYAREFARRTHAGMV
                     GVNVGIPVPFSTFPFTGHKDSFFGDLHAMGKDGVAFFTETKAVTTTWFTEEHRHDKVS
                     TWDGM"
BASE COUNT          572 a         1141 c         1138 g          511 t
ORIGIN      
        1 cgccgccaca gcccgccagc tgacggccgg ctgatgatcg tcgccgccga ccaccccgcg
       61 cgcggtgccc tcggcgttcg gggggacgcg aacgcgatgg cgtcccgggc cgaactgctt
      121 gagcggctcg cggtcgcgct gacccgtcca ggggtggacg gggtgctggg taccgctgac
      181 atccttgagg acctgctgct ggccggtcta ctcgaaggca agatggtgat cggctcgatg
      241 aaccggggcg gcctgcaggg tgccgagttt gagttcgacg atcggtggac ggggtacgac
      301 gccgaaacgc tggcggccaa tgggctcgac gggggcaaga tgctcacccg gatctgcctt
      361 gaggacgagg acaccgcccg ggtcttggaa tccaccggac gcgcggtgac caccctggcc
      421 aaccacgaac tgatggcgat ggtcgagccc ttcctctcgg tccgggtggc tcccgaccag
      481 gtgcagaacc agttggacgc ggactcggtg atcaggtcga tccagattgc cgccggcctc
      541 ggcgcgacca gtgcacacac ctggttgaaa ctgccggtgg tggcgcagat gcaccgcgtc
      601 ctcgattcga cgacgctgcc cagcctgttg ctcggcggcg acccggacgg tgatccggat
      661 gagacctacc ggcggtgggc ggacgcgctg acgcacccca ctgcgatggg gctggtgatc
      721 gggagagcgc tgctctaccc ggcggacgga gatgtggcga cagcggtgga taccgccgtc
      781 ggcctggtac acggaggagt ggcatgagcg agtgggtact gcccgcaggg acgaccgcgg
      841 acggggactt cgacaccagg gttcttcccg tcccctcggc tgggcacggc acctcctcag
      901 gttcagggag cgagtgggcg cacaccggcc tgctggtgac cgacctggca gcgggggaat
      961 cgcgcacgat cgacaccggc gatctggagt acgttgtagt accgctggct ggtagcgccc
     1021 aggtgaccat cggcgaccaa agctacggac tggctggacg aaccgacgtg ttcgaagcgg
     1081 ccaccgacgt gctctacgtg ccgcgcgacg ccagcttcac gatcaccagc acagcaggcg
     1141 gtcgctacgc gttctgtgcc gcgaccgcgt cgcgggagtt gcccgtccgg catctcccgg
     1201 ccagcgatgt gcccgacgaa ctgcgtggtg cgggcaccgc cagccgccgc gtccgcaact
     1261 tcggcacccc cggcgtactg gaagccgact cgatcattgc gtgtgaggtg atcactcccg
     1321 ccggcaactg gtccagctac ccgccgcaca agcatgacga ggagcgcgag ggcgtggaga
     1381 cctcgctcga ggagatctac tacttcgaac tgcgccagga gccgggcagc ccgacgtctg
     1441 cggagaacgc cttcggctac cagcgggtct acggcaccgc cgagcgtccg atcgacgtgc
     1501 tggccgaggt gcggtcgcgc gacgtcgtgc tggtgccgca cggctggcac ggcccggcga
     1561 tggccccgcc cggctacgac atgtactacc tgaacgtgat ggccggcccc ggagcgacgc
     1621 gcgcctggct gatctgcgac gacccggacc acgcctgggt gcgccagacc tgggccgacc
     1681 agcggatcga cccgcgcctg accgcgaact gacctgaaac gactgaccca caactgaaca
     1741 ccgaatcacc cggcagcgtg gcccaaccag gctggcgctg ccgacaaacc gaccggatcg
     1801 gtcgacccag agaggatttt cgaatgacca tcaccgagca gtccagcacg acgcagagcg
     1861 cggccacctt gaagcgcctg cgctacctgg tcgacaacga gtggcgcgag tcgaagacca
     1921 cgtcctacat gccggtgatg aacccgtcca ccggcgagca gatcgccgag gcccccacct
     1981 gcacccagga agaggtcgac tccgccgtcg ccgctgccgc ccgcgcctac ccggcctggt
     2041 cggccacccc gctgccgcag cggatccagt tgatgttccg gttcaagcag ctgctcgacg
     2101 agcacctgga cgaactgagc gtgctgttgg cgaccgagat gggcaaggtc gtagctgagg
     2161 ctcgcggtga cgtgttgaag gcgatcgagg tggtggagtg cgcgtgttcg acccactacc
     2221 tgatgcaggg cgacaccacg atgaacatct cgaagggcta cgacacggtg tcctaccgcg
     2281 agccgctggg ggtgttcgtg ggcatcgcgc cgtacaactt cccggcgatg attccgatgg
     2341 gctggatgct gccgttcgcc atcaccaccg gcaacacctt cgtgctgaag gcggcctcga
     2401 tggtgccgca gacctcgatg cgcatgatgg agctgctgat ccaggccgga ctgcccaagg
     2461 gcgtggccaa cctggtcacc tgctcccgcg tcgaggccga cagcctgctc gtgcacccgg
     2521 acgtccgcgg catcactttc gtcggctcca ccgcggtggg caagcacatc tatgccactg
     2581 cggccggcgc cggcaagcgc gtccaggccc tcaccgaagc caagaatcac gcgctcgtgc
     2641 ttgaggacgc ggtgctggag cgctcggtgg cgggcatcat caactccacc tacggctgcg
     2701 ccggccagcg ttgcatggcg ctgccggtga tcgccgtcca ggaatcgatc gccgacaagg
     2761 tggtggagat gctggccgcg aaggctgctg agttgaagct cggcccggcc tacctgccgg
     2821 agtcccagct cggcccggtg atcaccgcca agcaccgcga gtgggtggag tcctgcatcc
     2881 agcgcggcgt ggacgagggt ggcgacctgg tgctggacgg ccgcggcgtc acggtggacg
     2941 gctacgaggg cggcttctac gtcggcccga cgatcctcga ccgggtcggt ccggacaact
     3001 gggccggcac ccatgagatc ttcggtccgg tgacgtcgat caagcgcgtc cgcgacttcg
     3061 aggagggcat caacatcatg aatgccaacc cgttcgctaa cggctcgtgc atctacacca
     3121 gctccggcca ctacgcccgc gagttcgccc gccgcaccca cgccggcatg gtgggcgtca
     3181 acgtgggcat cccggtgccg ttctccacct tcccgttcac cggccacaag gactcctttt
     3241 tcggcgacct gcacgccatg ggcaaggacg gggtcgcgtt cttcaccgag accaaggcgg
     3301 tcaccaccac ctggttcacc gaagagcacc gccatgacaa ggtcagcacc tgggacggca
     3361 tg
//
LOCUS       DAOPRQ010000140         3355 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_22621_length_3355_cov_10.906364, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000140 DAOPRQ010000000
VERSION     DAOPRQ010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 3355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3355
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22621_length_3355_cov_10.906364"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..175)
                     /gene="narY"
                     /locus_tag="PKJ61_12105"
     CDS             complement(<1..175)
                     /gene="narY"
                     /locus_tag="PKJ61_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002549337.1"
                     /note="with NarZV catalyzes the reduction of nitrate; the
                     beta subunit is an iron sulfur cluster containing electron
                     transfer subunit; one of 3 nitrate reductases in E. coli;
                     expression of nitrate reductase Z is not dependent on
                     nitrate levels; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HOC14606.1"
                     /translation="MARKLKRVMAHVGMVMNLDKCIGCHTCSVTCKQAWTNRAGTEYV
                     WFNNVETRPGLGYP"
     gene            complement(297..>3355)
                     /locus_tag="PKJ61_12110"
     CDS             complement(297..>3355)
                     /locus_tag="PKJ61_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002549317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HOC14607.1"
                     /translation="LIGGAMTSFYDWYADLPVASPQVFGDQTDVPESGDWWDSTYLMM
                     WGSNVPLTRTPDAHWMAEVRYRGTKVITVSPDYADNVKFADEWLPAQAGTDAALAMAM
                     GHVILKENFVERQVPFFADYVKQYTDLPMLVTLVEHPDGHGLTAAKFLTAEDLPRHTG
                     QTDAAFKTVLWDSVTDAPAVPNGTMGNRYNEDGQGKWNLDLGDLKPALSLLDVAGAEK
                     VEIALPCFEDPRGEGTIIRRGVPAVRVGGHLVTTVLDLMLAQYGVGRDGLPGTWATGY
                     DDVNSPYTPAWQAEITSVPADVCLRIAREFAKNSEDSKGRTMIIIGAGICHWFHADVT
                     YRAIMALLMLTGCIGRNGGGWAHYVGQEKCRPMTGWFNLANALDWSRPPRTMIGTGYW
                     YMHTDQWRTDGYSADRVKSPLSTGHLDGLHTADAMALSHRLGWMPFYPQFDRNPLDLA
                     DEAKAAVAAGEAESEGAWVASQLKEGKLTTALEDIDAPANWPRTMVVWRSNLFGSSAK
                     GNEYFLKHLLGTGSNLMPNNHAAEAKPKVVKFRDEAPEGKLDLLISADFRMTSTTLLS
                     DLVFPAATWYEKYDLSSTDMHPYVHAFTPAIDPPWESKSDFDFFTLLARAIGKLAKGR
                     LDTRADLVAVPMQHDTPGQISQPGGHVPDWRGTDLPAVPGKNLPALMVVERDYTAIAD
                     KLATVGPLADRLGFTVKNITYDVKGSVDLLGRLHGVMPSGAGAGRPAIDTDARLAEAI
                     LAFSGTTNGELATQGFHNLEAKTGRKLADLSEGQEERRITFKQTQEAPQPVITSPEWS
                     GSETGGRRYAAFTINTERLKPWHTLSGRMHFFLDHDWMIDAGEQLPTFRPPLDMTHAF
                     GEPELGPTGEKAITLRYLTVHNKWSIHSEYQDNLFMLSLGRGGPQAWLSVADAKSIGV
                     ADNDWIELTNANGVFVARAVVTHKLPDGICYVQHAQERTIDVPKSEATGKRGGIHNSV
                     TRILVKPTHLIGAYAHQSYAFNYLGPTGVQRDIVSTVRRRSQEVQY"
BASE COUNT          474 a         1068 c         1206 g          607 t
ORIGIN      
        1 gcgggtagcc gagcccgggc cgggtctcga cgttgttgaa ccagacgtac tcggttccgg
       61 cacggttcgt ccaggcctgc ttgcaggtga ccgagcaggt gtggcagccg atgcacttgt
      121 cgaggttcat caccatgccg acgtgcgcca tgacgcgctt cagcttgcgg gccattatcg
      181 ccgcctccct tgcgagagcc agcctccggc cccgagcgcc agccactgtg gctggcgctg
      241 gacggggatg gctggccctc gacgctgggg tcgcggaatc gatcggtgct ggttcatcag
      301 tactgcacct cctggctgcg gcggcggacg gtcgagacga tgtcgcgttg caccccggtc
      361 gggccgaggt agttgaacgc gtacgactgg tgtgcgtacg ccccgatcag gtgggtcggc
      421 ttgaccagga tccgggtcac cgagttgtgg atgccgccgc gcttgccggt ggcctccgac
      481 ttcgggacgt cgatcgtccg ctcctgggca tgctggacgt aacagatccc gtcaggcagc
      541 ttgtgggtca cgaccgcccg ggcgacgaac acgccgttgg cgttggtcaa ctcgatccag
      601 tcgttgtcgg ccacgccgat ggacttggcg tcggccacgc tgagccaggc ctggggacca
      661 cctcggccta gggagagcat gaacaggttg tcctggtatt cggagtggat cgaccacttg
      721 ttgtgcacgg tcaggtagcg cagggtgatc gccttctccc cggtcggccc gagttcgggc
      781 tcgccgaagg cgtgggtcat gtccagcggt ggacggaagg tcggcagttg ctcaccggcg
      841 tcgatcatcc agtcgtggtc gaggaagaag tgcatccgtc cggacagggt gtgccacggc
      901 ttgagccgct ccgtgttgat ggtgaacgcc gcgtagcgcc gtccgccggt ctccgaaccc
      961 gaccactccg gtgaggtgat caccggctgc ggggcctcct gggtctgctt gaacgtgatg
     1021 cggcgctcct cctggccttc gctgaggtcg gcgagcttgc gtccggtctt ggcctcgagg
     1081 ttgtggaagc cctgggtggc cagttcgcca ttggtggtgc cggagaaagc gaggatcgcc
     1141 tcggccaggc gggcgtcggt gtcgatcgcg ggacgacccg caccggcgcc ggacggcatc
     1201 acgccatgca gccgtccgag caggtcgacc gatcccttga cgtcgtaggt gatgttcttc
     1261 accgtgaagc cgagccggtc ggccagcggg cccaccgtgg cgagcttgtc ggcgatcgcg
     1321 gtgtagtcgc gttcgaccac catcagtgcg ggcaggttct tgcccggcac ggccggcagg
     1381 tcggtcccgc gccagtcggg cacgtggccg ccgggctggc tgatctggcc gggggtgtcg
     1441 tgctgcattg gcaccgcgac caggtcggcg cgggtgtcca ggcggccctt ggcgagcttg
     1501 ccgatcgccc tcgccagcag ggtgaagaag tcgaaatcgc tcttcgactc ccagggcggg
     1561 tcgatggccg gggtgaacgc gtgcacgtag gggtgcatgt cggtgctgga caggtcgtac
     1621 ttctcgtacc aggtggccgc ggggaagacc aggtcggaga gcagggtggt cgaggtcatt
     1681 cggaagtcgg cgctgatcag caggtcgagc ttcccctccg gggcctcgtc gcggaacttc
     1741 accaccttcg gtttggcctc cgccgcgtgg ttgttcggca tcaggttcga cccggtgccc
     1801 agcaggtgct tcaggaagta ctcgttgccc ttcgccgacg acccgaacag gttggagcgc
     1861 cagaccacca tggtgcgcgg ccagttggcg ggtgcgtcga tgtcctcgag cgcggtggtg
     1921 agcttgcctt ccttcagctg ggacgcgacc caggcacctt cggactccgc ctcgccagcg
     1981 gccaccgcag ccttggcctc atcggccagg tcgagcgggt tgcggtcgaa ctgcgggtag
     2041 aacggcatcc aacccagccg gtgcgatagc gccatcgcgt ccgcggtgtg caggccgtcc
     2101 aggtgcccgg tggacagcgg cgacttcacc cgatcggccg agtagccgtc ggtgcgccac
     2161 tggtcggtgt gcatgtacca gtagccggtg ccgatcatcg tccgtggcgg acgcgaccag
     2221 tcgagcgcgt tggccaggtt gaaccagccg gtcatcggcc ggcacttctc ctggccgacg
     2281 tagtgcgccc agccgccgcc gttgcgtccg atgcagccag tgagcatcag cagcgccatg
     2341 atcgcccggt aggtgacgtc ggcgtggaac cagtggcaga tgccggcgcc gatgatgatc
     2401 atcgtgcggc ccttggagtc ctcggagttc tttgcgaact cgcgggcgat ccgcaggcac
     2461 acgtccgccg gcaccgacgt gatctcggcc tgccaggccg gggtgtaggg cgagttgacg
     2521 tcgtcgtagc cggttgccca ggtgcccggc agcccgtcgc ggccgacgcc gtactgggcc
     2581 agcatcaggt cgaggaccgt ggtgaccagg tgcccgccca cgcggacggc cgggacgccg
     2641 cggcggatga tcgtgccttc tccgcgcggg tcctcgaagc agggcagcgc gatctcgacc
     2701 ttttcggcac cggcgacgtc gagcaggctg agcgcaggct tcaggtcacc caggtcgagg
     2761 ttccacttgc cctggccgtc ctcgttgtag cggttgccca tggtgccgtt cgggacggcc
     2821 ggcgcgtcgg tgacgctgtc ccacagcacg gtcttgaagg cagcgtcggt ctggccggtg
     2881 tgccgcggca ggtcttcggc ggtcaggaac ttggccgcgg tcaggccgtg tccgtccggg
     2941 tgctcgacga gggtgaccag catcggcagg tcggtgtact gcttcacgta gtcggcgaag
     3001 aacggcacct ggcgctcgac gaagttctcc ttcaggatca catggcccat cgccatggcc
     3061 aaggcggcgt ccgtgccggc ctgggcgggc agccattcgt ccgcgaactt cacgttgtcg
     3121 gcgtagtcgg ggctgaccgt gatcaccttg gtgccgcggt agcgcacttc ggccatccag
     3181 tgcgcgtccg gggtgcgggt caacgggacg ttcgagcccc acatcatcag gtaggtcgaa
     3241 tcccaccagt cgccggactc agggacgtcg gtctggtcgc cgaacacctg cggggaagcg
     3301 accggcaggt cggcgtacca gtcgtagaag gacgtcatcg cgccaccgat gaggt
//
LOCUS       DAOPRQ010000141         3333 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_22818_length_3333_cov_11.215985, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000141 DAOPRQ010000000
VERSION     DAOPRQ010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 3333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3333
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22818_length_3333_cov_11.215985"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..901)
                     /locus_tag="PKJ61_12115"
     CDS             complement(<1..901)
                     /locus_tag="PKJ61_12115"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757242.1"
                     /note="catalyzes the reduction of ribonucleotides to
                     deoxyribonucleotides; the rate-limiting step in dNTP
                     synthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HOC14608.1"
                     /translation="MTETAKATGRGARKASGLKIARVFTTAGVHPYDEVKWELRDVVQ
                     TNWKTGEAVFEQRGVEFPDFWSVNASTIVTTKYFRGALGSAQREASLKTLIDRVVKTY
                     RAGGEDNGYFASEEDAEIFEHELTWMLLNQYFSFNSPVWFNVGTLSPQQVSACFILSV
                     DDSMDSILNWYKEEGFIFKGGSGAGLNLSRIRSSKELLSSGGTASGPVSFMRGADASA
                     GTIKSGGATRRAAKMVVLDVDHPDIEEFIETKAREEDKIRALRDAGFDMDLGGKDIVS
                     VQYQNANNSVRVSDEFMQAVEDGT"
     gene            complement(944..1402)
                     /gene="nrdR"
                     /locus_tag="PKJ61_12120"
     CDS             complement(944..1402)
                     /gene="nrdR"
                     /locus_tag="PKJ61_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007078015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HOC14609.1"
                     /translation="MHCPFCRHTDTRVLDSRVAEDGTSIRRRRQCPACERRFTTLEQM
                     QLVVVKRSGVVEPFSREKVISGVAKACKGRPVSETDLARLGQRVEETLRASGQAEIPA
                     DEVGLAILGPLRELDAVAYLRFASVYKHYDSVDDFEAEIESLRQAALVTT"
     gene            complement(1629..1943)
                     /locus_tag="PKJ61_12125"
     CDS             complement(1629..1943)
                     /locus_tag="PKJ61_12125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14610.1"
                     /translation="MSALLVDDLVPQVATSANGWHHVRGREYGRLALVPDLEDGVGSA
                     PVVTNRRAGWRLTDRGIKVVVSFFLALFATALVVLVSGFFAVSDAPISVPQAPAAVAV
                     AG"
     gene            2145..2909
                     /gene="lexA"
                     /locus_tag="PKJ61_12130"
     CDS             2145..2909
                     /gene="lexA"
                     /locus_tag="PKJ61_12130"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HOC14611.1"
                     /translation="MATKADPPARKRGRPRREDVAAQLGKKVSQLPDTPVDEEGLTLR
                     QRRVLEVIRESLIEVGYPPSVREIAARSGLGSASSAAHQLKQLQQKGFLYRDPHRPRA
                     LEVRLPESMSMPAPVTRIVEPDLPAPTAPSVPVPILGRIAAGGPILAEQAVEDVFALP
                     KQLVGEGTLFLLEVKGDSMIEAAICDGDYVVVRQQPDAVNGDIVAALLGDEATVKTFK
                     RTPGQVWLLPHNPAYEPIDGNEASILGKVVAVLRRV"
     gene            2816..3184
                     /locus_tag="PKJ61_12135"
     CDS             2816..3184
                     /locus_tag="PKJ61_12135"
                     /inference="COORDINATES: protein motif:HMM:NF014633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HOC14612.1"
                     /translation="MAAPPQPGLRADRRERGVDPRQGRRCPAARLVRRLAIHIAIRVK
                     PGAGRTRVGGRYGDATLIVAVSAPAVDGRATEAALTTLAAALGCKAREVSLVSGATSR
                     TKVVEVPDATRPRYLELLDS"
BASE COUNT          569 a         1135 c         1057 g          572 t
ORIGIN      
        1 cggtgccgtc ctcgaccgcc tgcatgaact cgtcgctgac ccgcaccgag ttgttggcgt
       61 tctggtactg gacgctgacg atgtccttgc cgcccagatc catgtcgaag ccggcatcgc
      121 gcagcgcgcg gatcttgtcc tcctcgcgcg ccttggtctc gatgaactcc tcgatgtcgg
      181 gatgatccac gtccagcacg accatcttcg ccgcgcgccg ggtggcgccg ccggacttga
      241 tcgtgcccgc ggacgcgtcc gcaccgcgca tgaacgacac cgggccggac gcggtgccac
      301 cggaggacag caactccttc gacgaccgga tccgggacag gttcaggccg gcgccggagc
      361 cgcccttgaa gatgaaaccc tcttccttgt accagttgag gatcgagtcc atcgagtcgt
      421 cgacgctcag gatgaagcac gcgctgacct gctgcgggct gagcgttccg acgttgaacc
      481 agaccgggga gttgaagctg aagtactggt tcagcagcat ccaggtgagt tcgtgctcga
      541 agatttcggc gtcctcttcg gacgcgaagt agccgttgtc ctcaccgccg gcccggtagg
      601 tcttgaccac ccggtcgatc aacgtcttca ggctcgcctc gcgttgggcg ctgccgagcg
      661 cgccccggaa gtatttcgtg gtgacgatcg tggacgcgtt cacgctccag aagtcgggga
      721 actcgacccc ccgctgctcg aacaccgcct caccggtctt ccagttcgtc tgcacgacgt
      781 cgcgcaactc ccacttgacc tcgtcgtagg ggtggacgcc ggcggtcgtg aaaacccggg
      841 cgatcttcag ccccgatgcc ttccgggcac cccggccggt tgccttcgcg gtttcggtca
      901 tggttgtgct gcctttcgat tcttcttcgt ggggttgatt tgctcaggtg gtgacgagcg
      961 ctgcctggcg caggctctcg atctcggctt cgaaatcgtc gaccgagtcg tagtgcttgt
     1021 agacggatgc gaaccgtaga taggcgaccg cgtcgagttc gcgcagcggg ccgaggatgg
     1081 cgaggccgac ctcatcggca gggatctcag cctggccgct ggcacgcagg gtctcctcca
     1141 cccgctgccc gagccgggca aggtcggttt ccgagaccgg gcgacccttg caggccttgg
     1201 ccacgccgct gatcaccttc tccctggaaa agggctccac cacgccggac cgcttcacca
     1261 ccaccagttg catctgctca agagtggtga agcgacgctc gcacgccggg cactgccgac
     1321 gccgtcggat gctcgtgccg tcctcggcca cacggctgtc cagcaccctc gtatcggtgt
     1381 gccggcagaa cgggcaatgc atctccgggc cccttcctgg ggatcgcgcg gtggatatcg
     1441 ctgtggacga ggtgtggacc gcaaccacaa tctgtggacg aaccacaacg ctgtaactac
     1501 tagatgtaga ggttacgacc gaatgccgtc actatcaaga agaccactcc ggcgtgtcgg
     1561 cgcaggcggt gacaattggt gacgccggct ggtcatggcg accagccggc gtcgggaact
     1621 actaccggtc agcccgcaac cgcgaccgcc gcgggcgcct gcggcactga gattggcgca
     1681 tcgctcaccg cgaagaagcc gctgaccagc accaccagcg cggtggcgaa caacgccagg
     1741 aagaagctca cgacgacctt gatcccacgg tcggtgagcc gccagccggc gcggcggttg
     1801 gtgacgaccg gtgcgctgcc gaccccatcc tccagatcgg gcaccagagc gagccggccg
     1861 tactcgcgtc cccggacgtg gtgccaaccg ttcgcggacg tggctacctg cgggaccagg
     1921 tcatctacca gcagggcact catggcttct cctcgtgtct cgaacaggtg ttcgaatcat
     1981 ttctacccca ccagatcacc caggtcaagg gattcgaacg tctgttctgc cgtgtttctt
     2041 cgtcgagtta catgctggac ggcgcctctg acagttttcg gagaacccac gacacgctag
     2101 aacacatgtt ccaatagcga tgcgccacgc gctagctttg acacatggca accaaggcag
     2161 atcccccggc ccgcaagcgc ggccggccgc gtagggaaga cgtagccgcg caactgggca
     2221 agaaggtcag ccaactgccc gacaccccgg tcgacgaaga agggctgacc cttcggcagc
     2281 gcagggtgct ggaagtcatc cgcgaatccc tgatcgaggt cggctatccg cccagcgtcc
     2341 gtgagatcgc ggcgcgttca ggtttgggtt cggcatcatc ggccgcgcac cagctcaagc
     2401 agctgcagca gaagggcttc ctctaccgcg acccgcaccg tccgcgcgcc cttgaggtac
     2461 gcctgcccga gtcgatgtcg atgccggctc cggtcacgcg catcgtcgag cccgacctgc
     2521 ccgcgcccac cgcaccttca gtcccggtac cgatcctcgg tcggatcgcc gccggcggcc
     2581 cgatcctggc cgagcaggcc gttgaggacg tattcgcgct gcccaagcag ttggtgggtg
     2641 agggcaccct gttcctcctt gaggtcaagg gcgattcgat gatcgaggcg gcgatctgtg
     2701 acggagacta cgtggtggtg cggcagcagc ccgacgcagt caacggcgac atcgtggctg
     2761 ccctgctcgg cgacgaggcc accgtgaaga ccttcaagcg cacccccggc caggtgtggc
     2821 tgctccccca caacccggcc tacgagccga tcgacgggaa cgaggcgtcg atcctcggca
     2881 aggtcgtcgc tgtcctgcgg cgcgtctagt tcgcaggctc gccattcaca tcgcgatccg
     2941 ggtcaagccc ggagccggac ggaccagggt cgggggacgc tacggcgacg ccaccctgat
     3001 cgtcgccgtc tcggcaccag ccgtggacgg acgcgccacc gaagccgctt tgacgacgct
     3061 ggcggcggct ctgggctgta aggcgcggga ggtgtcgctg gtgagtgggg ccaccagtcg
     3121 caccaaggtc gtcgaggtgc cggacgcgac gcgtccgcgc tacttagagt tgctggacag
     3181 ctgaagcttc gcccgaatag acccgggcct gagtcgccag acggtggatc cccctaccaa
     3241 caaagtggac cgttcctcgc cccgacgccg ccagaccgtc cactcggtga agcggggacg
     3301 caccgccgac ccgcccgccc gccaacaaag tgg
//
LOCUS       DAOPRQ010000142         3299 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_23114_length_3299_cov_13.104192, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000142 DAOPRQ010000000
VERSION     DAOPRQ010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23114_length_3299_cov_13.104192"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..72)
                     /locus_tag="PKJ61_12140"
     tRNA            complement(2..72)
                     /locus_tag="PKJ61_12140"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38..40),aa:Gly,seq:tcc)
     gene            285..1592
                     /gene="tig"
                     /locus_tag="PKJ61_12145"
                     /pseudo
     CDS             285..1592
                     /gene="tig"
                     /locus_tag="PKJ61_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002523196.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
     gene            2019..2909
                     /locus_tag="PKJ61_12150"
     CDS             2019..2909
                     /locus_tag="PKJ61_12150"
                     /inference="COORDINATES: protein motif:HMM:NF016370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HOC14613.1"
                     /translation="MRIDDLLANGDGLVRLRDHPAQARTVQRAAKAGKVRSLLPGIYV
                     TADQTLSAGLRLRAACAWSTSGVIHGHTAVEVYLNRPISLPIRLRAPYVGVTPVKWLK
                     VSWGTVPSRREYSGVRVASPSHCAVELAATDDGEVIFDMLRRGLVTPDSLAEVLPQFV
                     GTPGNRRRTRAVTLAADNPWSFAEARLHRLLRDAGIGGWVANRPILVRGRWIAPDVRF
                     EREHLVLEFDGEAVHSTHDQFEDDRSRQNVLVLGGFRVLRITWRMLVEEPDAVLVMVR
                     IALAQESVAISQGKGRSQAN"
     gene            3053..>3299
                     /locus_tag="PKJ61_12155"
     CDS             3053..>3299
                     /locus_tag="PKJ61_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002527077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HOC14614.1"
                     /translation="MAAGPGQVGMADSVYQSLLANRIIFLGSEVRDDNANAICAQMLL
                     LNAEDPVKDIYLYINSPGGSVDSGMAIYDTMQWISNDV"
BASE COUNT          572 a         1078 c         1113 g          536 t
ORIGIN      
        1 gagcggatga cgggaatcga acccgcatgg ccagcttgga aggctggggc tctgccattg
       61 agctacatcc gcgcagcggt ttcgcgtccg cgcgcagcaa agcctaacgg aatcgagccg
      121 ctggccagaa tcgggcctcc tcgtaacgcc cggctgagct tgacgatgcc tcgggtggtc
      181 cctcgtcaag caggcggatc ctggcggccc gtcggctggc tcgactgcct tcggttgtcc
      241 actaggattg cccagtctgt ctactttccg atcaggagca tgccttgccc accaccgtcg
      301 agaagctcag cccttcacgg gtcaagctca ccgttgagat ccccttcgcc gacctcaagc
      361 ctcacctgga caaggcgtac cgcgagatcg cccagcaggt cacgattccc ggcttccgca
      421 agggcaaggt gcccagcatg gtgatcgacc agcgtttcgg ccgcggcatg gtcctgcagg
      481 aggcgatcaa cgcggctctg cccggcgctt tcgaggcagc catcgtcgag tctgaagtcg
      541 tgccgctggg cgagccttcg gtggagatca ccaagctgga ggacggcgac gtggtcgagt
      601 tcaccgccga ggtggacgtc cggcccgact tcgaggtgcc cgacttcgcc accctgaggg
      661 ccaccgtcga cgccaagcgc gacgtcgccg ccgaggtcga ggagcggatc gaactcctcc
      721 gtacccgttt cgcgaccacc accgaggtcg accgcgccgc ggccgagggc gatcaggtca
      781 ccatctcgct ggtcggcagc cgcgacggtg agcagttggc ggacgccacc gctgagggcg
      841 tcacctacgt gatcggctcc ggtggcatga tcgacggcct cgacgaggcg gttaccgggc
      901 tgaaagcggg cgagtccgcc gagttcgcgt ccaccctggt cggcggcgcg ctggagggcg
      961 agcccgcaga catcaccgtc accgtgagca aggtgagcga gcagaccctg cccgaggtgg
     1021 acgatgagtt cgcgtccatg atctccgagt tcgacaccgt cgaggagatg caggccgacc
     1081 tcacccaggg cgtcgaggag tacctcaccg cagagcaggt ctccgccggt cgcgacaagg
     1141 tgctcgaagc cctggttgag gccaccgact tcgaggtgcc tgctggcgtc ctggaagccg
     1201 agatcggctc ccgcaaggag cagatcaacg agcagctctc ccgcgccgga ctcacccttg
     1261 agcgctacct ggagcaggcc gaggaagaag ccaacacgcc ggaggagttc tgggcagagg
     1321 tcgagaagtc cacgctccgt gggctcaagg cgcagctgat cctggacaag gtggccgacg
     1381 acgctgcggt gaacgtcgag caggccgatc tctccgaact gctggtgcgc aaggctgccc
     1441 agaacggctc gacgccggag caggaagccc agcacatgat ggagcacaac cacatcggcg
     1501 cctggatgca ggagatccgc cgcaacaagg cgctcgcgct gatcgtggcc aaggccaagg
     1561 tcgttgacac cgagggcaac accgtcgacg tggccgtccc cgagccggca gctgaggcca
     1621 ccgaggccga gtaagccgaa gcaaccgcga tcgccgtcac ctctccggtg gcggcgatcg
     1681 ttgcgtctcg gcgcgcctgc gctcacttgc atagcgccgt tgtcgcgatc tcgtggctgt
     1741 ttgccaagaa tcgggcggtt cgcgacgtct gactcgcttc cgcttcgaaa tcgcgacaca
     1801 gtcgccctgg acaaggctgg gacggggtcg gcgtgccggc gcttcagtgc gctcgagcgt
     1861 gtgcacaacg cgcacacctt agtgtcgcaa tctcgtagcg ggacacccag ctgacggcgc
     1921 cgtccgcact gtccacagaa tctgaccggc agatagcgac atccacaggc cggggtttcc
     1981 cgtgacgcgg atcgatggcg gctgccgata agtagggcat gaggattgac gacctgctcg
     2041 ccaatggcga cggcttggtg cggctgcgtg accatccagc gcaggctcgc actgtgcagc
     2101 gggccgcgaa ggcgggcaag gtgcggtcgc tgctaccagg gatctacgtg acggcggacc
     2161 agaccctgtc ggctgggttg cggcttcggg cggcttgtgc ctggtcgacc tcgggtgtga
     2221 tccatgggca caccgcggtc gaggtctacc tgaatcgtcc gatcagcctg cccattcggc
     2281 tgcgcgcgcc ctatgtcggt gtcaccccgg tgaagtggct gaaggtcagt tggggcacgg
     2341 tgccgagtcg gcgcgaatac tcgggcgtac gggtggcatc gccctcgcac tgcgcggtcg
     2401 aactcgctgc gaccgacgac ggcgaggtca ttttcgacat gctgcggcgt gggttggtga
     2461 cgcccgattc gctagccgag gtcctgccgc agttcgtcgg cacgccgggc aaccggcgac
     2521 ggacccgggc ggtcactttg gccgcggaca acccgtggtc cttcgcggag gcgcggctgc
     2581 acagacttct tcgcgacgcc ggcatcggcg gctgggtggc gaaccgtcca atcctggtgc
     2641 gtggccgctg gatcgcgccg gacgtccggt tcgaacggga gcacctcgtc ctggagttcg
     2701 atggcgaggc ggtgcacagt acgcacgatc agttcgagga cgaccgtagc cggcagaacg
     2761 tgctcgtgct cggcggcttc cgagtgctcc gaatcacctg gcggatgctg gtcgaggaac
     2821 ccgatgcggt gctggtcatg gtgcggatcg cgctcgctca ggagtctgtc gcgatctcgc
     2881 agggcaaggg ccgatcacag gcgaactgag gcctcgggcc gtatccggct gcgaattcgc
     2941 ggcacgcggt gcgccgacag cgaacaggcg tatcaacccc accgaaaaga gggtgcaacc
     3001 gggtaggttc gcctgcgtga acgaatatcg agccaaccag accggcccct tcatggccgc
     3061 cggtcccggc caggtaggca tggccgactc ggtctaccag tccctgctgg cgaaccggat
     3121 catcttcctc ggttccgagg tgcgcgacga caacgcgaac gccatctgcg cgcagatgct
     3181 gctgctcaac gccgaagacc cggtcaagga catctacctc tacatcaact ccccgggcgg
     3241 gtccgtcgac tccggcatgg cgatctacga caccatgcag tggatctcca acgacgtcg
//
LOCUS       DAOPRQ010000143         3264 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_23472_length_3264_cov_11.712060, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000143 DAOPRQ010000000
VERSION     DAOPRQ010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23472_length_3264_cov_11.712060"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2022
                     /locus_tag="PKJ61_12160"
     CDS             <1..2022
                     /locus_tag="PKJ61_12160"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOC14615.1"
                     /translation="LEKSAVDARAAAGRYGIAEVTEKQTARTLAPLSGLGHFLLADRC
                     WPGTRRAQVDLVVVGPGGVFIVDTKAWAEVRIDNGRIYRGDADVTDDLLGLADLADVT
                     QADLAEVGLAPGEVRPIIALAGRKDIDATLNGVRVVGERDVLKCIASHGNRLTPAQVD
                     IVLSRALALFPQANAPAPIVATVPEPVLPMPAGEVAQEALLSPEEVNDALLQGILAQP
                     IEEWMSFLHPQQAKLVRRSFPGPSRVRGPAGTGKTVVGLHRAAHLARTRPGRILVTTY
                     VRTLPDVLGELLRRLAPEVVDRVEFTGTHAFARRVLQDRGVHFKLDPSASRDAFDVAW
                     SQVGAKSVLGNGTKDHDYWADEIEHVIKGRGITQFEHYADLTRTGRRHPLNLHGRQAV
                     WELYCAYDAELRKRGINDFADLVLLAEAELRRKPMVGVYSAVIVDEAQDLSCAMIRLL
                     HSLVGDEPDGFTLIGDGQQSIYPGGYTLAEAGISVAGRGVVLDINYRNTAQIVNFARR
                     LVAGDEYADIEGVLALGDVPSSVPRAGAEPVIARCANRRELNATMISRVRSVTKDIGT
                     GIGDVAVLCITRRSAMDATAALLAAGFPVINLEEYGGVPLDAVKVGTIKRAKGLEFKQ
                     VLIPDIRREHTITEPPTDETDRERWDLTRRELYVAMTRARDGLWVSISN"
     gene            2058..2519
                     /locus_tag="PKJ61_12165"
     CDS             2058..2519
                     /locus_tag="PKJ61_12165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14616.1"
                     /translation="MPVFTDIRSRSTRDLLAAYVEILDELLRRKVIRTRNAPAGDLAE
                     RVVAEAYSGELASNSLKSWDVRASDGRLLQVKSRVIGADTSRSQVFSPFRTFDFDTCV
                     FVIFDARDYSISNAIELPAESVRGLSHRSEWVAGNRVRVFADLLNAPAPST"
     gene            complement(2797..3123)
                     /locus_tag="PKJ61_12170"
     CDS             complement(2797..3123)
                     /locus_tag="PKJ61_12170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14617.1"
                     /translation="MGISQVQIDQFLTSYAAALTELDAEAGAELWSMPGVIADDRFTV
                     VIETRAAMVTGLKQSYPLYQKLGLGSVTYELLEAKPLTEKLVLAHVHWNFLDTHGDLS
                     TAPPTT"
BASE COUNT          557 a         1059 c         1054 g          594 t
ORIGIN      
        1 ctagagaagt ccgctgtcga cgcccgcgca gccgccgggc gttacggaat cgcggaggtc
       61 accgagaagc agacggcgcg aacgctcgct ccgctctcgg ggctgggcca cttcctgctc
      121 gcagatcgat gctggccggg aactcgccgc gcccaggtgg atttggtcgt ggtcggtcct
      181 ggcggagtgt tcatcgtcga caccaaggcc tgggccgagg tccgaatcga caatggccgg
      241 atctatcgcg gcgacgccga cgtgaccgac gacttgctcg ggctagctga tctcgcggac
      301 gtcacccagg ccgacctcgc agaggtcggc ctcgccccgg gagaagtgcg tccgatcatc
      361 gccctggccg gccgcaagga catcgacgcc accctgaacg gggtccgggt cgtcggcgag
      421 cgggacgtgc tgaagtgcat cgccagccat ggcaaccggc tcacacccgc ccaggtcgac
      481 atcgttctat cgcgcgctct cgccctgttt ccgcaggcca acgcccctgc cccgatcgtg
      541 gcaacagtgc ccgagccggt gctgccgatg cccgctggcg aagtggcgca ggaggccctg
      601 ctcagtcctg aggaggtgaa cgatgctctt ctccagggga tactcgccca gcccatcgag
      661 gagtggatgt ccttccttca tccacagcag gccaagctcg tccgacgctc gttccctgga
      721 ccgtcgcgcg ttcgtggacc ggccggcacc ggcaagaccg tcgtcgggct ccaccgggct
      781 gcgcatctcg cgcgaaccag gcccggacgc atcctggtca ccacctatgt gcgcaccctc
      841 ccggatgtcc tgggtgaact gctgcgccgg ctcgcgcccg aggttgtcga ccgcgtcgag
      901 ttcacgggaa cgcacgcgtt cgctcgccgc gtcctccagg atcgcggcgt ccacttcaag
      961 ctcgatccgt cagcgagccg agatgcgttc gacgtcgcct ggagccaggt cggcgccaaa
     1021 tctgtcctcg gcaacggcac caaggaccac gactactggg ccgacgagat cgagcatgtg
     1081 atcaaggggc gcggcatcac gcagttcgag cactacgccg atctcaccag gaccgggcgc
     1141 cggcatccgc tcaacctcca tggccgccaa gcggtttggg agctgtactg cgcctacgac
     1201 gctgaactgc gcaagcgcgg catcaacgac ttcgctgatc tggtgttgct cgccgaagct
     1261 gaactccgcc gtaagccaat ggtcggcgtc tactccgctg tgatcgtgga cgaggcgcag
     1321 gacttgtcct gcgcgatgat ccgcctactg cactccctag tcggcgacga accggacggc
     1381 ttcaccctca tcggggacgg ccaacagtcc atctaccccg gcggctacac cctcgccgaa
     1441 gccgggatct cggtcgcggg acggggtgtt gtgctcgaca tcaactatcg aaacacggcg
     1501 cagatcgtga acttcgcccg acgcctggtc gccggcgatg agtacgccga catcgaaggc
     1561 gtactcgccc ttggcgacgt cccgtccagt gtgccgcggg ccggagcgga gcccgtcatc
     1621 gcccgttgcg cgaaccggcg cgaactgaac gcgacgatga tctcccgcgt ccgcagcgtc
     1681 accaaggaca ttggcaccgg cataggtgac gtcgccgtac tctgcatcac gcgccgctct
     1741 gcgatggacg cgacagccgc actcctggcc gccggcttcc cggtcatcaa tctcgaggag
     1801 tacggcggcg tcccgctcga cgcagtcaag gtgggcacga tcaagcgcgc caaaggactg
     1861 gagttcaagc aggttctgat ccccgacatc cgccgtgaac acacgatcac cgagccacca
     1921 acagacgaga ccgaccgcga acgctgggac ctcacccggc gcgagctcta cgtcgccatg
     1981 accagggcac gagatggact ctgggtcagt atctcgaatt gagccgaccc cagatcgaag
     2041 atcggagaag tagcaaggtg ccagtattca cggacatccg aagcagatcg acccgtgacc
     2101 tgctggccgc atacgtcgag attctcgatg aactcttgcg acggaaggtc atccggactc
     2161 gaaacgctcc tgccggcgac ttggcggagc gggtggttgc cgaggcgtac tccggtgagc
     2221 tcgcctccaa ctcattgaag agctgggacg tccgggcttc ggatggtcgc ctcctccaag
     2281 tgaagagccg ggtgatcggc gccgacacca gccgatcgca ggtgttctcg ccgttccgaa
     2341 cctttgactt cgacacctgc gtcttcgtga tcttcgacgc gcgcgactac tcgatcagca
     2401 acgcaattga gcttccggca gagtctgtgc gcggcctctc ccatcgatcg gagtgggtag
     2461 ctgggaaccg agtcagagtg ttcgccgatc tgctgaacgc cccggctccg tcgacgtgac
     2521 cgctctgctg cgggacaccc tcgctgcgct ttgatcgaac cagatggctc gctctatcgg
     2581 cggacctgcg ttatcgagtg cgggaattgt tgcctcagcg tgcggtgatc gcgatcgtcg
     2641 gctgggtggg ttgtccggca cgctggcgat ctgtgaccgg tcaggtgctc gggtgtgtga
     2701 ggtcgatgcc gtgggcggct gcaagagctt gcagtttctc gatgtcgtcg atctggatgc
     2761 agagtgctgc gtgcaggccg gtcggttcgt ctcggatcag gtagtgggcg gtgctgtcga
     2821 taagtcgccg tgggtgtcga ggaagttcca gtgaacgtgc gctaggacga gtttctcggt
     2881 gagtggcttg gcttcgagga gttcataggt cactgagccg aggccgagct tctggtagag
     2941 ggggtaggac tgcttcagcc cggtcaccat ggctgctcgt gtttcgatca cgacggtgaa
     3001 gcggtcgtcg gcgatcacgc cgggcatgga ccatagttcg gcacccgctt cggcatcgag
     3061 ttcggtgagg gccgcagcgt agctggtcag gaactggtcg atctgtacct ggctgatgcc
     3121 catgccaacg agtatctccc gatgctccct agtggaccag ccagccaggg taggaactcc
     3181 tccctcaccg cgaggttcac gtgttggcgg gccagtcgct ccatggggtc gagttcagcg
     3241 gttggcgctg tagctgctca agtc
//
LOCUS       DAOPRQ010000144         3119 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_24900_length_3119_cov_13.489883, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000144 DAOPRQ010000000
VERSION     DAOPRQ010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 3119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3119
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24900_length_3119_cov_13.489883"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..863
                     /locus_tag="PKJ61_12175"
     CDS             <1..863
                     /locus_tag="PKJ61_12175"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOC14618.1"
                     /translation="DYLKACREASVAGRPGPNVVTMDGQRLDRYAATTINRRLAAISG
                     LFTFAGLRSAGAKNPVPKGREARWAAAGERSGMLAHTTRRRKPRSALRLREPKRLPVA
                     LSASEAGELVGSLRTWRDRAIAGLMLWSGLRSCEVLGLDVKDVDIGGRWVTVTGKGNK
                     QRRVPLDADVASVIDVYLLAERPASDCDRLFLVAKGPHRGQGLSPAGLRTIFRYHRQL
                     TGVAGGHPHALRHTFGTVLAEAGVDLAVMQALLGHAHVDTTARYVHLAPTHVKAEFDA
                     ARERIRNHHR"
     gene            826..2784
                     /locus_tag="PKJ61_12180"
     CDS             826..2784
                     /locus_tag="PKJ61_12180"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HOC14619.1"
                     /translation="MPHANASATTTVDGAGPGLRGEELLTAYLDRLGATGRGNVSYER
                     AARRFLRRWPDPQAWAALSLQDRLAEDTATRPLITFLMLHHGLAPGYDYLLERKLSSV
                     WREIHGSPLQGEIDRFLSASQQLGFSMRVRLATGSQVPVRLLIQTGRRIEDLTQDDLE
                     EFAAACHERTRRTGTSHNHYLSAISNAHRVLFHLGVLDSLPRSGGPTPLAERLAHVSP
                     PVRAELVAYLERKKATCQVKTVSAMATRLKHFGVFLAEVDPALSTVKDLDRRRHIEPW
                     LACLLEAVSERDGEPISIGDRNRRVVAVTTFLTDITEWGWDAAPPRKVLFRDDVPKLP
                     QLLPRYLPVDADRALTAALTQHPDNELAALALRLQRGCGLRIGELLDLELDCVHEIEG
                     NGAWLKVPLGKMATERMVPLDSETLALIDRITAIRSHGRPMPHPRYRRPAQFLFTYLG
                     RRLTQQGVRRELDHAAGVAGLAHVTTHQLRHTYATALVNAGVSLQALMALLGHVSAEM
                     SLRYGRLFDSTVRAEYERALDLAKAQVAQSDSAADMPGRTQLPLADITGGKDWRTTPL
                     LKSRMAGGFCLRAPAQGACSYANICEHCPSYRADPSSLPILAAQRVDAQALARDAQER
                     GWIDEAERHHKLIARLDALIANTGADTA"
     gene            2781..3071
                     /locus_tag="PKJ61_12185"
     CDS             2781..3071
                     /locus_tag="PKJ61_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009885260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6262 family protein"
                     /protein_id="HOC14620.1"
                     /translation="MTSSHTMNKVERACADLAHDGRPVTFAAIATATGLARSTLYRNA
                     ALRAIVEQHQRPADGPLTPLTDEIATLRAAVNSLADRVRSHDEQLRRLTRKN"
BASE COUNT          512 a         1166 c         1011 g          430 t
ORIGIN      
        1 tcgactatct gaaggcgtgc cgggaggcgt cggtggcagg gcggccgggc ccgaacgtgg
       61 tcaccatgga tggtcagcgt ctggaccggt acgcggccac gacgatcaac cgccgcctgg
      121 cggcgatctc gggactgttc accttcgccg ggctgcgctc cgccggggcc aagaacccgg
      181 tgcccaaagg gcgtgaggcg cggtgggcgg ccgctgggga gcggtccggg atgctggcgc
      241 acacgacacg gcgacgcaag cctcgttcgg cgttgcggct gcgtgagccc aagcggctgc
      301 cggtggcgct ctctgcttcc gaggccggcg agctggtggg aagcctgcgc acctggcgcg
      361 accgcgcgat cgccgggttg atgctgtggt ctggcctgcg ctcgtgcgag gtgctcggcc
      421 tggacgtcaa ggacgtcgac atcggcggta ggtgggtcac cgtcaccggc aagggaaaca
      481 agcagcgacg cgtcccgctg gacgccgacg tcgcctcggt gatcgacgtc tacctgctcg
      541 ccgagcggcc cgcctcggac tgtgaccggc tgttcctggt ggccaagggc ccgcaccgcg
      601 ggcaggggct gagcccggcc gggctgcgca cgatcttccg ctaccaccgg cagctgaccg
      661 gggtggcggg cggtcatccg cacgcgctac ggcacacctt cggcaccgtc ctggccgagg
      721 ccggcgtcga cctcgcggtg atgcaggcgc tgctcggaca cgcccacgtg gataccaccg
      781 cacggtatgt ccatctcgcc ccgacccacg tgaaagccga gttcgatgcc gcacgcgaac
      841 gcatccgcaa ccaccaccgt tgacggagct ggtccgggct tgcgcggtga ggaactgctg
      901 accgcttacc tggaccggct gggtgccacc ggtcgcggca acgtctccta cgagcgggcc
      961 gcccgaaggt tcctgcgccg ctggcccgac ccccaagcct gggccgcgct ttccttgcag
     1021 gacaggcttg ccgaggacac cgccactcgt ccactgatca cgttcctgat gctgcaccat
     1081 gggctggcgc cgggctatga ctacctgctg gaacgcaagc tgtcctcggt atggcgggag
     1141 atccacggca gcccgctgca gggcgagatc gaccgcttcc tgtccgcctc gcagcagctg
     1201 gggttctcga tgcgggtccg gctggcgacc ggctcgcagg tgcccgtgcg gttgttgatt
     1261 caaaccggta ggcgaatcga ggacctcacc caggacgacc tggaagagtt cgccgcggcc
     1321 tgccacgagc ggacccgtag gaccgggacc agccacaacc actacctgtc ggcgatcagc
     1381 aacgcccacc gggtcttgtt ccatctcggc gtcctggact cgttgccccg atcgggtggg
     1441 ccgaccccgc tggcggagcg gctcgcccac gtgtcaccac cggtccgggc ggagctggtc
     1501 gcctacctgg aacgcaagaa ggcgacctgc caggtcaaga ccgtctcggc gatggccacc
     1561 cgcctgaagc acttcggcgt cttcctggcc gaggtggacc ccgccctgag cacggtcaag
     1621 gacctggacc ggcgccgaca catcgaaccc tggctcgcct gcctcctcga ggcggtcagc
     1681 gagagggacg gtgagccgat cagcatcggc gaccgcaacc ggcgggtggt ggccgtgacc
     1741 acgttcctga ccgacatcac cgagtggggc tgggacgccg cacccccgcg caaggtgctc
     1801 ttccgcgatg acgtccccaa gctgccgcag ctgctgccgc gctacctacc agtggacgcc
     1861 gaccgggccc tgaccgccgc actgacccag caccccgaca acgaactcgc cgcgctggcg
     1921 cttcggctgc aacgcggctg cgggctacgg atcggtgagc tgctcgacct cgagctggac
     1981 tgcgtacacg agatcgaggg caacggcgcc tggctgaagg tcccgctcgg caagatggcc
     2041 accgaacgga tggtcccgct cgattccgag accctggcgc tgatcgaccg gatcaccgcg
     2101 atccgttccc acggccgccc gatgccgcac ccgcgctacc ggcgcccagc ccagttcctg
     2161 ttcacctacc tcgggcgccg gctgacccag caaggcgtac gccgagagct cgatcacgcg
     2221 gccggcgtcg ccggactcgc ccacgtcacc acccaccagc tgcgccacac ctacgccacc
     2281 gccctggtca acgccggagt ctccctgcag gccctgatgg cgctgctcgg gcacgtcagc
     2341 gccgagatga gcctgcgcta cgggcggctg ttcgactcca ccgtccgggc cgagtacgaa
     2401 cgagccctgg acctggccaa agcccaagtc gcgcaaagcg actcggccgc ggacatgccg
     2461 ggcaggaccc aattgccgct ggccgacatc accggcggta aggactggcg caccacaccc
     2521 ctgttgaagt cccgcatggc cggcggcttc tgcttgcgcg caccagccca gggcgcctgc
     2581 agctacgcca acatctgcga gcactgtccc agctaccgag ccgacccctc ctccctgccg
     2641 atcctggccg ctcaacgcgt cgacgcccaa gccctggccc gcgacgcaca agaacgagga
     2701 tggatcgacg aagccgaacg ccaccacaaa ctcatcgcgc gcctcgacgc cctgatcgcc
     2761 aacacgggag cggacaccgc atgaccagca gccacaccat gaacaaggtc gagcgcgcct
     2821 gcgccgacct ggcccatgac gggcgaccgg tcacgttcgc cgcgatcgcg accgctaccg
     2881 gactcgcccg atccaccctg taccgcaacg ccgcgctacg ggccatcgtc gagcagcacc
     2941 agcgccccgc cgatggaccg ctaacgccgc tgaccgacga gatcgcgaca ctgcgcgccg
     3001 ccgtgaacag cctcgccgac cgggtccgca gccacgacga acaactccgc cgcctcaccc
     3061 gcaagaactg aaggcgttaa ccggccaacg cccctcccgg accccgatta gccggataa
//
LOCUS       DAOPRQ010000145         3107 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_25024_length_3107_cov_9.176933, whole genome shotgun sequence.
ACCESSION   DAOPRQ010000145 DAOPRQ010000000
VERSION     DAOPRQ010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 3107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3107
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25024_length_3107_cov_9.176933"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            315..1670
                     /locus_tag="PKJ61_12190"
     CDS             315..1670
                     /locus_tag="PKJ61_12190"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HOC14621.1"
                     /translation="MRYIGMDVHREFAQLAVVEDGLLRDEGRIGVTPEALREWAAGLG
                     PDDQVALEATGNSEAIAMLLTPLVARVVVSNPLKTRAIAEAKVKTDKVDARILAQLLA
                     ADFLPGVWLPDERTQMLRRQVVRRAGIVRHRTRVKNQVHAILARNLCPTPPVSDLFGK
                     AGRYWLGQQALPPDETAAVAALLRQLDFAGQELHRVDRELAVEALADPVVARLMTIPG
                     IDAIAGISIVAAVGDFTRFDDPDKLVCYLGLNPRVRQSGNSAPVHGRISKTGRAQVRG
                     VLVEAAWSACRAPGPLRAFYRRVQARRGFPKAIVATARKLTILAWHLAVHNEDYAFAR
                     PGLVAHKRRKLELAAGQPSRRGFHGTPRAAYNDKDRRDAETAIAEQAEHAYEVLVAGW
                     QAHRPANRQGSGADHLDAVLAGVKAEPAGALRAALTPAPGGFLRQPTSPADWSSPHYT
                     P"
     gene            complement(1672..2718)
                     /locus_tag="PKJ61_12195"
                     /pseudo
     CDS             complement(1672..2718)
                     /locus_tag="PKJ61_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015068842.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT          509 a         1027 c         1015 g          556 t
ORIGIN      
        1 cccgggcctt cacggctttg cggatgcgag cgttgaccga ctcgatcgcg ttcgtaaccg
       61 gtatgaaagg tcgcgagggc ccgcgtgttg tcccgctggc tgggcgtctt cgctcgagcg
      121 tacggtgaga acgtccggga caggggctct tggggaaacc gtgcgggcag gccgttcttg
      181 cacgatgagc tgtgagctcg ggttagtaat tggccgcccc tgcgactggt ccggctgcgc
      241 tgcgagcgcg tatccgtagg tgtcgtttcg tccccacggt tccccttcgt tgaaggagaa
      301 gggagctgga gaagatgcgg tacatcggga tggatgtgca tcgtgagttc gcccagttgg
      361 ctgtggtcga ggacgggctg cttcgcgatg agggccggat tggggtgacg ccggaggcgt
      421 tgcgggagtg ggcggccggg ctcggcccgg atgatcaggt ggcgttggag gcgactggga
      481 acagtgaagc gatcgcgatg cttctcaccc cgctggttgc ccgggtggtg gtgtcgaacc
      541 cgttgaagac gagggcgata gccgaggcaa aggtgaagac cgacaaggtc gatgccagga
      601 tcctcgcgca gttgttggcg gccgacttcc tgccgggagt gtggcttccg gatgagcgaa
      661 cccagatgct gcgtcgccag gtcgtgcgcc gcgccgggat cgtccggcac cggacccggg
      721 tgaagaacca ggtgcacgcg atcctggcca ggaacttgtg cccaacaccg ccggtgagtg
      781 atctgttcgg caaggccggc aggtactggc tgggccaaca ggcgctgccc cccgatgaga
      841 ccgctgcggt cgccgcgttg ctgcggcagc tcgacttcgc cggacaggaa cttcatcggg
      901 tcgatcgcga actcgcggtg gaggctttgg ccgacccggt cgtggcacga ctgatgacga
      961 tccccgggat tgatgcgatc gcgggtatct cgatcgtggc tgcggtcggc gacttcaccc
     1021 ggttcgacga tccggacaag ctggtgtgct acctcgggct gaaccctcgg gtgcgtcagt
     1081 ccgggaacag cgccccggtc cacggccgga tctccaagac cggacgagcg caggtccgag
     1141 gtgttctggt cgaggcggcc tggtcggcct gcagagcccc cgggccgctg cgagccttct
     1201 atcggcgggt ccaggcccgg cgcggtttcc ccaaggcgat cgtggcgacc gcccgcaagc
     1261 tcacgatcct cgcctggcac ctcgctgtcc acaacgagga ctacgccttc gctaggcccg
     1321 ggctggtggc gcacaagcgg cgcaagcttg aactcgccgc agggcaaccg tctcgacgcg
     1381 ggttccacgg cacaccgagg gccgcctata acgacaaaga ccgtcgcgac gcggaaaccg
     1441 cgatagctga gcaagccgag cacgcctacg aggttctggt cgctggctgg caagcccacc
     1501 gaccggccaa ccgtcagggc tcgggggctg atcatcttga tgccgtcctc gccggggtca
     1561 aggccgagcc tgccggtgcc ctccgggcag ccttgacccc ggctccgggc ggcttcctca
     1621 ggcagccaac gagccctgcc gattggagca gccctcacta caccccttga cgatttcatc
     1681 cgtggtgcac acgattcggc ggatctcccg gtcgaactgg aggaacggca cgaactcggc
     1741 ccaggcgttg gaccacagcc ggatcacggc cgggtacttg gcgccccatg tctcctggaa
     1801 ctccaagaag cgttcctcgg cctgttcgag ggtagcggcc tggtacaccg gtttcaggtc
     1861 gcgggtgatc gcttcccagt cctgccgggc cgcgtaccgg taggtgttcc tcatcagatg
     1921 cacgatgcac gtctgaacga tcgtgtccgg ccacaccgcg ttgacagctt cgggtaggtg
     1981 tttcagcccg tcgcacacca gcatcagaac gtcttcgacg ccgcggttct tgatctcggt
     2041 caggacctgc tgccagtact tcgcgccttc gccgccatcc ccggcccaca agcccaggat
     2101 gtcccggttc ccgtccacgg tgaccgccaa cgcgatgtag atcggccggt tcgcgacctg
     2161 cccgtcgcgg atcttcacat gaatgcagtc gatgaacacc accggataga ccgggtcgag
     2221 cggccggccc tgccattcgg ccatcccgtc cagcaccttg tcggtgatcg tcgagatcgt
     2281 ggtcttcgac acctccgagc cgtacacgtc ggccaggtgc gcagcgatgt cgccatgggt
     2341 cattcccctg gccgacaacg acaacacgat gtcctcgatc gagcccagcc ggcgctgcct
     2401 cttcgcaacc accaccggcg tgaacgtgcc cgcccgatcc cgaggaacct cgatctcgat
     2461 cggccccgcc ttcgtcaaca cggtcttcgg ccgagtgccg ttgcgatagt tcccgccgtt
     2521 ctcggtccgg acatgcttgt cgtagcccaa atgggcactc agctcgccct cgagcgccga
     2581 ctcgacgacc agcttggtca gctgcgccag cagaccgccc tcgccatcga ccgacaaccc
     2641 ctgccgctgg gcatcaccca ccagccgggc caccagctgc ttgtccacca ccaacggcgg
     2701 ctgctcgacc gtcgccacct gctcctccaa ctcggccgcc tcgacgacga ccggctcaac
     2761 ctgcgtgctc atgatgtacc tccctgatcg gagttacacc gttgaaagta cagtcccggc
     2821 tcaagaccga gctgaagatt ctgcacggga tcaggctcgg aactcacccc atcgccttcg
     2881 gtttcgcccc ttgcactctc cactgggtca gacacggctg tggaggtgct ggttgagagg
     2941 tgggtcgggc gggtgctcgc acttgggtca gacacgactg tggaggtgct ggttgagacg
     3001 taggtcgggc gggtactcgc attcgacgat cggtcggatt cgaccgacgc atccgaggtg
     3061 aagggccacg atcccgagat ggccgcccaa atcacgctga cagcgag
//
LOCUS       DAOPRQ010000146         3048 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_25702_length_3048_cov_9.504511, whole genome shotgun sequence.
ACCESSION   DAOPRQ010000146 DAOPRQ010000000
VERSION     DAOPRQ010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3048
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25702_length_3048_cov_9.504511"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2509
                     /locus_tag="PKJ61_12200"
     CDS             <1..2509
                     /locus_tag="PKJ61_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit A"
                     /protein_id="HOC14622.1"
                     /translation="TTVFSYLLIGHDPTRRANRSAALTALIVTTTGGLAMLVGFLALG
                     QAAGTFSLAAILGAQPSGPLVTLAALLILVGALSKSALVPFHFWLPGAMAAPTPISAY
                     LHAAAMVKAGVYLVAVLAPVLAGSPGWRPAVWTLGAMTLFLGGWRALRQDDLKLLLAY
                     GTVSQLGLLVLLLGTGTEAAALGGLAMVLAHALFKATLFLVVGVVDHSTGTRQLSQLS
                     GLGRKLPVTATVAAFAAASMAGIPPTLGFIAKEAGLEGITNLALTGDGTGIAPVPAWA
                     LTTVIMVGSAITVAYSLRFWWGAFGNKRGVADAELSHAPERGFVAVPVLLGLASLVGG
                     FLGGPLTQALAPYAESLGLGEPSHGLKLIPAFGLPLLVSTVAIVGGAILFWQRKRIAA
                     VQQTFPEVPGAVDFYRLTMAQLDRLAVEVTARTQRGSLAHYVGTILVVLTAAIFFTLS
                     GIREWPAVRLADNWGQVAVGSLMVAAALLVAFSRGRLRAVLLLGVTGYGTAVLFLLHG
                     APDLALTQVLAETVSLLVFLLVLRKLPKFFTERPLTSRRWWRVVIAAGTGLAFTLVAL
                     LMLGSRTATPVSAPYSKTAYEFGYGNNIVNVVLVDTRAWDTIGEIAVLSIAATGVASL
                     IFLRGRVTPLHRPSLPPEGPTRGWLQGSRALPAEQRSMVFEVVTRLLFPVMILVSLYL
                     LIAGHNLPGGGFAGGLVAGTALLIRYLAGGGQELDEALPVPAGVLLGTGLVLSVLTAV
                     FPLTFGGLIFQSYDVHLRIPGWESLATPFGEWTLFGDIHLVTSTVFDIGVYLVVIGMM
                     LDLGRSLGTGIDSHARDELTPAPLPDSTHAIPGRKWA"
     gene            2500..2937
                     /locus_tag="PKJ61_12205"
     CDS             2500..2937
                     /locus_tag="PKJ61_12205"
                     /inference="COORDINATES: protein motif:HMM:NF005929.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter subunit C"
                     /protein_id="HOC14623.1"
                     /translation="MGLMPANLTLAVVAGILIACGVYLITERSLSRILVGVVLASNGV
                     SVLFLVASGPAGGAPIIGLTPPEKMSDPLPQAMVLTAIVITLSVAAFVMTMAYRSFQI
                     NGQDEVQDDVEDARIAALAKADQTSETYEYLGFSDTGEDVVSE"
BASE COUNT          385 a         1118 c         1000 g          545 t
ORIGIN      
        1 caccacggtc ttctcctacc tcctgatcgg ccacgacccg acccgccgcg ccaaccgctc
       61 ggccgcgctc accgcgttga tcgtcaccac gaccggcggc ctcgcgatgc tggtcgggtt
      121 cctcgcgctc ggtcaagctg ccggcacctt cagcctcgcc gccatcctgg gcgcgcagcc
      181 gtccgggccg ttggtcacgc tggctgcgct gttgatactc gtcggcgcgc tgagcaagtc
      241 ggcgctcgtt ccgttccact tctggctgcc gggcgcgatg gccgcgccca ccccgatctc
      301 ggcctacctg cacgccgcag ccatggtgaa ggccggcgtc tacctggtgg ccgtcctggc
      361 cccggtcctc gcggggtcgc cgggctggcg tccggcggtc tggacgctcg gcgcgatgac
      421 cctcttcctg ggtggctggc gagcactgcg ccaggacgac ctcaaactgc tgctcgccta
      481 cggcacggtc agccagctcg gtctgctggt gctgctactg ggcaccggca ccgaagcggc
      541 agccctgggt ggcctggcca tggtcttggc ccacgctttg ttcaaggcga ccctcttcct
      601 cgtcgtcggc gtggtcgacc actccactgg cacccgccag ctcagccagc tcagcggact
      661 cggccgcaag ctaccggtca ccgcgaccgt cgcggcgttc gccgctgcgt ccatggccgg
      721 catcccgcca acgctcggct tcatcgccaa ggaagcaggg cttgagggca tcaccaacct
      781 cgcgctgacc ggtgacggca ccggcatcgc cccggtgccc gcctgggccc tgaccacagt
      841 gatcatggtc ggctcggcga tcacggtcgc gtactcgctg cgcttctggt ggggtgcgtt
      901 cggcaataag cgcggcgtag cggacgccga actcagccac gcccccgagc gcggcttcgt
      961 cgccgtcccg gtgctcctgg gactcgccag cctggtcggc ggcttccttg gtggaccgct
     1021 cacccaggcg ctcgcaccgt acgccgagtc gctcgggctt ggcgaaccca gccacggcct
     1081 caagctgatc ccggcgttcg ggctgccgct gctggtgagc acggtcgcga tcgtcggcgg
     1141 cgcgatcctg ttctggcagc gcaagcgcat cgctgccgtc cagcagacct tccccgaagt
     1201 gcccggcgcc gtcgacttct accgcctgac catggcgcag ctcgaccggc tcgcggtcga
     1261 agtcaccgcc cgcacccagc gcggctcgtt ggcgcactac gtcggcacga tcctggtggt
     1321 gctcaccgcc gccatcttct tcacgctctc cggcatccgc gaatggccag ccgtccggct
     1381 cgccgacaac tgggggcagg tcgcggtggg ctccctgatg gtggctgcgg cgctcctggt
     1441 cgccttctcc cgcggacgcc tgcgcgccgt cctgctgctc ggggtgaccg gctacgggac
     1501 ggccgtgctg ttcctgctgc acggcgcacc cgacctggca ctcacccagg tgctggcgga
     1561 gaccgtgtcg ctgctcgtct tcctgctggt gctgcgcaag ctgccgaagt tcttcaccga
     1621 gcgtccgctc acctcgagac gctggtggcg ggtcgtgatc gccgccggca ccggtctggc
     1681 gttcaccctg gtcgccctgc tgatgctggg ttcccggacg gcaaccccgg tttcggcgcc
     1741 ctactcgaag accgcctacg agttcggcta cggcaacaac atcgtgaacg tcgtgctggt
     1801 ggacacccgg gcctgggaca ccatcggcga gatcgcggtg ctgagcatcg ccgcgaccgg
     1861 tgtggccagc ctgatcttcc tgcgcggacg ggtgacgccg ctgcaccgtc cgtcgctgcc
     1921 ccctgaaggg ccgacccggg gctggctgca gggcagccga gccctacccg ccgagcagcg
     1981 ttcgatggtg ttcgaggtgg tcacacggct gcttttcccg gtgatgatcc tggtctcgct
     2041 ctacttgctg atcgccgggc acaaccttcc cggcggcggg ttcgccggcg gcctggtggc
     2101 gggcacggcg ctgctgatcc gctacctcgc cggcggcgga caggaactgg acgaagccct
     2161 gccggtgccg gccggcgtcc tgctcggcac cgggctggtg ctgtcggtgc tgaccgcggt
     2221 cttcccgctc accttcgggg gcctgatctt ccagagctac gacgtgcatc tgcgcattcc
     2281 aggctgggag tcgctggcta cgccctttgg cgagtggacg ctgttcgggg acatccacct
     2341 ggtcacgtcg accgtgttcg acatcggggt ctacctggtc gtgatcggca tgatgctcga
     2401 cctgggccgc agtctcggca ccggcatcga ttcgcacgcc cgcgatgaac tcacccccgc
     2461 tcccctgccc gactccacgc atgcgatccc cggaaggaaa tgggcctgat gccggcgaac
     2521 ctcaccctgg cggtggttgc aggcatcctg atcgcctgcg gggtctacct gatcaccgag
     2581 cggtcgctgt cgcgcatcct ggtgggcgtg gtgctggcca gcaacggggt cagcgtcctg
     2641 ttcctggtgg cgtccgggcc ggccggcgga gctccgatca tcggactcac cccgcccgag
     2701 aagatgagcg acccgctgcc ccaagcgatg gtgctgaccg cgatcgtgat cactttgagc
     2761 gtggccgcgt tcgtgatgac catggcctac cgcagcttcc agatcaacgg ccaggacgag
     2821 gttcaggacg acgtcgagga cgcccggatc gcggccttgg ccaaggccga ccagacgtcg
     2881 gagacctacg agtacctggg cttctccgac accggtgagg atgtggtgag cgaatgaaca
     2941 atctgcttgc cgtgcccacg ctgctctgcc tcctcggcgc ggcgatcacc ttcctggccg
     3001 gccgccatcc tgccgtccag cgcgcgatct cagtcaccgc ggtggccg
//
LOCUS       DAOPRQ010000147         2938 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_27003_length_2938_cov_11.544572, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000147 DAOPRQ010000000
VERSION     DAOPRQ010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27003_length_2938_cov_11.544572"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..729)
                     /locus_tag="PKJ61_12210"
     CDS             complement(<1..729)
                     /locus_tag="PKJ61_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HOC14624.1"
                     /translation="MARAVGIDLGTTNSVVAVLEGGEPTVIPNAEGARTTPSVVAFAK
                     GGEVLVGEVAKRQAVTNVDRTIRSVKRHMGTNWSVSIDDKKYTPQEISARVLMKLKRD
                     AEAYLGEPVTNAVITVPAYFGDAERQATKEAGEIAGLVVDRIINEPTAAALAYGLDKG
                     HLEQTVLVFDLGGGTFDVSLLEIAEGVFEVKATKGDARLGGDDWDQRVVDWLVTQFKN
                     KFGIDLGKDKMARQRLQEAAERAKI"
     gene            complement(864..1784)
                     /locus_tag="PKJ61_12215"
     CDS             complement(864..1784)
                     /locus_tag="PKJ61_12215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14625.1"
                     /translation="MDVVQRDRISLAVDPRVVPERPQVPVRQFTLRPDGLAPLDALIA
                     TPADSHGVLVYFPGFNTPLGPWEMAKCAHLADVTGLTVVLTEIPGMSRFGAPIPKSIR
                     KDMLAGRIDSWTQLNLTYIAVALRDVGNLEFLQVLGFSTGCSLAVSALPVLATWGPIE
                     GLNLVEPVAITQRNLASLQAHNLADLGRMPAALATNTGHDWVMHARKGQRREPPVRYS
                     PVDMIAIAQVLVTEKLQSQLLDVDLARCALVRGARSSLCREPAFDKLDRRLAEQGVPG
                     PTITVTGLGHQLWHSFPMVVQLTKAMLGPL"
     gene            1941..2882
                     /locus_tag="PKJ61_12220"
     CDS             1941..2882
                     /locus_tag="PKJ61_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HOC14626.1"
                     /translation="MSWVDPWLRGSLLALSRSESFKRLAVRLPMTASVVARFVPGETV
                     ADCLAAVRSLASEGLTATIDYLGEDTTTPEQAAAIRDGYLELITELSAAGLCEMAEVS
                     VKLTALGVGLPEGRELALGHAREICAAATDAGTTVTLDAEDHTTTDTTIGILAELRRE
                     FPETGAVLQAYLHRTPADCELLSGAGSRVRLCKGAYAEPDEVAHQTREEVSAAYVACS
                     RILLAGDGYPMLATHDPEMIAAVDELARQLGRAPGTYELQMLYGIRSDEQRRLAASGE
                     RVRVYVPFGTDWWGYFIRRLAERPANLAFFARALFGR"
BASE COUNT          457 a          968 c         1017 g          496 t
ORIGIN      
        1 gatcttggcg cgctcggcgg cctcctggag gcgctggcgg gccatcttgt ccttgcccag
       61 gtcgatgccg aacttgttct tgaactgggt gaccagccag tcgacgacgc gctggtccca
      121 gtcgtcaccg ccgaggcgag cgtcgccctt ggtggccttc acctcgaaga cgccctcggc
      181 gatctccagc agggacacgt cgaaggtgcc gccgcccagg tcgaagacga gcacggtctg
      241 ctcgaggtgg cccttgtcga ggccgtaggc cagcgcagcg gcggtgggct cgttgatgat
      301 ccggtcgacc accaggcccg cgatctcgcc ggcttccttg gtggcctggc gctccgcgtc
      361 cccgaagtac gccgggacgg tgatcaccgc gttggtgacc ggctcaccga ggtacgcctc
      421 ggcgtcccgc ttgagcttca tcagcacccg ggcgctgatc tcctgcgggg tgtacttctt
      481 gtcgtcgatg gaaaccgacc agttggtgcc catgtgccgc ttcaccgaac ggatcgtccg
      541 atcgacgttg gtgacggcct ggcgcttggc gacctcaccc accaggacct ctccgccctt
      601 cgcgaaggca accacggacg gcgtggtgcg cgcgccctcg gcgttgggga tgacggtggg
      661 ctcgccgccc tcgaggacgg cgacgacaga gttggtggtg ccgaggtcga tgccgactgc
      721 tcgtgccatg tgagttacct ccggttgagt ctgaacgaga ttgagcatag caggctcaat
      781 ctcaaggttg agtacacccg actcaactac gttgcatggt cagctattcc cgctgggcgg
      841 ctctggtctc aaaggctcga cggtcagagc gggccgagca tggccttggt gagctgcacc
      901 accatcggga acgagtgcca cagttggtga ccgagccccg tcaccgtgat cgtcggaccg
      961 ggcacgccct gttcggcgag cctgcggtcg agcttgtcga aggccggttc gcggcaaagt
     1021 gagctgcgtg cgccgcgaac cagggcgcat cgggcgagat cgacgtccag caattggctt
     1081 tgcagcttct cggtgaccag cacttgggcg atcgcgatca tgtcgactgg cgagtagcgg
     1141 acgggcggct cgcgccgctg gcccttgcgc gcgtgcatca cccagtcgtg cccggtgttg
     1201 gtggccaacg ctgccggcat ccgaccgagg tcggcaaggt tgtgcgcctg cagggacgcg
     1261 aggttgcgct gggtgatcgc gaccggctcc acgaggttca ggccctcgat cggcccccag
     1321 gtggccagca ctggcagcgc gctcactgcg agcgagcagc cggtggaaaa ccccagtacc
     1381 tgcaggaact cgagattgcc gacatcgcgc agcgcaacag ctatataggt gagattgagt
     1441 tgcgtccagg agtcaatgcg cccggccagc atgtccttgc ggatcgactt cgggatcggc
     1501 gccccgaatc ggctcatgcc agggatctcg gtgagcacca cggtcaggcc ggtgacgtcc
     1561 gccagatggg cgcacttggc catttcccag gggccgagcg gggtgttgaa accagggaag
     1621 tagaccagca caccgtggct gtcagcgggg gtcgcgatca gcgcatccag cggcgccaaa
     1681 ccgtccggtc ggagcgtgaa ctgccgcacc ggtacctgcg gacgctccgg caccacgcgg
     1741 gggtcgacgg cgaggctgat ccggtcccgc tgcaccacgt ccacgcttgg atggtagtac
     1801 gccggcaggt ggcgagtggg tcggtcagga tgcgcgagaa tgaggcccat gaacgacctc
     1861 gaaggcacag ttgcggatct tcgtggagga ttgtgtaccg agttcggcac acaatcctcc
     1921 acgctgatcg gcggtccgag atgagctggg tggacccctg gctgcgtggc tccctgctcg
     1981 cgctgtcgcg gagcgagtcg ttcaagcggc tggcggtacg gctgccgatg accgcgtccg
     2041 tggtggcccg gttcgtgcct ggcgagaccg ttgccgactg cctggccgcg gtgcgctcgc
     2101 tcgcctccga agggctgacg gccaccatcg actacctcgg cgaggacacc acgacgcccg
     2161 agcaggccgc tgccatccgc gacggctacc tcgaactgat caccgagctg agcgcggccg
     2221 gactctgcga gatggccgag gtctcggtga agctgaccgc ccttggcgtt ggcctgccgg
     2281 aagggcgtga gctcgcgctc gggcacgctc gcgagatctg cgccgcggca accgacgccg
     2341 gcacgaccgt caccctggac gccgaggatc acaccaccac cgacaccaca atcggcatcc
     2401 tggccgagtt gcgccgcgag ttcccggaaa ccggagcggt gctgcaggcc tacctgcacc
     2461 ggacgccggc cgactgcgaa ctgctgtcgg gcgccggttc gcgcgtccgg ttgtgcaagg
     2521 gcgcgtatgc cgagccggac gaggtggcgc accagacccg cgaagaggtg tccgcggctt
     2581 acgtcgcgtg ctcgcggatc ctgctcgccg gggacgggta cccgatgctc gccacccatg
     2641 acccggagat gatcgccgcc gtggacgaac tggctcggca gttgggccgc gcgcccggca
     2701 cctacgaact gcagatgctc tacgggatcc gatcggacga acagcgccgg ctggctgcat
     2761 ccggcgagcg ggtgcgggtg tacgtcccct tcggcaccga ctggtggggc tacttcatcc
     2821 gccggttggc cgagcgtccg gcgaacctgg cgttcttcgc gcgggcattg ttcggccgct
     2881 gagcacgcgc tccctacgac atccgggcgt caactcagcc caggccggga tctcgttt
//
LOCUS       DAOPRQ010000148         2876 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_27784_length_2876_cov_10.475009, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000148 DAOPRQ010000000
VERSION     DAOPRQ010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27784_length_2876_cov_10.475009"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(250..747)
                     /locus_tag="PKJ61_12225"
     CDS             complement(250..747)
                     /locus_tag="PKJ61_12225"
                     /inference="COORDINATES: protein motif:HMM:NF013183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HOC14627.1"
                     /translation="MADQLLATVITVSDEVFSGADADRGGPVAAELLSELGVAVERKV
                     VPDDGDRIEAAIRAAMTAGARFVMACGGTGIGPKDRTPEVVARLLTFQMPGIAEEIRR
                     LGIAHAKPALVSREVAGAIVPEVGAPVFVLAAPGSRGGVRDALSVVGPILDYVIEQLD
                     GAGHR"
     gene            complement(757..1914)
                     /locus_tag="PKJ61_12230"
     CDS             complement(757..1914)
                     /locus_tag="PKJ61_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HOC14628.1"
                     /translation="MHVTDPRATYDAWRAEPLARDASGRPVLCRHADVVAAATDPATY
                     SSTVSRFLQVPNGLDGAEHRAARDLLDPFFGPDRMAELEPVIARVAAEVVAALPSEVD
                     AVLDLGARFAVRAQSAWLGWPAELEPELLGWMADNHSANRSGELVRTREVAERFDAII
                     RSLLDARRESPQADVTTELVRLQDAAGEPRAPHPSSRRMPGSADDSFTLSEEALVSVL
                     RNWTGGDLGSLALCTGVVAHWLVSHPEHQDELLAADDAALDAAIDEILRIDDPFVSNR
                     RLTTRDTAIGGQPIAAGERVVLNWTAANRDPAVFGDPDHYDPVGNAPHNLVYGIGPHV
                     CPGRPLATLELRLLVRALLSAARLEPSEQRAERELPPLGGFSEVPVRLVRR"
     gene            complement(1984..>2876)
                     /locus_tag="PKJ61_12235"
     CDS             complement(1984..>2876)
                     /locus_tag="PKJ61_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOC14629.1"
                     /translation="GNFGVSLVQLLTPWLIGLGMLWWMGQSQMMKIAGKPDREVWYQA
                     ASFVWLPFILIGAWIAWQYLKSVPIKANISQQFDIFANQDTWWMTLLYIMTFGTFSGL
                     SAQFGLLMGNLYGAANPGIVQGTGATAQILVAGYQVPDPTAFVFLGPLVGAGARVLFS
                     PLTDRMGGAIWTLISGIGLVASILWTIPALTPDVSSAATLQGGFNQFLWGMLAIFLFA
                     GIGNASTFKQMPMIFERRQAGGVIGWTGAIAAFGPFIFGVGLTMMPAPVFYWIGVVWA
                     VMCIAITWWRYARPGAPKKS"
BASE COUNT          482 a          999 c          975 g          420 t
ORIGIN      
        1 ccaagacccc cgcgccggag gccaaggcga cggcacacca gcctccggtg cgggatccag
       61 tcgaagcgtt gtcctcggac aactttggcg gtggcggcgg cctttcgtga cgccgtcgct
      121 cgttcctcac gacggctcct cagggaccgt ttgggatggc gcaacctcgc tcccgaaagg
      181 gactctcgct cccggattat cgggagcaaa ggtccctatc gggagcgaag ttcgcttggg
      241 gcggccgtgt cagcggtggc cggcgccgtc gagttgctcg atcacgtagt ccaggatcgg
      301 gccgaccacg ctcagggcgt cgcggacgcc accgcgcgag ccgggagcgg ccagcacgaa
      361 caccggcgca cccacctcgg ggacgatcgc cccggccacc tcgcgcgaga ccagggctgg
      421 cttggcgtgc gcgatgccga gccggcggat ctcttcggcg atcccgggca tctggaaggt
      481 gagcagccgg gccaccacct cgggggtgcg gtccttcggg ccgatgccgg tgccgccgca
      541 ggccatcacg aagcgcgcac cggcggtcat cgccgcccga atcgcggcct cgatccggtc
      601 cccgtcgtcg ggcaccacct tgcgttcgac agccacgccg agttcgctca gcagttcggc
      661 ggccaccggg ccgccgcggt ccgcgtccgc gccggagaag acctcgtcgg aaaccgtgat
      721 caccgtagcc agcagttggt cagccatgct tgcaggctag cgccggacga gccgcaccgg
      781 cacctcgctg aacccaccca gcggcggcaa ctcgcgctcg gcccgctgtt cggacggctc
      841 cagccgcgcg gcgctcaaca aggcccgcac cagcagccgc aactccaggg tggccagcgg
      901 ccgtcccggg cagacgtgcg gcccgatgcc gtagaccagg ttgtggggcg cattgccgac
      961 cggatcgtag tgatcgggat cgccgaacac cgccgggtca cggttcgcgg ccgtccagtt
     1021 cagcaccacc cgctcgccgg ccgcgatcgg ctggccgccg atcgcggtat cccgggtagt
     1081 cagccgccgg ttcgagacga aggggtcgtc gatccgcaga atctcgtcga tcgccgcgtc
     1141 cagcgcggca tcgtcggcgg ccagcagttc gtcctggtgc tcgggatgcg aaaccagcca
     1201 gtgcgccacc actccggtgc acagcgccag tgagccgagg tcgccgccgg tccagttccg
     1261 cagtaccgaa accagcgctt cctcgctgag ggtgaagcta tcgtcggcag atcccggcat
     1321 tcgccgggat gacggatggg gagcgcgcgg ctcgcccgca gcgtcctgca gccgcaccag
     1381 ctcggtagtc acatcggctt gcggcgactc cctgcgggcg tccaggagcg agcggatgat
     1441 cgcgtcgaag cgctcggcca cctcgcgggt gcgcacgagt tcgccggagc ggttggcgga
     1501 atggttgtcg gccatccagc ccagcaactc cggctccagt tcggccggcc agcccagcca
     1561 cgcagattgg gcgcggacgg cgaaccgggc gcccaggtcc agcaccgcgt ccacctcgga
     1621 cggcaacgcc gccaccactt cggctgccac ccgcgcgatc acaggctcca actcggccat
     1681 ccgatccggt ccgaagaacg ggtcgagcag gtcgcgggcc gcccggtgct cggccccgtc
     1741 cagcccgttg ggcacctgca ggaaacggga gaccgtgctg gagtaggtgg ccggatcggt
     1801 tgccgcggcc accacatccg catgccggca cagcaccggt cgtccgctcg cgtccctggc
     1861 gagcggttcg gctcgccagg cgtcgtaggt ggcgcgtggg tcggtgacgt gcatctttct
     1921 ccctgcgtga gtgccgaacg tgctcgatgg tctcgacgag ctcgaccagc gaaccgtcga
     1981 gactcaggac ttcttcggtg ctcctggacg ggcgtaacgc caccacgtga tcgcgatgca
     2041 catgaccgcc cagacgacgc cgatccagta gaagaccggt gccggcatca tggtgagtcc
     2101 taccccgaag atgaacggtc cgaacgctgc gatcgcacca gtccagccga tcacgccgcc
     2161 agcctggcga cgttcgaaga tcatcggcat ctgcttgaag gtggacgcgt tgccgattcc
     2221 ggcgaacagg aagatcgcca gcatgcccca taggaactgg ttgaacccgc cttgcagggt
     2281 cgccgcgctg ctcacatcag gggtgagtgc tgggatggtc cacaggatgc tggcgaccag
     2341 gccgatgccg gagatcagcg tccagatcgc tccgcccatg cggtcggtga gtggcgagaa
     2401 gagcacgcgg gcgcccgccc ccaccaacgg accgaggaac acgaaggccg tcggatccgg
     2461 cacctggtag ccggcgacca ggatctgggc ggtcgcgccg gtgccctgga cgatgccggg
     2521 gttggccgcg ccgtagaggt tgcccatcag caggccgaac tgggccgaga ggccggagaa
     2581 cgtaccgaag gtcatgatgt acagcagggt catccaccag gtgtcctggt tggcgaagat
     2641 gtcgaactgc tgggagatgt tggccttgat cggcaccgac ttcaggtact gccacgcgat
     2701 ccaagcgccg atcaggatga acggcagcca gacgaaggac gcagcctggt accagacttc
     2761 ccgatcaggt ttgccggcga tcttcatcat ctggctctgg cccatccacc acagcatgcc
     2821 gaggccgatc agccacgggg tgaggagctg aaccagcgaa accccgaagt tgccga
//
LOCUS       DAOPRQ010000149         2754 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_29303_length_2754_cov_10.771026, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000149 DAOPRQ010000000
VERSION     DAOPRQ010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 2754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2754
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29303_length_2754_cov_10.771026"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /locus_tag="PKJ61_12240"
     CDS             complement(<1..793)
                     /locus_tag="PKJ61_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015071258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOC14630.1"
                     /translation="MAHIDAGKTTTTERILFYTGINYKIGEVHDGAATMDWMEQEQER
                     GITITSAATTCFWKDTQINIIDTPGHVDFTIEVERSLRVLDGAVTVFDGVAGVEPQSQ
                     TVWRQATRYGVPRICYVNKLDRTGASFEFCVKTIRERLNAIAAVLQLPIGAEANFQGV
                     VDLVGMRALMWRGETQIGEDYTIEEIPADMRDAAEVAREELVHLLVDHDDDFAEAWME
                     AEETGAELTVAQIKAAIRRAVLANKITAVICGTSFKNKGVQPMLDA"
     gene            complement(945..1415)
                     /gene="rpsG"
                     /locus_tag="PKJ61_12245"
     CDS             complement(945..1415)
                     /gene="rpsG"
                     /locus_tag="PKJ61_12245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006347241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HOC14631.1"
                     /translation="MPRKGPAPKRPVVVDPVYGSPLVSQLVSKILLDGKKTTAQSIVY
                     DALEGTRTKTGVDPVQTLKKALDNIRPALEVKSRRVGGATYQVPVEVKPGRANTLALR
                     WLVSFSRQRREKTMTERLMNELLDASNGLGASVKRREDTHKMAEANRAFAHYRW"
     gene            complement(1415..1786)
                     /gene="rpsL"
                     /locus_tag="PKJ61_12250"
     CDS             complement(1415..1786)
                     /gene="rpsL"
                     /locus_tag="PKJ61_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020488528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HOC14632.1"
                     /translation="MPTIQQLVRKGRTDKVSKNKTPALKGSPQRRGVCTRVYTTTPKK
                     PNSALRKVARVRLSSGVEVTAYIPGVGHNLQEHSMVLVRGGRVKDLPGVRYKIIRGSL
                     DTQGVKNRKQARSRYGAKKEK"
     gene            complement(1948..2406)
                     /gene="rpiB"
                     /locus_tag="PKJ61_12255"
     CDS             complement(1948..2406)
                     /gene="rpiB"
                     /locus_tag="PKJ61_12255"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013162119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HOC14633.1"
                     /translation="MRIVVASDHAGVDLRQEIADEARSLGHEVTDLGPAPGESLDYPI
                     NGAKVGRLIAAGEYDRGLLVCGTGVGISLAANKVRGVRAVVCSEPYTAVLSRQHNDAN
                     VLALGARVVGTGLARMIVREWLAAEFEGGRHARRVGLIEELGEPAQSPAF"
     gene            complement(2494..>2754)
                     /locus_tag="PKJ61_12260"
     CDS             complement(2494..>2754)
                     /locus_tag="PKJ61_12260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14634.1"
                     /translation="AFAATVALPTDLPDGEHNVVVAYQGNELVRVPVQVLAPVADSFV
                     EALTVGFAGQNDELLAGLAILGGLVVAGGVTILVTSGARRRA"
BASE COUNT          433 a          914 c          907 g          500 t
ORIGIN      
        1 ccgcgtccag catcggctgc acgcccttgt tcttgaacga cgtgccgcag atcacggcgg
       61 tgatcttgtt ggccagaacg gcgcgacgga tcgctgcctt gatctgggcg accgtcagct
      121 cggctccggt ctcctcggcc tccatccagg cctcggcgaa gtcgtcgtcg tggtcgacca
      181 ggagatggac gagttcctcg cgagcgacct cggcggcatc gcgcatgtcg gccgggatct
      241 cctcgatcgt gtagtcctca ccgatctggg tctcgccgcg ccacatcaac gcgcgcatcc
      301 cgaccagatc gaccacaccc tggaagttgg cctcagcacc gatcggcaac tggagcactg
      361 cggcgatcgc gttgagccgc tcgcggatgg tcttgacgca gaactcgaac gacgcaccgg
      421 tgcggtcgag cttgttcacg tagcagatgc ggggcacgcc gtagcgggtg gcctgccgcc
      481 acacggtctg ggactgcggc tccacgccgg caaccccgtc gaacacggtc accgcaccgt
      541 ccagcacgcg cagcgagcgc tccacctcaa tggtgaagtc aacgtggcca ggggtgtcga
      601 tgatgttgat ctgggtgtct ttccagaaac aggtcgtcgc agcagacgtg atcgtgatgc
      661 cgcgctcctg ctcctgctcc atccagtcca tcgtcgcggc gccgtcgtgc acctcgccga
      721 tcttgtagtt gatcccggtg tagaacagga tccgctcggt ggtggtggtc ttgccggcgt
      781 cgatgtgggc catgatgccg atattgcgaa ccttggccag gtcctgcttc aagtcaatgg
      841 ccactagggc tcaaccttcc gtaatcgttc tgccccggct tagtcagggc acagggcacg
      901 gtggtgagac cgtgccctgt tctgggttca aatcgtgctg gggatcacca gcggtaatgg
      961 gcgaaggcgc ggttggcctc ggccatcttg tgggtgtcct cgcgacgctt caccgaagcg
     1021 ccgaggccgt tggacgcgtc cagcagttcg ttcatcaacc gttcggtcat ggtcttctcg
     1081 cgacgctgcc gggagaagct caccagccag cgcagtgcca gggtgttggc ccggccgggc
     1141 ttgacctcga ccggcacctg gtaggtggca ccaccgacgc ggcggctctt cacctcgagc
     1201 gcgggacgga tgttgtccag cgccttcttc agcgtctgca cggggtctac accggtcttg
     1261 gtgcgagtgc cttcgagggc gtcgtagacg atgctctgcg cggtggtctt cttgccgtcc
     1321 agcaggatct tgctgacgag ctgcgaaacc agcggcgagc cgtagaccgg atcgacgacg
     1381 accggacgct tcggggcggg acccttgcga ggcattactt ctccttcttc gcgccgtaac
     1441 ggctgcgggc ctgcttgcgg ttcttgacac cctgggtgtc cagcgagccg cggatgatct
     1501 tgtagcggac gcccgggagg tccttcactc gaccgccgcg gaccagcacc atcgagtgct
     1561 cctgcaggtt gtggccaacg cccggaatgt aagcagtgac ctcgacgccc gaggagagcc
     1621 ggacgcgagc gaccttgcgc agcgcggagt tcggcttctt gggggtggtg gtgtacaccc
     1681 gggtgcagac gccgcgacgc tggggcgaac ccttcagcgc aggggtcttg ttcttgctga
     1741 ccttgtcagt gcggcccttg cggaccaact gctggatagt gggcaccggc tggtatctct
     1801 ttccgttact tgtgtgtgtg gcgcgcgccg agcgccaccg gtcgatccct gagctagtca
     1861 aagggtccgg gccggacgcg gcgcacgcac gcatggttcg gtcacccgaa cacagcgctc
     1921 aaggctactt gatcgttgcg ggggcggtca aaacgcgggg ctctgggccg gttcgcccag
     1981 ttcctcgatg agacccaccc tgcgggcatg ccgaccaccc tcgaactcgg cggccagcca
     2041 ttcgcggacg atcatccggg cgagaccggt gccgaccacg cgggcgccga gcgcaagcac
     2101 gttggcatcg ttgtgctgcc gggagagcac cgcggtgtag ggctcagagc agaccaccgc
     2161 gcgcacgccg cgcaccttgt tcgctgccag cgagatgccc accccggtgc cacagaccaa
     2221 caggccgcgg tcgtactctc cggcggctat caggcgtccg accttcgcgc cgttgatcgg
     2281 gtagtccagc gactcccccg gcgcgggtcc gaggtcggtg acctcgtgac cgagcgagcg
     2341 cgcctcgtcg gcgatctcct gccgaaggtc cactccggcg tgatcggaag cgaccacgat
     2401 tcgcatgtct gccgtccttt gccttggttc agccctaccc agcgcgaacc ccgcgcggac
     2461 gtcgaacggc agccgcgcgg ggttcgagaa aagctaggcg cgacgtcgag cgccgctggt
     2521 gaccagaata gtcactccgc ccgcgacgac gagaccgccg aggatcgcca gaccggccag
     2581 cagttcgtcg ttctgccccg cgaacccgac ggtgagcgcc tcgacgaaac tgtccgcgac
     2641 cggagcaagg acctggaccg gcacccggac gagttcgttg ccctggtagg ccactacgac
     2701 attgtgctca ccgtccggca ggtccgtcgg tagcgcgacg gtcgccgcga aagc
//
LOCUS       DAOPRQ010000150         2703 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_30028_length_2703_cov_14.433535, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000150 DAOPRQ010000000
VERSION     DAOPRQ010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30028_length_2703_cov_14.433535"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            224..424
                     /locus_tag="PKJ61_12265"
     CDS             224..424
                     /locus_tag="PKJ61_12265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14635.1"
                     /translation="MRRTIVARRPAGKRAFGEMSKLPSGKYRARYTGPDLRRYSAPVT
                     FVAKMDAEAWLVSQERLLSRGE"
     gene            455..1450
                     /locus_tag="PKJ61_12270"
     CDS             455..1450
                     /locus_tag="PKJ61_12270"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOC14636.1"
                     /translation="MPTTVDGYAVVALGRRRLRPATVALYDRLLRLAILPAFGGRELR
                     DITSAEVTAWYGSMKRTPTQQANAYGLLKSILKDAVDDGLIVANPCRVKAGAQKTRAR
                     EIEVLTVRQLEAYLAALPENRRVPLLLAGWCGLRSGEVRGLRVRDLDLDAGVVHVRHA
                     VVRVTGQLIIGPPKTAAGIRTVAIPPHLMPVLRVWLTAQPPRKRDALLFPAGDGVSPL
                     NDSVLRDAHDKAKAAIGVPGLTIHALRHTSATLAAQLGATLAELQARIGHSTANMAMR
                     YQHVAAERDAQLAARMSALAAAQTRAKIPSRLQVTAKQPAIPSVGDSRESDNRDK"
     gene            1983..2672
                     /locus_tag="PKJ61_12275"
     CDS             1983..2672
                     /locus_tag="PKJ61_12275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14637.1"
                     /translation="MTLEDVDAEVGKGSEFTFAGAATTEVHQLKRQNGNSNSWSVKAL
                     ADHNVFEAATGHVAAGREFHFVSLVPCRPLQELAERARKSTDLVEFTQSWLTNELQGV
                     FSQLAADAFLGSPQKAWDTLRGMWFKVQDEGDIVRMNAVLAGCQLGGATGELVSLAIG
                     DILLENLGRRLTRVELLELLEAKAIHPLTSDSRRSASEQLRAVTNSWRESVKRELLQP
                     RIERTLSDRLV"
BASE COUNT          473 a          867 c          910 g          453 t
ORIGIN      
        1 ctgactaggg gttttcctct gcccgcagtt ggactgcggt ggtcacagat gggcaagaaa
       61 gccctttcat tccccacgta ttccccacga cgagccgtcc ctggggaatg ggtgctagct
      121 ggggtgctag ctgagctagc gattcgacct cttcgcggct cgactggact gctagatcgg
      181 ggacgtccgg gaggtacctc tcgcgttcgg tattgggtca tggatgaggc ggacgatcgt
      241 ggctaggcgt ccggcgggca agcgggcgtt cggggagatg agcaagttgc cgtcggggaa
      301 gtaccgggcg cggtacaccg gtccggacct gcgccggtac agcgccccgg tgacgttcgt
      361 ggccaagatg gacgccgagg cgtggctggt cagccaggag cggctgctgt cgcgcggcga
      421 gtgagagccg ccccgcaagt tggcggctcc ggcggtgccg acgactgtgg acgggtacgc
      481 cgtggtggcg ctgggacgcc gcagattgcg gccggcgacg gtcgcgttgt acgacaggct
      541 gctgcggttg gcgatcctgc cggcgttcgg tggacgcgaa ctgcgcgaca tcacctcggc
      601 tgaggtcacg gcctggtacg ggtcgatgaa gcggacgccc acccagcagg ccaacgccta
      661 cgggctgctc aagtcgatcc tcaaggacgc cgtcgacgac ggtctgatcg tcgccaaccc
      721 gtgccgggtc aaggccggcg cccagaagac ccgagcccgc gagatcgagg tgctgacggt
      781 ccgccagctg gaggcatacc tggccgccct cccggagaac cggagggtgc cgctgctgct
      841 ggccggctgg tgcggactcc ggtccggcga ggtccgcggg ctccgcgtcc gagacctcga
      901 tctggacgcc ggcgtcgtcc acgtccgcca tgccgtcgtc cgggtcaccg gacagctcat
      961 catcggacca cccaagaccg ccgccggcat ccgcacggtc gcgatcccgc cacatctgat
     1021 gccagtgctg cgggtctggc tgaccgcaca gccgccccgc aagagggacg cgctgctgtt
     1081 cccggccggt gacggtgtgt cgccgctgaa cgactcggtg ctgcgggacg cccatgacaa
     1141 ggcgaaggcg gcaatcggcg tgcctgggct caccatccat gctctgcggc acacgagcgc
     1201 caccctggcc gcccagctcg gcgcgacgct ggccgagctg caggcccgga tcgggcacag
     1261 caccgccaac atggccatgc gctaccaaca cgtcgccgcc gaacgagacg cccaactcgc
     1321 cgcccgaatg tcggccctag cggcggcgca gactcgcgcc aagatcccgt cgaggctcca
     1381 ggtgacagcc aagcagcccg cgatccccag tgtcggggat agtcgggaga gcgacaaccg
     1441 ggacaaatga ctcaccccgc agcaacgtag tgcgctcgac caagacgact tcgcatctct
     1501 cgatagggtc ccgtcatgag gccgtcgccg caggaagtcg cgtcccagac agcgaccatt
     1561 cttcgatcac cgagcccaag gcgtatccac gatgggcttc catggctgcg ttccccgggc
     1621 atcgcccctg caaccgcagg agtaccagcc gcagcgggca ttggtcgctc ctggcacatg
     1681 gccttgatgc ccggatggga taaggcccgg gcagggtgga gcacgcacct cgagcgtttc
     1741 gcacacgtct tctggttcct atgggacgac catgactgga acgacttgtg cgacgctgcc
     1801 accaagttgg cccggcagat caccgccgtc cacggcgatg atgcgattga gccaagcgcc
     1861 tgcagcctgg agccaacgtg agtccaaggc caggcgggga agcggacaag cttggaaacc
     1921 ggtacgaact cgcctgggcc gttcgacatg ccctctactg tctgactgac ggcagccact
     1981 cagtgacgct tgaagatgtt gacgccgagg ttggtaaggg ctccgagttc actttcgcgg
     2041 gcgccgcgac caccgaggtc catcagctca agcgtcagaa cggcaacagc aacagctggt
     2101 ctgtgaaagc gctggctgac cacaacgtgt tcgaggcggc tactggacat gtggctgccg
     2161 gtcgcgagtt tcacttcgtc tcgcttgtcc catgtcggcc gctgcaagag ctggctgaac
     2221 gtgcgcggaa gtcgactgat cttgttgagt tcacccagtc ttggctgaca aacgaactcc
     2281 agggtgtgtt tagtcagcta gccgcggatg cgttcctagg tagtccacag aaggcgtggg
     2341 acaccctgcg cgggatgtgg ttcaaggtcc aggacgaagg cgacatcgtg cggatgaacg
     2401 ccgtgctggc tggatgccag ttgggcggcg ccactgggga actcgtctcg ctcgctattg
     2461 gtgacatcct tcttgagaac ctgggccgtc ggctgacccg tgtcgaactg ttggagctac
     2521 ttgaggccaa ggcgattcac ccgctgacgt ccgactcgcg ccgaagcgcg agcgagcagc
     2581 ttcgcgcagt gactaacagt tggcgcgagt ctgtcaaacg tgaactgctg cagccgcgca
     2641 tcgagcgcac cctatccgac agactcgtgt gagacgcccg agttgatcgg aacttggctg
     2701 ggg
//
LOCUS       DAOPRQ010000151         2677 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_30385_length_2677_cov_12.131960, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000151 DAOPRQ010000000
VERSION     DAOPRQ010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 2677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2677
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30385_length_2677_cov_12.131960"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..897
                     /locus_tag="PKJ61_12280"
     CDS             <1..897
                     /locus_tag="PKJ61_12280"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020392982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HOC14638.1"
                     /translation="FREPIIISNIPRLVPGWTKPIVIGRHAHGDQYKAANFKVPGAGK
                     VTITFTPEDGSEPIEHVVANFPESGGVAMGMYNFNESIRDFARASFSYGLLRNYPVYL
                     STKNTILKAYDGAFKDIFAEVFEAEFAAEFASRGLTYEHRLIDDMVASAMKWEGGYVW
                     ACKNYDGDVQSDTVAQGFGSLGLMTSVLMTPDGKTVEAEAAHGTVTRHYRMHQQGKPT
                     STNPIASIFAWTGGLKHRGKLDGTPEVTAFAETLEKVCIETVESGKMTKDLALLVGPE
                     QAWLTTEDFLAALDENLAAKLG"
     gene            1027..2064
                     /locus_tag="PKJ61_12285"
     CDS             1027..2064
                     /locus_tag="PKJ61_12285"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOC14639.1"
                     /translation="MTHASLPEDVAVSYVMPVLNEAGHLAEAVGAVLAQEYAGEQELV
                     LALGASTDATDAVAAELAAADPRVRLVSNPRNDIPIGLNLAIAASRHPVVIRVDAHAE
                     LPAGYTASAVEMLRRTGAANVGGVMVARGHGRFQQAVARAYNSPFGLGGGSWHHGEDE
                     AECDSAYLGVFRREVLAEVGGYDETLRRAEDWELNHRIRLAGYSIRFTPRLRVTYWPR
                     DNWDALRRQMYATGVWRGHLVRRQASTPWRYLPPPVVAVLVAISLAVGLLQVFGVGRG
                     AGWALAHVPSLGYLAGVTAVGLTRLGGDGLVDRLLNVVVLMTMHLAWGAGFIRGWLFG
                     AERTVDRSRVK"
     gene            complement(2207..>2677)
                     /locus_tag="PKJ61_12290"
     CDS             complement(2207..>2677)
                     /locus_tag="PKJ61_12290"
                     /inference="COORDINATES: protein motif:HMM:NF016357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HOC14640.1"
                     /translation="DLVLLIRPHPLGVGDYTFGHDRVRLITPAEQPESMPLLWGLDAL
                     VTDYSSILVDYAVTGRPILLLAPDLEHYRATRGLYVDYDWLSGGAWSTDWDQVLDRLE
                     SVLTLPEAAMVTSAHSRQLAEVFHEWTDGGSAARVADAVAKLAGTTPRGRHGIR"
BASE COUNT          405 a          876 c          964 g          432 t
ORIGIN      
        1 ttccgcgagc cgatcatcat ctccaacatc ccgcggctgg tgccgggctg gaccaagcca
       61 atcgtgatcg ggcgccacgc ccacggcgac cagtacaagg cggccaactt caaggttccc
      121 ggcgctggca aggtgaccat caccttcacc cccgaggacg gttcggagcc gatcgagcac
      181 gtcgtcgcca acttcccgga atccgggggc gtggcgatgg gcatgtacaa cttcaacgag
      241 tcgatccgcg acttcgcccg ggcgtccttc tcctacgggc tgctgcgcaa ctacccggtc
      301 tacctgtcca ccaagaacac gatcctcaag gcctacgacg gggcgttcaa ggacatcttc
      361 gccgaggtct tcgaggccga gttcgccgct gagttcgcgt cccgcgggct aacctacgag
      421 caccggctga tcgacgacat ggtcgcgtcc gcgatgaagt gggagggcgg ctacgtctgg
      481 gcctgcaaga actacgacgg cgatgtgcag tccgacaccg tggcgcaggg cttcggctcg
      541 ctcggcctga tgacttcggt gctgatgacc ccggacggga agacggtcga ggccgaggct
      601 gcgcacggga cggtgacccg gcactaccgg atgcaccagc agggcaagcc gacttcgacg
      661 aacccgatcg cgtccatctt cgcctggacc ggtggcctca agcaccgcgg caagctggac
      721 ggcacgcccg aggtgacggc cttcgccgag acgctggaga aggtctgcat cgagaccgtc
      781 gagtcgggga agatgaccaa ggacctggcg ctgctggtcg gcccggagca ggcgtggctc
      841 accaccgagg acttcctcgc tgccctggac gagaacctgg ccgccaagct cggctgacct
      901 cgtccgcgcg cacccatcgt cgagggccag cctccgtcgc cgagcgccag caccaggagc
      961 gggctctcga tggcggtggc tggccctcga cgatctggga cggggaggtc tgcgagaatg
     1021 ggcgtcgtga cgcacgcttc gctgcccgag gatgtggcgg tctcctatgt gatgccggtg
     1081 ctcaatgagg ccggccatct ggccgaggcg gttggcgcgg tgctcgccca ggagtacgcc
     1141 ggcgaacagg aactggtgct ggcgctgggt gcgtccaccg atgcgacgga cgcggtcgcg
     1201 gccgaactcg cagccgccga tccgcgcgtc cgactggttt cgaacccgcg gaacgacatc
     1261 ccgatcggac tcaacctggc aatcgcggcc agccggcatc cggtggtgat ccgcgtggac
     1321 gcgcacgctg aactgccggc cggctacacc gcgtccgccg tggagatgct gcggcgcacc
     1381 ggtgcggcga acgtcggcgg cgtcatggtc gccaggggcc acggacgctt ccagcaggcg
     1441 gtggctcggg cctataactc cccgttcggg ctgggtggcg gttcgtggca ccacggcgag
     1501 gacgaagcgg aatgcgattc cgcttatctc ggggtgttcc ggcgggaggt gctggccgag
     1561 gttggcggct acgacgagac cctgcgtcgc gccgaggact gggagctgaa ccaccgcatc
     1621 cgactcgccg ggtacagcat ccggttcacc ccgaggctgc gggtcaccta ttggccccgc
     1681 gacaactggg acgcgttgcg ccgccagatg tatgcgaccg gggtctggcg cggacacctg
     1741 gtgcggcggc aggcgtccac gccgtggcgg tacctgccgc cgccggtggt cgcggtattg
     1801 gtcgcgatca gcctggcggt cgggttgctg caggtgttcg gggtggggcg cggtgccggc
     1861 tgggcgttgg cgcacgtccc gtcgctgggc tacttggccg gcgtgactgc ggtcggcctg
     1921 actcggctgg gtggcgatgg tctggtcgat cggctgctga acgtggtcgt gttgatgacc
     1981 atgcatctcg cctggggcgc cggcttcatt cgcggctggc tattcggtgc ggagcggacg
     2041 gtggatcgct cccgggtcaa gtaagcgatc tctggtcagg ctcagtgagc ggtggctgag
     2101 cttgacgaag cccggggtag gcgacccttc ggtacagctg gctcgtccct cgccagcctc
     2161 ccagggacgg tttgccagac cccttgtcag gctgcgggat cgggtgctag cgaatcccgt
     2221 ggcgtccgcg cggggtcgtg ccggcgagtt tggcgacggc gtccgctacc cgggcggcgc
     2281 tgccgccgtc cgtccactcg tggaagacct cggccaactg ccggctgtgc gcggacgtga
     2341 ccatcgctgc ctcggggagg gtgagcaccg actccagccg gtcgagtacc tggtcccagt
     2401 cggtgctcca ggcgccgccg gacagccagt cgtagtcgac atagaggccg cgggtcgccc
     2461 ggtaatgctc gaggtcgggc gccagcaaca ggatcggccg cccggtgacc gcgtagtcga
     2521 ccaggatcga cgagtagtcg gtgacgagtg cgtccagccc ccacagcagc ggcatcgact
     2581 cgggctgctc ggcgggcgtg atcagccgca cccggtcgtg gccaaaggtg tagtcgccga
     2641 ccccgagcgg atgcggacgg atcagcagca ccaggtc
//
LOCUS       DAOPRQ010000152         2629 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_31116_length_2629_cov_14.000777, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000152 DAOPRQ010000000
VERSION     DAOPRQ010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31116_length_2629_cov_14.000777"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            80..448
                     /locus_tag="PKJ61_12295"
     CDS             80..448
                     /locus_tag="PKJ61_12295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14641.1"
                     /translation="MRHIPGSGMGSMLAGILGFLGTLTFLSLLALLVFFLIRRAKGQG
                     APAGPKGFHGHHHGPPMPPALQVLDERLARGEIEIDDYVARRAAMLGTLPNENEWTPP
                     AAPQQPPAPPKPPEGNDPQI"
     gene            819..>2629
                     /gene="ileS"
                     /locus_tag="PKJ61_12300"
     CDS             819..>2629
                     /gene="ileS"
                     /locus_tag="PKJ61_12300"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015069369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HOC14642.1"
                     /translation="MTETTRPTYRPVPAMLDLPAMEREILELWATQDTFAASLKRTAG
                     GRPWTFYEGPPTANGMPGTHHIEARVFKDVFPRFKTMQGYHVERKAGWDCHGLPVELA
                     VEKELGFNGKPDIEAFGVEAFNAKCRESVSRHVDAFTDLTTRMGYWVNLDQAYWTMDS
                     AYVESVWWALKQIHTKGLLVEDYRVAPYCPRCGTTLSDHELAQGYEDVTDPSVYVRFP
                     LISGPLADKASLLVWTTTPWTLVSNTAVAVHPDVTYVVATNDEESLVVAEPLAEKVLG
                     EGWQLGRTFTGRELELWKYQRPFELVDFPDAVGGTHYVVLADYVTTEDGTGLVHQAPA
                     FGEDDMFVCRQYGLPMVNPIRPDGSFEADLDLVGGMFFKTADKVLVDDLTQRGLMYRV
                     LNYEHSYPHCWRCHTTLLYYAQPSWYIRTTAIKDELLRENANTSWYPEHIKWGRYGDW
                     LNNNIDWALSRSRYWGTPLPIWRCPAGHQTCVGSRAELSELTGRDYSALDPHRPFVDD
                     ITFACPNCEQVARRVPEVIDAWFDSGSMPFGQWGYPWAEGSVEAFQTAYPADFICEAI
                     DQTRGWFYSLMAVGTLVFDKSSYKNVLCLGHILAEDGR"
BASE COUNT          454 a          929 c          827 g          419 t
ORIGIN      
        1 acaggtccag cacaggtggg cgagtaagca tggacttcgt cggcgtcacc gggacgccac
       61 tgaccttttt ggaggttcga tgcgccacat tccaggatcg ggcatgggct cgatgcttgc
      121 cggcattctc ggattccttg gcacactgac gttcctgagc ttgctggctc tgctggtctt
      181 cttcctgatc cgccgggcaa agggtcaagg tgcgcccgcg ggaccgaagg gcttccacgg
      241 gcaccaccac ggtccgccga tgccgcccgc gttgcaggtg ctggacgaac ggctcgcccg
      301 cggtgaaatc gagatcgacg actacgtggc ccggcgggcc gcgatgctcg gcacgctgcc
      361 caacgagaat gagtggacgc cgccagccgc gccgcagcag cctccggcgc cgcccaagcc
      421 gccggagggc aacgacccgc agatctgatc gccgcccgca atcgagggcc agccaacgct
      481 tttcagcgct ccaccccggg gtcgagcctt gggcgcgaag gctggccctc gatgcgtcac
      541 ggggcatgag ctaccgacgg tcgcagagcg ttcgttacac tgtggaggtc cgcaaggcat
      601 cgatggggcc atcaccccgg agctgccgga agaacagagt gcgtcggccc tcggctggtt
      661 gagcttgtcg aaaccatcga gagtccacaa ctcccactag aaccggccgc ggcgaacctt
      721 gagtggggcg tccagtacgc ccaaggaggg tggtaccgcg ggtccgtcgc aaggcgtcct
      781 cgtcccttcg tccagcatgc cgacgaagga tcgacccgat gaccgaaacc acccgtccca
      841 cgtaccgtcc agtaccggcg atgcttgacc tgccggcgat ggaacgcgag atcctcgaac
      901 tctgggccac ccaggacacc ttcgccgcca gcctcaagcg caccgcgggc ggccgaccct
      961 ggacgttcta cgaaggcccg cccaccgcca acggcatgcc gggcacccac cacatcgagg
     1021 cgcgcgtctt caaggacgtg ttcccgcggt tcaagaccat gcagggctac cacgtcgagc
     1081 gcaaggccgg ctgggactgc cacggcctgc cggtcgaact cgccgtcgag aaggaactcg
     1141 gcttcaacgg taagcccgac atcgaagcct tcggcgtcga ggcgttcaac gcgaagtgcc
     1201 gcgagtcggt ctcccggcac gtggacgcgt tcaccgacct caccacgcgg atgggctact
     1261 gggtgaacct tgaccaggcc tactggacga tggactcggc ctacgtcgag tcggtttggt
     1321 gggcgctgaa gcagatccat accaagggcc tgctggtcga ggattaccgg gtcgccccct
     1381 actgcccgcg ctgcggcacc acgctgagcg accacgagct cgcccagggc tacgaggacg
     1441 tcaccgatcc cagcgtttac gtccgcttcc ccttgatctc ggggccgctc gccgacaagg
     1501 cgtccctgct ggtctggacg accacaccct ggacgctcgt ctcgaacacc gcggtcgcgg
     1561 tgcatcccga cgtgacctat gtcgtggcca ccaatgacga ggaatcgctg gtggtagccg
     1621 agccgctggc cgagaaggtg ctcggcgagg gctggcagct gggccgcacc ttcaccgggc
     1681 gcgaactcga actgtggaag taccagcgtc cgttcgagct ggtcgacttc ccggacgcgg
     1741 ttggcggcac ccactacgtc gtgctggccg actacgtcac caccgaggac ggcaccggac
     1801 tggtccacca ggcacccgcg ttcggcgagg acgacatgtt cgtctgccgc cagtacggtc
     1861 tgccgatggt caacccgatc cgtcccgacg gctccttcga ggctgacctc gacctggtcg
     1921 gcggcatgtt cttcaagacc gccgacaagg tgctcgtcga cgacctcacc cagcgcgggc
     1981 tgatgtaccg ggtgctcaac tacgagcact cctacccgca ctgctggcgc tgccacacca
     2041 cgctgctcta ctacgcccag ccgtcctggt acatccgcac caccgcgatc aaggacgaac
     2101 tgctgcgcga gaacgccaac acctcctggt accccgaaca catcaagtgg ggccgctacg
     2161 gcgactggct gaacaacaac atcgactggg cgctgtcccg gtcgcgctac tggggcacgc
     2221 cgctgccgat ctggcgctgc ccggccggcc accagacctg cgtcggctcg cgcgccgaac
     2281 tttccgagct caccggacgc gactactcag cgctcgaccc gcaccgcccg ttcgtggacg
     2341 acatcacctt cgcctgcccg aactgcgaac aggtggcccg tcgcgtcccc gaggtgatcg
     2401 acgcatggtt cgacagcggc tcgatgccgt tcggccagtg gggctacccg tgggccgagg
     2461 gctcggtcga ggcgttccag accgcctacc cggccgactt catctgcgag gcgatcgacc
     2521 agacccgcgg ctggttctac tcgctgatgg cggtcggcac gctggtgttc gacaagtcgt
     2581 cgtacaagaa cgtgctctgc ctgggccaca tcctcgccga ggacggccg
//
LOCUS       DAOPRQ010000153         2622 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_31214_length_2622_cov_8.940008, whole genome shotgun sequence.
ACCESSION   DAOPRQ010000153 DAOPRQ010000000
VERSION     DAOPRQ010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31214_length_2622_cov_8.940008"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1027)
                     /locus_tag="PKJ61_12305"
     CDS             complement(<1..1027)
                     /locus_tag="PKJ61_12305"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HOC14643.1"
                     /translation="MYAFRDDLATTLSEIRAAGLFKSERQLTTPQSAQVATPSGPVLN
                     FCANNYLGLADHPAVLAAAKTALDEWGFGMASVRFICGTQTQHVELERRLSEFLGTED
                     TILFSSCFDANGGLFEVLFGEADAIISDELNHASLIDGIRLSKAARYRYRNRDLADLE
                     AQLVTAADARRKVIVTDGVFSMDGYFAPLEGICDLAERYGALVVVDDSHAVGFVGTGG
                     RGTPELFGVADRVDVITGTLGKALGGASGGYVSGPSEVIALLRQRARPYLFSNSVAPA
                     VVAGSLAALELVSSSADARDRLNSNAALFRELMTAAGFELLPGAHPIVPVMFPGADGA
                     RQAAAIAD"
     gene            complement(1137..2174)
                     /gene="tdh"
                     /locus_tag="PKJ61_12310"
     CDS             complement(1137..2174)
                     /gene="tdh"
                     /locus_tag="PKJ61_12310"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HOC14644.1"
                     /translation="MRALVKTHAGPGLELRDVPEPNCGPTEVKIRVMRAGLCGTDLHL
                     ESWDDWAASTVRAPLTIGHEFYGEVVEVGAEVTGIALGARASGEGHIVCGRCRNCRAG
                     RRHVCIHTVGLGVNTDGAFADFVVLPASNVWLQPDAIDPDLGAIFDPLGNATHTALQW
                     PVVGEDVLITGAGPIGVMAAAIVRHAGAKHVVVTDLSDYRLGLAAGAGADLCVNVGRT
                     RLADAQHQLGMREGFDIGLEMSGAPSAVAEMLANLNHGAKVAMLGLPKDPYPIDWGRV
                     ITHMITIKGIYGREMFETWYLMSSMLATSPVFADAVRSVITHRFPASDWAEAFAIART
                     GECGKVILDWS"
     gene            complement(2346..>2622)
                     /locus_tag="PKJ61_12315"
     CDS             complement(2346..>2622)
                     /locus_tag="PKJ61_12315"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HOC14645.1"
                     /translation="YLGYKSGTTGVTFGRSIAKTKLISEETGQPIGSTNGIIRQFAHI
                     VDSVICYIGWLFPLWDAKRQTIADKIMKTVVVDNSTDPNAGNYNWNL"
BASE COUNT          445 a          925 c          858 g          394 t
ORIGIN      
        1 ggtcggcgat ggccgcggcc tgccgggcac cgtccgcgcc cgggaacatc accggcacga
       61 ttgggtgcgc accgggcagc agctcgaacc cagccgcggt catcagctcg cggaacaagg
      121 ccgcattcga gttgagccgg tcacgggcgt ccgccgacga gctgaccagc tccaacgcgg
      181 ccagcgagcc ggccaccacc gccggcgcca ccgagttcga gaagaggtac ggacgcgccc
      241 gctgccgcag gagcgcgatc acctcggaag gtccgctcac atagccaccg gacgcaccgc
      301 cgagcgcttt ccccagagtg ccggtgatca cgtccacccg gtcggcgacg ccgaacaact
      361 ccggcgtccc tcgcccaccg gtgccgacga aaccgaccgc gtgcgaatca tccacgacca
      421 ccagagcgcc gtagcgctcg gcgaggtcgc agatgccctc caggggcgcg aaatagccgt
      481 ccatcgagaa cacgccgtcg gtgacgatca ctttgcgacg ggcatcggca gcggtcacca
      541 gttgggcttc caggtcggcc aggtcacggt tgcgatagcg gtagcgcgcg gccttcgaca
      601 gcctgatgcc atcgatcagc gaagcgtggt tcagctcgtc ggagatgatc gcgtccgcct
      661 cgccgaagag cacctcgaag agcccgccgt tggcgtcgaa acagctcgag aacagaatgg
      721 tgtcctcggt gccgagaaac tcgctcagcc ggcgctccag ctcgacgtgc tgggtctgcg
      781 tcccgcagat gaagcggacg gacgccatcc cgaagcccca ctcgtccagc gcggtctttg
      841 cggccgcgag caccgccggg tggtcggcca gccccaggta gttgttggcg cagaagttga
      901 gcaccggccc ggacggtgtg gccacctgcg ccgactgcgg ggtggtcagc tgccgctcgc
      961 tcttgaagag cccggccgct cgaatctcgc tcagggtggt ggccaggtca tcgcggaagg
     1021 cgtacatgcc gtcaccctat tcccgcaagc gcccaagagc aggagccgat ccatgccctc
     1081 gatgcactcc agcgcccgat ttccggcccc agcgaccatc cagggccgaa atcagctcac
     1141 gaccagtcga ggatcacctt tccgcactcc ccagtgcgag cgatcgcgaa agcctcggcc
     1201 cagtcggagg cggggaagcg gtgggtgatc accgagcgga cggcgtcggc gaacaccggc
     1261 gaggtggcca gcatcgagct catcaggtac caggtctcga acatttcccg tccgtagatc
     1321 cccttgatgg tgatcatgtg ggtgatcacc cggccccaat cgatcgggta cgggtccttc
     1381 ggcaggccga gcatggccac cttggcgccg tggttgaggt tggccagcat ctccgcgacc
     1441 gccgacggcg ccccggacat ctccagtccg atgtcgaaac cctcccgcat accaagctgg
     1501 tgctgcgcgt cggcgagccg ggtgcggcca acgttcacgc acaggtcggc cccggcgccc
     1561 gcggccaggc ccagccggta gtcggacaga tcggtgacca ccacgtgctt ggccccggca
     1621 tggcggacga tcgccgcggc catcactccg atcggtccgg cgccggtgat cagcacgtcc
     1681 tcccccacca ccggccactg cagcgcggtg tgggtggcat tgccgagcgg gtcgaagatc
     1741 gcccccagat cggggtcgat cgcgtccggt tgcagccaga cattggacgc cggcagcacc
     1801 acgaagtcgg cgaaggcacc gtcggtgttc accccgagcc cgacggtgtg gatgcagacg
     1861 tggcgccgtc cggcccggca gttgcggcag cgtccgcaga cgatgtggcc ctccccggac
     1921 gcccgcgcac ccagcgcgat cccggtcacc tcggcgccga cctcgaccac ctcgccgtag
     1981 aactcgtggc cgatggtcag cggagcgcgg acggtggaag ccgcccagtc gtcccacgat
     2041 tccaggtgca ggtcggtgcc gcacaggccg gcccgcatca cccggatctt cacctcggtc
     2101 ggcccgcagt tcggctccgg aacgtcacgg agttccagtc ccggtccggc gtgtgtcttc
     2161 accagtgcgc gcataccgag cacgctatcg cccgtccgcg cagcccagct cgctgcacga
     2221 ccgatgcacg atcggggaga aaggaaccgc cagcgcgcta gtgaaacgtc aaggtccacc
     2281 gcctgacgca gcaagggccg gccgggatca ctcccgaccg gccccgctca cgtcctaccg
     2341 ggcgactaca ggttccagtt gtagttgccg gcgttcggat cggtgctgtt gtcgacgacc
     2401 acggtcttca tgatcttgtc ggcgatggtc tgacgcttgg cgtcccacag cgggaacagc
     2461 caaccgatgt agcagatgac cgagtcgacg atgtgggcga actgacggat gatgccgttg
     2521 gttgaaccga tcggctgccc ggtctcctcg ctgatcagct tggtcttggc gatgctgcgg
     2581 ccgaaggtga ccccggtggt gccgctcttg tagcccaggt ag
//
LOCUS       DAOPRQ010000154         2547 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_32343_length_2547_cov_12.483146, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000154 DAOPRQ010000000
VERSION     DAOPRQ010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32343_length_2547_cov_12.483146"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..95)
                     /locus_tag="PKJ61_12320"
     tRNA            complement(2..95)
                     /locus_tag="PKJ61_12320"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(57..59),aa:Ser,seq:gct)
     gene            217..1839
                     /locus_tag="PKJ61_12325"
     CDS             217..1839
                     /locus_tag="PKJ61_12325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HOC14646.1"
                     /translation="MTAAHESPSAPRRIADSEPLGGGGSARRVVDERTFGSAVGWTVL
                     GTLIPGLGLWRAGRKVAGGILMGLFALVIGGGIALVLTQRKQLSNLALAPNVLEGVAV
                     GLAVVAVGYVLLIGFTHLCLRPARPTTGQRLAGAGVVGLLSFVVAAPMAVGSTYAWTT
                     ANMVNNMFESEDPSSTQSPVDVQDPWADQDRINFLIIGGDSGNDRSSKLGDRTDTVIV
                     ASIDTHSGGTTLFTLPRNTARMPFPSDSPLAKYYPNGFYDGVNPANAEYFLNAMYRNV
                     PGRVTDHSALGKTKDFGAEVMKASVGEALGLDIKYYVKVNMDGFKDFINAIGGVTLNV
                     NYEIPIGGQTDAGIKPDGYIKVGPNQHMNGRLALWYARGRYGLTDYNRMERQRCVINA
                     VVQQTTPLDVLQNYQSIAAAGEKTITTDVKRSLLPALVDLASKVQGTKLRSVVFTPET
                     GFISANPDWDKVQSRVKKALKETEKAAQPTASATTTPNGTASASAASTEPTSSATTSA
                     TPSSSASSSSASSTAKAKSEDLEDVCGYHPEK"
     gene            complement(1951..2418)
                     /locus_tag="PKJ61_12330"
     CDS             complement(1951..2418)
                     /locus_tag="PKJ61_12330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14647.1"
                     /translation="MRQRPPLRALALASALMVIGLVLMLMSHLLGWHLALALIGTLAL
                     LLGVGLLTASWAMAHSMSVEVVLDEEGYRVYGGGTDETGRWADISRVTIADGLLTLYR
                     LDGSTLRLVVSRSSVADLDALGADIARHLDADRGYQQPTNLSRLTDTELPNEH"
     gene            complement(2463..2547)
                     /locus_tag="PKJ61_12335"
     tRNA            complement(2463..2547)
                     /locus_tag="PKJ61_12335"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2511..2513),aa:Ser,seq:tga)
BASE COUNT          477 a          932 c          753 g          385 t
ORIGIN      
        1 atggcggagg tgcggggatt tgaaccccgg agagggttga agccctcaac ccgcttagca
       61 ggcgggcgcc atagaccgga ctaggcgaca cctccaagac cgtccgccag cctacaaggc
      121 gtccgagtgc ggcggcaaag cggagcgtga atccggcttg ctgcagcccg gtaacctgtt
      181 agtcggccct cgttctccca aaaggaaccc ttcagtatga ctgctgccca cgaatcgccc
      241 agcgcaccgc gccgtatcgc cgattccgaa cccctcggcg gcggcggttc ggccaggcga
      301 gtggtggacg agcggacctt cggttccgcg gtcggctgga cggtccttgg caccctgatt
      361 cccggtctcg gcctctggcg cgccggacgc aaagtcgcag gcggcatcct gatgggcctg
      421 ttcgccctcg tgatcggcgg cggcatcgcg ctggtactca cccagcgcaa gcaactcagc
      481 aacctcgcgc tggcaccgaa cgtgttggaa ggcgtcgccg tcgggctcgc cgtcgtcgcg
      541 gtcggctacg tcctcctgat cggcttcacc cacctctgcc tgcgtcccgc gcgtccaacc
      601 accggacagc gcctcgccgg cgccggcgtc gtcggcctgc tctccttcgt cgtcgccgca
      661 cccatggccg tgggctccac ctacgcctgg acgaccgcca acatggtcaa caacatgttc
      721 gagtccgagg atccgagcag cacgcaatca ccggtcgacg ttcaggaccc ctgggccgac
      781 caggaccgga tcaacttcct catcatcggc ggcgactccg gcaatgaccg gtccagcaag
      841 ctcggcgacc gtaccgacac cgtgatcgtt gcctccatcg acactcactc gggcggcacc
      901 acgctgttca ccctgccgcg caacaccgcg cgcatgccct tcccgtccga ctccccgctc
      961 gccaagtact acccgaacgg cttctacgac ggtgtgaatc cggccaacgc cgagtacttc
     1021 ctcaacgcca tgtaccgcaa cgtgcccgga cgcgtcacgg atcacagcgc cctcggcaag
     1081 accaaggact tcggcgccga agtcatgaag gcctccgtcg gcgaggctct cggtctggac
     1141 atcaagtact acgtcaaagt caacatggac ggattcaagg acttcatcaa cgccatcggc
     1201 ggcgtcacgc tgaacgtcaa ctacgagatc ccgatcggcg gtcagacgga cgccggcatc
     1261 aagcccgacg gctacatcaa ggtcggcccc aaccaacaca tgaacggccg gctcgccctc
     1321 tggtacgccc gcggccgcta cggcctcacc gactacaacc gcatggaacg ccagcgctgc
     1381 gtcatcaacg ccgtggtgca gcagaccact ccgctcgacg tcctgcagaa ctaccagtcc
     1441 atcgccgcgg ccggcgagaa gaccatcacc accgacgtca agcgctcact cctgcccgct
     1501 ctcgtcgacc tggccagcaa agtacagggc accaagctgc gcagcgtggt gttcactccg
     1561 gaaacaggct tcatcagcgc caatcccgac tgggacaagg tgcagagccg ggtgaagaag
     1621 gcactcaagg agacggagaa ggccgcgcag cccaccgcct cggcaaccac gacacccaac
     1681 ggcaccgctt ccgcgtcagc cgccagcact gagcccactt cgagcgcgac gacctcggcc
     1741 acgccgtcca gcagcgcgtc ttcgagcagc gcgtcgtcta ccgccaaggc caagagcgaa
     1801 gacctagagg acgtctgcgg ctaccacccc gagaagtagc gctcgaatcc tcgagctggg
     1861 cgggatgccg tccgcggtct caccttcgca agctcagcac aaggcaggcg cgatcagcga
     1921 cccctcgtca agctcaggga tcgctcggtc tcagtgttcg ttgggtagct cagtgtccgt
     1981 aagccggctc aggttcgtcg gttgttgata gccccggtcg gcatccaggt gccgggcgat
     2041 gtcggccccc agagcatcca ggtcggcgac gctgctgcgc gacaccacca ggcgcagcgt
     2101 gcttccgtcc agccgataca gcgtgagcag gccatcggct atggtcacgc ggctgatgtc
     2161 ggcccagcgt ccggtctcgt cggtgccacc gccgtacacc cggtaaccct cctcgtccag
     2221 caccacctcg accgacatcg agtgcgccat cgcccaggaa gccgtgagca accccacgcc
     2281 gagcagcagg gcgagcgtcc cgatcagcgc cagggccaga tgccagccca gcaggtggga
     2341 catcagcatc agcaccagtc cgatcaccat cagggcggac gcgagcgcga gggcacgcag
     2401 cggcggtcgc tgtcgcacat gatgagtcgt cggcacccca cacccctgtt ctgccgttcg
     2461 cgcggcgagg gtgggattcg aacccacgga gccgttaagc tcggccgctt tcaaggcggc
     2521 tgcactagtc cactatgcga cctcgcc
//
LOCUS       DAOPRQ010000155         2478 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_33558_length_2478_cov_14.181180, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000155 DAOPRQ010000000
VERSION     DAOPRQ010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33558_length_2478_cov_14.181180"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1188)
                     /locus_tag="PKJ61_12340"
     CDS             complement(<1..1188)
                     /locus_tag="PKJ61_12340"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HOC14648.1"
                     /translation="MANDLNNLLDRVERVDRSLAAELRAQISTLARRREFGLNFEKHK
                     PETVELYGRTVRAGDKVRLIAPRGQSGPHEDARTWRVQTITKTRTGHQAHLLDLDGND
                     ERDAPLDELVVIADFRDPIYPGLRSTGAVRRGEDKPHHVVINAENFHALEAMLFTSQG
                     AVDAIYIDPPYNTGAKDWKYNNDYVDGEDAYRHSKWLSFMERRLKLARKLLNPNDSVL
                     IVTIDEKEYLRLGLLLQQTFPEATIQMISSVISPSGAARNREFTRVNEFIFFVMIGRA
                     APAKTPDDMLFTERRTEKQNRSPIWNQLRRLGAGPARSDSPSKFYPILLDPTSGAIVG
                     SDAPLPAHMDRATYKPPAGTEATWPTQTDGSDGRWELNRASFETRLENGHVRSTGRGR
                     SGEW"
     gene            complement(1246..2007)
                     /locus_tag="PKJ61_12345"
     CDS             complement(1246..2007)
                     /locus_tag="PKJ61_12345"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOC14649.1"
                     /translation="MRPSGKVRGVNLLARASDSNQASGTLLIAHLDTVRVSPGADDNA
                     SGVAVALQVARLLHQGRHDVTIALVDLEELGQLGSRHLVTVGPRPARVICLESVGYFD
                     DRPGAQRLPVGFGMLFPSLARSIRAREGRGDFLAAVHRGDCEGLLEDLVTTAADQELD
                     LLGLLDRRWNGPGQRFTRWVNPLLMDLDRSDHAPFWRAQVPSILITGTAPLRNSRYHR
                     RGDIPDTLDHRRMNALARSLAHFLATHEPASPDPT"
     gene            complement(2207..>2478)
                     /locus_tag="PKJ61_12350"
     CDS             complement(2207..>2478)
                     /locus_tag="PKJ61_12350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14650.1"
                     /translation="EKFKQQCEMSWKRIDRGRDRDGALGWAGEHATAHPPQSRRVIAA
                     LIGAIARHRAEVLREFGKPNAADAELWDVMAHLGLDPRDYETRDG"
BASE COUNT          365 a          760 c          874 g          479 t
ORIGIN      
        1 ccactctccc gacctacccc tccctgtaga tcgcacatgt ccgttctcca gacgtgtctc
       61 gaatgaggct cggttcaact cccaccgacc gtctgagccg tcagtttgag taggccaagt
      121 tgcttctgtt cccgccggag gcttgtaagt cgcgcggtcc atgtgcgccg gaagcggagc
      181 gtcagagccg acgatggctc cactggtcgg atcaagaagt attggataga acttgctggg
      241 cgagtcgctg cgagctggac ccgcacccag acgccggagt tggttccaga taggcgacct
      301 gttctgcttc tcagtccgcc gctcggtgaa cagcatgtcg tcaggtgtct tggcgggggc
      361 ggctctgccg atcatcacga agaagatgaa ctcgttgact cgcgtgaact ctctgttccg
      421 ggccgccccg ctcggactga tcaccgacga gatcatctgg atggtggctt cagggaaggt
      481 ttgctgtagc aacagcccga ggcggaggta ctccttctcg tcgatggtga cgatcagcac
      541 cgagtcgttg gggttgagca gcttgcgcgc gagcttgagg cggcgctcca tgaaggacag
      601 ccacttgctg tggcgatagg cgtcttcccc gtcgacgtag tcgttgttgt atttccagtc
      661 cttggcgccg gtgttgtagg gcgggtcgat atagatcgcg tcgacggcgc cctggctggt
      721 gaagagcatg gcttcgagcg cgtggaagtt ctccgcgttg atgacgacgt ggtgcggctt
      781 gtcttctcct cggcgcacgg ctccggtgga gcgcagcccg gggtagatcg ggtcgcggaa
      841 gtcggcgatg accacgagct cgtccagtgg tgcgtcccgc tcgtcattac cgtcgagatc
      901 cagcaggtgt gcctggtgcc cggtcctggt cttggtgatg gtctggactc gccaggtgcg
      961 ggcgtcctca tgaggtccgc tctgaccgcg gggggcgatg agccggacct tgtccccagc
     1021 tctgaccgtc cggccgtaca gctcgacggt ctctggcttg tgcttctcga agttgaggcc
     1081 gaactcgcgc cgacgggcga gggtgctgat ctgggcgcgc agctcggccg cgagggacct
     1141 gtcaacccgc tctacgcggt ccagcaggtt gttgaggtcg ttggccatgg ggtggtgagc
     1201 tcccggtgtg gtgggtgagt tctgcccatg accttacgac ccgtctcagg tcggatccgg
     1261 tgaggctggc tcgtgggtcg ccaggaagtg cgccaaggac cgtgccagtg cgttcatccg
     1321 tcgatggtcc aaggtgtcgg ggatatcgcc tcggcggtgg taccggctgt ttcgcagggg
     1381 cgcggtgccg gtgatgagga tggagggcac ttgggcccgc cagaacggtg cgtggtcgct
     1441 gcggtcgaga tccatcagca gggggttcac ccaccgggtg aaccgctgcc cgggtccgtt
     1501 ccaccggcga tcgagcagac cgagcaggtc gagctcttgg tcagcggcgg tcgtgaccag
     1561 atcctcgagg aggccttcgc agtcgcctcg gtgaacggcg gccaggaagt caccccgacc
     1621 ctcccgcgcg cggatggatc gtgcgagtga agggaacagc atcccgaacc cgaccggtag
     1681 gcgctgagcg cctggccggt cgtcgaagta gcccaccgac tcaagacaga tcacccgagc
     1741 aggtcgcgga cccactgtga ccaggtgtcg agacccgagc tgaccgagct cttcgaggtc
     1801 gaccagggcg atggtgacgt cgtgacgtcc ctggtgcagg agccgtgcca cctgcaaggc
     1861 caccgccacg cccgaggcgt tgtcgtcggc gccggggctg acccggacgg tgtcgaggtg
     1921 tgcaatcaga agagtcccgc tggcctggtt gctgtcgctg gctctcgcga gcaggttgac
     1981 cccgcggact ttgcccgagg gtcgcatcgg gagcactgcg cccggcttgc cgtcgtcggg
     2041 gatgcgaagc cggttcgggg cgacgaaggg ctgctcggtg acgtcccagc cagcgtcgat
     2101 gagctgttgg gcgcagtagg tccgagcgcg ctccaggctt ccggctagcg cccggtggcg
     2161 tggttgagac gcgaggtatg cgatatcacc tcggagcctg tgcccgtcat ccgtcacggg
     2221 tctcgtagtc ccgcgggtcc aggccgaggt gggccatgac gtcccacagc tcggcgtcgg
     2281 cggcattggg cttgccgaac tcgcgcagga cctcggcccg gtgcctggcg atcgcgccga
     2341 tgagtgccgc gatgacccgc cgggactgcg gcgggtgggc ggttgcgtgc tcgccggccc
     2401 accccagggc accgtcgcgg tcacggccac ggtcgatccg cttccagctc atctcgcact
     2461 gctgcttgaa cttctcgg
//
LOCUS       DAOPRQ010000156         2456 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_33966_length_2456_cov_11.945856, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000156 DAOPRQ010000000
VERSION     DAOPRQ010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 2456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2456
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33966_length_2456_cov_11.945856"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            41..1105
                     /locus_tag="PKJ61_12355"
     CDS             41..1105
                     /locus_tag="PKJ61_12355"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOC14651.1"
                     /translation="MSAPAHGSVSRTSKASTASAAPVPVRHTGRMPRMPLRTRALAAL
                     LRRGIFPSIATMDLAAIQRSRARRLPSRAPVAWVVGRVSRDLQVSMEWFPTRDGSRRQ
                     ARVYRPAGQPPQGGWPVVCYFHGGGFVLGSITQSDPICSHLASAIPAAVVSIDYRLAP
                     EHCAPAGIDDCEDAVRWLAEGAGDLGGDPGRLAVAGDSAGATLAALVAIRLRDDRGPR
                     LAHQALIYPATDLSQSFPSIHEHAHAPVLDKAALDAFRIHYLGDSGIPPEDPRVSPYF
                     TADLAGLPPALIQTADLDPLRDEGLAYADRLTAAGVPVRVTNYLGTVHGFMSFPGATV
                     VGPQARLELVTELRRHLLGD"
     gene            1313..2317
                     /locus_tag="PKJ61_12360"
     CDS             1313..2317
                     /locus_tag="PKJ61_12360"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020390351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HOC14652.1"
                     /translation="MADVVVFSGNAHPALAQRICSSLGVRLSGVNLSRFSNDCLQAQL
                     LANCRQRDVYIIQPLVPPTQEHLMELLLMIDAARGSSAASITAVMPHYAYARSDKKDA
                     SRISIGGRLVADLLKTAGVTRVLTMALHAPQVHGFFRVPVDHLTAIGVLADHYRAMNL
                     DNHIVVSPDLGNAKTATLFARLLNLPVAAGSKQRMADDKVVIDAIVGDVAGKRAIVLD
                     DEIATGGSIVELLDRLADAGCIDASVACTHGLFTGKAVERLRGHPFIREVVTTDTVPP
                     PQGWPELEVRSVAELFAEAISRIHQGKSVSKLFRGVDPTHAPPQPSLPFDDHPTLPTL
                     "
BASE COUNT          359 a          931 c          757 g          409 t
ORIGIN      
        1 tcgatcccca tgacccgccg caccgtcata gcgccaccct atgtccgctc cggcccacgg
       61 gagcgtgagc cgcacatcga aggcatcgac agcgtcggcc gcgccggttc cggtgcggca
      121 cactggccgc atgcctcgta tgccgttgcg gacccgggcg ctcgccgcgc tcttgcgccg
      181 tgggatcttc ccgtcgatcg cgacgatgga cctggctgcc atccagcgct cacgggcccg
      241 tcggctgccc tcccgggcac ccgtggcgtg ggtggtcggc cgggtctcgc gtgacctgca
      301 ggtcagcatg gagtggttcc ccacccggga cggcagccgc cggcaggcgc gcgtctaccg
      361 tccggcagga cagccgccgc agggcggctg gccggtcgtc tgctacttcc acggtggcgg
      421 ctttgtcctg ggcagcatca cgcagagcga cccgatctgt tcgcacctgg ccagtgccat
      481 tcccgcggca gtcgtgtcga tcgactacag gcttgcgcct gaacattgcg ctcctgccgg
      541 gatcgacgac tgtgaggatg cggttcgctg gctggcggag ggtgcgggcg atctcggcgg
      601 cgatccgggt cggctagctg tggcgggaga cagcgccggc gcaacgctgg ccgccctcgt
      661 cgcaatccgg ctgcgcgacg accggggacc acggttggcc caccaagccc tcatctaccc
      721 ggcgaccgac ctcagccagt ccttcccgtc gatccacgag catgctcacg caccggtcct
      781 ggacaaggcc gccctcgacg ccttccgcat ccactacctc ggcgatagcg ggatcccgcc
      841 cgaggacccc cgcgtgtctc cctacttcac tgccgatctc gccggcctgc cgccggcgct
      901 catccagacc gccgatctcg acccgctgcg cgacgaagga ctggcctatg ccgaccgcct
      961 caccgccgcg ggtgtgcccg tccgtgtcac caattacctg ggcaccgtgc atggcttcat
     1021 gagcttcccc ggggccaccg tggtcggccc gcaggcccgc cttgaactgg tcaccgaact
     1081 ccgacggcat ctgctgggtg actaagactg cacgatgacc acggctgctc gatgagcgag
     1141 acaccgcccg ggcctgctga ggagggcctg gctcccgtcc gagcgcagtc ggcaaccgca
     1201 ccggcatacc ggtgtgatgt gtgccgctgt cgcctgcgcg cccttcccgg gtcccggcag
     1261 ccctggtcta gggtttgacc tggatcccgc agcccgcatc gaaagggacc tcgtggcgga
     1321 cgtcgtcgtc ttctccggaa acgcccatcc cgctctggct caacgcatct gctcgagtct
     1381 cggcgtccgc ctctcgggcg tcaacctcag ccggttcagc aacgactgcc tgcaggcgca
     1441 gttgctggcc aactgccgcc aacgcgacgt ctacatcatc cagccgctgg tgccccccac
     1501 tcaggagcac ctcatggaat tgctgctcat gatcgacgct gcgcgcgggt catcggccgc
     1561 atcgatcacg gcggtcatgc cgcactacgc gtatgcgcgg tcggacaaga aggacgcgtc
     1621 gcggatctcc atcggtggcc ggctggtggc cgacctgctc aagaccgcag gcgtgacccg
     1681 cgtgctcacc atggccttgc atgcgcccca ggtgcacggc ttcttccggg tgccggtcga
     1741 ccacctcacc gccatcggag tgctcgccga tcactaccgg gcgatgaacc tcgacaacca
     1801 catcgtcgtc tctcccgacc tgggcaacgc caagaccgcc acgctcttcg ctcgtctgct
     1861 caacctcccg gtcgcagcgg gaagcaagca gcgaatggcc gacgacaagg tggtcatcga
     1921 cgccatcgtc ggcgatgtcg ccggcaagcg cgcgatcgtc ctggacgacg agatcgccac
     1981 cggcgggtcc atcgtcgagt tgctcgatcg gctcgccgac gccggctgca tcgacgcctc
     2041 ggtcgcctgt acccatggcc tgttcaccgg caaggccgtc gaacggctgc gcggtcatcc
     2101 cttcatccgc gaagtcgtca ccaccgacac cgtcccgccg ccgcagggct ggcccgagct
     2161 agaggtgcgc tcggtggcag aactcttcgc cgaggcgatc tcgcgcatcc accaaggtaa
     2221 gtcggtgtcc aagctcttcc ggggtgtcga ccccacgcat gccccacctc agccgtcgct
     2281 tccgttcgat gaccacccga ccctgccgac gctatgacct cactcccgaa tccccccgca
     2341 gccgaccgca accctatcgg tatgccgtgg gccctgctgc tcgacctcgt gctcgtcgtc
     2401 gtcttcgccg cgatcggccg ggccagccat gccgagcaga acgccgtcgt aggcgc
//
LOCUS       DAOPRQ010000157         2382 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_35342_length_2382_cov_11.091534, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000157 DAOPRQ010000000
VERSION     DAOPRQ010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35342_length_2382_cov_11.091534"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(71..>2382)
                     /locus_tag="PKJ61_12365"
     CDS             complement(71..>2382)
                     /locus_tag="PKJ61_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOC14653.1"
                     /translation="HPLVCTAFNADFDGDQMAVHLPLSAEAQAEARILMLSTNNILKP
                     ADGRPVTLPTQDMVIGHYFLTTERSDLVGEGRAFSSVAEAYMAFDRHELAIGAPCRIR
                     LTGVVPPEGLELRPDGSIIMDTTLGRALFNEALPTDYPFVNVEVGKKKLGQIVNDLAE
                     RYPKVEVAVTLDKLKSLGFKWATRSGVTVSIGDVQTPPNKPEILAAYEARANKITKQY
                     ERGKVTEEERHQELIQLWTDATAELTAAMEANFDKTNPIVMMVHSGARGNMTQMRQIA
                     AMRGLVANPKGEIIARPIKSNFREGLSVLEYFISTHGGRKGQADTALRTADSGYLTRR
                     LVDVSQDVIIREEDCQTDRGLTKAIAAEGKNGKLAAARNVETAVYSRTLATDVVSAAG
                     KVLVKAGVDLGDVNIKKLLKNGITEVKVRSVLTCEAATGTCAMCYGRSLATGKLVDVG
                     EAVGIVAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVVELFEARQPKGKAPIAEA
                     SGRVRIEEGERTRKLVIVRDDGEADLEYPLPRRVRLEWEDATGVRHSVGDGDHVAAGR
                     QLYAGTPDPQDVLRVSGLRKVQEHLVEEVQAVYRTQGAPIHDKHIEIIIRQMLRRITV
                     IESGDTSMLPGELVDRKSFEAQNRQMVTEGRTPAQGRPVLMGITKASLATESWLSAAS
                     FQETTKVLTDAAINAKSDSLLGLKENVILGKLIPAGTGLERYRNIRVEPTAEARAAAY
                     DLSYDPYDYDLSVGGSSVGLDEYDTGELR"
BASE COUNT          358 a          813 c          789 g          422 t
ORIGIN      
        1 gagccgtgaa cagcagaatc ccgccgagac cagcggtctc ggcgggattc ggggcttgcc
       61 tagcggcgga tcagcgcagt tcgccggtat cgtactcgtc cagcccgacc gacgaaccgc
      121 cgaccgaaag gtcgtagtcg tacgggtcgt aggagaggtc gtaagcagcc gcccgcgcct
      181 ccgcggtggg ctccacccgg atgttgcggt accgctccag gccggtgccg gcagggatca
      241 gcttgcccag gatgacgttc tccttgaggc ccagcagcga gtccgacttc gcgttgatcg
      301 ctgcgtcggt gagcaccttg gtggtctcct ggaacgaagc cgcggacagc cacgactcgg
      361 ttgcaaggga cgccttggtg atgcccatca gcaccggacg gccctgggcc ggggtacggc
      421 cttcggtcac catctggcgg ttctgcgcct cgaacgactt ccggtcgacc agctcgcccg
      481 gcagcatcga cgtgtcgccg gactcgatca ccgtgatccg acgaagcatc tgccggatga
      541 tgatctcgat gtgcttgtcg tggatcggcg caccctgcgt ccggtacacg gcctgcacct
      601 cttcgaccag gtgttcttgc accttgcgca gacccgacac gcgaagcacg tcctgcgggt
      661 cgggcgtacc ggcgtagagc tgccgtccgg ccgcaacgtg atcgccgtcg ccgacagagt
      721 gccggacgcc ggtcgcgtcc tcccactcca ggcggacgcg ccgcggcagc gggtactcca
      781 ggtcggcctc gccgtcgtcg cggacgatga ccagcttgcg ggtacgctcg ccctcctcga
      841 tccgcacccg gccggacgcc tcagcgatcg gcgccttgcc cttgggctgt cgggcctcga
      901 agagctcgac cacacgcggc agaccctggg tgatgtcgtc accggccaca ccaccggtgt
      961 ggaaggtacg catcgtcagc tgggtgccgg gctcgccgat cgactgggcc gcgacgatac
     1021 cgacagcctc gcccacgtcc accagcttgc cggtggccag cgagcggccg tagcacatgg
     1081 cgcaggtgcc ggtggccgcc tcacaggtga ggacggagcg caccttgacc tcggtgatgc
     1141 cgttcttgag cagcttcttg atgttcacgt cgcccagatc gacgccggcc ttgaccagca
     1201 cctttccggc ggcgctcacc acgtcggtgg ccagcgtgcg ggagtagacc gcggtctcca
     1261 cgttgcgggc tgcggccagc ttgccgttct tgccctcggc cgcgatcgcc ttggtgagcc
     1321 cccgatcggt ctggcagtcc tcctcgcgga tgatcacgtc ctgcgacacg tccaccagac
     1381 gccgggtcag gtacccggag tcggccgtgc gcagtgcggt gtccgcctgg cccttccggc
     1441 caccgtgcgt ggagatgaag tactccagca cgctcaggcc ctcacggaag ttggacttga
     1501 tcggccgggc gatgatctcg cccttcgggt tggccaccag gcctcgcatg gcggcgatct
     1561 ggcgcatctg ggtcatgttc cctcgggcgc cggagtgcac catcatgacg atcgggttcg
     1621 tcttgtcgaa gttggcctcc atcgcggcgg tcagctcggc ggtagcgtcc gtccagagct
     1681 gaatcagctc ctggtgccgc tcctcctcgg tgaccttgcc acgctcatac tgcttggtga
     1741 tcttgttggc acgcgcctcg taggccgcca agatctccgg cttgttcggc ggagtctgca
     1801 cgtccccgat cgaaacggtc acacccgagc gggtggccca cttgaaaccg agcgacttca
     1861 gcttgtccag cgtgaccgcg acctcgacct tggggtaccg ctcggcgagg tcgttgacga
     1921 tctggcccag cttcttcttg cccacttcga cgttgacgaa cgggtagtcc gtcggcagcg
     1981 cctcgttgaa cagcgcccgg cccagggtgg tgtccatgat gatggaaccg tcaggacgca
     2041 gttcgaggcc ctccggcggc accacaccgg tgagccggat gcggcaaggc gcgccgatcg
     2101 ccagctcgtg gcgatcgaaa gccatgtacg cctcggcgac ggacgagaac gcccgaccct
     2161 caccgaccag atccgaccgc tcggtggtca ggaagtagtg gccgataacc atgtcctgcg
     2221 taggcagggt gaccggacgg ccgtccgcgg gcttcaggat gttgttggtg gacagcatca
     2281 ggatgcgcgc ctcggcctgc gcctccgcgc tcagcggcag gtgcacggcc atctggtcgc
     2341 cgtcgaagtc ggcgttgaag gcggtgcaga ccagcgggtg ca
//
LOCUS       DAOPRQ010000158         2364 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_35655_length_2364_cov_17.207882, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000158 DAOPRQ010000000
VERSION     DAOPRQ010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35655_length_2364_cov_17.207882"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="PKJ61_12370"
     CDS             complement(<1..226)
                     /locus_tag="PKJ61_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016669346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I site-specific deoxyribonuclease, HsdR"
                     /protein_id="HOC14654.1"
                     /translation="MTIPPSGSHSYAPIAVSSESTVVAEFNPDAAASEGYQSEAQLEA
                     SFIEQLVKQGYERLAITSSAELIANLRRQLE"
     gene            491..1537
                     /locus_tag="PKJ61_12375"
     CDS             491..1537
                     /locus_tag="PKJ61_12375"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOC14655.1"
                     /translation="MAEARASLASLEATANRLPNPRMFRTASLRLEAQATAALEGTYE
                     PLERVLAADDDDTSDPDLLEVLNYLLVARQAFTWSEQGRGWGVRDLAELQALLVRGTA
                     SEREYSGQVRPIQVVIGRRESAAPGELPIKAARYVPPPPGPDLEARLRDVSDWMQADH
                     SEAIDPIVAAAMGHYAFEAAHPFHDGNGRIGRLLIVMQLLASRTLTEPTLTVSPWFEA
                     RRTEYYDALFGVSARSDWSTWVSFFARGLGESADATRARMLALVSVQADLKERLQSTP
                     IRTANARILIDYAVGHATFTVGQAAAALGMSHAGAKKLIDSMVTHGLLAPWGKRNYNR
                     QFHAPEVMRTLLQG"
     gene            complement(1607..>2364)
                     /locus_tag="PKJ61_12380"
     CDS             complement(1607..>2364)
                     /locus_tag="PKJ61_12380"
                     /inference="COORDINATES: protein motif:HMM:NF025625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HOC14656.1"
                     /translation="EAQRNGAYLLLGDDDEAVEGLNCYIGRTENFVRRFDDHQRKKEF
                     WDRVVLITSKDDAFNEGHWGYLEARLVEFAKQAARCTLRNDNTPQGRRLSEAQASDME
                     AFLAQLQIVLPVLGVNVIRTRTTRTQPTPASDSPTFHLMNAKLGVNAQAQVNGDEFTM
                     LSGSVVVAAWTTVGRAESTRRSYAAYRAQHEKLVADGSIVVEGGRGRLTRDIPFSSPS
                     TAAAVALGRSSNGRIEWTWDGGTYAAWENRGLA"
BASE COUNT          390 a          768 c          780 g          426 t
ORIGIN      
        1 cctcaagctg gcggcgcagg ttggcgatca gctcggccga cgaggtgatc gcgagccgct
       61 cgtagccctg cttgaccagt tgctcgatga aggacgcctc cagttgcgcc tcgctctggt
      121 agccctcgga cgccgcggcg tccgggttga actcggcgac gacggtcgac tccgagctga
      181 ccgcgatcgg agcgtaggag tgcgacccac taggcgggat agtcacgctc cgaacctagc
      241 gttctcggcg cttcggtcga tcgcggccac gcgactcgaa ggacgtcagg aaaagttaca
      301 gccatgaagt tttccaaagc cgcgctaaag ttgcattgtg gatatagaac tgttccagaa
      361 ggaagccttc ggtgagctcg tacccgtcaa gggctcgacg ccggcaggtg ttccgtggca
      421 gcacttcgcg ttccttcctc atccgctcgg cgatgacagt ccaccactcg accccacctg
      481 ctatcgccgt gtggcagagg ctcgcgcttc gctggcgtcc ctcgaagcga ccgctaaccg
      541 gcttcccaat ccgaggatgt ttcgaacggc gtccctcagg ctggaagcac aggcgactgc
      601 agctttggaa ggcacctacg aaccgctcga gcgcgtgctc gctgccgatg acgacgacac
      661 gagcgatccc gacctgctcg aagttttgaa ctacctcctg gtggcgcgcc aagccttcac
      721 ttggtcggag caggggcgtg gctggggtgt gcgcgacctg gcggagctcc aggcgctgct
      781 ggtgagggga actgcttccg agcgggagta ctccggacaa gtccgaccca tccaggttgt
      841 gatcggtcgt cgcgagtcgg ccgctcccgg tgaactaccg atcaaagccg ctcgctacgt
      901 gccgccgcca cccgggcctg acctggaggc gagactccgc gatgtttcgg actggatgca
      961 ggccgaccac tcggaagcga tcgatcccat cgtggctgcg gcaatggggc actacgcctt
     1021 cgaggctgcc cacccgttcc acgacggcaa cggacggatc ggtcgactgt tgatcgtcat
     1081 gcaactgctg gcgtcgcgga ctctcaccga gcccacgctc acggtctcgc catggttcga
     1141 agcccgacgt accgagtact acgacgcact cttcggtgtg tcggctcgat cggactggtc
     1201 gacatgggtc tcgttcttcg cgcgcgggct gggtgagtcc gcggatgcca cgcgagcgcg
     1261 catgctggcg ctcgtttcgg tgcaggcgga tctcaaggag cgcctccaat ccaccccgat
     1321 ccggacggcc aacgcgcgga tcctcatcga ctacgccgtg ggtcatgcca cattcaccgt
     1381 gggacaggcc gcggcggcgc tggggatgtc ccacgcggga gcgaagaagc tcatcgattc
     1441 catggtcacg cacgggttgc tggcaccttg gggaaaacgc aattacaacc ggcagttcca
     1501 tgcgccggag gtgatgcgga ccttgctgca gggctgaggc caccacccgc gtacgctcgg
     1561 ttcgactttg ttcaccctcg cggcgaccca aacgaactaa acaaagtcag gcgaggccgc
     1621 ggttctccca ggccgcgtag gtgccgccgt cccaggtcca ctcgatgcgg ccgttgctgg
     1681 aacggccgag cgccacggcg gcagcggttg agggggagct gaacgggatg tcacgggtca
     1741 gtcggccgcg tccgccctcg acgacgatgg acccgtccgc gacgagcttt tcgtgctggg
     1801 ctcggtaggc cgcgtaggag cggcgcgtgc tctcggctct gccgacggtc gtccaggcgg
     1861 ccacgacgac cgagccggac agcatcgtga actcatcccc attgacctga gcctgcgcgt
     1921 tgacgccgag cttggcgttc atcaggtgaa acgtcggcga gtcggacgct ggtgtcggct
     1981 gggtgcgcgt ggtccgggtg cggatgacgt tgacgcccag caccggcaga acgatctgca
     2041 gttgggccag gaacgcctcc atgtcggacg cctgtgcctc cgacagcctg cgaccctggg
     2101 gcgtgttgtc gttgcggagc gtgcagcggg cggcctgctt ggcgaactca acgagccgcg
     2161 cttcgaggta accccagtgc ccctcgttga aggcgtcgtc cttggacgtg atgaggacga
     2221 cccgatccca gaactccttc tttcgctggt ggtcgtcgaa ccggcggacg aagttctcgg
     2281 tgcgcccgat gtagcagttc agcccctcca cggcctcgtc gtcgtcgccg agcagcaggt
     2341 aggcgccgtt gcgctgcgct tccg
//
LOCUS       DAOPRQ010000159         2168 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_39818_length_2168_cov_15.512068, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000159 DAOPRQ010000000
VERSION     DAOPRQ010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39818_length_2168_cov_15.512068"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(28..627)
                     /locus_tag="PKJ61_12385"
     CDS             complement(28..627)
                     /locus_tag="PKJ61_12385"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOC14657.1"
                     /translation="MTIRALLMDADGVVQYPRSGWLERFARLGGPGFTREAFRREMST
                     LTGQVDLRDELAEIIADGGHDATPEDFIEIWCDIEVDAYMLRLVDRVRAAGVVTALAT
                     NQQSYRGTHMLTNLPHADHFDHQFHSFQMGLAKPDPAFFRYIIEALGIAADEAVFVDD
                     MEINVRGAREAGLQGVYFSAADTYGHLRSRLRELGVPGI"
     gene            complement(642..>2168)
                     /gene="ilvD"
                     /locus_tag="PKJ61_12390"
     CDS             complement(642..>2168)
                     /gene="ilvD"
                     /locus_tag="PKJ61_12390"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020387343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HOC14658.1"
                     /translation="CADALICISNCDKITPGMLLAALRLNIPTVFVSGGPMEAGRILG
                     DRVVTGAPDEGGSARLDLIDAMVKAVDDNISDDEIAAIEENACPTCGSCSGMFTANSM
                     NCLTEALGLSLPGNGSTLATAAARKELFLEAGRLAVRLANRYYGEDDESVLPRSVATK
                     EAFANAMRLDIAMGGSTNTVLHILAAAREAGVDFTMNDIDALSRVTPCLSKVAPNTTK
                     YYMEDVHRAGGMPAILGELRRGGLLDENVHAVHSDTLAEWLDTWDIRGGAASAPANDL
                     WYAAPGGVRTTQAFSSTNRWADLDTDAADGCIRSVEHAYTADGGLAVLKGNLAVDGCI
                     VKTAGVPEDQWTFRGPAVVTESQEAAVEAILSKRVQPGDVVVVRYEGPKGGPGMQEML
                     YPTSYLKGLGLGPKCALITDGRFSGGSSGLSIGHVSPEAASGGTIGLVEDGDIIEISI
                     PDRSINLLVDDATLAERRARQDAIGWNPQGRVREVSTALKVYAALALSADKGAARSID
                     "
BASE COUNT          341 a          723 c          744 g          360 t
ORIGIN      
        1 ctagggcgtg tcgttgcgca acacgcccta gattccgggg acgccgagtt ccctcagtcg
       61 agagcgcaga tggccgtagg tgtcggcggc ggagaagtac accccctgca ggccggcttc
      121 ccgggccccg cggacgttga tctccatgtc atcgacgaac acagcctcgt ccgccgcaat
      181 cccgagagcc tcgatgatgt agcggaagaa cgccggatcg ggcttggcca accccatctg
      241 gaacgagtgg aactggtggt cgaagtggtc ggcgtggggc aggttggtca gcatgtgcgt
      301 gccgcgatag ctctgctggt tggtggccag tgcggtcacc acgccggcag cccggacgcg
      361 gtcgaccagc cgcagcatgt aggcgtccac ctcgatgtcg caccagattt cgatgaagtc
      421 ctcaggcgtg gcgtcgtggc cgccgtcggc gatgatctcg gcgagttcgt cgcgcaggtc
      481 cacctggccg gtgagggtgg acatctcgcg ccggaacgcc tccctcgtga acccagggcc
      541 accgagtcgc gcgaaccgct ccagccagcc cgaacgcggg tactgcacga cgccgtcggc
      601 gtccatcagc agggcacgga tggtcacgtc tctccccggc ttcagtcgat cgagcgggcc
      661 gcacccttgt cggcgctgag cgccagcgcg gcatacacct tcagcgccgt cgacacctcg
      721 cggacgcgtc cctgggggtt ccacccgatc gcgtcctggc gggcacggcg ttcggcgagc
      781 gtggcgtcgt ccaccagcag gttgatgctg cggtcaggga tcgagatctc gatgatgtcg
      841 ccgtcctcga ccaggccgat ggtgccgccg gacgcggctt ccggcgacac gtggccgatc
      901 gacagccccg acgagccgcc ggagaagcgt ccgtcggtga tcagggcgca tttcgggccg
      961 aggccgagcc ccttcaaata ggacgtcggg tagagcatct cctgcatacc cggcccgccc
     1021 ttcgggccct cgtagcgcac caccaccaca tcgccgggtt ggacgcgctt gctcaggatc
     1081 gcctcgaccg cggcctcctg cgactcggtg accacggccg ggccgcggaa cgtccactgg
     1141 tcttccggga cgcccgcagt cttcacgatg cagccgtcga cagcgaggtt gcccttcagc
     1201 acggccaggc cgccgtccgc ggtgtaggcg tgctcgactg agcggatgca gccgtcggcc
     1261 gcgtcggtgt cgaggtctgc ccagcggttg gtcgaactga acgcctgtgt ggtgcggacg
     1321 ccgccgggtg cggcatacca gaggtcgttg gccggggcgg aagctgcgcc gccacggatg
     1381 tcccaggtat cgagccactc ggcgagcgtg tcggaatgaa ccgcgtgcac gttctcgtcc
     1441 agcagaccgc cgcggcgcaa ttcgccgagg atcgcgggca taccgccagc gcgatgcacg
     1501 tcttccatgt agtacttcgt agtattcggg gctactttgc tgaggcacgg agtgacccgc
     1561 gaaagcgcgt cgatatcgtt catggtgaag tccacgccgg cctcgcgagc cgcagccaga
     1621 atgtgcagca ccgtgttggt agagccgccc atcgcgatat ccagacgcat ggcgttggca
     1681 aatgcctcct tggtcgcgac cgatcgcggc agcaccgact cgtcgtcctc gccgtagtac
     1741 cggttggcca gccgcaccgc caggcgtccg gcttcgagga acagttcctt gcgggcggct
     1801 gcggtggcca gggtcgaacc gttgccgggc agcgaaaggc cgagcgcctc ggtgaggcag
     1861 ttcatcgagt tagcggtgaa catgcccgag caggagccgc aggtggggca ggcgttctcc
     1921 tcgatcgcgg cgatctcgtc gtcactgatg ttgtcgtcca cggccttgac catggcgtcg
     1981 atgaggtcga gccgggccga gccaccctcg tccggggcac cggtcaccac tcgatcgccg
     2041 aggatgcgcc cggcctccat cgggccgccg ctgacgaaga ccgtcgggat gttcagccga
     2101 agcgcggcca gcagcatgcc gggggtgatc ttgtcgcaat tggagatgca gatcagggcg
     2161 tccgcgca
//
LOCUS       DAOPRQ010000160         2160 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_40011_length_2160_cov_9.635154, whole genome shotgun sequence.
ACCESSION   DAOPRQ010000160 DAOPRQ010000000
VERSION     DAOPRQ010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40011_length_2160_cov_9.635154"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1390)
                     /locus_tag="PKJ61_12395"
     CDS             complement(<1..1390)
                     /locus_tag="PKJ61_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOC14659.1"
                     /translation="MSNRPLHDRLAALVSGAVAGAVAITGLAAYVITTLAVYDQLDRE
                     LIDIAAVTSKWIRGDGDTDALGGLNSDALSTANVTMLRVRADGKTTAPPGAPNQLVPT
                     ARELAVARTQTGSSARSGVDSAGESYRIVAVPYTDGTSKYALVLGRPLAPTDEILRIL
                     AFSLLTFGLLAVLMAGAIGWVIARSGLQPIQRLTDAVIHVTKTDQFEPIETGGMDELT
                     DLTTSFNTMMKTLESSRERQRRLIADAGHELRTPLTSLRTNVELLIADDRQGMLPEGA
                     RGEILRDISAQLGEFTQLIGDLVHLSREDRVEAHPEPIDLRDVVTGALVRAKRRGPGL
                     NFDVELDPLFLVGEPDSLERAITNLLDNAVKFSPAGGTITVRLKGDRLRISDEGSGIA
                     DADLPHVFDRFYRSETSRNTPGSGLGLSIVAQTIKAHGGWVKAGRSESGGAEFTIRLP
                     GSSTAEEEAEITD"
     gene            complement(1417..2106)
                     /locus_tag="PKJ61_12400"
     CDS             complement(1417..2106)
                     /locus_tag="PKJ61_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOC14660.1"
                     /translation="MHILVVDDDQAVRDSLARSLHYSGWEVTTANDGIEALARLSALR
                     PDAVIMDVMMPRLDGLETTRSLRASGNDVPILVLTARDAVGDRVDGLDAGADDYMAKP
                     FSFDELQARLRALTRRVRPATDEPGAEVLSFADLTLDLQTREVTRAARKISLTRTEFA
                     LLQTFLENPRRVLERGWLLNEVWGFDFPTTANSLEVYIGYLRRKTEGEGEARLIHTVR
                     GIGYVLRETPP"
BASE COUNT          331 a          740 c          741 g          348 t
ORIGIN      
        1 cgtcggtgat ctcggcttcc tcctcggcgg tgctggatcc gggcaggcgg atggtgaact
       61 ccgcgccgcc ggactccgag cgtccggcct tcacccagcc accgtgcgcc ttgatggtct
      121 gcgcgacgat cgatagcccg agccccgaac cgggggtgtt ccgcgaagtc tccgagcgat
      181 agaaccggtc gaagacgtgc ggtaggtcgg cgtccgcgat gcctgaaccc tcatccgaga
      241 tccgtagccg gtcgcccttg agccgcacgg tgatcgttcc gccggccggg gagaacttca
      301 cggcgttgtc cagcaggttg gtgatcgccc gctccaagct gtcgggttcg ccgaccagga
      361 agagcgggtc gagttcgaca tcgaagttga gcccgggccc gcggcgcttg gctcgcacca
      421 gcgccccggt caccacgtcg cgcaggtcga tcggttccgg atgggcctcc acccggtcct
      481 cacgcgacag gtgcaccagg tcgccgatca gctgggtgaa ctcgccaagc tgagcggaga
      541 tgtcacgcag gatctcaccc cgcgcaccct cggggagcat gccctgacgg tcgtccgcga
      601 tcagcaactc cacgttggtt cgcagcgagg tgagcggagt ccgcagctcg tggcccgcgt
      661 ccgcgatcaa tcgccgctgg cgctcgcggg acgattcgag ggtcttcatc atcgtgttga
      721 acgaggtggt cagatcggtc aactcgtcca tgccgccggt ctcaatcggc tcgaactggt
      781 cggtcttggt gacgtggatc actgcgtcgg tcagccgctg gatcggctgc agccccgagc
      841 gcgcgatcac ccagccgatg gcgccggcca tcagcacggc cagcaggccg aacgtcagca
      901 gcgagaaggc caggatgcgc agaatctcgt ccgtcggcgc cagcggacgc ccgagcacca
      961 gcgcgtactt cgaggtgccg tcggtgtacg gcacggcaac gatccggtac gactcacccg
     1021 ccgagtccac cccgctgcgc gcggacgagc cggtctgcgt ccgggcgacc gccaactccc
     1081 gggcggtggg caccagttgg ttcggcgccc cgggaggtgc ggtggtcttg ccgtccgctc
     1141 gaacccgaag catggtgacg tttgcggtgg acagggcgtc gctgttcagt ccgccgaggg
     1201 catcagtgtc gccgtcgccc cggatccatt tgctggtcac cgcggcgatg tcgatcagtt
     1261 cccggtcgag ttggtcgtac accgcaagcg tggtgattac gtaggcggcc aggccggtga
     1321 tcgccaccgc gccagccaca gcccccgaca ccagcgctgc cagccggtcg tgcaggggcc
     1381 gattgctcac cgagaggacg gatcgcagcc agcggatcac ggcggcgtct cgcgcagcac
     1441 gtagccgatg ccgcgcacgg tgtggatcag tcgcgcttca ccctcgccct cggtcttgcg
     1501 gcgcaggtag ccgatgtaaa cctcgagtga gttggcggtg gtggggaagt cgaagcccca
     1561 gacctcgttc aacagccagc cacgttccag cacccggcgc gggttctcca ggaacgtctg
     1621 cagcagggcg aattcggtcc tggtcaggct gatcttgcgc gccgcgcggg tgacctcgcg
     1681 ggtctgcagg tcgagggtca ggtcggcaaa gctcagcacc tcggcgccag gctcgtcggt
     1741 ggccggacgc acccgccggg tcagcgcccg cagccgtgcc tgcaactcgt cgaaggagaa
     1801 cggcttggcc atgtagtcat cggcgccggc gtccagcccg tcgacccggt caccgaccgc
     1861 gtcccgcgcg gtgagtacca ggatcggcac gtcgttgccg gacgcgcgca gcgaccgggt
     1921 cgtctccagc ccgtcgaggc gcggcatcat tacgtccatg atcaccgcgt ccggacgcag
     1981 tgcggacagc cgggcgagcg cttcgatccc gtcgttggcc gttgtcacct cccaaccgga
     2041 gtagtgcagc gaccgggcca gcgagtcgcg gaccgcctga tcgtcgtcga caacgaggat
     2101 gtgcatggca ccagcctgcc acgtgcggct cagccacgtc gcgccggacg cgtgtggtgg
//
LOCUS       DAOPRQ010000161         2154 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_40158_length_2154_cov_8.652215, whole genome shotgun sequence.
ACCESSION   DAOPRQ010000161 DAOPRQ010000000
VERSION     DAOPRQ010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40158_length_2154_cov_8.652215"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            146..766
                     /locus_tag="PKJ61_12405"
     CDS             146..766
                     /locus_tag="PKJ61_12405"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOC14661.1"
                     /translation="MQQLTANTYWLTDAVGAHCYWLDAGDRVAIVDPGMSPGLNRVAR
                     ELRRAGRSPYEVTDILLTHADADHAQAAAELQRRTGARCWLGAADAAVLTGAAQPGSA
                     FRRFTVRFGRPELPRHLNLLDGDAEPWPGLASLHTPGHTPGHFSFVTGRVLFGGDTAL
                     MSGTGLEPMHPFLMTDVEQGLVDLARLSRLDVDWYCFGHSDPVRRR"
     gene            complement(884..1423)
                     /locus_tag="PKJ61_12410"
     CDS             complement(884..1423)
                     /locus_tag="PKJ61_12410"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOC14662.1"
                     /translation="MAQRYPEITDRLAKFIEAQQLFFVATAARDGHVNVSPKGLDSLR
                     ITSPNQVVWVNGTGSGNETAAHLLDSPRMTIMFCSFVREPLILRLYGTARAVHPRDDE
                     WAGLIGLFPPIRGARNVFVLDVDLVQTSCGYGVPKFDFVEQRDLMDAWAIKKGPDGLA
                     DYQDKHNLVSMDGLPTGLR"
     gene            1617..>2154
                     /locus_tag="PKJ61_12415"
     CDS             1617..>2154
                     /locus_tag="PKJ61_12415"
                     /inference="COORDINATES: protein motif:HMM:NF015213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HOC14663.1"
                     /translation="MFLASLLIGLREGLEASLIVGILIAYVRKRGRDDVVPRIGWGVA
                     IAIVGSLALGAILTFGAYGLSFQGQEIIGGLMSLVAVGMVTWMVFWMMRTGHRLKAEL
                     EGEAAEALALGSGWAIAWIAFISVGREGIETTLMLWGWAGQPEALLGALVGIGVAAGI
                     GYLLYRGMVRINLGTFFAW"
BASE COUNT          333 a          743 c          715 g          363 t
ORIGIN      
        1 ccggccgatc gagcccgtcg agatcaccac ccggctgatc gagcttgtcg agatcacccg
       61 gacgggtctc gacaagctcg accacccgag acgcctggct tgacgaggcc cccagcgccg
      121 cccacgatgg gcagcaggag acgacatgca gcaactcacc gcgaacacct actggctcac
      181 cgacgcggtc ggcgcgcact gctactggct ggacgccggt gatcgggtcg caatcgtcga
      241 ccccggcatg agcccgggcc tcaaccgggt ggcgcgagag ctgcgcagag ccggccggtc
      301 gccctacgag gtgaccgaca tcctgctgac ccacgccgac gcggaccacg cccaggccgc
      361 cgcggagttg caacggcgga cgggcgcccg ctgctggctc ggcgcggcgg acgcggcggt
      421 gctcaccggc gccgcgcagc ccggcagcgc ctttcgacgg ttcaccgtcc ggttcggacg
      481 acccgagttg ccccggcacc tcaacctgct cgacggcgac gccgagccgt ggcctgggct
      541 ggcctccctg cacacccccg gccacacgcc gggacacttc agcttcgtca ccggacgcgt
      601 tctcttcggt ggcgacaccg cgctcatgtc cgggactggc ctcgagccga tgcacccgtt
      661 cctgatgacc gacgtggagc agggcctggt cgacctcgcc cggctcagcc gcctagacgt
      721 ggattggtac tgcttcggcc acagcgatcc ggtgcgccgc cgctgaaccc ttgtcggcag
      781 atcgagcttg tcgagatcac caccacctcg gctgatcgag cttgtcgaga tcaccatcct
      841 ggtctcgaca agctcgacca cccgaccggc tggggaacgg gaatcagcgc aggccggtgg
      901 gaagtccgtc catgctgacc aggttgtgct tgtcctggta gtcggccagg ccgtcggggc
      961 ccttcttgat tgcccaggcg tccatcaggt cacgctgctc gacgaagtcg aacttgggaa
     1021 cgccgtagcc gcaggaggtt tgcaccaggt ccacgtccag cacgaagacg ttccgagcgc
     1081 cccggatcgg cgggaacagc ccgatcaggc cggcccactc atcgtcccgg ggatggacgg
     1141 cgcgggccgt cccgtacagc cgcaggatca gcggctcgcg cacgaacgag cagaacatga
     1201 tggtcatccg ggggctgtcg agcaggtgcg cggccgtctc gttgccgctg ccggtgccgt
     1261 tgacccacac cacctggttc ggcgaggtga tgcgcagact gtccagaccc ttcggggaga
     1321 cgttcacgtg tccgtcccgg gcggcggtgg cgacgaagaa cagctgttgg gcttcgatga
     1381 acttcgccag ccgatcggtg atctcggggt agcgctgggc catggcatca gcatgcctcc
     1441 tggccgcctc gatcgcgacc gccggccatc gcagccggcg cccggcgagc gcgtttccgg
     1501 ggggtttgcc cgcaacctgg gccgacgtta gcgcggctgt ggtgattttg ctaaggtctg
     1561 cgttgcttaa tcttcttagg taaccctttc ctaatcaatc gagagagaag aggccggtgt
     1621 tcctcgcctc gctactcatc gggctccgcg aaggccttga agcttcgctg atcgttggca
     1681 tcctgatcgc ctacgtgcgc aagcgtggac gcgacgacgt ggtcccccgc atcggctggg
     1741 gcgtcgcgat cgccatcgtc ggttcgctcg ccctcggcgc gatcctcacc ttcggcgcct
     1801 acggcctcag cttccaggga caagagatca tcggcggcct gatgtcgctt gtggcggtcg
     1861 gcatggtcac ctggatggtc ttctggatga tgcgcaccgg ccaccgcctc aaagccgagc
     1921 tggaaggcga agcagccgaa gcactcgccc tgggcagcgg ctgggcgatc gcctggatcg
     1981 cgttcatctc ggtcggacgc gagggcatcg agaccaccct catgctctgg gggtgggccg
     2041 gccagcctga agccctgctt ggtgcgctgg tcggcatcgg agtcgccgcc ggcatcgggt
     2101 acctgctgta ccgcggcatg gtgcgaatca acctgggcac gttcttcgcg tgga
//
LOCUS       DAOPRQ010000162         2099 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_41519_length_2099_cov_21.112035, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000162 DAOPRQ010000000
VERSION     DAOPRQ010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41519_length_2099_cov_21.112035"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..884
                     /locus_tag="PKJ61_12420"
     CDS             <1..884
                     /locus_tag="PKJ61_12420"
                     /inference="COORDINATES: protein motif:HMM:NF016432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HOC14664.1"
                     /translation="IDRMFRQHDVAPADVVSPLYRQLTQEWAELHITPSTPATVRRWG
                     RTEKALAGFTLPGQIVDAIDAAGHDQTNELLLALIRLFQRGHQLAGRVVLQALLPKLA
                     KTATHAGGMCTSSSDTWAEDRRHITLAEFWDVMADYPVERRTSSVASNLALDTLHRVT
                     GARKPPTDLPVDPHDFRDEETRDGARRTWLAGLSTTDPDPVEEITPDSDLLEVIAWGM
                     RKATISQDEAQLLVASYLPERTQGFGFATVAEQLGLSQAAVRQRCSRASRKLTEAVRA
                     ELHSPAPLTTVDQAVCA"
     gene            complement(974..1723)
                     /locus_tag="PKJ61_12425"
     CDS             complement(974..1723)
                     /locus_tag="PKJ61_12425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14665.1"
                     /translation="MPGGGGGGGGGGGGGGGGGGGGSNIVVLVVDGQRCTYAGLKNPQ
                     PSKDDPVWEGHTDGAIYACEVAPQVRFGSIIAGYQIPFWAATAPALPPPDPRVLAQQA
                     MDSMNLKAINIGIVPEDRPGSIGIIGLPSWMWVDAPVENTMGPITRSASARGYTVTAV
                     AKVTKIVWNMGDGRTVTCTGPGTPYADSYGKQSSPDCGHTYTRQGRYTVSATSYWTVE
                     WAGIGQTGTIPIDFTTRTNITMGESQVINQR"
     gene            complement(1861..>2099)
                     /locus_tag="PKJ61_12430"
     CDS             complement(1861..>2099)
                     /locus_tag="PKJ61_12430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14666.1"
                     /translation="RVDLKLDLKKSPPDVPTVQLDVCIDVSKLNVVDKDGKSAIPADR
                     KPRQLWRVGVSNYEYPKADAWRVSYTDSRGDKTC"
BASE COUNT          347 a          760 c          654 g          338 t
ORIGIN      
        1 agatcgaccg gatgttccga cagcacgacg tcgcccccgc cgacgtcgtc tcacccctgt
       61 accgccagct gacccaggag tgggccgagc tgcacatcac ccccagcacc ccggcaacgg
      121 tgcgccgctg gggtcgcacc gagaaggcac tggccgggtt cacactgccc ggacagatcg
      181 tcgacgcgat cgacgccgcc ggccacgacc agaccaacga gctcctgctg gccctcatcc
      241 gcctcttcca gcgcggccac cagctcgccg gccgagttgt cctccaggcg ctgctgccca
      301 agctcgccaa gaccgccacc catgccggcg ggatgtgtac ctcgagcagc gacacctggg
      361 cggaggaccg ccggcacatc acgctggccg agttctggga cgtgatggcc gactacccgg
      421 tcgagcggcg caccagctcc gtcgcctcga acctcgccct ggacaccctc caccgcgtca
      481 ccggagccag gaagccaccc acggacctgc cggtcgatcc tcacgacttc cgcgacgagg
      541 agacccgcga cggcgcccgg cgcacctggc tcgccgggct cagcaccacc gaccctgacc
      601 cggtcgagga gatcaccccc gactcggacc tgctcgaggt catcgcctgg ggcatgcgca
      661 aggccaccat cagccaggac gaggcacagc tgcttgtcgc ctcctacctg ccggagcgaa
      721 cccaaggctt cggattcgcg accgttgccg agcagctcgg gctcagccag gccgccgtcc
      781 ggcagcgctg ctcgcgcgcg tcccgcaagc tcaccgaggc agtccgcgcc gagctccact
      841 cccccgcacc tctcaccacc gtcgaccagg cggtctgcgc ctgacctcac tcccctgctg
      901 gcaagcaaga gagccggccc tcacccaaga tctgggtgag ggccggctct ttggcggggc
      961 cgagcgggca ggactacctc tgattgatca cctgggactc gcccatcgtg atgttcgtcc
     1021 tggtggtgaa gtcgatcggg atcgtcccgg tctgaccgat gcccgcccac tccacggtcc
     1081 agtacgacgt agccgacacc gtgtagcggc cctgacgggt gtaggtgtga ccgcaatccg
     1141 gggagctctg cttgccatag ctgtcggcat acggcgtgcc cgggccggtg caagtcaccg
     1201 tcctgccgtc acccatgttc cagacgatct tggtcacctt cgccacggcg gtcaccgtgt
     1261 aaccacgagc cgaggcagag cgggtgatgg gccccatggt gttctcgact ggcgcgtcga
     1321 cccacatcca gctcggcagc ccgatgatcc cgatgcttcc gggacggtct tccggcacga
     1381 tcccgatgtt gatcgccttg agattcatcg agtccatagc ctgctgagca agcacccgcg
     1441 ggtctggcgg tgggagcgcg ggagcggtcg cagcccagaa cgggatctgg tacccggcga
     1501 tgatgctgcc aaaccgaacc tgaggagcaa cctcgcacgc atagattgcg ccgtcggtgt
     1561 ggccttccca caccgggtca tccttggatg gctgggggtt cttcagaccg gcgtaggtgc
     1621 atctctggcc gtcgacgacg aggacgacga tgttgcttcc gcctccacca ccgccaccgc
     1681 cgccgccgcc accgccgcca ccgccgccgc cgccgccggg cacttcgatc cagatgacgc
     1741 agatgccggt tcggggatcg gtcttttgac agacggggtc ctcagcattc gcggaagtcg
     1801 ctgtgccagc gagcatgaac gccgcggcca gcagggtgag gagccaagtg gctactcgct
     1861 tcaacatgtc ttatcccccc tgctgtcggt gtaggaaaca cgccaggcgt cagccttcgg
     1921 gtactcgtag ttggaaactc cgacccgcca cagttggcga ggcttgcggt ccgccgggat
     1981 ggcggacttg ccatccttgt ccacgacgtt gagcttgctg acgtcgatgc acacgtcgag
     2041 ctggacggtt ggaacgtccg gcggggactt cttgaggtcg agcttgaggt cgacacggg
//
LOCUS       DAOPRQ010000163         2041 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_43086_length_2041_cov_13.202920, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000163 DAOPRQ010000000
VERSION     DAOPRQ010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43086_length_2041_cov_13.202920"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="PKJ61_12435"
     CDS             complement(<1..265)
                     /locus_tag="PKJ61_12435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020575216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HOC14667.1"
                     /translation="MTNATPATPEPSESARKEQEQAAKDALARVNQREADAAWRSIAY
                     LLTGPAVYGGLGWLLDRWLDTSWIAVLGICGGMALSLYLIWFRY"
     gene            complement(341..832)
                     /locus_tag="PKJ61_12440"
     CDS             complement(341..832)
                     /locus_tag="PKJ61_12440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14668.1"
                     /translation="MTPTHEPQRTTSGQSAGSQSDAFAELLRGSIVPTGIVAVVCVAV
                     ALFSGAKAGWSAAFGAFLVIAFFTVSLVVMKRTAHLAPTTVMAIVMVTYTAKVLALGV
                     AMFLLRDASWVNGYAVGVTITICTLVWLFFEMRAYKRLRIFAFTPSSTDSAGDTPQSG
                     RQA"
     gene            complement(933..>2041)
                     /locus_tag="PKJ61_12445"
     CDS             complement(933..>2041)
                     /locus_tag="PKJ61_12445"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HOC14669.1"
                     /translation="VREYLLIAVIAMAVTYAATPLVRALAVVFGAVTPLRDRDVHRVP
                     IPRLGGVAIFLGFAAATLIARELPYLKGVYASGQMTGVLLGAAIVVAVGALDDIRELD
                     WWAKLAGQVLAAGVMAYKGVVLLSVPFFGTSTVLPGPVLVAVTVFVVLATTNAVNFID
                     GLDGLAAGIVAIASFAFFVYSYVLSHTYDPPNVFSTATFVTAATLGCCLGFLPHNFNP
                     ARLFMGDSGALLLGLLLAAATISLTGSVDPSSVSGDQLTAALLPILAPLAVLSLPFLD
                     MLLAVLRRTRAGQRPWQPDAKHLHHRMLAIGHGHRGAVLILYLWAAVLALGTVSLAFF
                     RGWLTVVAILVATVVAAVCTAYLPRWFPPKAPKP"
BASE COUNT          393 a          673 c          707 g          268 t
ORIGIN      
        1 cgtaccggaa ccagatcagg tagagcgaca gagccatccc cccgcagatg cccagaacgg
       61 cgatccacga ggtgtccagc caccggtcca gcaaccagcc cagcccgccg tagacggccg
      121 gtccggtcag cagataggcg atcgagcgcc atgcggcgtc cgcttcgcgc tggttgaccc
      181 gcgccagcgc atccttggcg gcctgttcct gctctttccg ggccgattcg gagggttccg
      241 gggtggctgg ggtggcgttc gtcacgacga gtccttccga ccgtcagggt ccgaatcggg
      301 ccgaggttcg cctggtccgg accggcagcg gtgaccagaa tcacgcctgc cgtccactct
      361 gtggcgtgtc gccggcgctg tctgtcgagc tgggggtgaa ggcgaagatc cgcagccgct
      421 tgtacgcccg catctcgaag aagagccaca ccagggtgca gatcgtgatg gtgaccccga
      481 cggcataccc gttgacccac gacgcgtcgc gcagcaggaa catcgccact ccgagcgcaa
      541 gtaccttggc cgtataggtg accatgacga tcgccatgac ggtggtcggc gcgaggtgcg
      601 ccgtgcgctt catgacgacc aggctcaccg tgaagaacgc aatcacgagg aaggcgccga
      661 aagccgccga ccagccggcc ttggctcccg agaagagcgc gaccgccacg cagaccaccg
      721 cgacgatgcc ggtcgggacg atcgaccctc ggagcaactc ggcgaaggcg tcgctctgac
      781 tgccggcgga ctggccagag gtcgtccgct ggggctcatg cgtcggggtc atcggctact
      841 ttcgatgtag tcggccagga gggctgagag ccgctcacgt gggctgcggc agcgcttgtg
      901 aaagctatca caagcaccga gaaggccact actcacggct tcggtgcctt gggcgggaac
      961 caccggggca ggtatgccgt gcacacggca gcaaccaccg tggcgaccag gatcgcgacg
     1021 acagtgagcc acccgcggaa gaaggccaac gacaccgtcc cgagggccag gacggcagcc
     1081 cacagataga gaatgaggac agcgccgcgg tgcccgtgac cgatggccag catgcgatgg
     1141 tgcaggtgct tggcgtccgg ttgccaggga cgctggccgg cccgggtgcg tcgcagcacc
     1201 gccagcagca tgtcgaggaa gggcagtgac aacaccgcca ggggcgcgag gatcggcagc
     1261 aacgcggccg tgagctggtc gccgctcacc gagctcggat cgacactgcc ggtcagcgag
     1321 atggtggctg ccgcgagcag tagccccagc aggagcgcgc ccgagtcgcc catgaagagc
     1381 cgggcgggat tgaagttgtg cggcaggaag cccaggcagc acccgagggt cgccgcggtg
     1441 acgaaggtag cggtcgagaa gacgttgggc gggtcgtagg tgtgcgagag cacgtacgag
     1501 tagacgaaga aggcgaagct cgcgatggcg acgatcccgg cggccagtcc gtcgagcccg
     1561 tcgatgaagt tgaccgcatt ggtcgtggcc agcacgacga agaccgtcac cgccacgagc
     1621 accggaccgg gcagcaccgt gctcgtgccg aagaagggca ccgacagcag aaccacgccc
     1681 ttgtaggcca tgacgccggc cgcgagcact tgcccggcca acttggccca ccagtcgagt
     1741 tccctgatgt cgtcaagcgc ccccaccgcc acgacgatcg ccgcaccgag cagcactccg
     1801 gtcatctgac cgctggcgta gacgcccttg agatagggca actcccgcgc gatgagggtg
     1861 gccgcggcaa agccgaggaa gatcgccact cccccgagcc gtgggatcgg gacccggtgc
     1921 acgtcccgat cccgcaaggg ggtcaccgca ccgaacacga ccgccagggc ccggacgagc
     1981 ggggtcgctg cataggtcac cgccatggcg atgacggcga tcagcagata ctcgcgcacg
     2041 c
//
LOCUS       DAOPRQ010000164         1835 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_49866_length_1835_cov_11.669663, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000164 DAOPRQ010000000
VERSION     DAOPRQ010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49866_length_1835_cov_11.669663"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /gene="rplI"
                     /locus_tag="PKJ61_12450"
     CDS             complement(<1..434)
                     /gene="rplI"
                     /locus_tag="PKJ61_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013866126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOC14670.1"
                     /translation="MKLILTTPVNHLGIPGDIVEVKDGYGRNYLLPRNFAIKWTRGAE
                     KQIEGIKRARDAREIRGVEHASAVREQLEALTVNLPARAGDAGKLFGAVTPADVALAV
                     KKAGGPAIDKRSVEIAKPIKTVGNHTVGIKLTAGVVAHISVAV"
     gene            complement(451..687)
                     /gene="rpsR"
                     /locus_tag="PKJ61_12455"
     CDS             complement(451..687)
                     /gene="rpsR"
                     /locus_tag="PKJ61_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015071887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOC14671.1"
                     /translation="MASQRKPVKSKKVAPAKSIRIGHIDYKDTATLRKFISERGKIRA
                     RRVTGLSVQEQRKVAIAVKNARELALLPYASTSR"
     gene            complement(854..1360)
                     /locus_tag="PKJ61_12460"
     CDS             complement(854..1360)
                     /locus_tag="PKJ61_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002516468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOC14672.1"
                     /translation="MAGETIITVVGNLTADPELRFTPSGAPVANFTVASTPRTFDRQS
                     GEWKDGDAMFLNCAVWRQAAENVAESLTKGMRVIVQGRLGSRSYETREGEKRTVFELQ
                     VDEVGPALKYATAKVTRSSGGGGGGNYSGGGSSGGGSSWGNNSGGQSDRSGGADPWAS
                     AQSEEPPF"
     gene            complement(1443..1733)
                     /gene="rpsF"
                     /locus_tag="PKJ61_12465"
     CDS             complement(1443..1733)
                     /gene="rpsF"
                     /locus_tag="PKJ61_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007434229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOC14673.1"
                     /translation="MRKYEVVVIIDPDVDDRQVNGLLEKPLAGLTKAGGTVDNIDVWG
                     RRRLAYDIRKKSEGIYAVINLTAEPAVVKELDRQFTLNEQIMRTKVIRPDLH"
BASE COUNT          291 a          592 c          599 g          353 t
ORIGIN      
        1 acggccacgc tgatgtgcgc gacgacacca gcggtcagct tgatgccgac cgtgtggttg
       61 ccgacggtct tgatcggctt ggcgatctcg accgagcgct tgtcgatggc cgggccacca
      121 gccttcttca ccgcgagggc gacatcggcc ggggtgaccg caccgaacag cttgccggcg
      181 tcacccgcgc gagccggcag gttcacggtg agtgcctcca gctgctcgcg gacggcactg
      241 gcgtgctcga cgccacggat ctcgcgggcg tcgcgagccc gcttgatgcc ctcgatctgc
      301 ttctcggcgc cgcgcgtcca cttgatcgcg aagttgcggg gcagcaggta gttgcggccg
      361 tagccgtcct tgacctcgac aatgtcaccc gggatgccca ggtggttgac cggggtggtg
      421 agaatcaact tcatttctgt cctcccctga tcagcgtgag gtcgaggcgt agggcagcag
      481 cgccagctcg cgggcgttct taaccgcgat ggcaaccttc cgctgctcct gcaccgagag
      541 tccggtaacc cgacgagcgc ggatcttgcc ccgctcggag atgaacttcc gcagcgtggc
      601 ggtgtccttg tagtcgatgt ggccgatgcg aatggacttc gccggcgcca ccttcttcga
      661 tttcacaggc ttgcgctgtg aggccattgt ggtgctctcc ttcatgaaag cccggctctc
      721 gccggaatga cgtgtttcga tggacgtcgt tcgctccgcc tgtagccggc acccgacacg
      781 cgtcccctcg tcaggctcag ggatcgtgcg aacgctggct accgtgcgtc agctcacggc
      841 gtccggggaa cgatcagaac gggggctctt cgctctgggc cgaggcccag gggtcagcgc
      901 cgccgctgcg atcggattgg ccgccggagt tgttgcccca ggaggagccg ccaccggacg
      961 agccaccgcc ggagtagttg ccaccgccgc caccgcccga agagcgggtg accttggcgg
     1021 tcgcgtactt cagcgcggga ccgacctcat cgacctggag ctcgaagacg gtgcgcttct
     1081 caccttcccg agtctcgtag ctgcgcgacc ccagccggcc ctgaacgatg acccgcatgc
     1141 ccttggtgag ggactcggca acgttctcgg cagcctggcg ccagacggcg cagttcagga
     1201 acatggcgtc cccgtccttc cactcgccgc tttggcgatc gaaggtgcgg ggcgtggagg
     1261 cgaccgtgaa gttcgccacc ggtgccccgg acggagtgaa gcgcagttcg ggatcggcgg
     1321 tcagattgcc gaccaccgtg atgatggttt cgccagccat ggttgatgtc tttcagtcgg
     1381 acttagcttt gacccctaca gagtgtcagt agccactgac agttcgatag ggcggaaagc
     1441 gatcagtgca gatcggggcg gatcaccttg gtccgcatga tctgctcgtt gagggtgaac
     1501 tgacgatcca gttccttcac cacggccggc tcagcggtca ggttgatgac ggcgtagatg
     1561 ccttcggact tcttccggat gtcataggcc agccgacggc ggccccacac atcgatgttg
     1621 tccacggtgc caccggcttt ggtgaggccg gcgagcggct tctccagcag gccgttcacc
     1681 tggcgatcgt cgacgtcggg atcaatgatc accacgactt cgtacttgcg catacgcgaa
     1741 ctccacctcc tctggtctag agcggccacg ggcgcttccc gtggcaggag ggcgtatcgg
     1801 ttcccggaat gggaaccaag gtcgaactct accgg
//
LOCUS       DAOPRQ010000165         1812 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_50718_length_1812_cov_11.245874, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000165 DAOPRQ010000000
VERSION     DAOPRQ010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50718_length_1812_cov_11.245874"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="PKJ61_12470"
     CDS             complement(<1..359)
                     /locus_tag="PKJ61_12470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020392655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HOC14674.1"
                     /translation="MAKILQFDEEARRSLERGVDILANTVKVTLGPKGRYVVLDKKWG
                     APTITNDGVTVAKEVELSDPFENLGAQLAKEVATKTNDVAGDGTTTATVLAQALVHEG
                     LRAVASGANPIALKRGI"
     gene            complement(371..667)
                     /gene="groES"
                     /locus_tag="PKJ61_12475"
     CDS             complement(371..667)
                     /gene="groES"
                     /locus_tag="PKJ61_12475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004808944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOC14675.1"
                     /translation="MAVTIKPLEDRILVQPLEAEQTTASGLVIPDTAKEKPQEGKVLA
                     TGPGRIDDNGNRIPLDVAEGDVVIFSKYGGTEVKYNGQEYLLLNARDILAVVTK"
     gene            857..>1812
                     /locus_tag="PKJ61_12480"
     CDS             857..>1812
                     /locus_tag="PKJ61_12480"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOC14676.1"
                     /translation="MNWPDSVIWWHVYPLGFVGAERSASHEVQHRLPQLVNWLDYLID
                     LGCNGLLLAPVFASASHGYDTLDHYRIDPRLGDEHDFRALVDECHARGIRVCLDGVFN
                     HLDRDHPIVQRALAAGPDSEDGRWIRWSGEHPYCFEGHSRLVELDLSQPVVAKYVAQV
                     MNHWLDRGADAWRLDAAYGQGAEVWAPILAEVKAAHPDAWVFAEVIHGDYAEFVRAAG
                     VDSVTQYELWHAIWDSLNDGNFFSLDWTLQRHREFCKSFRPQTFIGNHDVTRIATKLS
                     NPSHVSLATALLMLLPGIPSIYAGDEQGFTGLKEENHYGDDAV"
BASE COUNT          274 a          565 c          631 g          342 t
ORIGIN      
        1 tcgataccgc gcttcagcgc gatcgggttg gcgccggacg cgaccgcgcg caggccctcg
       61 tgcacgagcg cctgggccag gacggtggcg gtggtggtgc catcgccggc cacgtcattg
      121 gtcttggtgg cgacctcctt ggccagctgg gcgccgaggt tctcgaacgg atcggagagc
      181 tccacctcct tggcgacggt cacgccgtcg ttggtgatgg tcggggcgcc ccacttcttg
      241 tcgaggacga cgtagcgccc cttgggcccg agggtcacct tcacggtgtt ggcgaggatg
      301 tcgacaccac gctcgagcga gcggcgggcc tcctcgtcaa actgaaggat ctttgccatg
      361 tgtgagttcc ttacttggtg actacggcga ggatgtcgcg ggcgttgagc agcaggtact
      421 cctggccgtt gtacttgacc tcggtgccgc cgtacttgct gaagatgacg acgtcgccct
      481 cggccacgtc gagcgggatg cggttgccgt tgtcgtcgat gcggccgggg ccggtggcga
      541 gcaccttgcc ctcctgcggc ttctccttgg cggtatcggg gatgaccagg ccggaggcgg
      601 tggtctgctc ggcttccagc ggctggacga ggatgcgatc ctcgagcggc ttgatcgtga
      661 ctgccacgtc aataaccttt cgttcttgca ggacgcgaac gccctgatga tcaggtgttt
      721 cccggcctcg gcgtcgtcgc gggtgccacc gaaggctgat tggcaccctc aaggtgagag
      781 tgctaagtcg aaatgtatcg cgggcttagc actcgatcaa gccgagtgcc agccggtgat
      841 ggctaagctg gtgggtatga attggcccga cagcgtgatc tggtggcatg tgtacccgct
      901 gggcttcgtc ggcgcggagc gcagcgcgtc ccacgaggtg cagcaccgcc tgccgcaact
      961 cgtgaactgg ctcgactatc tgatcgatct cggctgcaac gggctgctgc tcgcgccggt
     1021 cttcgcgtcc gccagccacg gttacgacac gctcgatcac taccgcatcg atccccggct
     1081 cggggacgag cacgacttcc gggccttggt ggacgagtgc cacgcccgcg gtatccgggt
     1141 gtgcctggac ggggtcttca accacctcga ccgggatcac ccgatcgtgc agcgggcgtt
     1201 ggcagcgggc ccggacagcg aggacggccg ctggatccgc tggtcgggcg agcatccgta
     1261 ctgcttcgag gggcattccc ggctggtcga gctggatctc agccagccgg tcgtggcgaa
     1321 gtacgtggca caggtgatga accactggct cgaccgtggt gcggacgcct ggcgcctgga
     1381 cgccgcctac ggccagggcg ccgaggtctg ggcgccgatc ctggccgagg tgaaggccgc
     1441 gcacccggac gcgtgggtgt tcgccgaagt gatccatggc gactacgccg agttcgtccg
     1501 cgcggccggt gtggattcgg taacgcagta cgagctgtgg cacgcgatct gggactcgct
     1561 gaacgatggg aatttcttct ccctggattg gacactgcag cggcatcgtg agttctgcaa
     1621 gagcttccgt ccgcagacgt tcatcggcaa ccacgacgtc acccggatcg cgaccaagct
     1681 gagcaatccg tcgcatgtct cgctggccac ggccctgctg atgctgctgc cggggatccc
     1741 gtccatctac gccggggacg agcagggctt caccgggctg aaggaggaga accactacgg
     1801 cgacgacgcc gt
//
LOCUS       DAOPRQ010000166         1735 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_53804_length_1735_cov_18.867857, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000166 DAOPRQ010000000
VERSION     DAOPRQ010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53804_length_1735_cov_18.867857"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1159)
                     /locus_tag="PKJ61_12485"
     CDS             complement(<1..1159)
                     /locus_tag="PKJ61_12485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14677.1"
                     /translation="MSSDATPANPMTDEQRIARAAEVATSEIAMKLCAAAERRTGRPR
                     LIPLSGVLAAYIFHATGEPHTMTTSGVCRSIKKLKPRQRTALGIPKRMQIRYKRMHSG
                     WRAIKRVVEHGVLVEHDHELEVDPDTGEVLPCPEGCPFEQVGLDELTTMLIQASLPPE
                     FEPPSAVAIDGTDVESHTRPHKKRIDAEGRAFWSADRDARWGHRTATDRRPAEHFLGY
                     EAHLATYAPRVGSEPIPQVVAGLALRPGIRDRARACLNIVDTLPPFDDLLVDRGYTTA
                     KGSQLAGPLRDRGITVTMDLHKTQRVVRPGPEAGTVWVDGTLYTSALPETHRTLEAPT
                     AGMSAAEKARRREAFDAREPYRFVPHAKRDTKRGTQRFRGPAVDGHPFKVRC"
     gene            complement(1198..1611)
                     /locus_tag="PKJ61_12490"
     CDS             complement(1198..1611)
                     /locus_tag="PKJ61_12490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14678.1"
                     /translation="MWKANVGDLELVHGFLCVRVTESRHPRVVPITAPNDEILASFLT
                     EPADAPILAYRPRKWDRSPFHEMLRKADVPPGCPTITPARLRATWMVDHLTHGMSMGG
                     LTTLAGFTSWRSYQPLQQYIPQPDEPTLFAQAVRR"
BASE COUNT          233 a          524 c          669 g          309 t
ORIGIN      
        1 ggcagcggac cttgaaggga tgcccgtcga ctgctgggcc gcggaagcgc tgcgtgcccc
       61 gcttcgtgtc gcgcttggcg tgtgggacga accggtaagg ctcgcgggcg tcgaacgctt
      121 cacggcgtcg tgccttctcg gcggcgctca ttccagcggt gggtgcttcg agcgtgcggt
      181 gggtctcggg taacgcgctg gtgtagaggg ttccgtcgac ccagacggtc ccggcctcag
      241 ggccgggccg gacgacccgc tgggtcttgt gcaggtccat cgtcaccgtg atgcctcggt
      301 ctcggagcgg gccggcgagc tggctgccct tggctgtggt gtagccacgg tcgacgagga
      361 ggtcatcgaa tggtggcagc gtgtcgacga tgttcaggca ggctcgggcg cgatcacgga
      421 ttcctggtcg cagcgcgagg cccgccacta cctgagggat gggttcggac ccgacgcggg
      481 gggcgtaggt ggcgaggtgg gcttcgtagc cgaggaagtg ttcggcgggg cggcggtcgg
      541 tggcggtgcg gtggccccat cgggcgtcgc ggtcggctga ccagaacgca cgaccttcgg
      601 catcaatgcg cttcttgtgc ggtcgggtgt ggctctcgac gtccgtgccg tcgatcgcca
      661 ccgccgaagg tggctcgaac tccggcggca ggctggcctg gatgagcatc gtcgtgagct
      721 cgtccagccc gacctgctcg aacgggcaac cctcagggca gggcaggact tcgccggtgt
      781 ccggatcgac ctcgagctca tggtcgtgct cgaccaggac accgtgctcg acgacgcgct
      841 tgatggcccg ccagccggag tgcatccgct tgtacctgat ctgcatgcgc ttcgggatcc
      901 cgagcgccgt gcgttgcctc ggcttgagct tcttgatgga tcggcataca ccggaggtgg
      961 tcatcgtgtg cggctcgccg gtggcgtgga agatgtaggc ggcgaggact ccggacagtg
     1021 ggatgaggcg aggccgtccg gtccgccgtt ccgccgccgc gcagagcttc atggcgatct
     1081 cgctggtggc gacctcggcg gcccgcgcga tgcgctgttc gtccgtcatc gggttggccg
     1141 gcgttgcgtc cgagctcacc cgcgaaccaa cggcgctcgg aggccgtcgc gatcggctca
     1201 ccggcggacc gcctgggcga acagtgtggg ctcgtccggt tgcgggatgt actgctgcag
     1261 gggctggtag gaccgccacg aggtgaagcc ggccagcgtg gtgagcccgc ccatggacat
     1321 gccgtgggtg aggtggtcga ccatccaggt ggcccgcagc cgggccgggg tgatcgtcgg
     1381 acagccgggc gggacgtcgg ccttgcgcag catctcgtgg aagggtgagc ggtcccactt
     1441 cctcggccgg tacgccagga tcggcgcgtc ggccggttcg gtgaggaagc tggcgaggat
     1501 ctcgtcgttg ggtgcggtga tcggaaccac ccggggatgc ctgctctcgg tgacgcggac
     1561 gcagaggaag ccatggacca actcgaggtc gccgacgttg gccttccaca tctcgtgcgg
     1621 atgcaaccca gcgcccagtc caagggctag gagcgtgggt cagtaacacg ccagtcgggt
     1681 tccgctgctg aagtgggcgc ttgggggaga atctggtgtg cctgatgatg acgcg
//
LOCUS       DAOPRQ010000167         1732 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_53924_length_1732_cov_10.638640, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000167 DAOPRQ010000000
VERSION     DAOPRQ010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53924_length_1732_cov_10.638640"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            105..692
                     /locus_tag="PKJ61_12495"
     CDS             105..692
                     /locus_tag="PKJ61_12495"
                     /inference="COORDINATES: protein motif:HMM:NF020120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase N-terminal
                     domain-containing protein"
                     /protein_id="HOC14679.1"
                     /translation="MCIGSNSIVIEAGRDRFAARGGDTWGWSVATWHREPMALLRLEM
                     TLADDSHTHIQTDNSWHAAAGPVVEKFFQGERWIVDGGAPEWRPATVVAAPAGELRRA
                     THPAPERMASIAPVTASPQGAGRTVYDFGDVITGRLTCQAVGGPGAAVEVVSGEQRAA
                     DGSVICDNVLVAGPGQRDSLHFAAAHEQFNWEARF"
     gene            717..>1732
                     /locus_tag="PKJ61_12500"
     CDS             717..>1732
                     /locus_tag="PKJ61_12500"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14680.1"
                     /translation="MTVTGEASVSKVRAVPIYTPLEEVGAFQCSNPVVEWVDAALPAT
                     FRNNLHGVPTDTPIYEKNGWTADAHLATAALLHRFDLRASFGKWMDDHVGAQTATGAI
                     PQIIPTPGWSTESGPAWSSSAVLIPWYLYREYGEIATLERYAPMARRYADDLLSHLRD
                     DLWTGRTWGDWLAPGYMVPPEGMTPIGTLMTITALQHTAQILEELEEDASSYRDAAAR
                     IGRRYHSQYFDPHSEHYAVEGVGYRQSLNILPLAFGAVPPEWIASVRKIVTDDIELRT
                     AGHLVCGALGARHLVDVLTDAGRDDLALTILTNPTRPGWGQWFSDGERTLLETWDADA
                     RSRN"
BASE COUNT          314 a          593 c          552 g          273 t
ORIGIN      
        1 gacgggcgaa acagcgccgc tgttgacgcg agtgaatagg gttctgcgca cctagcagga
       61 accctgcgca ccgagcagga accctgcgga cagtagccca tctactgtgc attggatcca
      121 actcgatcgt gatcgaagcc ggacgagatc gcttcgccgc ccgtgggggt gatacctggg
      181 gctggagcgt cgccacatgg caccgggagc cgatggcgtt gttgcggctc gagatgactc
      241 tggctgacga ctcccacacg cacatccaga cggacaacag ctggcacgcc gccgccggac
      301 cggtcgtaga gaagttcttc cagggtgaac ggtggatcgt cgatggcggg gcacccgaat
      361 ggcggcccgc gaccgtcgtc gccgctcccg cgggcgagct ccgccgcgcg actcacccgg
      421 cgccggaacg gatggcatcg atagcgcccg tcacggcctc cccacagggc gcggggcgca
      481 ctgtctacga cttcggcgac gtcatcacgg gtcgactcac gtgtcaggcg gtcggcgggc
      541 caggcgcagc cgtggaggtg gtcagcggcg agcaacgggc cgccgatggg agcgtgatct
      601 gtgacaacgt cctggtcgcg ggcccgggcc agagagactc actccacttc gctgcagccc
      661 acgagcagtt caactgggag gcgcgcttct gataccgcgg cttccgatgg atccaagtga
      721 ctgtcaccgg tgaagcgtcc gtgagcaagg tcagggcggt cccgatctac accccactcg
      781 aagaagtcgg cgcatttcag tgcagcaacc cagtcgtcga gtgggtcgac gcggcgttgc
      841 ccgccacgtt ccggaacaac ctccatggcg tccccaccga cactcccatc tacgagaaga
      901 acgggtggac agccgacgcc cacctcgcca ccgcagccct gctacaccgc ttcgatctgc
      961 gagcgtcgtt cggcaagtgg atggacgacc atgtgggcgc ccagactgcc acgggcgcca
     1021 tcccacagat catcccgacg cccggatggt caacggaatc cggcccggct tggtcctcgt
     1081 cggccgtgct tatcccgtgg tacctgtacc gcgaatacgg cgagatcgcg acccttgagc
     1141 gttacgcgcc catggctcgc cggtacgccg atgacttgct cagccaccta cgcgacgacc
     1201 tctggactgg acgcacctgg ggagactggc tcgccccggg gtacatggtc ccgccggagg
     1261 gcatgacgcc gatcggcacc ctcatgacca tcaccgcgct tcaacacacc gcacagatcc
     1321 tcgaggagtt ggaggaggac gctagctctt atcgcgacgc cgccgcacgg atcggcaggc
     1381 gctaccacag ccagtacttc gacccccatt cggagcacta cgccgtcgag ggcgtcggct
     1441 accgacagag cctgaacatc ttgccgttgg cgtttggtgc cgtcccgccc gagtggatcg
     1501 cgtcggttcg caagatcgtc accgacgaca tcgagctcag gaccgctggc cacctggtct
     1561 gtggtgccct cggcgcccgg catcttgtcg acgttctcac cgacgccgga agagatgacc
     1621 tagcgctgac gattctcacc aacccgaccc gaccgggctg ggggcaatgg ttcagcgacg
     1681 gagagcgaac cctgctggag acttgggacg ccgatgcccg ctctcgcaac ca
//
LOCUS       DAOPRQ010000168         1655 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_57361_length_1655_cov_9.982500, whole genome shotgun sequence.
ACCESSION   DAOPRQ010000168 DAOPRQ010000000
VERSION     DAOPRQ010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57361_length_1655_cov_9.982500"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(96..275)
                     /locus_tag="PKJ61_12505"
                     /pseudo
     CDS             complement(96..275)
                     /locus_tag="PKJ61_12505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611786.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(307..1593)
                     /locus_tag="PKJ61_12510"
     CDS             complement(307..1593)
                     /locus_tag="PKJ61_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HOC14681.1"
                     /translation="MLTVVHDAAQPNEDDAALGASLLDEIVRDGARKMLAAALQAEVA
                     AYIEAHADQVDEHGHRLVVRNGYHAERRVTTAAGAVTVRQPRVNDRRTDEGTGQRMRF
                     SSTILPAWARKSPRVTEVLPLLYLHGLSSSDFAPALEQFLGTSHGLSAATITRLTSQW
                     QNDARAFAARSLADVDYVYLWVDGIHLKVRLEQDKVCLLVMIGVRADGTKELVALADG
                     FRESSESWADLLRDCKRRGMRAPVLAVGDGALGFWKALRDVFPDTREQRCRWHKIGNV
                     LAALPKSAHPGAKKALAEIYNAEDRDHAVAAAKAFAAAYGAKWPKAAAKITDDLDVLL
                     AFYAYPAEHWIHLRTTNPIESTFATVRLRQRVTKGPGSRAAGVAMAFKLIESAQRRWR
                     AVNAPHLVALVRVGAKFENGKLVERPDESGGDAHAA"
BASE COUNT          248 a          564 c          562 g          281 t
ORIGIN      
        1 tcggcaacct cagcgacacc ccgacgatct cccaagccgg gtgagcttcc gggccctcgg
       61 ccgggtctgc tgcacgatcg ggtgacaggt tggggtcacg cagcctgagt ggctggcgtg
      121 gtcatgatgg tctcgaactc gatgggggtc aatcggccca gagcggcctg ccgacgacgg
      181 cggtggtagg tcctctcgat ccacgtgacg atcgcgattc gcagctcctc gcgagtggtc
      241 caggagcggc ggttgaggac gttcttctgg agcaagagga atagtcaaga cctgtggatc
      301 ggcgtgtcac gcggcgtgag catcgcctcc tgattcgtcg ggtcgttcga cgagcttgcc
      361 gttctcgaac ttggcgccga cacggaccag ggcgaccagg tggggtgcgt tcacggcccg
      421 ccaccggcgt tgggccgact cgatgagctt gaacgccatc gccaccccgg ccgctctgga
      481 gccggggccc ttggtcactc gctgccggag cctgaccgtc gcgaaggttg actcgatcgg
      541 attcgtggtg cgcagatgga tccagtgctc ggccgggtag gcgtagaacg ccaggagcac
      601 atcgaggtcg tcggtgatct tggcggccgc cttcggccac ttcgcgccgt acgccgcggc
      661 gaacgccttg gcggcggcga cggcgtggtc acggtcctca gcgttgtaga tctcggccag
      721 cgccttcttc gctcccgggt gcgccgactt cggcagcgcg gccagcacgt tgccgatctt
      781 gtgccaccgg caccgctgtt cgcgggtgtc gggaaagacg tcacgcagtg ccttccagaa
      841 cccgagcgcg ccgtcgccga ctgcgaggac cggggcgcgc atgccgcggc gtttgcagtc
      901 ccgcagcagg tcggcccacg actcggatga ttcgcggaac ccgtcggcga gcgcgaccag
      961 ttccttggtg ccgtcggcgc ggaccccgat catcaccagc aggcagacct tgtcctgctc
     1021 gagccggact ttcaggtgga tcccgtcgac ccacaggtag acgtagtcga cgtcggccag
     1081 cgaccgggcc gcgaacgcgc gcgcgtcgtt ctgccactgg ctggtcaacc tggtgatcgt
     1141 cgccgccgac aggccatgac tggtgccgag gaactgctcc aacgccgggg cgaagtcgga
     1201 actggacagc ccgtgcaagt acagcagcgg cagcacctcc gtgacccgcg gcgacttgcg
     1261 cgcccacgcc ggcaggatcg tcgaggagaa ccgcatccgc tgcccagtgc cctcgtccgt
     1321 gcgcctgtca ttcacgcggg gctggcgcac cgtcaccgcg cccgcggccg tggtgacccg
     1381 ccgttcggcg tggtagccgt tgcggaccac caaccggtga ccgtgctcgt cgacctggtc
     1441 cgcatgcgcc tcgatgtagg cggcgacttc ggcctgcaac gccgccgcga gcatcttgcg
     1501 ggcaccatcg cgaacgatct catccagcaa cgacgcccca agcgccgcgt cgtcctcgtt
     1561 tggctgtgcg gcatcgtgga ctaccgtgag catgggcgtg ccttcccgaa ccagcgcgcc
     1621 aacgccggtc ctgatcagag ctacttggac ttcag
//
LOCUS       DAOPRQ010000169         1588 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_60816_length_1588_cov_12.297456, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000169 DAOPRQ010000000
VERSION     DAOPRQ010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60816_length_1588_cov_12.297456"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            205..1443
                     /locus_tag="PKJ61_12515"
     CDS             205..1443
                     /locus_tag="PKJ61_12515"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOC14682.1"
                     /translation="MTELKKAIDAVLDGVTAGTRGVPGVAAAVTDRIGTIYEGARGVR
                     NTASGERFTPDTVCAIFSTTKAIAGTACLQLVEQGLLDLDAPAKEYAPQLGDIMVIDG
                     FGPDGTPKLRPPKRDVTTRHLLTHTAGFGYDFFNETYTRLANEQGQPWVVDASWKSIK
                     TPLLFDPGEQWEYGSNIDWAGMVVEGITGKRLGEVMRTRILDPIGMKDTAFTMTPSMQ
                     ARQATIHAREDRDGSLLPLDGVTLPQEPELHMGGHGLYGTATDYAKFIRMWLNDGRSD
                     TGEQILRPETVEMASRNHLPKGMRVKMLPGVGATATAASILPVSRPRLSNDAEFFPGM
                     SKTWALTFMINEEPAPTGRPAGSLAWAGLANLYYWIDRANGLGGYWATQIFPFADPVS
                     VEGFLAMEKAAYQHAKPRTR"
BASE COUNT          290 a          524 c          535 g          239 t
ORIGIN      
        1 ctagggccaa ccgccaagtg gcgggatccg gcccgcctcg ctgcgccggg tggcggttat
       61 cggccttccc gtgagttgat accttctcat cgccacaggc atgccgtcgg agctgcatgg
      121 cgcgagggca gcgtcccgcg cgtcatcgga ggtccagcga ccggcaccga cctgcggcgg
      181 taactcaata ccacggagac gaaaatgacg gaactgaaga aggcgatcga cgccgttctc
      241 gacggcgtga cggcggggac gcggggcgtg cccggcgtcg ctgcggcggt gaccgaccgc
      301 atcggcacca tctacgaagg cgcgcgcggt gtgcgcaaca ccgcgtccgg cgagcgcttc
      361 acaccggaca cggtgtgcgc catcttctcg accaccaagg ccatcgccgg aaccgcctgc
      421 ctgcagctgg tcgaacaggg tttgctcgac ctcgacgcac ctgccaagga gtacgcgccc
      481 cagctgggcg acatcatggt gatcgacggc ttcggccccg acgggacacc caagctgcgc
      541 ccgccgaagc gcgacgtgac cacgcgtcat ctgctcacgc acacagcagg ctttggctac
      601 gacttcttca acgaaacgta cacgcgcctc gccaacgagc agggccagcc ctgggtggtc
      661 gatgcgtcgt ggaagtcgat caagacgccg ctgctgttcg atccgggcga gcaatgggag
      721 tacggcagca acatcgattg ggccggaatg gtggtcgagg gcatcaccgg caagcggctc
      781 ggcgaggtca tgcgcacgag gattctcgat ccgatcggga tgaaggacac cgccttcacg
      841 atgaccccgt cgatgcaggc ccgacaggcg acgatccatg cgcgggagga tcgggacggg
      901 tcgctcctcc cgctcgacgg ggtgacattg ccgcaggagc cggaactcca catgggaggc
      961 cacggcctgt acgggacggc gacggactac gcgaagttca tccggatgtg gctcaacgac
     1021 gggcgcagcg acaccggaga gcaaatcctg cggccggaga ccgtggagat ggcgtcccgc
     1081 aatcacctgc ccaagggcat gcgcgtcaag atgctgcccg gtgtcggcgc gacggcgaca
     1141 gcggcttcca tcctgccggt gtcgaggccg cggctgtcca acgatgccga attcttcccc
     1201 ggcatgtcga agacctgggc gctcacgttc atgatcaacg aggaaccggc gcccacgggc
     1261 cggcctgccg gatctcttgc gtgggccggc ctcgccaacc tctactactg gatcgaccgt
     1321 gcgaacggcc tgggcgggta ttgggcgacc cagatcttcc cgtttgccga cccggtctcg
     1381 gtcgaaggat tccttgcgat ggagaaggcg gcgtatcagc acgcgaagcc tcgcactcgg
     1441 taactgacgc cgagccgatc caccgcggac agcgaagccg atcgtcgcgg ccaagtcact
     1501 cctgggagcc agtctcggac tggcgccatg gagtgcctag ggcagcgacg tctcgccaca
     1561 cgggcggtcg agtttgggga gcactcgt
//
LOCUS       DAOPRQ010000170         1577 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_61439_length_1577_cov_12.683968, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000170 DAOPRQ010000000
VERSION     DAOPRQ010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61439_length_1577_cov_12.683968"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..650
                     /locus_tag="PKJ61_12520"
     CDS             <1..650
                     /locus_tag="PKJ61_12520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14683.1"
                     /translation="NRSTLEARDPWAIITHAVRITCIYEERAQGLLCSVHQARRSHVS
                     AFHDPERFSDRDTALADYHPAFHVSDDLPSDAESDPHDLLGSAQACMSATSAAEDAIA
                     LLCLLNWPADIARASVEHVCGALAKAGTRQSAYEALRRDRHARALLDLPRRTWTALLR
                     ALLGNPHPAYASTSAGRGVLLRLLLGETLELLLRDDDLILALAIGAPRTRGGEQR"
     gene            647..>1577
                     /locus_tag="PKJ61_12525"
     CDS             647..>1577
                     /locus_tag="PKJ61_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HOC14684.1"
                     /translation="MTDPRTGIQLDRAVDSLIVGKRHRTDLGDIDALAASIDRDGLLQ
                     PLTITIDGVLVCGARRLAAIKMLGWRTVSVWVRGGLSDRLGKLLAEQDDNMLHKPYNQ
                     LEAAGLYREIKEVMAEDAARRQASTRFSAENQPGNDGGGNFPPPSDGPTGKAREQAAA
                     MIPGGASYKTLEKVGYIEEIAANPAQPETLRAEAAAALERIEAGDPVHPLYQAIRESA
                     DAAREVREATMHALAEDAVARANAMKKGKKPKARPKPLVPDDAPPVRYPVRAFVQTWG
                     ELASWWTHYDIDVLAVELTDDQYENFLTVAEATA"
BASE COUNT          274 a          576 c          500 g          227 t
ORIGIN      
        1 agaaccgctc caccctcgaa gcccgcgatc cgtgggcgat catcacccac gccgtacgga
       61 tcacctgcat ctacgaagaa cgcgcccaag gcctgctctg ctcggtgcac caggcccgcc
      121 gctcccacgt ttcggcgttc cacgaccctg aacggttctc cgaccgcgac accgccctgg
      181 ccgactacca tcccgccttc cacgtctctg acgatctgcc cagcgacgcc gagtccgatc
      241 cgcatgatct gctggggtcg gcgcaggcct gcatgtcggc cacgtcagct gccgaggacg
      301 cgatcgcgct gctgtgcctg ctcaactggc ctgctgatat tgcgcgggct tcggtcgagc
      361 atgtctgcgg cgcactcgcc aaagccggaa cccgccaatc tgcctatgag gcgttgcggc
      421 gcgatcggca cgctcgcgcg ctgctcgatc taccccgccg cacctggaca gcgctgctga
      481 gggctctgct cggcaacccg caccccgcct acgcctcaac cagcgccggg cgcggggtgc
      541 tgctgcggct cctgctgggc gagaccttgg aactgttgct gcgcgacgac gacctgatcc
      601 tcgccctcgc catcggggcc cccagaaccc ggggtggtga gcagagatga ccgacccgcg
      661 caccggcatc cagctcgacc gggccgtgga ttccctgatc gtcggcaagc gccaccgcac
      721 cgacctcggc gacatcgatg cgcttgccgc gtccatcgac cgcgacggcc tgcttcagcc
      781 gttgacgatc accatcgacg gcgtgctggt ctgcggggcg cgccgcctgg cggcgatcaa
      841 gatgctcggc tggcgcaccg tcagcgtgtg ggtccgcggt ggcctgtcgg atcggcttgg
      901 gaagctcctg gctgagcagg acgacaacat gctccacaag ccttacaacc agctcgaagc
      961 ggccggcctc taccgcgaga tcaaggaagt catggccgaa gacgccgccc gccgccaagc
     1021 ctcgacgcgg ttcagcgccg agaaccagcc aggaaatgac ggtggtggaa attttccacc
     1081 accgtcagac ggcccgaccg ggaaggcgcg ggaacaggcc gcggcgatga tccccggcgg
     1141 tgcgtcgtac aagacgttgg agaaggtcgg ctacatcgag gagatcgccg ccaaccccgc
     1201 ccaacccgag acgctccgcg ccgaagcggc cgcggcgttg gagcggatcg aggcaggcga
     1261 cccggtgcat ccgctctacc aggcgatccg tgagagcgcc gatgctgcgc gtgaggtgcg
     1321 ggaggcgacg atgcacgcgc tcgccgagga tgccgtcgcc cgcgccaacg cgatgaagaa
     1381 gggcaagaag cccaaggccc gccccaagcc actcgtgccc gacgacgcac cgccagtgag
     1441 gtatccggta agggcgttcg tccagacctg gggcgaactc gccagttggt ggacccacta
     1501 cgacatcgac gtgctcgccg tcgagctgac cgacgaccag tacgagaact tcctcaccgt
     1561 cgccgaagcc accgccc
//
LOCUS       DAOPRQ010000171         1566 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_62062_length_1566_cov_30.155526, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000171 DAOPRQ010000000
VERSION     DAOPRQ010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62062_length_1566_cov_30.155526"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            45..1019
                     /locus_tag="PKJ61_12530"
     CDS             45..1019
                     /locus_tag="PKJ61_12530"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOC14685.1"
                     /translation="MSSTRPECSSGTENVNRHAEDEEPRAAVFNYVPYAIPKDEWAPV
                     ADFTRESVLLVHPGSERQAIEAMRTVSQFVVWAHREGYPLDRDRMFVPDAVERYIAVA
                     ASHLAVSSRATRRSDLRRFSRQITTKAPWAPDPPQLKQRRTLVPFTQDEITRLWEIAN
                     DQSTKIRRRRFTGYLALGLGAGLHPHEMWKANVGDLETVHGFLCVRVTESRHPRVVPI
                     TAPNDEILTRFLTEPPDAPILAYRPRKWDRSPFHEMLRKADVPPGCPTITPARLRATW
                     MVDHLTHGMSMGGLTTLAGFTSWRSYQPLQQYIPQPDEPTLFAQAVRR"
     gene            1058..>1566
                     /locus_tag="PKJ61_12535"
     CDS             1058..>1566
                     /locus_tag="PKJ61_12535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14686.1"
                     /translation="MSSDATPANPMTDEQRIARAVAVATSEIAVKLCAAAERRTGRPR
                     LIPLAGVLAAYIFHATGEPHTMTTSAVCRSIKKLKPRQRAALGIPKRMQVRYKRMHSG
                     WRAIKRVLEHGVLVEHDHDLAVDPDTGEVLPCPGGCPFEQVGLDELTTLLIQASLPPE
                     FERPPAVAI"
BASE COUNT          268 a          568 c          490 g          240 t
ORIGIN      
        1 gctgccttca ttcacttgcg caagcaccct gagggggtac ttctatgtct tcaacacgtc
       61 cagagtgctc ttctggaact gagaacgtca accgccacgc cgaggatgag gagcctcgcg
      121 ccgcggtctt caactacgtg ccgtacgcga tccccaagga cgagtgggca cctgtcgcgg
      181 acttcacccg cgagtccgtc cttcttgtcc accccgggag tgaacgacag gccatcgagg
      241 cgatgcggac cgtctcgcag ttcgtcgtct gggcacaccg tgagggctac ccgctggatc
      301 gggaccgaat gttcgtcccc gacgcggtcg agcgctacat cgcggtcgct gcctcgcact
      361 tggcagtgtc ttcgcgcgcg acccgtcgct cggaccttcg gcggttctcc cgccagatca
      421 cgacgaaagc gccgtgggca ccggatccgc cgcagctgaa gcagcgcagg acgctcgtgc
      481 cgttcaccca ggacgagatc acgcgcctgt gggagatcgc caacgaccag agcacgaaga
      541 tccgccgtcg ccgcttcacc ggctacctcg cgctcggtct cggcgcgggg ttgcacccac
      601 acgagatgtg gaaggccaac gtcggcgacc tcgagacggt ccatggcttc ctctgcgtcc
      661 gagtgactga gagcaggcat cccagggtcg tgccgatcac cgcacccaac gacgagatcc
      721 tcaccagatt cctcaccgaa ccaccggacg cgccgatcct ggcctaccga ccacggaagt
      781 gggaccgttc accgttccac gagatgctgc gcaaggccga cgtcccgccc ggctgtccga
      841 cgatcacccc ggcccggctg cgggccacct ggatggtcga ccacctcacc cacggcatgt
      901 ccatgggcgg gctcaccacg ctggccggct tcacctcgtg gcggtcctac cagcccctgc
      961 agcagtacat cccgcaaccg gacgagccca cactgttcgc ccaggcggtc cgccggtgag
     1021 ccgatcgcga cggcctccga gcgccgttgg ttcgcgggtg agctcggacg caacgccggc
     1081 caacccgatg acggacgaac agcgcatcgc gcgggccgtc gcggtcgcga ccagcgagat
     1141 cgctgtgaag ctctgcgcgg cggcggaacg gcggaccggg cggcctcgac tcatcccgct
     1201 ggcaggggtc cttgcggcgt atatcttcca cgccaccggc gagccgcaca cgatgaccac
     1261 ctccgctgta tgccgatcca tcaagaagct gaagccgagg caacgagcgg cgctcgggat
     1321 cccgaagcgc atgcaggtcc gctacaagcg gatgcactcc ggctggcggg ccatcaaacg
     1381 cgtgctcgag catggtgtcc tggtcgagca cgaccatgac ctcgccgtcg acccggacac
     1441 cggcgaagtc ctgccctgcc cggggggttg cccgttcgag caggtcggcc tggacgagct
     1501 cacgactctg ctcatccagg ccagcctgcc gccggagttc gagcgaccgc cggcggtggc
     1561 catcga
//
LOCUS       DAOPRQ010000172         1553 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Propionicimonas sp. isolate AMR_MDS_1492
            NODE_62777_length_1553_cov_15.512016, whole genome shotgun
            sequence.
ACCESSION   DAOPRQ010000172 DAOPRQ010000000
VERSION     DAOPRQ010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854697
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Propionicimonas sp. (wastewater metagenome)
  ORGANISM  Propionicimonas sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Propionicimonas.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:20:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,471
            Genes (coding)                    :: 2,442
            CDSs (with protein)               :: 2,442
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 0 of 29
            Pseudo Genes (incomplete)         :: 27 of 29
            Pseudo Genes (internal stop)      :: 3 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Propionicimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62777_length_1553_cov_15.512016"
                     /isolate="AMR_MDS_1492"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1955623"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(198..728)
                     /locus_tag="PKJ61_12540"
     CDS             complement(198..728)
                     /locus_tag="PKJ61_12540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14687.1"
                     /translation="MNLEELRTQYHDLAVTACEWRAMGLADPHDMAAEVFGRLDSTKD
                     HGLRDLYASIERVVVLSFQRNAERSSIMDRLRGGVFTERTAPIPGGDFLKALSNLRGR
                     DRNILQYRFWDELTDLETAEVLNTTVEQVIERLATAGSRYLGKLSRSHPDLNLSDIAD
                     TLRSIKPGIYGRYGMG"
     gene            757..>1553
                     /locus_tag="PKJ61_12545"
     CDS             757..>1553
                     /locus_tag="PKJ61_12545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC14688.1"
                     /translation="MSEPTSSADARTIVRAASHLWDAVSAALGELQRNEAAIRRHHDA
                     LLDDLADRRLAELPLTALRTSTSGRLSLAPLADAGFHNVRQLVGATEAQLDRLPGIGR
                     PTARAVVAAVAELRRAAREDVRFRIEFDPANREATALLRALARHDELRAAASGVRDDL
                     GELLRWLPGEIEAAKPAANPVRWLFTGASRRPLALASVERLRQGLAVAEQRGVGERAD
                     EIRRLSGARPADPWVDFERRAADYYALLSEFVPTTTPIQAGQGYLTA"
BASE COUNT          215 a          495 c          569 g          274 t
ORIGIN      
        1 gcccggatgg tggtggtgat gctgttgttc gcgccgatcg catcgatgat cgcggcgttc
       61 acggccaagg ccggcctgga cgtggaactt tcggcgttca tgacctcggt gaccctggtg
      121 gtcggcatcg tgatgatgcc gacgctctac ctgctgctcg cctagccttc ggcgggccta
      181 agggccaatt gccaggctca gcccattccg tagcgcccgt agatgccggg cttgatgctg
      241 cgcagggtgt cggcgatgtc gctgaggttc aggtcgggat ggctccggga gagctttccg
      301 agatagcgcg agccggccgt ggccaggcgc tcgatcacct gttcgacggt cgtgttcagc
      361 acttcggcgg tctcaaggtc ggtcagttcg tcccagaagc ggtattgcag gatgttgcgg
      421 tcgcgtccgc gcaggttgct gagcgctttg aggaagtccc cgccggggat gggcgcggtt
      481 cgctcggtga acacaccgcc gcgcagccgg tccatgatgc tggagcgttc ggcgttgcgc
      541 tggaaactga gcaccaccac gcgttcgatg gacgcgtaca ggtcgcgaag gccgtgatcc
      601 ttggtgctgt ccagtcggcc gaacacctcc gcggccatgt cgtgcgggtc ggccagcccc
      661 atggcccgcc attcgcaggc ggtcaccgcg aggtcgtggt actgcgtccg caactcttcg
      721 aggttcacag gacaaacgta acgctagcgt tggcgcgtga gcgagccgac cagtagcgcg
      781 gacgctcgga cgatcgtgcg ggcagccagc cacctctggg acgcggtctc ggccgcgctc
      841 ggcgagctcc agcgcaatga ggcggcgatc cggcggcacc acgacgcgct gctggacgac
      901 ctggccgacc ggcgactggc cgaattgccg ctgaccgcgc tgcgcacttc gacgtccgga
      961 cggctgagct tggctccgtt ggcggacgcg ggattccata acgtccgcca actggtcggg
     1021 gctaccgagg ctcagctcga ccggctgccg gggatcggcc ggccgacagc tcgcgcggtc
     1081 gtggccgccg tggctgagct tcgtcgggcc gcgcgcgagg atgtccgctt ccgaatcgag
     1141 ttcgacccgg cgaaccgtga ggccaccgcc ctgttgcggg cactggccag gcacgacgag
     1201 ctgcgcgcgg ctgcgtccgg ggtgcgtgac gatctgggtg agctgctgcg ctggctgcct
     1261 ggcgagatcg aggccgcaaa gccggctgcg aacccggtgc gctggctgtt caccggcgct
     1321 tcgcggcgtc cgctggcgct ggcttcggtc gagcgcttgc ggcaagggct ggccgtggcc
     1381 gaacagcgtg gcgtcggtga gcgggcggac gaaatccggc ggctgagcgg tgctcgtccg
     1441 gccgatccgt gggtcgattt cgagcgaagg gcggccgact actacgcgct gttgagcgag
     1501 ttcgtgccga cgaccactcc cattcaggcc ggtcagggct acctgaccgc gga
//
